diff --git a/.changeset/chatty-flies-report.md b/.changeset/chatty-flies-report.md
new file mode 100644
index 0000000000..53b1a8ea17
--- /dev/null
+++ b/.changeset/chatty-flies-report.md
@@ -0,0 +1,5 @@
+---
+"@lynx-js/web-platform-rsbuild-plugin": patch
+---
+
+Fix build on Windows
diff --git a/packages/web-platform/web-rsbuild-plugin/src/loaders/native-modules.ts b/packages/web-platform/web-rsbuild-plugin/src/loaders/native-modules.ts
index f9930e9d4f..5d3721879d 100644
--- a/packages/web-platform/web-rsbuild-plugin/src/loaders/native-modules.ts
+++ b/packages/web-platform/web-rsbuild-plugin/src/loaders/native-modules.ts
@@ -1,3 +1,9 @@
+// Copyright 2025 The Lynx Authors. All rights reserved.
+// Licensed under the Apache License Version 2.0 that can be found in the
+// LICENSE file in the root directory of this source tree.
+
+import { pathToFileURL } from 'node:url';
+
 import type { LoaderContext } from 'webpack';
 
 interface NativeModulesLoaderOptions {
@@ -12,7 +18,9 @@ export default function(
   const { nativeModulesPath } = options;
   const modifiedSource = source.replace(
     /\/\* LYNX_NATIVE_MODULES_IMPORT \*\//g,
-    `import CUSTOM_NATIVE_MODULES from '${nativeModulesPath}';`,
+    `import CUSTOM_NATIVE_MODULES from ${
+      JSON.stringify(pathToFileURL(nativeModulesPath))
+    };`,
   ).replace(
     /\/\* LYNX_NATIVE_MODULES_ADD \*\//g,
     `Object.entries(CUSTOM_NATIVE_MODULES).map(([moduleName, moduleFunc]) => {
diff --git a/packages/web-platform/web-rsbuild-plugin/src/pluginWebPlatform.ts b/packages/web-platform/web-rsbuild-plugin/src/pluginWebPlatform.ts
index b58b41db34..9d27f6cc68 100644
--- a/packages/web-platform/web-rsbuild-plugin/src/pluginWebPlatform.ts
+++ b/packages/web-platform/web-rsbuild-plugin/src/pluginWebPlatform.ts
@@ -2,11 +2,12 @@
 // Licensed under the Apache License Version 2.0 that can be found in the
 // LICENSE file in the root directory of this source tree.
 
+import path from 'node:path';
+import { fileURLToPath } from 'node:url';
+
 import type { RsbuildPlugin } from '@rsbuild/core';
-import path from 'path';
 
-const __filename = new URL('', import.meta.url).pathname;
-const __dirname = path.dirname(__filename);
+const __dirname = path.dirname(fileURLToPath(import.meta.url));
 
 /**
  * The options for {@link pluginWebPlatform}.
@@ -88,17 +89,13 @@ export function pluginWebPlatform(
       });
 
       api.modifyRspackConfig(rspackConfig => {
-        console.log(path.resolve(
-          __dirname,
-          './loaders/native-modules.js',
-        ));
         rspackConfig.module = {
           ...rspackConfig.module,
           rules: [
             ...(rspackConfig.module?.rules ?? []),
             {
               test:
-                /backgroundThread\/background-apis\/createNativeModules\.js$/,
+                /backgroundThread[\\/]background-apis[\\/]createNativeModules\.js$/,
               loader: path.resolve(
                 __dirname,
                 './loaders/native-modules.js',
diff --git a/packages/web-platform/web-rsbuild-plugin/tests/bundle.test.ts b/packages/web-platform/web-rsbuild-plugin/tests/bundle.test.ts
index 25fd61d07e..867d0b479a 100644
--- a/packages/web-platform/web-rsbuild-plugin/tests/bundle.test.ts
+++ b/packages/web-platform/web-rsbuild-plugin/tests/bundle.test.ts
@@ -47,7 +47,9 @@ describe('Bundle Build', () => {
       for (const m of modules) {
         if (
           m.type === 'javascript/auto'
-          && m.userRequest.includes('tests/fixtures/index.native-modules.ts')
+          && m.userRequest.includes(
+            'tests/fixtures/index.native-modules.ts'.replaceAll('/', path.sep),
+          )
         ) {
           if (!i.isOnlyInitial()) {
             asyncChunkImportCount++;