@@ -8,6 +8,14 @@ section: 3.4
8
8
9
9
---
10
10
11
+ <video muted autoplay controls >
12
+ <source src="presentations/miso_samples.mp4" type="video/mp4">
13
+ </video >
14
+
15
+ [ Download the PDF] ({{ '/presentations/samples.pdf' | prepend: site.baseurl }})
16
+
17
+
18
+
11
19
<div id =" toc " >
12
20
Table of Contents
13
21
<ol >
@@ -63,21 +71,21 @@ the Project you created in the last session.
63
71
64
72
## 2.1 Entering a Tissue
65
73
66
- All samples in MISO are entered using the bulk entry screen. We will start by
74
+ All samples in MISO are entered using the bulk entry screen. We will start by
67
75
entering a single Sample for reference tissue from the Identity ` ID1 ` .
68
76
69
77
{% include samples-receiving.md detailed=page.is-detailed quantity=page.quantity %}
70
- 1. _ External Name_ : this is the name of the * individual donor* , whether internal or
71
- external. For example, an external name would be "Morgan" (internally we refer to
78
+ 1. _ External Name_ : this is the name of the * individual donor* , whether internal or
79
+ external. For example, an external name would be "Morgan" (internally we refer to
72
80
her as plain ol' MORG_0001).
73
81
* Enter ` PROJ_ID1 ` in the _ External Name_ field, replacing ` PROJ ` with your project's
74
- short name. Record this in your worksheet. <img src =" pics/blue_pencil.png " >
75
- * Once you enter an external name, MISO will go and find any existing donors with
76
- external name(s) that match this, and will add them to the dropdown menu for the
82
+ short name. Record this in your worksheet. <img src =" pics/blue_pencil.png " >
83
+ * Once you enter an external name, MISO will go and find any existing donors with
84
+ external name(s) that match this, and will add them to the dropdown menu for the
77
85
_ Identity Alias_ field.
78
- * If there are no existing donors with the exact external name, the option of
79
- ` First Receipt (project short name) ` will be available.
80
- * If more than one option is available in the _ Identity Alias_ dropdown, the cell
86
+ * If there are no existing donors with the exact external name, the option of
87
+ ` First Receipt (project short name) ` will be available.
88
+ * If more than one option is available in the _ Identity Alias_ dropdown, the cell
81
89
will be displayed with a light purple background.
82
90
1. Select ` First Receipt (project short name) ` for the _ Identity Alias_ .
83
91
1. _ Donor Sex_ : Select any item from the dropdown.
@@ -100,7 +108,7 @@ lines. For more information about Sample nomenclature, see <a
100
108
href="https://wiki.oicr.on.ca/display/GENOMICS/LIMS+Guidelines#LIMSGuidelines-SampleNomenclature "
101
109
target="_ new">Sample Nomenclature</a > on the GENOMICS wiki.
102
110
103
- Record the alias in your worksheet. <img src =" pics/blue_pencil.png " >
111
+ Record the alias in your worksheet. <img src =" pics/blue_pencil.png " >
104
112
105
113
## 2.2 Automatically created Samples
106
114
@@ -174,12 +182,12 @@ cell. You can also click and drag to only fill in a certain number of cells.
174
182
1 . Fill in the columns in the same way for: _ Sample Type_ , _ Project_ , _ Donor Sex_ ,
175
183
_ Tissue Origin_ , _ Tissue Type_ , _ Times Received_ , _ Tube Number_ , _ Material_ , and
176
184
_ QC Status_ .
177
- 1 . _ Description_ : Copying and pasting from Excel and Word is supported.
185
+ 1 . _ Description_ : Copying and pasting from Excel and Word is supported.
178
186
Copy something from anywhere and press Ctrl+V (Windows & Linux) or Command+V (Mac)
179
187
on your keyboard to paste.
180
188
181
- 1 . _ Matrix Barcode_ : you would normally use a hand-scanner or copy and paste a list of
182
- barcodes from a spreadsheet. For each sample, select a barcode, scan or type it in, and record
189
+ 1 . _ Matrix Barcode_ : you would normally use a hand-scanner or copy and paste a list of
190
+ barcodes from a spreadsheet. For each sample, select a barcode, scan or type it in, and record
183
191
it in your worksheet. <img src =" pics/blue_pencil.png " >
184
192
1 . _ External Name_ : Again, this is the name of the * individual* , whether internal or external.
185
193
Here's a suggested list. Replace ` PROJ ` with your own project short name.
@@ -190,18 +198,18 @@ Here's a suggested list. Replace `PROJ` with your own project short name.
190
198
1 . Because you've already created an Identity with the external name ` PROJ_ID1 ` , it is found as a match
191
199
to the external name you entered for the top row. In the ` Identity Alias ` dropdown menu for the first row,
192
200
select the existing Identity.
193
- 1 . Select ` First Receipt ` from the dropdown menu in each remaining cell of the ` Identity Alias ` column.
201
+ 1 . Select ` First Receipt ` from the dropdown menu in each remaining cell of the ` Identity Alias ` column.
194
202
195
203
Some fields cannot be filled down, so enter each of those separately. If you
196
- have a label with an incrementing number (_ e.g._ , ` 1 ` , ` 2 ` ), enter two rows, select them,
204
+ have a label with an incrementing number (_ e.g._ , ` 1 ` , ` 2 ` ), enter two rows, select them,
197
205
then double click the blue square.
198
206
199
207
1 . _ Secondary ID_ : enter ` BioBankID 2 ` in the second row. Select the _ Secondary Identifier_
200
208
fields for rows 1 and 2, then double click the blue square to fill down rows 3 and 4.
201
209
1 . Click _ Save_ at the upper right hand corner.
202
210
203
211
If everything is correct, the _ Sample Alias_ and _ Sample Name_ will be auto-generated for each row and
204
- the samples will be saved.
212
+ the samples will be saved.
205
213
Record the external name, sample class, alias, and barcode in your worksheet. <img src =" pics/blue_pencil.png " >
206
214
If you navigate back to your _ Edit Project_ page, there should be nine Samples:
207
215
@@ -236,7 +244,7 @@ material of an existing Identity.
236
244
* _ Project_ : Select your project.
237
245
* _ Scientific Name_ : ` Homo sapiens ` .
238
246
* _ External Name_ : ` PROJ_ID2 ` (where ` PROJ ` is your project's short name) .
239
- * _ Identity Alias_ : The existing identity should be selected automatically
247
+ * _ Identity Alias_ : The existing identity should be selected automatically
240
248
in the dropdown menu.
241
249
* _ Tissue Origin_ : ` nn (Unknown) `
242
250
* _ Tissue Type_ : ` R (Reference or non-tumour, non-diseased tissue sample) `
@@ -273,7 +281,7 @@ hidden from the MISO interface.
273
281
# 2.5 Scanning tissues and stocks into a Box
274
282
275
283
In this section we will add the stocks and tissues you just received into your
276
- inbox for further work.
284
+ inbox for further work.
277
285
278
286
{% include inboxes.md %}
279
287
@@ -369,8 +377,8 @@ items.".
369
377
Propagate again from the 4 _ gDNA (stock)_ samples to _ gDNA (aliquot)_ .
370
378
371
379
1 . If you are continuing from the end of section ** 3.3 Bulk Editing** , do not
372
- navigate away from the page.
373
- 1. At the top left of the table after saving samples, click the _ Propagate_ button.
380
+ navigate away from the page.
381
+ 1. At the top left of the table after saving samples, click the _ Propagate_ button.
374
382
1. Enter _ 1_ for the number of replicates (child samples to be created from each parent)
375
383
and _ gDNA (aliquot)_ for the type of child sample. Continue to step 3.
376
384
1 . Otherwise, select samples using the following:
0 commit comments