diff --git a/change/@fluentui-react-c9b264cb-f787-48ed-a936-6359e1efa00e.json b/change/@fluentui-react-c9b264cb-f787-48ed-a936-6359e1efa00e.json
new file mode 100644
index 00000000000000..0ca6fbd71c2ec9
--- /dev/null
+++ b/change/@fluentui-react-c9b264cb-f787-48ed-a936-6359e1efa00e.json
@@ -0,0 +1,7 @@
+{
+  "type": "patch",
+  "comment": "fix(TimePicker): Selection now works in locales that don't use \"am\"/\"pm\" in their time format",
+  "packageName": "@fluentui/react",
+  "email": "behowell@microsoft.com",
+  "dependentChangeType": "patch"
+}
diff --git a/packages/react/src/components/TimePicker/TimePicker.test.tsx b/packages/react/src/components/TimePicker/TimePicker.test.tsx
index c335bfea34912d..6b59c63e10fb8d 100644
--- a/packages/react/src/components/TimePicker/TimePicker.test.tsx
+++ b/packages/react/src/components/TimePicker/TimePicker.test.tsx
@@ -119,6 +119,33 @@ describe('TimePicker', () => {
     expect(formattedSelectedTime).toEqual(expectedTime);
   });
 
+  it('allows time selection in locales that format time without "am/pm"', () => {
+    const { toLocaleTimeString } = Date.prototype;
+    const toLocaleTimeStringMock = jest.spyOn(Date.prototype, 'toLocaleTimeString');
+
+    // Mock toLocaleTimeString to simulate running in a Japanese locale
+    toLocaleTimeStringMock.mockImplementation(function (this: Date, _locales, options) {
+      return toLocaleTimeString.call(this, 'ja-JP', options);
+    });
+
+    const dateAnchor = new Date('February 27, 2023 08:00:00');
+
+    const onChange = jest.fn();
+
+    const { getByRole, getAllByRole } = render(
+      <TimePicker allowFreeform={false} increments={15} dateAnchor={dateAnchor} useHour12 onChange={onChange} />,
+    );
+
+    const timePickerComboBox = getByRole('combobox') as HTMLInputElement;
+    userEvent.click(timePickerComboBox);
+    const timePickerOptions = getAllByRole('option') as HTMLButtonElement[];
+    userEvent.click(timePickerOptions[2], undefined, { skipPointerEventsCheck: true });
+
+    expect(onChange).toHaveBeenLastCalledWith(expect.anything(), new Date('February 27, 2023 08:30:00'));
+
+    toLocaleTimeStringMock.mockRestore();
+  });
+
   it('correctly renders options using default value as date anchor', () => {
     const defaultValue = new Date('April 1, 2023 13:00:00');
 
diff --git a/packages/react/src/components/TimePicker/TimePicker.tsx b/packages/react/src/components/TimePicker/TimePicker.tsx
index 2442d38b065cab..0895a612b9609f 100644
--- a/packages/react/src/components/TimePicker/TimePicker.tsx
+++ b/packages/react/src/components/TimePicker/TimePicker.tsx
@@ -89,6 +89,7 @@ export const TimePicker: React.FunctionComponent<ITimePickerProps> = ({
       return {
         key: formattedTimeString,
         text: optionText,
+        data: option,
       };
     });
   }, [dateStartAnchor, increments, optionsCount, showSeconds, onFormatDate, useHour12]);
@@ -156,8 +157,13 @@ export const TimePicker: React.FunctionComponent<ITimePickerProps> = ({
         setSelectedTime(errorMessageToDisplay ? new Date('invalid') : undefined);
         changedTime = new Date('invalid');
       } else {
-        const timeSelection = (option?.key as string) || input || '';
-        const updatedTime = getDateFromTimeSelection(useHour12, dateStartAnchor, timeSelection);
+        let updatedTime;
+        if (option?.data instanceof Date) {
+          updatedTime = option.data;
+        } else {
+          const timeSelection = (option?.key as string) || input || '';
+          updatedTime = getDateFromTimeSelection(useHour12, dateStartAnchor, timeSelection);
+        }
         setSelectedTime(updatedTime);
         changedTime = updatedTime;
       }