diff --git a/change/@fluentui-web-components-5ceb07c6-c107-4b38-9f71-d5a4923fe23e.json b/change/@fluentui-web-components-5ceb07c6-c107-4b38-9f71-d5a4923fe23e.json
new file mode 100644
index 00000000000000..e2ac9f3b43f1de
--- /dev/null
+++ b/change/@fluentui-web-components-5ceb07c6-c107-4b38-9f71-d5a4923fe23e.json
@@ -0,0 +1,7 @@
+{
+  "type": "patch",
+  "comment": "fix: remove padding-block-start in multiline dismiss slot to fix alignment",
+  "packageName": "@fluentui/web-components",
+  "email": "jibinjose884@gmail.com",
+  "dependentChangeType": "patch"
+}
diff --git a/packages/web-components/src/message-bar/message-bar.styles.ts b/packages/web-components/src/message-bar/message-bar.styles.ts
index 4eea9e81f59831..e61676540a84bd 100644
--- a/packages/web-components/src/message-bar/message-bar.styles.ts
+++ b/packages/web-components/src/message-bar/message-bar.styles.ts
@@ -117,7 +117,6 @@ export const styles: ElementStyles = css`
   :host([layout='multiline']) ::slotted([slot='dismiss']) {
     align-items: start;
     height: 100%;
-    padding-block-start: ${spacingVerticalS};
   }
 
   ::slotted(*) {