diff --git a/tools/ci_build/github/linux/docker/scripts/manylinux/requirements.txt b/tools/ci_build/github/linux/docker/scripts/manylinux/requirements.txt
index 8dc7b5d6c9f2c..f6c93e427b968 100644
--- a/tools/ci_build/github/linux/docker/scripts/manylinux/requirements.txt
+++ b/tools/ci_build/github/linux/docker/scripts/manylinux/requirements.txt
@@ -4,8 +4,7 @@ mypy
 pytest
 setuptools>=68.2.2
 wheel
-protobuf==4.25.1; python_version < "3.14"
-protobuf==6.33.0; python_version >= "3.14"
+protobuf==6.33.0
 sympy==1.14
 flatbuffers
 neural-compressor>=2.2.1
diff --git a/tools/ci_build/github/linux/docker/scripts/requirements.txt b/tools/ci_build/github/linux/docker/scripts/requirements.txt
index 289e69a5554e0..672efe7a3eaf7 100644
--- a/tools/ci_build/github/linux/docker/scripts/requirements.txt
+++ b/tools/ci_build/github/linux/docker/scripts/requirements.txt
@@ -8,8 +8,7 @@ wheel==0.45.1
 argparse
 sympy==1.14
 flatbuffers
-protobuf==4.25.1; python_version < "3.14"
-protobuf==6.33.0; python_version >= "3.14"
+protobuf==6.33.0
 packaging
 onnxscript==0.5.3; python_version < "3.14"
 onnx-ir==0.1.10; python_version < "3.14"
diff --git a/tools/ci_build/github/linux/python/requirements.txt b/tools/ci_build/github/linux/python/requirements.txt
index 15b2b41f0a02e..7134b64cafb0a 100644
--- a/tools/ci_build/github/linux/python/requirements.txt
+++ b/tools/ci_build/github/linux/python/requirements.txt
@@ -4,8 +4,7 @@ mypy
 pytest
 setuptools>=68.2.2
 wheel
-protobuf==4.25.1; python_version < "3.14"
-protobuf==6.33.0; python_version >= "3.14"
+protobuf==6.33.0
 sympy==1.14
 flatbuffers
 psutil
diff --git a/tools/ci_build/github/windows/python/requirements.txt b/tools/ci_build/github/windows/python/requirements.txt
index 0eb18f5995a8a..04b16225e4307 100644
--- a/tools/ci_build/github/windows/python/requirements.txt
+++ b/tools/ci_build/github/windows/python/requirements.txt
@@ -4,8 +4,7 @@ mypy
 pytest
 setuptools>=68.2.2
 wheel
-protobuf==4.25.1; python_version < "3.14"
-protobuf==6.33.0; python_version >= "3.14"
+protobuf==6.33.0
 sympy==1.14
 flatbuffers
 psutil
diff --git a/tools/ci_build/requirements/transformers-test/requirements.txt b/tools/ci_build/requirements/transformers-test/requirements.txt
index d35580cffd0da..e95509c7ddec3 100644
--- a/tools/ci_build/requirements/transformers-test/requirements.txt
+++ b/tools/ci_build/requirements/transformers-test/requirements.txt
@@ -1,8 +1,7 @@
 # packages used by transformers python unittest
 packaging
 # protobuf and numpy is same as tools/ci_build/github/linux/docker/scripts/manylinux/requirements.txt
-protobuf==4.25.1; python_version < "3.14"
-protobuf==6.33.0; python_version >= "3.14"
+protobuf==6.33.0
 numpy==2.2.6; python_version < "3.14"
 numpy==2.3.2; python_version >= "3.14"
 torch==2.8.0