diff --git a/change/@fluentui-react-virtualizer-eeedf712-20cc-43fd-afc3-c4bc3ce8f9d7.json b/change/@fluentui-react-virtualizer-eeedf712-20cc-43fd-afc3-c4bc3ce8f9d7.json
new file mode 100644
index 0000000000000..eb7a69c4e2818
--- /dev/null
+++ b/change/@fluentui-react-virtualizer-eeedf712-20cc-43fd-afc3-c4bc3ce8f9d7.json
@@ -0,0 +1,7 @@
+{
+  "type": "prerelease",
+  "comment": "Fix: Ensure 'start buffer' is margin padded into the non-virtualized space on horizontal layouts",
+  "packageName": "@fluentui/react-virtualizer",
+  "email": "mifraser@microsoft.com",
+  "dependentChangeType": "patch"
+}
diff --git a/packages/react-components/react-virtualizer/src/components/Virtualizer/useVirtualizer.ts b/packages/react-components/react-virtualizer/src/components/Virtualizer/useVirtualizer.ts
index 9f6c4d24b0743..c624eca75bd47 100644
--- a/packages/react-components/react-virtualizer/src/components/Virtualizer/useVirtualizer.ts
+++ b/packages/react-components/react-virtualizer/src/components/Virtualizer/useVirtualizer.ts
@@ -113,7 +113,6 @@ export function useVirtualizer_unstable(props: VirtualizerProps): VirtualizerSta
     // Local updates
     updateChildRows(index);
     updateCurrentItemSizes(index);
-
     // State setters
     setActualIndex(index);
   };
@@ -277,7 +276,7 @@ export function useVirtualizer_unstable(props: VirtualizerProps): VirtualizerSta
   }, [getItemSize, itemSize, numItems]);
 
   const calculateBefore = useCallback(() => {
-    const currentIndex = Math.min(actualIndex, numItems);
+    const currentIndex = Math.min(actualIndex, numItems - 1);
 
     if (!getItemSize) {
       // The missing items from before virtualization starts height
@@ -293,19 +292,19 @@ export function useVirtualizer_unstable(props: VirtualizerProps): VirtualizerSta
   }, [actualIndex, getItemSize, itemSize, numItems]);
 
   const calculateAfter = useCallback(() => {
-    if (numItems === 0) {
+    if (numItems === 0 || actualIndex + virtualizerLength >= numItems) {
       return 0;
     }
 
-    const lastItemIndex = Math.min(actualIndex + virtualizerLength, numItems - 1);
+    const lastItemIndex = Math.min(actualIndex + virtualizerLength, numItems);
     if (!getItemSize) {
       // The missing items from after virtualization ends height
-      const remainingItems = numItems - lastItemIndex - 1;
+      const remainingItems = numItems - lastItemIndex;
       return remainingItems * itemSize;
     }
 
     // Time for custom size calcs
-    return childProgressiveSizes.current[numItems - 1] - childProgressiveSizes.current[lastItemIndex];
+    return childProgressiveSizes.current[numItems - 1] - childProgressiveSizes.current[lastItemIndex - 1];
   }, [actualIndex, getItemSize, itemSize, numItems, virtualizerLength]);
 
   const updateChildRows = useCallback(
diff --git a/packages/react-components/react-virtualizer/src/components/Virtualizer/useVirtualizerStyles.styles.ts b/packages/react-components/react-virtualizer/src/components/Virtualizer/useVirtualizerStyles.styles.ts
index 0f11ce8778d7b..b3fcfd4b20f3f 100644
--- a/packages/react-components/react-virtualizer/src/components/Virtualizer/useVirtualizerStyles.styles.ts
+++ b/packages/react-components/react-virtualizer/src/components/Virtualizer/useVirtualizerStyles.styles.ts
@@ -76,9 +76,9 @@ export const useVirtualizerStyles_unstable = (state: VirtualizerState): Virtuali
     // Column-Reverse
     ...(reversed && !horizontal && { marginTop: `-${bufferPx}` }),
     // Row
-    ...(!reversed && horizontal && { marginLeft: `-${bufferPx}` }),
+    ...(!reversed && horizontal && { marginRight: `-${bufferPx}` }),
     // Row-Reverse
-    ...(reversed && horizontal && { marginRight: `-${bufferPx}` }),
+    ...(reversed && horizontal && { marginLeft: `-${bufferPx}` }),
   };
 
   const afterBuffer = {
diff --git a/packages/react-components/react-virtualizer/src/components/VirtualizerScrollView/useVirtualizerScrollViewStyles.styles.ts b/packages/react-components/react-virtualizer/src/components/VirtualizerScrollView/useVirtualizerScrollViewStyles.styles.ts
index cd0221a8cff35..15e818c0f0cb5 100644
--- a/packages/react-components/react-virtualizer/src/components/VirtualizerScrollView/useVirtualizerScrollViewStyles.styles.ts
+++ b/packages/react-components/react-virtualizer/src/components/VirtualizerScrollView/useVirtualizerScrollViewStyles.styles.ts
@@ -16,11 +16,9 @@ const useStyles = makeStyles({
     display: 'flex',
     width: '100%',
     height: '100%',
-    overflowAnchor: 'none',
   },
   vertical: {
     flexDirection: 'column',
-    overflowAnchor: 'none',
     overflowY: 'auto',
   },
   horizontal: {
diff --git a/packages/react-components/react-virtualizer/src/components/VirtualizerScrollViewDynamic/useVirtualizerScrollViewDynamicStyles.styles.ts b/packages/react-components/react-virtualizer/src/components/VirtualizerScrollViewDynamic/useVirtualizerScrollViewDynamicStyles.styles.ts
index 40c3c2a9b18de..a1a58fb7e6994 100644
--- a/packages/react-components/react-virtualizer/src/components/VirtualizerScrollViewDynamic/useVirtualizerScrollViewDynamicStyles.styles.ts
+++ b/packages/react-components/react-virtualizer/src/components/VirtualizerScrollViewDynamic/useVirtualizerScrollViewDynamicStyles.styles.ts
@@ -18,7 +18,6 @@ const useStyles = makeStyles({
     display: 'flex',
     width: '100%',
     height: '100%',
-    overflowAnchor: 'none',
   },
   vertical: {
     flexDirection: 'column',
diff --git a/packages/react-components/react-virtualizer/src/hooks/useDynamicVirtualizerMeasure.ts b/packages/react-components/react-virtualizer/src/hooks/useDynamicVirtualizerMeasure.ts
index e026991d5801c..91302fe36d734 100644
--- a/packages/react-components/react-virtualizer/src/hooks/useDynamicVirtualizerMeasure.ts
+++ b/packages/react-components/react-virtualizer/src/hooks/useDynamicVirtualizerMeasure.ts
@@ -58,14 +58,14 @@ export const useDynamicVirtualizerMeasure = <TElement extends HTMLElement>(
       /*
        * Number of items to append at each end, i.e. 'preload' each side before entering view.
        */
-      const bufferItems = Math.max(Math.floor(length / 4), 2);
+      const bufferItems = Math.max(Math.floor(length / 4), 4);
 
       /*
        * This is how far we deviate into the bufferItems to detect a redraw.
        */
       const bufferSize = Math.max(Math.floor((length / 8) * defaultItemSize), 1);
 
-      const totalLength = length + bufferItems * 2 + 3;
+      const totalLength = length + bufferItems * 2 + 1;
       setState({
         virtualizerLength: totalLength,
         virtualizerBufferSize: bufferSize,
diff --git a/packages/react-components/react-virtualizer/stories/Virtualizer/Default.stories.tsx b/packages/react-components/react-virtualizer/stories/Virtualizer/Default.stories.tsx
index 28b75b3f13a2f..a1e8b98ff9145 100644
--- a/packages/react-components/react-virtualizer/stories/Virtualizer/Default.stories.tsx
+++ b/packages/react-components/react-virtualizer/stories/Virtualizer/Default.stories.tsx
@@ -6,7 +6,6 @@ const useStyles = makeStyles({
   container: {
     display: 'flex',
     flexDirection: 'column',
-    overflowAnchor: 'none',
     overflowY: 'auto',
     width: '100%',
     height: '100%',
diff --git a/packages/react-components/react-virtualizer/stories/Virtualizer/DefaultUnbounded.stories.tsx b/packages/react-components/react-virtualizer/stories/Virtualizer/DefaultUnbounded.stories.tsx
index 799190d010d26..a22d4758951ae 100644
--- a/packages/react-components/react-virtualizer/stories/Virtualizer/DefaultUnbounded.stories.tsx
+++ b/packages/react-components/react-virtualizer/stories/Virtualizer/DefaultUnbounded.stories.tsx
@@ -14,7 +14,6 @@ const useStyles = makeStyles({
      */
     display: 'flex',
     flexDirection: 'column',
-    overflowAnchor: 'none',
     maxHeight: '300VH',
     width: '100%',
     height: '100%',
diff --git a/packages/react-components/react-virtualizer/stories/Virtualizer/Dynamic.stories.tsx b/packages/react-components/react-virtualizer/stories/Virtualizer/Dynamic.stories.tsx
index f89975410c0e7..6705c92cf2a9b 100644
--- a/packages/react-components/react-virtualizer/stories/Virtualizer/Dynamic.stories.tsx
+++ b/packages/react-components/react-virtualizer/stories/Virtualizer/Dynamic.stories.tsx
@@ -13,7 +13,6 @@ const useStyles = makeStyles({
   container: {
     display: 'flex',
     flexDirection: 'column',
-    overflowAnchor: 'none',
     overflowY: 'auto',
     width: '100%',
     height: '100%',
diff --git a/packages/react-components/react-virtualizer/stories/Virtualizer/Horizontal.stories.tsx b/packages/react-components/react-virtualizer/stories/Virtualizer/Horizontal.stories.tsx
index 9b0539346f338..16edbe43f39f4 100644
--- a/packages/react-components/react-virtualizer/stories/Virtualizer/Horizontal.stories.tsx
+++ b/packages/react-components/react-virtualizer/stories/Virtualizer/Horizontal.stories.tsx
@@ -6,7 +6,6 @@ const useStyles = makeStyles({
   container: {
     display: 'flex',
     flexDirection: 'row',
-    overflowAnchor: 'none',
     overflowY: 'auto',
     width: '100%',
     height: '100%',
diff --git a/packages/react-components/react-virtualizer/stories/Virtualizer/MultiUnbounded.stories.tsx b/packages/react-components/react-virtualizer/stories/Virtualizer/MultiUnbounded.stories.tsx
index 53bab4dffe408..e6bf79745fd88 100644
--- a/packages/react-components/react-virtualizer/stories/Virtualizer/MultiUnbounded.stories.tsx
+++ b/packages/react-components/react-virtualizer/stories/Virtualizer/MultiUnbounded.stories.tsx
@@ -12,7 +12,6 @@ const useStyles = makeStyles({
      */
     display: 'flex',
     flexDirection: 'column',
-    overflowAnchor: 'none',
     maxHeight: '300VH',
     width: '100%',
     height: '100%',
diff --git a/packages/react-components/react-virtualizer/stories/Virtualizer/RTL.stories.tsx b/packages/react-components/react-virtualizer/stories/Virtualizer/RTL.stories.tsx
index 9f6d296753b31..dcb16f951cb12 100644
--- a/packages/react-components/react-virtualizer/stories/Virtualizer/RTL.stories.tsx
+++ b/packages/react-components/react-virtualizer/stories/Virtualizer/RTL.stories.tsx
@@ -6,7 +6,6 @@ const useStyles = makeStyles({
   container: {
     display: 'flex',
     flexDirection: 'row',
-    overflowAnchor: 'none',
     overflowY: 'auto',
     width: '100%',
     height: '100%',
diff --git a/packages/react-components/react-virtualizer/stories/Virtualizer/Reversed.stories.tsx b/packages/react-components/react-virtualizer/stories/Virtualizer/Reversed.stories.tsx
index a774d23f4321c..109f2da1f82c9 100644
--- a/packages/react-components/react-virtualizer/stories/Virtualizer/Reversed.stories.tsx
+++ b/packages/react-components/react-virtualizer/stories/Virtualizer/Reversed.stories.tsx
@@ -6,7 +6,6 @@ const useStyles = makeStyles({
   container: {
     display: 'flex',
     flexDirection: 'column-reverse',
-    overflowAnchor: 'none',
     overflowY: 'auto',
     width: '100%',
     height: '100%',
diff --git a/packages/react-components/react-virtualizer/stories/Virtualizer/ReversedHorizontal.stories.tsx b/packages/react-components/react-virtualizer/stories/Virtualizer/ReversedHorizontal.stories.tsx
index 8d0ca6180070d..bdb10df418cf6 100644
--- a/packages/react-components/react-virtualizer/stories/Virtualizer/ReversedHorizontal.stories.tsx
+++ b/packages/react-components/react-virtualizer/stories/Virtualizer/ReversedHorizontal.stories.tsx
@@ -6,7 +6,6 @@ const useStyles = makeStyles({
   container: {
     display: 'flex',
     flexDirection: 'row-reverse',
-    overflowAnchor: 'none',
     overflowY: 'auto',
     width: '100%',
     height: '100%',
diff --git a/packages/react-components/react-virtualizer/stories/VirtualizerScrollView/SnapToAlignment.stories.tsx b/packages/react-components/react-virtualizer/stories/VirtualizerScrollView/SnapToAlignment.stories.tsx
new file mode 100644
index 0000000000000..3c28373410a70
--- /dev/null
+++ b/packages/react-components/react-virtualizer/stories/VirtualizerScrollView/SnapToAlignment.stories.tsx
@@ -0,0 +1,57 @@
+import { Button, makeStyles, shorthands, useArrowNavigationGroup } from '@fluentui/react-components';
+import * as React from 'react';
+import { VirtualizerScrollView } from '@fluentui/react-components/unstable';
+
+const useStyles = makeStyles({
+  container: {
+    maxHeight: '300px',
+    width: '100%',
+    maxWidth: '100%',
+    scrollSnapType: 'x mandatory',
+    scrollBehavior: 'auto',
+    ...shorthands.padding('10px', '2px'),
+    ...shorthands.gap('10px'),
+  },
+  child: {
+    scrollSnapAlign: 'start',
+    height: '100px',
+    width: '100px',
+    midWidth: '100px',
+  },
+  button: {
+    width: '100%',
+    height: '100%',
+  },
+});
+
+export const SnapToAlignment = () => {
+  const styles = useStyles();
+  const childLength = 1000;
+  const attributes = useArrowNavigationGroup({
+    axis: 'horizontal',
+    memorizeCurrent: true,
+  });
+
+  return (
+    <VirtualizerScrollView
+      numItems={childLength}
+      itemSize={100}
+      axis="horizontal"
+      container={{ role: 'list', className: styles.container, ...attributes }}
+    >
+      {(index: number) => {
+        return (
+          <div
+            role={'listitem'}
+            aria-posinset={index}
+            aria-setsize={childLength}
+            key={`test-virtualizer-child-${index}`}
+            className={styles.child}
+          >
+            <Button className={styles.button}>{`Node-${index}`}</Button>
+          </div>
+        );
+      }}
+    </VirtualizerScrollView>
+  );
+};
diff --git a/packages/react-components/react-virtualizer/stories/VirtualizerScrollView/index.stories.ts b/packages/react-components/react-virtualizer/stories/VirtualizerScrollView/index.stories.ts
index 456f3e7ae733f..82d353041b649 100644
--- a/packages/react-components/react-virtualizer/stories/VirtualizerScrollView/index.stories.ts
+++ b/packages/react-components/react-virtualizer/stories/VirtualizerScrollView/index.stories.ts
@@ -3,6 +3,7 @@ import descriptionMd from './VirtualizerScrollViewDescription.md';
 
 export { Default } from './Default.stories';
 export { ScrollTo } from './ScrollTo.stories';
+export { SnapToAlignment } from './SnapToAlignment.stories';
 
 export default {
   title: 'Preview Components/VirtualizerScrollView',
diff --git a/packages/react-components/react-virtualizer/stories/VirtualizerScrollViewDynamic/Default.stories.tsx b/packages/react-components/react-virtualizer/stories/VirtualizerScrollViewDynamic/Default.stories.tsx
index 6c804c6c2cef6..63fff1f9724f8 100644
--- a/packages/react-components/react-virtualizer/stories/VirtualizerScrollViewDynamic/Default.stories.tsx
+++ b/packages/react-components/react-virtualizer/stories/VirtualizerScrollViewDynamic/Default.stories.tsx
@@ -23,7 +23,7 @@ export const Default = () => {
   useEffect(() => {
     let _totalSize = 0;
     for (let i = 0; i < childLength; i++) {
-      arraySize.current[i] = Math.random() * 150 + minHeight;
+      arraySize.current[i] = Math.floor(Math.random() * 150 + minHeight);
       _totalSize += arraySize.current[i];
     }
     setTotalSize(_totalSize);