diff --git a/change/@fluentui-react-popover-e33a0b34-bcda-454b-be5e-8ca39b89cf3c.json b/change/@fluentui-react-popover-e33a0b34-bcda-454b-be5e-8ca39b89cf3c.json
new file mode 100644
index 00000000000000..759960c5ca9b79
--- /dev/null
+++ b/change/@fluentui-react-popover-e33a0b34-bcda-454b-be5e-8ca39b89cf3c.json
@@ -0,0 +1,7 @@
+{
+  "type": "patch",
+  "comment": "fix: Popover without focus trap should not be aria-hidden",
+  "packageName": "@fluentui/react-popover",
+  "email": "lingfangao@hotmail.com",
+  "dependentChangeType": "patch"
+}
diff --git a/packages/react-components/react-popover/src/components/PopoverSurface/__snapshots__/PopoverSurface.test.tsx.snap b/packages/react-components/react-popover/src/components/PopoverSurface/__snapshots__/PopoverSurface.test.tsx.snap
index 5bf2a8aeea3920..d5445f25375b23 100644
--- a/packages/react-components/react-popover/src/components/PopoverSurface/__snapshots__/PopoverSurface.test.tsx.snap
+++ b/packages/react-components/react-popover/src/components/PopoverSurface/__snapshots__/PopoverSurface.test.tsx.snap
@@ -4,7 +4,7 @@ exports[`PopoverSurface renders a default state 1`] = `
 <div
   aria-label="test"
   class="fui-PopoverSurface"
-  data-tabster="{\\"deloser\\":{},\\"modalizer\\":{\\"id\\":\\"modal-1\\",\\"isOthersAccessible\\":true}}"
+  data-tabster="{\\"deloser\\":{},\\"modalizer\\":{\\"id\\":\\"modal-1\\",\\"isOthersAccessible\\":true,\\"isAlwaysAccessible\\":true}}"
   data-testid="component"
   role="group"
 >
diff --git a/packages/react-components/react-popover/src/components/PopoverSurface/usePopoverSurface.ts b/packages/react-components/react-popover/src/components/PopoverSurface/usePopoverSurface.ts
index abf06c0b0dd0d1..370465d6c4d157 100644
--- a/packages/react-components/react-popover/src/components/PopoverSurface/usePopoverSurface.ts
+++ b/packages/react-components/react-popover/src/components/PopoverSurface/usePopoverSurface.ts
@@ -28,7 +28,7 @@ export const usePopoverSurface_unstable = (
   const trapFocus = usePopoverContext_unstable(context => context.trapFocus);
   const legacyTrapFocus = usePopoverContext_unstable(context => context.legacyTrapFocus);
   const inline = usePopoverContext_unstable(context => context.inline);
-  const { modalAttributes } = useModalAttributes({ trapFocus, legacyTrapFocus });
+  const { modalAttributes } = useModalAttributes({ trapFocus, legacyTrapFocus, alwaysFocusable: !trapFocus });
 
   const state: PopoverSurfaceState = {
     inline,