On the question of predicting whether a carbon source can be utilized and how the concentration of ions in media files can be reproduced experimentally

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Hello, I still have a lot of questions I would like to ask you.
In the experiment, the bacteria will not grow without the addition of a carbon source, i.e. sugar (like glucose), but in the simulation, when I use the TSB medium provided and enter the command Gap filling when all the sugar has been removed, I get a growth result that the bacteria still grows, so the experiment does not grow and the simulation contradicts the simulation. How do I know if the bacteria are utilizing the carbon source?
In addition, the ions in the medium file such as K+, Mg2+, etc. are presented in the form of compounds in the experiment, so how should the concentration of these ions correspond to the compounds in the experiment?
Looking forward to your reply if you have time!