diff --git a/pkg/activator/net/revision_backends.go b/pkg/activator/net/revision_backends.go
index a8f515872eb4..d802c0137049 100644
--- a/pkg/activator/net/revision_backends.go
+++ b/pkg/activator/net/revision_backends.go
@@ -63,6 +63,7 @@ type revisionDestsUpdate struct {
 const (
 	probeTimeout   time.Duration = 300 * time.Millisecond
 	probeFrequency time.Duration = 200 * time.Millisecond
+	probePath                    = "/_internal/knative/activator/probe"
 )
 
 // revisionWatcher watches the podIPs and ClusterIP of the service for a revision. It implements the logic
@@ -135,6 +136,7 @@ func (rw *revisionWatcher) probe(ctx context.Context, dest string) (bool, error)
 	httpDest := url.URL{
 		Scheme: "http",
 		Host:   dest,
+		Path:   probePath,
 	}
 	// NOTE: changes below may require changes to testing/roundtripper.go to make unit tests passing.
 	return prober.Do(ctx, rw.transport, httpDest.String(),
diff --git a/test/config/100-namespace.yaml b/test/config/100-namespace.yaml
index d763ceee88cd..801f6bbd8c7e 100644
--- a/test/config/100-namespace.yaml
+++ b/test/config/100-namespace.yaml
@@ -21,3 +21,8 @@ apiVersion: v1
 kind: Namespace
 metadata:
   name: serving-tests-alt
+---
+apiVersion: v1
+kind: Namespace
+metadata:
+  name: serving-tests-security
diff --git a/test/config/mtls/destinationrule.yaml b/test/config/security/destinationrule.yaml
similarity index 100%
rename from test/config/mtls/destinationrule.yaml
rename to test/config/security/destinationrule.yaml
diff --git a/test/config/mtls/policy.yaml b/test/config/security/policy.yaml
similarity index 53%
rename from test/config/mtls/policy.yaml
rename to test/config/security/policy.yaml
index 3766d690ba5b..533399c82d6c 100644
--- a/test/config/mtls/policy.yaml
+++ b/test/config/security/policy.yaml
@@ -31,3 +31,19 @@ spec:
   peers:
   - mtls:
       mode: PERMISSIVE
+---
+apiVersion: authentication.istio.io/v1alpha1
+kind: Policy
+metadata:
+  name: default
+  namespace: serving-tests-security
+spec:
+  origins:
+  - jwt:
+      issuer: testing@secure.istio.io
+      jwks: "{ \"keys\":[ {\"e\":\"AQAB\",\"kid\":\"DHFbpoIUqrY8t2zpA2qXfCmr5VO5ZEr4RzHU_-envvQ\",\"kty\":\"RSA\",\"n\":\"xAE7eB6qugXyCAG3yhh7pkDkT65pHymX-P7KfIupjf59vsdo91bSP9C8H07pSAGQO1MV_xFj9VswgsCg4R6otmg5PV2He95lZdHtOcU5DXIg_pbhLdKXbi66GlVeK6ABZOUW3WYtnNHD-91gVuoeJT_DwtGGcp4ignkgXfkiEm4sw-4sfb4qdt5oLbyVpmW6x9cfa7vs2WTfURiCrBoUqgBo_-4WTiULmmHSGZHOjzwa8WtrtOQGsAFjIbno85jp6MnGGGZPYZbDAa_b3y5u-YpW7ypZrvD8BgtKVjgtQgZhLAGezMt0ua3DRrWnKqTZ0BJ_EyxOGuHJrLsn00fnMQ\"}]} "
+      triggerRules:
+      - excludedPaths:
+        - prefix: /metrics
+        - prefix: /_internal/knative/activator/probe
+  principalBinding: USE_ORIGIN
diff --git a/test/e2e-common.sh b/test/e2e-common.sh
index 5df47cbe9aed..8731ce490e1c 100644
--- a/test/e2e-common.sh
+++ b/test/e2e-common.sh
@@ -433,7 +433,8 @@ function test_setup() {
   if (( MESH )); then
     kubectl label namespace serving-tests istio-injection=enabled
     kubectl label namespace serving-tests-alt istio-injection=enabled
-    ko apply ${KO_FLAGS} -f test/config/mtls/ || return 1
+    kubectl label namespace serving-tests-security istio-injection=enabled
+    ko apply ${KO_FLAGS} -f test/config/security/ || return 1
   fi
 
   echo ">> Uploading test images..."
@@ -491,13 +492,15 @@ function test_teardown() {
   echo ">> Removing test resources (test/config/)"
   ko delete --ignore-not-found=true --now -f test/config/
   if (( MESH )); then
-    ko delete --ignore-not-found=true --now -f test/config/mtls/
+    ko delete --ignore-not-found=true --now -f test/config/security/
   fi
   echo ">> Ensuring test namespaces are clean"
   kubectl delete all --all --ignore-not-found --now --timeout 60s -n serving-tests
   kubectl delete --ignore-not-found --now --timeout 60s namespace serving-tests
   kubectl delete all --all --ignore-not-found --now --timeout 60s -n serving-tests-alt
   kubectl delete --ignore-not-found --now --timeout 60s namespace serving-tests-alt
+  kubectl delete all --all --ignore-not-found --now --timeout 60s -n serving-tests-security
+  kubectl delete --ignore-not-found --now --timeout 60s namespace serving-tests-security
 }
 
 # Dump more information when test fails.
diff --git a/test/e2e/e2e.go b/test/e2e/e2e.go
index 8d66bc5695a0..de1a45941932 100644
--- a/test/e2e/e2e.go
+++ b/test/e2e/e2e.go
@@ -50,6 +50,12 @@ func SetupAlternativeNamespace(t *testing.T) *test.Clients {
 	return SetupWithNamespace(t, test.AlternativeServingNamespace)
 }
 
+//SetupServingNamespaceforSecurityTesting creates the client objects needed in e2e tests
+// under the security testing namespace.
+func SetupServingNamespaceforSecurityTesting(t *testing.T) *test.Clients {
+	return SetupWithNamespace(t, test.ServingNamespaceforSecurityTesting)
+}
+
 // SetupWithNamespace creates the client objects needed in the e2e tests under the specified namespace.
 func SetupWithNamespace(t *testing.T, namespace string) *test.Clients {
 	pkgTest.SetupLoggingFlags()
diff --git a/test/e2e/probe_whitelist_test.go b/test/e2e/probe_whitelist_test.go
new file mode 100644
index 000000000000..2cbb26bbc7ef
--- /dev/null
+++ b/test/e2e/probe_whitelist_test.go
@@ -0,0 +1,93 @@
+// +build e2e
+
+/*
+Copyright 2020 The Knative Authors
+
+Licensed under the Apache License, Version 2.0 (the "License");
+you may not use this file except in compliance with the License.
+You may obtain a copy of the License at
+
+    http://www.apache.org/licenses/LICENSE-2.0
+
+Unless required by applicable law or agreed to in writing, software
+distributed under the License is distributed on an "AS IS" BASIS,
+WITHOUT WARRANTIES OR CONDITIONS OF ANY KIND, either express or implied.
+See the License for the specific language governing permissions and
+limitations under the License.
+*/
+
+package e2e
+
+import (
+	"net/http"
+	"testing"
+
+	metav1 "k8s.io/apimachinery/pkg/apis/meta/v1"
+	pkgTest "knative.dev/pkg/test"
+	"knative.dev/pkg/test/logstream"
+	"knative.dev/serving/test"
+	v1a1test "knative.dev/serving/test/v1alpha1"
+)
+
+//This test checks if the activator can probe
+//the service when istio end user auth policy is
+//applied on the service.
+//This test needs istio side car injected and
+//istio policy check enabled.
+//policy is present in test/config/security/policy.yaml
+//apply policy before running this test
+func TestProbeWhitelist(t *testing.T) {
+	t.Parallel()
+	cancel := logstream.Start(t)
+	defer cancel()
+
+	clients := SetupServingNamespaceforSecurityTesting(t)
+
+	names := test.ResourceNames{
+		Service: test.ObjectNameForTest(t),
+		Image:   "helloworld",
+	}
+
+	if test.ServingFlags.Https {
+		// Save the current Gateway to restore it after the test
+		oldGateway, err := clients.IstioClient.NetworkingV1alpha3().Gateways(v1a1test.Namespace).Get(v1a1test.GatewayName, metav1.GetOptions{})
+		if err != nil {
+			t.Fatalf("Failed to get Gateway %s/%s", v1a1test.Namespace, v1a1test.GatewayName)
+		}
+		test.CleanupOnInterrupt(func() { v1a1test.RestoreGateway(t, clients, *oldGateway) })
+		defer v1a1test.RestoreGateway(t, clients, *oldGateway)
+	}
+
+	test.CleanupOnInterrupt(func() { test.TearDown(clients, names) })
+	defer test.TearDown(clients, names)
+
+	t.Log("Creating a new Service")
+	resources, httpsTransportOption, err := v1a1test.CreateRunLatestServiceReady(t, clients, &names, test.ServingFlags.Https)
+	if err != nil {
+		t.Fatalf("Failed to create initial Service: %v: %v", names.Service, err)
+	}
+
+	url := resources.Route.Status.URL.URL()
+	var opt interface{}
+	if test.ServingFlags.Https {
+		url.Scheme = "https"
+		if httpsTransportOption == nil {
+			t.Fatalf("HTTPS transport option is nil")
+		}
+		opt = *httpsTransportOption
+	}
+	if _, err := pkgTest.WaitForEndpointState(
+		clients.KubeClient,
+		t.Logf,
+		url,
+		v1a1test.RetryingRouteInconsistency(pkgTest.MatchesAllOf(pkgTest.IsOneOfStatusCodes(http.StatusUnauthorized))),
+		"HelloWorldServesAuthFailed",
+		test.ServingFlags.ResolvableDomain,
+		opt); err != nil {
+		// check if side car is injected before reporting error
+		if _, err := getContainer(clients.KubeClient, resources.Service.Name, "istio-proxy", resources.Service.Namespace); err != nil {
+			t.Skip("Side car not enabled, skipping test")
+		}
+		t.Fatalf("The endpoint %s for Route %s didn't serve the expected status %d: %v", url, names.Route, http.StatusUnauthorized, err)
+	}
+}
diff --git a/test/e2e_flags.go b/test/e2e_flags.go
index f8f0bc640b82..07ae8d7937f3 100644
--- a/test/e2e_flags.go
+++ b/test/e2e_flags.go
@@ -33,6 +33,8 @@ const (
 	// namespace tests in.
 	AlternativeServingNamespace = "serving-tests-alt"
 
+	// ServingNamespaceforSecurityTesting is the namespace for security tests.
+	ServingNamespaceforSecurityTesting = "serving-tests-security"
 	// Environment propagation conformance test objects
 
 	// ConformanceConfigMap is the name of the configmap to propagate env variables from