diff --git a/change/@fluentui-react-checkbox-4ebdfb9d-a372-4ce7-99f4-c66d376885b1.json b/change/@fluentui-react-checkbox-4ebdfb9d-a372-4ce7-99f4-c66d376885b1.json
new file mode 100644
index 00000000000000..b128033116e482
--- /dev/null
+++ b/change/@fluentui-react-checkbox-4ebdfb9d-a372-4ce7-99f4-c66d376885b1.json
@@ -0,0 +1,7 @@
+{
+  "type": "patch",
+  "comment": "fix: Fix vertical alignment of inline Checkbox when checked vs. unchecked",
+  "packageName": "@fluentui/react-checkbox",
+  "email": "behowell@microsoft.com",
+  "dependentChangeType": "patch"
+}
diff --git a/packages/react-components/react-checkbox/src/components/Checkbox/useCheckboxStyles.ts b/packages/react-components/react-checkbox/src/components/Checkbox/useCheckboxStyles.ts
index 0eb3cffda9e34c..cccbf7712cd8d0 100644
--- a/packages/react-components/react-checkbox/src/components/Checkbox/useCheckboxStyles.ts
+++ b/packages/react-components/react-checkbox/src/components/Checkbox/useCheckboxStyles.ts
@@ -19,6 +19,7 @@ const useRootBaseClassName = makeResetStyles({
   position: 'relative',
   display: 'inline-flex',
   cursor: 'pointer',
+  verticalAlign: 'middle',
   color: tokens.colorNeutralForeground3,
   ...createFocusOutlineStyle({ style: {}, selector: 'focus-within' }),
 });