diff --git a/autogen/coding/local_commandline_code_executor.py b/autogen/coding/local_commandline_code_executor.py
index 29172bbe9221..620b359a4aee 100644
--- a/autogen/coding/local_commandline_code_executor.py
+++ b/autogen/coding/local_commandline_code_executor.py
@@ -294,10 +294,11 @@ def _execute_code_dont_check_setup(self, code_blocks: List[CodeBlock]) -> Comman
             env = os.environ.copy()
 
             if self._virtual_env_context:
-                path_with_virtualenv = rf"{self._virtual_env_context.bin_path}{os.pathsep}{env['PATH']}"
+                virtual_env_abs_path = os.path.abspath(self._virtual_env_context.bin_path)
+                path_with_virtualenv = rf"{virtual_env_abs_path}{os.pathsep}{env['PATH']}"
                 env["PATH"] = path_with_virtualenv
                 if WIN32:
-                    activation_script = os.path.join(self._virtual_env_context.bin_path, "activate.bat")
+                    activation_script = os.path.join(virtual_env_abs_path, "activate.bat")
                     cmd = [activation_script, "&&", *cmd]
 
             try:
diff --git a/test/coding/test_commandline_code_executor.py b/test/coding/test_commandline_code_executor.py
index 4daf2e21bcb2..99c564b42a48 100644
--- a/test/coding/test_commandline_code_executor.py
+++ b/test/coding/test_commandline_code_executor.py
@@ -1,4 +1,5 @@
 import os
+import shutil
 import sys
 import tempfile
 import uuid
@@ -411,3 +412,30 @@ def test_local_executor_with_custom_python_env():
 
         assert execution.exit_code == 0
         assert execution.output.strip() == "True"
+
+
+def test_local_executor_with_custom_python_env_in_local_relative_path():
+    try:
+        relative_folder_path = "temp_folder"
+        if not os.path.isdir(relative_folder_path):
+            os.mkdir(relative_folder_path)
+
+        venv_path = os.path.join(relative_folder_path, ".venv")
+        env_builder = venv.EnvBuilder(with_pip=True)
+        env_builder.create(venv_path)
+        env_builder_context = env_builder.ensure_directories(venv_path)
+
+        executor = LocalCommandLineCodeExecutor(work_dir=relative_folder_path, virtual_env_context=env_builder_context)
+        code_blocks = [
+            CodeBlock(code="import sys; print(sys.executable)", language="python"),
+        ]
+        execution = executor.execute_code_blocks(code_blocks)
+
+        assert execution.exit_code == 0
+
+        # Check if the expected venv is used
+        bin_path = os.path.abspath(env_builder_context.bin_path)
+        assert Path(execution.output.strip()).parent.samefile(bin_path)
+    finally:
+        if os.path.isdir(relative_folder_path):
+            shutil.rmtree(relative_folder_path)