diff --git a/docs/rules/valid-mock-module-path.md b/docs/rules/valid-mock-module-path.md
index 9f5f8bdad..a3220dc8c 100644
--- a/docs/rules/valid-mock-module-path.md
+++ b/docs/rules/valid-mock-module-path.md
@@ -24,6 +24,9 @@ jest.mock('../../this/module/does/not/exist');
 
 // Local file that cannot be found
 jest.mock('../../this/path/does/not/exist.js');
+
+// Local file that cannot be found and is NOT virtual
+jest.mock('../../this/path/does/not/exist.js', undefined, { virtual: false });
 ```
 
 The following patterns are **not** considered errors:
@@ -38,6 +41,13 @@ jest.mock('../../this/module/really/does/exist');
 
 // Local file that cannot be found
 jest.mock('../../this/path/really/does/exist.js');
+
+// Module(s) that cannot be found but are configured as virtual
+jest.mock('@org/some-module-not-in-package-json', undefined, { virtual: true });
+jest.mock('some-module-not-in-package-json', undefined, { virtual: true });
+
+// Local file that cannot be found but is configured as virtual
+jest.mock('../../this/path/does/not/exist.js', undefined, { virtual: true });
 ```
 
 ## Options
diff --git a/src/rules/__tests__/valid-mock-module-path.test.ts b/src/rules/__tests__/valid-mock-module-path.test.ts
index 8151a9961..48e82d876 100644
--- a/src/rules/__tests__/valid-mock-module-path.test.ts
+++ b/src/rules/__tests__/valid-mock-module-path.test.ts
@@ -10,7 +10,7 @@ import {
 const ruleTester = new RuleTester({
   parser: espreeParser,
   parserOptions: {
-    ecmaVersion: 2015,
+    ecmaVersion: 2018,
   },
 });
 
@@ -62,6 +62,52 @@ ruleTester.run('valid-mock-module-path', rule, {
       code: 'jest.mock("./fixtures/module/bar")',
       options: [{ moduleFileExtensions: ['.css'] }],
     },
+    {
+      filename: __filename,
+      code: 'jest.mock("./fixtures/module/tsx/foo", undefined, { virtual: false })',
+    },
+    {
+      filename: __filename,
+      code: 'jest.doMock("./fixtures/module/tsx/foo", undefined, { virtual: false })',
+    },
+    {
+      filename: __filename,
+      code: 'jest.doMock("./fixtures/module/tsx/foo", undefined, { ...{} })',
+    },
+    {
+      filename: __filename,
+      code: 'jest.doMock("./fixtures/module/tsx/foo", undefined, { virtual: [] })',
+    },
+    {
+      filename: __filename,
+      code: 'jest.mock("../module/does/not/exist", undefined, { virtual: true })',
+    },
+    {
+      filename: __filename,
+      code: 'jest.doMock("../module/does/not/exist", undefined, { virtual: true })',
+    },
+    {
+      filename: __filename,
+      code: 'jest.doMock("../module/does/not/exist", undefined, { "virtual": true })',
+    },
+    {
+      filename: __filename,
+      code: 'jest.doMock("../module/does/not/exist", undefined, { ["virtual"]: true })',
+    },
+    {
+      filename: __filename,
+      code: 'jest.doMock("../module/does/not/exist", undefined, { [`virtual`]: true })',
+    },
+    {
+      // jest only cares if the value is truthy...
+      filename: __filename,
+      code: 'jest.doMock("../module/does/not/exist", undefined, { virtual: 1 })',
+    },
+    {
+      // jest only cares if the value is truthy...
+      filename: __filename,
+      code: 'jest.doMock("../module/does/not/exist", undefined, { virtual: "yes" })',
+    },
   ],
   invalid: [
     {
@@ -154,6 +200,127 @@ ruleTester.run('valid-mock-module-path', rule, {
         },
       ],
     },
+    {
+      filename: __filename,
+      code: "jest.mock('../module/does/not/exist', undefined, {})",
+      errors: [
+        {
+          messageId: 'invalidMockModulePath',
+          data: { moduleName: "'../module/does/not/exist'" },
+          column: 1,
+          line: 1,
+        },
+      ],
+    },
+    {
+      filename: __filename,
+      code: "jest.mock('../module/does/not/exist', undefined, { assumeExists: true })",
+      errors: [
+        {
+          messageId: 'invalidMockModulePath',
+          data: { moduleName: "'../module/does/not/exist'" },
+          column: 1,
+          line: 1,
+        },
+      ],
+    },
+    {
+      filename: __filename,
+      code: "jest.mock('../module/does/not/exist', undefined, { virtual: false })",
+      errors: [
+        {
+          messageId: 'invalidMockModulePath',
+          data: { moduleName: "'../module/does/not/exist'" },
+          column: 1,
+          line: 1,
+        },
+      ],
+    },
+    {
+      filename: __filename,
+      code: "jest.doMock('../module/does/not/exist', undefined, { virtual: false })",
+      errors: [
+        {
+          messageId: 'invalidMockModulePath',
+          data: { moduleName: "'../module/does/not/exist'" },
+          column: 1,
+          line: 1,
+        },
+      ],
+    },
+    {
+      filename: __filename,
+      code: "jest.doMock('../module/does/not/exist', undefined, { virtual: 0 })",
+      errors: [
+        {
+          messageId: 'invalidMockModulePath',
+          data: { moduleName: "'../module/does/not/exist'" },
+          column: 1,
+          line: 1,
+        },
+      ],
+    },
+    {
+      filename: __filename,
+      code: dedent`
+        const virtual = false;
+
+        jest.doMock('../module/does/not/exist', undefined, { virtual })
+      `,
+      errors: [
+        {
+          messageId: 'invalidMockModulePath',
+          data: { moduleName: "'../module/does/not/exist'" },
+          column: 1,
+          line: 3,
+        },
+      ],
+    },
+    {
+      filename: __filename,
+      code: dedent`
+        const virtual = true;
+
+        jest.doMock('../module/does/not/exist', undefined, { virtual })
+      `,
+      errors: [
+        {
+          messageId: 'invalidMockModulePath',
+          data: { moduleName: "'../module/does/not/exist'" },
+          column: 1,
+          line: 3,
+        },
+      ],
+    },
+    {
+      filename: __filename,
+      code: dedent`
+        const prop = 'virtual';
+
+        jest.doMock('../module/does/not/exist', undefined, { [prop]: true })
+      `,
+      errors: [
+        {
+          messageId: 'invalidMockModulePath',
+          data: { moduleName: "'../module/does/not/exist'" },
+          column: 1,
+          line: 3,
+        },
+      ],
+    },
+    {
+      // we don't attempt to resolve the result of object spreads
+      filename: __filename,
+      code: "jest.doMock('../module/does/not/exist', undefined, { ...{ virtual: true } })",
+      errors: [
+        {
+          messageId: 'invalidMockModulePath',
+          data: { moduleName: "'../module/does/not/exist'" },
+          column: 1,
+          line: 1,
+        },
+      ],
+    },
   ],
 });
 
diff --git a/src/rules/valid-mock-module-path.ts b/src/rules/valid-mock-module-path.ts
index 3954f4db2..0a59d9ab6 100644
--- a/src/rules/valid-mock-module-path.ts
+++ b/src/rules/valid-mock-module-path.ts
@@ -73,6 +73,28 @@ export default createRule<
           return;
         }
 
+        if (node.arguments[2]?.type === AST_NODE_TYPES.ObjectExpression) {
+          const hasTrueVirtualProperty = node.arguments[2].properties.some(
+            expression => {
+              if (expression.type === AST_NODE_TYPES.Property) {
+                const { key, value } = expression;
+
+                return (
+                  isSupportedAccessor(key, 'virtual') &&
+                  value.type === AST_NODE_TYPES.Literal &&
+                  value.value
+                );
+              }
+
+              return false;
+            },
+          );
+
+          if (hasTrueVirtualProperty) {
+            return;
+          }
+        }
+
         try {
           if (!moduleName.value.startsWith('.')) {
             require.resolve(moduleName.value);