diff --git a/eng/Signing.props b/eng/Signing.props
index bf440e85ece..4604fe0434a 100644
--- a/eng/Signing.props
+++ b/eng/Signing.props
@@ -8,6 +8,9 @@
 
     <!-- add missing entry for .msi, this can be removed once aspire uses arcade 10.0 -->
     <FileExtensionSignInfo Include=".msi" CertificateName="MicrosoftDotNet500" Condition="!@(FileExtensionSignInfo->AnyHaveMetadataValue('Identity', '.msi'))" />
+
+    <!-- Remove .py files from being signed. See https://github.com/dotnet/aspire/issues/13004 -->
+    <FileExtensionSignInfo Remove=".py" />
   </ItemGroup>
 
   <ItemGroup>