From d19d969cb8e4a29f0c988f5e4ec48bf117f2ea4c Mon Sep 17 00:00:00 2001 From: Ivan Pan Date: Fri, 14 Jul 2017 15:34:28 -0700 Subject: [PATCH] Torrent Suite Software 5.4.0 Release --- Analysis/AnalysisOrg/ClonalFilter.cpp | 2 +- .../AnalysisOrg/IO/BeadfindControlOpts.cpp | 155 +- Analysis/AnalysisOrg/IO/BeadfindControlOpts.h | 3 + Analysis/AnalysisOrg/IO/BkgControlOpts.cpp | 46 +- Analysis/AnalysisOrg/IO/BkgControlOpts.h | 4 +- Analysis/AnalysisOrg/IO/CommandLineOpts.cpp | 41 +- Analysis/AnalysisOrg/IO/FlowContext.cpp | 10 +- Analysis/AnalysisOrg/IO/ProgramState.cpp | 1 + Analysis/AnalysisOrg/ImageLoaderQueue.cpp | 5 +- Analysis/AnalysisOrg/MaskFunctions.cpp | 246 +- Analysis/AnalysisOrg/MaskFunctions.h | 3 +- Analysis/AnalysisOrg/ProcessImageToWell.cpp | 4 +- Analysis/AnalysisOrg/SpatialContext.cpp | 2 + Analysis/AnalysisOrg/SpatialContext.h | 1 + Analysis/AnalysisOrg/cudaWrapper.cpp | 5 +- .../justBeadFind/SeparatorInterface.cpp | 6 +- Analysis/BaseCaller/BarcodeClassifier.cpp | 121 +- 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tsconfig/apt-mirror/link-test-mirror.sh create mode 100644 tsconfig/apt-mirror/mirror.list create mode 100644 tsconfig/apt-mirror/test-mirror.list delete mode 100755 tsconfig/bin/TSconfig-ansible delete mode 100755 tsconfig/bin/TSconfig-legacy delete mode 100755 tsconfig/bin/TScreateLocalRepository delete mode 100755 tsconfig/bin/TSstaticip-ansible delete mode 100755 tsconfig/bin/TSstaticip-legacy mode change 100644 => 100755 tsconfig/ion_tsconfig/TSconfig.py create mode 100644 tsconfig/rnd_playbooks/README.md create mode 100644 tsconfig/rnd_playbooks/open_iptables.j2 create mode 100644 tsconfig/rnd_playbooks/open_iptables.yml rename tsconfig/{ansible => rnd_playbooks}/sentry.yml (91%) create mode 100644 tsconfig/rnd_playbooks/sshd_keyonly.yml create mode 100644 tsconfig/rnd_playbooks/torrentpy_database.yml create mode 100644 tsvm/tsvm-enable-nfs-rule mode change 100644 => 100755 tsvm/tsvm-include diff --git a/Analysis/AnalysisOrg/ClonalFilter.cpp b/Analysis/AnalysisOrg/ClonalFilter.cpp index c8bedfc9..cc679d77 100644 --- a/Analysis/AnalysisOrg/ClonalFilter.cpp +++ b/Analysis/AnalysisOrg/ClonalFilter.cpp @@ -1,6 +1,6 @@ /* Copyright (C) 2012 Ion Torrent Systems, Inc. All Rights Reserved */ #include "ClonalFilter.h" -#include "mixed.h" +#include "ClonalFilter/mixed.h" #include "hdf5.h" using namespace std; diff --git a/Analysis/AnalysisOrg/IO/BeadfindControlOpts.cpp b/Analysis/AnalysisOrg/IO/BeadfindControlOpts.cpp index 617bb9cd..b9e438a0 100644 --- a/Analysis/AnalysisOrg/IO/BeadfindControlOpts.cpp +++ b/Analysis/AnalysisOrg/IO/BeadfindControlOpts.cpp @@ -88,6 +88,9 @@ void BeadfindControlOpts::PrintHelp() printf (" --beadfind-zero-flows STRING beadfind double tap flows []\n"); printf (" --beadfind-num-threads INT beadfind number of threads [-1]\n"); printf (" --beadfind-mesh-step VECTOR_INT beadfind mesh steps for differntial separator [100,100]\n"); + printf (" --beadfind-acq-threshold VECTOR_INT beadfind threshold for hinge model for acquisition flow [-10,12,5,500]\n"); + printf (" --beadfind-bf-threshold VECTOR_INT beadfind threshold for hinge model for beadfind flow [-5,300,-20000,-10]\n"); + printf (" --exclusion-mask FILE exclusion mask file name []\n"); printf (" --bfold BOOL BF_ADVANCED [true]\n"); printf (" --noduds BOOL noduds [false]\n"); printf ("\n"); @@ -95,79 +98,93 @@ void BeadfindControlOpts::PrintHelp() void BeadfindControlOpts::SetOpts(OptArgs &opts, Json::Value& json_params) { - beadfindType = RetrieveParameterString(opts, json_params, '-', "beadfind-type", "differential"); - if ( beadfindType != "differential" ) - { - fprintf ( stderr, "*Error* - Illegal option to --beadfind-type: %s, valid options are 'differential'\n", - beadfindType.c_str() ); - exit ( EXIT_FAILURE ); - } - string s1 = RetrieveParameterString(opts, json_params, '-', "use-beadmask", ""); - if(s1.length() > 0) - { - sprintf(beadMaskFile, "%s", s1.c_str()); - } - maskFileCategorized = RetrieveParameterBool(opts, json_params, '-', "beadmask-categorized", false); - string s2 = RetrieveParameterString(opts, json_params, '-', "beadfind-basis", "naoh"); - if(s2.length() > 0) - { - bfType = s2; - if ( bfType != "naoh" && bfType != "positive" && bfType != "nobuffer") - { - fprintf ( stderr, "*Error* - Illegal option to --beadfind-basis: %s, valid options are 'naoh','nobuffer' or 'positive'\n", bfType.c_str() ); - exit ( EXIT_FAILURE ); - } - } - bfDat = RetrieveParameterString(opts, json_params, '-', "beadfind-dat", "beadfind_pre_0003.dat"); - bfBgDat = RetrieveParameterString(opts, json_params, '-', "beadfind-bgdat", ""); - sdAsBf = RetrieveParameterBool(opts, json_params, '-', "beadfind-sdasbf", true); - bfMult = RetrieveParameterFloat(opts, json_params, '-', "beadfind-bfmult", 1.0); - bfMinLiveRatio = RetrieveParameterDouble(opts, json_params, '-', "beadfind-minlive", 0.0001); - bfMinLiveLibSnr = RetrieveParameterDouble(opts, json_params, '-', "beadfind-minlivesnr", 4.0); + beadfindType = RetrieveParameterString(opts, json_params, '-', "beadfind-type", "differential"); + if ( beadfindType != "differential" ) + { + fprintf ( stderr, "*Error* - Illegal option to --beadfind-type: %s, valid options are 'differential'\n", + beadfindType.c_str() ); + exit ( EXIT_FAILURE ); + } + string s1 = RetrieveParameterString(opts, json_params, '-', "use-beadmask", ""); + if(s1.length() > 0) + { + sprintf(beadMaskFile, "%s", s1.c_str()); + } + maskFileCategorized = RetrieveParameterBool(opts, json_params, '-', "beadmask-categorized", false); + + exclusionMaskFile = RetrieveParameterString(opts, json_params, '-', "exclusion-mask", ""); + + string s2 = RetrieveParameterString(opts, json_params, '-', "beadfind-basis", "naoh"); + if(s2.length() > 0) + { + bfType = s2; + if ( bfType != "naoh" && bfType != "positive" && bfType != "nobuffer") + { + fprintf ( stderr, "*Error* - Illegal option to --beadfind-basis: %s, valid options are 'naoh','nobuffer' or 'positive'\n", bfType.c_str() ); + exit ( EXIT_FAILURE ); + } + } + bfDat = RetrieveParameterString(opts, json_params, '-', "beadfind-dat", "beadfind_pre_0003.dat"); + bfBgDat = RetrieveParameterString(opts, json_params, '-', "beadfind-bgdat", ""); + sdAsBf = RetrieveParameterBool(opts, json_params, '-', "beadfind-sdasbf", true); + bfMult = RetrieveParameterFloat(opts, json_params, '-', "beadfind-bfmult", 1.0); + bfMinLiveRatio = RetrieveParameterDouble(opts, json_params, '-', "beadfind-minlive", 0.0001); + bfMinLiveLibSnr = RetrieveParameterDouble(opts, json_params, '-', "beadfind-minlivesnr", 4.0); bfMinLiveTfSnr = RetrieveParameterDouble(opts, json_params, '-', "beadfind-min-tf-snr", 7); - minTfPeakMax = RetrieveParameterFloat(opts, json_params, '-', "beadfind-tf-min-peak", 40.0); - minLibPeakMax = RetrieveParameterFloat(opts, json_params, '-', "beadfind-lib-min-peak", 10.0); - bfLibFilterQuantile = RetrieveParameterDouble(opts, json_params, '-', "beadfind-lib-filt", 1.0); - bfTfFilterQuantile = RetrieveParameterDouble(opts, json_params, '-', "beadfind-tf-filt", 1.0); - skipBeadfindSdRecover = RetrieveParameterInt(opts, json_params, '-', "beadfind-skip-sd-recover", 1); + minTfPeakMax = RetrieveParameterFloat(opts, json_params, '-', "beadfind-tf-min-peak", 40.0); + minLibPeakMax = RetrieveParameterFloat(opts, json_params, '-', "beadfind-lib-min-peak", 10.0); + bfLibFilterQuantile = RetrieveParameterDouble(opts, json_params, '-', "beadfind-lib-filt", 1.0); + bfTfFilterQuantile = RetrieveParameterDouble(opts, json_params, '-', "beadfind-tf-filt", 1.0); + skipBeadfindSdRecover = RetrieveParameterInt(opts, json_params, '-', "beadfind-skip-sd-recover", 1); filterNoisyCols = RetrieveParameterString(opts, json_params, '-', "beadfind-filt-noisy-col", "none"); - beadfindUseSepRef = RetrieveParameterBool(opts, json_params, '-', "beadfind-sep-ref", false); - beadfindSmoothTrace = RetrieveParameterBool(opts, json_params, '-', "beadfind-smooth-trace", false); + beadfindUseSepRef = RetrieveParameterBool(opts, json_params, '-', "beadfind-sep-ref", false); + beadfindSmoothTrace = RetrieveParameterBool(opts, json_params, '-', "beadfind-smooth-trace", false); bfOutputDebug = RetrieveParameterInt(opts, json_params, '-', "beadfind-diagnostics", 2); - bool b1 = RetrieveParameterBool(opts, json_params, '-', "bead-washout", false); - SINGLEBF = !b1; - useBeadfindGainCorrection = RetrieveParameterBool(opts, json_params, '-', "beadfind-gain-correction", true); - useDatacollectGainCorrectionFile = RetrieveParameterBool(opts, json_params, '-', "datacollect-gain-correction", false); - blobFilter = RetrieveParameterBool(opts, json_params, '-', "beadfind-blob-filter", false); - predictFlowStart = RetrieveParameterInt(opts, json_params, '-', "beadfind-predict-start", -1); - predictFlowEnd = RetrieveParameterInt(opts, json_params, '-', "beadfind-predict-end", -1); - useSignalReference = RetrieveParameterInt(opts, json_params, '-', "beadfind-sig-ref-type", -1); - if(useSignalReference == -1) - { - useSignalReference = 4; - } - else - { - useSignalReferenceSet = true; - } - doubleTapFlows = RetrieveParameterString(opts, json_params, '-', "beadfind-zero-flows", ""); - numThreads = RetrieveParameterInt(opts, json_params, '-', "beadfind-num-threads", -1); + bool b1 = RetrieveParameterBool(opts, json_params, '-', "bead-washout", false); + SINGLEBF = !b1; + useBeadfindGainCorrection = RetrieveParameterBool(opts, json_params, '-', "beadfind-gain-correction", true); + useDatacollectGainCorrectionFile = RetrieveParameterBool(opts, json_params, '-', "datacollect-gain-correction", false); + blobFilter = RetrieveParameterBool(opts, json_params, '-', "beadfind-blob-filter", false); + predictFlowStart = RetrieveParameterInt(opts, json_params, '-', "beadfind-predict-start", -1); + predictFlowEnd = RetrieveParameterInt(opts, json_params, '-', "beadfind-predict-end", -1); + useSignalReference = RetrieveParameterInt(opts, json_params, '-', "beadfind-sig-ref-type", -1); + if(useSignalReference == -1) + { + useSignalReference = 4; + } + else + { + useSignalReferenceSet = true; + } + doubleTapFlows = RetrieveParameterString(opts, json_params, '-', "beadfind-zero-flows", ""); + numThreads = RetrieveParameterInt(opts, json_params, '-', "beadfind-num-threads", -1); - //jz the following comes from CommandLineOpts::GetOpts - BF_ADVANCED = RetrieveParameterBool(opts, json_params, '-', "bfold", true); - noduds = RetrieveParameterBool(opts, json_params, '-', "noduds", false); - string sb = RetrieveParameterString(opts, json_params, 'b', "beadfindfile", ""); - if(sb.length() > 0) - { - snprintf ( preRunbfFileBase, 256, "%s", sb.c_str() ); + //jz the following comes from CommandLineOpts::GetOpts + BF_ADVANCED = RetrieveParameterBool(opts, json_params, '-', "bfold", true); + noduds = RetrieveParameterBool(opts, json_params, '-', "noduds", false); + string sb = RetrieveParameterString(opts, json_params, 'b', "beadfindfile", ""); + if(sb.length() > 0) + { + snprintf ( preRunbfFileBase, 256, "%s", sb.c_str() ); bfFileBase[0] = '\0'; SINGLEBF = true; - } - vector vec; - RetrieveParameterVectorInt(opts, json_params, '-', "beadfind-mesh-step", "", vec); - if(vec.size() == 2) - { - meshStepX = vec[0]; - meshStepY = vec[1]; - } + } + vector vec; + RetrieveParameterVectorInt(opts, json_params, '-', "beadfind-mesh-step", "", vec); + if(vec.size() == 2) + { + meshStepX = vec[0]; + meshStepY = vec[1]; + } + + RetrieveParameterVectorFloat(opts, json_params, '-', "beadfind-acq-threshold","-10,3,5,500", beadfindAcqThreshold); + if( (beadfindAcqThreshold.size() != 4) || (beadfindAcqThreshold[0] > beadfindAcqThreshold[1]) || (beadfindAcqThreshold[2] > beadfindAcqThreshold[3]) ){ + fprintf ( stderr, "*Error* - Illegal option value --beadfind-acq-threshold. Provide 4 values minFirstSlope,maxFirstSlope,minSecondSlope,maxSecondSlope. \n"); + exit ( EXIT_FAILURE ); + } + RetrieveParameterVectorFloat(opts, json_params, '-', "beadfind-bf-threshold","-5,300,-2000,-10", beadfindBfThreshold); + if( (beadfindBfThreshold.size() != 4) || (beadfindBfThreshold[0] > beadfindBfThreshold[1]) || (beadfindBfThreshold[2] > beadfindBfThreshold[3]) ){ + fprintf ( stderr, "*Error* - Illegal option value --beadfind-bf-threshold. Provide 4 values minFirstSlope,maxFirstSlope,minSecondSlope,maxSecondSlope. \n"); + exit ( EXIT_FAILURE ); + } } diff --git a/Analysis/AnalysisOrg/IO/BeadfindControlOpts.h b/Analysis/AnalysisOrg/IO/BeadfindControlOpts.h index 7bedce7a..4b787bf5 100644 --- a/Analysis/AnalysisOrg/IO/BeadfindControlOpts.h +++ b/Analysis/AnalysisOrg/IO/BeadfindControlOpts.h @@ -22,6 +22,7 @@ class BeadfindControlOpts{ bool beadfindSmoothTrace; std::string filterNoisyCols; char *beadMaskFile; + std::string exclusionMaskFile; bool maskFileCategorized; char bfFileBase[MAX_PATH_LENGTH]; char preRunbfFileBase[MAX_PATH_LENGTH]; @@ -49,6 +50,8 @@ class BeadfindControlOpts{ int predictFlowEnd; int meshStepX; int meshStepY; + std::vector beadfindAcqThreshold; + std::vector beadfindBfThreshold; void DefaultBeadfindControl(); void PrintHelp(); void SetOpts(OptArgs &opts, Json::Value& json_params); diff --git a/Analysis/AnalysisOrg/IO/BkgControlOpts.cpp b/Analysis/AnalysisOrg/IO/BkgControlOpts.cpp index 9da9281d..8af74de4 100644 --- a/Analysis/AnalysisOrg/IO/BkgControlOpts.cpp +++ b/Analysis/AnalysisOrg/IO/BkgControlOpts.cpp @@ -156,76 +156,40 @@ void TraceControl::SetOpts(OptArgs &opts, Json::Value& json_params) void BkgModelControlOpts::PrintHelp() { printf (" BkgModelControlOpts\n"); - printf (" --mixed-first-flow INT mixed first flow of polyclonal filter [12]\n"); - printf (" --mixed-last-flow INT mixed last flow of polyclonal filter [72]\n"); - printf (" --max-iterations INT max iterations of polyclonal filter [30]\n"); - printf (" --mixed-model-option INT mixed model option of polyclonal filter [0]\n"); - printf (" --mixed-stringency DOUBLE mixed stringency of polyclonal filter [0.5]\n"); printf (" --nokey BOOL nokey [false]\n"); printf (" --xtalk-correction BOOL enable trace xtalk correction [false for Proton; true for P-zero and PGM]\n"); printf (" --n-unfiltered-lib INT number of unfiltered library random samples [100000]\n"); printf (" --bkg-dont-emphasize-by-compression INT emphasize by compression [1]\n"); - printf (" --clonal-filter-bkgmodel BOOL enable polyclonal filter [false for Proton; true for PGM]\n"); - printf (" --clonal-filter-debug BOOL enable polyclonal filter debug output \n"); - printf (" --clonal-filter-use-last-iter-params BOOL use last EM iteration cluster parameters if no convergence \n"); - printf (" --filter-extreme-ppf-only BOOL Skip polyclonal filter training and filter for extreme ppf only \n"); printf (" --sigproc-regional-smoothing-alpha FLOAT sigproc regional smoothing alpha [1.0]\n"); printf (" --sigproc-regional-smoothing-gamma FLOAT sigproc regional smoothing gamma [1.0]\n"); printf (" --restart-reg-params-file STRING json file to input/output regional parameters\n"); printf ("\n"); + polyclonal_filter.PrintHelp(true); signal_chunks.PrintHelp(); trace_control.PrintHelp(); pest_control.PrintHelp(); gpuControl.PrintHelp(); } -void BkgModelControlOpts::SetOpts(OptArgs &opts, Json::Value& json_params) +void BkgModelControlOpts::SetOpts(OptArgs &opts, Json::Value& json_params, int num_flows) { gpuControl.SetOpts(opts, json_params); signal_chunks.SetOpts(opts, json_params); pest_control.SetOpts(opts, json_params); trace_control.SetOpts(opts, json_params); + polyclonal_filter.SetOpts(true, opts,json_params, num_flows); + //jz the following comes from CommandLineOpts::GetOpts unfiltered_library_random_sample = RetrieveParameterInt(opts, json_params, '-', "n-unfiltered-lib", 100000); enable_trace_xtalk_correction = RetrieveParameterBool(opts, json_params, '-', "xtalk-correction", true); emphasize_by_compression = RetrieveParameterInt(opts, json_params, '-', "bkg-dont-emphasize-by-compression", 1); nokey = RetrieveParameterBool(opts, json_params, '-', "nokey", false); + washout_threshold = RetrieveParameterFloat(opts, json_params, '-', "bkg-washout-threshold", WASHOUT_THRESHOLD); washout_flow_detection = RetrieveParameterInt(opts, json_params, '-', "bkg-washout-flow-detection", WASHOUT_FLOW_DETECTION); - polyclonal_filter.enable = RetrieveParameterBool(opts, json_params, '-', "clonal-filter-bkgmodel", true); - polyclonal_filter.mixed_first_flow = RetrieveParameterInt(opts, json_params, '-', "mixed-first-flow", 12); - polyclonal_filter.mixed_last_flow = RetrieveParameterInt(opts, json_params, '-', "mixed-last-flow", 72); - polyclonal_filter.max_iterations = RetrieveParameterInt(opts, json_params, '-', "max-iterations", 30); - polyclonal_filter.mixed_model_option = RetrieveParameterInt(opts, json_params, '-', "mixed-model-option", 0); - polyclonal_filter.verbose = RetrieveParameterBool(opts, json_params, '-', "clonal-filter-debug", false); - polyclonal_filter.use_last_iter_params = RetrieveParameterBool(opts, json_params, '-', "clonal-filter-use-last-iter-params", true); - polyclonal_filter.filter_extreme_ppf_only = RetrieveParameterBool(opts, json_params, '-', "filter-extreme-ppf-only", false); - double stringency = RetrieveParameterDouble(opts, json_params, '-', "mixed-stringency", 0.5); - if(stringency < 0) - { - stringency = 0; - } - if(stringency > 1) - { - stringency = 1; - } - //transform it to log scale - if(stringency > 0.5) - { - polyclonal_filter.mixed_stringency = 0.5 * std::log10((stringency - 0.5)*18 + 1) + 0.5; - } - else if (stringency< 0.5) - { - polyclonal_filter.mixed_stringency = 0.5 - 0.5 * std::log10(( 0.5 - stringency)*18 + 1); - } - else - { - polyclonal_filter.mixed_stringency = 0.5; - } - regional_smoothing.alpha = RetrieveParameterFloat(opts, json_params, '-', "sigproc-regional-smoothing-alpha", 1.0f); regional_smoothing.gamma = RetrieveParameterFloat(opts, json_params, '-', "sigproc-regional-smoothing-gamma", 1.0f); diff --git a/Analysis/AnalysisOrg/IO/BkgControlOpts.h b/Analysis/AnalysisOrg/IO/BkgControlOpts.h index 992583c0..a74292b5 100644 --- a/Analysis/AnalysisOrg/IO/BkgControlOpts.h +++ b/Analysis/AnalysisOrg/IO/BkgControlOpts.h @@ -20,7 +20,7 @@ #include "SeqList.h" #include "GpuControlOpts.h" #include "DebugMe.h" -#include "polyclonal_filter.h" +#include "ClonalFilter/polyclonal_filter.h" #include "FlowSequence.h" #include "OptBase.h" @@ -94,7 +94,7 @@ class BkgModelControlOpts{ void DefaultBkgModelControl(void); void PrintHelp(); - void SetOpts(OptArgs &opts, Json::Value& json_params); + void SetOpts(OptArgs &opts, Json::Value& json_params, int num_flows); }; #endif // BKGCONTROLOPTS_H diff --git a/Analysis/AnalysisOrg/IO/CommandLineOpts.cpp b/Analysis/AnalysisOrg/IO/CommandLineOpts.cpp index d97e43b2..292e6dd2 100644 --- a/Analysis/AnalysisOrg/IO/CommandLineOpts.cpp +++ b/Analysis/AnalysisOrg/IO/CommandLineOpts.cpp @@ -13,7 +13,7 @@ #include "CommandLineOpts.h" #include "ChipIdDecoder.h" #include "IonErr.h" -#include "mixed.h" +#include "ClonalFilter/mixed.h" using namespace std; @@ -93,22 +93,23 @@ void CommandLineOpts::PrintHelp() void CommandLineOpts::SetOpts(OptArgs &opts, Json::Value& json_params) { - bkg_control.DefaultBkgModelControl(); - bkg_control.SetOpts(opts, json_params); - bfd_control.DefaultBeadfindControl(); - bfd_control.SetOpts(opts, json_params); - img_control.DefaultImageOpts(); - img_control.SetOpts(opts, json_params); - mod_control.SetOpts(opts, json_params); - loc_context.DefaultSpatialContext(); - loc_context.SetOpts(opts, json_params); - flow_context.DefaultFlowFormula(); - flow_context.SetOpts(opts, json_params); - key_context.DefaultKeys(); - key_context.SetOpts(opts, json_params); - no_control.SetOpts(opts, json_params); - sys_context.DefaultSystemContext(); - sys_context.SetOpts(opts, json_params); + flow_context.DefaultFlowFormula(); + flow_context.SetOpts(opts, json_params); + bkg_control.DefaultBkgModelControl(); + // Apparently flow_context does not have a member that gives me the total number of flows in a run, so I'm hard coding this baby! + bkg_control.SetOpts(opts, json_params, 100000); + bfd_control.DefaultBeadfindControl(); + bfd_control.SetOpts(opts, json_params); + img_control.DefaultImageOpts(); + img_control.SetOpts(opts, json_params); + mod_control.SetOpts(opts, json_params); + loc_context.DefaultSpatialContext(); + loc_context.SetOpts(opts, json_params); + key_context.DefaultKeys(); + key_context.SetOpts(opts, json_params); + no_control.SetOpts(opts, json_params); + sys_context.DefaultSystemContext(); + sys_context.SetOpts(opts, json_params); // We can only do save and restore on an even flow block boundary. // Now that all the parameters have been set, we can check their sanity. @@ -226,6 +227,7 @@ ValidateOpts::ValidateOpts() // BeadfindControlOpts m_opts["beadfind-type"] = VT_STRING; m_opts["use-beadmask"] = VT_STRING; + m_opts["exclusion-mask"] = VT_STRING; m_opts["beadmask-categorized"] = VT_BOOL; m_opts["beadfind-basis"] = VT_STRING; m_opts["beadfind-dat"] = VT_STRING; @@ -437,6 +439,11 @@ void ValidateOpts::Validate(const int argc, char *argv[]) { if((!isdigit(argv[i][1])) && (argv[i][1] != '.')) { + if(s.length() > 2 && argv[i][1] != '-' && argv[i][2] != ' ' && argv[i][2] != '=') // handle mis-typing long option to short option + { + fprintf ( stdout, "WARNING: %s may miss a leading - . Please check if it is a long option.\n", s.c_str()); + } + s = s.substr(1, s.length() - 1); if(argv[i][1] == '-') // long option { diff --git a/Analysis/AnalysisOrg/IO/FlowContext.cpp b/Analysis/AnalysisOrg/IO/FlowContext.cpp index ea4dc0b9..99b968ff 100644 --- a/Analysis/AnalysisOrg/IO/FlowContext.cpp +++ b/Analysis/AnalysisOrg/IO/FlowContext.cpp @@ -36,9 +36,11 @@ void FlowContext::DetectFlowFormula ( char *explog_path ) // @TODO: obviously needs to be refactored into flow routine // expand flow formula = flowOrder into appropriate number of flows //Determine total number of flows in experiment or previous analysis - if ( true ) { - numTotalFlows = GetTotalFlows (explog_path ); - assert ( numTotalFlows > 0 ); + numTotalFlows = GetTotalFlows (explog_path ); + //assert ( numTotalFlows > 0 ); //TS-14040: changed assert to error log and exit + if ( numTotalFlows <= 0 ) { + fprintf ( stderr, "DetectFlowFormula Error: numTotalFlows=%d<=0\n",numTotalFlows); + exit ( EXIT_FAILURE ); } //If flow order was not specified on command line, @@ -47,12 +49,10 @@ void FlowContext::DetectFlowFormula ( char *explog_path ) if ( flowOrder ) free ( flowOrder ); // Get flow order from the explog.txt file - if ( true ) { flowOrder = GetPGMFlowOrder ( explog_path ); assert ( flowOrder != NULL ); numFlowsPerCycle = strlen ( flowOrder ); assert ( numFlowsPerCycle > 0 ); - } } // Adjust number of flows according to any command line options which may have been used diff --git a/Analysis/AnalysisOrg/IO/ProgramState.cpp b/Analysis/AnalysisOrg/IO/ProgramState.cpp index 99b55182..c026d8c4 100644 --- a/Analysis/AnalysisOrg/IO/ProgramState.cpp +++ b/Analysis/AnalysisOrg/IO/ProgramState.cpp @@ -337,6 +337,7 @@ void ProgramState::SetLocContext(Json::Value &json, SpatialContext &loc_context) loc_context.cropRegions[r].w = json["cropRegions"]["w"][r].asInt(); loc_context.cropRegions[r].h = json["cropRegions"]["h"][r].asInt(); loc_context.cropRegions[r].index = json["cropRegions"]["index"][r].asInt(); + loc_context.isCropped = true; } loc_context.cropped_region_x_offset = json["cropped_region_x_offset"].asInt(); diff --git a/Analysis/AnalysisOrg/ImageLoaderQueue.cpp b/Analysis/AnalysisOrg/ImageLoaderQueue.cpp index ca58713f..b4e77f7c 100644 --- a/Analysis/AnalysisOrg/ImageLoaderQueue.cpp +++ b/Analysis/AnalysisOrg/ImageLoaderQueue.cpp @@ -194,7 +194,10 @@ void *FileLoadWorker ( void *arg ) int buffer_ix = one_img_loader->cur_buffer; // setting the mean of frames to zero will be done by the bkgmodel as soon as its loaded. - img->SetMeanOfFramesToZero ( one_img_loader->normStart, one_img_loader->normEnd,0 ); + +// the traces will be zero'd by the bknd model loader anyway, no need to do them here. + // img->SetMeanOfFramesToZero ( one_img_loader->normStart, one_img_loader->normEnd,0 ); + // calculate the smooth pH step amplitude in empty wells across the whole image if ( one_img_loader->doEmptyWellNormalization ) diff --git a/Analysis/AnalysisOrg/MaskFunctions.cpp b/Analysis/AnalysisOrg/MaskFunctions.cpp index 98d493d4..2dda89bb 100644 --- a/Analysis/AnalysisOrg/MaskFunctions.cpp +++ b/Analysis/AnalysisOrg/MaskFunctions.cpp @@ -1,190 +1,90 @@ /* Copyright (C) 2010 Ion Torrent Systems, Inc. All Rights Reserved */ #include "MaskFunctions.h" +void SetExcludeMask(SpatialContext& loc_context, Mask* maskPtr, char* chipType, + int rows, int cols, std::string exclusionMaskFile, + bool isThumbnail) +{ + bool applyExclusionMask = (isThumbnail) ? false : true; + + /* + * If we get a cropped region definition from the command line, we want the + * whole chip to be MaskExclude + * except for the defined crop region(s) which are marked MaskEmpty. If no + * cropRegion defined on command line, + * then we proceed with marking the entire chip MaskEmpty + * + */ + + if ( loc_context.isCropped ) { + maskPtr->Init(cols, rows, MaskExclude); + } else { + maskPtr->Init(cols, rows, MaskEmpty); + } + if (applyExclusionMask && !exclusionMaskFile.empty()) { -//@TODO: Please do not use inline #ifdefs to rewrite code -// this should only be done >at the function level< to maintain readability. -// if you believe the function is too long to do this, >then rewrite the function to be shorter or more than one piece<. + // apply one or more cropRegions, mark them MaskEmpty + for (int q = 0; q < loc_context.numCropRegions; q++) { + maskPtr->MarkRegion(loc_context.cropRegions[q], MaskEmpty); + } - //Uncomment next line to revert to old exclusion mask file usage. Remove once it is passed. -#define OLDWAY + /* + * Apply exclude mask from file + */ + loc_context.exclusionMaskSet = false; -#ifdef OLDWAY + char* exclusionMaskFileName = GetIonConfigFile(exclusionMaskFile.c_str()); -void SetExcludeMask (SpatialContext &loc_context, Mask *maskPtr, char *chipType, int rows, int cols) -{ + fprintf(stderr, "Exclusion Mask File = '%s'\n", exclusionMaskFileName); - bool applyExclusionMask = true; - - /* - * Determine if this is a cropped dataset - * 3 types: - * wholechip image dataset - rows,cols should be == to chip_len_x,chip_len_y - * cropped image dataset - above test is false AND chip_offset_x == -1 - * blocked image dataset - above test is false AND chip_offset_x != -1 - */ - if ( (rows == loc_context.chip_len_y) && (cols == loc_context.chip_len_x)) - { - //This is a wholechip dataset - applyExclusionMask = true; - fprintf (stderr, "This is a wholechip dataset so the exclusion mask will be applied Chip %s\n",chipType); - } - else - { - if (loc_context.chip_offset_x == -1) - { - applyExclusionMask = false; - fprintf (stderr, "This is a cropped dataset so the exclusion mask will not be applied\n"); - fprintf (stderr, "rows=%d, chip_rows=%d,cols=%d, chip_cols=%d for chip %s\n",rows, loc_context.chip_len_y,cols, loc_context.chip_len_x, chipType); - } - else - { - applyExclusionMask = false; - fprintf (stderr, "This is a block dataset so the exclusion mask will not be applied\n"); + if (exclusionMaskFileName) { + loc_context.exclusionMaskSet = true; - } - } - /* - * If we get a cropped region definition from the command line, we want the whole chip to be MaskExclude - * except for the defined crop region(s) which are marked MaskEmpty. If no cropRegion defined on command line, - * then we proceed with marking the entire chip MaskEmpty - */ - if (loc_context.numCropRegions == 0) - { - maskPtr->Init (cols, rows, MaskEmpty); - } - else - { - maskPtr->Init (cols, rows, MaskExclude); - - // apply one or more cropRegions, mark them MaskEmpty - for (int q = 0; q < loc_context.numCropRegions; q++) - { - maskPtr->MarkRegion (loc_context.cropRegions[q], MaskEmpty); - } - } - - /* - * Apply exclude mask from file - */ - loc_context.exclusionMaskSet = false; - if (chipType && applyExclusionMask) - { - char *exclusionMaskFileName = NULL; - char filename[64] = { 0 }; - - sprintf (filename, "exclusionMask_%s.bin", chipType); - - exclusionMaskFileName = GetIonConfigFile (filename); - fprintf (stderr, "Exclusion Mask File = '%s'\n", exclusionMaskFileName); - if (exclusionMaskFileName) - { - loc_context.exclusionMaskSet = true; - - Mask excludeMask (1, 1); - excludeMask.SetMask (exclusionMaskFileName); - free (exclusionMaskFileName); - //--- Mark beadfind masks with MaskExclude bits from exclusionMaskFile - maskPtr->SetThese (&excludeMask, MaskExclude); + Mask excludeMask(loc_context.cols, loc_context.rows); - } - else - { - fprintf (stderr, "WARNING: Exclusion Mask %s not applied\n", filename); - } - } -} + if (exclusionMaskFile.compare(exclusionMaskFile.size() - 3, 3, "txt") == 0) { // this is text mask (e.g. for proton/S5) + std::cerr << "Using text exclusion mask\n"; -#else + // SpatialContext sets offsets to -1 if it can't parse them from the working directory + // We, in principle, want to enable handling of a PGM .txt mask + excludeMask.SetMaskFullChipText( exclusionMaskFileName, + max(0,loc_context.chip_offset_x), max(0,loc_context.chip_offset_y), + loc_context.cols, loc_context.rows ); -void SetExcludeMask (SpatialContext &loc_context, Mask *maskPtr, char *chipType, int rows, int cols) -{ + //--- Mark beadfind masks with MaskExclude bits from exclusionMaskFile + maskPtr->SetThese(&excludeMask, MaskExclude); + } else { // this is binary mask + std::cerr << "Using binary exclusion mask\n"; + excludeMask.SetMask(exclusionMaskFileName); - bool applyExclusionMask = true; - - /* - * Determine if this is a cropped dataset - * 3 types: - * wholechip image dataset - rows,cols should be == to chip_len_x,chip_len_y - * cropped image dataset - above test is false AND chip_offset_x == -1 - * blocked image dataset - above test is false AND chip_offset_x != -1 - */ - if ( (rows == loc_context.chip_len_y) && (cols == loc_context.chip_len_x)) - { - //This is a wholechip dataset - applyExclusionMask = true; - fprintf (stderr, "This is a wholechip dataset so the exclusion mask will be applied\n"); - } - else - { - if (loc_context.chip_offset_x == -1) - { - applyExclusionMask = false; - fprintf (stderr, "This is a cropped dataset so the exclusion mask will not be applied\n"); - } - else - { - applyExclusionMask = true; - fprintf (stderr, "This is a block dataset so the exclusion mask will be applied\n"); - } - } - /* - * If we get a cropped region definition from the command line, we want the whole chip to be MaskExclude - * except for the defined crop region(s) which are marked MaskEmpty. If no cropRegion defined on command line, - * then we proceed with marking the entire chip MaskEmpty - */ - if (loc_context.numCropRegions == 0) - { - maskPtr->Init (cols, rows, MaskEmpty); - } - else - { - maskPtr->Init (cols, rows, MaskExclude); - - // apply one or more cropRegions, mark them MaskEmpty - for (int q = 0; q < loc_context.numCropRegions; q++) - { - maskPtr->MarkRegion (loc_context.cropRegions[q], MaskEmpty); - } - } - - /* - * Apply exclude mask from file - */ - loc_context.exclusionMaskSet = false; - if (chipType && applyExclusionMask) - { - char *exclusionMaskFileName = NULL; - char filename[64] = { 0 }; - - sprintf (filename, "excludeMask_%s", chipType); - - exclusionMaskFileName = GetIonConfigFile (filename); - fprintf (stderr, "Exclusion Mask File = '%s'\n", exclusionMaskFileName); - if (exclusionMaskFileName) - { - loc_context.exclusionMaskSet = true; - - FILE *excludeFile = NULL; - excludeFile = fopen (exclusionMaskFileName,"rb"); - assert (excludeFile != NULL); - uint16_t x = 0; - uint16_t y = 0; - while (1) - { - if (fread (&x, sizeof (x), 1, excludeFile) != 1) break; - if (fread (&y, sizeof (y), 1, excludeFile) != 1) break; - //fprintf (stderr, "Excluding %d %d (%d %d)\n",x,y,(int) x - chip_offset_x,(int) y - chip_offset_y); - maskPtr->Set ( (int) x - loc_context.chip_offset_x, (int) y - loc_context.chip_offset_y,MaskExclude); - } + //--- Mark beadfind masks with MaskExclude bits from exclusionMaskFile + maskPtr->SetThese(&excludeMask, MaskExclude); + } + } else if (chipType) { + std::cerr << "Using binary exclusion mask based on chip type: " + << chipType << "\n"; + char filename[64] = { 0 }; + sprintf(filename, "exclusionMask_%s.bin", chipType); + + exclusionMaskFileName = GetIonConfigFile(filename); + fprintf(stderr, "Exclusion Mask File = '%s'\n", exclusionMaskFileName); + if (exclusionMaskFileName) { + loc_context.exclusionMaskSet = true; + + Mask excludeMask(1, 1); + excludeMask.SetMask(exclusionMaskFileName); + + //--- Mark beadfind masks with MaskExclude bits from exclusionMaskFile + maskPtr->SetThese(&excludeMask, MaskExclude); + + } else { + fprintf(stderr, "WARNING: Exclusion Mask %s not applied\n", filename); + } + } + free(exclusionMaskFileName); + } else { + std::cerr << "Exclusion mask is not applied"; } - else - { - fprintf (stderr, "WARNING: Exclusion Mask %s not applied\n", filename); - } - } } - -#endif diff --git a/Analysis/AnalysisOrg/MaskFunctions.h b/Analysis/AnalysisOrg/MaskFunctions.h index 96de6f71..da50f2c1 100644 --- a/Analysis/AnalysisOrg/MaskFunctions.h +++ b/Analysis/AnalysisOrg/MaskFunctions.h @@ -5,7 +5,8 @@ #include "Mask.h" #include "CommandLineOpts.h" -void SetExcludeMask(SpatialContext &loc_context, Mask *maskPtr, char *chipType, int rows, int cols); +void SetExcludeMask(SpatialContext &loc_context, Mask *maskPtr, char *chipType, int rows, int cols, + std::string exclusionMaskFileName, bool beadfindThumbnail); #endif // MASKFUNCTIONS_H diff --git a/Analysis/AnalysisOrg/ProcessImageToWell.cpp b/Analysis/AnalysisOrg/ProcessImageToWell.cpp index 1dc9e40f..49f34b06 100644 --- a/Analysis/AnalysisOrg/ProcessImageToWell.cpp +++ b/Analysis/AnalysisOrg/ProcessImageToWell.cpp @@ -529,8 +529,8 @@ void ImageToWells::MoveWellsFileAndFinalize() inception_state.loc_context.chip_offset_y, inception_state.loc_context.chip_offset_y + inception_state.loc_context.rows - 1, inception_state.loc_context.chip_len_y) ){ - printf("Masking DataCollect exclude regions as Bad Key\n"); - FromBeadfindMask.my_mask->SetAll((MaskType)(MaskFilteredBadKey)); // MaskIgnore + printf("Masking DataCollect exclude regions as Ignore\n"); + FromBeadfindMask.my_mask->SetAll((MaskType)(MaskIgnore)); } } diff --git a/Analysis/AnalysisOrg/SpatialContext.cpp b/Analysis/AnalysisOrg/SpatialContext.cpp index 98da13ff..281550ce 100644 --- a/Analysis/AnalysisOrg/SpatialContext.cpp +++ b/Analysis/AnalysisOrg/SpatialContext.cpp @@ -20,6 +20,7 @@ void SpatialContext::DefaultSpatialContext() regionYSize = 0; cropRegions = NULL; numCropRegions = 0; + isCropped = false; // some raw image processing (like cross talk correction in the Image class) needs the absolute coordinates of the // pixels in the image. This is easy for a standard data set, but for a cropped data set the origin of the data is // unknown. These allow the user to specify the location of the cropped region so that these parts of analysis @@ -137,6 +138,7 @@ void SpatialContext::SetOpts(OptArgs &opts, Json::Value& json_params) cropRegions[numCropRegions-1].row = vec2[1]; cropRegions[numCropRegions-1].w = vec2[2]; cropRegions[numCropRegions-1].h = vec2[3]; + isCropped = true; } else { diff --git a/Analysis/AnalysisOrg/SpatialContext.h b/Analysis/AnalysisOrg/SpatialContext.h index 648df368..fc8b42a3 100644 --- a/Analysis/AnalysisOrg/SpatialContext.h +++ b/Analysis/AnalysisOrg/SpatialContext.h @@ -25,6 +25,7 @@ class SpatialContext{ int regionsY; Region *cropRegions; int numCropRegions; + bool isCropped; int cropped_region_x_offset; int cropped_region_y_offset; int chip_offset_x; diff --git a/Analysis/AnalysisOrg/cudaWrapper.cpp b/Analysis/AnalysisOrg/cudaWrapper.cpp index 8435dbeb..6d86629d 100644 --- a/Analysis/AnalysisOrg/cudaWrapper.cpp +++ b/Analysis/AnalysisOrg/cudaWrapper.cpp @@ -124,8 +124,9 @@ void gpuDeviceConfig::initDeviceContexts(){ it++; } catch(cudaException &e) { + //throw cudaExecutionException(e.getCudaError(),__FILE__,__LINE__); cout << e.what() << endl; - cout << "CUDA "<< *it << ": Context could not be created. Can't use this GPU." << *it << endl; + cout << "CUDA "<< *it << ": gpuDeviceConfig::initDeviceContexts: Context could not be created. removing device with id: "<< *it << " from valid device list" << endl; it = validDevices.erase (it); } } @@ -196,7 +197,7 @@ bool gpuDeviceConfig::setValidDevices(std::vector &CmdlineDeviceList, bool initDeviceContexts(); - if(! validDevices.size() > 0 ) + if(! (validDevices.size() > 0) ) cout << "CUDA: gpuDeviceConfig: no context could be created, defaulting to CPU only execution" << endl; else cudaSetValidDevices( &validDevices[0], int( validDevices.size())); diff --git a/Analysis/AnalysisOrg/justBeadFind/SeparatorInterface.cpp b/Analysis/AnalysisOrg/justBeadFind/SeparatorInterface.cpp index 79dacece..dbd2b4fd 100644 --- a/Analysis/AnalysisOrg/justBeadFind/SeparatorInterface.cpp +++ b/Analysis/AnalysisOrg/justBeadFind/SeparatorInterface.cpp @@ -78,6 +78,8 @@ void DoDiffSeparatorFromCLO (DifferentialSeparator *diffSeparator, CommandLineOp opts.useMeshNeighbors = 0; opts.regionXSize = inception_state.loc_context.regionXSize; opts.regionYSize = inception_state.loc_context.regionYSize; + opts.beadfindAcqThreshold = inception_state.bfd_control.beadfindAcqThreshold; + opts.beadfindBfThreshold = inception_state.bfd_control.beadfindBfThreshold; // For saving dcOffset and NucStep opts.nucStepDir = string ( opts.analysisDir + string ( "/NucStepFromBeadfind" )); @@ -209,7 +211,7 @@ void IsolatedBeadFind ( char *results_folder, string &analysisLocation, SeqListClass &my_keys, TrackProgress &my_progress, - string& chipTpye) + string& chipType) { /********************************************************************* // Beadfind Section @@ -217,7 +219,7 @@ void IsolatedBeadFind ( Mask bfmask ( 1, 1 ); Mask *maskPtr = &bfmask; // beadfind has responsibility for defining the exclusion mask - SetExcludeMask ( inception_state.loc_context,maskPtr,(char*)chipTpye.c_str(),my_image_spec.rows,my_image_spec.cols ); + SetExcludeMask ( inception_state.loc_context,maskPtr,(char*)chipType.c_str(),my_image_spec.rows,my_image_spec.cols, inception_state.bfd_control.exclusionMaskFile, inception_state.bfd_control.beadfindThumbnail ); bool beadfind_done = false; if ( inception_state.mod_control.reusePriorBeadfind ) { diff --git a/Analysis/BaseCaller/BarcodeClassifier.cpp b/Analysis/BaseCaller/BarcodeClassifier.cpp index 5420292f..7c39ec2f 100644 --- a/Analysis/BaseCaller/BarcodeClassifier.cpp +++ b/Analysis/BaseCaller/BarcodeClassifier.cpp @@ -55,9 +55,10 @@ BarcodeClassifier::BarcodeClassifier(OptArgs& opts, BarcodeDatasets& datasets, c hamming_dmin_ = -1; barcode_min_start_flow_ = -1; no_barcode_read_group_ = -1; + have_ambiguity_codes_ = false; - dataset_in_use_ = datasets.DatasetInUse(); - is_control_dataset_ = datasets.IsControlDataset(); + dataset_in_use_ = datasets.DatasetInUse(); + is_control_dataset_ = datasets.IsControlDataset(); // --- Prepare directory structure and output files @@ -122,7 +123,7 @@ BarcodeClassifier::BarcodeClassifier(OptArgs& opts, BarcodeDatasets& datasets, c LoadBarcodesFromDataset(datasets, keys); // For now only option to get the properties of the barcode set - if (compute_dmin or score_auto_config_) + if ((compute_dmin or score_auto_config_) and not have_ambiguity_codes_) ComputeHammingDistance(); // Set options with limits that potentially depend on barcode set Hamming distance @@ -193,6 +194,12 @@ void BarcodeClassifier::LoadBarcodesFromDataset(BarcodeDatasets& datasets, const barcode_.back().full_barcode += read_group.get("barcode_adapter","").asString(); int key_barcode_adapter_length = barcode_.back().full_barcode.length(); + // Check for non-ACGT characters + if (std::string::npos != barcode_.back().full_barcode.find_first_not_of("ACGTN")){ + have_ambiguity_codes_ = true; + continue; + } + int flow = 0; int curBase = 0; int end_length = -1; @@ -239,17 +246,23 @@ void BarcodeClassifier::LoadBarcodesFromDataset(BarcodeDatasets& datasets, const if (dataset_in_use_ and no_barcode_read_group_ < 0) cout << " INFO: Dataset " << barcode_id << " does not have a non-barcoded read group." << endl; - // And loop through barcodes again to determine maximum amount of flows after start flow has been determined - int barcode_flows, barcode_min_flows = -1; - for (unsigned int bc=0; bc= 0 and barcode_min_flows != barcode_max_flows_) + cout << " WARNING: Barcode set is not flow space synchronized. Barcodes range from " + << barcode_min_flows << " to " << barcode_max_flows_ << " flows." << endl; } - if (dataset_in_use_ and barcode_min_flows >= 0 and barcode_min_flows != barcode_max_flows_) - cout << " WARNING: Barcode set is not flow space synchronized. Barcodes range from " - << barcode_min_flows << " to " << barcode_max_flows_ << " flows." << endl; + /*cout << " Barcode dataset info: min_start_flow=" << barcode_min_start_flow_ << endl << + " barcode_max_flows=" << barcode_max_flows_ << endl << + " num_flows=" << barcode_.back().num_flows << endl; + */ // Export barcode_max_flows_ to datasets structure datasets.SetBCmaxFlows(barcode_max_flows_); @@ -264,7 +277,12 @@ void BarcodeClassifier::SetClassificationParams(int mode, double cutoff, double score_cutoff_ = cutoff; score_separation_ = separation; - CheckParameterLowerUpperBound("barcode-mode", score_mode_, 1,1, 5,1, 2); + CheckParameterLowerUpperBound("barcode-mode", score_mode_, 0,1, 5,1, 2); + if (have_ambiguity_codes_ and score_mode_ != 0){ + cout << " WARNING: Non-ACGT characters detected. Setting score-mode=0." << endl; + score_mode_ =0; + return; + } // Do we have minimum distance information available? if (hamming_dmin_ > 0) { @@ -365,9 +383,29 @@ void BarcodeClassifier::BuildPredictedSignals(float cf, float ie, float dr) } +// ------------------------------------------------------------------------ +// trivially simple base space matching + +int BarcodeClassifier::SimpleBaseSpaceClassification(const BasecallerRead& basecaller_read) +{ + int bc_len; + for (int bc = 0; bc < num_barcodes_; ++bc) { + bc_len = barcode_[bc].full_barcode.length(); + if (basecaller_read.sequence.size() < barcode_[bc].full_barcode.length()) + continue; + + int i=0; + while (i& base_to_flow, int& best_errors) +int BarcodeClassifier::FlowAlignClassification(const ProcessedRead &processed_read, const vector& base_to_flow, int& best_errors) { int best_barcode = -1; best_errors = 1 + (int)score_cutoff_; // allows match with this many errors minimum when in bc_score_mode 1 @@ -599,25 +637,27 @@ void BarcodeClassifier::ClassifyAndTrimBarcode(int read_index, ProcessedRead &pr return; int best_barcode = -1; - // looks at flow-space absolute error counts, not ratios - if (score_mode_ == 1) { - best_barcode = BaseSpaceClassification(processed_read, base_to_flow, processed_read.barcode_n_errors); - } - // Minimize distance to barcode predicted signal - else if (score_mode_ == 2 or score_mode_ == 3) { - // Minimize L2 distance for score_mode_ == 2 - // Minimize L1 distance for score_mode_ == 3 - best_barcode = SignalSpaceClassification(basecaller_read, processed_read.barcode_distance, processed_read.barcode_n_errors, - processed_read.barcode_bias, processed_read.barcode_filt_zero_error); - } else if (score_mode_ ==4 or score_mode_ ==5){ - //L2 for score mode 4 - //L1 for score mode 5 + switch (score_mode_){ + case 1: // looks at flow-space absolute error counts, not ratios + best_barcode = FlowAlignClassification(processed_read, base_to_flow, processed_read.barcode_n_errors); + break; + case 2: // Minimize L2 distance for score_mode_ == 2 + case 3: // Minimize L1 distance for score_mode_ == 3 + best_barcode = SignalSpaceClassification(basecaller_read, processed_read.barcode_distance, processed_read.barcode_n_errors, + processed_read.barcode_bias, processed_read.barcode_filt_zero_error); + break; + case 4: //L2 for score mode 4 + case 5: //L1 for score mode 5 best_barcode = ProportionalSignalClassification(basecaller_read, processed_read.barcode_distance, processed_read.barcode_n_errors, - processed_read.barcode_bias, processed_read.barcode_filt_zero_error); + processed_read.barcode_bias, processed_read.barcode_filt_zero_error); + break; + default: // Trivial base space comparison + best_barcode = SimpleBaseSpaceClassification(basecaller_read); } // Optionally verify barcode adapter - AdapterValidation(basecaller_read, best_barcode, processed_read.barcode_adapter_filtered); + if (score_mode_ > 0) + AdapterValidation(basecaller_read, best_barcode, processed_read.barcode_adapter_filtered); // -------- Classification done, now accounting ---------- @@ -645,14 +685,19 @@ void BarcodeClassifier::ClassifyAndTrimBarcode(int read_index, ProcessedRead &pr return; // Account for barcode + barcode adapter bases - processed_read.filter.n_bases_barcode = 0; - while (processed_read.filter.n_bases_barcode < processed_read.filter.n_bases and base_to_flow[processed_read.filter.n_bases_barcode] < bce.num_flows-1) - processed_read.filter.n_bases_barcode++; - - int last_homopolymer = bce.last_homopolymer; - while (processed_read.filter.n_bases_barcode < processed_read.filter.n_bases and base_to_flow[processed_read.filter.n_bases_barcode] < bce.num_flows and last_homopolymer > 0) { - processed_read.filter.n_bases_barcode++; - last_homopolymer--; + if (score_mode_ == 0){ + processed_read.filter.n_bases_barcode = min(processed_read.filter.n_bases, (int)barcode_[best_barcode].full_barcode.length()); + } + else { + processed_read.filter.n_bases_barcode = 0; + while (processed_read.filter.n_bases_barcode < processed_read.filter.n_bases and base_to_flow[processed_read.filter.n_bases_barcode] < bce.num_flows-1) + processed_read.filter.n_bases_barcode++; + + int last_homopolymer = bce.last_homopolymer; + while (processed_read.filter.n_bases_barcode < processed_read.filter.n_bases and base_to_flow[processed_read.filter.n_bases_barcode] < bce.num_flows and last_homopolymer > 0) { + processed_read.filter.n_bases_barcode++; + last_homopolymer--; + } } // Propagate current 5' trimming point diff --git a/Analysis/BaseCaller/BarcodeClassifier.h b/Analysis/BaseCaller/BarcodeClassifier.h index 48a0c31c..bbc7d944 100644 --- a/Analysis/BaseCaller/BarcodeClassifier.h +++ b/Analysis/BaseCaller/BarcodeClassifier.h @@ -55,7 +55,9 @@ class BarcodeClassifier { void SetClassificationParams(int mode, double cutoff, double separation); - int BaseSpaceClassification(const ProcessedRead &processed_read, const vector& base_to_flow, int& best_errors); + int SimpleBaseSpaceClassification(const BasecallerRead& basecaller_read); + + int FlowAlignClassification(const ProcessedRead &processed_read, const vector& base_to_flow, int& best_errors); int SignalSpaceClassification(const BasecallerRead& basecaller_read, float& best_distance, int& best_errors, vector& best_bias, int& filtered_zero_errors); @@ -147,6 +149,7 @@ class BarcodeClassifier { bool barcode_filter_named_; // Exclude barcodes with an associated sample name from filtering bool barcode_ignore_flows_; // Switch telling the classifier to exclude certain flows from classification bool trim_barcodes_; // Switch to trim or leave barcodes alone + bool have_ambiguity_codes_; // Do we have non-ACGT characters in key, barcode? vector classifier_ignore_flows_; // Specifying an interval of flows to exclude from classification int hamming_dmin_; diff --git a/Analysis/BaseCaller/BaseCaller.cpp b/Analysis/BaseCaller/BaseCaller.cpp index 6bfc1611..068eaae1 100644 --- a/Analysis/BaseCaller/BaseCaller.cpp +++ b/Analysis/BaseCaller/BaseCaller.cpp @@ -217,9 +217,10 @@ void ClassifyAndSampleWells(BaseCallerContext & bc, const BCwellSampling & Sampl void scaleup_flowgram(const vector& sigIn, vector& sigOut, int max_flow) { - if (sigOut.size() != (size_t)max_flow) - sigOut.resize(max_flow); - for (int flow = 0; flow < max_flow; ++flow){ + int safe_max = min(max_flow, (int)sigIn.size()); + if (sigOut.size() != (size_t)safe_max) + sigOut.resize(safe_max); + for (int flow = 0; flow < safe_max; ++flow){ int v = 128*sigIn[flow]; // handle overflow if (v < -16383 or v > 16383) { @@ -230,6 +231,19 @@ void scaleup_flowgram(const vector& sigIn, vector& sigOut, int m } +void make_base_to_flow(const vector& sequence,ion::FlowOrder& flow_order, vector& base_to_flow,vector& flow_to_base,int num_flows) +{ + int nBases = sequence.size(); + base_to_flow.resize(nBases); + flow_to_base.assign(num_flows,-1); + + for (int base = 0, flow = 0; base < nBases; ++base) { + while (flow < num_flows and sequence.at(base) != flow_order[flow]) + flow++; + base_to_flow.at(base) = flow; + flow_to_base.at(flow) = base; + } +} // -------------------------------------------------------------------------- //! @brief Main function for BaseCaller executable Mark: XXX @@ -389,7 +403,7 @@ int main (int argc, const char *argv[]) ClassifyAndSampleWells(bc, bc_params.GetSamplingOpts()); // Find distribution of clonal reads for use in read filtering: - filters.TrainClonalFilter(bc_params.GetFiles().output_directory, wells, mask, bc.polyclonal_filter); + filters.TrainClonalFilter(bc_params.GetFiles().output_directory, wells, mask); MemUsage("ClonalPopulation"); ReportState(analysis_start_time,"Polyclonal Filter Training Complete"); @@ -425,29 +439,31 @@ int main (int argc, const char *argv[]) // Library data set writer - always bc.lib_writer.Open(bc_params.GetFiles().output_directory, datasets, 0, bc.chip_subset.NumRegions(), bc.flow_order, bc.keys[0].bases(), filters.GetLibBeadAdapters(), bc_params.NumBamWriterThreads(), - basecaller_json, bam_comments, tag_trimmer, barcodes.TrimBarcodes()); + basecaller_json, bam_comments, tag_trimmer, barcodes.TrimBarcodes(), bc_params.CompressOutputBam()); // Calibration reads data set writer - if applicable if (bc.have_calibration_panel) bc.calib_writer.Open(bc_params.GetFiles().output_directory, datasets_calibration, 0, bc.chip_subset.NumRegions(), bc.flow_order, bc.keys[0].bases(), filters.GetLibBeadAdapters(), bc_params.NumBamWriterThreads(), - basecaller_json, bam_comments, tag_trimmer, barcodes.TrimBarcodes()); + basecaller_json, bam_comments, tag_trimmer, barcodes.TrimBarcodes(), bc_params.CompressOutputBam()); // Test fragments data set writer - if applicable if (bc.process_tfs) bc.tf_writer.Open(bc_params.GetFiles().output_directory, datasets_tf, 1, bc.chip_subset.NumRegions(), bc.flow_order, bc.keys[1].bases(), filters.GetTFBeadAdapters(), bc_params.NumBamWriterThreads(), - basecaller_json, bam_comments, tag_trimmer, barcodes.TrimBarcodes()); + basecaller_json, bam_comments, tag_trimmer, barcodes.TrimBarcodes(), bc_params.CompressOutputBam()); // Unfiltered / unfiltered untrimmed data set writers - if applicable if (!bc.unfiltered_set.empty()) { bc.unfiltered_writer.Open(bc_params.GetFiles().unfiltered_untrimmed_directory, datasets_unfiltered_untrimmed, -1, bc.chip_subset.NumRegions(), bc.flow_order, bc.keys[0].bases(), filters.GetLibBeadAdapters(), - bc_params.NumBamWriterThreads(), basecaller_json, bam_comments, tag_trimmer, barcodes.TrimBarcodes()); + bc_params.NumBamWriterThreads(), basecaller_json, bam_comments, tag_trimmer, barcodes.TrimBarcodes(), + bc_params.CompressOutputBam()); bc.unfiltered_trimmed_writer.Open(bc_params.GetFiles().unfiltered_trimmed_directory, datasets_unfiltered_trimmed, -1, bc.chip_subset.NumRegions(), bc.flow_order, bc.keys[0].bases(), filters.GetLibBeadAdapters(), - bc_params.NumBamWriterThreads(), basecaller_json, bam_comments, tag_trimmer, barcodes.TrimBarcodes()); + bc_params.NumBamWriterThreads(), basecaller_json, bam_comments, tag_trimmer, barcodes.TrimBarcodes(), + bc_params.CompressOutputBam()); } // @@ -576,6 +592,7 @@ void * BasecallerWorker(void *input) vector filtering_details(13,0); vector quality(3*num_flows); vector base_to_flow (3*num_flows); //!< Flow of in-phase incorporation of each base. + vector flow_to_base (num_flows,-1); //!< base pos of each flow, -1 if not available DPTreephaser treephaser(bc.flow_order, bc.windowSize); treephaser.SetStateProgression(bc.diagonal_state_prog); @@ -731,7 +748,7 @@ void * BasecallerWorker(void *input) } // Get rid of outliers quickly - bc.filters->FilterHighPPFAndPolyclonal (read_index, read_class, processed_read.filter, read.raw_measurements, bc.polyclonal_filter); + bc.filters->FilterHighPPFAndPolyclonal (read_index, read_class, processed_read.filter, read.raw_measurements); if (not key_pass) bc.filters->FilterFailedKeypass (read_index, read_class, processed_read.filter, read.sequence); if (!is_random_unfiltered and !bc.filters->IsValid(read_index)) // No reason to waste more time @@ -788,7 +805,10 @@ void * BasecallerWorker(void *input) } // Compute QV metrics on pot. calibrated sequence - treephaser_sse.ComputeQVmetrics(read); + // Generate base_to_flow before ComputeQVmetrics. But not too early, otherwise the sequence is not ready + make_base_to_flow(read.sequence,bc.flow_order,base_to_flow,flow_to_base,num_flows); + + treephaser_sse.ComputeQVmetrics_flow(read,flow_to_base,bc.flow_predictors_); compute_base_calls = false; } #endif @@ -817,7 +837,11 @@ void * BasecallerWorker(void *input) bc.histogram_calibration->PolishRead(bc.flow_order, x+bc.chip_subset.GetColOffset(), y+bc.chip_subset.GetRowOffset(), read, bc.linear_cal_model); } - treephaser.ComputeQVmetrics(read); + // Compute QV metrics on pot. calibrated sequence + // Generate base_to_flow before ComputeQVmetrics. But not too early, otherwise the sequence is not ready + make_base_to_flow(read.sequence,bc.flow_order,base_to_flow,flow_to_base,num_flows); + + treephaser.ComputeQVmetrics_flow(read,flow_to_base,bc.flow_predictors_); } // Misc data management: Generate residual, scaled_residual @@ -829,17 +853,6 @@ void * BasecallerWorker(void *input) // Misc data management: Put base calls in proper string form processed_read.filter.CalledRead(read.sequence.size()); - // Misc data management: Generate base_to_flow - - base_to_flow.clear(); - base_to_flow.reserve(processed_read.filter.n_bases); - for (int base = 0, flow = 0; base < processed_read.filter.n_bases; ++base) { - while (flow < num_flows and read.sequence[base] != bc.flow_order[flow]) - flow++; - base_to_flow.push_back(flow); - } - - // Misc data management: Populate some trivial read properties char read_name[256]; @@ -862,23 +875,58 @@ void * BasecallerWorker(void *input) // Predictor 5 - Treephaser: Penalty indicating deletion after the called base // Predictor 6 - Neighborhood noise - mean of 'noise' +-5 BASES around a base. Noise is mean{abs(val - round(val))} - int num_predictor_bases = min(num_flows, processed_read.filter.n_bases); - - PerBaseQual::PredictorLocalNoise(local_noise, num_predictor_bases, base_to_flow, read.normalized_measurements, read.prediction); - PerBaseQual::PredictorNeighborhoodNoise(neighborhood_noise, num_predictor_bases, base_to_flow, read.normalized_measurements, read.prediction); - //PerBaseQual::PredictorNoiseOverlap(minus_noise_overlap, num_predictor_bases, read.normalized_measurements, read.prediction); - PerBaseQual::PredictorBeverlyEvents(minus_noise_overlap, num_predictor_bases, base_to_flow, scaled_residual); - PerBaseQual::PredictorHomopolymerRank(homopolymer_rank, num_predictor_bases, read.sequence); + //char short_name[20]; // x:y only + //sprintf(short_name, "%05d:%05d", bc.chip_subset.GetRowOffset() + y, bc.chip_subset.GetColOffset() + x); + //bc.quality_generator.GenerateBaseQualities(short_name, processed_read.filter.n_bases, num_flows, + vector homopolymer_rank_flow(num_flows, 0); + bool use_flow_predictors = bc.flow_predictors_; + if (bc.quality_generator.toSavePredictors()) { + int num_predictor_bases = min(num_flows, processed_read.filter.n_bases); + PerBaseQual::PredictorLocalNoise(local_noise, num_predictor_bases, base_to_flow, read.normalized_measurements, read.prediction,use_flow_predictors); + PerBaseQual::PredictorNeighborhoodNoise(neighborhood_noise, num_predictor_bases, base_to_flow, read.normalized_measurements, read.prediction,use_flow_predictors); + //PerBaseQual::PredictorNoiseOverlap(minus_noise_overlap, num_predictor_bases, read.normalized_measurements, read.prediction,use_flow_predictors); + PerBaseQual::PredictorBeverlyEvents(minus_noise_overlap, num_predictor_bases, base_to_flow, scaled_residual,use_flow_predictors); + PerBaseQual::PredictorHomopolymerRank(homopolymer_rank, num_predictor_bases, read.sequence, homopolymer_rank_flow, flow_to_base, use_flow_predictors); + + bc.quality_generator.DumpPredictors(read_name, processed_read.filter.n_bases, num_flows, + read.penalty_residual, local_noise, minus_noise_overlap, // <- predictors 1,2,3 + homopolymer_rank, read.penalty_mismatch, neighborhood_noise, // <- predictors 4,5,6 + base_to_flow, quality, + read.additive_correction, + read.multiplicative_correction, + read.state_inphase, + read.penalty_residual_flow, + read.penalty_mismatch_flow, + homopolymer_rank_flow, + flow_to_base, + use_flow_predictors); + } - quality.clear(); - bc.quality_generator.GenerateBaseQualities(processed_read.bam.Name, processed_read.filter.n_bases, num_flows, + // 8/18/2016, ew: still use base-predictors to predict quality scores for now + use_flow_predictors = false; + bool redo_genPred = ((! bc.quality_generator.toSavePredictors()) || bc.flow_predictors_) ? true:false; + //redo_genPred = true; + if (redo_genPred) { + int num_predictor_bases = min(num_flows, processed_read.filter.n_bases); + PerBaseQual::PredictorLocalNoise(local_noise, num_predictor_bases, base_to_flow, read.normalized_measurements, read.prediction,use_flow_predictors); + PerBaseQual::PredictorNeighborhoodNoise(neighborhood_noise, num_predictor_bases, base_to_flow, read.normalized_measurements, read.prediction,use_flow_predictors); + //PerBaseQual::PredictorNoiseOverlap(minus_noise_overlap, num_predictor_bases, read.normalized_measurements, read.prediction,use_flow_predictors); + PerBaseQual::PredictorBeverlyEvents(minus_noise_overlap, num_predictor_bases, base_to_flow, scaled_residual,use_flow_predictors); + PerBaseQual::PredictorHomopolymerRank(homopolymer_rank, num_predictor_bases, read.sequence, homopolymer_rank_flow, flow_to_base, use_flow_predictors); + } + //bc.quality_generator.GenerateBaseQualities(processed_read.bam.Name, processed_read.filter.n_bases, num_flows, + bc.quality_generator.GenerateBaseQualities(read_name, processed_read.filter.n_bases, num_flows, read.penalty_residual, local_noise, minus_noise_overlap, // <- predictors 1,2,3 homopolymer_rank, read.penalty_mismatch, neighborhood_noise, // <- predictors 4,5,6 base_to_flow, quality, read.additive_correction, read.multiplicative_correction, - read.state_inphase); - + read.state_inphase, + read.penalty_residual_flow, + read.penalty_mismatch_flow, + homopolymer_rank_flow, + flow_to_base, + use_flow_predictors); // // Step 4a. Barcode classification of library reads @@ -947,9 +995,13 @@ void * BasecallerWorker(void *input) // Step 4b. Add flow signal information to ZM tag in BAM record. // - int max_flow = min(num_flows,16); - if (processed_read.filter.n_bases_filtered > 0) + int max_flow = num_flows; + if (bc.trim_zm){ + if (processed_read.filter.n_bases_filtered > 0) max_flow = min(num_flows, base_to_flow[processed_read.filter.n_bases_filtered-1] + 16); + else + max_flow = min(num_flows,16); + } scaleup_flowgram(read.normalized_measurements,flowgram2,max_flow); processed_read.bam.AddTag("ZM", flowgram2); @@ -960,8 +1012,10 @@ void * BasecallerWorker(void *input) processed_read.bam.AddTag("Yb", flowgram2); scaleup_flowgram(read.raw_measurements,flowgram2,max_flow); processed_read.bam.AddTag("Yw", flowgram2); - scaleup_flowgram(read.not_calibrated_measurements,flowgram2,max_flow); - processed_read.bam.AddTag("Yx", flowgram2); + if (bc.histogram_calibration->is_enabled()) { + scaleup_flowgram(read.not_calibrated_measurements,flowgram2,max_flow); + processed_read.bam.AddTag("Yx", flowgram2); + } } // diff --git a/Analysis/BaseCaller/BaseCallerFilters.cpp b/Analysis/BaseCaller/BaseCallerFilters.cpp index d772501f..1c178961 100644 --- a/Analysis/BaseCaller/BaseCallerFilters.cpp +++ b/Analysis/BaseCaller/BaseCallerFilters.cpp @@ -18,6 +18,7 @@ #include "IonErr.h" #include "RawWells.h" #include "Mask.h" +//#include "Utils.h" #include "BaseCallerUtils.h" #include "DPTreephaser.h" #include "OrderedDatasetWriter.h" @@ -490,7 +491,7 @@ void ReadFilteringStats::SaveToBasecallerJson(Json::Value &json, const string& c json["Filtering"]["ReadDetails"][class_name]["quality_trim"] = (Json::Int64)num_reads_removed_quality_trim_; json["Filtering"]["ReadDetails"][class_name]["quality_filter"] = (Json::Int64)num_reads_removed_quality_filt_; json["Filtering"]["ReadDetails"][class_name]["tag_trim"] = (Json::Int64)num_reads_removed_tag_trim_; - json["Filtering"]["ReadDetails"][class_name]["extra_trim"] = (Json::Int64)num_reads_removed_extra_trim_ + num_reads_removed_extra_trim_; + json["Filtering"]["ReadDetails"][class_name]["extra_trim"] = (Json::Int64)num_reads_removed_extra_trim_; json["Filtering"]["ReadDetails"][class_name]["valid"] = (Json::Int64)num_reads_final_; // BeadSummary - obsolete me! @@ -498,7 +499,7 @@ void ReadFilteringStats::SaveToBasecallerJson(Json::Value &json, const string& c json["BeadSummary"][class_name]["highPPF"] = (Json::Int64)(num_reads_removed_bkgmodel_high_ppf_ + num_reads_removed_high_ppf_); json["BeadSummary"][class_name]["zero"] = 0; json["BeadSummary"][class_name]["short"] = (Json::Int64)(num_reads_removed_short_ + num_reads_removed_adapter_trim_ - + num_reads_removed_quality_trim_ + num_reads_removed_tag_trim_); + + num_reads_removed_quality_trim_ + num_reads_removed_tag_trim_ +num_reads_removed_extra_trim_); json["BeadSummary"][class_name]["badKey"] = (Json::Int64)(num_reads_removed_bkgmodel_keypass_ + num_reads_removed_keypass_); json["BeadSummary"][class_name]["highRes"] = (Json::Int64)(num_reads_removed_residual_ + num_reads_removed_quality_filt_); json["BeadSummary"][class_name]["valid"] = (Json::Int64)num_reads_final_; @@ -530,7 +531,7 @@ void ReadFilteringStats::SaveToBasecallerJson(Json::Value &json, const string& c num_reads_removed_quality_trim_ + num_reads_removed_quality_filt_ + num_reads_removed_tag_trim_ + - num_bases_removed_extra_trim_); + num_reads_removed_extra_trim_); json["Filtering"]["LibraryReport"]["final_library_reads"] = (Json::Int64)num_reads_final_; // (3') Bead Adapters @@ -561,45 +562,43 @@ void ReadFilteringStats::SaveToBasecallerJson(Json::Value &json, const string& c void BaseCallerFilters::PrintHelp() { printf ("Read filtering and trimming options:\n"); - printf (" -d,--disable-all-filters on/off disable all filtering and trimming, overrides other args [off]\n"); + printf (" -d,--disable-all-filters on/off disable all filtering and trimming, overrides other args [off]\n"); printf (" Key-pass filter: \n"); - printf (" -k,--keypass-filter on/off apply keypass filter [on]\n"); - printf (" Polyclonal filter in BaseCaller:\n"); - printf (" --clonal-filter-solve on/off apply polyclonal filter [off]\n"); - printf (" --clonal-filter-tf on/off apply polyclonal filter to TFs [off]\n"); - printf (" --clonal-filter-maxreads INT maximum number of library reads used for polyclonal filter training [100000]\n"); - printf (" --min-read-length INT apply minimum read length filter [25]\n"); - printf (" --cr-filter on/off apply cafie residual filter [off]\n"); - printf (" --cr-filter-tf on/off apply cafie residual filter to TFs [off]\n"); - printf (" --cr-filter-max-value FLOAT cafie residual filter threshold [0.08]\n"); - printf (" --beverly-filter FLOAT,FLOAT filter_ratio,trim_ratio / off\n"); - printf (" apply Beverly filter/trimmer [off]\n"); - printf (" --qual-filter on/off apply quality filter based on expected number of errors [off]\n"); - printf (" --qual-filter-offset FLOAT error offset for expected errors quality filter [0.7]\n"); - printf (" --qual-filter-slope FLOAT expected errors allowed per base for expected errors quality filter [0.02]\n"); + printf (" -k,--keypass-filter on/off apply keypass filter [on]\n"); + printf (" Read filter settings:\n"); + printf (" --min-read-length INT apply minimum read length filter [25]\n"); + printf (" --cr-filter on/off apply cafie residual filter [off]\n"); + printf (" --cr-filter-tf on/off apply cafie residual filter to TFs [off]\n"); + printf (" --cr-filter-max-value FLOAT cafie residual filter threshold [0.08]\n"); + printf (" --beverly-filter FLOAT,FLOAT filter_ratio,trim_ratio / off\n"); + printf (" apply Beverly filter/trimmer [off]\n"); + printf (" --qual-filter on/off apply quality filter based on expected number of errors [off]\n"); + printf (" --qual-filter-offset FLOAT error offset for expected errors quality filter [0.7]\n"); + printf (" --qual-filter-slope FLOAT expected errors allowed per base for expected errors quality filter [0.02]\n"); printf ("\n"); + PolyclonalFilterOpts::PrintHelp(false); printf ("Read trimming options:\n"); - printf (" --trim-min-read-len INT reads trimmed shorter than this are omitted from output [min-read-length]\n"); - printf (" --trim-barcodes BOOL trim barcodes and barcode adapters [on]\n"); - printf (" --extra-trim-left INT Number of additional bases to remove from read start after prefix (key/barcode/tag) [0]\n"); - printf (" --extra-trim-right INT Number of additional bases to remove from read end before suffix (tag/adapter) [0]\n"); - printf (" --save-extra-trim BOOL Save extra trimmed bases to BAM tags (left ZE, right YE) [false]\n"); + printf (" --trim-min-read-len INT reads trimmed shorter than this are omitted from output [min-read-length]\n"); + printf (" --trim-barcodes BOOL trim barcodes and barcode adapters [on]\n"); + printf (" --extra-trim-left INT Number of additional bases to remove from read start after prefix (key/barcode/tag) [0]\n"); + printf (" --extra-trim-right INT Number of additional bases to remove from read end before suffix (tag/adapter) [0]\n"); + printf (" --save-extra-trim BOOL Save extra trimmed bases to BAM tags (left ZE, right YE) [false]\n"); printf (" Adapter trimming (turn off by supplying cutoff 0):\n"); - printf (" --trim-adapter-mode INT 0=use simplified metric, 1=use standard metric [1]\n"); - printf (" --trim-adapter STRING reverse complement of adapter sequence [ATCACCGACTGCCCATAGAGAGGCTGAGAC]\n"); - printf (" --trim-adapter-tf STRING/off adapter sequence for test fragments [off]\n"); - printf (" --trim-adapter-cutoff FLOAT cutoff for adapter trimming, 0=off [16]\n"); - printf (" --trim-adapter-min-match INT minimum adapter bases in the read required for trimming [6]\n"); + printf (" --trim-adapter-mode INT 0=use simplified metric, 1=use standard metric [1]\n"); + printf (" --trim-adapter STRING reverse complement of adapter sequence [ATCACCGACTGCCCATAGAGAGGCTGAGAC]\n"); + printf (" --trim-adapter-tf STRING/off adapter sequence for test fragments [off]\n"); + printf (" --trim-adapter-cutoff FLOAT cutoff for adapter trimming, 0=off [16]\n"); + printf (" --trim-adapter-min-match INT minimum adapter bases in the read required for trimming [6]\n"); printf (" Quality trimming (turn off by supplying mode 'off'):\n"); - printf (" --trim-qual-mode STRING select the method of quality trimming [\"sliding-window\"]\n"); - printf (" mode \"off\" : quality trimming disabled\n"); - printf (" mode \"sliding-window\" : sliding window quality trimming\n"); - printf (" mode \"expected-errors\" : quality trimming based on expected number of errors in read\n"); - printf (" mode \"all\" : shortest trimming point of all methods\n"); - printf (" --trim-qual-window-size INT window size for windowed quality trimming [30]\n"); - printf (" --trim-qual-cutoff FLOAT cutoff for windowed quality trimming, 100=off [15]\n"); - printf (" --trim-qual-offset FLOAT error threshold offset for expected error quality trimming [0.7]\n"); - printf (" --trim-qual-slope FLOAT increase of expected errors allowed for expected error quality trimming [0.005]\n"); + printf (" --trim-qual-mode STRING select the method of quality trimming [\"sliding-window\"]\n"); + printf (" mode \"off\" : quality trimming disabled\n"); + printf (" mode \"sliding-window\" : sliding window quality trimming\n"); + printf (" mode \"expected-errors\" : quality trimming based on expected number of errors in read\n"); + printf (" mode \"all\" : shortest trimming point of all methods\n"); + printf (" --trim-qual-window-size INT window size for windowed quality trimming [30]\n"); + printf (" --trim-qual-cutoff FLOAT cutoff for windowed quality trimming, 100=off [15]\n"); + printf (" --trim-qual-offset FLOAT error threshold offset for expected error quality trimming [0.7]\n"); + printf (" --trim-qual-slope FLOAT increase of expected errors allowed for expected error quality trimming [0.005]\n"); printf ("\n"); } @@ -613,6 +612,10 @@ BaseCallerFilters::BaseCallerFilters(OptArgs& opts, Json::Value &comments_json, num_classes_ = keys_.size(); assert(num_classes_ == 2); filter_mask_.assign(mask.H()*mask.W(), kUninitialized); + Json::Value null_json; + + cout << "Polyclonal filter settings:" << endl; + clonal_opts_.SetOpts(false, opts, null_json, flow_order_.num_flows()); // *** Retrieve filter command line options @@ -620,10 +623,6 @@ BaseCallerFilters::BaseCallerFilters(OptArgs& opts, Json::Value &comments_json, filter_min_read_length_ = max(opts.GetFirstInt ('-', "min-read-length", 25),1); trim_min_read_len_ = max(opts.GetFirstInt ('-', "trim-min-read-len", filter_min_read_length_),1); - filter_clonal_enabled_tfs_ = opts.GetFirstBoolean('-', "clonal-filter-tf", false); - filter_clonal_enabled_ = opts.GetFirstBoolean('-', "clonal-filter-solve", false); - filter_clonal_maxreads_ = opts.GetFirstInt ('-', "clonal-filter-maxreads", 100000); - filter_residual_enabled_ = opts.GetFirstBoolean('-', "cr-filter", false); filter_residual_enabled_tfs_ = opts.GetFirstBoolean('-', "cr-filter-tf", false); filter_residual_max_value_ = opts.GetFirstDouble ('-', "cr-filter-max-value", 0.08); @@ -633,7 +632,6 @@ BaseCallerFilters::BaseCallerFilters(OptArgs& opts, Json::Value &comments_json, filter_quality_slope_ = opts.GetFirstDouble ('-', "qual-filter-slope",0.02); filter_quality_quadr_ = opts.GetFirstDouble ('-', "qual-filter-quadr",0.00); - // Adapter trimming options trim_adapter_ = opts.GetFirstStringVector ('-', "trim-adapter", "ATCACCGACTGCCCATAGAGAGGCTGAGAC"); trim_adapter_cutoff_ = opts.GetFirstDouble ('-', "trim-adapter-cutoff", 16.0); @@ -682,8 +680,7 @@ BaseCallerFilters::BaseCallerFilters(OptArgs& opts, Json::Value &comments_json, // If this flag is set all filters & trimmers will be disabled if (disable_all_filters) { filter_keypass_enabled_ = false; - filter_clonal_enabled_tfs_ = false; - filter_clonal_enabled_ = false; + clonal_opts_.Disable(); filter_residual_enabled_ = false; filter_residual_enabled_tfs_ = false; filter_quality_enabled_ = false; @@ -697,8 +694,6 @@ BaseCallerFilters::BaseCallerFilters(OptArgs& opts, Json::Value &comments_json, printf(" --disable-all-filters %s\n", disable_all_filters ? "on (overrides other options)" : "off"); printf(" --keypass-filter %s\n", filter_keypass_enabled_ ? "on" : "off"); printf(" --min-read-length %d\n", filter_min_read_length_); - printf(" --clonal-filter-solve %s\n", filter_clonal_enabled_ ? "on" : "off"); - printf(" --clonal-filter-tf %s\n", filter_clonal_enabled_tfs_ ? "on" : "off"); printf(" --cr-filter %s\n", filter_residual_enabled_ ? "on" : "off"); printf(" --cr-filter-tf %s\n", filter_residual_enabled_tfs_ ? "on" : "off"); printf(" --cr-filter-max-value %1.3f\n", filter_residual_max_value_); @@ -706,6 +701,7 @@ BaseCallerFilters::BaseCallerFilters(OptArgs& opts, Json::Value &comments_json, printf(" --qual-filter %s\n", filter_quality_enabled_ ? "on" : "off"); printf(" --qual-filter-offset %1.4f\n", filter_quality_offset_); printf(" --qual-filter-slope %1.4f\n", filter_quality_slope_ ); + printf("\n"); printf("Adapter trimming settings\n"); printf(" --trim-adapter "); @@ -764,17 +760,21 @@ BaseCallerFilters::BaseCallerFilters(OptArgs& opts, Json::Value &comments_json, // ---------------------------------------------------------------------------- -void BaseCallerFilters::TrainClonalFilter(const string& output_directory, RawWells& wells, Mask& mask, const PolyclonalFilterOpts & opts) +void BaseCallerFilters::TrainClonalFilter(const string& output_directory, RawWells& wells, Mask& mask) { - if (!filter_clonal_enabled_ and !filter_clonal_enabled_tfs_) + + if (!(clonal_opts_.enable)){ + cout << "Polyclonal filter training disabled." << endl; return; + } + cout << "Polyclonal filter training." << endl; wells.OpenForIncrementalRead(); vector key_ionogram(keys_[0].flows(), keys_[0].flows()+keys_[0].flows_length()); filter_counts counts; int nlib = mask.GetCount(static_cast (MaskLib)); - counts._nsamp = min(nlib, filter_clonal_maxreads_); - make_filter(clonal_population_, counts, mask, wells, key_ionogram, opts); + counts._nsamp = min(nlib, clonal_opts_.filter_clonal_maxreads); + make_filter(clonal_population_, counts, mask, wells, key_ionogram, clonal_opts_); cout << counts << endl; wells.Close(); } @@ -888,20 +888,19 @@ void BaseCallerFilters::SetBkgmodelFailedKeypass(int read_index, ReadFilteringHi // ---------------------------------------------------------------------------- -void BaseCallerFilters::FilterHighPPFAndPolyclonal (int read_index, int read_class, ReadFilteringHistory& filter_history, - const vector& measurements, - const PolyclonalFilterOpts & opts) +void BaseCallerFilters::FilterHighPPFAndPolyclonal (int read_index, int read_class, + ReadFilteringHistory& filter_history, const vector& measurements) { if (filter_history.is_filtered) return; - if (read_class == 0 and !filter_clonal_enabled_) // Filter disabled for library? + if (read_class == 0 and !(clonal_opts_.filter_clonal_enabled_lib)) // Filter disabled for library? return; - if (read_class != 0 and !filter_clonal_enabled_tfs_) // Filter disabled for TFs? + if (read_class != 0 and !(clonal_opts_.filter_clonal_enabled_tfs)) // Filter disabled for TFs? return; - vector::const_iterator first = measurements.begin() + opts.mixed_first_flow; - vector::const_iterator last = measurements.begin() + opts.mixed_last_flow; + vector::const_iterator first = measurements.begin() + clonal_opts_.mixed_first_flow; + vector::const_iterator last = measurements.begin() + clonal_opts_.mixed_last_flow; float ppf = percent_positive(first, last); float ssq = sum_fractional_part(first, last); @@ -911,7 +910,7 @@ void BaseCallerFilters::FilterHighPPFAndPolyclonal (int read_index, int read_cla filter_history.n_bases_after_high_ppf = 0; filter_history.is_filtered = true; } - else if(!clonal_population_.is_clonal(ppf, ssq)) { + else if(!(clonal_opts_.filter_extreme_ppf_only) and !(clonal_population_.is_clonal(ppf, ssq, clonal_opts_.mixed_stringency))) { filter_mask_[read_index] = kFilterPolyclonal; filter_history.n_bases_filtered = 0; filter_history.n_bases_after_polyclonal = 0; @@ -1058,6 +1057,7 @@ void BaseCallerFilters::FilterFailedKeypass(int read_index, int read_class, Read if ((int)sequence.size() >= keys_[read_class].bases_length()) { int base=0; + //while (base < keys_[read_class].bases_length() and isBaseMatch(sequence[base], keys_[read_class].bases()[base])) while (base < keys_[read_class].bases_length() and sequence[base] == keys_[read_class].bases()[base]) ++base; diff --git a/Analysis/BaseCaller/BaseCallerFilters.h b/Analysis/BaseCaller/BaseCallerFilters.h index 9675559f..4473b1cf 100644 --- a/Analysis/BaseCaller/BaseCallerFilters.h +++ b/Analysis/BaseCaller/BaseCallerFilters.h @@ -13,7 +13,8 @@ #include "BaseCallerUtils.h" #include "Mask.h" -#include "mixed.h" +#include "ClonalFilter/polyclonal_filter.h" +#include "ClonalFilter/mixed.h" #include "OptArgs.h" class DPTreephaser; @@ -60,8 +61,7 @@ class BaseCallerFilters { //! @param wells Wells file reader object, source of filter training reads //! @param max_sample_size Max number of reads to sample for training //! @param mask Mask for determining which reads are eligible for training set - //! @param opts User options for polyclonal filter and flows - void TrainClonalFilter(const string& output_directory, RawWells& wells, Mask& mask, const PolyclonalFilterOpts & opts); + void TrainClonalFilter(const string& output_directory, RawWells& wells, Mask& mask); //! @brief Once filtering is complete, transfer filtering outcomes to Mask object. //! @@ -109,8 +109,7 @@ class BaseCallerFilters { //! @param read_index Read index //! @param read_class Read class, 0=library, 1=TFs //! @param measurements Key-normalized flow signal from wells - //! @param opts User options for how filters work - void FilterHighPPFAndPolyclonal (int read_index, int read_class, ReadFilteringHistory& filter_history, const vector& measurements, const PolyclonalFilterOpts & opts); + void FilterHighPPFAndPolyclonal (int read_index, int read_class, ReadFilteringHistory& filter_history, const vector& measurements); //! @brief Trim key sequence and initialize filtered read length //! @param key_length Length of key sequence @@ -243,9 +242,8 @@ class BaseCallerFilters { bool filter_residual_enabled_; //!< Is residual filter enabled for library reads? bool filter_residual_enabled_tfs_; //!< Is residual filter enabled for TFs? double filter_residual_max_value_; //!< Residual filter threshold - bool filter_clonal_enabled_; //!< Is polyclonal filter enabled for library reads? - bool filter_clonal_enabled_tfs_; //!< Is polyclonal filter enabled for TFs? - int filter_clonal_maxreads_; //!< Number of reads to be used for clonal filter training + + PolyclonalFilterOpts clonal_opts_; //! Class to store the clonal filter options clonal_filter clonal_population_; //!< Object implementing clonal filter // Beverly filter diff --git a/Analysis/BaseCaller/BaseCallerParameters.cpp b/Analysis/BaseCaller/BaseCallerParameters.cpp index 280bf66a..9a02ac2e 100644 --- a/Analysis/BaseCaller/BaseCallerParameters.cpp +++ b/Analysis/BaseCaller/BaseCallerParameters.cpp @@ -116,6 +116,7 @@ void BaseCallerParameters::PrintHelp() printf (" --flow-order STRING flow order [retrieved from wells file]\n"); printf (" --run-id STRING read name prefix [hashed input dir name]\n"); printf (" -n,--num-threads INT number of worker threads [2*numcores]\n"); + printf (" --compress-bam BOOL Output compressed / uncompressed BAM [true]\n"); printf (" -f,--flowlimit INT basecall only first n flows [all flows]\n"); printf (" --keynormalizer STRING key normalization algorithm [gain]\n"); printf (" --wells-normalization STRING normalize wells signal and correct for signal bias [off]/on/keyOnly/signalBiasOnly/pinZero\n"); @@ -227,9 +228,11 @@ bool BaseCallerParameters::InitContextVarsFromOptArgs(OptArgs& opts){ context_vars.run_id = opts.GetFirstString ('-', "run-id", default_run_id); num_threads_ = opts.GetFirstInt ('n', "num-threads", max(2*numCores(), 4)); num_bamwriter_threads_ = opts.GetFirstInt ('-', "num-threads-bamwriter", 0); + compress_output_bam_ = opts.GetFirstBoolean('-', "compress-bam", true); context_vars.flow_signals_type = opts.GetFirstString ('-', "flow-signals-type", "none"); context_vars.only_process_unfiltered_set = opts.GetFirstBoolean('-', "only-process-unfiltered-set", false); + context_vars.flow_predictors_ = opts.GetFirstBoolean('-', "flow-predictors", false); // Treephaser options #if defined( __SSE3__ ) @@ -245,6 +248,7 @@ bool BaseCallerParameters::InitContextVarsFromOptArgs(OptArgs& opts){ context_vars.wells_norm_method = opts.GetFirstString ('-', "wells-normalization", "off"); context_vars.just_phase_estimation = opts.GetFirstBoolean('-', "just-phase-estimation", false); context_vars.calibrate_TFs = opts.GetFirstBoolean('-', "calibrate-tfs", false); + context_vars.trim_zm = opts.GetFirstBoolean('-', "trim-zm", true); // debug options context_vars.debug_normalization_bam = opts.GetFirstBoolean ('-', "debug-normalization-bam", false); @@ -343,7 +347,9 @@ bool BaseCallerParameters::SetBaseCallerContextVars(BaseCallerContext & bc) bc.skip_droop = context_vars.skip_droop; bc.skip_recal_during_norm = context_vars.skip_recal_during_norm; bc.calibrate_TFs = context_vars.calibrate_TFs; + bc.trim_zm = context_vars.trim_zm; + bc.flow_predictors_ = context_vars.flow_predictors_; // debug options bc.debug_normalization_bam = context_vars.debug_normalization_bam; return true; diff --git a/Analysis/BaseCaller/BaseCallerParameters.h b/Analysis/BaseCaller/BaseCallerParameters.h index baea25af..4c8d7f22 100644 --- a/Analysis/BaseCaller/BaseCallerParameters.h +++ b/Analysis/BaseCaller/BaseCallerParameters.h @@ -105,6 +105,7 @@ struct BCcontextVars { string run_id; //!< Run ID string, prepended to each read name bool process_tfs; //!< If set to false, TF-related BAM will not be generated bool only_process_unfiltered_set; + bool flow_predictors_; //!< If set to true, use flow-based predictor for Quality Score prediction string flow_signals_type; //!< The flow signal type: "default" - Normalized and phased, // "wells" - Raw values (unnormalized and not dephased), // "key-normalized" - Key normalized and not dephased, @@ -122,6 +123,7 @@ struct BCcontextVars { bool debug_normalization_bam;//!< Switch to output debug data to the bam file bool just_phase_estimation; //!< BaseCaller will only do phase estimation and nothing else bool calibrate_TFs; //!< Switch to apply calibration to TFs + bool trim_zm; //!< Trim the ZM tag when writing it to the bam file bool options_set; //!< Flag whether options have been read to ensure order }; @@ -143,6 +145,7 @@ struct BaseCallerContext { string flow_signals_type; //!< The flow signal type: "default" - Normalized and phased, "wells" - Raw values (unnormalized and not dephased), "key-normalized" - Key normalized and not dephased, "adaptive-normalized" - Adaptive normalized and not dephased, and "unclipped" - Normalized and phased but unclipped. string output_directory; //!< Root directory for all output files string wells_norm_method; //!< Normalization method for wells file before any processing + bool flow_predictors_; //!< If set to false, TF-related BAM will not be generated bool process_tfs; //!< If set to false, TF-related BAM will not be generated int windowSize; //!< Normalization window size bool have_calibration_panel; //!< Signales the presence of a recalibration panel @@ -153,6 +156,7 @@ struct BaseCallerContext { bool skip_recal_during_norm; //!< Switch to exclude recalibration from the normalization stage bool debug_normalization_bam;//!< Switch to output debug info to the bam file bool calibrate_TFs; //!< Switch to apply calibration to TFs + bool trim_zm; //!< Trim the ZM tag when writing it to the bam file // Important outside entities accessed by BaseCaller ion::ChipSubset chip_subset; //!< Chip coordinate & region handling for Basecaller @@ -166,7 +170,6 @@ struct BaseCallerContext { BarcodeClassifier *calibration_barcodes; //!< Barcode detection for calibration set HistogramCalibration *histogram_calibration; //!< Posterior base call and signal adjustment algorithm LinearCalibrationModel *linear_cal_model; //!< Model estimation of simulated predictions and observed measurements - PolyclonalFilterOpts polyclonal_filter; //!< User options for polyclonal filtering MolecularTagTrimmer *tag_trimmer; //!< Class for tag accounting within read groups // Threaded processing @@ -193,6 +196,7 @@ class BaseCallerParameters { BaseCallerParameters() { num_threads_ = 1; num_bamwriter_threads_ = 1; + compress_output_bam_ = true; bc_files.options_set = false; sampling_opts.options_set = false; context_vars.options_set = false; @@ -212,6 +216,8 @@ class BaseCallerParameters { bool JustPhaseEstimation() { return context_vars.just_phase_estimation; }; + bool CompressOutputBam() { return compress_output_bam_; }; + const BaseCallerFiles & GetFiles() const { if (not bc_files.options_set){ @@ -234,8 +240,11 @@ class BaseCallerParameters { int NumBamWriterThreads() const { return num_bamwriter_threads_; }; private: + int num_threads_; //!< Number of worker threads to do base calling int num_bamwriter_threads_; //!< Number of threads one bam writer object uses + bool compress_output_bam_; //!< Switch to output compressed / uncompressed BAM + BaseCallerFiles bc_files; BCcontextVars context_vars; BCwellSampling sampling_opts; diff --git a/Analysis/BaseCaller/DPTreephaser.cpp b/Analysis/BaseCaller/DPTreephaser.cpp index 96277f62..33284c61 100644 --- a/Analysis/BaseCaller/DPTreephaser.cpp +++ b/Analysis/BaseCaller/DPTreephaser.cpp @@ -859,16 +859,38 @@ void DPTreephaser::AdvanceStateInPlace(TreephaserPath *state, char nuc, int max_ //------------------------------------------------------------------------- void DPTreephaser::Simulate(BasecallerRead& data, int max_flows,bool state_inphase) +{ + SimulateStateMatrix(data, max_flows, state_inphase, NULL, 0.0f); +} + +//------------------------------------------------------------------------- + +void DPTreephaser::SimulateStateMatrix(BasecallerRead& data, int max_flows,bool state_inphase, vector > >* row_linked_state_matrix, float amp_cut_off) { InitializeState(&path_[0]); int recent_flow = 0; float recent_state_inphase = 1.0; + bool is_output_state_matrix = (row_linked_state_matrix != NULL); + if (is_output_state_matrix){ + row_linked_state_matrix->reserve(data.sequence.size()); + } for (vector::iterator nuc = data.sequence.begin(); nuc != data.sequence.end() and path_[0].flow < max_flows; ++nuc) { AdvanceStateInPlace(&path_[0], *nuc, flow_order_.num_flows()); path_[0].sequence.push_back(*nuc); // Needed to simulate diagonal states correctly + if (is_output_state_matrix){ + vector > sparse_matrix_row; + sparse_matrix_row.reserve(path_[0].window_end - path_[0].window_start); + for (int flow = path_[0].window_start; flow < path_[0].window_end; ++flow){ + if (path_[0].state[flow] > amp_cut_off){ + sparse_matrix_row.push_back(pair(flow, path_[0].state[flow])); + } + } + row_linked_state_matrix->push_back(sparse_matrix_row); + } + if (state_inphase and path_[0].flow < max_flows) { for (int iFlow=recent_flow+1; iFlowflow >= flow_order_.num_flows()) { + penalty[nuc] = 25; // Mark for deletion + continue; + } + + if (child->last_hp > kMaxHP) { + penalty[nuc] = 25; // Mark for deletion + continue; + } + + if ((int)parent->sequence.size() >= (2 * flow_order_.num_flows() - 10)) { + penalty[nuc] = 25; // Mark for deletion + continue; + } + + for (int flow = parent->window_start; flow < child->window_end; ++flow) { + float residual = read.normalized_measurements[flow] - child->prediction[flow]; + if (residual <= 0 or flow < child->flow) + penalty[nuc] += residual*residual; + } + } //looping over nucs + + + // find current incorporating base + assert(children[called_nuc]->flow == solution_flow); + + recent_state_inphase = children[called_nuc]->state[solution_flow]; + recent_state_total = 0; + for (int flow = children[called_nuc]->window_start; flow < children[called_nuc]->window_end; ++flow) + recent_state_total += children[called_nuc]->state[flow]; + + // Get delta penalty to next best solution + read.penalty_mismatch[base] = -1; // min delta penalty to earlier base hypothesis + read.penalty_residual[base] = 0; + + if (solution_flow - parent->window_start > 0) + read.penalty_residual[base] = penalty[called_nuc] / (solution_flow - parent->window_start); + + for (int nuc = 0; nuc < 4; ++nuc) { + if (nuc == called_nuc) + continue; + float penalty_mismatch = penalty[called_nuc] - penalty[nuc]; + read.penalty_mismatch[base] = max(read.penalty_mismatch[base], penalty_mismatch); + } + + // Fill out the remaining portion of the prediction + for (int flow = 0; flow < parent->window_start; ++flow) + children[called_nuc]->prediction[flow] = parent->prediction[flow]; + + for (int flow = children[called_nuc]->window_end; flow < flow_order_.num_flows(); ++flow) + children[called_nuc]->prediction[flow] = 0; + + // Called state is the starting point for next base + TreephaserPath *swap = parent; + parent = children[called_nuc]; + children[called_nuc] = swap; + + } + + read.state_inphase[solution_flow] = max(recent_state_inphase, 0.01f); + read.state_total[solution_flow] = max(recent_state_total, 0.01f); + } + + read.prediction.swap(parent->prediction); + +} + + +void DPTreephaser::ComputeQVmetrics_flow(BasecallerRead& read, vector& flow_to_base, const bool flow_predictors_) +{ + //static const char nuc_int_to_char[5] = "ACGT"; + //read.state_total.assign(flow_order_.num_flows(), 1); + + if (read.sequence.empty()) + return; + int sz = (int)read.sequence.size(); + if (flow_predictors_) + sz = flow_order_.num_flows(); + + read.penalty_mismatch.assign(sz, 0); + read.penalty_residual.assign(sz, 0); TreephaserPath *parent = &path_[0]; TreephaserPath *children[4] = { &path_[1], &path_[2], &path_[3], &path_[4] }; @@ -1335,7 +1468,11 @@ void DPTreephaser::ComputeQVmetrics(BasecallerRead& read) // main loop for base calling for (int solution_flow = 0, base = 0; solution_flow < flow_order_.num_flows(); ++solution_flow) { - for (; base < (int)read.sequence.size() and read.sequence[base] == flow_order_[solution_flow]; ++base) { + for (; ; ++base) { + if (flow_predictors_) + break; + else if (not (base < sz and read.sequence[base] == flow_order_[solution_flow])) + break; float penalty[4] = { 0, 0, 0, 0 }; @@ -1419,6 +1556,7 @@ void DPTreephaser::ComputeQVmetrics(BasecallerRead& read) } + // ---------------------------------------------------------------------------- void DPTreephaser::TreephaserPath::Initialize() diff --git a/Analysis/BaseCaller/DPTreephaser.h b/Analysis/BaseCaller/DPTreephaser.h index f047893d..9f1b3598 100644 --- a/Analysis/BaseCaller/DPTreephaser.h +++ b/Analysis/BaseCaller/DPTreephaser.h @@ -43,6 +43,8 @@ struct BasecallerRead { vector state_total; //!< Fraction of live polymerase vector penalty_residual; //!< Absolute score of the called nuc hypothesis vector penalty_mismatch; //!< Score difference to second-best nuc hypothesis + vector penalty_residual_flow; //!< Absolute score of the called nuc hypothesis + vector penalty_mismatch_flow; //!< Score difference to second-best nuc hypothesis // Nuc gain data static constexpr float kZeromerMin = -0.20f; //!< Key flow corrected non-key flow zeromer 3-sigma minimum @@ -130,6 +132,14 @@ class DPTreephaser { //! @param[in] restart_flows Number of flows to simulate, rather than solve void Solve(BasecallerRead& read, int max_flows, int restart_flows = 0); + //! @brief Generate predicted signal and the row-linked sparse state matrix from base sequence + //! @param[in] read.sequence Base sequence + //! @param[out] read.prediction Predicted signal + //! @param[in] max_flows Number of flows to process + //! @param[out] row_linked_state_matrix (*row_linked_state_matrix)[nuc][j].second = the amplitude that read.sequence[nuc] contributes to read.prediction[flow] where flow = *(row_linked_state_matrix)[nuc][j].first + //! @param[in] amp_cut_off Neglect the entry in *row_linked_state_matrix if less than this value. + void SimulateStateMatrix(BasecallerRead& read, int max_flows, bool state_inphase, vector > >* row_linked_state_matrix, float amp_cut_off = 0.0f); + //! @brief Generate predicted signal from base sequence //! @param[in] read.sequence Base sequence //! @param[out] read.prediction Predicted signal @@ -160,7 +170,8 @@ class DPTreephaser { //! @param[out] read.onemer_height Expected 1-mer signal, used for scaling residuals //! @param[out] read.penalty_residual Absolute score of the called nuc hypothesis //! @param[out] read.penalty_mismatch Score difference to second-best nuc hypothesis - void ComputeQVmetrics(BasecallerRead& read); // Computes "oneMerHeight" and "deltaPenalty" + void ComputeQVmetrics(BasecallerRead& read); // Computes "oneMerHeight" and "deltaPenalty" + void ComputeQVmetrics_flow(BasecallerRead& read, vector& flow_to_base, const bool flow_predictors_=false); // Computes "oneMerHeight" and "deltaPenalty" //! @brief Correct for uniform multiplicative scaling //! @param[in] read.prediction Model-predicted signal diff --git a/Analysis/BaseCaller/MolecularTagTrimmer.cpp b/Analysis/BaseCaller/MolecularTagTrimmer.cpp index c5b9cf7e..21191dc8 100644 --- a/Analysis/BaseCaller/MolecularTagTrimmer.cpp +++ b/Analysis/BaseCaller/MolecularTagTrimmer.cpp @@ -33,22 +33,24 @@ MolecularTagTrimmer::MolecularTagTrimmer() void MolecularTagTrimmer::PrintHelp(bool tvc_call) { + string space1_for_tvc = tvc_call? " " : ""; + string space2_for_tvc = tvc_call? " " : ""; + cout << "Molecular tagging options:" << endl; + // Both + cout << " --suppress-mol-tags " << space1_for_tvc << "BOOL" << space2_for_tvc << " Ignore tag information [false]" << endl; + cout << " --tag-trim-method " << space1_for_tvc << "STRING" << space2_for_tvc << " Method to trim tags. Options: {strict-trim, sloppy-trim} [sloppy-trim]" << endl; // TVC only options if (tvc_call) - cout << " --min-tag-fam-size INT Minimum required size of molecular tag family [3]" << endl; - + cout << " --min-tag-fam-size " << space1_for_tvc << "INT" << space2_for_tvc << " Minimum required size of molecular tag family [3]" << endl; // BaseCaller only options else{ cout << " --prefix-mol-tag STRING Structure of prefix molecular tag {ACGTN bases}" << endl; cout << " --suffix-mol-tag STRING Structure of suffix molecular tag {ACGTN bases}" << endl; - cout << " --tag-trim-method STRING Method to trim tags. Options: {strict-trim, sloppy-trim} [sloppy-trim]" << endl; - cout << " --heal-tag-hp-indel Bool Heal hp indel on tags [true]" << endl; + cout << " --heal-tag-hp-indel BOOL Heal hp indel on tags [true]" << endl; + cout << " --tag-filter-method STRING Filter reads based on tags. Options: {need-prefix, need-suffix, need-all} [need-all]" << endl; + cout << endl; } - // Both - cout << " --suppress-mol-tags BOOL Ignore tag information [false]" << endl; - cout << " --tag-filter-method STRING Filter reads based on tags. Options: {need-prefix, need-suffix, need-all} [need-all]" << endl; - cout << endl; } @@ -256,12 +258,12 @@ void MolecularTagTrimmer::PrintOptionValues(bool tvc_call) // Verbose output XXX do not say anything if there are no tags if (num_read_groups_with_tags_ > 0) { cout << "MolecularTagTrimmer settings:" << endl; - cout << " found " << num_read_groups_with_tags_ << " read groups with tags." << endl; - cout << " suppress-mol-tags : " << (suppress_mol_tags_ ? "on" : "off") << endl; + cout << " found " << num_read_groups_with_tags_ << " read groups with tags." << endl; + cout << " suppress-mol-tags : " << (suppress_mol_tags_ ? "on" : "off") << endl; if (not tvc_call) { cout << " heal-tag-hp-indel : " << (heal_tag_hp_indel_? "on": "off") << endl; - cout << " tag-trim-method : "; + cout << " tag-trim-method : "; switch (tag_trim_method_){ case kSloppyTrim : cout << "sloppy-trim" << endl; break; case kStrictTrim : cout << "strict-trim" << endl; break; @@ -571,6 +573,14 @@ string MolecularTagTrimmer::GetPrefixTag (string read_group_name) const // ------------------------------------------------------------------------- +string MolecularTagTrimmer::GetPrefixTag (int read_group_idx) const +{ + string tag = tag_structure_.at(read_group_idx).prefix_mol_tag; + return tag; +} + +// ------------------------------------------------------------------------- + string MolecularTagTrimmer::GetSuffixTag (string read_group_name) const { string tag; @@ -583,6 +593,14 @@ string MolecularTagTrimmer::GetSuffixTag (string read_group_name) const // ------------------------------------------------------------------------- +string MolecularTagTrimmer::GetSuffixTag (int read_group_idx) const +{ + string tag = tag_structure_.at(read_group_idx).suffix_mol_tag; + return tag; +} + +// ------------------------------------------------------------------------- + bool MolecularTagTrimmer::HasTags(string read_group_name) const { bool has_tags = false; diff --git a/Analysis/BaseCaller/MolecularTagTrimmer.h b/Analysis/BaseCaller/MolecularTagTrimmer.h index 8716a744..329ccb50 100644 --- a/Analysis/BaseCaller/MolecularTagTrimmer.h +++ b/Analysis/BaseCaller/MolecularTagTrimmer.h @@ -164,12 +164,15 @@ class MolecularTagTrimmer MolTag GetReadGroupTags (string read_group_name) const; string GetPrefixTag (string read_group_name) const; + string GetPrefixTag (int read_group_idx) const; string GetSuffixTag (string read_group_name) const; + string GetSuffixTag (int read_group_idx) const; bool HasTags(int read_group_idx) const { return read_group_has_tags_.at(read_group_idx); }; bool HasTags(string read_group_name) const; bool HaveTags() const { return (num_read_groups_with_tags_>0); }; + int GetTagTrimMethod() const {return tag_trim_method_; }; }; diff --git a/Analysis/BaseCaller/OrderedDatasetWriter.cpp b/Analysis/BaseCaller/OrderedDatasetWriter.cpp index e04c2f57..26fbb8f9 100644 --- a/Analysis/BaseCaller/OrderedDatasetWriter.cpp +++ b/Analysis/BaseCaller/OrderedDatasetWriter.cpp @@ -28,6 +28,7 @@ OrderedDatasetWriter::OrderedDatasetWriter() num_read_groups_ = 0; num_datasets_ = 0; save_filtered_reads_ = false; + compress_bam_ = true; num_bamwriter_threads_ = 0; pthread_mutex_init(&dropbox_mutex_, NULL); pthread_mutex_init(&write_mutex_, NULL); @@ -46,7 +47,7 @@ OrderedDatasetWriter::~OrderedDatasetWriter() void OrderedDatasetWriter::Open(const string& base_directory, BarcodeDatasets& datasets, int read_class_idx, int num_regions, const ion::FlowOrder& flow_order, const string& key, const vector & bead_adapters, int num_bamwriter_threads, const Json::Value & basecaller_json, vector& comments, - MolecularTagTrimmer& tag_trimmer, bool trim_barcodes) + MolecularTagTrimmer& tag_trimmer, bool trim_barcodes, bool compress_bam) { num_regions_ = num_regions; num_regions_written_ = 0; @@ -61,6 +62,7 @@ void OrderedDatasetWriter::Open(const string& base_directory, BarcodeDatasets& d num_read_groups_ = datasets.num_read_groups(); num_reads_.resize(num_datasets_,0); bam_filename_.resize(num_datasets_); + compress_bam_ = compress_bam; // A negative read group index indicates untrimmed/unfiltered bam files (w. library key) and we save all reads if (read_class_idx < 0) { @@ -314,9 +316,12 @@ void OrderedDatasetWriter::PhysicalWriteRegion(int region) // Open Bam for writing RefVector empty_reference_vector; bam_writer_[target_file_idx] = new BamWriter(); - bam_writer_[target_file_idx]->SetCompressionMode(BamWriter::Compressed); + if (compress_bam_) + bam_writer_[target_file_idx]->SetCompressionMode(BamWriter::Compressed); + else + bam_writer_[target_file_idx]->SetCompressionMode(BamWriter::Uncompressed); bam_writer_[target_file_idx]->SetNumThreads(num_bamwriter_threads_); - //bam_writer_[ds]->SetCompressionMode(BamWriter::Uncompressed); + if (not bam_writer_[target_file_idx]->Open(bam_filename_[target_file_idx], sam_header_[target_file_idx], empty_reference_vector)) { cerr << "BaseCaller IO error: Failed to create bam file " << bam_filename_[target_file_idx] << endl; exit(EXIT_FAILURE); diff --git a/Analysis/BaseCaller/OrderedDatasetWriter.h b/Analysis/BaseCaller/OrderedDatasetWriter.h index 2ece7f7d..7277fb83 100644 --- a/Analysis/BaseCaller/OrderedDatasetWriter.h +++ b/Analysis/BaseCaller/OrderedDatasetWriter.h @@ -182,7 +182,7 @@ class OrderedDatasetWriter { void Open(const string& base_directory, BarcodeDatasets& datasets, int read_class_idx, int num_regions, const ion::FlowOrder& flow_order, const string& key, const vector & bead_adapters, int num_bamwriter_threads, const Json::Value & basecaller_json, vector& comments, - MolecularTagTrimmer& tag_trimmer, bool trim_barcodes); + MolecularTagTrimmer& tag_trimmer, bool trim_barcodes, bool compress_bam); //! @brief Drop off a region-worth of reads for writing. Write opportunistically. //! @param region Index of the region being dropped off. @@ -230,6 +230,7 @@ class OrderedDatasetWriter { vector bam_filename_; bool save_filtered_reads_; + bool compress_bam_; int num_bamwriter_threads_; vector read_group_num_Q20_bases_; //!< Number of >=Q20 bases written per read group diff --git a/Analysis/BaseCaller/PerBaseQual.cpp b/Analysis/BaseCaller/PerBaseQual.cpp index cb83d2c4..499dada7 100644 --- a/Analysis/BaseCaller/PerBaseQual.cpp +++ b/Analysis/BaseCaller/PerBaseQual.cpp @@ -424,15 +424,17 @@ uint8_t PerBaseQual::CalculatePerBaseScore(float* pred) const // Predictor 2 - Local noise/flowalign - Maximum residual within +-1 BASE void PerBaseQual::PredictorLocalNoise(vector& local_noise, int max_base, const vector& base_to_flow, - const vector& normalized_measurements, const vector& prediction) + const vector& normalized_measurements, const vector& prediction, const bool flow_predictors_) { int num_bases = base_to_flow.size(); for (int base = 0; base < max_base; ++base) { int val1 = max(base - 1, 0); - int val2 = min(base + 1, num_bases - 1); + //int val2 = min(base + 1, num_bases - 1); + int val2 = flow_predictors_ ? min(base+1, max_base-1) : min(base+1, num_bases-1); float noise = 0; for (int j = val1; j <= val2; ++j) { - noise = max(noise, fabsf(normalized_measurements[base_to_flow[j]] - prediction[base_to_flow[j]])); + int jj = flow_predictors_ ? j : base_to_flow[j]; + noise = max(noise, fabsf(normalized_measurements[jj] - prediction[jj])); } local_noise[base] = noise; } @@ -443,7 +445,7 @@ void PerBaseQual::PredictorLocalNoise(vector& local_noise, int max_base, // -(m_1 - m_0 - s_1 - s_0)/m_1 void PerBaseQual::PredictorNoiseOverlap(vector& minus_noise_overlap, int max_base, - const vector& normalized_measurements, const vector& prediction) + const vector& normalized_measurements, const vector& prediction, const bool flow_predictors_) { // 0-mer and 1-mer overlap // define 0-mer and 1-mer interval @@ -513,37 +515,43 @@ void PerBaseQual::PredictorNoiseOverlap(vector& minus_noise_overlap, int // Predictor 4 - Transformed homopolymer length -void PerBaseQual::PredictorHomopolymerRank(vector& homopolymer_rank, int max_base, const vector& sequence) +void PerBaseQual::PredictorHomopolymerRank(vector& homopolymer_rank, int max_base, const vector& sequence, vector& homopolymer_rank_flow, const vector& flow_to_base, int flow_predictors_) { - int hp_length = 0; - for (int base = 0; base < max_base; ++base) { + int hp_length = 0; + for (int base = 0; base < max_base; ++base) { hp_length++; // HP 1114 homopolymer_rank[base] = 1; if (sequence[base] != sequence[base+1] or (base+2) == max_base) { homopolymer_rank[base] = hp_length; hp_length = 0; + } + } + int nFlows = flow_to_base.size(); + for (int flow=0; flow=0 && base& neighborhood_noise, int max_base, const vector& base_to_flow, - const vector& normalized_measurements, const vector& prediction) + const vector& normalized_measurements, const vector& prediction, const bool flow_predictors_) { int num_bases = base_to_flow.size(); for (int base = 0; base < max_base; ++base) { int radius = 5; // protect at start/end of read int val1 = max(base-radius, 0); - int val2 = min(base+radius, num_bases-1); + int val2 = flow_predictors_ ? min(base+radius, max_base-1) : min(base+radius, num_bases-1); float noise = 0; int count = 0; for (int j = val1; j <= val2; j++) { - noise += fabsf(normalized_measurements[base_to_flow[j]] - prediction[base_to_flow[j]]); + int jj = flow_predictors_ ? j : base_to_flow[j]; + noise += fabsf(normalized_measurements[jj] - prediction[jj]); count++; } if (count) @@ -557,7 +565,7 @@ void PerBaseQual::PredictorNeighborhoodNoise(vector& neighborhood_noise, // Candidate predictor based on Beverly filter void PerBaseQual::PredictorBeverlyEvents(vector& beverly_events, int max_base, const vector& base_to_flow, - const vector& scaled_residual) + const vector& scaled_residual, const bool flow_predictors_) { const static int flow_window_radius = 10; @@ -568,9 +576,7 @@ void PerBaseQual::PredictorBeverlyEvents(vector& beverly_events, int max_ int num_beverly_events = 0; for (int base = 0; base < max_base; ++base) { - - int window_center_flow = base_to_flow[base]; - + int window_center_flow = flow_predictors_ ? base : base_to_flow[base]; // Advance window start while (window_start_flow < window_center_flow+flow_window_radius) { int hp_length = 0; @@ -610,60 +616,78 @@ void PerBaseQual::GenerateBaseQualities(const string& read_name, int num_bases, const vector &predictor1, const vector &predictor2, const vector &predictor3, const vector &predictor4, const vector &predictor5, const vector &predictor6, const vector& base_to_flow, vector &quality, - const vector &candidate1, const vector &candidate2, const vector &candidate3) + const vector &candidate1, const vector &candidate2, const vector &candidate3, + const vector &predictor1_flow, const vector &predictor5_flow, const vector &predictor4_flow, + const vector& flow_to_base, const bool flow_predictors_) { if (num_bases == 0) return; //! \todo This is a temporary fix for very long sequences that are sometimes generated by the basecaller - int max_eligible_base = min(num_bases, (int)(0.75*num_flows) + 1); - quality.clear(); + int last_base_to_flow = base_to_flow.back(); + int max_eligible_flow = (int)(0.75*num_flows) + 1; + max_eligible_flow = min(max_eligible_flow,last_base_to_flow); + //save_predictors_ = false; // debugging only + int max_eligible_base = flow_predictors_ ? max_eligible_flow : min(num_bases, max_eligible_flow); + //int max_eligible_base = min(num_bases, max_eligible_flow); // avoid out of range in debugging + quality.clear(); stringstream predictor_dump_block; for (int base = 0; base < max_eligible_base; base++) { // first 4 bases are the keys TCAG - float pred[kNumPredictors]; - pred[1] = predictor2[base]; - pred[2] = predictor3[base]; - pred[3] = predictor4[base]; - + pred[1] = predictor2[base]; // P2: local noise + pred[2] = predictor3[base]; // P3: high-residual events + int base_or_flow = flow_predictors_ ? base : base_to_flow[base]; + /* if (save_predictors_) { // the following lines are only for predictor_dump_block // they are not the same in new QvTables - pred[0] = predictor1[base]; - pred[4] = predictor5[base]; - pred[5] = predictor6[base]; + pred[0] = flow_predictors_ ? predictor1_flow[base] : predictor1[base]; // P1: penalty residual + pred[3] = flow_predictors_ ? predictor4_flow[base] : predictor4[base]; // P4: hp + pred[4] = flow_predictors_ ? predictor5_flow[base] : predictor5[base]; // P5: penalty mismatch + pred[5] = predictor6[base]; // P6: neighborhood noise predictor_dump_block << read_name << " " << base << " "; for (int k = 0; k < kNumPredictors; ++k) predictor_dump_block << pred[k] << " "; - predictor_dump_block << candidate1[base_to_flow[base]] << " "; - predictor_dump_block << candidate2[base_to_flow[base]] << " "; - predictor_dump_block << candidate3[base_to_flow[base]] << " "; - predictor_dump_block << base_to_flow[base] << endl; + predictor_dump_block << candidate1[base_or_flow] << " "; + predictor_dump_block << candidate2[base_or_flow] << " "; + predictor_dump_block << candidate3[base_or_flow] << " "; + if (flow_predictors_) { + int always_base = flow_predictors_ ? flow_to_base[base] : base; + predictor_dump_block << always_base << endl; // could be -1 + } else { + int always_flow = flow_predictors_ ? base : base_to_flow[base]; + predictor_dump_block << always_flow << endl; // cannot get flow if base=-1 + } + //predictor_dump_block << base_to_flow[base] << endl; } + */ // v3.4: p1,2,3,4,6,9 // the real predictors used in the QvTable pred[0] = transform_P1(predictor1[base]); - //pred[1] = transform_P2(predictor2[base]); // no transformation might help only if no Recalibration + pred[3] = predictor4[base]; // P4: hp + //pred[3] = flow_predictors_ ? predictor4_flow[base] : predictor4[base]; // P4: hp pred[4] = transform_P6(predictor6[base]); + //pred[1] = transform_P2(predictor2[base]); // no transformation might help only if no Recalibration //pred[5] = transform_P8(candidate2[base_to_flow[base]]); - pred[5] = transform_P9(candidate3[base_to_flow[base]]); + pred[5] = candidate3[base_or_flow]; + pred[5] = transform_P9(pred[5]); // v3.0: p1,2,3,4,5,6 //pred[0] = predictor1[base]; //pred[0] = transform_P1(predictor1[base]); //pred[4] = predictor5[base]; //pred[5] = predictor6[base]; - quality.push_back(CalculatePerBaseScore(pred)); } for (int base = max_eligible_base; base < num_bases; base++) quality.push_back(kMinQuality); + /* if (save_predictors_) { predictor_dump_block.flush(); pthread_mutex_lock(&predictor_mutex_); @@ -671,9 +695,70 @@ void PerBaseQual::GenerateBaseQualities(const string& read_name, int num_bases, predictor_dump_.flush(); pthread_mutex_unlock(&predictor_mutex_); } + */ } +void PerBaseQual::DumpPredictors(const string& read_name, int num_bases, int num_flows, + const vector &predictor1, const vector &predictor2, const vector &predictor3, + const vector &predictor4, const vector &predictor5, const vector &predictor6, + const vector& base_to_flow, vector &quality, + const vector &candidate1, const vector &candidate2, const vector &candidate3, + const vector &predictor1_flow, const vector &predictor5_flow, const vector &predictor4_flow, + const vector& flow_to_base, const bool flow_predictors_) +{ + + if (num_bases == 0) + return; + + //! \todo This is a temporary fix for very long sequences that are sometimes generated by the basecaller + int last_base_to_flow = base_to_flow.back(); + int max_eligible_flow = (int)(0.75*num_flows) + 1; + max_eligible_flow = min(max_eligible_flow,last_base_to_flow); + //save_predictors_ = false; // debugging only + int max_eligible_base = flow_predictors_ ? max_eligible_flow : min(num_bases, max_eligible_flow); + //int max_eligible_base = min(num_bases, max_eligible_flow); // avoid out of range in debugging + + stringstream predictor_dump_block; + + for (int base = 0; base < max_eligible_base; base++) { // first 4 bases are the keys TCAG + float pred[kNumPredictors]; + pred[1] = predictor2[base]; // P2: local noise + pred[2] = predictor3[base]; // P3: high-residual events + int always_flow = flow_predictors_ ? base : base_to_flow[base]; + //if (save_predictors_) { + // the following lines are only for predictor_dump_block + // they are not the same in new QvTables + pred[0] = flow_predictors_ ? predictor1_flow[base] : predictor1[base]; // P1: penalty residual + pred[3] = flow_predictors_ ? predictor4_flow[base] : predictor4[base]; // P4: hp + pred[4] = flow_predictors_ ? predictor5_flow[base] : predictor5[base]; // P5: penalty mismatch + pred[5] = predictor6[base]; // P6: neighborhood noise + + predictor_dump_block << read_name << " " << base << " "; + for (int k = 0; k < kNumPredictors; ++k) + predictor_dump_block << pred[k] << " "; + predictor_dump_block << candidate1[always_flow] << " "; + predictor_dump_block << candidate2[always_flow] << " "; + predictor_dump_block << candidate3[always_flow] << " "; + if (flow_predictors_) { + int always_base = flow_predictors_ ? flow_to_base[base] : base; + predictor_dump_block << always_base << endl; // could be -1 + } else { + predictor_dump_block << always_flow << endl; // cannot get flow if base=-1 + } + //predictor_dump_block << base_to_flow[base] << endl; + //} + } + + //if (save_predictors_) { + predictor_dump_block.flush(); + pthread_mutex_lock(&predictor_mutex_); + predictor_dump_ << predictor_dump_block.str(); + predictor_dump_.flush(); + pthread_mutex_unlock(&predictor_mutex_); + //} +} + float PerBaseQual::transform_P1(float p) { diff --git a/Analysis/BaseCaller/PerBaseQual.h b/Analysis/BaseCaller/PerBaseQual.h index 25f1176f..c10c020c 100644 --- a/Analysis/BaseCaller/PerBaseQual.h +++ b/Analysis/BaseCaller/PerBaseQual.h @@ -60,7 +60,17 @@ class PerBaseQual { const vector &predictor1, const vector &predictor2, const vector &predictor3, const vector &predictor4, const vector &predictor5, const vector &predictor6, const vector& base_to_flow, vector &quality, - const vector &candidate1, const vector &candidate2, const vector &candidate3); + const vector &candidate1, const vector &candidate2, const vector &candidate3, + const vector &predictor1_flow, const vector &predictor5_flow, const vector &predictor4_flow, + const vector& flow_to_base, const bool flow_predictors_=false); + + void DumpPredictors(const string& read_name, int num_bases, int num_flows, + const vector &predictor1, const vector &predictor2, const vector &predictor3, + const vector &predictor4, const vector &predictor5, const vector &predictor6, + const vector& base_to_flow, vector &quality, + const vector &candidate1, const vector &candidate2, const vector &candidate3, + const vector &predictor1_flow, const vector &predictor5_flow, const vector &predictor4_flow, + const vector& flow_to_base, const bool flow_predictors_=false); //! @brief Calculate Local Noise predictor for all bases in a read //! @param[out] local_noise Local Noise predictor @@ -69,7 +79,7 @@ class PerBaseQual { //! @param[in] normalized_measurements Normalized flow signal from wells file //! @param[in] prediction Model-predicted flow signal static void PredictorLocalNoise(vector& local_noise, int max_base, const vector& base_to_flow, - const vector& normalized_measurements, const vector& prediction); + const vector& normalized_measurements, const vector& prediction, const bool flow_predictors_); //! @brief Calculate Noise Overlap predictor shared by all bases in a read //! @param[out] minus_noise_overlap Noise Overlap predictor @@ -78,13 +88,13 @@ class PerBaseQual { //! @param[in] prediction Model-predicted flow signal //! @return Noise Overlap predictor static void PredictorNoiseOverlap(vector& minus_noise_overlap, int max_base, - const vector& normalized_measurements, const vector& prediction); + const vector& normalized_measurements, const vector& prediction, const bool flow_predictors_); //! @brief Calculate Homopolymer Rank predictor for all bases in a read //! @param[out] homopolymer_rank Homopolymer Rank predictor //! @param[in] max_base Number of bases for which predictor should be calculated //! @param[in] sequence Called bases - static void PredictorHomopolymerRank(vector& homopolymer_rank, int max_base, const vector& sequence); + static void PredictorHomopolymerRank(vector& homopolymer_rank, int max_base, const vector& sequence, vector& homopolymer_rank_flow, const vector& flow_to_base, int flow_predictors_=false); //! @brief Calculate Neighborhood Noise predictor for all bases in a read //! @param[out] neighborhood_noise Neighborhood Noise predictor @@ -93,12 +103,13 @@ class PerBaseQual { //! @param[in] normalized_measurements Normalized flow signal from wells file //! @param[in] prediction Model-predicted flow signal static void PredictorNeighborhoodNoise(vector& neighborhood_noise, int max_base, const vector& base_to_flow, - const vector& normalized_measurements, const vector& prediction); + const vector& normalized_measurements, const vector& prediction, const bool flow_predictors_); static void PredictorBeverlyEvents(vector& beverly_events, int max_base, const vector& base_to_flow, - const vector& scaled_residual); + const vector& scaled_residual, const bool flow_predictors_); + bool toSavePredictors() {return (save_predictors_ ? true:false);} protected: diff --git a/Analysis/BaseCaller/TreephaserSSE.cpp b/Analysis/BaseCaller/TreephaserSSE.cpp index 80721831..a774f795 100644 --- a/Analysis/BaseCaller/TreephaserSSE.cpp +++ b/Analysis/BaseCaller/TreephaserSSE.cpp @@ -1484,15 +1484,15 @@ void TreephaserSSE::WindowedNormalize(BasecallerRead& read, int num_steps) void TreephaserSSE::ComputeQVmetrics(BasecallerRead& read) { static const char nuc_int_to_char[5] = "ACGT"; - - read.state_inphase.assign(flow_order_.num_flows(), 1); - read.state_total.assign(flow_order_.num_flows(), 1); + int num_flows = flow_order_.num_flows(); + read.state_inphase.assign(num_flows, 1); + read.state_total.assign(num_flows, 1); if (read.sequence.empty()) return; - - read.penalty_mismatch.assign(read.sequence.size(), 0); - read.penalty_residual.assign(read.sequence.size(), 0); + int num_bases = read.sequence.size(); + read.penalty_mismatch.assign(num_bases, 0); + read.penalty_residual.assign(num_bases, 0); PathRec RESTRICT_PTR parent = sv_PathPtr[0]; PathRec RESTRICT_PTR children[4] = {sv_PathPtr[1], sv_PathPtr[2], sv_PathPtr[3], sv_PathPtr[4]}; @@ -1512,98 +1512,228 @@ void TreephaserSSE::ComputeQVmetrics(BasecallerRead& read) float recent_state_total = 1; // main loop for base calling - for (int solution_flow = 0, base = 0; solution_flow < flow_order_.num_flows(); ++solution_flow) { - for (; base < (int)read.sequence.size() and read.sequence[base] == flow_order_[solution_flow]; ++base) { - - float penalty[4] = { 0, 0, 0, 0 }; - - int called_nuc = -1; - - if(recalibrate_predictions_) { - parent->calib_A[parent->flow] = (*As_).at(parent->flow).at(flow_order_.int_at(parent->flow)).at(parent->last_hp); - parent->calib_B[parent->flow] = (*Bs_).at(parent->flow).at(flow_order_.int_at(parent->flow)).at(parent->last_hp); - } - - // compute child path flow states, predicted signal,negative and positive penalties - advanceState4(parent, flow_order_.num_flows()); - - for(int nuc = 0; nuc < 4; ++nuc) { - PathRec RESTRICT_PTR child = children[nuc]; - - if (nuc_int_to_char[nuc] == flow_order_[solution_flow]) - called_nuc = nuc; - - child->flow = min(ad_Idx[nuc], flow_order_.num_flows()); - child->window_end = min(ad_End[nuc], flow_order_.num_flows()); - child->window_start = min(ad_Beg[nuc], child->window_end); + for (int solution_flow = 0, base = 0; solution_flow < num_flows; ++solution_flow) { + for (; basecalib_A[parent->flow] = (*As_).at(parent->flow).at(flow_order_.int_at(parent->flow)).at(parent->last_hp); + parent->calib_B[parent->flow] = (*Bs_).at(parent->flow).at(flow_order_.int_at(parent->flow)).at(parent->last_hp); + } + // compute child path flow states, predicted signal,negative and positive penalties + advanceState4(parent, num_flows); + + float penalty[4] = { 0, 0, 0, 0 }; + int called_nuc = -1; + for(int nuc = 0; nuc < 4; ++nuc) { + PathRec RESTRICT_PTR child = children[nuc]; + if (nuc_int_to_char[nuc] == flow_order_[solution_flow]) + called_nuc = nuc; + child->flow = min(ad_Idx[nuc], num_flows); + child->window_end = min(ad_End[nuc], num_flows); + child->window_start = min(ad_Beg[nuc], child->window_end); + // Apply easy termination rules + if (child->flow >= num_flows || parent->last_hp >= MAX_HPXLEN ) { + penalty[nuc] = 25; // Mark for deletion + continue; + } + // pointer in the ad_Buf buffer pointing at the running sum of positive residuals at start of parent window + char RESTRICT_PTR pn = ad_Buf+nuc*4+(AD_NRES_OFS-16)-parent->window_start*16; + // sum of squared residuals for positive residuals for flows < child->flow + float penPar = *((float*)(pn+child->flow*16+(AD_PRES_OFS-AD_NRES_OFS))); + // sum of squared residuals for negative residuals for flows < child->window_end + float penNeg = *((float*)(pn+child->window_end*16)); + penalty[nuc] = penPar + penNeg; + } + // find current incorporating base + int test_nuc = nuc_char_to_int(flow_order_[solution_flow]); + assert(called_nuc==test_nuc); + assert(called_nuc > -1); + assert(children[called_nuc]->flow == solution_flow); + PathRec RESTRICT_PTR childToKeep = children[called_nuc]; + //copy + char RESTRICT_PTR p = ad_Buf+ called_nuc*4 + AD_STATE_OFS; + recent_state_total = 0; + for(int i = parent->window_start, j = 0, e = childToKeep->window_end; i < e; ++i, j += 16) { + childToKeep->state[i] = *((float*)(p+j)); + childToKeep->pred[i] = *((float*)(p+j+(AD_PRED_OFS-AD_STATE_OFS))); + recent_state_total += childToKeep->state[i]; + } + //sse implementation with aligned memory; no gain as the number of elements to be summed up is small + //recent_state_total = vecSumSSE(state_Buf, countStates); + + copySSE(childToKeep->pred, parent->pred, parent->window_start << 2); + if (childToKeep->flow == parent->flow) + childToKeep->last_hp = parent->last_hp = min(parent->last_hp+1, MAX_HPXLEN); + else + childToKeep->last_hp = 1; + + recent_state_inphase = childToKeep->state[solution_flow]; + // Get delta penalty to next best solution + read.penalty_mismatch[base] = -1; // min delta penalty to earlier base hypothesis + read.penalty_residual[base] = 0; + if (solution_flow - parent->window_start > 0) + read.penalty_residual[base] = penalty[called_nuc] / (solution_flow - parent->window_start); + + for (int nuc = 0; nuc < 4; ++nuc) { + if (nuc == called_nuc) + continue; + float penalty_mismatch = penalty[called_nuc] - penalty[nuc]; + read.penalty_mismatch[base] = max(read.penalty_mismatch[base], penalty_mismatch); + } - // Apply easy termination rules - if (child->flow >= flow_order_.num_flows() || parent->last_hp >= MAX_HPXLEN ) { - penalty[nuc] = 25; // Mark for deletion - continue; + // Called state is the starting point for next base + PathRec RESTRICT_PTR swap = parent; + parent = children[called_nuc]; + children[called_nuc] = swap; } + read.state_inphase[solution_flow] = max(recent_state_inphase, 0.01f); + read.state_total[solution_flow] = max(recent_state_total, 0.01f); + } - // pointer in the ad_Buf buffer pointing at the running sum of positive residuals at start of parent window - char RESTRICT_PTR pn = ad_Buf+nuc*4+(AD_NRES_OFS-16)-parent->window_start*16; - - // sum of squared residuals for positive residuals for flows < child->flow - float penPar = *((float*)(pn+child->flow*16+(AD_PRES_OFS-AD_NRES_OFS))); - - // sum of squared residuals for negative residuals for flows < child->window_end - float penNeg = *((float*)(pn+child->window_end*16)); - - penalty[nuc] = penPar + penNeg; - } - - // find current incorporating base - assert(called_nuc > -1); - assert(children[called_nuc]->flow == solution_flow); - - PathRec RESTRICT_PTR childToKeep = children[called_nuc]; - //copy - char RESTRICT_PTR p = ad_Buf+ called_nuc*4 + AD_STATE_OFS; - - recent_state_total = 0; - for(int i = parent->window_start, j = 0, e = childToKeep->window_end; i < e; ++i, j += 16) { - childToKeep->state[i] = *((float*)(p+j)); - childToKeep->pred[i] = *((float*)(p+j+(AD_PRED_OFS-AD_STATE_OFS))); - recent_state_total += childToKeep->state[i]; - } - //sse implementation with aligned memory; no gain as the number of elements to be summed up is small -// recent_state_total = vecSumSSE(state_Buf, countStates); - - copySSE(childToKeep->pred, parent->pred, parent->window_start << 2); + if(recalibrate_predictions_) { + RecalibratePredictions(parent); + ResetRecalibrationStructures(num_flows_); + } + setZeroSSE(&read.prediction[0], num_flows_*sizeof(float)); + copySSE(&read.prediction[0], parent->pred, parent->window_end*sizeof(float)); +} - if (childToKeep->flow == parent->flow) - childToKeep->last_hp = parent->last_hp = min(parent->last_hp+1, MAX_HPXLEN); - else - childToKeep->last_hp = 1; - recent_state_inphase = childToKeep->state[solution_flow]; +void TreephaserSSE::ComputeQVmetrics_flow(BasecallerRead& read, vector& flow_to_base, const bool flow_predictors_) +{ + static const char nuc_int_to_char[5] = "ACGT"; + int num_flows = flow_order_.num_flows(); + read.state_inphase.assign(num_flows, 1); + read.state_total.assign(num_flows, 1); - // Get delta penalty to next best solution - read.penalty_mismatch[base] = -1; // min delta penalty to earlier base hypothesis - read.penalty_residual[base] = 0; + if (read.sequence.empty()) + return; + int num_bases = read.sequence.size(); + read.penalty_mismatch.assign(num_bases, 0); + read.penalty_residual.assign(num_bases, 0); - if (solution_flow - parent->window_start > 0) - read.penalty_residual[base] = penalty[called_nuc] / (solution_flow - parent->window_start); + PathRec RESTRICT_PTR parent = sv_PathPtr[0]; + PathRec RESTRICT_PTR children[4] = {sv_PathPtr[1], sv_PathPtr[2], sv_PathPtr[3], sv_PathPtr[4]}; + parent->flow = 0; + parent->window_start = 0; + parent->window_end = 1; + parent->res = 0.0f; + parent->metr = 0.0f; + parent->flowMetr = 0.0f; + parent->dotCnt = 0; + parent->state[0] = 1.0f; + parent->sequence_length = 0; + parent->last_hp = 0; + parent->pred[0] = 0.0f; - for (int nuc = 0; nuc < 4; ++nuc) { - if (nuc == called_nuc) - continue; - float penalty_mismatch = penalty[called_nuc] - penalty[nuc]; - read.penalty_mismatch[base] = max(read.penalty_mismatch[base], penalty_mismatch); - } + float recent_state_inphase = 1; + float recent_state_total = 1; - // Called state is the starting point for next base - PathRec RESTRICT_PTR swap = parent; - parent = children[called_nuc]; - children[called_nuc] = swap; - } + // main loop for base calling + for (int solution_flow = 0, base = 0; solution_flow < num_flows; ++solution_flow) { + for (; basecalib_A[parent->flow] = (*As_).at(parent->flow).at(flow_order_.int_at(parent->flow)).at(parent->last_hp); + parent->calib_B[parent->flow] = (*Bs_).at(parent->flow).at(flow_order_.int_at(parent->flow)).at(parent->last_hp); + } + // compute child path flow states, predicted signal,negative and positive penalties + advanceState4(parent, num_flows); + + float penalty[4] = { 0, 0, 0, 0 }; + int called_nuc = -1; + for(int nuc = 0; nuc < 4; ++nuc) { + PathRec RESTRICT_PTR child = children[nuc]; + if (nuc_int_to_char[nuc] == flow_order_[solution_flow]) + called_nuc = nuc; + child->flow = min(ad_Idx[nuc], num_flows); + child->window_end = min(ad_End[nuc], num_flows); + child->window_start = min(ad_Beg[nuc], child->window_end); + // Apply easy termination rules + if (child->flow >= num_flows || parent->last_hp >= MAX_HPXLEN ) { + penalty[nuc] = 25; // Mark for deletion + continue; + } + // pointer in the ad_Buf buffer pointing at the running sum of positive residuals at start of parent window + char RESTRICT_PTR pn = ad_Buf+nuc*4+(AD_NRES_OFS-16)-parent->window_start*16; + // sum of squared residuals for positive residuals for flows < child->flow + float penPar = *((float*)(pn+child->flow*16+(AD_PRES_OFS-AD_NRES_OFS))); + // sum of squared residuals for negative residuals for flows < child->window_end + float penNeg = *((float*)(pn+child->window_end*16)); + penalty[nuc] = penPar + penNeg; + } + // find current incorporating base + int test_nuc = nuc_char_to_int(flow_order_[solution_flow]); + assert(called_nuc==test_nuc); + assert(called_nuc > -1); + assert(children[called_nuc]->flow == solution_flow); + PathRec RESTRICT_PTR childToKeep = children[called_nuc]; + //copy + char RESTRICT_PTR p = ad_Buf+ called_nuc*4 + AD_STATE_OFS; + recent_state_total = 0; + for(int i = parent->window_start, j = 0, e = childToKeep->window_end; i < e; ++i, j += 16) { + childToKeep->state[i] = *((float*)(p+j)); + childToKeep->pred[i] = *((float*)(p+j+(AD_PRED_OFS-AD_STATE_OFS))); + recent_state_total += childToKeep->state[i]; + } + //sse implementation with aligned memory; no gain as the number of elements to be summed up is small + //recent_state_total = vecSumSSE(state_Buf, countStates); + + copySSE(childToKeep->pred, parent->pred, parent->window_start << 2); + if (childToKeep->flow == parent->flow) + childToKeep->last_hp = parent->last_hp = min(parent->last_hp+1, MAX_HPXLEN); + else + childToKeep->last_hp = 1; + + recent_state_inphase = childToKeep->state[solution_flow]; + // Get delta penalty to next best solution + read.penalty_mismatch[base] = -1; // min delta penalty to earlier base hypothesis + read.penalty_residual[base] = 0; + if (solution_flow - parent->window_start > 0) + read.penalty_residual[base] = penalty[called_nuc] / (solution_flow - parent->window_start); + + for (int nuc = 0; nuc < 4; ++nuc) { + if (nuc == called_nuc) + continue; + float penalty_mismatch = penalty[called_nuc] - penalty[nuc]; + read.penalty_mismatch[base] = max(read.penalty_mismatch[base], penalty_mismatch); + } + // Called state is the starting point for next base + PathRec RESTRICT_PTR swap = parent; + parent = children[called_nuc]; + children[called_nuc] = swap; + } read.state_inphase[solution_flow] = max(recent_state_inphase, 0.01f); read.state_total[solution_flow] = max(recent_state_total, 0.01f); - } + } + + if (flow_predictors_) { //if (flow_predictors_) + read.penalty_mismatch_flow.assign(num_flows, 0); + read.penalty_residual_flow.assign(num_flows, 0); + //vector flows_to_proc; + for (int solution_flow = 0; solution_flow < num_flows; ++solution_flow) { + int curr_base = flow_to_base[solution_flow]; + if (curr_base >= 0) { + assert (curr_base0) { + for (int i=0; i& flow_to_base, const bool flow_predictors_=false); + + int nuc_char_to_int(char nuc) {if (nuc=='A') return 0; if (nuc=='C') return 1; if (nuc=='G') return 2; if (nuc=='T') return 3; return -1;} protected: diff --git a/Analysis/BaseCaller/TreephaserVEC.cpp b/Analysis/BaseCaller/TreephaserVEC.cpp index c8f84c4f..a56e6154 100644 --- a/Analysis/BaseCaller/TreephaserVEC.cpp +++ b/Analysis/BaseCaller/TreephaserVEC.cpp @@ -1304,15 +1304,15 @@ void TreephaserSSE::WindowedNormalize(BasecallerRead& read, int num_steps) void TreephaserSSE::ComputeQVmetrics(BasecallerRead& read) { static const char nuc_int_to_char[5] = "ACGT"; - - read.state_inphase.assign(flow_order_.num_flows(), 1); - read.state_total.assign(flow_order_.num_flows(), 1); + int num_flows = flow_order_.num_flows(); + read.state_inphase.assign(num_flows, 1); + read.state_total.assign(num_flows, 1); if (read.sequence.empty()) return; - - read.penalty_mismatch.assign(read.sequence.size(), 0); - read.penalty_residual.assign(read.sequence.size(), 0); + int num_bases = read.sequence.size(); + read.penalty_mismatch.assign(num_bases, 0); + read.penalty_residual.assign(num_bases, 0); PathRec RESTRICT_PTR parent = sv_PathPtr[0]; PathRec RESTRICT_PTR children[4] = {sv_PathPtr[1], sv_PathPtr[2], sv_PathPtr[3], sv_PathPtr[4]}; @@ -1332,33 +1332,157 @@ void TreephaserSSE::ComputeQVmetrics(BasecallerRead& read) float recent_state_total = 1; // main loop for base calling - for (int solution_flow = 0, base = 0; solution_flow < flow_order_.num_flows(); ++solution_flow) { - for (; base < (int)read.sequence.size() and read.sequence[base] == flow_order_[solution_flow]; ++base) { + for (int solution_flow = 0, base = 0; solution_flow < num_flows; ++solution_flow) { + for (; basecalib_A[parent->flow] = (*As_).at(parent->flow).at(flow_order_.int_at(parent->flow)).at(parent->last_hp); + parent->calib_B[parent->flow] = (*Bs_).at(parent->flow).at(flow_order_.int_at(parent->flow)).at(parent->last_hp); + } + // compute child path flow states, predicted signal,negative and positive penalties + advanceState4(parent, num_flows); - float penalty[4] = { 0, 0, 0, 0 }; + float penalty[4] = { 0, 0, 0, 0 }; + int called_nuc = -1; + for(int nuc = 0; nuc < 4; ++nuc) { + PathRec RESTRICT_PTR child = children[nuc]; + if (nuc_int_to_char[nuc] == flow_order_[solution_flow]) + called_nuc = nuc; + child->flow = min(ad_Idx.A[nuc], flow_order_.num_flows()); + child->window_end = min(ad_End.A[nuc], flow_order_.num_flows()); + child->window_start = min(ad_Beg.A[nuc], child->window_end); - int called_nuc = -1; + // Apply easy termination rules + if (child->flow >= num_flows || parent->last_hp >= MAX_HPXLEN ) { + penalty[nuc] = 25; // Mark for deletion + continue; + } - if(recalibrate_predictions_) { - parent->calib_A[parent->flow] = (*As_).at(parent->flow).at(flow_order_.int_at(parent->flow)).at(parent->last_hp); - parent->calib_B[parent->flow] = (*Bs_).at(parent->flow).at(flow_order_.int_at(parent->flow)).at(parent->last_hp); + // pointer in the ad_Buf buffer pointing at the running sum of positive residuals at start of parent window +// char RESTRICT_PTR pn = ad_Buf+nuc*4+(AD_NRES_OFS-16)-parent->window_start*16; + + // sum of squared residuals for positive residuals for flows < child->flow + float penPar = pres_Buf[child->flow-parent->window_start].A[nuc];// *((float RESTRICT_PTR)(ad_Buf+nuc*4+(AD_PRES_OFS)+(child->flow-parent->window_start-1)*16)); + + // sum of squared residuals for negative residuals for flows < child->window_end + float penNeg = nres_Buf[child->window_end-parent->window_start].A[nuc];// *((float RESTRICT_PTR)(ad_Buf+nuc*4+AD_NRES_OFS+(child->window_end-parent->window_start-1)*16)); + + penalty[nuc] = penPar + penNeg; } - // compute child path flow states, predicted signal,negative and positive penalties - advanceState4(parent, flow_order_.num_flows()); + // find current incorporating base + assert(called_nuc > -1); + assert(children[called_nuc]->flow == solution_flow); + PathRec RESTRICT_PTR childToKeep = children[called_nuc]; + //copy +// char RESTRICT_PTR p = ad_Buf+ called_nuc*4 + AD_STATE_OFS; + + recent_state_total = 0; + for(int i = parent->window_start, j = 1, e = childToKeep->window_end; i < e; ++i, j ++) { + childToKeep->state[i] = state_Buf[j].A[called_nuc];// *((float*)(p+j*16)); + childToKeep->pred[i] = pred_Buf[j].A[called_nuc];// *((float*)(p+j*16+(AD_PRED_OFS-AD_STATE_OFS))); + recent_state_total += childToKeep->state[i]; + } + //sse implementation with aligned memory; no gain as the number of elements to be summed up is small +// recent_state_total = vecSumSSE(state_Buf, countStates); + + copySSE(childToKeep->pred, parent->pred, parent->window_start << 2); + + if (childToKeep->flow == parent->flow) + childToKeep->last_hp = parent->last_hp + 1; + else + childToKeep->last_hp = 1; + + recent_state_inphase = childToKeep->state[solution_flow]; + + // Get delta penalty to next best solution + read.penalty_mismatch[base] = -1; // min delta penalty to earlier base hypothesis + read.penalty_residual[base] = 0; + + if (solution_flow - parent->window_start > 0) + read.penalty_residual[base] = penalty[called_nuc] / (solution_flow - parent->window_start); + + for (int nuc = 0; nuc < 4; ++nuc) { + if (nuc == called_nuc) + continue; + float penalty_mismatch = penalty[called_nuc] - penalty[nuc]; + read.penalty_mismatch[base] = max(read.penalty_mismatch[base], penalty_mismatch); + } + + // Called state is the starting point for next base + PathRec RESTRICT_PTR swap = parent; + parent = children[called_nuc]; + children[called_nuc] = swap; + } + read.state_inphase[solution_flow] = max(recent_state_inphase, 0.01f); + read.state_total[solution_flow] = max(recent_state_total, 0.01f); + } + + if(recalibrate_predictions_) { + RecalibratePredictions(parent); + ResetRecalibrationStructures(num_flows_); + } + setZeroSSE(&read.prediction[0], num_flows_*sizeof(float)); + copySSE(&read.prediction[0], parent->pred, parent->window_end*sizeof(float)); +} + + +void TreephaserSSE::ComputeQVmetrics_flow(BasecallerRead& read, vector& flow_to_base, const bool flow_predictors_) +{ + static const char nuc_int_to_char[5] = "ACGT"; + int num_flows = flow_order_.num_flows(); + read.state_inphase.assign(num_flows, 1); + read.state_total.assign(num_flows, 1); + + if (read.sequence.empty()) + return; + int num_bases = read.sequence.size(); + read.penalty_mismatch.assign(num_bases, 0); + read.penalty_residual.assign(num_bases, 0); + if (flow_predictors_) { + read.penalty_mismatch_flow.assign(num_flows, 0); + read.penalty_residual_flow.assign(num_flows, 0); + } + + PathRec RESTRICT_PTR parent = sv_PathPtr[0]; + PathRec RESTRICT_PTR children[4] = {sv_PathPtr[1], sv_PathPtr[2], sv_PathPtr[3], sv_PathPtr[4]}; + parent->flow = 0; + parent->window_start = 0; + parent->window_end = 1; + parent->res = 0.0f; + parent->metr = 0.0f; + parent->flowMetr = 0.0f; + parent->dotCnt = 0; + parent->state[0] = 1.0f; + parent->sequence_length = 0; + parent->last_hp = 0; + parent->pred[0] = 0.0f; + + float recent_state_inphase = 1; + float recent_state_total = 1; + + // main loop for base calling + for (int solution_flow = 0, base = 0; solution_flow < num_flows; ++solution_flow) { + for (; basecalib_A[parent->flow] = (*As_).at(parent->flow).at(flow_order_.int_at(parent->flow)).at(parent->last_hp); + parent->calib_B[parent->flow] = (*Bs_).at(parent->flow).at(flow_order_.int_at(parent->flow)).at(parent->last_hp); + } + // compute child path flow states, predicted signal,negative and positive penalties + advanceState4(parent, num_flows); + + float penalty[4] = { 0, 0, 0, 0 }; + int called_nuc = -1; for(int nuc = 0; nuc < 4; ++nuc) { PathRec RESTRICT_PTR child = children[nuc]; - if (nuc_int_to_char[nuc] == flow_order_[solution_flow]) called_nuc = nuc; - child->flow = min(ad_Idx.A[nuc], flow_order_.num_flows()); child->window_end = min(ad_End.A[nuc], flow_order_.num_flows()); child->window_start = min(ad_Beg.A[nuc], child->window_end); // Apply easy termination rules - if (child->flow >= flow_order_.num_flows() || parent->last_hp >= MAX_HPXLEN ) { + if (child->flow >= num_flows || parent->last_hp >= MAX_HPXLEN ) { penalty[nuc] = 25; // Mark for deletion continue; } @@ -1420,10 +1544,35 @@ void TreephaserSSE::ComputeQVmetrics(BasecallerRead& read) parent = children[called_nuc]; children[called_nuc] = swap; } - read.state_inphase[solution_flow] = max(recent_state_inphase, 0.01f); read.state_total[solution_flow] = max(recent_state_total, 0.01f); - } + } + + if (flow_predictors_) { //if (flow_predictors_) + //vector flows_to_proc; + for (int solution_flow = 0; solution_flow < num_flows; ++solution_flow) { + int curr_base = flow_to_base[solution_flow]; + if (curr_base >= 0) { + // copy from what's stored in read.penalty_mismatch[base] + read.penalty_mismatch_flow[solution_flow] = read.penalty_mismatch[curr_base]; + read.penalty_residual_flow[solution_flow] = read.penalty_residual[curr_base]; + /* + int nFlows = flows_to_proc.size(); + if (nFlows>0) { + for (int i=0; i& flow_to_base, const bool flow_predictors_=false); + + int nuc_char_to_int(char nuc) {if (nuc=='A') return 0; if (nuc=='C') return 1; if (nuc=='G') return 2; if (nuc=='T') return 3; return -1;} protected: diff --git a/Analysis/BkgModel/Bookkeeping/BeadTracker.cpp b/Analysis/BkgModel/Bookkeeping/BeadTracker.cpp index c9db7d60..448094fb 100644 --- a/Analysis/BkgModel/Bookkeeping/BeadTracker.cpp +++ b/Analysis/BkgModel/Bookkeeping/BeadTracker.cpp @@ -1,7 +1,7 @@ /* Copyright (C) 2010 Ion Torrent Systems, Inc. All Rights Reserved */ #include #include "BeadTracker.h" -#include "mixed.h" +#include "ClonalFilter/mixed.h" #include "ImageLoader.h" #include "MathUtil.h" #include "LevMarState.h" diff --git a/Analysis/BkgModel/Bookkeeping/EmphasisVector.cpp b/Analysis/BkgModel/Bookkeeping/EmphasisVector.cpp index aded1b2a..b9f242fc 100644 --- a/Analysis/BkgModel/Bookkeeping/EmphasisVector.cpp +++ b/Analysis/BkgModel/Bookkeeping/EmphasisVector.cpp @@ -81,7 +81,8 @@ int RescaleVector ( float *vect, int npts ) } // just a gaussian -int GenerateBlankEmphasis ( float *vect, float *emp, int tsize, float t_center, const vector& frames_per_point, const vector& frameNumber ) +int GenerateBlankEmphasis ( float *vect, float *emp, int tsize, float t_center, + const vector& frames_per_point, const vector& frameNumber ) { int npts = tsize; @@ -97,8 +98,27 @@ int GenerateBlankEmphasis ( float *vect, float *emp, int tsize, float t_center, return ( npts ); } +// t-distribution heavier tailed but has natural scale span +int BlankDataWeight(float *vect, int tsize, + float t_center, const vector& frames_per_point, const vector& frameNumber, + float blank_span){ + int npts = tsize; + for (int i=0; i& frames_per_point, const vector& frameNumber, float width, float ampl ) +int GenerateStratifiedEmphasis ( float *vect, int vn, float *emp, int tsize, + float t_center, const vector& frames_per_point, const vector& frameNumber, + float width, float ampl ) { int npts = tsize; float na = emp[0]+vn*emp[1]; @@ -124,6 +144,37 @@ int GenerateStratifiedEmphasis ( float *vect, int vn, float *emp, int tsize, flo return ( npts ); } +// fix from offsets +int GenerateNmerDataWeights(float *vect, int tsize, int vn, + float t_center, const vector& frames_per_point, const vector& frameNumber, + DataWeightDefaults &data_weights + ){ + // we fall to zero here + float nmer_zero = t_center+data_weights.zero_span+vn*data_weights.nmer_span_increase; + // we fall to zero at this rate + float zero_slope= (data_weights.zero_span*(1.0f-data_weights.zero_fade_start)); + if (zero_slope<1.0f ) zero_slope =1.0f; // 1 frame drop minimum + zero_slope = -1.0f/zero_slope; // no divide by zero, no weird negatives + + float offset= -1.0f * zero_slope*nmer_zero; + int npts = tsize; + for (int i=0; i1.0f) tmp_weight = 1.0f; + + // now apply constraint for prefix + if (t_center-frameNumber[i]>=data_weights.prefix_start) tmp_weight=data_weights.prefix_value; + vect[i] = frames_per_point[i]; + vect[i] *= tmp_weight; + + } + RescaleVector(vect, npts); + return(npts); +} + // prepare to export the default math as a separate routine // so that Rcpp can wrap it and we see what we expect to see @@ -155,23 +206,39 @@ void EmphasisClass::BuildCurrentEmphasisTable ( float t_center, float amult ) local_frames_per_point = my_frames_per_point; else{ local_frames_per_point.assign(my_frames_per_point.size(),1); // no weighting by compression to get rid of edge artifacts - int cur_lowest = 1000; - // annoyingly, it looks like I need to keep the emphasis at the >start< for the clonal penalty - //@TODO: fix the clonal penalty in multiflow lev mar to be sensible instead of senseless - for (unsigned int i=0; i0.0f){ + EmphasisScale[vn]=GenerateNmerDataWeights(vect, npts, vn, + t_center, local_frames_per_point, my_frameNumber, + data_weights); + + } else { + EmphasisScale[vn] = BlankDataWeight(vect, npts, + t_center, local_frames_per_point, my_frameNumber, + data_weights.blank_span); + } +/* + int vm=vn+1; + if (vm>=numEv) vm=vn; + float *tect = EmphasisVectorByHomopolymer[vm] = &emphasis_vector_storage[npts*(vm)]; + GenerateIndividualEmphasis ( tect, vn, emp, npts, + t_center, local_frames_per_point, my_frameNumber, + amult,emphasis_width, emphasis_ampl ); // actual data + for(int vp=0; vp emphasis_vector_storage; // storage for emphasis vectors float** EmphasisVectorByHomopolymer; // array of pointers to different vectors std::vector EmphasisScale; // scaling factor for each vector - float emp[NUMEMPHASISPARAMETERS]; // parameters for emphasis vector generation std::vector nonZeroEmphasisFrames; // number of non zero frame values for each emphasis vector + float emp[NUMEMPHASISPARAMETERS]; // parameters for emphasis vector generation bool point_emphasis_by_compression; // avoid emphasis artifacts due to highly compressed points // keep timing parameters as well float emphasis_width; // parameters scaling the emphasis vector float emphasis_ampl; // parameters scaling the emphasis vector + + DataWeightDefaults data_weights; // real control + // timing parameters - warning, if time-compression changes these need to be updated std::vector my_frames_per_point; std::vector my_frameNumber; @@ -41,6 +46,8 @@ class EmphasisClass EmphasisClass(); ~EmphasisClass(); + void SetUpEmphasis(TimeAndEmphasisDefaults &data_control, TimeCompression &time_c); + private: void DetermineNonZeroEmphasisFrames(int hp); void AllocateScratch(); @@ -65,6 +72,7 @@ class EmphasisClass & my_frames_per_point & my_frameNumber & npts + & data_weights & point_emphasis_by_compression; if ( npts > 0 ) @@ -88,6 +96,7 @@ class EmphasisClass & my_frames_per_point & my_frameNumber & npts + & data_weights & point_emphasis_by_compression; // fprintf(stdout, "done EmphasisVector\n"); diff --git a/Analysis/BkgModel/Bookkeeping/RegionParamDefault.h b/Analysis/BkgModel/Bookkeeping/RegionParamDefault.h index 96603412..04c090f2 100644 --- a/Analysis/BkgModel/Bookkeeping/RegionParamDefault.h +++ b/Analysis/BkgModel/Bookkeeping/RegionParamDefault.h @@ -23,8 +23,8 @@ struct RegionParamDefault{ float tau_R_o_default; float tau_E_default; float min_tauB_default; - float max_tauB_default; + float tauB_smooth_range_default; float tshift_default; float krate_default[NUMNUC]; diff --git a/Analysis/BkgModel/Bookkeeping/TimeCompression.h b/Analysis/BkgModel/Bookkeeping/TimeCompression.h index 82520e8f..d73cd012 100644 --- a/Analysis/BkgModel/Bookkeeping/TimeCompression.h +++ b/Analysis/BkgModel/Bookkeeping/TimeCompression.h @@ -12,7 +12,7 @@ #include "Serialization.h" #include "IonErr.h" #include "VectorMacros.h" -#include "Image.h" +//#include "Image.h" #if defined( __SSE__ ) && !defined( __CUDACC__ ) #include diff --git a/Analysis/BkgModel/Bookkeeping/TimeControl.cpp b/Analysis/BkgModel/Bookkeeping/TimeControl.cpp index 59463021..b35b560c 100644 --- a/Analysis/BkgModel/Bookkeeping/TimeControl.cpp +++ b/Analysis/BkgModel/Bookkeeping/TimeControl.cpp @@ -9,21 +9,50 @@ TimeAndEmphasisDefaults::TimeAndEmphasisDefaults() time_left_avg = 5; time_start_detail = -5; time_stop_detail = 16; + +} + +EmphasisDefaults::EmphasisDefaults(){ point_emphasis_by_compression = true; memcpy(emp,_emp,sizeof(float[NUMEMPHASISPARAMETERS])); emphasis_ampl_default = 7.25; emphasis_width_default = 2.89; + +} + +DataWeightDefaults::DataWeightDefaults(){ + blank_span = 17.0f; + zero_span = 17.0f; + nmer_span_increase = 1.0f; + zero_fade_start = 0.5f; + prefix_start = 3; + prefix_value = 0.15f; + use_data_weight = false; + } -void TimeAndEmphasisDefaults::DumpPoorlyStructuredText(){ +void EmphasisDefaults::DumpPoorlyStructuredText(){ printf ( "emphasis: %f\t%f\t%f\t%f\t%f\t%f\t%f\t%f\n",emp[0],emp[1],emp[2],emp[3],emp[4],emp[5],emp[6],emp[7] ); printf ( "emp_amplitude: \t%f\n",emphasis_ampl_default ); printf ( "emp_width: \t%f\n",emphasis_width_default ); } -void TimeAndEmphasisDefaults::FromJson(Json::Value &gopt_params){ +void DataWeightDefaults::FromJson(Json::Value &gopt_params){ + if (!gopt_params["DataWeight"].isNull()){ + use_data_weight=true; + const Json::Value data_weight = gopt_params["DataWeight"]; + blank_span = data_weight["blank_span"].asFloat(); + zero_span = data_weight["zero_span"].asFloat(); + nmer_span_increase = data_weight["nmer_span_increase"].asFloat(); + zero_fade_start = data_weight["zero_fade_start"].asFloat(); + prefix_start = data_weight["prefix_start"].asFloat(); + prefix_value = data_weight["prefix_value"].asFloat(); + } +} + +void EmphasisDefaults::FromJson(Json::Value &gopt_params){ const Json::Value emphasis = gopt_params["emphasis"]; for ( int index = 0; index < (int) emphasis.size(); ++index ) emp[index] = emphasis[index].asFloat(); @@ -31,3 +60,8 @@ void TimeAndEmphasisDefaults::FromJson(Json::Value &gopt_params){ emphasis_ampl_default = gopt_params["emp_amplitude"].asFloat(); emphasis_width_default = gopt_params["emp_width"].asFloat(); } + +void TimeAndEmphasisDefaults::FromJson(Json::Value &gopt_params){ + emphasis_params.FromJson(gopt_params); + data_weights.FromJson(gopt_params); +} diff --git a/Analysis/BkgModel/Bookkeeping/TimeControl.h b/Analysis/BkgModel/Bookkeeping/TimeControl.h index d69b00ff..c8f8a9e2 100644 --- a/Analysis/BkgModel/Bookkeeping/TimeControl.h +++ b/Analysis/BkgModel/Bookkeeping/TimeControl.h @@ -12,13 +12,38 @@ #include "BkgMagicDefines.h" #include "Serialization.h" +struct DataWeightDefaults{ -struct TimeAndEmphasisDefaults{ - float nuc_flow_frame_width; - int time_left_avg; - int time_start_detail; - int time_stop_detail; + // revised emphasis parameterization for clarity + // linear approximation to weighting + bool use_data_weight; + float blank_span; // generic emphasis span (17 frames) + float zero_span; // span for a zero-mer emphasis (13 frames) + float nmer_span_increase; // frames per additiona n-mer over a zeromer (1.0 frames) + float zero_fade_start; // fraction of zeromer span to fade-to-zero weight over (0.5) + float prefix_start; // offset for prefix = 3 (-3 frames) + float prefix_value; // fraction of maximum value = 0.15 + + DataWeightDefaults(); + void FromJson(Json::Value &gopt_params); +private: + friend class boost::serialization::access; + template + void serialize(Archive& ar, const unsigned int version) + { + ar + & blank_span + & zero_span + & nmer_span_increase + & zero_fade_start + & prefix_start + & prefix_value + & use_data_weight; + } + +}; +struct EmphasisDefaults{ //@TODO: why are these not simply part of "EmphasisVector.h"? float emphasis_ampl_default; float emphasis_width_default; @@ -26,26 +51,51 @@ struct TimeAndEmphasisDefaults{ float emp[NUMEMPHASISPARAMETERS]; // parameters for emphasis vector generation bool point_emphasis_by_compression; - TimeAndEmphasisDefaults(); + EmphasisDefaults(); void DumpPoorlyStructuredText(); void FromJson(Json::Value &gopt_params); +private: + friend class boost::serialization::access; + template + void serialize(Archive& ar, const unsigned int version) + { + ar + & emphasis_ampl_default + & emphasis_width_default + & numEv + & emp; + } +}; + + +struct TimeAndEmphasisDefaults{ + float nuc_flow_frame_width; + int time_left_avg; + int time_start_detail; + int time_stop_detail; + + + DataWeightDefaults data_weights; + EmphasisDefaults emphasis_params; + + TimeAndEmphasisDefaults(); + + void FromJson(Json::Value &gopt_params); private: friend class boost::serialization::access; template void serialize(Archive& ar, const unsigned int version) { ar + & data_weights + & nuc_flow_frame_width & time_left_avg & time_start_detail & time_stop_detail - & emphasis_ampl_default - & emphasis_width_default - & numEv - & point_emphasis_by_compression - & emp; + &emphasis_params; } }; diff --git a/Analysis/BkgModel/CUDA/GpuMultiFlowFitControl.cpp b/Analysis/BkgModel/CUDA/GpuMultiFlowFitControl.cpp index debc15cb..2b14eac1 100644 --- a/Analysis/BkgModel/CUDA/GpuMultiFlowFitControl.cpp +++ b/Analysis/BkgModel/CUDA/GpuMultiFlowFitControl.cpp @@ -41,10 +41,9 @@ GpuMultiFlowFitMatrixConfig* GpuMultiFlowFitControl::createConfig( { // Build the configuration. GpuMultiFlowFitMatrixConfig* config = - new GpuMultiFlowFitMatrixConfig(levMarSparseMatrices->GetFitDescriptorByName(fitName.c_str()), + new GpuMultiFlowFitMatrixConfig(const_cast(levMarSparseMatrices)->GetFitDescriptorByName(fitName.c_str()), BkgFitStructures::Steps, BkgFitStructures::NumSteps, _activeFlowKey, _activeFlowMax); - // Accumulate maximum values. DetermineMaxSteps(config->GetNumSteps()); DetermineMaxParams(config->GetNumParamsToFit()); @@ -54,7 +53,6 @@ GpuMultiFlowFitMatrixConfig* GpuMultiFlowFitControl::createConfig( return config; } - void GpuMultiFlowFitControl::DetermineMaxSteps(int steps) { if (_maxSteps < steps) diff --git a/Analysis/BkgModel/CUDA/GpuMultiFlowFitMatrixConfig.cpp b/Analysis/BkgModel/CUDA/GpuMultiFlowFitMatrixConfig.cpp index 368724a8..63104627 100644 --- a/Analysis/BkgModel/CUDA/GpuMultiFlowFitMatrixConfig.cpp +++ b/Analysis/BkgModel/CUDA/GpuMultiFlowFitMatrixConfig.cpp @@ -3,27 +3,27 @@ #include "CudaDefines.h" #include "GpuMultiFlowFitMatrixConfig.h" -GpuMultiFlowFitMatrixConfig::GpuMultiFlowFitMatrixConfig(fit_descriptor* fd, CpuStep* Steps, int maxSteps, int flow_key, int flow_block_size) +GpuMultiFlowFitMatrixConfig::GpuMultiFlowFitMatrixConfig(const std::vector& fds, CpuStep* Steps, int maxSteps, int flow_key, int flow_block_size) { // num of partial derivative steps to compute for this fit descriptor - _numSteps = BkgFitStructures::GetNumParDerivStepsForFitDescriptor(fd); + _numSteps = BkgFitStructures::GetNumParDerivStepsForFitDescriptor(fds); // number of actual partial derivative steps to compute _numSteps = _numSteps + 2; // Need to calculate FVAL and YERR always // calculate num of params to fit based on param sensitivity classification - _numParamsToFit = BkgFitStructures::GetNumParamsToFitForDescriptor(fd, flow_key, flow_block_size); + _numParamsToFit = BkgFitStructures::GetNumParamsToFitForDescriptor(fds, flow_key, flow_block_size); // collect partial derivative steps from Steps structure in // BkgFitStructures.cpp for this fit - CreatePartialDerivStepsVector(fd, Steps, maxSteps); + CreatePartialDerivStepsVector(fds, Steps, maxSteps); _paramIdxMap = new unsigned int[_numParamsToFit]; _affectedFlowsForParamsBitMap = new unsigned int[_numParamsToFit]; _paramToStepMap = new unsigned int[_numParamsToFit]; _jtjMatrixBitMap = new unsigned int[_numParamsToFit*_numParamsToFit]; - CreateAffectedFlowsVector(fd, flow_key, flow_block_size); + CreateAffectedFlowsVector(fds, flow_key, flow_block_size); CreateBitMapForJTJMatrixComputation(); } @@ -36,7 +36,7 @@ GpuMultiFlowFitMatrixConfig::~GpuMultiFlowFitMatrixConfig() delete [] _paramToStepMap; } -void GpuMultiFlowFitMatrixConfig::CreatePartialDerivStepsVector(fit_descriptor* fd, CpuStep* Steps, int maxSteps) +void GpuMultiFlowFitMatrixConfig::CreatePartialDerivStepsVector(const std::vector& fds, CpuStep* Steps, int maxSteps) { _partialDerivSteps = new CpuStep[_numSteps]; @@ -46,11 +46,11 @@ void GpuMultiFlowFitMatrixConfig::CreatePartialDerivStepsVector(fit_descriptor* if (Steps[maxSteps - 1].PartialDerivMask == YERR) _partialDerivSteps[_numSteps - 1] = Steps[maxSteps - 1]; - for (int i=1; fd[i-1].comp != TBL_END; ++i) + for (int i=1; fds[i-1].comp != TBL_END; ++i) { for (int j=0; j& fds, int flow_key, int flow_block_size ) @@ -70,13 +70,13 @@ void GpuMultiFlowFitMatrixConfig::CreateAffectedFlowsVector( unsigned int paramIdx = 0; unsigned int actualStep = 1; - for (int i=0; fd[i].comp != TBL_END; ++i) + for (int i=0; fds[i].comp != TBL_END; ++i) { // Calculate a base index for reaching into the BeadParams / reg_params structure. - unsigned int baseIndex = fd[i].bead_params_func ? ( dummyBead.*( fd[i].bead_params_func ))() - reinterpret_cast< float * >( & dummyBead ) - : ( dummyReg .*( fd[i].reg_params_func ))() - reinterpret_cast< float * >( & dummyReg ); + unsigned int baseIndex = fds[i].bead_params_func ? ( dummyBead.*( fds[i].bead_params_func ))() - reinterpret_cast< float * >( & dummyBead ) + : ( dummyReg .*( fds[i].reg_params_func ))() - reinterpret_cast< float * >( & dummyReg ); - switch(fd[i].ptype) + switch(fds[i].ptype) { case ParamTypeAllFlow: _paramIdxMap[paramIdx] = baseIndex; diff --git a/Analysis/BkgModel/CUDA/GpuMultiFlowFitMatrixConfig.h b/Analysis/BkgModel/CUDA/GpuMultiFlowFitMatrixConfig.h index 4272eaf5..571f15c6 100644 --- a/Analysis/BkgModel/CUDA/GpuMultiFlowFitMatrixConfig.h +++ b/Analysis/BkgModel/CUDA/GpuMultiFlowFitMatrixConfig.h @@ -8,7 +8,7 @@ class GpuMultiFlowFitMatrixConfig { public: - GpuMultiFlowFitMatrixConfig(fit_descriptor*, CpuStep*, int maxSteps, int flow_key, int flow_block_size); + GpuMultiFlowFitMatrixConfig(const std::vector& fds, CpuStep*, int maxSteps, int flow_key, int flow_block_size); ~GpuMultiFlowFitMatrixConfig(); int GetNumSteps() { return _numSteps; } @@ -18,8 +18,8 @@ class GpuMultiFlowFitMatrixConfig CpuStep* GetPartialDerivSteps() { return _partialDerivSteps; } private: - void CreatePartialDerivStepsVector(fit_descriptor*, CpuStep*, int); - void CreateAffectedFlowsVector(fit_descriptor*, int flow_key, int flow_block_size); + void CreatePartialDerivStepsVector(const std::vector& fds, CpuStep*, int); + void CreateAffectedFlowsVector(const std::vector& fds, int flow_key, int flow_block_size); void CreateBitMapForJTJMatrixComputation(); private: diff --git a/Analysis/BkgModel/CUDA/HostDataWrapper/ClonalFilterWrapper.cpp b/Analysis/BkgModel/CUDA/HostDataWrapper/ClonalFilterWrapper.cpp index e77496af..47da144d 100644 --- a/Analysis/BkgModel/CUDA/HostDataWrapper/ClonalFilterWrapper.cpp +++ b/Analysis/BkgModel/CUDA/HostDataWrapper/ClonalFilterWrapper.cpp @@ -7,7 +7,7 @@ */ #include "ClonalFilterWrapper.h" -#include "mixed.h" +#include "ClonalFilter/mixed.h" #include "hdf5.h" #define MIXED_PPF_CUTOFF 0.84f diff --git a/Analysis/BkgModel/CUDA/HostDataWrapper/ClonalFilterWrapper.h b/Analysis/BkgModel/CUDA/HostDataWrapper/ClonalFilterWrapper.h index 61855766..3f9e056b 100644 --- a/Analysis/BkgModel/CUDA/HostDataWrapper/ClonalFilterWrapper.h +++ b/Analysis/BkgModel/CUDA/HostDataWrapper/ClonalFilterWrapper.h @@ -11,7 +11,7 @@ #include #include -#include "polyclonal_filter.h" +#include "ClonalFilter/polyclonal_filter.h" #include "LayoutTranslator.h" diff --git a/Analysis/BkgModel/Fitters/Complex/BkgFitMatDat.h b/Analysis/BkgModel/Fitters/Complex/BkgFitMatDat.h index 8dbdd807..0eb00a72 100644 --- a/Analysis/BkgModel/Fitters/Complex/BkgFitMatDat.h +++ b/Analysis/BkgModel/Fitters/Complex/BkgFitMatDat.h @@ -4,7 +4,7 @@ #include -class BkgFitMatDat { +class BkgFitMatDat { public: arma::Mat *jtj; arma::Col *rhs; diff --git a/Analysis/BkgModel/Fitters/Complex/BkgFitMatrixPacker.cpp b/Analysis/BkgModel/Fitters/Complex/BkgFitMatrixPacker.cpp index 5d7a3fc6..48761cbe 100644 --- a/Analysis/BkgModel/Fitters/Complex/BkgFitMatrixPacker.cpp +++ b/Analysis/BkgModel/Fitters/Complex/BkgFitMatrixPacker.cpp @@ -49,6 +49,8 @@ BkgFitMatrixPacker:: BkgFitMatrixPacker (int imgLen,fit_instructions &fi,Partial nOutputs = fi.output_len; outputList = fi.output; + for (int i=0; idelta->at (i))) + if (std::isnan (data->delta->at (i))) { delta_ok = false; break; diff --git a/Analysis/BkgModel/Fitters/Complex/BkgFitMatrixPacker.h b/Analysis/BkgModel/Fitters/Complex/BkgFitMatrixPacker.h index d54bf92b..d4349569 100644 --- a/Analysis/BkgModel/Fitters/Complex/BkgFitMatrixPacker.h +++ b/Analysis/BkgModel/Fitters/Complex/BkgFitMatrixPacker.h @@ -9,6 +9,7 @@ #include "BkgMagicDefines.h" #include "BeadParams.h" #include "RegionParams.h" +#include class BkgFitMatDat; @@ -81,7 +82,7 @@ class mat_assy_input_line { mat_row = mat_col = 0; matId = JTJ_MAT; } - ~mat_assy_input_line() { delete [] affected_flows; } + ~mat_assy_input_line() { if (affected_flows) delete [] affected_flows; } void realloc( int size ) { delete [] affected_flows; affected_flows = new int[size]; } PartialDerivComponent comp1; @@ -93,6 +94,7 @@ class mat_assy_input_line { AssyMatID matId; int mat_row; int mat_col; + std::string derivName; }; @@ -115,10 +117,12 @@ struct mat_assembly_instruction { // structure that holds the output mapping of the solution matrix (the delta vector) // to the params structure struct delta_mat_output_line { + delta_mat_output_line() : delta_ndx(0), bead_params_func(NULL), reg_params_func(NULL), array_index(0) {} int delta_ndx; float * ( BeadParams::* bead_params_func )(); float * ( reg_params::* reg_params_func )(); int array_index; + std::string name; }; // collection of all input and output instruction information for a single fit configuration @@ -127,6 +131,13 @@ struct fit_instructions { int input_len; struct delta_mat_output_line *output; int output_len; + + fit_instructions() { + input = NULL; + input_len = 0; + output = NULL; + output_len = 0; + } }; typedef enum { @@ -152,6 +163,7 @@ class BkgFitMatrixPacker double *GetDestMatrixPtr(AssyMatID mat_id,int row,int col); + std::vector compNames; private: @@ -175,12 +187,12 @@ class BkgFitMatrixPacker public: fit_instructions& my_fit_instructions; - delta_mat_output_line* getOuputList() { return outputList; } - mat_assembly_instruction* getInstList() { return instList; } + delta_mat_output_line* getOuputList() const { return outputList; } + mat_assembly_instruction* getInstList() const { return instList; } void BuildMatrix(bool accum); - int getNumInstr() { return nInstr; } - int getNumOutputs() { return nOutputs; } - int getNumException() { return numException; } + int getNumInstr() const { return nInstr; } + int getNumOutputs() const { return nOutputs; } + int getNumException() const { return numException; } void resetNumException() { numException = 0; } void SetDataRhs(float, int); void SetDataJtj(float, int, int); diff --git a/Analysis/BkgModel/Fitters/Complex/BkgFitOptim.cpp b/Analysis/BkgModel/Fitters/Complex/BkgFitOptim.cpp index a3513ddb..52506801 100644 --- a/Analysis/BkgModel/Fitters/Complex/BkgFitOptim.cpp +++ b/Analysis/BkgModel/Fitters/Complex/BkgFitOptim.cpp @@ -3,12 +3,35 @@ #include "BkgFitOptim.h" #include "BkgFitStructures.h" +using namespace std; +#define DVAL(X) {X,#X} +std::map PartialDerivNames = { + DVAL(TBL_END),DVAL(TBL_END),DVAL(DFDP),DVAL(DFDR), + DVAL(DFDPDM),DVAL(DFDGAIN),DVAL(DFDA),DVAL(DFDDKR), + DVAL(DFDSIGMA),DVAL(DFDTSH),DVAL(DFDERR),DVAL(DFDT0), + DVAL(DFDTAUMR),DVAL(DFDTAUOR),DVAL(DFDRDR),DVAL(DFDKRATE), + DVAL(DFDSENS),DVAL(DFDD),DVAL(DFDPDR),DVAL(DFDMR),DVAL(DFDKMAX), + DVAL(DFDT0DLY),DVAL(DFDSMULT),DVAL(DFDTAUE),DVAL(YERR),DVAL(FVAL) + }; -//@TODO BAD CODE STYLE: function in header -void InitializeLevMarFitter(mat_table_build_instr *btbl,fit_instructions *instr, int flow_block_size) +std::string ComponentName( PartialDerivComponent comp ) +{ + std::string readable_name; + try { + readable_name = PartialDerivNames[comp]; + if(readable_name.substr(0,3)=="DFD") + readable_name=readable_name.substr(3); //cleanup the readable name by removing redundant part + } catch (const std::out_of_range&) { + readable_name = "UNKNOWN"; + } + return readable_name; +} + + +void InitializeLevMarFitter(const mat_table_build_instr *btbl,fit_instructions *instr, int flow_block_size) { int np; @@ -48,9 +71,6 @@ void InitializeLevMarFitter(mat_table_build_instr *btbl,fit_instructions *instr, // the output matrix gets one row for each indepdendent parameter struct delta_mat_output_line *ols = new struct delta_mat_output_line[np]; - // it's a good idea to clear these at the start - memset(ols,0,sizeof(struct delta_mat_output_line[np])); - // now build the input and output lines input_cnt = 0; for (int i=0;ioutput_len = np; } + + + void DumpBuildInstructionTable(mat_table_build_instr *tbl, int flow_block_size) { for (int i=0;true;i++) { std::string pcomp; - switch (tbl[i].comp) - { - case TBL_END: - pcomp="TBL_END "; - break; - case DFDR: - pcomp="DFDR "; - break; - case DFDA: - pcomp="DFDA "; - break; - case DFDT0: - pcomp="DFDT0 "; - break; - case DFDP: - pcomp="DFDP "; - break; - case DFDTSH: - pcomp="DFDTSH "; - break; - case DFDSIGMA: - pcomp="DFDSIGMA "; - break; - case DFDKRATE: - pcomp="DFDKRATE "; - break; - case DFDD: - pcomp="DFDD "; - break; - case DFDRDR: - pcomp="DFDRDR "; - break; - case DFDGAIN: - pcomp="DFDGAIN "; - break; - case YERR: - pcomp="YERR "; - break; - default: - pcomp = "UNKNOWN"; - } + pcomp = ComponentName( tbl[i].comp ); printf("%s % 4d [",pcomp.c_str(), tbl[i].array_index ); for (int j=0;j < flow_block_size-1;j++) @@ -170,14 +154,14 @@ void master_fit_type_entry::CreateBuildInstructions(const int *my_nuc, int flow_ { // if there is a high-level fit descriptor, create a set of build instructions // from the high-level descriptor - if (fd != NULL) + if (fds.size()>0) { int row_cnt = 0; // first figure out how many entries the build instruction table will need - for (int i=0;fd[i].comp != TBL_END;i++) + for (int i=0;fds[i].comp != TBL_END;i++) { - switch (fd[i].ptype) + switch (fds[i].ptype) { case ParamTypeAFlows: case ParamTypeCFlows: @@ -222,28 +206,30 @@ void master_fit_type_entry::CreateBuildInstructions(const int *my_nuc, int flow_ row_cnt = 0; // now create the rows - for (int i=0;fd[i].comp != TBL_END;i++) + for (int i=0;fds[i].comp != TBL_END;i++) { - switch (fd[i].ptype) + switch (fds[i].ptype) { case ParamTypeAllFlow: - mb[row_cnt].comp = fd[i].comp; - mb[row_cnt].bead_params_func = fd[i].bead_params_func; - mb[row_cnt].reg_params_func = fd[i].reg_params_func; + mb[row_cnt].comp = fds[i].comp; + mb[row_cnt].bead_params_func = fds[i].bead_params_func; + mb[row_cnt].reg_params_func = fds[i].reg_params_func; for (int j=0;j < flow_block_size;j++) mb[row_cnt].SetAffectedFlow(j, 1); + mb[row_cnt].name = ComponentName(fds[i].comp); row_cnt++; break; case ParamTypeNotKey: // create an independent paramter per flow except for key flows for (int row=flow_key;row < flow_block_size;row++) { - mb[row_cnt].comp = fd[i].comp; + mb[row_cnt].comp = fds[i].comp; // individual parameters for each flow are assumed to be consecutive in the // BeadParams structure - mb[row_cnt].bead_params_func = fd[i].bead_params_func; - mb[row_cnt].reg_params_func = fd[i].reg_params_func; + mb[row_cnt].bead_params_func = fds[i].bead_params_func; + mb[row_cnt].reg_params_func = fds[i].reg_params_func; mb[row_cnt].array_index = row - flow_key; + mb[row_cnt].name = ComponentName(fds[i].comp)+"_f"+std::to_string(row); // indicate which flow this specific parameter affects mb[row_cnt].SetAffectedFlow(row, 1); @@ -254,13 +240,14 @@ void master_fit_type_entry::CreateBuildInstructions(const int *my_nuc, int flow_ // create an independent paramter per flow except for the first flow for (int row=1;row < flow_block_size;row++) { - mb[row_cnt].comp = fd[i].comp; + mb[row_cnt].comp = fds[i].comp; // individual parameters for each flow are assumed to be consecutive in the // BeadParams structure - mb[row_cnt].bead_params_func = fd[i].bead_params_func; - mb[row_cnt].reg_params_func = fd[i].reg_params_func; + mb[row_cnt].bead_params_func = fds[i].bead_params_func; + mb[row_cnt].reg_params_func = fds[i].reg_params_func; mb[row_cnt].array_index = row - 1; + mb[row_cnt].name = ComponentName(fds[i].comp)+"_f"+std::to_string(row); // indicate which flow this specific parameter affects mb[row_cnt].SetAffectedFlow(row, 1); @@ -271,13 +258,14 @@ void master_fit_type_entry::CreateBuildInstructions(const int *my_nuc, int flow_ // create an independent paramter per flow for (int row=0;row < flow_block_size;row++) { - mb[row_cnt].comp = fd[i].comp; + mb[row_cnt].comp = fds[i].comp; // individual parameters for each flow are assumed to be consecutive in the // bead_params structure - mb[row_cnt].bead_params_func = fd[i].bead_params_func; - mb[row_cnt].reg_params_func = fd[i].reg_params_func; + mb[row_cnt].bead_params_func = fds[i].bead_params_func; + mb[row_cnt].reg_params_func = fds[i].reg_params_func; mb[row_cnt].array_index = row; + mb[row_cnt].name = ComponentName(fds[i].comp)+"_f"+std::to_string(row); // indicate which flow this specific parameter affects mb[row_cnt].SetAffectedFlow(row, 1); @@ -288,13 +276,14 @@ void master_fit_type_entry::CreateBuildInstructions(const int *my_nuc, int flow_ // create an independent parameter per nucleotide for (int nuc=0;nuc < NUMNUC;nuc++) { - mb[row_cnt].comp = fd[i].comp; + mb[row_cnt].comp = fds[i].comp; // individual parameters for each nucleotide are assumed to be consecutive in the // BeadParams structure - mb[row_cnt].bead_params_func = fd[i].bead_params_func; - mb[row_cnt].reg_params_func = fd[i].reg_params_func; + mb[row_cnt].bead_params_func = fds[i].bead_params_func; + mb[row_cnt].reg_params_func = fds[i].reg_params_func; mb[row_cnt].array_index = nuc; + mb[row_cnt].name = ComponentName(fds[i].comp)+"_n"+std::to_string(nuc); // indicate which flows this specific parameter affects for (int j=0;j < flow_block_size;j++) @@ -310,11 +299,12 @@ void master_fit_type_entry::CreateBuildInstructions(const int *my_nuc, int flow_ break; case ParamTypeAFlows: // create one parameter for all flows of a single nucleotide (A) - mb[row_cnt].comp = fd[i].comp; + mb[row_cnt].comp = fds[i].comp; // the table entry should point to the specific parameter for this nucleotide - mb[row_cnt].bead_params_func = fd[i].bead_params_func; - mb[row_cnt].reg_params_func = fd[i].reg_params_func; + mb[row_cnt].bead_params_func = fds[i].bead_params_func; + mb[row_cnt].reg_params_func = fds[i].reg_params_func; + mb[row_cnt].name = ComponentName(fds[i].comp); // indicate which flows this specific parameter affects for (int j=0;j < flow_block_size;j++) @@ -329,11 +319,12 @@ void master_fit_type_entry::CreateBuildInstructions(const int *my_nuc, int flow_ break; case ParamTypeCFlows: // create one parameter for all flows of a single nucleotide (C) - mb[row_cnt].comp = fd[i].comp; + mb[row_cnt].comp = fds[i].comp; // the table entry should point to the specific parameter for this nucleotide - mb[row_cnt].bead_params_func = fd[i].bead_params_func; - mb[row_cnt].reg_params_func = fd[i].reg_params_func; + mb[row_cnt].bead_params_func = fds[i].bead_params_func; + mb[row_cnt].reg_params_func = fds[i].reg_params_func; + mb[row_cnt].name = ComponentName(fds[i].comp); // indicate which flows this specific parameter affects for (int j=0;j < flow_block_size;j++) @@ -348,11 +339,12 @@ void master_fit_type_entry::CreateBuildInstructions(const int *my_nuc, int flow_ break; case ParamTypeGFlows: // create one parameter for all flows of a single nucleotide (G) - mb[row_cnt].comp = fd[i].comp; + mb[row_cnt].comp = fds[i].comp; // the table entry should point to the specific parameter for this nucleotide - mb[row_cnt].bead_params_func = fd[i].bead_params_func; - mb[row_cnt].reg_params_func = fd[i].reg_params_func; + mb[row_cnt].bead_params_func = fds[i].bead_params_func; + mb[row_cnt].reg_params_func = fds[i].reg_params_func; + mb[row_cnt].name = ComponentName(fds[i].comp)+"_G"; // indicate which flows this specific parameter affects for (int j=0;j < flow_block_size;j++) @@ -379,60 +371,114 @@ void master_fit_type_entry::CreateBuildInstructions(const int *my_nuc, int flow_ InitializeLevMarFitter(mb,& (fi), flow_block_size); } - -master_fit_type_entry master_fit_type_table::base_bkg_model_fit_type[] = +void master_fit_type_table::set_base_bkg_model_fit_type() { -//{"name", &fit_descriptor, NULL, {NULL,0,NULL,0}}, - // individual well fits - {"FitWellAmpl", BkgFitStructures::fit_well_ampl_descriptor, NULL, {NULL,0,NULL,0}}, - {"FitWellAmplBuffering", BkgFitStructures::fit_well_ampl_buffering_descriptor, NULL, {NULL,0,NULL,0}}, - {"FitWellPostKey", BkgFitStructures::fit_well_post_key_descriptor, NULL, {NULL,0,NULL,0}}, - {"FitWellAll", BkgFitStructures::fit_well_all_descriptor, NULL, {NULL,0,NULL,0}}, - {"FitWellPostKeyNoDmult", BkgFitStructures::fit_well_post_key_descriptor_nodmult, NULL, {NULL,0,NULL,0}}, - - // region-wide fits - {"FitRegionTmidnucPlus", BkgFitStructures::fit_region_tmidnuc_plus_descriptor, NULL, {NULL,0,NULL,0}}, - {"FitRegionInit2", BkgFitStructures::fit_region_init2_descriptor, NULL, {NULL,0,NULL,0}}, - {"FitRegionInit2TauE", BkgFitStructures::fit_region_init2_taue_descriptor, NULL, {NULL,0,NULL,0}}, - {"FitRegionInit2TauENoRDR", BkgFitStructures::fit_region_init2_taue_NoRDR_descriptor, NULL, {NULL,0,NULL,0}}, - {"FitRegionFull", BkgFitStructures::fit_region_full_descriptor, NULL, {NULL,0,NULL,0}}, - {"FitRegionFullTauE", BkgFitStructures::fit_region_full_taue_descriptor, NULL, {NULL,0,NULL,0}}, - {"FitRegionFullTauENoRDR", BkgFitStructures::fit_region_full_taue_NoRDR_descriptor, NULL, {NULL,0,NULL,0}}, - {"FitRegionInit2NoRDR", BkgFitStructures::fit_region_init2_noRatioDrift_descriptor, NULL, {NULL,0,NULL,0}}, - {"FitRegionFullNoRDR", BkgFitStructures::fit_region_full_noRatioDrift_descriptor, NULL, {NULL,0,NULL,0}}, - {"FitRegionTimeVarying", BkgFitStructures::fit_region_time_varying_descriptor, NULL, {NULL,0,NULL,0}}, - {"FitRegionDarkness", BkgFitStructures::fit_region_darkness_descriptor, NULL, {NULL,0,NULL,0}}, - - //region-wide fits without diffusion - {"FitRegionInit2TauENoD", BkgFitStructures::fit_region_init2_taue_NoD_descriptor, NULL, {NULL,0,NULL,0}}, - {"FitRegionInit2TauENoRDRNoD",BkgFitStructures::fit_region_init2_taue_NoRDR_NoD_descriptor, NULL, {NULL,0,NULL,0}}, - {"FitRegionFullTauENoD", BkgFitStructures::fit_region_full_taue_NoD_descriptor, NULL, {NULL,0,NULL,0}}, - {"FitRegionFullTauENoRDRNoD", BkgFitStructures::fit_region_full_taue_NoRDR_NoD_descriptor, NULL, {NULL,0,NULL,0}}, - - { NULL, NULL, NULL, {NULL,0,NULL,0} }, // end of table -}; - - -fit_instructions *master_fit_type_table::GetFitInstructionsByName(const char *name) const + // set fit_type_hash_table + fit_type_hash_table = { // nested list-initialization + {"FitWellAmpl", {"wellAmpl","TableEnd"}}, + {"FitWellAmplBuffering", {"wellR","wellAmpl","TableEnd"}}, + {"FitWellPostKey", {"wellR","wellCopies","wellDmult","wellAmplPostKey","TableEnd"}}, + {"FitWellAll", {"wellR","wellCopies","wellDmult","wellAmpl","wellKmult","TableEnd"}}, + {"FitWellPostKeyNoDmult", {"wellR","wellCopies","wellAmplPostKey","TableEnd"}}, + // region-wide fits + {"FitRegionTmidnucPlus", {"R","Copies","Ampl","TMidNuc","TableEnd"}}, + {"FitRegionInit2", {"R","Copies","Ampl","TShift","TMidNuc","Sigma","Krate","D","NucModifyRatio","TauRM","TauRO","RatioDrift","CopyDrift","TableEnd"}}, + {"FitRegionInit2TauE", {"R","Copies","Ampl","TShift","TMidNuc","Sigma","Krate","D","NucModifyRatio","TauE","RatioDrift","CopyDrift","TableEnd"}}, + {"FitRegionInit2TauENoRDR",{"R","Copies","Ampl","TShift","TMidNuc","Sigma","Krate","D","NucModifyRatio","TauE","CopyDrift","TableEnd"}}, + {"FitRegionFull", {"R","Copies","Ampl","TShift","TMidNuc","Sigma","Krate","D","NucModifyRatio","TauRM","TauRO","RatioDrift","CopyDrift","TableEnd"}}, + {"FitRegionFullTauE", {"R","Copies","Ampl","TShift","TMidNuc","Sigma","Krate","D","NucModifyRatio","TauE","RatioDrift","CopyDrift","TableEnd"}}, + {"FitRegionFullTauENoRDR", {"R","Copies","Ampl","TShift","TMidNuc","Sigma","Krate","D","NucModifyRatio","TauE","CopyDrift","TableEnd"}}, + {"FitRegionInit2NoRDR", {"R","Copies","Ampl","TShift","TMidNuc","Sigma","TMidNucDelay","SigmaMult","Krate","D","NucModifyRatio","TauRM","TauRO","CopyDrift","TableEnd"}}, + {"FitRegionFullNoRDR", {"R","Copies","Ampl","TShift","TMidNuc","Sigma","TMidNucDelay","SigmaMult","Krate","D","NucModifyRatio","TauRM","TauRO","CopyDrift","TableEnd"}}, + {"FitRegionTimeVarying",{"TMidNuc","RatioDrift","CopyDrift","TableEnd"}}, + {"FitRegionDarkness", {"Darkness","Ampl","TableEnd"}}, + //region-wide fits without diffusion + {"FitRegionInit2TauENoD", {"R","Copies","Ampl","TShift","TMidNuc","Sigma","Krate","NucModifyRatio","TauE","RatioDrift","CopyDrift","TableEnd"}}, + {"FitRegionInit2TauENoRDRNoD", {"R","Copies","Ampl","TShift","TMidNuc","Sigma","Krate","NucModifyRatio","TauE","CopyDrift","TableEnd"}}, + {"FitRegionFullTauENoD", {"R","Copies","Ampl","TShift","TMidNuc","Sigma","Krate","NucModifyRatio","TauE","RatioDrift","CopyDrift","TableEnd"}}, + {"FitRegionFullTauENoRDRNoD", {"R","Copies","Ampl","TShift","TMidNuc","Sigma","Krate","NucModifyRatio","TauE","CopyDrift","TableEnd"}}, + }; + + // set each entry for base_bkg_model_fit_type + for (auto p: fit_type_hash_table) + { + CreateBkgModelFitType(p.first, p.second); + base_bkg_model_fit_type[p.first].CreateBuildInstructions(_nucIdentity, max(_flowKey - _flowStart, 0 ), _flowBlockSize); + } +} + + +fit_descriptor master_fit_type_table::make_fit_param_entry(std::string param) { - for (int i=0;data[i].name != NULL;i++) - { - if (strcmp(data[i].name,name) == 0) - return &data[i].fi; + fit_descriptor fd; + if (param == "wellAmpl") fd = fit_descriptor({DFDA, & BeadParams::AccessAmpl, 0, ParamTypePerFlow }); + else if (param == "wellR") fd = fit_descriptor({DFDR, & BeadParams::AccessR, 0, ParamTypeAllFlow }); + else if (param == "wellCopies") fd = fit_descriptor({DFDP, & BeadParams::AccessCopies, 0, ParamTypeAllFlow }); + else if (param == "wellDmult") fd = fit_descriptor({DFDPDM, & BeadParams::AccessDmult, 0, ParamTypeAllFlow }); + else if (param == "wellKmult") fd = fit_descriptor({DFDDKR, & BeadParams::AccessKmult, 0, ParamTypePerFlow }); + else if (param == "wellAmplPostKey")fd = fit_descriptor({DFDA, & BeadParams::AccessAmplPostKey, 0, ParamTypeNotKey }); + + else if (param == "Ampl") fd = fit_descriptor({DFDA, 0, & reg_params::AccessAmpl, ParamTypePerFlow }); + else if (param == "R") fd = fit_descriptor({DFDR, 0, & reg_params::AccessR, ParamTypeAllFlow }); + else if (param == "Copies") fd = fit_descriptor({DFDP, 0, & reg_params::AccessCopies, ParamTypeAllFlow }); + else if (param == "D") fd = fit_descriptor({DFDD, 0, & reg_params::AccessD, ParamTypePerNuc }); + else if (param == "Darkness") fd = fit_descriptor({DFDERR, 0, & reg_params::AccessDarkness, ParamTypeAllFlow }); + else if (param == "TShift") fd = fit_descriptor({DFDTSH, 0, & reg_params::AccessTShift, ParamTypeAllFlow }); + else if (param == "TMidNuc") fd = fit_descriptor({DFDT0, 0, & reg_params::AccessTMidNuc, ParamTypeAllFlow }); + else if (param == "TMidNucDelay") fd = fit_descriptor({DFDT0DLY, 0, & reg_params::AccessTMidNucDelay,ParamTypePerNuc }); + else if (param == "Sigma") fd = fit_descriptor({DFDSIGMA, 0, & reg_params::AccessSigma, ParamTypeAllFlow }); + else if (param == "SigmaMult") fd = fit_descriptor({DFDSMULT, 0, & reg_params::AccessSigmaMult, ParamTypePerNuc }); + else if (param == "Krate") fd = fit_descriptor({DFDKRATE, 0, & reg_params::AccessKrate, ParamTypePerNuc }); + else if (param == "NucModifyRatio") fd = fit_descriptor({DFDMR, 0, & reg_params::AccessNucModifyRatio, ParamTypePerNuc }); + else if (param == "TauRM") fd = fit_descriptor({DFDTAUMR, 0, & reg_params::AccessTauRM, ParamTypeAllFlow }); + else if (param == "TauRO") fd = fit_descriptor({DFDTAUOR, 0, & reg_params::AccessTauRO, ParamTypeAllFlow }); + else if (param == "TauE") fd = fit_descriptor({DFDTAUE, 0, & reg_params::AccessTauE, ParamTypeAllFlow }); + else if (param == "RatioDrift") fd = fit_descriptor({DFDRDR, 0, & reg_params::AccessRatioDrift, ParamTypeAllFlow }); + else if (param == "CopyDrift") fd = fit_descriptor({DFDPDR, 0, & reg_params::AccessCopyDrift, ParamTypeAllFlow }); + else if (param == "TableEnd") fd = fit_descriptor({TBL_END, 0, 0, ParamTableEnd }); + else { + throw FitTypeException(param + ": Not a valid bead/reg param \n"); } - return NULL; + return fd; } -fit_descriptor *master_fit_type_table::GetFitDescriptorByName(const char *name) const +void master_fit_type_table::CreateBkgModelFitType(const string &fitName, const std::vector &fit_params) { + master_fit_type_entry mfte; + mfte.name = fitName.c_str(); + mfte.fds.resize(fit_params.size()); + for (size_t i=0; isecond.fi); + } + else { + pthread_mutex_unlock(&addFit); + throw FitTypeException(name + " :Not a valid fit type name\n"); } +} - return NULL; +const vector& master_fit_type_table::GetFitDescriptorByName(const string& name) +{ + pthread_mutex_lock(&addFit); + auto it = base_bkg_model_fit_type.find(name); + if (it != base_bkg_model_fit_type.end()) { + pthread_mutex_unlock(&addFit); + return it->second.fds; + } + else { + pthread_mutex_unlock(&addFit); + throw FitTypeException(name + " :Not a valid fit type name\n"); + } } @@ -446,38 +492,35 @@ master_fit_type_table::master_fit_type_table( int flow_start, int flow_key, int flow_block_size - ) + ) :_flowStart(flow_start), _flowKey(flow_key), _flowBlockSize(flow_block_size) { - int my_nuc_block[flow_block_size]; - tears.GetFlowOrderBlock( my_nuc_block, flow_start, flow_start + flow_block_size ); + _nucIdentity = new int[_flowBlockSize]; + tears.GetFlowOrderBlock(_nucIdentity, _flowStart, _flowStart + _flowBlockSize ); - // We want to copy the prototype table. - data = new master_fit_type_entry[ sizeof( base_bkg_model_fit_type ) / - sizeof( master_fit_type_entry ) ]; + // for thread safe adding of dynamic fit types during signal processing - // go through the master table of fit types and generate all the build - // instructions for each type of fitting we are going to do - for (int i=0 ; ; i++) - { - data[i] = base_bkg_model_fit_type[i]; + pthread_mutex_init(&addFit, NULL); - if ( base_bkg_model_fit_type[i].name == NULL ) break; + set_base_bkg_model_fit_type(); - data[i].CreateBuildInstructions(my_nuc_block, std::max( flow_key - flow_start, 0 ), flow_block_size); - } + // go through the master table of fit types and generate all the build + // instructions for each type of fitting we are going to do + + //for (auto it=base_bkg_model_fit_type.begin(); it != base_bkg_model_fit_type.end(); ++it) { + // it->second.CreateBuildInstructions(_nucIdentity, max(_flowKey - _flowStart, 0 ), _flowBlockSize); + //} } master_fit_type_table::~master_fit_type_table() { - for (int i=0;data[i].name != NULL;i++) - { - struct master_fit_type_entry *ft = &data[i]; + for (auto it=base_bkg_model_fit_type.begin(); it != base_bkg_model_fit_type.end(); ++it) { + master_fit_type_entry *ft = &(it->second); // make sure there is a high-level descriptor for this row // if there wasn't one, then the row might contain a hard link to // a statically allocated matrix build instruction which we don't // want to free - if (ft->fd != NULL) + if (ft->fds.size() > 0) { if (ft->mb != NULL) { @@ -496,8 +539,26 @@ master_fit_type_table::~master_fit_type_table() ft->fi.output = NULL; } - // Final cleanup. - delete [] data; + delete [] _nucIdentity; + pthread_mutex_destroy(&addFit); } +void master_fit_type_table::addBkgModelFitType( + const string& fitName, + const vector& paramNames) +{ + pthread_mutex_lock(&addFit); + try { + auto it = base_bkg_model_fit_type.find(fitName); + if (it == base_bkg_model_fit_type.end()) { + CreateBkgModelFitType(fitName, paramNames); + base_bkg_model_fit_type[fitName].CreateBuildInstructions(_nucIdentity, max(_flowKey - _flowStart, 0 ), _flowBlockSize); + } + } + catch(exception &ft) { + pthread_mutex_unlock(&addFit); + throw FitTypeException(ft.what()); + } + pthread_mutex_unlock(&addFit); +} diff --git a/Analysis/BkgModel/Fitters/Complex/BkgFitOptim.h b/Analysis/BkgModel/Fitters/Complex/BkgFitOptim.h index 926a5fcd..89f11bd6 100644 --- a/Analysis/BkgModel/Fitters/Complex/BkgFitOptim.h +++ b/Analysis/BkgModel/Fitters/Complex/BkgFitOptim.h @@ -4,12 +4,18 @@ #include #include +#include +#include +#include +#include +#include #include "BkgMagicDefines.h" #include "BkgFitMatrixPacker.h" #include "BeadParams.h" #include "RegionParams.h" #include "FlowDefaults.h" +using namespace std; typedef enum { @@ -25,6 +31,17 @@ typedef enum #define NOTNUCRISEPARAM ( float * ( nuc_rise_params::* )() )( 0 ) #define FIRSTINDEX 0 +class FitTypeException : public exception +{ + std::string msg; + + public: + FitTypeException(const std::string& s) : msg(s) {} + virtual ~FitTypeException() {} + + const char* what() const throw() { return msg.c_str(); } +}; + struct CpuStep { enum Length { @@ -66,7 +83,6 @@ class mat_table_build_instr mat_table_build_instr( const mat_table_build_instr & ); // Don't do this. mat_table_build_instr & operator=( const mat_table_build_instr & ); // Don't do this, either. - int * affected_flows; public: mat_table_build_instr() { @@ -76,13 +92,15 @@ class mat_table_build_instr bead_params_func = 0; reg_params_func = 0; } - ~mat_table_build_instr() { delete [] affected_flows; } + ~mat_table_build_instr() { if (affected_flows) delete [] affected_flows; } void realloc( int size ) { delete [] affected_flows; affected_flows = new int[size]; } + int * affected_flows; PartialDerivComponent comp; float * ( BeadParams::* bead_params_func )(); float * ( reg_params::* reg_params_func )(); int array_index; + std::string name; int GetAffectedFlow( int which ) const { return affected_flows[which]; } void SetAffectedFlow( int which, int value ) { affected_flows[which] = value; } @@ -115,17 +133,45 @@ struct fit_descriptor float * ( BeadParams::* bead_params_func )(); float * ( reg_params::* reg_params_func )(); ParameterSensitivityClassification ptype; + + fit_descriptor(PartialDerivComponent ncomp, float* (BeadParams::*bpf)(), float* (reg_params::*rpf)(), ParameterSensitivityClassification ntype) : comp(ncomp), bead_params_func(bpf), reg_params_func(rpf),ptype(ntype){} + + fit_descriptor() { + comp = TBL_END; + bead_params_func = NULL; + reg_params_func = NULL; + ptype = ParamTableEnd; + } + + fit_descriptor(const fit_descriptor& fd) { + comp = fd.comp; + bead_params_func = fd.bead_params_func; + reg_params_func = fd.reg_params_func; + ptype = fd.ptype; + } + + fit_descriptor& operator=(const fit_descriptor& fd) { + if (this != &fd) { + comp = fd.comp; + bead_params_func = fd.bead_params_func; + reg_params_func = fd.reg_params_func; + ptype = fd.ptype; + } + return *this; + } }; + struct master_fit_type_entry { // nice human-readable descriptive name for what the fit attempts to do - const char *name; + //const char *name; + std::string name; // high-level fit descriptor list. One entry in the list for each parameter to be // fit, along with a classification of the parameter that indicates whether it's one-per-flow // or one-per-nuc, etc.,... This high level description is used to build the // mat_table_build_instr table. - struct fit_descriptor *fd; + std::vector fds; // mid-level matrix build instructions. This intermediate level table contains multiple entries // for some parameters. (i.e., the Ampl parameter, which is independent per flow is broken out // in this table to one entry per flow, whereas it was a single line in the fit_descriptor....) @@ -141,19 +187,30 @@ struct master_fit_type_entry fit_instructions fi; void CreateBuildInstructions(const int *my_nuc, int flow_key, int flow_block_size); + + master_fit_type_entry(){ + mb = NULL; + } + + master_fit_type_entry(const master_fit_type_entry& mfte) : name(mfte.name),fds(mfte.fds),mb(mfte.mb),fi(mfte.fi){ + } + }; class master_fit_type_table { - // All of the table entries. - master_fit_type_entry *data; + int _flowStart; + int _flowKey; + int _flowBlockSize; + int *_nucIdentity; + std::map base_bkg_model_fit_type; + std::map > fit_type_hash_table; + pthread_mutex_t addFit; // No copying or assignment. master_fit_type_table( const master_fit_type_table & ); master_fit_type_table & operator=( const master_fit_type_table & ); - // The base data that we start with when making our array. - static master_fit_type_entry base_bkg_model_fit_type[]; public: // TODO: PartialDeriv 'affected flows' has to be munged for tango flow order!!! */ @@ -164,13 +221,18 @@ class master_fit_type_table // Cleanup! (Used to be CleanupLevMarSparseMatrices()). ~master_fit_type_table(); - fit_instructions *GetFitInstructionsByName(const char *name) const; - fit_descriptor *GetFitDescriptorByName(const char* name) const; -}; + fit_instructions *GetFitInstructionsByName(const std::string& name); + const std::vector& GetFitDescriptorByName(const std::string& name); + void addBkgModelFitType(const std::string &fitName, const std::vector &fit_params); +private: + void set_base_bkg_model_fit_type(); + fit_descriptor make_fit_param_entry(std::string param); + void CreateBkgModelFitType(const std::string &fitName, const std::vector &fit_params); +}; -void InitializeLevMarFitter(mat_table_build_instr *btbl,fit_instructions *instr, int flow_block_size); +void InitializeLevMarFitter(const mat_table_build_instr *btbl,fit_instructions *instr, int flow_block_size); void DumpBuildInstructionTable(mat_table_build_instr *tbl, int flow_block_size); #endif // BKGFITOPTIM_H diff --git a/Analysis/BkgModel/Fitters/Complex/BkgFitStructures.cpp b/Analysis/BkgModel/Fitters/Complex/BkgFitStructures.cpp index 159167b0..7a2b85af 100644 --- a/Analysis/BkgModel/Fitters/Complex/BkgFitStructures.cpp +++ b/Analysis/BkgModel/Fitters/Complex/BkgFitStructures.cpp @@ -1,5 +1,8 @@ /* Copyright (C) 2010 Ion Torrent Systems, Inc. All Rights Reserved */ +#include +#include +#include #include "BkgFitStructures.h" using namespace std; @@ -53,7 +56,7 @@ CpuStep BkgFitStructures::Steps[] = int BkgFitStructures::NumSteps = sizeof(Steps) /sizeof(Steps[FIRSTINDEX]); - +/* fit_descriptor BkgFitStructures::fit_well_ampl_descriptor[] = { //{PartialDerivComponent, bead_params_func, reg_params_func, ParameterSensitivityClassification} @@ -454,48 +457,39 @@ fit_descriptor BkgFitStructures::fit_region_full_taue_NoRDR_NoD_descriptor[] = {TBL_END, 0, 0, ParamTableEnd }, }; -int BkgFitStructures::GetNumParamsToFitForDescriptor(fit_descriptor *fd, int flow_key, int flow_block_size) +master_fit_type_entry master_fit_type_table::base_bkg_model_fit_type[] = { - int numParamsToFit = 0; - for (int i=0; fd[i].comp != TBL_END; ++i) - { - switch (fd[i].ptype) - { - case ParamTypeAFlows: - case ParamTypeCFlows: - case ParamTypeGFlows: - case ParamTypeAllFlow: - numParamsToFit++; - break; - case ParamTypeNotKey: - numParamsToFit += max( 0, flow_block_size-flow_key ); - break; - case ParamTypePerFlow: - numParamsToFit += flow_block_size; - break; - case ParamTypePerNuc: - numParamsToFit += NUMNUC; - break; - case ParamTypeAllButFlow0: - numParamsToFit += flow_block_size-1; - break; - default: - break; - } - } - return numParamsToFit; -} +//{"name", &fit_descriptor, NULL, {NULL,0,NULL,0}}, + // individual well fits + {"FitWellAmpl", BkgFitStructures::fit_well_ampl_descriptor, NULL, {NULL,0,NULL,0}}, + {"FitWellAmplBuffering", BkgFitStructures::fit_well_ampl_buffering_descriptor, NULL, {NULL,0,NULL,0}}, + {"FitWellPostKey", BkgFitStructures::fit_well_post_key_descriptor, NULL, {NULL,0,NULL,0}}, + {"FitWellPostKeyNoDmult", BkgFitStructures::fit_well_post_key_descriptor_nodmult, NULL, {NULL,0,NULL,0}}, + + // region-wide fits + {"FitRegionTmidnucPlus", BkgFitStructures::fit_region_tmidnuc_plus_descriptor, NULL, {NULL,0,NULL,0}}, + {"FitRegionInit2", BkgFitStructures::fit_region_init2_descriptor, NULL, {NULL,0,NULL,0}}, + {"FitRegionInit2TauE", BkgFitStructures::fit_region_init2_taue_descriptor, NULL, {NULL,0,NULL,0}}, + {"FitRegionInit2TauENoRDR", BkgFitStructures::fit_region_init2_taue_NoRDR_descriptor, NULL, {NULL,0,NULL,0}}, + {"FitRegionFull", BkgFitStructures::fit_region_full_descriptor, NULL, {NULL,0,NULL,0}}, + {"FitRegionFullTauE", BkgFitStructures::fit_region_full_taue_descriptor, NULL, {NULL,0,NULL,0}}, + {"FitRegionFullTauENoRDR", BkgFitStructures::fit_region_full_taue_NoRDR_descriptor, NULL, {NULL,0,NULL,0}}, + {"FitRegionInit2NoRDR", BkgFitStructures::fit_region_init2_noRatioDrift_descriptor, NULL, {NULL,0,NULL,0}}, + {"FitRegionFullNoRDR", BkgFitStructures::fit_region_full_noRatioDrift_descriptor, NULL, {NULL,0,NULL,0}}, + {"FitRegionTimeVarying", BkgFitStructures::fit_region_time_varying_descriptor, NULL, {NULL,0,NULL,0}}, + {"FitRegionDarkness", BkgFitStructures::fit_region_darkness_descriptor, NULL, {NULL,0,NULL,0}}, + + //region-wide fits without diffusion + {"FitRegionInit2TauENoD", BkgFitStructures::fit_region_init2_taue_NoD_descriptor, NULL, {NULL,0,NULL,0}}, + {"FitRegionInit2TauENoRDRNoD",BkgFitStructures::fit_region_init2_taue_NoRDR_NoD_descriptor, NULL, {NULL,0,NULL,0}}, + {"FitRegionFullTauENoD", BkgFitStructures::fit_region_full_taue_NoD_descriptor, NULL, {NULL,0,NULL,0}}, + {"FitRegionFullTauENoRDRNoD", BkgFitStructures::fit_region_full_taue_NoRDR_NoD_descriptor, NULL, {NULL,0,NULL,0}}, + + { NULL, NULL, NULL, {NULL,0,NULL,0} }, // end of table +}; +*/ -int BkgFitStructures::GetNumParDerivStepsForFitDescriptor(fit_descriptor* fd) { - int numParDerivSteps = 0; - for (int i=0; fd[i].comp != TBL_END; ++i) - { - numParDerivSteps++; - } - return numParDerivSteps; -} -//#define NUMERIC_PartialDeriv_CALC void BuildMatrix(BkgFitMatrixPacker *fit,bool accum, bool debug) { #if 1 @@ -535,4 +529,50 @@ void BuildMatrix(BkgFitMatrixPacker *fit,bool accum, bool debug) #endif } +int BkgFitStructures::GetNumParamsToFitForDescriptor( + const std::vector& fds, + int flow_key, + int flow_block_size) +{ + int numParamsToFit = 0; + if (fds.size() > 0) { + for (int i=0; fds[i].comp != TBL_END; ++i) + { + switch (fds[i].ptype) + { + case ParamTypeAFlows: + case ParamTypeCFlows: + case ParamTypeGFlows: + case ParamTypeAllFlow: + numParamsToFit++; + break; + case ParamTypeNotKey: + numParamsToFit += max( 0, flow_block_size-flow_key ); + break; + case ParamTypePerFlow: + numParamsToFit += flow_block_size; + break; + case ParamTypePerNuc: + numParamsToFit += NUMNUC; + break; + case ParamTypeAllButFlow0: + numParamsToFit += flow_block_size-1; + break; + default: + break; + } + } + } + return numParamsToFit; +} +int BkgFitStructures::GetNumParDerivStepsForFitDescriptor(const std::vector& fds) { + int numParDerivSteps = 0; + if (fds.size() > 0) { + for (int i=0; fds[i].comp != TBL_END; ++i) + { + numParDerivSteps++; + } + } + return numParDerivSteps; +} diff --git a/Analysis/BkgModel/Fitters/Complex/BkgFitStructures.h b/Analysis/BkgModel/Fitters/Complex/BkgFitStructures.h index f8583e91..a78bbc9b 100644 --- a/Analysis/BkgModel/Fitters/Complex/BkgFitStructures.h +++ b/Analysis/BkgModel/Fitters/Complex/BkgFitStructures.h @@ -12,6 +12,7 @@ class BkgFitStructures{ static CpuStep Steps[]; static int NumSteps; + /* static fit_descriptor fit_well_ampl_descriptor[]; static fit_descriptor fit_well_ampl_buffering_descriptor[]; static fit_descriptor fit_well_post_key_descriptor[]; @@ -39,8 +40,35 @@ class BkgFitStructures{ static fit_descriptor fit_region_full_taue_NoD_descriptor[]; static fit_descriptor fit_region_full_taue_NoRDR_NoD_descriptor[]; - static int GetNumParamsToFitForDescriptor(fit_descriptor *fd, int flow_key, int flow_block_size); - static int GetNumParDerivStepsForFitDescriptor(fit_descriptor* fd); + std::vector fit_well_ampl_descriptor; + std::vector fit_well_ampl_buffering_descriptor; + std::vector fit_well_post_key_descriptor; + std::vector fit_well_post_key_descriptor_nodmult; + + // region + std::vector fit_region_tmidnuc_plus_descriptor; + + std::vector fit_region_init2_descriptor; + std::vector fit_region_init2_taue_descriptor; + std::vector fit_region_init2_noRatioDrift_descriptor; + std::vector fit_region_init2_taue_NoRDR_descriptor; + + std::vector fit_region_full_descriptor; + std::vector fit_region_full_taue_descriptor; + std::vector fit_region_full_noRatioDrift_descriptor; + std::vector fit_region_full_taue_NoRDR_descriptor; + + std::vector fit_region_time_varying_descriptor; + std::vector fit_region_darkness_descriptor; + + std::vector fit_region_init2_taue_NoD_descriptor; + std::vector fit_region_init2_taue_NoRDR_NoD_descriptor; + std::vector fit_region_full_taue_NoD_descriptor; + std::vector fit_region_full_taue_NoRDR_NoD_descriptor; + */ + + static int GetNumParamsToFitForDescriptor(const std::vector& fds, int flow_key, int flow_block_size); + static int GetNumParDerivStepsForFitDescriptor(const std::vector& fds); }; diff --git a/Analysis/BkgModel/Fitters/Complex/FitControl.cpp b/Analysis/BkgModel/Fitters/Complex/FitControl.cpp index 22ae3466..259cee98 100644 --- a/Analysis/BkgModel/Fitters/Complex/FitControl.cpp +++ b/Analysis/BkgModel/Fitters/Complex/FitControl.cpp @@ -9,11 +9,12 @@ FitControl_t::FitControl_t( master_fit_type_table *table ) : { PartialDeriv_comp_list = NULL; // well - NullWellPackers(); + //NullWellPackers(); // region - NullRegionPackers(); + //NullRegionPackers(); } +/* void FitControl_t::NullWellPackers() { FitWellAmpl = NULL; @@ -22,8 +23,8 @@ void FitControl_t::NullWellPackers() DontFitWells = NULL; FitWellAll = NULL; } - -void FitControl_t::NullRegionPackers() +*/ +/*void FitControl_t::NullRegionPackers() { FitRegionTmidnucPlus= NULL; FitRegionInit2= NULL; @@ -54,13 +55,18 @@ void FitControl_t::DeleteRegionPackers() if ( DontFitRegion !=NULL ) delete DontFitRegion; // should never happen!!! NullRegionPackers(); } +*/ void FitControl_t::Delete() { - DeleteWellPackers(); + //DeleteWellPackers(); - DeleteRegionPackers(); + //DeleteRegionPackers(); + for(auto it = fittingMatrices.begin(); it!= fittingMatrices.end(); ++it) { + delete it->second; + } + fittingMatrices.clear(); if ( PartialDeriv_comp_list != NULL ) delete [] PartialDeriv_comp_list; } @@ -72,24 +78,30 @@ FitControl_t::~FitControl_t() void FitControl_t::AllocWellPackers ( int hydrogenModelType, int npts, int flow_block_size ) { - DeleteWellPackers(); + //DeleteWellPackers(); - int comp_list_len = BkgFitStructures::NumSteps; + //int comp_list_len = BkgFitStructures::NumSteps; + + AddFitPacker("FitWellAmpl", npts, flow_block_size); + //FitWellAmpl = new BkgFitMatrixPacker ( npts,*bkg_model_fit_type->GetFitInstructionsByName ( "FitWellAmpl" ),PartialDeriv_comp_list,comp_list_len, flow_block_size ); + //FitWellAmplBuffering = new BkgFitMatrixPacker ( npts,*bkg_model_fit_type->GetFitInstructionsByName ( "FitWellAmplBuffering" ),PartialDeriv_comp_list,comp_list_len, flow_block_size ); + AddFitPacker("FitWellAmplBuffering", npts, flow_block_size); - FitWellAmpl = new BkgFitMatrixPacker ( npts,*bkg_model_fit_type->GetFitInstructionsByName ( "FitWellAmpl" ),PartialDeriv_comp_list,comp_list_len, flow_block_size ); - FitWellAmplBuffering = new BkgFitMatrixPacker ( npts,*bkg_model_fit_type->GetFitInstructionsByName ( "FitWellAmplBuffering" ),PartialDeriv_comp_list,comp_list_len, flow_block_size ); switch( hydrogenModelType ){ case 0: default: - FitWellPostKey = new BkgFitMatrixPacker ( npts,*bkg_model_fit_type->GetFitInstructionsByName ( "FitWellPostKey" ),PartialDeriv_comp_list,comp_list_len, flow_block_size ); + //FitWellPostKey = new BkgFitMatrixPacker ( npts,*bkg_model_fit_type->GetFitInstructionsByName ( "FitWellPostKey" ),PartialDeriv_comp_list,comp_list_len, flow_block_size ); + AddFitPacker("FitWellPostKey", npts, flow_block_size); break; case 1: case 2: case 3: - FitWellPostKey = new BkgFitMatrixPacker ( npts,*bkg_model_fit_type->GetFitInstructionsByName ( "FitWellPostKeyNoDmult" ),PartialDeriv_comp_list,comp_list_len, flow_block_size ); + //FitWellPostKey = new BkgFitMatrixPacker ( npts,*bkg_model_fit_type->GetFitInstructionsByName ( "FitWellPostKeyNoDmult" ),PartialDeriv_comp_list,comp_list_len, flow_block_size ); + AddFitPacker("FitWellPostKeyNoDmult", npts, flow_block_size); break; } - FitWellAll = new BkgFitMatrixPacker ( npts,*bkg_model_fit_type->GetFitInstructionsByName ( "FitWellAll" ),PartialDeriv_comp_list,comp_list_len, flow_block_size ); + //FitWellAll = new BkgFitMatrixPacker ( npts,*bkg_model_fit_type->GetFitInstructionsByName ( "FitWellAll" ),PartialDeriv_comp_list,comp_list_len, flow_block_size ); + AddFitPacker("FitWellAll", npts, flow_block_size); } @@ -103,26 +115,34 @@ void FitControl_t::CombinatorialAllocationOfInit2AndFull ( bool no_rdr_fit_start if ( no_rdr_fit_starting_block & ( !fitting_taue ) ) { - FitRegionInit2 = new BkgFitMatrixPacker ( npts,*bkg_model_fit_type->GetFitInstructionsByName ( "FitRegionInit2NoRDR" ),PartialDeriv_comp_list,comp_list_len, flow_block_size ); - FitRegionFull = new BkgFitMatrixPacker ( npts,*bkg_model_fit_type->GetFitInstructionsByName ( "FitRegionFullNoRDR" ),PartialDeriv_comp_list,comp_list_len, flow_block_size ); + //FitRegionInit2 = new BkgFitMatrixPacker ( npts,*bkg_model_fit_type->GetFitInstructionsByName ( "FitRegionInit2NoRDR" ),PartialDeriv_comp_list,comp_list_len, flow_block_size ); + AddFitPacker("FitRegionInit2NoRDR",npts,flow_block_size); + //FitRegionFull = new BkgFitMatrixPacker ( npts,*bkg_model_fit_type->GetFitInstructionsByName ( "FitRegionFullNoRDR" ),PartialDeriv_comp_list,comp_list_len, flow_block_size ); + AddFitPacker("FitRegionFullNoRDR",npts,flow_block_size); } if ( ( !no_rdr_fit_starting_block ) & ( !fitting_taue ) ) { - FitRegionInit2 = new BkgFitMatrixPacker ( npts,*bkg_model_fit_type->GetFitInstructionsByName ( "FitRegionInit2" ),PartialDeriv_comp_list,comp_list_len, flow_block_size ); - FitRegionFull = new BkgFitMatrixPacker ( npts,*bkg_model_fit_type->GetFitInstructionsByName ( "FitRegionFull" ),PartialDeriv_comp_list,comp_list_len, flow_block_size ); + //FitRegionInit2 = new BkgFitMatrixPacker ( npts,*bkg_model_fit_type->GetFitInstructionsByName ( "FitRegionInit2" ),PartialDeriv_comp_list,comp_list_len, flow_block_size ); + AddFitPacker("FitRegionInit2",npts,flow_block_size); + //FitRegionFull = new BkgFitMatrixPacker ( npts,*bkg_model_fit_type->GetFitInstructionsByName ( "FitRegionFull" ),PartialDeriv_comp_list,comp_list_len, flow_block_size ); + AddFitPacker("FitRegionFull",npts,flow_block_size); } if ( fitting_taue & ( !no_rdr_fit_starting_block ) ) { - FitRegionInit2 = new BkgFitMatrixPacker ( npts,*bkg_model_fit_type->GetFitInstructionsByName ( "FitRegionInit2TauE" ),PartialDeriv_comp_list,comp_list_len, flow_block_size ); - FitRegionFull = new BkgFitMatrixPacker ( npts,*bkg_model_fit_type->GetFitInstructionsByName ( "FitRegionFullTauE" ),PartialDeriv_comp_list,comp_list_len, flow_block_size ); + //FitRegionInit2 = new BkgFitMatrixPacker ( npts,*bkg_model_fit_type->GetFitInstructionsByName ( "FitRegionInit2TauE" ),PartialDeriv_comp_list,comp_list_len, flow_block_size ); + AddFitPacker("FitRegionInit2TauE",npts,flow_block_size); + //FitRegionFull = new BkgFitMatrixPacker ( npts,*bkg_model_fit_type->GetFitInstructionsByName ( "FitRegionFullTauE" ),PartialDeriv_comp_list,comp_list_len, flow_block_size ); + AddFitPacker("FitRegionFullTauE",npts,flow_block_size); } if ( fitting_taue & ( no_rdr_fit_starting_block ) ) { - FitRegionInit2 = new BkgFitMatrixPacker ( npts,*bkg_model_fit_type->GetFitInstructionsByName ( "FitRegionInit2TauENoRDR" ),PartialDeriv_comp_list,comp_list_len, flow_block_size ); - FitRegionFull = new BkgFitMatrixPacker ( npts,*bkg_model_fit_type->GetFitInstructionsByName ( "FitRegionFullTauENoRDR" ),PartialDeriv_comp_list,comp_list_len, flow_block_size ); + //FitRegionInit2 = new BkgFitMatrixPacker ( npts,*bkg_model_fit_type->GetFitInstructionsByName ( "FitRegionInit2TauENoRDR" ),PartialDeriv_comp_list,comp_list_len, flow_block_size ); + AddFitPacker("FitRegionInit2TauENoRDR",npts,flow_block_size); + //FitRegionFull = new BkgFitMatrixPacker ( npts,*bkg_model_fit_type->GetFitInstructionsByName ( "FitRegionFullTauENoRDR" ),PartialDeriv_comp_list,comp_list_len, flow_block_size ); + AddFitPacker("FitRegionFullTauENoRDR",npts,flow_block_size); } break; @@ -131,14 +151,18 @@ void FitControl_t::CombinatorialAllocationOfInit2AndFull ( bool no_rdr_fit_start case 3: if ( fitting_taue & ( !no_rdr_fit_starting_block ) ) { - FitRegionInit2 = new BkgFitMatrixPacker ( npts,*bkg_model_fit_type->GetFitInstructionsByName ( "FitRegionInit2TauENoD" ),PartialDeriv_comp_list,comp_list_len, flow_block_size ); - FitRegionFull = new BkgFitMatrixPacker ( npts,*bkg_model_fit_type->GetFitInstructionsByName ( "FitRegionFullTauENoD" ),PartialDeriv_comp_list,comp_list_len, flow_block_size ); + //FitRegionInit2 = new BkgFitMatrixPacker ( npts,*bkg_model_fit_type->GetFitInstructionsByName ( "FitRegionInit2TauENoD" ),PartialDeriv_comp_list,comp_list_len, flow_block_size ); + AddFitPacker("FitRegionInit2TauENoD",npts,flow_block_size); + //FitRegionFull = new BkgFitMatrixPacker ( npts,*bkg_model_fit_type->GetFitInstructionsByName ( "FitRegionFullTauENoD" ),PartialDeriv_comp_list,comp_list_len, flow_block_size ); + AddFitPacker("FitRegionFullTauENoD",npts,flow_block_size); } if ( fitting_taue & ( no_rdr_fit_starting_block ) ) { - FitRegionInit2 = new BkgFitMatrixPacker ( npts,*bkg_model_fit_type->GetFitInstructionsByName ( "FitRegionInit2TauENoRDRNoD" ),PartialDeriv_comp_list,comp_list_len, flow_block_size ); - FitRegionFull = new BkgFitMatrixPacker ( npts,*bkg_model_fit_type->GetFitInstructionsByName ( "FitRegionFullTauENoRDRNoD" ),PartialDeriv_comp_list,comp_list_len, flow_block_size ); + //FitRegionInit2 = new BkgFitMatrixPacker ( npts,*bkg_model_fit_type->GetFitInstructionsByName ( "FitRegionInit2TauENoRDRNoD" ),PartialDeriv_comp_list,comp_list_len, flow_block_size ); + AddFitPacker("FitRegionInit2TauENoRDRNoD",npts,flow_block_size); + //FitRegionFull = new BkgFitMatrixPacker ( npts,*bkg_model_fit_type->GetFitInstructionsByName ( "FitRegionFullTauENoRDRNoD" ),PartialDeriv_comp_list,comp_list_len, flow_block_size ); + AddFitPacker("FitRegionFullTauENoRDRNoD",npts,flow_block_size); } break; } @@ -150,13 +174,16 @@ void FitControl_t::AllocRegionPackers ( bool no_rdr_fit_starting_block,bool fitt { int comp_list_len = BkgFitStructures::NumSteps; - DeleteRegionPackers(); - FitRegionTmidnucPlus = new BkgFitMatrixPacker ( npts,*bkg_model_fit_type->GetFitInstructionsByName ( "FitRegionTmidnucPlus" ),PartialDeriv_comp_list,comp_list_len, flow_block_size ); + //DeleteRegionPackers(); + //FitRegionTmidnucPlus = new BkgFitMatrixPacker ( npts,*bkg_model_fit_type->GetFitInstructionsByName ( "FitRegionTmidnucPlus" ),PartialDeriv_comp_list,comp_list_len, flow_block_size ); + AddFitPacker("FitRegionTmidnucPlus",npts,flow_block_size); CombinatorialAllocationOfInit2AndFull ( no_rdr_fit_starting_block,fitting_taue,hydrogenModelType,npts, flow_block_size ); - FitRegionTimeVarying = new BkgFitMatrixPacker ( npts,*bkg_model_fit_type->GetFitInstructionsByName ( "FitRegionTimeVarying" ),PartialDeriv_comp_list,comp_list_len, flow_block_size ); - FitRegionDarkness = new BkgFitMatrixPacker ( npts,*bkg_model_fit_type->GetFitInstructionsByName ( "FitRegionDarkness" ),PartialDeriv_comp_list,comp_list_len, flow_block_size ); + //FitRegionTimeVarying = new BkgFitMatrixPacker ( npts,*bkg_model_fit_type->GetFitInstructionsByName ( "FitRegionTimeVarying" ),PartialDeriv_comp_list,comp_list_len, flow_block_size ); + AddFitPacker("FitRegionTimeVarying",npts,flow_block_size); + //FitRegionDarkness = new BkgFitMatrixPacker ( npts,*bkg_model_fit_type->GetFitInstructionsByName ( "FitRegionDarkness" ),PartialDeriv_comp_list,comp_list_len, flow_block_size ); + AddFitPacker("FitRegionDarkness",npts,flow_block_size); } void FitControl_t::AllocPackers (float *tfptr, bool no_rdr_fit_starting_block, bool fitting_taue, int hydrogenModelType, int bead_flow_t, int npts, int flow_block_size ) @@ -173,3 +200,28 @@ void FitControl_t::AllocPackers (float *tfptr, bool no_rdr_fit_starting_block, b AllocWellPackers ( hydrogenModelType, npts, flow_block_size ); AllocRegionPackers ( no_rdr_fit_starting_block, fitting_taue, hydrogenModelType, npts , flow_block_size); } + +bool FitControl_t::AddFitPacker( + const char* fitName, + int numFrames, + int flow_block_size) +{ + BkgFitMatrixPacker* packer = new BkgFitMatrixPacker (numFrames,*bkg_model_fit_type->GetFitInstructionsByName (fitName),PartialDeriv_comp_list,BkgFitStructures::NumSteps, flow_block_size); + auto it = fittingMatrices.find(fitName); + if (it != fittingMatrices.end()) { + return false; + } + else { + fittingMatrices[fitName] = packer; + } + + return true; +} + +BkgFitMatrixPacker* FitControl_t::GetFitPacker(const string& fitName) const { + auto it = fittingMatrices.find(fitName); + if (it != fittingMatrices.end()) + return it->second; + else + return NULL; +} diff --git a/Analysis/BkgModel/Fitters/Complex/FitControl.h b/Analysis/BkgModel/Fitters/Complex/FitControl.h index d62ba3de..79ecce49 100644 --- a/Analysis/BkgModel/Fitters/Complex/FitControl.h +++ b/Analysis/BkgModel/Fitters/Complex/FitControl.h @@ -2,46 +2,30 @@ #ifndef FITCONTROL_H #define FITCONTROL_H +#include +#include #include "BkgFitStructures.h" +using namespace std; -// this should be a map rather than individual pointers -// it is 2011 and the STL has been in existencde for some time class FitControl_t { - master_fit_type_table* bkg_model_fit_type; - public: - // helper class to manipulate PartialDerivriv terms and solve the non-linear - PartialDeriv_comp_list_item *PartialDeriv_comp_list; - // regression calculation - BkgFitMatrixPacker *FitWellAmpl; - BkgFitMatrixPacker *FitWellAmplBuffering; - BkgFitMatrixPacker *FitWellPostKey; - BkgFitMatrixPacker *FitWellAll; // includes kmult - BkgFitMatrixPacker *DontFitWells; - - // used for fitting tau, NucModifyRatio and oR to the whole region, not just individual wells - BkgFitMatrixPacker *FitRegionTmidnucPlus; - BkgFitMatrixPacker *FitRegionInit2; - BkgFitMatrixPacker *FitRegionFull; - BkgFitMatrixPacker *FitRegionDarkness; - BkgFitMatrixPacker *FitRegionTimeVarying; - BkgFitMatrixPacker *DontFitRegion; + master_fit_type_table* bkg_model_fit_type; + map fittingMatrices; + PartialDeriv_comp_list_item *PartialDeriv_comp_list; + public: FitControl_t( master_fit_type_table *table ); - void Delete(); - void DeleteWellPackers(); - void DeleteRegionPackers(); - void NullWellPackers(); - void NullRegionPackers(); ~FitControl_t(); + void AllocPackers(float *tfptr, bool no_rdr_fit_starting_block,bool fitting_taue, int hydrogenModelType, int bead_flow_t, int npts, int flow_block_size); + bool AddFitPacker(const char* fitName, int numFrames, int flow_block_size); + BkgFitMatrixPacker* GetFitPacker(const string& fitName) const; + + private: void AllocWellPackers(int hydrogenModelType, int npts, int flow_block_size); void AllocRegionPackers(bool no_rdr_fit_starting_block, bool fitting_taue, int hydrogenModelType, int npts, int flow_block_size); void CombinatorialAllocationOfInit2AndFull(bool no_rdr_fit_starting_block, bool fitting_taue, int hydrogenModelType, int npts, int flow_block_size); - void AllocPackers(float *tfptr, bool no_rdr_fit_starting_block,bool fitting_taue, int hydrogenModelType, int bead_flow_t, int npts, int flow_block_size); + void Delete(); }; - - - #endif // FITCONTROL_H diff --git a/Analysis/BkgModel/Fitters/Complex/MultiLevMar.cpp b/Analysis/BkgModel/Fitters/Complex/MultiLevMar.cpp index f6efd9a8..c4419b1f 100644 --- a/Analysis/BkgModel/Fitters/Complex/MultiLevMar.cpp +++ b/Analysis/BkgModel/Fitters/Complex/MultiLevMar.cpp @@ -2,6 +2,7 @@ #include "MultiLevMar.h" #include "MiscVec.h" #include +#include "BkgFitMatDat.h" MultiFlowLevMar::MultiFlowLevMar ( SignalProcessingMasterFitter &_bkg, int flow_block_size, master_fit_type_table *table ) : @@ -66,7 +67,8 @@ int MultiFlowLevMar::MultiFlowSpecializedSampledLevMarFitParameters ( int additi { for ( int loc_iter=0 ; ( loc_iter< number_region_iterations_wanted ) & do_just_region; loc_iter++ ) { - DoSampledRegionIteration ( reg_fit,total_iter, flow_key, flow_block_size, flow_block_start ); + int nbeads = DoSampledRegionIteration ( reg_fit,total_iter, flow_key, flow_block_size, flow_block_start ); + IF_OPTIMIZER_DEBUG(bkg.inception_state, bkg.debugSaver.WriteData(reg_fit, bkg.region_data->my_regions.rp ,flow_block_start, bkg.GetRegion(),reg_fit->compNames, nbeads)); total_iter++; } } @@ -90,7 +92,8 @@ int MultiFlowLevMar::MultiFlowSpecializedSampledLevMarFitParameters ( int additi // do one region iteration if ( !skip_region ) { - DoSampledRegionIteration ( reg_fit,total_iter, flow_key, flow_block_size, flow_block_start ); + int nbeads = DoSampledRegionIteration ( reg_fit,total_iter, flow_key, flow_block_size, flow_block_start ); + IF_OPTIMIZER_DEBUG(bkg.inception_state, bkg.debugSaver.WriteData(reg_fit, bkg.region_data->my_regions.rp ,flow_block_start, bkg.GetRegion(),reg_fit->compNames, nbeads)); total_iter++; } } @@ -129,7 +132,8 @@ int MultiFlowLevMar::MultiFlowSpecializedLevMarFitParameters ( int additional_be { for ( int loc_iter=0 ; loc_itermy_regions.rp ,flow_block_start, bkg.GetRegion(),reg_fit->compNames, nbeads)); total_iter++; } } @@ -150,7 +154,8 @@ int MultiFlowLevMar::MultiFlowSpecializedLevMarFitParameters ( int additional_be // do one region iteration if ( !skip_region ) { - DoRegionIteration ( reg_fit, total_iter, flow_key, flow_block_size, flow_block_start ); + int nbeads = DoRegionIteration ( reg_fit, total_iter, flow_key, flow_block_size, flow_block_start ); + IF_OPTIMIZER_DEBUG(bkg.inception_state, bkg.debugSaver.WriteData(reg_fit, bkg.region_data->my_regions.rp ,flow_block_start, bkg.GetRegion(),reg_fit->compNames, nbeads)); total_iter++; } } @@ -186,7 +191,8 @@ int MultiFlowLevMar::MultiFlowSpecializedLevMarFitParametersOnlyRegion ( { for ( int loc_iter=0 ; loc_itermy_regions.rp ,flow_block_start, bkg.GetRegion(),reg_fit->compNames, nbeads)); total_iter++; } } @@ -212,7 +218,7 @@ void MultiFlowLevMar::MultiFlowSpecializedLevMarFitAllWells ( int bead_only_iter } ///-----------------------------------done with entry points -void MultiFlowLevMar::DoSampledRegionIteration ( +int MultiFlowLevMar::DoSampledRegionIteration ( BkgFitMatrixPacker *reg_fit, int iter, int flow_key, int flow_block_size, int flow_block_start ) { @@ -231,9 +237,10 @@ void MultiFlowLevMar::DoSampledRegionIteration ( printf("DoSampledRegionIteration: %d beads less than minimum %d required in region(col=%d,row=%d)\n",reg_wells, lm_state.min_bead_to_fit_region, bkg.region_data->region->col, bkg.region_data->region->row); } IdentifyParametersFromSample ( bkg.region_data->my_beads,bkg.region_data->my_regions, lm_state.well_mask, lm_state.reg_mask, lm_state , flow_block_size); + return reg_wells; } -void MultiFlowLevMar::DoRegionIteration ( +int MultiFlowLevMar::DoRegionIteration ( BkgFitMatrixPacker *reg_fit, int iter, int flow_key, int flow_block_size, int flow_block_start ) { @@ -257,6 +264,7 @@ void MultiFlowLevMar::DoRegionIteration ( lm_state.IncrementRegionGroup(); // better try another bead set if this one doesn't work! } IdentifyParameters ( bkg.region_data->my_beads,bkg.region_data->my_regions, *bkg.region_data_extras.my_flow, flow_block_size, lm_state.well_mask, lm_state.reg_mask,lm_state.skip_beads ); + return reg_wells; } diff --git a/Analysis/BkgModel/Fitters/Complex/MultiLevMar.h b/Analysis/BkgModel/Fitters/Complex/MultiLevMar.h index 9c096cb8..51a0161e 100644 --- a/Analysis/BkgModel/Fitters/Complex/MultiLevMar.h +++ b/Analysis/BkgModel/Fitters/Complex/MultiLevMar.h @@ -75,10 +75,10 @@ class MultiFlowLevMar bool isSample, int flow_key, int flow_block_size, int flow_block_start ); - void DoSampledRegionIteration ( BkgFitMatrixPacker *reg_fit, + int DoSampledRegionIteration ( BkgFitMatrixPacker *reg_fit, int iter, int flow_key, int flow_block_size, int flow_block_start ); - void DoRegionIteration ( BkgFitMatrixPacker *reg_fit, + int DoRegionIteration( BkgFitMatrixPacker *reg_fit, int iter, int flow_key, int flow_block_size, int flow_block_start ); bool DoSampledBeadIteration ( bool well_only_fit, diff --git a/Analysis/LevMarFitterV2.cpp b/Analysis/BkgModel/Fitters/LevMarFitterV2.cpp similarity index 100% rename from Analysis/LevMarFitterV2.cpp rename to Analysis/BkgModel/Fitters/LevMarFitterV2.cpp diff --git a/Analysis/LevMarFitterV2.h b/Analysis/BkgModel/Fitters/LevMarFitterV2.h similarity index 100% rename from Analysis/LevMarFitterV2.h rename to Analysis/BkgModel/Fitters/LevMarFitterV2.h diff --git a/Analysis/BkgModel/Fitters/SpatialCorrelator.cpp b/Analysis/BkgModel/Fitters/SpatialCorrelator.cpp index d3626e10..268826b5 100644 --- a/Analysis/BkgModel/Fitters/SpatialCorrelator.cpp +++ b/Analysis/BkgModel/Fitters/SpatialCorrelator.cpp @@ -11,7 +11,7 @@ #include "SignalProcessingMasterFitter.h" #include "RawWells.h" #include "MathOptim.h" -#include "mixed.h" +//#include "ClonalFilter/mixed.h" #include "BkgDataPointers.h" diff --git a/Analysis/BkgModel/GlobalDefaultsForBkgModel.cpp b/Analysis/BkgModel/GlobalDefaultsForBkgModel.cpp index 5db2c96c..524c008f 100644 --- a/Analysis/BkgModel/GlobalDefaultsForBkgModel.cpp +++ b/Analysis/BkgModel/GlobalDefaultsForBkgModel.cpp @@ -207,9 +207,8 @@ void GlobalDefaultsForBkgModel::GoptDefaultsFromJson(char *fname){ } - // Load optimized defaults from GeneticOptimizer runs -void GlobalDefaultsForBkgModel::SetGoptDefaults ( char *fname ) +void GlobalDefaultsForBkgModel::SetGoptDefaults ( char *fname, char *results_folder ) { if(fname == NULL) return; @@ -222,13 +221,17 @@ void GlobalDefaultsForBkgModel::SetGoptDefaults ( char *fname ) if(isJson){ GoptDefaultsFromJson(fname); } else{ - printf("Abort: %s not a json file", fname); exit(1); - } - region_param_start.BadIdeaComputeDerivedInput(); + DumpExcitingParameters("default"); + region_param_start.BadIdeaComputeDerivedInput(); + // emphasis_vector.txt file used in gopt to change the default gopt params + //bool modified = ReadEmphasisVectorFromFile (results_folder); //GeneticOptimizer run - load its vector + bool modified = ReadEmphasisVectorFromJson (results_folder); //GeneticOptimizer run - load its vector + if (modified) + DumpExcitingParameters("modified"); } void GlobalDefaultsForBkgModel::DumpExcitingParameters(const char *fun_string) @@ -237,7 +240,7 @@ void GlobalDefaultsForBkgModel::DumpExcitingParameters(const char *fun_string) printf ( "%s parameters used: \n",fun_string ); region_param_start.DumpPoorlyStructuredText(); - data_control.DumpPoorlyStructuredText(); + data_control.emphasis_params.DumpPoorlyStructuredText(); fitter_defaults.DumpPoorlyStructuredText(); printf ( "\n" ); @@ -275,7 +278,7 @@ void GlobalDefaultsForBkgModel::SetOpts(OptArgs &opts, Json::Value& json_params) char *tmp_config_file = NULL; tmp_config_file = GetIonConfigFile (filename.c_str()); - SetGoptDefaults(tmp_config_file); + SetGoptDefaults(tmp_config_file,(char*)(results_folder.c_str())); if (tmp_config_file) free(tmp_config_file); } @@ -295,21 +298,20 @@ void GlobalDefaultsForBkgModel::SetOpts(OptArgs &opts, Json::Value& json_params) tmp_config_file = GetIonConfigFile (filename.c_str()); } - SetGoptDefaults(tmp_config_file); + SetGoptDefaults(tmp_config_file,(char*)(results_folder.c_str())); if (tmp_config_file) free(tmp_config_file); - } else { - SetGoptDefaults ((char*)(gopt.c_str())); //parameter file provided cmd-line - + SetGoptDefaults ((char*)(gopt.c_str()),(char*)(results_folder.c_str())); //parameter file provided cmd-line + //ReadEmphasisVectorFromFile ((char*)(results_folder.c_str())); //GeneticOptimizer run - load its vector } } signal_process_control.SetOpts(opts, json_params); bool dont_emphasize_by_compression = RetrieveParameterBool(opts, json_params, '-', "bkg-dont-emphasize-by-compression", false); - data_control.point_emphasis_by_compression = (!dont_emphasize_by_compression); + data_control.emphasis_params.point_emphasis_by_compression = (!dont_emphasize_by_compression); // from SetupXtalkParametersForBkgModel string trace_xtalk_name = RetrieveParameterString(opts, json_params, '-', "xtalk", "disable"); @@ -380,3 +382,199 @@ void GlobalDefaultsForBkgModel::SetOpts(OptArgs &opts, Json::Value& json_params) // nothing: no barcodes, don't do anything } } + + +// This function is used during GeneticOptimizer runs in which case the above SetGoptDefaults is disabled + +bool GlobalDefaultsForBkgModel::ReadEmphasisVectorFromJson ( char *experimentName ) +{ + char fname[512]; + sprintf ( fname,"%s/gopt.delta.json", experimentName ); + + struct stat fstatus; + int status = stat ( fname,&fstatus ); + bool modified = false; + + if ( status == 0 ) // file exists + { + Json::Value all_params; + std::ifstream in(fname, std::ios::in); + + if (!in.good()) { + printf("Opening gopt delta file %s unsuccessful. Aborting\n", fname); + exit(1); + } else { + modified = true; + printf ( "loading emphasis vector parameters from %s\n",fname ); + } + in >> all_params; + in.close(); + + // map param to index + const static std::map string_to_case{ + {"km_const[0]",1}, + {"km_const[1]",2}, + {"km_const[2]",3}, + {"km_const[3]",4}, + {"krate[0]",6}, + {"krate[1]",7}, + {"krate[2]",8}, + {"krate[3]",9}, + {"d_coeff[0]",11}, + {"d_coeff[1]",12}, + {"d_coeff[2]",13}, + {"d_coeff[3]",14}, + {"sigma_mult[0]",16}, + {"sigma_mult[1]",17}, + {"sigma_mult[2]",18}, + {"sigma_mult[3]",19}, + {"t_mid_nuc_delay[0]",21}, + {"t_mid_nuc_delay[1]",22}, + {"t_mid_nuc_delay[2]",23}, + {"t_mid_nuc_delay[3]",24}, + {"sens",25}, + {"tau_R_m",26}, + {"tau_R_o",27}, + {"emphasis[0]",28}, + {"emphasis[1]",29}, + {"emphasis[2]",30}, + {"emphasis[3]",31}, + {"emphasis[4]",32}, + {"emphasis[5]",33}, + {"emphasis[6]",34}, + {"emphasis[7]",35}, + {"emp_amplitude",36}, + {"emp_width",37}, + {"clonal_call_scale[0]",38}, + {"clonal_call_scale[1]",39}, + {"clonal_call_scale[2]",40}, + {"clonal_call_scale[3]",41}, + {"clonal_call_scale[4]",42}, + {"min_tauB",43}, + {"max_tauB",44}, + {"mid_tauB",45}, + {"tauB_smooth_range",46} + }; + + // Loop over each element in the object. + for(auto iter = all_params.begin(); iter != all_params.end(); ++iter) + { + // Make sure to get the value as const reference otherwise you will end up copying + // the whole JSON value recursively which can be expensive if it is a nested object. + //const Json::Value &str = iter->first; + //const Json::Value &v = iter->second; + + // Perform actions here to process each string and value in the JSON object... + string key = iter.key().asString(); + cout << "ReadEmphasisVectorFromJson... " << key << ":" << all_params[key] << endl; + float v = all_params[key].asFloat(); + int iCase = string_to_case.count(key) ? string_to_case.at(key) : 0; + set_GoptParameter_byIndex(iCase,v); + } + } + return modified; +} + + +void GlobalDefaultsForBkgModel::set_GoptParameter_byIndex(int iCase, float v) +{ + // note case 0,5,10,15,20 are not handled + switch(iCase) { + case 1: region_param_start.kmax_default[0] *= v; break; + case 2: region_param_start.kmax_default[1] *= v; break; + case 3: region_param_start.kmax_default[2] *= v; break; + case 4: region_param_start.kmax_default[3] *= v; break; + case 6: region_param_start.krate_default[0] *= v; break; + case 7: region_param_start.krate_default[1] *= v; break; + case 8: region_param_start.krate_default[2] *= v; break; + case 9: region_param_start.krate_default[3] *= v; break; + case 11: region_param_start.d_default[0] *= v; break; + case 12: region_param_start.d_default[1] *= v; break; + case 13: region_param_start.d_default[2] *= v; break; + case 14: region_param_start.d_default[3] *= v; break; + case 16: region_param_start.sigma_mult_default[0] *= v; break; + case 17: region_param_start.sigma_mult_default[1] *= v; break; + case 18: region_param_start.sigma_mult_default[2] *= v; break; + case 19: region_param_start.sigma_mult_default[3] *= v; break; + case 21: region_param_start.t_mid_nuc_delay_default[0] *= v; break; + case 22: region_param_start.t_mid_nuc_delay_default[1] *= v; break; + case 23: region_param_start.t_mid_nuc_delay_default[2] *= v; break; + case 24: region_param_start.t_mid_nuc_delay_default[3] *= v; break; + case 25: region_param_start.sens_default *= v; break; + case 26: region_param_start.tau_R_m_default *= v; break; + case 27: region_param_start.tau_R_o_default *= v; break; + case 28: data_control.emphasis_params.emp[0] *= v; break; + case 29: data_control.emphasis_params.emp[1] *= v; break; + case 30: data_control.emphasis_params.emp[2] *= v; break; + case 31: data_control.emphasis_params.emp[3] *= v; break; + case 32: data_control.emphasis_params.emp[4] *= v; break; + case 33: data_control.emphasis_params.emp[5] *= v; break; + case 34: data_control.emphasis_params.emp[6] *= v; break; + case 35: data_control.emphasis_params.emp[7] *= v; break; + case 36: data_control.emphasis_params.emphasis_ampl_default *= v; break; + case 37: data_control.emphasis_params.emphasis_width_default *= v; break; + case 38: fitter_defaults.clonal_call_scale[0] *= v; break; + case 39: fitter_defaults.clonal_call_scale[1] *= v; break; + case 40: fitter_defaults.clonal_call_scale[2] *= v; break; + case 41: fitter_defaults.clonal_call_scale[3] *= v; break; + case 42: fitter_defaults.clonal_call_scale[4] *= v; break; + case 43: region_param_start.tau_E_default *= v; break; + case 44: region_param_start.min_tauB_default *= v; break; + case 45: region_param_start.max_tauB_default *= v; break; + case 46: region_param_start.tauB_smooth_range_default *= v; break; + case 0: + default: + cout << "ReadEmphasisVectorFromJson...cannot handle parameter index " << iCase << ":" << v << endl; + exit(1); + } +} + + +// note: ReadEmphasisVectorFromFile() could be removed and replaced by ReadEmphasisVectorFromJson() +// keep it for now in case we have problem with ReadEmphasisVectorFromJson() +// note case 0,5,10,15,20 are not handled in ReadEmphasisVectorFromJson() +#define MAX_LINE_LEN 2048 +#define MAX_DATA_PTS 80 +void GlobalDefaultsForBkgModel::ReadEmphasisVectorFromFile ( char *experimentName ) +{ + char fname[512]; + FILE *evect_file; + char *line = new char[MAX_LINE_LEN]; + float read_data[MAX_DATA_PTS]; + int nChar = MAX_LINE_LEN; + + struct stat fstatus; + sprintf ( fname,"%s/emphasis_vector.txt", experimentName ); + int status = stat ( fname,&fstatus ); + //bool modified = false; + if ( status == 0 ) // file exists + { + //modified = true; + printf ( "loading emphasis vector parameters from %s\n",fname ); + evect_file=fopen ( fname,"rt" ); + // first line contains the number of points + int bytes_read = getline ( &line, ( size_t * ) &nChar,evect_file ); + if ( bytes_read > 0 ) + { + int evect_size; + sscanf ( line,"%d",&evect_size ); + printf ("nps=%d",evect_size); + for (int i=0; ( i < evect_size ) && ( i < MAX_DATA_PTS );i++ ) + { + bytes_read = getline ( &line, ( size_t * ) &nChar,evect_file ); + sscanf ( line,"%f",&read_data[i] ); + printf ("\t%f",read_data[i]); + } + printf ("\n"); + for (int i=0; ( i < evect_size ) && ( i < MAX_DATA_PTS );i++ ) + { + if (read_data[i] != 1) { + printf ("Setting parameter %d *= %f\n",i,read_data[i]); + set_GoptParameter_byIndex(i, read_data[i]); + } + } + } + } + delete [] line; + //return modified; +} diff --git a/Analysis/BkgModel/GlobalDefaultsForBkgModel.h b/Analysis/BkgModel/GlobalDefaultsForBkgModel.h index 4277809a..8ba6ae21 100644 --- a/Analysis/BkgModel/GlobalDefaultsForBkgModel.h +++ b/Analysis/BkgModel/GlobalDefaultsForBkgModel.h @@ -167,17 +167,19 @@ class GlobalDefaultsForBkgModel void FixRdrInFirst20Flows(bool fixed_RatioDrift) { signal_process_control.no_RatioDrift_fit_first_20_flows = fixed_RatioDrift; } void SetUse_alternative_etbR_equation(bool if_use_alternative_etbR_equation) { signal_process_control.use_alternative_etbR_equation = if_use_alternative_etbR_equation; } - void SetFittingTauE(bool fit_taue) { signal_process_control.fitting_taue = fit_taue; } + void SetFittingTauE(bool fit_taue) { signal_process_control.fitting_taue = fit_taue; } void SetHydrogenModel( int model ) { signal_process_control.hydrogenModelType = model; } void SetFitGaussNewton(bool _fit_gauss_newton){signal_process_control.fit_gauss_newton = _fit_gauss_newton;}; - void SetEmphasizeByCompression(bool _emp_by_comp){data_control.point_emphasis_by_compression = _emp_by_comp;}; + void SetEmphasizeByCompression(bool _emp_by_comp){data_control.emphasis_params.point_emphasis_by_compression = _emp_by_comp;}; void ReadXtalk(char *name); void SetChipType(const char *name); // i/o from files for parameters - void SetGoptDefaults(char *gopt); - void ReadEmphasisVectorFromFile(char *experimentName); + void SetGoptDefaults(char *gopt, char *results_folder); + void ReadEmphasisVectorFromFile(char *experimentName); + bool ReadEmphasisVectorFromJson(char *experimentName); + void set_GoptParameter_byIndex(int iCase, float v); void DumpExcitingParameters(const char *fun_string); void GoptDefaultsFromJson(char *fname); void GoptDefaultsFromPoorlyStructuredFile(char *fname); diff --git a/Analysis/BkgModel/LocalTrace/BkgTrace.cpp b/Analysis/BkgModel/LocalTrace/BkgTrace.cpp index da6713f7..05c6b8f9 100644 --- a/Analysis/BkgModel/LocalTrace/BkgTrace.cpp +++ b/Analysis/BkgModel/LocalTrace/BkgTrace.cpp @@ -9,6 +9,8 @@ using namespace std; //#define DEBUG_BKTRC 1 +#define WITH_SUBTRACT 1 + double GenAllBtrc_time=0; double ReZero_time=0; @@ -1014,16 +1016,22 @@ void BkgTrace::LoadImgWRezeroOffset(const RawImage *raw, int16_t *out[VEC8_SIZE] } //#define BEADTRACE_CHKBOTH_DBG 1 -void BkgTrace::GenerateAllBeadTrace (Region *region, BeadTracker &my_beads, Image *img, int iFlowBuffer, int flow_block_size) +void BkgTrace::GenerateAllBeadTrace (Region *region, BeadTracker &my_beads, Image *img, int iFlowBuffer, + int flow_block_size, float t_start, float t_end) { #ifndef BEADTRACE_CHKBOTH_DBG // Timer t; // t.restart(); if(/*0 && */(((region->w % VEC8_SIZE) == 0) && - ((img->GetImage()->cols % VEC8_SIZE) == 0))) // check that pointers are alligned too. - GenerateAllBeadTrace_vec(region,my_beads,img,iFlowBuffer,fg_buffers, flow_block_size); - else + ((img->GetImage()->cols % VEC8_SIZE) == 0))) {// check that pointers are alligned too. + GenerateAllBeadTrace_vec(region,my_beads,img,iFlowBuffer,fg_buffers, flow_block_size, t_start, t_end); +#ifndef WITH_SUBTRACT + RezeroBeads (t_start, t_end, iFlowBuffer,flow_block_size); +#endif + }else{ GenerateAllBeadTrace_nonvec(region,my_beads,img,iFlowBuffer,fg_buffers, flow_block_size); + RezeroBeads (t_start, t_end, iFlowBuffer,flow_block_size); + } // cout << "Generate Bead Trace: " << t.elapsed() << "s" << endl; @@ -1198,77 +1206,108 @@ void BkgTrace::GenerateAllBeadTraceAnRezero (Region *region, BeadTracker &my_bea void BkgTrace::GenerateAllBeadTrace_vec (Region *region, BeadTracker &my_beads, - Image *img, int iFlowBuffer, FG_BUFFER_TYPE *fgb, int flow_block_size) + Image *img, int iFlowBuffer, FG_BUFFER_TYPE *fgb, int flow_block_size, + float t_start, float t_end) { // these are used by both the background and live-bead - int k; + uint k; int nbdx=0; int npts=time_cp->npts(); - FG_BUFFER_TYPE *fgPtr; - float localT0; + FG_BUFFER_TYPE *fgPtr __attribute__ ((aligned(16))); + int16_t *sptr __attribute__ ((aligned(16))); + int16_t *imgPtr __attribute__ ((aligned(16))); const RawImage *raw = img->GetImage(); + float localT0; int x,y; - int t0ShiftWhole; - float multT; - float t0ShiftFrac; + int t0ShiftWhole=0; + float t0ShiftFrac=0; int my_frame = 0,compFrm,curFrms,curCompFrms; - v8f_u prev; - v8f_u next; - v8f_u tmpAdder; - v8f_u mult; - v8f_u curCompFrmsV; + +#define MY_VEC_SIZE 8 +#define MY_VECF v8f_u +#define MY_VECS v8s_u + + + MY_VECF prev; + MY_VECF next; + MY_VECF dummy={}; + MY_VECF tmpAdder; +#ifdef WITH_SUBTRACT + MY_VECF tmpTrace[npts]; + MY_VECS tmpTraceS[npts]; +#endif + int frameStride=raw->rows*raw->cols; int interf,lastInterf=-1; - int16_t *sptr, *imgPtr; - int storeIdx[VEC8_SIZE]; + int storeIdx[MY_VEC_SIZE]; Timer tmr; +#ifdef WITH_SUBTRACT + int start_pt=0; + int end_pt=0; + float dc_cnt; + float overhang_start=0.0f; + float overhang_end=0.0f; + int overhang_start_pt=1; + int overhang_end_pt=1; + + ComputeDcOffset_params(t_start, t_end, start_pt, end_pt, dc_cnt, + overhang_start, overhang_end); + + if(start_pt > 0) + overhang_start_pt = start_pt-1; + else + overhang_start_pt = 0; + + if(end_pt > 0 && end_pt < npts) + overhang_end_pt = end_pt; + else + overhang_end_pt=0; + MY_VECF ohsv; + MY_VECF ohev; + ohsv.V = dummy.V + overhang_start; + ohev.V = dummy.V + overhang_end; +#endif + fgPtr = &fgb[npts * flow_block_size*nbdx+npts*iFlowBuffer]; for (y = 0; y < region->h; y++) { imgPtr = &raw->image[(y+region->row)*raw->cols+region->col]; - for (x = 0;x < region->w && nbdx < numLBeads; x+=VEC8_SIZE,imgPtr+=VEC8_SIZE) + for (x = 0;x < region->w && nbdx < numLBeads; x+=MY_VEC_SIZE,imgPtr+=MY_VEC_SIZE) { -#ifdef BEADTRACE_DBG - int doDebug=0; - if(my_beads.params_nn[nbdx].x == 1 && my_beads.params_nn[nbdx].y == 0 && - region->row == DBG_ROW && region->col == DBG_COL) - { - doDebug=1; - } -#endif int incr=0; int nbdxCopy=nbdx; float localT0Cnt=0.0f; localT0=0.0f; - for(k=0;kw+(x+k)]; //BUG!! t0_map is a 2D map containing all beads not only live beads. - localT0Cnt += 1.0f; - storeIdx[k]=incr++; - nbdx++; - } - } + for(k=0;kw+(x+k)]; //BUG!! t0_map is a 2D map containing all beads not only live beads. + localT0Cnt += 1.0f; + storeIdx[k]=incr++; + nbdx++; + }else{ +// printf("%d/%d: %f\n",y,x+k,t0_map[y*region->w+(x+k)]); + } + } if(nbdxCopy == nbdx) continue; // there are no live beads in this chunk localT0 /= localT0Cnt; - //allow for negative t0shift, faster traces + //allow for negative t0shift, faster traces if(localT0 < 0-(raw->uncompFrames-2)) localT0 = 0-(raw->uncompFrames-2); if(localT0 > (raw->uncompFrames-2)) localT0 = (raw->uncompFrames-2); - //by using floor() instead of (int) here - //we now can allow for negative t0Shifts + //by using floor() instead of (int) here + //we now can allow for negative t0Shifts t0ShiftWhole=floor(localT0); t0ShiftFrac = localT0 - (float)t0ShiftWhole; @@ -1277,85 +1316,122 @@ void BkgTrace::GenerateAllBeadTrace_vec (Region *region, BeadTracker &my_beads, my_frame = raw->interpolatedFrames[StartAtFrame]-1; compFrm = 0; - tmpAdder.V=LD_VEC8F(0.0f); - prev.V=LD_VEC8F(0.0f); + tmpAdder.V=0.0f+dummy.V; + prev.V=0.0f+dummy.V; curFrms=0; curCompFrms=time_cp->frames_per_point[compFrm]; - interf= raw->interpolatedFrames[my_frame]; - sptr = &imgPtr[interf*frameStride]; + interf= raw->interpolatedFrames[my_frame]; + sptr = &imgPtr[interf*frameStride]; - LD_VEC8S_CVT_VEC8F(sptr,next); + LD_VS_VF(sptr, next); - while ((my_frame < raw->uncompFrames) && (compFrm < npts)) - { - interf= raw->interpolatedFrames[my_frame]; - - if(interf != lastInterf) - { - sptr = &imgPtr[interf*frameStride]; - prev.V = next.V; - LD_VEC8S_CVT_VEC8F(sptr,next); - } - - // interpolate - multT=raw->interpolatedMult[my_frame] - (t0ShiftFrac/raw->interpolatedDiv[my_frame]); - mult.V = LD_VEC8F(multT); - tmpAdder.V += ( (prev.V)-(next.V) ) * (mult.V) + (next.V); -#ifdef BEADTRACE_DBG - if(doDebug) - printf(" tmpAddr(%f) += (%f-%f) * %f + %f\n",tmpAdder.A[0],prev.A[0],next.A[0],mult.A[0],next.A[0]); -#endif - if(++curFrms >= curCompFrms) - { - curCompFrmsV.V = LD_VEC8F((float)curCompFrms); - tmpAdder.V /= curCompFrmsV.V; - // now, turn it back into short int's - v8s_u svalV; - CVT_VEC8F_VEC8S(svalV,tmpAdder); - - for(k=0;kuncompFrames) && (compFrm < npts)) { + interf= raw->interpolatedFrames[my_frame]; + + if(interf != lastInterf) { + sptr = &imgPtr[interf*frameStride]; + prev.V = next.V; + LD_VS_VF(sptr, next); + } + + // interpolate + MY_VECF mult; + float tmpMult = (raw->interpolatedMult[my_frame] + - (t0ShiftFrac/raw->interpolatedDiv[my_frame])); + mult.V = dummy.V + tmpMult; + //BC_VEC(mult,&tmpMult); + tmpAdder.V += ((prev.V)-(next.V) ) * (mult.V) + (next.V); + if(++curFrms >= curCompFrms) { + tmpAdder.V = tmpAdder.V / ((float )curCompFrms); + +#ifdef WITH_SUBTRACT + tmpTrace[compFrm].V = tmpAdder.V; +#else + MY_VECS svalV; + CVT_VF_VS(svalV,tmpAdder); + + for(k=0;k= 0) fgPtr[storeIdx[k]*npts*flow_block_size+compFrm] = svalV.A[k]; -// fgPtr[storeIdx[k]*npts*flow_block_size+compFrm] = tmpAdder.A[k]; } -#ifdef BEADTRACE_DBG - if(doDebug) - printf("V Writing %d\n",svalV.A[0]); #endif - compFrm++; - curCompFrms = time_cp->frames_per_point[compFrm]; - curFrms=0; - tmpAdder.V = LD_VEC8F(0.0f); - } - //reuse current my_frame while not compensated for negative t0 shift - if(t0ShiftWhole < 0) - t0ShiftWhole++; - else - my_frame++; + compFrm++; + curCompFrms = time_cp->frames_per_point[compFrm]; + curFrms=0; + tmpAdder.V = dummy.V + 0.0f; + } + //reuse current my_frame while not compensated for negative t0 shift + if(t0ShiftWhole < 0) + t0ShiftWhole++; + else + my_frame++; - } + } if(compFrm > 0 && compFrm < npts) { for(;compFrm < npts;compFrm++) { - for(k=0;k= 0) fgPtr[storeIdx[k]*npts*flow_block_size + compFrm] = fgPtr[storeIdx[k]*npts*flow_block_size + compFrm-1]; } + +#endif } + } + +#ifdef WITH_SUBTRACT + // do the trace zeroing... + { + MY_VECF dc_zero; + dc_zero.V=dummy.V + 0.0f; + + for (int pt = start_pt; pt < end_pt; pt++){ + dc_zero.V += (tmpTrace[pt].V); + } + + // add end interpolation parts + dc_zero.V += ohsv.V*(tmpTrace[overhang_start_pt].V); + dc_zero.V += ohev.V*(tmpTrace[overhang_end_pt].V); + + // make it into an average + dc_zero.V /= dummy.V + dc_cnt; + + // now, subtract the dc offset from all the points + for (int pt = 0;pt < npts;pt++){ // over real data + tmpTrace[pt].V -= dc_zero.V; + CVT_VF_VS(tmpTraceS[pt],tmpTrace[pt]); + } } - for(k=0;k= 0){ + int stidx=storeIdx[k] * npts * flow_block_size; + + for (int frm=0; frm < compFrm; frm++) { + fgPtr[stidx + frm] = tmpTraceS[frm].V[k]; +// fgPtr[stidx + frm] = tmpTrace[frm].V[k]; + } + } + } +#endif + + for(k=0;k= 0) fgPtr += npts * flow_block_size; // advance one bead } - } + } + } /* diff --git a/Analysis/BkgModel/LocalTrace/BkgTrace.h b/Analysis/BkgModel/LocalTrace/BkgTrace.h index f039395d..a82cc7f1 100644 --- a/Analysis/BkgModel/LocalTrace/BkgTrace.h +++ b/Analysis/BkgModel/LocalTrace/BkgTrace.h @@ -47,9 +47,9 @@ class BkgTrace{ void RezeroOneBead(float t_start, float t_end, int fnum, int ibd, int flow_block_size); void RezeroBeadsAllFlows (float t_start, float t_end); void RezeroUncompressedTraceV(void *Ptr, float t_start, float t_end); - void GenerateAllBeadTrace(Region *region, BeadTracker &my_beads, Image *img, int iFlowBuffer, int flow_block_size); + void GenerateAllBeadTrace(Region *region, BeadTracker &my_beads, Image *img, int iFlowBuffer, int flow_block_size,float t_start, float t_end); void GenerateAllBeadTrace_nonvec(Region *region, BeadTracker &my_beads, Image *img, int iFlowBuffer, FG_BUFFER_TYPE *fgb, int flow_block_size); - void GenerateAllBeadTrace_vec(Region *region, BeadTracker &my_beads, Image *img, int iFlowBuffer, FG_BUFFER_TYPE *fgb, int flow_block_size); + void GenerateAllBeadTrace_vec(Region *region, BeadTracker &my_beads, Image *img, int iFlowBuffer, FG_BUFFER_TYPE *fgb, int flow_block_size, float t_start, float t_end); void GenerateAllBeadTraceAnRezero(Region *region, BeadTracker &my_beads, Image *img, int iFlowBuffer, int flow_block_size, float t_start, float t_end); #define BKTRC_VEC_SIZE 8 diff --git a/Analysis/BkgModel/MathModel/MathOptim.cpp b/Analysis/BkgModel/MathModel/MathOptim.cpp index b3bf2674..9a402ff4 100644 --- a/Analysis/BkgModel/MathModel/MathOptim.cpp +++ b/Analysis/BkgModel/MathModel/MathOptim.cpp @@ -79,6 +79,8 @@ float ErfApprox ( float x ) return ( ret * sign ); } + +#if 0 float ExpApprox ( float x ) { int left, right; @@ -112,7 +114,7 @@ float ExpApprox ( float x ) return ( ret ); } - +#endif diff --git a/Analysis/BkgModel/MathModel/MathOptim.h b/Analysis/BkgModel/MathModel/MathOptim.h index 20cfa566..35d5986d 100644 --- a/Analysis/BkgModel/MathModel/MathOptim.h +++ b/Analysis/BkgModel/MathModel/MathOptim.h @@ -136,6 +136,12 @@ class MixtureMemo float ErfApprox ( float x ); float Expm2Approx ( float x ); -float ExpApprox ( float x ); +inline float ExpApprox ( float x ) +{ + x = 1.0 + x / 256.0; + x *= x; x *= x; x *= x; x *= x; + x *= x; x *= x; x *= x; x *= x; + return x; +} #endif // MATHOPTIM_H diff --git a/Analysis/BkgModel/RegionalizedData.cpp b/Analysis/BkgModel/RegionalizedData.cpp index e1827bb8..dd2497fa 100644 --- a/Analysis/BkgModel/RegionalizedData.cpp +++ b/Analysis/BkgModel/RegionalizedData.cpp @@ -118,7 +118,7 @@ void RegionalizedData::SetTimeAndEmphasis (GlobalDefaultsForBkgModel &global_def if (global_defaults.signal_process_control.recompress_tail_raw_trace) // Generate emphasis vector object for standard time compression - SetUpEmphasisForStandardCompression(global_defaults); + std_time_comp_emphasis.SetUpEmphasis(global_defaults.data_control, time_c); } else // check the points that we need @@ -129,36 +129,42 @@ void RegionalizedData::SetTimeAndEmphasis (GlobalDefaultsForBkgModel &global_def // assuming that our t_mid_nuc estimation is decent // see what the emphasis functions needed for "detailed" results are // and assume the "coarse" blank emphasis function will work out fine. - trial_emphasis.SetDefaultValues (global_defaults.data_control.emp,global_defaults.data_control.emphasis_ampl_default, global_defaults.data_control.emphasis_width_default); - trial_emphasis.SetupEmphasisTiming (time_c.npts(), &time_c.frames_per_point[0],&time_c.frameNumber[0]); - trial_emphasis.point_emphasis_by_compression = global_defaults.data_control.point_emphasis_by_compression; - // trial_emphasis.BuildCurrentEmphasisTable (t_mid_nuc_start, FINEXEMPHASIS); + trial_emphasis.SetUpEmphasis(global_defaults.data_control,time_c); trial_emphasis.BuildCurrentEmphasisTable (t0_offset, FINEXEMPHASIS); - //time_c.npts(trial_emphasis.ReportUnusedPoints (CENSOR_THRESHOLD, MIN_CENSOR)); // threshold the points for the number actually needed by emphasis time_c.SetStandardFrames(trial_emphasis.ReportUnusedPoints (CENSOR_THRESHOLD, MIN_CENSOR)); time_c.UseStandardCompression(); - - // don't bother monitoring this now - //printf ("Saved: %f = %d of %d\n", (1.0*time_c.npts) / (1.0*old_pts), time_c.npts, old_pts); - // now give the emphasis data structure (and everything else) using the "used" number of points } - emphasis_data.SetDefaultValues (global_defaults.data_control.emp,global_defaults.data_control.emphasis_ampl_default, global_defaults.data_control.emphasis_width_default); - emphasis_data.SetupEmphasisTiming (time_c.npts(), &time_c.frames_per_point[0],&time_c.frameNumber[0]); - emphasis_data.point_emphasis_by_compression = global_defaults.data_control.point_emphasis_by_compression; - // emphasis_data.BuildCurrentEmphasisTable (t_mid_nuc_start, FINEXEMPHASIS); + emphasis_data.SetUpEmphasis(global_defaults.data_control, time_c); emphasis_data.BuildCurrentEmphasisTable (t0_offset, FINEXEMPHASIS); -#if 0 - static int doneOnce=0; - if(!doneOnce){ - doneOnce=1; - emphasis_data.SaveEmphasisVector(); - } -#endif } +void RegionalizedData::SetCrudeEmphasisVectors() +{ + // head off bad behaviour if this region was skipped during processing of previous block of flows. + // not clear this is such a great way to solve this problem. + if(my_beads.numLBeads != 0) + emphasis_data.BuildCurrentEmphasisTable (GetTypicalMidNucTime (& (my_regions.rp.nuc_shape)), CRUDEXEMPHASIS); // why is this not per nuc? +} + +void RegionalizedData::SetFineEmphasisVectors() +{ + emphasis_data.BuildCurrentEmphasisTable (GetTypicalMidNucTime (& (my_regions.rp.nuc_shape)), FINEXEMPHASIS); +} + +void RegionalizedData::GenerateFineEmphasisForStdTimeCompression() +{ + std_time_comp_emphasis.BuildCurrentEmphasisTable ( + GetTypicalMidNucTime (& (my_regions.rp.nuc_shape)), + FINEXEMPHASIS); +} + +void RegionalizedData::SetUpEmphasisForStandardCompression(GlobalDefaultsForBkgModel &global_defaults) +{ +} + void RegionalizedData::SetupTimeAndBuffers ( GlobalDefaultsForBkgModel &global_defaults,float sigma_guess, float t_mid_nuc_guess, @@ -277,31 +283,17 @@ void RegionalizedData::UpdateTracesFromImage (Image *img, FlowBufferInfo &my_flo float t_offset_beads = my_regions.rp.nuc_shape.sigma; #if 1 + // populate bead traces from image file and // time-shift traces for uniform start times; compress traces to flows buffer - my_trace.GenerateAllBeadTrace (region,my_beads,img, my_flow.flowBufferWritePos, flow_block_size); + my_trace.GenerateAllBeadTrace (region,my_beads,img, my_flow.flowBufferWritePos, flow_block_size,time_c.time_start, t_mid_nuc-t_offset_beads); // subtract mean signal in time before flow starts from traces in flows buffer - my_trace.RezeroBeads (time_c.time_start, t_mid_nuc-t_offset_beads, - my_flow.flowBufferWritePos, flow_block_size); - -#if 0 - if(flow > 19){ - std::cout << "Sampled for Flow " << flow << std::endl; - int nPts = my_trace.time_cp->npts(); - FG_BUFFER_TYPE *fgPtr = &my_trace.fg_buffers[my_flow.flowBufferWritePos*nPts]; - for(int n = 0; n < my_beads.numLBeads; n++){ - if(my_beads.Sampled(n)){ - std::cout << "regId," << 0 <<",x,"<< my_beads.params_nn[n].x << ",y,"<< my_beads.params_nn[n].y; - for (int pt = 0;pt < nPts;pt++){ // over real data - std::cout << "," << fgPtr[pt]; - } - std::cout << std::endl; - } - fgPtr += my_trace.npts * flow_block_size; - } - } -#endif +// now the RezeroBeads is done in GereateAllBeadTrace +// my_trace.RezeroBeads (time_c.time_start, t_mid_nuc-t_offset_beads, +// my_flow.flowBufferWritePos, flow_block_size); + + #else //Do it all at once.. generate bead trace and rezero like it is done in the new GPU pipeline my_trace.GenerateAllBeadTraceAnRezero(region,my_beads,img, my_flow.flowBufferWritePos, flow_block_size, @@ -345,7 +337,7 @@ void RegionalizedData::PickRepresentativeHighQualityWells (float copy_stringency my_beads.my_mean_copy_count = my_beads.KeyNormalizeSampledReads ( true, flow_block_size ); float stringent_filter = my_beads.my_mean_copy_count; int num_sampled = my_beads.NumberSampled(); // weird that I need to know this - if (copy_stringency>0.0f){ + if (copy_stringency>0.0f){ // make filter >stringent< as though the average bead were a certain copy count // this replicates a 'bug' that led to stringent filtering and slight performance changes @@ -354,7 +346,7 @@ void RegionalizedData::PickRepresentativeHighQualityWells (float copy_stringency // but if we wet the filter to be too stringent, we can lose all beads in a region // set a minimum number of beads to succeed with and we can move on - // technically, I'm setting the minimum >rank< here + // technically, I'm setting the minimum >rank< here float min_percentage = min_beads; min_percentage /= num_sampled; min_percentage = 1.0f-min_percentage; @@ -567,25 +559,6 @@ RegionalizedData::~RegionalizedData() } -void RegionalizedData::SetCrudeEmphasisVectors() -{ - // head off bad behaviour if this region was skipped during processing of previous block of flows. - // not clear this is such a great way to solve this problem. - if(my_beads.numLBeads != 0) - emphasis_data.BuildCurrentEmphasisTable (GetTypicalMidNucTime (& (my_regions.rp.nuc_shape)), CRUDEXEMPHASIS); // why is this not per nuc? -} - -void RegionalizedData::SetFineEmphasisVectors() -{ - emphasis_data.BuildCurrentEmphasisTable (GetTypicalMidNucTime (& (my_regions.rp.nuc_shape)), FINEXEMPHASIS); -} - -void RegionalizedData::GenerateFineEmphasisForStdTimeCompression() -{ - std_time_comp_emphasis.BuildCurrentEmphasisTable ( - GetTypicalMidNucTime (& (my_regions.rp.nuc_shape)), - FINEXEMPHASIS); -} @@ -599,20 +572,6 @@ void RegionalizedData::DumpEmptyTrace (FILE *my_fp, int flow_block_size) } } -void RegionalizedData::SetUpEmphasisForStandardCompression(GlobalDefaultsForBkgModel &global_defaults) -{ - std_time_comp_emphasis.SetDefaultValues ( - global_defaults.data_control.emp, - global_defaults.data_control.emphasis_ampl_default, - global_defaults.data_control.emphasis_width_default); - std_time_comp_emphasis.SetupEmphasisTiming ( - time_c.GetStdFrames(), - &((time_c.GetStdFramesPerPoint())[0]), - &((time_c.GetStdFrameNumber())[0])); - std_time_comp_emphasis.point_emphasis_by_compression = - global_defaults.data_control.point_emphasis_by_compression; - -} bool RegionalizedData::isRegionCenter(int ibd) diff --git a/Analysis/BkgModel/SignalProcessingMasterFitter.cpp b/Analysis/BkgModel/SignalProcessingMasterFitter.cpp index e76eb464..49d516dd 100644 --- a/Analysis/BkgModel/SignalProcessingMasterFitter.cpp +++ b/Analysis/BkgModel/SignalProcessingMasterFitter.cpp @@ -10,7 +10,7 @@ #include "SignalProcessingMasterFitter.h" #include "RawWells.h" #include "MathOptim.h" -#include "mixed.h" +#include "ClonalFilter/mixed.h" #include "BkgDataPointers.h" #include "DNTPRiseModel.h" #include "DiffEqModel.h" @@ -424,6 +424,8 @@ void SignalProcessingMasterFitter::BkgModelInit ( bool debug_trace_enable,float if ( ( !NeverProcessRegion() ) && debug_trace_enable ) my_debug.DebugFileOpen ( global_state.dirName, region_data->region ); + IF_OPTIMIZER_DEBUG( inception_state, debugSaver.DebugFileOpen( global_state.dirName ) ); + // Rest of initialization delayed until SetupTimeAndBuffers() called. } @@ -594,11 +596,11 @@ void SignalProcessingMasterFitter::FirstPassSampledRegionParamFit( int flow_key, MultiFlowLevMar first_lev_mar_fit( *this, flow_block_size, table ); - first_lev_mar_fit.MultiFlowSpecializedSampledLevMarFitParameters ( 1, 3, first_lev_mar_fit.fit_control.FitWellAmplBuffering, first_lev_mar_fit.fit_control.FitRegionTmidnucPlus, SMALL_LAMBDA , NO_NONCLONAL_PENALTY, flow_key, flow_block_size, flow_block_start ); + first_lev_mar_fit.MultiFlowSpecializedSampledLevMarFitParameters ( 1, 3, first_lev_mar_fit.fit_control.GetFitPacker("FitWellAmplBuffering"), first_lev_mar_fit.fit_control.GetFitPacker("FitRegionTmidnucPlus"), SMALL_LAMBDA , NO_NONCLONAL_PENALTY, flow_key, flow_block_size, flow_block_start ); region_data->my_beads.my_mean_copy_count = region_data->my_beads.KeyNormalizeSampledReads ( true, flow_block_size ); - first_lev_mar_fit.MultiFlowSpecializedSampledLevMarFitParameters ( 1, 1, first_lev_mar_fit.fit_control.FitWellAmplBuffering, first_lev_mar_fit.fit_control.FitRegionTmidnucPlus, SMALL_LAMBDA , NO_NONCLONAL_PENALTY, flow_key, flow_block_size, flow_block_start ); + first_lev_mar_fit.MultiFlowSpecializedSampledLevMarFitParameters ( 1, 1, first_lev_mar_fit.fit_control.GetFitPacker("FitWellAmplBuffering"), first_lev_mar_fit.fit_control.GetFitPacker("FitRegionTmidnucPlus"), SMALL_LAMBDA , NO_NONCLONAL_PENALTY, flow_key, flow_block_size, flow_block_start ); region_data->my_beads.my_mean_copy_count = region_data->my_beads.KeyNormalizeSampledReads ( true, flow_block_size ); - first_lev_mar_fit.MultiFlowSpecializedSampledLevMarFitParameters ( 1,1, first_lev_mar_fit.fit_control.FitWellAmplBuffering, first_lev_mar_fit.fit_control.FitRegionTmidnucPlus,SMALL_LAMBDA , NO_NONCLONAL_PENALTY, flow_key, flow_block_size, flow_block_start ); + first_lev_mar_fit.MultiFlowSpecializedSampledLevMarFitParameters ( 1,1, first_lev_mar_fit.fit_control.GetFitPacker("FitWellAmplBuffering"), first_lev_mar_fit.fit_control.GetFitPacker("FitRegionTmidnucPlus"),SMALL_LAMBDA , NO_NONCLONAL_PENALTY, flow_key, flow_block_size, flow_block_start ); } // first pass fit using all beads @@ -609,11 +611,11 @@ void SignalProcessingMasterFitter::FirstPassRegionParamFit( int flow_key, int fl MultiFlowLevMar first_lev_mar_fit( *this, flow_block_size, table ); - first_lev_mar_fit.MultiFlowSpecializedLevMarFitParameters ( 1, 3, first_lev_mar_fit.fit_control.FitWellAmplBuffering, first_lev_mar_fit.fit_control.FitRegionTmidnucPlus, SMALL_LAMBDA , NO_NONCLONAL_PENALTY, flow_key, flow_block_size, flow_block_start ); + first_lev_mar_fit.MultiFlowSpecializedLevMarFitParameters ( 1, 3, first_lev_mar_fit.fit_control.GetFitPacker("FitWellAmplBuffering"), first_lev_mar_fit.fit_control.GetFitPacker("FitRegionTmidnucPlus"), SMALL_LAMBDA , NO_NONCLONAL_PENALTY, flow_key, flow_block_size, flow_block_start ); region_data->my_beads.my_mean_copy_count = region_data->my_beads.KeyNormalizeReads ( true, false, flow_block_size ); - first_lev_mar_fit.MultiFlowSpecializedLevMarFitParameters ( 1, 1, first_lev_mar_fit.fit_control.FitWellAmplBuffering, first_lev_mar_fit.fit_control.FitRegionTmidnucPlus, SMALL_LAMBDA, NO_NONCLONAL_PENALTY, flow_key, flow_block_size, flow_block_start ); + first_lev_mar_fit.MultiFlowSpecializedLevMarFitParameters ( 1, 1, first_lev_mar_fit.fit_control.GetFitPacker("FitWellAmplBuffering"), first_lev_mar_fit.fit_control.GetFitPacker("FitRegionTmidnucPlus"), SMALL_LAMBDA, NO_NONCLONAL_PENALTY, flow_key, flow_block_size, flow_block_start ); region_data->my_beads.my_mean_copy_count = region_data->my_beads.KeyNormalizeReads ( true, false, flow_block_size ); - first_lev_mar_fit.MultiFlowSpecializedLevMarFitParameters ( 1, 1, first_lev_mar_fit.fit_control.FitWellAmplBuffering, first_lev_mar_fit.fit_control.FitRegionTmidnucPlus, SMALL_LAMBDA , NO_NONCLONAL_PENALTY, flow_key, flow_block_size, flow_block_start ); + first_lev_mar_fit.MultiFlowSpecializedLevMarFitParameters ( 1, 1, first_lev_mar_fit.fit_control.GetFitPacker("FitWellAmplBuffering"), first_lev_mar_fit.fit_control.GetFitPacker("FitRegionTmidnucPlus"), SMALL_LAMBDA , NO_NONCLONAL_PENALTY, flow_key, flow_block_size, flow_block_start ); } @@ -627,8 +629,8 @@ void SignalProcessingMasterFitter::PostKeyFitNoRegionalSampling (MultiFlowLevMar fit_timer.restart(); region_data->RezeroByCurrentTiming( flow_block_size ); // rezeroing?? - - post_key_fit.MultiFlowSpecializedLevMarFitParameters ( NO_ADDITIONAL_WELL_ITERATIONS, STANDARD_POST_KEY_ITERATIONS, post_key_fit.fit_control.FitWellPostKey, post_key_fit.fit_control.FitRegionInit2, LARGER_LAMBDA , NO_NONCLONAL_PENALTY, flow_key, flow_block_size, flow_block_start ); + bool fittaue = global_defaults.signal_process_control.fitting_taue; + post_key_fit.MultiFlowSpecializedLevMarFitParameters ( NO_ADDITIONAL_WELL_ITERATIONS, STANDARD_POST_KEY_ITERATIONS, post_key_fit.fit_control.GetFitPacker("FitWellPostKey"), post_key_fit.fit_control.GetFitPacker(fittaue?"FitRegionInit2TauE":"FitRegionInit2"), LARGER_LAMBDA , NO_NONCLONAL_PENALTY, flow_key, flow_block_size, flow_block_start ); elapsed_time += fit_timer.elapsed(); // classify beads here? @@ -643,9 +645,9 @@ void SignalProcessingMasterFitter::PostKeyFitNoRegionalSampling (MultiFlowLevMar post_key_fit.lm_state.ref_penalty_scale = global_defaults.signal_process_control.barcode_penalty; // big penalty for getting these wrong! post_key_fit.lm_state.kmult_penalty_scale = global_defaults.signal_process_control.kmult_penalty; // minor penalty for kmult to keep zeros from annoying us if (global_defaults.signal_process_control.fit_region_kmult) - post_key_fit.MultiFlowSpecializedLevMarFitParameters ( NO_ADDITIONAL_WELL_ITERATIONS, STANDARD_POST_KEY_ITERATIONS, post_key_fit.fit_control.FitWellAll, post_key_fit.fit_control.FitRegionInit2, LARGER_LAMBDA , NO_NONCLONAL_PENALTY, flow_key, flow_block_size, flow_block_start ); + post_key_fit.MultiFlowSpecializedLevMarFitParameters ( NO_ADDITIONAL_WELL_ITERATIONS, STANDARD_POST_KEY_ITERATIONS, post_key_fit.fit_control.GetFitPacker("FitWellAll"), post_key_fit.fit_control.GetFitPacker(fittaue?"FitRegionInit2TauE":"FitRegionInit2"), LARGER_LAMBDA , NO_NONCLONAL_PENALTY, flow_key, flow_block_size, flow_block_start ); else - post_key_fit.MultiFlowSpecializedLevMarFitParameters ( NO_ADDITIONAL_WELL_ITERATIONS, STANDARD_POST_KEY_ITERATIONS, post_key_fit.fit_control.FitWellPostKey, post_key_fit.fit_control.FitRegionInit2, LARGER_LAMBDA , NO_NONCLONAL_PENALTY, flow_key, flow_block_size, flow_block_start ); + post_key_fit.MultiFlowSpecializedLevMarFitParameters ( NO_ADDITIONAL_WELL_ITERATIONS, STANDARD_POST_KEY_ITERATIONS, post_key_fit.fit_control.GetFitPacker("FitWellPostKey"), post_key_fit.fit_control.GetFitPacker(fittaue?"FitRegionInit2TauE":"FitRegionInit2"), LARGER_LAMBDA , NO_NONCLONAL_PENALTY, flow_key, flow_block_size, flow_block_start ); region_data->my_beads.AssignBarcodeState(true, global_defaults.signal_process_control.barcode_radius, global_defaults.signal_process_control.barcode_tie, flow_block_size, flow_block_start); if (global_defaults.signal_process_control.barcode_debug){ @@ -663,9 +665,9 @@ void SignalProcessingMasterFitter::PostKeyFitNoRegionalSampling (MultiFlowLevMar fit_timer.restart(); if (global_defaults.signal_process_control.fit_region_kmult) - post_key_fit.MultiFlowSpecializedLevMarFitParameters ( NO_ADDITIONAL_WELL_ITERATIONS, STANDARD_POST_KEY_ITERATIONS, post_key_fit.fit_control.FitWellAll, post_key_fit.fit_control.FitRegionFull, LARGER_LAMBDA, FULL_NONCLONAL_PENALTY, flow_key, flow_block_size, flow_block_start ); + post_key_fit.MultiFlowSpecializedLevMarFitParameters ( NO_ADDITIONAL_WELL_ITERATIONS, STANDARD_POST_KEY_ITERATIONS, post_key_fit.fit_control.GetFitPacker("FitWellAll"), post_key_fit.fit_control.GetFitPacker(fittaue?"FitRegionFullTauE":"FitRegionFull"), LARGER_LAMBDA, FULL_NONCLONAL_PENALTY, flow_key, flow_block_size, flow_block_start ); else - post_key_fit.MultiFlowSpecializedLevMarFitParameters ( NO_ADDITIONAL_WELL_ITERATIONS, STANDARD_POST_KEY_ITERATIONS, post_key_fit.fit_control.FitWellPostKey, post_key_fit.fit_control.FitRegionFull, LARGER_LAMBDA, FULL_NONCLONAL_PENALTY, flow_key, flow_block_size, flow_block_start ); + post_key_fit.MultiFlowSpecializedLevMarFitParameters ( NO_ADDITIONAL_WELL_ITERATIONS, STANDARD_POST_KEY_ITERATIONS, post_key_fit.fit_control.GetFitPacker("FitWellPostKey"), post_key_fit.fit_control.GetFitPacker(fittaue?"FitRegionFullTauE":"FitRegionFull"), LARGER_LAMBDA, FULL_NONCLONAL_PENALTY, flow_key, flow_block_size, flow_block_start ); elapsed_time += fit_timer.elapsed(); // last check @@ -683,8 +685,9 @@ void SignalProcessingMasterFitter::PostKeyFitWithRegionalSampling (MultiFlowLevM fit_timer.restart(); region_data->RezeroByCurrentTiming( flow_block_size ); // rezeroing?? + bool fittaue = global_defaults.signal_process_control.fitting_taue; - post_key_fit.MultiFlowSpecializedSampledLevMarFitParameters ( NO_ADDITIONAL_WELL_ITERATIONS, STANDARD_POST_KEY_ITERATIONS, post_key_fit.fit_control.FitWellPostKey, post_key_fit.fit_control.FitRegionInit2, LARGER_LAMBDA , NO_NONCLONAL_PENALTY, flow_key, flow_block_size, flow_block_start ); + post_key_fit.MultiFlowSpecializedSampledLevMarFitParameters ( NO_ADDITIONAL_WELL_ITERATIONS, STANDARD_POST_KEY_ITERATIONS, post_key_fit.fit_control.GetFitPacker("FitWellPostKey"), post_key_fit.fit_control.GetFitPacker(fittaue?"FitRegionInit2TauE":"FitRegionInit2"), LARGER_LAMBDA , NO_NONCLONAL_PENALTY, flow_key, flow_block_size, flow_block_start ); elapsed_time += fit_timer.elapsed(); // classify beads here? @@ -699,7 +702,7 @@ void SignalProcessingMasterFitter::PostKeyFitWithRegionalSampling (MultiFlowLevM post_key_fit.lm_state.ref_penalty_scale = global_defaults.signal_process_control.barcode_penalty; // big penalty for getting these wrong! post_key_fit.lm_state.kmult_penalty_scale = global_defaults.signal_process_control.kmult_penalty; // minor penalty for kmult to keep zeros from annoying us - post_key_fit.MultiFlowSpecializedSampledLevMarFitParameters ( NO_ADDITIONAL_WELL_ITERATIONS, STANDARD_POST_KEY_ITERATIONS, post_key_fit.fit_control.FitWellAll, post_key_fit.fit_control.FitRegionInit2, LARGER_LAMBDA , NO_NONCLONAL_PENALTY, flow_key, flow_block_size, flow_block_start ); + post_key_fit.MultiFlowSpecializedSampledLevMarFitParameters ( NO_ADDITIONAL_WELL_ITERATIONS, STANDARD_POST_KEY_ITERATIONS, post_key_fit.fit_control.GetFitPacker("FitWellAll"), post_key_fit.fit_control.GetFitPacker(fittaue?"FitRegionInit2TauE":"FitRegionInit2"), LARGER_LAMBDA , NO_NONCLONAL_PENALTY, flow_key, flow_block_size, flow_block_start ); region_data->my_beads.AssignBarcodeState(false, global_defaults.signal_process_control.barcode_radius, global_defaults.signal_process_control.barcode_tie, flow_block_size, flow_block_start); if (global_defaults.signal_process_control.barcode_debug){ region_data->my_beads.barcode_info.ReportClassificationTable(100+region_data->region->index); // show my classification @@ -710,14 +713,14 @@ void SignalProcessingMasterFitter::PostKeyFitWithRegionalSampling (MultiFlowLevM fit_timer.restart(); if (global_defaults.signal_process_control.fit_region_kmult){ - post_key_fit.MultiFlowSpecializedSampledLevMarFitParameters ( NO_ADDITIONAL_WELL_ITERATIONS, STANDARD_POST_KEY_ITERATIONS, post_key_fit.fit_control.FitWellAll, post_key_fit.fit_control.FitRegionFull, LARGER_LAMBDA, FULL_NONCLONAL_PENALTY, flow_key, flow_block_size, flow_block_start ); + post_key_fit.MultiFlowSpecializedSampledLevMarFitParameters ( NO_ADDITIONAL_WELL_ITERATIONS, STANDARD_POST_KEY_ITERATIONS, post_key_fit.fit_control.GetFitPacker("FitWellAll"), post_key_fit.fit_control.GetFitPacker(fittaue?"FitRegionFullTauE":"FitRegionFull"), LARGER_LAMBDA, FULL_NONCLONAL_PENALTY, flow_key, flow_block_size, flow_block_start ); } else { - post_key_fit.MultiFlowSpecializedSampledLevMarFitParameters ( NO_ADDITIONAL_WELL_ITERATIONS, STANDARD_POST_KEY_ITERATIONS, post_key_fit.fit_control.FitWellPostKey, post_key_fit.fit_control.FitRegionFull, LARGER_LAMBDA, FULL_NONCLONAL_PENALTY, flow_key, flow_block_size, flow_block_start ); + post_key_fit.MultiFlowSpecializedSampledLevMarFitParameters ( NO_ADDITIONAL_WELL_ITERATIONS, STANDARD_POST_KEY_ITERATIONS, post_key_fit.fit_control.GetFitPacker("FitWellPostKey"), post_key_fit.fit_control.GetFitPacker(fittaue?"FitRegionFullTauE":"FitRegionFull"), LARGER_LAMBDA, FULL_NONCLONAL_PENALTY, flow_key, flow_block_size, flow_block_start ); } elapsed_time += fit_timer.elapsed(); // last check if (barcode_flag){ - region_data->my_beads.AssignBarcodeState(false, global_defaults.signal_process_control.barcode_radius, global_defaults.signal_process_control.barcode_tie, flow_block_size, flow_block_start); + region_data->my_beads.AssignBarcodeState(false, global_defaults.signal_process_control.barcode_radius, global_defaults.signal_process_control.barcode_tie, flow_block_size, flow_block_start); } } @@ -775,7 +778,7 @@ void SignalProcessingMasterFitter::PostKeyFitAllWells ( double &elapsed_time, Ti } for (int i_train=0; i_trainmy_beads.my_mean_copy_count = region_data->my_beads.KeyNormalizeReads ( true, false, flow_block_size ); if (false){ float monitor_etbR = region_data->my_beads.etbRFromReads(); @@ -797,9 +800,9 @@ void SignalProcessingMasterFitter::PostKeyFitAllWells ( double &elapsed_time, Ti // only wells are fit here if (global_defaults.signal_process_control.fit_region_kmult) - all_wells_lev_mar_fit.MultiFlowSpecializedLevMarFitAllWells ( HAPPY_ALL_BEADS, all_wells_lev_mar_fit.fit_control.FitWellAll, LARGER_LAMBDA, FULL_NONCLONAL_PENALTY, flow_key, flow_block_size, flow_block_start ); + all_wells_lev_mar_fit.MultiFlowSpecializedLevMarFitAllWells ( HAPPY_ALL_BEADS, all_wells_lev_mar_fit.fit_control.GetFitPacker("FitWellAll"), LARGER_LAMBDA, FULL_NONCLONAL_PENALTY, flow_key, flow_block_size, flow_block_start ); else - all_wells_lev_mar_fit.MultiFlowSpecializedLevMarFitAllWells ( HAPPY_ALL_BEADS, all_wells_lev_mar_fit.fit_control.FitWellPostKey, LARGER_LAMBDA, FULL_NONCLONAL_PENALTY, flow_key, flow_block_size, flow_block_start ); + all_wells_lev_mar_fit.MultiFlowSpecializedLevMarFitAllWells ( HAPPY_ALL_BEADS, all_wells_lev_mar_fit.fit_control.GetFitPacker("FitWellPostKey"), LARGER_LAMBDA, FULL_NONCLONAL_PENALTY, flow_key, flow_block_size, flow_block_start ); elapsed_time += fit_timer.elapsed(); region_data->my_beads.my_mean_copy_count = region_data->my_beads.KeyNormalizeReads ( true, false, flow_block_size ); @@ -833,9 +836,9 @@ void SignalProcessingMasterFitter::FitAmplitudeAndDarkMatter ( MultiFlowLevMar & //@TODO: should I be skipping low-quality bead refits here because we'll be getting their amplitudes in the refinement phase? fit_timer.restart(); if (global_defaults.signal_process_control.enable_dark_matter) - fad_lev_mar_fit.MultiFlowSpecializedLevMarFitParameters ( NO_ADDITIONAL_WELL_ITERATIONS, STANDARD_POST_KEY_ITERATIONS, fad_lev_mar_fit.fit_control.FitWellAmpl, fad_lev_mar_fit.fit_control.FitRegionDarkness, BIG_LAMBDA , NO_NONCLONAL_PENALTY, flow_key, flow_block_size, flow_block_start ); + fad_lev_mar_fit.MultiFlowSpecializedLevMarFitParameters ( NO_ADDITIONAL_WELL_ITERATIONS, STANDARD_POST_KEY_ITERATIONS, fad_lev_mar_fit.fit_control.GetFitPacker("FitWellAmpl"), fad_lev_mar_fit.fit_control.GetFitPacker("FitRegionDarkness"), BIG_LAMBDA , NO_NONCLONAL_PENALTY, flow_key, flow_block_size, flow_block_start ); else - fad_lev_mar_fit.MultiFlowSpecializedLevMarFitParameters ( NO_ADDITIONAL_WELL_ITERATIONS, STANDARD_POST_KEY_ITERATIONS, fad_lev_mar_fit.fit_control.FitWellAmpl, fad_lev_mar_fit.fit_control.DontFitRegion, BIG_LAMBDA , NO_NONCLONAL_PENALTY, flow_key, flow_block_size, flow_block_start ); + fad_lev_mar_fit.MultiFlowSpecializedLevMarFitParameters ( NO_ADDITIONAL_WELL_ITERATIONS, STANDARD_POST_KEY_ITERATIONS, fad_lev_mar_fit.fit_control.GetFitPacker("FitWellAmpl"), NULL, BIG_LAMBDA , NO_NONCLONAL_PENALTY, flow_key, flow_block_size, flow_block_start ); elapsed_time += fit_timer.elapsed(); } @@ -961,11 +964,15 @@ void SignalProcessingMasterFitter::GuessCrudeAmplitude ( double &elapsed_time, T void SignalProcessingMasterFitter::FitTimeVaryingRegion ( double &elapsed_time, Timer &fit_timer, int flow_key, int flow_block_size, master_fit_type_table *table, int flow_block_start ) { MultiFlowLevMar tvr_lev_mar_fit( *this, flow_block_size, table ); + //example of using new interface + //table->addBkgModelFitType("NoCopydrift",{"TMidNuc","RatioDrift","TableEnd"}); + //tvr_lev_mar_fit.fit_control.AddFitPacker("NoCopydrift",region_data->time_c.npts(),flow_block_size); fit_timer.restart(); // >NOW< we allow any emphasis level given our crude estimates for emphasis region_data->AdaptiveEmphasis(); tvr_lev_mar_fit.ChooseSkipBeads ( true ); - tvr_lev_mar_fit.MultiFlowSpecializedLevMarFitParametersOnlyRegion ( 4, tvr_lev_mar_fit.fit_control.FitRegionTimeVarying, LARGER_LAMBDA , NO_NONCLONAL_PENALTY, flow_key, flow_block_size, flow_block_start ); + tvr_lev_mar_fit.MultiFlowSpecializedLevMarFitParametersOnlyRegion ( 4, tvr_lev_mar_fit.fit_control.GetFitPacker("FitRegionTimeVarying"), LARGER_LAMBDA , NO_NONCLONAL_PENALTY, flow_key, flow_block_size, flow_block_start ); + //tvr_lev_mar_fit.MultiFlowSpecializedLevMarFitParametersOnlyRegion ( 4, tvr_lev_mar_fit.fit_control.GetFitPacker("NoCopydrift"), LARGER_LAMBDA , NO_NONCLONAL_PENALTY, flow_key, flow_block_size, flow_block_start ); tvr_lev_mar_fit.ChooseSkipBeads ( false ); elapsed_time += fit_timer.elapsed(); } diff --git a/Analysis/BkgModel/SignalProcessingMasterFitter.h b/Analysis/BkgModel/SignalProcessingMasterFitter.h index 64c02adf..6fee1e42 100644 --- a/Analysis/BkgModel/SignalProcessingMasterFitter.h +++ b/Analysis/BkgModel/SignalProcessingMasterFitter.h @@ -372,6 +372,7 @@ class SignalProcessingMasterFitter GlobalWriter global_state; debug_collection my_debug; + DebugSaver debugSaver; // cache math that all bkgmodel objects need to execute PoissonCDFApproxMemo *math_poiss; diff --git a/Analysis/BkgModel/Writers/DebugWriter.cpp b/Analysis/BkgModel/Writers/DebugWriter.cpp index 9f367ce0..9a69f75a 100644 --- a/Analysis/BkgModel/Writers/DebugWriter.cpp +++ b/Analysis/BkgModel/Writers/DebugWriter.cpp @@ -1,12 +1,17 @@ /* Copyright (C) 2010 Ion Torrent Systems, Inc. All Rights Reserved */ #include "DebugWriter.h" +#include "BkgModel/Fitters/Complex/BkgFitMatDat.h" + #include #include #include #include "LinuxCompat.h" #include "RawWells.h" #include "SignalProcessingMasterFitter.h" +#include +#include "hdf5_hl.h" +#include #define BKG_MODEL_DEBUG_DIR "/bkg_debug/" @@ -340,3 +345,121 @@ void debug_collection::DumpRegionTrace (SignalProcessingMasterFitter &bkg, int f fflush (my_fp); } // end file exists } + + +hid_t DebugSaver::hdf_file_id = -1; +std::mutex dbg_mutex; + +void DebugSaver::DebugFileOpen( std::string& dirName ) +{ + std::lock_guard guard(dbg_mutex); + + if( hdf_file_id<0 ){ + hdf_file_id = 0; + std::string fname = dirName+"/optimizer.h5"; + hdf_file_id = H5Fcreate(fname.c_str(),H5F_ACC_TRUNC,H5P_DEFAULT,H5P_DEFAULT); + assert(hdf_file_id >= 0); + //CreateDataSet(); //move this somewhere else + } +} + +void DebugSaver::WriteData( const BkgFitMatrixPacker* reg_fit, reg_params& rp, int flow, const Region *region, const std::vector derivativeNames, int nbeads ) +{ + const arma::Mat *jtj = reg_fit->data->jtj; + const arma::Col *rhs = reg_fit->data->rhs; + const arma::Col *delta = reg_fit->data->delta; + + std::lock_guard guard(dbg_mutex); + if( hdf_file_id<0 ) + return; + + /* Save old error handler */ + herr_t ( *old_func ) ( hid_t, void * ); + void *old_client_data; + + H5Eget_auto ( H5E_DEFAULT, &old_func, &old_client_data ); + + /* Silence warnings by turning off error handling */ + H5Eset_auto ( H5E_DEFAULT, NULL, NULL ); + + hsize_t mat_dim[]={jtj->n_rows,jtj->n_cols}; + hsize_t vec_dim[]={delta->n_rows}; + char path[1024]; + snprintf(path, 1024, "regfit/flow_%d/r_%d_c_%d",flow,region->row,region->col); + + std::vector path_parts; + boost::split(path_parts,path,boost::is_any_of("/")); + + + hid_t dataset_id = hdf_file_id; + for( auto group_name=path_parts.begin(); group_name!=path_parts.end(); ++group_name ){ + + hid_t dataset_id_new = H5Gopen(dataset_id, group_name->c_str(),H5P_DEFAULT); + if( dataset_id_new<0) + dataset_id_new = H5Gcreate(dataset_id, group_name->c_str(), H5P_DEFAULT, H5P_DEFAULT, H5P_DEFAULT); + + if( dataset_id!=hdf_file_id) + H5Gclose(dataset_id); + dataset_id=dataset_id_new; + } + + //we store count in the branch corresponding to the current region + int iteration; + herr_t err = H5LTget_attribute_int(hdf_file_id,path,"count",&iteration); + if( err<0 ) + iteration = 0; + else + ++iteration; + + /* Restore previous error handler */ + H5Eset_auto ( H5E_DEFAULT, old_func, old_client_data ); + + err = H5LTset_attribute_int(hdf_file_id,path,"count",&iteration,1); + + std::string subgroup_name="iter_"; + dataset_id = H5Gcreate(dataset_id, (subgroup_name+std::to_string(iteration)).c_str(), H5P_DEFAULT, H5P_DEFAULT, H5P_DEFAULT); + + + //turn parameter names into comma separated string + std::stringstream ss; + for_each(derivativeNames.begin(), derivativeNames.end(), [&ss] (const std::string& s) { ss << s << ","; }); //uses lambda expression + std::string names = ss.str(); + names.pop_back(); //remove extra comma in the end + + H5LTmake_dataset(dataset_id, "jtj", 2, mat_dim, H5T_NATIVE_DOUBLE, jtj->memptr()); + H5LTset_attribute_string(dataset_id,"jtj","paramNames",names.c_str()); + H5LTmake_dataset(dataset_id, "rhs", 1, vec_dim, H5T_NATIVE_DOUBLE, rhs->memptr()); + H5LTset_attribute_string(dataset_id,"rhs","paramNames",names.c_str()); + H5LTmake_dataset(dataset_id, "delta", 1, vec_dim, H5T_NATIVE_DOUBLE, delta->memptr()); + H5LTset_attribute_string(dataset_id,"delta","paramNames",names.c_str()); + + //Save output + float* output= new float[reg_fit->getNumOutputs()]; + ss.str(""); + + for (int i=0;i < reg_fit->getNumOutputs();i++){ + // What is that right place? + float *dptr = (rp.*( reg_fit->getOuputList()[i].reg_params_func))(); + output[i] = dptr[reg_fit->getOuputList()[i].array_index]; + ss<getOuputList()[i].name<<","; + } + names = ss.str(); names.pop_back(); //remove extra comma in the end + vec_dim[0]=static_cast(reg_fit->getNumOutputs()); + H5LTmake_dataset(dataset_id, "output", 1, vec_dim, H5T_NATIVE_FLOAT, output); + H5LTset_attribute_string(dataset_id,"output","paramNames",names.c_str()); + delete[] output; + + vec_dim[0]=1; + H5LTmake_dataset(dataset_id, "nbeads", 1, vec_dim, H5T_NATIVE_INT, &nbeads); + H5Gclose(dataset_id); + H5Fflush(hdf_file_id, H5F_SCOPE_GLOBAL); +} + +DebugSaver::~DebugSaver() +{ + std::lock_guard guard(dbg_mutex); + if(hdf_file_id>=0){ + H5Fclose(hdf_file_id); + hdf_file_id=-1; + } +} diff --git a/Analysis/BkgModel/Writers/DebugWriter.h b/Analysis/BkgModel/Writers/DebugWriter.h index 5aa13055..596a161e 100644 --- a/Analysis/BkgModel/Writers/DebugWriter.h +++ b/Analysis/BkgModel/Writers/DebugWriter.h @@ -8,6 +8,9 @@ #include "BeadTracker.h" #include "RegionParams.h" #include "RegionTracker.h" +#include "BkgModel/Fitters/Complex/BkgFitMatrixPacker.h" +#include "hdf5.h" +#include class SignalProcessingMasterFitter; // forward definition @@ -23,7 +26,6 @@ class debug_collection FILE *region_only_trace_file; FILE *region_1mer_trace_file; FILE *region_0mer_trace_file; - debug_collection(); ~debug_collection(); void DebugFileClose(); @@ -37,4 +39,19 @@ class debug_collection int flow_block_size, int flow_block_start); }; +#define IF_OPTIMIZER_DEBUG( D, X ) { if( D->bkg_control.pest_control.bkgModelHdf5Debug > 3 ) {X;} } +class DebugSaver{ + +private: + static hid_t hdf_file_id; + + +public: + DebugSaver() {} //: hdf_file_id(-1) + ~DebugSaver(); + void DebugFileOpen(std::string& dirName); + void WriteData(const BkgFitMatrixPacker* reg_fit, reg_params &rp, int flow, const Region* region, const std::vector derivativeNames, int nbeads); +}; + + #endif // DEBUGWRITER_H diff --git a/Analysis/CMakeLists.txt b/Analysis/CMakeLists.txt index d7231f56..ccf4c26e 100644 --- a/Analysis/CMakeLists.txt +++ b/Analysis/CMakeLists.txt @@ -68,8 +68,8 @@ if(ION_USE_CUDA) #install(DIRECTORY ${PROJECT_BINARY_DIR}/../${cuda_toolkit_version}/lib64 # DESTINATION /usr/local/cuda) - install(FILES ${PROJECT_BINARY_DIR}/../${cuda_toolkit_version}/lib64/libcudart.so.7.5 - ${PROJECT_BINARY_DIR}/../${cuda_toolkit_version}/lib64/libcudart.so.7.5.18 + install(FILES ${PROJECT_BINARY_DIR}/../${cuda_toolkit_version}/lib64/libcudart.so.8.0 + ${PROJECT_BINARY_DIR}/../${cuda_toolkit_version}/lib64/libcudart.so.8.0.44 DESTINATION /usr/local/cuda/lib64) @@ -87,21 +87,22 @@ if(ION_USE_CUDA) install(PROGRAMS pynvml/pynvml_test.py DESTINATION /usr/local/bin) install(PROGRAMS pynvml/nvidia_smi_test.py DESTINATION /usr/local/bin) - CUDA_ADD_EXECUTABLE(xtalk_sim - xtalk_sim/xtalk_sim.cpp - xtalk_sim/DiffEqModel_Init.cpp - xtalk_sim/DiffEqModel.cpp - xtalk_sim/DelsqCUDA.cu - xtalk_sim/utils.cpp - xtalk_sim/xtalk_sim.cpp - xtalk_sim/sim_incorp_flux.cpp - xtalk_sim/WorkerInfoQueue.cpp) - - target_link_libraries(xtalk_sim pthread) - - add_dependencies(xtalk_sim cuda_toolkit) - - install(PROGRAMS ${PROJECT_BINARY_DIR}/xtalk_sim DESTINATION gpu) +# +# CUDA_ADD_EXECUTABLE(xtalk_sim +# xtalk_sim/xtalk_sim.cpp +# xtalk_sim/DiffEqModel_Init.cpp +# xtalk_sim/DiffEqModel.cpp +# xtalk_sim/DelsqCUDA.cu +# xtalk_sim/utils.cpp +# xtalk_sim/xtalk_sim.cpp +# xtalk_sim/sim_incorp_flux.cpp +# Util/WorkerInfoQueue.cpp) +# +# target_link_libraries(xtalk_sim pthread) +# +# add_dependencies(xtalk_sim cuda_toolkit) +# +# install(PROGRAMS ${PROJECT_BINARY_DIR}/xtalk_sim DESTINATION gpu) endif() @@ -259,9 +260,10 @@ add_library(ion-analysis LinuxCompat.cpp Stats.cpp - LevMarFitterV2.cpp + BkgModel/Fitters/LevMarFitterV2.cpp - mixed.cpp + ClonalFilter/mixed.cpp + ClonalFilter/polyclonal_filter.cpp # T0CalcMt.cpp BaseCaller/PIDloop.cpp @@ -365,9 +367,9 @@ endif() # armadillo doesn't provide those libs? if(ION_USE_MKL) - set(EXTRA_LIBS iomp5 mkl_intel_lp64) + set(EXTRA_LIBS m iomp5 mkl_intel_lp64) else() - set(EXTRA_LIBS blas lapack) + set(EXTRA_LIBS m blas lapack) endif() target_link_libraries(ion-analysis ${ION_HDF5_LIBS} dl ${ION_KMEANS_LIBS} ${ION_BOOST_LIBS} ${EXTRA_LIBS} file-io z) @@ -429,17 +431,17 @@ target_link_libraries(Thumbnail ion-analysis pthread dl) install(TARGETS Thumbnail DESTINATION bin) # If doing pca spline trial -add_executable(PcaSplineExample Image/PcaSplineExample.cpp ${PROJECT_BINARY_DIR}/IonVersion.cpp) -add_dependencies(PcaSplineExample IONVERSION) +#add_executable(PcaSplineExample Image/PcaSplineExample.cpp ${PROJECT_BINARY_DIR}/IonVersion.cpp) +#add_dependencies(PcaSplineExample IONVERSION) # If defined while running cmake look for mkl # e.g. -DMKL_INSTALL_PATH=/home/ionadmin/intel/composer_xe_2013_sp1.0.080/mkl -if (DEFINED MKL_INSTALL_PATH) - message("Compiling PcaSplineExample with mkl") - target_link_libraries(PcaSplineExample ion-analysis ${MKL_LINK_LIBS} -lpthread -lm dl z) -else() - message("Compiling PcaSplineExample with eigen only") - target_link_libraries(PcaSplineExample ion-analysis file-io pthread m dl z) -endif() +#if (DEFINED MKL_INSTALL_PATH) +# message("Compiling PcaSplineExample with mkl") +# target_link_libraries(PcaSplineExample ion-analysis ${MKL_LINK_LIBS} -lpthread -lm dl z) +#else() +# message("Compiling PcaSplineExample with eigen only") +# target_link_libraries(PcaSplineExample ion-analysis file-io pthread m dl z) +#endif() # If doing DfcCompr trial #add_executable(DfcComprExample Image/DfcComprExample.cpp ${PROJECT_BINARY_DIR}/IonVersion.cpp) @@ -535,12 +537,11 @@ include_directories("${PROJECT_SOURCE_DIR}/VariantCaller/Bookkeeping") include_directories("${PROJECT_SOURCE_DIR}/VariantCaller/Reads") include_directories("${PROJECT_SOURCE_DIR}/VariantCaller/Splice") include_directories("${PROJECT_SOURCE_DIR}/VariantCaller/Filter") +include_directories("${PROJECT_SOURCE_DIR}/VariantCaller/Consensus") include_directories("${PROJECT_SOURCE_DIR}/realignment") include_directories("${PROJECT_SOURCE_DIR}/Calibration") set(tvcSRCS - VariantCaller/IndelAssembly/IndelAssembly.cpp - VariantCaller/tvcutils/unify_vcf.cpp VariantCaller/VariantCaller.cpp VariantCaller/BAMWalkerEngine.cpp VariantCaller/OrderedBAMWriter.cpp @@ -549,27 +550,29 @@ set(tvcSRCS VariantCaller/HandleVariant.cpp VariantCaller/HotspotReader.cpp VariantCaller/MetricsManager.cpp - + VariantCaller/HypothesisEvaluator.cpp + VariantCaller/MolecularTag.cpp + VariantCaller/Bookkeeping/MiscUtil.cpp VariantCaller/Bookkeeping/ExtendParameters.cpp VariantCaller/Bookkeeping/InputStructures.cpp VariantCaller/Bookkeeping/VcfFormat.cpp VariantCaller/Reads/ExtendedReadInfo.cpp - + + VariantCaller/IndelAssembly/IndelAssembly.cpp + + VariantCaller/tvcutils/unify_vcf.cpp + VariantCaller/tvcutils/prepare_hotspots.cpp + VariantCaller/Splice/ErrorMotifs.cpp VariantCaller/Splice/LocalContext.cpp VariantCaller/Splice/ClassifyVariant.cpp - VariantCaller/Splice/ErrorMotifs.cpp VariantCaller/Splice/SpliceVariantHypotheses.cpp VariantCaller/Filter/DecisionTreeData.cpp VariantCaller/Filter/VariantAssist.cpp - VariantCaller/HypothesisEvaluator.cpp - - VariantCaller/MolecularTag.cpp - VariantCaller/EnsembleEval/DiagnosticJSON.cpp VariantCaller/EnsembleEval/BiasGenerator.cpp VariantCaller/EnsembleEval/SigmaGenerator.cpp @@ -579,6 +582,10 @@ set(tvcSRCS VariantCaller/EnsembleEval/StackEngine.cpp VariantCaller/EnsembleEval/CrossHypotheses.cpp + VariantCaller/Consensus/Consensus.cpp + VariantCaller/Consensus/FlowSpaceConsensus.cpp + VariantCaller/Consensus/ConsensusParameters.cpp + # TODO: Actually build vcflib as a static library and link to variant caller. # TODO2: Resolve bgzf.c collisions between vcflib and bamtools ${ION_VCFLIB_DIR}/Variant.cpp @@ -598,6 +605,7 @@ set(tvcSRCS Calibration/LinearCalibrationModel.cpp Calibration/FlowAlignment.cpp Util/OptArgs.cpp + AnalysisOrg/IO/OptBase.cpp realignment/Realigner.cpp ${ION_TS_EXTERNAL}/jsoncpp-src-amalgated0.6.0-rc1/jsoncpp.cpp ${PROJECT_BINARY_DIR}/IonVersion.cpp @@ -613,20 +621,29 @@ target_link_libraries(tvc ${ION_BAMTOOLS_LIBS} ${EXTRA_LIBS} z file-io pthread) add_dependencies(tvc IONVERSION bamtools armadillo_proj htslib_proj) install(TARGETS tvc DESTINATION bin) - ## tmol executable ## - -include_directories("${PROJECT_SOURCE_DIR}/VariantCaller/tmol") - -set(tmolSRCS - VariantCaller/tmol/tmol.cpp +# include_directories("${PROJECT_SOURCE_DIR}/VariantCaller/tmol") +#set(tmolSRCS +# VariantCaller/tmol/tmol.cpp +# ${PROJECT_BINARY_DIR}/IonVersion.cpp +#) +#add_executable(tmol ${tmolSRCS}) +#target_link_libraries(tmol ${ION_BAMTOOLS_LIBS} ${EXTRA_LIBS} z pthread) +#add_dependencies(tmol IONVERSION bamtools) +#install(TARGETS tmol DESTINATION bin) + +## vcfcomp executable ## +include_directories("${PROJECT_SOURCE_DIR}/VariantCaller/vcfcomp") + +set(vcfcompSRCS + VariantCaller/vcfcomp/vcfcomp.cpp ${PROJECT_BINARY_DIR}/IonVersion.cpp ) -add_executable(tmol ${tmolSRCS}) -target_link_libraries(tmol ${ION_BAMTOOLS_LIBS} ${EXTRA_LIBS} z pthread) -add_dependencies(tmol IONVERSION bamtools) -install(TARGETS tmol DESTINATION bin) +add_executable(vcfcomp ${vcfcompSRCS}) +target_link_libraries(vcfcomp ${ION_SAMTOOLS_LIBS} ${ION_HTSLIB_LIBS} z pthread) +add_dependencies(vcfcomp IONVERSION samtools) +install(TARGETS vcfcomp DESTINATION bin) # bbctools @@ -653,6 +670,8 @@ bbctools/src/RegionCoverage.cpp bbctools/src/RegionCoverage.h bbctools/src/RegionStatistics.cpp bbctools/src/RegionStatistics.h +bbctools/src/TrackReads.cpp +bbctools/src/TrackReads.h ) add_executable(bbctools ${bbctools_src}) @@ -697,6 +716,7 @@ add_executable(tvcutils VariantCaller/tvcutils/unify_vcf.cpp VariantCaller/tvcutils/split_vcf.cpp VariantCaller/TargetsManager.cpp + realignment/Realigner.cpp Util/OptArgs.cpp # Util/Utils.cpp ${ION_VCFLIB_DIR}/Variant.cpp @@ -929,7 +949,7 @@ if(GTEST_FOUND) add_executable(DualGaussMixModel_Test utest/DualGaussMixModel_Test.cpp) target_link_libraries(DualGaussMixModel_Test ion-analysis ${GTEST_BOTH_LIBRARIES} pthread) - add_test(DualGaussMixModelTest DualGaussMixModel_Test --gtest_output=xml:./) +# add_test(DualGaussMixModelTest DualGaussMixModel_Test --gtest_output=xml:./) # add_executable(ZeromerDiff_Test utest/ZeromerDiff_Test.cpp) # target_link_libraries(ZeromerDiff_Test ion-analysis ${GTEST_BOTH_LIBRARIES} ) @@ -983,6 +1003,10 @@ install(FILES "${PROJECT_SOURCE_DIR}/config/exclusionMask_324.bin" DESTINATION $ install(FILES "${PROJECT_SOURCE_DIR}/config/exclusionMask_p1.1.17.txt" DESTINATION ${ION_INSTALL_PREFIX}/config) install(FILES "${PROJECT_SOURCE_DIR}/config/exclusionMask_p1.0.19.txt" DESTINATION ${ION_INSTALL_PREFIX}/config) install(FILES "${PROJECT_SOURCE_DIR}/config/exclusionMask_p1.0.20.txt" DESTINATION ${ION_INSTALL_PREFIX}/config) +install(FILES "${PROJECT_SOURCE_DIR}/config/exclusionMask_PQ.txt" DESTINATION ${ION_INSTALL_PREFIX}/config) +install(FILES "${PROJECT_SOURCE_DIR}/config/exclusionMask_510.txt" DESTINATION ${ION_INSTALL_PREFIX}/config) +install(FILES "${PROJECT_SOURCE_DIR}/config/exclusionMask_520.txt" DESTINATION ${ION_INSTALL_PREFIX}/config) +install(FILES "${PROJECT_SOURCE_DIR}/config/exclusionMask_550.txt" DESTINATION ${ION_INSTALL_PREFIX}/config) install(FILES "${PROJECT_SOURCE_DIR}/config/exclusionMask_521.txt" DESTINATION ${ION_INSTALL_PREFIX}/config) install(FILES "${PROJECT_SOURCE_DIR}/config/exclusionMask_522.txt" DESTINATION ${ION_INSTALL_PREFIX}/config) install(FILES "${PROJECT_SOURCE_DIR}/config/exclusionMask_530.txt" DESTINATION ${ION_INSTALL_PREFIX}/config) @@ -1029,6 +1053,9 @@ install(FILES "${PROJECT_SOURCE_DIR}/config/phredTable.316v2.B5.Recal.h5" DESTIN install(FILES "${PROJECT_SOURCE_DIR}/config/phredTable.318.B5.h5" DESTINATION ${ION_INSTALL_PREFIX}/config) install(FILES "${PROJECT_SOURCE_DIR}/config/phredTable.318.B5.Recal.h5" DESTINATION ${ION_INSTALL_PREFIX}/config) +install(FILES "${PROJECT_SOURCE_DIR}/config/phredTable.318D.h5" DESTINATION ${ION_INSTALL_PREFIX}/config) +install(FILES "${PROJECT_SOURCE_DIR}/config/phredTable.318D.Recal.h5" DESTINATION ${ION_INSTALL_PREFIX}/config) + install(FILES "${PROJECT_SOURCE_DIR}/config/phredTable.900" DESTINATION ${ION_INSTALL_PREFIX}/config) install(FILES "${PROJECT_SOURCE_DIR}/config/phredTable.900.h5" DESTINATION ${ION_INSTALL_PREFIX}/config) install(FILES "${PROJECT_SOURCE_DIR}/config/phredTable.900.Recal" DESTINATION ${ION_INSTALL_PREFIX}/config) @@ -1162,6 +1189,7 @@ install(FILES "${PROJECT_SOURCE_DIR}/config/args_P2.3.1_beadfind.json" DESTINATI # #gopt parameters by chip type #gopt parameters +install(FILES "${PROJECT_SOURCE_DIR}/config/gopt_318D.param.json" DESTINATION ${ION_INSTALL_PREFIX}/config) install(FILES "${PROJECT_SOURCE_DIR}/config/gopt_314v2_Hi-Q.param.json" DESTINATION ${ION_INSTALL_PREFIX}/config) install(FILES "${PROJECT_SOURCE_DIR}/config/gopt_316v2_Hi-Q.param.json" DESTINATION ${ION_INSTALL_PREFIX}/config) install(FILES "${PROJECT_SOURCE_DIR}/config/gopt_318v2_Hi-Q.param.json" DESTINATION ${ION_INSTALL_PREFIX}/config) @@ -1205,6 +1233,8 @@ install(FILES "${PROJECT_SOURCE_DIR}/config/xtalk.trace.520.json" DESTINATION ${ install(FILES "${PROJECT_SOURCE_DIR}/config/xtalk.trace.521.json" DESTINATION ${ION_INSTALL_PREFIX}/config) install(FILES "${PROJECT_SOURCE_DIR}/config/xtalk.trace.522.json" DESTINATION ${ION_INSTALL_PREFIX}/config) install(FILES "${PROJECT_SOURCE_DIR}/config/xtalk.trace.530.json" DESTINATION ${ION_INSTALL_PREFIX}/config) +install(FILES "${PROJECT_SOURCE_DIR}/config/xtalk.550.well.settings.json" DESTINATION ${ION_INSTALL_PREFIX}/config) +install(FILES "${PROJECT_SOURCE_DIR}/config/xtalk.p2.3.1.well.settings.json" DESTINATION ${ION_INSTALL_PREFIX}/config) # Recalibration Panel install(FILES "${PROJECT_SOURCE_DIR}/config/datasets_calibration.json" DESTINATION ${ION_INSTALL_PREFIX}/config) @@ -1236,12 +1266,9 @@ endif() include(../buildTools/cmake/CMakeLists.cpack.txt) #% nice to automate this -set(CPACK_DEBIAN_PACKAGE_DEPENDS "libatlas3gf-base, - libblas3gf, - libc6, +set(CPACK_DEBIAN_PACKAGE_DEPENDS "libc6, libgcc1, libgfortran3, - liblapack3gf, libstdc++6, perl, ion-gpu (>=2.5.0)") diff --git a/Analysis/Calibration/Calibration.cpp b/Analysis/Calibration/Calibration.cpp index 88421c79..64a49ab4 100644 --- a/Analysis/Calibration/Calibration.cpp +++ b/Analysis/Calibration/Calibration.cpp @@ -117,7 +117,7 @@ int main (int argc, const char *argv[]) l_thread_time = ExecuteThreadedCalibrationTraining(calib_context); // Activate master linear model after every round of training - master_linear_model.CreateCalibrationModel(false); // make linear model + master_linear_model.CreateCalibrationModel(false); // make linear model master_linear_model.SetModelGainsAndOffsets(); // expand for use in basecalling calibration_thread_time += l_thread_time; diff --git a/Analysis/mixed.cpp b/Analysis/ClonalFilter/mixed.cpp similarity index 97% rename from Analysis/mixed.cpp rename to Analysis/ClonalFilter/mixed.cpp index 675dbf52..f9ac2339 100644 --- a/Analysis/mixed.cpp +++ b/Analysis/ClonalFilter/mixed.cpp @@ -212,7 +212,16 @@ bool fit_normals(vec mean[2], mat sgma[2], vec& alpha, const deque& ppf, // Initial guesses for two normal distributions: init(mean, sgma, alpha); if (opts.verbose) - print_dist(mean, sgma, alpha, converged, 0, opts.mixed_model_option); + print_dist(mean, sgma, alpha, converged, 0, opts.mixed_model_option); // XXX + + // increased stablity of iterative method: + // Add two points to the ppf, ssp deqeue corresponding to the a-priori cluster centers + std::deque my_ppf(ppf); + my_ppf.push_front(mean[0][0]); + my_ppf.push_front(mean[1][0]); + std::deque my_ssq(ssq); + my_ssq.push_front(mean[0][1]); + my_ssq.push_front(mean[1][1]); int max_iters = opts.max_iterations; int iteration = 1; @@ -225,7 +234,7 @@ bool fit_normals(vec mean[2], mat sgma[2], vec& alpha, const deque& ppf, mat new_sgma[2]; vec new_alpha; - calcMeanCovariance(new_sgma, new_mean, new_alpha, mean, sgma, alpha, ppf, ssq, opts.mixed_model_option); + calcMeanCovariance(new_sgma, new_mean, new_alpha, mean, sgma, alpha, my_ppf, my_ssq, opts.mixed_model_option); if(not is_pos_def(sgma[0]) or not is_pos_def(sgma[1])) { not_pos_def = true; @@ -290,10 +299,11 @@ void calcMeanCovariance(mat new_sgma[2], vec new_mean[2], vec &new_alpha, const case 0:{//common covariance bivariate_gaussian clone_dist(mean[0], sgma[0]); bivariate_gaussian mixed_dist(mean[1], sgma[1]); + // Re-estimate parameters for each distribution: int nsamp = ppf.size(); vec sumw(2); - sumw.fill(0.0); + sumw.fill(0.5); mat sum2(2,2); sum2.fill(0.0); @@ -317,11 +327,11 @@ void calcMeanCovariance(mat new_sgma[2], vec new_mean[2], vec &new_alpha, const vec q(2); q[0] = alpha[0] * clone_dist.pdf(x); q[1] = alpha[1] * mixed_dist.pdf(x); - vec w = q / sum(q); // Skip outliers: - if(not w.is_finite()) - continue; + if(sum(q) < 1e-20) + continue; + vec w = q / sum(q); // Running sums for moments are weighted: sumw += w; @@ -647,15 +657,3 @@ ostream& operator<<(ostream& out, const filter_counts& c) //Is it possible to try different models and find the one fitting the data best? -PolyclonalFilterOpts::PolyclonalFilterOpts() -{ - mixed_first_flow = 12; - mixed_last_flow = 72; - max_iterations = 30; - mixed_model_option = 0; - mixed_stringency = 0.5; - use_last_iter_params = true; - verbose = false; - filter_extreme_ppf_only = false; -} - diff --git a/Analysis/mixed.h b/Analysis/ClonalFilter/mixed.h similarity index 93% rename from Analysis/mixed.h rename to Analysis/ClonalFilter/mixed.h index 332993fa..1d6f622d 100644 --- a/Analysis/mixed.h +++ b/Analysis/ClonalFilter/mixed.h @@ -102,7 +102,11 @@ class clonal_filter if(ppf <_ppf_cutoff){ double clonal_pdf = _clonal.pdf (x); double mixed_pdf = _mixed.pdf (x); - return clonal_pdf / (mixed_pdf + clonal_pdf) > stringency; + // Avoid divide by zero: If mixed probablity is too small, we'll just call it clonal. + if (mixed_pdf < 1e-256) + return true; + else + return clonal_pdf / (mixed_pdf + clonal_pdf) > stringency; } else return false; diff --git a/Analysis/ClonalFilter/polyclonal_filter.cpp b/Analysis/ClonalFilter/polyclonal_filter.cpp new file mode 100644 index 00000000..2bfe0ef6 --- /dev/null +++ b/Analysis/ClonalFilter/polyclonal_filter.cpp @@ -0,0 +1,107 @@ +/* Copyright (C) 2016 Thermo Fisher Scientific, All Rights Reserved */ +#include "polyclonal_filter.h" + +PolyclonalFilterOpts::PolyclonalFilterOpts() +{ + SetDefaults(); +} + + +void PolyclonalFilterOpts::PrintHelp(bool analysis_call) +{ + printf (" Polyclonal Filter Options:\n"); + + if (analysis_call) + printf (" --clonal-filter-bkgmodel BOOL enable polyclonal filter during signal processing [on]\n"); + else{ + printf (" --clonal-filter-solve on/off apply polyclonal filter [off]\n"); + printf (" --clonal-filter-tf on/off apply polyclonal filter to TFs [off]\n"); + printf (" --clonal-filter-maxreads INT maximum number of library reads used for polyclonal filter training [100000]\n"); + } + + printf (" --mixed-first-flow INT mixed first flow of polyclonal filter [12]\n"); + printf (" --mixed-last-flow INT mixed last flow of polyclonal filter [72]\n"); + printf (" --max-iterations INT max iterations of polyclonal filter [30]\n"); + printf (" --mixed-model-option INT mixed model option of polyclonal filter [0]\n"); + printf (" --mixed-stringency DOUBLE mixed stringency of polyclonal filter [0.5]\n"); + printf (" --clonal-filter-debug BOOL enable polyclonal filter debug output [off]\n"); + printf (" --clonal-filter-use-last-iter-params BOOL use last EM iteration cluster parameters if no convergence [on]\n"); + printf (" --filter-extreme-ppf-only BOOL Skip polyclonal filter training and filter for extreme ppf only [off]\n"); + printf ("\n"); +} + +//----------------------------------------------------------------------------- + +void PolyclonalFilterOpts::SetDefaults() +{ + enable = false; + + filter_clonal_enabled_tfs = false; + filter_clonal_enabled_lib = false; + filter_clonal_maxreads = 100000; + + mixed_first_flow = 12; + mixed_last_flow = 72; + max_iterations = 30; + mixed_model_option = 0; + mixed_stringency = 0.5; + use_last_iter_params = true; + verbose = false; + filter_extreme_ppf_only = false; +} + +//----------------------------------------------------------------------------- + +void PolyclonalFilterOpts::Disable() +{ + enable = false; + filter_clonal_enabled_tfs = false; + filter_clonal_enabled_lib = false; +} + +//----------------------------------------------------------------------------- + +void PolyclonalFilterOpts::SetOpts(bool analysis_call, OptArgs &opts, Json::Value& json_params, int num_flows) +{ + SetDefaults(); + + // Analysis only option + if (analysis_call) + enable = RetrieveParameterBool(opts, json_params, '-', "clonal-filter-bkgmodel", true); + else { + // Basecaller only option + filter_clonal_enabled_tfs = RetrieveParameterBool(opts, json_params, '-', "clonal-filter-tf", false); + filter_clonal_enabled_lib = RetrieveParameterBool(opts, json_params,'-', "clonal-filter-solve", false); + filter_clonal_maxreads = RetrieveParameterInt (opts, json_params,'-', "clonal-filter-maxreads", 100000); + enable = filter_clonal_enabled_tfs or filter_clonal_enabled_lib; + } + + // Joint Analysis and BaseCaller Options + mixed_first_flow = RetrieveParameterInt(opts, json_params, '-', "mixed-first-flow", 12); + mixed_last_flow = RetrieveParameterInt(opts, json_params, '-', "mixed-last-flow", 72); + // Check prevents memory overrun and segfaults + if (mixed_last_flow > num_flows){ + cerr << "PolyclonalFilterOpts WARNING: mixed-last-flow is larger than number of flows in the run: " << num_flows << ". Disabling polyclonal filter." << endl; + Disable(); + } + + max_iterations = RetrieveParameterInt(opts, json_params, '-', "max-iterations", 30); + mixed_model_option = RetrieveParameterInt(opts, json_params, '-', "mixed-model-option", 0); + verbose = RetrieveParameterBool(opts, json_params, '-', "clonal-filter-debug", false); + use_last_iter_params = RetrieveParameterBool(opts, json_params, '-', "clonal-filter-use-last-iter-params", true); + filter_extreme_ppf_only = RetrieveParameterBool(opts, json_params, '-', "filter-extreme-ppf-only", false); + + // Transform stringency to log scale + double stringency = min(1.0, max(0.0, RetrieveParameterDouble(opts, json_params, '-', "mixed-stringency", 0.5))); + if(stringency > 0.5){ + mixed_stringency = 0.5 * std::log10((stringency - 0.5)*18 + 1) + 0.5; + } + else if (stringency < 0.5){ + mixed_stringency = 0.5 - 0.5 * std::log10(( 0.5 - stringency)*18 + 1); + } + else { + mixed_stringency = 0.5; + } + +} + diff --git a/Analysis/ClonalFilter/polyclonal_filter.h b/Analysis/ClonalFilter/polyclonal_filter.h new file mode 100644 index 00000000..68464dc0 --- /dev/null +++ b/Analysis/ClonalFilter/polyclonal_filter.h @@ -0,0 +1,56 @@ +/* Copyright (C) 2014 Ion Torrent Systems, Inc. All Rights Reserved */ + +#ifndef POLYCLONAL_FILTER_H +#define POLYCLONAL_FILTER_H + +#include "json/json.h" +#include "IO/OptBase.h" +#include "Util/Serialization.h" + +class PolyclonalFilterOpts { + public: + PolyclonalFilterOpts(); + + static void PrintHelp(bool anaysis_call); + + bool enable; + bool filter_clonal_enabled_tfs; + bool filter_clonal_enabled_lib; + int filter_clonal_maxreads; + int mixed_first_flow; + int mixed_last_flow; + int max_iterations; + int mixed_model_option; + double mixed_stringency; + bool verbose; + bool use_last_iter_params; + bool filter_extreme_ppf_only; + + void SetOpts(bool analysis_call, OptArgs &opts, Json::Value& json_params, int num_flows); + void Disable(); + +private: + + void SetDefaults(); + + friend class boost::serialization::access; + template + void serialize(Archive& ar, const unsigned int version) + { + ar + & enable + & filter_clonal_enabled_tfs + & filter_clonal_enabled_lib + & filter_clonal_maxreads + & mixed_first_flow + & mixed_last_flow + & max_iterations + & mixed_model_option + & mixed_stringency + & verbose + & use_last_iter_params + & filter_extreme_ppf_only; + } +}; + +#endif // POLYCLONAL_FILTER_H diff --git a/Analysis/DataViewer/AlignmentSpatial.cpp b/Analysis/DataViewer/AlignmentSpatial.cpp new file mode 100644 index 00000000..45be8046 --- /dev/null +++ b/Analysis/DataViewer/AlignmentSpatial.cpp @@ -0,0 +1,370 @@ +/* Copyright (C) 2016 Ion Torrent Systems, Inc. All Rights Reserved */ +#include "AlignmentSpatial.h" +#include +#include +#include +#include "Calibration/FlowAlignment.h" + +using namespace std; +using namespace BamTools; + + +AlignmentSpatial::AlignmentSpatial(QWidget *parent): SpatialPlot(parent) +{ +} + + +// copied from ion_util.c +int ion_readname_to_xy(const char *readname, int *x, int *y) +{ + int i, val, state; + /* states: + 0 - skipping over read name (before first colon) + 1 - reading in x value (before second colon) + 2 - reading in y value (after second colon) + */ + for(i=val=state=0;'\0' != readname[i];i++) { + if(':' == readname[i]) { + if(1 == state) { + (*y) = val; + } + state++; + val = 0; + } + else if('0' <= readname[i] && readname[i] <= '9') { + val *= 10; + val += (int32_t)(readname[i] - '0'); + } + } + if(2 == state) { + (*x) = val; + return 1; + } + else { + return 0; + } +} + +void AlignmentSpatial::SetOption(QString txt, int state) +{ + qDebug() << __PRETTY_FUNCTION__ << ": " << txt << state; + + if(txt == "Mask UnAligned") + SetMask(0,state); + else if(txt == "Mask Aligned") + SetMask(1,state); + + render(); +} + +void AlignmentSpatial::SetMask(int aligned, int state) +{ + if(mMask){ + + // set the global mask bits for aligned or unaligned reads + if(!state){ + // clear the mask bits for all reads + for(int i=0;i<(rows*cols);i++) + mMask[i] &= ~0x1; + }else{ + for(int i=0;i<(rows*cols);i++){ + if((aligned && (out[i] > 0)) || (!aligned && (out[i] == 0))) + mMask[i] |= 0x1; + } + } + } +} + +void AlignmentSpatial::doConvert(int &loading) +{ + + if(fname == ""){ + free(out); + out=NULL; + last_fname = _fname; + traces_len=0; + } + if (last_fname != _fname && !loading){ + loading=0; + // load a new BAM file + printf("triggered %s %s\n",last_fname.toLatin1().data(),_fname.toLatin1().data()); + fflush(stdout); + last_fname = _fname; + if(out) + free(out); + out=NULL; + + // region size + int nbytes=sizeof(out[0])*rows*cols; + out = (int32_t *)(int32_t *)malloc(nbytes); + +// char tmpName[4096]; +// strcpy(tmpName,fname.toStdString().data()); +// char *ptr = tmpName; +// char *last=NULL; +// while((ptr = strstr(ptr,"/"))){ +// last = ptr; +// ptr++; +// } +// if(last) +// *last = 0; + + _fname = fname;//QString(tmpName); + QDir dir(_fname); + qDebug() << "Dir: " << _fname; + foreach(QFileInfo item, dir.entryInfoList() ){ + if(item.isFile()){ + QString ifn = item.canonicalFilePath(); + char EntryName[4096]; + strcpy(EntryName,ifn.toUtf8().constData()); + qDebug() << "File: " << ifn << " - " << EntryName; +// printf(" File: %s\n",EntryName); + fflush(stdout); + char *bamPtr = strstr(EntryName,".bam"); + if(bamPtr && bamPtr[4] == 0){ + printf("Loading: %s\n",EntryName); + fflush(stdout); + ReadFile(EntryName); + } + } + } + +// ReadFile(fn); + + traces_len=1; + for(int i=0;iLoadFile(cols, rows, Block_X, Block_Y, out, fn, &loading, &seqIndx, &alignments); + if(gbl > (sizeof(threads)/sizeof(threads[0]))) + gbl=0; + +#if 0 + BamReader reader; + if ( !reader.Open(fn) ) { + printf("Could not open input BAM file %s\n",fn); + loading=0; + return; + } + + // region position + int RawExpmt_Y=Block_Y; + int RawExpmt_X=Block_X; + + BamAlignment al; + while ( reader.GetNextAlignment(al) ) { + int x,y; + ion_readname_to_xy(al.Name.c_str(), &x, &y); + int region_x = x - RawExpmt_X; + int region_y = y - RawExpmt_Y; + if (region_x >= 0 && region_x < cols && region_y >=0 && region_y < rows){ + out[region_y*cols + region_x] = al.AlignedBases.length(); + //cout << x << ":" << y << " length " << al.Length << endl; + } + } + reader.Close(); +#endif +} + +float AlignmentSpatial::Get_Data(int frame, int y, int x) +{ + (void)frame; + float rc = 0; + + if(out && y < rows && x < cols){ + rc = out[/*frame*rows*cols +*/ y*cols + x]; + } + return rc; +} + +BamAlignment* AlignmentSpatial::Get_Alignment(int y, int x) +{ + int found = std::find(seqIndx.begin(), seqIndx.end(), y*cols + x) - seqIndx.begin(); + if (found < (int)seqIndx.size()){ + return &alignments[found]; + } + return NULL; +} + +void AlignmentSpatial::Get_ReferenceSeq(BamAlignment *al, string &qseq, string &tseq){ + // tseq: refernce (target) bases for aligned portion of the read + // qseq: read (query) bases for aligned portion of the read + string md; + string tseq_bases; + string qseq_bases; + string pretty_tseq; + string pretty_qseq; + string pretty_aln; + unsigned int left_sc, right_sc; + + al->GetTag("MD", md); + RetrieveBaseAlignment(al->QueryBases, al->CigarData, md, tseq_bases, qseq_bases, + pretty_tseq, pretty_qseq, pretty_aln, left_sc, right_sc); + qseq = pretty_qseq; + tseq = pretty_tseq; +} + +void AlignmentSpatial::UpdateTracePlot() +{ + printf("%s\n",__FUNCTION__); + + QFont myfont("QFont::Courier", 8); + myfont.setStyleHint(QFont::TypeWriter); + _mTracePlot->clearItems(); + + if (traces_len) + { + for(int trc=0;trc=0) && (traces[trc].y > 0)) + { + // draw graph line + QVector x(2); + QVector y(2); + x[0] = 0; x[1] = 0.2; + y[0] = y[1] = MAX_NUM_TRACES - trc; + + _mTracePlot->graph(trc)->setData(x, y); + QString name="Y" + QString::number(traces[trc].x) + "_X" + QString::number(traces[trc].y); + _mTracePlot->graph(trc)->setName(name); + _mTracePlotSet[trc]=1; + + // add sequence text + BamAlignment *al= Get_Alignment(traces[trc].y, traces[trc].x); + if (al){ + QCPItemText *seqText = new QCPItemText(_mTracePlot); + _mTracePlot->addItem(seqText); + seqText->position->setCoords(x[1], y[0]); + seqText->setFont(myfont); + seqText->setPositionAlignment(Qt::AlignLeft|Qt::AlignVCenter); + + string qseq, tseq; + Get_ReferenceSeq(al, qseq, tseq); + string text = qseq + "\n" + tseq; + cout << text << endl; + seqText->setText(text.c_str()); + } + } + } + } + + _mTracePlot->xAxis->setRange(0, 10); + _mTracePlot->yAxis->setRange(0, MAX_NUM_TRACES+0.5); + _mTracePlot->replot(); +} + + +AlignmentRdrThread::AlignmentRdrThread(QObject *parent) + : QThread(parent) +{ +} + +//! [0] + +//! [1] +AlignmentRdrThread::~AlignmentRdrThread() +{ + mutex.lock(); + abort = true; + condition.wakeOne(); + mutex.unlock(); + + wait(); +} +//! [1] + + +//! [2] +void AlignmentRdrThread::LoadFile(int _w, int _h, int _startX, int _startY, int32_t *_out, char *_fileName, int *_loading, vector *_seqIndx, vector *_alignments) +{ + w=_w; + h=_h; + startX=_startX; + startY=_startY; + out=_out; + loading=_loading; + seqIndx= _seqIndx; + alignments= _alignments; + + strcpy(fileName,_fileName); + + QMutexLocker locker(&mutex); + + if (!isRunning()) { + __sync_add_and_fetch(loading,1); + start(LowPriority); + } else { + //restart = true; + condition.wakeOne(); + } +} +//! [2] + +void AlignmentRdrThread::Abort() +{ + abort=true; + mutex.unlock(); +} + +void AlignmentRdrThread::run() +{ +// forever { +// mutex.lock(); + if(abort) + return; // application is closed +// mParent->copyData(); +// mutex.unlock(); + + + BamReader reader; + if ( !reader.Open(fileName) ) { + printf("Could not open input BAM file %s\n",fileName); + //loading=0; + //return; + } + else{ + + // region position +// int RawExpmt_Y=Block_Y; +// int RawExpmt_X=Block_X; + + BamAlignment al; + while ( !abort && reader.GetNextAlignment(al) ) { + int x,y; + ion_readname_to_xy(al.Name.c_str(), &x, &y); + int region_x = x - startX; + int region_y = y - startY; + if (region_x >= 0 && region_x < w && region_y >=0 && region_y < h){ + out[region_y*w + region_x] = al.AlignedBases.length(); +// seqIndx->push_back(region_y*w + region_x); +// alignments->push_back(al); + } + } + reader.Close(); + printf("done loading %s\n",fileName); + } + + __sync_sub_and_fetch (loading,1); + + + +// mutex.lock(); +// if (!restart) +// condition.wait(&mutex); +// restart = false; +// mutex.unlock(); +// } +} + + + diff --git a/Analysis/DataViewer/AlignmentSpatial.h b/Analysis/DataViewer/AlignmentSpatial.h new file mode 100644 index 00000000..ea21a57f --- /dev/null +++ b/Analysis/DataViewer/AlignmentSpatial.h @@ -0,0 +1,73 @@ +/* Copyright (C) 2016 Ion Torrent Systems, Inc. All Rights Reserved */ +#ifndef ALIGNMENTSPATIAL_H +#define ALIGNMENTSPATIAL_H + +#include +#include +#include "SpatialPlot.h" +#include "qcustomplot.h" +#include "api/BamReader.h" + +class AlignmentRdrThread; + +class AlignmentSpatial : public SpatialPlot +{ +public: + AlignmentSpatial(QWidget *parent); + void doConvert(int &loading); + void SetOption(QString option, int state); + +protected: + float Get_Data(int frame, int y, int x); + void ReadFile(char *fn); + void UpdateTracePlot(); + +private: + int32_t *out=NULL; + AlignmentRdrThread *threads[100]={NULL}; + std::vector seqIndx; + std::vector alignments; + BamTools::BamAlignment *Get_Alignment(int y, int x); + void Get_ReferenceSeq(BamTools::BamAlignment *al, std::string &qseq, std::string &tseq); + void SetMask(int aligned, int state); +}; + + +class AlignmentRdrThread : public QThread +{ +// Q_OBJECT + +public: + AlignmentRdrThread(QObject *parent = 0); + ~AlignmentRdrThread(); + void Abort(); + void LoadFile(int w, int h, int startX, int startY, int32_t *out, char *_fileName, int *_loading, std::vector *_seqIndx, std::vector *_alignments); +// void SetParent(SpatialPlot *_parent); + + +signals: +// void renderedImage(const QImage &image); + +protected: + void run() Q_DECL_OVERRIDE; + +private: + + int w; + int h; + int startX; + int startY; + int32_t *out; + int *loading=NULL; + char fileName[4096]; + std::vector *seqIndx; + std::vector *alignments; + + + QMutex mutex; + QWaitCondition condition; + bool abort=0; +}; + + +#endif // ALIGNMENTSPATIAL_H diff --git a/Analysis/DataViewer/AlignmentTab.cpp b/Analysis/DataViewer/AlignmentTab.cpp new file mode 100644 index 00000000..2cdddde6 --- /dev/null +++ b/Analysis/DataViewer/AlignmentTab.cpp @@ -0,0 +1,32 @@ +/* Copyright (C) 2016 Ion Torrent Systems, Inc. All Rights Reserved */ +#include "AlignmentTab.h" + +static const maskCheckBox_t mcbt1[] = { + {"Mask UnAligned",1,Qt::Unchecked}, + {"Mask Aligned",2,Qt::Unchecked} +}; + +AlignmentTab::AlignmentTab(QString _mName, Dialog *_mParent, QWidget *parent) + : ModelTab(_mName, _mParent, parent) +{ + mSpatialPlot = new AlignmentSpatial(this); + + QGridLayout *grid = new QGridLayout; + grid->addWidget(MakeSlider("flows"),0,0); + grid->addWidget(MakeTraceCB(),1,0); + grid->addWidget(MakeList(mcbt1,sizeof(mcbt1)/sizeof(mcbt1[0])),0,1,2,1); + grid->setRowStretch(0,0); + grid->setRowStretch(1,0); + + grid->addWidget(mSpatialPlot,2,0,1,4); + grid->addWidget(MakeTracePlot(1),3,0,1,4); + + // Set the graph styles to no line + for(int i=0;igraph(i)->setLineStyle(QCPGraph::lsNone); + mtracePlot->graph(i)->setScatterStyle(QCPScatterStyle(QCPScatterStyle::ssCircle,4)); + } + + setLayout(grid); +} + diff --git a/Analysis/DataViewer/AlignmentTab.h b/Analysis/DataViewer/AlignmentTab.h new file mode 100644 index 00000000..bd742308 --- /dev/null +++ b/Analysis/DataViewer/AlignmentTab.h @@ -0,0 +1,27 @@ +/* Copyright (C) 2016 Ion Torrent Systems, Inc. All Rights Reserved */ +#ifndef ALIGNMENTTAB_H +#define ALIGNMENTTAB_H + +#include +#include +#include "dialog.h" +#include "modeltab.h" +#include "AlignmentSpatial.h" + +class Dialog; + +class AlignmentTab : public ModelTab +{ + Q_OBJECT +public: + explicit AlignmentTab(QString _mName, Dialog *_mParent, QWidget *parent = 0); + +signals: + +public slots: + +private: + +}; + +#endif // ALIGNMENTTAB_H diff --git a/Analysis/DataViewer/BfMaskSpatial.cpp b/Analysis/DataViewer/BfMaskSpatial.cpp new file mode 100644 index 00000000..d27b111b --- /dev/null +++ b/Analysis/DataViewer/BfMaskSpatial.cpp @@ -0,0 +1,125 @@ +/* Copyright (C) 2016 Ion Torrent Systems, Inc. All Rights Reserved */ +#include "BfMaskSpatial.h" +#include "RawWells.h" +#include +#include + +using namespace std; + +BfMaskSpatial::BfMaskSpatial(QWidget *parent): SpatialPlot(parent) +{ +} + +void BfMaskSpatial::addMask(uint mask) +{ + qDebug() << __PRETTY_FUNCTION__ << ": " << mask; + mMaskVal |= mask; + render(); +} + +void BfMaskSpatial::RemoveMask(uint mask) +{ + qDebug() << __PRETTY_FUNCTION__ << ": " << mask; + mMaskVal &= ~mask; + render(); +} + +void BfMaskSpatial::SetMask(int inverted, int state) +{ + if(mMask){ + // set the global mask bits for aligned or unaligned reads + if(!state){ + // clear the mask bits for all reads + for(int i=0;i<(rows*cols);i++) + mMask[i] &= ~0x2; + }else{ + for(int i=0;i<(rows*cols);i++){ + if((inverted && ((out[i] & mMaskVal) == 0)) || (!inverted && (out[i] & mMaskVal))) + mMask[i] |= 0x2; + } + } + } +} + +void BfMaskSpatial::doConvert(int &loading) +{ + if(fname == ""){ + free(out); + out=refMask=NULL; + last_fname = _fname; + traces_len=0; + } + if (last_fname != _fname){ + loading=1; + // load a new dat file + printf("triggered %s %s\n",last_fname.toLatin1().data(),_fname.toLatin1().data()); + last_fname = _fname; + if(out) + free(out); + out=refMask=NULL; + + QByteArray ba = fname.toLatin1(); + char * fn = ba.data(); + + { + //Note, fillMask is optional argument w/ default of true. + FILE *in = fopen ( fn, "rb" ); + if(in){ + int elements_read = fread ( &rows, sizeof ( rows ), 1, in ); + assert ( elements_read == 1 ); + elements_read = fread ( &cols, sizeof ( cols ), 1, in ); + assert ( elements_read == 1 ); + int nbytes = sizeof(out[0])*rows*cols; + out = refMask = (uint16_t *)malloc(nbytes); + elements_read = fread ( out, nbytes, 1, in ); + assert ( elements_read == 1 ); + fclose ( in ); + } + } + traces_len=2; + for(int i=0;i +#include "SpatialPlot.h" +#include "qcustomplot.h" +#include "modeltab.h" + + +class BfMaskSpatial : public SpatialPlot +{ +public: + BfMaskSpatial(QWidget *parent); + void doConvert(int &loading); + void addMask(uint mask); + void RemoveMask(uint mask); + void SetOption(QString txt, int state); + void SetOptions(const maskCheckBox_t *_mcbt, int _mNitems); + +protected: + virtual float Get_Data(int idx, int y, int x); + +private: + void SetMask(int aligned, int state); + uint16_t *out=NULL; + uint mMaskVal=0; + const maskCheckBox_t *mcbt=NULL; + int mNitems=-1; +}; + +#endif // BFMASKSPATIAL_H diff --git a/Analysis/DataViewer/BfMaskTab.cpp b/Analysis/DataViewer/BfMaskTab.cpp new file mode 100644 index 00000000..d2ba6765 --- /dev/null +++ b/Analysis/DataViewer/BfMaskTab.cpp @@ -0,0 +1,49 @@ +/* Copyright (C) 2016 Ion Torrent Systems, Inc. All Rights Reserved */ +#include "BfMaskTab.h" +#include "Mask.h" + + + +static const maskCheckBox_t mcbt1[] = { + {"MaskEmpty",0,Qt::Checked}, + {"MaskBead",1,Qt::Checked}, + {"MaskLive",2,Qt::Checked}, + {"MaskDud",3,Qt::Checked}, + {"MaskReference",4,Qt::Checked}, + {"MaskTF",5,Qt::Checked}, + {"MaskLib",6,Qt::Checked}, + {"MaskPinned",7,Qt::Unchecked}, + {"MaskIgnore",8,Qt::Unchecked}, + {"MaskWashout",9,Qt::Unchecked}, + {"MaskExclude",10,Qt::Unchecked}, +// {"MaskKeypass",11,Qt::Unchecked}, +// {"MaskFilteredBadKey",12,Qt::Unchecked}, +// {"MaskFilteredShort",13,Qt::Unchecked}, +// {"MaskFilteredBadPPF",14,Qt::Unchecked}, +// {"MaskFilteredBadResidual",15,Qt::Unchecked} + {"ApplyMask",16,Qt::Unchecked}, + {"ApplyInvMask",17,Qt::Unchecked}, + +}; + +BfMaskTab::BfMaskTab(QString mName, Dialog *_mParent, QWidget *parent) + : ModelTab(mName,_mParent,parent) +{ + mBfSpatialPlot = new BfMaskSpatial(this); + mBfSpatialPlot->SetOptions(mcbt1,sizeof(mcbt1)/sizeof(mcbt1[0])); + mSpatialPlot = mBfSpatialPlot; + + QGridLayout *grid = new QGridLayout; + grid->addWidget(MakeSlider(""),0,0); + grid->addWidget(MakeTraceCB(),1,0); + grid->addWidget(MakeList(mcbt1,sizeof(mcbt1)/sizeof(mcbt1[0])),0,1,2,1); + grid->setRowStretch(0,0); + grid->setRowStretch(1,0); + grid->addWidget(mSpatialPlot,2,0,1,4); + grid->addWidget(MakeTracePlot(),3,0,1,4); + setLayout(grid); + +} + + + diff --git a/Analysis/DataViewer/BfMaskTab.h b/Analysis/DataViewer/BfMaskTab.h new file mode 100644 index 00000000..abda9348 --- /dev/null +++ b/Analysis/DataViewer/BfMaskTab.h @@ -0,0 +1,27 @@ +/* Copyright (C) 2016 Ion Torrent Systems, Inc. All Rights Reserved */ +#ifndef BFMASKTAB_H +#define BFMASKTAB_H + +#include +#include +#include "dialog.h" +#include "modeltab.h" +#include "BfMaskSpatial.h" + +class Dialog; + +class BfMaskTab : public ModelTab +{ + Q_OBJECT +public: + explicit BfMaskTab(QString mName, Dialog *_mParent, QWidget *parent = 0); + +signals: + +public slots: + +private: + BfMaskSpatial *mBfSpatialPlot; +}; + +#endif // BFMASKTAB_H diff --git a/Analysis/DataViewer/DataViewer.pro b/Analysis/DataViewer/DataViewer.pro new file mode 100644 index 00000000..4a762da2 --- /dev/null +++ b/Analysis/DataViewer/DataViewer.pro @@ -0,0 +1,64 @@ +QT += widgets core gui printsupport + +CONFIG += c++11 +INCLUDEPATH += /usr/include/hdf5/serial/ ../ ../Util/ ../Mask/ ../TsInputUtil/ ../Wells/ ../Image/ ../../build/bamtools-2.4.0.20150702+git15eadb925f-install/include/bamtools/ ../VariantCaller/Bookkeeping/ + +QMAKE_CXXFLAGS += -mavx + +HEADERS = \ + dialog.h \ + AlignmentTab.h \ + AlignmentSpatial.h \ + BfMaskTab.h \ + RawTab.h \ + qcustomplot.h \ + SpatialPlot.h \ + RawSpatial.h \ + WellsTab.h \ + WellsSpatial.h \ + BfMaskSpatial.h \ + NoiseTab.h \ + NoiseSpatial.h \ + GainSpatial.h \ + GainTab.h \ + modeltab.h + + +SOURCES = main.cpp \ + dialog.cpp \ + AlignmentTab.cpp \ + BfMaskTab.cpp \ + RawTab.cpp \ + qcustomplot.cpp \ + SpatialPlot.cpp \ + RawSpatial.cpp \ + WellsTab.cpp \ + WellsSpatial.cpp \ + ../Image/CorrNoiseCorrector.cpp \ + ../Image/ComparatorNoiseCorrector.cpp \ + ../Image/AdvCompr.cpp \ + ../Image/PCACompression.cpp \ + ../Image/deInterlace.cpp \ + ../Image/ChipIdDecoder.cpp \ + ../Wells/RawWells.cpp \ + ../Wells/RawWellsV1.cpp \ + ../Util/IonErr.cpp \ + ../Util/Utils.cpp \ + ../Util/IonH5File.cpp \ + ../LinuxCompat.cpp \ + ../TsInputUtil/IonVersion.cpp \ + BfMaskSpatial.cpp \ + NoiseTab.cpp \ + NoiseSpatial.cpp \ + GainSpatial.cpp \ + GainTab.cpp \ + AlignmentSpatial.cpp \ + ../Calibration/FlowAlignment.cpp \ + modeltab.cpp + + +unix:!mac:!vxworks:!integrity:!haiku:LIBS += -lm -lhdf5_serial "../../build/bamtools-2.4.0.20150702+git15eadb925f-install/lib/bamtools/libbamtools.a" -lz + +# install +#target.path = DataViewer +#INSTALLS += target diff --git a/Analysis/DataViewer/DataViewer.pro.user b/Analysis/DataViewer/DataViewer.pro.user new file mode 100644 index 00000000..5f0214e6 --- /dev/null +++ b/Analysis/DataViewer/DataViewer.pro.user @@ -0,0 +1,271 @@ + + + + + + EnvironmentId + {ebf36358-ae97-47f9-9618-542ce8c93f84} + + + ProjectExplorer.Project.ActiveTarget + 0 + + + ProjectExplorer.Project.EditorSettings + + true + false + true + + Cpp + + CppGlobal + + + + QmlJS + + QmlJSGlobal + + + 2 + UTF-8 + false + 4 + false + 80 + true + true + 1 + true + false + 0 + true + 0 + 8 + true + 1 + true + true + true + false + + + + ProjectExplorer.Project.PluginSettings + + + + ProjectExplorer.Project.Target.0 + + Desktop + Desktop + {9cc9bd26-12f2-4344-84f8-4f85402018cb} + 0 + 0 + 0 + + /home/ionadmin/dev/torrent-suite/Analysis/build-DataViewer-Desktop-Debug + + + true + qmake + + QtProjectManager.QMakeBuildStep + false + true + + false + false + false + + + true + Make + + Qt4ProjectManager.MakeStep + + -w + -r + + false + + + + 2 + Build + + ProjectExplorer.BuildSteps.Build + + + + true + Make + + Qt4ProjectManager.MakeStep + + -w + -r + + true + clean + + + 1 + Clean + + ProjectExplorer.BuildSteps.Clean + + 2 + false + + Debug + + Qt4ProjectManager.Qt4BuildConfiguration + 2 + true + + + /home/ionadmin/dev/torrent-suite/Analysis/build-DataViewer-Desktop-Release + + + true + qmake + + QtProjectManager.QMakeBuildStep + false + true + + false + false + false + + + true + Make + + Qt4ProjectManager.MakeStep + + -w + -r + + false + + + + 2 + Build + + ProjectExplorer.BuildSteps.Build + + + + true + Make + + Qt4ProjectManager.MakeStep + + -w + -r + + true + clean + + + 1 + Clean + + ProjectExplorer.BuildSteps.Clean + + 2 + false + + Release + + Qt4ProjectManager.Qt4BuildConfiguration + 0 + true + + 2 + + + 0 + Deploy + + ProjectExplorer.BuildSteps.Deploy + + 1 + Deploy locally + + ProjectExplorer.DefaultDeployConfiguration + + 1 + + + + false + false + false + false + true + 0.01 + 10 + true + 1 + 25 + + 1 + true + false + true + valgrind + + 0 + 1 + 2 + 3 + 4 + 5 + 6 + 7 + 8 + 9 + 10 + 11 + 12 + 13 + 14 + + 2 + + DataViewer + + Qt4ProjectManager.Qt4RunConfiguration:/home/ionadmin/dev/torrent-suite/Analysis/DataViewer/DataViewer.pro + + DataViewer.pro + false + false + + 3768 + false + true + false + false + true + + 1 + + + + ProjectExplorer.Project.TargetCount + 1 + + + ProjectExplorer.Project.Updater.FileVersion + 18 + + + Version + 18 + + diff --git a/Analysis/DataViewer/GainSpatial.cpp b/Analysis/DataViewer/GainSpatial.cpp new file mode 100644 index 00000000..acb9bc71 --- /dev/null +++ b/Analysis/DataViewer/GainSpatial.cpp @@ -0,0 +1,102 @@ +/* Copyright (C) 2016 Ion Torrent Systems, Inc. All Rights Reserved */ +#include "GainSpatial.h" +#include "RawWells.h" +#include +#include + +using namespace std; + +GainSpatial::GainSpatial(QWidget *parent): SpatialPlot(parent) +{ +} + +void GainSpatial::addMask(uint mask) +{ + mMask |= mask; + render(); +} + +void GainSpatial::RemoveMask(uint mask) +{ + mMask &= ~mask; + render(); +} + +void GainSpatial::doConvert(int &loading) +{ + if(fname == ""){ + free(out); + out=gainVals=NULL; + last_fname = _fname; + traces_len=0; + } + if (last_fname != _fname){ + loading=1; + // load a new dat file + printf("triggered %s %s\n",last_fname.toLatin1().data(),_fname.toLatin1().data()); + last_fname = _fname; + if(out) + free(out); + out=gainVals=NULL; + + QByteArray ba = fname.toLatin1(); + char * fn = ba.data(); + + { + //Note, fillMask is optional argument w/ default of true. + FILE *in = fopen ( fn, "rb" ); + if(in){ + int magic=0, version=0, elements_read=0; + int lrows=0,lcols=0; + + elements_read = fread ( &magic, sizeof ( magic ), 1, in ); + if( (elements_read == 1) && ((uint)magic == 0xFF115E3A)){ + elements_read = fread ( &version, sizeof ( version ), 1, in ); + if( elements_read == 1 ){ + elements_read = fread ( &lrows, sizeof ( rows ), 1, in ); + if( elements_read == 1 ){ + elements_read = fread ( &lcols, sizeof ( cols ), 1, in ); + if( elements_read == 1 ){ + int nbytes = sizeof(out[0])*rows*cols; + out = gainVals = (float *)malloc(nbytes); + elements_read = fread ( out, nbytes, 1, in ); + if( elements_read == 1 ){ + // success.. + if((cols ==0 || cols == lcols) && + (rows == 0 || rows == lrows)){ + cols = lcols; + rows = lrows; + } + else{ + free(out); + out=NULL; + } + } + } + } + } + } + fclose ( in ); + } + } + traces_len=2; + for(int i=0;i +#include "SpatialPlot.h" +#include "qcustomplot.h" + +class GainSpatial : public SpatialPlot +{ +public: + GainSpatial(QWidget *parent); + void doConvert(int &loading); + void addMask(uint mask); + void RemoveMask(uint mask); + +protected: + virtual float Get_Data(int idx, int y, int x); + +private: + float *out=NULL; + uint mMask=0; +}; + +#endif // GAINSPATIAL_H diff --git a/Analysis/DataViewer/GainTab.cpp b/Analysis/DataViewer/GainTab.cpp new file mode 100644 index 00000000..35dab684 --- /dev/null +++ b/Analysis/DataViewer/GainTab.cpp @@ -0,0 +1,27 @@ +/* Copyright (C) 2016 Ion Torrent Systems, Inc. All Rights Reserved */ +#include "GainTab.h" +#include "Mask.h" + + +static const maskCheckBox_t mcbt1[] = { +}; + + +GainTab::GainTab(QString _mName, Dialog *_mParent, QWidget *parent) + : ModelTab(_mName, _mParent,parent) +{ + mGainSpatialPlot = new GainSpatial(this); + mSpatialPlot = mGainSpatialPlot; + + QGridLayout *grid = new QGridLayout; + grid->addWidget(MakeSlider(""),0,0); + grid->addWidget(MakeTraceCB(),1,0); + grid->addWidget(MakeList(mcbt1,sizeof(mcbt1)/sizeof(mcbt1[0])),0,1,2,1); + grid->setRowStretch(0,0); + grid->setRowStretch(1,0); + grid->addWidget(mSpatialPlot,2,0,1,4); + grid->addWidget(MakeTracePlot(),3,0,1,4); + setLayout(grid); +} + + diff --git a/Analysis/DataViewer/GainTab.h b/Analysis/DataViewer/GainTab.h new file mode 100644 index 00000000..33562a9c --- /dev/null +++ b/Analysis/DataViewer/GainTab.h @@ -0,0 +1,25 @@ +/* Copyright (C) 2016 Ion Torrent Systems, Inc. All Rights Reserved */ +#ifndef GAINTAB_H +#define GAINTAB_H + +#include +#include +#include "dialog.h" +#include "GainSpatial.h" +#include "modeltab.h" + +class Dialog; + +class GainTab : public ModelTab +{ + Q_OBJECT +public: + explicit GainTab(QString _mName, Dialog *_mParent, QWidget *parent = 0); + +public slots: + +private: + GainSpatial *mGainSpatialPlot=NULL; +}; + +#endif // GAINTAB_H diff --git a/Analysis/DataViewer/MainForm.ui.qml b/Analysis/DataViewer/MainForm.ui.qml new file mode 100644 index 00000000..f2cd99ee --- /dev/null +++ b/Analysis/DataViewer/MainForm.ui.qml @@ -0,0 +1,29 @@ +import QtQuick 2.7 + +Rectangle { + property alias mouseArea: mouseArea + property alias textEdit: textEdit + + width: 360 + height: 360 + + MouseArea { + id: mouseArea + anchors.fill: parent + } + + TextEdit { + id: textEdit + text: qsTr("Enter some text...") + verticalAlignment: Text.AlignVCenter + anchors.top: parent.top + anchors.horizontalCenter: parent.horizontalCenter + anchors.topMargin: 20 + Rectangle { + anchors.fill: parent + anchors.margins: -10 + color: "transparent" + border.width: 1 + } + } +} diff --git a/Analysis/DataViewer/NoiseSpatial.cpp b/Analysis/DataViewer/NoiseSpatial.cpp new file mode 100644 index 00000000..342ebea0 --- /dev/null +++ b/Analysis/DataViewer/NoiseSpatial.cpp @@ -0,0 +1,100 @@ +/* Copyright (C) 2016 Ion Torrent Systems, Inc. All Rights Reserved */ +#include "NoiseSpatial.h" +#include "RawWells.h" +#include "dialog.h" +#include +#include + +using namespace std; + +NoiseSpatial::NoiseSpatial(QWidget *parent): SpatialPlot(parent) +{ +} + +void NoiseSpatial::addMask(uint mask) +{ + mMask |= mask; + render(); +} + +void NoiseSpatial::RemoveMask(uint mask) +{ + mMask &= ~mask; + render(); +} + +void NoiseSpatial::doConvert(int &loading) +{ + if(fname == ""){ + free(out); + out=NULL; + last_fname = _fname; + traces_len=0; + } + if (last_fname != _fname){ + loading=1; + // load a new dat file + printf("triggered %s %s\n",last_fname.toLatin1().data(),_fname.toLatin1().data()); + last_fname = _fname; + if(out) + free(out); + out=NULL; + + QByteArray ba = fname.toLatin1(); + char * fn = ba.data(); + + { + //Note, fillMask is optional argument w/ default of true. + FILE *in = fopen ( fn, "rb" ); + if(in){ + uint magic=0, version=0, elements_read=0; + uint lrows=0,lcols=0; + uint RawExpmt_Y=Block_Y; + uint RawExpmt_X=Block_X; + + elements_read = fread ( &magic, sizeof ( magic ), 1, in ); + if( (elements_read == 1) && (magic == 0xFF115E3A)){ + elements_read = fread ( &version, sizeof ( version ), 1, in ); + if( elements_read == 1 ){ + elements_read = fread ( &lcols, sizeof ( lcols ), 1, in ); + if( elements_read == 1 ){ + elements_read = fread ( &lrows, sizeof ( lrows ), 1, in ); + if( elements_read == 1 ){ + + // we're ready to read in the data... + int nbytes=sizeof(out[0])*rows*cols; + out = (uint16_t *)(uint16_t *)malloc(nbytes); + + fseek(in, (RawExpmt_Y*lcols + RawExpmt_X)*sizeof(out[0]),SEEK_CUR);// seek to the right part of the row + for(int r=0;r +#include "SpatialPlot.h" +#include "qcustomplot.h" + +class Dialog; + +class NoiseSpatial : public SpatialPlot +{ +public: + NoiseSpatial(QWidget *parent); + + void doConvert(int &loading); + void addMask(uint mask); + void RemoveMask(uint mask); + float Get_Data(int idx, int y, int x); + +private: + uint16_t *out=NULL; + uint mMask=0; +}; + +#endif // NOISESPATIAL_H diff --git a/Analysis/DataViewer/NoiseTab.cpp b/Analysis/DataViewer/NoiseTab.cpp new file mode 100644 index 00000000..9bc4281a --- /dev/null +++ b/Analysis/DataViewer/NoiseTab.cpp @@ -0,0 +1,24 @@ +/* Copyright (C) 2016 Ion Torrent Systems, Inc. All Rights Reserved */ +#include "NoiseTab.h" +#include "Mask.h" + +static const maskCheckBox_t mcbt1[] = {}; + +NoiseTab::NoiseTab(QString _mName, Dialog *_mParent, QWidget *parent) + : ModelTab(_mName, _mParent, parent) +{ + + mSpatialPlot = new NoiseSpatial(this); + + QGridLayout *grid = new QGridLayout; + grid->addWidget(MakeSlider(""),0,0); + grid->addWidget(MakeTraceCB(),1,0); + grid->addWidget(MakeList(mcbt1,sizeof(mcbt1)/sizeof(mcbt1[0])),0,1,2,1); + grid->setRowStretch(0,0); + grid->setRowStretch(1,0); + grid->addWidget(mSpatialPlot,2,0,1,4); + grid->addWidget(MakeTracePlot(),3,0,1,4); + setLayout(grid); + +} + diff --git a/Analysis/DataViewer/NoiseTab.h b/Analysis/DataViewer/NoiseTab.h new file mode 100644 index 00000000..96bc766d --- /dev/null +++ b/Analysis/DataViewer/NoiseTab.h @@ -0,0 +1,25 @@ +/* Copyright (C) 2016 Ion Torrent Systems, Inc. All Rights Reserved */ +#ifndef NOISETAB_H +#define NOISETAB_H + +#include +#include +#include "NoiseSpatial.h" +#include "modeltab.h" + +class Dialog; + +class NoiseTab : public ModelTab +{ + Q_OBJECT +public: + explicit NoiseTab(QString _mName, Dialog *_mParent, QWidget *parent = 0); + +signals: + +public slots: + +private: +}; + +#endif // NOISETAB_H diff --git a/Analysis/DataViewer/README b/Analysis/DataViewer/README new file mode 100644 index 00000000..f69a7a83 --- /dev/null +++ b/Analysis/DataViewer/README @@ -0,0 +1,4 @@ +sudo apt-get install qtcreator +sudo apt-get install qtbase5-examples qtbase5-doc-html qt5-doc qt5-doc-html +sudo apt-get install qt5-default + diff --git a/Analysis/DataViewer/RawSpatial.cpp b/Analysis/DataViewer/RawSpatial.cpp new file mode 100644 index 00000000..31f2cbfc --- /dev/null +++ b/Analysis/DataViewer/RawSpatial.cpp @@ -0,0 +1,391 @@ +/* Copyright (C) 2016 Ion Torrent Systems, Inc. All Rights Reserved */ +#include "RawSpatial.h" +#include "deInterlace.h" +#include "CorrNoiseCorrector.h" +#include "ComparatorNoiseCorrector.h" +#include "ChipIdDecoder.h" +#include "AdvCompr.h" +#include "Mask.h" + + +RawSpatial::RawSpatial(QWidget *parent): SpatialPlot(parent) +{ +} + +void RawSpatial::SetOption(QString txt, int state) +{ + qDebug() << __PRETTY_FUNCTION__ << ": " << txt << state; + + if(txt == "Zero traces") + zeroState = state; + else if(txt == "Rmv Row Noise") + RowNoiseState = state; + else if(txt == "Rmv Col Noise") + ColNoiseState = state; + else if(txt == "Gain Correct") + gainCorrState = state; + else if(txt == "NeighborSubtract") + neighborState = state; + else if(txt == "RefSubtract") + RefState = state; + else if(txt == "EmptySubtract") + EmptySubState = state; + else if(txt == "Column Flicker") + ColFlState = state; + else if(txt == "Adv Compression") + AdvcState = state; + else if(txt == "Show Masked") + MaskReads = state; + else if(txt == "StdDev") + stdState = state; + + render(); +} + + +void RawSpatial::doConvert(int &loading) +{ + + if((rowNoiseRemoved && !RowNoiseState) || + (colNoiseRemoved && !ColNoiseState) || + (gainCorrApplied && !gainCorrState) || + (neighborSubApplied && !neighborState) || + (RefSubApplied && !RefState) || + (ColFlApplied && !ColFlState) || + (EmptySubApplied && !EmptySubState) || + (AdvcApplied && !AdvcState) || + (stdApplied && !stdState)){ + // re-load the image.... + last_fname.clear(); + } + + if(fname == ""){ + free(out); + out=NULL; + last_fname = _fname; + traces_len=0; + } + if (last_fname != _fname){ + // load a new dat file + loading=1; + printf("triggered %s %s\n",last_fname.toLatin1().data(),_fname.toLatin1().data()); + last_fname = _fname; + if(out) + free(out); + out=NULL; + + if(timestamps) + free(timestamps); + timestamps=NULL; + + if(gainImage) + free(gainImage); + gainImage=NULL; + + + QByteArray ba = fname.toLatin1(); + char * fn = ba.data(); + printf("about to call deInterlace with %s\n",fn); + deInterlace_c (fn, &out, ×tamps, + &rows, &cols, &frames, &uncompFrames, + 0, 0, + 0, 0, 0, 0, 0, &imageState ); + printf("\ndone\n\n"); + rowNoiseRemoved=0; + colNoiseRemoved=0; + gainCorrApplied=0; + neighborSubApplied=0; + RefSubApplied=0; + EmptySubApplied=0; + ColFlApplied=0; + AdvcApplied=0; + stdApplied=0; + if(endX==0){ + startX=0; + startY=0; + endX=cols; + endY=rows; + } + for(uint i=0;i<(uint)frames && i < (sizeof(traces_ts)/sizeof(traces_ts[0]));i++) + traces_ts[i]=((double)timestamps[i])/1000.0f; + traces_len=frames; + loading=0; + + if(out){ + if(mMask){ + free(mMask); + mMask=NULL; + } + mMask = (char *)malloc(rows*cols); + memset(mMask,0,rows*cols); + } + + } + + if(out){ + + + //we have loaded a image into memory.. + if(!rowNoiseRemoved && RowNoiseState){ + ChipIdDecoder::SetGlobalChipId("p2.2.2"); + CorrNoiseCorrector rnc; + /*double RowNoise = */rnc.CorrectCorrNoise(out, rows, cols, frames, 1, 0, 0,0,-1,0); + rowNoiseRemoved=1; + } + if(!colNoiseRemoved && ColNoiseState){ + ChipIdDecoder::SetGlobalChipId("p2.2.2"); + CorrNoiseCorrector rnc; + /*double ColNoise = */rnc.CorrectCorrNoise(out, rows, cols, frames, 2, 0, 0,0,-1,0); + colNoiseRemoved=1; + } + if(!gainCorrApplied && gainCorrState && gainVals){ + GainCorrect(out,rows,cols,frames); + gainCorrApplied=1; + } + if(!neighborSubApplied && neighborState){ + // do neighbor subtraction + NeighborSubtract(out, rows, cols, frames,NULL,0); + neighborSubApplied=1; + } + if(!RefSubApplied && RefState && refMask){ + // do neighbor subtraction + NeighborSubtract(out, rows, cols, frames, refMask,1); + RefSubApplied=1; + } + if(!EmptySubApplied && EmptySubState && refMask){ + // do neighbor subtraction + NeighborSubtract(out, rows, cols, frames, refMask,0); + EmptySubApplied=1; + } + if(!ColFlApplied && ColFlState){ + // do column flicker correction + ComparatorNoiseCorrector cnc; + cnc.CorrectComparatorNoise(out,rows,cols,frames,NULL,0,1,0); + ColFlApplied=1; + } + if(!AdvcApplied && AdvcState){ + ChipIdDecoder::SetGlobalChipId("p2.2.2"); + AdvCompr advc(-1/*ExtFd*/, out, cols, rows, frames, uncompFrames, + timestamps, timestamps, 1, (char *)"",(char *)"",15,1); + advc.Compress(-1,0,0,0); + AdvcApplied=1; + } + if(!stdApplied && stdState){ + // take spatial standard deviation of the pixel offsets + TakeStdDev(); + stdApplied=1; + } + + fflush(stdout); + UpdateTraceData(); + } +} + +void RawSpatial::TakeStdDev() +{ + int spanW=2; + int spanH=2; + + // make a mask of the first frame.. + for(int r=0;r= rows) + endy=rows; + for(int c=0;c= cols) + endx=cols; + + float localSum=0; + int localSumCnt=0; + for(int ry=starty;ry0){ + lsum[y*w+x] =lsum[(y-1)*w+x] + rptr[y*w+x]; + lsumNum[y*w+x]=lsumNum[(y-1)*w+x] + 1; + } + else{ + lsum[y*w+x]=rptr[x]; + lsumNum[y*w+x]=1; + } + } + else{ + if(y>0){ + lsum[y*w+x]=lsum[(y-1)*w+x]; + lsumNum[y*w+x]=lsumNum[(y-1)*w+x]; + } + else + lsum[y*w+x]=0; + } + for(x=1;x0){ + lsum[y*w+x]=lsum[(y-1)*w+x] + lsum[y*w+x-1] - lsum[(y-1)*w+x-1] + rptr[y*w+x]; + lsumNum[y*w+x]=lsumNum[(y-1)*w+x] + lsumNum[y*w+x-1] - lsumNum[(y-1)*w+x-1] + 1; + } + else{ + lsum[y*w+x]= lsum[y*w+x-1] + rptr[y*w+x]; + lsumNum[y*w+x]= lsumNum[y*w+x-1] + 1; + } } + else{ + if(y>0){ + lsum[y*w+x]=lsum[(y-1)*w+x] + lsum[y*w+x-1] - lsum[(y-1)*w+x-1]; + lsumNum[y*w+x]=lsumNum[(y-1)*w+x] + lsumNum[y*w+x-1] - lsumNum[(y-1)*w+x-1]; + } + else{ + lsum[y*w+x]= lsum[y*w+x-1]; + lsumNum[y*w+x]= lsumNum[y*w+x-1]; + } + } + } + } + // save the mean for this frame +// if(saved_mean) +// saved_mean[frame]=(float)lsum[(h-1)*w+(w-1)]/(float)(w*h); + // now, neighbor subtract each well + for(int y=0;y= w) + end_x = w-1; + if(start_y < 0) + start_y=0; + if(end_y >= h) + end_y=h-1; + int end=end_y*w+end_x; + int start=start_y*w+start_x; + int diag1=end_y*w+start_x; + int diag2=start_y*w+end_x; + +// if(y < 12 && x < 12) +// printf(" (%d/%d = %d)",(int)(lsum[end_y][end_x]-lsum[start_y][start_x]),((end_y-start_y+1)*(end_x-start_x+1)), +// (int)((lsum[end_y][end_x]-lsum[start_y][start_x])/((end_y-start_y+1)*(end_x-start_x+1)) + 8192)); + int64_t avg = lsum[end]+lsum[start]-lsum[diag1]-lsum[diag2]; + int64_t num=lsumNum[end]+lsumNum[start]-lsumNum[diag1]-lsumNum[diag2]; + if(num){ + avg /= num; + rptr[y*w+x] = rptr[y*w+x] - (short int)avg/* + 8192*/; + } + else{ +// printf("Problems here %d/%d\n",y,x); + } + } + } + } + free(lsum); + free(lsumNum); +} + + + + diff --git a/Analysis/DataViewer/RawSpatial.h b/Analysis/DataViewer/RawSpatial.h new file mode 100644 index 00000000..afa60483 --- /dev/null +++ b/Analysis/DataViewer/RawSpatial.h @@ -0,0 +1,59 @@ +/* Copyright (C) 2016 Ion Torrent Systems, Inc. All Rights Reserved */ +#ifndef RAWSPATIAL_H +#define RAWSPATIAL_H + +#include +#include "SpatialPlot.h" +#include "qcustomplot.h" + +class RawSpatial : public SpatialPlot +{ +public: + RawSpatial(QWidget *parent); + void doConvert(int &loading); + void SetOption(QString option, int state); + +protected: + virtual float Get_Data(int frame, int y, int x); + + +private: + void NeighborSubtract(short int *raw, int h, int w, int npts, uint16_t *mask, int ref); + void GainCorrect(short int *raw, int h, int w, int npts); + void TakeStdDev(); + + int zeroState=0; + int RowNoiseState=0; + int ColNoiseState=0; + int gainCorrState=0; + int neighborState=0; + int RefState=0; + int EmptySubState=0; + int ColFlState=0; + int AdvcState=0; + int stdState=0; + int MaskReads=0; + + // thread copy of app specific stuff + + // thread specific stuff + short *out=NULL; + int *timestamps=NULL; + int uncompFrames=0; + int imageState=0; + short *gainImage=NULL; + + + int rowNoiseRemoved=0; + int colNoiseRemoved=0; + int gainCorrApplied=0; + int neighborSubApplied=0; + int RefSubApplied=0; + int EmptySubApplied=0; + int ColFlApplied=0; + int AdvcApplied=0; + int stdApplied=0; + +}; + +#endif // RAWSPATIAL_H diff --git a/Analysis/DataViewer/RawTab.cpp b/Analysis/DataViewer/RawTab.cpp new file mode 100644 index 00000000..a8c5d72d --- /dev/null +++ b/Analysis/DataViewer/RawTab.cpp @@ -0,0 +1,43 @@ +/* Copyright (C) 2016 Ion Torrent Systems, Inc. All Rights Reserved */ +#include "RawTab.h" +#include "qcustomplot.h" +#include "deInterlace.h" +#include "CorrNoiseCorrector.h" + +static const maskCheckBox_t mcbt1[] = { + {"Zero traces",0,Qt::Unchecked}, + {"Rmv Row Noise",1,Qt::Unchecked}, + {"Rmv Col Noise",2,Qt::Unchecked}, + {"Gain Correct",3,Qt::Unchecked}, + {"NeighborSubtract",4,Qt::Unchecked}, + {"RefSubtract",5,Qt::Unchecked}, +// {"EmptySubtract",6,Qt::Unchecked}, + {"Column Flicker",6,Qt::Unchecked}, + {"Adv Compression",7,Qt::Unchecked}, + {"Show Masked",8,Qt::Unchecked}, + {"StdDev",9,Qt::Unchecked}, +}; + +RawTab::RawTab(QString _mName, Dialog *_mParent, QWidget *parent) + : ModelTab(_mName, _mParent, parent) +{ + mSpatialPlot = new RawSpatial(this); + + QGridLayout *grid = new QGridLayout; + grid->addWidget(MakeSlider("frames"),0,0); + grid->addWidget(MakeTraceCB(),1,0); + grid->addWidget(MakeList(mcbt1,sizeof(mcbt1)/sizeof(mcbt1[0])),0,1,2,1); + grid->setRowStretch(0,0); + grid->setRowStretch(1,0); + grid->addWidget(mSpatialPlot,2,0,1,4); + grid->addWidget(MakeTracePlot(),3,0,1,4); + + // add compression points to graph + for(int i=0;igraph(i)->setScatterStyle(QCPScatterStyle::ssCircle); + } + + setLayout(grid); +} + + diff --git a/Analysis/DataViewer/RawTab.h b/Analysis/DataViewer/RawTab.h new file mode 100644 index 00000000..1ff8df80 --- /dev/null +++ b/Analysis/DataViewer/RawTab.h @@ -0,0 +1,26 @@ +/* Copyright (C) 2016 Ion Torrent Systems, Inc. All Rights Reserved */ +#ifndef RAWTAB_H +#define RAWTAB_H + +#include +#include +#include "dialog.h" +#include "RawSpatial.h" +#include "qcustomplot.h" +#include "modeltab.h" + +class Dialog; + +class RawTab : public ModelTab +{ + Q_OBJECT + +public: + explicit RawTab(QString _mName, Dialog *_mParent, QWidget *parent = 0); + +public slots: + +private: +}; + +#endif // RAWTAB_H diff --git a/Analysis/DataViewer/SpatialPlot.cpp b/Analysis/DataViewer/SpatialPlot.cpp new file mode 100644 index 00000000..0e0737bb --- /dev/null +++ b/Analysis/DataViewer/SpatialPlot.cpp @@ -0,0 +1,1002 @@ +/* Copyright (C) 2016 Ion Torrent Systems, Inc. All Rights Reserved */ + +#include +#include + +#include + +#include "SpatialPlot.h" + + +//! [0] +const double ZoomInFactor = 0.8; +const double ZoomOutFactor = 1 / ZoomInFactor; +const int ScrollStep = 20; +//! [0] + +// window variables +int SpatialPlot::startX=0; +int SpatialPlot::endX=0; +int SpatialPlot::startY=0; +int SpatialPlot::endY=0; +int SpatialPlot::frame=0; +int SpatialPlot::flow=0; + +// image variables +int SpatialPlot::rows=0; +int SpatialPlot::cols=0; +int SpatialPlot::frames=0; +int SpatialPlot::flows=0; + +int SpatialPlot::Block_X=0; +int SpatialPlot::Block_Y=0; +char *SpatialPlot::mMask = NULL; + +TraceDescr_t SpatialPlot::traces[MAX_NUM_TRACES] = {{0,0}}; +int SpatialPlot::traces_curSelection=0; +int SpatialPlot::GblTracesVer=0; +uint16_t *SpatialPlot::refMask=NULL; //from BfMaskTab +float *SpatialPlot::gainVals=NULL; //from GainTab + + +//! [1] +SpatialPlot::SpatialPlot(QWidget *parent) + :QWidget(parent) +{ + + connect(&thread, SIGNAL(renderedImage(QImage)), this, SLOT(updatePixmap(QImage))); + + setWindowTitle(tr("SpatialPlot")); +#ifndef QT_NO_CURSOR + setCursor(Qt::CrossCursor); +#endif + resize(550, 400); + setMouseTracking( true ); + setFocusPolicy(Qt::WheelFocus); + + for (int i = 0; i < ColormapSize; ++i) + colormap[i] = rgbFromWaveLength((375.0) + (i * 400.0 / ColormapSize)); + + colormap[0]=0; // make the background black + + thread.SetParent(this); + + memset(traces,0,sizeof(traces)); + for(int trc=0;trc=0) && (traces[trc].y > 0)) + { + for(int idx=0;idx=0) && (traces[trc].y > 0)) + { + QVector x(traces_len); + QVector y(traces_len); + + for (int i=0; i ymax) + ymax = y[i]; + } + + + // create graph and assign data to it: + _mTracePlot->graph(trc)->setData(x, y); + QString name="Y" + QString::number(traces[trc].y) + "_X" + QString::number(traces[trc].x); + _mTracePlot->graph(trc)->setName(name); + _mTracePlotSet[trc]=1; + // set axes ranges, so we see all data: + } + else{ + if(_mTracePlotSet[trc]){ + QString name=""; + _mTracePlot->graph(trc)->setName(name); + _mTracePlotSet[trc]=0; + QVector x(0); + QVector y(0); + _mTracePlot->graph(trc)->setData(x, y); + } + } + } + + { + // add frame/flow line + QVector x(2); + QVector y(2); + x[0] = x[1] = (double)traces_ts[flow_based?flow:frame]; + y[0] = ymin; + y[1] = ymax; + _mTracePlot->graph(MAX_NUM_TRACES)->setData(x, y); + _mTracePlot->graph(MAX_NUM_TRACES)->setName(""); + _mTracePlot->graph(MAX_NUM_TRACES)->setPen(QPen(Qt::DashLine)); + + } + _mTraceRange=ymax-ymin; + _mTracePlot->xAxis->setRange(xmin, xmax); + _mTracePlot->yAxis->setRange(ymin, ymax); + _mTracePlot->replot(); + + } + +} + + +void SpatialPlot::setTracePlot(QCustomPlot *mtracePlot, int _flow_based) +{ + _mTracePlot = mtracePlot; + flow_based=_flow_based; + + double xmin =-1; // set defaults + double xmax = 1; + double ymin = 0; + double ymax = 1; + + // create graph and assign data to it: + for(int i=0;i<(MAX_NUM_TRACES+1);i++){ + mtracePlot->addGraph(); + + if(i < MAX_NUM_TRACES){ + QPen qp; + + uint xcolorIdx= i*ColormapSize/MAX_NUM_TRACES; + uint xcolor=colormap[xcolorIdx % ColormapSize]; + + qp.setColor(QColor(qRed(xcolor),qGreen(xcolor),qBlue(xcolor))); + mtracePlot->graph(i)->setPen(qp); + } + } + + mtracePlot->setInteractions(QCP::iRangeZoom | QCP::iSelectPlottables | QCP::iSelectLegend | QCP::iRangeDrag); // allow selection of graphs via mouse click + // give the axes some labels: + mtracePlot->xAxis->setLabel("x"); + mtracePlot->yAxis->setLabel("y"); + // set axes ranges, so we see all data: + mtracePlot->xAxis->setRange(xmin, xmax); + mtracePlot->yAxis->setRange(ymin, ymax); + mtracePlot->legend->setVisible(true); + //mtracePlot->legend->setAlignment(Qt::AlignTop); + mtracePlot->replot(); + + connect(mtracePlot, SIGNAL(mouseMove(QMouseEvent*)), this,SLOT(showPointToolTip_traces(QMouseEvent*))); + connect(mtracePlot, SIGNAL(mouseDoubleClick(QMouseEvent*)), this,SLOT(mouseDoubleClickEvent_traces(QMouseEvent*))); +} + +void SpatialPlot::showPointToolTip(QMouseEvent *event) +{ + + int x = _mTracePlot->xAxis->pixelToCoord(event->pos().x()); + int y = _mTracePlot->yAxis->pixelToCoord(event->pos().y()); + + _mTracePlot->setToolTip(QString("%1 , %2").arg(x).arg(y)); + + +} + + +void SpatialPlot::Save(QTextStream &strm) +{ + // create graph and assign data to it: + + for(int i=0;i<(MAX_NUM_TRACES);i++){ + + if((traces[i].x >=0) && (traces[i].y > 0)) + { + for(int idx=0;idx<(flow_based?flows:frames);idx++){ + strm << traces_ts[idx] << "," << traces_Val[i][idx] << " "; + } + strm << endl; + } + } +} + +void SpatialPlot::DoubleClick_traces(int x, int y, int ts) +{ + // .... override me ...... + (void)x; + (void)y; + (void)ts; +} + +void SpatialPlot::mouseDoubleClickEvent_traces(QMouseEvent *event) +{ + float x = _mTracePlot->xAxis->pixelToCoord(event->pos().x()); + float y = _mTracePlot->yAxis->pixelToCoord(event->pos().y()); + qDebug() << __PRETTY_FUNCTION__ << ": X" << x << "_Y" << y; + + + // figure out which frame we are looking at... + int min_ts=0; + for(int ts=0;ts=0) && (traces[i].y > 0)) + { + if(std::abs(y - traces_Val[i][min_ts]) < std::abs(y - traces_Val[min_val_idx][min_ts])) + min_val_idx = i; + } + } + + // we now have min_ts and min_val_idx.... + if(std::abs(y-traces_Val[min_val_idx][min_ts]) < (3.0*_mTraceRange/100.0)){ + //_mTracePlot->setToolTip(QString("Y%1_X%2: (%3,%4)").arg(traces[min_val_idx].y).arg(traces[min_val_idx].x).arg(traces_ts[min_ts]).arg(traces_Val[min_val_idx][min_ts])); + DoubleClick_traces(traces[min_val_idx].x,traces[min_val_idx].y,traces_Val[min_val_idx][min_ts]); + } +} + +void SpatialPlot::showPointToolTip_traces(QMouseEvent *event) +{ + + float x = _mTracePlot->xAxis->pixelToCoord(event->pos().x()); + float y = _mTracePlot->yAxis->pixelToCoord(event->pos().y()); + + + // figure out which frame we are looking at... + int min_ts=0; + for(int ts=0;ts=0) && (traces[i].y > 0)) + { + if(std::abs(y - traces_Val[i][min_ts]) < std::abs(y - traces_Val[min_val_idx][min_ts])) + min_val_idx = i; + } + } + + // we now have min_ts and min_val_idx.... + if(std::abs(y-traces_Val[min_val_idx][min_ts]) < (3.0*_mTraceRange/100.0)){ + _mTracePlot->setToolTip(QString("Y%1_X%2: (%3,%4)").arg(traces[min_val_idx].y).arg(traces[min_val_idx].x).arg(traces_ts[min_ts]).arg(traces_Val[min_val_idx][min_ts])); + } +// else{ +// _mTracePlot->setToolTip(""); +// } +} + + +void SpatialPlot::mouseDoubleClickEvent(QMouseEvent *event) +{ + int oobx=0; + int ooby=0; + int ax = GetAX(event->x(),width(),oobx); + int ay = GetAY(event->y(),height(),ooby); + int oob=oobx|ooby; + + DoubleClick(oob?-1:ax,oob?-1:ay); + render(); + printf("%s: %d/%d %d/%d\n",__FUNCTION__,event->x(),event->y(),ax,ay); + initialHints=0; + fflush(stdout); +} +void SpatialPlot::mousePressEvent(QMouseEvent *event){ + LastPressX = event->x(); + LastPressY = event->y(); + printf("%s: %d/%d\n",__FUNCTION__,LastPressX,LastPressY); + fflush(stdout); +} + +void SpatialPlot::mouseReleaseEvent(QMouseEvent *event){ + printf("%s: %d/%d last=%d/%d\n",__FUNCTION__,event->x(),event->y(),LastPressX,LastPressY); + fflush(stdout); + if((LastPressX == event->x()) && + (LastPressY == event->y())){ + // this was a click event.. + printf("%s: calling DoubleClick()\n",__FUNCTION__); + mouseDoubleClickEvent(event);//->x(),event->y(),height(),width()); + } +} + + +//! [10] +void SpatialPlot::resizeEvent(QResizeEvent * /* event */) +{ +// render(); +} +//! [10] + +//! [11] +void SpatialPlot::keyPressEvent(QKeyEvent *event) +{ + switch (event->key()) { + case Qt::Key_Plus: + zoom(ZoomInFactor); + break; + case Qt::Key_Minus: + zoom(ZoomOutFactor); + break; + case Qt::Key_Left: + { + //scroll(-ScrollStep, 0); + int trc=traces_curSelection; + if(traces[trc].x > 0){ + traces[trc].x--; + render(); + } + } + break; + case Qt::Key_Right: + { + //scroll(+ScrollStep, 0); + int trc=traces_curSelection; + if((traces[trc].x+1) < cols){ + traces[trc].x++; + render(); + } + } + break; + case Qt::Key_Down: + { + //scroll(0, -ScrollStep); + int trc=traces_curSelection; + if((traces[trc].y) > 0){ + traces[trc].y--; + render(); + } + + } + break; + case Qt::Key_Up: + { + //scroll(0, +ScrollStep); + int trc=traces_curSelection; + if((traces[trc].y+1) < rows){ + traces[trc].y++; + render(); + } + } + break; + default: + QWidget::keyPressEvent(event); + } +} +//! [11] + +#ifndef QT_NO_WHEELEVENT +//! [12] +void SpatialPlot::wheelEvent(QWheelEvent *event) +{ + int numDegrees = event->delta() / 8; + double numSteps = numDegrees / 15.0f; + double zf = pow(ZoomInFactor, numSteps); + + int oob=0; + int x=event->pos().x(); + int y=event->pos().y(); + int ay=GetAY(y,height(),oob); + int ax=GetAX(x,width(),oob); + if(!oob){ + double WeightX = ((double)(ax - startX)) / ((double)(endX - startX)); + double WeightY = ((double)(ay - startY)) / ((double)(endY - startY)); + printf("%s: %lf %d/%d %d/%d %lf/%lf\n",__FUNCTION__,zf,y,x,ay,ax,WeightY,WeightX); + fflush(stdout); + zoom(zf,WeightX,WeightY); + } +} +//! [12] +#endif + +////! [13] +//void SpatialPlot::mousePressEvent(QMouseEvent *event) +//{ +// if (event->button() == Qt::LeftButton) +// lastDragPos = event->pos(); +//} +////! [13] + +//! [14] +void SpatialPlot::mouseMoveEvent(QMouseEvent *event) +{ + if (event->buttons() & Qt::LeftButton && lastDragPos.x()) { + //int width = endX-startX; + QPoint diff = event->pos() - lastDragPos; + int diffX = (diff.x() * (endX-startX)/width()) ; + int diffY = (diff.y() * (endY-startY)/height()) ; + diffY = -diffY; //it's flipped + printf("%s: %d/%d --> %d/%d\n",__FUNCTION__,event->y(),event->x(),lastDragPos.y(),lastDragPos.x()); + printf("%s: %d/%d/%d/%d --> %d/%d/%d/%d\n",__FUNCTION__,startY,startX,endY,endX,startY-diffY,startX-diffX,endY-diffY,endX-diffX); + fflush(stdout); + if(diffX > startX) + diffX = startX; + if(diffY > startY) + diffY = startY; + if((endX-diffX) > cols) + diffX = endX-cols; + if((endY-diffY) > rows) + diffY = endY-rows; + startX -= diffX; + startY -= diffY; + endX -= diffX; + endY -= diffY; + if(diffX || diffY){ + lastDragPos = event->pos(); + initialHints=0; + } + render(); + } + else{ + lastDragPos = event->pos(); + } +} +//! [14] + +#if 0 +//! [15] +void SpatialPlot::mouseReleaseEvent(QMouseEvent *event) +{ + if (event->button() == Qt::LeftButton) { + pixmapOffset += event->pos() - lastDragPos; + lastDragPos = QPoint(); + + int deltaX = (width() - pixmap.width()) / 2 - pixmapOffset.x(); + int deltaY = (height() - pixmap.height()) / 2 - pixmapOffset.y(); + scroll(deltaX, deltaY); + } +} +//! [15] +#endif +//! [16] +void SpatialPlot::updatePixmap(const QImage &image) +{ + pixmap = QPixmap::fromImage(image); + UpdateTracePlot(); + update(); + +} +//! [16] + +//! [17] +void SpatialPlot::zoom(double zoomFactor, double XWeight, double YWeight) +{ + int diffX = (int)((double)(endX-startX) * (1.0-zoomFactor))/2; + int diffY = (int)((double)(endY-startY) * (1.0-zoomFactor))/2; + if(diffX >= 0 && diffX < 4) + diffX=4; + if(diffY >= 0 && diffY < 4) + diffY=4; + + if(diffX <= 0 && diffX > -4) + diffX=-4; + if(diffY <= 0 && diffY > -4) + diffY=-4; + + int diffXS = (int)((double)diffX * XWeight); + int diffXE = diffX-diffXS; + int diffYS = (int)((double)diffY * YWeight); + int diffYE = diffY-diffYS; + + qDebug() << __FUNCTION__ << ": (" << startY << "/" << startX << ") (" << endY << "/" << endX << ") zf:" << zoomFactor << " diffS(" << diffYS << "/" << diffXS << ") diffE(" << diffYE << "/" << diffXE << ")"; + + startX += diffXS; + startY += diffYS; + endX -= diffYE; + endY -= diffYE; + if(startX < 0) + startX=0; + if(startY<0) + startY=0; + if(endX > cols) + endX=cols; + if(endY > rows) + endY=rows; + initialHints=0; + render(); +} +//! [17] + +//! [18] +void SpatialPlot::scroll(int deltaX, int deltaY) +{ + int diffX = (int)((double)(endX-startX) * (deltaX/width())); + int diffY = (int)((double)(endY-startY) * (deltaY/height())); + startX += diffX; + startY -= diffY; + endX += diffX; + endY -= diffY; + initialHints=0; + update(); +} +//! [18] + +void SpatialPlot::doConvertInt() +{ + doConvert(loading); +// loading=0; +} + +void SpatialPlot::doConvert(int &loading){(void)loading;} + + +void SpatialPlot::copyData() +{ + _fname = fname; + +} + +//! [0] +SpatialPlotThread::SpatialPlotThread(QObject *parent) + : QThread(parent) +{ + restart = false; + abort = false; + +} +void SpatialPlotThread::SetParent(SpatialPlot *_parent) +{ + mParent = _parent; +} + +//! [0] + +//! [1] +SpatialPlotThread::~SpatialPlotThread() +{ + mutex.lock(); + abort = true; + condition.wakeOne(); + mutex.unlock(); + + wait(); +} +//! [1] + +void SpatialPlot::copyLastCoord() +{ + lastStartX = startX; + lastStartY = startY; + lastEndX = endX; + lastEndY = endY; +} + +//! [2] +void SpatialPlotThread::render() +{ + mParent->copyLastCoord(); + QMutexLocker locker(&mutex); + + if (!isRunning()) { + start(LowPriority); + } else { + restart = true; + condition.wakeOne(); + } +} +//! [2] + +void SpatialPlotThread::Abort() +{ + abort=true; + mutex.unlock(); +} + +void SpatialPlotThread::run() +{ + forever { + mutex.lock(); + if(abort) + return; // application is closed + mParent->copyData(); + mutex.unlock(); + + mParent->doConvertInt(); + + QImage *image = mParent->doRender(); + if(image){ + emit renderedImage(*image); + free(image); + } + + mutex.lock(); + if (!restart) + condition.wait(&mutex); + restart = false; + mutex.unlock(); + } +} + +// get data coords from screen coords +int SpatialPlot::GetAY(int y, int Height, int & ooby) +{ + double bh = borderHeight; + double rh = (double)(endY-startY); + double screenh = (double)(Height - bh); + int ay = endY - bh - 1 - (int)(((double)y/*-bh*/) * rh / screenh); + if((y >= screenh)) + ooby=1; + if(ay >= rows || ay < 0) + ooby = 1; + return ay; +} + +// get data coords from screen coords +int SpatialPlot::GetAX(int x, int Width, int & oobx) +{ + int bw = borderWidth+scaleBarWidth+2; + double rw = (double)(endX-startX); + double screenw = (double)(Width-bw); + int ax = startX + (int)((double)(x-borderWidth) * rw/screenw); + if(x < borderWidth || (x > (Width-(scaleBarWidth+2)))) + oobx=1; + if(ax >= cols || ax < 0) + oobx=1; + return ax; +} + +// get screen coords from data coords +int SpatialPlot::GetY(int ay, int Height, int & ooby) +{ + double rh = (double)(endY-startY); + double screenh = (double)(Height - borderHeight); + int y = /*borderHeight +*/ (int)((double)(endY-ay-borderHeight-1) * screenh/rh); + if(y < 0) + ooby=1; + if(y >= Height) + ooby=1; + + return y; +} + +// get screen coords from data coords +int SpatialPlot::GetX(int ax, int Width, int & oobx) +{ + int bw = borderWidth+scaleBarWidth+2; + double rw = (double)(endX-startX); + double screenw = (double)(Width-bw); + ax -= startX; + int x = borderWidth + (int)((double)ax * screenw/rw); + if(x < 0) + oobx=1; + if(x >= Width) + oobx=1; + return x; +} + + +QImage *SpatialPlot::doRender() +{ + int Height = height(); + int Width = width(); + QImage *image = new QImage(Width,Height, QImage::Format_RGB32); + + if(startX < 0) + startX=0; + if(startX > cols) + startX = cols; + if(startY<0) + startY=0; + if(startY > rows) + startY = rows; +// printf("rendering from %d/%d/%d to %d/%d --> %d/%d frame %d\n", +// rows,cols,frames,startX,startY,endX,endY, frame); + + int minVal = 16384; + int maxVal = -16384; + // get the max and min values first... + for (int y = 0; y < Height; y++) { + int ooby=0; + int ay = GetAY(y,Height,ooby); + for(int x=0;x= 4))){ + minVal = val; + } + if((val > maxVal) && (val <= 16380)){ + maxVal = val; + } + } + } + } + + maxVal++; + if(maxVal <= minVal) + maxVal = minVal+10; +// if(minVal > 0) +// minVal-=1; + maxPixVal=maxVal; + minPixVal=minVal; + int range = maxVal-minVal; +// if(range < 1) +// range=1; // don't divide by zero! +// printf("range = %d - %d --> %d\n",maxVal,minVal,range); + + for (int y = 0; y < Height; y++) { + uint *scanLine = + reinterpret_cast(image->scanLine(y)); + int ooby=0; + int ay = GetAY(y,Height,ooby); + for(int x=0;x (Width-scaleBarWidth+2)){ + int val = (Height-y-1) * ColormapSize / Height; + scanLine[x] = colormap[val % ColormapSize ]; + }else{ + scanLine[x] = 0;//colormap[0]; + } + }else{ + + int val = Get_Data(flow_based?flow:frame,ay,ax); +// if(ignore_pinned && ((val >= 16380) || (val <= 4))) +// scanLine[x]=colormap[0]; +// else{ + val -= minVal; + val *= ColormapSize; + val /= range; + uint cmap = colormap[val % ColormapSize]; + scanLine[x] = cmap; +// } + } + } + } + + // put the X's on the selected well's + if(rows && cols){ + for(int ts=0;ts= height()) + continue; + uint *scanLine = + reinterpret_cast(image->scanLine(marky)); + if(marky != y){ + scanLine[x]=xcolor; + }else{ + for(int markx=(x-pix);markx<(x+pix);markx++){ + if(markx < 0 || markx >= width()) + continue; + scanLine[markx]=xcolor; + } + } + } + + } + } + } + + UpdateTraceData(); + + return image; +} + +float SpatialPlot::Get_Data(int idx, int y, int x) +{ + (void)idx; + (void)y; + (void)x; + return 0; +} + +//! [10] +uint SpatialPlot::rgbFromWaveLength(double wave) +{ + double r = 0.0; + double g = 0.0; + double b = 0.0; + + if (wave >= 380.0 && wave <= 440.0) { + r = -1.0 * (wave - 440.0) / (440.0 - 380.0); + b = 1.0; + } else if (wave >= 440.0 && wave <= 490.0) { + g = (wave - 440.0) / (490.0 - 440.0); + b = 1.0; + } else if (wave >= 490.0 && wave <= 510.0) { + g = 1.0; + b = -1.0 * (wave - 510.0) / (510.0 - 490.0); + } else if (wave >= 510.0 && wave <= 580.0) { + r = (wave - 510.0) / (580.0 - 510.0); + g = 1.0; + } else if (wave >= 580.0 && wave <= 645.0) { + r = 1.0; + g = -1.0 * (wave - 645.0) / (645.0 - 580.0); + } else if (wave >= 645.0 && wave <= 780.0) { + r = 1.0; + } + + double s = 1.0; + if (wave > 700.0) + s = 0.3 + 0.7 * (780.0 - wave) / (780.0 - 700.0); + else if (wave < 420.0) + s = 0.3 + 0.7 * (wave - 380.0) / (420.0 - 380.0); + + r = pow(r * s, 0.8); + g = pow(g * s, 0.8); + b = pow(b * s, 0.8); + return qRgb(int(r * 255), int(g * 255), int(b * 255)); +} diff --git a/Analysis/DataViewer/SpatialPlot.h b/Analysis/DataViewer/SpatialPlot.h new file mode 100644 index 00000000..814c7457 --- /dev/null +++ b/Analysis/DataViewer/SpatialPlot.h @@ -0,0 +1,241 @@ +/* Copyright (C) 2016 Ion Torrent Systems, Inc. All Rights Reserved */ +/**************************************************************************** +** +** Copyright (C) 2016 The Qt Company Ltd. +** Contact: https://www.qt.io/licensing/ +** +** This file is part of the examples of the Qt Toolkit. +** +** $QT_BEGIN_LICENSE:BSD$ +** Commercial License Usage +** Licensees holding valid commercial Qt licenses may use this file in +** accordance with the commercial license agreement provided with the +** Software or, alternatively, in accordance with the terms contained in +** a written agreement between you and The Qt Company. For licensing terms +** and conditions see https://www.qt.io/terms-conditions. For further +** information use the contact form at https://www.qt.io/contact-us. +** +** BSD License Usage +** Alternatively, you may use this file under the terms of the BSD license +** as follows: +** +** "Redistribution and use in source and binary forms, with or without +** modification, are permitted provided that the following conditions are +** met: +** * Redistributions of source code must retain the above copyright +** notice, this list of conditions and the following disclaimer. +** * Redistributions in binary form must reproduce the above copyright +** notice, this list of conditions and the following disclaimer in +** the documentation and/or other materials provided with the +** distribution. +** * Neither the name of The Qt Company Ltd nor the names of its +** contributors may be used to endorse or promote products derived +** from this software without specific prior written permission. +** +** +** THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS +** "AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT +** LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR +** A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE COPYRIGHT +** OWNER OR CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, +** SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT +** LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF USE, +** DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY +** THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT +** (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE +** OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE." +** +** $QT_END_LICENSE$ +** +****************************************************************************/ + +#ifndef SPATIALPLOT_H +#define SPATIALPLOT_H + +#include +#include +#include +#include +#include +#include +#include "qcustomplot.h" + + +class SpatialPlot; + +#define MAX_NUM_TRACES 12 +#define MAX_TRACE_LEN 500 + +typedef struct{ + int y; + int x; +}TraceDescr_t; + + +//! [0] +class SpatialPlotThread : public QThread +{ + Q_OBJECT + +public: + SpatialPlotThread(QObject *parent = 0); + ~SpatialPlotThread(); + void Abort(); + void render(); + void SetParent(SpatialPlot *_parent); + + +signals: + void renderedImage(const QImage &image); + +protected: + void run() Q_DECL_OVERRIDE; + +private: + + QMutex mutex; + QWaitCondition condition; + bool restart=0; + bool abort=0; + SpatialPlot *mParent; + + +}; + +//! [0] +class SpatialPlot : public QWidget +{ + Q_OBJECT + +public: + SpatialPlot(QWidget *parent = 0); + ~SpatialPlot(); + void doConvertInt(); + virtual void doConvert(int &loading); + virtual void copyData(); + QImage *doRender(); + void copyLastCoord(); + void setRawTrace(int selection); + void setfilename(QString fileName); + void setSlider(int per); + void setTracePlot(QCustomPlot *TracePlot, int _flow_based=0); + + void keyPressEvent(QKeyEvent *event) Q_DECL_OVERRIDE; + void Save(QTextStream &strm); + virtual void SetOption(QString option, int state); + + static void SetBlockCoord(int x, int y){Block_Y=y;Block_X=x;} + +protected: + + virtual void DoubleClick(int x,int y); + virtual void DoubleClick_traces(int x, int y, int ts); + + void paintEvent(QPaintEvent *event) Q_DECL_OVERRIDE; + void resizeEvent(QResizeEvent *event) Q_DECL_OVERRIDE; +#ifndef QT_NO_WHEELEVENT + void wheelEvent(QWheelEvent *event) Q_DECL_OVERRIDE; +#endif +// void mousePressEvent(QMouseEvent *event) Q_DECL_OVERRIDE; + void mouseMoveEvent(QMouseEvent *event) Q_DECL_OVERRIDE; +// void mouseReleaseEvent(QMouseEvent *event) Q_DECL_OVERRIDE; + void render(); + uint rgbFromWaveLength(double wave); + enum { ColormapSize = 512 }; + uint colormap[ColormapSize]; + virtual float Get_Data(int idx, int y, int x); + + static TraceDescr_t traces[MAX_NUM_TRACES]; + static int traces_curSelection; + static int GblTracesVer; + + int LocalTracesVer=0; + float traces_Val[MAX_NUM_TRACES][MAX_TRACE_LEN]; + + float traces_ts[MAX_TRACE_LEN]={0}; + int _mTracePlotSet[MAX_TRACE_LEN]={0}; + int traces_len=0; + void UpdateTraceData(); + virtual void UpdateTracePlot(); + QCustomPlot *_mTracePlot=NULL; + int flow_based=0; + int ignore_pinned=0; + + +protected slots: + virtual void updatePixmap(const QImage &image); + void zoom(double zoomFactor, double XWeight=0.5, double YWeight=0.5); + void showPointToolTip(QMouseEvent *event); + void showPointToolTip_traces(QMouseEvent *event); + +private: + void mouseDoubleClickEvent(QMouseEvent *event); + void mouseDoubleClickEvent_traces(QMouseEvent *event); + + void mousePressEvent(QMouseEvent *); + void mouseReleaseEvent(QMouseEvent *); + void scroll(int deltaX, int deltaY); + int GetAY(int y, int Height, int & ooby); + int GetAX(int x, int Width, int & oobx); + + int GetY(int ay, int Height, int & ooby); + int GetX(int ax, int Width, int & oobx); + + SpatialPlotThread thread; + QPixmap pixmap; + QPoint pixmapOffset; + QPoint lastDragPos; + bool firstShow=false; + + // mouse location variables + int LastPressX=0; + int LastPressY=0; + float _mTraceRange=0; + + +protected: + // window variables + static int startX; + static int endX; + static int startY; + static int endY; + static int frame; + static int flow; + + static int Block_Y; + static int Block_X; + + int lastStartX=0; + int lastStartY=0; + int lastEndX=0; + int lastEndY=0; + int initialHints=1; + int loading=0; + + int maxPixVal=0; + int minPixVal=0; + + QString fname=""; + + // thread copy of app specific stuff + QString _fname=""; + QString last_fname=""; + + // image variables + static int rows; + static int cols; + static int frames; + static int flows; + + static const int borderHeight=20; + static const int borderWidth=30; + static const int scaleBarWidth=35; + + static uint16_t *refMask; //from BfMaskTab + static float *gainVals; //from GainTab + static char *mMask; // only display pixels with a 0 + +}; +//! [0] + +#endif // SPATIALPLOT_H diff --git a/Analysis/DataViewer/WellsSpatial.cpp b/Analysis/DataViewer/WellsSpatial.cpp new file mode 100644 index 00000000..2ae6b395 --- /dev/null +++ b/Analysis/DataViewer/WellsSpatial.cpp @@ -0,0 +1,97 @@ +/* Copyright (C) 2016 Ion Torrent Systems, Inc. All Rights Reserved */ +#include "WellsSpatial.h" +#include "RawWells.h" +#include +#include + +using namespace std; + +WellsSpatial::WellsSpatial(QWidget *parent): SpatialPlot(parent) +{ +} + +void WellsSpatial::SetOption(QString txt, int state) +{ + qDebug() << __PRETTY_FUNCTION__ << ": " << txt << state; + + if(txt == "Show Masked") + MaskReads = state; + + render(); +} + +void WellsSpatial::doConvert(int &loading) +{ + + if(fname == ""){ + free(out); + out=NULL; + last_fname = _fname; + traces_len=0; + } + if (last_fname != _fname){ + loading=1; + // load a new dat file + printf("triggered %s %s\n",last_fname.toLatin1().data(),_fname.toLatin1().data()); + fflush(stdout); + last_fname = _fname; + if(out) + free(out); + out=NULL; + + QByteArray ba = fname.toLatin1(); + char * fn = ba.data(); + + RawWells wells(fn,0,0); + //wells.SetSubsetToLoad(&col[0], &row[0], col.size()); + wells.OpenForRead(); + flows = wells.NumFlows(); + loading=2; +// LIMIT Flows + if(flows > 200) + flows = 200; +// LIMIT Flows + rows = wells.NumRows(); + cols = wells.NumCols(); + out = (float *)malloc(sizeof(float)*rows*cols*flows); + + cout << "#cols=" << cols << endl; + cout << "#rows=" << rows << endl; + cout << "#nFlow=" << flows << endl; + string flowOrder = wells.FlowOrder(); + cout << "#flowOrder=" << flowOrder << endl; + for(int row=0; rowflowValues[flow]; + } + } + } + + traces_len=flows; + for(int i=0;i +#include "SpatialPlot.h" +#include "qcustomplot.h" + +class WellsSpatial : public SpatialPlot +{ +public: + WellsSpatial(QWidget *parent); + void doConvert(int &loading); + void SetOption(QString option, int state); + +protected: + float Get_Data(int flow, int y, int x); + void DoubleClick_traces(int x, int y, int ts); + +private: + int MaskReads=0; + float *out=NULL; +}; + +#endif // WELLSSPATIAL_H diff --git a/Analysis/DataViewer/WellsTab.cpp b/Analysis/DataViewer/WellsTab.cpp new file mode 100644 index 00000000..705812b5 --- /dev/null +++ b/Analysis/DataViewer/WellsTab.cpp @@ -0,0 +1,30 @@ +/* Copyright (C) 2016 Ion Torrent Systems, Inc. All Rights Reserved */ +#include "WellsTab.h" + +static const maskCheckBox_t mcbt1[] = { + {"Show Masked",8,Qt::Unchecked} + +}; + +WellsTab::WellsTab(QString _mName, Dialog *_mParent, QWidget *parent) + : ModelTab(_mName, _mParent, parent) +{ + mSpatialPlot = new WellsSpatial(this); + + QGridLayout *grid = new QGridLayout; + grid->addWidget(MakeSlider("flows"),0,0); + grid->addWidget(MakeTraceCB(),1,0); + grid->addWidget(MakeList(mcbt1,sizeof(mcbt1)/sizeof(mcbt1[0])),0,1,2,1); + grid->setRowStretch(0,0); + grid->setRowStretch(1,0); + grid->addWidget(mSpatialPlot,2,0,1,4); + grid->addWidget(MakeTracePlot(1),3,0,1,4); + + // Set the graph styles to no line + for(int i=0;igraph(i)->setLineStyle(QCPGraph::lsNone); + mtracePlot->graph(i)->setScatterStyle(QCPScatterStyle(QCPScatterStyle::ssCircle,4)); + } + + setLayout(grid); +} diff --git a/Analysis/DataViewer/WellsTab.h b/Analysis/DataViewer/WellsTab.h new file mode 100644 index 00000000..d2970d18 --- /dev/null +++ b/Analysis/DataViewer/WellsTab.h @@ -0,0 +1,28 @@ +/* Copyright (C) 2016 Ion Torrent Systems, Inc. All Rights Reserved */ +#ifndef WELLSTAB_H +#define WELLSTAB_H + +#include +#include +#include "dialog.h" +#include "modeltab.h" +#include "WellsSpatial.h" +#include "AlignmentSpatial.h" + +class Dialog; + +class WellsTab : public ModelTab +{ + Q_OBJECT +public: + explicit WellsTab(QString _mName, Dialog *_mParent, QWidget *parent = 0); + +signals: + +public slots: + +private: + +}; + +#endif // WELLSTAB_H diff --git a/Analysis/DataViewer/dialog.cpp b/Analysis/DataViewer/dialog.cpp new file mode 100644 index 00000000..85c3421f --- /dev/null +++ b/Analysis/DataViewer/dialog.cpp @@ -0,0 +1,505 @@ +/* Copyright (C) 2016 Ion Torrent Systems, Inc. All Rights Reserved */ + +#include +#include + +#include "dialog.h" + +//! [0] +Dialog::Dialog() +{ + QTabWidget *tabWidget = new QTabWidget; + tabWidget->addTab((mRawTab = new RawTab("Dat",this)), tr("Raw")); + tabWidget->addTab((mBfMaskTab = new BfMaskTab("BfMask",this)), tr("BfMask")); + tabWidget->addTab((mNoiseTab = new NoiseTab("Noise",this)), tr("Noise")); + tabWidget->addTab((mGainTab = new GainTab("Gain",this)), tr("Gain")); + tabWidget->addTab((mWellsTab = new WellsTab("Wells",this)), tr("1.wells")); + tabWidget->addTab((mAlignmentTab = new AlignmentTab("Alignment",this)), tr("Alignment")); + + connect(tabWidget,SIGNAL(currentChanged(int)), this, SLOT(currentChanged(int))); + + QVBoxLayout *mainLayout = new QVBoxLayout; + mainLayout->addWidget(tabWidget); + + setLayout(mainLayout); + setCentralWidget(tabWidget); + + + setWindowTitle(tr("Ion Dat Explorer")); + QMenu *fileMenu = menuBar()->addMenu(tr("&File")); + + QAction *dummyAct = new QAction(tr(""),this); + fileMenu->addAction(dummyAct); + + OpenDatAct = new QAction(tr("&Open Dat file"), this); + OpenDatAct->setStatusTip(tr("Open Dat file")); + connect(OpenDatAct, SIGNAL(triggered()), this, SLOT(browseDatDir())); + fileMenu->addAction(OpenDatAct); + + OpenBfMaskAct = new QAction(tr("&Open BfMask file"), this); + OpenBfMaskAct->setStatusTip(tr("Open Dat file")); + connect(OpenBfMaskAct, SIGNAL(triggered()), this, SLOT(browseBfMaskDir())); + fileMenu->addAction(OpenBfMaskAct); + + OpenNoiseAct = new QAction(tr("&Open Noise file"), this); + OpenNoiseAct->setStatusTip(tr("Open Noise file")); + connect(OpenNoiseAct, SIGNAL(triggered()), this, SLOT(browseNoiseDir())); + fileMenu->addAction(OpenNoiseAct); + + OpenGainAct = new QAction(tr("&Open Gain file"), this); + OpenGainAct->setStatusTip(tr("Open Gain file")); + connect(OpenGainAct, SIGNAL(triggered()), this, SLOT(browseGainDir())); + fileMenu->addAction(OpenGainAct); + + OpenWellsAct = new QAction(tr("&Open Wells file"), this); + OpenWellsAct->setStatusTip(tr("Open Wells file")); + connect(OpenWellsAct, SIGNAL(triggered()), this, SLOT(browseWellsDir())); + fileMenu->addAction(OpenWellsAct); + + OpenBamAct = new QAction(tr("&Open Bam file"), this); + OpenBamAct->setStatusTip(tr("Open Bam file")); + connect(OpenBamAct, SIGNAL(triggered()), this, SLOT(browseBamDir())); + fileMenu->addAction(OpenBamAct); + + QAction *ClearAct = new QAction(tr("&Clear"), this); + ClearAct->setStatusTip(tr("Clear")); + connect(ClearAct, SIGNAL(triggered()), this, SLOT(Clear())); + fileMenu->addAction(ClearAct); + + dummyAct = new QAction(tr(""),this); + fileMenu->addAction(dummyAct); + QAction *SaveAct = new QAction(tr("&SaveData"), this); + SaveAct->setStatusTip(tr("SaveData")); + connect(SaveAct, SIGNAL(triggered()), this, SLOT(Save())); + fileMenu->addAction(SaveAct); + dummyAct = new QAction(tr(""),this); + fileMenu->addAction(dummyAct); + + QAction *aboutAct = new QAction(tr("&about"), this); + aboutAct->setStatusTip(tr("Create a new file")); + connect(aboutAct, SIGNAL(triggered()), this, SLOT(about())); + fileMenu->addAction(aboutAct); + + resize(800, 800); + +} +//! [0] + +void Dialog::Clear() +{ + DatFileName=""; + BfMaskFileName=""; + NoiseFileName=""; + GainFileName=""; + WellsFileName=""; + RawExpmtDir = ""; + RawExpmt_X = -1; + RawExpmt_Y = -1; + SpatialPlot::SetBlockCoord(RawExpmt_X,RawExpmt_Y); + AutoCompletePaths(); + +} + + +void Dialog::Save() +{ + QString fileName = QFileDialog::getSaveFileName(this, + tr("Save Charts File"), "","*.csv"); + + printf("%s: %s\n",__FUNCTION__,fileName.toUtf8().constData()); + if (!fileName.isEmpty()) { + // save all the charts to this file.. + QFile file(fileName); + if (file.open(QIODevice::WriteOnly)) { +// std::cerr << "Cannot open file for writing: " +// << qPrintable(file.errorString()) << std::endl; + QTextStream out(&file); + + mRawTab->Save(out); + mBfMaskTab->Save(out); + mWellsTab->Save(out); + mNoiseTab->Save(out); + mGainTab->Save(out); + mAlignmentTab->Save(out); + file.close(); + } + } +} + +void Dialog::about() +//! [13] //! [14] +{ + QMessageBox::about(this, tr("About Application"), + tr("Ion Data Viewer Application \n" + "Version 0.1")); +} + +void Dialog::browseDatDir() +{ + QString fileName = QFileDialog::getOpenFileName(this, + tr("Open Dat File"), DatFileName,"*.dat*"); + + printf("%s: %s\n",__FUNCTION__,fileName.toLatin1().data()); + if (!fileName.isEmpty()) { + DatFileName=fileName; + mRawTab->setfilename(DatFileName); + + char tmpStr[2048]; + + strcpy(tmpStr,DatFileName.toLatin1().data()); + + char *tmp=tmpStr; + char *prev1=NULL; + char *prev2=NULL; + + while((tmp = strstr(tmp,"/"))){ + prev2=prev1; + prev1=tmp; + tmp++; + } + if(prev2 && prev2[1] == 'X'){ + int X=0; + int Y=0; + prev2[0]=0; + prev2++; + // prev2 points to the block + sscanf(prev2,"X%d_Y%d",&X,&Y); + RawExpmtDir = tmpStr; + RawExpmt_X = X; + RawExpmt_Y = Y; + SpatialPlot::SetBlockCoord(RawExpmt_X,RawExpmt_Y); + printf("%s: X=%d Y=%d basedir=%s\n",__FUNCTION__,X,Y,tmpStr); + fflush(stdout); + }else if(prev2 && prev2[1] == 't'){ + // thumbnail + thumbnail=1; + int X=0; + int Y=0; + prev2[0]=0; + RawExpmtDir = tmpStr; + RawExpmt_X = X; + RawExpmt_Y = Y; + SpatialPlot::SetBlockCoord(RawExpmt_X,RawExpmt_Y); + printf("%s: X=%d Y=%d basedir=%s\n",__FUNCTION__,X,Y,tmpStr); + fflush(stdout); + + } + AutoCompletePaths(); + currentChanged(0); + } +} + +void Dialog::browseBfMaskDir() +{ + QString fileName = QFileDialog::getOpenFileName(this, + tr("Open BfMask File"), BfMaskFileName,"*.bin*"); + + printf("%s: %s\n",__FUNCTION__,fileName.toLatin1().data()); + if (!fileName.isEmpty()) { + BfMaskFileName=fileName; + mBfMaskTab->setfilename(BfMaskFileName); + currentChanged(1); + } +} + +void Dialog::browseNoiseDir() +{ + QString fileName = QFileDialog::getOpenFileName(this, + tr("Open Noise File"), NoiseFileName,"NoisePic3.dat"); + + printf("%s: %s\n",__FUNCTION__,fileName.toLatin1().data()); + if (!fileName.isEmpty()) { + NoiseFileName=fileName; + mNoiseTab->setfilename(NoiseFileName); + currentChanged(2); + } +} + +void Dialog::browseGainDir() +{ + QString fileName = QFileDialog::getOpenFileName(this, + tr("Open Gain File"), GainFileName,"Gain.lsr"); + + printf("%s: %s\n",__FUNCTION__,fileName.toLatin1().data()); + if (!fileName.isEmpty()) { + GainFileName=fileName; + mGainTab->setfilename(GainFileName); + currentChanged(3); + } +} + + +void Dialog::browseWellsDir() +{ + QString fileName = QFileDialog::getOpenFileName(this, + tr("Open Wells File"), WellsFileName,"*.wells"); + + printf("%s: %s\n",__FUNCTION__,fileName.toLatin1().data()); + if (!fileName.isEmpty()) { + WellsFileName=fileName; + mWellsTab->setfilename(WellsFileName); + currentChanged(4); + + char tmpStr[2048]; + char *ptr=NULL; + int found=0; + + strcpy(tmpStr,WellsFileName.toLatin1().data()); + + if((ptr = strstr(tmpStr,"/onboard_results/sigproc_results"))){ + *ptr=0; + found=1; + // were on the instrument?? + } + else if((ptr = strstr(tmpStr,"/sigproc_results"))){ + *ptr=0; + found=1; + thumbnail=1; + OutputDir = tmpStr; + RawExpmtDir = tmpStr + QString("/rawdata/"); + RawExpmt_X = 0; + RawExpmt_Y = 0; + SpatialPlot::SetBlockCoord(RawExpmt_X,RawExpmt_Y); + printf("%s: RawExpmtDir = %s\n",__FUNCTION__,RawExpmtDir.toLatin1().data()); + } + + if(found) + AutoCompletePaths(); + +// char *tmp=tmpStr; +// char *prev1=NULL; +// char *prev2=NULL; +// +// while((tmp = strstr(tmp,"/"))){ +// prev2=prev1; +// prev1=tmp; +// tmp++; +// } +// if(prev2 && prev2[1] == 'X'){ +// int X=0; +// int Y=0; +// prev2[0]=0; +// prev2++; +// // prev2 points to the block +// sscanf(prev2,"X%d_Y%d",&X,&Y); +// RawExpmtDir = tmpStr; +// RawExpmt_X = X; +// RawExpmt_Y = Y; +// printf("%s: X=%d Y=%d basedir=%s\n",__FUNCTION__,X,Y,tmpStr); +// AutoCompletePaths(); +// fflush(stdout); +// } + } +} + +void Dialog::browseBamDir() +{ + QString fileName = QFileDialog::getOpenFileName(this, + tr("Open Bam File"), BamFileName,"*.bam"); + + printf("%s: %s\n",__FUNCTION__,fileName.toLatin1().data()); + if (!fileName.isEmpty()) { + QFileInfo file(fileName); + //file.absoluteDir(); + BamFileName=file.absolutePath(); + mAlignmentTab->setfilename(BamFileName); + currentChanged(5); + } +} + +void Dialog::currentChanged(int index) +{ + QString MainString = "Ion Dat Explorer "; + if(index == 0) + MainString += "(" + DatFileName + ")"; + else if(index == 1) + MainString += "(" + BfMaskFileName + ")"; + else if(index == 2) + MainString += "(" + WellsFileName + ")"; + else if(index == 3) + MainString += "(" + BamFileName + ")"; + + setWindowTitle(MainString); + +} + +void Dialog::AutoCompletePaths() +{ + qDebug() << __PRETTY_FUNCTION__ << ": RawExpmtDir=" << RawExpmtDir; + qDebug() << __PRETTY_FUNCTION__ << ": OutputDir=" << OutputDir; + + if(RawExpmtDir != "" && OutputDir == ""){ + // try to guess the output dir.. + QString opdir=RawExpmtDir + "/sigproc_results/"; + QFileInfo chk1(opdir); + if(chk1.exists() && chk1.isDir()){ + OutputDir=opdir; + qDebug() << __PRETTY_FUNCTION__ << ": OutputDir=" << OutputDir; + } + } + if(RawExpmtDir != "" && OutputDir == "" && !thumbnail){ + // try to guess the output dir.. + QString opdir=RawExpmtDir + "/onboard_results/sigproc_results/"; + QFileInfo chk1(opdir); + if(chk1.exists() && chk1.isDir()){ + OutputDir=opdir; + qDebug() << __PRETTY_FUNCTION__ << ": OutputDir=" << OutputDir; + } + } + if(RawExpmtDir != "" && OutputDir == ""){ + // try to guess the output dir.. + QString tmp = RawExpmtDir; + int pos = tmp.indexOf("/rawdata"); + if(pos){ + tmp.replace("/rawdata",""); + if(tmp != RawExpmtDir){ + QString opdir=tmp + "/sigproc_results/"; + QFileInfo chk1(opdir); + if(chk1.exists() && chk1.isDir()){ + OutputDir=tmp; + qDebug() << __PRETTY_FUNCTION__ << ": OutputDir=" << OutputDir; + } + } + } + } + + QString BlockName = "X" + QString::number(RawExpmt_X) + + "_Y" + QString::number(RawExpmt_Y); + if(thumbnail) + BlockName="thumbnail"; + + qDebug() << __PRETTY_FUNCTION__ << ": BlockName=" << BlockName; + + if(DatFileName == "" && + RawExpmtDir != "" && + RawExpmt_X != -1 && + RawExpmt_Y != -1){ + // auto-fill in the file + QString tmp = RawExpmtDir + "/" + BlockName + "/acq_0000.dat"; + QFileInfo check_file(tmp); + if (check_file.exists() && check_file.isFile()) { + DatFileName = tmp; + } + } + + if(NoiseFileName == "" && + RawExpmtDir != ""){ + // auto-fill in the file + QString tmp = RawExpmtDir + "/NoisePic3.dat"; + QFileInfo check_file(tmp); + if (check_file.exists() && check_file.isFile()) { + NoiseFileName = tmp; + } + } + + if(GainFileName == "" && + RawExpmtDir != "" && + RawExpmt_X != -1 && + RawExpmt_Y != -1){ + // auto-fill in the file + QString tmp = RawExpmtDir + "/" + BlockName + "/Gain.lsr"; + QFileInfo check_file(tmp); + if (check_file.exists() && check_file.isFile()) { + GainFileName = tmp; + } + } + + if(BfMaskFileName == "" && + RawExpmtDir != "" && + RawExpmt_X != -1 && + RawExpmt_Y != -1){ + // auto-fill in the file + QString tmp = OutputDir + "/block_" + BlockName + "/bfmask.bin"; + QFileInfo check_file(tmp); + if (check_file.exists() && check_file.isFile()) { + BfMaskFileName = tmp; + } + else{ + tmp = OutputDir + "/sigproc_results/bfmask.bin"; + QFileInfo check_file2(tmp); + if (check_file2.exists() && check_file2.isFile()) { + BfMaskFileName = tmp; + } + } + } + + if(WellsFileName == "" && + RawExpmtDir != "" && + RawExpmt_X != -1 && + RawExpmt_Y != -1){ + // auto-fill in the file + QString tmp = OutputDir + "/block_" + BlockName + "/1.wells"; + if(thumbnail){ + tmp = OutputDir + "/sigproc_results/1.wells"; + } + QFileInfo check_file(tmp); + if (check_file.exists() && check_file.isFile()) { + WellsFileName = tmp; + } + } + + if(BamFileName == "" && OutputDir != ""){ + QString tmpName=OutputDir; + if(RawExpmtDir != ""){ + // see if rawdata is in the file path.. + const char *tmp = RawExpmtDir.toUtf8().constData(); + char tmpCname[4096]; + strcpy(tmpCname,tmp); + char *ptr = strstr(tmpCname,"/rawdata"); + if(ptr){ + *ptr=0; + tmpName=tmpCname; + } + } + + BamFileName = tmpName; + } + + + + QString tmp=""; + + tmp = "&Open Dat file"; + if(DatFileName != ""){ + tmp += " (" + DatFileName + ")"; + } + OpenDatAct->setText(tmp); + mRawTab->setfilename(DatFileName); + + tmp = "&Open BfMask file"; + if(BfMaskFileName != ""){ + tmp += " (" + BfMaskFileName + ")"; + } + OpenBfMaskAct->setText(tmp); + mBfMaskTab->setfilename(BfMaskFileName); + + tmp = "&Open Noise file"; + if(NoiseFileName != ""){ + tmp += " (" + NoiseFileName + ")"; + } + OpenNoiseAct->setText(tmp); + mNoiseTab->setfilename(NoiseFileName); + + tmp = "&Open Gain file"; + if(GainFileName != ""){ + tmp += " (" + GainFileName + ")"; + } + OpenGainAct->setText(tmp); + mGainTab->setfilename(GainFileName); + + tmp = "&Open Wells file"; + if(WellsFileName != ""){ + tmp += " (" + WellsFileName + ")"; + } + OpenWellsAct->setText(tmp); + mWellsTab->setfilename(WellsFileName); + + tmp = "&Open Bam file"; + if(BamFileName != ""){ + tmp += " (" + BamFileName + ")"; + } + OpenBamAct->setText(tmp); + mAlignmentTab->setfilename(BamFileName); +} + + diff --git a/Analysis/DataViewer/dialog.h b/Analysis/DataViewer/dialog.h new file mode 100644 index 00000000..feccf06d --- /dev/null +++ b/Analysis/DataViewer/dialog.h @@ -0,0 +1,97 @@ +/* Copyright (C) 2016 Ion Torrent Systems, Inc. All Rights Reserved */ + +#ifndef DIALOG_H +#define DIALOG_H + +#include +#include +#include "RawTab.h" +#include "AlignmentTab.h" +#include "BfMaskTab.h" +#include "WellsTab.h" +#include "NoiseTab.h" +#include "GainTab.h" + + +QT_BEGIN_NAMESPACE +class QAction; +class QDialogButtonBox; +class QGroupBox; +class QLabel; +class QLineEdit; +class QMenu; +class QMenuBar; +class QPushButton; +class QTextEdit; +class QComboBox; +QT_END_NAMESPACE + +class FileTab; +class RawTab; +class AlignmentTab; +class BfMaskTab; +class NoiseTab; +class GainTab; +class WellsTab; + +//! [0] +class Dialog : public QMainWindow //QDialog +{ + Q_OBJECT + +public: + Dialog(); + int GetRawExpmt_Y(){return RawExpmt_Y;} + int GetRawExpmt_X(){return RawExpmt_X;} + +private slots: + void about(); + void browseDatDir(); + void browseBfMaskDir(); + void browseNoiseDir(); + void browseGainDir(); + void browseWellsDir(); + void browseBamDir(); + void currentChanged(int index); + void Clear(); + void Save(); + +private: + void AutoCompletePaths(); + + QString RawExpmtDir=""; + QString OutputDir=""; + int RawExpmt_X=-1; + int RawExpmt_Y=-1; + int thumbnail = 0; +// int RawExpmt_W=-1; +// int RawExpmt_H=-1; + + FileTab *mFileTab; + RawTab *mRawTab; + BfMaskTab *mBfMaskTab; + WellsTab *mWellsTab; + NoiseTab *mNoiseTab; + GainTab *mGainTab; + AlignmentTab *mAlignmentTab; + QString DatFileName=""; + QString BfMaskFileName=""; + QString NoiseFileName=""; + QString GainFileName=""; + QString WellsFileName=""; + QString BamFileName=""; + + QAction *OpenDatAct=NULL; + QAction *OpenBfMaskAct=NULL; + QAction *OpenNoiseAct=NULL; + QAction *OpenGainAct=NULL; + QAction *OpenWellsAct=NULL; + QAction *OpenBamAct=NULL; + + +}; +//! [0] + + + +#endif // DIALOG_H diff --git a/Analysis/DataViewer/main.cpp b/Analysis/DataViewer/main.cpp new file mode 100644 index 00000000..181b2780 --- /dev/null +++ b/Analysis/DataViewer/main.cpp @@ -0,0 +1,22 @@ +/* Copyright (C) 2016 Ion Torrent Systems, Inc. All Rights Reserved */ + +#include "dialog.h" + +#include + +#include +#include + + +//! [0] +int main(int argc, char *argv[]) +{ + QApplication app(argc, argv); + + Dialog dialog; + + dialog.show(); + + return app.exec(); +} +//! [0] diff --git a/Analysis/DataViewer/main.qml b/Analysis/DataViewer/main.qml new file mode 100644 index 00000000..930c6e79 --- /dev/null +++ b/Analysis/DataViewer/main.qml @@ -0,0 +1,16 @@ +import QtQuick 2.7 +import QtQuick.Window 2.2 + +Window { + visible: true + width: 640 + height: 480 + title: qsTr("Hello World") + + MainForm { + anchors.fill: parent + mouseArea.onClicked: { + console.log(qsTr('Clicked on background. Text: "' + textEdit.text + '"')) + } + } +} diff --git a/Analysis/DataViewer/modeltab.cpp b/Analysis/DataViewer/modeltab.cpp new file mode 100644 index 00000000..63976ffa --- /dev/null +++ b/Analysis/DataViewer/modeltab.cpp @@ -0,0 +1,123 @@ +/* Copyright (C) 2016 Ion Torrent Systems, Inc. All Rights Reserved */ +#include "modeltab.h" + +ModelTab::ModelTab(QString _mName, Dialog *_mParent, QWidget *parent) : QWidget(parent) +{ + mParent = _mParent; + mName=_mName; +} + +QComboBox *ModelTab::MakeTraceCB() +{ + mTraceComboBox = new QComboBox; + mTraceComboBox->setEditable(false); + for(int i=0;iaddItem(tr("Trace ") + QString::number(i)); + } + mTraceComboBox->setFixedWidth(250); +// mTraceComboBox->setFixedHeight(24); + + connect(mTraceComboBox, SIGNAL(currentIndexChanged(QString)),this,SLOT(mTraceCombobox_currentIndexChanged(QString))); + return mTraceComboBox; +} + +QTableView *ModelTab::MakeList(const maskCheckBox_t *mcbt, int nitems) +{ + QStandardItemModel *model = new QStandardItemModel(5, 3); + int x=0; + int y=0; + for (int r = 0; r < nitems; ++r) + { + item[r] = new QStandardItem(mcbt[r].name); + + item[r]->setFlags(Qt::ItemIsUserCheckable | Qt::ItemIsEnabled); + item[r]->setData(mcbt[r].state, Qt::CheckStateRole); + if(mcbt[r].state == Qt::Checked) + mSpatialPlot->SetOption(mcbt[r].name,1); + model->setItem(y++, x, item[r]); + if(y > 4){ + x++; + y=0; + } + } + + connect(model, SIGNAL(dataChanged ( const QModelIndex&, const QModelIndex&)), this, SLOT(slot_changed(const QModelIndex&, const QModelIndex&))); + + QTableView *list = new QTableView(); + list->setShowGrid(false); + QHeaderView *verticalHeader = list->verticalHeader(); + verticalHeader->sectionResizeMode(QHeaderView::Fixed); + verticalHeader->setDefaultSectionSize(15); + verticalHeader->hide(); + list->horizontalHeader()->hide(); + + list->setModel(model); + list->horizontalHeader()->setSectionResizeMode(0,QHeaderView::Stretch); + list->horizontalHeader()->setSectionResizeMode(1,QHeaderView::Stretch); + list->horizontalHeader()->setSectionResizeMode(2,QHeaderView::Stretch); +// list->setFixedWidth(350); + list->setFixedHeight(78); + return (list); +} + + + +QCustomPlot *ModelTab::MakeTracePlot(int flow_based) +{ + mtracePlot = new QCustomPlot(this); + mSpatialPlot->setTracePlot(mtracePlot,flow_based); + return mtracePlot; +} + + +QGroupBox *ModelTab::MakeSlider(QString name) +{ + QGroupBox *frameBox = new QGroupBox(name); + QVBoxLayout *vframeBox = new QVBoxLayout; + + mSlider = new QSlider(Qt::Horizontal); + connect(mSlider, SIGNAL(valueChanged(int)), this, SLOT(SliderChanged(int))); + vframeBox->addWidget(mSlider); + mSlider->setFocusPolicy(Qt::NoFocus); + frameBox->setLayout(vframeBox); + frameBox->setMaximumHeight(50); + frameBox->setMaximumWidth(250); + if(name == ""){ + mSlider->hide(); + } + return(frameBox); +} + +void ModelTab::SliderChanged(int k) +{ + printf("%s: %d\n",__FUNCTION__,k); + mSpatialPlot->setSlider(k); +} + +void ModelTab::mTraceCombobox_currentIndexChanged(const QString &txt) +{ + printf("%s: %s\n",__FUNCTION__,txt.toLatin1().data()); + fflush(stdout); + int selection=0; + sscanf(txt.toLatin1().data(),"Trace %d",&selection); + mSpatialPlot->setRawTrace(selection); +} + +void ModelTab::setfilename(QString filename) +{ + printf("%s:\n",__FUNCTION__); + mSpatialPlot->setfilename(filename); +} + +void ModelTab::slot_changed(const QModelIndex& topLeft, const QModelIndex&bootmRight) +{ + (void)bootmRight; + int index=topLeft.column()*5+topLeft.row(); + qDebug() << __PRETTY_FUNCTION__ << ": Item " << index << " "; + +// if(index < mNitems) + { + bool state = (item[index]->checkState() == Qt::Checked); + mSpatialPlot->SetOption(item[index]->text(),state); + } +} diff --git a/Analysis/DataViewer/modeltab.h b/Analysis/DataViewer/modeltab.h new file mode 100644 index 00000000..b56a7733 --- /dev/null +++ b/Analysis/DataViewer/modeltab.h @@ -0,0 +1,52 @@ +/* Copyright (C) 2016 Ion Torrent Systems, Inc. All Rights Reserved */ +#ifndef MODELTAB_H +#define MODELTAB_H + +#include +#include +#include "qcustomplot.h" +#include "SpatialPlot.h" +//#include "dialog.h" + +typedef struct { + const char *name; + int option; + Qt::CheckState state; +}maskCheckBox_t; + +class Dialog; + +class ModelTab : public QWidget +{ + Q_OBJECT +public: + explicit ModelTab(QString _mName, Dialog *_mParent, QWidget *parent = 0); + void setfilename(QString filename); + void Save(QTextStream &strm){strm << mName << endl; mSpatialPlot->Save(strm);} + +signals: + +public slots: + void SliderChanged(int k); + void mTraceCombobox_currentIndexChanged(const QString &txt); + void slot_changed(const QModelIndex& topLeft, const QModelIndex&bootmRight); + +protected: + QTableView *MakeList(const maskCheckBox_t *mcbt, int nitems); + QComboBox *MakeTraceCB(); + QGroupBox *MakeSlider(QString name); + QCustomPlot *MakeTracePlot(int flow_based=0); + + QString mName=""; + QComboBox* combo; + + Dialog *mParent; + QSlider *mSlider; + SpatialPlot *mSpatialPlot; + QCustomPlot *mtracePlot; + QStandardItem* item[25]={NULL}; + QComboBox *mTraceComboBox; + +}; + +#endif // MODELTAB_H diff --git a/Analysis/DataViewer/qcustomplot.cpp b/Analysis/DataViewer/qcustomplot.cpp new file mode 100644 index 00000000..30860827 --- /dev/null +++ b/Analysis/DataViewer/qcustomplot.cpp @@ -0,0 +1,23553 @@ +/*************************************************************************** +** ** +** QCustomPlot, an easy to use, modern plotting widget for Qt ** +** Copyright (C) 2011-2015 Emanuel Eichhammer ** +** ** +** This program is free software: you can redistribute it and/or modify ** +** it under the terms of the GNU General Public License as published by ** +** the Free Software Foundation, either version 3 of the License, or ** +** (at your option) any later version. ** +** ** +** This program is distributed in the hope that it will be useful, ** +** but WITHOUT ANY WARRANTY; without even the implied warranty of ** +** MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the ** +** GNU General Public License for more details. ** +** ** +** You should have received a copy of the GNU General Public License ** +** along with this program. If not, see http://www.gnu.org/licenses/. ** +** ** +**************************************************************************** +** Author: Emanuel Eichhammer ** +** Website/Contact: http://www.qcustomplot.com/ ** +** Date: 22.12.15 ** +** Version: 1.3.2 ** +****************************************************************************/ + +#include "qcustomplot.h" + + + +//////////////////////////////////////////////////////////////////////////////////////////////////// +//////////////////// QCPPainter +//////////////////////////////////////////////////////////////////////////////////////////////////// + +/*! \class QCPPainter + \brief QPainter subclass used internally + + This QPainter subclass is used to provide some extended functionality e.g. for tweaking position + consistency between antialiased and non-antialiased painting. Further it provides workarounds + for QPainter quirks. + + \warning This class intentionally hides non-virtual functions of QPainter, e.g. setPen, save and + restore. So while it is possible to pass a QCPPainter instance to a function that expects a + QPainter pointer, some of the workarounds and tweaks will be unavailable to the function (because + it will call the base class implementations of the functions actually hidden by QCPPainter). +*/ + +/*! + Creates a new QCPPainter instance and sets default values +*/ +QCPPainter::QCPPainter() : + QPainter(), + mModes(pmDefault), + mIsAntialiasing(false) +{ + // don't setRenderHint(QPainter::NonCosmeticDefautPen) here, because painter isn't active yet and + // a call to begin() will follow +} + +/*! + Creates a new QCPPainter instance on the specified paint \a device and sets default values. Just + like the analogous QPainter constructor, begins painting on \a device immediately. + + Like \ref begin, this method sets QPainter::NonCosmeticDefaultPen in Qt versions before Qt5. +*/ +QCPPainter::QCPPainter(QPaintDevice *device) : + QPainter(device), + mModes(pmDefault), + mIsAntialiasing(false) +{ +#if QT_VERSION < QT_VERSION_CHECK(5, 0, 0) // before Qt5, default pens used to be cosmetic if NonCosmeticDefaultPen flag isn't set. So we set it to get consistency across Qt versions. + if (isActive()) + setRenderHint(QPainter::NonCosmeticDefaultPen); +#endif +} + +QCPPainter::~QCPPainter() +{ +} + +/*! + Sets the pen of the painter and applies certain fixes to it, depending on the mode of this + QCPPainter. + + \note this function hides the non-virtual base class implementation. +*/ +void QCPPainter::setPen(const QPen &pen) +{ + QPainter::setPen(pen); + if (mModes.testFlag(pmNonCosmetic)) + makeNonCosmetic(); +} + +/*! \overload + + Sets the pen (by color) of the painter and applies certain fixes to it, depending on the mode of + this QCPPainter. + + \note this function hides the non-virtual base class implementation. +*/ +void QCPPainter::setPen(const QColor &color) +{ + QPainter::setPen(color); + if (mModes.testFlag(pmNonCosmetic)) + makeNonCosmetic(); +} + +/*! \overload + + Sets the pen (by style) of the painter and applies certain fixes to it, depending on the mode of + this QCPPainter. + + \note this function hides the non-virtual base class implementation. +*/ +void QCPPainter::setPen(Qt::PenStyle penStyle) +{ + QPainter::setPen(penStyle); + if (mModes.testFlag(pmNonCosmetic)) + makeNonCosmetic(); +} + +/*! \overload + + Works around a Qt bug introduced with Qt 4.8 which makes drawing QLineF unpredictable when + antialiasing is disabled. Thus when antialiasing is disabled, it rounds the \a line to + integer coordinates and then passes it to the original drawLine. + + \note this function hides the non-virtual base class implementation. +*/ +void QCPPainter::drawLine(const QLineF &line) +{ + if (mIsAntialiasing || mModes.testFlag(pmVectorized)) + QPainter::drawLine(line); + else + QPainter::drawLine(line.toLine()); +} + +/*! + Sets whether painting uses antialiasing or not. Use this method instead of using setRenderHint + with QPainter::Antialiasing directly, as it allows QCPPainter to regain pixel exactness between + antialiased and non-antialiased painting (Since Qt < 5.0 uses slightly different coordinate systems for + AA/Non-AA painting). +*/ +void QCPPainter::setAntialiasing(bool enabled) +{ + setRenderHint(QPainter::Antialiasing, enabled); + if (mIsAntialiasing != enabled) + { + mIsAntialiasing = enabled; + if (!mModes.testFlag(pmVectorized)) // antialiasing half-pixel shift only needed for rasterized outputs + { + if (mIsAntialiasing) + translate(0.5, 0.5); + else + translate(-0.5, -0.5); + } + } +} + +/*! + Sets the mode of the painter. This controls whether the painter shall adjust its + fixes/workarounds optimized for certain output devices. +*/ +void QCPPainter::setModes(QCPPainter::PainterModes modes) +{ + mModes = modes; +} + +/*! + Sets the QPainter::NonCosmeticDefaultPen in Qt versions before Qt5 after beginning painting on \a + device. This is necessary to get cosmetic pen consistency across Qt versions, because since Qt5, + all pens are non-cosmetic by default, and in Qt4 this render hint must be set to get that + behaviour. + + The Constructor \ref QCPPainter(QPaintDevice *device) which directly starts painting also sets + the render hint as appropriate. + + \note this function hides the non-virtual base class implementation. +*/ +bool QCPPainter::begin(QPaintDevice *device) +{ + bool result = QPainter::begin(device); +#if QT_VERSION < QT_VERSION_CHECK(5, 0, 0) // before Qt5, default pens used to be cosmetic if NonCosmeticDefaultPen flag isn't set. So we set it to get consistency across Qt versions. + if (result) + setRenderHint(QPainter::NonCosmeticDefaultPen); +#endif + return result; +} + +/*! \overload + + Sets the mode of the painter. This controls whether the painter shall adjust its + fixes/workarounds optimized for certain output devices. +*/ +void QCPPainter::setMode(QCPPainter::PainterMode mode, bool enabled) +{ + if (!enabled && mModes.testFlag(mode)) + mModes &= ~mode; + else if (enabled && !mModes.testFlag(mode)) + mModes |= mode; +} + +/*! + Saves the painter (see QPainter::save). Since QCPPainter adds some new internal state to + QPainter, the save/restore functions are reimplemented to also save/restore those members. + + \note this function hides the non-virtual base class implementation. + + \see restore +*/ +void QCPPainter::save() +{ + mAntialiasingStack.push(mIsAntialiasing); + QPainter::save(); +} + +/*! + Restores the painter (see QPainter::restore). Since QCPPainter adds some new internal state to + QPainter, the save/restore functions are reimplemented to also save/restore those members. + + \note this function hides the non-virtual base class implementation. + + \see save +*/ +void QCPPainter::restore() +{ + if (!mAntialiasingStack.isEmpty()) + mIsAntialiasing = mAntialiasingStack.pop(); + else + qDebug() << Q_FUNC_INFO << "Unbalanced save/restore"; + QPainter::restore(); +} + +/*! + Changes the pen width to 1 if it currently is 0. This function is called in the \ref setPen + overrides when the \ref pmNonCosmetic mode is set. +*/ +void QCPPainter::makeNonCosmetic() +{ + if (qFuzzyIsNull(pen().widthF())) + { + QPen p = pen(); + p.setWidth(1); + QPainter::setPen(p); + } +} + + +//////////////////////////////////////////////////////////////////////////////////////////////////// +//////////////////// QCPScatterStyle +//////////////////////////////////////////////////////////////////////////////////////////////////// + +/*! \class QCPScatterStyle + \brief Represents the visual appearance of scatter points + + This class holds information about shape, color and size of scatter points. In plottables like + QCPGraph it is used to store how scatter points shall be drawn. For example, \ref + QCPGraph::setScatterStyle takes a QCPScatterStyle instance. + + A scatter style consists of a shape (\ref setShape), a line color (\ref setPen) and possibly a + fill (\ref setBrush), if the shape provides a fillable area. Further, the size of the shape can + be controlled with \ref setSize. + + \section QCPScatterStyle-defining Specifying a scatter style + + You can set all these configurations either by calling the respective functions on an instance: + \snippet documentation/doc-code-snippets/mainwindow.cpp qcpscatterstyle-creation-1 + + Or you can use one of the various constructors that take different parameter combinations, making + it easy to specify a scatter style in a single call, like so: + \snippet documentation/doc-code-snippets/mainwindow.cpp qcpscatterstyle-creation-2 + + \section QCPScatterStyle-undefinedpen Leaving the color/pen up to the plottable + + There are two constructors which leave the pen undefined: \ref QCPScatterStyle() and \ref + QCPScatterStyle(ScatterShape shape, double size). If those constructors are used, a call to \ref + isPenDefined will return false. It leads to scatter points that inherit the pen from the + plottable that uses the scatter style. Thus, if such a scatter style is passed to QCPGraph, the line + color of the graph (\ref QCPGraph::setPen) will be used by the scatter points. This makes + it very convenient to set up typical scatter settings: + + \snippet documentation/doc-code-snippets/mainwindow.cpp qcpscatterstyle-shortcreation + + Notice that it wasn't even necessary to explicitly call a QCPScatterStyle constructor. This works + because QCPScatterStyle provides a constructor that can transform a \ref ScatterShape directly + into a QCPScatterStyle instance (that's the \ref QCPScatterStyle(ScatterShape shape, double size) + constructor with a default for \a size). In those cases, C++ allows directly supplying a \ref + ScatterShape, where actually a QCPScatterStyle is expected. + + \section QCPScatterStyle-custompath-and-pixmap Custom shapes and pixmaps + + QCPScatterStyle supports drawing custom shapes and arbitrary pixmaps as scatter points. + + For custom shapes, you can provide a QPainterPath with the desired shape to the \ref + setCustomPath function or call the constructor that takes a painter path. The scatter shape will + automatically be set to \ref ssCustom. + + For pixmaps, you call \ref setPixmap with the desired QPixmap. Alternatively you can use the + constructor that takes a QPixmap. The scatter shape will automatically be set to \ref ssPixmap. + Note that \ref setSize does not influence the appearance of the pixmap. +*/ + +/* start documentation of inline functions */ + +/*! \fn bool QCPScatterStyle::isNone() const + + Returns whether the scatter shape is \ref ssNone. + + \see setShape +*/ + +/*! \fn bool QCPScatterStyle::isPenDefined() const + + Returns whether a pen has been defined for this scatter style. + + The pen is undefined if a constructor is called that does not carry \a pen as parameter. Those are + \ref QCPScatterStyle() and \ref QCPScatterStyle(ScatterShape shape, double size). If the pen is + left undefined, the scatter color will be inherited from the plottable that uses this scatter + style. + + \see setPen +*/ + +/* end documentation of inline functions */ + +/*! + Creates a new QCPScatterStyle instance with size set to 6. No shape, pen or brush is defined. + + Since the pen is undefined (\ref isPenDefined returns false), the scatter color will be inherited + from the plottable that uses this scatter style. +*/ +QCPScatterStyle::QCPScatterStyle() : + mSize(6), + mShape(ssNone), + mPen(Qt::NoPen), + mBrush(Qt::NoBrush), + mPenDefined(false) +{ +} + +/*! + Creates a new QCPScatterStyle instance with shape set to \a shape and size to \a size. No pen or + brush is defined. + + Since the pen is undefined (\ref isPenDefined returns false), the scatter color will be inherited + from the plottable that uses this scatter style. +*/ +QCPScatterStyle::QCPScatterStyle(ScatterShape shape, double size) : + mSize(size), + mShape(shape), + mPen(Qt::NoPen), + mBrush(Qt::NoBrush), + mPenDefined(false) +{ +} + +/*! + Creates a new QCPScatterStyle instance with shape set to \a shape, the pen color set to \a color, + and size to \a size. No brush is defined, i.e. the scatter point will not be filled. +*/ +QCPScatterStyle::QCPScatterStyle(ScatterShape shape, const QColor &color, double size) : + mSize(size), + mShape(shape), + mPen(QPen(color)), + mBrush(Qt::NoBrush), + mPenDefined(true) +{ +} + +/*! + Creates a new QCPScatterStyle instance with shape set to \a shape, the pen color set to \a color, + the brush color to \a fill (with a solid pattern), and size to \a size. +*/ +QCPScatterStyle::QCPScatterStyle(ScatterShape shape, const QColor &color, const QColor &fill, double size) : + mSize(size), + mShape(shape), + mPen(QPen(color)), + mBrush(QBrush(fill)), + mPenDefined(true) +{ +} + +/*! + Creates a new QCPScatterStyle instance with shape set to \a shape, the pen set to \a pen, the + brush to \a brush, and size to \a size. + + \warning In some cases it might be tempting to directly use a pen style like Qt::NoPen as \a pen + and a color like Qt::blue as \a brush. Notice however, that the corresponding call\n + QCPScatterStyle(QCPScatterShape::ssCircle, Qt::NoPen, Qt::blue, 5)\n + doesn't necessarily lead C++ to use this constructor in some cases, but might mistake + Qt::NoPen for a QColor and use the + \ref QCPScatterStyle(ScatterShape shape, const QColor &color, const QColor &fill, double size) + constructor instead (which will lead to an unexpected look of the scatter points). To prevent + this, be more explicit with the parameter types. For example, use QBrush(Qt::blue) + instead of just Qt::blue, to clearly point out to the compiler that this constructor is + wanted. +*/ +QCPScatterStyle::QCPScatterStyle(ScatterShape shape, const QPen &pen, const QBrush &brush, double size) : + mSize(size), + mShape(shape), + mPen(pen), + mBrush(brush), + mPenDefined(pen.style() != Qt::NoPen) +{ +} + +/*! + Creates a new QCPScatterStyle instance which will show the specified \a pixmap. The scatter shape + is set to \ref ssPixmap. +*/ +QCPScatterStyle::QCPScatterStyle(const QPixmap &pixmap) : + mSize(5), + mShape(ssPixmap), + mPen(Qt::NoPen), + mBrush(Qt::NoBrush), + mPixmap(pixmap), + mPenDefined(false) +{ +} + +/*! + Creates a new QCPScatterStyle instance with a custom shape that is defined via \a customPath. The + scatter shape is set to \ref ssCustom. + + The custom shape line will be drawn with \a pen and filled with \a brush. The size has a slightly + different meaning than for built-in scatter points: The custom path will be drawn scaled by a + factor of \a size/6.0. Since the default \a size is 6, the custom path will appear at a its + natural size by default. To double the size of the path for example, set \a size to 12. +*/ +QCPScatterStyle::QCPScatterStyle(const QPainterPath &customPath, const QPen &pen, const QBrush &brush, double size) : + mSize(size), + mShape(ssCustom), + mPen(pen), + mBrush(brush), + mCustomPath(customPath), + mPenDefined(pen.style() != Qt::NoPen) +{ +} + +/*! + Sets the size (pixel diameter) of the drawn scatter points to \a size. + + \see setShape +*/ +void QCPScatterStyle::setSize(double size) +{ + mSize = size; +} + +/*! + Sets the shape to \a shape. + + Note that the calls \ref setPixmap and \ref setCustomPath automatically set the shape to \ref + ssPixmap and \ref ssCustom, respectively. + + \see setSize +*/ +void QCPScatterStyle::setShape(QCPScatterStyle::ScatterShape shape) +{ + mShape = shape; +} + +/*! + Sets the pen that will be used to draw scatter points to \a pen. + + If the pen was previously undefined (see \ref isPenDefined), the pen is considered defined after + a call to this function, even if \a pen is Qt::NoPen. + + \see setBrush +*/ +void QCPScatterStyle::setPen(const QPen &pen) +{ + mPenDefined = true; + mPen = pen; +} + +/*! + Sets the brush that will be used to fill scatter points to \a brush. Note that not all scatter + shapes have fillable areas. For example, \ref ssPlus does not while \ref ssCircle does. + + \see setPen +*/ +void QCPScatterStyle::setBrush(const QBrush &brush) +{ + mBrush = brush; +} + +/*! + Sets the pixmap that will be drawn as scatter point to \a pixmap. + + Note that \ref setSize does not influence the appearance of the pixmap. + + The scatter shape is automatically set to \ref ssPixmap. +*/ +void QCPScatterStyle::setPixmap(const QPixmap &pixmap) +{ + setShape(ssPixmap); + mPixmap = pixmap; +} + +/*! + Sets the custom shape that will be drawn as scatter point to \a customPath. + + The scatter shape is automatically set to \ref ssCustom. +*/ +void QCPScatterStyle::setCustomPath(const QPainterPath &customPath) +{ + setShape(ssCustom); + mCustomPath = customPath; +} + +/*! + Applies the pen and the brush of this scatter style to \a painter. If this scatter style has an + undefined pen (\ref isPenDefined), sets the pen of \a painter to \a defaultPen instead. + + This function is used by plottables (or any class that wants to draw scatters) just before a + number of scatters with this style shall be drawn with the \a painter. + + \see drawShape +*/ +void QCPScatterStyle::applyTo(QCPPainter *painter, const QPen &defaultPen) const +{ + painter->setPen(mPenDefined ? mPen : defaultPen); + painter->setBrush(mBrush); +} + +/*! + Draws the scatter shape with \a painter at position \a pos. + + This function does not modify the pen or the brush on the painter, as \ref applyTo is meant to be + called before scatter points are drawn with \ref drawShape. + + \see applyTo +*/ +void QCPScatterStyle::drawShape(QCPPainter *painter, QPointF pos) const +{ + drawShape(painter, pos.x(), pos.y()); +} + +/*! \overload + Draws the scatter shape with \a painter at position \a x and \a y. +*/ +void QCPScatterStyle::drawShape(QCPPainter *painter, double x, double y) const +{ + double w = mSize/2.0; + switch (mShape) + { + case ssNone: break; + case ssDot: + { + painter->drawLine(QPointF(x, y), QPointF(x+0.0001, y)); + break; + } + case ssCross: + { + painter->drawLine(QLineF(x-w, y-w, x+w, y+w)); + painter->drawLine(QLineF(x-w, y+w, x+w, y-w)); + break; + } + case ssPlus: + { + painter->drawLine(QLineF(x-w, y, x+w, y)); + painter->drawLine(QLineF( x, y+w, x, y-w)); + break; + } + case ssCircle: + { + painter->drawEllipse(QPointF(x , y), w, w); + break; + } + case ssDisc: + { + QBrush b = painter->brush(); + painter->setBrush(painter->pen().color()); + painter->drawEllipse(QPointF(x , y), w, w); + painter->setBrush(b); + break; + } + case ssSquare: + { + painter->drawRect(QRectF(x-w, y-w, mSize, mSize)); + break; + } + case ssDiamond: + { + painter->drawLine(QLineF(x-w, y, x, y-w)); + painter->drawLine(QLineF( x, y-w, x+w, y)); + painter->drawLine(QLineF(x+w, y, x, y+w)); + painter->drawLine(QLineF( x, y+w, x-w, y)); + break; + } + case ssStar: + { + painter->drawLine(QLineF(x-w, y, x+w, y)); + painter->drawLine(QLineF( x, y+w, x, y-w)); + painter->drawLine(QLineF(x-w*0.707, y-w*0.707, x+w*0.707, y+w*0.707)); + painter->drawLine(QLineF(x-w*0.707, y+w*0.707, x+w*0.707, y-w*0.707)); + break; + } + case ssTriangle: + { + painter->drawLine(QLineF(x-w, y+0.755*w, x+w, y+0.755*w)); + painter->drawLine(QLineF(x+w, y+0.755*w, x, y-0.977*w)); + painter->drawLine(QLineF( x, y-0.977*w, x-w, y+0.755*w)); + break; + } + case ssTriangleInverted: + { + painter->drawLine(QLineF(x-w, y-0.755*w, x+w, y-0.755*w)); + painter->drawLine(QLineF(x+w, y-0.755*w, x, y+0.977*w)); + painter->drawLine(QLineF( x, y+0.977*w, x-w, y-0.755*w)); + break; + } + case ssCrossSquare: + { + painter->drawLine(QLineF(x-w, y-w, x+w*0.95, y+w*0.95)); + painter->drawLine(QLineF(x-w, y+w*0.95, x+w*0.95, y-w)); + painter->drawRect(QRectF(x-w, y-w, mSize, mSize)); + break; + } + case ssPlusSquare: + { + painter->drawLine(QLineF(x-w, y, x+w*0.95, y)); + painter->drawLine(QLineF( x, y+w, x, y-w)); + painter->drawRect(QRectF(x-w, y-w, mSize, mSize)); + break; + } + case ssCrossCircle: + { + painter->drawLine(QLineF(x-w*0.707, y-w*0.707, x+w*0.670, y+w*0.670)); + painter->drawLine(QLineF(x-w*0.707, y+w*0.670, x+w*0.670, y-w*0.707)); + painter->drawEllipse(QPointF(x, y), w, w); + break; + } + case ssPlusCircle: + { + painter->drawLine(QLineF(x-w, y, x+w, y)); + painter->drawLine(QLineF( x, y+w, x, y-w)); + painter->drawEllipse(QPointF(x, y), w, w); + break; + } + case ssPeace: + { + painter->drawLine(QLineF(x, y-w, x, y+w)); + painter->drawLine(QLineF(x, y, x-w*0.707, y+w*0.707)); + painter->drawLine(QLineF(x, y, x+w*0.707, y+w*0.707)); + painter->drawEllipse(QPointF(x, y), w, w); + break; + } + case ssPixmap: + { + painter->drawPixmap(x-mPixmap.width()*0.5, y-mPixmap.height()*0.5, mPixmap); + break; + } + case ssCustom: + { + QTransform oldTransform = painter->transform(); + painter->translate(x, y); + painter->scale(mSize/6.0, mSize/6.0); + painter->drawPath(mCustomPath); + painter->setTransform(oldTransform); + break; + } + } +} + + +//////////////////////////////////////////////////////////////////////////////////////////////////// +//////////////////// QCPLayer +//////////////////////////////////////////////////////////////////////////////////////////////////// + +/*! \class QCPLayer + \brief A layer that may contain objects, to control the rendering order + + The Layering system of QCustomPlot is the mechanism to control the rendering order of the + elements inside the plot. + + It is based on the two classes QCPLayer and QCPLayerable. QCustomPlot holds an ordered list of + one or more instances of QCPLayer (see QCustomPlot::addLayer, QCustomPlot::layer, + QCustomPlot::moveLayer, etc.). When replotting, QCustomPlot goes through the list of layers + bottom to top and successively draws the layerables of the layers. + + A QCPLayer contains an ordered list of QCPLayerable instances. QCPLayerable is an abstract base + class from which almost all visible objects derive, like axes, grids, graphs, items, etc. + + Initially, QCustomPlot has five layers: "background", "grid", "main", "axes" and "legend" (in + that order). The top two layers "axes" and "legend" contain the default axes and legend, so they + will be drawn on top. In the middle, there is the "main" layer. It is initially empty and set as + the current layer (see QCustomPlot::setCurrentLayer). This means, all new plottables, items etc. + are created on this layer by default. Then comes the "grid" layer which contains the QCPGrid + instances (which belong tightly to QCPAxis, see \ref QCPAxis::grid). The Axis rect background + shall be drawn behind everything else, thus the default QCPAxisRect instance is placed on the + "background" layer. Of course, the layer affiliation of the individual objects can be changed as + required (\ref QCPLayerable::setLayer). + + Controlling the ordering of objects is easy: Create a new layer in the position you want it to + be, e.g. above "main", with QCustomPlot::addLayer. Then set the current layer with + QCustomPlot::setCurrentLayer to that new layer and finally create the objects normally. They will + be placed on the new layer automatically, due to the current layer setting. Alternatively you + could have also ignored the current layer setting and just moved the objects with + QCPLayerable::setLayer to the desired layer after creating them. + + It is also possible to move whole layers. For example, If you want the grid to be shown in front + of all plottables/items on the "main" layer, just move it above "main" with + QCustomPlot::moveLayer. + + The rendering order within one layer is simply by order of creation or insertion. The item + created last (or added last to the layer), is drawn on top of all other objects on that layer. + + When a layer is deleted, the objects on it are not deleted with it, but fall on the layer below + the deleted layer, see QCustomPlot::removeLayer. +*/ + +/* start documentation of inline functions */ + +/*! \fn QList QCPLayer::children() const + + Returns a list of all layerables on this layer. The order corresponds to the rendering order: + layerables with higher indices are drawn above layerables with lower indices. +*/ + +/*! \fn int QCPLayer::index() const + + Returns the index this layer has in the QCustomPlot. The index is the integer number by which this layer can be + accessed via \ref QCustomPlot::layer. + + Layers with higher indices will be drawn above layers with lower indices. +*/ + +/* end documentation of inline functions */ + +/*! + Creates a new QCPLayer instance. + + Normally you shouldn't directly instantiate layers, use \ref QCustomPlot::addLayer instead. + + \warning It is not checked that \a layerName is actually a unique layer name in \a parentPlot. + This check is only performed by \ref QCustomPlot::addLayer. +*/ +QCPLayer::QCPLayer(QCustomPlot *parentPlot, const QString &layerName) : + QObject(parentPlot), + mParentPlot(parentPlot), + mName(layerName), + mIndex(-1), // will be set to a proper value by the QCustomPlot layer creation function + mVisible(true) +{ + // Note: no need to make sure layerName is unique, because layer + // management is done with QCustomPlot functions. +} + +QCPLayer::~QCPLayer() +{ + // If child layerables are still on this layer, detach them, so they don't try to reach back to this + // then invalid layer once they get deleted/moved themselves. This only happens when layers are deleted + // directly, like in the QCustomPlot destructor. (The regular layer removal procedure for the user is to + // call QCustomPlot::removeLayer, which moves all layerables off this layer before deleting it.) + + while (!mChildren.isEmpty()) + mChildren.last()->setLayer(0); // removes itself from mChildren via removeChild() + + if (mParentPlot->currentLayer() == this) + qDebug() << Q_FUNC_INFO << "The parent plot's mCurrentLayer will be a dangling pointer. Should have been set to a valid layer or 0 beforehand."; +} + +/*! + Sets whether this layer is visible or not. If \a visible is set to false, all layerables on this + layer will be invisible. + + This function doesn't change the visibility property of the layerables (\ref + QCPLayerable::setVisible), but the \ref QCPLayerable::realVisibility of each layerable takes the + visibility of the parent layer into account. +*/ +void QCPLayer::setVisible(bool visible) +{ + mVisible = visible; +} + +/*! \internal + + Adds the \a layerable to the list of this layer. If \a prepend is set to true, the layerable will + be prepended to the list, i.e. be drawn beneath the other layerables already in the list. + + This function does not change the \a mLayer member of \a layerable to this layer. (Use + QCPLayerable::setLayer to change the layer of an object, not this function.) + + \see removeChild +*/ +void QCPLayer::addChild(QCPLayerable *layerable, bool prepend) +{ + if (!mChildren.contains(layerable)) + { + if (prepend) + mChildren.prepend(layerable); + else + mChildren.append(layerable); + } else + qDebug() << Q_FUNC_INFO << "layerable is already child of this layer" << reinterpret_cast(layerable); +} + +/*! \internal + + Removes the \a layerable from the list of this layer. + + This function does not change the \a mLayer member of \a layerable. (Use QCPLayerable::setLayer + to change the layer of an object, not this function.) + + \see addChild +*/ +void QCPLayer::removeChild(QCPLayerable *layerable) +{ + if (!mChildren.removeOne(layerable)) + qDebug() << Q_FUNC_INFO << "layerable is not child of this layer" << reinterpret_cast(layerable); +} + + +//////////////////////////////////////////////////////////////////////////////////////////////////// +//////////////////// QCPLayerable +//////////////////////////////////////////////////////////////////////////////////////////////////// + +/*! \class QCPLayerable + \brief Base class for all drawable objects + + This is the abstract base class most visible objects derive from, e.g. plottables, axes, grid + etc. + + Every layerable is on a layer (QCPLayer) which allows controlling the rendering order by stacking + the layers accordingly. + + For details about the layering mechanism, see the QCPLayer documentation. +*/ + +/* start documentation of inline functions */ + +/*! \fn QCPLayerable *QCPLayerable::parentLayerable() const + + Returns the parent layerable of this layerable. The parent layerable is used to provide + visibility hierarchies in conjunction with the method \ref realVisibility. This way, layerables + only get drawn if their parent layerables are visible, too. + + Note that a parent layerable is not necessarily also the QObject parent for memory management. + Further, a layerable doesn't always have a parent layerable, so this function may return 0. + + A parent layerable is set implicitly with when placed inside layout elements and doesn't need to be + set manually by the user. +*/ + +/* end documentation of inline functions */ +/* start documentation of pure virtual functions */ + +/*! \fn virtual void QCPLayerable::applyDefaultAntialiasingHint(QCPPainter *painter) const = 0 + \internal + + This function applies the default antialiasing setting to the specified \a painter, using the + function \ref applyAntialiasingHint. It is the antialiasing state the painter is put in, when + \ref draw is called on the layerable. If the layerable has multiple entities whose antialiasing + setting may be specified individually, this function should set the antialiasing state of the + most prominent entity. In this case however, the \ref draw function usually calls the specialized + versions of this function before drawing each entity, effectively overriding the setting of the + default antialiasing hint. + + First example: QCPGraph has multiple entities that have an antialiasing setting: The graph + line, fills, scatters and error bars. Those can be configured via QCPGraph::setAntialiased, + QCPGraph::setAntialiasedFill, QCPGraph::setAntialiasedScatters etc. Consequently, there isn't + only the QCPGraph::applyDefaultAntialiasingHint function (which corresponds to the graph line's + antialiasing), but specialized ones like QCPGraph::applyFillAntialiasingHint and + QCPGraph::applyScattersAntialiasingHint. So before drawing one of those entities, QCPGraph::draw + calls the respective specialized applyAntialiasingHint function. + + Second example: QCPItemLine consists only of a line so there is only one antialiasing + setting which can be controlled with QCPItemLine::setAntialiased. (This function is inherited by + all layerables. The specialized functions, as seen on QCPGraph, must be added explicitly to the + respective layerable subclass.) Consequently it only has the normal + QCPItemLine::applyDefaultAntialiasingHint. The \ref QCPItemLine::draw function doesn't need to + care about setting any antialiasing states, because the default antialiasing hint is already set + on the painter when the \ref draw function is called, and that's the state it wants to draw the + line with. +*/ + +/*! \fn virtual void QCPLayerable::draw(QCPPainter *painter) const = 0 + \internal + + This function draws the layerable with the specified \a painter. It is only called by + QCustomPlot, if the layerable is visible (\ref setVisible). + + Before this function is called, the painter's antialiasing state is set via \ref + applyDefaultAntialiasingHint, see the documentation there. Further, the clipping rectangle was + set to \ref clipRect. +*/ + +/* end documentation of pure virtual functions */ +/* start documentation of signals */ + +/*! \fn void QCPLayerable::layerChanged(QCPLayer *newLayer); + + This signal is emitted when the layer of this layerable changes, i.e. this layerable is moved to + a different layer. + + \see setLayer +*/ + +/* end documentation of signals */ + +/*! + Creates a new QCPLayerable instance. + + Since QCPLayerable is an abstract base class, it can't be instantiated directly. Use one of the + derived classes. + + If \a plot is provided, it automatically places itself on the layer named \a targetLayer. If \a + targetLayer is an empty string, it places itself on the current layer of the plot (see \ref + QCustomPlot::setCurrentLayer). + + It is possible to provide 0 as \a plot. In that case, you should assign a parent plot at a later + time with \ref initializeParentPlot. + + The layerable's parent layerable is set to \a parentLayerable, if provided. Direct layerable + parents are mainly used to control visibility in a hierarchy of layerables. This means a + layerable is only drawn, if all its ancestor layerables are also visible. Note that \a + parentLayerable does not become the QObject-parent (for memory management) of this layerable, \a + plot does. It is not uncommon to set the QObject-parent to something else in the constructors of + QCPLayerable subclasses, to guarantee a working destruction hierarchy. +*/ +QCPLayerable::QCPLayerable(QCustomPlot *plot, QString targetLayer, QCPLayerable *parentLayerable) : + QObject(plot), + mVisible(true), + mParentPlot(plot), + mParentLayerable(parentLayerable), + mLayer(0), + mAntialiased(true) +{ + if (mParentPlot) + { + if (targetLayer.isEmpty()) + setLayer(mParentPlot->currentLayer()); + else if (!setLayer(targetLayer)) + qDebug() << Q_FUNC_INFO << "setting QCPlayerable initial layer to" << targetLayer << "failed."; + } +} + +QCPLayerable::~QCPLayerable() +{ + if (mLayer) + { + mLayer->removeChild(this); + mLayer = 0; + } +} + +/*! + Sets the visibility of this layerable object. If an object is not visible, it will not be drawn + on the QCustomPlot surface, and user interaction with it (e.g. click and selection) is not + possible. +*/ +void QCPLayerable::setVisible(bool on) +{ + mVisible = on; +} + +/*! + Sets the \a layer of this layerable object. The object will be placed on top of the other objects + already on \a layer. + + If \a layer is 0, this layerable will not be on any layer and thus not appear in the plot (or + interact/receive events). + + Returns true if the layer of this layerable was successfully changed to \a layer. +*/ +bool QCPLayerable::setLayer(QCPLayer *layer) +{ + return moveToLayer(layer, false); +} + +/*! \overload + Sets the layer of this layerable object by name + + Returns true on success, i.e. if \a layerName is a valid layer name. +*/ +bool QCPLayerable::setLayer(const QString &layerName) +{ + if (!mParentPlot) + { + qDebug() << Q_FUNC_INFO << "no parent QCustomPlot set"; + return false; + } + if (QCPLayer *layer = mParentPlot->layer(layerName)) + { + return setLayer(layer); + } else + { + qDebug() << Q_FUNC_INFO << "there is no layer with name" << layerName; + return false; + } +} + +/*! + Sets whether this object will be drawn antialiased or not. + + Note that antialiasing settings may be overridden by QCustomPlot::setAntialiasedElements and + QCustomPlot::setNotAntialiasedElements. +*/ +void QCPLayerable::setAntialiased(bool enabled) +{ + mAntialiased = enabled; +} + +/*! + Returns whether this layerable is visible, taking the visibility of the layerable parent and the + visibility of the layer this layerable is on into account. This is the method that is consulted + to decide whether a layerable shall be drawn or not. + + If this layerable has a direct layerable parent (usually set via hierarchies implemented in + subclasses, like in the case of QCPLayoutElement), this function returns true only if this + layerable has its visibility set to true and the parent layerable's \ref realVisibility returns + true. + + If this layerable doesn't have a direct layerable parent, returns the state of this layerable's + visibility. +*/ +bool QCPLayerable::realVisibility() const +{ + return mVisible && (!mLayer || mLayer->visible()) && (!mParentLayerable || mParentLayerable.data()->realVisibility()); +} + +/*! + This function is used to decide whether a click hits a layerable object or not. + + \a pos is a point in pixel coordinates on the QCustomPlot surface. This function returns the + shortest pixel distance of this point to the object. If the object is either invisible or the + distance couldn't be determined, -1.0 is returned. Further, if \a onlySelectable is true and the + object is not selectable, -1.0 is returned, too. + + If the object is represented not by single lines but by an area like a \ref QCPItemText or the + bars of a \ref QCPBars plottable, a click inside the area should also be considered a hit. In + these cases this function thus returns a constant value greater zero but still below the parent + plot's selection tolerance. (typically the selectionTolerance multiplied by 0.99). + + Providing a constant value for area objects allows selecting line objects even when they are + obscured by such area objects, by clicking close to the lines (i.e. closer than + 0.99*selectionTolerance). + + The actual setting of the selection state is not done by this function. This is handled by the + parent QCustomPlot when the mouseReleaseEvent occurs, and the finally selected object is notified + via the selectEvent/deselectEvent methods. + + \a details is an optional output parameter. Every layerable subclass may place any information + in \a details. This information will be passed to \ref selectEvent when the parent QCustomPlot + decides on the basis of this selectTest call, that the object was successfully selected. The + subsequent call to \ref selectEvent will carry the \a details. This is useful for multi-part + objects (like QCPAxis). This way, a possibly complex calculation to decide which part was clicked + is only done once in \ref selectTest. The result (i.e. the actually clicked part) can then be + placed in \a details. So in the subsequent \ref selectEvent, the decision which part was + selected doesn't have to be done a second time for a single selection operation. + + You may pass 0 as \a details to indicate that you are not interested in those selection details. + + \see selectEvent, deselectEvent, QCustomPlot::setInteractions +*/ +double QCPLayerable::selectTest(const QPointF &pos, bool onlySelectable, QVariant *details) const +{ + Q_UNUSED(pos) + Q_UNUSED(onlySelectable) + Q_UNUSED(details) + return -1.0; +} + +/*! \internal + + Sets the parent plot of this layerable. Use this function once to set the parent plot if you have + passed 0 in the constructor. It can not be used to move a layerable from one QCustomPlot to + another one. + + Note that, unlike when passing a non-null parent plot in the constructor, this function does not + make \a parentPlot the QObject-parent of this layerable. If you want this, call + QObject::setParent(\a parentPlot) in addition to this function. + + Further, you will probably want to set a layer (\ref setLayer) after calling this function, to + make the layerable appear on the QCustomPlot. + + The parent plot change will be propagated to subclasses via a call to \ref parentPlotInitialized + so they can react accordingly (e.g. also initialize the parent plot of child layerables, like + QCPLayout does). +*/ +void QCPLayerable::initializeParentPlot(QCustomPlot *parentPlot) +{ + if (mParentPlot) + { + qDebug() << Q_FUNC_INFO << "called with mParentPlot already initialized"; + return; + } + + if (!parentPlot) + qDebug() << Q_FUNC_INFO << "called with parentPlot zero"; + + mParentPlot = parentPlot; + parentPlotInitialized(mParentPlot); +} + +/*! \internal + + Sets the parent layerable of this layerable to \a parentLayerable. Note that \a parentLayerable does not + become the QObject-parent (for memory management) of this layerable. + + The parent layerable has influence on the return value of the \ref realVisibility method. Only + layerables with a fully visible parent tree will return true for \ref realVisibility, and thus be + drawn. + + \see realVisibility +*/ +void QCPLayerable::setParentLayerable(QCPLayerable *parentLayerable) +{ + mParentLayerable = parentLayerable; +} + +/*! \internal + + Moves this layerable object to \a layer. If \a prepend is true, this object will be prepended to + the new layer's list, i.e. it will be drawn below the objects already on the layer. If it is + false, the object will be appended. + + Returns true on success, i.e. if \a layer is a valid layer. +*/ +bool QCPLayerable::moveToLayer(QCPLayer *layer, bool prepend) +{ + if (layer && !mParentPlot) + { + qDebug() << Q_FUNC_INFO << "no parent QCustomPlot set"; + return false; + } + if (layer && layer->parentPlot() != mParentPlot) + { + qDebug() << Q_FUNC_INFO << "layer" << layer->name() << "is not in same QCustomPlot as this layerable"; + return false; + } + + QCPLayer *oldLayer = mLayer; + if (mLayer) + mLayer->removeChild(this); + mLayer = layer; + if (mLayer) + mLayer->addChild(this, prepend); + if (mLayer != oldLayer) + emit layerChanged(mLayer); + return true; +} + +/*! \internal + + Sets the QCPainter::setAntialiasing state on the provided \a painter, depending on the \a + localAntialiased value as well as the overrides \ref QCustomPlot::setAntialiasedElements and \ref + QCustomPlot::setNotAntialiasedElements. Which override enum this function takes into account is + controlled via \a overrideElement. +*/ +void QCPLayerable::applyAntialiasingHint(QCPPainter *painter, bool localAntialiased, QCP::AntialiasedElement overrideElement) const +{ + if (mParentPlot && mParentPlot->notAntialiasedElements().testFlag(overrideElement)) + painter->setAntialiasing(false); + else if (mParentPlot && mParentPlot->antialiasedElements().testFlag(overrideElement)) + painter->setAntialiasing(true); + else + painter->setAntialiasing(localAntialiased); +} + +/*! \internal + + This function is called by \ref initializeParentPlot, to allow subclasses to react on the setting + of a parent plot. This is the case when 0 was passed as parent plot in the constructor, and the + parent plot is set at a later time. + + For example, QCPLayoutElement/QCPLayout hierarchies may be created independently of any + QCustomPlot at first. When they are then added to a layout inside the QCustomPlot, the top level + element of the hierarchy gets its parent plot initialized with \ref initializeParentPlot. To + propagate the parent plot to all the children of the hierarchy, the top level element then uses + this function to pass the parent plot on to its child elements. + + The default implementation does nothing. + + \see initializeParentPlot +*/ +void QCPLayerable::parentPlotInitialized(QCustomPlot *parentPlot) +{ + Q_UNUSED(parentPlot) +} + +/*! \internal + + Returns the selection category this layerable shall belong to. The selection category is used in + conjunction with \ref QCustomPlot::setInteractions to control which objects are selectable and + which aren't. + + Subclasses that don't fit any of the normal \ref QCP::Interaction values can use \ref + QCP::iSelectOther. This is what the default implementation returns. + + \see QCustomPlot::setInteractions +*/ +QCP::Interaction QCPLayerable::selectionCategory() const +{ + return QCP::iSelectOther; +} + +/*! \internal + + Returns the clipping rectangle of this layerable object. By default, this is the viewport of the + parent QCustomPlot. Specific subclasses may reimplement this function to provide different + clipping rects. + + The returned clipping rect is set on the painter before the draw function of the respective + object is called. +*/ +QRect QCPLayerable::clipRect() const +{ + if (mParentPlot) + return mParentPlot->viewport(); + else + return QRect(); +} + +/*! \internal + + This event is called when the layerable shall be selected, as a consequence of a click by the + user. Subclasses should react to it by setting their selection state appropriately. The default + implementation does nothing. + + \a event is the mouse event that caused the selection. \a additive indicates, whether the user + was holding the multi-select-modifier while performing the selection (see \ref + QCustomPlot::setMultiSelectModifier). if \a additive is true, the selection state must be toggled + (i.e. become selected when unselected and unselected when selected). + + Every selectEvent is preceded by a call to \ref selectTest, which has returned positively (i.e. + returned a value greater than 0 and less than the selection tolerance of the parent QCustomPlot). + The \a details data you output from \ref selectTest is fed back via \a details here. You may + use it to transport any kind of information from the selectTest to the possibly subsequent + selectEvent. Usually \a details is used to transfer which part was clicked, if it is a layerable + that has multiple individually selectable parts (like QCPAxis). This way selectEvent doesn't need + to do the calculation again to find out which part was actually clicked. + + \a selectionStateChanged is an output parameter. If the pointer is non-null, this function must + set the value either to true or false, depending on whether the selection state of this layerable + was actually changed. For layerables that only are selectable as a whole and not in parts, this + is simple: if \a additive is true, \a selectionStateChanged must also be set to true, because the + selection toggles. If \a additive is false, \a selectionStateChanged is only set to true, if the + layerable was previously unselected and now is switched to the selected state. + + \see selectTest, deselectEvent +*/ +void QCPLayerable::selectEvent(QMouseEvent *event, bool additive, const QVariant &details, bool *selectionStateChanged) +{ + Q_UNUSED(event) + Q_UNUSED(additive) + Q_UNUSED(details) + Q_UNUSED(selectionStateChanged) +} + +/*! \internal + + This event is called when the layerable shall be deselected, either as consequence of a user + interaction or a call to \ref QCustomPlot::deselectAll. Subclasses should react to it by + unsetting their selection appropriately. + + just as in \ref selectEvent, the output parameter \a selectionStateChanged (if non-null), must + return true or false when the selection state of this layerable has changed or not changed, + respectively. + + \see selectTest, selectEvent +*/ +void QCPLayerable::deselectEvent(bool *selectionStateChanged) +{ + Q_UNUSED(selectionStateChanged) +} + + +//////////////////////////////////////////////////////////////////////////////////////////////////// +//////////////////// QCPRange +//////////////////////////////////////////////////////////////////////////////////////////////////// +/*! \class QCPRange + \brief Represents the range an axis is encompassing. + + contains a \a lower and \a upper double value and provides convenience input, output and + modification functions. + + \see QCPAxis::setRange +*/ + +/*! + Minimum range size (\a upper - \a lower) the range changing functions will accept. Smaller + intervals would cause errors due to the 11-bit exponent of double precision numbers, + corresponding to a minimum magnitude of roughly 1e-308. + \see validRange, maxRange +*/ +const double QCPRange::minRange = 1e-280; + +/*! + Maximum values (negative and positive) the range will accept in range-changing functions. + Larger absolute values would cause errors due to the 11-bit exponent of double precision numbers, + corresponding to a maximum magnitude of roughly 1e308. + Since the number of planck-volumes in the entire visible universe is only ~1e183, this should + be enough. + \see validRange, minRange +*/ +const double QCPRange::maxRange = 1e250; + +/*! + Constructs a range with \a lower and \a upper set to zero. +*/ +QCPRange::QCPRange() : + lower(0), + upper(0) +{ +} + +/*! \overload + Constructs a range with the specified \a lower and \a upper values. +*/ +QCPRange::QCPRange(double lower, double upper) : + lower(lower), + upper(upper) +{ + normalize(); +} + +/*! + Returns the size of the range, i.e. \a upper-\a lower +*/ +double QCPRange::size() const +{ + return upper-lower; +} + +/*! + Returns the center of the range, i.e. (\a upper+\a lower)*0.5 +*/ +double QCPRange::center() const +{ + return (upper+lower)*0.5; +} + +/*! + Makes sure \a lower is numerically smaller than \a upper. If this is not the case, the values + are swapped. +*/ +void QCPRange::normalize() +{ + if (lower > upper) + qSwap(lower, upper); +} + +/*! + Expands this range such that \a otherRange is contained in the new range. It is assumed that both + this range and \a otherRange are normalized (see \ref normalize). + + If \a otherRange is already inside the current range, this function does nothing. + + \see expanded +*/ +void QCPRange::expand(const QCPRange &otherRange) +{ + if (lower > otherRange.lower) + lower = otherRange.lower; + if (upper < otherRange.upper) + upper = otherRange.upper; +} + + +/*! + Returns an expanded range that contains this and \a otherRange. It is assumed that both this + range and \a otherRange are normalized (see \ref normalize). + + \see expand +*/ +QCPRange QCPRange::expanded(const QCPRange &otherRange) const +{ + QCPRange result = *this; + result.expand(otherRange); + return result; +} + +/*! + Returns a sanitized version of the range. Sanitized means for logarithmic scales, that + the range won't span the positive and negative sign domain, i.e. contain zero. Further + \a lower will always be numerically smaller (or equal) to \a upper. + + If the original range does span positive and negative sign domains or contains zero, + the returned range will try to approximate the original range as good as possible. + If the positive interval of the original range is wider than the negative interval, the + returned range will only contain the positive interval, with lower bound set to \a rangeFac or + \a rangeFac *\a upper, whichever is closer to zero. Same procedure is used if the negative interval + is wider than the positive interval, this time by changing the \a upper bound. +*/ +QCPRange QCPRange::sanitizedForLogScale() const +{ + double rangeFac = 1e-3; + QCPRange sanitizedRange(lower, upper); + sanitizedRange.normalize(); + // can't have range spanning negative and positive values in log plot, so change range to fix it + //if (qFuzzyCompare(sanitizedRange.lower+1, 1) && !qFuzzyCompare(sanitizedRange.upper+1, 1)) + if (sanitizedRange.lower == 0.0 && sanitizedRange.upper != 0.0) + { + // case lower is 0 + if (rangeFac < sanitizedRange.upper*rangeFac) + sanitizedRange.lower = rangeFac; + else + sanitizedRange.lower = sanitizedRange.upper*rangeFac; + } //else if (!qFuzzyCompare(lower+1, 1) && qFuzzyCompare(upper+1, 1)) + else if (sanitizedRange.lower != 0.0 && sanitizedRange.upper == 0.0) + { + // case upper is 0 + if (-rangeFac > sanitizedRange.lower*rangeFac) + sanitizedRange.upper = -rangeFac; + else + sanitizedRange.upper = sanitizedRange.lower*rangeFac; + } else if (sanitizedRange.lower < 0 && sanitizedRange.upper > 0) + { + // find out whether negative or positive interval is wider to decide which sign domain will be chosen + if (-sanitizedRange.lower > sanitizedRange.upper) + { + // negative is wider, do same as in case upper is 0 + if (-rangeFac > sanitizedRange.lower*rangeFac) + sanitizedRange.upper = -rangeFac; + else + sanitizedRange.upper = sanitizedRange.lower*rangeFac; + } else + { + // positive is wider, do same as in case lower is 0 + if (rangeFac < sanitizedRange.upper*rangeFac) + sanitizedRange.lower = rangeFac; + else + sanitizedRange.lower = sanitizedRange.upper*rangeFac; + } + } + // due to normalization, case lower>0 && upper<0 should never occur, because that implies upper= lower && value <= upper; +} + +/*! + Checks, whether the specified range is within valid bounds, which are defined + as QCPRange::maxRange and QCPRange::minRange. + A valid range means: + \li range bounds within -maxRange and maxRange + \li range size above minRange + \li range size below maxRange +*/ +bool QCPRange::validRange(double lower, double upper) +{ + return (lower > -maxRange && + upper < maxRange && + qAbs(lower-upper) > minRange && + qAbs(lower-upper) < maxRange && + !(lower > 0 && qIsInf(upper/lower)) && + !(upper < 0 && qIsInf(lower/upper))); +} + +/*! + \overload + Checks, whether the specified range is within valid bounds, which are defined + as QCPRange::maxRange and QCPRange::minRange. + A valid range means: + \li range bounds within -maxRange and maxRange + \li range size above minRange + \li range size below maxRange +*/ +bool QCPRange::validRange(const QCPRange &range) +{ + return (range.lower > -maxRange && + range.upper < maxRange && + qAbs(range.lower-range.upper) > minRange && + qAbs(range.lower-range.upper) < maxRange && + !(range.lower > 0 && qIsInf(range.upper/range.lower)) && + !(range.upper < 0 && qIsInf(range.lower/range.upper))); +} + + +//////////////////////////////////////////////////////////////////////////////////////////////////// +//////////////////// QCPMarginGroup +//////////////////////////////////////////////////////////////////////////////////////////////////// + +/*! \class QCPMarginGroup + \brief A margin group allows synchronization of margin sides if working with multiple layout elements. + + QCPMarginGroup allows you to tie a margin side of two or more layout elements together, such that + they will all have the same size, based on the largest required margin in the group. + + \n + \image html QCPMarginGroup.png "Demonstration of QCPMarginGroup" + \n + + In certain situations it is desirable that margins at specific sides are synchronized across + layout elements. For example, if one QCPAxisRect is below another one in a grid layout, it will + provide a cleaner look to the user if the left and right margins of the two axis rects are of the + same size. The left axis of the top axis rect will then be at the same horizontal position as the + left axis of the lower axis rect, making them appear aligned. The same applies for the right + axes. This is what QCPMarginGroup makes possible. + + To add/remove a specific side of a layout element to/from a margin group, use the \ref + QCPLayoutElement::setMarginGroup method. To completely break apart the margin group, either call + \ref clear, or just delete the margin group. + + \section QCPMarginGroup-example Example + + First create a margin group: + \snippet documentation/doc-code-snippets/mainwindow.cpp qcpmargingroup-creation-1 + Then set this group on the layout element sides: + \snippet documentation/doc-code-snippets/mainwindow.cpp qcpmargingroup-creation-2 + Here, we've used the first two axis rects of the plot and synchronized their left margins with + each other and their right margins with each other. +*/ + +/* start documentation of inline functions */ + +/*! \fn QList QCPMarginGroup::elements(QCP::MarginSide side) const + + Returns a list of all layout elements that have their margin \a side associated with this margin + group. +*/ + +/* end documentation of inline functions */ + +/*! + Creates a new QCPMarginGroup instance in \a parentPlot. +*/ +QCPMarginGroup::QCPMarginGroup(QCustomPlot *parentPlot) : + QObject(parentPlot), + mParentPlot(parentPlot) +{ + mChildren.insert(QCP::msLeft, QList()); + mChildren.insert(QCP::msRight, QList()); + mChildren.insert(QCP::msTop, QList()); + mChildren.insert(QCP::msBottom, QList()); +} + +QCPMarginGroup::~QCPMarginGroup() +{ + clear(); +} + +/*! + Returns whether this margin group is empty. If this function returns true, no layout elements use + this margin group to synchronize margin sides. +*/ +bool QCPMarginGroup::isEmpty() const +{ + QHashIterator > it(mChildren); + while (it.hasNext()) + { + it.next(); + if (!it.value().isEmpty()) + return false; + } + return true; +} + +/*! + Clears this margin group. The synchronization of the margin sides that use this margin group is + lifted and they will use their individual margin sizes again. +*/ +void QCPMarginGroup::clear() +{ + // make all children remove themselves from this margin group: + QHashIterator > it(mChildren); + while (it.hasNext()) + { + it.next(); + const QList elements = it.value(); + for (int i=elements.size()-1; i>=0; --i) + elements.at(i)->setMarginGroup(it.key(), 0); // removes itself from mChildren via removeChild + } +} + +/*! \internal + + Returns the synchronized common margin for \a side. This is the margin value that will be used by + the layout element on the respective side, if it is part of this margin group. + + The common margin is calculated by requesting the automatic margin (\ref + QCPLayoutElement::calculateAutoMargin) of each element associated with \a side in this margin + group, and choosing the largest returned value. (QCPLayoutElement::minimumMargins is taken into + account, too.) +*/ +int QCPMarginGroup::commonMargin(QCP::MarginSide side) const +{ + // query all automatic margins of the layout elements in this margin group side and find maximum: + int result = 0; + const QList elements = mChildren.value(side); + for (int i=0; iautoMargins().testFlag(side)) + continue; + int m = qMax(elements.at(i)->calculateAutoMargin(side), QCP::getMarginValue(elements.at(i)->minimumMargins(), side)); + if (m > result) + result = m; + } + return result; +} + +/*! \internal + + Adds \a element to the internal list of child elements, for the margin \a side. + + This function does not modify the margin group property of \a element. +*/ +void QCPMarginGroup::addChild(QCP::MarginSide side, QCPLayoutElement *element) +{ + if (!mChildren[side].contains(element)) + mChildren[side].append(element); + else + qDebug() << Q_FUNC_INFO << "element is already child of this margin group side" << reinterpret_cast(element); +} + +/*! \internal + + Removes \a element from the internal list of child elements, for the margin \a side. + + This function does not modify the margin group property of \a element. +*/ +void QCPMarginGroup::removeChild(QCP::MarginSide side, QCPLayoutElement *element) +{ + if (!mChildren[side].removeOne(element)) + qDebug() << Q_FUNC_INFO << "element is not child of this margin group side" << reinterpret_cast(element); +} + + +//////////////////////////////////////////////////////////////////////////////////////////////////// +//////////////////// QCPLayoutElement +//////////////////////////////////////////////////////////////////////////////////////////////////// + +/*! \class QCPLayoutElement + \brief The abstract base class for all objects that form \ref thelayoutsystem "the layout system". + + This is an abstract base class. As such, it can't be instantiated directly, rather use one of its subclasses. + + A Layout element is a rectangular object which can be placed in layouts. It has an outer rect + (QCPLayoutElement::outerRect) and an inner rect (\ref QCPLayoutElement::rect). The difference + between outer and inner rect is called its margin. The margin can either be set to automatic or + manual (\ref setAutoMargins) on a per-side basis. If a side is set to manual, that margin can be + set explicitly with \ref setMargins and will stay fixed at that value. If it's set to automatic, + the layout element subclass will control the value itself (via \ref calculateAutoMargin). + + Layout elements can be placed in layouts (base class QCPLayout) like QCPLayoutGrid. The top level + layout is reachable via \ref QCustomPlot::plotLayout, and is a \ref QCPLayoutGrid. Since \ref + QCPLayout itself derives from \ref QCPLayoutElement, layouts can be nested. + + Thus in QCustomPlot one can divide layout elements into two categories: The ones that are + invisible by themselves, because they don't draw anything. Their only purpose is to manage the + position and size of other layout elements. This category of layout elements usually use + QCPLayout as base class. Then there is the category of layout elements which actually draw + something. For example, QCPAxisRect, QCPLegend and QCPPlotTitle are of this category. This does + not necessarily mean that the latter category can't have child layout elements. QCPLegend for + instance, actually derives from QCPLayoutGrid and the individual legend items are child layout + elements in the grid layout. +*/ + +/* start documentation of inline functions */ + +/*! \fn QCPLayout *QCPLayoutElement::layout() const + + Returns the parent layout of this layout element. +*/ + +/*! \fn QRect QCPLayoutElement::rect() const + + Returns the inner rect of this layout element. The inner rect is the outer rect (\ref + setOuterRect) shrinked by the margins (\ref setMargins, \ref setAutoMargins). + + In some cases, the area between outer and inner rect is left blank. In other cases the margin + area is used to display peripheral graphics while the main content is in the inner rect. This is + where automatic margin calculation becomes interesting because it allows the layout element to + adapt the margins to the peripheral graphics it wants to draw. For example, \ref QCPAxisRect + draws the axis labels and tick labels in the margin area, thus needs to adjust the margins (if + \ref setAutoMargins is enabled) according to the space required by the labels of the axes. +*/ + +/*! \fn virtual void QCPLayoutElement::mousePressEvent(QMouseEvent *event) + + This event is called, if the mouse was pressed while being inside the outer rect of this layout + element. +*/ + +/*! \fn virtual void QCPLayoutElement::mouseMoveEvent(QMouseEvent *event) + + This event is called, if the mouse is moved inside the outer rect of this layout element. +*/ + +/*! \fn virtual void QCPLayoutElement::mouseReleaseEvent(QMouseEvent *event) + + This event is called, if the mouse was previously pressed inside the outer rect of this layout + element and is now released. +*/ + +/*! \fn virtual void QCPLayoutElement::mouseDoubleClickEvent(QMouseEvent *event) + + This event is called, if the mouse is double-clicked inside the outer rect of this layout + element. +*/ + +/*! \fn virtual void QCPLayoutElement::wheelEvent(QWheelEvent *event) + + This event is called, if the mouse wheel is scrolled while the cursor is inside the rect of this + layout element. +*/ + +/* end documentation of inline functions */ + +/*! + Creates an instance of QCPLayoutElement and sets default values. +*/ +QCPLayoutElement::QCPLayoutElement(QCustomPlot *parentPlot) : + QCPLayerable(parentPlot), // parenthood is changed as soon as layout element gets inserted into a layout (except for top level layout) + mParentLayout(0), + mMinimumSize(), + mMaximumSize(QWIDGETSIZE_MAX, QWIDGETSIZE_MAX), + mRect(0, 0, 0, 0), + mOuterRect(0, 0, 0, 0), + mMargins(0, 0, 0, 0), + mMinimumMargins(0, 0, 0, 0), + mAutoMargins(QCP::msAll) +{ +} + +QCPLayoutElement::~QCPLayoutElement() +{ + setMarginGroup(QCP::msAll, 0); // unregister at margin groups, if there are any + // unregister at layout: + if (qobject_cast(mParentLayout)) // the qobject_cast is just a safeguard in case the layout forgets to call clear() in its dtor and this dtor is called by QObject dtor + mParentLayout->take(this); +} + +/*! + Sets the outer rect of this layout element. If the layout element is inside a layout, the layout + sets the position and size of this layout element using this function. + + Calling this function externally has no effect, since the layout will overwrite any changes to + the outer rect upon the next replot. + + The layout element will adapt its inner \ref rect by applying the margins inward to the outer rect. + + \see rect +*/ +void QCPLayoutElement::setOuterRect(const QRect &rect) +{ + if (mOuterRect != rect) + { + mOuterRect = rect; + mRect = mOuterRect.adjusted(mMargins.left(), mMargins.top(), -mMargins.right(), -mMargins.bottom()); + } +} + +/*! + Sets the margins of this layout element. If \ref setAutoMargins is disabled for some or all + sides, this function is used to manually set the margin on those sides. Sides that are still set + to be handled automatically are ignored and may have any value in \a margins. + + The margin is the distance between the outer rect (controlled by the parent layout via \ref + setOuterRect) and the inner \ref rect (which usually contains the main content of this layout + element). + + \see setAutoMargins +*/ +void QCPLayoutElement::setMargins(const QMargins &margins) +{ + if (mMargins != margins) + { + mMargins = margins; + mRect = mOuterRect.adjusted(mMargins.left(), mMargins.top(), -mMargins.right(), -mMargins.bottom()); + } +} + +/*! + If \ref setAutoMargins is enabled on some or all margins, this function is used to provide + minimum values for those margins. + + The minimum values are not enforced on margin sides that were set to be under manual control via + \ref setAutoMargins. + + \see setAutoMargins +*/ +void QCPLayoutElement::setMinimumMargins(const QMargins &margins) +{ + if (mMinimumMargins != margins) + { + mMinimumMargins = margins; + } +} + +/*! + Sets on which sides the margin shall be calculated automatically. If a side is calculated + automatically, a minimum margin value may be provided with \ref setMinimumMargins. If a side is + set to be controlled manually, the value may be specified with \ref setMargins. + + Margin sides that are under automatic control may participate in a \ref QCPMarginGroup (see \ref + setMarginGroup), to synchronize (align) it with other layout elements in the plot. + + \see setMinimumMargins, setMargins +*/ +void QCPLayoutElement::setAutoMargins(QCP::MarginSides sides) +{ + mAutoMargins = sides; +} + +/*! + Sets the minimum size for the inner \ref rect of this layout element. A parent layout tries to + respect the \a size here by changing row/column sizes in the layout accordingly. + + If the parent layout size is not sufficient to satisfy all minimum size constraints of its child + layout elements, the layout may set a size that is actually smaller than \a size. QCustomPlot + propagates the layout's size constraints to the outside by setting its own minimum QWidget size + accordingly, so violations of \a size should be exceptions. +*/ +void QCPLayoutElement::setMinimumSize(const QSize &size) +{ + if (mMinimumSize != size) + { + mMinimumSize = size; + if (mParentLayout) + mParentLayout->sizeConstraintsChanged(); + } +} + +/*! \overload + + Sets the minimum size for the inner \ref rect of this layout element. +*/ +void QCPLayoutElement::setMinimumSize(int width, int height) +{ + setMinimumSize(QSize(width, height)); +} + +/*! + Sets the maximum size for the inner \ref rect of this layout element. A parent layout tries to + respect the \a size here by changing row/column sizes in the layout accordingly. +*/ +void QCPLayoutElement::setMaximumSize(const QSize &size) +{ + if (mMaximumSize != size) + { + mMaximumSize = size; + if (mParentLayout) + mParentLayout->sizeConstraintsChanged(); + } +} + +/*! \overload + + Sets the maximum size for the inner \ref rect of this layout element. +*/ +void QCPLayoutElement::setMaximumSize(int width, int height) +{ + setMaximumSize(QSize(width, height)); +} + +/*! + Sets the margin \a group of the specified margin \a sides. + + Margin groups allow synchronizing specified margins across layout elements, see the documentation + of \ref QCPMarginGroup. + + To unset the margin group of \a sides, set \a group to 0. + + Note that margin groups only work for margin sides that are set to automatic (\ref + setAutoMargins). +*/ +void QCPLayoutElement::setMarginGroup(QCP::MarginSides sides, QCPMarginGroup *group) +{ + QVector sideVector; + if (sides.testFlag(QCP::msLeft)) sideVector.append(QCP::msLeft); + if (sides.testFlag(QCP::msRight)) sideVector.append(QCP::msRight); + if (sides.testFlag(QCP::msTop)) sideVector.append(QCP::msTop); + if (sides.testFlag(QCP::msBottom)) sideVector.append(QCP::msBottom); + + for (int i=0; iremoveChild(side, this); + + if (!group) // if setting to 0, remove hash entry. Else set hash entry to new group and register there + { + mMarginGroups.remove(side); + } else // setting to a new group + { + mMarginGroups[side] = group; + group->addChild(side, this); + } + } + } +} + +/*! + Updates the layout element and sub-elements. This function is automatically called before every + replot by the parent layout element. It is called multiple times, once for every \ref + UpdatePhase. The phases are run through in the order of the enum values. For details about what + happens at the different phases, see the documentation of \ref UpdatePhase. + + Layout elements that have child elements should call the \ref update method of their child + elements, and pass the current \a phase unchanged. + + The default implementation executes the automatic margin mechanism in the \ref upMargins phase. + Subclasses should make sure to call the base class implementation. +*/ +void QCPLayoutElement::update(UpdatePhase phase) +{ + if (phase == upMargins) + { + if (mAutoMargins != QCP::msNone) + { + // set the margins of this layout element according to automatic margin calculation, either directly or via a margin group: + QMargins newMargins = mMargins; + QList allMarginSides = QList() << QCP::msLeft << QCP::msRight << QCP::msTop << QCP::msBottom; + foreach (QCP::MarginSide side, allMarginSides) + { + if (mAutoMargins.testFlag(side)) // this side's margin shall be calculated automatically + { + if (mMarginGroups.contains(side)) + QCP::setMarginValue(newMargins, side, mMarginGroups[side]->commonMargin(side)); // this side is part of a margin group, so get the margin value from that group + else + QCP::setMarginValue(newMargins, side, calculateAutoMargin(side)); // this side is not part of a group, so calculate the value directly + // apply minimum margin restrictions: + if (QCP::getMarginValue(newMargins, side) < QCP::getMarginValue(mMinimumMargins, side)) + QCP::setMarginValue(newMargins, side, QCP::getMarginValue(mMinimumMargins, side)); + } + } + setMargins(newMargins); + } + } +} + +/*! + Returns the minimum size this layout element (the inner \ref rect) may be compressed to. + + if a minimum size (\ref setMinimumSize) was not set manually, parent layouts consult this + function to determine the minimum allowed size of this layout element. (A manual minimum size is + considered set if it is non-zero.) +*/ +QSize QCPLayoutElement::minimumSizeHint() const +{ + return mMinimumSize; +} + +/*! + Returns the maximum size this layout element (the inner \ref rect) may be expanded to. + + if a maximum size (\ref setMaximumSize) was not set manually, parent layouts consult this + function to determine the maximum allowed size of this layout element. (A manual maximum size is + considered set if it is smaller than Qt's QWIDGETSIZE_MAX.) +*/ +QSize QCPLayoutElement::maximumSizeHint() const +{ + return mMaximumSize; +} + +/*! + Returns a list of all child elements in this layout element. If \a recursive is true, all + sub-child elements are included in the list, too. + + \warning There may be entries with value 0 in the returned list. (For example, QCPLayoutGrid may have + empty cells which yield 0 at the respective index.) +*/ +QList QCPLayoutElement::elements(bool recursive) const +{ + Q_UNUSED(recursive) + return QList(); +} + +/*! + Layout elements are sensitive to events inside their outer rect. If \a pos is within the outer + rect, this method returns a value corresponding to 0.99 times the parent plot's selection + tolerance. However, layout elements are not selectable by default. So if \a onlySelectable is + true, -1.0 is returned. + + See \ref QCPLayerable::selectTest for a general explanation of this virtual method. + + QCPLayoutElement subclasses may reimplement this method to provide more specific selection test + behaviour. +*/ +double QCPLayoutElement::selectTest(const QPointF &pos, bool onlySelectable, QVariant *details) const +{ + Q_UNUSED(details) + + if (onlySelectable) + return -1; + + if (QRectF(mOuterRect).contains(pos)) + { + if (mParentPlot) + return mParentPlot->selectionTolerance()*0.99; + else + { + qDebug() << Q_FUNC_INFO << "parent plot not defined"; + return -1; + } + } else + return -1; +} + +/*! \internal + + propagates the parent plot initialization to all child elements, by calling \ref + QCPLayerable::initializeParentPlot on them. +*/ +void QCPLayoutElement::parentPlotInitialized(QCustomPlot *parentPlot) +{ + foreach (QCPLayoutElement* el, elements(false)) + { + if (!el->parentPlot()) + el->initializeParentPlot(parentPlot); + } +} + +/*! \internal + + Returns the margin size for this \a side. It is used if automatic margins is enabled for this \a + side (see \ref setAutoMargins). If a minimum margin was set with \ref setMinimumMargins, the + returned value will not be smaller than the specified minimum margin. + + The default implementation just returns the respective manual margin (\ref setMargins) or the + minimum margin, whichever is larger. +*/ +int QCPLayoutElement::calculateAutoMargin(QCP::MarginSide side) +{ + return qMax(QCP::getMarginValue(mMargins, side), QCP::getMarginValue(mMinimumMargins, side)); +} + +//////////////////////////////////////////////////////////////////////////////////////////////////// +//////////////////// QCPLayout +//////////////////////////////////////////////////////////////////////////////////////////////////// + +/*! \class QCPLayout + \brief The abstract base class for layouts + + This is an abstract base class for layout elements whose main purpose is to define the position + and size of other child layout elements. In most cases, layouts don't draw anything themselves + (but there are exceptions to this, e.g. QCPLegend). + + QCPLayout derives from QCPLayoutElement, and thus can itself be nested in other layouts. + + QCPLayout introduces a common interface for accessing and manipulating the child elements. Those + functions are most notably \ref elementCount, \ref elementAt, \ref takeAt, \ref take, \ref + simplify, \ref removeAt, \ref remove and \ref clear. Individual subclasses may add more functions + to this interface which are more specialized to the form of the layout. For example, \ref + QCPLayoutGrid adds functions that take row and column indices to access cells of the layout grid + more conveniently. + + Since this is an abstract base class, you can't instantiate it directly. Rather use one of its + subclasses like QCPLayoutGrid or QCPLayoutInset. + + For a general introduction to the layout system, see the dedicated documentation page \ref + thelayoutsystem "The Layout System". +*/ + +/* start documentation of pure virtual functions */ + +/*! \fn virtual int QCPLayout::elementCount() const = 0 + + Returns the number of elements/cells in the layout. + + \see elements, elementAt +*/ + +/*! \fn virtual QCPLayoutElement* QCPLayout::elementAt(int index) const = 0 + + Returns the element in the cell with the given \a index. If \a index is invalid, returns 0. + + Note that even if \a index is valid, the respective cell may be empty in some layouts (e.g. + QCPLayoutGrid), so this function may return 0 in those cases. You may use this function to check + whether a cell is empty or not. + + \see elements, elementCount, takeAt +*/ + +/*! \fn virtual QCPLayoutElement* QCPLayout::takeAt(int index) = 0 + + Removes the element with the given \a index from the layout and returns it. + + If the \a index is invalid or the cell with that index is empty, returns 0. + + Note that some layouts don't remove the respective cell right away but leave an empty cell after + successful removal of the layout element. To collapse empty cells, use \ref simplify. + + \see elementAt, take +*/ + +/*! \fn virtual bool QCPLayout::take(QCPLayoutElement* element) = 0 + + Removes the specified \a element from the layout and returns true on success. + + If the \a element isn't in this layout, returns false. + + Note that some layouts don't remove the respective cell right away but leave an empty cell after + successful removal of the layout element. To collapse empty cells, use \ref simplify. + + \see takeAt +*/ + +/* end documentation of pure virtual functions */ + +/*! + Creates an instance of QCPLayout and sets default values. Note that since QCPLayout + is an abstract base class, it can't be instantiated directly. +*/ +QCPLayout::QCPLayout() +{ +} + +/*! + First calls the QCPLayoutElement::update base class implementation to update the margins on this + layout. + + Then calls \ref updateLayout which subclasses reimplement to reposition and resize their cells. + + Finally, \ref update is called on all child elements. +*/ +void QCPLayout::update(UpdatePhase phase) +{ + QCPLayoutElement::update(phase); + + // set child element rects according to layout: + if (phase == upLayout) + updateLayout(); + + // propagate update call to child elements: + const int elCount = elementCount(); + for (int i=0; iupdate(phase); + } +} + +/* inherits documentation from base class */ +QList QCPLayout::elements(bool recursive) const +{ + const int c = elementCount(); + QList result; +#if QT_VERSION >= QT_VERSION_CHECK(4, 7, 0) + result.reserve(c); +#endif + for (int i=0; ielements(recursive); + } + } + return result; +} + +/*! + Simplifies the layout by collapsing empty cells. The exact behavior depends on subclasses, the + default implementation does nothing. + + Not all layouts need simplification. For example, QCPLayoutInset doesn't use explicit + simplification while QCPLayoutGrid does. +*/ +void QCPLayout::simplify() +{ +} + +/*! + Removes and deletes the element at the provided \a index. Returns true on success. If \a index is + invalid or points to an empty cell, returns false. + + This function internally uses \ref takeAt to remove the element from the layout and then deletes + the returned element. Note that some layouts don't remove the respective cell right away but leave an + empty cell after successful removal of the layout element. To collapse empty cells, use \ref + simplify. + + \see remove, takeAt +*/ +bool QCPLayout::removeAt(int index) +{ + if (QCPLayoutElement *el = takeAt(index)) + { + delete el; + return true; + } else + return false; +} + +/*! + Removes and deletes the provided \a element. Returns true on success. If \a element is not in the + layout, returns false. + + This function internally uses \ref takeAt to remove the element from the layout and then deletes + the element. Note that some layouts don't remove the respective cell right away but leave an + empty cell after successful removal of the layout element. To collapse empty cells, use \ref + simplify. + + \see removeAt, take +*/ +bool QCPLayout::remove(QCPLayoutElement *element) +{ + if (take(element)) + { + delete element; + return true; + } else + return false; +} + +/*! + Removes and deletes all layout elements in this layout. Finally calls \ref simplify to make sure + all empty cells are collapsed. + + \see remove, removeAt +*/ +void QCPLayout::clear() +{ + for (int i=elementCount()-1; i>=0; --i) + { + if (elementAt(i)) + removeAt(i); + } + simplify(); +} + +/*! + Subclasses call this method to report changed (minimum/maximum) size constraints. + + If the parent of this layout is again a QCPLayout, forwards the call to the parent's \ref + sizeConstraintsChanged. If the parent is a QWidget (i.e. is the \ref QCustomPlot::plotLayout of + QCustomPlot), calls QWidget::updateGeometry, so if the QCustomPlot widget is inside a Qt QLayout, + it may update itself and resize cells accordingly. +*/ +void QCPLayout::sizeConstraintsChanged() const +{ + if (QWidget *w = qobject_cast(parent())) + w->updateGeometry(); + else if (QCPLayout *l = qobject_cast(parent())) + l->sizeConstraintsChanged(); +} + +/*! \internal + + Subclasses reimplement this method to update the position and sizes of the child elements/cells + via calling their \ref QCPLayoutElement::setOuterRect. The default implementation does nothing. + + The geometry used as a reference is the inner \ref rect of this layout. Child elements should stay + within that rect. + + \ref getSectionSizes may help with the reimplementation of this function. + + \see update +*/ +void QCPLayout::updateLayout() +{ +} + + +/*! \internal + + Associates \a el with this layout. This is done by setting the \ref QCPLayoutElement::layout, the + \ref QCPLayerable::parentLayerable and the QObject parent to this layout. + + Further, if \a el didn't previously have a parent plot, calls \ref + QCPLayerable::initializeParentPlot on \a el to set the paret plot. + + This method is used by subclass specific methods that add elements to the layout. Note that this + method only changes properties in \a el. The removal from the old layout and the insertion into + the new layout must be done additionally. +*/ +void QCPLayout::adoptElement(QCPLayoutElement *el) +{ + if (el) + { + el->mParentLayout = this; + el->setParentLayerable(this); + el->setParent(this); + if (!el->parentPlot()) + el->initializeParentPlot(mParentPlot); + } else + qDebug() << Q_FUNC_INFO << "Null element passed"; +} + +/*! \internal + + Disassociates \a el from this layout. This is done by setting the \ref QCPLayoutElement::layout + and the \ref QCPLayerable::parentLayerable to zero. The QObject parent is set to the parent + QCustomPlot. + + This method is used by subclass specific methods that remove elements from the layout (e.g. \ref + take or \ref takeAt). Note that this method only changes properties in \a el. The removal from + the old layout must be done additionally. +*/ +void QCPLayout::releaseElement(QCPLayoutElement *el) +{ + if (el) + { + el->mParentLayout = 0; + el->setParentLayerable(0); + el->setParent(mParentPlot); + // Note: Don't initializeParentPlot(0) here, because layout element will stay in same parent plot + } else + qDebug() << Q_FUNC_INFO << "Null element passed"; +} + +/*! \internal + + This is a helper function for the implementation of \ref updateLayout in subclasses. + + It calculates the sizes of one-dimensional sections with provided constraints on maximum section + sizes, minimum section sizes, relative stretch factors and the final total size of all sections. + + The QVector entries refer to the sections. Thus all QVectors must have the same size. + + \a maxSizes gives the maximum allowed size of each section. If there shall be no maximum size + imposed, set all vector values to Qt's QWIDGETSIZE_MAX. + + \a minSizes gives the minimum allowed size of each section. If there shall be no minimum size + imposed, set all vector values to zero. If the \a minSizes entries add up to a value greater than + \a totalSize, sections will be scaled smaller than the proposed minimum sizes. (In other words, + not exceeding the allowed total size is taken to be more important than not going below minimum + section sizes.) + + \a stretchFactors give the relative proportions of the sections to each other. If all sections + shall be scaled equally, set all values equal. If the first section shall be double the size of + each individual other section, set the first number of \a stretchFactors to double the value of + the other individual values (e.g. {2, 1, 1, 1}). + + \a totalSize is the value that the final section sizes will add up to. Due to rounding, the + actual sum may differ slightly. If you want the section sizes to sum up to exactly that value, + you could distribute the remaining difference on the sections. + + The return value is a QVector containing the section sizes. +*/ +QVector QCPLayout::getSectionSizes(QVector maxSizes, QVector minSizes, QVector stretchFactors, int totalSize) const +{ + if (maxSizes.size() != minSizes.size() || minSizes.size() != stretchFactors.size()) + { + qDebug() << Q_FUNC_INFO << "Passed vector sizes aren't equal:" << maxSizes << minSizes << stretchFactors; + return QVector(); + } + if (stretchFactors.isEmpty()) + return QVector(); + int sectionCount = stretchFactors.size(); + QVector sectionSizes(sectionCount); + // if provided total size is forced smaller than total minimum size, ignore minimum sizes (squeeze sections): + int minSizeSum = 0; + for (int i=0; i minimumLockedSections; + QList unfinishedSections; + for (int i=0; i result(sectionCount); + for (int i=0; i= 0 && row < mElements.size()) + { + if (column >= 0 && column < mElements.first().size()) + { + if (QCPLayoutElement *result = mElements.at(row).at(column)) + return result; + else + qDebug() << Q_FUNC_INFO << "Requested cell is empty. Row:" << row << "Column:" << column; + } else + qDebug() << Q_FUNC_INFO << "Invalid column. Row:" << row << "Column:" << column; + } else + qDebug() << Q_FUNC_INFO << "Invalid row. Row:" << row << "Column:" << column; + return 0; +} + +/*! + Returns the number of rows in the layout. + + \see columnCount +*/ +int QCPLayoutGrid::rowCount() const +{ + return mElements.size(); +} + +/*! + Returns the number of columns in the layout. + + \see rowCount +*/ +int QCPLayoutGrid::columnCount() const +{ + if (mElements.size() > 0) + return mElements.first().size(); + else + return 0; +} + +/*! + Adds the \a element to cell with \a row and \a column. If \a element is already in a layout, it + is first removed from there. If \a row or \a column don't exist yet, the layout is expanded + accordingly. + + Returns true if the element was added successfully, i.e. if the cell at \a row and \a column + didn't already have an element. + + \see element, hasElement, take, remove +*/ +bool QCPLayoutGrid::addElement(int row, int column, QCPLayoutElement *element) +{ + if (element) + { + if (!hasElement(row, column)) + { + if (element->layout()) // remove from old layout first + element->layout()->take(element); + expandTo(row+1, column+1); + mElements[row][column] = element; + adoptElement(element); + return true; + } else + qDebug() << Q_FUNC_INFO << "There is already an element in the specified row/column:" << row << column; + } else + qDebug() << Q_FUNC_INFO << "Can't add null element to row/column:" << row << column; + return false; +} + +/*! + Returns whether the cell at \a row and \a column exists and contains a valid element, i.e. isn't + empty. + + \see element +*/ +bool QCPLayoutGrid::hasElement(int row, int column) +{ + if (row >= 0 && row < rowCount() && column >= 0 && column < columnCount()) + return mElements.at(row).at(column); + else + return false; +} + +/*! + Sets the stretch \a factor of \a column. + + Stretch factors control the relative sizes of rows and columns. Cells will not be resized beyond + their minimum and maximum widths/heights (\ref QCPLayoutElement::setMinimumSize, \ref + QCPLayoutElement::setMaximumSize), regardless of the stretch factor. + + The default stretch factor of newly created rows/columns is 1. + + \see setColumnStretchFactors, setRowStretchFactor +*/ +void QCPLayoutGrid::setColumnStretchFactor(int column, double factor) +{ + if (column >= 0 && column < columnCount()) + { + if (factor > 0) + mColumnStretchFactors[column] = factor; + else + qDebug() << Q_FUNC_INFO << "Invalid stretch factor, must be positive:" << factor; + } else + qDebug() << Q_FUNC_INFO << "Invalid column:" << column; +} + +/*! + Sets the stretch \a factors of all columns. \a factors must have the size \ref columnCount. + + Stretch factors control the relative sizes of rows and columns. Cells will not be resized beyond + their minimum and maximum widths/heights (\ref QCPLayoutElement::setMinimumSize, \ref + QCPLayoutElement::setMaximumSize), regardless of the stretch factor. + + The default stretch factor of newly created rows/columns is 1. + + \see setColumnStretchFactor, setRowStretchFactors +*/ +void QCPLayoutGrid::setColumnStretchFactors(const QList &factors) +{ + if (factors.size() == mColumnStretchFactors.size()) + { + mColumnStretchFactors = factors; + for (int i=0; i= 0 && row < rowCount()) + { + if (factor > 0) + mRowStretchFactors[row] = factor; + else + qDebug() << Q_FUNC_INFO << "Invalid stretch factor, must be positive:" << factor; + } else + qDebug() << Q_FUNC_INFO << "Invalid row:" << row; +} + +/*! + Sets the stretch \a factors of all rows. \a factors must have the size \ref rowCount. + + Stretch factors control the relative sizes of rows and columns. Cells will not be resized beyond + their minimum and maximum widths/heights (\ref QCPLayoutElement::setMinimumSize, \ref + QCPLayoutElement::setMaximumSize), regardless of the stretch factor. + + The default stretch factor of newly created rows/columns is 1. + + \see setRowStretchFactor, setColumnStretchFactors +*/ +void QCPLayoutGrid::setRowStretchFactors(const QList &factors) +{ + if (factors.size() == mRowStretchFactors.size()) + { + mRowStretchFactors = factors; + for (int i=0; i()); + mRowStretchFactors.append(1); + } + // go through rows and expand columns as necessary: + int newColCount = qMax(columnCount(), newColumnCount); + for (int i=0; i rowCount()) + newIndex = rowCount(); + + mRowStretchFactors.insert(newIndex, 1); + QList newRow; + for (int col=0; col columnCount()) + newIndex = columnCount(); + + mColumnStretchFactors.insert(newIndex, 1); + for (int row=0; row minColWidths, minRowHeights, maxColWidths, maxRowHeights; + getMinimumRowColSizes(&minColWidths, &minRowHeights); + getMaximumRowColSizes(&maxColWidths, &maxRowHeights); + + int totalRowSpacing = (rowCount()-1) * mRowSpacing; + int totalColSpacing = (columnCount()-1) * mColumnSpacing; + QVector colWidths = getSectionSizes(maxColWidths, minColWidths, mColumnStretchFactors.toVector(), mRect.width()-totalColSpacing); + QVector rowHeights = getSectionSizes(maxRowHeights, minRowHeights, mRowStretchFactors.toVector(), mRect.height()-totalRowSpacing); + + // go through cells and set rects accordingly: + int yOffset = mRect.top(); + for (int row=0; row 0) + yOffset += rowHeights.at(row-1)+mRowSpacing; + int xOffset = mRect.left(); + for (int col=0; col 0) + xOffset += colWidths.at(col-1)+mColumnSpacing; + if (mElements.at(row).at(col)) + mElements.at(row).at(col)->setOuterRect(QRect(xOffset, yOffset, colWidths.at(col), rowHeights.at(row))); + } + } +} + +/* inherits documentation from base class */ +int QCPLayoutGrid::elementCount() const +{ + return rowCount()*columnCount(); +} + +/* inherits documentation from base class */ +QCPLayoutElement *QCPLayoutGrid::elementAt(int index) const +{ + if (index >= 0 && index < elementCount()) + return mElements.at(index / columnCount()).at(index % columnCount()); + else + return 0; +} + +/* inherits documentation from base class */ +QCPLayoutElement *QCPLayoutGrid::takeAt(int index) +{ + if (QCPLayoutElement *el = elementAt(index)) + { + releaseElement(el); + mElements[index / columnCount()][index % columnCount()] = 0; + return el; + } else + { + qDebug() << Q_FUNC_INFO << "Attempt to take invalid index:" << index; + return 0; + } +} + +/* inherits documentation from base class */ +bool QCPLayoutGrid::take(QCPLayoutElement *element) +{ + if (element) + { + for (int i=0; i QCPLayoutGrid::elements(bool recursive) const +{ + QList result; + int colC = columnCount(); + int rowC = rowCount(); +#if QT_VERSION >= QT_VERSION_CHECK(4, 7, 0) + result.reserve(colC*rowC); +#endif + for (int row=0; rowelements(recursive); + } + } + return result; +} + +/*! + Simplifies the layout by collapsing rows and columns which only contain empty cells. +*/ +void QCPLayoutGrid::simplify() +{ + // remove rows with only empty cells: + for (int row=rowCount()-1; row>=0; --row) + { + bool hasElements = false; + for (int col=0; col=0; --col) + { + bool hasElements = false; + for (int row=0; row minColWidths, minRowHeights; + getMinimumRowColSizes(&minColWidths, &minRowHeights); + QSize result(0, 0); + for (int i=0; i maxColWidths, maxRowHeights; + getMaximumRowColSizes(&maxColWidths, &maxRowHeights); + + QSize result(0, 0); + for (int i=0; i *minColWidths, QVector *minRowHeights) const +{ + *minColWidths = QVector(columnCount(), 0); + *minRowHeights = QVector(rowCount(), 0); + for (int row=0; rowminimumSizeHint(); + QSize min = mElements.at(row).at(col)->minimumSize(); + QSize final(min.width() > 0 ? min.width() : minHint.width(), min.height() > 0 ? min.height() : minHint.height()); + if (minColWidths->at(col) < final.width()) + (*minColWidths)[col] = final.width(); + if (minRowHeights->at(row) < final.height()) + (*minRowHeights)[row] = final.height(); + } + } + } +} + +/*! \internal + + Places the maximum column widths and row heights into \a maxColWidths and \a maxRowHeights + respectively. + + The maximum height of a row is the smallest maximum height of any element in that row. The + maximum width of a column is the smallest maximum width of any element in that column. + + This is a helper function for \ref updateLayout. + + \see getMinimumRowColSizes +*/ +void QCPLayoutGrid::getMaximumRowColSizes(QVector *maxColWidths, QVector *maxRowHeights) const +{ + *maxColWidths = QVector(columnCount(), QWIDGETSIZE_MAX); + *maxRowHeights = QVector(rowCount(), QWIDGETSIZE_MAX); + for (int row=0; rowmaximumSizeHint(); + QSize max = mElements.at(row).at(col)->maximumSize(); + QSize final(max.width() < QWIDGETSIZE_MAX ? max.width() : maxHint.width(), max.height() < QWIDGETSIZE_MAX ? max.height() : maxHint.height()); + if (maxColWidths->at(col) > final.width()) + (*maxColWidths)[col] = final.width(); + if (maxRowHeights->at(row) > final.height()) + (*maxRowHeights)[row] = final.height(); + } + } + } +} + + +//////////////////////////////////////////////////////////////////////////////////////////////////// +//////////////////// QCPLayoutInset +//////////////////////////////////////////////////////////////////////////////////////////////////// +/*! \class QCPLayoutInset + \brief A layout that places child elements aligned to the border or arbitrarily positioned + + Elements are placed either aligned to the border or at arbitrary position in the area of the + layout. Which placement applies is controlled with the \ref InsetPlacement (\ref + setInsetPlacement). + + Elements are added via \ref addElement(QCPLayoutElement *element, Qt::Alignment alignment) or + addElement(QCPLayoutElement *element, const QRectF &rect). If the first method is used, the inset + placement will default to \ref ipBorderAligned and the element will be aligned according to the + \a alignment parameter. The second method defaults to \ref ipFree and allows placing elements at + arbitrary position and size, defined by \a rect. + + The alignment or rect can be set via \ref setInsetAlignment or \ref setInsetRect, respectively. + + This is the layout that every QCPAxisRect has as \ref QCPAxisRect::insetLayout. +*/ + +/* start documentation of inline functions */ + +/*! \fn virtual void QCPLayoutInset::simplify() + + The QCPInsetLayout does not need simplification since it can never have empty cells due to its + linear index structure. This method does nothing. +*/ + +/* end documentation of inline functions */ + +/*! + Creates an instance of QCPLayoutInset and sets default values. +*/ +QCPLayoutInset::QCPLayoutInset() +{ +} + +QCPLayoutInset::~QCPLayoutInset() +{ + // clear all child layout elements. This is important because only the specific layouts know how + // to handle removing elements (clear calls virtual removeAt method to do that). + clear(); +} + +/*! + Returns the placement type of the element with the specified \a index. +*/ +QCPLayoutInset::InsetPlacement QCPLayoutInset::insetPlacement(int index) const +{ + if (elementAt(index)) + return mInsetPlacement.at(index); + else + { + qDebug() << Q_FUNC_INFO << "Invalid element index:" << index; + return ipFree; + } +} + +/*! + Returns the alignment of the element with the specified \a index. The alignment only has a + meaning, if the inset placement (\ref setInsetPlacement) is \ref ipBorderAligned. +*/ +Qt::Alignment QCPLayoutInset::insetAlignment(int index) const +{ + if (elementAt(index)) + return mInsetAlignment.at(index); + else + { + qDebug() << Q_FUNC_INFO << "Invalid element index:" << index; + return 0; + } +} + +/*! + Returns the rect of the element with the specified \a index. The rect only has a + meaning, if the inset placement (\ref setInsetPlacement) is \ref ipFree. +*/ +QRectF QCPLayoutInset::insetRect(int index) const +{ + if (elementAt(index)) + return mInsetRect.at(index); + else + { + qDebug() << Q_FUNC_INFO << "Invalid element index:" << index; + return QRectF(); + } +} + +/*! + Sets the inset placement type of the element with the specified \a index to \a placement. + + \see InsetPlacement +*/ +void QCPLayoutInset::setInsetPlacement(int index, QCPLayoutInset::InsetPlacement placement) +{ + if (elementAt(index)) + mInsetPlacement[index] = placement; + else + qDebug() << Q_FUNC_INFO << "Invalid element index:" << index; +} + +/*! + If the inset placement (\ref setInsetPlacement) is \ref ipBorderAligned, this function + is used to set the alignment of the element with the specified \a index to \a alignment. + + \a alignment is an or combination of the following alignment flags: Qt::AlignLeft, + Qt::AlignHCenter, Qt::AlighRight, Qt::AlignTop, Qt::AlignVCenter, Qt::AlignBottom. Any other + alignment flags will be ignored. +*/ +void QCPLayoutInset::setInsetAlignment(int index, Qt::Alignment alignment) +{ + if (elementAt(index)) + mInsetAlignment[index] = alignment; + else + qDebug() << Q_FUNC_INFO << "Invalid element index:" << index; +} + +/*! + If the inset placement (\ref setInsetPlacement) is \ref ipFree, this function is used to set the + position and size of the element with the specified \a index to \a rect. + + \a rect is given in fractions of the whole inset layout rect. So an inset with rect (0, 0, 1, 1) + will span the entire layout. An inset with rect (0.6, 0.1, 0.35, 0.35) will be in the top right + corner of the layout, with 35% width and height of the parent layout. + + Note that the minimum and maximum sizes of the embedded element (\ref + QCPLayoutElement::setMinimumSize, \ref QCPLayoutElement::setMaximumSize) are enforced. +*/ +void QCPLayoutInset::setInsetRect(int index, const QRectF &rect) +{ + if (elementAt(index)) + mInsetRect[index] = rect; + else + qDebug() << Q_FUNC_INFO << "Invalid element index:" << index; +} + +/* inherits documentation from base class */ +void QCPLayoutInset::updateLayout() +{ + for (int i=0; iminimumSizeHint(); + QSize maxSizeHint = mElements.at(i)->maximumSizeHint(); + finalMinSize.setWidth(mElements.at(i)->minimumSize().width() > 0 ? mElements.at(i)->minimumSize().width() : minSizeHint.width()); + finalMinSize.setHeight(mElements.at(i)->minimumSize().height() > 0 ? mElements.at(i)->minimumSize().height() : minSizeHint.height()); + finalMaxSize.setWidth(mElements.at(i)->maximumSize().width() < QWIDGETSIZE_MAX ? mElements.at(i)->maximumSize().width() : maxSizeHint.width()); + finalMaxSize.setHeight(mElements.at(i)->maximumSize().height() < QWIDGETSIZE_MAX ? mElements.at(i)->maximumSize().height() : maxSizeHint.height()); + if (mInsetPlacement.at(i) == ipFree) + { + insetRect = QRect(rect().x()+rect().width()*mInsetRect.at(i).x(), + rect().y()+rect().height()*mInsetRect.at(i).y(), + rect().width()*mInsetRect.at(i).width(), + rect().height()*mInsetRect.at(i).height()); + if (insetRect.size().width() < finalMinSize.width()) + insetRect.setWidth(finalMinSize.width()); + if (insetRect.size().height() < finalMinSize.height()) + insetRect.setHeight(finalMinSize.height()); + if (insetRect.size().width() > finalMaxSize.width()) + insetRect.setWidth(finalMaxSize.width()); + if (insetRect.size().height() > finalMaxSize.height()) + insetRect.setHeight(finalMaxSize.height()); + } else if (mInsetPlacement.at(i) == ipBorderAligned) + { + insetRect.setSize(finalMinSize); + Qt::Alignment al = mInsetAlignment.at(i); + if (al.testFlag(Qt::AlignLeft)) insetRect.moveLeft(rect().x()); + else if (al.testFlag(Qt::AlignRight)) insetRect.moveRight(rect().x()+rect().width()); + else insetRect.moveLeft(rect().x()+rect().width()*0.5-finalMinSize.width()*0.5); // default to Qt::AlignHCenter + if (al.testFlag(Qt::AlignTop)) insetRect.moveTop(rect().y()); + else if (al.testFlag(Qt::AlignBottom)) insetRect.moveBottom(rect().y()+rect().height()); + else insetRect.moveTop(rect().y()+rect().height()*0.5-finalMinSize.height()*0.5); // default to Qt::AlignVCenter + } + mElements.at(i)->setOuterRect(insetRect); + } +} + +/* inherits documentation from base class */ +int QCPLayoutInset::elementCount() const +{ + return mElements.size(); +} + +/* inherits documentation from base class */ +QCPLayoutElement *QCPLayoutInset::elementAt(int index) const +{ + if (index >= 0 && index < mElements.size()) + return mElements.at(index); + else + return 0; +} + +/* inherits documentation from base class */ +QCPLayoutElement *QCPLayoutInset::takeAt(int index) +{ + if (QCPLayoutElement *el = elementAt(index)) + { + releaseElement(el); + mElements.removeAt(index); + mInsetPlacement.removeAt(index); + mInsetAlignment.removeAt(index); + mInsetRect.removeAt(index); + return el; + } else + { + qDebug() << Q_FUNC_INFO << "Attempt to take invalid index:" << index; + return 0; + } +} + +/* inherits documentation from base class */ +bool QCPLayoutInset::take(QCPLayoutElement *element) +{ + if (element) + { + for (int i=0; irealVisibility() && mElements.at(i)->selectTest(pos, onlySelectable) >= 0) + return mParentPlot->selectionTolerance()*0.99; + } + return -1; +} + +/*! + Adds the specified \a element to the layout as an inset aligned at the border (\ref + setInsetAlignment is initialized with \ref ipBorderAligned). The alignment is set to \a + alignment. + + \a alignment is an or combination of the following alignment flags: Qt::AlignLeft, + Qt::AlignHCenter, Qt::AlighRight, Qt::AlignTop, Qt::AlignVCenter, Qt::AlignBottom. Any other + alignment flags will be ignored. + + \see addElement(QCPLayoutElement *element, const QRectF &rect) +*/ +void QCPLayoutInset::addElement(QCPLayoutElement *element, Qt::Alignment alignment) +{ + if (element) + { + if (element->layout()) // remove from old layout first + element->layout()->take(element); + mElements.append(element); + mInsetPlacement.append(ipBorderAligned); + mInsetAlignment.append(alignment); + mInsetRect.append(QRectF(0.6, 0.6, 0.4, 0.4)); + adoptElement(element); + } else + qDebug() << Q_FUNC_INFO << "Can't add null element"; +} + +/*! + Adds the specified \a element to the layout as an inset with free positioning/sizing (\ref + setInsetAlignment is initialized with \ref ipFree). The position and size is set to \a + rect. + + \a rect is given in fractions of the whole inset layout rect. So an inset with rect (0, 0, 1, 1) + will span the entire layout. An inset with rect (0.6, 0.1, 0.35, 0.35) will be in the top right + corner of the layout, with 35% width and height of the parent layout. + + \see addElement(QCPLayoutElement *element, Qt::Alignment alignment) +*/ +void QCPLayoutInset::addElement(QCPLayoutElement *element, const QRectF &rect) +{ + if (element) + { + if (element->layout()) // remove from old layout first + element->layout()->take(element); + mElements.append(element); + mInsetPlacement.append(ipFree); + mInsetAlignment.append(Qt::AlignRight|Qt::AlignTop); + mInsetRect.append(rect); + adoptElement(element); + } else + qDebug() << Q_FUNC_INFO << "Can't add null element"; +} + + +//////////////////////////////////////////////////////////////////////////////////////////////////// +//////////////////// QCPLineEnding +//////////////////////////////////////////////////////////////////////////////////////////////////// + +/*! \class QCPLineEnding + \brief Handles the different ending decorations for line-like items + + \image html QCPLineEnding.png "The various ending styles currently supported" + + For every ending a line-like item has, an instance of this class exists. For example, QCPItemLine + has two endings which can be set with QCPItemLine::setHead and QCPItemLine::setTail. + + The styles themselves are defined via the enum QCPLineEnding::EndingStyle. Most decorations can + be modified regarding width and length, see \ref setWidth and \ref setLength. The direction of + the ending decoration (e.g. direction an arrow is pointing) is controlled by the line-like item. + For example, when both endings of a QCPItemLine are set to be arrows, they will point to opposite + directions, e.g. "outward". This can be changed by \ref setInverted, which would make the + respective arrow point inward. + + Note that due to the overloaded QCPLineEnding constructor, you may directly specify a + QCPLineEnding::EndingStyle where actually a QCPLineEnding is expected, e.g. + \snippet documentation/doc-code-snippets/mainwindow.cpp qcplineending-sethead +*/ + +/*! + Creates a QCPLineEnding instance with default values (style \ref esNone). +*/ +QCPLineEnding::QCPLineEnding() : + mStyle(esNone), + mWidth(8), + mLength(10), + mInverted(false) +{ +} + +/*! + Creates a QCPLineEnding instance with the specified values. +*/ +QCPLineEnding::QCPLineEnding(QCPLineEnding::EndingStyle style, double width, double length, bool inverted) : + mStyle(style), + mWidth(width), + mLength(length), + mInverted(inverted) +{ +} + +/*! + Sets the style of the ending decoration. +*/ +void QCPLineEnding::setStyle(QCPLineEnding::EndingStyle style) +{ + mStyle = style; +} + +/*! + Sets the width of the ending decoration, if the style supports it. On arrows, for example, the + width defines the size perpendicular to the arrow's pointing direction. + + \see setLength +*/ +void QCPLineEnding::setWidth(double width) +{ + mWidth = width; +} + +/*! + Sets the length of the ending decoration, if the style supports it. On arrows, for example, the + length defines the size in pointing direction. + + \see setWidth +*/ +void QCPLineEnding::setLength(double length) +{ + mLength = length; +} + +/*! + Sets whether the ending decoration shall be inverted. For example, an arrow decoration will point + inward when \a inverted is set to true. + + Note that also the \a width direction is inverted. For symmetrical ending styles like arrows or + discs, this doesn't make a difference. However, asymmetric styles like \ref esHalfBar are + affected by it, which can be used to control to which side the half bar points to. +*/ +void QCPLineEnding::setInverted(bool inverted) +{ + mInverted = inverted; +} + +/*! \internal + + Returns the maximum pixel radius the ending decoration might cover, starting from the position + the decoration is drawn at (typically a line ending/\ref QCPItemPosition of an item). + + This is relevant for clipping. Only omit painting of the decoration when the position where the + decoration is supposed to be drawn is farther away from the clipping rect than the returned + distance. +*/ +double QCPLineEnding::boundingDistance() const +{ + switch (mStyle) + { + case esNone: + return 0; + + case esFlatArrow: + case esSpikeArrow: + case esLineArrow: + case esSkewedBar: + return qSqrt(mWidth*mWidth+mLength*mLength); // items that have width and length + + case esDisc: + case esSquare: + case esDiamond: + case esBar: + case esHalfBar: + return mWidth*1.42; // items that only have a width -> width*sqrt(2) + + } + return 0; +} + +/*! + Starting from the origin of this line ending (which is style specific), returns the length + covered by the line ending symbol, in backward direction. + + For example, the \ref esSpikeArrow has a shorter real length than a \ref esFlatArrow, even if + both have the same \ref setLength value, because the spike arrow has an inward curved back, which + reduces the length along its center axis (the drawing origin for arrows is at the tip). + + This function is used for precise, style specific placement of line endings, for example in + QCPAxes. +*/ +double QCPLineEnding::realLength() const +{ + switch (mStyle) + { + case esNone: + case esLineArrow: + case esSkewedBar: + case esBar: + case esHalfBar: + return 0; + + case esFlatArrow: + return mLength; + + case esDisc: + case esSquare: + case esDiamond: + return mWidth*0.5; + + case esSpikeArrow: + return mLength*0.8; + } + return 0; +} + +/*! \internal + + Draws the line ending with the specified \a painter at the position \a pos. The direction of the + line ending is controlled with \a dir. +*/ +void QCPLineEnding::draw(QCPPainter *painter, const QVector2D &pos, const QVector2D &dir) const +{ + if (mStyle == esNone) + return; + + QVector2D lengthVec(dir.normalized()); + if (lengthVec.isNull()) + lengthVec = QVector2D(1, 0); + QVector2D widthVec(-lengthVec.y(), lengthVec.x()); + lengthVec *= (float)(mLength*(mInverted ? -1 : 1)); + widthVec *= (float)(mWidth*0.5*(mInverted ? -1 : 1)); + + QPen penBackup = painter->pen(); + QBrush brushBackup = painter->brush(); + QPen miterPen = penBackup; + miterPen.setJoinStyle(Qt::MiterJoin); // to make arrow heads spikey + QBrush brush(painter->pen().color(), Qt::SolidPattern); + switch (mStyle) + { + case esNone: break; + case esFlatArrow: + { + QPointF points[3] = {pos.toPointF(), + (pos-lengthVec+widthVec).toPointF(), + (pos-lengthVec-widthVec).toPointF() + }; + painter->setPen(miterPen); + painter->setBrush(brush); + painter->drawConvexPolygon(points, 3); + painter->setBrush(brushBackup); + painter->setPen(penBackup); + break; + } + case esSpikeArrow: + { + QPointF points[4] = {pos.toPointF(), + (pos-lengthVec+widthVec).toPointF(), + (pos-lengthVec*0.8f).toPointF(), + (pos-lengthVec-widthVec).toPointF() + }; + painter->setPen(miterPen); + painter->setBrush(brush); + painter->drawConvexPolygon(points, 4); + painter->setBrush(brushBackup); + painter->setPen(penBackup); + break; + } + case esLineArrow: + { + QPointF points[3] = {(pos-lengthVec+widthVec).toPointF(), + pos.toPointF(), + (pos-lengthVec-widthVec).toPointF() + }; + painter->setPen(miterPen); + painter->drawPolyline(points, 3); + painter->setPen(penBackup); + break; + } + case esDisc: + { + painter->setBrush(brush); + painter->drawEllipse(pos.toPointF(), mWidth*0.5, mWidth*0.5); + painter->setBrush(brushBackup); + break; + } + case esSquare: + { + QVector2D widthVecPerp(-widthVec.y(), widthVec.x()); + QPointF points[4] = {(pos-widthVecPerp+widthVec).toPointF(), + (pos-widthVecPerp-widthVec).toPointF(), + (pos+widthVecPerp-widthVec).toPointF(), + (pos+widthVecPerp+widthVec).toPointF() + }; + painter->setPen(miterPen); + painter->setBrush(brush); + painter->drawConvexPolygon(points, 4); + painter->setBrush(brushBackup); + painter->setPen(penBackup); + break; + } + case esDiamond: + { + QVector2D widthVecPerp(-widthVec.y(), widthVec.x()); + QPointF points[4] = {(pos-widthVecPerp).toPointF(), + (pos-widthVec).toPointF(), + (pos+widthVecPerp).toPointF(), + (pos+widthVec).toPointF() + }; + painter->setPen(miterPen); + painter->setBrush(brush); + painter->drawConvexPolygon(points, 4); + painter->setBrush(brushBackup); + painter->setPen(penBackup); + break; + } + case esBar: + { + painter->drawLine((pos+widthVec).toPointF(), (pos-widthVec).toPointF()); + break; + } + case esHalfBar: + { + painter->drawLine((pos+widthVec).toPointF(), pos.toPointF()); + break; + } + case esSkewedBar: + { + if (qFuzzyIsNull(painter->pen().widthF()) && !painter->modes().testFlag(QCPPainter::pmNonCosmetic)) + { + // if drawing with cosmetic pen (perfectly thin stroke, happens only in vector exports), draw bar exactly on tip of line + painter->drawLine((pos+widthVec+lengthVec*0.2f*(mInverted?-1:1)).toPointF(), + (pos-widthVec-lengthVec*0.2f*(mInverted?-1:1)).toPointF()); + } else + { + // if drawing with thick (non-cosmetic) pen, shift bar a little in line direction to prevent line from sticking through bar slightly + painter->drawLine((pos+widthVec+lengthVec*0.2f*(mInverted?-1:1)+dir.normalized()*qMax(1.0f, (float)painter->pen().widthF())*0.5f).toPointF(), + (pos-widthVec-lengthVec*0.2f*(mInverted?-1:1)+dir.normalized()*qMax(1.0f, (float)painter->pen().widthF())*0.5f).toPointF()); + } + break; + } + } +} + +/*! \internal + \overload + + Draws the line ending. The direction is controlled with the \a angle parameter in radians. +*/ +void QCPLineEnding::draw(QCPPainter *painter, const QVector2D &pos, double angle) const +{ + draw(painter, pos, QVector2D(qCos(angle), qSin(angle))); +} + + +//////////////////////////////////////////////////////////////////////////////////////////////////// +//////////////////// QCPGrid +//////////////////////////////////////////////////////////////////////////////////////////////////// + +/*! \class QCPGrid + \brief Responsible for drawing the grid of a QCPAxis. + + This class is tightly bound to QCPAxis. Every axis owns a grid instance and uses it to draw the + grid lines, sub grid lines and zero-line. You can interact with the grid of an axis via \ref + QCPAxis::grid. Normally, you don't need to create an instance of QCPGrid yourself. + + The axis and grid drawing was split into two classes to allow them to be placed on different + layers (both QCPAxis and QCPGrid inherit from QCPLayerable). Thus it is possible to have the grid + in the background and the axes in the foreground, and any plottables/items in between. This + described situation is the default setup, see the QCPLayer documentation. +*/ + +/*! + Creates a QCPGrid instance and sets default values. + + You shouldn't instantiate grids on their own, since every QCPAxis brings its own QCPGrid. +*/ +QCPGrid::QCPGrid(QCPAxis *parentAxis) : + QCPLayerable(parentAxis->parentPlot(), QString(), parentAxis), + mParentAxis(parentAxis) +{ + // warning: this is called in QCPAxis constructor, so parentAxis members should not be accessed/called + setParent(parentAxis); + setPen(QPen(QColor(200,200,200), 0, Qt::DotLine)); + setSubGridPen(QPen(QColor(220,220,220), 0, Qt::DotLine)); + setZeroLinePen(QPen(QColor(200,200,200), 0, Qt::SolidLine)); + setSubGridVisible(false); + setAntialiased(false); + setAntialiasedSubGrid(false); + setAntialiasedZeroLine(false); +} + +/*! + Sets whether grid lines at sub tick marks are drawn. + + \see setSubGridPen +*/ +void QCPGrid::setSubGridVisible(bool visible) +{ + mSubGridVisible = visible; +} + +/*! + Sets whether sub grid lines are drawn antialiased. +*/ +void QCPGrid::setAntialiasedSubGrid(bool enabled) +{ + mAntialiasedSubGrid = enabled; +} + +/*! + Sets whether zero lines are drawn antialiased. +*/ +void QCPGrid::setAntialiasedZeroLine(bool enabled) +{ + mAntialiasedZeroLine = enabled; +} + +/*! + Sets the pen with which (major) grid lines are drawn. +*/ +void QCPGrid::setPen(const QPen &pen) +{ + mPen = pen; +} + +/*! + Sets the pen with which sub grid lines are drawn. +*/ +void QCPGrid::setSubGridPen(const QPen &pen) +{ + mSubGridPen = pen; +} + +/*! + Sets the pen with which zero lines are drawn. + + Zero lines are lines at value coordinate 0 which may be drawn with a different pen than other grid + lines. To disable zero lines and just draw normal grid lines at zero, set \a pen to Qt::NoPen. +*/ +void QCPGrid::setZeroLinePen(const QPen &pen) +{ + mZeroLinePen = pen; +} + +/*! \internal + + A convenience function to easily set the QPainter::Antialiased hint on the provided \a painter + before drawing the major grid lines. + + This is the antialiasing state the painter passed to the \ref draw method is in by default. + + This function takes into account the local setting of the antialiasing flag as well as the + overrides set with \ref QCustomPlot::setAntialiasedElements and \ref + QCustomPlot::setNotAntialiasedElements. + + \see setAntialiased +*/ +void QCPGrid::applyDefaultAntialiasingHint(QCPPainter *painter) const +{ + applyAntialiasingHint(painter, mAntialiased, QCP::aeGrid); +} + +/*! \internal + + Draws grid lines and sub grid lines at the positions of (sub) ticks of the parent axis, spanning + over the complete axis rect. Also draws the zero line, if appropriate (\ref setZeroLinePen). +*/ +void QCPGrid::draw(QCPPainter *painter) +{ + if (!mParentAxis) { qDebug() << Q_FUNC_INFO << "invalid parent axis"; return; } + + if (mSubGridVisible) + drawSubGridLines(painter); + drawGridLines(painter); +} + +/*! \internal + + Draws the main grid lines and possibly a zero line with the specified painter. + + This is a helper function called by \ref draw. +*/ +void QCPGrid::drawGridLines(QCPPainter *painter) const +{ + if (!mParentAxis) { qDebug() << Q_FUNC_INFO << "invalid parent axis"; return; } + + int lowTick = mParentAxis->mLowestVisibleTick; + int highTick = mParentAxis->mHighestVisibleTick; + double t; // helper variable, result of coordinate-to-pixel transforms + if (mParentAxis->orientation() == Qt::Horizontal) + { + // draw zeroline: + int zeroLineIndex = -1; + if (mZeroLinePen.style() != Qt::NoPen && mParentAxis->mRange.lower < 0 && mParentAxis->mRange.upper > 0) + { + applyAntialiasingHint(painter, mAntialiasedZeroLine, QCP::aeZeroLine); + painter->setPen(mZeroLinePen); + double epsilon = mParentAxis->range().size()*1E-6; // for comparing double to zero + for (int i=lowTick; i <= highTick; ++i) + { + if (qAbs(mParentAxis->mTickVector.at(i)) < epsilon) + { + zeroLineIndex = i; + t = mParentAxis->coordToPixel(mParentAxis->mTickVector.at(i)); // x + painter->drawLine(QLineF(t, mParentAxis->mAxisRect->bottom(), t, mParentAxis->mAxisRect->top())); + break; + } + } + } + // draw grid lines: + applyDefaultAntialiasingHint(painter); + painter->setPen(mPen); + for (int i=lowTick; i <= highTick; ++i) + { + if (i == zeroLineIndex) continue; // don't draw a gridline on top of the zeroline + t = mParentAxis->coordToPixel(mParentAxis->mTickVector.at(i)); // x + painter->drawLine(QLineF(t, mParentAxis->mAxisRect->bottom(), t, mParentAxis->mAxisRect->top())); + } + } else + { + // draw zeroline: + int zeroLineIndex = -1; + if (mZeroLinePen.style() != Qt::NoPen && mParentAxis->mRange.lower < 0 && mParentAxis->mRange.upper > 0) + { + applyAntialiasingHint(painter, mAntialiasedZeroLine, QCP::aeZeroLine); + painter->setPen(mZeroLinePen); + double epsilon = mParentAxis->mRange.size()*1E-6; // for comparing double to zero + for (int i=lowTick; i <= highTick; ++i) + { + if (qAbs(mParentAxis->mTickVector.at(i)) < epsilon) + { + zeroLineIndex = i; + t = mParentAxis->coordToPixel(mParentAxis->mTickVector.at(i)); // y + painter->drawLine(QLineF(mParentAxis->mAxisRect->left(), t, mParentAxis->mAxisRect->right(), t)); + break; + } + } + } + // draw grid lines: + applyDefaultAntialiasingHint(painter); + painter->setPen(mPen); + for (int i=lowTick; i <= highTick; ++i) + { + if (i == zeroLineIndex) continue; // don't draw a gridline on top of the zeroline + t = mParentAxis->coordToPixel(mParentAxis->mTickVector.at(i)); // y + painter->drawLine(QLineF(mParentAxis->mAxisRect->left(), t, mParentAxis->mAxisRect->right(), t)); + } + } +} + +/*! \internal + + Draws the sub grid lines with the specified painter. + + This is a helper function called by \ref draw. +*/ +void QCPGrid::drawSubGridLines(QCPPainter *painter) const +{ + if (!mParentAxis) { qDebug() << Q_FUNC_INFO << "invalid parent axis"; return; } + + applyAntialiasingHint(painter, mAntialiasedSubGrid, QCP::aeSubGrid); + double t; // helper variable, result of coordinate-to-pixel transforms + painter->setPen(mSubGridPen); + if (mParentAxis->orientation() == Qt::Horizontal) + { + for (int i=0; imSubTickVector.size(); ++i) + { + t = mParentAxis->coordToPixel(mParentAxis->mSubTickVector.at(i)); // x + painter->drawLine(QLineF(t, mParentAxis->mAxisRect->bottom(), t, mParentAxis->mAxisRect->top())); + } + } else + { + for (int i=0; imSubTickVector.size(); ++i) + { + t = mParentAxis->coordToPixel(mParentAxis->mSubTickVector.at(i)); // y + painter->drawLine(QLineF(mParentAxis->mAxisRect->left(), t, mParentAxis->mAxisRect->right(), t)); + } + } +} + + +//////////////////////////////////////////////////////////////////////////////////////////////////// +//////////////////// QCPAxis +//////////////////////////////////////////////////////////////////////////////////////////////////// + +/*! \class QCPAxis + \brief Manages a single axis inside a QCustomPlot. + + Usually doesn't need to be instantiated externally. Access %QCustomPlot's default four axes via + QCustomPlot::xAxis (bottom), QCustomPlot::yAxis (left), QCustomPlot::xAxis2 (top) and + QCustomPlot::yAxis2 (right). + + Axes are always part of an axis rect, see QCPAxisRect. + \image html AxisNamesOverview.png +
Naming convention of axis parts
+ \n + + \image html AxisRectSpacingOverview.png +
Overview of the spacings and paddings that define the geometry of an axis. The dashed gray line + on the left represents the QCustomPlot widget border.
+ +*/ + +/* start of documentation of inline functions */ + +/*! \fn Qt::Orientation QCPAxis::orientation() const + + Returns the orientation of this axis. The axis orientation (horizontal or vertical) is deduced + from the axis type (left, top, right or bottom). + + \see orientation(AxisType type) +*/ + +/*! \fn QCPGrid *QCPAxis::grid() const + + Returns the \ref QCPGrid instance belonging to this axis. Access it to set details about the way the + grid is displayed. +*/ + +/*! \fn static Qt::Orientation QCPAxis::orientation(AxisType type) + + Returns the orientation of the specified axis type + + \see orientation() +*/ + +/* end of documentation of inline functions */ +/* start of documentation of signals */ + +/*! \fn void QCPAxis::ticksRequest() + + This signal is emitted when \ref setAutoTicks is false and the axis is about to generate tick + labels for a replot. + + Modifying the tick positions can be done with \ref setTickVector. If you also want to control the + tick labels, set \ref setAutoTickLabels to false and also provide the labels with \ref + setTickVectorLabels. + + If you only want static ticks you probably don't need this signal, since you can just set the + tick vector (and possibly tick label vector) once. However, if you want to provide ticks (and + maybe labels) dynamically, e.g. depending on the current axis range, connect a slot to this + signal and set the vector/vectors there. +*/ + +/*! \fn void QCPAxis::rangeChanged(const QCPRange &newRange) + + This signal is emitted when the range of this axis has changed. You can connect it to the \ref + setRange slot of another axis to communicate the new range to the other axis, in order for it to + be synchronized. + + You may also manipulate/correct the range with \ref setRange in a slot connected to this signal. + This is useful if for example a maximum range span shall not be exceeded, or if the lower/upper + range shouldn't go beyond certain values. For example, the following slot would limit the x axis + to only positive ranges: + \code + if (newRange.lower < 0) + plot->xAxis->setRange(0, newRange.size()); + \endcode +*/ + +/*! \fn void QCPAxis::rangeChanged(const QCPRange &newRange, const QCPRange &oldRange) + \overload + + Additionally to the new range, this signal also provides the previous range held by the axis as + \a oldRange. +*/ + +/*! \fn void QCPAxis::scaleTypeChanged(QCPAxis::ScaleType scaleType); + + This signal is emitted when the scale type changes, by calls to \ref setScaleType +*/ + +/*! \fn void QCPAxis::selectionChanged(QCPAxis::SelectableParts selection) + + This signal is emitted when the selection state of this axis has changed, either by user interaction + or by a direct call to \ref setSelectedParts. +*/ + +/*! \fn void QCPAxis::selectableChanged(const QCPAxis::SelectableParts &parts); + + This signal is emitted when the selectability changes, by calls to \ref setSelectableParts +*/ + +/* end of documentation of signals */ + +/*! + Constructs an Axis instance of Type \a type for the axis rect \a parent. + + Usually it isn't necessary to instantiate axes directly, because you can let QCustomPlot create + them for you with \ref QCPAxisRect::addAxis. If you want to use own QCPAxis-subclasses however, + create them manually and then inject them also via \ref QCPAxisRect::addAxis. +*/ +QCPAxis::QCPAxis(QCPAxisRect *parent, AxisType type) : + QCPLayerable(parent->parentPlot(), QString(), parent), + // axis base: + mAxisType(type), + mAxisRect(parent), + mPadding(5), + mOrientation(orientation(type)), + mSelectableParts(spAxis | spTickLabels | spAxisLabel), + mSelectedParts(spNone), + mBasePen(QPen(Qt::black, 0, Qt::SolidLine, Qt::SquareCap)), + mSelectedBasePen(QPen(Qt::blue, 2)), + // axis label: + mLabel(), + mLabelFont(mParentPlot->font()), + mSelectedLabelFont(QFont(mLabelFont.family(), mLabelFont.pointSize(), QFont::Bold)), + mLabelColor(Qt::black), + mSelectedLabelColor(Qt::blue), + // tick labels: + mTickLabels(true), + mAutoTickLabels(true), + mTickLabelType(ltNumber), + mTickLabelFont(mParentPlot->font()), + mSelectedTickLabelFont(QFont(mTickLabelFont.family(), mTickLabelFont.pointSize(), QFont::Bold)), + mTickLabelColor(Qt::black), + mSelectedTickLabelColor(Qt::blue), + mDateTimeFormat(QLatin1String("hh:mm:ss\ndd.MM.yy")), + mDateTimeSpec(Qt::LocalTime), + mNumberPrecision(6), + mNumberFormatChar('g'), + mNumberBeautifulPowers(true), + // ticks and subticks: + mTicks(true), + mTickStep(1), + mSubTickCount(4), + mAutoTickCount(6), + mAutoTicks(true), + mAutoTickStep(true), + mAutoSubTicks(true), + mTickPen(QPen(Qt::black, 0, Qt::SolidLine, Qt::SquareCap)), + mSelectedTickPen(QPen(Qt::blue, 2)), + mSubTickPen(QPen(Qt::black, 0, Qt::SolidLine, Qt::SquareCap)), + mSelectedSubTickPen(QPen(Qt::blue, 2)), + // scale and range: + mRange(0, 5), + mRangeReversed(false), + mScaleType(stLinear), + mScaleLogBase(10), + mScaleLogBaseLogInv(1.0/qLn(mScaleLogBase)), + // internal members: + mGrid(new QCPGrid(this)), + mAxisPainter(new QCPAxisPainterPrivate(parent->parentPlot())), + mLowestVisibleTick(0), + mHighestVisibleTick(-1), + mCachedMarginValid(false), + mCachedMargin(0) +{ + setParent(parent); + mGrid->setVisible(false); + setAntialiased(false); + setLayer(mParentPlot->currentLayer()); // it's actually on that layer already, but we want it in front of the grid, so we place it on there again + + if (type == atTop) + { + setTickLabelPadding(3); + setLabelPadding(6); + } else if (type == atRight) + { + setTickLabelPadding(7); + setLabelPadding(12); + } else if (type == atBottom) + { + setTickLabelPadding(3); + setLabelPadding(3); + } else if (type == atLeft) + { + setTickLabelPadding(5); + setLabelPadding(10); + } +} + +QCPAxis::~QCPAxis() +{ + delete mAxisPainter; + delete mGrid; // delete grid here instead of via parent ~QObject for better defined deletion order +} + +/* No documentation as it is a property getter */ +int QCPAxis::tickLabelPadding() const +{ + return mAxisPainter->tickLabelPadding; +} + +/* No documentation as it is a property getter */ +double QCPAxis::tickLabelRotation() const +{ + return mAxisPainter->tickLabelRotation; +} + +/* No documentation as it is a property getter */ +QCPAxis::LabelSide QCPAxis::tickLabelSide() const +{ + return mAxisPainter->tickLabelSide; +} + +/* No documentation as it is a property getter */ +QString QCPAxis::numberFormat() const +{ + QString result; + result.append(mNumberFormatChar); + if (mNumberBeautifulPowers) + { + result.append(QLatin1Char('b')); + if (mAxisPainter->numberMultiplyCross) + result.append(QLatin1Char('c')); + } + return result; +} + +/* No documentation as it is a property getter */ +int QCPAxis::tickLengthIn() const +{ + return mAxisPainter->tickLengthIn; +} + +/* No documentation as it is a property getter */ +int QCPAxis::tickLengthOut() const +{ + return mAxisPainter->tickLengthOut; +} + +/* No documentation as it is a property getter */ +int QCPAxis::subTickLengthIn() const +{ + return mAxisPainter->subTickLengthIn; +} + +/* No documentation as it is a property getter */ +int QCPAxis::subTickLengthOut() const +{ + return mAxisPainter->subTickLengthOut; +} + +/* No documentation as it is a property getter */ +int QCPAxis::labelPadding() const +{ + return mAxisPainter->labelPadding; +} + +/* No documentation as it is a property getter */ +int QCPAxis::offset() const +{ + return mAxisPainter->offset; +} + +/* No documentation as it is a property getter */ +QCPLineEnding QCPAxis::lowerEnding() const +{ + return mAxisPainter->lowerEnding; +} + +/* No documentation as it is a property getter */ +QCPLineEnding QCPAxis::upperEnding() const +{ + return mAxisPainter->upperEnding; +} + +/*! + Sets whether the axis uses a linear scale or a logarithmic scale. If \a type is set to \ref + stLogarithmic, the logarithm base can be set with \ref setScaleLogBase. In logarithmic axis + scaling, major tick marks appear at all powers of the logarithm base. Properties like tick step + (\ref setTickStep) don't apply in logarithmic scaling. If you wish a decimal base but less major + ticks, consider choosing a logarithm base of 100, 1000 or even higher. + + If \a type is \ref stLogarithmic and the number format (\ref setNumberFormat) uses the 'b' option + (beautifully typeset decimal powers), the display usually is "1 [multiplication sign] 10 + [superscript] n", which looks unnatural for logarithmic scaling (the "1 [multiplication sign]" + part). To only display the decimal power, set the number precision to zero with + \ref setNumberPrecision. +*/ +void QCPAxis::setScaleType(QCPAxis::ScaleType type) +{ + if (mScaleType != type) + { + mScaleType = type; + if (mScaleType == stLogarithmic) + setRange(mRange.sanitizedForLogScale()); + mCachedMarginValid = false; + emit scaleTypeChanged(mScaleType); + } +} + +/*! + If \ref setScaleType is set to \ref stLogarithmic, \a base will be the logarithm base of the + scaling. In logarithmic axis scaling, major tick marks appear at all powers of \a base. + + Properties like tick step (\ref setTickStep) don't apply in logarithmic scaling. If you wish a decimal base but + less major ticks, consider choosing \a base 100, 1000 or even higher. +*/ +void QCPAxis::setScaleLogBase(double base) +{ + if (base > 1) + { + mScaleLogBase = base; + mScaleLogBaseLogInv = 1.0/qLn(mScaleLogBase); // buffer for faster baseLog() calculation + mCachedMarginValid = false; + } else + qDebug() << Q_FUNC_INFO << "Invalid logarithmic scale base (must be greater 1):" << base; +} + +/*! + Sets the range of the axis. + + This slot may be connected with the \ref rangeChanged signal of another axis so this axis + is always synchronized with the other axis range, when it changes. + + To invert the direction of an axis, use \ref setRangeReversed. +*/ +void QCPAxis::setRange(const QCPRange &range) +{ + if (range.lower == mRange.lower && range.upper == mRange.upper) + return; + + if (!QCPRange::validRange(range)) return; + QCPRange oldRange = mRange; + if (mScaleType == stLogarithmic) + { + mRange = range.sanitizedForLogScale(); + } else + { + mRange = range.sanitizedForLinScale(); + } + mCachedMarginValid = false; + emit rangeChanged(mRange); + emit rangeChanged(mRange, oldRange); +} + +/*! + Sets whether the user can (de-)select the parts in \a selectable by clicking on the QCustomPlot surface. + (When \ref QCustomPlot::setInteractions contains iSelectAxes.) + + However, even when \a selectable is set to a value not allowing the selection of a specific part, + it is still possible to set the selection of this part manually, by calling \ref setSelectedParts + directly. + + \see SelectablePart, setSelectedParts +*/ +void QCPAxis::setSelectableParts(const SelectableParts &selectable) +{ + if (mSelectableParts != selectable) + { + mSelectableParts = selectable; + emit selectableChanged(mSelectableParts); + } +} + +/*! + Sets the selected state of the respective axis parts described by \ref SelectablePart. When a part + is selected, it uses a different pen/font. + + The entire selection mechanism for axes is handled automatically when \ref + QCustomPlot::setInteractions contains iSelectAxes. You only need to call this function when you + wish to change the selection state manually. + + This function can change the selection state of a part, independent of the \ref setSelectableParts setting. + + emits the \ref selectionChanged signal when \a selected is different from the previous selection state. + + \see SelectablePart, setSelectableParts, selectTest, setSelectedBasePen, setSelectedTickPen, setSelectedSubTickPen, + setSelectedTickLabelFont, setSelectedLabelFont, setSelectedTickLabelColor, setSelectedLabelColor +*/ +void QCPAxis::setSelectedParts(const SelectableParts &selected) +{ + if (mSelectedParts != selected) + { + mSelectedParts = selected; + emit selectionChanged(mSelectedParts); + } +} + +/*! + \overload + + Sets the lower and upper bound of the axis range. + + To invert the direction of an axis, use \ref setRangeReversed. + + There is also a slot to set a range, see \ref setRange(const QCPRange &range). +*/ +void QCPAxis::setRange(double lower, double upper) +{ + if (lower == mRange.lower && upper == mRange.upper) + return; + + if (!QCPRange::validRange(lower, upper)) return; + QCPRange oldRange = mRange; + mRange.lower = lower; + mRange.upper = upper; + if (mScaleType == stLogarithmic) + { + mRange = mRange.sanitizedForLogScale(); + } else + { + mRange = mRange.sanitizedForLinScale(); + } + mCachedMarginValid = false; + emit rangeChanged(mRange); + emit rangeChanged(mRange, oldRange); +} + +/*! + \overload + + Sets the range of the axis. + + The \a position coordinate indicates together with the \a alignment parameter, where the new + range will be positioned. \a size defines the size of the new axis range. \a alignment may be + Qt::AlignLeft, Qt::AlignRight or Qt::AlignCenter. This will cause the left border, right border, + or center of the range to be aligned with \a position. Any other values of \a alignment will + default to Qt::AlignCenter. +*/ +void QCPAxis::setRange(double position, double size, Qt::AlignmentFlag alignment) +{ + if (alignment == Qt::AlignLeft) + setRange(position, position+size); + else if (alignment == Qt::AlignRight) + setRange(position-size, position); + else // alignment == Qt::AlignCenter + setRange(position-size/2.0, position+size/2.0); +} + +/*! + Sets the lower bound of the axis range. The upper bound is not changed. + \see setRange +*/ +void QCPAxis::setRangeLower(double lower) +{ + if (mRange.lower == lower) + return; + + QCPRange oldRange = mRange; + mRange.lower = lower; + if (mScaleType == stLogarithmic) + { + mRange = mRange.sanitizedForLogScale(); + } else + { + mRange = mRange.sanitizedForLinScale(); + } + mCachedMarginValid = false; + emit rangeChanged(mRange); + emit rangeChanged(mRange, oldRange); +} + +/*! + Sets the upper bound of the axis range. The lower bound is not changed. + \see setRange +*/ +void QCPAxis::setRangeUpper(double upper) +{ + if (mRange.upper == upper) + return; + + QCPRange oldRange = mRange; + mRange.upper = upper; + if (mScaleType == stLogarithmic) + { + mRange = mRange.sanitizedForLogScale(); + } else + { + mRange = mRange.sanitizedForLinScale(); + } + mCachedMarginValid = false; + emit rangeChanged(mRange); + emit rangeChanged(mRange, oldRange); +} + +/*! + Sets whether the axis range (direction) is displayed reversed. Normally, the values on horizontal + axes increase left to right, on vertical axes bottom to top. When \a reversed is set to true, the + direction of increasing values is inverted. + + Note that the range and data interface stays the same for reversed axes, e.g. the \a lower part + of the \ref setRange interface will still reference the mathematically smaller number than the \a + upper part. +*/ +void QCPAxis::setRangeReversed(bool reversed) +{ + if (mRangeReversed != reversed) + { + mRangeReversed = reversed; + mCachedMarginValid = false; + } +} + +/*! + Sets whether the tick positions should be calculated automatically (either from an automatically + generated tick step or a tick step provided manually via \ref setTickStep, see \ref setAutoTickStep). + + If \a on is set to false, you must provide the tick positions manually via \ref setTickVector. + For these manual ticks you may let QCPAxis generate the appropriate labels automatically by + leaving \ref setAutoTickLabels set to true. If you also wish to control the displayed labels + manually, set \ref setAutoTickLabels to false and provide the label strings with \ref + setTickVectorLabels. + + If you need dynamically calculated tick vectors (and possibly tick label vectors), set the + vectors in a slot connected to the \ref ticksRequest signal. + + \see setAutoTickLabels, setAutoSubTicks, setAutoTickCount, setAutoTickStep +*/ +void QCPAxis::setAutoTicks(bool on) +{ + if (mAutoTicks != on) + { + mAutoTicks = on; + mCachedMarginValid = false; + } +} + +/*! + When \ref setAutoTickStep is true, \a approximateCount determines how many ticks should be + generated in the visible range, approximately. + + It's not guaranteed that this number of ticks is met exactly, but approximately within a + tolerance of about two. + + Only values greater than zero are accepted as \a approximateCount. + + \see setAutoTickStep, setAutoTicks, setAutoSubTicks +*/ +void QCPAxis::setAutoTickCount(int approximateCount) +{ + if (mAutoTickCount != approximateCount) + { + if (approximateCount > 0) + { + mAutoTickCount = approximateCount; + mCachedMarginValid = false; + } else + qDebug() << Q_FUNC_INFO << "approximateCount must be greater than zero:" << approximateCount; + } +} + +/*! + Sets whether the tick labels are generated automatically. Depending on the tick label type (\ref + ltNumber or \ref ltDateTime), the labels will either show the coordinate as floating point + number (\ref setNumberFormat), or a date/time formatted according to \ref setDateTimeFormat. + + If \a on is set to false, you should provide the tick labels via \ref setTickVectorLabels. This + is usually used in a combination with \ref setAutoTicks set to false for complete control over + tick positions and labels, e.g. when the ticks should be at multiples of pi and show "2pi", "3pi" + etc. as tick labels. + + If you need dynamically calculated tick vectors (and possibly tick label vectors), set the + vectors in a slot connected to the \ref ticksRequest signal. + + \see setAutoTicks +*/ +void QCPAxis::setAutoTickLabels(bool on) +{ + if (mAutoTickLabels != on) + { + mAutoTickLabels = on; + mCachedMarginValid = false; + } +} + +/*! + Sets whether the tick step, i.e. the interval between two (major) ticks, is calculated + automatically. If \a on is set to true, the axis finds a tick step that is reasonable for human + readable plots. + + The number of ticks the algorithm aims for within the visible range can be specified with \ref + setAutoTickCount. + + If \a on is set to false, you may set the tick step manually with \ref setTickStep. + + \see setAutoTicks, setAutoSubTicks, setAutoTickCount +*/ +void QCPAxis::setAutoTickStep(bool on) +{ + if (mAutoTickStep != on) + { + mAutoTickStep = on; + mCachedMarginValid = false; + } +} + +/*! + Sets whether the number of sub ticks in one tick interval is determined automatically. This + works, as long as the tick step mantissa is a multiple of 0.5. When \ref setAutoTickStep is + enabled, this is always the case. + + When \a on is set to false, you may set the sub tick count with \ref setSubTickCount manually. + + \see setAutoTickCount, setAutoTicks, setAutoTickStep +*/ +void QCPAxis::setAutoSubTicks(bool on) +{ + if (mAutoSubTicks != on) + { + mAutoSubTicks = on; + mCachedMarginValid = false; + } +} + +/*! + Sets whether tick marks are displayed. + + Note that setting \a show to false does not imply that tick labels are invisible, too. To achieve + that, see \ref setTickLabels. +*/ +void QCPAxis::setTicks(bool show) +{ + if (mTicks != show) + { + mTicks = show; + mCachedMarginValid = false; + } +} + +/*! + Sets whether tick labels are displayed. Tick labels are the numbers drawn next to tick marks. +*/ +void QCPAxis::setTickLabels(bool show) +{ + if (mTickLabels != show) + { + mTickLabels = show; + mCachedMarginValid = false; + } +} + +/*! + Sets the distance between the axis base line (including any outward ticks) and the tick labels. + \see setLabelPadding, setPadding +*/ +void QCPAxis::setTickLabelPadding(int padding) +{ + if (mAxisPainter->tickLabelPadding != padding) + { + mAxisPainter->tickLabelPadding = padding; + mCachedMarginValid = false; + } +} + +/*! + Sets whether the tick labels display numbers or dates/times. + + If \a type is set to \ref ltNumber, the format specifications of \ref setNumberFormat apply. + + If \a type is set to \ref ltDateTime, the format specifications of \ref setDateTimeFormat apply. + + In QCustomPlot, date/time coordinates are double numbers representing the seconds since + 1970-01-01T00:00:00 UTC. This format can be retrieved from QDateTime objects with the + QDateTime::toTime_t() function. Since this only gives a resolution of one second, there is also + the QDateTime::toMSecsSinceEpoch() function which returns the timespan described above in + milliseconds. Divide its return value by 1000.0 to get a value with the format needed for + date/time plotting, with a resolution of one millisecond. + + Using the toMSecsSinceEpoch function allows dates that go back to 2nd January 4713 B.C. + (represented by a negative number), unlike the toTime_t function, which works with unsigned + integers and thus only goes back to 1st January 1970. So both for range and accuracy, use of + toMSecsSinceEpoch()/1000.0 should be preferred as key coordinate for date/time axes. + + \see setTickLabels +*/ +void QCPAxis::setTickLabelType(LabelType type) +{ + if (mTickLabelType != type) + { + mTickLabelType = type; + mCachedMarginValid = false; + } +} + +/*! + Sets the font of the tick labels. + + \see setTickLabels, setTickLabelColor +*/ +void QCPAxis::setTickLabelFont(const QFont &font) +{ + if (font != mTickLabelFont) + { + mTickLabelFont = font; + mCachedMarginValid = false; + } +} + +/*! + Sets the color of the tick labels. + + \see setTickLabels, setTickLabelFont +*/ +void QCPAxis::setTickLabelColor(const QColor &color) +{ + if (color != mTickLabelColor) + { + mTickLabelColor = color; + mCachedMarginValid = false; + } +} + +/*! + Sets the rotation of the tick labels. If \a degrees is zero, the labels are drawn normally. Else, + the tick labels are drawn rotated by \a degrees clockwise. The specified angle is bound to values + from -90 to 90 degrees. + + If \a degrees is exactly -90, 0 or 90, the tick labels are centered on the tick coordinate. For + other angles, the label is drawn with an offset such that it seems to point toward or away from + the tick mark. +*/ +void QCPAxis::setTickLabelRotation(double degrees) +{ + if (!qFuzzyIsNull(degrees-mAxisPainter->tickLabelRotation)) + { + mAxisPainter->tickLabelRotation = qBound(-90.0, degrees, 90.0); + mCachedMarginValid = false; + } +} + +/*! + Sets whether the tick labels (numbers) shall appear inside or outside the axis rect. + + The usual and default setting is \ref lsOutside. Very compact plots sometimes require tick labels + to be inside the axis rect, to save space. If \a side is set to \ref lsInside, the tick labels + appear on the inside are additionally clipped to the axis rect. +*/ +void QCPAxis::setTickLabelSide(LabelSide side) +{ + mAxisPainter->tickLabelSide = side; + mCachedMarginValid = false; +} + +/*! + Sets the format in which dates and times are displayed as tick labels, if \ref setTickLabelType is \ref ltDateTime. + for details about the \a format string, see the documentation of QDateTime::toString(). + + Newlines can be inserted with "\n". + + \see setDateTimeSpec +*/ +void QCPAxis::setDateTimeFormat(const QString &format) +{ + if (mDateTimeFormat != format) + { + mDateTimeFormat = format; + mCachedMarginValid = false; + } +} + +/*! + Sets the time spec that is used for the date time values when \ref setTickLabelType is \ref + ltDateTime. + + The default value of QDateTime objects (and also QCustomPlot) is Qt::LocalTime. However, + if the date time values passed to QCustomPlot are given in the UTC spec, set \a + timeSpec to Qt::UTC to get the correct axis labels. + + \see setDateTimeFormat +*/ +void QCPAxis::setDateTimeSpec(const Qt::TimeSpec &timeSpec) +{ + mDateTimeSpec = timeSpec; +} + +/*! + Sets the number format for the numbers drawn as tick labels (if tick label type is \ref + ltNumber). This \a formatCode is an extended version of the format code used e.g. by + QString::number() and QLocale::toString(). For reference about that, see the "Argument Formats" + section in the detailed description of the QString class. \a formatCode is a string of one, two + or three characters. The first character is identical to the normal format code used by Qt. In + short, this means: 'e'/'E' scientific format, 'f' fixed format, 'g'/'G' scientific or fixed, + whichever is shorter. + + The second and third characters are optional and specific to QCustomPlot:\n + If the first char was 'e' or 'g', numbers are/might be displayed in the scientific format, e.g. + "5.5e9", which is ugly in a plot. So when the second char of \a formatCode is set to 'b' (for + "beautiful"), those exponential numbers are formatted in a more natural way, i.e. "5.5 + [multiplication sign] 10 [superscript] 9". By default, the multiplication sign is a centered dot. + If instead a cross should be shown (as is usual in the USA), the third char of \a formatCode can + be set to 'c'. The inserted multiplication signs are the UTF-8 characters 215 (0xD7) for the + cross and 183 (0xB7) for the dot. + + If the scale type (\ref setScaleType) is \ref stLogarithmic and the \a formatCode uses the 'b' + option (beautifully typeset decimal powers), the display usually is "1 [multiplication sign] 10 + [superscript] n", which looks unnatural for logarithmic scaling (the "1 [multiplication sign]" + part). To only display the decimal power, set the number precision to zero with \ref + setNumberPrecision. + + Examples for \a formatCode: + \li \c g normal format code behaviour. If number is small, fixed format is used, if number is large, + normal scientific format is used + \li \c gb If number is small, fixed format is used, if number is large, scientific format is used with + beautifully typeset decimal powers and a dot as multiplication sign + \li \c ebc All numbers are in scientific format with beautifully typeset decimal power and a cross as + multiplication sign + \li \c fb illegal format code, since fixed format doesn't support (or need) beautifully typeset decimal + powers. Format code will be reduced to 'f'. + \li \c hello illegal format code, since first char is not 'e', 'E', 'f', 'g' or 'G'. Current format + code will not be changed. +*/ +void QCPAxis::setNumberFormat(const QString &formatCode) +{ + if (formatCode.isEmpty()) + { + qDebug() << Q_FUNC_INFO << "Passed formatCode is empty"; + return; + } + mCachedMarginValid = false; + + // interpret first char as number format char: + QString allowedFormatChars(QLatin1String("eEfgG")); + if (allowedFormatChars.contains(formatCode.at(0))) + { + mNumberFormatChar = QLatin1Char(formatCode.at(0).toLatin1()); + } else + { + qDebug() << Q_FUNC_INFO << "Invalid number format code (first char not in 'eEfgG'):" << formatCode; + return; + } + if (formatCode.length() < 2) + { + mNumberBeautifulPowers = false; + mAxisPainter->numberMultiplyCross = false; + return; + } + + // interpret second char as indicator for beautiful decimal powers: + if (formatCode.at(1) == QLatin1Char('b') && (mNumberFormatChar == QLatin1Char('e') || mNumberFormatChar == QLatin1Char('g'))) + { + mNumberBeautifulPowers = true; + } else + { + qDebug() << Q_FUNC_INFO << "Invalid number format code (second char not 'b' or first char neither 'e' nor 'g'):" << formatCode; + return; + } + if (formatCode.length() < 3) + { + mAxisPainter->numberMultiplyCross = false; + return; + } + + // interpret third char as indicator for dot or cross multiplication symbol: + if (formatCode.at(2) == QLatin1Char('c')) + { + mAxisPainter->numberMultiplyCross = true; + } else if (formatCode.at(2) == QLatin1Char('d')) + { + mAxisPainter->numberMultiplyCross = false; + } else + { + qDebug() << Q_FUNC_INFO << "Invalid number format code (third char neither 'c' nor 'd'):" << formatCode; + return; + } +} + +/*! + Sets the precision of the tick label numbers. See QLocale::toString(double i, char f, int prec) + for details. The effect of precisions are most notably for number Formats starting with 'e', see + \ref setNumberFormat + + If the scale type (\ref setScaleType) is \ref stLogarithmic and the number format (\ref + setNumberFormat) uses the 'b' format code (beautifully typeset decimal powers), the display + usually is "1 [multiplication sign] 10 [superscript] n", which looks unnatural for logarithmic + scaling (the redundant "1 [multiplication sign]" part). To only display the decimal power "10 + [superscript] n", set \a precision to zero. +*/ +void QCPAxis::setNumberPrecision(int precision) +{ + if (mNumberPrecision != precision) + { + mNumberPrecision = precision; + mCachedMarginValid = false; + } +} + +/*! + If \ref setAutoTickStep is set to false, use this function to set the tick step manually. + The tick step is the interval between (major) ticks, in plot coordinates. + \see setSubTickCount +*/ +void QCPAxis::setTickStep(double step) +{ + if (mTickStep != step) + { + mTickStep = step; + mCachedMarginValid = false; + } +} + +/*! + If you want full control over what ticks (and possibly labels) the axes show, this function is + used to set the coordinates at which ticks will appear.\ref setAutoTicks must be disabled, else + the provided tick vector will be overwritten with automatically generated tick coordinates upon + replot. The labels of the ticks can be generated automatically when \ref setAutoTickLabels is + left enabled. If it is disabled, you can set the labels manually with \ref setTickVectorLabels. + + \a vec is a vector containing the positions of the ticks, in plot coordinates. + + \warning \a vec must be sorted in ascending order, no additional checks are made to ensure this. + + \see setTickVectorLabels +*/ +void QCPAxis::setTickVector(const QVector &vec) +{ + // don't check whether mTickVector != vec here, because it takes longer than we would save + mTickVector = vec; + mCachedMarginValid = false; +} + +/*! + If you want full control over what ticks and labels the axes show, this function is used to set a + number of QStrings that will be displayed at the tick positions which you need to provide with + \ref setTickVector. These two vectors should have the same size. (Note that you need to disable + \ref setAutoTicks and \ref setAutoTickLabels first.) + + \a vec is a vector containing the labels of the ticks. The entries correspond to the respective + indices in the tick vector, passed via \ref setTickVector. + + \see setTickVector +*/ +void QCPAxis::setTickVectorLabels(const QVector &vec) +{ + // don't check whether mTickVectorLabels != vec here, because it takes longer than we would save + mTickVectorLabels = vec; + mCachedMarginValid = false; +} + +/*! + Sets the length of the ticks in pixels. \a inside is the length the ticks will reach inside the + plot and \a outside is the length they will reach outside the plot. If \a outside is greater than + zero, the tick labels and axis label will increase their distance to the axis accordingly, so + they won't collide with the ticks. + + \see setSubTickLength, setTickLengthIn, setTickLengthOut +*/ +void QCPAxis::setTickLength(int inside, int outside) +{ + setTickLengthIn(inside); + setTickLengthOut(outside); +} + +/*! + Sets the length of the inward ticks in pixels. \a inside is the length the ticks will reach + inside the plot. + + \see setTickLengthOut, setTickLength, setSubTickLength +*/ +void QCPAxis::setTickLengthIn(int inside) +{ + if (mAxisPainter->tickLengthIn != inside) + { + mAxisPainter->tickLengthIn = inside; + } +} + +/*! + Sets the length of the outward ticks in pixels. \a outside is the length the ticks will reach + outside the plot. If \a outside is greater than zero, the tick labels and axis label will + increase their distance to the axis accordingly, so they won't collide with the ticks. + + \see setTickLengthIn, setTickLength, setSubTickLength +*/ +void QCPAxis::setTickLengthOut(int outside) +{ + if (mAxisPainter->tickLengthOut != outside) + { + mAxisPainter->tickLengthOut = outside; + mCachedMarginValid = false; // only outside tick length can change margin + } +} + +/*! + Sets the number of sub ticks in one (major) tick step. A sub tick count of three for example, + divides the tick intervals in four sub intervals. + + By default, the number of sub ticks is chosen automatically in a reasonable manner as long as the + mantissa of the tick step is a multiple of 0.5. When \ref setAutoTickStep is enabled, this is + always the case. + + If you want to disable automatic sub tick count and use this function to set the count manually, + see \ref setAutoSubTicks. +*/ +void QCPAxis::setSubTickCount(int count) +{ + mSubTickCount = count; +} + +/*! + Sets the length of the subticks in pixels. \a inside is the length the subticks will reach inside + the plot and \a outside is the length they will reach outside the plot. If \a outside is greater + than zero, the tick labels and axis label will increase their distance to the axis accordingly, + so they won't collide with the ticks. + + \see setTickLength, setSubTickLengthIn, setSubTickLengthOut +*/ +void QCPAxis::setSubTickLength(int inside, int outside) +{ + setSubTickLengthIn(inside); + setSubTickLengthOut(outside); +} + +/*! + Sets the length of the inward subticks in pixels. \a inside is the length the subticks will reach inside + the plot. + + \see setSubTickLengthOut, setSubTickLength, setTickLength +*/ +void QCPAxis::setSubTickLengthIn(int inside) +{ + if (mAxisPainter->subTickLengthIn != inside) + { + mAxisPainter->subTickLengthIn = inside; + } +} + +/*! + Sets the length of the outward subticks in pixels. \a outside is the length the subticks will reach + outside the plot. If \a outside is greater than zero, the tick labels will increase their + distance to the axis accordingly, so they won't collide with the ticks. + + \see setSubTickLengthIn, setSubTickLength, setTickLength +*/ +void QCPAxis::setSubTickLengthOut(int outside) +{ + if (mAxisPainter->subTickLengthOut != outside) + { + mAxisPainter->subTickLengthOut = outside; + mCachedMarginValid = false; // only outside tick length can change margin + } +} + +/*! + Sets the pen, the axis base line is drawn with. + + \see setTickPen, setSubTickPen +*/ +void QCPAxis::setBasePen(const QPen &pen) +{ + mBasePen = pen; +} + +/*! + Sets the pen, tick marks will be drawn with. + + \see setTickLength, setBasePen +*/ +void QCPAxis::setTickPen(const QPen &pen) +{ + mTickPen = pen; +} + +/*! + Sets the pen, subtick marks will be drawn with. + + \see setSubTickCount, setSubTickLength, setBasePen +*/ +void QCPAxis::setSubTickPen(const QPen &pen) +{ + mSubTickPen = pen; +} + +/*! + Sets the font of the axis label. + + \see setLabelColor +*/ +void QCPAxis::setLabelFont(const QFont &font) +{ + if (mLabelFont != font) + { + mLabelFont = font; + mCachedMarginValid = false; + } +} + +/*! + Sets the color of the axis label. + + \see setLabelFont +*/ +void QCPAxis::setLabelColor(const QColor &color) +{ + mLabelColor = color; +} + +/*! + Sets the text of the axis label that will be shown below/above or next to the axis, depending on + its orientation. To disable axis labels, pass an empty string as \a str. +*/ +void QCPAxis::setLabel(const QString &str) +{ + if (mLabel != str) + { + mLabel = str; + mCachedMarginValid = false; + } +} + +/*! + Sets the distance between the tick labels and the axis label. + + \see setTickLabelPadding, setPadding +*/ +void QCPAxis::setLabelPadding(int padding) +{ + if (mAxisPainter->labelPadding != padding) + { + mAxisPainter->labelPadding = padding; + mCachedMarginValid = false; + } +} + +/*! + Sets the padding of the axis. + + When \ref QCPAxisRect::setAutoMargins is enabled, the padding is the additional outer most space, + that is left blank. + + The axis padding has no meaning if \ref QCPAxisRect::setAutoMargins is disabled. + + \see setLabelPadding, setTickLabelPadding +*/ +void QCPAxis::setPadding(int padding) +{ + if (mPadding != padding) + { + mPadding = padding; + mCachedMarginValid = false; + } +} + +/*! + Sets the offset the axis has to its axis rect side. + + If an axis rect side has multiple axes and automatic margin calculation is enabled for that side, + only the offset of the inner most axis has meaning (even if it is set to be invisible). The + offset of the other, outer axes is controlled automatically, to place them at appropriate + positions. +*/ +void QCPAxis::setOffset(int offset) +{ + mAxisPainter->offset = offset; +} + +/*! + Sets the font that is used for tick labels when they are selected. + + \see setTickLabelFont, setSelectableParts, setSelectedParts, QCustomPlot::setInteractions +*/ +void QCPAxis::setSelectedTickLabelFont(const QFont &font) +{ + if (font != mSelectedTickLabelFont) + { + mSelectedTickLabelFont = font; + // don't set mCachedMarginValid to false here because margin calculation is always done with non-selected fonts + } +} + +/*! + Sets the font that is used for the axis label when it is selected. + + \see setLabelFont, setSelectableParts, setSelectedParts, QCustomPlot::setInteractions +*/ +void QCPAxis::setSelectedLabelFont(const QFont &font) +{ + mSelectedLabelFont = font; + // don't set mCachedMarginValid to false here because margin calculation is always done with non-selected fonts +} + +/*! + Sets the color that is used for tick labels when they are selected. + + \see setTickLabelColor, setSelectableParts, setSelectedParts, QCustomPlot::setInteractions +*/ +void QCPAxis::setSelectedTickLabelColor(const QColor &color) +{ + if (color != mSelectedTickLabelColor) + { + mSelectedTickLabelColor = color; + } +} + +/*! + Sets the color that is used for the axis label when it is selected. + + \see setLabelColor, setSelectableParts, setSelectedParts, QCustomPlot::setInteractions +*/ +void QCPAxis::setSelectedLabelColor(const QColor &color) +{ + mSelectedLabelColor = color; +} + +/*! + Sets the pen that is used to draw the axis base line when selected. + + \see setBasePen, setSelectableParts, setSelectedParts, QCustomPlot::setInteractions +*/ +void QCPAxis::setSelectedBasePen(const QPen &pen) +{ + mSelectedBasePen = pen; +} + +/*! + Sets the pen that is used to draw the (major) ticks when selected. + + \see setTickPen, setSelectableParts, setSelectedParts, QCustomPlot::setInteractions +*/ +void QCPAxis::setSelectedTickPen(const QPen &pen) +{ + mSelectedTickPen = pen; +} + +/*! + Sets the pen that is used to draw the subticks when selected. + + \see setSubTickPen, setSelectableParts, setSelectedParts, QCustomPlot::setInteractions +*/ +void QCPAxis::setSelectedSubTickPen(const QPen &pen) +{ + mSelectedSubTickPen = pen; +} + +/*! + Sets the style for the lower axis ending. See the documentation of QCPLineEnding for available + styles. + + For horizontal axes, this method refers to the left ending, for vertical axes the bottom ending. + Note that this meaning does not change when the axis range is reversed with \ref + setRangeReversed. + + \see setUpperEnding +*/ +void QCPAxis::setLowerEnding(const QCPLineEnding &ending) +{ + mAxisPainter->lowerEnding = ending; +} + +/*! + Sets the style for the upper axis ending. See the documentation of QCPLineEnding for available + styles. + + For horizontal axes, this method refers to the right ending, for vertical axes the top ending. + Note that this meaning does not change when the axis range is reversed with \ref + setRangeReversed. + + \see setLowerEnding +*/ +void QCPAxis::setUpperEnding(const QCPLineEnding &ending) +{ + mAxisPainter->upperEnding = ending; +} + +/*! + If the scale type (\ref setScaleType) is \ref stLinear, \a diff is added to the lower and upper + bounds of the range. The range is simply moved by \a diff. + + If the scale type is \ref stLogarithmic, the range bounds are multiplied by \a diff. This + corresponds to an apparent "linear" move in logarithmic scaling by a distance of log(diff). +*/ +void QCPAxis::moveRange(double diff) +{ + QCPRange oldRange = mRange; + if (mScaleType == stLinear) + { + mRange.lower += diff; + mRange.upper += diff; + } else // mScaleType == stLogarithmic + { + mRange.lower *= diff; + mRange.upper *= diff; + } + mCachedMarginValid = false; + emit rangeChanged(mRange); + emit rangeChanged(mRange, oldRange); +} + +/*! + Scales the range of this axis by \a factor around the coordinate \a center. For example, if \a + factor is 2.0, \a center is 1.0, then the axis range will double its size, and the point at + coordinate 1.0 won't have changed its position in the QCustomPlot widget (i.e. coordinates + around 1.0 will have moved symmetrically closer to 1.0). +*/ +void QCPAxis::scaleRange(double factor, double center) +{ + QCPRange oldRange = mRange; + if (mScaleType == stLinear) + { + QCPRange newRange; + newRange.lower = (mRange.lower-center)*factor + center; + newRange.upper = (mRange.upper-center)*factor + center; + if (QCPRange::validRange(newRange)) + mRange = newRange.sanitizedForLinScale(); + } else // mScaleType == stLogarithmic + { + if ((mRange.upper < 0 && center < 0) || (mRange.upper > 0 && center > 0)) // make sure center has same sign as range + { + QCPRange newRange; + newRange.lower = qPow(mRange.lower/center, factor)*center; + newRange.upper = qPow(mRange.upper/center, factor)*center; + if (QCPRange::validRange(newRange)) + mRange = newRange.sanitizedForLogScale(); + } else + qDebug() << Q_FUNC_INFO << "Center of scaling operation doesn't lie in same logarithmic sign domain as range:" << center; + } + mCachedMarginValid = false; + emit rangeChanged(mRange); + emit rangeChanged(mRange, oldRange); +} + +/*! + Scales the range of this axis to have a certain scale \a ratio to \a otherAxis. The scaling will + be done around the center of the current axis range. + + For example, if \a ratio is 1, this axis is the \a yAxis and \a otherAxis is \a xAxis, graphs + plotted with those axes will appear in a 1:1 aspect ratio, independent of the aspect ratio the + axis rect has. + + This is an operation that changes the range of this axis once, it doesn't fix the scale ratio + indefinitely. Note that calling this function in the constructor of the QCustomPlot's parent + won't have the desired effect, since the widget dimensions aren't defined yet, and a resizeEvent + will follow. +*/ +void QCPAxis::setScaleRatio(const QCPAxis *otherAxis, double ratio) +{ + int otherPixelSize, ownPixelSize; + + if (otherAxis->orientation() == Qt::Horizontal) + otherPixelSize = otherAxis->axisRect()->width(); + else + otherPixelSize = otherAxis->axisRect()->height(); + + if (orientation() == Qt::Horizontal) + ownPixelSize = axisRect()->width(); + else + ownPixelSize = axisRect()->height(); + + double newRangeSize = ratio*otherAxis->range().size()*ownPixelSize/(double)otherPixelSize; + setRange(range().center(), newRangeSize, Qt::AlignCenter); +} + +/*! + Changes the axis range such that all plottables associated with this axis are fully visible in + that dimension. + + \see QCPAbstractPlottable::rescaleAxes, QCustomPlot::rescaleAxes +*/ +void QCPAxis::rescale(bool onlyVisiblePlottables) +{ + QList p = plottables(); + QCPRange newRange; + bool haveRange = false; + for (int i=0; irealVisibility() && onlyVisiblePlottables) + continue; + QCPRange plottableRange; + bool currentFoundRange; + QCPAbstractPlottable::SignDomain signDomain = QCPAbstractPlottable::sdBoth; + if (mScaleType == stLogarithmic) + signDomain = (mRange.upper < 0 ? QCPAbstractPlottable::sdNegative : QCPAbstractPlottable::sdPositive); + if (p.at(i)->keyAxis() == this) + plottableRange = p.at(i)->getKeyRange(currentFoundRange, signDomain); + else + plottableRange = p.at(i)->getValueRange(currentFoundRange, signDomain); + if (currentFoundRange) + { + if (!haveRange) + newRange = plottableRange; + else + newRange.expand(plottableRange); + haveRange = true; + } + } + if (haveRange) + { + if (!QCPRange::validRange(newRange)) // likely due to range being zero (plottable has only constant data in this axis dimension), shift current range to at least center the plottable + { + double center = (newRange.lower+newRange.upper)*0.5; // upper and lower should be equal anyway, but just to make sure, incase validRange returned false for other reason + if (mScaleType == stLinear) + { + newRange.lower = center-mRange.size()/2.0; + newRange.upper = center+mRange.size()/2.0; + } else // mScaleType == stLogarithmic + { + newRange.lower = center/qSqrt(mRange.upper/mRange.lower); + newRange.upper = center*qSqrt(mRange.upper/mRange.lower); + } + } + setRange(newRange); + } +} + +/*! + Transforms \a value, in pixel coordinates of the QCustomPlot widget, to axis coordinates. +*/ +double QCPAxis::pixelToCoord(double value) const +{ + if (orientation() == Qt::Horizontal) + { + if (mScaleType == stLinear) + { + if (!mRangeReversed) + return (value-mAxisRect->left())/(double)mAxisRect->width()*mRange.size()+mRange.lower; + else + return -(value-mAxisRect->left())/(double)mAxisRect->width()*mRange.size()+mRange.upper; + } else // mScaleType == stLogarithmic + { + if (!mRangeReversed) + return qPow(mRange.upper/mRange.lower, (value-mAxisRect->left())/(double)mAxisRect->width())*mRange.lower; + else + return qPow(mRange.upper/mRange.lower, (mAxisRect->left()-value)/(double)mAxisRect->width())*mRange.upper; + } + } else // orientation() == Qt::Vertical + { + if (mScaleType == stLinear) + { + if (!mRangeReversed) + return (mAxisRect->bottom()-value)/(double)mAxisRect->height()*mRange.size()+mRange.lower; + else + return -(mAxisRect->bottom()-value)/(double)mAxisRect->height()*mRange.size()+mRange.upper; + } else // mScaleType == stLogarithmic + { + if (!mRangeReversed) + return qPow(mRange.upper/mRange.lower, (mAxisRect->bottom()-value)/(double)mAxisRect->height())*mRange.lower; + else + return qPow(mRange.upper/mRange.lower, (value-mAxisRect->bottom())/(double)mAxisRect->height())*mRange.upper; + } + } +} + +/*! + Transforms \a value, in coordinates of the axis, to pixel coordinates of the QCustomPlot widget. +*/ +double QCPAxis::coordToPixel(double value) const +{ + if (orientation() == Qt::Horizontal) + { + if (mScaleType == stLinear) + { + if (!mRangeReversed) + return (value-mRange.lower)/mRange.size()*mAxisRect->width()+mAxisRect->left(); + else + return (mRange.upper-value)/mRange.size()*mAxisRect->width()+mAxisRect->left(); + } else // mScaleType == stLogarithmic + { + if (value >= 0 && mRange.upper < 0) // invalid value for logarithmic scale, just draw it outside visible range + return !mRangeReversed ? mAxisRect->right()+200 : mAxisRect->left()-200; + else if (value <= 0 && mRange.upper > 0) // invalid value for logarithmic scale, just draw it outside visible range + return !mRangeReversed ? mAxisRect->left()-200 : mAxisRect->right()+200; + else + { + if (!mRangeReversed) + return baseLog(value/mRange.lower)/baseLog(mRange.upper/mRange.lower)*mAxisRect->width()+mAxisRect->left(); + else + return baseLog(mRange.upper/value)/baseLog(mRange.upper/mRange.lower)*mAxisRect->width()+mAxisRect->left(); + } + } + } else // orientation() == Qt::Vertical + { + if (mScaleType == stLinear) + { + if (!mRangeReversed) + return mAxisRect->bottom()-(value-mRange.lower)/mRange.size()*mAxisRect->height(); + else + return mAxisRect->bottom()-(mRange.upper-value)/mRange.size()*mAxisRect->height(); + } else // mScaleType == stLogarithmic + { + if (value >= 0 && mRange.upper < 0) // invalid value for logarithmic scale, just draw it outside visible range + return !mRangeReversed ? mAxisRect->top()-200 : mAxisRect->bottom()+200; + else if (value <= 0 && mRange.upper > 0) // invalid value for logarithmic scale, just draw it outside visible range + return !mRangeReversed ? mAxisRect->bottom()+200 : mAxisRect->top()-200; + else + { + if (!mRangeReversed) + return mAxisRect->bottom()-baseLog(value/mRange.lower)/baseLog(mRange.upper/mRange.lower)*mAxisRect->height(); + else + return mAxisRect->bottom()-baseLog(mRange.upper/value)/baseLog(mRange.upper/mRange.lower)*mAxisRect->height(); + } + } + } +} + +/*! + Returns the part of the axis that is hit by \a pos (in pixels). The return value of this function + is independent of the user-selectable parts defined with \ref setSelectableParts. Further, this + function does not change the current selection state of the axis. + + If the axis is not visible (\ref setVisible), this function always returns \ref spNone. + + \see setSelectedParts, setSelectableParts, QCustomPlot::setInteractions +*/ +QCPAxis::SelectablePart QCPAxis::getPartAt(const QPointF &pos) const +{ + if (!mVisible) + return spNone; + + if (mAxisPainter->axisSelectionBox().contains(pos.toPoint())) + return spAxis; + else if (mAxisPainter->tickLabelsSelectionBox().contains(pos.toPoint())) + return spTickLabels; + else if (mAxisPainter->labelSelectionBox().contains(pos.toPoint())) + return spAxisLabel; + else + return spNone; +} + +/* inherits documentation from base class */ +double QCPAxis::selectTest(const QPointF &pos, bool onlySelectable, QVariant *details) const +{ + if (!mParentPlot) return -1; + SelectablePart part = getPartAt(pos); + if ((onlySelectable && !mSelectableParts.testFlag(part)) || part == spNone) + return -1; + + if (details) + details->setValue(part); + return mParentPlot->selectionTolerance()*0.99; +} + +/*! + Returns a list of all the plottables that have this axis as key or value axis. + + If you are only interested in plottables of type QCPGraph, see \ref graphs. + + \see graphs, items +*/ +QList QCPAxis::plottables() const +{ + QList result; + if (!mParentPlot) return result; + + for (int i=0; imPlottables.size(); ++i) + { + if (mParentPlot->mPlottables.at(i)->keyAxis() == this ||mParentPlot->mPlottables.at(i)->valueAxis() == this) + result.append(mParentPlot->mPlottables.at(i)); + } + return result; +} + +/*! + Returns a list of all the graphs that have this axis as key or value axis. + + \see plottables, items +*/ +QList QCPAxis::graphs() const +{ + QList result; + if (!mParentPlot) return result; + + for (int i=0; imGraphs.size(); ++i) + { + if (mParentPlot->mGraphs.at(i)->keyAxis() == this || mParentPlot->mGraphs.at(i)->valueAxis() == this) + result.append(mParentPlot->mGraphs.at(i)); + } + return result; +} + +/*! + Returns a list of all the items that are associated with this axis. An item is considered + associated with an axis if at least one of its positions uses the axis as key or value axis. + + \see plottables, graphs +*/ +QList QCPAxis::items() const +{ + QList result; + if (!mParentPlot) return result; + + for (int itemId=0; itemIdmItems.size(); ++itemId) + { + QList positions = mParentPlot->mItems.at(itemId)->positions(); + for (int posId=0; posIdkeyAxis() == this || positions.at(posId)->valueAxis() == this) + { + result.append(mParentPlot->mItems.at(itemId)); + break; + } + } + } + return result; +} + +/*! + Transforms a margin side to the logically corresponding axis type. (QCP::msLeft to + QCPAxis::atLeft, QCP::msRight to QCPAxis::atRight, etc.) +*/ +QCPAxis::AxisType QCPAxis::marginSideToAxisType(QCP::MarginSide side) +{ + switch (side) + { + case QCP::msLeft: return atLeft; + case QCP::msRight: return atRight; + case QCP::msTop: return atTop; + case QCP::msBottom: return atBottom; + default: break; + } + qDebug() << Q_FUNC_INFO << "Invalid margin side passed:" << (int)side; + return atLeft; +} + +/*! + Returns the axis type that describes the opposite axis of an axis with the specified \a type. +*/ +QCPAxis::AxisType QCPAxis::opposite(QCPAxis::AxisType type) +{ + switch (type) + { + case atLeft: return atRight; break; + case atRight: return atLeft; break; + case atBottom: return atTop; break; + case atTop: return atBottom; break; + default: qDebug() << Q_FUNC_INFO << "invalid axis type"; return atLeft; break; + } +} + +/*! \internal + + This function is called to prepare the tick vector, sub tick vector and tick label vector. If + \ref setAutoTicks is set to true, appropriate tick values are determined automatically via \ref + generateAutoTicks. If it's set to false, the signal ticksRequest is emitted, which can be used to + provide external tick positions. Then the sub tick vectors and tick label vectors are created. +*/ +void QCPAxis::setupTickVectors() +{ + if (!mParentPlot) return; + if ((!mTicks && !mTickLabels && !mGrid->visible()) || mRange.size() <= 0) return; + + // fill tick vectors, either by auto generating or by notifying user to fill the vectors himself + if (mAutoTicks) + { + generateAutoTicks(); + } else + { + emit ticksRequest(); + } + + visibleTickBounds(mLowestVisibleTick, mHighestVisibleTick); + if (mTickVector.isEmpty()) + { + mSubTickVector.clear(); + return; + } + + // generate subticks between ticks: + mSubTickVector.resize((mTickVector.size()-1)*mSubTickCount); + if (mSubTickCount > 0) + { + double subTickStep = 0; + double subTickPosition = 0; + int subTickIndex = 0; + bool done = false; + int lowTick = mLowestVisibleTick > 0 ? mLowestVisibleTick-1 : mLowestVisibleTick; + int highTick = mHighestVisibleTick < mTickVector.size()-1 ? mHighestVisibleTick+1 : mHighestVisibleTick; + for (int i=lowTick+1; i<=highTick; ++i) + { + subTickStep = (mTickVector.at(i)-mTickVector.at(i-1))/(double)(mSubTickCount+1); + for (int k=1; k<=mSubTickCount; ++k) + { + subTickPosition = mTickVector.at(i-1) + k*subTickStep; + if (subTickPosition < mRange.lower) + continue; + if (subTickPosition > mRange.upper) + { + done = true; + break; + } + mSubTickVector[subTickIndex] = subTickPosition; + subTickIndex++; + } + if (done) break; + } + mSubTickVector.resize(subTickIndex); + } + + // generate tick labels according to tick positions: + if (mAutoTickLabels) + { + int vecsize = mTickVector.size(); + mTickVectorLabels.resize(vecsize); + if (mTickLabelType == ltNumber) + { + for (int i=mLowestVisibleTick; i<=mHighestVisibleTick; ++i) + mTickVectorLabels[i] = mParentPlot->locale().toString(mTickVector.at(i), mNumberFormatChar.toLatin1(), mNumberPrecision); + } else if (mTickLabelType == ltDateTime) + { + for (int i=mLowestVisibleTick; i<=mHighestVisibleTick; ++i) + { +#if QT_VERSION < QT_VERSION_CHECK(4, 7, 0) // use fromMSecsSinceEpoch function if available, to gain sub-second accuracy on tick labels (e.g. for format "hh:mm:ss:zzz") + mTickVectorLabels[i] = mParentPlot->locale().toString(QDateTime::fromTime_t(mTickVector.at(i)).toTimeSpec(mDateTimeSpec), mDateTimeFormat); +#else + mTickVectorLabels[i] = mParentPlot->locale().toString(QDateTime::fromMSecsSinceEpoch(mTickVector.at(i)*1000).toTimeSpec(mDateTimeSpec), mDateTimeFormat); +#endif + } + } + } else // mAutoTickLabels == false + { + if (mAutoTicks) // ticks generated automatically, but not ticklabels, so emit ticksRequest here for labels + { + emit ticksRequest(); + } + // make sure provided tick label vector has correct (minimal) length: + if (mTickVectorLabels.size() < mTickVector.size()) + mTickVectorLabels.resize(mTickVector.size()); + } +} + +/*! \internal + + If \ref setAutoTicks is set to true, this function is called by \ref setupTickVectors to + generate reasonable tick positions (and subtick count). The algorithm tries to create + approximately mAutoTickCount ticks (set via \ref setAutoTickCount). + + If the scale is logarithmic, \ref setAutoTickCount is ignored, and one tick is generated at every + power of the current logarithm base, set via \ref setScaleLogBase. +*/ +void QCPAxis::generateAutoTicks() +{ + if (mScaleType == stLinear) + { + if (mAutoTickStep) + { + // Generate tick positions according to linear scaling: + mTickStep = mRange.size()/(double)(mAutoTickCount+1e-10); // mAutoTickCount ticks on average, the small addition is to prevent jitter on exact integers + double magnitudeFactor = qPow(10.0, qFloor(qLn(mTickStep)/qLn(10.0))); // get magnitude factor e.g. 0.01, 1, 10, 1000 etc. + double tickStepMantissa = mTickStep/magnitudeFactor; + if (tickStepMantissa < 5) + { + // round digit after decimal point to 0.5 + mTickStep = (int)(tickStepMantissa*2)/2.0*magnitudeFactor; + } else + { + // round to first digit in multiples of 2 + mTickStep = (int)(tickStepMantissa/2.0)*2.0*magnitudeFactor; + } + } + if (mAutoSubTicks) + mSubTickCount = calculateAutoSubTickCount(mTickStep); + // Generate tick positions according to mTickStep: + qint64 firstStep = floor(mRange.lower/mTickStep); // do not use qFloor here, or we'll lose 64 bit precision + qint64 lastStep = ceil(mRange.upper/mTickStep); // do not use qCeil here, or we'll lose 64 bit precision + int tickcount = lastStep-firstStep+1; + if (tickcount < 0) tickcount = 0; + mTickVector.resize(tickcount); + for (int i=0; i 0 && mRange.upper > 0) // positive range + { + double lowerMag = basePow(qFloor(baseLog(mRange.lower))); + double currentMag = lowerMag; + mTickVector.clear(); + mTickVector.append(currentMag); + while (currentMag < mRange.upper && currentMag > 0) // currentMag might be zero for ranges ~1e-300, just cancel in that case + { + currentMag *= mScaleLogBase; + mTickVector.append(currentMag); + } + } else if (mRange.lower < 0 && mRange.upper < 0) // negative range + { + double lowerMag = -basePow(qCeil(baseLog(-mRange.lower))); + double currentMag = lowerMag; + mTickVector.clear(); + mTickVector.append(currentMag); + while (currentMag < mRange.upper && currentMag < 0) // currentMag might be zero for ranges ~1e-300, just cancel in that case + { + currentMag /= mScaleLogBase; + mTickVector.append(currentMag); + } + } else // invalid range for logarithmic scale, because lower and upper have different sign + { + mTickVector.clear(); + qDebug() << Q_FUNC_INFO << "Invalid range for logarithmic plot: " << mRange.lower << "-" << mRange.upper; + } + } +} + +/*! \internal + + Called by generateAutoTicks when \ref setAutoSubTicks is set to true. Depending on the \a + tickStep between two major ticks on the axis, a different number of sub ticks is appropriate. For + Example taking 4 sub ticks for a \a tickStep of 1 makes more sense than taking 5 sub ticks, + because this corresponds to a sub tick step of 0.2, instead of the less intuitive 0.16667. Note + that a subtick count of 4 means dividing the major tick step into 5 sections. + + This is implemented by a hand made lookup for integer tick steps as well as fractional tick steps + with a fractional part of (approximately) 0.5. If a tick step is different (i.e. has no + fractional part close to 0.5), the currently set sub tick count (\ref setSubTickCount) is + returned. +*/ +int QCPAxis::calculateAutoSubTickCount(double tickStep) const +{ + int result = mSubTickCount; // default to current setting, if no proper value can be found + + // get mantissa of tickstep: + double magnitudeFactor = qPow(10.0, qFloor(qLn(tickStep)/qLn(10.0))); // get magnitude factor e.g. 0.01, 1, 10, 1000 etc. + double tickStepMantissa = tickStep/magnitudeFactor; + + // separate integer and fractional part of mantissa: + double epsilon = 0.01; + double intPartf; + int intPart; + double fracPart = modf(tickStepMantissa, &intPartf); + intPart = intPartf; + + // handle cases with (almost) integer mantissa: + if (fracPart < epsilon || 1.0-fracPart < epsilon) + { + if (1.0-fracPart < epsilon) + ++intPart; + switch (intPart) + { + case 1: result = 4; break; // 1.0 -> 0.2 substep + case 2: result = 3; break; // 2.0 -> 0.5 substep + case 3: result = 2; break; // 3.0 -> 1.0 substep + case 4: result = 3; break; // 4.0 -> 1.0 substep + case 5: result = 4; break; // 5.0 -> 1.0 substep + case 6: result = 2; break; // 6.0 -> 2.0 substep + case 7: result = 6; break; // 7.0 -> 1.0 substep + case 8: result = 3; break; // 8.0 -> 2.0 substep + case 9: result = 2; break; // 9.0 -> 3.0 substep + } + } else + { + // handle cases with significantly fractional mantissa: + if (qAbs(fracPart-0.5) < epsilon) // *.5 mantissa + { + switch (intPart) + { + case 1: result = 2; break; // 1.5 -> 0.5 substep + case 2: result = 4; break; // 2.5 -> 0.5 substep + case 3: result = 4; break; // 3.5 -> 0.7 substep + case 4: result = 2; break; // 4.5 -> 1.5 substep + case 5: result = 4; break; // 5.5 -> 1.1 substep (won't occur with autoTickStep from here on) + case 6: result = 4; break; // 6.5 -> 1.3 substep + case 7: result = 2; break; // 7.5 -> 2.5 substep + case 8: result = 4; break; // 8.5 -> 1.7 substep + case 9: result = 4; break; // 9.5 -> 1.9 substep + } + } + // if mantissa fraction isnt 0.0 or 0.5, don't bother finding good sub tick marks, leave default + } + + return result; +} + +/* inherits documentation from base class */ +void QCPAxis::selectEvent(QMouseEvent *event, bool additive, const QVariant &details, bool *selectionStateChanged) +{ + Q_UNUSED(event) + SelectablePart part = details.value(); + if (mSelectableParts.testFlag(part)) + { + SelectableParts selBefore = mSelectedParts; + setSelectedParts(additive ? mSelectedParts^part : part); + if (selectionStateChanged) + *selectionStateChanged = mSelectedParts != selBefore; + } +} + +/* inherits documentation from base class */ +void QCPAxis::deselectEvent(bool *selectionStateChanged) +{ + SelectableParts selBefore = mSelectedParts; + setSelectedParts(mSelectedParts & ~mSelectableParts); + if (selectionStateChanged) + *selectionStateChanged = mSelectedParts != selBefore; +} + +/*! \internal + + A convenience function to easily set the QPainter::Antialiased hint on the provided \a painter + before drawing axis lines. + + This is the antialiasing state the painter passed to the \ref draw method is in by default. + + This function takes into account the local setting of the antialiasing flag as well as the + overrides set with \ref QCustomPlot::setAntialiasedElements and \ref + QCustomPlot::setNotAntialiasedElements. + + \see setAntialiased +*/ +void QCPAxis::applyDefaultAntialiasingHint(QCPPainter *painter) const +{ + applyAntialiasingHint(painter, mAntialiased, QCP::aeAxes); +} + +/*! \internal + + Draws the axis with the specified \a painter, using the internal QCPAxisPainterPrivate instance. + +*/ +void QCPAxis::draw(QCPPainter *painter) +{ + const int lowTick = mLowestVisibleTick; + const int highTick = mHighestVisibleTick; + QVector subTickPositions; // the final coordToPixel transformed vector passed to QCPAxisPainter + QVector tickPositions; // the final coordToPixel transformed vector passed to QCPAxisPainter + QVector tickLabels; // the final vector passed to QCPAxisPainter + tickPositions.reserve(highTick-lowTick+1); + tickLabels.reserve(highTick-lowTick+1); + subTickPositions.reserve(mSubTickVector.size()); + + if (mTicks) + { + for (int i=lowTick; i<=highTick; ++i) + { + tickPositions.append(coordToPixel(mTickVector.at(i))); + if (mTickLabels) + tickLabels.append(mTickVectorLabels.at(i)); + } + + if (mSubTickCount > 0) + { + const int subTickCount = mSubTickVector.size(); + for (int i=0; itype = mAxisType; + mAxisPainter->basePen = getBasePen(); + mAxisPainter->labelFont = getLabelFont(); + mAxisPainter->labelColor = getLabelColor(); + mAxisPainter->label = mLabel; + mAxisPainter->substituteExponent = mAutoTickLabels && mNumberBeautifulPowers && mTickLabelType == ltNumber; + mAxisPainter->tickPen = getTickPen(); + mAxisPainter->subTickPen = getSubTickPen(); + mAxisPainter->tickLabelFont = getTickLabelFont(); + mAxisPainter->tickLabelColor = getTickLabelColor(); + mAxisPainter->axisRect = mAxisRect->rect(); + mAxisPainter->viewportRect = mParentPlot->viewport(); + mAxisPainter->abbreviateDecimalPowers = mScaleType == stLogarithmic; + mAxisPainter->reversedEndings = mRangeReversed; + mAxisPainter->tickPositions = tickPositions; + mAxisPainter->tickLabels = tickLabels; + mAxisPainter->subTickPositions = subTickPositions; + mAxisPainter->draw(painter); +} + +/*! \internal + + Returns via \a lowIndex and \a highIndex, which ticks in the current tick vector are visible in + the current range. The return values are indices of the tick vector, not the positions of the + ticks themselves. + + The actual use of this function is when an external tick vector is provided, since it might + exceed far beyond the currently displayed range, and would cause unnecessary calculations e.g. of + subticks. + + If all ticks are outside the axis range, an inverted range is returned, i.e. highIndex will be + smaller than lowIndex. There is one case, where this function returns indices that are not really + visible in the current axis range: When the tick spacing is larger than the axis range size and + one tick is below the axis range and the next tick is already above the axis range. Because in + such cases it is usually desirable to know the tick pair, to draw proper subticks. +*/ +void QCPAxis::visibleTickBounds(int &lowIndex, int &highIndex) const +{ + bool lowFound = false; + bool highFound = false; + lowIndex = 0; + highIndex = -1; + + for (int i=0; i < mTickVector.size(); ++i) + { + if (mTickVector.at(i) >= mRange.lower) + { + lowFound = true; + lowIndex = i; + break; + } + } + for (int i=mTickVector.size()-1; i >= 0; --i) + { + if (mTickVector.at(i) <= mRange.upper) + { + highFound = true; + highIndex = i; + break; + } + } + + if (!lowFound && highFound) + lowIndex = highIndex+1; + else if (lowFound && !highFound) + highIndex = lowIndex-1; +} + +/*! \internal + + A log function with the base mScaleLogBase, used mostly for coordinate transforms in logarithmic + scales with arbitrary log base. Uses the buffered mScaleLogBaseLogInv for faster calculation. + This is set to 1.0/qLn(mScaleLogBase) in \ref setScaleLogBase. + + \see basePow, setScaleLogBase, setScaleType +*/ +double QCPAxis::baseLog(double value) const +{ + return qLn(value)*mScaleLogBaseLogInv; +} + +/*! \internal + + A power function with the base mScaleLogBase, used mostly for coordinate transforms in + logarithmic scales with arbitrary log base. + + \see baseLog, setScaleLogBase, setScaleType +*/ +double QCPAxis::basePow(double value) const +{ + return qPow(mScaleLogBase, value); +} + +/*! \internal + + Returns the pen that is used to draw the axis base line. Depending on the selection state, this + is either mSelectedBasePen or mBasePen. +*/ +QPen QCPAxis::getBasePen() const +{ + return mSelectedParts.testFlag(spAxis) ? mSelectedBasePen : mBasePen; +} + +/*! \internal + + Returns the pen that is used to draw the (major) ticks. Depending on the selection state, this + is either mSelectedTickPen or mTickPen. +*/ +QPen QCPAxis::getTickPen() const +{ + return mSelectedParts.testFlag(spAxis) ? mSelectedTickPen : mTickPen; +} + +/*! \internal + + Returns the pen that is used to draw the subticks. Depending on the selection state, this + is either mSelectedSubTickPen or mSubTickPen. +*/ +QPen QCPAxis::getSubTickPen() const +{ + return mSelectedParts.testFlag(spAxis) ? mSelectedSubTickPen : mSubTickPen; +} + +/*! \internal + + Returns the font that is used to draw the tick labels. Depending on the selection state, this + is either mSelectedTickLabelFont or mTickLabelFont. +*/ +QFont QCPAxis::getTickLabelFont() const +{ + return mSelectedParts.testFlag(spTickLabels) ? mSelectedTickLabelFont : mTickLabelFont; +} + +/*! \internal + + Returns the font that is used to draw the axis label. Depending on the selection state, this + is either mSelectedLabelFont or mLabelFont. +*/ +QFont QCPAxis::getLabelFont() const +{ + return mSelectedParts.testFlag(spAxisLabel) ? mSelectedLabelFont : mLabelFont; +} + +/*! \internal + + Returns the color that is used to draw the tick labels. Depending on the selection state, this + is either mSelectedTickLabelColor or mTickLabelColor. +*/ +QColor QCPAxis::getTickLabelColor() const +{ + return mSelectedParts.testFlag(spTickLabels) ? mSelectedTickLabelColor : mTickLabelColor; +} + +/*! \internal + + Returns the color that is used to draw the axis label. Depending on the selection state, this + is either mSelectedLabelColor or mLabelColor. +*/ +QColor QCPAxis::getLabelColor() const +{ + return mSelectedParts.testFlag(spAxisLabel) ? mSelectedLabelColor : mLabelColor; +} + +/*! \internal + + Returns the appropriate outward margin for this axis. It is needed if \ref + QCPAxisRect::setAutoMargins is set to true on the parent axis rect. An axis with axis type \ref + atLeft will return an appropriate left margin, \ref atBottom will return an appropriate bottom + margin and so forth. For the calculation, this function goes through similar steps as \ref draw, + so changing one function likely requires the modification of the other one as well. + + The margin consists of the outward tick length, tick label padding, tick label size, label + padding, label size, and padding. + + The margin is cached internally, so repeated calls while leaving the axis range, fonts, etc. + unchanged are very fast. +*/ +int QCPAxis::calculateMargin() +{ + if (!mVisible) // if not visible, directly return 0, don't cache 0 because we can't react to setVisible in QCPAxis + return 0; + + if (mCachedMarginValid) + return mCachedMargin; + + // run through similar steps as QCPAxis::draw, and caluclate margin needed to fit axis and its labels + int margin = 0; + + int lowTick, highTick; + visibleTickBounds(lowTick, highTick); + QVector tickPositions; // the final coordToPixel transformed vector passed to QCPAxisPainter + QVector tickLabels; // the final vector passed to QCPAxisPainter + tickPositions.reserve(highTick-lowTick+1); + tickLabels.reserve(highTick-lowTick+1); + if (mTicks) + { + for (int i=lowTick; i<=highTick; ++i) + { + tickPositions.append(coordToPixel(mTickVector.at(i))); + if (mTickLabels) + tickLabels.append(mTickVectorLabels.at(i)); + } + } + // transfer all properties of this axis to QCPAxisPainterPrivate which it needs to calculate the size. + // Note that some axis painter properties are already set by direct feed-through with QCPAxis setters + mAxisPainter->type = mAxisType; + mAxisPainter->labelFont = getLabelFont(); + mAxisPainter->label = mLabel; + mAxisPainter->tickLabelFont = mTickLabelFont; + mAxisPainter->axisRect = mAxisRect->rect(); + mAxisPainter->viewportRect = mParentPlot->viewport(); + mAxisPainter->tickPositions = tickPositions; + mAxisPainter->tickLabels = tickLabels; + margin += mAxisPainter->size(); + margin += mPadding; + + mCachedMargin = margin; + mCachedMarginValid = true; + return margin; +} + +/* inherits documentation from base class */ +QCP::Interaction QCPAxis::selectionCategory() const +{ + return QCP::iSelectAxes; +} + + +//////////////////////////////////////////////////////////////////////////////////////////////////// +//////////////////// QCPAxisPainterPrivate +//////////////////////////////////////////////////////////////////////////////////////////////////// + +/*! \class QCPAxisPainterPrivate + + \internal + \brief (Private) + + This is a private class and not part of the public QCustomPlot interface. + + It is used by QCPAxis to do the low-level drawing of axis backbone, tick marks, tick labels and + axis label. It also buffers the labels to reduce replot times. The parameters are configured by + directly accessing the public member variables. +*/ + +/*! + Constructs a QCPAxisPainterPrivate instance. Make sure to not create a new instance on every + redraw, to utilize the caching mechanisms. +*/ +QCPAxisPainterPrivate::QCPAxisPainterPrivate(QCustomPlot *parentPlot) : + type(QCPAxis::atLeft), + basePen(QPen(Qt::black, 0, Qt::SolidLine, Qt::SquareCap)), + lowerEnding(QCPLineEnding::esNone), + upperEnding(QCPLineEnding::esNone), + labelPadding(0), + tickLabelPadding(0), + tickLabelRotation(0), + tickLabelSide(QCPAxis::lsOutside), + substituteExponent(true), + numberMultiplyCross(false), + tickLengthIn(5), + tickLengthOut(0), + subTickLengthIn(2), + subTickLengthOut(0), + tickPen(QPen(Qt::black, 0, Qt::SolidLine, Qt::SquareCap)), + subTickPen(QPen(Qt::black, 0, Qt::SolidLine, Qt::SquareCap)), + offset(0), + abbreviateDecimalPowers(false), + reversedEndings(false), + mParentPlot(parentPlot), + mLabelCache(16) // cache at most 16 (tick) labels +{ +} + +QCPAxisPainterPrivate::~QCPAxisPainterPrivate() +{ +} + +/*! \internal + + Draws the axis with the specified \a painter. + + The selection boxes (mAxisSelectionBox, mTickLabelsSelectionBox, mLabelSelectionBox) are set + here, too. +*/ +void QCPAxisPainterPrivate::draw(QCPPainter *painter) +{ + QByteArray newHash = generateLabelParameterHash(); + if (newHash != mLabelParameterHash) + { + mLabelCache.clear(); + mLabelParameterHash = newHash; + } + + QPoint origin; + switch (type) + { + case QCPAxis::atLeft: origin = axisRect.bottomLeft() +QPoint(-offset, 0); break; + case QCPAxis::atRight: origin = axisRect.bottomRight()+QPoint(+offset, 0); break; + case QCPAxis::atTop: origin = axisRect.topLeft() +QPoint(0, -offset); break; + case QCPAxis::atBottom: origin = axisRect.bottomLeft() +QPoint(0, +offset); break; + } + + double xCor = 0, yCor = 0; // paint system correction, for pixel exact matches (affects baselines and ticks of top/right axes) + switch (type) + { + case QCPAxis::atTop: yCor = -1; break; + case QCPAxis::atRight: xCor = 1; break; + default: break; + } + int margin = 0; + // draw baseline: + QLineF baseLine; + painter->setPen(basePen); + if (QCPAxis::orientation(type) == Qt::Horizontal) + baseLine.setPoints(origin+QPointF(xCor, yCor), origin+QPointF(axisRect.width()+xCor, yCor)); + else + baseLine.setPoints(origin+QPointF(xCor, yCor), origin+QPointF(xCor, -axisRect.height()+yCor)); + if (reversedEndings) + baseLine = QLineF(baseLine.p2(), baseLine.p1()); // won't make a difference for line itself, but for line endings later + painter->drawLine(baseLine); + + // draw ticks: + if (!tickPositions.isEmpty()) + { + painter->setPen(tickPen); + int tickDir = (type == QCPAxis::atBottom || type == QCPAxis::atRight) ? -1 : 1; // direction of ticks ("inward" is right for left axis and left for right axis) + if (QCPAxis::orientation(type) == Qt::Horizontal) + { + for (int i=0; idrawLine(QLineF(tickPositions.at(i)+xCor, origin.y()-tickLengthOut*tickDir+yCor, tickPositions.at(i)+xCor, origin.y()+tickLengthIn*tickDir+yCor)); + } else + { + for (int i=0; idrawLine(QLineF(origin.x()-tickLengthOut*tickDir+xCor, tickPositions.at(i)+yCor, origin.x()+tickLengthIn*tickDir+xCor, tickPositions.at(i)+yCor)); + } + } + + // draw subticks: + if (!subTickPositions.isEmpty()) + { + painter->setPen(subTickPen); + // direction of ticks ("inward" is right for left axis and left for right axis) + int tickDir = (type == QCPAxis::atBottom || type == QCPAxis::atRight) ? -1 : 1; + if (QCPAxis::orientation(type) == Qt::Horizontal) + { + for (int i=0; idrawLine(QLineF(subTickPositions.at(i)+xCor, origin.y()-subTickLengthOut*tickDir+yCor, subTickPositions.at(i)+xCor, origin.y()+subTickLengthIn*tickDir+yCor)); + } else + { + for (int i=0; idrawLine(QLineF(origin.x()-subTickLengthOut*tickDir+xCor, subTickPositions.at(i)+yCor, origin.x()+subTickLengthIn*tickDir+xCor, subTickPositions.at(i)+yCor)); + } + } + margin += qMax(0, qMax(tickLengthOut, subTickLengthOut)); + + // draw axis base endings: + bool antialiasingBackup = painter->antialiasing(); + painter->setAntialiasing(true); // always want endings to be antialiased, even if base and ticks themselves aren't + painter->setBrush(QBrush(basePen.color())); + QVector2D baseLineVector(baseLine.dx(), baseLine.dy()); + if (lowerEnding.style() != QCPLineEnding::esNone) + lowerEnding.draw(painter, QVector2D(baseLine.p1())-baseLineVector.normalized()*lowerEnding.realLength()*(lowerEnding.inverted()?-1:1), -baseLineVector); + if (upperEnding.style() != QCPLineEnding::esNone) + upperEnding.draw(painter, QVector2D(baseLine.p2())+baseLineVector.normalized()*upperEnding.realLength()*(upperEnding.inverted()?-1:1), baseLineVector); + painter->setAntialiasing(antialiasingBackup); + + // tick labels: + QRect oldClipRect; + if (tickLabelSide == QCPAxis::lsInside) // if using inside labels, clip them to the axis rect + { + oldClipRect = painter->clipRegion().boundingRect(); + painter->setClipRect(axisRect); + } + QSize tickLabelsSize(0, 0); // size of largest tick label, for offset calculation of axis label + if (!tickLabels.isEmpty()) + { + if (tickLabelSide == QCPAxis::lsOutside) + margin += tickLabelPadding; + painter->setFont(tickLabelFont); + painter->setPen(QPen(tickLabelColor)); + const int maxLabelIndex = qMin(tickPositions.size(), tickLabels.size()); + int distanceToAxis = margin; + if (tickLabelSide == QCPAxis::lsInside) + distanceToAxis = -(qMax(tickLengthIn, subTickLengthIn)+tickLabelPadding); + for (int i=0; isetClipRect(oldClipRect); + + // axis label: + QRect labelBounds; + if (!label.isEmpty()) + { + margin += labelPadding; + painter->setFont(labelFont); + painter->setPen(QPen(labelColor)); + labelBounds = painter->fontMetrics().boundingRect(0, 0, 0, 0, Qt::TextDontClip, label); + if (type == QCPAxis::atLeft) + { + QTransform oldTransform = painter->transform(); + painter->translate((origin.x()-margin-labelBounds.height()), origin.y()); + painter->rotate(-90); + painter->drawText(0, 0, axisRect.height(), labelBounds.height(), Qt::TextDontClip | Qt::AlignCenter, label); + painter->setTransform(oldTransform); + } + else if (type == QCPAxis::atRight) + { + QTransform oldTransform = painter->transform(); + painter->translate((origin.x()+margin+labelBounds.height()), origin.y()-axisRect.height()); + painter->rotate(90); + painter->drawText(0, 0, axisRect.height(), labelBounds.height(), Qt::TextDontClip | Qt::AlignCenter, label); + painter->setTransform(oldTransform); + } + else if (type == QCPAxis::atTop) + painter->drawText(origin.x(), origin.y()-margin-labelBounds.height(), axisRect.width(), labelBounds.height(), Qt::TextDontClip | Qt::AlignCenter, label); + else if (type == QCPAxis::atBottom) + painter->drawText(origin.x(), origin.y()+margin, axisRect.width(), labelBounds.height(), Qt::TextDontClip | Qt::AlignCenter, label); + } + + // set selection boxes: + int selectionTolerance = 0; + if (mParentPlot) + selectionTolerance = mParentPlot->selectionTolerance(); + else + qDebug() << Q_FUNC_INFO << "mParentPlot is null"; + int selAxisOutSize = qMax(qMax(tickLengthOut, subTickLengthOut), selectionTolerance); + int selAxisInSize = selectionTolerance; + int selTickLabelSize; + int selTickLabelOffset; + if (tickLabelSide == QCPAxis::lsOutside) + { + selTickLabelSize = (QCPAxis::orientation(type) == Qt::Horizontal ? tickLabelsSize.height() : tickLabelsSize.width()); + selTickLabelOffset = qMax(tickLengthOut, subTickLengthOut)+tickLabelPadding; + } else + { + selTickLabelSize = -(QCPAxis::orientation(type) == Qt::Horizontal ? tickLabelsSize.height() : tickLabelsSize.width()); + selTickLabelOffset = -(qMax(tickLengthIn, subTickLengthIn)+tickLabelPadding); + } + int selLabelSize = labelBounds.height(); + int selLabelOffset = qMax(tickLengthOut, subTickLengthOut)+(!tickLabels.isEmpty() && tickLabelSide == QCPAxis::lsOutside ? tickLabelPadding+selTickLabelSize : 0)+labelPadding; + if (type == QCPAxis::atLeft) + { + mAxisSelectionBox.setCoords(origin.x()-selAxisOutSize, axisRect.top(), origin.x()+selAxisInSize, axisRect.bottom()); + mTickLabelsSelectionBox.setCoords(origin.x()-selTickLabelOffset-selTickLabelSize, axisRect.top(), origin.x()-selTickLabelOffset, axisRect.bottom()); + mLabelSelectionBox.setCoords(origin.x()-selLabelOffset-selLabelSize, axisRect.top(), origin.x()-selLabelOffset, axisRect.bottom()); + } else if (type == QCPAxis::atRight) + { + mAxisSelectionBox.setCoords(origin.x()-selAxisInSize, axisRect.top(), origin.x()+selAxisOutSize, axisRect.bottom()); + mTickLabelsSelectionBox.setCoords(origin.x()+selTickLabelOffset+selTickLabelSize, axisRect.top(), origin.x()+selTickLabelOffset, axisRect.bottom()); + mLabelSelectionBox.setCoords(origin.x()+selLabelOffset+selLabelSize, axisRect.top(), origin.x()+selLabelOffset, axisRect.bottom()); + } else if (type == QCPAxis::atTop) + { + mAxisSelectionBox.setCoords(axisRect.left(), origin.y()-selAxisOutSize, axisRect.right(), origin.y()+selAxisInSize); + mTickLabelsSelectionBox.setCoords(axisRect.left(), origin.y()-selTickLabelOffset-selTickLabelSize, axisRect.right(), origin.y()-selTickLabelOffset); + mLabelSelectionBox.setCoords(axisRect.left(), origin.y()-selLabelOffset-selLabelSize, axisRect.right(), origin.y()-selLabelOffset); + } else if (type == QCPAxis::atBottom) + { + mAxisSelectionBox.setCoords(axisRect.left(), origin.y()-selAxisInSize, axisRect.right(), origin.y()+selAxisOutSize); + mTickLabelsSelectionBox.setCoords(axisRect.left(), origin.y()+selTickLabelOffset+selTickLabelSize, axisRect.right(), origin.y()+selTickLabelOffset); + mLabelSelectionBox.setCoords(axisRect.left(), origin.y()+selLabelOffset+selLabelSize, axisRect.right(), origin.y()+selLabelOffset); + } + mAxisSelectionBox = mAxisSelectionBox.normalized(); + mTickLabelsSelectionBox = mTickLabelsSelectionBox.normalized(); + mLabelSelectionBox = mLabelSelectionBox.normalized(); + // draw hitboxes for debug purposes: + //painter->setBrush(Qt::NoBrush); + //painter->drawRects(QVector() << mAxisSelectionBox << mTickLabelsSelectionBox << mLabelSelectionBox); +} + +/*! \internal + + Returns the size ("margin" in QCPAxisRect context, so measured perpendicular to the axis backbone + direction) needed to fit the axis. +*/ +int QCPAxisPainterPrivate::size() const +{ + int result = 0; + + // get length of tick marks pointing outwards: + if (!tickPositions.isEmpty()) + result += qMax(0, qMax(tickLengthOut, subTickLengthOut)); + + // calculate size of tick labels: + if (tickLabelSide == QCPAxis::lsOutside) + { + QSize tickLabelsSize(0, 0); + if (!tickLabels.isEmpty()) + { + for (int i=0; iplottingHints().testFlag(QCP::phCacheLabels) && !painter->modes().testFlag(QCPPainter::pmNoCaching)) // label caching enabled + { + CachedLabel *cachedLabel = mLabelCache.take(text); // attempt to get label from cache + if (!cachedLabel) // no cached label existed, create it + { + cachedLabel = new CachedLabel; + TickLabelData labelData = getTickLabelData(painter->font(), text); + cachedLabel->offset = getTickLabelDrawOffset(labelData)+labelData.rotatedTotalBounds.topLeft(); + cachedLabel->pixmap = QPixmap(labelData.rotatedTotalBounds.size()); + cachedLabel->pixmap.fill(Qt::transparent); + QCPPainter cachePainter(&cachedLabel->pixmap); + cachePainter.setPen(painter->pen()); + drawTickLabel(&cachePainter, -labelData.rotatedTotalBounds.topLeft().x(), -labelData.rotatedTotalBounds.topLeft().y(), labelData); + } + // if label would be partly clipped by widget border on sides, don't draw it (only for outside tick labels): + bool labelClippedByBorder = false; + if (tickLabelSide == QCPAxis::lsOutside) + { + if (QCPAxis::orientation(type) == Qt::Horizontal) + labelClippedByBorder = labelAnchor.x()+cachedLabel->offset.x()+cachedLabel->pixmap.width() > viewportRect.right() || labelAnchor.x()+cachedLabel->offset.x() < viewportRect.left(); + else + labelClippedByBorder = labelAnchor.y()+cachedLabel->offset.y()+cachedLabel->pixmap.height() > viewportRect.bottom() || labelAnchor.y()+cachedLabel->offset.y() < viewportRect.top(); + } + if (!labelClippedByBorder) + { + painter->drawPixmap(labelAnchor+cachedLabel->offset, cachedLabel->pixmap); + finalSize = cachedLabel->pixmap.size(); + } + mLabelCache.insert(text, cachedLabel); // return label to cache or insert for the first time if newly created + } else // label caching disabled, draw text directly on surface: + { + TickLabelData labelData = getTickLabelData(painter->font(), text); + QPointF finalPosition = labelAnchor + getTickLabelDrawOffset(labelData); + // if label would be partly clipped by widget border on sides, don't draw it (only for outside tick labels): + bool labelClippedByBorder = false; + if (tickLabelSide == QCPAxis::lsOutside) + { + if (QCPAxis::orientation(type) == Qt::Horizontal) + labelClippedByBorder = finalPosition.x()+(labelData.rotatedTotalBounds.width()+labelData.rotatedTotalBounds.left()) > viewportRect.right() || finalPosition.x()+labelData.rotatedTotalBounds.left() < viewportRect.left(); + else + labelClippedByBorder = finalPosition.y()+(labelData.rotatedTotalBounds.height()+labelData.rotatedTotalBounds.top()) > viewportRect.bottom() || finalPosition.y()+labelData.rotatedTotalBounds.top() < viewportRect.top(); + } + if (!labelClippedByBorder) + { + drawTickLabel(painter, finalPosition.x(), finalPosition.y(), labelData); + finalSize = labelData.rotatedTotalBounds.size(); + } + } + + // expand passed tickLabelsSize if current tick label is larger: + if (finalSize.width() > tickLabelsSize->width()) + tickLabelsSize->setWidth(finalSize.width()); + if (finalSize.height() > tickLabelsSize->height()) + tickLabelsSize->setHeight(finalSize.height()); +} + +/*! \internal + + This is a \ref placeTickLabel helper function. + + Draws the tick label specified in \a labelData with \a painter at the pixel positions \a x and \a + y. This function is used by \ref placeTickLabel to create new tick labels for the cache, or to + directly draw the labels on the QCustomPlot surface when label caching is disabled, i.e. when + QCP::phCacheLabels plotting hint is not set. +*/ +void QCPAxisPainterPrivate::drawTickLabel(QCPPainter *painter, double x, double y, const TickLabelData &labelData) const +{ + // backup painter settings that we're about to change: + QTransform oldTransform = painter->transform(); + QFont oldFont = painter->font(); + + // transform painter to position/rotation: + painter->translate(x, y); + if (!qFuzzyIsNull(tickLabelRotation)) + painter->rotate(tickLabelRotation); + + // draw text: + if (!labelData.expPart.isEmpty()) // indicator that beautiful powers must be used + { + painter->setFont(labelData.baseFont); + painter->drawText(0, 0, 0, 0, Qt::TextDontClip, labelData.basePart); + painter->setFont(labelData.expFont); + painter->drawText(labelData.baseBounds.width()+1, 0, labelData.expBounds.width(), labelData.expBounds.height(), Qt::TextDontClip, labelData.expPart); + } else + { + painter->setFont(labelData.baseFont); + painter->drawText(0, 0, labelData.totalBounds.width(), labelData.totalBounds.height(), Qt::TextDontClip | Qt::AlignHCenter, labelData.basePart); + } + + // reset painter settings to what it was before: + painter->setTransform(oldTransform); + painter->setFont(oldFont); +} + +/*! \internal + + This is a \ref placeTickLabel helper function. + + Transforms the passed \a text and \a font to a tickLabelData structure that can then be further + processed by \ref getTickLabelDrawOffset and \ref drawTickLabel. It splits the text into base and + exponent if necessary (member substituteExponent) and calculates appropriate bounding boxes. +*/ +QCPAxisPainterPrivate::TickLabelData QCPAxisPainterPrivate::getTickLabelData(const QFont &font, const QString &text) const +{ + TickLabelData result; + + // determine whether beautiful decimal powers should be used + bool useBeautifulPowers = false; + int ePos = -1; // first index of exponent part, text before that will be basePart, text until eLast will be expPart + int eLast = -1; // last index of exponent part, rest of text after this will be suffixPart + if (substituteExponent) + { + ePos = text.indexOf(QLatin1Char('e')); + if (ePos > 0 && text.at(ePos-1).isDigit()) + { + eLast = ePos; + while (eLast+1 < text.size() && (text.at(eLast+1) == QLatin1Char('+') || text.at(eLast+1) == QLatin1Char('-') || text.at(eLast+1).isDigit())) + ++eLast; + if (eLast > ePos) // only if also to right of 'e' is a digit/+/- interpret it as beautifiable power + useBeautifulPowers = true; + } + } + + // calculate text bounding rects and do string preparation for beautiful decimal powers: + result.baseFont = font; + if (result.baseFont.pointSizeF() > 0) // might return -1 if specified with setPixelSize, in that case we can't do correction in next line + result.baseFont.setPointSizeF(result.baseFont.pointSizeF()+0.05); // QFontMetrics.boundingRect has a bug for exact point sizes that make the results oscillate due to internal rounding + if (useBeautifulPowers) + { + // split text into parts of number/symbol that will be drawn normally and part that will be drawn as exponent: + result.basePart = text.left(ePos); + // in log scaling, we want to turn "1*10^n" into "10^n", else add multiplication sign and decimal base: + if (abbreviateDecimalPowers && result.basePart == QLatin1String("1")) + result.basePart = QLatin1String("10"); + else + result.basePart += (numberMultiplyCross ? QString(QChar(215)) : QString(QChar(183))) + QLatin1String("10"); + result.expPart = text.mid(ePos+1); + // clip "+" and leading zeros off expPart: + while (result.expPart.length() > 2 && result.expPart.at(1) == QLatin1Char('0')) // length > 2 so we leave one zero when numberFormatChar is 'e' + result.expPart.remove(1, 1); + if (!result.expPart.isEmpty() && result.expPart.at(0) == QLatin1Char('+')) + result.expPart.remove(0, 1); + // prepare smaller font for exponent: + result.expFont = font; + if (result.expFont.pointSize() > 0) + result.expFont.setPointSize(result.expFont.pointSize()*0.75); + else + result.expFont.setPixelSize(result.expFont.pixelSize()*0.75); + // calculate bounding rects of base part, exponent part and total one: + result.baseBounds = QFontMetrics(result.baseFont).boundingRect(0, 0, 0, 0, Qt::TextDontClip, result.basePart); + result.expBounds = QFontMetrics(result.expFont).boundingRect(0, 0, 0, 0, Qt::TextDontClip, result.expPart); + result.totalBounds = result.baseBounds.adjusted(0, 0, result.expBounds.width()+2, 0); // +2 consists of the 1 pixel spacing between base and exponent (see drawTickLabel) and an extra pixel to include AA + } else // useBeautifulPowers == false + { + result.basePart = text; + result.totalBounds = QFontMetrics(result.baseFont).boundingRect(0, 0, 0, 0, Qt::TextDontClip | Qt::AlignHCenter, result.basePart); + } + result.totalBounds.moveTopLeft(QPoint(0, 0)); // want bounding box aligned top left at origin, independent of how it was created, to make further processing simpler + + // calculate possibly different bounding rect after rotation: + result.rotatedTotalBounds = result.totalBounds; + if (!qFuzzyIsNull(tickLabelRotation)) + { + QTransform transform; + transform.rotate(tickLabelRotation); + result.rotatedTotalBounds = transform.mapRect(result.rotatedTotalBounds); + } + + return result; +} + +/*! \internal + + This is a \ref placeTickLabel helper function. + + Calculates the offset at which the top left corner of the specified tick label shall be drawn. + The offset is relative to a point right next to the tick the label belongs to. + + This function is thus responsible for e.g. centering tick labels under ticks and positioning them + appropriately when they are rotated. +*/ +QPointF QCPAxisPainterPrivate::getTickLabelDrawOffset(const TickLabelData &labelData) const +{ + /* + calculate label offset from base point at tick (non-trivial, for best visual appearance): short + explanation for bottom axis: The anchor, i.e. the point in the label that is placed + horizontally under the corresponding tick is always on the label side that is closer to the + axis (e.g. the left side of the text when we're rotating clockwise). On that side, the height + is halved and the resulting point is defined the anchor. This way, a 90 degree rotated text + will be centered under the tick (i.e. displaced horizontally by half its height). At the same + time, a 45 degree rotated text will "point toward" its tick, as is typical for rotated tick + labels. + */ + bool doRotation = !qFuzzyIsNull(tickLabelRotation); + bool flip = qFuzzyCompare(qAbs(tickLabelRotation), 90.0); // perfect +/-90 degree flip. Indicates vertical label centering on vertical axes. + double radians = tickLabelRotation/180.0*M_PI; + int x=0, y=0; + if ((type == QCPAxis::atLeft && tickLabelSide == QCPAxis::lsOutside) || (type == QCPAxis::atRight && tickLabelSide == QCPAxis::lsInside)) // Anchor at right side of tick label + { + if (doRotation) + { + if (tickLabelRotation > 0) + { + x = -qCos(radians)*labelData.totalBounds.width(); + y = flip ? -labelData.totalBounds.width()/2.0 : -qSin(radians)*labelData.totalBounds.width()-qCos(radians)*labelData.totalBounds.height()/2.0; + } else + { + x = -qCos(-radians)*labelData.totalBounds.width()-qSin(-radians)*labelData.totalBounds.height(); + y = flip ? +labelData.totalBounds.width()/2.0 : +qSin(-radians)*labelData.totalBounds.width()-qCos(-radians)*labelData.totalBounds.height()/2.0; + } + } else + { + x = -labelData.totalBounds.width(); + y = -labelData.totalBounds.height()/2.0; + } + } else if ((type == QCPAxis::atRight && tickLabelSide == QCPAxis::lsOutside) || (type == QCPAxis::atLeft && tickLabelSide == QCPAxis::lsInside)) // Anchor at left side of tick label + { + if (doRotation) + { + if (tickLabelRotation > 0) + { + x = +qSin(radians)*labelData.totalBounds.height(); + y = flip ? -labelData.totalBounds.width()/2.0 : -qCos(radians)*labelData.totalBounds.height()/2.0; + } else + { + x = 0; + y = flip ? +labelData.totalBounds.width()/2.0 : -qCos(-radians)*labelData.totalBounds.height()/2.0; + } + } else + { + x = 0; + y = -labelData.totalBounds.height()/2.0; + } + } else if ((type == QCPAxis::atTop && tickLabelSide == QCPAxis::lsOutside) || (type == QCPAxis::atBottom && tickLabelSide == QCPAxis::lsInside)) // Anchor at bottom side of tick label + { + if (doRotation) + { + if (tickLabelRotation > 0) + { + x = -qCos(radians)*labelData.totalBounds.width()+qSin(radians)*labelData.totalBounds.height()/2.0; + y = -qSin(radians)*labelData.totalBounds.width()-qCos(radians)*labelData.totalBounds.height(); + } else + { + x = -qSin(-radians)*labelData.totalBounds.height()/2.0; + y = -qCos(-radians)*labelData.totalBounds.height(); + } + } else + { + x = -labelData.totalBounds.width()/2.0; + y = -labelData.totalBounds.height(); + } + } else if ((type == QCPAxis::atBottom && tickLabelSide == QCPAxis::lsOutside) || (type == QCPAxis::atTop && tickLabelSide == QCPAxis::lsInside)) // Anchor at top side of tick label + { + if (doRotation) + { + if (tickLabelRotation > 0) + { + x = +qSin(radians)*labelData.totalBounds.height()/2.0; + y = 0; + } else + { + x = -qCos(-radians)*labelData.totalBounds.width()-qSin(-radians)*labelData.totalBounds.height()/2.0; + y = +qSin(-radians)*labelData.totalBounds.width(); + } + } else + { + x = -labelData.totalBounds.width()/2.0; + y = 0; + } + } + + return QPointF(x, y); +} + +/*! \internal + + Simulates the steps done by \ref placeTickLabel by calculating bounding boxes of the text label + to be drawn, depending on number format etc. Since only the largest tick label is wanted for the + margin calculation, the passed \a tickLabelsSize is only expanded, if it's currently set to a + smaller width/height. +*/ +void QCPAxisPainterPrivate::getMaxTickLabelSize(const QFont &font, const QString &text, QSize *tickLabelsSize) const +{ + // note: this function must return the same tick label sizes as the placeTickLabel function. + QSize finalSize; + if (mParentPlot->plottingHints().testFlag(QCP::phCacheLabels) && mLabelCache.contains(text)) // label caching enabled and have cached label + { + const CachedLabel *cachedLabel = mLabelCache.object(text); + finalSize = cachedLabel->pixmap.size(); + } else // label caching disabled or no label with this text cached: + { + TickLabelData labelData = getTickLabelData(font, text); + finalSize = labelData.rotatedTotalBounds.size(); + } + + // expand passed tickLabelsSize if current tick label is larger: + if (finalSize.width() > tickLabelsSize->width()) + tickLabelsSize->setWidth(finalSize.width()); + if (finalSize.height() > tickLabelsSize->height()) + tickLabelsSize->setHeight(finalSize.height()); +} + + +//////////////////////////////////////////////////////////////////////////////////////////////////// +//////////////////// QCPAbstractPlottable +//////////////////////////////////////////////////////////////////////////////////////////////////// + +/*! \class QCPAbstractPlottable + \brief The abstract base class for all data representing objects in a plot. + + It defines a very basic interface like name, pen, brush, visibility etc. Since this class is + abstract, it can't be instantiated. Use one of the subclasses or create a subclass yourself to + create new ways of displaying data (see "Creating own plottables" below). + + All further specifics are in the subclasses, for example: + \li A normal graph with possibly a line, scatter points and error bars: \ref QCPGraph + (typically created with \ref QCustomPlot::addGraph) + \li A parametric curve: \ref QCPCurve + \li A bar chart: \ref QCPBars + \li A statistical box plot: \ref QCPStatisticalBox + \li A color encoded two-dimensional map: \ref QCPColorMap + \li An OHLC/Candlestick chart: \ref QCPFinancial + + \section plottables-subclassing Creating own plottables + + To create an own plottable, you implement a subclass of QCPAbstractPlottable. These are the pure + virtual functions, you must implement: + \li \ref clearData + \li \ref selectTest + \li \ref draw + \li \ref drawLegendIcon + \li \ref getKeyRange + \li \ref getValueRange + + See the documentation of those functions for what they need to do. + + For drawing your plot, you can use the \ref coordsToPixels functions to translate a point in plot + coordinates to pixel coordinates. This function is quite convenient, because it takes the + orientation of the key and value axes into account for you (x and y are swapped when the key axis + is vertical and the value axis horizontal). If you are worried about performance (i.e. you need + to translate many points in a loop like QCPGraph), you can directly use \ref + QCPAxis::coordToPixel. However, you must then take care about the orientation of the axis + yourself. + + Here are some important members you inherit from QCPAbstractPlottable: + + + + + + + + + + + + + + + + + + + + + + + + + + +
QCustomPlot *\b mParentPlotA pointer to the parent QCustomPlot instance. The parent plot is inferred from the axes that are passed in the constructor.
QString \b mNameThe name of the plottable.
QPen \b mPenThe generic pen of the plottable. You should use this pen for the most prominent data representing lines in the plottable (e.g QCPGraph uses this pen for its graph lines and scatters)
QPen \b mSelectedPenThe generic pen that should be used when the plottable is selected (hint: \ref mainPen gives you the right pen, depending on selection state).
QBrush \b mBrushThe generic brush of the plottable. You should use this brush for the most prominent fillable structures in the plottable (e.g. QCPGraph uses this brush to control filling under the graph)
QBrush \b mSelectedBrushThe generic brush that should be used when the plottable is selected (hint: \ref mainBrush gives you the right brush, depending on selection state).
QPointer\b mKeyAxis, \b mValueAxisThe key and value axes this plottable is attached to. Call their QCPAxis::coordToPixel functions to translate coordinates to pixels in either the key or value dimension. + Make sure to check whether the pointer is null before using it. If one of the axes is null, don't draw the plottable.
bool \b mSelectedindicates whether the plottable is selected or not.
+*/ + +/* start of documentation of pure virtual functions */ + +/*! \fn void QCPAbstractPlottable::clearData() = 0 + Clears all data in the plottable. +*/ + +/*! \fn void QCPAbstractPlottable::drawLegendIcon(QCPPainter *painter, const QRect &rect) const = 0 + \internal + + called by QCPLegend::draw (via QCPPlottableLegendItem::draw) to create a graphical representation + of this plottable inside \a rect, next to the plottable name. + + The passed \a painter has its cliprect set to \a rect, so painting outside of \a rect won't + appear outside the legend icon border. +*/ + +/*! \fn QCPRange QCPAbstractPlottable::getKeyRange(bool &foundRange, SignDomain inSignDomain) const = 0 + \internal + + called by rescaleAxes functions to get the full data key bounds. For logarithmic plots, one can + set \a inSignDomain to either \ref sdNegative or \ref sdPositive in order to restrict the + returned range to that sign domain. E.g. when only negative range is wanted, set \a inSignDomain + to \ref sdNegative and all positive points will be ignored for range calculation. For no + restriction, just set \a inSignDomain to \ref sdBoth (default). \a foundRange is an output + parameter that indicates whether a range could be found or not. If this is false, you shouldn't + use the returned range (e.g. no points in data). + + Note that \a foundRange is not the same as \ref QCPRange::validRange, since the range returned by + this function may have size zero, which wouldn't count as a valid range. + + \see rescaleAxes, getValueRange +*/ + +/*! \fn QCPRange QCPAbstractPlottable::getValueRange(bool &foundRange, SignDomain inSignDomain) const = 0 + \internal + + called by rescaleAxes functions to get the full data value bounds. For logarithmic plots, one can + set \a inSignDomain to either \ref sdNegative or \ref sdPositive in order to restrict the + returned range to that sign domain. E.g. when only negative range is wanted, set \a inSignDomain + to \ref sdNegative and all positive points will be ignored for range calculation. For no + restriction, just set \a inSignDomain to \ref sdBoth (default). \a foundRange is an output + parameter that indicates whether a range could be found or not. If this is false, you shouldn't + use the returned range (e.g. no points in data). + + Note that \a foundRange is not the same as \ref QCPRange::validRange, since the range returned by + this function may have size zero, which wouldn't count as a valid range. + + \see rescaleAxes, getKeyRange +*/ + +/* end of documentation of pure virtual functions */ +/* start of documentation of signals */ + +/*! \fn void QCPAbstractPlottable::selectionChanged(bool selected) + + This signal is emitted when the selection state of this plottable has changed, either by user + interaction or by a direct call to \ref setSelected. +*/ + +/*! \fn void QCPAbstractPlottable::selectableChanged(bool selectable); + + This signal is emitted when the selectability of this plottable has changed. + + \see setSelectable +*/ + +/* end of documentation of signals */ + +/*! + Constructs an abstract plottable which uses \a keyAxis as its key axis ("x") and \a valueAxis as + its value axis ("y"). \a keyAxis and \a valueAxis must reside in the same QCustomPlot instance + and have perpendicular orientations. If either of these restrictions is violated, a corresponding + message is printed to the debug output (qDebug), the construction is not aborted, though. + + Since QCPAbstractPlottable is an abstract class that defines the basic interface to plottables, + it can't be directly instantiated. + + You probably want one of the subclasses like \ref QCPGraph or \ref QCPCurve instead. +*/ +QCPAbstractPlottable::QCPAbstractPlottable(QCPAxis *keyAxis, QCPAxis *valueAxis) : + QCPLayerable(keyAxis->parentPlot(), QString(), keyAxis->axisRect()), + mName(), + mAntialiasedFill(true), + mAntialiasedScatters(true), + mAntialiasedErrorBars(false), + mPen(Qt::black), + mSelectedPen(Qt::black), + mBrush(Qt::NoBrush), + mSelectedBrush(Qt::NoBrush), + mKeyAxis(keyAxis), + mValueAxis(valueAxis), + mSelectable(true), + mSelected(false) +{ + if (keyAxis->parentPlot() != valueAxis->parentPlot()) + qDebug() << Q_FUNC_INFO << "Parent plot of keyAxis is not the same as that of valueAxis."; + if (keyAxis->orientation() == valueAxis->orientation()) + qDebug() << Q_FUNC_INFO << "keyAxis and valueAxis must be orthogonal to each other."; +} + +/*! + The name is the textual representation of this plottable as it is displayed in the legend + (\ref QCPLegend). It may contain any UTF-8 characters, including newlines. +*/ +void QCPAbstractPlottable::setName(const QString &name) +{ + mName = name; +} + +/*! + Sets whether fills of this plottable are drawn antialiased or not. + + Note that this setting may be overridden by \ref QCustomPlot::setAntialiasedElements and \ref + QCustomPlot::setNotAntialiasedElements. +*/ +void QCPAbstractPlottable::setAntialiasedFill(bool enabled) +{ + mAntialiasedFill = enabled; +} + +/*! + Sets whether the scatter symbols of this plottable are drawn antialiased or not. + + Note that this setting may be overridden by \ref QCustomPlot::setAntialiasedElements and \ref + QCustomPlot::setNotAntialiasedElements. +*/ +void QCPAbstractPlottable::setAntialiasedScatters(bool enabled) +{ + mAntialiasedScatters = enabled; +} + +/*! + Sets whether the error bars of this plottable are drawn antialiased or not. + + Note that this setting may be overridden by \ref QCustomPlot::setAntialiasedElements and \ref + QCustomPlot::setNotAntialiasedElements. +*/ +void QCPAbstractPlottable::setAntialiasedErrorBars(bool enabled) +{ + mAntialiasedErrorBars = enabled; +} + + +/*! + The pen is used to draw basic lines that make up the plottable representation in the + plot. + + For example, the \ref QCPGraph subclass draws its graph lines with this pen. + + \see setBrush +*/ +void QCPAbstractPlottable::setPen(const QPen &pen) +{ + mPen = pen; +} + +/*! + When the plottable is selected, this pen is used to draw basic lines instead of the normal + pen set via \ref setPen. + + \see setSelected, setSelectable, setSelectedBrush, selectTest +*/ +void QCPAbstractPlottable::setSelectedPen(const QPen &pen) +{ + mSelectedPen = pen; +} + +/*! + The brush is used to draw basic fills of the plottable representation in the + plot. The Fill can be a color, gradient or texture, see the usage of QBrush. + + For example, the \ref QCPGraph subclass draws the fill under the graph with this brush, when + it's not set to Qt::NoBrush. + + \see setPen +*/ +void QCPAbstractPlottable::setBrush(const QBrush &brush) +{ + mBrush = brush; +} + +/*! + When the plottable is selected, this brush is used to draw fills instead of the normal + brush set via \ref setBrush. + + \see setSelected, setSelectable, setSelectedPen, selectTest +*/ +void QCPAbstractPlottable::setSelectedBrush(const QBrush &brush) +{ + mSelectedBrush = brush; +} + +/*! + The key axis of a plottable can be set to any axis of a QCustomPlot, as long as it is orthogonal + to the plottable's value axis. This function performs no checks to make sure this is the case. + The typical mathematical choice is to use the x-axis (QCustomPlot::xAxis) as key axis and the + y-axis (QCustomPlot::yAxis) as value axis. + + Normally, the key and value axes are set in the constructor of the plottable (or \ref + QCustomPlot::addGraph when working with QCPGraphs through the dedicated graph interface). + + \see setValueAxis +*/ +void QCPAbstractPlottable::setKeyAxis(QCPAxis *axis) +{ + mKeyAxis = axis; +} + +/*! + The value axis of a plottable can be set to any axis of a QCustomPlot, as long as it is + orthogonal to the plottable's key axis. This function performs no checks to make sure this is the + case. The typical mathematical choice is to use the x-axis (QCustomPlot::xAxis) as key axis and + the y-axis (QCustomPlot::yAxis) as value axis. + + Normally, the key and value axes are set in the constructor of the plottable (or \ref + QCustomPlot::addGraph when working with QCPGraphs through the dedicated graph interface). + + \see setKeyAxis +*/ +void QCPAbstractPlottable::setValueAxis(QCPAxis *axis) +{ + mValueAxis = axis; +} + +/*! + Sets whether the user can (de-)select this plottable by clicking on the QCustomPlot surface. + (When \ref QCustomPlot::setInteractions contains iSelectPlottables.) + + However, even when \a selectable was set to false, it is possible to set the selection manually, + by calling \ref setSelected directly. + + \see setSelected +*/ +void QCPAbstractPlottable::setSelectable(bool selectable) +{ + if (mSelectable != selectable) + { + mSelectable = selectable; + emit selectableChanged(mSelectable); + } +} + +/*! + Sets whether this plottable is selected or not. When selected, it uses a different pen and brush + to draw its lines and fills, see \ref setSelectedPen and \ref setSelectedBrush. + + The entire selection mechanism for plottables is handled automatically when \ref + QCustomPlot::setInteractions contains iSelectPlottables. You only need to call this function when + you wish to change the selection state manually. + + This function can change the selection state even when \ref setSelectable was set to false. + + emits the \ref selectionChanged signal when \a selected is different from the previous selection state. + + \see setSelectable, selectTest +*/ +void QCPAbstractPlottable::setSelected(bool selected) +{ + if (mSelected != selected) + { + mSelected = selected; + emit selectionChanged(mSelected); + } +} + +/*! + Rescales the key and value axes associated with this plottable to contain all displayed data, so + the whole plottable is visible. If the scaling of an axis is logarithmic, rescaleAxes will make + sure not to rescale to an illegal range i.e. a range containing different signs and/or zero. + Instead it will stay in the current sign domain and ignore all parts of the plottable that lie + outside of that domain. + + \a onlyEnlarge makes sure the ranges are only expanded, never reduced. So it's possible to show + multiple plottables in their entirety by multiple calls to rescaleAxes where the first call has + \a onlyEnlarge set to false (the default), and all subsequent set to true. + + \see rescaleKeyAxis, rescaleValueAxis, QCustomPlot::rescaleAxes, QCPAxis::rescale +*/ +void QCPAbstractPlottable::rescaleAxes(bool onlyEnlarge) const +{ + rescaleKeyAxis(onlyEnlarge); + rescaleValueAxis(onlyEnlarge); +} + +/*! + Rescales the key axis of the plottable so the whole plottable is visible. + + See \ref rescaleAxes for detailed behaviour. +*/ +void QCPAbstractPlottable::rescaleKeyAxis(bool onlyEnlarge) const +{ + QCPAxis *keyAxis = mKeyAxis.data(); + if (!keyAxis) { qDebug() << Q_FUNC_INFO << "invalid key axis"; return; } + + SignDomain signDomain = sdBoth; + if (keyAxis->scaleType() == QCPAxis::stLogarithmic) + signDomain = (keyAxis->range().upper < 0 ? sdNegative : sdPositive); + + bool foundRange; + QCPRange newRange = getKeyRange(foundRange, signDomain); + if (foundRange) + { + if (onlyEnlarge) + newRange.expand(keyAxis->range()); + if (!QCPRange::validRange(newRange)) // likely due to range being zero (plottable has only constant data in this axis dimension), shift current range to at least center the plottable + { + double center = (newRange.lower+newRange.upper)*0.5; // upper and lower should be equal anyway, but just to make sure, incase validRange returned false for other reason + if (keyAxis->scaleType() == QCPAxis::stLinear) + { + newRange.lower = center-keyAxis->range().size()/2.0; + newRange.upper = center+keyAxis->range().size()/2.0; + } else // scaleType() == stLogarithmic + { + newRange.lower = center/qSqrt(keyAxis->range().upper/keyAxis->range().lower); + newRange.upper = center*qSqrt(keyAxis->range().upper/keyAxis->range().lower); + } + } + keyAxis->setRange(newRange); + } +} + +/*! + Rescales the value axis of the plottable so the whole plottable is visible. + + Returns true if the axis was actually scaled. This might not be the case if this plottable has an + invalid range, e.g. because it has no data points. + + See \ref rescaleAxes for detailed behaviour. +*/ +void QCPAbstractPlottable::rescaleValueAxis(bool onlyEnlarge) const +{ + QCPAxis *valueAxis = mValueAxis.data(); + if (!valueAxis) { qDebug() << Q_FUNC_INFO << "invalid value axis"; return; } + + SignDomain signDomain = sdBoth; + if (valueAxis->scaleType() == QCPAxis::stLogarithmic) + signDomain = (valueAxis->range().upper < 0 ? sdNegative : sdPositive); + + bool foundRange; + QCPRange newRange = getValueRange(foundRange, signDomain); + if (foundRange) + { + if (onlyEnlarge) + newRange.expand(valueAxis->range()); + if (!QCPRange::validRange(newRange)) // likely due to range being zero (plottable has only constant data in this axis dimension), shift current range to at least center the plottable + { + double center = (newRange.lower+newRange.upper)*0.5; // upper and lower should be equal anyway, but just to make sure, incase validRange returned false for other reason + if (valueAxis->scaleType() == QCPAxis::stLinear) + { + newRange.lower = center-valueAxis->range().size()/2.0; + newRange.upper = center+valueAxis->range().size()/2.0; + } else // scaleType() == stLogarithmic + { + newRange.lower = center/qSqrt(valueAxis->range().upper/valueAxis->range().lower); + newRange.upper = center*qSqrt(valueAxis->range().upper/valueAxis->range().lower); + } + } + valueAxis->setRange(newRange); + } +} + +/*! + Adds this plottable to the legend of the parent QCustomPlot (QCustomPlot::legend). + + Normally, a QCPPlottableLegendItem is created and inserted into the legend. If the plottable + needs a more specialized representation in the legend, this function will take this into account + and instead create the specialized subclass of QCPAbstractLegendItem. + + Returns true on success, i.e. when the legend exists and a legend item associated with this plottable isn't already in + the legend. + + \see removeFromLegend, QCPLegend::addItem +*/ +bool QCPAbstractPlottable::addToLegend() +{ + if (!mParentPlot || !mParentPlot->legend) + return false; + + if (!mParentPlot->legend->hasItemWithPlottable(this)) + { + mParentPlot->legend->addItem(new QCPPlottableLegendItem(mParentPlot->legend, this)); + return true; + } else + return false; +} + +/*! + Removes the plottable from the legend of the parent QCustomPlot. This means the + QCPAbstractLegendItem (usually a QCPPlottableLegendItem) that is associated with this plottable + is removed. + + Returns true on success, i.e. if the legend exists and a legend item associated with this + plottable was found and removed. + + \see addToLegend, QCPLegend::removeItem +*/ +bool QCPAbstractPlottable::removeFromLegend() const +{ + if (!mParentPlot->legend) + return false; + + if (QCPPlottableLegendItem *lip = mParentPlot->legend->itemWithPlottable(this)) + return mParentPlot->legend->removeItem(lip); + else + return false; +} + +/* inherits documentation from base class */ +QRect QCPAbstractPlottable::clipRect() const +{ + if (mKeyAxis && mValueAxis) + return mKeyAxis.data()->axisRect()->rect() & mValueAxis.data()->axisRect()->rect(); + else + return QRect(); +} + +/* inherits documentation from base class */ +QCP::Interaction QCPAbstractPlottable::selectionCategory() const +{ + return QCP::iSelectPlottables; +} + +/*! \internal + + Convenience function for transforming a key/value pair to pixels on the QCustomPlot surface, + taking the orientations of the axes associated with this plottable into account (e.g. whether key + represents x or y). + + \a key and \a value are transformed to the coodinates in pixels and are written to \a x and \a y. + + \see pixelsToCoords, QCPAxis::coordToPixel +*/ +void QCPAbstractPlottable::coordsToPixels(double key, double value, double &x, double &y) const +{ + QCPAxis *keyAxis = mKeyAxis.data(); + QCPAxis *valueAxis = mValueAxis.data(); + if (!keyAxis || !valueAxis) { qDebug() << Q_FUNC_INFO << "invalid key or value axis"; return; } + + if (keyAxis->orientation() == Qt::Horizontal) + { + x = keyAxis->coordToPixel(key); + y = valueAxis->coordToPixel(value); + } else + { + y = keyAxis->coordToPixel(key); + x = valueAxis->coordToPixel(value); + } +} + +/*! \internal + \overload + + Returns the input as pixel coordinates in a QPointF. +*/ +const QPointF QCPAbstractPlottable::coordsToPixels(double key, double value) const +{ + QCPAxis *keyAxis = mKeyAxis.data(); + QCPAxis *valueAxis = mValueAxis.data(); + if (!keyAxis || !valueAxis) { qDebug() << Q_FUNC_INFO << "invalid key or value axis"; return QPointF(); } + + if (keyAxis->orientation() == Qt::Horizontal) + return QPointF(keyAxis->coordToPixel(key), valueAxis->coordToPixel(value)); + else + return QPointF(valueAxis->coordToPixel(value), keyAxis->coordToPixel(key)); +} + +/*! \internal + + Convenience function for transforming a x/y pixel pair on the QCustomPlot surface to plot coordinates, + taking the orientations of the axes associated with this plottable into account (e.g. whether key + represents x or y). + + \a x and \a y are transformed to the plot coodinates and are written to \a key and \a value. + + \see coordsToPixels, QCPAxis::coordToPixel +*/ +void QCPAbstractPlottable::pixelsToCoords(double x, double y, double &key, double &value) const +{ + QCPAxis *keyAxis = mKeyAxis.data(); + QCPAxis *valueAxis = mValueAxis.data(); + if (!keyAxis || !valueAxis) { qDebug() << Q_FUNC_INFO << "invalid key or value axis"; return; } + + if (keyAxis->orientation() == Qt::Horizontal) + { + key = keyAxis->pixelToCoord(x); + value = valueAxis->pixelToCoord(y); + } else + { + key = keyAxis->pixelToCoord(y); + value = valueAxis->pixelToCoord(x); + } +} + +/*! \internal + \overload + + Returns the pixel input \a pixelPos as plot coordinates \a key and \a value. +*/ +void QCPAbstractPlottable::pixelsToCoords(const QPointF &pixelPos, double &key, double &value) const +{ + pixelsToCoords(pixelPos.x(), pixelPos.y(), key, value); +} + +/*! \internal + + Returns the pen that should be used for drawing lines of the plottable. Returns mPen when the + graph is not selected and mSelectedPen when it is. +*/ +QPen QCPAbstractPlottable::mainPen() const +{ + return mSelected ? mSelectedPen : mPen; +} + +/*! \internal + + Returns the brush that should be used for drawing fills of the plottable. Returns mBrush when the + graph is not selected and mSelectedBrush when it is. +*/ +QBrush QCPAbstractPlottable::mainBrush() const +{ + return mSelected ? mSelectedBrush : mBrush; +} + +/*! \internal + + A convenience function to easily set the QPainter::Antialiased hint on the provided \a painter + before drawing plottable lines. + + This is the antialiasing state the painter passed to the \ref draw method is in by default. + + This function takes into account the local setting of the antialiasing flag as well as the + overrides set with \ref QCustomPlot::setAntialiasedElements and \ref + QCustomPlot::setNotAntialiasedElements. + + \see setAntialiased, applyFillAntialiasingHint, applyScattersAntialiasingHint, applyErrorBarsAntialiasingHint +*/ +void QCPAbstractPlottable::applyDefaultAntialiasingHint(QCPPainter *painter) const +{ + applyAntialiasingHint(painter, mAntialiased, QCP::aePlottables); +} + +/*! \internal + + A convenience function to easily set the QPainter::Antialiased hint on the provided \a painter + before drawing plottable fills. + + This function takes into account the local setting of the antialiasing flag as well as the + overrides set with \ref QCustomPlot::setAntialiasedElements and \ref + QCustomPlot::setNotAntialiasedElements. + + \see setAntialiased, applyDefaultAntialiasingHint, applyScattersAntialiasingHint, applyErrorBarsAntialiasingHint +*/ +void QCPAbstractPlottable::applyFillAntialiasingHint(QCPPainter *painter) const +{ + applyAntialiasingHint(painter, mAntialiasedFill, QCP::aeFills); +} + +/*! \internal + + A convenience function to easily set the QPainter::Antialiased hint on the provided \a painter + before drawing plottable scatter points. + + This function takes into account the local setting of the antialiasing flag as well as the + overrides set with \ref QCustomPlot::setAntialiasedElements and \ref + QCustomPlot::setNotAntialiasedElements. + + \see setAntialiased, applyFillAntialiasingHint, applyDefaultAntialiasingHint, applyErrorBarsAntialiasingHint +*/ +void QCPAbstractPlottable::applyScattersAntialiasingHint(QCPPainter *painter) const +{ + applyAntialiasingHint(painter, mAntialiasedScatters, QCP::aeScatters); +} + +/*! \internal + + A convenience function to easily set the QPainter::Antialiased hint on the provided \a painter + before drawing plottable error bars. + + This function takes into account the local setting of the antialiasing flag as well as the + overrides set with \ref QCustomPlot::setAntialiasedElements and \ref + QCustomPlot::setNotAntialiasedElements. + + \see setAntialiased, applyFillAntialiasingHint, applyScattersAntialiasingHint, applyDefaultAntialiasingHint +*/ +void QCPAbstractPlottable::applyErrorBarsAntialiasingHint(QCPPainter *painter) const +{ + applyAntialiasingHint(painter, mAntialiasedErrorBars, QCP::aeErrorBars); +} + +/*! \internal + + Finds the shortest squared distance of \a point to the line segment defined by \a start and \a + end. + + This function may be used to help with the implementation of the \ref selectTest function for + specific plottables. + + \note This function is identical to QCPAbstractItem::distSqrToLine +*/ +double QCPAbstractPlottable::distSqrToLine(const QPointF &start, const QPointF &end, const QPointF &point) const +{ + QVector2D a(start); + QVector2D b(end); + QVector2D p(point); + QVector2D v(b-a); + + double vLengthSqr = v.lengthSquared(); + if (!qFuzzyIsNull(vLengthSqr)) + { + double mu = QVector2D::dotProduct(p-a, v)/vLengthSqr; + if (mu < 0) + return (a-p).lengthSquared(); + else if (mu > 1) + return (b-p).lengthSquared(); + else + return ((a + mu*v)-p).lengthSquared(); + } else + return (a-p).lengthSquared(); +} + +/* inherits documentation from base class */ +void QCPAbstractPlottable::selectEvent(QMouseEvent *event, bool additive, const QVariant &details, bool *selectionStateChanged) +{ + Q_UNUSED(event) + Q_UNUSED(details) + if (mSelectable) + { + bool selBefore = mSelected; + setSelected(additive ? !mSelected : true); + if (selectionStateChanged) + *selectionStateChanged = mSelected != selBefore; + } +} + +/* inherits documentation from base class */ +void QCPAbstractPlottable::deselectEvent(bool *selectionStateChanged) +{ + if (mSelectable) + { + bool selBefore = mSelected; + setSelected(false); + if (selectionStateChanged) + *selectionStateChanged = mSelected != selBefore; + } +} + + +//////////////////////////////////////////////////////////////////////////////////////////////////// +//////////////////// QCPItemAnchor +//////////////////////////////////////////////////////////////////////////////////////////////////// + +/*! \class QCPItemAnchor + \brief An anchor of an item to which positions can be attached to. + + An item (QCPAbstractItem) may have one or more anchors. Unlike QCPItemPosition, an anchor doesn't + control anything on its item, but provides a way to tie other items via their positions to the + anchor. + + For example, a QCPItemRect is defined by its positions \a topLeft and \a bottomRight. + Additionally it has various anchors like \a top, \a topRight or \a bottomLeft etc. So you can + attach the \a start (which is a QCPItemPosition) of a QCPItemLine to one of the anchors by + calling QCPItemPosition::setParentAnchor on \a start, passing the wanted anchor of the + QCPItemRect. This way the start of the line will now always follow the respective anchor location + on the rect item. + + Note that QCPItemPosition derives from QCPItemAnchor, so every position can also serve as an + anchor to other positions. + + To learn how to provide anchors in your own item subclasses, see the subclassing section of the + QCPAbstractItem documentation. +*/ + +/* start documentation of inline functions */ + +/*! \fn virtual QCPItemPosition *QCPItemAnchor::toQCPItemPosition() + + Returns 0 if this instance is merely a QCPItemAnchor, and a valid pointer of type QCPItemPosition* if + it actually is a QCPItemPosition (which is a subclass of QCPItemAnchor). + + This safe downcast functionality could also be achieved with a dynamic_cast. However, QCustomPlot avoids + dynamic_cast to work with projects that don't have RTTI support enabled (e.g. -fno-rtti flag with + gcc compiler). +*/ + +/* end documentation of inline functions */ + +/*! + Creates a new QCPItemAnchor. You shouldn't create QCPItemAnchor instances directly, even if + you want to make a new item subclass. Use \ref QCPAbstractItem::createAnchor instead, as + explained in the subclassing section of the QCPAbstractItem documentation. +*/ +QCPItemAnchor::QCPItemAnchor(QCustomPlot *parentPlot, QCPAbstractItem *parentItem, const QString name, int anchorId) : + mName(name), + mParentPlot(parentPlot), + mParentItem(parentItem), + mAnchorId(anchorId) +{ +} + +QCPItemAnchor::~QCPItemAnchor() +{ + // unregister as parent at children: + foreach (QCPItemPosition *child, mChildrenX.toList()) + { + if (child->parentAnchorX() == this) + child->setParentAnchorX(0); // this acts back on this anchor and child removes itself from mChildrenX + } + foreach (QCPItemPosition *child, mChildrenY.toList()) + { + if (child->parentAnchorY() == this) + child->setParentAnchorY(0); // this acts back on this anchor and child removes itself from mChildrenY + } +} + +/*! + Returns the final absolute pixel position of the QCPItemAnchor on the QCustomPlot surface. + + The pixel information is internally retrieved via QCPAbstractItem::anchorPixelPosition of the + parent item, QCPItemAnchor is just an intermediary. +*/ +QPointF QCPItemAnchor::pixelPoint() const +{ + if (mParentItem) + { + if (mAnchorId > -1) + { + return mParentItem->anchorPixelPoint(mAnchorId); + } else + { + qDebug() << Q_FUNC_INFO << "no valid anchor id set:" << mAnchorId; + return QPointF(); + } + } else + { + qDebug() << Q_FUNC_INFO << "no parent item set"; + return QPointF(); + } +} + +/*! \internal + + Adds \a pos to the childX list of this anchor, which keeps track of which children use this + anchor as parent anchor for the respective coordinate. This is necessary to notify the children + prior to destruction of the anchor. + + Note that this function does not change the parent setting in \a pos. +*/ +void QCPItemAnchor::addChildX(QCPItemPosition *pos) +{ + if (!mChildrenX.contains(pos)) + mChildrenX.insert(pos); + else + qDebug() << Q_FUNC_INFO << "provided pos is child already" << reinterpret_cast(pos); +} + +/*! \internal + + Removes \a pos from the childX list of this anchor. + + Note that this function does not change the parent setting in \a pos. +*/ +void QCPItemAnchor::removeChildX(QCPItemPosition *pos) +{ + if (!mChildrenX.remove(pos)) + qDebug() << Q_FUNC_INFO << "provided pos isn't child" << reinterpret_cast(pos); +} + +/*! \internal + + Adds \a pos to the childY list of this anchor, which keeps track of which children use this + anchor as parent anchor for the respective coordinate. This is necessary to notify the children + prior to destruction of the anchor. + + Note that this function does not change the parent setting in \a pos. +*/ +void QCPItemAnchor::addChildY(QCPItemPosition *pos) +{ + if (!mChildrenY.contains(pos)) + mChildrenY.insert(pos); + else + qDebug() << Q_FUNC_INFO << "provided pos is child already" << reinterpret_cast(pos); +} + +/*! \internal + + Removes \a pos from the childY list of this anchor. + + Note that this function does not change the parent setting in \a pos. +*/ +void QCPItemAnchor::removeChildY(QCPItemPosition *pos) +{ + if (!mChildrenY.remove(pos)) + qDebug() << Q_FUNC_INFO << "provided pos isn't child" << reinterpret_cast(pos); +} + + +//////////////////////////////////////////////////////////////////////////////////////////////////// +//////////////////// QCPItemPosition +//////////////////////////////////////////////////////////////////////////////////////////////////// + +/*! \class QCPItemPosition + \brief Manages the position of an item. + + Every item has at least one public QCPItemPosition member pointer which provides ways to position the + item on the QCustomPlot surface. Some items have multiple positions, for example QCPItemRect has two: + \a topLeft and \a bottomRight. + + QCPItemPosition has a type (\ref PositionType) that can be set with \ref setType. This type + defines how coordinates passed to \ref setCoords are to be interpreted, e.g. as absolute pixel + coordinates, as plot coordinates of certain axes, etc. For more advanced plots it is also + possible to assign different types per X/Y coordinate of the position (see \ref setTypeX, \ref + setTypeY). This way an item could be positioned at a fixed pixel distance from the top in the Y + direction, while following a plot coordinate in the X direction. + + A QCPItemPosition may have a parent QCPItemAnchor, see \ref setParentAnchor. This way you can tie + multiple items together. If the QCPItemPosition has a parent, its coordinates (\ref setCoords) + are considered to be absolute pixels in the reference frame of the parent anchor, where (0, 0) + means directly ontop of the parent anchor. For example, You could attach the \a start position of + a QCPItemLine to the \a bottom anchor of a QCPItemText to make the starting point of the line + always be centered under the text label, no matter where the text is moved to. For more advanced + plots, it is possible to assign different parent anchors per X/Y coordinate of the position, see + \ref setParentAnchorX, \ref setParentAnchorY. This way an item could follow another item in the X + direction but stay at a fixed position in the Y direction. Or even follow item A in X, and item B + in Y. + + Note that every QCPItemPosition inherits from QCPItemAnchor and thus can itself be used as parent + anchor for other positions. + + To set the apparent pixel position on the QCustomPlot surface directly, use \ref setPixelPoint. This + works no matter what type this QCPItemPosition is or what parent-child situation it is in, as \ref + setPixelPoint transforms the coordinates appropriately, to make the position appear at the specified + pixel values. +*/ + +/* start documentation of inline functions */ + +/*! \fn QCPItemPosition::PositionType *QCPItemPosition::type() const + + Returns the current position type. + + If different types were set for X and Y (\ref setTypeX, \ref setTypeY), this method returns the + type of the X coordinate. In that case rather use \a typeX() and \a typeY(). + + \see setType +*/ + +/*! \fn QCPItemAnchor *QCPItemPosition::parentAnchor() const + + Returns the current parent anchor. + + If different parent anchors were set for X and Y (\ref setParentAnchorX, \ref setParentAnchorY), + this method returns the parent anchor of the Y coordinate. In that case rather use \a + parentAnchorX() and \a parentAnchorY(). + + \see setParentAnchor +*/ + +/* end documentation of inline functions */ + +/*! + Creates a new QCPItemPosition. You shouldn't create QCPItemPosition instances directly, even if + you want to make a new item subclass. Use \ref QCPAbstractItem::createPosition instead, as + explained in the subclassing section of the QCPAbstractItem documentation. +*/ +QCPItemPosition::QCPItemPosition(QCustomPlot *parentPlot, QCPAbstractItem *parentItem, const QString name) : + QCPItemAnchor(parentPlot, parentItem, name), + mPositionTypeX(ptAbsolute), + mPositionTypeY(ptAbsolute), + mKey(0), + mValue(0), + mParentAnchorX(0), + mParentAnchorY(0) +{ +} + +QCPItemPosition::~QCPItemPosition() +{ + // unregister as parent at children: + // Note: this is done in ~QCPItemAnchor again, but it's important QCPItemPosition does it itself, because only then + // the setParentAnchor(0) call the correct QCPItemPosition::pixelPoint function instead of QCPItemAnchor::pixelPoint + foreach (QCPItemPosition *child, mChildrenX.toList()) + { + if (child->parentAnchorX() == this) + child->setParentAnchorX(0); // this acts back on this anchor and child removes itself from mChildrenX + } + foreach (QCPItemPosition *child, mChildrenY.toList()) + { + if (child->parentAnchorY() == this) + child->setParentAnchorY(0); // this acts back on this anchor and child removes itself from mChildrenY + } + // unregister as child in parent: + if (mParentAnchorX) + mParentAnchorX->removeChildX(this); + if (mParentAnchorY) + mParentAnchorY->removeChildY(this); +} + +/* can't make this a header inline function, because QPointer breaks with forward declared types, see QTBUG-29588 */ +QCPAxisRect *QCPItemPosition::axisRect() const +{ + return mAxisRect.data(); +} + +/*! + Sets the type of the position. The type defines how the coordinates passed to \ref setCoords + should be handled and how the QCPItemPosition should behave in the plot. + + The possible values for \a type can be separated in two main categories: + + \li The position is regarded as a point in plot coordinates. This corresponds to \ref ptPlotCoords + and requires two axes that define the plot coordinate system. They can be specified with \ref setAxes. + By default, the QCustomPlot's x- and yAxis are used. + + \li The position is fixed on the QCustomPlot surface, i.e. independent of axis ranges. This + corresponds to all other types, i.e. \ref ptAbsolute, \ref ptViewportRatio and \ref + ptAxisRectRatio. They differ only in the way the absolute position is described, see the + documentation of \ref PositionType for details. For \ref ptAxisRectRatio, note that you can specify + the axis rect with \ref setAxisRect. By default this is set to the main axis rect. + + Note that the position type \ref ptPlotCoords is only available (and sensible) when the position + has no parent anchor (\ref setParentAnchor). + + If the type is changed, the apparent pixel position on the plot is preserved. This means + the coordinates as retrieved with coords() and set with \ref setCoords may change in the process. + + This method sets the type for both X and Y directions. It is also possible to set different types + for X and Y, see \ref setTypeX, \ref setTypeY. +*/ +void QCPItemPosition::setType(QCPItemPosition::PositionType type) +{ + setTypeX(type); + setTypeY(type); +} + +/*! + This method sets the position type of the X coordinate to \a type. + + For a detailed description of what a position type is, see the documentation of \ref setType. + + \see setType, setTypeY +*/ +void QCPItemPosition::setTypeX(QCPItemPosition::PositionType type) +{ + if (mPositionTypeX != type) + { + // if switching from or to coordinate type that isn't valid (e.g. because axes or axis rect + // were deleted), don't try to recover the pixelPoint() because it would output a qDebug warning. + bool retainPixelPosition = true; + if ((mPositionTypeX == ptPlotCoords || type == ptPlotCoords) && (!mKeyAxis || !mValueAxis)) + retainPixelPosition = false; + if ((mPositionTypeX == ptAxisRectRatio || type == ptAxisRectRatio) && (!mAxisRect)) + retainPixelPosition = false; + + QPointF pixel; + if (retainPixelPosition) + pixel = pixelPoint(); + + mPositionTypeX = type; + + if (retainPixelPosition) + setPixelPoint(pixel); + } +} + +/*! + This method sets the position type of the Y coordinate to \a type. + + For a detailed description of what a position type is, see the documentation of \ref setType. + + \see setType, setTypeX +*/ +void QCPItemPosition::setTypeY(QCPItemPosition::PositionType type) +{ + if (mPositionTypeY != type) + { + // if switching from or to coordinate type that isn't valid (e.g. because axes or axis rect + // were deleted), don't try to recover the pixelPoint() because it would output a qDebug warning. + bool retainPixelPosition = true; + if ((mPositionTypeY == ptPlotCoords || type == ptPlotCoords) && (!mKeyAxis || !mValueAxis)) + retainPixelPosition = false; + if ((mPositionTypeY == ptAxisRectRatio || type == ptAxisRectRatio) && (!mAxisRect)) + retainPixelPosition = false; + + QPointF pixel; + if (retainPixelPosition) + pixel = pixelPoint(); + + mPositionTypeY = type; + + if (retainPixelPosition) + setPixelPoint(pixel); + } +} + +/*! + Sets the parent of this QCPItemPosition to \a parentAnchor. This means the position will now + follow any position changes of the anchor. The local coordinate system of positions with a parent + anchor always is absolute pixels, with (0, 0) being exactly on top of the parent anchor. (Hence + the type shouldn't be set to \ref ptPlotCoords for positions with parent anchors.) + + if \a keepPixelPosition is true, the current pixel position of the QCPItemPosition is preserved + during reparenting. If it's set to false, the coordinates are set to (0, 0), i.e. the position + will be exactly on top of the parent anchor. + + To remove this QCPItemPosition from any parent anchor, set \a parentAnchor to 0. + + If the QCPItemPosition previously had no parent and the type is \ref ptPlotCoords, the type is + set to \ref ptAbsolute, to keep the position in a valid state. + + This method sets the parent anchor for both X and Y directions. It is also possible to set + different parents for X and Y, see \ref setParentAnchorX, \ref setParentAnchorY. +*/ +bool QCPItemPosition::setParentAnchor(QCPItemAnchor *parentAnchor, bool keepPixelPosition) +{ + bool successX = setParentAnchorX(parentAnchor, keepPixelPosition); + bool successY = setParentAnchorY(parentAnchor, keepPixelPosition); + return successX && successY; +} + +/*! + This method sets the parent anchor of the X coordinate to \a parentAnchor. + + For a detailed description of what a parent anchor is, see the documentation of \ref setParentAnchor. + + \see setParentAnchor, setParentAnchorY +*/ +bool QCPItemPosition::setParentAnchorX(QCPItemAnchor *parentAnchor, bool keepPixelPosition) +{ + // make sure self is not assigned as parent: + if (parentAnchor == this) + { + qDebug() << Q_FUNC_INFO << "can't set self as parent anchor" << reinterpret_cast(parentAnchor); + return false; + } + // make sure no recursive parent-child-relationships are created: + QCPItemAnchor *currentParent = parentAnchor; + while (currentParent) + { + if (QCPItemPosition *currentParentPos = currentParent->toQCPItemPosition()) + { + // is a QCPItemPosition, might have further parent, so keep iterating + if (currentParentPos == this) + { + qDebug() << Q_FUNC_INFO << "can't create recursive parent-child-relationship" << reinterpret_cast(parentAnchor); + return false; + } + currentParent = currentParentPos->parentAnchorX(); + } else + { + // is a QCPItemAnchor, can't have further parent. Now make sure the parent items aren't the + // same, to prevent a position being child of an anchor which itself depends on the position, + // because they're both on the same item: + if (currentParent->mParentItem == mParentItem) + { + qDebug() << Q_FUNC_INFO << "can't set parent to be an anchor which itself depends on this position" << reinterpret_cast(parentAnchor); + return false; + } + break; + } + } + + // if previously no parent set and PosType is still ptPlotCoords, set to ptAbsolute: + if (!mParentAnchorX && mPositionTypeX == ptPlotCoords) + setTypeX(ptAbsolute); + + // save pixel position: + QPointF pixelP; + if (keepPixelPosition) + pixelP = pixelPoint(); + // unregister at current parent anchor: + if (mParentAnchorX) + mParentAnchorX->removeChildX(this); + // register at new parent anchor: + if (parentAnchor) + parentAnchor->addChildX(this); + mParentAnchorX = parentAnchor; + // restore pixel position under new parent: + if (keepPixelPosition) + setPixelPoint(pixelP); + else + setCoords(0, coords().y()); + return true; +} + +/*! + This method sets the parent anchor of the Y coordinate to \a parentAnchor. + + For a detailed description of what a parent anchor is, see the documentation of \ref setParentAnchor. + + \see setParentAnchor, setParentAnchorX +*/ +bool QCPItemPosition::setParentAnchorY(QCPItemAnchor *parentAnchor, bool keepPixelPosition) +{ + // make sure self is not assigned as parent: + if (parentAnchor == this) + { + qDebug() << Q_FUNC_INFO << "can't set self as parent anchor" << reinterpret_cast(parentAnchor); + return false; + } + // make sure no recursive parent-child-relationships are created: + QCPItemAnchor *currentParent = parentAnchor; + while (currentParent) + { + if (QCPItemPosition *currentParentPos = currentParent->toQCPItemPosition()) + { + // is a QCPItemPosition, might have further parent, so keep iterating + if (currentParentPos == this) + { + qDebug() << Q_FUNC_INFO << "can't create recursive parent-child-relationship" << reinterpret_cast(parentAnchor); + return false; + } + currentParent = currentParentPos->parentAnchorY(); + } else + { + // is a QCPItemAnchor, can't have further parent. Now make sure the parent items aren't the + // same, to prevent a position being child of an anchor which itself depends on the position, + // because they're both on the same item: + if (currentParent->mParentItem == mParentItem) + { + qDebug() << Q_FUNC_INFO << "can't set parent to be an anchor which itself depends on this position" << reinterpret_cast(parentAnchor); + return false; + } + break; + } + } + + // if previously no parent set and PosType is still ptPlotCoords, set to ptAbsolute: + if (!mParentAnchorY && mPositionTypeY == ptPlotCoords) + setTypeY(ptAbsolute); + + // save pixel position: + QPointF pixelP; + if (keepPixelPosition) + pixelP = pixelPoint(); + // unregister at current parent anchor: + if (mParentAnchorY) + mParentAnchorY->removeChildY(this); + // register at new parent anchor: + if (parentAnchor) + parentAnchor->addChildY(this); + mParentAnchorY = parentAnchor; + // restore pixel position under new parent: + if (keepPixelPosition) + setPixelPoint(pixelP); + else + setCoords(coords().x(), 0); + return true; +} + +/*! + Sets the coordinates of this QCPItemPosition. What the coordinates mean, is defined by the type + (\ref setType, \ref setTypeX, \ref setTypeY). + + For example, if the type is \ref ptAbsolute, \a key and \a value mean the x and y pixel position + on the QCustomPlot surface. In that case the origin (0, 0) is in the top left corner of the + QCustomPlot viewport. If the type is \ref ptPlotCoords, \a key and \a value mean a point in the + plot coordinate system defined by the axes set by \ref setAxes. By default those are the + QCustomPlot's xAxis and yAxis. See the documentation of \ref setType for other available + coordinate types and their meaning. + + If different types were configured for X and Y (\ref setTypeX, \ref setTypeY), \a key and \a + value must also be provided in the different coordinate systems. Here, the X type refers to \a + key, and the Y type refers to \a value. + + \see setPixelPoint +*/ +void QCPItemPosition::setCoords(double key, double value) +{ + mKey = key; + mValue = value; +} + +/*! \overload + + Sets the coordinates as a QPointF \a pos where pos.x has the meaning of \a key and pos.y the + meaning of \a value of the \ref setCoords(double key, double value) method. +*/ +void QCPItemPosition::setCoords(const QPointF &pos) +{ + setCoords(pos.x(), pos.y()); +} + +/*! + Returns the final absolute pixel position of the QCPItemPosition on the QCustomPlot surface. It + includes all effects of type (\ref setType) and possible parent anchors (\ref setParentAnchor). + + \see setPixelPoint +*/ +QPointF QCPItemPosition::pixelPoint() const +{ + QPointF result; + + // determine X: + switch (mPositionTypeX) + { + case ptAbsolute: + { + result.rx() = mKey; + if (mParentAnchorX) + result.rx() += mParentAnchorX->pixelPoint().x(); + break; + } + case ptViewportRatio: + { + result.rx() = mKey*mParentPlot->viewport().width(); + if (mParentAnchorX) + result.rx() += mParentAnchorX->pixelPoint().x(); + else + result.rx() += mParentPlot->viewport().left(); + break; + } + case ptAxisRectRatio: + { + if (mAxisRect) + { + result.rx() = mKey*mAxisRect.data()->width(); + if (mParentAnchorX) + result.rx() += mParentAnchorX->pixelPoint().x(); + else + result.rx() += mAxisRect.data()->left(); + } else + qDebug() << Q_FUNC_INFO << "Item position type x is ptAxisRectRatio, but no axis rect was defined"; + break; + } + case ptPlotCoords: + { + if (mKeyAxis && mKeyAxis.data()->orientation() == Qt::Horizontal) + result.rx() = mKeyAxis.data()->coordToPixel(mKey); + else if (mValueAxis && mValueAxis.data()->orientation() == Qt::Horizontal) + result.rx() = mValueAxis.data()->coordToPixel(mValue); + else + qDebug() << Q_FUNC_INFO << "Item position type x is ptPlotCoords, but no axes were defined"; + break; + } + } + + // determine Y: + switch (mPositionTypeY) + { + case ptAbsolute: + { + result.ry() = mValue; + if (mParentAnchorY) + result.ry() += mParentAnchorY->pixelPoint().y(); + break; + } + case ptViewportRatio: + { + result.ry() = mValue*mParentPlot->viewport().height(); + if (mParentAnchorY) + result.ry() += mParentAnchorY->pixelPoint().y(); + else + result.ry() += mParentPlot->viewport().top(); + break; + } + case ptAxisRectRatio: + { + if (mAxisRect) + { + result.ry() = mValue*mAxisRect.data()->height(); + if (mParentAnchorY) + result.ry() += mParentAnchorY->pixelPoint().y(); + else + result.ry() += mAxisRect.data()->top(); + } else + qDebug() << Q_FUNC_INFO << "Item position type y is ptAxisRectRatio, but no axis rect was defined"; + break; + } + case ptPlotCoords: + { + if (mKeyAxis && mKeyAxis.data()->orientation() == Qt::Vertical) + result.ry() = mKeyAxis.data()->coordToPixel(mKey); + else if (mValueAxis && mValueAxis.data()->orientation() == Qt::Vertical) + result.ry() = mValueAxis.data()->coordToPixel(mValue); + else + qDebug() << Q_FUNC_INFO << "Item position type y is ptPlotCoords, but no axes were defined"; + break; + } + } + + return result; +} + +/*! + When \ref setType is \ref ptPlotCoords, this function may be used to specify the axes the + coordinates set with \ref setCoords relate to. By default they are set to the initial xAxis and + yAxis of the QCustomPlot. +*/ +void QCPItemPosition::setAxes(QCPAxis *keyAxis, QCPAxis *valueAxis) +{ + mKeyAxis = keyAxis; + mValueAxis = valueAxis; +} + +/*! + When \ref setType is \ref ptAxisRectRatio, this function may be used to specify the axis rect the + coordinates set with \ref setCoords relate to. By default this is set to the main axis rect of + the QCustomPlot. +*/ +void QCPItemPosition::setAxisRect(QCPAxisRect *axisRect) +{ + mAxisRect = axisRect; +} + +/*! + Sets the apparent pixel position. This works no matter what type (\ref setType) this + QCPItemPosition is or what parent-child situation it is in, as coordinates are transformed + appropriately, to make the position finally appear at the specified pixel values. + + Only if the type is \ref ptAbsolute and no parent anchor is set, this function's effect is + identical to that of \ref setCoords. + + \see pixelPoint, setCoords +*/ +void QCPItemPosition::setPixelPoint(const QPointF &pixelPoint) +{ + double x = pixelPoint.x(); + double y = pixelPoint.y(); + + switch (mPositionTypeX) + { + case ptAbsolute: + { + if (mParentAnchorX) + x -= mParentAnchorX->pixelPoint().x(); + break; + } + case ptViewportRatio: + { + if (mParentAnchorX) + x -= mParentAnchorX->pixelPoint().x(); + else + x -= mParentPlot->viewport().left(); + x /= (double)mParentPlot->viewport().width(); + break; + } + case ptAxisRectRatio: + { + if (mAxisRect) + { + if (mParentAnchorX) + x -= mParentAnchorX->pixelPoint().x(); + else + x -= mAxisRect.data()->left(); + x /= (double)mAxisRect.data()->width(); + } else + qDebug() << Q_FUNC_INFO << "Item position type x is ptAxisRectRatio, but no axis rect was defined"; + break; + } + case ptPlotCoords: + { + if (mKeyAxis && mKeyAxis.data()->orientation() == Qt::Horizontal) + x = mKeyAxis.data()->pixelToCoord(x); + else if (mValueAxis && mValueAxis.data()->orientation() == Qt::Horizontal) + y = mValueAxis.data()->pixelToCoord(x); + else + qDebug() << Q_FUNC_INFO << "Item position type x is ptPlotCoords, but no axes were defined"; + break; + } + } + + switch (mPositionTypeY) + { + case ptAbsolute: + { + if (mParentAnchorY) + y -= mParentAnchorY->pixelPoint().y(); + break; + } + case ptViewportRatio: + { + if (mParentAnchorY) + y -= mParentAnchorY->pixelPoint().y(); + else + y -= mParentPlot->viewport().top(); + y /= (double)mParentPlot->viewport().height(); + break; + } + case ptAxisRectRatio: + { + if (mAxisRect) + { + if (mParentAnchorY) + y -= mParentAnchorY->pixelPoint().y(); + else + y -= mAxisRect.data()->top(); + y /= (double)mAxisRect.data()->height(); + } else + qDebug() << Q_FUNC_INFO << "Item position type y is ptAxisRectRatio, but no axis rect was defined"; + break; + } + case ptPlotCoords: + { + if (mKeyAxis && mKeyAxis.data()->orientation() == Qt::Vertical) + x = mKeyAxis.data()->pixelToCoord(y); + else if (mValueAxis && mValueAxis.data()->orientation() == Qt::Vertical) + y = mValueAxis.data()->pixelToCoord(y); + else + qDebug() << Q_FUNC_INFO << "Item position type y is ptPlotCoords, but no axes were defined"; + break; + } + } + + setCoords(x, y); +} + + +//////////////////////////////////////////////////////////////////////////////////////////////////// +//////////////////// QCPAbstractItem +//////////////////////////////////////////////////////////////////////////////////////////////////// + +/*! \class QCPAbstractItem + \brief The abstract base class for all items in a plot. + + In QCustomPlot, items are supplemental graphical elements that are neither plottables + (QCPAbstractPlottable) nor axes (QCPAxis). While plottables are always tied to two axes and thus + plot coordinates, items can also be placed in absolute coordinates independent of any axes. Each + specific item has at least one QCPItemPosition member which controls the positioning. Some items + are defined by more than one coordinate and thus have two or more QCPItemPosition members (For + example, QCPItemRect has \a topLeft and \a bottomRight). + + This abstract base class defines a very basic interface like visibility and clipping. Since this + class is abstract, it can't be instantiated. Use one of the subclasses or create a subclass + yourself to create new items. + + The built-in items are: + + + + + + + + + + +
QCPItemLineA line defined by a start and an end point. May have different ending styles on each side (e.g. arrows).
QCPItemStraightLineA straight line defined by a start and a direction point. Unlike QCPItemLine, the straight line is infinitely long and has no endings.
QCPItemCurveA curve defined by start, end and two intermediate control points. May have different ending styles on each side (e.g. arrows).
QCPItemRectA rectangle
QCPItemEllipseAn ellipse
QCPItemPixmapAn arbitrary pixmap
QCPItemTextA text label
QCPItemBracketA bracket which may be used to reference/highlight certain parts in the plot.
QCPItemTracerAn item that can be attached to a QCPGraph and sticks to its data points, given a key coordinate.
+ + \section items-clipping Clipping + + Items are by default clipped to the main axis rect (they are only visible inside the axis rect). + To make an item visible outside that axis rect, disable clipping via \ref setClipToAxisRect + "setClipToAxisRect(false)". + + On the other hand if you want the item to be clipped to a different axis rect, specify it via + \ref setClipAxisRect. This clipAxisRect property of an item is only used for clipping behaviour, and + in principle is independent of the coordinate axes the item might be tied to via its position + members (\ref QCPItemPosition::setAxes). However, it is common that the axis rect for clipping + also contains the axes used for the item positions. + + \section items-using Using items + + First you instantiate the item you want to use and add it to the plot: + \snippet documentation/doc-code-snippets/mainwindow.cpp qcpitemline-creation-1 + by default, the positions of the item are bound to the x- and y-Axis of the plot. So we can just + set the plot coordinates where the line should start/end: + \snippet documentation/doc-code-snippets/mainwindow.cpp qcpitemline-creation-2 + If we don't want the line to be positioned in plot coordinates but a different coordinate system, + e.g. absolute pixel positions on the QCustomPlot surface, we need to change the position type like this: + \snippet documentation/doc-code-snippets/mainwindow.cpp qcpitemline-creation-3 + Then we can set the coordinates, this time in pixels: + \snippet documentation/doc-code-snippets/mainwindow.cpp qcpitemline-creation-4 + and make the line visible on the entire QCustomPlot, by disabling clipping to the axis rect: + \snippet documentation/doc-code-snippets/mainwindow.cpp qcpitemline-creation-5 + + For more advanced plots, it is even possible to set different types and parent anchors per X/Y + coordinate of an item position, using for example \ref QCPItemPosition::setTypeX or \ref + QCPItemPosition::setParentAnchorX. For details, see the documentation of \ref QCPItemPosition. + + \section items-subclassing Creating own items + + To create an own item, you implement a subclass of QCPAbstractItem. These are the pure + virtual functions, you must implement: + \li \ref selectTest + \li \ref draw + + See the documentation of those functions for what they need to do. + + \subsection items-positioning Allowing the item to be positioned + + As mentioned, item positions are represented by QCPItemPosition members. Let's assume the new item shall + have only one point as its position (as opposed to two like a rect or multiple like a polygon). You then add + a public member of type QCPItemPosition like so: + + \code QCPItemPosition * const myPosition;\endcode + + the const makes sure the pointer itself can't be modified from the user of your new item (the QCPItemPosition + instance it points to, can be modified, of course). + The initialization of this pointer is made easy with the \ref createPosition function. Just assign + the return value of this function to each QCPItemPosition in the constructor of your item. \ref createPosition + takes a string which is the name of the position, typically this is identical to the variable name. + For example, the constructor of QCPItemExample could look like this: + + \code + QCPItemExample::QCPItemExample(QCustomPlot *parentPlot) : + QCPAbstractItem(parentPlot), + myPosition(createPosition("myPosition")) + { + // other constructor code + } + \endcode + + \subsection items-drawing The draw function + + To give your item a visual representation, reimplement the \ref draw function and use the passed + QCPPainter to draw the item. You can retrieve the item position in pixel coordinates from the + position member(s) via \ref QCPItemPosition::pixelPoint. + + To optimize performance you should calculate a bounding rect first (don't forget to take the pen + width into account), check whether it intersects the \ref clipRect, and only draw the item at all + if this is the case. + + \subsection items-selection The selectTest function + + Your implementation of the \ref selectTest function may use the helpers \ref distSqrToLine and + \ref rectSelectTest. With these, the implementation of the selection test becomes significantly + simpler for most items. See the documentation of \ref selectTest for what the function parameters + mean and what the function should return. + + \subsection anchors Providing anchors + + Providing anchors (QCPItemAnchor) starts off like adding a position. First you create a public + member, e.g. + + \code QCPItemAnchor * const bottom;\endcode + + and create it in the constructor with the \ref createAnchor function, assigning it a name and an + anchor id (an integer enumerating all anchors on the item, you may create an own enum for this). + Since anchors can be placed anywhere, relative to the item's position(s), your item needs to + provide the position of every anchor with the reimplementation of the \ref anchorPixelPoint(int + anchorId) function. + + In essence the QCPItemAnchor is merely an intermediary that itself asks your item for the pixel + position when anything attached to the anchor needs to know the coordinates. +*/ + +/* start of documentation of inline functions */ + +/*! \fn QList QCPAbstractItem::positions() const + + Returns all positions of the item in a list. + + \see anchors, position +*/ + +/*! \fn QList QCPAbstractItem::anchors() const + + Returns all anchors of the item in a list. Note that since a position (QCPItemPosition) is always + also an anchor, the list will also contain the positions of this item. + + \see positions, anchor +*/ + +/* end of documentation of inline functions */ +/* start documentation of pure virtual functions */ + +/*! \fn void QCPAbstractItem::draw(QCPPainter *painter) = 0 + \internal + + Draws this item with the provided \a painter. + + The cliprect of the provided painter is set to the rect returned by \ref clipRect before this + function is called. The clipRect depends on the clipping settings defined by \ref + setClipToAxisRect and \ref setClipAxisRect. +*/ + +/* end documentation of pure virtual functions */ +/* start documentation of signals */ + +/*! \fn void QCPAbstractItem::selectionChanged(bool selected) + This signal is emitted when the selection state of this item has changed, either by user interaction + or by a direct call to \ref setSelected. +*/ + +/* end documentation of signals */ + +/*! + Base class constructor which initializes base class members. +*/ +QCPAbstractItem::QCPAbstractItem(QCustomPlot *parentPlot) : + QCPLayerable(parentPlot), + mClipToAxisRect(false), + mSelectable(true), + mSelected(false) +{ + QList rects = parentPlot->axisRects(); + if (rects.size() > 0) + { + setClipToAxisRect(true); + setClipAxisRect(rects.first()); + } +} + +QCPAbstractItem::~QCPAbstractItem() +{ + // don't delete mPositions because every position is also an anchor and thus in mAnchors + qDeleteAll(mAnchors); +} + +/* can't make this a header inline function, because QPointer breaks with forward declared types, see QTBUG-29588 */ +QCPAxisRect *QCPAbstractItem::clipAxisRect() const +{ + return mClipAxisRect.data(); +} + +/*! + Sets whether the item shall be clipped to an axis rect or whether it shall be visible on the + entire QCustomPlot. The axis rect can be set with \ref setClipAxisRect. + + \see setClipAxisRect +*/ +void QCPAbstractItem::setClipToAxisRect(bool clip) +{ + mClipToAxisRect = clip; + if (mClipToAxisRect) + setParentLayerable(mClipAxisRect.data()); +} + +/*! + Sets the clip axis rect. It defines the rect that will be used to clip the item when \ref + setClipToAxisRect is set to true. + + \see setClipToAxisRect +*/ +void QCPAbstractItem::setClipAxisRect(QCPAxisRect *rect) +{ + mClipAxisRect = rect; + if (mClipToAxisRect) + setParentLayerable(mClipAxisRect.data()); +} + +/*! + Sets whether the user can (de-)select this item by clicking on the QCustomPlot surface. + (When \ref QCustomPlot::setInteractions contains QCustomPlot::iSelectItems.) + + However, even when \a selectable was set to false, it is possible to set the selection manually, + by calling \ref setSelected. + + \see QCustomPlot::setInteractions, setSelected +*/ +void QCPAbstractItem::setSelectable(bool selectable) +{ + if (mSelectable != selectable) + { + mSelectable = selectable; + emit selectableChanged(mSelectable); + } +} + +/*! + Sets whether this item is selected or not. When selected, it might use a different visual + appearance (e.g. pen and brush), this depends on the specific item though. + + The entire selection mechanism for items is handled automatically when \ref + QCustomPlot::setInteractions contains QCustomPlot::iSelectItems. You only need to call this + function when you wish to change the selection state manually. + + This function can change the selection state even when \ref setSelectable was set to false. + + emits the \ref selectionChanged signal when \a selected is different from the previous selection state. + + \see setSelectable, selectTest +*/ +void QCPAbstractItem::setSelected(bool selected) +{ + if (mSelected != selected) + { + mSelected = selected; + emit selectionChanged(mSelected); + } +} + +/*! + Returns the QCPItemPosition with the specified \a name. If this item doesn't have a position by + that name, returns 0. + + This function provides an alternative way to access item positions. Normally, you access + positions direcly by their member pointers (which typically have the same variable name as \a + name). + + \see positions, anchor +*/ +QCPItemPosition *QCPAbstractItem::position(const QString &name) const +{ + for (int i=0; iname() == name) + return mPositions.at(i); + } + qDebug() << Q_FUNC_INFO << "position with name not found:" << name; + return 0; +} + +/*! + Returns the QCPItemAnchor with the specified \a name. If this item doesn't have an anchor by + that name, returns 0. + + This function provides an alternative way to access item anchors. Normally, you access + anchors direcly by their member pointers (which typically have the same variable name as \a + name). + + \see anchors, position +*/ +QCPItemAnchor *QCPAbstractItem::anchor(const QString &name) const +{ + for (int i=0; iname() == name) + return mAnchors.at(i); + } + qDebug() << Q_FUNC_INFO << "anchor with name not found:" << name; + return 0; +} + +/*! + Returns whether this item has an anchor with the specified \a name. + + Note that you can check for positions with this function, too. This is because every position is + also an anchor (QCPItemPosition inherits from QCPItemAnchor). + + \see anchor, position +*/ +bool QCPAbstractItem::hasAnchor(const QString &name) const +{ + for (int i=0; iname() == name) + return true; + } + return false; +} + +/*! \internal + + Returns the rect the visual representation of this item is clipped to. This depends on the + current setting of \ref setClipToAxisRect as well as the axis rect set with \ref setClipAxisRect. + + If the item is not clipped to an axis rect, the \ref QCustomPlot::viewport rect is returned. + + \see draw +*/ +QRect QCPAbstractItem::clipRect() const +{ + if (mClipToAxisRect && mClipAxisRect) + return mClipAxisRect.data()->rect(); + else + return mParentPlot->viewport(); +} + +/*! \internal + + A convenience function to easily set the QPainter::Antialiased hint on the provided \a painter + before drawing item lines. + + This is the antialiasing state the painter passed to the \ref draw method is in by default. + + This function takes into account the local setting of the antialiasing flag as well as the + overrides set with \ref QCustomPlot::setAntialiasedElements and \ref + QCustomPlot::setNotAntialiasedElements. + + \see setAntialiased +*/ +void QCPAbstractItem::applyDefaultAntialiasingHint(QCPPainter *painter) const +{ + applyAntialiasingHint(painter, mAntialiased, QCP::aeItems); +} + +/*! \internal + + Finds the shortest squared distance of \a point to the line segment defined by \a start and \a + end. + + This function may be used to help with the implementation of the \ref selectTest function for + specific items. + + \note This function is identical to QCPAbstractPlottable::distSqrToLine + + \see rectSelectTest +*/ +double QCPAbstractItem::distSqrToLine(const QPointF &start, const QPointF &end, const QPointF &point) const +{ + QVector2D a(start); + QVector2D b(end); + QVector2D p(point); + QVector2D v(b-a); + + double vLengthSqr = v.lengthSquared(); + if (!qFuzzyIsNull(vLengthSqr)) + { + double mu = QVector2D::dotProduct(p-a, v)/vLengthSqr; + if (mu < 0) + return (a-p).lengthSquared(); + else if (mu > 1) + return (b-p).lengthSquared(); + else + return ((a + mu*v)-p).lengthSquared(); + } else + return (a-p).lengthSquared(); +} + +/*! \internal + + A convenience function which returns the selectTest value for a specified \a rect and a specified + click position \a pos. \a filledRect defines whether a click inside the rect should also be + considered a hit or whether only the rect border is sensitive to hits. + + This function may be used to help with the implementation of the \ref selectTest function for + specific items. + + For example, if your item consists of four rects, call this function four times, once for each + rect, in your \ref selectTest reimplementation. Finally, return the minimum of all four returned + values. + + \see distSqrToLine +*/ +double QCPAbstractItem::rectSelectTest(const QRectF &rect, const QPointF &pos, bool filledRect) const +{ + double result = -1; + + // distance to border: + QList lines; + lines << QLineF(rect.topLeft(), rect.topRight()) << QLineF(rect.bottomLeft(), rect.bottomRight()) + << QLineF(rect.topLeft(), rect.bottomLeft()) << QLineF(rect.topRight(), rect.bottomRight()); + double minDistSqr = std::numeric_limits::max(); + for (int i=0; i mParentPlot->selectionTolerance()*0.99) + { + if (rect.contains(pos)) + result = mParentPlot->selectionTolerance()*0.99; + } + return result; +} + +/*! \internal + + Returns the pixel position of the anchor with Id \a anchorId. This function must be reimplemented in + item subclasses if they want to provide anchors (QCPItemAnchor). + + For example, if the item has two anchors with id 0 and 1, this function takes one of these anchor + ids and returns the respective pixel points of the specified anchor. + + \see createAnchor +*/ +QPointF QCPAbstractItem::anchorPixelPoint(int anchorId) const +{ + qDebug() << Q_FUNC_INFO << "called on item which shouldn't have any anchors (this method not reimplemented). anchorId" << anchorId; + return QPointF(); +} + +/*! \internal + + Creates a QCPItemPosition, registers it with this item and returns a pointer to it. The specified + \a name must be a unique string that is usually identical to the variable name of the position + member (This is needed to provide the name-based \ref position access to positions). + + Don't delete positions created by this function manually, as the item will take care of it. + + Use this function in the constructor (initialization list) of the specific item subclass to + create each position member. Don't create QCPItemPositions with \b new yourself, because they + won't be registered with the item properly. + + \see createAnchor +*/ +QCPItemPosition *QCPAbstractItem::createPosition(const QString &name) +{ + if (hasAnchor(name)) + qDebug() << Q_FUNC_INFO << "anchor/position with name exists already:" << name; + QCPItemPosition *newPosition = new QCPItemPosition(mParentPlot, this, name); + mPositions.append(newPosition); + mAnchors.append(newPosition); // every position is also an anchor + newPosition->setAxes(mParentPlot->xAxis, mParentPlot->yAxis); + newPosition->setType(QCPItemPosition::ptPlotCoords); + if (mParentPlot->axisRect()) + newPosition->setAxisRect(mParentPlot->axisRect()); + newPosition->setCoords(0, 0); + return newPosition; +} + +/*! \internal + + Creates a QCPItemAnchor, registers it with this item and returns a pointer to it. The specified + \a name must be a unique string that is usually identical to the variable name of the anchor + member (This is needed to provide the name based \ref anchor access to anchors). + + The \a anchorId must be a number identifying the created anchor. It is recommended to create an + enum (e.g. "AnchorIndex") for this on each item that uses anchors. This id is used by the anchor + to identify itself when it calls QCPAbstractItem::anchorPixelPoint. That function then returns + the correct pixel coordinates for the passed anchor id. + + Don't delete anchors created by this function manually, as the item will take care of it. + + Use this function in the constructor (initialization list) of the specific item subclass to + create each anchor member. Don't create QCPItemAnchors with \b new yourself, because then they + won't be registered with the item properly. + + \see createPosition +*/ +QCPItemAnchor *QCPAbstractItem::createAnchor(const QString &name, int anchorId) +{ + if (hasAnchor(name)) + qDebug() << Q_FUNC_INFO << "anchor/position with name exists already:" << name; + QCPItemAnchor *newAnchor = new QCPItemAnchor(mParentPlot, this, name, anchorId); + mAnchors.append(newAnchor); + return newAnchor; +} + +/* inherits documentation from base class */ +void QCPAbstractItem::selectEvent(QMouseEvent *event, bool additive, const QVariant &details, bool *selectionStateChanged) +{ + Q_UNUSED(event) + Q_UNUSED(details) + if (mSelectable) + { + bool selBefore = mSelected; + setSelected(additive ? !mSelected : true); + if (selectionStateChanged) + *selectionStateChanged = mSelected != selBefore; + } +} + +/* inherits documentation from base class */ +void QCPAbstractItem::deselectEvent(bool *selectionStateChanged) +{ + if (mSelectable) + { + bool selBefore = mSelected; + setSelected(false); + if (selectionStateChanged) + *selectionStateChanged = mSelected != selBefore; + } +} + +/* inherits documentation from base class */ +QCP::Interaction QCPAbstractItem::selectionCategory() const +{ + return QCP::iSelectItems; +} + + +/*! \file */ + + + +//////////////////////////////////////////////////////////////////////////////////////////////////// +//////////////////// QCustomPlot +//////////////////////////////////////////////////////////////////////////////////////////////////// + +/*! \class QCustomPlot + + \brief The central class of the library. This is the QWidget which displays the plot and + interacts with the user. + + For tutorials on how to use QCustomPlot, see the website\n + http://www.qcustomplot.com/ +*/ + +/* start of documentation of inline functions */ + +/*! \fn QRect QCustomPlot::viewport() const + + Returns the viewport rect of this QCustomPlot instance. The viewport is the area the plot is + drawn in, all mechanisms, e.g. margin caluclation take the viewport to be the outer border of the + plot. The viewport normally is the rect() of the QCustomPlot widget, i.e. a rect with top left + (0, 0) and size of the QCustomPlot widget. + + Don't confuse the viewport with the axis rect (QCustomPlot::axisRect). An axis rect is typically + an area enclosed by four axes, where the graphs/plottables are drawn in. The viewport is larger + and contains also the axes themselves, their tick numbers, their labels, the plot title etc. + + Only when saving to a file (see \ref savePng, \ref savePdf etc.) the viewport is temporarily + modified to allow saving plots with sizes independent of the current widget size. +*/ + +/*! \fn QCPLayoutGrid *QCustomPlot::plotLayout() const + + Returns the top level layout of this QCustomPlot instance. It is a \ref QCPLayoutGrid, initially containing just + one cell with the main QCPAxisRect inside. +*/ + +/* end of documentation of inline functions */ +/* start of documentation of signals */ + +/*! \fn void QCustomPlot::mouseDoubleClick(QMouseEvent *event) + + This signal is emitted when the QCustomPlot receives a mouse double click event. +*/ + +/*! \fn void QCustomPlot::mousePress(QMouseEvent *event) + + This signal is emitted when the QCustomPlot receives a mouse press event. + + It is emitted before QCustomPlot handles any other mechanism like range dragging. So a slot + connected to this signal can still influence the behaviour e.g. with \ref QCPAxisRect::setRangeDrag or \ref + QCPAxisRect::setRangeDragAxes. +*/ + +/*! \fn void QCustomPlot::mouseMove(QMouseEvent *event) + + This signal is emitted when the QCustomPlot receives a mouse move event. + + It is emitted before QCustomPlot handles any other mechanism like range dragging. So a slot + connected to this signal can still influence the behaviour e.g. with \ref QCPAxisRect::setRangeDrag or \ref + QCPAxisRect::setRangeDragAxes. + + \warning It is discouraged to change the drag-axes with \ref QCPAxisRect::setRangeDragAxes here, + because the dragging starting point was saved the moment the mouse was pressed. Thus it only has + a meaning for the range drag axes that were set at that moment. If you want to change the drag + axes, consider doing this in the \ref mousePress signal instead. +*/ + +/*! \fn void QCustomPlot::mouseRelease(QMouseEvent *event) + + This signal is emitted when the QCustomPlot receives a mouse release event. + + It is emitted before QCustomPlot handles any other mechanisms like object selection. So a + slot connected to this signal can still influence the behaviour e.g. with \ref setInteractions or + \ref QCPAbstractPlottable::setSelectable. +*/ + +/*! \fn void QCustomPlot::mouseWheel(QMouseEvent *event) + + This signal is emitted when the QCustomPlot receives a mouse wheel event. + + It is emitted before QCustomPlot handles any other mechanisms like range zooming. So a slot + connected to this signal can still influence the behaviour e.g. with \ref QCPAxisRect::setRangeZoom, \ref + QCPAxisRect::setRangeZoomAxes or \ref QCPAxisRect::setRangeZoomFactor. +*/ + +/*! \fn void QCustomPlot::plottableClick(QCPAbstractPlottable *plottable, QMouseEvent *event) + + This signal is emitted when a plottable is clicked. + + \a event is the mouse event that caused the click and \a plottable is the plottable that received + the click. + + \see plottableDoubleClick +*/ + +/*! \fn void QCustomPlot::plottableDoubleClick(QCPAbstractPlottable *plottable, QMouseEvent *event) + + This signal is emitted when a plottable is double clicked. + + \a event is the mouse event that caused the click and \a plottable is the plottable that received + the click. + + \see plottableClick +*/ + +/*! \fn void QCustomPlot::itemClick(QCPAbstractItem *item, QMouseEvent *event) + + This signal is emitted when an item is clicked. + + \a event is the mouse event that caused the click and \a item is the item that received the + click. + + \see itemDoubleClick +*/ + +/*! \fn void QCustomPlot::itemDoubleClick(QCPAbstractItem *item, QMouseEvent *event) + + This signal is emitted when an item is double clicked. + + \a event is the mouse event that caused the click and \a item is the item that received the + click. + + \see itemClick +*/ + +/*! \fn void QCustomPlot::axisClick(QCPAxis *axis, QCPAxis::SelectablePart part, QMouseEvent *event) + + This signal is emitted when an axis is clicked. + + \a event is the mouse event that caused the click, \a axis is the axis that received the click and + \a part indicates the part of the axis that was clicked. + + \see axisDoubleClick +*/ + +/*! \fn void QCustomPlot::axisDoubleClick(QCPAxis *axis, QCPAxis::SelectablePart part, QMouseEvent *event) + + This signal is emitted when an axis is double clicked. + + \a event is the mouse event that caused the click, \a axis is the axis that received the click and + \a part indicates the part of the axis that was clicked. + + \see axisClick +*/ + +/*! \fn void QCustomPlot::legendClick(QCPLegend *legend, QCPAbstractLegendItem *item, QMouseEvent *event) + + This signal is emitted when a legend (item) is clicked. + + \a event is the mouse event that caused the click, \a legend is the legend that received the + click and \a item is the legend item that received the click. If only the legend and no item is + clicked, \a item is 0. This happens for a click inside the legend padding or the space between + two items. + + \see legendDoubleClick +*/ + +/*! \fn void QCustomPlot::legendDoubleClick(QCPLegend *legend, QCPAbstractLegendItem *item, QMouseEvent *event) + + This signal is emitted when a legend (item) is double clicked. + + \a event is the mouse event that caused the click, \a legend is the legend that received the + click and \a item is the legend item that received the click. If only the legend and no item is + clicked, \a item is 0. This happens for a click inside the legend padding or the space between + two items. + + \see legendClick +*/ + +/*! \fn void QCustomPlot:: titleClick(QMouseEvent *event, QCPPlotTitle *title) + + This signal is emitted when a plot title is clicked. + + \a event is the mouse event that caused the click and \a title is the plot title that received + the click. + + \see titleDoubleClick +*/ + +/*! \fn void QCustomPlot::titleDoubleClick(QMouseEvent *event, QCPPlotTitle *title) + + This signal is emitted when a plot title is double clicked. + + \a event is the mouse event that caused the click and \a title is the plot title that received + the click. + + \see titleClick +*/ + +/*! \fn void QCustomPlot::selectionChangedByUser() + + This signal is emitted after the user has changed the selection in the QCustomPlot, e.g. by + clicking. It is not emitted when the selection state of an object has changed programmatically by + a direct call to setSelected() on an object or by calling \ref deselectAll. + + In addition to this signal, selectable objects also provide individual signals, for example + QCPAxis::selectionChanged or QCPAbstractPlottable::selectionChanged. Note that those signals are + emitted even if the selection state is changed programmatically. + + See the documentation of \ref setInteractions for details about the selection mechanism. + + \see selectedPlottables, selectedGraphs, selectedItems, selectedAxes, selectedLegends +*/ + +/*! \fn void QCustomPlot::beforeReplot() + + This signal is emitted immediately before a replot takes place (caused by a call to the slot \ref + replot). + + It is safe to mutually connect the replot slot with this signal on two QCustomPlots to make them + replot synchronously, it won't cause an infinite recursion. + + \see replot, afterReplot +*/ + +/*! \fn void QCustomPlot::afterReplot() + + This signal is emitted immediately after a replot has taken place (caused by a call to the slot \ref + replot). + + It is safe to mutually connect the replot slot with this signal on two QCustomPlots to make them + replot synchronously, it won't cause an infinite recursion. + + \see replot, beforeReplot +*/ + +/* end of documentation of signals */ +/* start of documentation of public members */ + +/*! \var QCPAxis *QCustomPlot::xAxis + + A pointer to the primary x Axis (bottom) of the main axis rect of the plot. + + QCustomPlot offers convenient pointers to the axes (\ref xAxis, \ref yAxis, \ref xAxis2, \ref + yAxis2) and the \ref legend. They make it very easy working with plots that only have a single + axis rect and at most one axis at each axis rect side. If you use \link thelayoutsystem the + layout system\endlink to add multiple axis rects or multiple axes to one side, use the \ref + QCPAxisRect::axis interface to access the new axes. If one of the four default axes or the + default legend is removed due to manipulation of the layout system (e.g. by removing the main + axis rect), the corresponding pointers become 0. +*/ + +/*! \var QCPAxis *QCustomPlot::yAxis + + A pointer to the primary y Axis (left) of the main axis rect of the plot. + + QCustomPlot offers convenient pointers to the axes (\ref xAxis, \ref yAxis, \ref xAxis2, \ref + yAxis2) and the \ref legend. They make it very easy working with plots that only have a single + axis rect and at most one axis at each axis rect side. If you use \link thelayoutsystem the + layout system\endlink to add multiple axis rects or multiple axes to one side, use the \ref + QCPAxisRect::axis interface to access the new axes. If one of the four default axes or the + default legend is removed due to manipulation of the layout system (e.g. by removing the main + axis rect), the corresponding pointers become 0. +*/ + +/*! \var QCPAxis *QCustomPlot::xAxis2 + + A pointer to the secondary x Axis (top) of the main axis rect of the plot. Secondary axes are + invisible by default. Use QCPAxis::setVisible to change this (or use \ref + QCPAxisRect::setupFullAxesBox). + + QCustomPlot offers convenient pointers to the axes (\ref xAxis, \ref yAxis, \ref xAxis2, \ref + yAxis2) and the \ref legend. They make it very easy working with plots that only have a single + axis rect and at most one axis at each axis rect side. If you use \link thelayoutsystem the + layout system\endlink to add multiple axis rects or multiple axes to one side, use the \ref + QCPAxisRect::axis interface to access the new axes. If one of the four default axes or the + default legend is removed due to manipulation of the layout system (e.g. by removing the main + axis rect), the corresponding pointers become 0. +*/ + +/*! \var QCPAxis *QCustomPlot::yAxis2 + + A pointer to the secondary y Axis (right) of the main axis rect of the plot. Secondary axes are + invisible by default. Use QCPAxis::setVisible to change this (or use \ref + QCPAxisRect::setupFullAxesBox). + + QCustomPlot offers convenient pointers to the axes (\ref xAxis, \ref yAxis, \ref xAxis2, \ref + yAxis2) and the \ref legend. They make it very easy working with plots that only have a single + axis rect and at most one axis at each axis rect side. If you use \link thelayoutsystem the + layout system\endlink to add multiple axis rects or multiple axes to one side, use the \ref + QCPAxisRect::axis interface to access the new axes. If one of the four default axes or the + default legend is removed due to manipulation of the layout system (e.g. by removing the main + axis rect), the corresponding pointers become 0. +*/ + +/*! \var QCPLegend *QCustomPlot::legend + + A pointer to the default legend of the main axis rect. The legend is invisible by default. Use + QCPLegend::setVisible to change this. + + QCustomPlot offers convenient pointers to the axes (\ref xAxis, \ref yAxis, \ref xAxis2, \ref + yAxis2) and the \ref legend. They make it very easy working with plots that only have a single + axis rect and at most one axis at each axis rect side. If you use \link thelayoutsystem the + layout system\endlink to add multiple legends to the plot, use the layout system interface to + access the new legend. For example, legends can be placed inside an axis rect's \ref + QCPAxisRect::insetLayout "inset layout", and must then also be accessed via the inset layout. If + the default legend is removed due to manipulation of the layout system (e.g. by removing the main + axis rect), the corresponding pointer becomes 0. +*/ + +/* end of documentation of public members */ + +/*! + Constructs a QCustomPlot and sets reasonable default values. +*/ +QCustomPlot::QCustomPlot(QWidget *parent) : + QWidget(parent), + xAxis(0), + yAxis(0), + xAxis2(0), + yAxis2(0), + legend(0), + mPlotLayout(0), + mAutoAddPlottableToLegend(true), + mAntialiasedElements(QCP::aeNone), + mNotAntialiasedElements(QCP::aeNone), + mInteractions(0), + mSelectionTolerance(8), + mNoAntialiasingOnDrag(false), + mBackgroundBrush(Qt::white, Qt::SolidPattern), + mBackgroundScaled(true), + mBackgroundScaledMode(Qt::KeepAspectRatioByExpanding), + mCurrentLayer(0), + mPlottingHints(QCP::phCacheLabels|QCP::phForceRepaint), + mMultiSelectModifier(Qt::ControlModifier), + mPaintBuffer(size()), + mMouseEventElement(0), + mReplotting(false) +{ + setAttribute(Qt::WA_NoMousePropagation); + setAttribute(Qt::WA_OpaquePaintEvent); + setMouseTracking(true); + QLocale currentLocale = locale(); + currentLocale.setNumberOptions(QLocale::OmitGroupSeparator); + setLocale(currentLocale); + + // create initial layers: + mLayers.append(new QCPLayer(this, QLatin1String("background"))); + mLayers.append(new QCPLayer(this, QLatin1String("grid"))); + mLayers.append(new QCPLayer(this, QLatin1String("main"))); + mLayers.append(new QCPLayer(this, QLatin1String("axes"))); + mLayers.append(new QCPLayer(this, QLatin1String("legend"))); + updateLayerIndices(); + setCurrentLayer(QLatin1String("main")); + + // create initial layout, axis rect and legend: + mPlotLayout = new QCPLayoutGrid; + mPlotLayout->initializeParentPlot(this); + mPlotLayout->setParent(this); // important because if parent is QWidget, QCPLayout::sizeConstraintsChanged will call QWidget::updateGeometry + mPlotLayout->setLayer(QLatin1String("main")); + QCPAxisRect *defaultAxisRect = new QCPAxisRect(this, true); + mPlotLayout->addElement(0, 0, defaultAxisRect); + xAxis = defaultAxisRect->axis(QCPAxis::atBottom); + yAxis = defaultAxisRect->axis(QCPAxis::atLeft); + xAxis2 = defaultAxisRect->axis(QCPAxis::atTop); + yAxis2 = defaultAxisRect->axis(QCPAxis::atRight); + legend = new QCPLegend; + legend->setVisible(false); + defaultAxisRect->insetLayout()->addElement(legend, Qt::AlignRight|Qt::AlignTop); + defaultAxisRect->insetLayout()->setMargins(QMargins(12, 12, 12, 12)); + + defaultAxisRect->setLayer(QLatin1String("background")); + xAxis->setLayer(QLatin1String("axes")); + yAxis->setLayer(QLatin1String("axes")); + xAxis2->setLayer(QLatin1String("axes")); + yAxis2->setLayer(QLatin1String("axes")); + xAxis->grid()->setLayer(QLatin1String("grid")); + yAxis->grid()->setLayer(QLatin1String("grid")); + xAxis2->grid()->setLayer(QLatin1String("grid")); + yAxis2->grid()->setLayer(QLatin1String("grid")); + legend->setLayer(QLatin1String("legend")); + + setViewport(rect()); // needs to be called after mPlotLayout has been created + + replot(); +} + +QCustomPlot::~QCustomPlot() +{ + clearPlottables(); + clearItems(); + + if (mPlotLayout) + { + delete mPlotLayout; + mPlotLayout = 0; + } + + mCurrentLayer = 0; + qDeleteAll(mLayers); // don't use removeLayer, because it would prevent the last layer to be removed + mLayers.clear(); +} + +/*! + Sets which elements are forcibly drawn antialiased as an \a or combination of QCP::AntialiasedElement. + + This overrides the antialiasing settings for whole element groups, normally controlled with the + \a setAntialiasing function on the individual elements. If an element is neither specified in + \ref setAntialiasedElements nor in \ref setNotAntialiasedElements, the antialiasing setting on + each individual element instance is used. + + For example, if \a antialiasedElements contains \ref QCP::aePlottables, all plottables will be + drawn antialiased, no matter what the specific QCPAbstractPlottable::setAntialiased value was set + to. + + if an element in \a antialiasedElements is already set in \ref setNotAntialiasedElements, it is + removed from there. + + \see setNotAntialiasedElements +*/ +void QCustomPlot::setAntialiasedElements(const QCP::AntialiasedElements &antialiasedElements) +{ + mAntialiasedElements = antialiasedElements; + + // make sure elements aren't in mNotAntialiasedElements and mAntialiasedElements simultaneously: + if ((mNotAntialiasedElements & mAntialiasedElements) != 0) + mNotAntialiasedElements |= ~mAntialiasedElements; +} + +/*! + Sets whether the specified \a antialiasedElement is forcibly drawn antialiased. + + See \ref setAntialiasedElements for details. + + \see setNotAntialiasedElement +*/ +void QCustomPlot::setAntialiasedElement(QCP::AntialiasedElement antialiasedElement, bool enabled) +{ + if (!enabled && mAntialiasedElements.testFlag(antialiasedElement)) + mAntialiasedElements &= ~antialiasedElement; + else if (enabled && !mAntialiasedElements.testFlag(antialiasedElement)) + mAntialiasedElements |= antialiasedElement; + + // make sure elements aren't in mNotAntialiasedElements and mAntialiasedElements simultaneously: + if ((mNotAntialiasedElements & mAntialiasedElements) != 0) + mNotAntialiasedElements |= ~mAntialiasedElements; +} + +/*! + Sets which elements are forcibly drawn not antialiased as an \a or combination of + QCP::AntialiasedElement. + + This overrides the antialiasing settings for whole element groups, normally controlled with the + \a setAntialiasing function on the individual elements. If an element is neither specified in + \ref setAntialiasedElements nor in \ref setNotAntialiasedElements, the antialiasing setting on + each individual element instance is used. + + For example, if \a notAntialiasedElements contains \ref QCP::aePlottables, no plottables will be + drawn antialiased, no matter what the specific QCPAbstractPlottable::setAntialiased value was set + to. + + if an element in \a notAntialiasedElements is already set in \ref setAntialiasedElements, it is + removed from there. + + \see setAntialiasedElements +*/ +void QCustomPlot::setNotAntialiasedElements(const QCP::AntialiasedElements ¬AntialiasedElements) +{ + mNotAntialiasedElements = notAntialiasedElements; + + // make sure elements aren't in mNotAntialiasedElements and mAntialiasedElements simultaneously: + if ((mNotAntialiasedElements & mAntialiasedElements) != 0) + mAntialiasedElements |= ~mNotAntialiasedElements; +} + +/*! + Sets whether the specified \a notAntialiasedElement is forcibly drawn not antialiased. + + See \ref setNotAntialiasedElements for details. + + \see setAntialiasedElement +*/ +void QCustomPlot::setNotAntialiasedElement(QCP::AntialiasedElement notAntialiasedElement, bool enabled) +{ + if (!enabled && mNotAntialiasedElements.testFlag(notAntialiasedElement)) + mNotAntialiasedElements &= ~notAntialiasedElement; + else if (enabled && !mNotAntialiasedElements.testFlag(notAntialiasedElement)) + mNotAntialiasedElements |= notAntialiasedElement; + + // make sure elements aren't in mNotAntialiasedElements and mAntialiasedElements simultaneously: + if ((mNotAntialiasedElements & mAntialiasedElements) != 0) + mAntialiasedElements |= ~mNotAntialiasedElements; +} + +/*! + If set to true, adding a plottable (e.g. a graph) to the QCustomPlot automatically also adds the + plottable to the legend (QCustomPlot::legend). + + \see addPlottable, addGraph, QCPLegend::addItem +*/ +void QCustomPlot::setAutoAddPlottableToLegend(bool on) +{ + mAutoAddPlottableToLegend = on; +} + +/*! + Sets the possible interactions of this QCustomPlot as an or-combination of \ref QCP::Interaction + enums. There are the following types of interactions: + + Axis range manipulation is controlled via \ref QCP::iRangeDrag and \ref QCP::iRangeZoom. When the + respective interaction is enabled, the user may drag axes ranges and zoom with the mouse wheel. + For details how to control which axes the user may drag/zoom and in what orientations, see \ref + QCPAxisRect::setRangeDrag, \ref QCPAxisRect::setRangeZoom, \ref QCPAxisRect::setRangeDragAxes, + \ref QCPAxisRect::setRangeZoomAxes. + + Plottable selection is controlled by \ref QCP::iSelectPlottables. If \ref QCP::iSelectPlottables is + set, the user may select plottables (graphs, curves, bars,...) by clicking on them or in their + vicinity (\ref setSelectionTolerance). Whether the user can actually select a plottable can + further be restricted with the \ref QCPAbstractPlottable::setSelectable function on the specific + plottable. To find out whether a specific plottable is selected, call + QCPAbstractPlottable::selected(). To retrieve a list of all currently selected plottables, call + \ref selectedPlottables. If you're only interested in QCPGraphs, you may use the convenience + function \ref selectedGraphs. + + Item selection is controlled by \ref QCP::iSelectItems. If \ref QCP::iSelectItems is set, the user + may select items (QCPItemLine, QCPItemText,...) by clicking on them or in their vicinity. To find + out whether a specific item is selected, call QCPAbstractItem::selected(). To retrieve a list of + all currently selected items, call \ref selectedItems. + + Axis selection is controlled with \ref QCP::iSelectAxes. If \ref QCP::iSelectAxes is set, the user + may select parts of the axes by clicking on them. What parts exactly (e.g. Axis base line, tick + labels, axis label) are selectable can be controlled via \ref QCPAxis::setSelectableParts for + each axis. To retrieve a list of all axes that currently contain selected parts, call \ref + selectedAxes. Which parts of an axis are selected, can be retrieved with QCPAxis::selectedParts(). + + Legend selection is controlled with \ref QCP::iSelectLegend. If this is set, the user may + select the legend itself or individual items by clicking on them. What parts exactly are + selectable can be controlled via \ref QCPLegend::setSelectableParts. To find out whether the + legend or any of its child items are selected, check the value of QCPLegend::selectedParts. To + find out which child items are selected, call \ref QCPLegend::selectedItems. + + All other selectable elements The selection of all other selectable objects (e.g. + QCPPlotTitle, or your own layerable subclasses) is controlled with \ref QCP::iSelectOther. If set, the + user may select those objects by clicking on them. To find out which are currently selected, you + need to check their selected state explicitly. + + If the selection state has changed by user interaction, the \ref selectionChangedByUser signal is + emitted. Each selectable object additionally emits an individual selectionChanged signal whenever + their selection state has changed, i.e. not only by user interaction. + + To allow multiple objects to be selected by holding the selection modifier (\ref + setMultiSelectModifier), set the flag \ref QCP::iMultiSelect. + + \note In addition to the selection mechanism presented here, QCustomPlot always emits + corresponding signals, when an object is clicked or double clicked. see \ref plottableClick and + \ref plottableDoubleClick for example. + + \see setInteraction, setSelectionTolerance +*/ +void QCustomPlot::setInteractions(const QCP::Interactions &interactions) +{ + mInteractions = interactions; +} + +/*! + Sets the single \a interaction of this QCustomPlot to \a enabled. + + For details about the interaction system, see \ref setInteractions. + + \see setInteractions +*/ +void QCustomPlot::setInteraction(const QCP::Interaction &interaction, bool enabled) +{ + if (!enabled && mInteractions.testFlag(interaction)) + mInteractions &= ~interaction; + else if (enabled && !mInteractions.testFlag(interaction)) + mInteractions |= interaction; +} + +/*! + Sets the tolerance that is used to decide whether a click selects an object (e.g. a plottable) or + not. + + If the user clicks in the vicinity of the line of e.g. a QCPGraph, it's only regarded as a + potential selection when the minimum distance between the click position and the graph line is + smaller than \a pixels. Objects that are defined by an area (e.g. QCPBars) only react to clicks + directly inside the area and ignore this selection tolerance. In other words, it only has meaning + for parts of objects that are too thin to exactly hit with a click and thus need such a + tolerance. + + \see setInteractions, QCPLayerable::selectTest +*/ +void QCustomPlot::setSelectionTolerance(int pixels) +{ + mSelectionTolerance = pixels; +} + +/*! + Sets whether antialiasing is disabled for this QCustomPlot while the user is dragging axes + ranges. If many objects, especially plottables, are drawn antialiased, this greatly improves + performance during dragging. Thus it creates a more responsive user experience. As soon as the + user stops dragging, the last replot is done with normal antialiasing, to restore high image + quality. + + \see setAntialiasedElements, setNotAntialiasedElements +*/ +void QCustomPlot::setNoAntialiasingOnDrag(bool enabled) +{ + mNoAntialiasingOnDrag = enabled; +} + +/*! + Sets the plotting hints for this QCustomPlot instance as an \a or combination of QCP::PlottingHint. + + \see setPlottingHint +*/ +void QCustomPlot::setPlottingHints(const QCP::PlottingHints &hints) +{ + mPlottingHints = hints; +} + +/*! + Sets the specified plotting \a hint to \a enabled. + + \see setPlottingHints +*/ +void QCustomPlot::setPlottingHint(QCP::PlottingHint hint, bool enabled) +{ + QCP::PlottingHints newHints = mPlottingHints; + if (!enabled) + newHints &= ~hint; + else + newHints |= hint; + + if (newHints != mPlottingHints) + setPlottingHints(newHints); +} + +/*! + Sets the keyboard modifier that will be recognized as multi-select-modifier. + + If \ref QCP::iMultiSelect is specified in \ref setInteractions, the user may select multiple objects + by clicking on them one after the other while holding down \a modifier. + + By default the multi-select-modifier is set to Qt::ControlModifier. + + \see setInteractions +*/ +void QCustomPlot::setMultiSelectModifier(Qt::KeyboardModifier modifier) +{ + mMultiSelectModifier = modifier; +} + +/*! + Sets the viewport of this QCustomPlot. The Viewport is the area that the top level layout + (QCustomPlot::plotLayout()) uses as its rect. Normally, the viewport is the entire widget rect. + + This function is used to allow arbitrary size exports with \ref toPixmap, \ref savePng, \ref + savePdf, etc. by temporarily changing the viewport size. +*/ +void QCustomPlot::setViewport(const QRect &rect) +{ + mViewport = rect; + if (mPlotLayout) + mPlotLayout->setOuterRect(mViewport); +} + +/*! + Sets \a pm as the viewport background pixmap (see \ref setViewport). The pixmap is always drawn + below all other objects in the plot. + + For cases where the provided pixmap doesn't have the same size as the viewport, scaling can be + enabled with \ref setBackgroundScaled and the scaling mode (whether and how the aspect ratio is + preserved) can be set with \ref setBackgroundScaledMode. To set all these options in one call, + consider using the overloaded version of this function. + + If a background brush was set with \ref setBackground(const QBrush &brush), the viewport will + first be filled with that brush, before drawing the background pixmap. This can be useful for + background pixmaps with translucent areas. + + \see setBackgroundScaled, setBackgroundScaledMode +*/ +void QCustomPlot::setBackground(const QPixmap &pm) +{ + mBackgroundPixmap = pm; + mScaledBackgroundPixmap = QPixmap(); +} + +/*! + Sets the background brush of the viewport (see \ref setViewport). + + Before drawing everything else, the background is filled with \a brush. If a background pixmap + was set with \ref setBackground(const QPixmap &pm), this brush will be used to fill the viewport + before the background pixmap is drawn. This can be useful for background pixmaps with translucent + areas. + + Set \a brush to Qt::NoBrush or Qt::Transparent to leave background transparent. This can be + useful for exporting to image formats which support transparency, e.g. \ref savePng. + + \see setBackgroundScaled, setBackgroundScaledMode +*/ +void QCustomPlot::setBackground(const QBrush &brush) +{ + mBackgroundBrush = brush; +} + +/*! \overload + + Allows setting the background pixmap of the viewport, whether it shall be scaled and how it + shall be scaled in one call. + + \see setBackground(const QPixmap &pm), setBackgroundScaled, setBackgroundScaledMode +*/ +void QCustomPlot::setBackground(const QPixmap &pm, bool scaled, Qt::AspectRatioMode mode) +{ + mBackgroundPixmap = pm; + mScaledBackgroundPixmap = QPixmap(); + mBackgroundScaled = scaled; + mBackgroundScaledMode = mode; +} + +/*! + Sets whether the viewport background pixmap shall be scaled to fit the viewport. If \a scaled is + set to true, control whether and how the aspect ratio of the original pixmap is preserved with + \ref setBackgroundScaledMode. + + Note that the scaled version of the original pixmap is buffered, so there is no performance + penalty on replots. (Except when the viewport dimensions are changed continuously.) + + \see setBackground, setBackgroundScaledMode +*/ +void QCustomPlot::setBackgroundScaled(bool scaled) +{ + mBackgroundScaled = scaled; +} + +/*! + If scaling of the viewport background pixmap is enabled (\ref setBackgroundScaled), use this + function to define whether and how the aspect ratio of the original pixmap is preserved. + + \see setBackground, setBackgroundScaled +*/ +void QCustomPlot::setBackgroundScaledMode(Qt::AspectRatioMode mode) +{ + mBackgroundScaledMode = mode; +} + +/*! + Returns the plottable with \a index. If the index is invalid, returns 0. + + There is an overloaded version of this function with no parameter which returns the last added + plottable, see QCustomPlot::plottable() + + \see plottableCount, addPlottable +*/ +QCPAbstractPlottable *QCustomPlot::plottable(int index) +{ + if (index >= 0 && index < mPlottables.size()) + { + return mPlottables.at(index); + } else + { + qDebug() << Q_FUNC_INFO << "index out of bounds:" << index; + return 0; + } +} + +/*! \overload + + Returns the last plottable that was added with \ref addPlottable. If there are no plottables in + the plot, returns 0. + + \see plottableCount, addPlottable +*/ +QCPAbstractPlottable *QCustomPlot::plottable() +{ + if (!mPlottables.isEmpty()) + { + return mPlottables.last(); + } else + return 0; +} + +/*! + Adds the specified plottable to the plot and, if \ref setAutoAddPlottableToLegend is enabled, to + the legend (QCustomPlot::legend). QCustomPlot takes ownership of the plottable. + + Returns true on success, i.e. when \a plottable isn't already in the plot and the parent plot of + \a plottable is this QCustomPlot (the latter is controlled by what axes were passed in the + plottable's constructor). + + \see plottable, plottableCount, removePlottable, clearPlottables +*/ +bool QCustomPlot::addPlottable(QCPAbstractPlottable *plottable) +{ + if (mPlottables.contains(plottable)) + { + qDebug() << Q_FUNC_INFO << "plottable already added to this QCustomPlot:" << reinterpret_cast(plottable); + return false; + } + if (plottable->parentPlot() != this) + { + qDebug() << Q_FUNC_INFO << "plottable not created with this QCustomPlot as parent:" << reinterpret_cast(plottable); + return false; + } + + mPlottables.append(plottable); + // possibly add plottable to legend: + if (mAutoAddPlottableToLegend) + plottable->addToLegend(); + // special handling for QCPGraphs to maintain the simple graph interface: + if (QCPGraph *graph = qobject_cast(plottable)) + mGraphs.append(graph); + if (!plottable->layer()) // usually the layer is already set in the constructor of the plottable (via QCPLayerable constructor) + plottable->setLayer(currentLayer()); + return true; +} + +/*! + Removes the specified plottable from the plot and, if necessary, from the legend (QCustomPlot::legend). + + Returns true on success. + + \see addPlottable, clearPlottables +*/ +bool QCustomPlot::removePlottable(QCPAbstractPlottable *plottable) +{ + if (!mPlottables.contains(plottable)) + { + qDebug() << Q_FUNC_INFO << "plottable not in list:" << reinterpret_cast(plottable); + return false; + } + + // remove plottable from legend: + plottable->removeFromLegend(); + // special handling for QCPGraphs to maintain the simple graph interface: + if (QCPGraph *graph = qobject_cast(plottable)) + mGraphs.removeOne(graph); + // remove plottable: + delete plottable; + mPlottables.removeOne(plottable); + return true; +} + +/*! \overload + + Removes the plottable by its \a index. +*/ +bool QCustomPlot::removePlottable(int index) +{ + if (index >= 0 && index < mPlottables.size()) + return removePlottable(mPlottables[index]); + else + { + qDebug() << Q_FUNC_INFO << "index out of bounds:" << index; + return false; + } +} + +/*! + Removes all plottables from the plot (and the QCustomPlot::legend, if necessary). + + Returns the number of plottables removed. + + \see removePlottable +*/ +int QCustomPlot::clearPlottables() +{ + int c = mPlottables.size(); + for (int i=c-1; i >= 0; --i) + removePlottable(mPlottables[i]); + return c; +} + +/*! + Returns the number of currently existing plottables in the plot + + \see plottable, addPlottable +*/ +int QCustomPlot::plottableCount() const +{ + return mPlottables.size(); +} + +/*! + Returns a list of the selected plottables. If no plottables are currently selected, the list is empty. + + There is a convenience function if you're only interested in selected graphs, see \ref selectedGraphs. + + \see setInteractions, QCPAbstractPlottable::setSelectable, QCPAbstractPlottable::setSelected +*/ +QList QCustomPlot::selectedPlottables() const +{ + QList result; + foreach (QCPAbstractPlottable *plottable, mPlottables) + { + if (plottable->selected()) + result.append(plottable); + } + return result; +} + +/*! + Returns the plottable at the pixel position \a pos. Plottables that only consist of single lines + (like graphs) have a tolerance band around them, see \ref setSelectionTolerance. If multiple + plottables come into consideration, the one closest to \a pos is returned. + + If \a onlySelectable is true, only plottables that are selectable + (QCPAbstractPlottable::setSelectable) are considered. + + If there is no plottable at \a pos, the return value is 0. + + \see itemAt, layoutElementAt +*/ +QCPAbstractPlottable *QCustomPlot::plottableAt(const QPointF &pos, bool onlySelectable) const +{ + QCPAbstractPlottable *resultPlottable = 0; + double resultDistance = mSelectionTolerance; // only regard clicks with distances smaller than mSelectionTolerance as selections, so initialize with that value + + foreach (QCPAbstractPlottable *plottable, mPlottables) + { + if (onlySelectable && !plottable->selectable()) // we could have also passed onlySelectable to the selectTest function, but checking here is faster, because we have access to QCPabstractPlottable::selectable + continue; + if ((plottable->keyAxis()->axisRect()->rect() & plottable->valueAxis()->axisRect()->rect()).contains(pos.toPoint())) // only consider clicks inside the rect that is spanned by the plottable's key/value axes + { + double currentDistance = plottable->selectTest(pos, false); + if (currentDistance >= 0 && currentDistance < resultDistance) + { + resultPlottable = plottable; + resultDistance = currentDistance; + } + } + } + + return resultPlottable; +} + +/*! + Returns whether this QCustomPlot instance contains the \a plottable. + + \see addPlottable +*/ +bool QCustomPlot::hasPlottable(QCPAbstractPlottable *plottable) const +{ + return mPlottables.contains(plottable); +} + +/*! + Returns the graph with \a index. If the index is invalid, returns 0. + + There is an overloaded version of this function with no parameter which returns the last created + graph, see QCustomPlot::graph() + + \see graphCount, addGraph +*/ +QCPGraph *QCustomPlot::graph(int index) const +{ + if (index >= 0 && index < mGraphs.size()) + { + return mGraphs.at(index); + } else + { + qDebug() << Q_FUNC_INFO << "index out of bounds:" << index; + return 0; + } +} + +/*! \overload + + Returns the last graph, that was created with \ref addGraph. If there are no graphs in the plot, + returns 0. + + \see graphCount, addGraph +*/ +QCPGraph *QCustomPlot::graph() const +{ + if (!mGraphs.isEmpty()) + { + return mGraphs.last(); + } else + return 0; +} + +/*! + Creates a new graph inside the plot. If \a keyAxis and \a valueAxis are left unspecified (0), the + bottom (xAxis) is used as key and the left (yAxis) is used as value axis. If specified, \a + keyAxis and \a valueAxis must reside in this QCustomPlot. + + \a keyAxis will be used as key axis (typically "x") and \a valueAxis as value axis (typically + "y") for the graph. + + Returns a pointer to the newly created graph, or 0 if adding the graph failed. + + \see graph, graphCount, removeGraph, clearGraphs +*/ +QCPGraph *QCustomPlot::addGraph(QCPAxis *keyAxis, QCPAxis *valueAxis) +{ + if (!keyAxis) keyAxis = xAxis; + if (!valueAxis) valueAxis = yAxis; + if (!keyAxis || !valueAxis) + { + qDebug() << Q_FUNC_INFO << "can't use default QCustomPlot xAxis or yAxis, because at least one is invalid (has been deleted)"; + return 0; + } + if (keyAxis->parentPlot() != this || valueAxis->parentPlot() != this) + { + qDebug() << Q_FUNC_INFO << "passed keyAxis or valueAxis doesn't have this QCustomPlot as parent"; + return 0; + } + + QCPGraph *newGraph = new QCPGraph(keyAxis, valueAxis); + if (addPlottable(newGraph)) + { + newGraph->setName(QLatin1String("Graph ")+QString::number(mGraphs.size())); + return newGraph; + } else + { + delete newGraph; + return 0; + } +} + +/*! + Removes the specified \a graph from the plot and, if necessary, from the QCustomPlot::legend. If + any other graphs in the plot have a channel fill set towards the removed graph, the channel fill + property of those graphs is reset to zero (no channel fill). + + Returns true on success. + + \see clearGraphs +*/ +bool QCustomPlot::removeGraph(QCPGraph *graph) +{ + return removePlottable(graph); +} + +/*! \overload + + Removes the graph by its \a index. +*/ +bool QCustomPlot::removeGraph(int index) +{ + if (index >= 0 && index < mGraphs.size()) + return removeGraph(mGraphs[index]); + else + return false; +} + +/*! + Removes all graphs from the plot (and the QCustomPlot::legend, if necessary). + + Returns the number of graphs removed. + + \see removeGraph +*/ +int QCustomPlot::clearGraphs() +{ + int c = mGraphs.size(); + for (int i=c-1; i >= 0; --i) + removeGraph(mGraphs[i]); + return c; +} + +/*! + Returns the number of currently existing graphs in the plot + + \see graph, addGraph +*/ +int QCustomPlot::graphCount() const +{ + return mGraphs.size(); +} + +/*! + Returns a list of the selected graphs. If no graphs are currently selected, the list is empty. + + If you are not only interested in selected graphs but other plottables like QCPCurve, QCPBars, + etc., use \ref selectedPlottables. + + \see setInteractions, selectedPlottables, QCPAbstractPlottable::setSelectable, QCPAbstractPlottable::setSelected +*/ +QList QCustomPlot::selectedGraphs() const +{ + QList result; + foreach (QCPGraph *graph, mGraphs) + { + if (graph->selected()) + result.append(graph); + } + return result; +} + +/*! + Returns the item with \a index. If the index is invalid, returns 0. + + There is an overloaded version of this function with no parameter which returns the last added + item, see QCustomPlot::item() + + \see itemCount, addItem +*/ +QCPAbstractItem *QCustomPlot::item(int index) const +{ + if (index >= 0 && index < mItems.size()) + { + return mItems.at(index); + } else + { + qDebug() << Q_FUNC_INFO << "index out of bounds:" << index; + return 0; + } +} + +/*! \overload + + Returns the last item, that was added with \ref addItem. If there are no items in the plot, + returns 0. + + \see itemCount, addItem +*/ +QCPAbstractItem *QCustomPlot::item() const +{ + if (!mItems.isEmpty()) + { + return mItems.last(); + } else + return 0; +} + +/*! + Adds the specified item to the plot. QCustomPlot takes ownership of the item. + + Returns true on success, i.e. when \a item wasn't already in the plot and the parent plot of \a + item is this QCustomPlot. + + \see item, itemCount, removeItem, clearItems +*/ +bool QCustomPlot::addItem(QCPAbstractItem *item) +{ + if (!mItems.contains(item) && item->parentPlot() == this) + { + mItems.append(item); + return true; + } else + { + qDebug() << Q_FUNC_INFO << "item either already in list or not created with this QCustomPlot as parent:" << reinterpret_cast(item); + return false; + } +} + +/*! + Removes the specified item from the plot. + + Returns true on success. + + \see addItem, clearItems +*/ +bool QCustomPlot::removeItem(QCPAbstractItem *item) +{ + if (mItems.contains(item)) + { + delete item; + mItems.removeOne(item); + return true; + } else + { + qDebug() << Q_FUNC_INFO << "item not in list:" << reinterpret_cast(item); + return false; + } +} + +/*! \overload + + Removes the item by its \a index. +*/ +bool QCustomPlot::removeItem(int index) +{ + if (index >= 0 && index < mItems.size()) + return removeItem(mItems[index]); + else + { + qDebug() << Q_FUNC_INFO << "index out of bounds:" << index; + return false; + } +} + +/*! + Removes all items from the plot. + + Returns the number of items removed. + + \see removeItem +*/ +int QCustomPlot::clearItems() +{ + int c = mItems.size(); + for (int i=c-1; i >= 0; --i) + removeItem(mItems[i]); + return c; +} + +/*! + Returns the number of currently existing items in the plot + + \see item, addItem +*/ +int QCustomPlot::itemCount() const +{ + return mItems.size(); +} + +/*! + Returns a list of the selected items. If no items are currently selected, the list is empty. + + \see setInteractions, QCPAbstractItem::setSelectable, QCPAbstractItem::setSelected +*/ +QList QCustomPlot::selectedItems() const +{ + QList result; + foreach (QCPAbstractItem *item, mItems) + { + if (item->selected()) + result.append(item); + } + return result; +} + +/*! + Returns the item at the pixel position \a pos. Items that only consist of single lines (e.g. \ref + QCPItemLine or \ref QCPItemCurve) have a tolerance band around them, see \ref + setSelectionTolerance. If multiple items come into consideration, the one closest to \a pos is + returned. + + If \a onlySelectable is true, only items that are selectable (QCPAbstractItem::setSelectable) are + considered. + + If there is no item at \a pos, the return value is 0. + + \see plottableAt, layoutElementAt +*/ +QCPAbstractItem *QCustomPlot::itemAt(const QPointF &pos, bool onlySelectable) const +{ + QCPAbstractItem *resultItem = 0; + double resultDistance = mSelectionTolerance; // only regard clicks with distances smaller than mSelectionTolerance as selections, so initialize with that value + + foreach (QCPAbstractItem *item, mItems) + { + if (onlySelectable && !item->selectable()) // we could have also passed onlySelectable to the selectTest function, but checking here is faster, because we have access to QCPAbstractItem::selectable + continue; + if (!item->clipToAxisRect() || item->clipRect().contains(pos.toPoint())) // only consider clicks inside axis cliprect of the item if actually clipped to it + { + double currentDistance = item->selectTest(pos, false); + if (currentDistance >= 0 && currentDistance < resultDistance) + { + resultItem = item; + resultDistance = currentDistance; + } + } + } + + return resultItem; +} + +/*! + Returns whether this QCustomPlot contains the \a item. + + \see addItem +*/ +bool QCustomPlot::hasItem(QCPAbstractItem *item) const +{ + return mItems.contains(item); +} + +/*! + Returns the layer with the specified \a name. If there is no layer with the specified name, 0 is + returned. + + Layer names are case-sensitive. + + \see addLayer, moveLayer, removeLayer +*/ +QCPLayer *QCustomPlot::layer(const QString &name) const +{ + foreach (QCPLayer *layer, mLayers) + { + if (layer->name() == name) + return layer; + } + return 0; +} + +/*! \overload + + Returns the layer by \a index. If the index is invalid, 0 is returned. + + \see addLayer, moveLayer, removeLayer +*/ +QCPLayer *QCustomPlot::layer(int index) const +{ + if (index >= 0 && index < mLayers.size()) + { + return mLayers.at(index); + } else + { + qDebug() << Q_FUNC_INFO << "index out of bounds:" << index; + return 0; + } +} + +/*! + Returns the layer that is set as current layer (see \ref setCurrentLayer). +*/ +QCPLayer *QCustomPlot::currentLayer() const +{ + return mCurrentLayer; +} + +/*! + Sets the layer with the specified \a name to be the current layer. All layerables (\ref + QCPLayerable), e.g. plottables and items, are created on the current layer. + + Returns true on success, i.e. if there is a layer with the specified \a name in the QCustomPlot. + + Layer names are case-sensitive. + + \see addLayer, moveLayer, removeLayer, QCPLayerable::setLayer +*/ +bool QCustomPlot::setCurrentLayer(const QString &name) +{ + if (QCPLayer *newCurrentLayer = layer(name)) + { + return setCurrentLayer(newCurrentLayer); + } else + { + qDebug() << Q_FUNC_INFO << "layer with name doesn't exist:" << name; + return false; + } +} + +/*! \overload + + Sets the provided \a layer to be the current layer. + + Returns true on success, i.e. when \a layer is a valid layer in the QCustomPlot. + + \see addLayer, moveLayer, removeLayer +*/ +bool QCustomPlot::setCurrentLayer(QCPLayer *layer) +{ + if (!mLayers.contains(layer)) + { + qDebug() << Q_FUNC_INFO << "layer not a layer of this QCustomPlot:" << reinterpret_cast(layer); + return false; + } + + mCurrentLayer = layer; + return true; +} + +/*! + Returns the number of currently existing layers in the plot + + \see layer, addLayer +*/ +int QCustomPlot::layerCount() const +{ + return mLayers.size(); +} + +/*! + Adds a new layer to this QCustomPlot instance. The new layer will have the name \a name, which + must be unique. Depending on \a insertMode, it is positioned either below or above \a otherLayer. + + Returns true on success, i.e. if there is no other layer named \a name and \a otherLayer is a + valid layer inside this QCustomPlot. + + If \a otherLayer is 0, the highest layer in the QCustomPlot will be used. + + For an explanation of what layers are in QCustomPlot, see the documentation of \ref QCPLayer. + + \see layer, moveLayer, removeLayer +*/ +bool QCustomPlot::addLayer(const QString &name, QCPLayer *otherLayer, QCustomPlot::LayerInsertMode insertMode) +{ + if (!otherLayer) + otherLayer = mLayers.last(); + if (!mLayers.contains(otherLayer)) + { + qDebug() << Q_FUNC_INFO << "otherLayer not a layer of this QCustomPlot:" << reinterpret_cast(otherLayer); + return false; + } + if (layer(name)) + { + qDebug() << Q_FUNC_INFO << "A layer exists already with the name" << name; + return false; + } + + QCPLayer *newLayer = new QCPLayer(this, name); + mLayers.insert(otherLayer->index() + (insertMode==limAbove ? 1:0), newLayer); + updateLayerIndices(); + return true; +} + +/*! + Removes the specified \a layer and returns true on success. + + All layerables (e.g. plottables and items) on the removed layer will be moved to the layer below + \a layer. If \a layer is the bottom layer, the layerables are moved to the layer above. In both + cases, the total rendering order of all layerables in the QCustomPlot is preserved. + + If \a layer is the current layer (\ref setCurrentLayer), the layer below (or above, if bottom + layer) becomes the new current layer. + + It is not possible to remove the last layer of the plot. + + \see layer, addLayer, moveLayer +*/ +bool QCustomPlot::removeLayer(QCPLayer *layer) +{ + if (!mLayers.contains(layer)) + { + qDebug() << Q_FUNC_INFO << "layer not a layer of this QCustomPlot:" << reinterpret_cast(layer); + return false; + } + if (mLayers.size() < 2) + { + qDebug() << Q_FUNC_INFO << "can't remove last layer"; + return false; + } + + // append all children of this layer to layer below (if this is lowest layer, prepend to layer above) + int removedIndex = layer->index(); + bool isFirstLayer = removedIndex==0; + QCPLayer *targetLayer = isFirstLayer ? mLayers.at(removedIndex+1) : mLayers.at(removedIndex-1); + QList children = layer->children(); + if (isFirstLayer) // prepend in reverse order (so order relative to each other stays the same) + { + for (int i=children.size()-1; i>=0; --i) + children.at(i)->moveToLayer(targetLayer, true); + } else // append normally + { + for (int i=0; imoveToLayer(targetLayer, false); + } + // if removed layer is current layer, change current layer to layer below/above: + if (layer == mCurrentLayer) + setCurrentLayer(targetLayer); + // remove layer: + delete layer; + mLayers.removeOne(layer); + updateLayerIndices(); + return true; +} + +/*! + Moves the specified \a layer either above or below \a otherLayer. Whether it's placed above or + below is controlled with \a insertMode. + + Returns true on success, i.e. when both \a layer and \a otherLayer are valid layers in the + QCustomPlot. + + \see layer, addLayer, moveLayer +*/ +bool QCustomPlot::moveLayer(QCPLayer *layer, QCPLayer *otherLayer, QCustomPlot::LayerInsertMode insertMode) +{ + if (!mLayers.contains(layer)) + { + qDebug() << Q_FUNC_INFO << "layer not a layer of this QCustomPlot:" << reinterpret_cast(layer); + return false; + } + if (!mLayers.contains(otherLayer)) + { + qDebug() << Q_FUNC_INFO << "otherLayer not a layer of this QCustomPlot:" << reinterpret_cast(otherLayer); + return false; + } + + if (layer->index() > otherLayer->index()) + mLayers.move(layer->index(), otherLayer->index() + (insertMode==limAbove ? 1:0)); + else if (layer->index() < otherLayer->index()) + mLayers.move(layer->index(), otherLayer->index() + (insertMode==limAbove ? 0:-1)); + + updateLayerIndices(); + return true; +} + +/*! + Returns the number of axis rects in the plot. + + All axis rects can be accessed via QCustomPlot::axisRect(). + + Initially, only one axis rect exists in the plot. + + \see axisRect, axisRects +*/ +int QCustomPlot::axisRectCount() const +{ + return axisRects().size(); +} + +/*! + Returns the axis rect with \a index. + + Initially, only one axis rect (with index 0) exists in the plot. If multiple axis rects were + added, all of them may be accessed with this function in a linear fashion (even when they are + nested in a layout hierarchy or inside other axis rects via QCPAxisRect::insetLayout). + + \see axisRectCount, axisRects +*/ +QCPAxisRect *QCustomPlot::axisRect(int index) const +{ + const QList rectList = axisRects(); + if (index >= 0 && index < rectList.size()) + { + return rectList.at(index); + } else + { + qDebug() << Q_FUNC_INFO << "invalid axis rect index" << index; + return 0; + } +} + +/*! + Returns all axis rects in the plot. + + \see axisRectCount, axisRect +*/ +QList QCustomPlot::axisRects() const +{ + QList result; + QStack elementStack; + if (mPlotLayout) + elementStack.push(mPlotLayout); + + while (!elementStack.isEmpty()) + { + foreach (QCPLayoutElement *element, elementStack.pop()->elements(false)) + { + if (element) + { + elementStack.push(element); + if (QCPAxisRect *ar = qobject_cast(element)) + result.append(ar); + } + } + } + + return result; +} + +/*! + Returns the layout element at pixel position \a pos. If there is no element at that position, + returns 0. + + Only visible elements are used. If \ref QCPLayoutElement::setVisible on the element itself or on + any of its parent elements is set to false, it will not be considered. + + \see itemAt, plottableAt +*/ +QCPLayoutElement *QCustomPlot::layoutElementAt(const QPointF &pos) const +{ + QCPLayoutElement *currentElement = mPlotLayout; + bool searchSubElements = true; + while (searchSubElements && currentElement) + { + searchSubElements = false; + foreach (QCPLayoutElement *subElement, currentElement->elements(false)) + { + if (subElement && subElement->realVisibility() && subElement->selectTest(pos, false) >= 0) + { + currentElement = subElement; + searchSubElements = true; + break; + } + } + } + return currentElement; +} + +/*! + Returns the axes that currently have selected parts, i.e. whose selection state is not \ref + QCPAxis::spNone. + + \see selectedPlottables, selectedLegends, setInteractions, QCPAxis::setSelectedParts, + QCPAxis::setSelectableParts +*/ +QList QCustomPlot::selectedAxes() const +{ + QList result, allAxes; + foreach (QCPAxisRect *rect, axisRects()) + allAxes << rect->axes(); + + foreach (QCPAxis *axis, allAxes) + { + if (axis->selectedParts() != QCPAxis::spNone) + result.append(axis); + } + + return result; +} + +/*! + Returns the legends that currently have selected parts, i.e. whose selection state is not \ref + QCPLegend::spNone. + + \see selectedPlottables, selectedAxes, setInteractions, QCPLegend::setSelectedParts, + QCPLegend::setSelectableParts, QCPLegend::selectedItems +*/ +QList QCustomPlot::selectedLegends() const +{ + QList result; + + QStack elementStack; + if (mPlotLayout) + elementStack.push(mPlotLayout); + + while (!elementStack.isEmpty()) + { + foreach (QCPLayoutElement *subElement, elementStack.pop()->elements(false)) + { + if (subElement) + { + elementStack.push(subElement); + if (QCPLegend *leg = qobject_cast(subElement)) + { + if (leg->selectedParts() != QCPLegend::spNone) + result.append(leg); + } + } + } + } + + return result; +} + +/*! + Deselects all layerables (plottables, items, axes, legends,...) of the QCustomPlot. + + Since calling this function is not a user interaction, this does not emit the \ref + selectionChangedByUser signal. The individual selectionChanged signals are emitted though, if the + objects were previously selected. + + \see setInteractions, selectedPlottables, selectedItems, selectedAxes, selectedLegends +*/ +void QCustomPlot::deselectAll() +{ + foreach (QCPLayer *layer, mLayers) + { + foreach (QCPLayerable *layerable, layer->children()) + layerable->deselectEvent(0); + } +} + +/*! + Causes a complete replot into the internal buffer. Finally, update() is called, to redraw the + buffer on the QCustomPlot widget surface. This is the method that must be called to make changes, + for example on the axis ranges or data points of graphs, visible. + + Under a few circumstances, QCustomPlot causes a replot by itself. Those are resize events of the + QCustomPlot widget and user interactions (object selection and range dragging/zooming). + + Before the replot happens, the signal \ref beforeReplot is emitted. After the replot, \ref + afterReplot is emitted. It is safe to mutually connect the replot slot with any of those two + signals on two QCustomPlots to make them replot synchronously, it won't cause an infinite + recursion. +*/ +void QCustomPlot::replot(QCustomPlot::RefreshPriority refreshPriority) +{ + if (mReplotting) // incase signals loop back to replot slot + return; + mReplotting = true; + emit beforeReplot(); + + mPaintBuffer.fill(mBackgroundBrush.style() == Qt::SolidPattern ? mBackgroundBrush.color() : Qt::transparent); + QCPPainter painter; + painter.begin(&mPaintBuffer); + if (painter.isActive()) + { + painter.setRenderHint(QPainter::HighQualityAntialiasing); // to make Antialiasing look good if using the OpenGL graphicssystem + if (mBackgroundBrush.style() != Qt::SolidPattern && mBackgroundBrush.style() != Qt::NoBrush) + painter.fillRect(mViewport, mBackgroundBrush); + draw(&painter); + painter.end(); + if ((refreshPriority == rpHint && mPlottingHints.testFlag(QCP::phForceRepaint)) || refreshPriority==rpImmediate) + repaint(); + else + update(); + } else // might happen if QCustomPlot has width or height zero + qDebug() << Q_FUNC_INFO << "Couldn't activate painter on buffer. This usually happens because QCustomPlot has width or height zero."; + + emit afterReplot(); + mReplotting = false; +} + +/*! + Rescales the axes such that all plottables (like graphs) in the plot are fully visible. + + if \a onlyVisiblePlottables is set to true, only the plottables that have their visibility set to true + (QCPLayerable::setVisible), will be used to rescale the axes. + + \see QCPAbstractPlottable::rescaleAxes, QCPAxis::rescale +*/ +void QCustomPlot::rescaleAxes(bool onlyVisiblePlottables) +{ + QList allAxes; + foreach (QCPAxisRect *rect, axisRects()) + allAxes << rect->axes(); + + foreach (QCPAxis *axis, allAxes) + axis->rescale(onlyVisiblePlottables); +} + +/*! + Saves a PDF with the vectorized plot to the file \a fileName. The axis ratio as well as the scale + of texts and lines will be derived from the specified \a width and \a height. This means, the + output will look like the normal on-screen output of a QCustomPlot widget with the corresponding + pixel width and height. If either \a width or \a height is zero, the exported image will have the + same dimensions as the QCustomPlot widget currently has. + + \a noCosmeticPen disables the use of cosmetic pens when drawing to the PDF file. Cosmetic pens + are pens with numerical width 0, which are always drawn as a one pixel wide line, no matter what + zoom factor is set in the PDF-Viewer. For more information about cosmetic pens, see the QPainter + and QPen documentation. + + The objects of the plot will appear in the current selection state. If you don't want any + selected objects to be painted in their selected look, deselect everything with \ref deselectAll + before calling this function. + + Returns true on success. + + \warning + \li If you plan on editing the exported PDF file with a vector graphics editor like + Inkscape, it is advised to set \a noCosmeticPen to true to avoid losing those cosmetic lines + (which might be quite many, because cosmetic pens are the default for e.g. axes and tick marks). + \li If calling this function inside the constructor of the parent of the QCustomPlot widget + (i.e. the MainWindow constructor, if QCustomPlot is inside the MainWindow), always provide + explicit non-zero widths and heights. If you leave \a width or \a height as 0 (default), this + function uses the current width and height of the QCustomPlot widget. However, in Qt, these + aren't defined yet inside the constructor, so you would get an image that has strange + widths/heights. + + \a pdfCreator and \a pdfTitle may be used to set the according metadata fields in the resulting + PDF file. + + \note On Android systems, this method does nothing and issues an according qDebug warning + message. This is also the case if for other reasons the define flag QT_NO_PRINTER is set. + + \see savePng, saveBmp, saveJpg, saveRastered +*/ +bool QCustomPlot::savePdf(const QString &fileName, bool noCosmeticPen, int width, int height, const QString &pdfCreator, const QString &pdfTitle) +{ + bool success = false; +#ifdef QT_NO_PRINTER + Q_UNUSED(fileName) + Q_UNUSED(noCosmeticPen) + Q_UNUSED(width) + Q_UNUSED(height) + Q_UNUSED(pdfCreator) + Q_UNUSED(pdfTitle) + qDebug() << Q_FUNC_INFO << "Qt was built without printer support (QT_NO_PRINTER). PDF not created."; +#else + int newWidth, newHeight; + if (width == 0 || height == 0) + { + newWidth = this->width(); + newHeight = this->height(); + } else + { + newWidth = width; + newHeight = height; + } + + QPrinter printer(QPrinter::ScreenResolution); + printer.setOutputFileName(fileName); + printer.setOutputFormat(QPrinter::PdfFormat); + printer.setColorMode(QPrinter::Color); + printer.printEngine()->setProperty(QPrintEngine::PPK_Creator, pdfCreator); + printer.printEngine()->setProperty(QPrintEngine::PPK_DocumentName, pdfTitle); + QRect oldViewport = viewport(); + setViewport(QRect(0, 0, newWidth, newHeight)); +#if QT_VERSION < QT_VERSION_CHECK(5, 3, 0) + printer.setFullPage(true); + printer.setPaperSize(viewport().size(), QPrinter::DevicePixel); +#else + QPageLayout pageLayout; + pageLayout.setMode(QPageLayout::FullPageMode); + pageLayout.setOrientation(QPageLayout::Portrait); + pageLayout.setMargins(QMarginsF(0, 0, 0, 0)); + pageLayout.setPageSize(QPageSize(viewport().size(), QPageSize::Point, QString(), QPageSize::ExactMatch)); + printer.setPageLayout(pageLayout); +#endif + QCPPainter printpainter; + if (printpainter.begin(&printer)) + { + printpainter.setMode(QCPPainter::pmVectorized); + printpainter.setMode(QCPPainter::pmNoCaching); + printpainter.setMode(QCPPainter::pmNonCosmetic, noCosmeticPen); + printpainter.setWindow(mViewport); + if (mBackgroundBrush.style() != Qt::NoBrush && + mBackgroundBrush.color() != Qt::white && + mBackgroundBrush.color() != Qt::transparent && + mBackgroundBrush.color().alpha() > 0) // draw pdf background color if not white/transparent + printpainter.fillRect(viewport(), mBackgroundBrush); + draw(&printpainter); + printpainter.end(); + success = true; + } + setViewport(oldViewport); +#endif // QT_NO_PRINTER + return success; +} + +/*! + Saves a PNG image file to \a fileName on disc. The output plot will have the dimensions \a width + and \a height in pixels. If either \a width or \a height is zero, the exported image will have + the same dimensions as the QCustomPlot widget currently has. Line widths and texts etc. are not + scaled up when larger widths/heights are used. If you want that effect, use the \a scale parameter. + + For example, if you set both \a width and \a height to 100 and \a scale to 2, you will end up with an + image file of size 200*200 in which all graphical elements are scaled up by factor 2 (line widths, + texts, etc.). This scaling is not done by stretching a 100*100 image, the result will have full + 200*200 pixel resolution. + + If you use a high scaling factor, it is recommended to enable antialiasing for all elements via + temporarily setting \ref QCustomPlot::setAntialiasedElements to \ref QCP::aeAll as this allows + QCustomPlot to place objects with sub-pixel accuracy. + + \warning If calling this function inside the constructor of the parent of the QCustomPlot widget + (i.e. the MainWindow constructor, if QCustomPlot is inside the MainWindow), always provide + explicit non-zero widths and heights. If you leave \a width or \a height as 0 (default), this + function uses the current width and height of the QCustomPlot widget. However, in Qt, these + aren't defined yet inside the constructor, so you would get an image that has strange + widths/heights. + + The objects of the plot will appear in the current selection state. If you don't want any selected + objects to be painted in their selected look, deselect everything with \ref deselectAll before calling + this function. + + If you want the PNG to have a transparent background, call \ref setBackground(const QBrush + &brush) with no brush (Qt::NoBrush) or a transparent color (Qt::transparent), before saving. + + PNG compression can be controlled with the \a quality parameter which must be between 0 and 100 or + -1 to use the default setting. + + Returns true on success. If this function fails, most likely the PNG format isn't supported by + the system, see Qt docs about QImageWriter::supportedImageFormats(). + + \see savePdf, saveBmp, saveJpg, saveRastered +*/ +bool QCustomPlot::savePng(const QString &fileName, int width, int height, double scale, int quality) +{ + return saveRastered(fileName, width, height, scale, "PNG", quality); +} + +/*! + Saves a JPG image file to \a fileName on disc. The output plot will have the dimensions \a width + and \a height in pixels. If either \a width or \a height is zero, the exported image will have + the same dimensions as the QCustomPlot widget currently has. Line widths and texts etc. are not + scaled up when larger widths/heights are used. If you want that effect, use the \a scale parameter. + + For example, if you set both \a width and \a height to 100 and \a scale to 2, you will end up with an + image file of size 200*200 in which all graphical elements are scaled up by factor 2 (line widths, + texts, etc.). This scaling is not done by stretching a 100*100 image, the result will have full + 200*200 pixel resolution. + + If you use a high scaling factor, it is recommended to enable antialiasing for all elements via + temporarily setting \ref QCustomPlot::setAntialiasedElements to \ref QCP::aeAll as this allows + QCustomPlot to place objects with sub-pixel accuracy. + + \warning If calling this function inside the constructor of the parent of the QCustomPlot widget + (i.e. the MainWindow constructor, if QCustomPlot is inside the MainWindow), always provide + explicit non-zero widths and heights. If you leave \a width or \a height as 0 (default), this + function uses the current width and height of the QCustomPlot widget. However, in Qt, these + aren't defined yet inside the constructor, so you would get an image that has strange + widths/heights. + + The objects of the plot will appear in the current selection state. If you don't want any selected + objects to be painted in their selected look, deselect everything with \ref deselectAll before calling + this function. + + JPG compression can be controlled with the \a quality parameter which must be between 0 and 100 or + -1 to use the default setting. + + Returns true on success. If this function fails, most likely the JPG format isn't supported by + the system, see Qt docs about QImageWriter::supportedImageFormats(). + + \see savePdf, savePng, saveBmp, saveRastered +*/ +bool QCustomPlot::saveJpg(const QString &fileName, int width, int height, double scale, int quality) +{ + return saveRastered(fileName, width, height, scale, "JPG", quality); +} + +/*! + Saves a BMP image file to \a fileName on disc. The output plot will have the dimensions \a width + and \a height in pixels. If either \a width or \a height is zero, the exported image will have + the same dimensions as the QCustomPlot widget currently has. Line widths and texts etc. are not + scaled up when larger widths/heights are used. If you want that effect, use the \a scale parameter. + + For example, if you set both \a width and \a height to 100 and \a scale to 2, you will end up with an + image file of size 200*200 in which all graphical elements are scaled up by factor 2 (line widths, + texts, etc.). This scaling is not done by stretching a 100*100 image, the result will have full + 200*200 pixel resolution. + + If you use a high scaling factor, it is recommended to enable antialiasing for all elements via + temporarily setting \ref QCustomPlot::setAntialiasedElements to \ref QCP::aeAll as this allows + QCustomPlot to place objects with sub-pixel accuracy. + + \warning If calling this function inside the constructor of the parent of the QCustomPlot widget + (i.e. the MainWindow constructor, if QCustomPlot is inside the MainWindow), always provide + explicit non-zero widths and heights. If you leave \a width or \a height as 0 (default), this + function uses the current width and height of the QCustomPlot widget. However, in Qt, these + aren't defined yet inside the constructor, so you would get an image that has strange + widths/heights. + + The objects of the plot will appear in the current selection state. If you don't want any selected + objects to be painted in their selected look, deselect everything with \ref deselectAll before calling + this function. + + Returns true on success. If this function fails, most likely the BMP format isn't supported by + the system, see Qt docs about QImageWriter::supportedImageFormats(). + + \see savePdf, savePng, saveJpg, saveRastered +*/ +bool QCustomPlot::saveBmp(const QString &fileName, int width, int height, double scale) +{ + return saveRastered(fileName, width, height, scale, "BMP"); +} + +/*! \internal + + Returns a minimum size hint that corresponds to the minimum size of the top level layout + (\ref plotLayout). To prevent QCustomPlot from being collapsed to size/width zero, set a minimum + size (setMinimumSize) either on the whole QCustomPlot or on any layout elements inside the plot. + This is especially important, when placed in a QLayout where other components try to take in as + much space as possible (e.g. QMdiArea). +*/ +QSize QCustomPlot::minimumSizeHint() const +{ + return mPlotLayout->minimumSizeHint(); +} + +/*! \internal + + Returns a size hint that is the same as \ref minimumSizeHint. + +*/ +QSize QCustomPlot::sizeHint() const +{ + return mPlotLayout->minimumSizeHint(); +} + +/*! \internal + + Event handler for when the QCustomPlot widget needs repainting. This does not cause a \ref replot, but + draws the internal buffer on the widget surface. +*/ +void QCustomPlot::paintEvent(QPaintEvent *event) +{ + Q_UNUSED(event); + QPainter painter(this); + painter.drawPixmap(0, 0, mPaintBuffer); +} + +/*! \internal + + Event handler for a resize of the QCustomPlot widget. Causes the internal buffer to be resized to + the new size. The viewport (which becomes the outer rect of mPlotLayout) is resized + appropriately. Finally a \ref replot is performed. +*/ +void QCustomPlot::resizeEvent(QResizeEvent *event) +{ + // resize and repaint the buffer: + mPaintBuffer = QPixmap(event->size()); + setViewport(rect()); + replot(rpQueued); // queued update is important here, to prevent painting issues in some contexts +} + +/*! \internal + + Event handler for when a double click occurs. Emits the \ref mouseDoubleClick signal, then emits + the specialized signals when certain objecs are clicked (e.g. \ref plottableDoubleClick, \ref + axisDoubleClick, etc.). Finally determines the affected layout element and forwards the event to + it. + + \see mousePressEvent, mouseReleaseEvent +*/ +void QCustomPlot::mouseDoubleClickEvent(QMouseEvent *event) +{ + emit mouseDoubleClick(event); + + QVariant details; + QCPLayerable *clickedLayerable = layerableAt(event->pos(), false, &details); + + // emit specialized object double click signals: + if (QCPAbstractPlottable *ap = qobject_cast(clickedLayerable)) + emit plottableDoubleClick(ap, event); + else if (QCPAxis *ax = qobject_cast(clickedLayerable)) + emit axisDoubleClick(ax, details.value(), event); + else if (QCPAbstractItem *ai = qobject_cast(clickedLayerable)) + emit itemDoubleClick(ai, event); + else if (QCPLegend *lg = qobject_cast(clickedLayerable)) + emit legendDoubleClick(lg, 0, event); + else if (QCPAbstractLegendItem *li = qobject_cast(clickedLayerable)) + emit legendDoubleClick(li->parentLegend(), li, event); + else if (QCPPlotTitle *pt = qobject_cast(clickedLayerable)) + emit titleDoubleClick(event, pt); + + // call double click event of affected layout element: + if (QCPLayoutElement *el = layoutElementAt(event->pos())) + el->mouseDoubleClickEvent(event); + + // call release event of affected layout element (as in mouseReleaseEvent, since the mouseDoubleClick replaces the second release event in double click case): + if (mMouseEventElement) + { + mMouseEventElement->mouseReleaseEvent(event); + mMouseEventElement = 0; + } + + //QWidget::mouseDoubleClickEvent(event); don't call base class implementation because it would just cause a mousePress/ReleaseEvent, which we don't want. +} + +/*! \internal + + Event handler for when a mouse button is pressed. Emits the mousePress signal. Then determines + the affected layout element and forwards the event to it. + + \see mouseMoveEvent, mouseReleaseEvent +*/ +void QCustomPlot::mousePressEvent(QMouseEvent *event) +{ + emit mousePress(event); + mMousePressPos = event->pos(); // need this to determine in releaseEvent whether it was a click (no position change between press and release) + + // call event of affected layout element: + mMouseEventElement = layoutElementAt(event->pos()); + if (mMouseEventElement) + mMouseEventElement->mousePressEvent(event); + + QWidget::mousePressEvent(event); +} + +/*! \internal + + Event handler for when the cursor is moved. Emits the \ref mouseMove signal. + + If a layout element has mouse capture focus (a mousePressEvent happened on top of the layout + element before), the mouseMoveEvent is forwarded to that element. + + \see mousePressEvent, mouseReleaseEvent +*/ +void QCustomPlot::mouseMoveEvent(QMouseEvent *event) +{ + emit mouseMove(event); + + // call event of affected layout element: + if (mMouseEventElement) + mMouseEventElement->mouseMoveEvent(event); + + QWidget::mouseMoveEvent(event); +} + +/*! \internal + + Event handler for when a mouse button is released. Emits the \ref mouseRelease signal. + + If the mouse was moved less than a certain threshold in any direction since the \ref + mousePressEvent, it is considered a click which causes the selection mechanism (if activated via + \ref setInteractions) to possibly change selection states accordingly. Further, specialized mouse + click signals are emitted (e.g. \ref plottableClick, \ref axisClick, etc.) + + If a layout element has mouse capture focus (a \ref mousePressEvent happened on top of the layout + element before), the \ref mouseReleaseEvent is forwarded to that element. + + \see mousePressEvent, mouseMoveEvent +*/ +void QCustomPlot::mouseReleaseEvent(QMouseEvent *event) +{ + emit mouseRelease(event); + bool doReplot = false; + + if ((mMousePressPos-event->pos()).manhattanLength() < 5) // determine whether it was a click operation + { + if (event->button() == Qt::LeftButton) + { + // handle selection mechanism: + QVariant details; + QCPLayerable *clickedLayerable = layerableAt(event->pos(), true, &details); + bool selectionStateChanged = false; + bool additive = mInteractions.testFlag(QCP::iMultiSelect) && event->modifiers().testFlag(mMultiSelectModifier); + // deselect all other layerables if not additive selection: + if (!additive) + { + foreach (QCPLayer *layer, mLayers) + { + foreach (QCPLayerable *layerable, layer->children()) + { + if (layerable != clickedLayerable && mInteractions.testFlag(layerable->selectionCategory())) + { + bool selChanged = false; + layerable->deselectEvent(&selChanged); + selectionStateChanged |= selChanged; + } + } + } + } + if (clickedLayerable && mInteractions.testFlag(clickedLayerable->selectionCategory())) + { + // a layerable was actually clicked, call its selectEvent: + bool selChanged = false; + clickedLayerable->selectEvent(event, additive, details, &selChanged); + selectionStateChanged |= selChanged; + } + if (selectionStateChanged) + { + doReplot = true; + emit selectionChangedByUser(); + } + } + + // emit specialized object click signals: + QVariant details; + QCPLayerable *clickedLayerable = layerableAt(event->pos(), false, &details); // for these signals, selectability is ignored, that's why we call this again with onlySelectable set to false + if (QCPAbstractPlottable *ap = qobject_cast(clickedLayerable)) + emit plottableClick(ap, event); + else if (QCPAxis *ax = qobject_cast(clickedLayerable)) + emit axisClick(ax, details.value(), event); + else if (QCPAbstractItem *ai = qobject_cast(clickedLayerable)) + emit itemClick(ai, event); + else if (QCPLegend *lg = qobject_cast(clickedLayerable)) + emit legendClick(lg, 0, event); + else if (QCPAbstractLegendItem *li = qobject_cast(clickedLayerable)) + emit legendClick(li->parentLegend(), li, event); + else if (QCPPlotTitle *pt = qobject_cast(clickedLayerable)) + emit titleClick(event, pt); + } + + // call event of affected layout element: + if (mMouseEventElement) + { + mMouseEventElement->mouseReleaseEvent(event); + mMouseEventElement = 0; + } + + if (doReplot || noAntialiasingOnDrag()) + replot(); + + QWidget::mouseReleaseEvent(event); +} + +/*! \internal + + Event handler for mouse wheel events. First, the \ref mouseWheel signal is emitted. Then + determines the affected layout element and forwards the event to it. + +*/ +void QCustomPlot::wheelEvent(QWheelEvent *event) +{ + emit mouseWheel(event); + + // call event of affected layout element: + if (QCPLayoutElement *el = layoutElementAt(event->pos())) + el->wheelEvent(event); + + QWidget::wheelEvent(event); +} + +/*! \internal + + This is the main draw function. It draws the entire plot, including background pixmap, with the + specified \a painter. Note that it does not fill the background with the background brush (as the + user may specify with \ref setBackground(const QBrush &brush)), this is up to the respective + functions calling this method (e.g. \ref replot, \ref toPixmap and \ref toPainter). +*/ +void QCustomPlot::draw(QCPPainter *painter) +{ + // run through layout phases: + mPlotLayout->update(QCPLayoutElement::upPreparation); + mPlotLayout->update(QCPLayoutElement::upMargins); + mPlotLayout->update(QCPLayoutElement::upLayout); + + // draw viewport background pixmap: + drawBackground(painter); + + // draw all layered objects (grid, axes, plottables, items, legend,...): + foreach (QCPLayer *layer, mLayers) + { + foreach (QCPLayerable *child, layer->children()) + { + if (child->realVisibility()) + { + painter->save(); + painter->setClipRect(child->clipRect().translated(0, -1)); + child->applyDefaultAntialiasingHint(painter); + child->draw(painter); + painter->restore(); + } + } + } + + /* Debug code to draw all layout element rects + foreach (QCPLayoutElement* el, findChildren()) + { + painter->setBrush(Qt::NoBrush); + painter->setPen(QPen(QColor(0, 0, 0, 100), 0, Qt::DashLine)); + painter->drawRect(el->rect()); + painter->setPen(QPen(QColor(255, 0, 0, 100), 0, Qt::DashLine)); + painter->drawRect(el->outerRect()); + } + */ +} + +/*! \internal + + Draws the viewport background pixmap of the plot. + + If a pixmap was provided via \ref setBackground, this function buffers the scaled version + depending on \ref setBackgroundScaled and \ref setBackgroundScaledMode and then draws it inside + the viewport with the provided \a painter. The scaled version is buffered in + mScaledBackgroundPixmap to prevent expensive rescaling at every redraw. It is only updated, when + the axis rect has changed in a way that requires a rescale of the background pixmap (this is + dependent on the \ref setBackgroundScaledMode), or when a differend axis background pixmap was + set. + + Note that this function does not draw a fill with the background brush (\ref setBackground(const + QBrush &brush)) beneath the pixmap. + + \see setBackground, setBackgroundScaled, setBackgroundScaledMode +*/ +void QCustomPlot::drawBackground(QCPPainter *painter) +{ + // Note: background color is handled in individual replot/save functions + + // draw background pixmap (on top of fill, if brush specified): + if (!mBackgroundPixmap.isNull()) + { + if (mBackgroundScaled) + { + // check whether mScaledBackground needs to be updated: + QSize scaledSize(mBackgroundPixmap.size()); + scaledSize.scale(mViewport.size(), mBackgroundScaledMode); + if (mScaledBackgroundPixmap.size() != scaledSize) + mScaledBackgroundPixmap = mBackgroundPixmap.scaled(mViewport.size(), mBackgroundScaledMode, Qt::SmoothTransformation); + painter->drawPixmap(mViewport.topLeft(), mScaledBackgroundPixmap, QRect(0, 0, mViewport.width(), mViewport.height()) & mScaledBackgroundPixmap.rect()); + } else + { + painter->drawPixmap(mViewport.topLeft(), mBackgroundPixmap, QRect(0, 0, mViewport.width(), mViewport.height())); + } + } +} + + +/*! \internal + + This method is used by \ref QCPAxisRect::removeAxis to report removed axes to the QCustomPlot + so it may clear its QCustomPlot::xAxis, yAxis, xAxis2 and yAxis2 members accordingly. +*/ +void QCustomPlot::axisRemoved(QCPAxis *axis) +{ + if (xAxis == axis) + xAxis = 0; + if (xAxis2 == axis) + xAxis2 = 0; + if (yAxis == axis) + yAxis = 0; + if (yAxis2 == axis) + yAxis2 = 0; + + // Note: No need to take care of range drag axes and range zoom axes, because they are stored in smart pointers +} + +/*! \internal + + This method is used by the QCPLegend destructor to report legend removal to the QCustomPlot so + it may clear its QCustomPlot::legend member accordingly. +*/ +void QCustomPlot::legendRemoved(QCPLegend *legend) +{ + if (this->legend == legend) + this->legend = 0; +} + +/*! \internal + + Assigns all layers their index (QCPLayer::mIndex) in the mLayers list. This method is thus called + after every operation that changes the layer indices, like layer removal, layer creation, layer + moving. +*/ +void QCustomPlot::updateLayerIndices() const +{ + for (int i=0; imIndex = i; +} + +/*! \internal + + Returns the layerable at pixel position \a pos. If \a onlySelectable is set to true, only those + layerables that are selectable will be considered. (Layerable subclasses communicate their + selectability via the QCPLayerable::selectTest method, by returning -1.) + + \a selectionDetails is an output parameter that contains selection specifics of the affected + layerable. This is useful if the respective layerable shall be given a subsequent + QCPLayerable::selectEvent (like in \ref mouseReleaseEvent). \a selectionDetails usually contains + information about which part of the layerable was hit, in multi-part layerables (e.g. + QCPAxis::SelectablePart). +*/ +QCPLayerable *QCustomPlot::layerableAt(const QPointF &pos, bool onlySelectable, QVariant *selectionDetails) const +{ + for (int layerIndex=mLayers.size()-1; layerIndex>=0; --layerIndex) + { + const QList layerables = mLayers.at(layerIndex)->children(); + double minimumDistance = selectionTolerance()*1.1; + QCPLayerable *minimumDistanceLayerable = 0; + for (int i=layerables.size()-1; i>=0; --i) + { + if (!layerables.at(i)->realVisibility()) + continue; + QVariant details; + double dist = layerables.at(i)->selectTest(pos, onlySelectable, &details); + if (dist >= 0 && dist < minimumDistance) + { + minimumDistance = dist; + minimumDistanceLayerable = layerables.at(i); + if (selectionDetails) *selectionDetails = details; + } + } + if (minimumDistance < selectionTolerance()) + return minimumDistanceLayerable; + } + return 0; +} + +/*! + Saves the plot to a rastered image file \a fileName in the image format \a format. The plot is + sized to \a width and \a height in pixels and scaled with \a scale. (width 100 and scale 2.0 lead + to a full resolution file with width 200.) If the \a format supports compression, \a quality may + be between 0 and 100 to control it. + + Returns true on success. If this function fails, most likely the given \a format isn't supported + by the system, see Qt docs about QImageWriter::supportedImageFormats(). + + \see saveBmp, saveJpg, savePng, savePdf +*/ +bool QCustomPlot::saveRastered(const QString &fileName, int width, int height, double scale, const char *format, int quality) +{ + QPixmap buffer = toPixmap(width, height, scale); + if (!buffer.isNull()) + return buffer.save(fileName, format, quality); + else + return false; +} + +/*! + Renders the plot to a pixmap and returns it. + + The plot is sized to \a width and \a height in pixels and scaled with \a scale. (width 100 and + scale 2.0 lead to a full resolution pixmap with width 200.) + + \see toPainter, saveRastered, saveBmp, savePng, saveJpg, savePdf +*/ +QPixmap QCustomPlot::toPixmap(int width, int height, double scale) +{ + // this method is somewhat similar to toPainter. Change something here, and a change in toPainter might be necessary, too. + int newWidth, newHeight; + if (width == 0 || height == 0) + { + newWidth = this->width(); + newHeight = this->height(); + } else + { + newWidth = width; + newHeight = height; + } + int scaledWidth = qRound(scale*newWidth); + int scaledHeight = qRound(scale*newHeight); + + QPixmap result(scaledWidth, scaledHeight); + result.fill(mBackgroundBrush.style() == Qt::SolidPattern ? mBackgroundBrush.color() : Qt::transparent); // if using non-solid pattern, make transparent now and draw brush pattern later + QCPPainter painter; + painter.begin(&result); + if (painter.isActive()) + { + QRect oldViewport = viewport(); + setViewport(QRect(0, 0, newWidth, newHeight)); + painter.setMode(QCPPainter::pmNoCaching); + if (!qFuzzyCompare(scale, 1.0)) + { + if (scale > 1.0) // for scale < 1 we always want cosmetic pens where possible, because else lines might disappear for very small scales + painter.setMode(QCPPainter::pmNonCosmetic); + painter.scale(scale, scale); + } + if (mBackgroundBrush.style() != Qt::SolidPattern && mBackgroundBrush.style() != Qt::NoBrush) // solid fills were done a few lines above with QPixmap::fill + painter.fillRect(mViewport, mBackgroundBrush); + draw(&painter); + setViewport(oldViewport); + painter.end(); + } else // might happen if pixmap has width or height zero + { + qDebug() << Q_FUNC_INFO << "Couldn't activate painter on pixmap"; + return QPixmap(); + } + return result; +} + +/*! + Renders the plot using the passed \a painter. + + The plot is sized to \a width and \a height in pixels. If the \a painter's scale is not 1.0, the resulting plot will + appear scaled accordingly. + + \note If you are restricted to using a QPainter (instead of QCPPainter), create a temporary QPicture and open a QCPPainter + on it. Then call \ref toPainter with this QCPPainter. After ending the paint operation on the picture, draw it with + the QPainter. This will reproduce the painter actions the QCPPainter took, with a QPainter. + + \see toPixmap +*/ +void QCustomPlot::toPainter(QCPPainter *painter, int width, int height) +{ + // this method is somewhat similar to toPixmap. Change something here, and a change in toPixmap might be necessary, too. + int newWidth, newHeight; + if (width == 0 || height == 0) + { + newWidth = this->width(); + newHeight = this->height(); + } else + { + newWidth = width; + newHeight = height; + } + + if (painter->isActive()) + { + QRect oldViewport = viewport(); + setViewport(QRect(0, 0, newWidth, newHeight)); + painter->setMode(QCPPainter::pmNoCaching); + if (mBackgroundBrush.style() != Qt::NoBrush) // unlike in toPixmap, we can't do QPixmap::fill for Qt::SolidPattern brush style, so we also draw solid fills with fillRect here + painter->fillRect(mViewport, mBackgroundBrush); + draw(painter); + setViewport(oldViewport); + } else + qDebug() << Q_FUNC_INFO << "Passed painter is not active"; +} + + +//////////////////////////////////////////////////////////////////////////////////////////////////// +//////////////////// QCPColorGradient +//////////////////////////////////////////////////////////////////////////////////////////////////// + +/*! \class QCPColorGradient + \brief Defines a color gradient for use with e.g. \ref QCPColorMap + + This class describes a color gradient which can be used to encode data with color. For example, + QCPColorMap and QCPColorScale have \ref QCPColorMap::setGradient "setGradient" methods which + take an instance of this class. Colors are set with \ref setColorStopAt(double position, const QColor &color) + with a \a position from 0 to 1. In between these defined color positions, the + color will be interpolated linearly either in RGB or HSV space, see \ref setColorInterpolation. + + Alternatively, load one of the preset color gradients shown in the image below, with \ref + loadPreset, or by directly specifying the preset in the constructor. + + \image html QCPColorGradient.png + + The fact that the \ref QCPColorGradient(GradientPreset preset) constructor allows directly + converting a \ref GradientPreset to a QCPColorGradient, you can also directly pass \ref + GradientPreset to all the \a setGradient methods, e.g.: + \snippet documentation/doc-code-snippets/mainwindow.cpp qcpcolorgradient-setgradient + + The total number of levels used in the gradient can be set with \ref setLevelCount. Whether the + color gradient shall be applied periodically (wrapping around) to data values that lie outside + the data range specified on the plottable instance can be controlled with \ref setPeriodic. +*/ + +/*! + Constructs a new QCPColorGradient initialized with the colors and color interpolation according + to \a preset. + + The color level count is initialized to 350. +*/ +QCPColorGradient::QCPColorGradient(GradientPreset preset) : + mLevelCount(350), + mColorInterpolation(ciRGB), + mPeriodic(false), + mColorBufferInvalidated(true) +{ + mColorBuffer.fill(qRgb(0, 0, 0), mLevelCount); + loadPreset(preset); +} + +/* undocumented operator */ +bool QCPColorGradient::operator==(const QCPColorGradient &other) const +{ + return ((other.mLevelCount == this->mLevelCount) && + (other.mColorInterpolation == this->mColorInterpolation) && + (other.mPeriodic == this->mPeriodic) && + (other.mColorStops == this->mColorStops)); +} + +/*! + Sets the number of discretization levels of the color gradient to \a n. The default is 350 which + is typically enough to create a smooth appearance. + + \image html QCPColorGradient-levelcount.png +*/ +void QCPColorGradient::setLevelCount(int n) +{ + if (n < 2) + { + qDebug() << Q_FUNC_INFO << "n must be greater or equal 2 but was" << n; + n = 2; + } + if (n != mLevelCount) + { + mLevelCount = n; + mColorBufferInvalidated = true; + } +} + +/*! + Sets at which positions from 0 to 1 which color shall occur. The positions are the keys, the + colors are the values of the passed QMap \a colorStops. In between these color stops, the color + is interpolated according to \ref setColorInterpolation. + + A more convenient way to create a custom gradient may be to clear all color stops with \ref + clearColorStops and then adding them one by one with \ref setColorStopAt. + + \see clearColorStops +*/ +void QCPColorGradient::setColorStops(const QMap &colorStops) +{ + mColorStops = colorStops; + mColorBufferInvalidated = true; +} + +/*! + Sets the \a color the gradient will have at the specified \a position (from 0 to 1). In between + these color stops, the color is interpolated according to \ref setColorInterpolation. + + \see setColorStops, clearColorStops +*/ +void QCPColorGradient::setColorStopAt(double position, const QColor &color) +{ + mColorStops.insert(position, color); + mColorBufferInvalidated = true; +} + +/*! + Sets whether the colors in between the configured color stops (see \ref setColorStopAt) shall be + interpolated linearly in RGB or in HSV color space. + + For example, a sweep in RGB space from red to green will have a muddy brown intermediate color, + whereas in HSV space the intermediate color is yellow. +*/ +void QCPColorGradient::setColorInterpolation(QCPColorGradient::ColorInterpolation interpolation) +{ + if (interpolation != mColorInterpolation) + { + mColorInterpolation = interpolation; + mColorBufferInvalidated = true; + } +} + +/*! + Sets whether data points that are outside the configured data range (e.g. \ref + QCPColorMap::setDataRange) are colored by periodically repeating the color gradient or whether + they all have the same color, corresponding to the respective gradient boundary color. + + \image html QCPColorGradient-periodic.png + + As shown in the image above, gradients that have the same start and end color are especially + suitable for a periodic gradient mapping, since they produce smooth color transitions throughout + the color map. A preset that has this property is \ref gpHues. + + In practice, using periodic color gradients makes sense when the data corresponds to a periodic + dimension, such as an angle or a phase. If this is not the case, the color encoding might become + ambiguous, because multiple different data values are shown as the same color. +*/ +void QCPColorGradient::setPeriodic(bool enabled) +{ + mPeriodic = enabled; +} + +/*! + This method is used to quickly convert a \a data array to colors. The colors will be output in + the array \a scanLine. Both \a data and \a scanLine must have the length \a n when passed to this + function. The data range that shall be used for mapping the data value to the gradient is passed + in \a range. \a logarithmic indicates whether the data values shall be mapped to colors + logarithmically. + + if \a data actually contains 2D-data linearized via [row*columnCount + column], you can + set \a dataIndexFactor to columnCount to convert a column instead of a row of the data + array, in \a scanLine. \a scanLine will remain a regular (1D) array. This works because \a data + is addressed data[i*dataIndexFactor]. +*/ +void QCPColorGradient::colorize(const double *data, const QCPRange &range, QRgb *scanLine, int n, int dataIndexFactor, bool logarithmic) +{ + // If you change something here, make sure to also adapt ::color() + if (!data) + { + qDebug() << Q_FUNC_INFO << "null pointer given as data"; + return; + } + if (!scanLine) + { + qDebug() << Q_FUNC_INFO << "null pointer given as scanLine"; + return; + } + if (mColorBufferInvalidated) + updateColorBuffer(); + + if (!logarithmic) + { + const double posToIndexFactor = (mLevelCount-1)/range.size(); + if (mPeriodic) + { + for (int i=0; i= mLevelCount) + index = mLevelCount-1; + scanLine[i] = mColorBuffer.at(index); + } + } + } else // logarithmic == true + { + if (mPeriodic) + { + for (int i=0; i= mLevelCount) + index = mLevelCount-1; + scanLine[i] = mColorBuffer.at(index); + } + } + } +} + +/*! \internal + + This method is used to colorize a single data value given in \a position, to colors. The data + range that shall be used for mapping the data value to the gradient is passed in \a range. \a + logarithmic indicates whether the data value shall be mapped to a color logarithmically. + + If an entire array of data values shall be converted, rather use \ref colorize, for better + performance. +*/ +QRgb QCPColorGradient::color(double position, const QCPRange &range, bool logarithmic) +{ + // If you change something here, make sure to also adapt ::colorize() + if (mColorBufferInvalidated) + updateColorBuffer(); + int index = 0; + if (!logarithmic) + index = (position-range.lower)*(mLevelCount-1)/range.size(); + else + index = qLn(position/range.lower)/qLn(range.upper/range.lower)*(mLevelCount-1); + if (mPeriodic) + { + index = index % mLevelCount; + if (index < 0) + index += mLevelCount; + } else + { + if (index < 0) + index = 0; + else if (index >= mLevelCount) + index = mLevelCount-1; + } + return mColorBuffer.at(index); +} + +/*! + Clears the current color stops and loads the specified \a preset. A preset consists of predefined + color stops and the corresponding color interpolation method. + + The available presets are: + \image html QCPColorGradient.png +*/ +void QCPColorGradient::loadPreset(GradientPreset preset) +{ + clearColorStops(); + switch (preset) + { + case gpGrayscale: + setColorInterpolation(ciRGB); + setColorStopAt(0, Qt::black); + setColorStopAt(1, Qt::white); + break; + case gpHot: + setColorInterpolation(ciRGB); + setColorStopAt(0, QColor(50, 0, 0)); + setColorStopAt(0.2, QColor(180, 10, 0)); + setColorStopAt(0.4, QColor(245, 50, 0)); + setColorStopAt(0.6, QColor(255, 150, 10)); + setColorStopAt(0.8, QColor(255, 255, 50)); + setColorStopAt(1, QColor(255, 255, 255)); + break; + case gpCold: + setColorInterpolation(ciRGB); + setColorStopAt(0, QColor(0, 0, 50)); + setColorStopAt(0.2, QColor(0, 10, 180)); + setColorStopAt(0.4, QColor(0, 50, 245)); + setColorStopAt(0.6, QColor(10, 150, 255)); + setColorStopAt(0.8, QColor(50, 255, 255)); + setColorStopAt(1, QColor(255, 255, 255)); + break; + case gpNight: + setColorInterpolation(ciHSV); + setColorStopAt(0, QColor(10, 20, 30)); + setColorStopAt(1, QColor(250, 255, 250)); + break; + case gpCandy: + setColorInterpolation(ciHSV); + setColorStopAt(0, QColor(0, 0, 255)); + setColorStopAt(1, QColor(255, 250, 250)); + break; + case gpGeography: + setColorInterpolation(ciRGB); + setColorStopAt(0, QColor(70, 170, 210)); + setColorStopAt(0.20, QColor(90, 160, 180)); + setColorStopAt(0.25, QColor(45, 130, 175)); + setColorStopAt(0.30, QColor(100, 140, 125)); + setColorStopAt(0.5, QColor(100, 140, 100)); + setColorStopAt(0.6, QColor(130, 145, 120)); + setColorStopAt(0.7, QColor(140, 130, 120)); + setColorStopAt(0.9, QColor(180, 190, 190)); + setColorStopAt(1, QColor(210, 210, 230)); + break; + case gpIon: + setColorInterpolation(ciHSV); + setColorStopAt(0, QColor(50, 10, 10)); + setColorStopAt(0.45, QColor(0, 0, 255)); + setColorStopAt(0.8, QColor(0, 255, 255)); + setColorStopAt(1, QColor(0, 255, 0)); + break; + case gpThermal: + setColorInterpolation(ciRGB); + setColorStopAt(0, QColor(0, 0, 50)); + setColorStopAt(0.15, QColor(20, 0, 120)); + setColorStopAt(0.33, QColor(200, 30, 140)); + setColorStopAt(0.6, QColor(255, 100, 0)); + setColorStopAt(0.85, QColor(255, 255, 40)); + setColorStopAt(1, QColor(255, 255, 255)); + break; + case gpPolar: + setColorInterpolation(ciRGB); + setColorStopAt(0, QColor(50, 255, 255)); + setColorStopAt(0.18, QColor(10, 70, 255)); + setColorStopAt(0.28, QColor(10, 10, 190)); + setColorStopAt(0.5, QColor(0, 0, 0)); + setColorStopAt(0.72, QColor(190, 10, 10)); + setColorStopAt(0.82, QColor(255, 70, 10)); + setColorStopAt(1, QColor(255, 255, 50)); + break; + case gpSpectrum: + setColorInterpolation(ciHSV); + setColorStopAt(0, QColor(50, 0, 50)); + setColorStopAt(0.15, QColor(0, 0, 255)); + setColorStopAt(0.35, QColor(0, 255, 255)); + setColorStopAt(0.6, QColor(255, 255, 0)); + setColorStopAt(0.75, QColor(255, 30, 0)); + setColorStopAt(1, QColor(50, 0, 0)); + break; + case gpJet: + setColorInterpolation(ciRGB); + setColorStopAt(0, QColor(0, 0, 100)); + setColorStopAt(0.15, QColor(0, 50, 255)); + setColorStopAt(0.35, QColor(0, 255, 255)); + setColorStopAt(0.65, QColor(255, 255, 0)); + setColorStopAt(0.85, QColor(255, 30, 0)); + setColorStopAt(1, QColor(100, 0, 0)); + break; + case gpHues: + setColorInterpolation(ciHSV); + setColorStopAt(0, QColor(255, 0, 0)); + setColorStopAt(1.0/3.0, QColor(0, 0, 255)); + setColorStopAt(2.0/3.0, QColor(0, 255, 0)); + setColorStopAt(1, QColor(255, 0, 0)); + break; + } +} + +/*! + Clears all color stops. + + \see setColorStops, setColorStopAt +*/ +void QCPColorGradient::clearColorStops() +{ + mColorStops.clear(); + mColorBufferInvalidated = true; +} + +/*! + Returns an inverted gradient. The inverted gradient has all properties as this \ref + QCPColorGradient, but the order of the color stops is inverted. + + \see setColorStops, setColorStopAt +*/ +QCPColorGradient QCPColorGradient::inverted() const +{ + QCPColorGradient result(*this); + result.clearColorStops(); + for (QMap::const_iterator it=mColorStops.constBegin(); it!=mColorStops.constEnd(); ++it) + result.setColorStopAt(1.0-it.key(), it.value()); + return result; +} + +/*! \internal + + Updates the internal color buffer which will be used by \ref colorize and \ref color, to quickly + convert positions to colors. This is where the interpolation between color stops is calculated. +*/ +void QCPColorGradient::updateColorBuffer() +{ + if (mColorBuffer.size() != mLevelCount) + mColorBuffer.resize(mLevelCount); + if (mColorStops.size() > 1) + { + double indexToPosFactor = 1.0/(double)(mLevelCount-1); + for (int i=0; i::const_iterator it = mColorStops.lowerBound(position); + if (it == mColorStops.constEnd()) // position is on or after last stop, use color of last stop + { + mColorBuffer[i] = (it-1).value().rgb(); + } else if (it == mColorStops.constBegin()) // position is on or before first stop, use color of first stop + { + mColorBuffer[i] = it.value().rgb(); + } else // position is in between stops (or on an intermediate stop), interpolate color + { + QMap::const_iterator high = it; + QMap::const_iterator low = it-1; + double t = (position-low.key())/(high.key()-low.key()); // interpolation factor 0..1 + switch (mColorInterpolation) + { + case ciRGB: + { + mColorBuffer[i] = qRgb((1-t)*low.value().red() + t*high.value().red(), + (1-t)*low.value().green() + t*high.value().green(), + (1-t)*low.value().blue() + t*high.value().blue()); + break; + } + case ciHSV: + { + QColor lowHsv = low.value().toHsv(); + QColor highHsv = high.value().toHsv(); + double hue = 0; + double hueDiff = highHsv.hueF()-lowHsv.hueF(); + if (hueDiff > 0.5) + hue = lowHsv.hueF() - t*(1.0-hueDiff); + else if (hueDiff < -0.5) + hue = lowHsv.hueF() + t*(1.0+hueDiff); + else + hue = lowHsv.hueF() + t*hueDiff; + if (hue < 0) hue += 1.0; + else if (hue >= 1.0) hue -= 1.0; + mColorBuffer[i] = QColor::fromHsvF(hue, (1-t)*lowHsv.saturationF() + t*highHsv.saturationF(), (1-t)*lowHsv.valueF() + t*highHsv.valueF()).rgb(); + break; + } + } + } + } + } else if (mColorStops.size() == 1) + { + mColorBuffer.fill(mColorStops.constBegin().value().rgb()); + } else // mColorStops is empty, fill color buffer with black + { + mColorBuffer.fill(qRgb(0, 0, 0)); + } + mColorBufferInvalidated = false; +} + + +//////////////////////////////////////////////////////////////////////////////////////////////////// +//////////////////// QCPAxisRect +//////////////////////////////////////////////////////////////////////////////////////////////////// + +/*! \class QCPAxisRect + \brief Holds multiple axes and arranges them in a rectangular shape. + + This class represents an axis rect, a rectangular area that is bounded on all sides with an + arbitrary number of axes. + + Initially QCustomPlot has one axis rect, accessible via QCustomPlot::axisRect(). However, the + layout system allows to have multiple axis rects, e.g. arranged in a grid layout + (QCustomPlot::plotLayout). + + By default, QCPAxisRect comes with four axes, at bottom, top, left and right. They can be + accessed via \ref axis by providing the respective axis type (\ref QCPAxis::AxisType) and index. + If you need all axes in the axis rect, use \ref axes. The top and right axes are set to be + invisible initially (QCPAxis::setVisible). To add more axes to a side, use \ref addAxis or \ref + addAxes. To remove an axis, use \ref removeAxis. + + The axis rect layerable itself only draws a background pixmap or color, if specified (\ref + setBackground). It is placed on the "background" layer initially (see \ref QCPLayer for an + explanation of the QCustomPlot layer system). The axes that are held by the axis rect can be + placed on other layers, independently of the axis rect. + + Every axis rect has a child layout of type \ref QCPLayoutInset. It is accessible via \ref + insetLayout and can be used to have other layout elements (or even other layouts with multiple + elements) hovering inside the axis rect. + + If an axis rect is clicked and dragged, it processes this by moving certain axis ranges. The + behaviour can be controlled with \ref setRangeDrag and \ref setRangeDragAxes. If the mouse wheel + is scrolled while the cursor is on the axis rect, certain axes are scaled. This is controllable + via \ref setRangeZoom, \ref setRangeZoomAxes and \ref setRangeZoomFactor. These interactions are + only enabled if \ref QCustomPlot::setInteractions contains \ref QCP::iRangeDrag and \ref + QCP::iRangeZoom. + + \image html AxisRectSpacingOverview.png +
Overview of the spacings and paddings that define the geometry of an axis. The dashed + line on the far left indicates the viewport/widget border.
+*/ + +/* start documentation of inline functions */ + +/*! \fn QCPLayoutInset *QCPAxisRect::insetLayout() const + + Returns the inset layout of this axis rect. It can be used to place other layout elements (or + even layouts with multiple other elements) inside/on top of an axis rect. + + \see QCPLayoutInset +*/ + +/*! \fn int QCPAxisRect::left() const + + Returns the pixel position of the left border of this axis rect. Margins are not taken into + account here, so the returned value is with respect to the inner \ref rect. +*/ + +/*! \fn int QCPAxisRect::right() const + + Returns the pixel position of the right border of this axis rect. Margins are not taken into + account here, so the returned value is with respect to the inner \ref rect. +*/ + +/*! \fn int QCPAxisRect::top() const + + Returns the pixel position of the top border of this axis rect. Margins are not taken into + account here, so the returned value is with respect to the inner \ref rect. +*/ + +/*! \fn int QCPAxisRect::bottom() const + + Returns the pixel position of the bottom border of this axis rect. Margins are not taken into + account here, so the returned value is with respect to the inner \ref rect. +*/ + +/*! \fn int QCPAxisRect::width() const + + Returns the pixel width of this axis rect. Margins are not taken into account here, so the + returned value is with respect to the inner \ref rect. +*/ + +/*! \fn int QCPAxisRect::height() const + + Returns the pixel height of this axis rect. Margins are not taken into account here, so the + returned value is with respect to the inner \ref rect. +*/ + +/*! \fn QSize QCPAxisRect::size() const + + Returns the pixel size of this axis rect. Margins are not taken into account here, so the + returned value is with respect to the inner \ref rect. +*/ + +/*! \fn QPoint QCPAxisRect::topLeft() const + + Returns the top left corner of this axis rect in pixels. Margins are not taken into account here, + so the returned value is with respect to the inner \ref rect. +*/ + +/*! \fn QPoint QCPAxisRect::topRight() const + + Returns the top right corner of this axis rect in pixels. Margins are not taken into account + here, so the returned value is with respect to the inner \ref rect. +*/ + +/*! \fn QPoint QCPAxisRect::bottomLeft() const + + Returns the bottom left corner of this axis rect in pixels. Margins are not taken into account + here, so the returned value is with respect to the inner \ref rect. +*/ + +/*! \fn QPoint QCPAxisRect::bottomRight() const + + Returns the bottom right corner of this axis rect in pixels. Margins are not taken into account + here, so the returned value is with respect to the inner \ref rect. +*/ + +/*! \fn QPoint QCPAxisRect::center() const + + Returns the center of this axis rect in pixels. Margins are not taken into account here, so the + returned value is with respect to the inner \ref rect. +*/ + +/* end documentation of inline functions */ + +/*! + Creates a QCPAxisRect instance and sets default values. An axis is added for each of the four + sides, the top and right axes are set invisible initially. +*/ +QCPAxisRect::QCPAxisRect(QCustomPlot *parentPlot, bool setupDefaultAxes) : + QCPLayoutElement(parentPlot), + mBackgroundBrush(Qt::NoBrush), + mBackgroundScaled(true), + mBackgroundScaledMode(Qt::KeepAspectRatioByExpanding), + mInsetLayout(new QCPLayoutInset), + mRangeDrag(Qt::Horizontal|Qt::Vertical), + mRangeZoom(Qt::Horizontal|Qt::Vertical), + mRangeZoomFactorHorz(0.85), + mRangeZoomFactorVert(0.85), + mDragging(false) +{ + mInsetLayout->initializeParentPlot(mParentPlot); + mInsetLayout->setParentLayerable(this); + mInsetLayout->setParent(this); + + setMinimumSize(50, 50); + setMinimumMargins(QMargins(15, 15, 15, 15)); + mAxes.insert(QCPAxis::atLeft, QList()); + mAxes.insert(QCPAxis::atRight, QList()); + mAxes.insert(QCPAxis::atTop, QList()); + mAxes.insert(QCPAxis::atBottom, QList()); + + if (setupDefaultAxes) + { + QCPAxis *xAxis = addAxis(QCPAxis::atBottom); + QCPAxis *yAxis = addAxis(QCPAxis::atLeft); + QCPAxis *xAxis2 = addAxis(QCPAxis::atTop); + QCPAxis *yAxis2 = addAxis(QCPAxis::atRight); + setRangeDragAxes(xAxis, yAxis); + setRangeZoomAxes(xAxis, yAxis); + xAxis2->setVisible(false); + yAxis2->setVisible(false); + xAxis->grid()->setVisible(true); + yAxis->grid()->setVisible(true); + xAxis2->grid()->setVisible(false); + yAxis2->grid()->setVisible(false); + xAxis2->grid()->setZeroLinePen(Qt::NoPen); + yAxis2->grid()->setZeroLinePen(Qt::NoPen); + xAxis2->grid()->setVisible(false); + yAxis2->grid()->setVisible(false); + } +} + +QCPAxisRect::~QCPAxisRect() +{ + delete mInsetLayout; + mInsetLayout = 0; + + QList axesList = axes(); + for (int i=0; i ax(mAxes.value(type)); + if (index >= 0 && index < ax.size()) + { + return ax.at(index); + } else + { + qDebug() << Q_FUNC_INFO << "Axis index out of bounds:" << index; + return 0; + } +} + +/*! + Returns all axes on the axis rect sides specified with \a types. + + \a types may be a single \ref QCPAxis::AxisType or an or-combination, to get the axes of + multiple sides. + + \see axis +*/ +QList QCPAxisRect::axes(QCPAxis::AxisTypes types) const +{ + QList result; + if (types.testFlag(QCPAxis::atLeft)) + result << mAxes.value(QCPAxis::atLeft); + if (types.testFlag(QCPAxis::atRight)) + result << mAxes.value(QCPAxis::atRight); + if (types.testFlag(QCPAxis::atTop)) + result << mAxes.value(QCPAxis::atTop); + if (types.testFlag(QCPAxis::atBottom)) + result << mAxes.value(QCPAxis::atBottom); + return result; +} + +/*! \overload + + Returns all axes of this axis rect. +*/ +QList QCPAxisRect::axes() const +{ + QList result; + QHashIterator > it(mAxes); + while (it.hasNext()) + { + it.next(); + result << it.value(); + } + return result; +} + +/*! + Adds a new axis to the axis rect side specified with \a type, and returns it. If \a axis is 0, a + new QCPAxis instance is created internally. + + You may inject QCPAxis instances (or sublasses of QCPAxis) by setting \a axis to an axis that was + previously created outside QCustomPlot. It is important to note that QCustomPlot takes ownership + of the axis, so you may not delete it afterwards. Further, the \a axis must have been created + with this axis rect as parent and with the same axis type as specified in \a type. If this is not + the case, a debug output is generated, the axis is not added, and the method returns 0. + + This method can not be used to move \a axis between axis rects. The same \a axis instance must + not be added multiple times to the same or different axis rects. + + If an axis rect side already contains one or more axes, the lower and upper endings of the new + axis (\ref QCPAxis::setLowerEnding, \ref QCPAxis::setUpperEnding) are set to \ref + QCPLineEnding::esHalfBar. + + \see addAxes, setupFullAxesBox +*/ +QCPAxis *QCPAxisRect::addAxis(QCPAxis::AxisType type, QCPAxis *axis) +{ + QCPAxis *newAxis = axis; + if (!newAxis) + { + newAxis = new QCPAxis(this, type); + } else // user provided existing axis instance, do some sanity checks + { + if (newAxis->axisType() != type) + { + qDebug() << Q_FUNC_INFO << "passed axis has different axis type than specified in type parameter"; + return 0; + } + if (newAxis->axisRect() != this) + { + qDebug() << Q_FUNC_INFO << "passed axis doesn't have this axis rect as parent axis rect"; + return 0; + } + if (axes().contains(newAxis)) + { + qDebug() << Q_FUNC_INFO << "passed axis is already owned by this axis rect"; + return 0; + } + } + if (mAxes[type].size() > 0) // multiple axes on one side, add half-bar axis ending to additional axes with offset + { + bool invert = (type == QCPAxis::atRight) || (type == QCPAxis::atBottom); + newAxis->setLowerEnding(QCPLineEnding(QCPLineEnding::esHalfBar, 6, 10, !invert)); + newAxis->setUpperEnding(QCPLineEnding(QCPLineEnding::esHalfBar, 6, 10, invert)); + } + mAxes[type].append(newAxis); + return newAxis; +} + +/*! + Adds a new axis with \ref addAxis to each axis rect side specified in \a types. This may be an + or-combination of QCPAxis::AxisType, so axes can be added to multiple sides at once. + + Returns a list of the added axes. + + \see addAxis, setupFullAxesBox +*/ +QList QCPAxisRect::addAxes(QCPAxis::AxisTypes types) +{ + QList result; + if (types.testFlag(QCPAxis::atLeft)) + result << addAxis(QCPAxis::atLeft); + if (types.testFlag(QCPAxis::atRight)) + result << addAxis(QCPAxis::atRight); + if (types.testFlag(QCPAxis::atTop)) + result << addAxis(QCPAxis::atTop); + if (types.testFlag(QCPAxis::atBottom)) + result << addAxis(QCPAxis::atBottom); + return result; +} + +/*! + Removes the specified \a axis from the axis rect and deletes it. + + Returns true on success, i.e. if \a axis was a valid axis in this axis rect. + + \see addAxis +*/ +bool QCPAxisRect::removeAxis(QCPAxis *axis) +{ + // don't access axis->axisType() to provide safety when axis is an invalid pointer, rather go through all axis containers: + QHashIterator > it(mAxes); + while (it.hasNext()) + { + it.next(); + if (it.value().contains(axis)) + { + mAxes[it.key()].removeOne(axis); + if (qobject_cast(parentPlot())) // make sure this isn't called from QObject dtor when QCustomPlot is already destructed (happens when the axis rect is not in any layout and thus QObject-child of QCustomPlot) + parentPlot()->axisRemoved(axis); + delete axis; + return true; + } + } + qDebug() << Q_FUNC_INFO << "Axis isn't in axis rect:" << reinterpret_cast(axis); + return false; +} + +/*! + Convenience function to create an axis on each side that doesn't have any axes yet and set their + visibility to true. Further, the top/right axes are assigned the following properties of the + bottom/left axes: + + \li range (\ref QCPAxis::setRange) + \li range reversed (\ref QCPAxis::setRangeReversed) + \li scale type (\ref QCPAxis::setScaleType) + \li scale log base (\ref QCPAxis::setScaleLogBase) + \li ticks (\ref QCPAxis::setTicks) + \li auto (major) tick count (\ref QCPAxis::setAutoTickCount) + \li sub tick count (\ref QCPAxis::setSubTickCount) + \li auto sub ticks (\ref QCPAxis::setAutoSubTicks) + \li tick step (\ref QCPAxis::setTickStep) + \li auto tick step (\ref QCPAxis::setAutoTickStep) + \li number format (\ref QCPAxis::setNumberFormat) + \li number precision (\ref QCPAxis::setNumberPrecision) + \li tick label type (\ref QCPAxis::setTickLabelType) + \li date time format (\ref QCPAxis::setDateTimeFormat) + \li date time spec (\ref QCPAxis::setDateTimeSpec) + + Tick labels (\ref QCPAxis::setTickLabels) of the right and top axes are set to false. + + If \a connectRanges is true, the \ref QCPAxis::rangeChanged "rangeChanged" signals of the bottom + and left axes are connected to the \ref QCPAxis::setRange slots of the top and right axes. +*/ +void QCPAxisRect::setupFullAxesBox(bool connectRanges) +{ + QCPAxis *xAxis, *yAxis, *xAxis2, *yAxis2; + if (axisCount(QCPAxis::atBottom) == 0) + xAxis = addAxis(QCPAxis::atBottom); + else + xAxis = axis(QCPAxis::atBottom); + + if (axisCount(QCPAxis::atLeft) == 0) + yAxis = addAxis(QCPAxis::atLeft); + else + yAxis = axis(QCPAxis::atLeft); + + if (axisCount(QCPAxis::atTop) == 0) + xAxis2 = addAxis(QCPAxis::atTop); + else + xAxis2 = axis(QCPAxis::atTop); + + if (axisCount(QCPAxis::atRight) == 0) + yAxis2 = addAxis(QCPAxis::atRight); + else + yAxis2 = axis(QCPAxis::atRight); + + xAxis->setVisible(true); + yAxis->setVisible(true); + xAxis2->setVisible(true); + yAxis2->setVisible(true); + xAxis2->setTickLabels(false); + yAxis2->setTickLabels(false); + + xAxis2->setRange(xAxis->range()); + xAxis2->setRangeReversed(xAxis->rangeReversed()); + xAxis2->setScaleType(xAxis->scaleType()); + xAxis2->setScaleLogBase(xAxis->scaleLogBase()); + xAxis2->setTicks(xAxis->ticks()); + xAxis2->setAutoTickCount(xAxis->autoTickCount()); + xAxis2->setSubTickCount(xAxis->subTickCount()); + xAxis2->setAutoSubTicks(xAxis->autoSubTicks()); + xAxis2->setTickStep(xAxis->tickStep()); + xAxis2->setAutoTickStep(xAxis->autoTickStep()); + xAxis2->setNumberFormat(xAxis->numberFormat()); + xAxis2->setNumberPrecision(xAxis->numberPrecision()); + xAxis2->setTickLabelType(xAxis->tickLabelType()); + xAxis2->setDateTimeFormat(xAxis->dateTimeFormat()); + xAxis2->setDateTimeSpec(xAxis->dateTimeSpec()); + + yAxis2->setRange(yAxis->range()); + yAxis2->setRangeReversed(yAxis->rangeReversed()); + yAxis2->setScaleType(yAxis->scaleType()); + yAxis2->setScaleLogBase(yAxis->scaleLogBase()); + yAxis2->setTicks(yAxis->ticks()); + yAxis2->setAutoTickCount(yAxis->autoTickCount()); + yAxis2->setSubTickCount(yAxis->subTickCount()); + yAxis2->setAutoSubTicks(yAxis->autoSubTicks()); + yAxis2->setTickStep(yAxis->tickStep()); + yAxis2->setAutoTickStep(yAxis->autoTickStep()); + yAxis2->setNumberFormat(yAxis->numberFormat()); + yAxis2->setNumberPrecision(yAxis->numberPrecision()); + yAxis2->setTickLabelType(yAxis->tickLabelType()); + yAxis2->setDateTimeFormat(yAxis->dateTimeFormat()); + yAxis2->setDateTimeSpec(yAxis->dateTimeSpec()); + + if (connectRanges) + { + connect(xAxis, SIGNAL(rangeChanged(QCPRange)), xAxis2, SLOT(setRange(QCPRange))); + connect(yAxis, SIGNAL(rangeChanged(QCPRange)), yAxis2, SLOT(setRange(QCPRange))); + } +} + +/*! + Returns a list of all the plottables that are associated with this axis rect. + + A plottable is considered associated with an axis rect if its key or value axis (or both) is in + this axis rect. + + \see graphs, items +*/ +QList QCPAxisRect::plottables() const +{ + // Note: don't append all QCPAxis::plottables() into a list, because we might get duplicate entries + QList result; + for (int i=0; imPlottables.size(); ++i) + { + if (mParentPlot->mPlottables.at(i)->keyAxis()->axisRect() == this ||mParentPlot->mPlottables.at(i)->valueAxis()->axisRect() == this) + result.append(mParentPlot->mPlottables.at(i)); + } + return result; +} + +/*! + Returns a list of all the graphs that are associated with this axis rect. + + A graph is considered associated with an axis rect if its key or value axis (or both) is in + this axis rect. + + \see plottables, items +*/ +QList QCPAxisRect::graphs() const +{ + // Note: don't append all QCPAxis::graphs() into a list, because we might get duplicate entries + QList result; + for (int i=0; imGraphs.size(); ++i) + { + if (mParentPlot->mGraphs.at(i)->keyAxis()->axisRect() == this || mParentPlot->mGraphs.at(i)->valueAxis()->axisRect() == this) + result.append(mParentPlot->mGraphs.at(i)); + } + return result; +} + +/*! + Returns a list of all the items that are associated with this axis rect. + + An item is considered associated with an axis rect if any of its positions has key or value axis + set to an axis that is in this axis rect, or if any of its positions has \ref + QCPItemPosition::setAxisRect set to the axis rect, or if the clip axis rect (\ref + QCPAbstractItem::setClipAxisRect) is set to this axis rect. + + \see plottables, graphs +*/ +QList QCPAxisRect::items() const +{ + // Note: don't just append all QCPAxis::items() into a list, because we might get duplicate entries + // and miss those items that have this axis rect as clipAxisRect. + QList result; + for (int itemId=0; itemIdmItems.size(); ++itemId) + { + if (mParentPlot->mItems.at(itemId)->clipAxisRect() == this) + { + result.append(mParentPlot->mItems.at(itemId)); + continue; + } + QList positions = mParentPlot->mItems.at(itemId)->positions(); + for (int posId=0; posIdaxisRect() == this || + positions.at(posId)->keyAxis()->axisRect() == this || + positions.at(posId)->valueAxis()->axisRect() == this) + { + result.append(mParentPlot->mItems.at(itemId)); + break; + } + } + } + return result; +} + +/*! + This method is called automatically upon replot and doesn't need to be called by users of + QCPAxisRect. + + Calls the base class implementation to update the margins (see \ref QCPLayoutElement::update), + and finally passes the \ref rect to the inset layout (\ref insetLayout) and calls its + QCPInsetLayout::update function. +*/ +void QCPAxisRect::update(UpdatePhase phase) +{ + QCPLayoutElement::update(phase); + + switch (phase) + { + case upPreparation: + { + QList allAxes = axes(); + for (int i=0; isetupTickVectors(); + break; + } + case upLayout: + { + mInsetLayout->setOuterRect(rect()); + break; + } + default: break; + } + + // pass update call on to inset layout (doesn't happen automatically, because QCPAxisRect doesn't derive from QCPLayout): + mInsetLayout->update(phase); +} + +/* inherits documentation from base class */ +QList QCPAxisRect::elements(bool recursive) const +{ + QList result; + if (mInsetLayout) + { + result << mInsetLayout; + if (recursive) + result << mInsetLayout->elements(recursive); + } + return result; +} + +/* inherits documentation from base class */ +void QCPAxisRect::applyDefaultAntialiasingHint(QCPPainter *painter) const +{ + painter->setAntialiasing(false); +} + +/* inherits documentation from base class */ +void QCPAxisRect::draw(QCPPainter *painter) +{ + drawBackground(painter); +} + +/*! + Sets \a pm as the axis background pixmap. The axis background pixmap will be drawn inside the + axis rect. Since axis rects place themselves on the "background" layer by default, the axis rect + backgrounds are usually drawn below everything else. + + For cases where the provided pixmap doesn't have the same size as the axis rect, scaling can be + enabled with \ref setBackgroundScaled and the scaling mode (i.e. whether and how the aspect ratio + is preserved) can be set with \ref setBackgroundScaledMode. To set all these options in one call, + consider using the overloaded version of this function. + + Below the pixmap, the axis rect may be optionally filled with a brush, if specified with \ref + setBackground(const QBrush &brush). + + \see setBackgroundScaled, setBackgroundScaledMode, setBackground(const QBrush &brush) +*/ +void QCPAxisRect::setBackground(const QPixmap &pm) +{ + mBackgroundPixmap = pm; + mScaledBackgroundPixmap = QPixmap(); +} + +/*! \overload + + Sets \a brush as the background brush. The axis rect background will be filled with this brush. + Since axis rects place themselves on the "background" layer by default, the axis rect backgrounds + are usually drawn below everything else. + + The brush will be drawn before (under) any background pixmap, which may be specified with \ref + setBackground(const QPixmap &pm). + + To disable drawing of a background brush, set \a brush to Qt::NoBrush. + + \see setBackground(const QPixmap &pm) +*/ +void QCPAxisRect::setBackground(const QBrush &brush) +{ + mBackgroundBrush = brush; +} + +/*! \overload + + Allows setting the background pixmap of the axis rect, whether it shall be scaled and how it + shall be scaled in one call. + + \see setBackground(const QPixmap &pm), setBackgroundScaled, setBackgroundScaledMode +*/ +void QCPAxisRect::setBackground(const QPixmap &pm, bool scaled, Qt::AspectRatioMode mode) +{ + mBackgroundPixmap = pm; + mScaledBackgroundPixmap = QPixmap(); + mBackgroundScaled = scaled; + mBackgroundScaledMode = mode; +} + +/*! + Sets whether the axis background pixmap shall be scaled to fit the axis rect or not. If \a scaled + is set to true, you may control whether and how the aspect ratio of the original pixmap is + preserved with \ref setBackgroundScaledMode. + + Note that the scaled version of the original pixmap is buffered, so there is no performance + penalty on replots. (Except when the axis rect dimensions are changed continuously.) + + \see setBackground, setBackgroundScaledMode +*/ +void QCPAxisRect::setBackgroundScaled(bool scaled) +{ + mBackgroundScaled = scaled; +} + +/*! + If scaling of the axis background pixmap is enabled (\ref setBackgroundScaled), use this function to + define whether and how the aspect ratio of the original pixmap passed to \ref setBackground is preserved. + \see setBackground, setBackgroundScaled +*/ +void QCPAxisRect::setBackgroundScaledMode(Qt::AspectRatioMode mode) +{ + mBackgroundScaledMode = mode; +} + +/*! + Returns the range drag axis of the \a orientation provided. + + \see setRangeDragAxes +*/ +QCPAxis *QCPAxisRect::rangeDragAxis(Qt::Orientation orientation) +{ + return (orientation == Qt::Horizontal ? mRangeDragHorzAxis.data() : mRangeDragVertAxis.data()); +} + +/*! + Returns the range zoom axis of the \a orientation provided. + + \see setRangeZoomAxes +*/ +QCPAxis *QCPAxisRect::rangeZoomAxis(Qt::Orientation orientation) +{ + return (orientation == Qt::Horizontal ? mRangeZoomHorzAxis.data() : mRangeZoomVertAxis.data()); +} + +/*! + Returns the range zoom factor of the \a orientation provided. + + \see setRangeZoomFactor +*/ +double QCPAxisRect::rangeZoomFactor(Qt::Orientation orientation) +{ + return (orientation == Qt::Horizontal ? mRangeZoomFactorHorz : mRangeZoomFactorVert); +} + +/*! + Sets which axis orientation may be range dragged by the user with mouse interaction. + What orientation corresponds to which specific axis can be set with + \ref setRangeDragAxes(QCPAxis *horizontal, QCPAxis *vertical). By + default, the horizontal axis is the bottom axis (xAxis) and the vertical axis + is the left axis (yAxis). + + To disable range dragging entirely, pass 0 as \a orientations or remove \ref QCP::iRangeDrag from \ref + QCustomPlot::setInteractions. To enable range dragging for both directions, pass Qt::Horizontal | + Qt::Vertical as \a orientations. + + In addition to setting \a orientations to a non-zero value, make sure \ref QCustomPlot::setInteractions + contains \ref QCP::iRangeDrag to enable the range dragging interaction. + + \see setRangeZoom, setRangeDragAxes, QCustomPlot::setNoAntialiasingOnDrag +*/ +void QCPAxisRect::setRangeDrag(Qt::Orientations orientations) +{ + mRangeDrag = orientations; +} + +/*! + Sets which axis orientation may be zoomed by the user with the mouse wheel. What orientation + corresponds to which specific axis can be set with \ref setRangeZoomAxes(QCPAxis *horizontal, + QCPAxis *vertical). By default, the horizontal axis is the bottom axis (xAxis) and the vertical + axis is the left axis (yAxis). + + To disable range zooming entirely, pass 0 as \a orientations or remove \ref QCP::iRangeZoom from \ref + QCustomPlot::setInteractions. To enable range zooming for both directions, pass Qt::Horizontal | + Qt::Vertical as \a orientations. + + In addition to setting \a orientations to a non-zero value, make sure \ref QCustomPlot::setInteractions + contains \ref QCP::iRangeZoom to enable the range zooming interaction. + + \see setRangeZoomFactor, setRangeZoomAxes, setRangeDrag +*/ +void QCPAxisRect::setRangeZoom(Qt::Orientations orientations) +{ + mRangeZoom = orientations; +} + +/*! + Sets the axes whose range will be dragged when \ref setRangeDrag enables mouse range dragging + on the QCustomPlot widget. + + \see setRangeZoomAxes +*/ +void QCPAxisRect::setRangeDragAxes(QCPAxis *horizontal, QCPAxis *vertical) +{ + mRangeDragHorzAxis = horizontal; + mRangeDragVertAxis = vertical; +} + +/*! + Sets the axes whose range will be zoomed when \ref setRangeZoom enables mouse wheel zooming on the + QCustomPlot widget. The two axes can be zoomed with different strengths, when different factors + are passed to \ref setRangeZoomFactor(double horizontalFactor, double verticalFactor). + + \see setRangeDragAxes +*/ +void QCPAxisRect::setRangeZoomAxes(QCPAxis *horizontal, QCPAxis *vertical) +{ + mRangeZoomHorzAxis = horizontal; + mRangeZoomVertAxis = vertical; +} + +/*! + Sets how strong one rotation step of the mouse wheel zooms, when range zoom was activated with + \ref setRangeZoom. The two parameters \a horizontalFactor and \a verticalFactor provide a way to + let the horizontal axis zoom at different rates than the vertical axis. Which axis is horizontal + and which is vertical, can be set with \ref setRangeZoomAxes. + + When the zoom factor is greater than one, scrolling the mouse wheel backwards (towards the user) + will zoom in (make the currently visible range smaller). For zoom factors smaller than one, the + same scrolling direction will zoom out. +*/ +void QCPAxisRect::setRangeZoomFactor(double horizontalFactor, double verticalFactor) +{ + mRangeZoomFactorHorz = horizontalFactor; + mRangeZoomFactorVert = verticalFactor; +} + +/*! \overload + + Sets both the horizontal and vertical zoom \a factor. +*/ +void QCPAxisRect::setRangeZoomFactor(double factor) +{ + mRangeZoomFactorHorz = factor; + mRangeZoomFactorVert = factor; +} + +/*! \internal + + Draws the background of this axis rect. It may consist of a background fill (a QBrush) and a + pixmap. + + If a brush was given via \ref setBackground(const QBrush &brush), this function first draws an + according filling inside the axis rect with the provided \a painter. + + Then, if a pixmap was provided via \ref setBackground, this function buffers the scaled version + depending on \ref setBackgroundScaled and \ref setBackgroundScaledMode and then draws it inside + the axis rect with the provided \a painter. The scaled version is buffered in + mScaledBackgroundPixmap to prevent expensive rescaling at every redraw. It is only updated, when + the axis rect has changed in a way that requires a rescale of the background pixmap (this is + dependant on the \ref setBackgroundScaledMode), or when a differend axis backgroud pixmap was + set. + + \see setBackground, setBackgroundScaled, setBackgroundScaledMode +*/ +void QCPAxisRect::drawBackground(QCPPainter *painter) +{ + // draw background fill: + if (mBackgroundBrush != Qt::NoBrush) + painter->fillRect(mRect, mBackgroundBrush); + + // draw background pixmap (on top of fill, if brush specified): + if (!mBackgroundPixmap.isNull()) + { + if (mBackgroundScaled) + { + // check whether mScaledBackground needs to be updated: + QSize scaledSize(mBackgroundPixmap.size()); + scaledSize.scale(mRect.size(), mBackgroundScaledMode); + if (mScaledBackgroundPixmap.size() != scaledSize) + mScaledBackgroundPixmap = mBackgroundPixmap.scaled(mRect.size(), mBackgroundScaledMode, Qt::SmoothTransformation); + painter->drawPixmap(mRect.topLeft()+QPoint(0, -1), mScaledBackgroundPixmap, QRect(0, 0, mRect.width(), mRect.height()) & mScaledBackgroundPixmap.rect()); + } else + { + painter->drawPixmap(mRect.topLeft()+QPoint(0, -1), mBackgroundPixmap, QRect(0, 0, mRect.width(), mRect.height())); + } + } +} + +/*! \internal + + This function makes sure multiple axes on the side specified with \a type don't collide, but are + distributed according to their respective space requirement (QCPAxis::calculateMargin). + + It does this by setting an appropriate offset (\ref QCPAxis::setOffset) on all axes except the + one with index zero. + + This function is called by \ref calculateAutoMargin. +*/ +void QCPAxisRect::updateAxesOffset(QCPAxis::AxisType type) +{ + const QList axesList = mAxes.value(type); + if (axesList.isEmpty()) + return; + + bool isFirstVisible = !axesList.first()->visible(); // if the first axis is visible, the second axis (which is where the loop starts) isn't the first visible axis, so initialize with false + for (int i=1; ioffset() + axesList.at(i-1)->calculateMargin(); + if (axesList.at(i)->visible()) // only add inner tick length to offset if this axis is visible and it's not the first visible one (might happen if true first axis is invisible) + { + if (!isFirstVisible) + offset += axesList.at(i)->tickLengthIn(); + isFirstVisible = false; + } + axesList.at(i)->setOffset(offset); + } +} + +/* inherits documentation from base class */ +int QCPAxisRect::calculateAutoMargin(QCP::MarginSide side) +{ + if (!mAutoMargins.testFlag(side)) + qDebug() << Q_FUNC_INFO << "Called with side that isn't specified as auto margin"; + + updateAxesOffset(QCPAxis::marginSideToAxisType(side)); + + // note: only need to look at the last (outer most) axis to determine the total margin, due to updateAxisOffset call + const QList axesList = mAxes.value(QCPAxis::marginSideToAxisType(side)); + if (axesList.size() > 0) + return axesList.last()->offset() + axesList.last()->calculateMargin(); + else + return 0; +} + +/*! \internal + + Event handler for when a mouse button is pressed on the axis rect. If the left mouse button is + pressed, the range dragging interaction is initialized (the actual range manipulation happens in + the \ref mouseMoveEvent). + + The mDragging flag is set to true and some anchor points are set that are needed to determine the + distance the mouse was dragged in the mouse move/release events later. + + \see mouseMoveEvent, mouseReleaseEvent +*/ +void QCPAxisRect::mousePressEvent(QMouseEvent *event) +{ + mDragStart = event->pos(); // need this even when not LeftButton is pressed, to determine in releaseEvent whether it was a full click (no position change between press and release) + if (event->buttons() & Qt::LeftButton) + { + mDragging = true; + // initialize antialiasing backup in case we start dragging: + if (mParentPlot->noAntialiasingOnDrag()) + { + mAADragBackup = mParentPlot->antialiasedElements(); + mNotAADragBackup = mParentPlot->notAntialiasedElements(); + } + // Mouse range dragging interaction: + if (mParentPlot->interactions().testFlag(QCP::iRangeDrag)) + { + if (mRangeDragHorzAxis) + mDragStartHorzRange = mRangeDragHorzAxis.data()->range(); + if (mRangeDragVertAxis) + mDragStartVertRange = mRangeDragVertAxis.data()->range(); + } + } +} + +/*! \internal + + Event handler for when the mouse is moved on the axis rect. If range dragging was activated in a + preceding \ref mousePressEvent, the range is moved accordingly. + + \see mousePressEvent, mouseReleaseEvent +*/ +void QCPAxisRect::mouseMoveEvent(QMouseEvent *event) +{ + // Mouse range dragging interaction: + if (mDragging && mParentPlot->interactions().testFlag(QCP::iRangeDrag)) + { + if (mRangeDrag.testFlag(Qt::Horizontal)) + { + if (QCPAxis *rangeDragHorzAxis = mRangeDragHorzAxis.data()) + { + if (rangeDragHorzAxis->mScaleType == QCPAxis::stLinear) + { + double diff = rangeDragHorzAxis->pixelToCoord(mDragStart.x()) - rangeDragHorzAxis->pixelToCoord(event->pos().x()); + rangeDragHorzAxis->setRange(mDragStartHorzRange.lower+diff, mDragStartHorzRange.upper+diff); + } else if (rangeDragHorzAxis->mScaleType == QCPAxis::stLogarithmic) + { + double diff = rangeDragHorzAxis->pixelToCoord(mDragStart.x()) / rangeDragHorzAxis->pixelToCoord(event->pos().x()); + rangeDragHorzAxis->setRange(mDragStartHorzRange.lower*diff, mDragStartHorzRange.upper*diff); + } + } + } + if (mRangeDrag.testFlag(Qt::Vertical)) + { + if (QCPAxis *rangeDragVertAxis = mRangeDragVertAxis.data()) + { + if (rangeDragVertAxis->mScaleType == QCPAxis::stLinear) + { + double diff = rangeDragVertAxis->pixelToCoord(mDragStart.y()) - rangeDragVertAxis->pixelToCoord(event->pos().y()); + rangeDragVertAxis->setRange(mDragStartVertRange.lower+diff, mDragStartVertRange.upper+diff); + } else if (rangeDragVertAxis->mScaleType == QCPAxis::stLogarithmic) + { + double diff = rangeDragVertAxis->pixelToCoord(mDragStart.y()) / rangeDragVertAxis->pixelToCoord(event->pos().y()); + rangeDragVertAxis->setRange(mDragStartVertRange.lower*diff, mDragStartVertRange.upper*diff); + } + } + } + if (mRangeDrag != 0) // if either vertical or horizontal drag was enabled, do a replot + { + if (mParentPlot->noAntialiasingOnDrag()) + mParentPlot->setNotAntialiasedElements(QCP::aeAll); + mParentPlot->replot(); + } + } +} + +/* inherits documentation from base class */ +void QCPAxisRect::mouseReleaseEvent(QMouseEvent *event) +{ + Q_UNUSED(event) + mDragging = false; + if (mParentPlot->noAntialiasingOnDrag()) + { + mParentPlot->setAntialiasedElements(mAADragBackup); + mParentPlot->setNotAntialiasedElements(mNotAADragBackup); + } +} + +/*! \internal + + Event handler for mouse wheel events. If rangeZoom is Qt::Horizontal, Qt::Vertical or both, the + ranges of the axes defined as rangeZoomHorzAxis and rangeZoomVertAxis are scaled. The center of + the scaling operation is the current cursor position inside the axis rect. The scaling factor is + dependant on the mouse wheel delta (which direction the wheel was rotated) to provide a natural + zooming feel. The Strength of the zoom can be controlled via \ref setRangeZoomFactor. + + Note, that event->delta() is usually +/-120 for single rotation steps. However, if the mouse + wheel is turned rapidly, many steps may bunch up to one event, so the event->delta() may then be + multiples of 120. This is taken into account here, by calculating \a wheelSteps and using it as + exponent of the range zoom factor. This takes care of the wheel direction automatically, by + inverting the factor, when the wheel step is negative (f^-1 = 1/f). +*/ +void QCPAxisRect::wheelEvent(QWheelEvent *event) +{ + // Mouse range zooming interaction: + if (mParentPlot->interactions().testFlag(QCP::iRangeZoom)) + { + if (mRangeZoom != 0) + { + double factor; + double wheelSteps = event->delta()/120.0; // a single step delta is +/-120 usually + if (mRangeZoom.testFlag(Qt::Horizontal)) + { + factor = qPow(mRangeZoomFactorHorz, wheelSteps); + if (mRangeZoomHorzAxis.data()) + mRangeZoomHorzAxis.data()->scaleRange(factor, mRangeZoomHorzAxis.data()->pixelToCoord(event->pos().x())); + } + if (mRangeZoom.testFlag(Qt::Vertical)) + { + factor = qPow(mRangeZoomFactorVert, wheelSteps); + if (mRangeZoomVertAxis.data()) + mRangeZoomVertAxis.data()->scaleRange(factor, mRangeZoomVertAxis.data()->pixelToCoord(event->pos().y())); + } + mParentPlot->replot(); + } + } +} + + +//////////////////////////////////////////////////////////////////////////////////////////////////// +//////////////////// QCPAbstractLegendItem +//////////////////////////////////////////////////////////////////////////////////////////////////// + +/*! \class QCPAbstractLegendItem + \brief The abstract base class for all entries in a QCPLegend. + + It defines a very basic interface for entries in a QCPLegend. For representing plottables in the + legend, the subclass \ref QCPPlottableLegendItem is more suitable. + + Only derive directly from this class when you need absolute freedom (e.g. a custom legend entry + that's not even associated with a plottable). + + You must implement the following pure virtual functions: + \li \ref draw (from QCPLayerable) + + You inherit the following members you may use: + + + + + + + + +
QCPLegend *\b mParentLegendA pointer to the parent QCPLegend.
QFont \b mFontThe generic font of the item. You should use this font for all or at least the most prominent text of the item.
+*/ + +/* start of documentation of signals */ + +/*! \fn void QCPAbstractLegendItem::selectionChanged(bool selected) + + This signal is emitted when the selection state of this legend item has changed, either by user + interaction or by a direct call to \ref setSelected. +*/ + +/* end of documentation of signals */ + +/*! + Constructs a QCPAbstractLegendItem and associates it with the QCPLegend \a parent. This does not + cause the item to be added to \a parent, so \ref QCPLegend::addItem must be called separately. +*/ +QCPAbstractLegendItem::QCPAbstractLegendItem(QCPLegend *parent) : + QCPLayoutElement(parent->parentPlot()), + mParentLegend(parent), + mFont(parent->font()), + mTextColor(parent->textColor()), + mSelectedFont(parent->selectedFont()), + mSelectedTextColor(parent->selectedTextColor()), + mSelectable(true), + mSelected(false) +{ + setLayer(QLatin1String("legend")); + setMargins(QMargins(8, 2, 8, 2)); +} + +/*! + Sets the default font of this specific legend item to \a font. + + \see setTextColor, QCPLegend::setFont +*/ +void QCPAbstractLegendItem::setFont(const QFont &font) +{ + mFont = font; +} + +/*! + Sets the default text color of this specific legend item to \a color. + + \see setFont, QCPLegend::setTextColor +*/ +void QCPAbstractLegendItem::setTextColor(const QColor &color) +{ + mTextColor = color; +} + +/*! + When this legend item is selected, \a font is used to draw generic text, instead of the normal + font set with \ref setFont. + + \see setFont, QCPLegend::setSelectedFont +*/ +void QCPAbstractLegendItem::setSelectedFont(const QFont &font) +{ + mSelectedFont = font; +} + +/*! + When this legend item is selected, \a color is used to draw generic text, instead of the normal + color set with \ref setTextColor. + + \see setTextColor, QCPLegend::setSelectedTextColor +*/ +void QCPAbstractLegendItem::setSelectedTextColor(const QColor &color) +{ + mSelectedTextColor = color; +} + +/*! + Sets whether this specific legend item is selectable. + + \see setSelectedParts, QCustomPlot::setInteractions +*/ +void QCPAbstractLegendItem::setSelectable(bool selectable) +{ + if (mSelectable != selectable) + { + mSelectable = selectable; + emit selectableChanged(mSelectable); + } +} + +/*! + Sets whether this specific legend item is selected. + + It is possible to set the selection state of this item by calling this function directly, even if + setSelectable is set to false. + + \see setSelectableParts, QCustomPlot::setInteractions +*/ +void QCPAbstractLegendItem::setSelected(bool selected) +{ + if (mSelected != selected) + { + mSelected = selected; + emit selectionChanged(mSelected); + } +} + +/* inherits documentation from base class */ +double QCPAbstractLegendItem::selectTest(const QPointF &pos, bool onlySelectable, QVariant *details) const +{ + Q_UNUSED(details) + if (!mParentPlot) return -1; + if (onlySelectable && (!mSelectable || !mParentLegend->selectableParts().testFlag(QCPLegend::spItems))) + return -1; + + if (mRect.contains(pos.toPoint())) + return mParentPlot->selectionTolerance()*0.99; + else + return -1; +} + +/* inherits documentation from base class */ +void QCPAbstractLegendItem::applyDefaultAntialiasingHint(QCPPainter *painter) const +{ + applyAntialiasingHint(painter, mAntialiased, QCP::aeLegendItems); +} + +/* inherits documentation from base class */ +QRect QCPAbstractLegendItem::clipRect() const +{ + return mOuterRect; +} + +/* inherits documentation from base class */ +void QCPAbstractLegendItem::selectEvent(QMouseEvent *event, bool additive, const QVariant &details, bool *selectionStateChanged) +{ + Q_UNUSED(event) + Q_UNUSED(details) + if (mSelectable && mParentLegend->selectableParts().testFlag(QCPLegend::spItems)) + { + bool selBefore = mSelected; + setSelected(additive ? !mSelected : true); + if (selectionStateChanged) + *selectionStateChanged = mSelected != selBefore; + } +} + +/* inherits documentation from base class */ +void QCPAbstractLegendItem::deselectEvent(bool *selectionStateChanged) +{ + if (mSelectable && mParentLegend->selectableParts().testFlag(QCPLegend::spItems)) + { + bool selBefore = mSelected; + setSelected(false); + if (selectionStateChanged) + *selectionStateChanged = mSelected != selBefore; + } +} + +//////////////////////////////////////////////////////////////////////////////////////////////////// +//////////////////// QCPPlottableLegendItem +//////////////////////////////////////////////////////////////////////////////////////////////////// + +/*! \class QCPPlottableLegendItem + \brief A legend item representing a plottable with an icon and the plottable name. + + This is the standard legend item for plottables. It displays an icon of the plottable next to the + plottable name. The icon is drawn by the respective plottable itself (\ref + QCPAbstractPlottable::drawLegendIcon), and tries to give an intuitive symbol for the plottable. + For example, the QCPGraph draws a centered horizontal line and/or a single scatter point in the + middle. + + Legend items of this type are always associated with one plottable (retrievable via the + plottable() function and settable with the constructor). You may change the font of the plottable + name with \ref setFont. Icon padding and border pen is taken from the parent QCPLegend, see \ref + QCPLegend::setIconBorderPen and \ref QCPLegend::setIconTextPadding. + + The function \ref QCPAbstractPlottable::addToLegend/\ref QCPAbstractPlottable::removeFromLegend + creates/removes legend items of this type in the default implementation. However, these functions + may be reimplemented such that a different kind of legend item (e.g a direct subclass of + QCPAbstractLegendItem) is used for that plottable. + + Since QCPLegend is based on QCPLayoutGrid, a legend item itself is just a subclass of + QCPLayoutElement. While it could be added to a legend (or any other layout) via the normal layout + interface, QCPLegend has specialized functions for handling legend items conveniently, see the + documentation of \ref QCPLegend. +*/ + +/*! + Creates a new legend item associated with \a plottable. + + Once it's created, it can be added to the legend via \ref QCPLegend::addItem. + + A more convenient way of adding/removing a plottable to/from the legend is via the functions \ref + QCPAbstractPlottable::addToLegend and \ref QCPAbstractPlottable::removeFromLegend. +*/ +QCPPlottableLegendItem::QCPPlottableLegendItem(QCPLegend *parent, QCPAbstractPlottable *plottable) : + QCPAbstractLegendItem(parent), + mPlottable(plottable) +{ +} + +/*! \internal + + Returns the pen that shall be used to draw the icon border, taking into account the selection + state of this item. +*/ +QPen QCPPlottableLegendItem::getIconBorderPen() const +{ + return mSelected ? mParentLegend->selectedIconBorderPen() : mParentLegend->iconBorderPen(); +} + +/*! \internal + + Returns the text color that shall be used to draw text, taking into account the selection state + of this item. +*/ +QColor QCPPlottableLegendItem::getTextColor() const +{ + return mSelected ? mSelectedTextColor : mTextColor; +} + +/*! \internal + + Returns the font that shall be used to draw text, taking into account the selection state of this + item. +*/ +QFont QCPPlottableLegendItem::getFont() const +{ + return mSelected ? mSelectedFont : mFont; +} + +/*! \internal + + Draws the item with \a painter. The size and position of the drawn legend item is defined by the + parent layout (typically a \ref QCPLegend) and the \ref minimumSizeHint and \ref maximumSizeHint + of this legend item. +*/ +void QCPPlottableLegendItem::draw(QCPPainter *painter) +{ + if (!mPlottable) return; + painter->setFont(getFont()); + painter->setPen(QPen(getTextColor())); + QSizeF iconSize = mParentLegend->iconSize(); + QRectF textRect = painter->fontMetrics().boundingRect(0, 0, 0, iconSize.height(), Qt::TextDontClip, mPlottable->name()); + QRectF iconRect(mRect.topLeft(), iconSize); + int textHeight = qMax(textRect.height(), iconSize.height()); // if text has smaller height than icon, center text vertically in icon height, else align tops + painter->drawText(mRect.x()+iconSize.width()+mParentLegend->iconTextPadding(), mRect.y(), textRect.width(), textHeight, Qt::TextDontClip, mPlottable->name()); + // draw icon: + painter->save(); + painter->setClipRect(iconRect, Qt::IntersectClip); + mPlottable->drawLegendIcon(painter, iconRect); + painter->restore(); + // draw icon border: + if (getIconBorderPen().style() != Qt::NoPen) + { + painter->setPen(getIconBorderPen()); + painter->setBrush(Qt::NoBrush); + painter->drawRect(iconRect); + } +} + +/*! \internal + + Calculates and returns the size of this item. This includes the icon, the text and the padding in + between. +*/ +QSize QCPPlottableLegendItem::minimumSizeHint() const +{ + if (!mPlottable) return QSize(); + QSize result(0, 0); + QRect textRect; + QFontMetrics fontMetrics(getFont()); + QSize iconSize = mParentLegend->iconSize(); + textRect = fontMetrics.boundingRect(0, 0, 0, iconSize.height(), Qt::TextDontClip, mPlottable->name()); + result.setWidth(iconSize.width() + mParentLegend->iconTextPadding() + textRect.width() + mMargins.left() + mMargins.right()); + result.setHeight(qMax(textRect.height(), iconSize.height()) + mMargins.top() + mMargins.bottom()); + return result; +} + + +//////////////////////////////////////////////////////////////////////////////////////////////////// +//////////////////// QCPLegend +//////////////////////////////////////////////////////////////////////////////////////////////////// + +/*! \class QCPLegend + \brief Manages a legend inside a QCustomPlot. + + A legend is a small box somewhere in the plot which lists plottables with their name and icon. + + Normally, the legend is populated by calling \ref QCPAbstractPlottable::addToLegend. The + respective legend item can be removed with \ref QCPAbstractPlottable::removeFromLegend. However, + QCPLegend also offers an interface to add and manipulate legend items directly: \ref item, \ref + itemWithPlottable, \ref itemCount, \ref addItem, \ref removeItem, etc. + + The QCPLegend derives from QCPLayoutGrid and as such can be placed in any position a + QCPLayoutElement may be positioned. The legend items are themselves QCPLayoutElements which are + placed in the grid layout of the legend. QCPLegend only adds an interface specialized for + handling child elements of type QCPAbstractLegendItem, as mentioned above. In principle, any + other layout elements may also be added to a legend via the normal \ref QCPLayoutGrid interface. + However, the QCPAbstractLegendItem-Interface will ignore those elements (e.g. \ref itemCount will + only return the number of items with QCPAbstractLegendItems type). + + By default, every QCustomPlot has one legend (QCustomPlot::legend) which is placed in the inset + layout of the main axis rect (\ref QCPAxisRect::insetLayout). To move the legend to another + position inside the axis rect, use the methods of the \ref QCPLayoutInset. To move the legend + outside of the axis rect, place it anywhere else with the QCPLayout/QCPLayoutElement interface. +*/ + +/* start of documentation of signals */ + +/*! \fn void QCPLegend::selectionChanged(QCPLegend::SelectableParts selection); + + This signal is emitted when the selection state of this legend has changed. + + \see setSelectedParts, setSelectableParts +*/ + +/* end of documentation of signals */ + +/*! + Constructs a new QCPLegend instance with \a parentPlot as the containing plot and default values. + + Note that by default, QCustomPlot already contains a legend ready to be used as + QCustomPlot::legend +*/ +QCPLegend::QCPLegend() +{ + setRowSpacing(0); + setColumnSpacing(10); + setMargins(QMargins(2, 3, 2, 2)); + setAntialiased(false); + setIconSize(32, 18); + + setIconTextPadding(7); + + setSelectableParts(spLegendBox | spItems); + setSelectedParts(spNone); + + setBorderPen(QPen(Qt::black)); + setSelectedBorderPen(QPen(Qt::blue, 2)); + setIconBorderPen(Qt::NoPen); + setSelectedIconBorderPen(QPen(Qt::blue, 2)); + setBrush(Qt::white); + setSelectedBrush(Qt::white); + setTextColor(Qt::black); + setSelectedTextColor(Qt::blue); +} + +QCPLegend::~QCPLegend() +{ + clearItems(); + if (qobject_cast(mParentPlot)) // make sure this isn't called from QObject dtor when QCustomPlot is already destructed (happens when the legend is not in any layout and thus QObject-child of QCustomPlot) + mParentPlot->legendRemoved(this); +} + +/* no doc for getter, see setSelectedParts */ +QCPLegend::SelectableParts QCPLegend::selectedParts() const +{ + // check whether any legend elements selected, if yes, add spItems to return value + bool hasSelectedItems = false; + for (int i=0; iselected()) + { + hasSelectedItems = true; + break; + } + } + if (hasSelectedItems) + return mSelectedParts | spItems; + else + return mSelectedParts & ~spItems; +} + +/*! + Sets the pen, the border of the entire legend is drawn with. +*/ +void QCPLegend::setBorderPen(const QPen &pen) +{ + mBorderPen = pen; +} + +/*! + Sets the brush of the legend background. +*/ +void QCPLegend::setBrush(const QBrush &brush) +{ + mBrush = brush; +} + +/*! + Sets the default font of legend text. Legend items that draw text (e.g. the name of a graph) will + use this font by default. However, a different font can be specified on a per-item-basis by + accessing the specific legend item. + + This function will also set \a font on all already existing legend items. + + \see QCPAbstractLegendItem::setFont +*/ +void QCPLegend::setFont(const QFont &font) +{ + mFont = font; + for (int i=0; isetFont(mFont); + } +} + +/*! + Sets the default color of legend text. Legend items that draw text (e.g. the name of a graph) + will use this color by default. However, a different colors can be specified on a per-item-basis + by accessing the specific legend item. + + This function will also set \a color on all already existing legend items. + + \see QCPAbstractLegendItem::setTextColor +*/ +void QCPLegend::setTextColor(const QColor &color) +{ + mTextColor = color; + for (int i=0; isetTextColor(color); + } +} + +/*! + Sets the size of legend icons. Legend items that draw an icon (e.g. a visual + representation of the graph) will use this size by default. +*/ +void QCPLegend::setIconSize(const QSize &size) +{ + mIconSize = size; +} + +/*! \overload +*/ +void QCPLegend::setIconSize(int width, int height) +{ + mIconSize.setWidth(width); + mIconSize.setHeight(height); +} + +/*! + Sets the horizontal space in pixels between the legend icon and the text next to it. + Legend items that draw an icon (e.g. a visual representation of the graph) and text (e.g. the + name of the graph) will use this space by default. +*/ +void QCPLegend::setIconTextPadding(int padding) +{ + mIconTextPadding = padding; +} + +/*! + Sets the pen used to draw a border around each legend icon. Legend items that draw an + icon (e.g. a visual representation of the graph) will use this pen by default. + + If no border is wanted, set this to \a Qt::NoPen. +*/ +void QCPLegend::setIconBorderPen(const QPen &pen) +{ + mIconBorderPen = pen; +} + +/*! + Sets whether the user can (de-)select the parts in \a selectable by clicking on the QCustomPlot surface. + (When \ref QCustomPlot::setInteractions contains \ref QCP::iSelectLegend.) + + However, even when \a selectable is set to a value not allowing the selection of a specific part, + it is still possible to set the selection of this part manually, by calling \ref setSelectedParts + directly. + + \see SelectablePart, setSelectedParts +*/ +void QCPLegend::setSelectableParts(const SelectableParts &selectable) +{ + if (mSelectableParts != selectable) + { + mSelectableParts = selectable; + emit selectableChanged(mSelectableParts); + } +} + +/*! + Sets the selected state of the respective legend parts described by \ref SelectablePart. When a part + is selected, it uses a different pen/font and brush. If some legend items are selected and \a selected + doesn't contain \ref spItems, those items become deselected. + + The entire selection mechanism is handled automatically when \ref QCustomPlot::setInteractions + contains iSelectLegend. You only need to call this function when you wish to change the selection + state manually. + + This function can change the selection state of a part even when \ref setSelectableParts was set to a + value that actually excludes the part. + + emits the \ref selectionChanged signal when \a selected is different from the previous selection state. + + Note that it doesn't make sense to set the selected state \ref spItems here when it wasn't set + before, because there's no way to specify which exact items to newly select. Do this by calling + \ref QCPAbstractLegendItem::setSelected directly on the legend item you wish to select. + + \see SelectablePart, setSelectableParts, selectTest, setSelectedBorderPen, setSelectedIconBorderPen, setSelectedBrush, + setSelectedFont +*/ +void QCPLegend::setSelectedParts(const SelectableParts &selected) +{ + SelectableParts newSelected = selected; + mSelectedParts = this->selectedParts(); // update mSelectedParts in case item selection changed + + if (mSelectedParts != newSelected) + { + if (!mSelectedParts.testFlag(spItems) && newSelected.testFlag(spItems)) // attempt to set spItems flag (can't do that) + { + qDebug() << Q_FUNC_INFO << "spItems flag can not be set, it can only be unset with this function"; + newSelected &= ~spItems; + } + if (mSelectedParts.testFlag(spItems) && !newSelected.testFlag(spItems)) // spItems flag was unset, so clear item selection + { + for (int i=0; isetSelected(false); + } + } + mSelectedParts = newSelected; + emit selectionChanged(mSelectedParts); + } +} + +/*! + When the legend box is selected, this pen is used to draw the border instead of the normal pen + set via \ref setBorderPen. + + \see setSelectedParts, setSelectableParts, setSelectedBrush +*/ +void QCPLegend::setSelectedBorderPen(const QPen &pen) +{ + mSelectedBorderPen = pen; +} + +/*! + Sets the pen legend items will use to draw their icon borders, when they are selected. + + \see setSelectedParts, setSelectableParts, setSelectedFont +*/ +void QCPLegend::setSelectedIconBorderPen(const QPen &pen) +{ + mSelectedIconBorderPen = pen; +} + +/*! + When the legend box is selected, this brush is used to draw the legend background instead of the normal brush + set via \ref setBrush. + + \see setSelectedParts, setSelectableParts, setSelectedBorderPen +*/ +void QCPLegend::setSelectedBrush(const QBrush &brush) +{ + mSelectedBrush = brush; +} + +/*! + Sets the default font that is used by legend items when they are selected. + + This function will also set \a font on all already existing legend items. + + \see setFont, QCPAbstractLegendItem::setSelectedFont +*/ +void QCPLegend::setSelectedFont(const QFont &font) +{ + mSelectedFont = font; + for (int i=0; isetSelectedFont(font); + } +} + +/*! + Sets the default text color that is used by legend items when they are selected. + + This function will also set \a color on all already existing legend items. + + \see setTextColor, QCPAbstractLegendItem::setSelectedTextColor +*/ +void QCPLegend::setSelectedTextColor(const QColor &color) +{ + mSelectedTextColor = color; + for (int i=0; isetSelectedTextColor(color); + } +} + +/*! + Returns the item with index \a i. + + \see itemCount +*/ +QCPAbstractLegendItem *QCPLegend::item(int index) const +{ + return qobject_cast(elementAt(index)); +} + +/*! + Returns the QCPPlottableLegendItem which is associated with \a plottable (e.g. a \ref QCPGraph*). + If such an item isn't in the legend, returns 0. + + \see hasItemWithPlottable +*/ +QCPPlottableLegendItem *QCPLegend::itemWithPlottable(const QCPAbstractPlottable *plottable) const +{ + for (int i=0; i(item(i))) + { + if (pli->plottable() == plottable) + return pli; + } + } + return 0; +} + +/*! + Returns the number of items currently in the legend. + \see item +*/ +int QCPLegend::itemCount() const +{ + return elementCount(); +} + +/*! + Returns whether the legend contains \a itm. +*/ +bool QCPLegend::hasItem(QCPAbstractLegendItem *item) const +{ + for (int i=0; iitem(i)) + return true; + } + return false; +} + +/*! + Returns whether the legend contains a QCPPlottableLegendItem which is associated with \a plottable (e.g. a \ref QCPGraph*). + If such an item isn't in the legend, returns false. + + \see itemWithPlottable +*/ +bool QCPLegend::hasItemWithPlottable(const QCPAbstractPlottable *plottable) const +{ + return itemWithPlottable(plottable); +} + +/*! + Adds \a item to the legend, if it's not present already. + + Returns true on sucess, i.e. if the item wasn't in the list already and has been successfuly added. + + The legend takes ownership of the item. +*/ +bool QCPLegend::addItem(QCPAbstractLegendItem *item) +{ + if (!hasItem(item)) + { + return addElement(rowCount(), 0, item); + } else + return false; +} + +/*! + Removes the item with index \a index from the legend. + + Returns true, if successful. + + \see itemCount, clearItems +*/ +bool QCPLegend::removeItem(int index) +{ + if (QCPAbstractLegendItem *ali = item(index)) + { + bool success = remove(ali); + simplify(); + return success; + } else + return false; +} + +/*! \overload + + Removes \a item from the legend. + + Returns true, if successful. + + \see clearItems +*/ +bool QCPLegend::removeItem(QCPAbstractLegendItem *item) +{ + bool success = remove(item); + simplify(); + return success; +} + +/*! + Removes all items from the legend. +*/ +void QCPLegend::clearItems() +{ + for (int i=itemCount()-1; i>=0; --i) + removeItem(i); +} + +/*! + Returns the legend items that are currently selected. If no items are selected, + the list is empty. + + \see QCPAbstractLegendItem::setSelected, setSelectable +*/ +QList QCPLegend::selectedItems() const +{ + QList result; + for (int i=0; iselected()) + result.append(ali); + } + } + return result; +} + +/*! \internal + + A convenience function to easily set the QPainter::Antialiased hint on the provided \a painter + before drawing main legend elements. + + This is the antialiasing state the painter passed to the \ref draw method is in by default. + + This function takes into account the local setting of the antialiasing flag as well as the + overrides set with \ref QCustomPlot::setAntialiasedElements and \ref + QCustomPlot::setNotAntialiasedElements. + + \see setAntialiased +*/ +void QCPLegend::applyDefaultAntialiasingHint(QCPPainter *painter) const +{ + applyAntialiasingHint(painter, mAntialiased, QCP::aeLegend); +} + +/*! \internal + + Returns the pen used to paint the border of the legend, taking into account the selection state + of the legend box. +*/ +QPen QCPLegend::getBorderPen() const +{ + return mSelectedParts.testFlag(spLegendBox) ? mSelectedBorderPen : mBorderPen; +} + +/*! \internal + + Returns the brush used to paint the background of the legend, taking into account the selection + state of the legend box. +*/ +QBrush QCPLegend::getBrush() const +{ + return mSelectedParts.testFlag(spLegendBox) ? mSelectedBrush : mBrush; +} + +/*! \internal + + Draws the legend box with the provided \a painter. The individual legend items are layerables + themselves, thus are drawn independently. +*/ +void QCPLegend::draw(QCPPainter *painter) +{ + // draw background rect: + painter->setBrush(getBrush()); + painter->setPen(getBorderPen()); + painter->drawRect(mOuterRect); +} + +/* inherits documentation from base class */ +double QCPLegend::selectTest(const QPointF &pos, bool onlySelectable, QVariant *details) const +{ + if (!mParentPlot) return -1; + if (onlySelectable && !mSelectableParts.testFlag(spLegendBox)) + return -1; + + if (mOuterRect.contains(pos.toPoint())) + { + if (details) details->setValue(spLegendBox); + return mParentPlot->selectionTolerance()*0.99; + } + return -1; +} + +/* inherits documentation from base class */ +void QCPLegend::selectEvent(QMouseEvent *event, bool additive, const QVariant &details, bool *selectionStateChanged) +{ + Q_UNUSED(event) + mSelectedParts = selectedParts(); // in case item selection has changed + if (details.value() == spLegendBox && mSelectableParts.testFlag(spLegendBox)) + { + SelectableParts selBefore = mSelectedParts; + setSelectedParts(additive ? mSelectedParts^spLegendBox : mSelectedParts|spLegendBox); // no need to unset spItems in !additive case, because they will be deselected by QCustomPlot (they're normal QCPLayerables with own deselectEvent) + if (selectionStateChanged) + *selectionStateChanged = mSelectedParts != selBefore; + } +} + +/* inherits documentation from base class */ +void QCPLegend::deselectEvent(bool *selectionStateChanged) +{ + mSelectedParts = selectedParts(); // in case item selection has changed + if (mSelectableParts.testFlag(spLegendBox)) + { + SelectableParts selBefore = mSelectedParts; + setSelectedParts(selectedParts() & ~spLegendBox); + if (selectionStateChanged) + *selectionStateChanged = mSelectedParts != selBefore; + } +} + +/* inherits documentation from base class */ +QCP::Interaction QCPLegend::selectionCategory() const +{ + return QCP::iSelectLegend; +} + +/* inherits documentation from base class */ +QCP::Interaction QCPAbstractLegendItem::selectionCategory() const +{ + return QCP::iSelectLegend; +} + +/* inherits documentation from base class */ +void QCPLegend::parentPlotInitialized(QCustomPlot *parentPlot) +{ + Q_UNUSED(parentPlot) +} + + +//////////////////////////////////////////////////////////////////////////////////////////////////// +//////////////////// QCPPlotTitle +//////////////////////////////////////////////////////////////////////////////////////////////////// + +/*! \class QCPPlotTitle + \brief A layout element displaying a plot title text + + The text may be specified with \ref setText, theformatting can be controlled with \ref setFont + and \ref setTextColor. + + A plot title can be added as follows: + \snippet documentation/doc-code-snippets/mainwindow.cpp qcpplottitle-creation + + Since a plot title is a common requirement, QCustomPlot offers specialized selection signals for + easy interaction with QCPPlotTitle. If a layout element of type QCPPlotTitle is clicked, the + signal \ref QCustomPlot::titleClick is emitted. A double click emits the \ref + QCustomPlot::titleDoubleClick signal. +*/ + +/* start documentation of signals */ + +/*! \fn void QCPPlotTitle::selectionChanged(bool selected) + + This signal is emitted when the selection state has changed to \a selected, either by user + interaction or by a direct call to \ref setSelected. + + \see setSelected, setSelectable +*/ + +/* end documentation of signals */ + +/*! + Creates a new QCPPlotTitle instance and sets default values. The initial text is empty (\ref setText). + + To set the title text in the constructor, rather use \ref QCPPlotTitle(QCustomPlot *parentPlot, const QString &text). +*/ +QCPPlotTitle::QCPPlotTitle(QCustomPlot *parentPlot) : + QCPLayoutElement(parentPlot), + mFont(QFont(QLatin1String("sans serif"), 13*1.5, QFont::Bold)), + mTextColor(Qt::black), + mSelectedFont(QFont(QLatin1String("sans serif"), 13*1.6, QFont::Bold)), + mSelectedTextColor(Qt::blue), + mSelectable(false), + mSelected(false) +{ + if (parentPlot) + { + setLayer(parentPlot->currentLayer()); + mFont = QFont(parentPlot->font().family(), parentPlot->font().pointSize()*1.5, QFont::Bold); + mSelectedFont = QFont(parentPlot->font().family(), parentPlot->font().pointSize()*1.6, QFont::Bold); + } + setMargins(QMargins(5, 5, 5, 0)); +} + +/*! \overload + + Creates a new QCPPlotTitle instance and sets default values. The initial text is set to \a text. +*/ +QCPPlotTitle::QCPPlotTitle(QCustomPlot *parentPlot, const QString &text) : + QCPLayoutElement(parentPlot), + mText(text), + mFont(QFont(parentPlot->font().family(), parentPlot->font().pointSize()*1.5, QFont::Bold)), + mTextColor(Qt::black), + mSelectedFont(QFont(parentPlot->font().family(), parentPlot->font().pointSize()*1.6, QFont::Bold)), + mSelectedTextColor(Qt::blue), + mSelectable(false), + mSelected(false) +{ + setLayer(QLatin1String("axes")); + setMargins(QMargins(5, 5, 5, 0)); +} + +/*! + Sets the text that will be displayed to \a text. Multiple lines can be created by insertion of "\n". + + \see setFont, setTextColor +*/ +void QCPPlotTitle::setText(const QString &text) +{ + mText = text; +} + +/*! + Sets the \a font of the title text. + + \see setTextColor, setSelectedFont +*/ +void QCPPlotTitle::setFont(const QFont &font) +{ + mFont = font; +} + +/*! + Sets the \a color of the title text. + + \see setFont, setSelectedTextColor +*/ +void QCPPlotTitle::setTextColor(const QColor &color) +{ + mTextColor = color; +} + +/*! + Sets the \a font of the title text that will be used if the plot title is selected (\ref setSelected). + + \see setFont +*/ +void QCPPlotTitle::setSelectedFont(const QFont &font) +{ + mSelectedFont = font; +} + +/*! + Sets the \a color of the title text that will be used if the plot title is selected (\ref setSelected). + + \see setTextColor +*/ +void QCPPlotTitle::setSelectedTextColor(const QColor &color) +{ + mSelectedTextColor = color; +} + +/*! + Sets whether the user may select this plot title to \a selectable. + + Note that even when \a selectable is set to false, the selection state may be changed + programmatically via \ref setSelected. +*/ +void QCPPlotTitle::setSelectable(bool selectable) +{ + if (mSelectable != selectable) + { + mSelectable = selectable; + emit selectableChanged(mSelectable); + } +} + +/*! + Sets the selection state of this plot title to \a selected. If the selection has changed, \ref + selectionChanged is emitted. + + Note that this function can change the selection state independently of the current \ref + setSelectable state. +*/ +void QCPPlotTitle::setSelected(bool selected) +{ + if (mSelected != selected) + { + mSelected = selected; + emit selectionChanged(mSelected); + } +} + +/* inherits documentation from base class */ +void QCPPlotTitle::applyDefaultAntialiasingHint(QCPPainter *painter) const +{ + applyAntialiasingHint(painter, mAntialiased, QCP::aeNone); +} + +/* inherits documentation from base class */ +void QCPPlotTitle::draw(QCPPainter *painter) +{ + painter->setFont(mainFont()); + painter->setPen(QPen(mainTextColor())); + painter->drawText(mRect, Qt::AlignCenter, mText, &mTextBoundingRect); +} + +/* inherits documentation from base class */ +QSize QCPPlotTitle::minimumSizeHint() const +{ + QFontMetrics metrics(mFont); + QSize result = metrics.boundingRect(0, 0, 0, 0, Qt::AlignCenter, mText).size(); + result.rwidth() += mMargins.left() + mMargins.right(); + result.rheight() += mMargins.top() + mMargins.bottom(); + return result; +} + +/* inherits documentation from base class */ +QSize QCPPlotTitle::maximumSizeHint() const +{ + QFontMetrics metrics(mFont); + QSize result = metrics.boundingRect(0, 0, 0, 0, Qt::AlignCenter, mText).size(); + result.rheight() += mMargins.top() + mMargins.bottom(); + result.setWidth(QWIDGETSIZE_MAX); + return result; +} + +/* inherits documentation from base class */ +void QCPPlotTitle::selectEvent(QMouseEvent *event, bool additive, const QVariant &details, bool *selectionStateChanged) +{ + Q_UNUSED(event) + Q_UNUSED(details) + if (mSelectable) + { + bool selBefore = mSelected; + setSelected(additive ? !mSelected : true); + if (selectionStateChanged) + *selectionStateChanged = mSelected != selBefore; + } +} + +/* inherits documentation from base class */ +void QCPPlotTitle::deselectEvent(bool *selectionStateChanged) +{ + if (mSelectable) + { + bool selBefore = mSelected; + setSelected(false); + if (selectionStateChanged) + *selectionStateChanged = mSelected != selBefore; + } +} + +/* inherits documentation from base class */ +double QCPPlotTitle::selectTest(const QPointF &pos, bool onlySelectable, QVariant *details) const +{ + Q_UNUSED(details) + if (onlySelectable && !mSelectable) + return -1; + + if (mTextBoundingRect.contains(pos.toPoint())) + return mParentPlot->selectionTolerance()*0.99; + else + return -1; +} + +/*! \internal + + Returns the main font to be used. This is mSelectedFont if \ref setSelected is set to + true, else mFont is returned. +*/ +QFont QCPPlotTitle::mainFont() const +{ + return mSelected ? mSelectedFont : mFont; +} + +/*! \internal + + Returns the main color to be used. This is mSelectedTextColor if \ref setSelected is set to + true, else mTextColor is returned. +*/ +QColor QCPPlotTitle::mainTextColor() const +{ + return mSelected ? mSelectedTextColor : mTextColor; +} + + +//////////////////////////////////////////////////////////////////////////////////////////////////// +//////////////////// QCPColorScale +//////////////////////////////////////////////////////////////////////////////////////////////////// + +/*! \class QCPColorScale + \brief A color scale for use with color coding data such as QCPColorMap + + This layout element can be placed on the plot to correlate a color gradient with data values. It + is usually used in combination with one or multiple \ref QCPColorMap "QCPColorMaps". + + \image html QCPColorScale.png + + The color scale can be either horizontal or vertical, as shown in the image above. The + orientation and the side where the numbers appear is controlled with \ref setType. + + Use \ref QCPColorMap::setColorScale to connect a color map with a color scale. Once they are + connected, they share their gradient, data range and data scale type (\ref setGradient, \ref + setDataRange, \ref setDataScaleType). Multiple color maps may be associated with a single color + scale, to make them all synchronize these properties. + + To have finer control over the number display and axis behaviour, you can directly access the + \ref axis. See the documentation of QCPAxis for details about configuring axes. For example, if + you want to change the number of automatically generated ticks, call + \snippet documentation/doc-code-snippets/mainwindow.cpp qcpcolorscale-autotickcount + + Placing a color scale next to the main axis rect works like with any other layout element: + \snippet documentation/doc-code-snippets/mainwindow.cpp qcpcolorscale-creation + In this case we have placed it to the right of the default axis rect, so it wasn't necessary to + call \ref setType, since \ref QCPAxis::atRight is already the default. The text next to the color + scale can be set with \ref setLabel. + + For optimum appearance (like in the image above), it may be desirable to line up the axis rect and + the borders of the color scale. Use a \ref QCPMarginGroup to achieve this: + \snippet documentation/doc-code-snippets/mainwindow.cpp qcpcolorscale-margingroup + + Color scales are initialized with a non-zero minimum top and bottom margin (\ref + setMinimumMargins), because vertical color scales are most common and the minimum top/bottom + margin makes sure it keeps some distance to the top/bottom widget border. So if you change to a + horizontal color scale by setting \ref setType to \ref QCPAxis::atBottom or \ref QCPAxis::atTop, you + might want to also change the minimum margins accordingly, e.g. setMinimumMargins(QMargins(6, 0, 6, 0)). +*/ + +/* start documentation of inline functions */ + +/*! \fn QCPAxis *QCPColorScale::axis() const + + Returns the internal \ref QCPAxis instance of this color scale. You can access it to alter the + appearance and behaviour of the axis. \ref QCPColorScale duplicates some properties in its + interface for convenience. Those are \ref setDataRange (\ref QCPAxis::setRange), \ref + setDataScaleType (\ref QCPAxis::setScaleType), and the method \ref setLabel (\ref + QCPAxis::setLabel). As they each are connected, it does not matter whether you use the method on + the QCPColorScale or on its QCPAxis. + + If the type of the color scale is changed with \ref setType, the axis returned by this method + will change, too, to either the left, right, bottom or top axis, depending on which type was set. +*/ + +/* end documentation of signals */ +/* start documentation of signals */ + +/*! \fn void QCPColorScale::dataRangeChanged(QCPRange newRange); + + This signal is emitted when the data range changes. + + \see setDataRange +*/ + +/*! \fn void QCPColorScale::dataScaleTypeChanged(QCPAxis::ScaleType scaleType); + + This signal is emitted when the data scale type changes. + + \see setDataScaleType +*/ + +/*! \fn void QCPColorScale::gradientChanged(QCPColorGradient newGradient); + + This signal is emitted when the gradient changes. + + \see setGradient +*/ + +/* end documentation of signals */ + +/*! + Constructs a new QCPColorScale. +*/ +QCPColorScale::QCPColorScale(QCustomPlot *parentPlot) : + QCPLayoutElement(parentPlot), + mType(QCPAxis::atTop), // set to atTop such that setType(QCPAxis::atRight) below doesn't skip work because it thinks it's already atRight + mDataScaleType(QCPAxis::stLinear), + mBarWidth(20), + mAxisRect(new QCPColorScaleAxisRectPrivate(this)) +{ + setMinimumMargins(QMargins(0, 6, 0, 6)); // for default right color scale types, keep some room at bottom and top (important if no margin group is used) + setType(QCPAxis::atRight); + setDataRange(QCPRange(0, 6)); +} + +QCPColorScale::~QCPColorScale() +{ + delete mAxisRect; +} + +/* undocumented getter */ +QString QCPColorScale::label() const +{ + if (!mColorAxis) + { + qDebug() << Q_FUNC_INFO << "internal color axis undefined"; + return QString(); + } + + return mColorAxis.data()->label(); +} + +/* undocumented getter */ +bool QCPColorScale::rangeDrag() const +{ + if (!mAxisRect) + { + qDebug() << Q_FUNC_INFO << "internal axis rect was deleted"; + return false; + } + + return mAxisRect.data()->rangeDrag().testFlag(QCPAxis::orientation(mType)) && + mAxisRect.data()->rangeDragAxis(QCPAxis::orientation(mType)) && + mAxisRect.data()->rangeDragAxis(QCPAxis::orientation(mType))->orientation() == QCPAxis::orientation(mType); +} + +/* undocumented getter */ +bool QCPColorScale::rangeZoom() const +{ + if (!mAxisRect) + { + qDebug() << Q_FUNC_INFO << "internal axis rect was deleted"; + return false; + } + + return mAxisRect.data()->rangeZoom().testFlag(QCPAxis::orientation(mType)) && + mAxisRect.data()->rangeZoomAxis(QCPAxis::orientation(mType)) && + mAxisRect.data()->rangeZoomAxis(QCPAxis::orientation(mType))->orientation() == QCPAxis::orientation(mType); +} + +/*! + Sets at which side of the color scale the axis is placed, and thus also its orientation. + + Note that after setting \a type to a different value, the axis returned by \ref axis() will + be a different one. The new axis will adopt the following properties from the previous axis: The + range, scale type, log base and label. +*/ +void QCPColorScale::setType(QCPAxis::AxisType type) +{ + if (!mAxisRect) + { + qDebug() << Q_FUNC_INFO << "internal axis rect was deleted"; + return; + } + if (mType != type) + { + mType = type; + QCPRange rangeTransfer(0, 6); + double logBaseTransfer = 10; + QString labelTransfer; + // revert some settings on old axis: + if (mColorAxis) + { + rangeTransfer = mColorAxis.data()->range(); + labelTransfer = mColorAxis.data()->label(); + logBaseTransfer = mColorAxis.data()->scaleLogBase(); + mColorAxis.data()->setLabel(QString()); + disconnect(mColorAxis.data(), SIGNAL(rangeChanged(QCPRange)), this, SLOT(setDataRange(QCPRange))); + disconnect(mColorAxis.data(), SIGNAL(scaleTypeChanged(QCPAxis::ScaleType)), this, SLOT(setDataScaleType(QCPAxis::ScaleType))); + } + QList allAxisTypes = QList() << QCPAxis::atLeft << QCPAxis::atRight << QCPAxis::atBottom << QCPAxis::atTop; + foreach (QCPAxis::AxisType atype, allAxisTypes) + { + mAxisRect.data()->axis(atype)->setTicks(atype == mType); + mAxisRect.data()->axis(atype)->setTickLabels(atype== mType); + } + // set new mColorAxis pointer: + mColorAxis = mAxisRect.data()->axis(mType); + // transfer settings to new axis: + mColorAxis.data()->setRange(rangeTransfer); // transfer range of old axis to new one (necessary if axis changes from vertical to horizontal or vice versa) + mColorAxis.data()->setLabel(labelTransfer); + mColorAxis.data()->setScaleLogBase(logBaseTransfer); // scaleType is synchronized among axes in realtime via signals (connected in QCPColorScale ctor), so we only need to take care of log base here + connect(mColorAxis.data(), SIGNAL(rangeChanged(QCPRange)), this, SLOT(setDataRange(QCPRange))); + connect(mColorAxis.data(), SIGNAL(scaleTypeChanged(QCPAxis::ScaleType)), this, SLOT(setDataScaleType(QCPAxis::ScaleType))); + mAxisRect.data()->setRangeDragAxes(QCPAxis::orientation(mType) == Qt::Horizontal ? mColorAxis.data() : 0, + QCPAxis::orientation(mType) == Qt::Vertical ? mColorAxis.data() : 0); + } +} + +/*! + Sets the range spanned by the color gradient and that is shown by the axis in the color scale. + + It is equivalent to calling QCPColorMap::setDataRange on any of the connected color maps. It is + also equivalent to directly accessing the \ref axis and setting its range with \ref + QCPAxis::setRange. + + \see setDataScaleType, setGradient, rescaleDataRange +*/ +void QCPColorScale::setDataRange(const QCPRange &dataRange) +{ + if (mDataRange.lower != dataRange.lower || mDataRange.upper != dataRange.upper) + { + mDataRange = dataRange; + if (mColorAxis) + mColorAxis.data()->setRange(mDataRange); + emit dataRangeChanged(mDataRange); + } +} + +/*! + Sets the scale type of the color scale, i.e. whether values are linearly associated with colors + or logarithmically. + + It is equivalent to calling QCPColorMap::setDataScaleType on any of the connected color maps. It is + also equivalent to directly accessing the \ref axis and setting its scale type with \ref + QCPAxis::setScaleType. + + \see setDataRange, setGradient +*/ +void QCPColorScale::setDataScaleType(QCPAxis::ScaleType scaleType) +{ + if (mDataScaleType != scaleType) + { + mDataScaleType = scaleType; + if (mColorAxis) + mColorAxis.data()->setScaleType(mDataScaleType); + if (mDataScaleType == QCPAxis::stLogarithmic) + setDataRange(mDataRange.sanitizedForLogScale()); + emit dataScaleTypeChanged(mDataScaleType); + } +} + +/*! + Sets the color gradient that will be used to represent data values. + + It is equivalent to calling QCPColorMap::setGradient on any of the connected color maps. + + \see setDataRange, setDataScaleType +*/ +void QCPColorScale::setGradient(const QCPColorGradient &gradient) +{ + if (mGradient != gradient) + { + mGradient = gradient; + if (mAxisRect) + mAxisRect.data()->mGradientImageInvalidated = true; + emit gradientChanged(mGradient); + } +} + +/*! + Sets the axis label of the color scale. This is equivalent to calling \ref QCPAxis::setLabel on + the internal \ref axis. +*/ +void QCPColorScale::setLabel(const QString &str) +{ + if (!mColorAxis) + { + qDebug() << Q_FUNC_INFO << "internal color axis undefined"; + return; + } + + mColorAxis.data()->setLabel(str); +} + +/*! + Sets the width (or height, for horizontal color scales) the bar where the gradient is displayed + will have. +*/ +void QCPColorScale::setBarWidth(int width) +{ + mBarWidth = width; +} + +/*! + Sets whether the user can drag the data range (\ref setDataRange). + + Note that \ref QCP::iRangeDrag must be in the QCustomPlot's interactions (\ref + QCustomPlot::setInteractions) to allow range dragging. +*/ +void QCPColorScale::setRangeDrag(bool enabled) +{ + if (!mAxisRect) + { + qDebug() << Q_FUNC_INFO << "internal axis rect was deleted"; + return; + } + + if (enabled) + mAxisRect.data()->setRangeDrag(QCPAxis::orientation(mType)); + else + mAxisRect.data()->setRangeDrag(0); +} + +/*! + Sets whether the user can zoom the data range (\ref setDataRange) by scrolling the mouse wheel. + + Note that \ref QCP::iRangeZoom must be in the QCustomPlot's interactions (\ref + QCustomPlot::setInteractions) to allow range dragging. +*/ +void QCPColorScale::setRangeZoom(bool enabled) +{ + if (!mAxisRect) + { + qDebug() << Q_FUNC_INFO << "internal axis rect was deleted"; + return; + } + + if (enabled) + mAxisRect.data()->setRangeZoom(QCPAxis::orientation(mType)); + else + mAxisRect.data()->setRangeZoom(0); +} + +/*! + Returns a list of all the color maps associated with this color scale. +*/ +QList QCPColorScale::colorMaps() const +{ + QList result; + for (int i=0; iplottableCount(); ++i) + { + if (QCPColorMap *cm = qobject_cast(mParentPlot->plottable(i))) + if (cm->colorScale() == this) + result.append(cm); + } + return result; +} + +/*! + Changes the data range such that all color maps associated with this color scale are fully mapped + to the gradient in the data dimension. + + \see setDataRange +*/ +void QCPColorScale::rescaleDataRange(bool onlyVisibleMaps) +{ + QList maps = colorMaps(); + QCPRange newRange; + bool haveRange = false; + int sign = 0; // TODO: should change this to QCPAbstractPlottable::SignDomain later (currently is protected, maybe move to QCP namespace) + if (mDataScaleType == QCPAxis::stLogarithmic) + sign = (mDataRange.upper < 0 ? -1 : 1); + for (int i=0; irealVisibility() && onlyVisibleMaps) + continue; + QCPRange mapRange; + if (maps.at(i)->colorScale() == this) + { + bool currentFoundRange = true; + mapRange = maps.at(i)->data()->dataBounds(); + if (sign == 1) + { + if (mapRange.lower <= 0 && mapRange.upper > 0) + mapRange.lower = mapRange.upper*1e-3; + else if (mapRange.lower <= 0 && mapRange.upper <= 0) + currentFoundRange = false; + } else if (sign == -1) + { + if (mapRange.upper >= 0 && mapRange.lower < 0) + mapRange.upper = mapRange.lower*1e-3; + else if (mapRange.upper >= 0 && mapRange.lower >= 0) + currentFoundRange = false; + } + if (currentFoundRange) + { + if (!haveRange) + newRange = mapRange; + else + newRange.expand(mapRange); + haveRange = true; + } + } + } + if (haveRange) + { + if (!QCPRange::validRange(newRange)) // likely due to range being zero (plottable has only constant data in this dimension), shift current range to at least center the data + { + double center = (newRange.lower+newRange.upper)*0.5; // upper and lower should be equal anyway, but just to make sure, incase validRange returned false for other reason + if (mDataScaleType == QCPAxis::stLinear) + { + newRange.lower = center-mDataRange.size()/2.0; + newRange.upper = center+mDataRange.size()/2.0; + } else // mScaleType == stLogarithmic + { + newRange.lower = center/qSqrt(mDataRange.upper/mDataRange.lower); + newRange.upper = center*qSqrt(mDataRange.upper/mDataRange.lower); + } + } + setDataRange(newRange); + } +} + +/* inherits documentation from base class */ +void QCPColorScale::update(UpdatePhase phase) +{ + QCPLayoutElement::update(phase); + if (!mAxisRect) + { + qDebug() << Q_FUNC_INFO << "internal axis rect was deleted"; + return; + } + + mAxisRect.data()->update(phase); + + switch (phase) + { + case upMargins: + { + if (mType == QCPAxis::atBottom || mType == QCPAxis::atTop) + { + setMaximumSize(QWIDGETSIZE_MAX, mBarWidth+mAxisRect.data()->margins().top()+mAxisRect.data()->margins().bottom()+margins().top()+margins().bottom()); + setMinimumSize(0, mBarWidth+mAxisRect.data()->margins().top()+mAxisRect.data()->margins().bottom()+margins().top()+margins().bottom()); + } else + { + setMaximumSize(mBarWidth+mAxisRect.data()->margins().left()+mAxisRect.data()->margins().right()+margins().left()+margins().right(), QWIDGETSIZE_MAX); + setMinimumSize(mBarWidth+mAxisRect.data()->margins().left()+mAxisRect.data()->margins().right()+margins().left()+margins().right(), 0); + } + break; + } + case upLayout: + { + mAxisRect.data()->setOuterRect(rect()); + break; + } + default: break; + } +} + +/* inherits documentation from base class */ +void QCPColorScale::applyDefaultAntialiasingHint(QCPPainter *painter) const +{ + painter->setAntialiasing(false); +} + +/* inherits documentation from base class */ +void QCPColorScale::mousePressEvent(QMouseEvent *event) +{ + if (!mAxisRect) + { + qDebug() << Q_FUNC_INFO << "internal axis rect was deleted"; + return; + } + mAxisRect.data()->mousePressEvent(event); +} + +/* inherits documentation from base class */ +void QCPColorScale::mouseMoveEvent(QMouseEvent *event) +{ + if (!mAxisRect) + { + qDebug() << Q_FUNC_INFO << "internal axis rect was deleted"; + return; + } + mAxisRect.data()->mouseMoveEvent(event); +} + +/* inherits documentation from base class */ +void QCPColorScale::mouseReleaseEvent(QMouseEvent *event) +{ + if (!mAxisRect) + { + qDebug() << Q_FUNC_INFO << "internal axis rect was deleted"; + return; + } + mAxisRect.data()->mouseReleaseEvent(event); +} + +/* inherits documentation from base class */ +void QCPColorScale::wheelEvent(QWheelEvent *event) +{ + if (!mAxisRect) + { + qDebug() << Q_FUNC_INFO << "internal axis rect was deleted"; + return; + } + mAxisRect.data()->wheelEvent(event); +} + +//////////////////////////////////////////////////////////////////////////////////////////////////// +//////////////////// QCPColorScaleAxisRectPrivate +//////////////////////////////////////////////////////////////////////////////////////////////////// + +/*! \class QCPColorScaleAxisRectPrivate + + \internal + \brief An axis rect subclass for use in a QCPColorScale + + This is a private class and not part of the public QCustomPlot interface. + + It provides the axis rect functionality for the QCPColorScale class. +*/ + + +/*! + Creates a new instance, as a child of \a parentColorScale. +*/ +QCPColorScaleAxisRectPrivate::QCPColorScaleAxisRectPrivate(QCPColorScale *parentColorScale) : + QCPAxisRect(parentColorScale->parentPlot(), true), + mParentColorScale(parentColorScale), + mGradientImageInvalidated(true) +{ + setParentLayerable(parentColorScale); + setMinimumMargins(QMargins(0, 0, 0, 0)); + QList allAxisTypes = QList() << QCPAxis::atBottom << QCPAxis::atTop << QCPAxis::atLeft << QCPAxis::atRight; + foreach (QCPAxis::AxisType type, allAxisTypes) + { + axis(type)->setVisible(true); + axis(type)->grid()->setVisible(false); + axis(type)->setPadding(0); + connect(axis(type), SIGNAL(selectionChanged(QCPAxis::SelectableParts)), this, SLOT(axisSelectionChanged(QCPAxis::SelectableParts))); + connect(axis(type), SIGNAL(selectableChanged(QCPAxis::SelectableParts)), this, SLOT(axisSelectableChanged(QCPAxis::SelectableParts))); + } + + connect(axis(QCPAxis::atLeft), SIGNAL(rangeChanged(QCPRange)), axis(QCPAxis::atRight), SLOT(setRange(QCPRange))); + connect(axis(QCPAxis::atRight), SIGNAL(rangeChanged(QCPRange)), axis(QCPAxis::atLeft), SLOT(setRange(QCPRange))); + connect(axis(QCPAxis::atBottom), SIGNAL(rangeChanged(QCPRange)), axis(QCPAxis::atTop), SLOT(setRange(QCPRange))); + connect(axis(QCPAxis::atTop), SIGNAL(rangeChanged(QCPRange)), axis(QCPAxis::atBottom), SLOT(setRange(QCPRange))); + connect(axis(QCPAxis::atLeft), SIGNAL(scaleTypeChanged(QCPAxis::ScaleType)), axis(QCPAxis::atRight), SLOT(setScaleType(QCPAxis::ScaleType))); + connect(axis(QCPAxis::atRight), SIGNAL(scaleTypeChanged(QCPAxis::ScaleType)), axis(QCPAxis::atLeft), SLOT(setScaleType(QCPAxis::ScaleType))); + connect(axis(QCPAxis::atBottom), SIGNAL(scaleTypeChanged(QCPAxis::ScaleType)), axis(QCPAxis::atTop), SLOT(setScaleType(QCPAxis::ScaleType))); + connect(axis(QCPAxis::atTop), SIGNAL(scaleTypeChanged(QCPAxis::ScaleType)), axis(QCPAxis::atBottom), SLOT(setScaleType(QCPAxis::ScaleType))); + + // make layer transfers of color scale transfer to axis rect and axes + // the axes must be set after axis rect, such that they appear above color gradient drawn by axis rect: + connect(parentColorScale, SIGNAL(layerChanged(QCPLayer*)), this, SLOT(setLayer(QCPLayer*))); + foreach (QCPAxis::AxisType type, allAxisTypes) + connect(parentColorScale, SIGNAL(layerChanged(QCPLayer*)), axis(type), SLOT(setLayer(QCPLayer*))); +} + +/*! \internal + Updates the color gradient image if necessary, by calling \ref updateGradientImage, then draws + it. Then the axes are drawn by calling the \ref QCPAxisRect::draw base class implementation. +*/ +void QCPColorScaleAxisRectPrivate::draw(QCPPainter *painter) +{ + if (mGradientImageInvalidated) + updateGradientImage(); + + bool mirrorHorz = false; + bool mirrorVert = false; + if (mParentColorScale->mColorAxis) + { + mirrorHorz = mParentColorScale->mColorAxis.data()->rangeReversed() && (mParentColorScale->type() == QCPAxis::atBottom || mParentColorScale->type() == QCPAxis::atTop); + mirrorVert = mParentColorScale->mColorAxis.data()->rangeReversed() && (mParentColorScale->type() == QCPAxis::atLeft || mParentColorScale->type() == QCPAxis::atRight); + } + + painter->drawImage(rect().adjusted(0, -1, 0, -1), mGradientImage.mirrored(mirrorHorz, mirrorVert)); + QCPAxisRect::draw(painter); +} + +/*! \internal + + Uses the current gradient of the parent \ref QCPColorScale (specified in the constructor) to + generate a gradient image. This gradient image will be used in the \ref draw method. +*/ +void QCPColorScaleAxisRectPrivate::updateGradientImage() +{ + if (rect().isEmpty()) + return; + + int n = mParentColorScale->mGradient.levelCount(); + int w, h; + QVector data(n); + for (int i=0; imType == QCPAxis::atBottom || mParentColorScale->mType == QCPAxis::atTop) + { + w = n; + h = rect().height(); + mGradientImage = QImage(w, h, QImage::Format_RGB32); + QVector pixels; + for (int y=0; y(mGradientImage.scanLine(y))); + mParentColorScale->mGradient.colorize(data.constData(), QCPRange(0, n-1), pixels.first(), n); + for (int y=1; y(mGradientImage.scanLine(y)); + const QRgb lineColor = mParentColorScale->mGradient.color(data[h-1-y], QCPRange(0, n-1)); + for (int x=0; x allAxisTypes = QList() << QCPAxis::atBottom << QCPAxis::atTop << QCPAxis::atLeft << QCPAxis::atRight; + foreach (QCPAxis::AxisType type, allAxisTypes) + { + if (QCPAxis *senderAxis = qobject_cast(sender())) + if (senderAxis->axisType() == type) + continue; + + if (axis(type)->selectableParts().testFlag(QCPAxis::spAxis)) + { + if (selectedParts.testFlag(QCPAxis::spAxis)) + axis(type)->setSelectedParts(axis(type)->selectedParts() | QCPAxis::spAxis); + else + axis(type)->setSelectedParts(axis(type)->selectedParts() & ~QCPAxis::spAxis); + } + } +} + +/*! \internal + + This slot is connected to the selectableChanged signals of the four axes in the constructor. It + synchronizes the selectability of the axes. +*/ +void QCPColorScaleAxisRectPrivate::axisSelectableChanged(QCPAxis::SelectableParts selectableParts) +{ + // synchronize axis base selectability: + QList allAxisTypes = QList() << QCPAxis::atBottom << QCPAxis::atTop << QCPAxis::atLeft << QCPAxis::atRight; + foreach (QCPAxis::AxisType type, allAxisTypes) + { + if (QCPAxis *senderAxis = qobject_cast(sender())) + if (senderAxis->axisType() == type) + continue; + + if (axis(type)->selectableParts().testFlag(QCPAxis::spAxis)) + { + if (selectableParts.testFlag(QCPAxis::spAxis)) + axis(type)->setSelectableParts(axis(type)->selectableParts() | QCPAxis::spAxis); + else + axis(type)->setSelectableParts(axis(type)->selectableParts() & ~QCPAxis::spAxis); + } + } +} + + +//////////////////////////////////////////////////////////////////////////////////////////////////// +//////////////////// QCPData +//////////////////////////////////////////////////////////////////////////////////////////////////// + +/*! \class QCPData + \brief Holds the data of one single data point for QCPGraph. + + The container for storing multiple data points is \ref QCPDataMap. + + The stored data is: + \li \a key: coordinate on the key axis of this data point + \li \a value: coordinate on the value axis of this data point + \li \a keyErrorMinus: negative error in the key dimension (for error bars) + \li \a keyErrorPlus: positive error in the key dimension (for error bars) + \li \a valueErrorMinus: negative error in the value dimension (for error bars) + \li \a valueErrorPlus: positive error in the value dimension (for error bars) + + \see QCPDataMap +*/ + +/*! + Constructs a data point with key, value and all errors set to zero. +*/ +QCPData::QCPData() : + key(0), + value(0), + keyErrorPlus(0), + keyErrorMinus(0), + valueErrorPlus(0), + valueErrorMinus(0) +{ +} + +/*! + Constructs a data point with the specified \a key and \a value. All errors are set to zero. +*/ +QCPData::QCPData(double key, double value) : + key(key), + value(value), + keyErrorPlus(0), + keyErrorMinus(0), + valueErrorPlus(0), + valueErrorMinus(0) +{ +} + + +//////////////////////////////////////////////////////////////////////////////////////////////////// +//////////////////// QCPGraph +//////////////////////////////////////////////////////////////////////////////////////////////////// + +/*! \class QCPGraph + \brief A plottable representing a graph in a plot. + + \image html QCPGraph.png + + Usually you create new graphs by calling QCustomPlot::addGraph. The resulting instance can be + accessed via QCustomPlot::graph. + + To plot data, assign it with the \ref setData or \ref addData functions. Alternatively, you can + also access and modify the graph's data via the \ref data method, which returns a pointer to the + internal \ref QCPDataMap. + + Graphs are used to display single-valued data. Single-valued means that there should only be one + data point per unique key coordinate. In other words, the graph can't have \a loops. If you do + want to plot non-single-valued curves, rather use the QCPCurve plottable. + + Gaps in the graph line can be created by adding data points with NaN as value + (qQNaN() or std::numeric_limits::quiet_NaN()) in between the two data points that shall be + separated. + + \section appearance Changing the appearance + + The appearance of the graph is mainly determined by the line style, scatter style, brush and pen + of the graph (\ref setLineStyle, \ref setScatterStyle, \ref setBrush, \ref setPen). + + \subsection filling Filling under or between graphs + + QCPGraph knows two types of fills: Normal graph fills towards the zero-value-line parallel to + the key axis of the graph, and fills between two graphs, called channel fills. To enable a fill, + just set a brush with \ref setBrush which is neither Qt::NoBrush nor fully transparent. + + By default, a normal fill towards the zero-value-line will be drawn. To set up a channel fill + between this graph and another one, call \ref setChannelFillGraph with the other graph as + parameter. + + \see QCustomPlot::addGraph, QCustomPlot::graph +*/ + +/* start of documentation of inline functions */ + +/*! \fn QCPDataMap *QCPGraph::data() const + + Returns a pointer to the internal data storage of type \ref QCPDataMap. You may use it to + directly manipulate the data, which may be more convenient and faster than using the regular \ref + setData or \ref addData methods, in certain situations. +*/ + +/* end of documentation of inline functions */ + +/*! + Constructs a graph which uses \a keyAxis as its key axis ("x") and \a valueAxis as its value + axis ("y"). \a keyAxis and \a valueAxis must reside in the same QCustomPlot instance and not have + the same orientation. If either of these restrictions is violated, a corresponding message is + printed to the debug output (qDebug), the construction is not aborted, though. + + The constructed QCPGraph can be added to the plot with QCustomPlot::addPlottable, QCustomPlot + then takes ownership of the graph. + + To directly create a graph inside a plot, you can also use the simpler QCustomPlot::addGraph function. +*/ +QCPGraph::QCPGraph(QCPAxis *keyAxis, QCPAxis *valueAxis) : + QCPAbstractPlottable(keyAxis, valueAxis) +{ + mData = new QCPDataMap; + + setPen(QPen(Qt::blue, 0)); + setErrorPen(QPen(Qt::black)); + setBrush(Qt::NoBrush); + setSelectedPen(QPen(QColor(80, 80, 255), 2.5)); + setSelectedBrush(Qt::NoBrush); + + setLineStyle(lsLine); + setErrorType(etNone); + setErrorBarSize(6); + setErrorBarSkipSymbol(true); + setChannelFillGraph(0); + setAdaptiveSampling(true); +} + +QCPGraph::~QCPGraph() +{ + delete mData; +} + +/*! + Replaces the current data with the provided \a data. + + If \a copy is set to true, data points in \a data will only be copied. if false, the graph + takes ownership of the passed data and replaces the internal data pointer with it. This is + significantly faster than copying for large datasets. + + Alternatively, you can also access and modify the graph's data via the \ref data method, which + returns a pointer to the internal \ref QCPDataMap. +*/ +void QCPGraph::setData(QCPDataMap *data, bool copy) +{ + if (mData == data) + { + qDebug() << Q_FUNC_INFO << "The data pointer is already in (and owned by) this plottable" << reinterpret_cast(data); + return; + } + if (copy) + { + *mData = *data; + } else + { + delete mData; + mData = data; + } +} + +/*! \overload + + Replaces the current data with the provided points in \a key and \a value pairs. The provided + vectors should have equal length. Else, the number of added points will be the size of the + smallest vector. +*/ +void QCPGraph::setData(const QVector &key, const QVector &value) +{ + mData->clear(); + int n = key.size(); + n = qMin(n, value.size()); + QCPData newData; + for (int i=0; iinsertMulti(newData.key, newData); + } +} + +/*! + Replaces the current data with the provided points in \a key and \a value pairs. Additionally the + symmetrical value error of the data points are set to the values in \a valueError. + For error bars to show appropriately, see \ref setErrorType. + The provided vectors should have equal length. Else, the number of added points will be the size of the + smallest vector. + + For asymmetrical errors (plus different from minus), see the overloaded version of this function. +*/ +void QCPGraph::setDataValueError(const QVector &key, const QVector &value, const QVector &valueError) +{ + mData->clear(); + int n = key.size(); + n = qMin(n, value.size()); + n = qMin(n, valueError.size()); + QCPData newData; + for (int i=0; iinsertMulti(key[i], newData); + } +} + +/*! + \overload + Replaces the current data with the provided points in \a key and \a value pairs. Additionally the + negative value error of the data points are set to the values in \a valueErrorMinus, the positive + value error to \a valueErrorPlus. + For error bars to show appropriately, see \ref setErrorType. + The provided vectors should have equal length. Else, the number of added points will be the size of the + smallest vector. +*/ +void QCPGraph::setDataValueError(const QVector &key, const QVector &value, const QVector &valueErrorMinus, const QVector &valueErrorPlus) +{ + mData->clear(); + int n = key.size(); + n = qMin(n, value.size()); + n = qMin(n, valueErrorMinus.size()); + n = qMin(n, valueErrorPlus.size()); + QCPData newData; + for (int i=0; iinsertMulti(key[i], newData); + } +} + +/*! + Replaces the current data with the provided points in \a key and \a value pairs. Additionally the + symmetrical key error of the data points are set to the values in \a keyError. + For error bars to show appropriately, see \ref setErrorType. + The provided vectors should have equal length. Else, the number of added points will be the size of the + smallest vector. + + For asymmetrical errors (plus different from minus), see the overloaded version of this function. +*/ +void QCPGraph::setDataKeyError(const QVector &key, const QVector &value, const QVector &keyError) +{ + mData->clear(); + int n = key.size(); + n = qMin(n, value.size()); + n = qMin(n, keyError.size()); + QCPData newData; + for (int i=0; iinsertMulti(key[i], newData); + } +} + +/*! + \overload + Replaces the current data with the provided points in \a key and \a value pairs. Additionally the + negative key error of the data points are set to the values in \a keyErrorMinus, the positive + key error to \a keyErrorPlus. + For error bars to show appropriately, see \ref setErrorType. + The provided vectors should have equal length. Else, the number of added points will be the size of the + smallest vector. +*/ +void QCPGraph::setDataKeyError(const QVector &key, const QVector &value, const QVector &keyErrorMinus, const QVector &keyErrorPlus) +{ + mData->clear(); + int n = key.size(); + n = qMin(n, value.size()); + n = qMin(n, keyErrorMinus.size()); + n = qMin(n, keyErrorPlus.size()); + QCPData newData; + for (int i=0; iinsertMulti(key[i], newData); + } +} + +/*! + Replaces the current data with the provided points in \a key and \a value pairs. Additionally the + symmetrical key and value errors of the data points are set to the values in \a keyError and \a valueError. + For error bars to show appropriately, see \ref setErrorType. + The provided vectors should have equal length. Else, the number of added points will be the size of the + smallest vector. + + For asymmetrical errors (plus different from minus), see the overloaded version of this function. +*/ +void QCPGraph::setDataBothError(const QVector &key, const QVector &value, const QVector &keyError, const QVector &valueError) +{ + mData->clear(); + int n = key.size(); + n = qMin(n, value.size()); + n = qMin(n, valueError.size()); + n = qMin(n, keyError.size()); + QCPData newData; + for (int i=0; iinsertMulti(key[i], newData); + } +} + +/*! + \overload + Replaces the current data with the provided points in \a key and \a value pairs. Additionally the + negative key and value errors of the data points are set to the values in \a keyErrorMinus and \a valueErrorMinus. The positive + key and value errors are set to the values in \a keyErrorPlus \a valueErrorPlus. + For error bars to show appropriately, see \ref setErrorType. + The provided vectors should have equal length. Else, the number of added points will be the size of the + smallest vector. +*/ +void QCPGraph::setDataBothError(const QVector &key, const QVector &value, const QVector &keyErrorMinus, const QVector &keyErrorPlus, const QVector &valueErrorMinus, const QVector &valueErrorPlus) +{ + mData->clear(); + int n = key.size(); + n = qMin(n, value.size()); + n = qMin(n, valueErrorMinus.size()); + n = qMin(n, valueErrorPlus.size()); + n = qMin(n, keyErrorMinus.size()); + n = qMin(n, keyErrorPlus.size()); + QCPData newData; + for (int i=0; iinsertMulti(key[i], newData); + } +} + + +/*! + Sets how the single data points are connected in the plot. For scatter-only plots, set \a ls to + \ref lsNone and \ref setScatterStyle to the desired scatter style. + + \see setScatterStyle +*/ +void QCPGraph::setLineStyle(LineStyle ls) +{ + mLineStyle = ls; +} + +/*! + Sets the visual appearance of single data points in the plot. If set to \ref QCPScatterStyle::ssNone, no scatter points + are drawn (e.g. for line-only-plots with appropriate line style). + + \see QCPScatterStyle, setLineStyle +*/ +void QCPGraph::setScatterStyle(const QCPScatterStyle &style) +{ + mScatterStyle = style; +} + +/*! + Sets which kind of error bars (Key Error, Value Error or both) should be drawn on each data + point. If you set \a errorType to something other than \ref etNone, make sure to actually pass + error data via the specific setData functions along with the data points (e.g. \ref + setDataValueError, \ref setDataKeyError, \ref setDataBothError). + + \see ErrorType +*/ +void QCPGraph::setErrorType(ErrorType errorType) +{ + mErrorType = errorType; +} + +/*! + Sets the pen with which the error bars will be drawn. + \see setErrorBarSize, setErrorType +*/ +void QCPGraph::setErrorPen(const QPen &pen) +{ + mErrorPen = pen; +} + +/*! + Sets the width of the handles at both ends of an error bar in pixels. +*/ +void QCPGraph::setErrorBarSize(double size) +{ + mErrorBarSize = size; +} + +/*! + If \a enabled is set to true, the error bar will not be drawn as a solid line under the scatter symbol but + leave some free space around the symbol. + + This feature uses the current scatter size (\ref QCPScatterStyle::setSize) to determine the size + of the area to leave blank. So when drawing Pixmaps as scatter points (\ref + QCPScatterStyle::ssPixmap), the scatter size must be set manually to a value corresponding to the + size of the Pixmap, if the error bars should leave gaps to its boundaries. + + \ref setErrorType, setErrorBarSize, setScatterStyle +*/ +void QCPGraph::setErrorBarSkipSymbol(bool enabled) +{ + mErrorBarSkipSymbol = enabled; +} + +/*! + Sets the target graph for filling the area between this graph and \a targetGraph with the current + brush (\ref setBrush). + + When \a targetGraph is set to 0, a normal graph fill to the zero-value-line will be shown. To + disable any filling, set the brush to Qt::NoBrush. + + \see setBrush +*/ +void QCPGraph::setChannelFillGraph(QCPGraph *targetGraph) +{ + // prevent setting channel target to this graph itself: + if (targetGraph == this) + { + qDebug() << Q_FUNC_INFO << "targetGraph is this graph itself"; + mChannelFillGraph = 0; + return; + } + // prevent setting channel target to a graph not in the plot: + if (targetGraph && targetGraph->mParentPlot != mParentPlot) + { + qDebug() << Q_FUNC_INFO << "targetGraph not in same plot"; + mChannelFillGraph = 0; + return; + } + + mChannelFillGraph = targetGraph; +} + +/*! + Sets whether adaptive sampling shall be used when plotting this graph. QCustomPlot's adaptive + sampling technique can drastically improve the replot performance for graphs with a larger number + of points (e.g. above 10,000), without notably changing the appearance of the graph. + + By default, adaptive sampling is enabled. Even if enabled, QCustomPlot decides whether adaptive + sampling shall actually be used on a per-graph basis. So leaving adaptive sampling enabled has no + disadvantage in almost all cases. + + \image html adaptive-sampling-line.png "A line plot of 500,000 points without and with adaptive sampling" + + As can be seen, line plots experience no visual degradation from adaptive sampling. Outliers are + reproduced reliably, as well as the overall shape of the data set. The replot time reduces + dramatically though. This allows QCustomPlot to display large amounts of data in realtime. + + \image html adaptive-sampling-scatter.png "A scatter plot of 100,000 points without and with adaptive sampling" + + Care must be taken when using high-density scatter plots in combination with adaptive sampling. + The adaptive sampling algorithm treats scatter plots more carefully than line plots which still + gives a significant reduction of replot times, but not quite as much as for line plots. This is + because scatter plots inherently need more data points to be preserved in order to still resemble + the original, non-adaptive-sampling plot. As shown above, the results still aren't quite + identical, as banding occurs for the outer data points. This is in fact intentional, such that + the boundaries of the data cloud stay visible to the viewer. How strong the banding appears, + depends on the point density, i.e. the number of points in the plot. + + For some situations with scatter plots it might thus be desirable to manually turn adaptive + sampling off. For example, when saving the plot to disk. This can be achieved by setting \a + enabled to false before issuing a command like \ref QCustomPlot::savePng, and setting \a enabled + back to true afterwards. +*/ +void QCPGraph::setAdaptiveSampling(bool enabled) +{ + mAdaptiveSampling = enabled; +} + +/*! + Adds the provided data points in \a dataMap to the current data. + + Alternatively, you can also access and modify the graph's data via the \ref data method, which + returns a pointer to the internal \ref QCPDataMap. + + \see removeData +*/ +void QCPGraph::addData(const QCPDataMap &dataMap) +{ + mData->unite(dataMap); +} + +/*! \overload + Adds the provided single data point in \a data to the current data. + + Alternatively, you can also access and modify the graph's data via the \ref data method, which + returns a pointer to the internal \ref QCPDataMap. + + \see removeData +*/ +void QCPGraph::addData(const QCPData &data) +{ + mData->insertMulti(data.key, data); +} + +/*! \overload + Adds the provided single data point as \a key and \a value pair to the current data. + + Alternatively, you can also access and modify the graph's data via the \ref data method, which + returns a pointer to the internal \ref QCPDataMap. + + \see removeData +*/ +void QCPGraph::addData(double key, double value) +{ + QCPData newData; + newData.key = key; + newData.value = value; + mData->insertMulti(newData.key, newData); +} + +/*! \overload + Adds the provided data points as \a key and \a value pairs to the current data. + + Alternatively, you can also access and modify the graph's data via the \ref data method, which + returns a pointer to the internal \ref QCPDataMap. + + \see removeData +*/ +void QCPGraph::addData(const QVector &keys, const QVector &values) +{ + int n = qMin(keys.size(), values.size()); + QCPData newData; + for (int i=0; iinsertMulti(newData.key, newData); + } +} + +/*! + Removes all data points with keys smaller than \a key. + \see addData, clearData +*/ +void QCPGraph::removeDataBefore(double key) +{ + QCPDataMap::iterator it = mData->begin(); + while (it != mData->end() && it.key() < key) + it = mData->erase(it); +} + +/*! + Removes all data points with keys greater than \a key. + \see addData, clearData +*/ +void QCPGraph::removeDataAfter(double key) +{ + if (mData->isEmpty()) return; + QCPDataMap::iterator it = mData->upperBound(key); + while (it != mData->end()) + it = mData->erase(it); +} + +/*! + Removes all data points with keys between \a fromKey and \a toKey. + if \a fromKey is greater or equal to \a toKey, the function does nothing. To remove + a single data point with known key, use \ref removeData(double key). + + \see addData, clearData +*/ +void QCPGraph::removeData(double fromKey, double toKey) +{ + if (fromKey >= toKey || mData->isEmpty()) return; + QCPDataMap::iterator it = mData->upperBound(fromKey); + QCPDataMap::iterator itEnd = mData->upperBound(toKey); + while (it != itEnd) + it = mData->erase(it); +} + +/*! \overload + + Removes a single data point at \a key. If the position is not known with absolute precision, + consider using \ref removeData(double fromKey, double toKey) with a small fuzziness interval around + the suspected position, depeding on the precision with which the key is known. + + \see addData, clearData +*/ +void QCPGraph::removeData(double key) +{ + mData->remove(key); +} + +/*! + Removes all data points. + \see removeData, removeDataAfter, removeDataBefore +*/ +void QCPGraph::clearData() +{ + mData->clear(); +} + +/* inherits documentation from base class */ +double QCPGraph::selectTest(const QPointF &pos, bool onlySelectable, QVariant *details) const +{ + Q_UNUSED(details) + if ((onlySelectable && !mSelectable) || mData->isEmpty()) + return -1; + if (!mKeyAxis || !mValueAxis) { qDebug() << Q_FUNC_INFO << "invalid key or value axis"; return -1; } + + if (mKeyAxis.data()->axisRect()->rect().contains(pos.toPoint())) + return pointDistance(pos); + else + return -1; +} + +/*! \overload + + Allows to define whether error bars are taken into consideration when determining the new axis + range. + + \see rescaleKeyAxis, rescaleValueAxis, QCPAbstractPlottable::rescaleAxes, QCustomPlot::rescaleAxes +*/ +void QCPGraph::rescaleAxes(bool onlyEnlarge, bool includeErrorBars) const +{ + rescaleKeyAxis(onlyEnlarge, includeErrorBars); + rescaleValueAxis(onlyEnlarge, includeErrorBars); +} + +/*! \overload + + Allows to define whether error bars (of kind \ref QCPGraph::etKey) are taken into consideration + when determining the new axis range. + + \see rescaleAxes, QCPAbstractPlottable::rescaleKeyAxis +*/ +void QCPGraph::rescaleKeyAxis(bool onlyEnlarge, bool includeErrorBars) const +{ + // this code is a copy of QCPAbstractPlottable::rescaleKeyAxis with the only change + // that getKeyRange is passed the includeErrorBars value. + if (mData->isEmpty()) return; + + QCPAxis *keyAxis = mKeyAxis.data(); + if (!keyAxis) { qDebug() << Q_FUNC_INFO << "invalid key axis"; return; } + + SignDomain signDomain = sdBoth; + if (keyAxis->scaleType() == QCPAxis::stLogarithmic) + signDomain = (keyAxis->range().upper < 0 ? sdNegative : sdPositive); + + bool foundRange; + QCPRange newRange = getKeyRange(foundRange, signDomain, includeErrorBars); + + if (foundRange) + { + if (onlyEnlarge) + { + if (keyAxis->range().lower < newRange.lower) + newRange.lower = keyAxis->range().lower; + if (keyAxis->range().upper > newRange.upper) + newRange.upper = keyAxis->range().upper; + } + keyAxis->setRange(newRange); + } +} + +/*! \overload + + Allows to define whether error bars (of kind \ref QCPGraph::etValue) are taken into consideration + when determining the new axis range. + + \see rescaleAxes, QCPAbstractPlottable::rescaleValueAxis +*/ +void QCPGraph::rescaleValueAxis(bool onlyEnlarge, bool includeErrorBars) const +{ + // this code is a copy of QCPAbstractPlottable::rescaleValueAxis with the only change + // is that getValueRange is passed the includeErrorBars value. + if (mData->isEmpty()) return; + + QCPAxis *valueAxis = mValueAxis.data(); + if (!valueAxis) { qDebug() << Q_FUNC_INFO << "invalid value axis"; return; } + + SignDomain signDomain = sdBoth; + if (valueAxis->scaleType() == QCPAxis::stLogarithmic) + signDomain = (valueAxis->range().upper < 0 ? sdNegative : sdPositive); + + bool foundRange; + QCPRange newRange = getValueRange(foundRange, signDomain, includeErrorBars); + + if (foundRange) + { + if (onlyEnlarge) + { + if (valueAxis->range().lower < newRange.lower) + newRange.lower = valueAxis->range().lower; + if (valueAxis->range().upper > newRange.upper) + newRange.upper = valueAxis->range().upper; + } + valueAxis->setRange(newRange); + } +} + +/* inherits documentation from base class */ +void QCPGraph::draw(QCPPainter *painter) +{ + if (!mKeyAxis || !mValueAxis) { qDebug() << Q_FUNC_INFO << "invalid key or value axis"; return; } + if (mKeyAxis.data()->range().size() <= 0 || mData->isEmpty()) return; + if (mLineStyle == lsNone && mScatterStyle.isNone()) return; + + // allocate line and (if necessary) point vectors: + QVector *lineData = new QVector; + QVector *scatterData = 0; + if (!mScatterStyle.isNone()) + scatterData = new QVector; + + // fill vectors with data appropriate to plot style: + getPlotData(lineData, scatterData); + + // check data validity if flag set: +#ifdef QCUSTOMPLOT_CHECK_DATA + QCPDataMap::const_iterator it; + for (it = mData->constBegin(); it != mData->constEnd(); ++it) + { + if (QCP::isInvalidData(it.value().key, it.value().value) || + QCP::isInvalidData(it.value().keyErrorPlus, it.value().keyErrorMinus) || + QCP::isInvalidData(it.value().valueErrorPlus, it.value().valueErrorPlus)) + qDebug() << Q_FUNC_INFO << "Data point at" << it.key() << "invalid." << "Plottable name:" << name(); + } +#endif + + // draw fill of graph: + if (mLineStyle != lsNone) + drawFill(painter, lineData); + + // draw line: + if (mLineStyle == lsImpulse) + drawImpulsePlot(painter, lineData); + else if (mLineStyle != lsNone) + drawLinePlot(painter, lineData); // also step plots can be drawn as a line plot + + // draw scatters: + if (scatterData) + drawScatterPlot(painter, scatterData); + + // free allocated line and point vectors: + delete lineData; + if (scatterData) + delete scatterData; +} + +/* inherits documentation from base class */ +void QCPGraph::drawLegendIcon(QCPPainter *painter, const QRectF &rect) const +{ + // draw fill: + if (mBrush.style() != Qt::NoBrush) + { + applyFillAntialiasingHint(painter); + painter->fillRect(QRectF(rect.left(), rect.top()+rect.height()/2.0, rect.width(), rect.height()/3.0), mBrush); + } + // draw line vertically centered: + if (mLineStyle != lsNone) + { + applyDefaultAntialiasingHint(painter); + painter->setPen(mPen); + painter->drawLine(QLineF(rect.left(), rect.top()+rect.height()/2.0, rect.right()+5, rect.top()+rect.height()/2.0)); // +5 on x2 else last segment is missing from dashed/dotted pens + } + // draw scatter symbol: + if (!mScatterStyle.isNone()) + { + applyScattersAntialiasingHint(painter); + // scale scatter pixmap if it's too large to fit in legend icon rect: + if (mScatterStyle.shape() == QCPScatterStyle::ssPixmap && (mScatterStyle.pixmap().size().width() > rect.width() || mScatterStyle.pixmap().size().height() > rect.height())) + { + QCPScatterStyle scaledStyle(mScatterStyle); + scaledStyle.setPixmap(scaledStyle.pixmap().scaled(rect.size().toSize(), Qt::KeepAspectRatio, Qt::SmoothTransformation)); + scaledStyle.applyTo(painter, mPen); + scaledStyle.drawShape(painter, QRectF(rect).center()); + } else + { + mScatterStyle.applyTo(painter, mPen); + mScatterStyle.drawShape(painter, QRectF(rect).center()); + } + } +} + +/*! \internal + + This function branches out to the line style specific "get(...)PlotData" functions, according to + the line style of the graph. + + \a lineData will be filled with raw points that will be drawn with the according draw functions, + e.g. \ref drawLinePlot and \ref drawImpulsePlot. These aren't necessarily the original data + points, since for step plots for example, additional points are needed for drawing lines that + make up steps. If the line style of the graph is \ref lsNone, the \a lineData vector will be left + untouched. + + \a scatterData will be filled with the original data points so \ref drawScatterPlot can draw the + scatter symbols accordingly. If no scatters need to be drawn, i.e. the scatter style's shape is + \ref QCPScatterStyle::ssNone, pass 0 as \a scatterData, and this step will be skipped. + + \see getScatterPlotData, getLinePlotData, getStepLeftPlotData, getStepRightPlotData, + getStepCenterPlotData, getImpulsePlotData +*/ +void QCPGraph::getPlotData(QVector *lineData, QVector *scatterData) const +{ + switch(mLineStyle) + { + case lsNone: getScatterPlotData(scatterData); break; + case lsLine: getLinePlotData(lineData, scatterData); break; + case lsStepLeft: getStepLeftPlotData(lineData, scatterData); break; + case lsStepRight: getStepRightPlotData(lineData, scatterData); break; + case lsStepCenter: getStepCenterPlotData(lineData, scatterData); break; + case lsImpulse: getImpulsePlotData(lineData, scatterData); break; + } +} + +/*! \internal + + If line style is \ref lsNone and the scatter style's shape is not \ref QCPScatterStyle::ssNone, + this function serves at providing the visible data points in \a scatterData, so the \ref + drawScatterPlot function can draw the scatter points accordingly. + + If line style is not \ref lsNone, this function is not called and the data for the scatter points + are (if needed) calculated inside the corresponding other "get(...)PlotData" functions. + + \see drawScatterPlot +*/ +void QCPGraph::getScatterPlotData(QVector *scatterData) const +{ + getPreparedData(0, scatterData); +} + +/*! \internal + + Places the raw data points needed for a normal linearly connected graph in \a linePixelData. + + As for all plot data retrieval functions, \a scatterData just contains all unaltered data (scatter) + points that are visible for drawing scatter points, if necessary. If drawing scatter points is + disabled (i.e. the scatter style's shape is \ref QCPScatterStyle::ssNone), pass 0 as \a + scatterData, and the function will skip filling the vector. + + \see drawLinePlot +*/ +void QCPGraph::getLinePlotData(QVector *linePixelData, QVector *scatterData) const +{ + QCPAxis *keyAxis = mKeyAxis.data(); + QCPAxis *valueAxis = mValueAxis.data(); + if (!keyAxis || !valueAxis) { qDebug() << Q_FUNC_INFO << "invalid key or value axis"; return; } + if (!linePixelData) { qDebug() << Q_FUNC_INFO << "null pointer passed as linePixelData"; return; } + + QVector lineData; + getPreparedData(&lineData, scatterData); + linePixelData->reserve(lineData.size()+2); // added 2 to reserve memory for lower/upper fill base points that might be needed for fill + linePixelData->resize(lineData.size()); + + // transform lineData points to pixels: + if (keyAxis->orientation() == Qt::Vertical) + { + for (int i=0; icoordToPixel(lineData.at(i).value)); + (*linePixelData)[i].setY(keyAxis->coordToPixel(lineData.at(i).key)); + } + } else // key axis is horizontal + { + for (int i=0; icoordToPixel(lineData.at(i).key)); + (*linePixelData)[i].setY(valueAxis->coordToPixel(lineData.at(i).value)); + } + } +} + +/*! + \internal + Places the raw data points needed for a step plot with left oriented steps in \a lineData. + + As for all plot data retrieval functions, \a scatterData just contains all unaltered data (scatter) + points that are visible for drawing scatter points, if necessary. If drawing scatter points is + disabled (i.e. the scatter style's shape is \ref QCPScatterStyle::ssNone), pass 0 as \a + scatterData, and the function will skip filling the vector. + + \see drawLinePlot +*/ +void QCPGraph::getStepLeftPlotData(QVector *linePixelData, QVector *scatterData) const +{ + QCPAxis *keyAxis = mKeyAxis.data(); + QCPAxis *valueAxis = mValueAxis.data(); + if (!keyAxis || !valueAxis) { qDebug() << Q_FUNC_INFO << "invalid key or value axis"; return; } + if (!linePixelData) { qDebug() << Q_FUNC_INFO << "null pointer passed as lineData"; return; } + + QVector lineData; + getPreparedData(&lineData, scatterData); + linePixelData->reserve(lineData.size()*2+2); // added 2 to reserve memory for lower/upper fill base points that might be needed for fill + linePixelData->resize(lineData.size()*2); + + // calculate steps from lineData and transform to pixel coordinates: + if (keyAxis->orientation() == Qt::Vertical) + { + double lastValue = valueAxis->coordToPixel(lineData.first().value); + double key; + for (int i=0; icoordToPixel(lineData.at(i).key); + (*linePixelData)[i*2+0].setX(lastValue); + (*linePixelData)[i*2+0].setY(key); + lastValue = valueAxis->coordToPixel(lineData.at(i).value); + (*linePixelData)[i*2+1].setX(lastValue); + (*linePixelData)[i*2+1].setY(key); + } + } else // key axis is horizontal + { + double lastValue = valueAxis->coordToPixel(lineData.first().value); + double key; + for (int i=0; icoordToPixel(lineData.at(i).key); + (*linePixelData)[i*2+0].setX(key); + (*linePixelData)[i*2+0].setY(lastValue); + lastValue = valueAxis->coordToPixel(lineData.at(i).value); + (*linePixelData)[i*2+1].setX(key); + (*linePixelData)[i*2+1].setY(lastValue); + } + } +} + +/*! + \internal + Places the raw data points needed for a step plot with right oriented steps in \a lineData. + + As for all plot data retrieval functions, \a scatterData just contains all unaltered data (scatter) + points that are visible for drawing scatter points, if necessary. If drawing scatter points is + disabled (i.e. the scatter style's shape is \ref QCPScatterStyle::ssNone), pass 0 as \a + scatterData, and the function will skip filling the vector. + + \see drawLinePlot +*/ +void QCPGraph::getStepRightPlotData(QVector *linePixelData, QVector *scatterData) const +{ + QCPAxis *keyAxis = mKeyAxis.data(); + QCPAxis *valueAxis = mValueAxis.data(); + if (!keyAxis || !valueAxis) { qDebug() << Q_FUNC_INFO << "invalid key or value axis"; return; } + if (!linePixelData) { qDebug() << Q_FUNC_INFO << "null pointer passed as lineData"; return; } + + QVector lineData; + getPreparedData(&lineData, scatterData); + linePixelData->reserve(lineData.size()*2+2); // added 2 to reserve memory for lower/upper fill base points that might be needed for fill + linePixelData->resize(lineData.size()*2); + + // calculate steps from lineData and transform to pixel coordinates: + if (keyAxis->orientation() == Qt::Vertical) + { + double lastKey = keyAxis->coordToPixel(lineData.first().key); + double value; + for (int i=0; icoordToPixel(lineData.at(i).value); + (*linePixelData)[i*2+0].setX(value); + (*linePixelData)[i*2+0].setY(lastKey); + lastKey = keyAxis->coordToPixel(lineData.at(i).key); + (*linePixelData)[i*2+1].setX(value); + (*linePixelData)[i*2+1].setY(lastKey); + } + } else // key axis is horizontal + { + double lastKey = keyAxis->coordToPixel(lineData.first().key); + double value; + for (int i=0; icoordToPixel(lineData.at(i).value); + (*linePixelData)[i*2+0].setX(lastKey); + (*linePixelData)[i*2+0].setY(value); + lastKey = keyAxis->coordToPixel(lineData.at(i).key); + (*linePixelData)[i*2+1].setX(lastKey); + (*linePixelData)[i*2+1].setY(value); + } + } +} + +/*! + \internal + Places the raw data points needed for a step plot with centered steps in \a lineData. + + As for all plot data retrieval functions, \a scatterData just contains all unaltered data (scatter) + points that are visible for drawing scatter points, if necessary. If drawing scatter points is + disabled (i.e. the scatter style's shape is \ref QCPScatterStyle::ssNone), pass 0 as \a + scatterData, and the function will skip filling the vector. + + \see drawLinePlot +*/ +void QCPGraph::getStepCenterPlotData(QVector *linePixelData, QVector *scatterData) const +{ + QCPAxis *keyAxis = mKeyAxis.data(); + QCPAxis *valueAxis = mValueAxis.data(); + if (!keyAxis || !valueAxis) { qDebug() << Q_FUNC_INFO << "invalid key or value axis"; return; } + if (!linePixelData) { qDebug() << Q_FUNC_INFO << "null pointer passed as lineData"; return; } + + QVector lineData; + getPreparedData(&lineData, scatterData); + linePixelData->reserve(lineData.size()*2+2); // added 2 to reserve memory for lower/upper fill base points that might be needed for fill + linePixelData->resize(lineData.size()*2); + // calculate steps from lineData and transform to pixel coordinates: + if (keyAxis->orientation() == Qt::Vertical) + { + double lastKey = keyAxis->coordToPixel(lineData.first().key); + double lastValue = valueAxis->coordToPixel(lineData.first().value); + double key; + (*linePixelData)[0].setX(lastValue); + (*linePixelData)[0].setY(lastKey); + for (int i=1; icoordToPixel(lineData.at(i).key)+lastKey)*0.5; + (*linePixelData)[i*2-1].setX(lastValue); + (*linePixelData)[i*2-1].setY(key); + lastValue = valueAxis->coordToPixel(lineData.at(i).value); + lastKey = keyAxis->coordToPixel(lineData.at(i).key); + (*linePixelData)[i*2+0].setX(lastValue); + (*linePixelData)[i*2+0].setY(key); + } + (*linePixelData)[lineData.size()*2-1].setX(lastValue); + (*linePixelData)[lineData.size()*2-1].setY(lastKey); + } else // key axis is horizontal + { + double lastKey = keyAxis->coordToPixel(lineData.first().key); + double lastValue = valueAxis->coordToPixel(lineData.first().value); + double key; + (*linePixelData)[0].setX(lastKey); + (*linePixelData)[0].setY(lastValue); + for (int i=1; icoordToPixel(lineData.at(i).key)+lastKey)*0.5; + (*linePixelData)[i*2-1].setX(key); + (*linePixelData)[i*2-1].setY(lastValue); + lastValue = valueAxis->coordToPixel(lineData.at(i).value); + lastKey = keyAxis->coordToPixel(lineData.at(i).key); + (*linePixelData)[i*2+0].setX(key); + (*linePixelData)[i*2+0].setY(lastValue); + } + (*linePixelData)[lineData.size()*2-1].setX(lastKey); + (*linePixelData)[lineData.size()*2-1].setY(lastValue); + } + +} + +/*! + \internal + Places the raw data points needed for an impulse plot in \a lineData. + + As for all plot data retrieval functions, \a scatterData just contains all unaltered data (scatter) + points that are visible for drawing scatter points, if necessary. If drawing scatter points is + disabled (i.e. the scatter style's shape is \ref QCPScatterStyle::ssNone), pass 0 as \a + scatterData, and the function will skip filling the vector. + + \see drawImpulsePlot +*/ +void QCPGraph::getImpulsePlotData(QVector *linePixelData, QVector *scatterData) const +{ + QCPAxis *keyAxis = mKeyAxis.data(); + QCPAxis *valueAxis = mValueAxis.data(); + if (!keyAxis || !valueAxis) { qDebug() << Q_FUNC_INFO << "invalid key or value axis"; return; } + if (!linePixelData) { qDebug() << Q_FUNC_INFO << "null pointer passed as linePixelData"; return; } + + QVector lineData; + getPreparedData(&lineData, scatterData); + linePixelData->resize(lineData.size()*2); // no need to reserve 2 extra points because impulse plot has no fill + + // transform lineData points to pixels: + if (keyAxis->orientation() == Qt::Vertical) + { + double zeroPointX = valueAxis->coordToPixel(0); + double key; + for (int i=0; icoordToPixel(lineData.at(i).key); + (*linePixelData)[i*2+0].setX(zeroPointX); + (*linePixelData)[i*2+0].setY(key); + (*linePixelData)[i*2+1].setX(valueAxis->coordToPixel(lineData.at(i).value)); + (*linePixelData)[i*2+1].setY(key); + } + } else // key axis is horizontal + { + double zeroPointY = valueAxis->coordToPixel(0); + double key; + for (int i=0; icoordToPixel(lineData.at(i).key); + (*linePixelData)[i*2+0].setX(key); + (*linePixelData)[i*2+0].setY(zeroPointY); + (*linePixelData)[i*2+1].setX(key); + (*linePixelData)[i*2+1].setY(valueAxis->coordToPixel(lineData.at(i).value)); + } + } +} + +/*! \internal + + Draws the fill of the graph with the specified brush. + + If the fill is a normal fill towards the zero-value-line, only the \a lineData is required (and + two extra points at the zero-value-line, which are added by \ref addFillBasePoints and removed by + \ref removeFillBasePoints after the fill drawing is done). + + If the fill is a channel fill between this QCPGraph and another QCPGraph (mChannelFillGraph), the + more complex polygon is calculated with the \ref getChannelFillPolygon function. + + \see drawLinePlot +*/ +void QCPGraph::drawFill(QCPPainter *painter, QVector *lineData) const +{ + if (mLineStyle == lsImpulse) return; // fill doesn't make sense for impulse plot + if (mainBrush().style() == Qt::NoBrush || mainBrush().color().alpha() == 0) return; + + applyFillAntialiasingHint(painter); + if (!mChannelFillGraph) + { + // draw base fill under graph, fill goes all the way to the zero-value-line: + addFillBasePoints(lineData); + painter->setPen(Qt::NoPen); + painter->setBrush(mainBrush()); + painter->drawPolygon(QPolygonF(*lineData)); + removeFillBasePoints(lineData); + } else + { + // draw channel fill between this graph and mChannelFillGraph: + painter->setPen(Qt::NoPen); + painter->setBrush(mainBrush()); + painter->drawPolygon(getChannelFillPolygon(lineData)); + } +} + +/*! \internal + + Draws scatter symbols at every data point passed in \a scatterData. scatter symbols are independent + of the line style and are always drawn if the scatter style's shape is not \ref + QCPScatterStyle::ssNone. Hence, the \a scatterData vector is outputted by all "get(...)PlotData" + functions, together with the (line style dependent) line data. + + \see drawLinePlot, drawImpulsePlot +*/ +void QCPGraph::drawScatterPlot(QCPPainter *painter, QVector *scatterData) const +{ + QCPAxis *keyAxis = mKeyAxis.data(); + QCPAxis *valueAxis = mValueAxis.data(); + if (!keyAxis || !valueAxis) { qDebug() << Q_FUNC_INFO << "invalid key or value axis"; return; } + + // draw error bars: + if (mErrorType != etNone) + { + applyErrorBarsAntialiasingHint(painter); + painter->setPen(mErrorPen); + if (keyAxis->orientation() == Qt::Vertical) + { + for (int i=0; isize(); ++i) + drawError(painter, valueAxis->coordToPixel(scatterData->at(i).value), keyAxis->coordToPixel(scatterData->at(i).key), scatterData->at(i)); + } else + { + for (int i=0; isize(); ++i) + drawError(painter, keyAxis->coordToPixel(scatterData->at(i).key), valueAxis->coordToPixel(scatterData->at(i).value), scatterData->at(i)); + } + } + + // draw scatter point symbols: + applyScattersAntialiasingHint(painter); + mScatterStyle.applyTo(painter, mPen); + if (keyAxis->orientation() == Qt::Vertical) + { + for (int i=0; isize(); ++i) + if (!qIsNaN(scatterData->at(i).value)) + mScatterStyle.drawShape(painter, valueAxis->coordToPixel(scatterData->at(i).value), keyAxis->coordToPixel(scatterData->at(i).key)); + } else + { + for (int i=0; isize(); ++i) + if (!qIsNaN(scatterData->at(i).value)) + mScatterStyle.drawShape(painter, keyAxis->coordToPixel(scatterData->at(i).key), valueAxis->coordToPixel(scatterData->at(i).value)); + } +} + +/*! \internal + + Draws line graphs from the provided data. It connects all points in \a lineData, which was + created by one of the "get(...)PlotData" functions for line styles that require simple line + connections between the point vector they create. These are for example \ref getLinePlotData, + \ref getStepLeftPlotData, \ref getStepRightPlotData and \ref getStepCenterPlotData. + + \see drawScatterPlot, drawImpulsePlot +*/ +void QCPGraph::drawLinePlot(QCPPainter *painter, QVector *lineData) const +{ + // draw line of graph: + if (mainPen().style() != Qt::NoPen && mainPen().color().alpha() != 0) + { + applyDefaultAntialiasingHint(painter); + painter->setPen(mainPen()); + painter->setBrush(Qt::NoBrush); + + /* Draws polyline in batches, currently not used: + int p = 0; + while (p < lineData->size()) + { + int batch = qMin(25, lineData->size()-p); + if (p != 0) + { + ++batch; + --p; // to draw the connection lines between two batches + } + painter->drawPolyline(lineData->constData()+p, batch); + p += batch; + } + */ + + // if drawing solid line and not in PDF, use much faster line drawing instead of polyline: + if (mParentPlot->plottingHints().testFlag(QCP::phFastPolylines) && + painter->pen().style() == Qt::SolidLine && + !painter->modes().testFlag(QCPPainter::pmVectorized) && + !painter->modes().testFlag(QCPPainter::pmNoCaching)) + { + int i = 0; + bool lastIsNan = false; + const int lineDataSize = lineData->size(); + while (i < lineDataSize && (qIsNaN(lineData->at(i).y()) || qIsNaN(lineData->at(i).x()))) // make sure first point is not NaN + ++i; + ++i; // because drawing works in 1 point retrospect + while (i < lineDataSize) + { + if (!qIsNaN(lineData->at(i).y()) && !qIsNaN(lineData->at(i).x())) // NaNs create a gap in the line + { + if (!lastIsNan) + painter->drawLine(lineData->at(i-1), lineData->at(i)); + else + lastIsNan = false; + } else + lastIsNan = true; + ++i; + } + } else + { + int segmentStart = 0; + int i = 0; + const int lineDataSize = lineData->size(); + while (i < lineDataSize) + { + if (qIsNaN(lineData->at(i).y()) || qIsNaN(lineData->at(i).x()) || qIsInf(lineData->at(i).y())) // NaNs create a gap in the line. Also filter Infs which make drawPolyline block + { + painter->drawPolyline(lineData->constData()+segmentStart, i-segmentStart); // i, because we don't want to include the current NaN point + segmentStart = i+1; + } + ++i; + } + // draw last segment: + painter->drawPolyline(lineData->constData()+segmentStart, lineDataSize-segmentStart); + } + } +} + +/*! \internal + + Draws impulses from the provided data, i.e. it connects all line pairs in \a lineData, which was + created by \ref getImpulsePlotData. + + \see drawScatterPlot, drawLinePlot +*/ +void QCPGraph::drawImpulsePlot(QCPPainter *painter, QVector *lineData) const +{ + // draw impulses: + if (mainPen().style() != Qt::NoPen && mainPen().color().alpha() != 0) + { + applyDefaultAntialiasingHint(painter); + QPen pen = mainPen(); + pen.setCapStyle(Qt::FlatCap); // so impulse line doesn't reach beyond zero-line + painter->setPen(pen); + painter->setBrush(Qt::NoBrush); + painter->drawLines(*lineData); + } +} + +/*! \internal + + Returns the \a lineData and \a scatterData that need to be plotted for this graph taking into + consideration the current axis ranges and, if \ref setAdaptiveSampling is enabled, local point + densities. + + 0 may be passed as \a lineData or \a scatterData to indicate that the respective dataset isn't + needed. For example, if the scatter style (\ref setScatterStyle) is \ref QCPScatterStyle::ssNone, \a + scatterData should be 0 to prevent unnecessary calculations. + + This method is used by the various "get(...)PlotData" methods to get the basic working set of data. +*/ +void QCPGraph::getPreparedData(QVector *lineData, QVector *scatterData) const +{ + QCPAxis *keyAxis = mKeyAxis.data(); + QCPAxis *valueAxis = mValueAxis.data(); + if (!keyAxis || !valueAxis) { qDebug() << Q_FUNC_INFO << "invalid key or value axis"; return; } + // get visible data range: + QCPDataMap::const_iterator lower, upper; // note that upper is the actual upper point, and not 1 step after the upper point + getVisibleDataBounds(lower, upper); + if (lower == mData->constEnd() || upper == mData->constEnd()) + return; + + // count points in visible range, taking into account that we only need to count to the limit maxCount if using adaptive sampling: + int maxCount = std::numeric_limits::max(); + if (mAdaptiveSampling) + { + int keyPixelSpan = qAbs(keyAxis->coordToPixel(lower.key())-keyAxis->coordToPixel(upper.key())); + maxCount = 2*keyPixelSpan+2; + } + int dataCount = countDataInBounds(lower, upper, maxCount); + + if (mAdaptiveSampling && dataCount >= maxCount) // use adaptive sampling only if there are at least two points per pixel on average + { + if (lineData) + { + QCPDataMap::const_iterator it = lower; + QCPDataMap::const_iterator upperEnd = upper+1; + double minValue = it.value().value; + double maxValue = it.value().value; + QCPDataMap::const_iterator currentIntervalFirstPoint = it; + int reversedFactor = keyAxis->rangeReversed() != (keyAxis->orientation()==Qt::Vertical) ? -1 : 1; // is used to calculate keyEpsilon pixel into the correct direction + int reversedRound = keyAxis->rangeReversed() != (keyAxis->orientation()==Qt::Vertical) ? 1 : 0; // is used to switch between floor (normal) and ceil (reversed) rounding of currentIntervalStartKey + double currentIntervalStartKey = keyAxis->pixelToCoord((int)(keyAxis->coordToPixel(lower.key())+reversedRound)); + double lastIntervalEndKey = currentIntervalStartKey; + double keyEpsilon = qAbs(currentIntervalStartKey-keyAxis->pixelToCoord(keyAxis->coordToPixel(currentIntervalStartKey)+1.0*reversedFactor)); // interval of one pixel on screen when mapped to plot key coordinates + bool keyEpsilonVariable = keyAxis->scaleType() == QCPAxis::stLogarithmic; // indicates whether keyEpsilon needs to be updated after every interval (for log axes) + int intervalDataCount = 1; + ++it; // advance iterator to second data point because adaptive sampling works in 1 point retrospect + while (it != upperEnd) + { + if (it.key() < currentIntervalStartKey+keyEpsilon) // data point is still within same pixel, so skip it and expand value span of this cluster if necessary + { + if (it.value().value < minValue) + minValue = it.value().value; + else if (it.value().value > maxValue) + maxValue = it.value().value; + ++intervalDataCount; + } else // new pixel interval started + { + if (intervalDataCount >= 2) // last pixel had multiple data points, consolidate them to a cluster + { + if (lastIntervalEndKey < currentIntervalStartKey-keyEpsilon) // last point is further away, so first point of this cluster must be at a real data point + lineData->append(QCPData(currentIntervalStartKey+keyEpsilon*0.2, currentIntervalFirstPoint.value().value)); + lineData->append(QCPData(currentIntervalStartKey+keyEpsilon*0.25, minValue)); + lineData->append(QCPData(currentIntervalStartKey+keyEpsilon*0.75, maxValue)); + if (it.key() > currentIntervalStartKey+keyEpsilon*2) // new pixel started further away from previous cluster, so make sure the last point of the cluster is at a real data point + lineData->append(QCPData(currentIntervalStartKey+keyEpsilon*0.8, (it-1).value().value)); + } else + lineData->append(QCPData(currentIntervalFirstPoint.key(), currentIntervalFirstPoint.value().value)); + lastIntervalEndKey = (it-1).value().key; + minValue = it.value().value; + maxValue = it.value().value; + currentIntervalFirstPoint = it; + currentIntervalStartKey = keyAxis->pixelToCoord((int)(keyAxis->coordToPixel(it.key())+reversedRound)); + if (keyEpsilonVariable) + keyEpsilon = qAbs(currentIntervalStartKey-keyAxis->pixelToCoord(keyAxis->coordToPixel(currentIntervalStartKey)+1.0*reversedFactor)); + intervalDataCount = 1; + } + ++it; + } + // handle last interval: + if (intervalDataCount >= 2) // last pixel had multiple data points, consolidate them to a cluster + { + if (lastIntervalEndKey < currentIntervalStartKey-keyEpsilon) // last point wasn't a cluster, so first point of this cluster must be at a real data point + lineData->append(QCPData(currentIntervalStartKey+keyEpsilon*0.2, currentIntervalFirstPoint.value().value)); + lineData->append(QCPData(currentIntervalStartKey+keyEpsilon*0.25, minValue)); + lineData->append(QCPData(currentIntervalStartKey+keyEpsilon*0.75, maxValue)); + } else + lineData->append(QCPData(currentIntervalFirstPoint.key(), currentIntervalFirstPoint.value().value)); + } + + if (scatterData) + { + double valueMaxRange = valueAxis->range().upper; + double valueMinRange = valueAxis->range().lower; + QCPDataMap::const_iterator it = lower; + QCPDataMap::const_iterator upperEnd = upper+1; + double minValue = it.value().value; + double maxValue = it.value().value; + QCPDataMap::const_iterator minValueIt = it; + QCPDataMap::const_iterator maxValueIt = it; + QCPDataMap::const_iterator currentIntervalStart = it; + int reversedFactor = keyAxis->rangeReversed() ? -1 : 1; // is used to calculate keyEpsilon pixel into the correct direction + int reversedRound = keyAxis->rangeReversed() ? 1 : 0; // is used to switch between floor (normal) and ceil (reversed) rounding of currentIntervalStartKey + double currentIntervalStartKey = keyAxis->pixelToCoord((int)(keyAxis->coordToPixel(lower.key())+reversedRound)); + double keyEpsilon = qAbs(currentIntervalStartKey-keyAxis->pixelToCoord(keyAxis->coordToPixel(currentIntervalStartKey)+1.0*reversedFactor)); // interval of one pixel on screen when mapped to plot key coordinates + bool keyEpsilonVariable = keyAxis->scaleType() == QCPAxis::stLogarithmic; // indicates whether keyEpsilon needs to be updated after every interval (for log axes) + int intervalDataCount = 1; + ++it; // advance iterator to second data point because adaptive sampling works in 1 point retrospect + while (it != upperEnd) + { + if (it.key() < currentIntervalStartKey+keyEpsilon) // data point is still within same pixel, so skip it and expand value span of this pixel if necessary + { + if (it.value().value < minValue && it.value().value > valueMinRange && it.value().value < valueMaxRange) + { + minValue = it.value().value; + minValueIt = it; + } else if (it.value().value > maxValue && it.value().value > valueMinRange && it.value().value < valueMaxRange) + { + maxValue = it.value().value; + maxValueIt = it; + } + ++intervalDataCount; + } else // new pixel started + { + if (intervalDataCount >= 2) // last pixel had multiple data points, consolidate them + { + // determine value pixel span and add as many points in interval to maintain certain vertical data density (this is specific to scatter plot): + double valuePixelSpan = qAbs(valueAxis->coordToPixel(minValue)-valueAxis->coordToPixel(maxValue)); + int dataModulo = qMax(1, qRound(intervalDataCount/(valuePixelSpan/4.0))); // approximately every 4 value pixels one data point on average + QCPDataMap::const_iterator intervalIt = currentIntervalStart; + int c = 0; + while (intervalIt != it) + { + if ((c % dataModulo == 0 || intervalIt == minValueIt || intervalIt == maxValueIt) && intervalIt.value().value > valueMinRange && intervalIt.value().value < valueMaxRange) + scatterData->append(intervalIt.value()); + ++c; + ++intervalIt; + } + } else if (currentIntervalStart.value().value > valueMinRange && currentIntervalStart.value().value < valueMaxRange) + scatterData->append(currentIntervalStart.value()); + minValue = it.value().value; + maxValue = it.value().value; + currentIntervalStart = it; + currentIntervalStartKey = keyAxis->pixelToCoord((int)(keyAxis->coordToPixel(it.key())+reversedRound)); + if (keyEpsilonVariable) + keyEpsilon = qAbs(currentIntervalStartKey-keyAxis->pixelToCoord(keyAxis->coordToPixel(currentIntervalStartKey)+1.0*reversedFactor)); + intervalDataCount = 1; + } + ++it; + } + // handle last interval: + if (intervalDataCount >= 2) // last pixel had multiple data points, consolidate them + { + // determine value pixel span and add as many points in interval to maintain certain vertical data density (this is specific to scatter plot): + double valuePixelSpan = qAbs(valueAxis->coordToPixel(minValue)-valueAxis->coordToPixel(maxValue)); + int dataModulo = qMax(1, qRound(intervalDataCount/(valuePixelSpan/4.0))); // approximately every 4 value pixels one data point on average + QCPDataMap::const_iterator intervalIt = currentIntervalStart; + int c = 0; + while (intervalIt != it) + { + if ((c % dataModulo == 0 || intervalIt == minValueIt || intervalIt == maxValueIt) && intervalIt.value().value > valueMinRange && intervalIt.value().value < valueMaxRange) + scatterData->append(intervalIt.value()); + ++c; + ++intervalIt; + } + } else if (currentIntervalStart.value().value > valueMinRange && currentIntervalStart.value().value < valueMaxRange) + scatterData->append(currentIntervalStart.value()); + } + } else // don't use adaptive sampling algorithm, transfer points one-to-one from the map into the output parameters + { + QVector *dataVector = 0; + if (lineData) + dataVector = lineData; + else if (scatterData) + dataVector = scatterData; + if (dataVector) + { + QCPDataMap::const_iterator it = lower; + QCPDataMap::const_iterator upperEnd = upper+1; + dataVector->reserve(dataCount+2); // +2 for possible fill end points + while (it != upperEnd) + { + dataVector->append(it.value()); + ++it; + } + } + if (lineData && scatterData) + *scatterData = *dataVector; + } +} + +/*! \internal + + called by the scatter drawing function (\ref drawScatterPlot) to draw the error bars on one data + point. \a x and \a y pixel positions of the data point are passed since they are already known in + pixel coordinates in the drawing function, so we save some extra coordToPixel transforms here. \a + data is therefore only used for the errors, not key and value. +*/ +void QCPGraph::drawError(QCPPainter *painter, double x, double y, const QCPData &data) const +{ + if (qIsNaN(data.value)) + return; + QCPAxis *keyAxis = mKeyAxis.data(); + QCPAxis *valueAxis = mValueAxis.data(); + if (!keyAxis || !valueAxis) { qDebug() << Q_FUNC_INFO << "invalid key or value axis"; return; } + + double a, b; // positions of error bar bounds in pixels + double barWidthHalf = mErrorBarSize*0.5; + double skipSymbolMargin = mScatterStyle.size(); // pixels left blank per side, when mErrorBarSkipSymbol is true + + if (keyAxis->orientation() == Qt::Vertical) + { + // draw key error vertically and value error horizontally + if (mErrorType == etKey || mErrorType == etBoth) + { + a = keyAxis->coordToPixel(data.key-data.keyErrorMinus); + b = keyAxis->coordToPixel(data.key+data.keyErrorPlus); + if (keyAxis->rangeReversed()) + qSwap(a,b); + // draw spine: + if (mErrorBarSkipSymbol) + { + if (a-y > skipSymbolMargin) // don't draw spine if error is so small it's within skipSymbolmargin + painter->drawLine(QLineF(x, a, x, y+skipSymbolMargin)); + if (y-b > skipSymbolMargin) + painter->drawLine(QLineF(x, y-skipSymbolMargin, x, b)); + } else + painter->drawLine(QLineF(x, a, x, b)); + // draw handles: + painter->drawLine(QLineF(x-barWidthHalf, a, x+barWidthHalf, a)); + painter->drawLine(QLineF(x-barWidthHalf, b, x+barWidthHalf, b)); + } + if (mErrorType == etValue || mErrorType == etBoth) + { + a = valueAxis->coordToPixel(data.value-data.valueErrorMinus); + b = valueAxis->coordToPixel(data.value+data.valueErrorPlus); + if (valueAxis->rangeReversed()) + qSwap(a,b); + // draw spine: + if (mErrorBarSkipSymbol) + { + if (x-a > skipSymbolMargin) // don't draw spine if error is so small it's within skipSymbolmargin + painter->drawLine(QLineF(a, y, x-skipSymbolMargin, y)); + if (b-x > skipSymbolMargin) + painter->drawLine(QLineF(x+skipSymbolMargin, y, b, y)); + } else + painter->drawLine(QLineF(a, y, b, y)); + // draw handles: + painter->drawLine(QLineF(a, y-barWidthHalf, a, y+barWidthHalf)); + painter->drawLine(QLineF(b, y-barWidthHalf, b, y+barWidthHalf)); + } + } else // mKeyAxis->orientation() is Qt::Horizontal + { + // draw value error vertically and key error horizontally + if (mErrorType == etKey || mErrorType == etBoth) + { + a = keyAxis->coordToPixel(data.key-data.keyErrorMinus); + b = keyAxis->coordToPixel(data.key+data.keyErrorPlus); + if (keyAxis->rangeReversed()) + qSwap(a,b); + // draw spine: + if (mErrorBarSkipSymbol) + { + if (x-a > skipSymbolMargin) // don't draw spine if error is so small it's within skipSymbolmargin + painter->drawLine(QLineF(a, y, x-skipSymbolMargin, y)); + if (b-x > skipSymbolMargin) + painter->drawLine(QLineF(x+skipSymbolMargin, y, b, y)); + } else + painter->drawLine(QLineF(a, y, b, y)); + // draw handles: + painter->drawLine(QLineF(a, y-barWidthHalf, a, y+barWidthHalf)); + painter->drawLine(QLineF(b, y-barWidthHalf, b, y+barWidthHalf)); + } + if (mErrorType == etValue || mErrorType == etBoth) + { + a = valueAxis->coordToPixel(data.value-data.valueErrorMinus); + b = valueAxis->coordToPixel(data.value+data.valueErrorPlus); + if (valueAxis->rangeReversed()) + qSwap(a,b); + // draw spine: + if (mErrorBarSkipSymbol) + { + if (a-y > skipSymbolMargin) // don't draw spine if error is so small it's within skipSymbolmargin + painter->drawLine(QLineF(x, a, x, y+skipSymbolMargin)); + if (y-b > skipSymbolMargin) + painter->drawLine(QLineF(x, y-skipSymbolMargin, x, b)); + } else + painter->drawLine(QLineF(x, a, x, b)); + // draw handles: + painter->drawLine(QLineF(x-barWidthHalf, a, x+barWidthHalf, a)); + painter->drawLine(QLineF(x-barWidthHalf, b, x+barWidthHalf, b)); + } + } +} + +/*! \internal + + called by \ref getPreparedData to determine which data (key) range is visible at the current key + axis range setting, so only that needs to be processed. + + \a lower returns an iterator to the lowest data point that needs to be taken into account when + plotting. Note that in order to get a clean plot all the way to the edge of the axis rect, \a + lower may still be just outside the visible range. + + \a upper returns an iterator to the highest data point. Same as before, \a upper may also lie + just outside of the visible range. + + if the graph contains no data, both \a lower and \a upper point to constEnd. +*/ +void QCPGraph::getVisibleDataBounds(QCPDataMap::const_iterator &lower, QCPDataMap::const_iterator &upper) const +{ + if (!mKeyAxis) { qDebug() << Q_FUNC_INFO << "invalid key axis"; return; } + if (mData->isEmpty()) + { + lower = mData->constEnd(); + upper = mData->constEnd(); + return; + } + + // get visible data range as QMap iterators + QCPDataMap::const_iterator lbound = mData->lowerBound(mKeyAxis.data()->range().lower); + QCPDataMap::const_iterator ubound = mData->upperBound(mKeyAxis.data()->range().upper); + bool lowoutlier = lbound != mData->constBegin(); // indicates whether there exist points below axis range + bool highoutlier = ubound != mData->constEnd(); // indicates whether there exist points above axis range + + lower = (lowoutlier ? lbound-1 : lbound); // data point range that will be actually drawn + upper = (highoutlier ? ubound : ubound-1); // data point range that will be actually drawn +} + +/*! \internal + + Counts the number of data points between \a lower and \a upper (including them), up to a maximum + of \a maxCount. + + This function is used by \ref getPreparedData to determine whether adaptive sampling shall be + used (if enabled via \ref setAdaptiveSampling) or not. This is also why counting of data points + only needs to be done until \a maxCount is reached, which should be set to the number of data + points at which adaptive sampling sets in. +*/ +int QCPGraph::countDataInBounds(const QCPDataMap::const_iterator &lower, const QCPDataMap::const_iterator &upper, int maxCount) const +{ + if (upper == mData->constEnd() && lower == mData->constEnd()) + return 0; + QCPDataMap::const_iterator it = lower; + int count = 1; + while (it != upper && count < maxCount) + { + ++it; + ++count; + } + return count; +} + +/*! \internal + + The line data vector generated by e.g. getLinePlotData contains only the line that connects the + data points. If the graph needs to be filled, two additional points need to be added at the + value-zero-line in the lower and upper key positions of the graph. This function calculates these + points and adds them to the end of \a lineData. Since the fill is typically drawn before the line + stroke, these added points need to be removed again after the fill is done, with the + removeFillBasePoints function. + + The expanding of \a lineData by two points will not cause unnecessary memory reallocations, + because the data vector generation functions (getLinePlotData etc.) reserve two extra points when + they allocate memory for \a lineData. + + \see removeFillBasePoints, lowerFillBasePoint, upperFillBasePoint +*/ +void QCPGraph::addFillBasePoints(QVector *lineData) const +{ + if (!mKeyAxis) { qDebug() << Q_FUNC_INFO << "invalid key axis"; return; } + if (!lineData) { qDebug() << Q_FUNC_INFO << "passed null as lineData"; return; } + if (lineData->isEmpty()) return; + + // append points that close the polygon fill at the key axis: + if (mKeyAxis.data()->orientation() == Qt::Vertical) + { + *lineData << upperFillBasePoint(lineData->last().y()); + *lineData << lowerFillBasePoint(lineData->first().y()); + } else + { + *lineData << upperFillBasePoint(lineData->last().x()); + *lineData << lowerFillBasePoint(lineData->first().x()); + } +} + +/*! \internal + + removes the two points from \a lineData that were added by \ref addFillBasePoints. + + \see addFillBasePoints, lowerFillBasePoint, upperFillBasePoint +*/ +void QCPGraph::removeFillBasePoints(QVector *lineData) const +{ + if (!lineData) { qDebug() << Q_FUNC_INFO << "passed null as lineData"; return; } + if (lineData->isEmpty()) return; + + lineData->remove(lineData->size()-2, 2); +} + +/*! \internal + + called by \ref addFillBasePoints to conveniently assign the point which closes the fill polygon + on the lower side of the zero-value-line parallel to the key axis. The logarithmic axis scale + case is a bit special, since the zero-value-line in pixel coordinates is in positive or negative + infinity. So this case is handled separately by just closing the fill polygon on the axis which + lies in the direction towards the zero value. + + \a lowerKey will be the the key (in pixels) of the returned point. Depending on whether the key + axis is horizontal or vertical, \a lowerKey will end up as the x or y value of the returned + point, respectively. + + \see upperFillBasePoint, addFillBasePoints +*/ +QPointF QCPGraph::lowerFillBasePoint(double lowerKey) const +{ + QCPAxis *keyAxis = mKeyAxis.data(); + QCPAxis *valueAxis = mValueAxis.data(); + if (!keyAxis || !valueAxis) { qDebug() << Q_FUNC_INFO << "invalid key or value axis"; return QPointF(); } + + QPointF point; + if (valueAxis->scaleType() == QCPAxis::stLinear) + { + if (keyAxis->axisType() == QCPAxis::atLeft) + { + point.setX(valueAxis->coordToPixel(0)); + point.setY(lowerKey); + } else if (keyAxis->axisType() == QCPAxis::atRight) + { + point.setX(valueAxis->coordToPixel(0)); + point.setY(lowerKey); + } else if (keyAxis->axisType() == QCPAxis::atTop) + { + point.setX(lowerKey); + point.setY(valueAxis->coordToPixel(0)); + } else if (keyAxis->axisType() == QCPAxis::atBottom) + { + point.setX(lowerKey); + point.setY(valueAxis->coordToPixel(0)); + } + } else // valueAxis->mScaleType == QCPAxis::stLogarithmic + { + // In logarithmic scaling we can't just draw to value zero so we just fill all the way + // to the axis which is in the direction towards zero + if (keyAxis->orientation() == Qt::Vertical) + { + if ((valueAxis->range().upper < 0 && !valueAxis->rangeReversed()) || + (valueAxis->range().upper > 0 && valueAxis->rangeReversed())) // if range is negative, zero is on opposite side of key axis + point.setX(keyAxis->axisRect()->right()); + else + point.setX(keyAxis->axisRect()->left()); + point.setY(lowerKey); + } else if (keyAxis->axisType() == QCPAxis::atTop || keyAxis->axisType() == QCPAxis::atBottom) + { + point.setX(lowerKey); + if ((valueAxis->range().upper < 0 && !valueAxis->rangeReversed()) || + (valueAxis->range().upper > 0 && valueAxis->rangeReversed())) // if range is negative, zero is on opposite side of key axis + point.setY(keyAxis->axisRect()->top()); + else + point.setY(keyAxis->axisRect()->bottom()); + } + } + return point; +} + +/*! \internal + + called by \ref addFillBasePoints to conveniently assign the point which closes the fill + polygon on the upper side of the zero-value-line parallel to the key axis. The logarithmic axis + scale case is a bit special, since the zero-value-line in pixel coordinates is in positive or + negative infinity. So this case is handled separately by just closing the fill polygon on the + axis which lies in the direction towards the zero value. + + \a upperKey will be the the key (in pixels) of the returned point. Depending on whether the key + axis is horizontal or vertical, \a upperKey will end up as the x or y value of the returned + point, respectively. + + \see lowerFillBasePoint, addFillBasePoints +*/ +QPointF QCPGraph::upperFillBasePoint(double upperKey) const +{ + QCPAxis *keyAxis = mKeyAxis.data(); + QCPAxis *valueAxis = mValueAxis.data(); + if (!keyAxis || !valueAxis) { qDebug() << Q_FUNC_INFO << "invalid key or value axis"; return QPointF(); } + + QPointF point; + if (valueAxis->scaleType() == QCPAxis::stLinear) + { + if (keyAxis->axisType() == QCPAxis::atLeft) + { + point.setX(valueAxis->coordToPixel(0)); + point.setY(upperKey); + } else if (keyAxis->axisType() == QCPAxis::atRight) + { + point.setX(valueAxis->coordToPixel(0)); + point.setY(upperKey); + } else if (keyAxis->axisType() == QCPAxis::atTop) + { + point.setX(upperKey); + point.setY(valueAxis->coordToPixel(0)); + } else if (keyAxis->axisType() == QCPAxis::atBottom) + { + point.setX(upperKey); + point.setY(valueAxis->coordToPixel(0)); + } + } else // valueAxis->mScaleType == QCPAxis::stLogarithmic + { + // In logarithmic scaling we can't just draw to value 0 so we just fill all the way + // to the axis which is in the direction towards 0 + if (keyAxis->orientation() == Qt::Vertical) + { + if ((valueAxis->range().upper < 0 && !valueAxis->rangeReversed()) || + (valueAxis->range().upper > 0 && valueAxis->rangeReversed())) // if range is negative, zero is on opposite side of key axis + point.setX(keyAxis->axisRect()->right()); + else + point.setX(keyAxis->axisRect()->left()); + point.setY(upperKey); + } else if (keyAxis->axisType() == QCPAxis::atTop || keyAxis->axisType() == QCPAxis::atBottom) + { + point.setX(upperKey); + if ((valueAxis->range().upper < 0 && !valueAxis->rangeReversed()) || + (valueAxis->range().upper > 0 && valueAxis->rangeReversed())) // if range is negative, zero is on opposite side of key axis + point.setY(keyAxis->axisRect()->top()); + else + point.setY(keyAxis->axisRect()->bottom()); + } + } + return point; +} + +/*! \internal + + Generates the polygon needed for drawing channel fills between this graph (data passed via \a + lineData) and the graph specified by mChannelFillGraph (data generated by calling its \ref + getPlotData function). May return an empty polygon if the key ranges have no overlap or fill + target graph and this graph don't have same orientation (i.e. both key axes horizontal or both + key axes vertical). For increased performance (due to implicit sharing), keep the returned + QPolygonF const. +*/ +const QPolygonF QCPGraph::getChannelFillPolygon(const QVector *lineData) const +{ + if (!mChannelFillGraph) + return QPolygonF(); + + QCPAxis *keyAxis = mKeyAxis.data(); + QCPAxis *valueAxis = mValueAxis.data(); + if (!keyAxis || !valueAxis) { qDebug() << Q_FUNC_INFO << "invalid key or value axis"; return QPolygonF(); } + if (!mChannelFillGraph.data()->mKeyAxis) { qDebug() << Q_FUNC_INFO << "channel fill target key axis invalid"; return QPolygonF(); } + + if (mChannelFillGraph.data()->mKeyAxis.data()->orientation() != keyAxis->orientation()) + return QPolygonF(); // don't have same axis orientation, can't fill that (Note: if keyAxis fits, valueAxis will fit too, because it's always orthogonal to keyAxis) + + if (lineData->isEmpty()) return QPolygonF(); + QVector otherData; + mChannelFillGraph.data()->getPlotData(&otherData, 0); + if (otherData.isEmpty()) return QPolygonF(); + QVector thisData; + thisData.reserve(lineData->size()+otherData.size()); // because we will join both vectors at end of this function + for (int i=0; isize(); ++i) // don't use the vector<<(vector), it squeezes internally, which ruins the performance tuning with reserve() + thisData << lineData->at(i); + + // pointers to be able to swap them, depending which data range needs cropping: + QVector *staticData = &thisData; + QVector *croppedData = &otherData; + + // crop both vectors to ranges in which the keys overlap (which coord is key, depends on axisType): + if (keyAxis->orientation() == Qt::Horizontal) + { + // x is key + // if an axis range is reversed, the data point keys will be descending. Reverse them, since following algorithm assumes ascending keys: + if (staticData->first().x() > staticData->last().x()) + { + int size = staticData->size(); + for (int i=0; ifirst().x() > croppedData->last().x()) + { + int size = croppedData->size(); + for (int i=0; ifirst().x() < croppedData->first().x()) // other one must be cropped + qSwap(staticData, croppedData); + int lowBound = findIndexBelowX(croppedData, staticData->first().x()); + if (lowBound == -1) return QPolygonF(); // key ranges have no overlap + croppedData->remove(0, lowBound); + // set lowest point of cropped data to fit exactly key position of first static data + // point via linear interpolation: + if (croppedData->size() < 2) return QPolygonF(); // need at least two points for interpolation + double slope; + if (croppedData->at(1).x()-croppedData->at(0).x() != 0) + slope = (croppedData->at(1).y()-croppedData->at(0).y())/(croppedData->at(1).x()-croppedData->at(0).x()); + else + slope = 0; + (*croppedData)[0].setY(croppedData->at(0).y()+slope*(staticData->first().x()-croppedData->at(0).x())); + (*croppedData)[0].setX(staticData->first().x()); + + // crop upper bound: + if (staticData->last().x() > croppedData->last().x()) // other one must be cropped + qSwap(staticData, croppedData); + int highBound = findIndexAboveX(croppedData, staticData->last().x()); + if (highBound == -1) return QPolygonF(); // key ranges have no overlap + croppedData->remove(highBound+1, croppedData->size()-(highBound+1)); + // set highest point of cropped data to fit exactly key position of last static data + // point via linear interpolation: + if (croppedData->size() < 2) return QPolygonF(); // need at least two points for interpolation + int li = croppedData->size()-1; // last index + if (croppedData->at(li).x()-croppedData->at(li-1).x() != 0) + slope = (croppedData->at(li).y()-croppedData->at(li-1).y())/(croppedData->at(li).x()-croppedData->at(li-1).x()); + else + slope = 0; + (*croppedData)[li].setY(croppedData->at(li-1).y()+slope*(staticData->last().x()-croppedData->at(li-1).x())); + (*croppedData)[li].setX(staticData->last().x()); + } else // mKeyAxis->orientation() == Qt::Vertical + { + // y is key + // similar to "x is key" but switched x,y. Further, lower/upper meaning is inverted compared to x, + // because in pixel coordinates, y increases from top to bottom, not bottom to top like data coordinate. + // if an axis range is reversed, the data point keys will be descending. Reverse them, since following algorithm assumes ascending keys: + if (staticData->first().y() < staticData->last().y()) + { + int size = staticData->size(); + for (int i=0; ifirst().y() < croppedData->last().y()) + { + int size = croppedData->size(); + for (int i=0; ifirst().y() > croppedData->first().y()) // other one must be cropped + qSwap(staticData, croppedData); + int lowBound = findIndexAboveY(croppedData, staticData->first().y()); + if (lowBound == -1) return QPolygonF(); // key ranges have no overlap + croppedData->remove(0, lowBound); + // set lowest point of cropped data to fit exactly key position of first static data + // point via linear interpolation: + if (croppedData->size() < 2) return QPolygonF(); // need at least two points for interpolation + double slope; + if (croppedData->at(1).y()-croppedData->at(0).y() != 0) // avoid division by zero in step plots + slope = (croppedData->at(1).x()-croppedData->at(0).x())/(croppedData->at(1).y()-croppedData->at(0).y()); + else + slope = 0; + (*croppedData)[0].setX(croppedData->at(0).x()+slope*(staticData->first().y()-croppedData->at(0).y())); + (*croppedData)[0].setY(staticData->first().y()); + + // crop upper bound: + if (staticData->last().y() < croppedData->last().y()) // other one must be cropped + qSwap(staticData, croppedData); + int highBound = findIndexBelowY(croppedData, staticData->last().y()); + if (highBound == -1) return QPolygonF(); // key ranges have no overlap + croppedData->remove(highBound+1, croppedData->size()-(highBound+1)); + // set highest point of cropped data to fit exactly key position of last static data + // point via linear interpolation: + if (croppedData->size() < 2) return QPolygonF(); // need at least two points for interpolation + int li = croppedData->size()-1; // last index + if (croppedData->at(li).y()-croppedData->at(li-1).y() != 0) // avoid division by zero in step plots + slope = (croppedData->at(li).x()-croppedData->at(li-1).x())/(croppedData->at(li).y()-croppedData->at(li-1).y()); + else + slope = 0; + (*croppedData)[li].setX(croppedData->at(li-1).x()+slope*(staticData->last().y()-croppedData->at(li-1).y())); + (*croppedData)[li].setY(staticData->last().y()); + } + + // return joined: + for (int i=otherData.size()-1; i>=0; --i) // insert reversed, otherwise the polygon will be twisted + thisData << otherData.at(i); + return QPolygonF(thisData); +} + +/*! \internal + + Finds the smallest index of \a data, whose points x value is just above \a x. Assumes x values in + \a data points are ordered ascending, as is the case when plotting with horizontal key axis. + + Used to calculate the channel fill polygon, see \ref getChannelFillPolygon. +*/ +int QCPGraph::findIndexAboveX(const QVector *data, double x) const +{ + for (int i=data->size()-1; i>=0; --i) + { + if (data->at(i).x() < x) + { + if (isize()-1) + return i+1; + else + return data->size()-1; + } + } + return -1; +} + +/*! \internal + + Finds the highest index of \a data, whose points x value is just below \a x. Assumes x values in + \a data points are ordered ascending, as is the case when plotting with horizontal key axis. + + Used to calculate the channel fill polygon, see \ref getChannelFillPolygon. +*/ +int QCPGraph::findIndexBelowX(const QVector *data, double x) const +{ + for (int i=0; isize(); ++i) + { + if (data->at(i).x() > x) + { + if (i>0) + return i-1; + else + return 0; + } + } + return -1; +} + +/*! \internal + + Finds the smallest index of \a data, whose points y value is just above \a y. Assumes y values in + \a data points are ordered descending, as is the case when plotting with vertical key axis. + + Used to calculate the channel fill polygon, see \ref getChannelFillPolygon. +*/ +int QCPGraph::findIndexAboveY(const QVector *data, double y) const +{ + for (int i=0; isize(); ++i) + { + if (data->at(i).y() < y) + { + if (i>0) + return i-1; + else + return 0; + } + } + return -1; +} + +/*! \internal + + Calculates the (minimum) distance (in pixels) the graph's representation has from the given \a + pixelPoint in pixels. This is used to determine whether the graph was clicked or not, e.g. in + \ref selectTest. + + If either the graph has no data or if the line style is \ref lsNone and the scatter style's shape + is \ref QCPScatterStyle::ssNone (i.e. there is no visual representation of the graph), returns -1.0. +*/ +double QCPGraph::pointDistance(const QPointF &pixelPoint) const +{ + if (mData->isEmpty()) + return -1.0; + if (mLineStyle == lsNone && mScatterStyle.isNone()) + return -1.0; + + // calculate minimum distances to graph representation: + if (mLineStyle == lsNone) + { + // no line displayed, only calculate distance to scatter points: + QVector scatterData; + getScatterPlotData(&scatterData); + if (scatterData.size() > 0) + { + double minDistSqr = std::numeric_limits::max(); + for (int i=0; i lineData; + getPlotData(&lineData, 0); // unlike with getScatterPlotData we get pixel coordinates here + if (lineData.size() > 1) // at least one line segment, compare distance to line segments + { + double minDistSqr = std::numeric_limits::max(); + if (mLineStyle == lsImpulse) + { + // impulse plot differs from other line styles in that the lineData points are only pairwise connected: + for (int i=0; i 0) // only single data point, calculate distance to that point + { + return QVector2D(lineData.at(0)-pixelPoint).length(); + } else // no data available in view to calculate distance to + return -1.0; + } +} + +/*! \internal + + Finds the highest index of \a data, whose points y value is just below \a y. Assumes y values in + \a data points are ordered descending, as is the case when plotting with vertical key axis (since + keys are ordered ascending). + + Used to calculate the channel fill polygon, see \ref getChannelFillPolygon. +*/ +int QCPGraph::findIndexBelowY(const QVector *data, double y) const +{ + for (int i=data->size()-1; i>=0; --i) + { + if (data->at(i).y() > y) + { + if (isize()-1) + return i+1; + else + return data->size()-1; + } + } + return -1; +} + +/* inherits documentation from base class */ +QCPRange QCPGraph::getKeyRange(bool &foundRange, SignDomain inSignDomain) const +{ + // just call the specialized version which takes an additional argument whether error bars + // should also be taken into consideration for range calculation. We set this to true here. + return getKeyRange(foundRange, inSignDomain, true); +} + +/* inherits documentation from base class */ +QCPRange QCPGraph::getValueRange(bool &foundRange, SignDomain inSignDomain) const +{ + // just call the specialized version which takes an additional argument whether error bars + // should also be taken into consideration for range calculation. We set this to true here. + return getValueRange(foundRange, inSignDomain, true); +} + +/*! \overload + + Allows to specify whether the error bars should be included in the range calculation. + + \see getKeyRange(bool &foundRange, SignDomain inSignDomain) +*/ +QCPRange QCPGraph::getKeyRange(bool &foundRange, SignDomain inSignDomain, bool includeErrors) const +{ + QCPRange range; + bool haveLower = false; + bool haveUpper = false; + + double current, currentErrorMinus, currentErrorPlus; + + if (inSignDomain == sdBoth) // range may be anywhere + { + QCPDataMap::const_iterator it = mData->constBegin(); + while (it != mData->constEnd()) + { + if (!qIsNaN(it.value().value)) + { + current = it.value().key; + currentErrorMinus = (includeErrors ? it.value().keyErrorMinus : 0); + currentErrorPlus = (includeErrors ? it.value().keyErrorPlus : 0); + if (current-currentErrorMinus < range.lower || !haveLower) + { + range.lower = current-currentErrorMinus; + haveLower = true; + } + if (current+currentErrorPlus > range.upper || !haveUpper) + { + range.upper = current+currentErrorPlus; + haveUpper = true; + } + } + ++it; + } + } else if (inSignDomain == sdNegative) // range may only be in the negative sign domain + { + QCPDataMap::const_iterator it = mData->constBegin(); + while (it != mData->constEnd()) + { + if (!qIsNaN(it.value().value)) + { + current = it.value().key; + currentErrorMinus = (includeErrors ? it.value().keyErrorMinus : 0); + currentErrorPlus = (includeErrors ? it.value().keyErrorPlus : 0); + if ((current-currentErrorMinus < range.lower || !haveLower) && current-currentErrorMinus < 0) + { + range.lower = current-currentErrorMinus; + haveLower = true; + } + if ((current+currentErrorPlus > range.upper || !haveUpper) && current+currentErrorPlus < 0) + { + range.upper = current+currentErrorPlus; + haveUpper = true; + } + if (includeErrors) // in case point is in valid sign domain but errobars stretch beyond it, we still want to geht that point. + { + if ((current < range.lower || !haveLower) && current < 0) + { + range.lower = current; + haveLower = true; + } + if ((current > range.upper || !haveUpper) && current < 0) + { + range.upper = current; + haveUpper = true; + } + } + } + ++it; + } + } else if (inSignDomain == sdPositive) // range may only be in the positive sign domain + { + QCPDataMap::const_iterator it = mData->constBegin(); + while (it != mData->constEnd()) + { + if (!qIsNaN(it.value().value)) + { + current = it.value().key; + currentErrorMinus = (includeErrors ? it.value().keyErrorMinus : 0); + currentErrorPlus = (includeErrors ? it.value().keyErrorPlus : 0); + if ((current-currentErrorMinus < range.lower || !haveLower) && current-currentErrorMinus > 0) + { + range.lower = current-currentErrorMinus; + haveLower = true; + } + if ((current+currentErrorPlus > range.upper || !haveUpper) && current+currentErrorPlus > 0) + { + range.upper = current+currentErrorPlus; + haveUpper = true; + } + if (includeErrors) // in case point is in valid sign domain but errobars stretch beyond it, we still want to get that point. + { + if ((current < range.lower || !haveLower) && current > 0) + { + range.lower = current; + haveLower = true; + } + if ((current > range.upper || !haveUpper) && current > 0) + { + range.upper = current; + haveUpper = true; + } + } + } + ++it; + } + } + + foundRange = haveLower && haveUpper; + return range; +} + +/*! \overload + + Allows to specify whether the error bars should be included in the range calculation. + + \see getValueRange(bool &foundRange, SignDomain inSignDomain) +*/ +QCPRange QCPGraph::getValueRange(bool &foundRange, SignDomain inSignDomain, bool includeErrors) const +{ + QCPRange range; + bool haveLower = false; + bool haveUpper = false; + + double current, currentErrorMinus, currentErrorPlus; + + if (inSignDomain == sdBoth) // range may be anywhere + { + QCPDataMap::const_iterator it = mData->constBegin(); + while (it != mData->constEnd()) + { + current = it.value().value; + if (!qIsNaN(current)) + { + currentErrorMinus = (includeErrors ? it.value().valueErrorMinus : 0); + currentErrorPlus = (includeErrors ? it.value().valueErrorPlus : 0); + if (current-currentErrorMinus < range.lower || !haveLower) + { + range.lower = current-currentErrorMinus; + haveLower = true; + } + if (current+currentErrorPlus > range.upper || !haveUpper) + { + range.upper = current+currentErrorPlus; + haveUpper = true; + } + } + ++it; + } + } else if (inSignDomain == sdNegative) // range may only be in the negative sign domain + { + QCPDataMap::const_iterator it = mData->constBegin(); + while (it != mData->constEnd()) + { + current = it.value().value; + if (!qIsNaN(current)) + { + currentErrorMinus = (includeErrors ? it.value().valueErrorMinus : 0); + currentErrorPlus = (includeErrors ? it.value().valueErrorPlus : 0); + if ((current-currentErrorMinus < range.lower || !haveLower) && current-currentErrorMinus < 0) + { + range.lower = current-currentErrorMinus; + haveLower = true; + } + if ((current+currentErrorPlus > range.upper || !haveUpper) && current+currentErrorPlus < 0) + { + range.upper = current+currentErrorPlus; + haveUpper = true; + } + if (includeErrors) // in case point is in valid sign domain but errobars stretch beyond it, we still want to get that point. + { + if ((current < range.lower || !haveLower) && current < 0) + { + range.lower = current; + haveLower = true; + } + if ((current > range.upper || !haveUpper) && current < 0) + { + range.upper = current; + haveUpper = true; + } + } + } + ++it; + } + } else if (inSignDomain == sdPositive) // range may only be in the positive sign domain + { + QCPDataMap::const_iterator it = mData->constBegin(); + while (it != mData->constEnd()) + { + current = it.value().value; + if (!qIsNaN(current)) + { + currentErrorMinus = (includeErrors ? it.value().valueErrorMinus : 0); + currentErrorPlus = (includeErrors ? it.value().valueErrorPlus : 0); + if ((current-currentErrorMinus < range.lower || !haveLower) && current-currentErrorMinus > 0) + { + range.lower = current-currentErrorMinus; + haveLower = true; + } + if ((current+currentErrorPlus > range.upper || !haveUpper) && current+currentErrorPlus > 0) + { + range.upper = current+currentErrorPlus; + haveUpper = true; + } + if (includeErrors) // in case point is in valid sign domain but errobars stretch beyond it, we still want to geht that point. + { + if ((current < range.lower || !haveLower) && current > 0) + { + range.lower = current; + haveLower = true; + } + if ((current > range.upper || !haveUpper) && current > 0) + { + range.upper = current; + haveUpper = true; + } + } + } + ++it; + } + } + + foundRange = haveLower && haveUpper; + return range; +} + + +//////////////////////////////////////////////////////////////////////////////////////////////////// +//////////////////// QCPCurveData +//////////////////////////////////////////////////////////////////////////////////////////////////// + +/*! \class QCPCurveData + \brief Holds the data of one single data point for QCPCurve. + + The container for storing multiple data points is \ref QCPCurveDataMap. + + The stored data is: + \li \a t: the free parameter of the curve at this curve point (cp. the mathematical vector (x(t), y(t))) + \li \a key: coordinate on the key axis of this curve point + \li \a value: coordinate on the value axis of this curve point + + \see QCPCurveDataMap +*/ + +/*! + Constructs a curve data point with t, key and value set to zero. +*/ +QCPCurveData::QCPCurveData() : + t(0), + key(0), + value(0) +{ +} + +/*! + Constructs a curve data point with the specified \a t, \a key and \a value. +*/ +QCPCurveData::QCPCurveData(double t, double key, double value) : + t(t), + key(key), + value(value) +{ +} + + +//////////////////////////////////////////////////////////////////////////////////////////////////// +//////////////////// QCPCurve +//////////////////////////////////////////////////////////////////////////////////////////////////// + +/*! \class QCPCurve + \brief A plottable representing a parametric curve in a plot. + + \image html QCPCurve.png + + Unlike QCPGraph, plottables of this type may have multiple points with the same key coordinate, + so their visual representation can have \a loops. This is realized by introducing a third + coordinate \a t, which defines the order of the points described by the other two coordinates \a + x and \a y. + + To plot data, assign it with the \ref setData or \ref addData functions. + + Gaps in the curve can be created by adding data points with NaN as key and value + (qQNaN() or std::numeric_limits::quiet_NaN()) in between the two data points that shall be + separated. + + \section appearance Changing the appearance + + The appearance of the curve is determined by the pen and the brush (\ref setPen, \ref setBrush). + \section usage Usage + + Like all data representing objects in QCustomPlot, the QCPCurve is a plottable (QCPAbstractPlottable). So + the plottable-interface of QCustomPlot applies (QCustomPlot::plottable, QCustomPlot::addPlottable, QCustomPlot::removePlottable, etc.) + + Usually, you first create an instance and add it to the customPlot: + \snippet documentation/doc-code-snippets/mainwindow.cpp qcpcurve-creation-1 + and then modify the properties of the newly created plottable, e.g.: + \snippet documentation/doc-code-snippets/mainwindow.cpp qcpcurve-creation-2 +*/ + +/*! + Constructs a curve which uses \a keyAxis as its key axis ("x") and \a valueAxis as its value + axis ("y"). \a keyAxis and \a valueAxis must reside in the same QCustomPlot instance and not have + the same orientation. If either of these restrictions is violated, a corresponding message is + printed to the debug output (qDebug), the construction is not aborted, though. + + The constructed QCPCurve can be added to the plot with QCustomPlot::addPlottable, QCustomPlot + then takes ownership of the graph. +*/ +QCPCurve::QCPCurve(QCPAxis *keyAxis, QCPAxis *valueAxis) : + QCPAbstractPlottable(keyAxis, valueAxis) +{ + mData = new QCPCurveDataMap; + mPen.setColor(Qt::blue); + mPen.setStyle(Qt::SolidLine); + mBrush.setColor(Qt::blue); + mBrush.setStyle(Qt::NoBrush); + mSelectedPen = mPen; + mSelectedPen.setWidthF(2.5); + mSelectedPen.setColor(QColor(80, 80, 255)); // lighter than Qt::blue of mPen + mSelectedBrush = mBrush; + + setScatterStyle(QCPScatterStyle()); + setLineStyle(lsLine); +} + +QCPCurve::~QCPCurve() +{ + delete mData; +} + +/*! + Replaces the current data with the provided \a data. + + If \a copy is set to true, data points in \a data will only be copied. if false, the plottable + takes ownership of the passed data and replaces the internal data pointer with it. This is + significantly faster than copying for large datasets. +*/ +void QCPCurve::setData(QCPCurveDataMap *data, bool copy) +{ + if (mData == data) + { + qDebug() << Q_FUNC_INFO << "The data pointer is already in (and owned by) this plottable" << reinterpret_cast(data); + return; + } + if (copy) + { + *mData = *data; + } else + { + delete mData; + mData = data; + } +} + +/*! \overload + + Replaces the current data with the provided points in \a t, \a key and \a value tuples. The + provided vectors should have equal length. Else, the number of added points will be the size of + the smallest vector. +*/ +void QCPCurve::setData(const QVector &t, const QVector &key, const QVector &value) +{ + mData->clear(); + int n = t.size(); + n = qMin(n, key.size()); + n = qMin(n, value.size()); + QCPCurveData newData; + for (int i=0; iinsertMulti(newData.t, newData); + } +} + +/*! \overload + + Replaces the current data with the provided \a key and \a value pairs. The t parameter + of each data point will be set to the integer index of the respective key/value pair. +*/ +void QCPCurve::setData(const QVector &key, const QVector &value) +{ + mData->clear(); + int n = key.size(); + n = qMin(n, value.size()); + QCPCurveData newData; + for (int i=0; iinsertMulti(newData.t, newData); + } +} + +/*! + Sets the visual appearance of single data points in the plot. If set to \ref + QCPScatterStyle::ssNone, no scatter points are drawn (e.g. for line-only plots with appropriate + line style). + + \see QCPScatterStyle, setLineStyle +*/ +void QCPCurve::setScatterStyle(const QCPScatterStyle &style) +{ + mScatterStyle = style; +} + +/*! + Sets how the single data points are connected in the plot or how they are represented visually + apart from the scatter symbol. For scatter-only plots, set \a style to \ref lsNone and \ref + setScatterStyle to the desired scatter style. + + \see setScatterStyle +*/ +void QCPCurve::setLineStyle(QCPCurve::LineStyle style) +{ + mLineStyle = style; +} + +/*! + Adds the provided data points in \a dataMap to the current data. + \see removeData +*/ +void QCPCurve::addData(const QCPCurveDataMap &dataMap) +{ + mData->unite(dataMap); +} + +/*! \overload + Adds the provided single data point in \a data to the current data. + \see removeData +*/ +void QCPCurve::addData(const QCPCurveData &data) +{ + mData->insertMulti(data.t, data); +} + +/*! \overload + Adds the provided single data point as \a t, \a key and \a value tuple to the current data + \see removeData +*/ +void QCPCurve::addData(double t, double key, double value) +{ + QCPCurveData newData; + newData.t = t; + newData.key = key; + newData.value = value; + mData->insertMulti(newData.t, newData); +} + +/*! \overload + + Adds the provided single data point as \a key and \a value pair to the current data The t + parameter of the data point is set to the t of the last data point plus 1. If there is no last + data point, t will be set to 0. + + \see removeData +*/ +void QCPCurve::addData(double key, double value) +{ + QCPCurveData newData; + if (!mData->isEmpty()) + newData.t = (mData->constEnd()-1).key()+1; + else + newData.t = 0; + newData.key = key; + newData.value = value; + mData->insertMulti(newData.t, newData); +} + +/*! \overload + Adds the provided data points as \a t, \a key and \a value tuples to the current data. + \see removeData +*/ +void QCPCurve::addData(const QVector &ts, const QVector &keys, const QVector &values) +{ + int n = ts.size(); + n = qMin(n, keys.size()); + n = qMin(n, values.size()); + QCPCurveData newData; + for (int i=0; iinsertMulti(newData.t, newData); + } +} + +/*! + Removes all data points with curve parameter t smaller than \a t. + \see addData, clearData +*/ +void QCPCurve::removeDataBefore(double t) +{ + QCPCurveDataMap::iterator it = mData->begin(); + while (it != mData->end() && it.key() < t) + it = mData->erase(it); +} + +/*! + Removes all data points with curve parameter t greater than \a t. + \see addData, clearData +*/ +void QCPCurve::removeDataAfter(double t) +{ + if (mData->isEmpty()) return; + QCPCurveDataMap::iterator it = mData->upperBound(t); + while (it != mData->end()) + it = mData->erase(it); +} + +/*! + Removes all data points with curve parameter t between \a fromt and \a tot. if \a fromt is + greater or equal to \a tot, the function does nothing. To remove a single data point with known + t, use \ref removeData(double t). + + \see addData, clearData +*/ +void QCPCurve::removeData(double fromt, double tot) +{ + if (fromt >= tot || mData->isEmpty()) return; + QCPCurveDataMap::iterator it = mData->upperBound(fromt); + QCPCurveDataMap::iterator itEnd = mData->upperBound(tot); + while (it != itEnd) + it = mData->erase(it); +} + +/*! \overload + + Removes a single data point at curve parameter \a t. If the position is not known with absolute + precision, consider using \ref removeData(double fromt, double tot) with a small fuzziness + interval around the suspected position, depeding on the precision with which the curve parameter + is known. + + \see addData, clearData +*/ +void QCPCurve::removeData(double t) +{ + mData->remove(t); +} + +/*! + Removes all data points. + \see removeData, removeDataAfter, removeDataBefore +*/ +void QCPCurve::clearData() +{ + mData->clear(); +} + +/* inherits documentation from base class */ +double QCPCurve::selectTest(const QPointF &pos, bool onlySelectable, QVariant *details) const +{ + Q_UNUSED(details) + if ((onlySelectable && !mSelectable) || mData->isEmpty()) + return -1; + if (!mKeyAxis || !mValueAxis) { qDebug() << Q_FUNC_INFO << "invalid key or value axis"; return -1; } + + if (mKeyAxis.data()->axisRect()->rect().contains(pos.toPoint())) + return pointDistance(pos); + else + return -1; +} + +/* inherits documentation from base class */ +void QCPCurve::draw(QCPPainter *painter) +{ + if (mData->isEmpty()) return; + + // allocate line vector: + QVector *lineData = new QVector; + + // fill with curve data: + getCurveData(lineData); + + // check data validity if flag set: +#ifdef QCUSTOMPLOT_CHECK_DATA + QCPCurveDataMap::const_iterator it; + for (it = mData->constBegin(); it != mData->constEnd(); ++it) + { + if (QCP::isInvalidData(it.value().t) || + QCP::isInvalidData(it.value().key, it.value().value)) + qDebug() << Q_FUNC_INFO << "Data point at" << it.key() << "invalid." << "Plottable name:" << name(); + } +#endif + + // draw curve fill: + if (mainBrush().style() != Qt::NoBrush && mainBrush().color().alpha() != 0) + { + applyFillAntialiasingHint(painter); + painter->setPen(Qt::NoPen); + painter->setBrush(mainBrush()); + painter->drawPolygon(QPolygonF(*lineData)); + } + + // draw curve line: + if (mLineStyle != lsNone && mainPen().style() != Qt::NoPen && mainPen().color().alpha() != 0) + { + applyDefaultAntialiasingHint(painter); + painter->setPen(mainPen()); + painter->setBrush(Qt::NoBrush); + // if drawing solid line and not in PDF, use much faster line drawing instead of polyline: + if (mParentPlot->plottingHints().testFlag(QCP::phFastPolylines) && + painter->pen().style() == Qt::SolidLine && + !painter->modes().testFlag(QCPPainter::pmVectorized) && + !painter->modes().testFlag(QCPPainter::pmNoCaching)) + { + int i = 0; + bool lastIsNan = false; + const int lineDataSize = lineData->size(); + while (i < lineDataSize && (qIsNaN(lineData->at(i).y()) || qIsNaN(lineData->at(i).x()))) // make sure first point is not NaN + ++i; + ++i; // because drawing works in 1 point retrospect + while (i < lineDataSize) + { + if (!qIsNaN(lineData->at(i).y()) && !qIsNaN(lineData->at(i).x())) // NaNs create a gap in the line + { + if (!lastIsNan) + painter->drawLine(lineData->at(i-1), lineData->at(i)); + else + lastIsNan = false; + } else + lastIsNan = true; + ++i; + } + } else + { + int segmentStart = 0; + int i = 0; + const int lineDataSize = lineData->size(); + while (i < lineDataSize) + { + if (qIsNaN(lineData->at(i).y()) || qIsNaN(lineData->at(i).x())) // NaNs create a gap in the line + { + painter->drawPolyline(lineData->constData()+segmentStart, i-segmentStart); // i, because we don't want to include the current NaN point + segmentStart = i+1; + } + ++i; + } + // draw last segment: + painter->drawPolyline(lineData->constData()+segmentStart, lineDataSize-segmentStart); + } + } + + // draw scatters: + if (!mScatterStyle.isNone()) + drawScatterPlot(painter, lineData); + + // free allocated line data: + delete lineData; +} + +/* inherits documentation from base class */ +void QCPCurve::drawLegendIcon(QCPPainter *painter, const QRectF &rect) const +{ + // draw fill: + if (mBrush.style() != Qt::NoBrush) + { + applyFillAntialiasingHint(painter); + painter->fillRect(QRectF(rect.left(), rect.top()+rect.height()/2.0, rect.width(), rect.height()/3.0), mBrush); + } + // draw line vertically centered: + if (mLineStyle != lsNone) + { + applyDefaultAntialiasingHint(painter); + painter->setPen(mPen); + painter->drawLine(QLineF(rect.left(), rect.top()+rect.height()/2.0, rect.right()+5, rect.top()+rect.height()/2.0)); // +5 on x2 else last segment is missing from dashed/dotted pens + } + // draw scatter symbol: + if (!mScatterStyle.isNone()) + { + applyScattersAntialiasingHint(painter); + // scale scatter pixmap if it's too large to fit in legend icon rect: + if (mScatterStyle.shape() == QCPScatterStyle::ssPixmap && (mScatterStyle.pixmap().size().width() > rect.width() || mScatterStyle.pixmap().size().height() > rect.height())) + { + QCPScatterStyle scaledStyle(mScatterStyle); + scaledStyle.setPixmap(scaledStyle.pixmap().scaled(rect.size().toSize(), Qt::KeepAspectRatio, Qt::SmoothTransformation)); + scaledStyle.applyTo(painter, mPen); + scaledStyle.drawShape(painter, QRectF(rect).center()); + } else + { + mScatterStyle.applyTo(painter, mPen); + mScatterStyle.drawShape(painter, QRectF(rect).center()); + } + } +} + +/*! \internal + + Draws scatter symbols at every data point passed in \a pointData. scatter symbols are independent of + the line style and are always drawn if scatter shape is not \ref QCPScatterStyle::ssNone. +*/ +void QCPCurve::drawScatterPlot(QCPPainter *painter, const QVector *pointData) const +{ + // draw scatter point symbols: + applyScattersAntialiasingHint(painter); + mScatterStyle.applyTo(painter, mPen); + for (int i=0; isize(); ++i) + if (!qIsNaN(pointData->at(i).x()) && !qIsNaN(pointData->at(i).y())) + mScatterStyle.drawShape(painter, pointData->at(i)); +} + +/*! \internal + + called by QCPCurve::draw to generate a point vector (in pixel coordinates) which represents the + line of the curve. + + Line segments that aren't visible in the current axis rect are handled in an optimized way. They + are projected onto a rectangle slightly larger than the visible axis rect and simplified + regarding point count. The algorithm makes sure to preserve appearance of lines and fills inside + the visible axis rect by generating new temporary points on the outer rect if necessary. + + Methods that are also involved in the algorithm are: \ref getRegion, \ref getOptimizedPoint, \ref + getOptimizedCornerPoints \ref mayTraverse, \ref getTraverse, \ref getTraverseCornerPoints. +*/ +void QCPCurve::getCurveData(QVector *lineData) const +{ + QCPAxis *keyAxis = mKeyAxis.data(); + QCPAxis *valueAxis = mValueAxis.data(); + if (!keyAxis || !valueAxis) { qDebug() << Q_FUNC_INFO << "invalid key or value axis"; return; } + + // add margins to rect to compensate for stroke width + double strokeMargin = qMax(qreal(1.0), qreal(mainPen().widthF()*0.75)); // stroke radius + 50% safety + if (!mScatterStyle.isNone()) + strokeMargin = qMax(strokeMargin, mScatterStyle.size()); + double rectLeft = keyAxis->pixelToCoord(keyAxis->coordToPixel(keyAxis->range().lower)-strokeMargin*((keyAxis->orientation()==Qt::Vertical)!=keyAxis->rangeReversed()?-1:1)); + double rectRight = keyAxis->pixelToCoord(keyAxis->coordToPixel(keyAxis->range().upper)+strokeMargin*((keyAxis->orientation()==Qt::Vertical)!=keyAxis->rangeReversed()?-1:1)); + double rectBottom = valueAxis->pixelToCoord(valueAxis->coordToPixel(valueAxis->range().lower)+strokeMargin*((valueAxis->orientation()==Qt::Horizontal)!=valueAxis->rangeReversed()?-1:1)); + double rectTop = valueAxis->pixelToCoord(valueAxis->coordToPixel(valueAxis->range().upper)-strokeMargin*((valueAxis->orientation()==Qt::Horizontal)!=valueAxis->rangeReversed()?-1:1)); + int currentRegion; + QCPCurveDataMap::const_iterator it = mData->constBegin(); + QCPCurveDataMap::const_iterator prevIt = mData->constEnd()-1; + int prevRegion = getRegion(prevIt.value().key, prevIt.value().value, rectLeft, rectTop, rectRight, rectBottom); + QVector trailingPoints; // points that must be applied after all other points (are generated only when handling first point to get virtual segment between last and first point right) + while (it != mData->constEnd()) + { + currentRegion = getRegion(it.value().key, it.value().value, rectLeft, rectTop, rectRight, rectBottom); + if (currentRegion != prevRegion) // changed region, possibly need to add some optimized edge points or original points if entering R + { + if (currentRegion != 5) // segment doesn't end in R, so it's a candidate for removal + { + QPointF crossA, crossB; + if (prevRegion == 5) // we're coming from R, so add this point optimized + { + lineData->append(getOptimizedPoint(currentRegion, it.value().key, it.value().value, prevIt.value().key, prevIt.value().value, rectLeft, rectTop, rectRight, rectBottom)); + // in the situations 5->1/7/9/3 the segment may leave R and directly cross through two outer regions. In these cases we need to add an additional corner point + *lineData << getOptimizedCornerPoints(prevRegion, currentRegion, prevIt.value().key, prevIt.value().value, it.value().key, it.value().value, rectLeft, rectTop, rectRight, rectBottom); + } else if (mayTraverse(prevRegion, currentRegion) && + getTraverse(prevIt.value().key, prevIt.value().value, it.value().key, it.value().value, rectLeft, rectTop, rectRight, rectBottom, crossA, crossB)) + { + // add the two cross points optimized if segment crosses R and if segment isn't virtual zeroth segment between last and first curve point: + QVector beforeTraverseCornerPoints, afterTraverseCornerPoints; + getTraverseCornerPoints(prevRegion, currentRegion, rectLeft, rectTop, rectRight, rectBottom, beforeTraverseCornerPoints, afterTraverseCornerPoints); + if (it != mData->constBegin()) + { + *lineData << beforeTraverseCornerPoints; + lineData->append(crossA); + lineData->append(crossB); + *lineData << afterTraverseCornerPoints; + } else + { + lineData->append(crossB); + *lineData << afterTraverseCornerPoints; + trailingPoints << beforeTraverseCornerPoints << crossA ; + } + } else // doesn't cross R, line is just moving around in outside regions, so only need to add optimized point(s) at the boundary corner(s) + { + *lineData << getOptimizedCornerPoints(prevRegion, currentRegion, prevIt.value().key, prevIt.value().value, it.value().key, it.value().value, rectLeft, rectTop, rectRight, rectBottom); + } + } else // segment does end in R, so we add previous point optimized and this point at original position + { + if (it == mData->constBegin()) // it is first point in curve and prevIt is last one. So save optimized point for adding it to the lineData in the end + trailingPoints << getOptimizedPoint(prevRegion, prevIt.value().key, prevIt.value().value, it.value().key, it.value().value, rectLeft, rectTop, rectRight, rectBottom); + else + lineData->append(getOptimizedPoint(prevRegion, prevIt.value().key, prevIt.value().value, it.value().key, it.value().value, rectLeft, rectTop, rectRight, rectBottom)); + lineData->append(coordsToPixels(it.value().key, it.value().value)); + } + } else // region didn't change + { + if (currentRegion == 5) // still in R, keep adding original points + { + lineData->append(coordsToPixels(it.value().key, it.value().value)); + } else // still outside R, no need to add anything + { + // see how this is not doing anything? That's the main optimization... + } + } + prevIt = it; + prevRegion = currentRegion; + ++it; + } + *lineData << trailingPoints; +} + +/*! \internal + + This function is part of the curve optimization algorithm of \ref getCurveData. + + It returns the region of the given point (\a x, \a y) with respect to a rectangle defined by \a + rectLeft, \a rectTop, \a rectRight, and \a rectBottom. + + The regions are enumerated from top to bottom and left to right: + + + + + +
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+ + With the rectangle being region 5, and the outer regions extending infinitely outwards. In the + curve optimization algorithm, region 5 is considered to be the visible portion of the plot. +*/ +int QCPCurve::getRegion(double x, double y, double rectLeft, double rectTop, double rectRight, double rectBottom) const +{ + if (x < rectLeft) // region 123 + { + if (y > rectTop) + return 1; + else if (y < rectBottom) + return 3; + else + return 2; + } else if (x > rectRight) // region 789 + { + if (y > rectTop) + return 7; + else if (y < rectBottom) + return 9; + else + return 8; + } else // region 456 + { + if (y > rectTop) + return 4; + else if (y < rectBottom) + return 6; + else + return 5; + } +} + +/*! \internal + + This function is part of the curve optimization algorithm of \ref getCurveData. + + This method is used in case the current segment passes from inside the visible rect (region 5, + see \ref getRegion) to any of the outer regions (\a otherRegion). The current segment is given by + the line connecting (\a key, \a value) with (\a otherKey, \a otherValue). + + It returns the intersection point of the segment with the border of region 5. + + For this function it doesn't matter whether (\a key, \a value) is the point inside region 5 or + whether it's (\a otherKey, \a otherValue), i.e. whether the segment is coming from region 5 or + leaving it. It is important though that \a otherRegion correctly identifies the other region not + equal to 5. +*/ +QPointF QCPCurve::getOptimizedPoint(int otherRegion, double otherKey, double otherValue, double key, double value, double rectLeft, double rectTop, double rectRight, double rectBottom) const +{ + double intersectKey = rectLeft; // initial value is just fail-safe + double intersectValue = rectTop; // initial value is just fail-safe + switch (otherRegion) + { + case 1: // top and left edge + { + intersectValue = rectTop; + intersectKey = otherKey + (key-otherKey)/(value-otherValue)*(intersectValue-otherValue); + if (intersectKey < rectLeft || intersectKey > rectRight) // doesn't intersect, so must intersect other: + { + intersectKey = rectLeft; + intersectValue = otherValue + (value-otherValue)/(key-otherKey)*(intersectKey-otherKey); + } + break; + } + case 2: // left edge + { + intersectKey = rectLeft; + intersectValue = otherValue + (value-otherValue)/(key-otherKey)*(intersectKey-otherKey); + break; + } + case 3: // bottom and left edge + { + intersectValue = rectBottom; + intersectKey = otherKey + (key-otherKey)/(value-otherValue)*(intersectValue-otherValue); + if (intersectKey < rectLeft || intersectKey > rectRight) // doesn't intersect, so must intersect other: + { + intersectKey = rectLeft; + intersectValue = otherValue + (value-otherValue)/(key-otherKey)*(intersectKey-otherKey); + } + break; + } + case 4: // top edge + { + intersectValue = rectTop; + intersectKey = otherKey + (key-otherKey)/(value-otherValue)*(intersectValue-otherValue); + break; + } + case 5: + { + break; // case 5 shouldn't happen for this function but we add it anyway to prevent potential discontinuity in branch table + } + case 6: // bottom edge + { + intersectValue = rectBottom; + intersectKey = otherKey + (key-otherKey)/(value-otherValue)*(intersectValue-otherValue); + break; + } + case 7: // top and right edge + { + intersectValue = rectTop; + intersectKey = otherKey + (key-otherKey)/(value-otherValue)*(intersectValue-otherValue); + if (intersectKey < rectLeft || intersectKey > rectRight) // doesn't intersect, so must intersect other: + { + intersectKey = rectRight; + intersectValue = otherValue + (value-otherValue)/(key-otherKey)*(intersectKey-otherKey); + } + break; + } + case 8: // right edge + { + intersectKey = rectRight; + intersectValue = otherValue + (value-otherValue)/(key-otherKey)*(intersectKey-otherKey); + break; + } + case 9: // bottom and right edge + { + intersectValue = rectBottom; + intersectKey = otherKey + (key-otherKey)/(value-otherValue)*(intersectValue-otherValue); + if (intersectKey < rectLeft || intersectKey > rectRight) // doesn't intersect, so must intersect other: + { + intersectKey = rectRight; + intersectValue = otherValue + (value-otherValue)/(key-otherKey)*(intersectKey-otherKey); + } + break; + } + } + return coordsToPixels(intersectKey, intersectValue); +} + +/*! \internal + + This function is part of the curve optimization algorithm of \ref getCurveData. + + In situations where a single segment skips over multiple regions it might become necessary to add + extra points at the corners of region 5 (see \ref getRegion) such that the optimized segment + doesn't unintentionally cut through the visible area of the axis rect and create plot artifacts. + This method provides these points that must be added, assuming the original segment doesn't + start, end, or traverse region 5. (Corner points where region 5 is traversed are calculated by + \ref getTraverseCornerPoints.) + + For example, consider a segment which directly goes from region 4 to 2 but originally is far out + to the top left such that it doesn't cross region 5. Naively optimizing these points by + projecting them on the top and left borders of region 5 will create a segment that surely crosses + 5, creating a visual artifact in the plot. This method prevents this by providing extra points at + the top left corner, making the optimized curve correctly pass from region 4 to 1 to 2 without + traversing 5. +*/ +QVector QCPCurve::getOptimizedCornerPoints(int prevRegion, int currentRegion, double prevKey, double prevValue, double key, double value, double rectLeft, double rectTop, double rectRight, double rectBottom) const +{ + QVector result; + switch (prevRegion) + { + case 1: + { + switch (currentRegion) + { + case 2: { result << coordsToPixels(rectLeft, rectTop); break; } + case 4: { result << coordsToPixels(rectLeft, rectTop); break; } + case 3: { result << coordsToPixels(rectLeft, rectTop) << coordsToPixels(rectLeft, rectBottom); break; } + case 7: { result << coordsToPixels(rectLeft, rectTop) << coordsToPixels(rectRight, rectTop); break; } + case 6: { result << coordsToPixels(rectLeft, rectTop) << coordsToPixels(rectLeft, rectBottom); result.append(result.last()); break; } + case 8: { result << coordsToPixels(rectLeft, rectTop) << coordsToPixels(rectRight, rectTop); result.append(result.last()); break; } + case 9: { // in this case we need another distinction of cases: segment may pass below or above rect, requiring either bottom right or top left corner points + if ((value-prevValue)/(key-prevKey)*(rectLeft-key)+value < rectBottom) // segment passes below R + { result << coordsToPixels(rectLeft, rectTop) << coordsToPixels(rectLeft, rectBottom); result.append(result.last()); result << coordsToPixels(rectRight, rectBottom); } + else + { result << coordsToPixels(rectLeft, rectTop) << coordsToPixels(rectRight, rectTop); result.append(result.last()); result << coordsToPixels(rectRight, rectBottom); } + break; + } + } + break; + } + case 2: + { + switch (currentRegion) + { + case 1: { result << coordsToPixels(rectLeft, rectTop); break; } + case 3: { result << coordsToPixels(rectLeft, rectBottom); break; } + case 4: { result << coordsToPixels(rectLeft, rectTop); result.append(result.last()); break; } + case 6: { result << coordsToPixels(rectLeft, rectBottom); result.append(result.last()); break; } + case 7: { result << coordsToPixels(rectLeft, rectTop); result.append(result.last()); result << coordsToPixels(rectRight, rectTop); break; } + case 9: { result << coordsToPixels(rectLeft, rectBottom); result.append(result.last()); result << coordsToPixels(rectRight, rectBottom); break; } + } + break; + } + case 3: + { + switch (currentRegion) + { + case 2: { result << coordsToPixels(rectLeft, rectBottom); break; } + case 6: { result << coordsToPixels(rectLeft, rectBottom); break; } + case 1: { result << coordsToPixels(rectLeft, rectBottom) << coordsToPixels(rectLeft, rectTop); break; } + case 9: { result << coordsToPixels(rectLeft, rectBottom) << coordsToPixels(rectRight, rectBottom); break; } + case 4: { result << coordsToPixels(rectLeft, rectBottom) << coordsToPixels(rectLeft, rectTop); result.append(result.last()); break; } + case 8: { result << coordsToPixels(rectLeft, rectBottom) << coordsToPixels(rectRight, rectBottom); result.append(result.last()); break; } + case 7: { // in this case we need another distinction of cases: segment may pass below or above rect, requiring either bottom right or top left corner points + if ((value-prevValue)/(key-prevKey)*(rectRight-key)+value < rectBottom) // segment passes below R + { result << coordsToPixels(rectLeft, rectBottom) << coordsToPixels(rectRight, rectBottom); result.append(result.last()); result << coordsToPixels(rectRight, rectTop); } + else + { result << coordsToPixels(rectLeft, rectBottom) << coordsToPixels(rectLeft, rectTop); result.append(result.last()); result << coordsToPixels(rectRight, rectTop); } + break; + } + } + break; + } + case 4: + { + switch (currentRegion) + { + case 1: { result << coordsToPixels(rectLeft, rectTop); break; } + case 7: { result << coordsToPixels(rectRight, rectTop); break; } + case 2: { result << coordsToPixels(rectLeft, rectTop); result.append(result.last()); break; } + case 8: { result << coordsToPixels(rectRight, rectTop); result.append(result.last()); break; } + case 3: { result << coordsToPixels(rectLeft, rectTop); result.append(result.last()); result << coordsToPixels(rectLeft, rectBottom); break; } + case 9: { result << coordsToPixels(rectRight, rectTop); result.append(result.last()); result << coordsToPixels(rectRight, rectBottom); break; } + } + break; + } + case 5: + { + switch (currentRegion) + { + case 1: { result << coordsToPixels(rectLeft, rectTop); break; } + case 7: { result << coordsToPixels(rectRight, rectTop); break; } + case 9: { result << coordsToPixels(rectRight, rectBottom); break; } + case 3: { result << coordsToPixels(rectLeft, rectBottom); break; } + } + break; + } + case 6: + { + switch (currentRegion) + { + case 3: { result << coordsToPixels(rectLeft, rectBottom); break; } + case 9: { result << coordsToPixels(rectRight, rectBottom); break; } + case 2: { result << coordsToPixels(rectLeft, rectBottom); result.append(result.last()); break; } + case 8: { result << coordsToPixels(rectRight, rectBottom); result.append(result.last()); break; } + case 1: { result << coordsToPixels(rectLeft, rectBottom); result.append(result.last()); result << coordsToPixels(rectLeft, rectTop); break; } + case 7: { result << coordsToPixels(rectRight, rectBottom); result.append(result.last()); result << coordsToPixels(rectRight, rectTop); break; } + } + break; + } + case 7: + { + switch (currentRegion) + { + case 4: { result << coordsToPixels(rectRight, rectTop); break; } + case 8: { result << coordsToPixels(rectRight, rectTop); break; } + case 1: { result << coordsToPixels(rectRight, rectTop) << coordsToPixels(rectLeft, rectTop); break; } + case 9: { result << coordsToPixels(rectRight, rectTop) << coordsToPixels(rectRight, rectBottom); break; } + case 2: { result << coordsToPixels(rectRight, rectTop) << coordsToPixels(rectLeft, rectTop); result.append(result.last()); break; } + case 6: { result << coordsToPixels(rectRight, rectTop) << coordsToPixels(rectRight, rectBottom); result.append(result.last()); break; } + case 3: { // in this case we need another distinction of cases: segment may pass below or above rect, requiring either bottom right or top left corner points + if ((value-prevValue)/(key-prevKey)*(rectRight-key)+value < rectBottom) // segment passes below R + { result << coordsToPixels(rectRight, rectTop) << coordsToPixels(rectRight, rectBottom); result.append(result.last()); result << coordsToPixels(rectLeft, rectBottom); } + else + { result << coordsToPixels(rectRight, rectTop) << coordsToPixels(rectLeft, rectTop); result.append(result.last()); result << coordsToPixels(rectLeft, rectBottom); } + break; + } + } + break; + } + case 8: + { + switch (currentRegion) + { + case 7: { result << coordsToPixels(rectRight, rectTop); break; } + case 9: { result << coordsToPixels(rectRight, rectBottom); break; } + case 4: { result << coordsToPixels(rectRight, rectTop); result.append(result.last()); break; } + case 6: { result << coordsToPixels(rectRight, rectBottom); result.append(result.last()); break; } + case 1: { result << coordsToPixels(rectRight, rectTop); result.append(result.last()); result << coordsToPixels(rectLeft, rectTop); break; } + case 3: { result << coordsToPixels(rectRight, rectBottom); result.append(result.last()); result << coordsToPixels(rectLeft, rectBottom); break; } + } + break; + } + case 9: + { + switch (currentRegion) + { + case 6: { result << coordsToPixels(rectRight, rectBottom); break; } + case 8: { result << coordsToPixels(rectRight, rectBottom); break; } + case 3: { result << coordsToPixels(rectRight, rectBottom) << coordsToPixels(rectLeft, rectBottom); break; } + case 7: { result << coordsToPixels(rectRight, rectBottom) << coordsToPixels(rectRight, rectTop); break; } + case 2: { result << coordsToPixels(rectRight, rectBottom) << coordsToPixels(rectLeft, rectBottom); result.append(result.last()); break; } + case 4: { result << coordsToPixels(rectRight, rectBottom) << coordsToPixels(rectRight, rectTop); result.append(result.last()); break; } + case 1: { // in this case we need another distinction of cases: segment may pass below or above rect, requiring either bottom right or top left corner points + if ((value-prevValue)/(key-prevKey)*(rectLeft-key)+value < rectBottom) // segment passes below R + { result << coordsToPixels(rectRight, rectBottom) << coordsToPixels(rectLeft, rectBottom); result.append(result.last()); result << coordsToPixels(rectLeft, rectTop); } + else + { result << coordsToPixels(rectRight, rectBottom) << coordsToPixels(rectRight, rectTop); result.append(result.last()); result << coordsToPixels(rectLeft, rectTop); } + break; + } + } + break; + } + } + return result; +} + +/*! \internal + + This function is part of the curve optimization algorithm of \ref getCurveData. + + This method returns whether a segment going from \a prevRegion to \a currentRegion (see \ref + getRegion) may traverse the visible region 5. This function assumes that neither \a prevRegion + nor \a currentRegion is 5 itself. + + If this method returns false, the segment for sure doesn't pass region 5. If it returns true, the + segment may or may not pass region 5 and a more fine-grained calculation must be used (\ref + getTraverse). +*/ +bool QCPCurve::mayTraverse(int prevRegion, int currentRegion) const +{ + switch (prevRegion) + { + case 1: + { + switch (currentRegion) + { + case 4: + case 7: + case 2: + case 3: return false; + default: return true; + } + } + case 2: + { + switch (currentRegion) + { + case 1: + case 3: return false; + default: return true; + } + } + case 3: + { + switch (currentRegion) + { + case 1: + case 2: + case 6: + case 9: return false; + default: return true; + } + } + case 4: + { + switch (currentRegion) + { + case 1: + case 7: return false; + default: return true; + } + } + case 5: return false; // should never occur + case 6: + { + switch (currentRegion) + { + case 3: + case 9: return false; + default: return true; + } + } + case 7: + { + switch (currentRegion) + { + case 1: + case 4: + case 8: + case 9: return false; + default: return true; + } + } + case 8: + { + switch (currentRegion) + { + case 7: + case 9: return false; + default: return true; + } + } + case 9: + { + switch (currentRegion) + { + case 3: + case 6: + case 8: + case 7: return false; + default: return true; + } + } + default: return true; + } +} + + +/*! \internal + + This function is part of the curve optimization algorithm of \ref getCurveData. + + This method assumes that the \ref mayTraverse test has returned true, so there is a chance the + segment defined by (\a prevKey, \a prevValue) and (\a key, \a value) goes through the visible + region 5. + + The return value of this method indicates whether the segment actually traverses region 5 or not. + + If the segment traverses 5, the output parameters \a crossA and \a crossB indicate the entry and + exit points of region 5. They will become the optimized points for that segment. +*/ +bool QCPCurve::getTraverse(double prevKey, double prevValue, double key, double value, double rectLeft, double rectTop, double rectRight, double rectBottom, QPointF &crossA, QPointF &crossB) const +{ + QList intersections; // x of QPointF corresponds to key and y to value + if (qFuzzyIsNull(key-prevKey)) // line is parallel to value axis + { + // due to region filter in mayTraverseR(), if line is parallel to value or key axis, R is traversed here + intersections.append(QPointF(key, rectBottom)); // direction will be taken care of at end of method + intersections.append(QPointF(key, rectTop)); + } else if (qFuzzyIsNull(value-prevValue)) // line is parallel to key axis + { + // due to region filter in mayTraverseR(), if line is parallel to value or key axis, R is traversed here + intersections.append(QPointF(rectLeft, value)); // direction will be taken care of at end of method + intersections.append(QPointF(rectRight, value)); + } else // line is skewed + { + double gamma; + double keyPerValue = (key-prevKey)/(value-prevValue); + // check top of rect: + gamma = prevKey + (rectTop-prevValue)*keyPerValue; + if (gamma >= rectLeft && gamma <= rectRight) + intersections.append(QPointF(gamma, rectTop)); + // check bottom of rect: + gamma = prevKey + (rectBottom-prevValue)*keyPerValue; + if (gamma >= rectLeft && gamma <= rectRight) + intersections.append(QPointF(gamma, rectBottom)); + double valuePerKey = 1.0/keyPerValue; + // check left of rect: + gamma = prevValue + (rectLeft-prevKey)*valuePerKey; + if (gamma >= rectBottom && gamma <= rectTop) + intersections.append(QPointF(rectLeft, gamma)); + // check right of rect: + gamma = prevValue + (rectRight-prevKey)*valuePerKey; + if (gamma >= rectBottom && gamma <= rectTop) + intersections.append(QPointF(rectRight, gamma)); + } + + // handle cases where found points isn't exactly 2: + if (intersections.size() > 2) + { + // line probably goes through corner of rect, and we got duplicate points there. single out the point pair with greatest distance in between: + double distSqrMax = 0; + QPointF pv1, pv2; + for (int i=0; i distSqrMax) + { + pv1 = intersections.at(i); + pv2 = intersections.at(k); + distSqrMax = distSqr; + } + } + } + intersections = QList() << pv1 << pv2; + } else if (intersections.size() != 2) + { + // one or even zero points found (shouldn't happen unless line perfectly tangent to corner), no need to draw segment + return false; + } + + // possibly re-sort points so optimized point segment has same direction as original segment: + if ((key-prevKey)*(intersections.at(1).x()-intersections.at(0).x()) + (value-prevValue)*(intersections.at(1).y()-intersections.at(0).y()) < 0) // scalar product of both segments < 0 -> opposite direction + intersections.move(0, 1); + crossA = coordsToPixels(intersections.at(0).x(), intersections.at(0).y()); + crossB = coordsToPixels(intersections.at(1).x(), intersections.at(1).y()); + return true; +} + +/*! \internal + + This function is part of the curve optimization algorithm of \ref getCurveData. + + This method assumes that the \ref getTraverse test has returned true, so the segment definitely + traverses the visible region 5 when going from \a prevRegion to \a currentRegion. + + In certain situations it is not sufficient to merely generate the entry and exit points of the + segment into/out of region 5, as \ref getTraverse provides. It may happen that a single segment, in + addition to traversing region 5, skips another region outside of region 5, which makes it + necessary to add an optimized corner point there (very similar to the job \ref + getOptimizedCornerPoints does for segments that are completely in outside regions and don't + traverse 5). + + As an example, consider a segment going from region 1 to region 6, traversing the lower left + corner of region 5. In this configuration, the segment additionally crosses the border between + region 1 and 2 before entering region 5. This makes it necessary to add an additional point in + the top left corner, before adding the optimized traverse points. So in this case, the output + parameter \a beforeTraverse will contain the top left corner point, and \a afterTraverse will be + empty. + + In some cases, such as when going from region 1 to 9, it may even be necessary to add additional + corner points before and after the traverse. Then both \a beforeTraverse and \a afterTraverse + return the respective corner points. +*/ +void QCPCurve::getTraverseCornerPoints(int prevRegion, int currentRegion, double rectLeft, double rectTop, double rectRight, double rectBottom, QVector &beforeTraverse, QVector &afterTraverse) const +{ + switch (prevRegion) + { + case 1: + { + switch (currentRegion) + { + case 6: { beforeTraverse << coordsToPixels(rectLeft, rectTop); break; } + case 9: { beforeTraverse << coordsToPixels(rectLeft, rectTop); afterTraverse << coordsToPixels(rectRight, rectBottom); break; } + case 8: { beforeTraverse << coordsToPixels(rectLeft, rectTop); break; } + } + break; + } + case 2: + { + switch (currentRegion) + { + case 7: { afterTraverse << coordsToPixels(rectRight, rectTop); break; } + case 9: { afterTraverse << coordsToPixels(rectRight, rectBottom); break; } + } + break; + } + case 3: + { + switch (currentRegion) + { + case 4: { beforeTraverse << coordsToPixels(rectLeft, rectBottom); break; } + case 7: { beforeTraverse << coordsToPixels(rectLeft, rectBottom); afterTraverse << coordsToPixels(rectRight, rectTop); break; } + case 8: { beforeTraverse << coordsToPixels(rectLeft, rectBottom); break; } + } + break; + } + case 4: + { + switch (currentRegion) + { + case 3: { afterTraverse << coordsToPixels(rectLeft, rectBottom); break; } + case 9: { afterTraverse << coordsToPixels(rectRight, rectBottom); break; } + } + break; + } + case 5: { break; } // shouldn't happen because this method only handles full traverses + case 6: + { + switch (currentRegion) + { + case 1: { afterTraverse << coordsToPixels(rectLeft, rectTop); break; } + case 7: { afterTraverse << coordsToPixels(rectRight, rectTop); break; } + } + break; + } + case 7: + { + switch (currentRegion) + { + case 2: { beforeTraverse << coordsToPixels(rectRight, rectTop); break; } + case 3: { beforeTraverse << coordsToPixels(rectRight, rectTop); afterTraverse << coordsToPixels(rectLeft, rectBottom); break; } + case 6: { beforeTraverse << coordsToPixels(rectRight, rectTop); break; } + } + break; + } + case 8: + { + switch (currentRegion) + { + case 1: { afterTraverse << coordsToPixels(rectLeft, rectTop); break; } + case 3: { afterTraverse << coordsToPixels(rectLeft, rectBottom); break; } + } + break; + } + case 9: + { + switch (currentRegion) + { + case 2: { beforeTraverse << coordsToPixels(rectRight, rectBottom); break; } + case 1: { beforeTraverse << coordsToPixels(rectRight, rectBottom); afterTraverse << coordsToPixels(rectLeft, rectTop); break; } + case 4: { beforeTraverse << coordsToPixels(rectRight, rectBottom); break; } + } + break; + } + } +} + +/*! \internal + + Calculates the (minimum) distance (in pixels) the curve's representation has from the given \a + pixelPoint in pixels. This is used to determine whether the curve was clicked or not, e.g. in + \ref selectTest. +*/ +double QCPCurve::pointDistance(const QPointF &pixelPoint) const +{ + if (mData->isEmpty()) + { + qDebug() << Q_FUNC_INFO << "requested point distance on curve" << mName << "without data"; + return 500; + } + if (mData->size() == 1) + { + QPointF dataPoint = coordsToPixels(mData->constBegin().key(), mData->constBegin().value().value); + return QVector2D(dataPoint-pixelPoint).length(); + } + + // calculate minimum distance to line segments: + QVector *lineData = new QVector; + getCurveData(lineData); + double minDistSqr = std::numeric_limits::max(); + for (int i=0; isize()-1; ++i) + { + double currentDistSqr = distSqrToLine(lineData->at(i), lineData->at(i+1), pixelPoint); + if (currentDistSqr < minDistSqr) + minDistSqr = currentDistSqr; + } + delete lineData; + return qSqrt(minDistSqr); +} + +/* inherits documentation from base class */ +QCPRange QCPCurve::getKeyRange(bool &foundRange, SignDomain inSignDomain) const +{ + QCPRange range; + bool haveLower = false; + bool haveUpper = false; + + double current; + + QCPCurveDataMap::const_iterator it = mData->constBegin(); + while (it != mData->constEnd()) + { + current = it.value().key; + if (!qIsNaN(current) && !qIsNaN(it.value().value)) + { + if (inSignDomain == sdBoth || (inSignDomain == sdNegative && current < 0) || (inSignDomain == sdPositive && current > 0)) + { + if (current < range.lower || !haveLower) + { + range.lower = current; + haveLower = true; + } + if (current > range.upper || !haveUpper) + { + range.upper = current; + haveUpper = true; + } + } + } + ++it; + } + + foundRange = haveLower && haveUpper; + return range; +} + +/* inherits documentation from base class */ +QCPRange QCPCurve::getValueRange(bool &foundRange, SignDomain inSignDomain) const +{ + QCPRange range; + bool haveLower = false; + bool haveUpper = false; + + double current; + + QCPCurveDataMap::const_iterator it = mData->constBegin(); + while (it != mData->constEnd()) + { + current = it.value().value; + if (!qIsNaN(current) && !qIsNaN(it.value().key)) + { + if (inSignDomain == sdBoth || (inSignDomain == sdNegative && current < 0) || (inSignDomain == sdPositive && current > 0)) + { + if (current < range.lower || !haveLower) + { + range.lower = current; + haveLower = true; + } + if (current > range.upper || !haveUpper) + { + range.upper = current; + haveUpper = true; + } + } + } + ++it; + } + + foundRange = haveLower && haveUpper; + return range; +} + + +//////////////////////////////////////////////////////////////////////////////////////////////////// +//////////////////// QCPBarsGroup +//////////////////////////////////////////////////////////////////////////////////////////////////// + +/*! \class QCPBarsGroup + \brief Groups multiple QCPBars together so they appear side by side + + \image html QCPBarsGroup.png + + When showing multiple QCPBars in one plot which have bars at identical keys, it may be desirable + to have them appearing next to each other at each key. This is what adding the respective QCPBars + plottables to a QCPBarsGroup achieves. (An alternative approach is to stack them on top of each + other, see \ref QCPBars::moveAbove.) + + \section qcpbarsgroup-usage Usage + + To add a QCPBars plottable to the group, create a new group and then add the respective bars + intances: + \snippet documentation/doc-code-snippets/mainwindow.cpp qcpbarsgroup-creation + Alternatively to appending to the group like shown above, you can also set the group on the + QCPBars plottable via \ref QCPBars::setBarsGroup. + + The spacing between the bars can be configured via \ref setSpacingType and \ref setSpacing. The + bars in this group appear in the plot in the order they were appended. To insert a bars plottable + at a certain index position, or to reposition a bars plottable which is already in the group, use + \ref insert. + + To remove specific bars from the group, use either \ref remove or call \ref + QCPBars::setBarsGroup "QCPBars::setBarsGroup(0)" on the respective bars plottable. + + To clear the entire group, call \ref clear, or simply delete the group. + + \section qcpbarsgroup-example Example + + The image above is generated with the following code: + \snippet documentation/doc-image-generator/mainwindow.cpp qcpbarsgroup-example +*/ + +/* start of documentation of inline functions */ + +/*! \fn QList QCPBarsGroup::bars() const + + Returns all bars currently in this group. + + \see bars(int index) +*/ + +/*! \fn int QCPBarsGroup::size() const + + Returns the number of QCPBars plottables that are part of this group. + +*/ + +/*! \fn bool QCPBarsGroup::isEmpty() const + + Returns whether this bars group is empty. + + \see size +*/ + +/*! \fn bool QCPBarsGroup::contains(QCPBars *bars) + + Returns whether the specified \a bars plottable is part of this group. + +*/ + +/* end of documentation of inline functions */ + +/*! + Constructs a new bars group for the specified QCustomPlot instance. +*/ +QCPBarsGroup::QCPBarsGroup(QCustomPlot *parentPlot) : + QObject(parentPlot), + mParentPlot(parentPlot), + mSpacingType(stAbsolute), + mSpacing(4) +{ +} + +QCPBarsGroup::~QCPBarsGroup() +{ + clear(); +} + +/*! + Sets how the spacing between adjacent bars is interpreted. See \ref SpacingType. + + The actual spacing can then be specified with \ref setSpacing. + + \see setSpacing +*/ +void QCPBarsGroup::setSpacingType(SpacingType spacingType) +{ + mSpacingType = spacingType; +} + +/*! + Sets the spacing between adjacent bars. What the number passed as \a spacing actually means, is + defined by the current \ref SpacingType, which can be set with \ref setSpacingType. + + \see setSpacingType +*/ +void QCPBarsGroup::setSpacing(double spacing) +{ + mSpacing = spacing; +} + +/*! + Returns the QCPBars instance with the specified \a index in this group. If no such QCPBars + exists, returns 0. + + \see bars(), size +*/ +QCPBars *QCPBarsGroup::bars(int index) const +{ + if (index >= 0 && index < mBars.size()) + { + return mBars.at(index); + } else + { + qDebug() << Q_FUNC_INFO << "index out of bounds:" << index; + return 0; + } +} + +/*! + Removes all QCPBars plottables from this group. + + \see isEmpty +*/ +void QCPBarsGroup::clear() +{ + foreach (QCPBars *bars, mBars) // since foreach takes a copy, removing bars in the loop is okay + bars->setBarsGroup(0); // removes itself via removeBars +} + +/*! + Adds the specified \a bars plottable to this group. Alternatively, you can also use \ref + QCPBars::setBarsGroup on the \a bars instance. + + \see insert, remove +*/ +void QCPBarsGroup::append(QCPBars *bars) +{ + if (!bars) + { + qDebug() << Q_FUNC_INFO << "bars is 0"; + return; + } + + if (!mBars.contains(bars)) + bars->setBarsGroup(this); + else + qDebug() << Q_FUNC_INFO << "bars plottable is already in this bars group:" << reinterpret_cast(bars); +} + +/*! + Inserts the specified \a bars plottable into this group at the specified index position \a i. + This gives you full control over the ordering of the bars. + + \a bars may already be part of this group. In that case, \a bars is just moved to the new index + position. + + \see append, remove +*/ +void QCPBarsGroup::insert(int i, QCPBars *bars) +{ + if (!bars) + { + qDebug() << Q_FUNC_INFO << "bars is 0"; + return; + } + + // first append to bars list normally: + if (!mBars.contains(bars)) + bars->setBarsGroup(this); + // then move to according position: + mBars.move(mBars.indexOf(bars), qBound(0, i, mBars.size()-1)); +} + +/*! + Removes the specified \a bars plottable from this group. + + \see contains, clear +*/ +void QCPBarsGroup::remove(QCPBars *bars) +{ + if (!bars) + { + qDebug() << Q_FUNC_INFO << "bars is 0"; + return; + } + + if (mBars.contains(bars)) + bars->setBarsGroup(0); + else + qDebug() << Q_FUNC_INFO << "bars plottable is not in this bars group:" << reinterpret_cast(bars); +} + +/*! \internal + + Adds the specified \a bars to the internal mBars list of bars. This method does not change the + barsGroup property on \a bars. + + \see unregisterBars +*/ +void QCPBarsGroup::registerBars(QCPBars *bars) +{ + if (!mBars.contains(bars)) + mBars.append(bars); +} + +/*! \internal + + Removes the specified \a bars from the internal mBars list of bars. This method does not change + the barsGroup property on \a bars. + + \see registerBars +*/ +void QCPBarsGroup::unregisterBars(QCPBars *bars) +{ + mBars.removeOne(bars); +} + +/*! \internal + + Returns the pixel offset in the key dimension the specified \a bars plottable should have at the + given key coordinate \a keyCoord. The offset is relative to the pixel position of the key + coordinate \a keyCoord. +*/ +double QCPBarsGroup::keyPixelOffset(const QCPBars *bars, double keyCoord) +{ + // find list of all base bars in case some mBars are stacked: + QList baseBars; + foreach (const QCPBars *b, mBars) + { + while (b->barBelow()) + b = b->barBelow(); + if (!baseBars.contains(b)) + baseBars.append(b); + } + // find base bar this "bars" is stacked on: + const QCPBars *thisBase = bars; + while (thisBase->barBelow()) + thisBase = thisBase->barBelow(); + + // determine key pixel offset of this base bars considering all other base bars in this barsgroup: + double result = 0; + int index = baseBars.indexOf(thisBase); + if (index >= 0) + { + int startIndex; + double lowerPixelWidth, upperPixelWidth; + if (baseBars.size() % 2 == 1 && index == (baseBars.size()-1)/2) // is center bar (int division on purpose) + { + return result; + } else if (index < (baseBars.size()-1)/2.0) // bar is to the left of center + { + if (baseBars.size() % 2 == 0) // even number of bars + { + startIndex = baseBars.size()/2-1; + result -= getPixelSpacing(baseBars.at(startIndex), keyCoord)*0.5; // half of middle spacing + } else // uneven number of bars + { + startIndex = (baseBars.size()-1)/2-1; + baseBars.at((baseBars.size()-1)/2)->getPixelWidth(keyCoord, lowerPixelWidth, upperPixelWidth); + result -= qAbs(upperPixelWidth-lowerPixelWidth)*0.5; // half of center bar + result -= getPixelSpacing(baseBars.at((baseBars.size()-1)/2), keyCoord); // center bar spacing + } + for (int i=startIndex; i>index; --i) // add widths and spacings of bars in between center and our bars + { + baseBars.at(i)->getPixelWidth(keyCoord, lowerPixelWidth, upperPixelWidth); + result -= qAbs(upperPixelWidth-lowerPixelWidth); + result -= getPixelSpacing(baseBars.at(i), keyCoord); + } + // finally half of our bars width: + baseBars.at(index)->getPixelWidth(keyCoord, lowerPixelWidth, upperPixelWidth); + result -= qAbs(upperPixelWidth-lowerPixelWidth)*0.5; + } else // bar is to the right of center + { + if (baseBars.size() % 2 == 0) // even number of bars + { + startIndex = baseBars.size()/2; + result += getPixelSpacing(baseBars.at(startIndex), keyCoord)*0.5; // half of middle spacing + } else // uneven number of bars + { + startIndex = (baseBars.size()-1)/2+1; + baseBars.at((baseBars.size()-1)/2)->getPixelWidth(keyCoord, lowerPixelWidth, upperPixelWidth); + result += qAbs(upperPixelWidth-lowerPixelWidth)*0.5; // half of center bar + result += getPixelSpacing(baseBars.at((baseBars.size()-1)/2), keyCoord); // center bar spacing + } + for (int i=startIndex; igetPixelWidth(keyCoord, lowerPixelWidth, upperPixelWidth); + result += qAbs(upperPixelWidth-lowerPixelWidth); + result += getPixelSpacing(baseBars.at(i), keyCoord); + } + // finally half of our bars width: + baseBars.at(index)->getPixelWidth(keyCoord, lowerPixelWidth, upperPixelWidth); + result += qAbs(upperPixelWidth-lowerPixelWidth)*0.5; + } + } + return result; +} + +/*! \internal + + Returns the spacing in pixels which is between this \a bars and the following one, both at the + key coordinate \a keyCoord. + + \note Typically the returned value doesn't depend on \a bars or \a keyCoord. \a bars is only + needed to get acces to the key axis transformation and axis rect for the modes \ref + stAxisRectRatio and \ref stPlotCoords. The \a keyCoord is only relevant for spacings given in + \ref stPlotCoords on a logarithmic axis. +*/ +double QCPBarsGroup::getPixelSpacing(const QCPBars *bars, double keyCoord) +{ + switch (mSpacingType) + { + case stAbsolute: + { + return mSpacing; + } + case stAxisRectRatio: + { + if (bars->keyAxis()->orientation() == Qt::Horizontal) + return bars->keyAxis()->axisRect()->width()*mSpacing; + else + return bars->keyAxis()->axisRect()->height()*mSpacing; + } + case stPlotCoords: + { + double keyPixel = bars->keyAxis()->coordToPixel(keyCoord); + return bars->keyAxis()->coordToPixel(keyCoord+mSpacing)-keyPixel; + } + } + return 0; +} + + +//////////////////////////////////////////////////////////////////////////////////////////////////// +//////////////////// QCPBarData +//////////////////////////////////////////////////////////////////////////////////////////////////// + +/*! \class QCPBarData + \brief Holds the data of one single data point (one bar) for QCPBars. + + The container for storing multiple data points is \ref QCPBarDataMap. + + The stored data is: + \li \a key: coordinate on the key axis of this bar + \li \a value: height coordinate on the value axis of this bar + + \see QCPBarDataaMap +*/ + +/*! + Constructs a bar data point with key and value set to zero. +*/ +QCPBarData::QCPBarData() : + key(0), + value(0) +{ +} + +/*! + Constructs a bar data point with the specified \a key and \a value. +*/ +QCPBarData::QCPBarData(double key, double value) : + key(key), + value(value) +{ +} + + +//////////////////////////////////////////////////////////////////////////////////////////////////// +//////////////////// QCPBars +//////////////////////////////////////////////////////////////////////////////////////////////////// + +/*! \class QCPBars + \brief A plottable representing a bar chart in a plot. + + \image html QCPBars.png + + To plot data, assign it with the \ref setData or \ref addData functions. + + \section appearance Changing the appearance + + The appearance of the bars is determined by the pen and the brush (\ref setPen, \ref setBrush). + The width of the individual bars can be controlled with \ref setWidthType and \ref setWidth. + + Bar charts are stackable. This means, two QCPBars plottables can be placed on top of each other + (see \ref QCPBars::moveAbove). So when two bars are at the same key position, they will appear + stacked. + + If you would like to group multiple QCPBars plottables together so they appear side by side as + shown below, use QCPBarsGroup. + + \image html QCPBarsGroup.png + + \section usage Usage + + Like all data representing objects in QCustomPlot, the QCPBars is a plottable + (QCPAbstractPlottable). So the plottable-interface of QCustomPlot applies + (QCustomPlot::plottable, QCustomPlot::addPlottable, QCustomPlot::removePlottable, etc.) + + Usually, you first create an instance: + \snippet documentation/doc-code-snippets/mainwindow.cpp qcpbars-creation-1 + add it to the customPlot with QCustomPlot::addPlottable: + \snippet documentation/doc-code-snippets/mainwindow.cpp qcpbars-creation-2 + and then modify the properties of the newly created plottable, e.g.: + \snippet documentation/doc-code-snippets/mainwindow.cpp qcpbars-creation-3 +*/ + +/* start of documentation of inline functions */ + +/*! \fn QCPBars *QCPBars::barBelow() const + Returns the bars plottable that is directly below this bars plottable. + If there is no such plottable, returns 0. + + \see barAbove, moveBelow, moveAbove +*/ + +/*! \fn QCPBars *QCPBars::barAbove() const + Returns the bars plottable that is directly above this bars plottable. + If there is no such plottable, returns 0. + + \see barBelow, moveBelow, moveAbove +*/ + +/* end of documentation of inline functions */ + +/*! + Constructs a bar chart which uses \a keyAxis as its key axis ("x") and \a valueAxis as its value + axis ("y"). \a keyAxis and \a valueAxis must reside in the same QCustomPlot instance and not have + the same orientation. If either of these restrictions is violated, a corresponding message is + printed to the debug output (qDebug), the construction is not aborted, though. + + The constructed QCPBars can be added to the plot with QCustomPlot::addPlottable, QCustomPlot + then takes ownership of the bar chart. +*/ +QCPBars::QCPBars(QCPAxis *keyAxis, QCPAxis *valueAxis) : + QCPAbstractPlottable(keyAxis, valueAxis), + mData(new QCPBarDataMap), + mWidth(0.75), + mWidthType(wtPlotCoords), + mBarsGroup(0), + mBaseValue(0) +{ + // modify inherited properties from abstract plottable: + mPen.setColor(Qt::blue); + mPen.setStyle(Qt::SolidLine); + mBrush.setColor(QColor(40, 50, 255, 30)); + mBrush.setStyle(Qt::SolidPattern); + mSelectedPen = mPen; + mSelectedPen.setWidthF(2.5); + mSelectedPen.setColor(QColor(80, 80, 255)); // lighter than Qt::blue of mPen + mSelectedBrush = mBrush; +} + +QCPBars::~QCPBars() +{ + setBarsGroup(0); + if (mBarBelow || mBarAbove) + connectBars(mBarBelow.data(), mBarAbove.data()); // take this bar out of any stacking + delete mData; +} + +/*! + Sets the width of the bars. + + How the number passed as \a width is interpreted (e.g. screen pixels, plot coordinates,...), + depends on the currently set width type, see \ref setWidthType and \ref WidthType. +*/ +void QCPBars::setWidth(double width) +{ + mWidth = width; +} + +/*! + Sets how the width of the bars is defined. See the documentation of \ref WidthType for an + explanation of the possible values for \a widthType. + + The default value is \ref wtPlotCoords. + + \see setWidth +*/ +void QCPBars::setWidthType(QCPBars::WidthType widthType) +{ + mWidthType = widthType; +} + +/*! + Sets to which QCPBarsGroup this QCPBars instance belongs to. Alternatively, you can also use \ref + QCPBarsGroup::append. + + To remove this QCPBars from any group, set \a barsGroup to 0. +*/ +void QCPBars::setBarsGroup(QCPBarsGroup *barsGroup) +{ + // deregister at old group: + if (mBarsGroup) + mBarsGroup->unregisterBars(this); + mBarsGroup = barsGroup; + // register at new group: + if (mBarsGroup) + mBarsGroup->registerBars(this); +} + +/*! + Sets the base value of this bars plottable. + + The base value defines where on the value coordinate the bars start. How far the bars extend from + the base value is given by their individual value data. For example, if the base value is set to + 1, a bar with data value 2 will have its lowest point at value coordinate 1 and highest point at + 3. + + For stacked bars, only the base value of the bottom-most QCPBars has meaning. + + The default base value is 0. +*/ +void QCPBars::setBaseValue(double baseValue) +{ + mBaseValue = baseValue; +} + +/*! + Replaces the current data with the provided \a data. + + If \a copy is set to true, data points in \a data will only be copied. if false, the plottable + takes ownership of the passed data and replaces the internal data pointer with it. This is + significantly faster than copying for large datasets. +*/ +void QCPBars::setData(QCPBarDataMap *data, bool copy) +{ + if (mData == data) + { + qDebug() << Q_FUNC_INFO << "The data pointer is already in (and owned by) this plottable" << reinterpret_cast(data); + return; + } + if (copy) + { + *mData = *data; + } else + { + delete mData; + mData = data; + } +} + +/*! \overload + + Replaces the current data with the provided points in \a key and \a value tuples. The + provided vectors should have equal length. Else, the number of added points will be the size of + the smallest vector. +*/ +void QCPBars::setData(const QVector &key, const QVector &value) +{ + mData->clear(); + int n = key.size(); + n = qMin(n, value.size()); + QCPBarData newData; + for (int i=0; iinsertMulti(newData.key, newData); + } +} + +/*! + Moves this bars plottable below \a bars. In other words, the bars of this plottable will appear + below the bars of \a bars. The move target \a bars must use the same key and value axis as this + plottable. + + Inserting into and removing from existing bar stacking is handled gracefully. If \a bars already + has a bars object below itself, this bars object is inserted between the two. If this bars object + is already between two other bars, the two other bars will be stacked on top of each other after + the operation. + + To remove this bars plottable from any stacking, set \a bars to 0. + + \see moveBelow, barAbove, barBelow +*/ +void QCPBars::moveBelow(QCPBars *bars) +{ + if (bars == this) return; + if (bars && (bars->keyAxis() != mKeyAxis.data() || bars->valueAxis() != mValueAxis.data())) + { + qDebug() << Q_FUNC_INFO << "passed QCPBars* doesn't have same key and value axis as this QCPBars"; + return; + } + // remove from stacking: + connectBars(mBarBelow.data(), mBarAbove.data()); // Note: also works if one (or both) of them is 0 + // if new bar given, insert this bar below it: + if (bars) + { + if (bars->mBarBelow) + connectBars(bars->mBarBelow.data(), this); + connectBars(this, bars); + } +} + +/*! + Moves this bars plottable above \a bars. In other words, the bars of this plottable will appear + above the bars of \a bars. The move target \a bars must use the same key and value axis as this + plottable. + + Inserting into and removing from existing bar stacking is handled gracefully. If \a bars already + has a bars object above itself, this bars object is inserted between the two. If this bars object + is already between two other bars, the two other bars will be stacked on top of each other after + the operation. + + To remove this bars plottable from any stacking, set \a bars to 0. + + \see moveBelow, barBelow, barAbove +*/ +void QCPBars::moveAbove(QCPBars *bars) +{ + if (bars == this) return; + if (bars && (bars->keyAxis() != mKeyAxis.data() || bars->valueAxis() != mValueAxis.data())) + { + qDebug() << Q_FUNC_INFO << "passed QCPBars* doesn't have same key and value axis as this QCPBars"; + return; + } + // remove from stacking: + connectBars(mBarBelow.data(), mBarAbove.data()); // Note: also works if one (or both) of them is 0 + // if new bar given, insert this bar above it: + if (bars) + { + if (bars->mBarAbove) + connectBars(this, bars->mBarAbove.data()); + connectBars(bars, this); + } +} + +/*! + Adds the provided data points in \a dataMap to the current data. + \see removeData +*/ +void QCPBars::addData(const QCPBarDataMap &dataMap) +{ + mData->unite(dataMap); +} + +/*! \overload + Adds the provided single data point in \a data to the current data. + \see removeData +*/ +void QCPBars::addData(const QCPBarData &data) +{ + mData->insertMulti(data.key, data); +} + +/*! \overload + Adds the provided single data point as \a key and \a value tuple to the current data + \see removeData +*/ +void QCPBars::addData(double key, double value) +{ + QCPBarData newData; + newData.key = key; + newData.value = value; + mData->insertMulti(newData.key, newData); +} + +/*! \overload + Adds the provided data points as \a key and \a value tuples to the current data. + \see removeData +*/ +void QCPBars::addData(const QVector &keys, const QVector &values) +{ + int n = keys.size(); + n = qMin(n, values.size()); + QCPBarData newData; + for (int i=0; iinsertMulti(newData.key, newData); + } +} + +/*! + Removes all data points with key smaller than \a key. + \see addData, clearData +*/ +void QCPBars::removeDataBefore(double key) +{ + QCPBarDataMap::iterator it = mData->begin(); + while (it != mData->end() && it.key() < key) + it = mData->erase(it); +} + +/*! + Removes all data points with key greater than \a key. + \see addData, clearData +*/ +void QCPBars::removeDataAfter(double key) +{ + if (mData->isEmpty()) return; + QCPBarDataMap::iterator it = mData->upperBound(key); + while (it != mData->end()) + it = mData->erase(it); +} + +/*! + Removes all data points with key between \a fromKey and \a toKey. if \a fromKey is + greater or equal to \a toKey, the function does nothing. To remove a single data point with known + key, use \ref removeData(double key). + + \see addData, clearData +*/ +void QCPBars::removeData(double fromKey, double toKey) +{ + if (fromKey >= toKey || mData->isEmpty()) return; + QCPBarDataMap::iterator it = mData->upperBound(fromKey); + QCPBarDataMap::iterator itEnd = mData->upperBound(toKey); + while (it != itEnd) + it = mData->erase(it); +} + +/*! \overload + + Removes a single data point at \a key. If the position is not known with absolute precision, + consider using \ref removeData(double fromKey, double toKey) with a small fuzziness interval + around the suspected position, depeding on the precision with which the key is known. + + \see addData, clearData +*/ +void QCPBars::removeData(double key) +{ + mData->remove(key); +} + +/*! + Removes all data points. + \see removeData, removeDataAfter, removeDataBefore +*/ +void QCPBars::clearData() +{ + mData->clear(); +} + +/* inherits documentation from base class */ +double QCPBars::selectTest(const QPointF &pos, bool onlySelectable, QVariant *details) const +{ + Q_UNUSED(details) + if (onlySelectable && !mSelectable) + return -1; + if (!mKeyAxis || !mValueAxis) { qDebug() << Q_FUNC_INFO << "invalid key or value axis"; return -1; } + + if (mKeyAxis.data()->axisRect()->rect().contains(pos.toPoint())) + { + QCPBarDataMap::ConstIterator it; + for (it = mData->constBegin(); it != mData->constEnd(); ++it) + { + if (getBarPolygon(it.value().key, it.value().value).boundingRect().contains(pos)) + return mParentPlot->selectionTolerance()*0.99; + } + } + return -1; +} + +/* inherits documentation from base class */ +void QCPBars::draw(QCPPainter *painter) +{ + if (!mKeyAxis || !mValueAxis) { qDebug() << Q_FUNC_INFO << "invalid key or value axis"; return; } + if (mData->isEmpty()) return; + + QCPBarDataMap::const_iterator it, lower, upperEnd; + getVisibleDataBounds(lower, upperEnd); + for (it = lower; it != upperEnd; ++it) + { + // check data validity if flag set: +#ifdef QCUSTOMPLOT_CHECK_DATA + if (QCP::isInvalidData(it.value().key, it.value().value)) + qDebug() << Q_FUNC_INFO << "Data point at" << it.key() << "of drawn range invalid." << "Plottable name:" << name(); +#endif + QPolygonF barPolygon = getBarPolygon(it.key(), it.value().value); + // draw bar fill: + if (mainBrush().style() != Qt::NoBrush && mainBrush().color().alpha() != 0) + { + applyFillAntialiasingHint(painter); + painter->setPen(Qt::NoPen); + painter->setBrush(mainBrush()); + painter->drawPolygon(barPolygon); + } + // draw bar line: + if (mainPen().style() != Qt::NoPen && mainPen().color().alpha() != 0) + { + applyDefaultAntialiasingHint(painter); + painter->setPen(mainPen()); + painter->setBrush(Qt::NoBrush); + painter->drawPolyline(barPolygon); + } + } +} + +/* inherits documentation from base class */ +void QCPBars::drawLegendIcon(QCPPainter *painter, const QRectF &rect) const +{ + // draw filled rect: + applyDefaultAntialiasingHint(painter); + painter->setBrush(mBrush); + painter->setPen(mPen); + QRectF r = QRectF(0, 0, rect.width()*0.67, rect.height()*0.67); + r.moveCenter(rect.center()); + painter->drawRect(r); +} + +/*! \internal + + called by \ref draw to determine which data (key) range is visible at the current key axis range + setting, so only that needs to be processed. It also takes into account the bar width. + + \a lower returns an iterator to the lowest data point that needs to be taken into account when + plotting. Note that in order to get a clean plot all the way to the edge of the axis rect, \a + lower may still be just outside the visible range. + + \a upperEnd returns an iterator one higher than the highest visible data point. Same as before, \a + upperEnd may also lie just outside of the visible range. + + if the bars plottable contains no data, both \a lower and \a upperEnd point to constEnd. +*/ +void QCPBars::getVisibleDataBounds(QCPBarDataMap::const_iterator &lower, QCPBarDataMap::const_iterator &upperEnd) const +{ + if (!mKeyAxis) { qDebug() << Q_FUNC_INFO << "invalid key axis"; return; } + if (mData->isEmpty()) + { + lower = mData->constEnd(); + upperEnd = mData->constEnd(); + return; + } + + // get visible data range as QMap iterators + lower = mData->lowerBound(mKeyAxis.data()->range().lower); + upperEnd = mData->upperBound(mKeyAxis.data()->range().upper); + double lowerPixelBound = mKeyAxis.data()->coordToPixel(mKeyAxis.data()->range().lower); + double upperPixelBound = mKeyAxis.data()->coordToPixel(mKeyAxis.data()->range().upper); + bool isVisible = false; + // walk left from lbound to find lower bar that actually is completely outside visible pixel range: + QCPBarDataMap::const_iterator it = lower; + while (it != mData->constBegin()) + { + --it; + QRectF barBounds = getBarPolygon(it.value().key, it.value().value).boundingRect(); + if (mKeyAxis.data()->orientation() == Qt::Horizontal) + isVisible = ((!mKeyAxis.data()->rangeReversed() && barBounds.right() >= lowerPixelBound) || (mKeyAxis.data()->rangeReversed() && barBounds.left() <= lowerPixelBound)); + else // keyaxis is vertical + isVisible = ((!mKeyAxis.data()->rangeReversed() && barBounds.top() <= lowerPixelBound) || (mKeyAxis.data()->rangeReversed() && barBounds.bottom() >= lowerPixelBound)); + if (isVisible) + lower = it; + else + break; + } + // walk right from ubound to find upper bar that actually is completely outside visible pixel range: + it = upperEnd; + while (it != mData->constEnd()) + { + QRectF barBounds = getBarPolygon(upperEnd.value().key, upperEnd.value().value).boundingRect(); + if (mKeyAxis.data()->orientation() == Qt::Horizontal) + isVisible = ((!mKeyAxis.data()->rangeReversed() && barBounds.left() <= upperPixelBound) || (mKeyAxis.data()->rangeReversed() && barBounds.right() >= upperPixelBound)); + else // keyaxis is vertical + isVisible = ((!mKeyAxis.data()->rangeReversed() && barBounds.bottom() >= upperPixelBound) || (mKeyAxis.data()->rangeReversed() && barBounds.top() <= upperPixelBound)); + if (isVisible) + upperEnd = it+1; + else + break; + ++it; + } +} + +/*! \internal + + Returns the polygon of a single bar with \a key and \a value. The Polygon is open at the bottom + and shifted according to the bar stacking (see \ref moveAbove) and base value (see \ref + setBaseValue). +*/ +QPolygonF QCPBars::getBarPolygon(double key, double value) const +{ + QCPAxis *keyAxis = mKeyAxis.data(); + QCPAxis *valueAxis = mValueAxis.data(); + if (!keyAxis || !valueAxis) { qDebug() << Q_FUNC_INFO << "invalid key or value axis"; return QPolygonF(); } + + QPolygonF result; + double lowerPixelWidth, upperPixelWidth; + getPixelWidth(key, lowerPixelWidth, upperPixelWidth); + double base = getStackedBaseValue(key, value >= 0); + double basePixel = valueAxis->coordToPixel(base); + double valuePixel = valueAxis->coordToPixel(base+value); + double keyPixel = keyAxis->coordToPixel(key); + if (mBarsGroup) + keyPixel += mBarsGroup->keyPixelOffset(this, key); + if (keyAxis->orientation() == Qt::Horizontal) + { + result << QPointF(keyPixel+lowerPixelWidth, basePixel); + result << QPointF(keyPixel+lowerPixelWidth, valuePixel); + result << QPointF(keyPixel+upperPixelWidth, valuePixel); + result << QPointF(keyPixel+upperPixelWidth, basePixel); + } else + { + result << QPointF(basePixel, keyPixel+lowerPixelWidth); + result << QPointF(valuePixel, keyPixel+lowerPixelWidth); + result << QPointF(valuePixel, keyPixel+upperPixelWidth); + result << QPointF(basePixel, keyPixel+upperPixelWidth); + } + return result; +} + +/*! \internal + + This function is used to determine the width of the bar at coordinate \a key, according to the + specified width (\ref setWidth) and width type (\ref setWidthType). + + The output parameters \a lower and \a upper return the number of pixels the bar extends to lower + and higher keys, relative to the \a key coordinate (so with a non-reversed horizontal axis, \a + lower is negative and \a upper positive). +*/ +void QCPBars::getPixelWidth(double key, double &lower, double &upper) const +{ + switch (mWidthType) + { + case wtAbsolute: + { + upper = mWidth*0.5; + lower = -upper; + if (mKeyAxis && (mKeyAxis.data()->rangeReversed() ^ (mKeyAxis.data()->orientation() == Qt::Vertical))) + qSwap(lower, upper); + break; + } + case wtAxisRectRatio: + { + if (mKeyAxis && mKeyAxis.data()->axisRect()) + { + if (mKeyAxis.data()->orientation() == Qt::Horizontal) + upper = mKeyAxis.data()->axisRect()->width()*mWidth*0.5; + else + upper = mKeyAxis.data()->axisRect()->height()*mWidth*0.5; + lower = -upper; + if (mKeyAxis && (mKeyAxis.data()->rangeReversed() ^ (mKeyAxis.data()->orientation() == Qt::Vertical))) + qSwap(lower, upper); + } else + qDebug() << Q_FUNC_INFO << "No key axis or axis rect defined"; + break; + } + case wtPlotCoords: + { + if (mKeyAxis) + { + double keyPixel = mKeyAxis.data()->coordToPixel(key); + upper = mKeyAxis.data()->coordToPixel(key+mWidth*0.5)-keyPixel; + lower = mKeyAxis.data()->coordToPixel(key-mWidth*0.5)-keyPixel; + // no need to qSwap(lower, higher) when range reversed, because higher/lower are gained by + // coordinate transform which includes range direction + } else + qDebug() << Q_FUNC_INFO << "No key axis defined"; + break; + } + } +} + +/*! \internal + + This function is called to find at which value to start drawing the base of a bar at \a key, when + it is stacked on top of another QCPBars (e.g. with \ref moveAbove). + + positive and negative bars are separated per stack (positive are stacked above baseValue upwards, + negative are stacked below baseValue downwards). This can be indicated with \a positive. So if the + bar for which we need the base value is negative, set \a positive to false. +*/ +double QCPBars::getStackedBaseValue(double key, bool positive) const +{ + if (mBarBelow) + { + double max = 0; // don't use mBaseValue here because only base value of bottom-most bar has meaning in a bar stack + // find bars of mBarBelow that are approximately at key and find largest one: + double epsilon = qAbs(key)*1e-6; // should be safe even when changed to use float at some point + if (key == 0) + epsilon = 1e-6; + QCPBarDataMap::const_iterator it = mBarBelow.data()->mData->lowerBound(key-epsilon); + QCPBarDataMap::const_iterator itEnd = mBarBelow.data()->mData->upperBound(key+epsilon); + while (it != itEnd) + { + if ((positive && it.value().value > max) || + (!positive && it.value().value < max)) + max = it.value().value; + ++it; + } + // recurse down the bar-stack to find the total height: + return max + mBarBelow.data()->getStackedBaseValue(key, positive); + } else + return mBaseValue; +} + +/*! \internal + + Connects \a below and \a above to each other via their mBarAbove/mBarBelow properties. The bar(s) + currently above lower and below upper will become disconnected to lower/upper. + + If lower is zero, upper will be disconnected at the bottom. + If upper is zero, lower will be disconnected at the top. +*/ +void QCPBars::connectBars(QCPBars *lower, QCPBars *upper) +{ + if (!lower && !upper) return; + + if (!lower) // disconnect upper at bottom + { + // disconnect old bar below upper: + if (upper->mBarBelow && upper->mBarBelow.data()->mBarAbove.data() == upper) + upper->mBarBelow.data()->mBarAbove = 0; + upper->mBarBelow = 0; + } else if (!upper) // disconnect lower at top + { + // disconnect old bar above lower: + if (lower->mBarAbove && lower->mBarAbove.data()->mBarBelow.data() == lower) + lower->mBarAbove.data()->mBarBelow = 0; + lower->mBarAbove = 0; + } else // connect lower and upper + { + // disconnect old bar above lower: + if (lower->mBarAbove && lower->mBarAbove.data()->mBarBelow.data() == lower) + lower->mBarAbove.data()->mBarBelow = 0; + // disconnect old bar below upper: + if (upper->mBarBelow && upper->mBarBelow.data()->mBarAbove.data() == upper) + upper->mBarBelow.data()->mBarAbove = 0; + lower->mBarAbove = upper; + upper->mBarBelow = lower; + } +} + +/* inherits documentation from base class */ +QCPRange QCPBars::getKeyRange(bool &foundRange, SignDomain inSignDomain) const +{ + QCPRange range; + bool haveLower = false; + bool haveUpper = false; + + double current; + QCPBarDataMap::const_iterator it = mData->constBegin(); + while (it != mData->constEnd()) + { + current = it.value().key; + if (inSignDomain == sdBoth || (inSignDomain == sdNegative && current < 0) || (inSignDomain == sdPositive && current > 0)) + { + if (current < range.lower || !haveLower) + { + range.lower = current; + haveLower = true; + } + if (current > range.upper || !haveUpper) + { + range.upper = current; + haveUpper = true; + } + } + ++it; + } + // determine exact range of bars by including bar width and barsgroup offset: + if (haveLower && mKeyAxis) + { + double lowerPixelWidth, upperPixelWidth, keyPixel; + getPixelWidth(range.lower, lowerPixelWidth, upperPixelWidth); + keyPixel = mKeyAxis.data()->coordToPixel(range.lower) + lowerPixelWidth; + if (mBarsGroup) + keyPixel += mBarsGroup->keyPixelOffset(this, range.lower); + range.lower = mKeyAxis.data()->pixelToCoord(keyPixel); + } + if (haveUpper && mKeyAxis) + { + double lowerPixelWidth, upperPixelWidth, keyPixel; + getPixelWidth(range.upper, lowerPixelWidth, upperPixelWidth); + keyPixel = mKeyAxis.data()->coordToPixel(range.upper) + upperPixelWidth; + if (mBarsGroup) + keyPixel += mBarsGroup->keyPixelOffset(this, range.upper); + range.upper = mKeyAxis.data()->pixelToCoord(keyPixel); + } + foundRange = haveLower && haveUpper; + return range; +} + +/* inherits documentation from base class */ +QCPRange QCPBars::getValueRange(bool &foundRange, SignDomain inSignDomain) const +{ + QCPRange range; + range.lower = mBaseValue; + range.upper = mBaseValue; + bool haveLower = true; // set to true, because baseValue should always be visible in bar charts + bool haveUpper = true; // set to true, because baseValue should always be visible in bar charts + double current; + + QCPBarDataMap::const_iterator it = mData->constBegin(); + while (it != mData->constEnd()) + { + current = it.value().value + getStackedBaseValue(it.value().key, it.value().value >= 0); + if (inSignDomain == sdBoth || (inSignDomain == sdNegative && current < 0) || (inSignDomain == sdPositive && current > 0)) + { + if (current < range.lower || !haveLower) + { + range.lower = current; + haveLower = true; + } + if (current > range.upper || !haveUpper) + { + range.upper = current; + haveUpper = true; + } + } + ++it; + } + + foundRange = true; // return true because bar charts always have the 0-line visible + return range; +} + + +//////////////////////////////////////////////////////////////////////////////////////////////////// +//////////////////// QCPStatisticalBox +//////////////////////////////////////////////////////////////////////////////////////////////////// + +/*! \class QCPStatisticalBox + \brief A plottable representing a single statistical box in a plot. + + \image html QCPStatisticalBox.png + + To plot data, assign it with the individual parameter functions or use \ref setData to set all + parameters at once. The individual functions are: + \li \ref setMinimum + \li \ref setLowerQuartile + \li \ref setMedian + \li \ref setUpperQuartile + \li \ref setMaximum + + Additionally you can define a list of outliers, drawn as scatter datapoints: + \li \ref setOutliers + + \section appearance Changing the appearance + + The appearance of the box itself is controlled via \ref setPen and \ref setBrush. You may change + the width of the box with \ref setWidth in plot coordinates (not pixels). + + Analog functions exist for the minimum/maximum-whiskers: \ref setWhiskerPen, \ref + setWhiskerBarPen, \ref setWhiskerWidth. The whisker width is the width of the bar at the top + (maximum) and bottom (minimum). + + The median indicator line has its own pen, \ref setMedianPen. + + If the whisker backbone pen is changed, make sure to set the capStyle to Qt::FlatCap. Else, the + backbone line might exceed the whisker bars by a few pixels due to the pen cap being not + perfectly flat. + + The Outlier data points are drawn as normal scatter points. Their look can be controlled with + \ref setOutlierStyle + + \section usage Usage + + Like all data representing objects in QCustomPlot, the QCPStatisticalBox is a plottable. + Usually, you first create an instance and add it to the customPlot: + \snippet documentation/doc-code-snippets/mainwindow.cpp qcpstatisticalbox-creation-1 + and then modify the properties of the newly created plottable, e.g.: + \snippet documentation/doc-code-snippets/mainwindow.cpp qcpstatisticalbox-creation-2 +*/ + +/*! + Constructs a statistical box which uses \a keyAxis as its key axis ("x") and \a valueAxis as its + value axis ("y"). \a keyAxis and \a valueAxis must reside in the same QCustomPlot instance and + not have the same orientation. If either of these restrictions is violated, a corresponding + message is printed to the debug output (qDebug), the construction is not aborted, though. + + The constructed statistical box can be added to the plot with QCustomPlot::addPlottable, + QCustomPlot then takes ownership of the statistical box. +*/ +QCPStatisticalBox::QCPStatisticalBox(QCPAxis *keyAxis, QCPAxis *valueAxis) : + QCPAbstractPlottable(keyAxis, valueAxis), + mKey(0), + mMinimum(0), + mLowerQuartile(0), + mMedian(0), + mUpperQuartile(0), + mMaximum(0) +{ + setOutlierStyle(QCPScatterStyle(QCPScatterStyle::ssCircle, Qt::blue, 6)); + setWhiskerWidth(0.2); + setWidth(0.5); + + setPen(QPen(Qt::black)); + setSelectedPen(QPen(Qt::blue, 2.5)); + setMedianPen(QPen(Qt::black, 3, Qt::SolidLine, Qt::FlatCap)); + setWhiskerPen(QPen(Qt::black, 0, Qt::DashLine, Qt::FlatCap)); + setWhiskerBarPen(QPen(Qt::black)); + setBrush(Qt::NoBrush); + setSelectedBrush(Qt::NoBrush); +} + +/*! + Sets the key coordinate of the statistical box. +*/ +void QCPStatisticalBox::setKey(double key) +{ + mKey = key; +} + +/*! + Sets the parameter "minimum" of the statistical box plot. This is the position of the lower + whisker, typically the minimum measurement of the sample that's not considered an outlier. + + \see setMaximum, setWhiskerPen, setWhiskerBarPen, setWhiskerWidth +*/ +void QCPStatisticalBox::setMinimum(double value) +{ + mMinimum = value; +} + +/*! + Sets the parameter "lower Quartile" of the statistical box plot. This is the lower end of the + box. The lower and the upper quartiles are the two statistical quartiles around the median of the + sample, they contain 50% of the sample data. + + \see setUpperQuartile, setPen, setBrush, setWidth +*/ +void QCPStatisticalBox::setLowerQuartile(double value) +{ + mLowerQuartile = value; +} + +/*! + Sets the parameter "median" of the statistical box plot. This is the value of the median mark + inside the quartile box. The median separates the sample data in half (50% of the sample data is + below/above the median). + + \see setMedianPen +*/ +void QCPStatisticalBox::setMedian(double value) +{ + mMedian = value; +} + +/*! + Sets the parameter "upper Quartile" of the statistical box plot. This is the upper end of the + box. The lower and the upper quartiles are the two statistical quartiles around the median of the + sample, they contain 50% of the sample data. + + \see setLowerQuartile, setPen, setBrush, setWidth +*/ +void QCPStatisticalBox::setUpperQuartile(double value) +{ + mUpperQuartile = value; +} + +/*! + Sets the parameter "maximum" of the statistical box plot. This is the position of the upper + whisker, typically the maximum measurement of the sample that's not considered an outlier. + + \see setMinimum, setWhiskerPen, setWhiskerBarPen, setWhiskerWidth +*/ +void QCPStatisticalBox::setMaximum(double value) +{ + mMaximum = value; +} + +/*! + Sets a vector of outlier values that will be drawn as scatters. Any data points in the sample + that are not within the whiskers (\ref setMinimum, \ref setMaximum) should be considered outliers + and displayed as such. + + \see setOutlierStyle +*/ +void QCPStatisticalBox::setOutliers(const QVector &values) +{ + mOutliers = values; +} + +/*! + Sets all parameters of the statistical box plot at once. + + \see setKey, setMinimum, setLowerQuartile, setMedian, setUpperQuartile, setMaximum +*/ +void QCPStatisticalBox::setData(double key, double minimum, double lowerQuartile, double median, double upperQuartile, double maximum) +{ + setKey(key); + setMinimum(minimum); + setLowerQuartile(lowerQuartile); + setMedian(median); + setUpperQuartile(upperQuartile); + setMaximum(maximum); +} + +/*! + Sets the width of the box in key coordinates. + + \see setWhiskerWidth +*/ +void QCPStatisticalBox::setWidth(double width) +{ + mWidth = width; +} + +/*! + Sets the width of the whiskers (\ref setMinimum, \ref setMaximum) in key coordinates. + + \see setWidth +*/ +void QCPStatisticalBox::setWhiskerWidth(double width) +{ + mWhiskerWidth = width; +} + +/*! + Sets the pen used for drawing the whisker backbone (That's the line parallel to the value axis). + + Make sure to set the \a pen capStyle to Qt::FlatCap to prevent the whisker backbone from reaching + a few pixels past the whisker bars, when using a non-zero pen width. + + \see setWhiskerBarPen +*/ +void QCPStatisticalBox::setWhiskerPen(const QPen &pen) +{ + mWhiskerPen = pen; +} + +/*! + Sets the pen used for drawing the whisker bars (Those are the lines parallel to the key axis at + each end of the whisker backbone). + + \see setWhiskerPen +*/ +void QCPStatisticalBox::setWhiskerBarPen(const QPen &pen) +{ + mWhiskerBarPen = pen; +} + +/*! + Sets the pen used for drawing the median indicator line inside the statistical box. +*/ +void QCPStatisticalBox::setMedianPen(const QPen &pen) +{ + mMedianPen = pen; +} + +/*! + Sets the appearance of the outlier data points. + + \see setOutliers +*/ +void QCPStatisticalBox::setOutlierStyle(const QCPScatterStyle &style) +{ + mOutlierStyle = style; +} + +/* inherits documentation from base class */ +void QCPStatisticalBox::clearData() +{ + setOutliers(QVector()); + setKey(0); + setMinimum(0); + setLowerQuartile(0); + setMedian(0); + setUpperQuartile(0); + setMaximum(0); +} + +/* inherits documentation from base class */ +double QCPStatisticalBox::selectTest(const QPointF &pos, bool onlySelectable, QVariant *details) const +{ + Q_UNUSED(details) + if (onlySelectable && !mSelectable) + return -1; + if (!mKeyAxis || !mValueAxis) { qDebug() << Q_FUNC_INFO << "invalid key or value axis"; return -1; } + + if (mKeyAxis.data()->axisRect()->rect().contains(pos.toPoint())) + { + double posKey, posValue; + pixelsToCoords(pos, posKey, posValue); + // quartile box: + QCPRange keyRange(mKey-mWidth*0.5, mKey+mWidth*0.5); + QCPRange valueRange(mLowerQuartile, mUpperQuartile); + if (keyRange.contains(posKey) && valueRange.contains(posValue)) + return mParentPlot->selectionTolerance()*0.99; + + // min/max whiskers: + if (QCPRange(mMinimum, mMaximum).contains(posValue)) + return qAbs(mKeyAxis.data()->coordToPixel(mKey)-mKeyAxis.data()->coordToPixel(posKey)); + } + return -1; +} + +/* inherits documentation from base class */ +void QCPStatisticalBox::draw(QCPPainter *painter) +{ + if (!mKeyAxis || !mValueAxis) { qDebug() << Q_FUNC_INFO << "invalid key or value axis"; return; } + + // check data validity if flag set: +#ifdef QCUSTOMPLOT_CHECK_DATA + if (QCP::isInvalidData(mKey, mMedian) || + QCP::isInvalidData(mLowerQuartile, mUpperQuartile) || + QCP::isInvalidData(mMinimum, mMaximum)) + qDebug() << Q_FUNC_INFO << "Data point at" << mKey << "of drawn range has invalid data." << "Plottable name:" << name(); + for (int i=0; isave(); + painter->setClipRect(quartileBox, Qt::IntersectClip); + drawMedian(painter); + painter->restore(); + + drawWhiskers(painter); + drawOutliers(painter); +} + +/* inherits documentation from base class */ +void QCPStatisticalBox::drawLegendIcon(QCPPainter *painter, const QRectF &rect) const +{ + // draw filled rect: + applyDefaultAntialiasingHint(painter); + painter->setPen(mPen); + painter->setBrush(mBrush); + QRectF r = QRectF(0, 0, rect.width()*0.67, rect.height()*0.67); + r.moveCenter(rect.center()); + painter->drawRect(r); +} + +/*! \internal + + Draws the quartile box. \a box is an output parameter that returns the quartile box (in pixel + coordinates) which is used to set the clip rect of the painter before calling \ref drawMedian (so + the median doesn't draw outside the quartile box). +*/ +void QCPStatisticalBox::drawQuartileBox(QCPPainter *painter, QRectF *quartileBox) const +{ + QRectF box; + box.setTopLeft(coordsToPixels(mKey-mWidth*0.5, mUpperQuartile)); + box.setBottomRight(coordsToPixels(mKey+mWidth*0.5, mLowerQuartile)); + applyDefaultAntialiasingHint(painter); + painter->setPen(mainPen()); + painter->setBrush(mainBrush()); + painter->drawRect(box); + if (quartileBox) + *quartileBox = box; +} + +/*! \internal + + Draws the median line inside the quartile box. +*/ +void QCPStatisticalBox::drawMedian(QCPPainter *painter) const +{ + QLineF medianLine; + medianLine.setP1(coordsToPixels(mKey-mWidth*0.5, mMedian)); + medianLine.setP2(coordsToPixels(mKey+mWidth*0.5, mMedian)); + applyDefaultAntialiasingHint(painter); + painter->setPen(mMedianPen); + painter->drawLine(medianLine); +} + +/*! \internal + + Draws both whisker backbones and bars. +*/ +void QCPStatisticalBox::drawWhiskers(QCPPainter *painter) const +{ + QLineF backboneMin, backboneMax, barMin, barMax; + backboneMax.setPoints(coordsToPixels(mKey, mUpperQuartile), coordsToPixels(mKey, mMaximum)); + backboneMin.setPoints(coordsToPixels(mKey, mLowerQuartile), coordsToPixels(mKey, mMinimum)); + barMax.setPoints(coordsToPixels(mKey-mWhiskerWidth*0.5, mMaximum), coordsToPixels(mKey+mWhiskerWidth*0.5, mMaximum)); + barMin.setPoints(coordsToPixels(mKey-mWhiskerWidth*0.5, mMinimum), coordsToPixels(mKey+mWhiskerWidth*0.5, mMinimum)); + applyErrorBarsAntialiasingHint(painter); + painter->setPen(mWhiskerPen); + painter->drawLine(backboneMin); + painter->drawLine(backboneMax); + painter->setPen(mWhiskerBarPen); + painter->drawLine(barMin); + painter->drawLine(barMax); +} + +/*! \internal + + Draws the outlier scatter points. +*/ +void QCPStatisticalBox::drawOutliers(QCPPainter *painter) const +{ + applyScattersAntialiasingHint(painter); + mOutlierStyle.applyTo(painter, mPen); + for (int i=0; i 0) + return QCPRange(mKey-mWidth*0.5, mKey+mWidth*0.5); + else if (mKey > 0) + return QCPRange(mKey, mKey+mWidth*0.5); + else + { + foundRange = false; + return QCPRange(); + } + } + foundRange = false; + return QCPRange(); +} + +/* inherits documentation from base class */ +QCPRange QCPStatisticalBox::getValueRange(bool &foundRange, SignDomain inSignDomain) const +{ + QVector values; // values that must be considered (i.e. all outliers and the five box-parameters) + values.reserve(mOutliers.size() + 5); + values << mMaximum << mUpperQuartile << mMedian << mLowerQuartile << mMinimum; + values << mOutliers; + // go through values and find the ones in legal range: + bool haveUpper = false; + bool haveLower = false; + double upper = 0; + double lower = 0; + for (int i=0; i 0) || + (inSignDomain == sdBoth)) + { + if (values.at(i) > upper || !haveUpper) + { + upper = values.at(i); + haveUpper = true; + } + if (values.at(i) < lower || !haveLower) + { + lower = values.at(i); + haveLower = true; + } + } + } + // return the bounds if we found some sensible values: + if (haveLower && haveUpper) + { + foundRange = true; + return QCPRange(lower, upper); + } else // might happen if all values are in other sign domain + { + foundRange = false; + return QCPRange(); + } +} + + +//////////////////////////////////////////////////////////////////////////////////////////////////// +//////////////////// QCPColorMapData +//////////////////////////////////////////////////////////////////////////////////////////////////// + +/*! \class QCPColorMapData + \brief Holds the two-dimensional data of a QCPColorMap plottable. + + This class is a data storage for \ref QCPColorMap. It holds a two-dimensional array, which \ref + QCPColorMap then displays as a 2D image in the plot, where the array values are represented by a + color, depending on the value. + + The size of the array can be controlled via \ref setSize (or \ref setKeySize, \ref setValueSize). + Which plot coordinates these cells correspond to can be configured with \ref setRange (or \ref + setKeyRange, \ref setValueRange). + + The data cells can be accessed in two ways: They can be directly addressed by an integer index + with \ref setCell. This is the fastest method. Alternatively, they can be addressed by their plot + coordinate with \ref setData. plot coordinate to cell index transformations and vice versa are + provided by the functions \ref coordToCell and \ref cellToCoord. + + This class also buffers the minimum and maximum values that are in the data set, to provide + QCPColorMap::rescaleDataRange with the necessary information quickly. Setting a cell to a value + that is greater than the current maximum increases this maximum to the new value. However, + setting the cell that currently holds the maximum value to a smaller value doesn't decrease the + maximum again, because finding the true new maximum would require going through the entire data + array, which might be time consuming. The same holds for the data minimum. This functionality is + given by \ref recalculateDataBounds, such that you can decide when it is sensible to find the + true current minimum and maximum. The method QCPColorMap::rescaleDataRange offers a convenience + parameter \a recalculateDataBounds which may be set to true to automatically call \ref + recalculateDataBounds internally. +*/ + +/* start of documentation of inline functions */ + +/*! \fn bool QCPColorMapData::isEmpty() const + + Returns whether this instance carries no data. This is equivalent to having a size where at least + one of the dimensions is 0 (see \ref setSize). +*/ + +/* end of documentation of inline functions */ + +/*! + Constructs a new QCPColorMapData instance. The instance has \a keySize cells in the key direction + and \a valueSize cells in the value direction. These cells will be displayed by the \ref QCPColorMap + at the coordinates \a keyRange and \a valueRange. + + \see setSize, setKeySize, setValueSize, setRange, setKeyRange, setValueRange +*/ +QCPColorMapData::QCPColorMapData(int keySize, int valueSize, const QCPRange &keyRange, const QCPRange &valueRange) : + mKeySize(0), + mValueSize(0), + mKeyRange(keyRange), + mValueRange(valueRange), + mIsEmpty(true), + mData(0), + mDataModified(true) +{ + setSize(keySize, valueSize); + fill(0); +} + +QCPColorMapData::~QCPColorMapData() +{ + if (mData) + delete[] mData; +} + +/*! + Constructs a new QCPColorMapData instance copying the data and range of \a other. +*/ +QCPColorMapData::QCPColorMapData(const QCPColorMapData &other) : + mKeySize(0), + mValueSize(0), + mIsEmpty(true), + mData(0), + mDataModified(true) +{ + *this = other; +} + +/*! + Overwrites this color map data instance with the data stored in \a other. +*/ +QCPColorMapData &QCPColorMapData::operator=(const QCPColorMapData &other) +{ + if (&other != this) + { + const int keySize = other.keySize(); + const int valueSize = other.valueSize(); + setSize(keySize, valueSize); + setRange(other.keyRange(), other.valueRange()); + if (!mIsEmpty) + memcpy(mData, other.mData, sizeof(mData[0])*keySize*valueSize); + mDataBounds = other.mDataBounds; + mDataModified = true; + } + return *this; +} + +/* undocumented getter */ +double QCPColorMapData::data(double key, double value) +{ + int keyCell = (key-mKeyRange.lower)/(mKeyRange.upper-mKeyRange.lower)*(mKeySize-1)+0.5; + int valueCell = (value-mValueRange.lower)/(mValueRange.upper-mValueRange.lower)*(mValueSize-1)+0.5; + if (keyCell >= 0 && keyCell < mKeySize && valueCell >= 0 && valueCell < mValueSize) + return mData[valueCell*mKeySize + keyCell]; + else + return 0; +} + +/* undocumented getter */ +double QCPColorMapData::cell(int keyIndex, int valueIndex) +{ + if (keyIndex >= 0 && keyIndex < mKeySize && valueIndex >= 0 && valueIndex < mValueSize) + return mData[valueIndex*mKeySize + keyIndex]; + else + return 0; +} + +/*! + Resizes the data array to have \a keySize cells in the key dimension and \a valueSize cells in + the value dimension. + + The current data is discarded and the map cells are set to 0, unless the map had already the + requested size. + + Setting at least one of \a keySize or \a valueSize to zero frees the internal data array and \ref + isEmpty returns true. + + \see setRange, setKeySize, setValueSize +*/ +void QCPColorMapData::setSize(int keySize, int valueSize) +{ + if (keySize != mKeySize || valueSize != mValueSize) + { + mKeySize = keySize; + mValueSize = valueSize; + if (mData) + delete[] mData; + mIsEmpty = mKeySize == 0 || mValueSize == 0; + if (!mIsEmpty) + { +#ifdef __EXCEPTIONS + try { // 2D arrays get memory intensive fast. So if the allocation fails, at least output debug message +#endif + mData = new double[mKeySize*mValueSize]; +#ifdef __EXCEPTIONS + } catch (...) { mData = 0; } +#endif + if (mData) + fill(0); + else + qDebug() << Q_FUNC_INFO << "out of memory for data dimensions "<< mKeySize << "*" << mValueSize; + } else + mData = 0; + mDataModified = true; + } +} + +/*! + Resizes the data array to have \a keySize cells in the key dimension. + + The current data is discarded and the map cells are set to 0, unless the map had already the + requested size. + + Setting \a keySize to zero frees the internal data array and \ref isEmpty returns true. + + \see setKeyRange, setSize, setValueSize +*/ +void QCPColorMapData::setKeySize(int keySize) +{ + setSize(keySize, mValueSize); +} + +/*! + Resizes the data array to have \a valueSize cells in the value dimension. + + The current data is discarded and the map cells are set to 0, unless the map had already the + requested size. + + Setting \a valueSize to zero frees the internal data array and \ref isEmpty returns true. + + \see setValueRange, setSize, setKeySize +*/ +void QCPColorMapData::setValueSize(int valueSize) +{ + setSize(mKeySize, valueSize); +} + +/*! + Sets the coordinate ranges the data shall be distributed over. This defines the rectangular area + covered by the color map in plot coordinates. + + The outer cells will be centered on the range boundaries given to this function. For example, if + the key size (\ref setKeySize) is 3 and \a keyRange is set to QCPRange(2, 3) there will + be cells centered on the key coordinates 2, 2.5 and 3. + + \see setSize +*/ +void QCPColorMapData::setRange(const QCPRange &keyRange, const QCPRange &valueRange) +{ + setKeyRange(keyRange); + setValueRange(valueRange); +} + +/*! + Sets the coordinate range the data shall be distributed over in the key dimension. Together with + the value range, This defines the rectangular area covered by the color map in plot coordinates. + + The outer cells will be centered on the range boundaries given to this function. For example, if + the key size (\ref setKeySize) is 3 and \a keyRange is set to QCPRange(2, 3) there will + be cells centered on the key coordinates 2, 2.5 and 3. + + \see setRange, setValueRange, setSize +*/ +void QCPColorMapData::setKeyRange(const QCPRange &keyRange) +{ + mKeyRange = keyRange; +} + +/*! + Sets the coordinate range the data shall be distributed over in the value dimension. Together with + the key range, This defines the rectangular area covered by the color map in plot coordinates. + + The outer cells will be centered on the range boundaries given to this function. For example, if + the value size (\ref setValueSize) is 3 and \a valueRange is set to QCPRange(2, 3) there + will be cells centered on the value coordinates 2, 2.5 and 3. + + \see setRange, setKeyRange, setSize +*/ +void QCPColorMapData::setValueRange(const QCPRange &valueRange) +{ + mValueRange = valueRange; +} + +/*! + Sets the data of the cell, which lies at the plot coordinates given by \a key and \a value, to \a + z. + + \note The QCPColorMap always displays the data at equal key/value intervals, even if the key or + value axis is set to a logarithmic scaling. If you want to use QCPColorMap with logarithmic axes, + you shouldn't use the \ref QCPColorMapData::setData method as it uses a linear transformation to + determine the cell index. Rather directly access the cell index with \ref + QCPColorMapData::setCell. + + \see setCell, setRange +*/ +void QCPColorMapData::setData(double key, double value, double z) +{ + int keyCell = (key-mKeyRange.lower)/(mKeyRange.upper-mKeyRange.lower)*(mKeySize-1)+0.5; + int valueCell = (value-mValueRange.lower)/(mValueRange.upper-mValueRange.lower)*(mValueSize-1)+0.5; + if (keyCell >= 0 && keyCell < mKeySize && valueCell >= 0 && valueCell < mValueSize) + { + mData[valueCell*mKeySize + keyCell] = z; + if (z < mDataBounds.lower) + mDataBounds.lower = z; + if (z > mDataBounds.upper) + mDataBounds.upper = z; + mDataModified = true; + } +} + +/*! + Sets the data of the cell with indices \a keyIndex and \a valueIndex to \a z. The indices + enumerate the cells starting from zero, up to the map's size-1 in the respective dimension (see + \ref setSize). + + In the standard plot configuration (horizontal key axis and vertical value axis, both not + range-reversed), the cell with indices (0, 0) is in the bottom left corner and the cell with + indices (keySize-1, valueSize-1) is in the top right corner of the color map. + + \see setData, setSize +*/ +void QCPColorMapData::setCell(int keyIndex, int valueIndex, double z) +{ + if (keyIndex >= 0 && keyIndex < mKeySize && valueIndex >= 0 && valueIndex < mValueSize) + { + mData[valueIndex*mKeySize + keyIndex] = z; + if (z < mDataBounds.lower) + mDataBounds.lower = z; + if (z > mDataBounds.upper) + mDataBounds.upper = z; + mDataModified = true; + } +} + +/*! + Goes through the data and updates the buffered minimum and maximum data values. + + Calling this method is only advised if you are about to call \ref QCPColorMap::rescaleDataRange + and can not guarantee that the cells holding the maximum or minimum data haven't been overwritten + with a smaller or larger value respectively, since the buffered maximum/minimum values have been + updated the last time. Why this is the case is explained in the class description (\ref + QCPColorMapData). + + Note that the method \ref QCPColorMap::rescaleDataRange provides a parameter \a + recalculateDataBounds for convenience. Setting this to true will call this method for you, before + doing the rescale. +*/ +void QCPColorMapData::recalculateDataBounds() +{ + if (mKeySize > 0 && mValueSize > 0) + { + double minHeight = mData[0]; + double maxHeight = mData[0]; + const int dataCount = mValueSize*mKeySize; + for (int i=0; i maxHeight) + maxHeight = mData[i]; + if (mData[i] < minHeight) + minHeight = mData[i]; + } + mDataBounds.lower = minHeight; + mDataBounds.upper = maxHeight; + } +} + +/*! + Frees the internal data memory. + + This is equivalent to calling \ref setSize "setSize(0, 0)". +*/ +void QCPColorMapData::clear() +{ + setSize(0, 0); +} + +/*! + Sets all cells to the value \a z. +*/ +void QCPColorMapData::fill(double z) +{ + const int dataCount = mValueSize*mKeySize; + for (int i=0; i(data); + return; + } + if (copy) + { + *mMapData = *data; + } else + { + delete mMapData; + mMapData = data; + } + mMapImageInvalidated = true; +} + +/*! + Sets the data range of this color map to \a dataRange. The data range defines which data values + are mapped to the color gradient. + + To make the data range span the full range of the data set, use \ref rescaleDataRange. + + \see QCPColorScale::setDataRange +*/ +void QCPColorMap::setDataRange(const QCPRange &dataRange) +{ + if (!QCPRange::validRange(dataRange)) return; + if (mDataRange.lower != dataRange.lower || mDataRange.upper != dataRange.upper) + { + if (mDataScaleType == QCPAxis::stLogarithmic) + mDataRange = dataRange.sanitizedForLogScale(); + else + mDataRange = dataRange.sanitizedForLinScale(); + mMapImageInvalidated = true; + emit dataRangeChanged(mDataRange); + } +} + +/*! + Sets whether the data is correlated with the color gradient linearly or logarithmically. + + \see QCPColorScale::setDataScaleType +*/ +void QCPColorMap::setDataScaleType(QCPAxis::ScaleType scaleType) +{ + if (mDataScaleType != scaleType) + { + mDataScaleType = scaleType; + mMapImageInvalidated = true; + emit dataScaleTypeChanged(mDataScaleType); + if (mDataScaleType == QCPAxis::stLogarithmic) + setDataRange(mDataRange.sanitizedForLogScale()); + } +} + +/*! + Sets the color gradient that is used to represent the data. For more details on how to create an + own gradient or use one of the preset gradients, see \ref QCPColorGradient. + + The colors defined by the gradient will be used to represent data values in the currently set + data range, see \ref setDataRange. Data points that are outside this data range will either be + colored uniformly with the respective gradient boundary color, or the gradient will repeat, + depending on \ref QCPColorGradient::setPeriodic. + + \see QCPColorScale::setGradient +*/ +void QCPColorMap::setGradient(const QCPColorGradient &gradient) +{ + if (mGradient != gradient) + { + mGradient = gradient; + mMapImageInvalidated = true; + emit gradientChanged(mGradient); + } +} + +/*! + Sets whether the color map image shall use bicubic interpolation when displaying the color map + shrinked or expanded, and not at a 1:1 pixel-to-data scale. + + \image html QCPColorMap-interpolate.png "A 10*10 color map, with interpolation and without interpolation enabled" +*/ +void QCPColorMap::setInterpolate(bool enabled) +{ + mInterpolate = enabled; + mMapImageInvalidated = true; // because oversampling factors might need to change +} + +/*! + Sets whether the outer most data rows and columns are clipped to the specified key and value + range (see \ref QCPColorMapData::setKeyRange, \ref QCPColorMapData::setValueRange). + + if \a enabled is set to false, the data points at the border of the color map are drawn with the + same width and height as all other data points. Since the data points are represented by + rectangles of one color centered on the data coordinate, this means that the shown color map + extends by half a data point over the specified key/value range in each direction. + + \image html QCPColorMap-tightboundary.png "A color map, with tight boundary enabled and disabled" +*/ +void QCPColorMap::setTightBoundary(bool enabled) +{ + mTightBoundary = enabled; +} + +/*! + Associates the color scale \a colorScale with this color map. + + This means that both the color scale and the color map synchronize their gradient, data range and + data scale type (\ref setGradient, \ref setDataRange, \ref setDataScaleType). Multiple color maps + can be associated with one single color scale. This causes the color maps to also synchronize + those properties, via the mutual color scale. + + This function causes the color map to adopt the current color gradient, data range and data scale + type of \a colorScale. After this call, you may change these properties at either the color map + or the color scale, and the setting will be applied to both. + + Pass 0 as \a colorScale to disconnect the color scale from this color map again. +*/ +void QCPColorMap::setColorScale(QCPColorScale *colorScale) +{ + if (mColorScale) // unconnect signals from old color scale + { + disconnect(this, SIGNAL(dataRangeChanged(QCPRange)), mColorScale.data(), SLOT(setDataRange(QCPRange))); + disconnect(this, SIGNAL(dataScaleTypeChanged(QCPAxis::ScaleType)), mColorScale.data(), SLOT(setDataScaleType(QCPAxis::ScaleType))); + disconnect(this, SIGNAL(gradientChanged(QCPColorGradient)), mColorScale.data(), SLOT(setGradient(QCPColorGradient))); + disconnect(mColorScale.data(), SIGNAL(dataRangeChanged(QCPRange)), this, SLOT(setDataRange(QCPRange))); + disconnect(mColorScale.data(), SIGNAL(gradientChanged(QCPColorGradient)), this, SLOT(setGradient(QCPColorGradient))); + disconnect(mColorScale.data(), SIGNAL(dataScaleTypeChanged(QCPAxis::ScaleType)), this, SLOT(setDataScaleType(QCPAxis::ScaleType))); + } + mColorScale = colorScale; + if (mColorScale) // connect signals to new color scale + { + setGradient(mColorScale.data()->gradient()); + setDataRange(mColorScale.data()->dataRange()); + setDataScaleType(mColorScale.data()->dataScaleType()); + connect(this, SIGNAL(dataRangeChanged(QCPRange)), mColorScale.data(), SLOT(setDataRange(QCPRange))); + connect(this, SIGNAL(dataScaleTypeChanged(QCPAxis::ScaleType)), mColorScale.data(), SLOT(setDataScaleType(QCPAxis::ScaleType))); + connect(this, SIGNAL(gradientChanged(QCPColorGradient)), mColorScale.data(), SLOT(setGradient(QCPColorGradient))); + connect(mColorScale.data(), SIGNAL(dataRangeChanged(QCPRange)), this, SLOT(setDataRange(QCPRange))); + connect(mColorScale.data(), SIGNAL(gradientChanged(QCPColorGradient)), this, SLOT(setGradient(QCPColorGradient))); + connect(mColorScale.data(), SIGNAL(dataScaleTypeChanged(QCPAxis::ScaleType)), this, SLOT(setDataScaleType(QCPAxis::ScaleType))); + } +} + +/*! + Sets the data range (\ref setDataRange) to span the minimum and maximum values that occur in the + current data set. This corresponds to the \ref rescaleKeyAxis or \ref rescaleValueAxis methods, + only for the third data dimension of the color map. + + The minimum and maximum values of the data set are buffered in the internal QCPColorMapData + instance (\ref data). As data is updated via its \ref QCPColorMapData::setCell or \ref + QCPColorMapData::setData, the buffered minimum and maximum values are updated, too. For + performance reasons, however, they are only updated in an expanding fashion. So the buffered + maximum can only increase and the buffered minimum can only decrease. In consequence, changes to + the data that actually lower the maximum of the data set (by overwriting the cell holding the + current maximum with a smaller value), aren't recognized and the buffered maximum overestimates + the true maximum of the data set. The same happens for the buffered minimum. To recalculate the + true minimum and maximum by explicitly looking at each cell, the method + QCPColorMapData::recalculateDataBounds can be used. For convenience, setting the parameter \a + recalculateDataBounds calls this method before setting the data range to the buffered minimum and + maximum. + + \see setDataRange +*/ +void QCPColorMap::rescaleDataRange(bool recalculateDataBounds) +{ + if (recalculateDataBounds) + mMapData->recalculateDataBounds(); + setDataRange(mMapData->dataBounds()); +} + +/*! + Takes the current appearance of the color map and updates the legend icon, which is used to + represent this color map in the legend (see \ref QCPLegend). + + The \a transformMode specifies whether the rescaling is done by a faster, low quality image + scaling algorithm (Qt::FastTransformation) or by a slower, higher quality algorithm + (Qt::SmoothTransformation). + + The current color map appearance is scaled down to \a thumbSize. Ideally, this should be equal to + the size of the legend icon (see \ref QCPLegend::setIconSize). If it isn't exactly the configured + legend icon size, the thumb will be rescaled during drawing of the legend item. + + \see setDataRange +*/ +void QCPColorMap::updateLegendIcon(Qt::TransformationMode transformMode, const QSize &thumbSize) +{ + if (mMapImage.isNull() && !data()->isEmpty()) + updateMapImage(); // try to update map image if it's null (happens if no draw has happened yet) + + if (!mMapImage.isNull()) // might still be null, e.g. if data is empty, so check here again + { + bool mirrorX = (keyAxis()->orientation() == Qt::Horizontal ? keyAxis() : valueAxis())->rangeReversed(); + bool mirrorY = (valueAxis()->orientation() == Qt::Vertical ? valueAxis() : keyAxis())->rangeReversed(); + mLegendIcon = QPixmap::fromImage(mMapImage.mirrored(mirrorX, mirrorY)).scaled(thumbSize, Qt::KeepAspectRatio, transformMode); + } +} + +/*! + Clears the colormap data by calling \ref QCPColorMapData::clear() on the internal data. This also + resizes the map to 0x0 cells. +*/ +void QCPColorMap::clearData() +{ + mMapData->clear(); +} + +/* inherits documentation from base class */ +double QCPColorMap::selectTest(const QPointF &pos, bool onlySelectable, QVariant *details) const +{ + Q_UNUSED(details) + if (onlySelectable && !mSelectable) + return -1; + if (!mKeyAxis || !mValueAxis) { qDebug() << Q_FUNC_INFO << "invalid key or value axis"; return -1; } + + if (mKeyAxis.data()->axisRect()->rect().contains(pos.toPoint())) + { + double posKey, posValue; + pixelsToCoords(pos, posKey, posValue); + if (mMapData->keyRange().contains(posKey) && mMapData->valueRange().contains(posValue)) + return mParentPlot->selectionTolerance()*0.99; + } + return -1; +} + +/*! \internal + + Updates the internal map image buffer by going through the internal \ref QCPColorMapData and + turning the data values into color pixels with \ref QCPColorGradient::colorize. + + This method is called by \ref QCPColorMap::draw if either the data has been modified or the map image + has been invalidated for a different reason (e.g. a change of the data range with \ref + setDataRange). + + If the map cell count is low, the image created will be oversampled in order to avoid a + QPainter::drawImage bug which makes inner pixel boundaries jitter when stretch-drawing images + without smooth transform enabled. Accordingly, oversampling isn't performed if \ref + setInterpolate is true. +*/ +void QCPColorMap::updateMapImage() +{ + QCPAxis *keyAxis = mKeyAxis.data(); + if (!keyAxis) return; + if (mMapData->isEmpty()) return; + + const int keySize = mMapData->keySize(); + const int valueSize = mMapData->valueSize(); + int keyOversamplingFactor = mInterpolate ? 1 : (int)(1.0+100.0/(double)keySize); // make mMapImage have at least size 100, factor becomes 1 if size > 200 or interpolation is on + int valueOversamplingFactor = mInterpolate ? 1 : (int)(1.0+100.0/(double)valueSize); // make mMapImage have at least size 100, factor becomes 1 if size > 200 or interpolation is on + + // resize mMapImage to correct dimensions including possible oversampling factors, according to key/value axes orientation: + if (keyAxis->orientation() == Qt::Horizontal && (mMapImage.width() != keySize*keyOversamplingFactor || mMapImage.height() != valueSize*valueOversamplingFactor)) + mMapImage = QImage(QSize(keySize*keyOversamplingFactor, valueSize*valueOversamplingFactor), QImage::Format_RGB32); + else if (keyAxis->orientation() == Qt::Vertical && (mMapImage.width() != valueSize*valueOversamplingFactor || mMapImage.height() != keySize*keyOversamplingFactor)) + mMapImage = QImage(QSize(valueSize*valueOversamplingFactor, keySize*keyOversamplingFactor), QImage::Format_RGB32); + + QImage *localMapImage = &mMapImage; // this is the image on which the colorization operates. Either the final mMapImage, or if we need oversampling, mUndersampledMapImage + if (keyOversamplingFactor > 1 || valueOversamplingFactor > 1) + { + // resize undersampled map image to actual key/value cell sizes: + if (keyAxis->orientation() == Qt::Horizontal && (mUndersampledMapImage.width() != keySize || mUndersampledMapImage.height() != valueSize)) + mUndersampledMapImage = QImage(QSize(keySize, valueSize), QImage::Format_RGB32); + else if (keyAxis->orientation() == Qt::Vertical && (mUndersampledMapImage.width() != valueSize || mUndersampledMapImage.height() != keySize)) + mUndersampledMapImage = QImage(QSize(valueSize, keySize), QImage::Format_RGB32); + localMapImage = &mUndersampledMapImage; // make the colorization run on the undersampled image + } else if (!mUndersampledMapImage.isNull()) + mUndersampledMapImage = QImage(); // don't need oversampling mechanism anymore (map size has changed) but mUndersampledMapImage still has nonzero size, free it + + const double *rawData = mMapData->mData; + if (keyAxis->orientation() == Qt::Horizontal) + { + const int lineCount = valueSize; + const int rowCount = keySize; + for (int line=0; line(localMapImage->scanLine(lineCount-1-line)); // invert scanline index because QImage counts scanlines from top, but our vertical index counts from bottom (mathematical coordinate system) + mGradient.colorize(rawData+line*rowCount, mDataRange, pixels, rowCount, 1, mDataScaleType==QCPAxis::stLogarithmic); + } + } else // keyAxis->orientation() == Qt::Vertical + { + const int lineCount = keySize; + const int rowCount = valueSize; + for (int line=0; line(localMapImage->scanLine(lineCount-1-line)); // invert scanline index because QImage counts scanlines from top, but our vertical index counts from bottom (mathematical coordinate system) + mGradient.colorize(rawData+line, mDataRange, pixels, rowCount, lineCount, mDataScaleType==QCPAxis::stLogarithmic); + } + } + + if (keyOversamplingFactor > 1 || valueOversamplingFactor > 1) + { + if (keyAxis->orientation() == Qt::Horizontal) + mMapImage = mUndersampledMapImage.scaled(keySize*keyOversamplingFactor, valueSize*valueOversamplingFactor, Qt::IgnoreAspectRatio, Qt::FastTransformation); + else + mMapImage = mUndersampledMapImage.scaled(valueSize*valueOversamplingFactor, keySize*keyOversamplingFactor, Qt::IgnoreAspectRatio, Qt::FastTransformation); + } + mMapData->mDataModified = false; + mMapImageInvalidated = false; +} + +/* inherits documentation from base class */ +void QCPColorMap::draw(QCPPainter *painter) +{ + if (mMapData->isEmpty()) return; + if (!mKeyAxis || !mValueAxis) return; + applyDefaultAntialiasingHint(painter); + + if (mMapData->mDataModified || mMapImageInvalidated) + updateMapImage(); + + // use buffer if painting vectorized (PDF): + bool useBuffer = painter->modes().testFlag(QCPPainter::pmVectorized); + QCPPainter *localPainter = painter; // will be redirected to paint on mapBuffer if painting vectorized + QRectF mapBufferTarget; // the rect in absolute widget coordinates where the visible map portion/buffer will end up in + QPixmap mapBuffer; + double mapBufferPixelRatio = 3; // factor by which DPI is increased in embedded bitmaps + if (useBuffer) + { + mapBufferTarget = painter->clipRegion().boundingRect(); + mapBuffer = QPixmap((mapBufferTarget.size()*mapBufferPixelRatio).toSize()); + mapBuffer.fill(Qt::transparent); + localPainter = new QCPPainter(&mapBuffer); + localPainter->scale(mapBufferPixelRatio, mapBufferPixelRatio); + localPainter->translate(-mapBufferTarget.topLeft()); + } + + QRectF imageRect = QRectF(coordsToPixels(mMapData->keyRange().lower, mMapData->valueRange().lower), + coordsToPixels(mMapData->keyRange().upper, mMapData->valueRange().upper)).normalized(); + // extend imageRect to contain outer halves/quarters of bordering/cornering pixels (cells are centered on map range boundary): + double halfCellWidth = 0; // in pixels + double halfCellHeight = 0; // in pixels + if (keyAxis()->orientation() == Qt::Horizontal) + { + if (mMapData->keySize() > 1) + halfCellWidth = 0.5*imageRect.width()/(double)(mMapData->keySize()-1); + if (mMapData->valueSize() > 1) + halfCellHeight = 0.5*imageRect.height()/(double)(mMapData->valueSize()-1); + } else // keyAxis orientation is Qt::Vertical + { + if (mMapData->keySize() > 1) + halfCellHeight = 0.5*imageRect.height()/(double)(mMapData->keySize()-1); + if (mMapData->valueSize() > 1) + halfCellWidth = 0.5*imageRect.width()/(double)(mMapData->valueSize()-1); + } + imageRect.adjust(-halfCellWidth, -halfCellHeight, halfCellWidth, halfCellHeight); + bool mirrorX = (keyAxis()->orientation() == Qt::Horizontal ? keyAxis() : valueAxis())->rangeReversed(); + bool mirrorY = (valueAxis()->orientation() == Qt::Vertical ? valueAxis() : keyAxis())->rangeReversed(); + bool smoothBackup = localPainter->renderHints().testFlag(QPainter::SmoothPixmapTransform); + localPainter->setRenderHint(QPainter::SmoothPixmapTransform, mInterpolate); + QRegion clipBackup; + if (mTightBoundary) + { + clipBackup = localPainter->clipRegion(); + QRectF tightClipRect = QRectF(coordsToPixels(mMapData->keyRange().lower, mMapData->valueRange().lower), + coordsToPixels(mMapData->keyRange().upper, mMapData->valueRange().upper)).normalized(); + localPainter->setClipRect(tightClipRect, Qt::IntersectClip); + } + localPainter->drawImage(imageRect, mMapImage.mirrored(mirrorX, mirrorY)); + if (mTightBoundary) + localPainter->setClipRegion(clipBackup); + localPainter->setRenderHint(QPainter::SmoothPixmapTransform, smoothBackup); + + if (useBuffer) // localPainter painted to mapBuffer, so now draw buffer with original painter + { + delete localPainter; + painter->drawPixmap(mapBufferTarget.toRect(), mapBuffer); + } +} + +/* inherits documentation from base class */ +void QCPColorMap::drawLegendIcon(QCPPainter *painter, const QRectF &rect) const +{ + applyDefaultAntialiasingHint(painter); + // draw map thumbnail: + if (!mLegendIcon.isNull()) + { + QPixmap scaledIcon = mLegendIcon.scaled(rect.size().toSize(), Qt::KeepAspectRatio, Qt::FastTransformation); + QRectF iconRect = QRectF(0, 0, scaledIcon.width(), scaledIcon.height()); + iconRect.moveCenter(rect.center()); + painter->drawPixmap(iconRect.topLeft(), scaledIcon); + } + /* + // draw frame: + painter->setBrush(Qt::NoBrush); + painter->setPen(Qt::black); + painter->drawRect(rect.adjusted(1, 1, 0, 0)); + */ +} + +/* inherits documentation from base class */ +QCPRange QCPColorMap::getKeyRange(bool &foundRange, SignDomain inSignDomain) const +{ + foundRange = true; + QCPRange result = mMapData->keyRange(); + result.normalize(); + if (inSignDomain == QCPAbstractPlottable::sdPositive) + { + if (result.lower <= 0 && result.upper > 0) + result.lower = result.upper*1e-3; + else if (result.lower <= 0 && result.upper <= 0) + foundRange = false; + } else if (inSignDomain == QCPAbstractPlottable::sdNegative) + { + if (result.upper >= 0 && result.lower < 0) + result.upper = result.lower*1e-3; + else if (result.upper >= 0 && result.lower >= 0) + foundRange = false; + } + return result; +} + +/* inherits documentation from base class */ +QCPRange QCPColorMap::getValueRange(bool &foundRange, SignDomain inSignDomain) const +{ + foundRange = true; + QCPRange result = mMapData->valueRange(); + result.normalize(); + if (inSignDomain == QCPAbstractPlottable::sdPositive) + { + if (result.lower <= 0 && result.upper > 0) + result.lower = result.upper*1e-3; + else if (result.lower <= 0 && result.upper <= 0) + foundRange = false; + } else if (inSignDomain == QCPAbstractPlottable::sdNegative) + { + if (result.upper >= 0 && result.lower < 0) + result.upper = result.lower*1e-3; + else if (result.upper >= 0 && result.lower >= 0) + foundRange = false; + } + return result; +} + + +//////////////////////////////////////////////////////////////////////////////////////////////////// +//////////////////// QCPFinancialData +//////////////////////////////////////////////////////////////////////////////////////////////////// + +/*! \class QCPFinancialData + \brief Holds the data of one single data point for QCPFinancial. + + The container for storing multiple data points is \ref QCPFinancialDataMap. + + The stored data is: + \li \a key: coordinate on the key axis of this data point + \li \a open: The opening value at the data point + \li \a high: The high/maximum value at the data point + \li \a low: The low/minimum value at the data point + \li \a close: The closing value at the data point + + \see QCPFinancialDataMap +*/ + +/*! + Constructs a data point with key and all values set to zero. +*/ +QCPFinancialData::QCPFinancialData() : + key(0), + open(0), + high(0), + low(0), + close(0) +{ +} + +/*! + Constructs a data point with the specified \a key and OHLC values. +*/ +QCPFinancialData::QCPFinancialData(double key, double open, double high, double low, double close) : + key(key), + open(open), + high(high), + low(low), + close(close) +{ +} + + +//////////////////////////////////////////////////////////////////////////////////////////////////// +//////////////////// QCPFinancial +//////////////////////////////////////////////////////////////////////////////////////////////////// + +/*! \class QCPFinancial + \brief A plottable representing a financial stock chart + + \image html QCPFinancial.png + + This plottable represents time series data binned to certain intervals, mainly used for stock + charts. The two common representations OHLC (Open-High-Low-Close) bars and Candlesticks can be + set via \ref setChartStyle. + + The data is passed via \ref setData as a set of open/high/low/close values at certain keys + (typically times). This means the data must be already binned appropriately. If data is only + available as a series of values (e.g. \a price against \a time), you can use the static + convenience function \ref timeSeriesToOhlc to generate binned OHLC-data which can then be passed + to \ref setData. + + The width of the OHLC bars/candlesticks can be controlled with \ref setWidth and is given in plot + key coordinates. A typical choice is to set it to (or slightly less than) one bin interval width. + + \section appearance Changing the appearance + + Charts can be either single- or two-colored (\ref setTwoColored). If set to be single-colored, + lines are drawn with the plottable's pen (\ref setPen) and fills with the brush (\ref setBrush). + + If set to two-colored, positive changes of the value during an interval (\a close >= \a open) are + represented with a different pen and brush than negative changes (\a close < \a open). These can + be configured with \ref setPenPositive, \ref setPenNegative, \ref setBrushPositive, and \ref + setBrushNegative. In two-colored mode, the normal plottable pen/brush is ignored. Upon selection + however, the normal selected pen/brush (\ref setSelectedPen, \ref setSelectedBrush) is used, + irrespective of whether the chart is single- or two-colored. + +*/ + +/* start of documentation of inline functions */ + +/*! \fn QCPFinancialDataMap *QCPFinancial::data() const + + Returns a pointer to the internal data storage of type \ref QCPFinancialDataMap. You may use it to + directly manipulate the data, which may be more convenient and faster than using the regular \ref + setData or \ref addData methods, in certain situations. +*/ + +/* end of documentation of inline functions */ + +/*! + Constructs a financial chart which uses \a keyAxis as its key axis ("x") and \a valueAxis as its value + axis ("y"). \a keyAxis and \a valueAxis must reside in the same QCustomPlot instance and not have + the same orientation. If either of these restrictions is violated, a corresponding message is + printed to the debug output (qDebug), the construction is not aborted, though. + + The constructed QCPFinancial can be added to the plot with QCustomPlot::addPlottable, QCustomPlot + then takes ownership of the financial chart. +*/ +QCPFinancial::QCPFinancial(QCPAxis *keyAxis, QCPAxis *valueAxis) : + QCPAbstractPlottable(keyAxis, valueAxis), + mData(0), + mChartStyle(csOhlc), + mWidth(0.5), + mTwoColored(false), + mBrushPositive(QBrush(QColor(210, 210, 255))), + mBrushNegative(QBrush(QColor(255, 210, 210))), + mPenPositive(QPen(QColor(10, 40, 180))), + mPenNegative(QPen(QColor(180, 40, 10))) +{ + mData = new QCPFinancialDataMap; + + setSelectedPen(QPen(QColor(80, 80, 255), 2.5)); + setSelectedBrush(QBrush(QColor(80, 80, 255))); +} + +QCPFinancial::~QCPFinancial() +{ + delete mData; +} + +/*! + Replaces the current data with the provided \a data. + + If \a copy is set to true, data points in \a data will only be copied. if false, the plottable + takes ownership of the passed data and replaces the internal data pointer with it. This is + significantly faster than copying for large datasets. + + Alternatively, you can also access and modify the plottable's data via the \ref data method, which + returns a pointer to the internal \ref QCPFinancialDataMap. + + \see timeSeriesToOhlc +*/ +void QCPFinancial::setData(QCPFinancialDataMap *data, bool copy) +{ + if (mData == data) + { + qDebug() << Q_FUNC_INFO << "The data pointer is already in (and owned by) this plottable" << reinterpret_cast(data); + return; + } + if (copy) + { + *mData = *data; + } else + { + delete mData; + mData = data; + } +} + +/*! \overload + + Replaces the current data with the provided open/high/low/close data. The provided vectors should + have equal length. Else, the number of added points will be the size of the smallest vector. + + \see timeSeriesToOhlc +*/ +void QCPFinancial::setData(const QVector &key, const QVector &open, const QVector &high, const QVector &low, const QVector &close) +{ + mData->clear(); + int n = key.size(); + n = qMin(n, open.size()); + n = qMin(n, high.size()); + n = qMin(n, low.size()); + n = qMin(n, close.size()); + for (int i=0; iinsertMulti(key[i], QCPFinancialData(key[i], open[i], high[i], low[i], close[i])); + } +} + +/*! + Sets which representation style shall be used to display the OHLC data. +*/ +void QCPFinancial::setChartStyle(QCPFinancial::ChartStyle style) +{ + mChartStyle = style; +} + +/*! + Sets the width of the individual bars/candlesticks to \a width in plot key coordinates. + + A typical choice is to set it to (or slightly less than) one bin interval width. +*/ +void QCPFinancial::setWidth(double width) +{ + mWidth = width; +} + +/*! + Sets whether this chart shall contrast positive from negative trends per data point by using two + separate colors to draw the respective bars/candlesticks. + + If \a twoColored is false, the normal plottable's pen and brush are used (\ref setPen, \ref + setBrush). + + \see setPenPositive, setPenNegative, setBrushPositive, setBrushNegative +*/ +void QCPFinancial::setTwoColored(bool twoColored) +{ + mTwoColored = twoColored; +} + +/*! + If \ref setTwoColored is set to true, this function controls the brush that is used to draw fills + of data points with a positive trend (i.e. bars/candlesticks with close >= open). + + If \a twoColored is false, the normal plottable's pen and brush are used (\ref setPen, \ref + setBrush). + + \see setBrushNegative, setPenPositive, setPenNegative +*/ +void QCPFinancial::setBrushPositive(const QBrush &brush) +{ + mBrushPositive = brush; +} + +/*! + If \ref setTwoColored is set to true, this function controls the brush that is used to draw fills + of data points with a negative trend (i.e. bars/candlesticks with close < open). + + If \a twoColored is false, the normal plottable's pen and brush are used (\ref setPen, \ref + setBrush). + + \see setBrushPositive, setPenNegative, setPenPositive +*/ +void QCPFinancial::setBrushNegative(const QBrush &brush) +{ + mBrushNegative = brush; +} + +/*! + If \ref setTwoColored is set to true, this function controls the pen that is used to draw + outlines of data points with a positive trend (i.e. bars/candlesticks with close >= open). + + If \a twoColored is false, the normal plottable's pen and brush are used (\ref setPen, \ref + setBrush). + + \see setPenNegative, setBrushPositive, setBrushNegative +*/ +void QCPFinancial::setPenPositive(const QPen &pen) +{ + mPenPositive = pen; +} + +/*! + If \ref setTwoColored is set to true, this function controls the pen that is used to draw + outlines of data points with a negative trend (i.e. bars/candlesticks with close < open). + + If \a twoColored is false, the normal plottable's pen and brush are used (\ref setPen, \ref + setBrush). + + \see setPenPositive, setBrushNegative, setBrushPositive +*/ +void QCPFinancial::setPenNegative(const QPen &pen) +{ + mPenNegative = pen; +} + +/*! + Adds the provided data points in \a dataMap to the current data. + + Alternatively, you can also access and modify the data via the \ref data method, which returns a + pointer to the internal \ref QCPFinancialDataMap. + + \see removeData +*/ +void QCPFinancial::addData(const QCPFinancialDataMap &dataMap) +{ + mData->unite(dataMap); +} + +/*! \overload + + Adds the provided single data point in \a data to the current data. + + Alternatively, you can also access and modify the data via the \ref data method, which returns a + pointer to the internal \ref QCPFinancialData. + + \see removeData +*/ +void QCPFinancial::addData(const QCPFinancialData &data) +{ + mData->insertMulti(data.key, data); +} + +/*! \overload + + Adds the provided single data point given by \a key, \a open, \a high, \a low, and \a close to + the current data. + + Alternatively, you can also access and modify the data via the \ref data method, which returns a + pointer to the internal \ref QCPFinancialData. + + \see removeData +*/ +void QCPFinancial::addData(double key, double open, double high, double low, double close) +{ + mData->insertMulti(key, QCPFinancialData(key, open, high, low, close)); +} + +/*! \overload + + Adds the provided open/high/low/close data to the current data. + + Alternatively, you can also access and modify the data via the \ref data method, which returns a + pointer to the internal \ref QCPFinancialData. + + \see removeData +*/ +void QCPFinancial::addData(const QVector &key, const QVector &open, const QVector &high, const QVector &low, const QVector &close) +{ + int n = key.size(); + n = qMin(n, open.size()); + n = qMin(n, high.size()); + n = qMin(n, low.size()); + n = qMin(n, close.size()); + for (int i=0; iinsertMulti(key[i], QCPFinancialData(key[i], open[i], high[i], low[i], close[i])); + } +} + +/*! + Removes all data points with keys smaller than \a key. + + \see addData, clearData +*/ +void QCPFinancial::removeDataBefore(double key) +{ + QCPFinancialDataMap::iterator it = mData->begin(); + while (it != mData->end() && it.key() < key) + it = mData->erase(it); +} + +/*! + Removes all data points with keys greater than \a key. + + \see addData, clearData +*/ +void QCPFinancial::removeDataAfter(double key) +{ + if (mData->isEmpty()) return; + QCPFinancialDataMap::iterator it = mData->upperBound(key); + while (it != mData->end()) + it = mData->erase(it); +} + +/*! + Removes all data points with keys between \a fromKey and \a toKey. if \a fromKey is greater or + equal to \a toKey, the function does nothing. To remove a single data point with known key, use + \ref removeData(double key). + + \see addData, clearData +*/ +void QCPFinancial::removeData(double fromKey, double toKey) +{ + if (fromKey >= toKey || mData->isEmpty()) return; + QCPFinancialDataMap::iterator it = mData->upperBound(fromKey); + QCPFinancialDataMap::iterator itEnd = mData->upperBound(toKey); + while (it != itEnd) + it = mData->erase(it); +} + +/*! \overload + + Removes a single data point at \a key. If the position is not known with absolute precision, + consider using \ref removeData(double fromKey, double toKey) with a small fuzziness interval + around the suspected position, depeding on the precision with which the key is known. + + \see addData, clearData +*/ +void QCPFinancial::removeData(double key) +{ + mData->remove(key); +} + +/*! + Removes all data points. + + \see removeData, removeDataAfter, removeDataBefore +*/ +void QCPFinancial::clearData() +{ + mData->clear(); +} + +/* inherits documentation from base class */ +double QCPFinancial::selectTest(const QPointF &pos, bool onlySelectable, QVariant *details) const +{ + Q_UNUSED(details) + if (onlySelectable && !mSelectable) + return -1; + if (!mKeyAxis || !mValueAxis) { qDebug() << Q_FUNC_INFO << "invalid key or value axis"; return -1; } + + if (mKeyAxis.data()->axisRect()->rect().contains(pos.toPoint())) + { + // get visible data range: + QCPFinancialDataMap::const_iterator lower, upper; // note that upper is the actual upper point, and not 1 step after the upper point + getVisibleDataBounds(lower, upper); + if (lower == mData->constEnd() || upper == mData->constEnd()) + return -1; + // perform select test according to configured style: + switch (mChartStyle) + { + case QCPFinancial::csOhlc: + return ohlcSelectTest(pos, lower, upper+1); break; + case QCPFinancial::csCandlestick: + return candlestickSelectTest(pos, lower, upper+1); break; + } + } + return -1; +} + +/*! + A convenience function that converts time series data (\a value against \a time) to OHLC binned + data points. The return value can then be passed on to \ref setData. + + The size of the bins can be controlled with \a timeBinSize in the same units as \a time is given. + For example, if the unit of \a time is seconds and single OHLC/Candlesticks should span an hour + each, set \a timeBinSize to 3600. + + \a timeBinOffset allows to control precisely at what \a time coordinate a bin should start. The + value passed as \a timeBinOffset doesn't need to be in the range encompassed by the \a time keys. + It merely defines the mathematical offset/phase of the bins that will be used to process the + data. +*/ +QCPFinancialDataMap QCPFinancial::timeSeriesToOhlc(const QVector &time, const QVector &value, double timeBinSize, double timeBinOffset) +{ + QCPFinancialDataMap map; + int count = qMin(time.size(), value.size()); + if (count == 0) + return QCPFinancialDataMap(); + + QCPFinancialData currentBinData(0, value.first(), value.first(), value.first(), value.first()); + int currentBinIndex = qFloor((time.first()-timeBinOffset)/timeBinSize+0.5); + for (int i=0; i currentBinData.high) currentBinData.high = value.at(i); + if (i == count-1) // last data point is in current bin, finalize bin: + { + currentBinData.close = value.at(i); + currentBinData.key = timeBinOffset+(index)*timeBinSize; + map.insert(currentBinData.key, currentBinData); + } + } else // data point not anymore in current bin, set close of old and open of new bin, and add old to map: + { + // finalize current bin: + currentBinData.close = value.at(i-1); + currentBinData.key = timeBinOffset+(index-1)*timeBinSize; + map.insert(currentBinData.key, currentBinData); + // start next bin: + currentBinIndex = index; + currentBinData.open = value.at(i); + currentBinData.high = value.at(i); + currentBinData.low = value.at(i); + } + } + + return map; +} + +/* inherits documentation from base class */ +void QCPFinancial::draw(QCPPainter *painter) +{ + // get visible data range: + QCPFinancialDataMap::const_iterator lower, upper; // note that upper is the actual upper point, and not 1 step after the upper point + getVisibleDataBounds(lower, upper); + if (lower == mData->constEnd() || upper == mData->constEnd()) + return; + + // draw visible data range according to configured style: + switch (mChartStyle) + { + case QCPFinancial::csOhlc: + drawOhlcPlot(painter, lower, upper+1); break; + case QCPFinancial::csCandlestick: + drawCandlestickPlot(painter, lower, upper+1); break; + } +} + +/* inherits documentation from base class */ +void QCPFinancial::drawLegendIcon(QCPPainter *painter, const QRectF &rect) const +{ + painter->setAntialiasing(false); // legend icon especially of csCandlestick looks better without antialiasing + if (mChartStyle == csOhlc) + { + if (mTwoColored) + { + // draw upper left half icon with positive color: + painter->setBrush(mBrushPositive); + painter->setPen(mPenPositive); + painter->setClipRegion(QRegion(QPolygon() << rect.bottomLeft().toPoint() << rect.topRight().toPoint() << rect.topLeft().toPoint())); + painter->drawLine(QLineF(0, rect.height()*0.5, rect.width(), rect.height()*0.5).translated(rect.topLeft())); + painter->drawLine(QLineF(rect.width()*0.2, rect.height()*0.3, rect.width()*0.2, rect.height()*0.5).translated(rect.topLeft())); + painter->drawLine(QLineF(rect.width()*0.8, rect.height()*0.5, rect.width()*0.8, rect.height()*0.7).translated(rect.topLeft())); + // draw bottom right hald icon with negative color: + painter->setBrush(mBrushNegative); + painter->setPen(mPenNegative); + painter->setClipRegion(QRegion(QPolygon() << rect.bottomLeft().toPoint() << rect.topRight().toPoint() << rect.bottomRight().toPoint())); + painter->drawLine(QLineF(0, rect.height()*0.5, rect.width(), rect.height()*0.5).translated(rect.topLeft())); + painter->drawLine(QLineF(rect.width()*0.2, rect.height()*0.3, rect.width()*0.2, rect.height()*0.5).translated(rect.topLeft())); + painter->drawLine(QLineF(rect.width()*0.8, rect.height()*0.5, rect.width()*0.8, rect.height()*0.7).translated(rect.topLeft())); + } else + { + painter->setBrush(mBrush); + painter->setPen(mPen); + painter->drawLine(QLineF(0, rect.height()*0.5, rect.width(), rect.height()*0.5).translated(rect.topLeft())); + painter->drawLine(QLineF(rect.width()*0.2, rect.height()*0.3, rect.width()*0.2, rect.height()*0.5).translated(rect.topLeft())); + painter->drawLine(QLineF(rect.width()*0.8, rect.height()*0.5, rect.width()*0.8, rect.height()*0.7).translated(rect.topLeft())); + } + } else if (mChartStyle == csCandlestick) + { + if (mTwoColored) + { + // draw upper left half icon with positive color: + painter->setBrush(mBrushPositive); + painter->setPen(mPenPositive); + painter->setClipRegion(QRegion(QPolygon() << rect.bottomLeft().toPoint() << rect.topRight().toPoint() << rect.topLeft().toPoint())); + painter->drawLine(QLineF(0, rect.height()*0.5, rect.width()*0.25, rect.height()*0.5).translated(rect.topLeft())); + painter->drawLine(QLineF(rect.width()*0.75, rect.height()*0.5, rect.width(), rect.height()*0.5).translated(rect.topLeft())); + painter->drawRect(QRectF(rect.width()*0.25, rect.height()*0.25, rect.width()*0.5, rect.height()*0.5).translated(rect.topLeft())); + // draw bottom right hald icon with negative color: + painter->setBrush(mBrushNegative); + painter->setPen(mPenNegative); + painter->setClipRegion(QRegion(QPolygon() << rect.bottomLeft().toPoint() << rect.topRight().toPoint() << rect.bottomRight().toPoint())); + painter->drawLine(QLineF(0, rect.height()*0.5, rect.width()*0.25, rect.height()*0.5).translated(rect.topLeft())); + painter->drawLine(QLineF(rect.width()*0.75, rect.height()*0.5, rect.width(), rect.height()*0.5).translated(rect.topLeft())); + painter->drawRect(QRectF(rect.width()*0.25, rect.height()*0.25, rect.width()*0.5, rect.height()*0.5).translated(rect.topLeft())); + } else + { + painter->setBrush(mBrush); + painter->setPen(mPen); + painter->drawLine(QLineF(0, rect.height()*0.5, rect.width()*0.25, rect.height()*0.5).translated(rect.topLeft())); + painter->drawLine(QLineF(rect.width()*0.75, rect.height()*0.5, rect.width(), rect.height()*0.5).translated(rect.topLeft())); + painter->drawRect(QRectF(rect.width()*0.25, rect.height()*0.25, rect.width()*0.5, rect.height()*0.5).translated(rect.topLeft())); + } + } +} + +/* inherits documentation from base class */ +QCPRange QCPFinancial::getKeyRange(bool &foundRange, QCPAbstractPlottable::SignDomain inSignDomain) const +{ + QCPRange range; + bool haveLower = false; + bool haveUpper = false; + + double current; + QCPFinancialDataMap::const_iterator it = mData->constBegin(); + while (it != mData->constEnd()) + { + current = it.value().key; + if (inSignDomain == sdBoth || (inSignDomain == sdNegative && current < 0) || (inSignDomain == sdPositive && current > 0)) + { + if (current < range.lower || !haveLower) + { + range.lower = current; + haveLower = true; + } + if (current > range.upper || !haveUpper) + { + range.upper = current; + haveUpper = true; + } + } + ++it; + } + // determine exact range by including width of bars/flags: + if (haveLower && mKeyAxis) + range.lower = range.lower-mWidth*0.5; + if (haveUpper && mKeyAxis) + range.upper = range.upper+mWidth*0.5; + foundRange = haveLower && haveUpper; + return range; +} + +/* inherits documentation from base class */ +QCPRange QCPFinancial::getValueRange(bool &foundRange, QCPAbstractPlottable::SignDomain inSignDomain) const +{ + QCPRange range; + bool haveLower = false; + bool haveUpper = false; + + QCPFinancialDataMap::const_iterator it = mData->constBegin(); + while (it != mData->constEnd()) + { + // high: + if (inSignDomain == sdBoth || (inSignDomain == sdNegative && it.value().high < 0) || (inSignDomain == sdPositive && it.value().high > 0)) + { + if (it.value().high < range.lower || !haveLower) + { + range.lower = it.value().high; + haveLower = true; + } + if (it.value().high > range.upper || !haveUpper) + { + range.upper = it.value().high; + haveUpper = true; + } + } + // low: + if (inSignDomain == sdBoth || (inSignDomain == sdNegative && it.value().low < 0) || (inSignDomain == sdPositive && it.value().low > 0)) + { + if (it.value().low < range.lower || !haveLower) + { + range.lower = it.value().low; + haveLower = true; + } + if (it.value().low > range.upper || !haveUpper) + { + range.upper = it.value().low; + haveUpper = true; + } + } + ++it; + } + + foundRange = haveLower && haveUpper; + return range; +} + +/*! \internal + + Draws the data from \a begin to \a end as OHLC bars with the provided \a painter. + + This method is a helper function for \ref draw. It is used when the chart style is \ref csOhlc. +*/ +void QCPFinancial::drawOhlcPlot(QCPPainter *painter, const QCPFinancialDataMap::const_iterator &begin, const QCPFinancialDataMap::const_iterator &end) +{ + QCPAxis *keyAxis = mKeyAxis.data(); + QCPAxis *valueAxis = mValueAxis.data(); + if (!keyAxis || !valueAxis) { qDebug() << Q_FUNC_INFO << "invalid key or value axis"; return; } + + QPen linePen; + + if (keyAxis->orientation() == Qt::Horizontal) + { + for (QCPFinancialDataMap::const_iterator it = begin; it != end; ++it) + { + if (mSelected) + linePen = mSelectedPen; + else if (mTwoColored) + linePen = it.value().close >= it.value().open ? mPenPositive : mPenNegative; + else + linePen = mPen; + painter->setPen(linePen); + double keyPixel = keyAxis->coordToPixel(it.value().key); + double openPixel = valueAxis->coordToPixel(it.value().open); + double closePixel = valueAxis->coordToPixel(it.value().close); + // draw backbone: + painter->drawLine(QPointF(keyPixel, valueAxis->coordToPixel(it.value().high)), QPointF(keyPixel, valueAxis->coordToPixel(it.value().low))); + // draw open: + double keyWidthPixels = keyPixel-keyAxis->coordToPixel(it.value().key-mWidth*0.5); // sign of this makes sure open/close are on correct sides + painter->drawLine(QPointF(keyPixel-keyWidthPixels, openPixel), QPointF(keyPixel, openPixel)); + // draw close: + painter->drawLine(QPointF(keyPixel, closePixel), QPointF(keyPixel+keyWidthPixels, closePixel)); + } + } else + { + for (QCPFinancialDataMap::const_iterator it = begin; it != end; ++it) + { + if (mSelected) + linePen = mSelectedPen; + else if (mTwoColored) + linePen = it.value().close >= it.value().open ? mPenPositive : mPenNegative; + else + linePen = mPen; + painter->setPen(linePen); + double keyPixel = keyAxis->coordToPixel(it.value().key); + double openPixel = valueAxis->coordToPixel(it.value().open); + double closePixel = valueAxis->coordToPixel(it.value().close); + // draw backbone: + painter->drawLine(QPointF(valueAxis->coordToPixel(it.value().high), keyPixel), QPointF(valueAxis->coordToPixel(it.value().low), keyPixel)); + // draw open: + double keyWidthPixels = keyPixel-keyAxis->coordToPixel(it.value().key-mWidth*0.5); // sign of this makes sure open/close are on correct sides + painter->drawLine(QPointF(openPixel, keyPixel-keyWidthPixels), QPointF(openPixel, keyPixel)); + // draw close: + painter->drawLine(QPointF(closePixel, keyPixel), QPointF(closePixel, keyPixel+keyWidthPixels)); + } + } +} + +/*! \internal + + Draws the data from \a begin to \a end as Candlesticks with the provided \a painter. + + This method is a helper function for \ref draw. It is used when the chart style is \ref csCandlestick. +*/ +void QCPFinancial::drawCandlestickPlot(QCPPainter *painter, const QCPFinancialDataMap::const_iterator &begin, const QCPFinancialDataMap::const_iterator &end) +{ + QCPAxis *keyAxis = mKeyAxis.data(); + QCPAxis *valueAxis = mValueAxis.data(); + if (!keyAxis || !valueAxis) { qDebug() << Q_FUNC_INFO << "invalid key or value axis"; return; } + + QPen linePen; + QBrush boxBrush; + + if (keyAxis->orientation() == Qt::Horizontal) + { + for (QCPFinancialDataMap::const_iterator it = begin; it != end; ++it) + { + if (mSelected) + { + linePen = mSelectedPen; + boxBrush = mSelectedBrush; + } else if (mTwoColored) + { + if (it.value().close >= it.value().open) + { + linePen = mPenPositive; + boxBrush = mBrushPositive; + } else + { + linePen = mPenNegative; + boxBrush = mBrushNegative; + } + } else + { + linePen = mPen; + boxBrush = mBrush; + } + painter->setPen(linePen); + painter->setBrush(boxBrush); + double keyPixel = keyAxis->coordToPixel(it.value().key); + double openPixel = valueAxis->coordToPixel(it.value().open); + double closePixel = valueAxis->coordToPixel(it.value().close); + // draw high: + painter->drawLine(QPointF(keyPixel, valueAxis->coordToPixel(it.value().high)), QPointF(keyPixel, valueAxis->coordToPixel(qMax(it.value().open, it.value().close)))); + // draw low: + painter->drawLine(QPointF(keyPixel, valueAxis->coordToPixel(it.value().low)), QPointF(keyPixel, valueAxis->coordToPixel(qMin(it.value().open, it.value().close)))); + // draw open-close box: + double keyWidthPixels = keyPixel-keyAxis->coordToPixel(it.value().key-mWidth*0.5); + painter->drawRect(QRectF(QPointF(keyPixel-keyWidthPixels, closePixel), QPointF(keyPixel+keyWidthPixels, openPixel))); + } + } else // keyAxis->orientation() == Qt::Vertical + { + for (QCPFinancialDataMap::const_iterator it = begin; it != end; ++it) + { + if (mSelected) + { + linePen = mSelectedPen; + boxBrush = mSelectedBrush; + } else if (mTwoColored) + { + if (it.value().close >= it.value().open) + { + linePen = mPenPositive; + boxBrush = mBrushPositive; + } else + { + linePen = mPenNegative; + boxBrush = mBrushNegative; + } + } else + { + linePen = mPen; + boxBrush = mBrush; + } + painter->setPen(linePen); + painter->setBrush(boxBrush); + double keyPixel = keyAxis->coordToPixel(it.value().key); + double openPixel = valueAxis->coordToPixel(it.value().open); + double closePixel = valueAxis->coordToPixel(it.value().close); + // draw high: + painter->drawLine(QPointF(valueAxis->coordToPixel(it.value().high), keyPixel), QPointF(valueAxis->coordToPixel(qMax(it.value().open, it.value().close)), keyPixel)); + // draw low: + painter->drawLine(QPointF(valueAxis->coordToPixel(it.value().low), keyPixel), QPointF(valueAxis->coordToPixel(qMin(it.value().open, it.value().close)), keyPixel)); + // draw open-close box: + double keyWidthPixels = keyPixel-keyAxis->coordToPixel(it.value().key-mWidth*0.5); + painter->drawRect(QRectF(QPointF(closePixel, keyPixel-keyWidthPixels), QPointF(openPixel, keyPixel+keyWidthPixels))); + } + } +} + +/*! \internal + + This method is a helper function for \ref selectTest. It is used to test for selection when the + chart style is \ref csOhlc. It only tests against the data points between \a begin and \a end. +*/ +double QCPFinancial::ohlcSelectTest(const QPointF &pos, const QCPFinancialDataMap::const_iterator &begin, const QCPFinancialDataMap::const_iterator &end) const +{ + QCPAxis *keyAxis = mKeyAxis.data(); + QCPAxis *valueAxis = mValueAxis.data(); + if (!keyAxis || !valueAxis) { qDebug() << Q_FUNC_INFO << "invalid key or value axis"; return -1; } + + double minDistSqr = std::numeric_limits::max(); + QCPFinancialDataMap::const_iterator it; + if (keyAxis->orientation() == Qt::Horizontal) + { + for (it = begin; it != end; ++it) + { + double keyPixel = keyAxis->coordToPixel(it.value().key); + // calculate distance to backbone: + double currentDistSqr = distSqrToLine(QPointF(keyPixel, valueAxis->coordToPixel(it.value().high)), QPointF(keyPixel, valueAxis->coordToPixel(it.value().low)), pos); + if (currentDistSqr < minDistSqr) + minDistSqr = currentDistSqr; + } + } else // keyAxis->orientation() == Qt::Vertical + { + for (it = begin; it != end; ++it) + { + double keyPixel = keyAxis->coordToPixel(it.value().key); + // calculate distance to backbone: + double currentDistSqr = distSqrToLine(QPointF(valueAxis->coordToPixel(it.value().high), keyPixel), QPointF(valueAxis->coordToPixel(it.value().low), keyPixel), pos); + if (currentDistSqr < minDistSqr) + minDistSqr = currentDistSqr; + } + } + return qSqrt(minDistSqr); +} + +/*! \internal + + This method is a helper function for \ref selectTest. It is used to test for selection when the + chart style is \ref csCandlestick. It only tests against the data points between \a begin and \a + end. +*/ +double QCPFinancial::candlestickSelectTest(const QPointF &pos, const QCPFinancialDataMap::const_iterator &begin, const QCPFinancialDataMap::const_iterator &end) const +{ + QCPAxis *keyAxis = mKeyAxis.data(); + QCPAxis *valueAxis = mValueAxis.data(); + if (!keyAxis || !valueAxis) { qDebug() << Q_FUNC_INFO << "invalid key or value axis"; return -1; } + + double minDistSqr = std::numeric_limits::max(); + QCPFinancialDataMap::const_iterator it; + if (keyAxis->orientation() == Qt::Horizontal) + { + for (it = begin; it != end; ++it) + { + double currentDistSqr; + // determine whether pos is in open-close-box: + QCPRange boxKeyRange(it.value().key-mWidth*0.5, it.value().key+mWidth*0.5); + QCPRange boxValueRange(it.value().close, it.value().open); + double posKey, posValue; + pixelsToCoords(pos, posKey, posValue); + if (boxKeyRange.contains(posKey) && boxValueRange.contains(posValue)) // is in open-close-box + { + currentDistSqr = mParentPlot->selectionTolerance()*0.99 * mParentPlot->selectionTolerance()*0.99; + } else + { + // calculate distance to high/low lines: + double keyPixel = keyAxis->coordToPixel(it.value().key); + double highLineDistSqr = distSqrToLine(QPointF(keyPixel, valueAxis->coordToPixel(it.value().high)), QPointF(keyPixel, valueAxis->coordToPixel(qMax(it.value().open, it.value().close))), pos); + double lowLineDistSqr = distSqrToLine(QPointF(keyPixel, valueAxis->coordToPixel(it.value().low)), QPointF(keyPixel, valueAxis->coordToPixel(qMin(it.value().open, it.value().close))), pos); + currentDistSqr = qMin(highLineDistSqr, lowLineDistSqr); + } + if (currentDistSqr < minDistSqr) + minDistSqr = currentDistSqr; + } + } else // keyAxis->orientation() == Qt::Vertical + { + for (it = begin; it != end; ++it) + { + double currentDistSqr; + // determine whether pos is in open-close-box: + QCPRange boxKeyRange(it.value().key-mWidth*0.5, it.value().key+mWidth*0.5); + QCPRange boxValueRange(it.value().close, it.value().open); + double posKey, posValue; + pixelsToCoords(pos, posKey, posValue); + if (boxKeyRange.contains(posKey) && boxValueRange.contains(posValue)) // is in open-close-box + { + currentDistSqr = mParentPlot->selectionTolerance()*0.99 * mParentPlot->selectionTolerance()*0.99; + } else + { + // calculate distance to high/low lines: + double keyPixel = keyAxis->coordToPixel(it.value().key); + double highLineDistSqr = distSqrToLine(QPointF(valueAxis->coordToPixel(it.value().high), keyPixel), QPointF(valueAxis->coordToPixel(qMax(it.value().open, it.value().close)), keyPixel), pos); + double lowLineDistSqr = distSqrToLine(QPointF(valueAxis->coordToPixel(it.value().low), keyPixel), QPointF(valueAxis->coordToPixel(qMin(it.value().open, it.value().close)), keyPixel), pos); + currentDistSqr = qMin(highLineDistSqr, lowLineDistSqr); + } + if (currentDistSqr < minDistSqr) + minDistSqr = currentDistSqr; + } + } + return qSqrt(minDistSqr); +} + +/*! \internal + + called by the drawing methods to determine which data (key) range is visible at the current key + axis range setting, so only that needs to be processed. + + \a lower returns an iterator to the lowest data point that needs to be taken into account when + plotting. Note that in order to get a clean plot all the way to the edge of the axis rect, \a + lower may still be just outside the visible range. + + \a upper returns an iterator to the highest data point. Same as before, \a upper may also lie + just outside of the visible range. + + if the plottable contains no data, both \a lower and \a upper point to constEnd. + + \see QCPGraph::getVisibleDataBounds +*/ +void QCPFinancial::getVisibleDataBounds(QCPFinancialDataMap::const_iterator &lower, QCPFinancialDataMap::const_iterator &upper) const +{ + if (!mKeyAxis) { qDebug() << Q_FUNC_INFO << "invalid key axis"; return; } + if (mData->isEmpty()) + { + lower = mData->constEnd(); + upper = mData->constEnd(); + return; + } + + // get visible data range as QMap iterators + QCPFinancialDataMap::const_iterator lbound = mData->lowerBound(mKeyAxis.data()->range().lower); + QCPFinancialDataMap::const_iterator ubound = mData->upperBound(mKeyAxis.data()->range().upper); + bool lowoutlier = lbound != mData->constBegin(); // indicates whether there exist points below axis range + bool highoutlier = ubound != mData->constEnd(); // indicates whether there exist points above axis range + + lower = (lowoutlier ? lbound-1 : lbound); // data point range that will be actually drawn + upper = (highoutlier ? ubound : ubound-1); // data point range that will be actually drawn +} + + +//////////////////////////////////////////////////////////////////////////////////////////////////// +//////////////////// QCPItemStraightLine +//////////////////////////////////////////////////////////////////////////////////////////////////// + +/*! \class QCPItemStraightLine + \brief A straight line that spans infinitely in both directions + + \image html QCPItemStraightLine.png "Straight line example. Blue dotted circles are anchors, solid blue discs are positions." + + It has two positions, \a point1 and \a point2, which define the straight line. +*/ + +/*! + Creates a straight line item and sets default values. + + The constructed item can be added to the plot with QCustomPlot::addItem. +*/ +QCPItemStraightLine::QCPItemStraightLine(QCustomPlot *parentPlot) : + QCPAbstractItem(parentPlot), + point1(createPosition(QLatin1String("point1"))), + point2(createPosition(QLatin1String("point2"))) +{ + point1->setCoords(0, 0); + point2->setCoords(1, 1); + + setPen(QPen(Qt::black)); + setSelectedPen(QPen(Qt::blue,2)); +} + +QCPItemStraightLine::~QCPItemStraightLine() +{ +} + +/*! + Sets the pen that will be used to draw the line + + \see setSelectedPen +*/ +void QCPItemStraightLine::setPen(const QPen &pen) +{ + mPen = pen; +} + +/*! + Sets the pen that will be used to draw the line when selected + + \see setPen, setSelected +*/ +void QCPItemStraightLine::setSelectedPen(const QPen &pen) +{ + mSelectedPen = pen; +} + +/* inherits documentation from base class */ +double QCPItemStraightLine::selectTest(const QPointF &pos, bool onlySelectable, QVariant *details) const +{ + Q_UNUSED(details) + if (onlySelectable && !mSelectable) + return -1; + + return distToStraightLine(QVector2D(point1->pixelPoint()), QVector2D(point2->pixelPoint()-point1->pixelPoint()), QVector2D(pos)); +} + +/* inherits documentation from base class */ +void QCPItemStraightLine::draw(QCPPainter *painter) +{ + QVector2D start(point1->pixelPoint()); + QVector2D end(point2->pixelPoint()); + // get visible segment of straight line inside clipRect: + double clipPad = mainPen().widthF(); + QLineF line = getRectClippedStraightLine(start, end-start, clipRect().adjusted(-clipPad, -clipPad, clipPad, clipPad)); + // paint visible segment, if existent: + if (!line.isNull()) + { + painter->setPen(mainPen()); + painter->drawLine(line); + } +} + +/*! \internal + + finds the shortest distance of \a point to the straight line defined by the base point \a + base and the direction vector \a vec. + + This is a helper function for \ref selectTest. +*/ +double QCPItemStraightLine::distToStraightLine(const QVector2D &base, const QVector2D &vec, const QVector2D &point) const +{ + return qAbs((base.y()-point.y())*vec.x()-(base.x()-point.x())*vec.y())/vec.length(); +} + +/*! \internal + + Returns the section of the straight line defined by \a base and direction vector \a + vec, that is visible in the specified \a rect. + + This is a helper function for \ref draw. +*/ +QLineF QCPItemStraightLine::getRectClippedStraightLine(const QVector2D &base, const QVector2D &vec, const QRect &rect) const +{ + double bx, by; + double gamma; + QLineF result; + if (vec.x() == 0 && vec.y() == 0) + return result; + if (qFuzzyIsNull(vec.x())) // line is vertical + { + // check top of rect: + bx = rect.left(); + by = rect.top(); + gamma = base.x()-bx + (by-base.y())*vec.x()/vec.y(); + if (gamma >= 0 && gamma <= rect.width()) + result.setLine(bx+gamma, rect.top(), bx+gamma, rect.bottom()); // no need to check bottom because we know line is vertical + } else if (qFuzzyIsNull(vec.y())) // line is horizontal + { + // check left of rect: + bx = rect.left(); + by = rect.top(); + gamma = base.y()-by + (bx-base.x())*vec.y()/vec.x(); + if (gamma >= 0 && gamma <= rect.height()) + result.setLine(rect.left(), by+gamma, rect.right(), by+gamma); // no need to check right because we know line is horizontal + } else // line is skewed + { + QList pointVectors; + // check top of rect: + bx = rect.left(); + by = rect.top(); + gamma = base.x()-bx + (by-base.y())*vec.x()/vec.y(); + if (gamma >= 0 && gamma <= rect.width()) + pointVectors.append(QVector2D(bx+gamma, by)); + // check bottom of rect: + bx = rect.left(); + by = rect.bottom(); + gamma = base.x()-bx + (by-base.y())*vec.x()/vec.y(); + if (gamma >= 0 && gamma <= rect.width()) + pointVectors.append(QVector2D(bx+gamma, by)); + // check left of rect: + bx = rect.left(); + by = rect.top(); + gamma = base.y()-by + (bx-base.x())*vec.y()/vec.x(); + if (gamma >= 0 && gamma <= rect.height()) + pointVectors.append(QVector2D(bx, by+gamma)); + // check right of rect: + bx = rect.right(); + by = rect.top(); + gamma = base.y()-by + (bx-base.x())*vec.y()/vec.x(); + if (gamma >= 0 && gamma <= rect.height()) + pointVectors.append(QVector2D(bx, by+gamma)); + + // evaluate points: + if (pointVectors.size() == 2) + { + result.setPoints(pointVectors.at(0).toPointF(), pointVectors.at(1).toPointF()); + } else if (pointVectors.size() > 2) + { + // line probably goes through corner of rect, and we got two points there. single out the point pair with greatest distance: + double distSqrMax = 0; + QVector2D pv1, pv2; + for (int i=0; i distSqrMax) + { + pv1 = pointVectors.at(i); + pv2 = pointVectors.at(k); + distSqrMax = distSqr; + } + } + } + result.setPoints(pv1.toPointF(), pv2.toPointF()); + } + } + return result; +} + +/*! \internal + + Returns the pen that should be used for drawing lines. Returns mPen when the + item is not selected and mSelectedPen when it is. +*/ +QPen QCPItemStraightLine::mainPen() const +{ + return mSelected ? mSelectedPen : mPen; +} + + +//////////////////////////////////////////////////////////////////////////////////////////////////// +//////////////////// QCPItemLine +//////////////////////////////////////////////////////////////////////////////////////////////////// + +/*! \class QCPItemLine + \brief A line from one point to another + + \image html QCPItemLine.png "Line example. Blue dotted circles are anchors, solid blue discs are positions." + + It has two positions, \a start and \a end, which define the end points of the line. + + With \ref setHead and \ref setTail you may set different line ending styles, e.g. to create an arrow. +*/ + +/*! + Creates a line item and sets default values. + + The constructed item can be added to the plot with QCustomPlot::addItem. +*/ +QCPItemLine::QCPItemLine(QCustomPlot *parentPlot) : + QCPAbstractItem(parentPlot), + start(createPosition(QLatin1String("start"))), + end(createPosition(QLatin1String("end"))) +{ + start->setCoords(0, 0); + end->setCoords(1, 1); + + setPen(QPen(Qt::black)); + setSelectedPen(QPen(Qt::blue,2)); +} + +QCPItemLine::~QCPItemLine() +{ +} + +/*! + Sets the pen that will be used to draw the line + + \see setSelectedPen +*/ +void QCPItemLine::setPen(const QPen &pen) +{ + mPen = pen; +} + +/*! + Sets the pen that will be used to draw the line when selected + + \see setPen, setSelected +*/ +void QCPItemLine::setSelectedPen(const QPen &pen) +{ + mSelectedPen = pen; +} + +/*! + Sets the line ending style of the head. The head corresponds to the \a end position. + + Note that due to the overloaded QCPLineEnding constructor, you may directly specify + a QCPLineEnding::EndingStyle here, e.g. \code setHead(QCPLineEnding::esSpikeArrow) \endcode + + \see setTail +*/ +void QCPItemLine::setHead(const QCPLineEnding &head) +{ + mHead = head; +} + +/*! + Sets the line ending style of the tail. The tail corresponds to the \a start position. + + Note that due to the overloaded QCPLineEnding constructor, you may directly specify + a QCPLineEnding::EndingStyle here, e.g. \code setTail(QCPLineEnding::esSpikeArrow) \endcode + + \see setHead +*/ +void QCPItemLine::setTail(const QCPLineEnding &tail) +{ + mTail = tail; +} + +/* inherits documentation from base class */ +double QCPItemLine::selectTest(const QPointF &pos, bool onlySelectable, QVariant *details) const +{ + Q_UNUSED(details) + if (onlySelectable && !mSelectable) + return -1; + + return qSqrt(distSqrToLine(start->pixelPoint(), end->pixelPoint(), pos)); +} + +/* inherits documentation from base class */ +void QCPItemLine::draw(QCPPainter *painter) +{ + QVector2D startVec(start->pixelPoint()); + QVector2D endVec(end->pixelPoint()); + if (startVec.toPoint() == endVec.toPoint()) + return; + // get visible segment of straight line inside clipRect: + double clipPad = qMax(mHead.boundingDistance(), mTail.boundingDistance()); + clipPad = qMax(clipPad, (double)mainPen().widthF()); + QLineF line = getRectClippedLine(startVec, endVec, clipRect().adjusted(-clipPad, -clipPad, clipPad, clipPad)); + // paint visible segment, if existent: + if (!line.isNull()) + { + painter->setPen(mainPen()); + painter->drawLine(line); + painter->setBrush(Qt::SolidPattern); + if (mTail.style() != QCPLineEnding::esNone) + mTail.draw(painter, startVec, startVec-endVec); + if (mHead.style() != QCPLineEnding::esNone) + mHead.draw(painter, endVec, endVec-startVec); + } +} + +/*! \internal + + Returns the section of the line defined by \a start and \a end, that is visible in the specified + \a rect. + + This is a helper function for \ref draw. +*/ +QLineF QCPItemLine::getRectClippedLine(const QVector2D &start, const QVector2D &end, const QRect &rect) const +{ + bool containsStart = rect.contains(start.x(), start.y()); + bool containsEnd = rect.contains(end.x(), end.y()); + if (containsStart && containsEnd) + return QLineF(start.toPointF(), end.toPointF()); + + QVector2D base = start; + QVector2D vec = end-start; + double bx, by; + double gamma, mu; + QLineF result; + QList pointVectors; + + if (!qFuzzyIsNull(vec.y())) // line is not horizontal + { + // check top of rect: + bx = rect.left(); + by = rect.top(); + mu = (by-base.y())/vec.y(); + if (mu >= 0 && mu <= 1) + { + gamma = base.x()-bx + mu*vec.x(); + if (gamma >= 0 && gamma <= rect.width()) + pointVectors.append(QVector2D(bx+gamma, by)); + } + // check bottom of rect: + bx = rect.left(); + by = rect.bottom(); + mu = (by-base.y())/vec.y(); + if (mu >= 0 && mu <= 1) + { + gamma = base.x()-bx + mu*vec.x(); + if (gamma >= 0 && gamma <= rect.width()) + pointVectors.append(QVector2D(bx+gamma, by)); + } + } + if (!qFuzzyIsNull(vec.x())) // line is not vertical + { + // check left of rect: + bx = rect.left(); + by = rect.top(); + mu = (bx-base.x())/vec.x(); + if (mu >= 0 && mu <= 1) + { + gamma = base.y()-by + mu*vec.y(); + if (gamma >= 0 && gamma <= rect.height()) + pointVectors.append(QVector2D(bx, by+gamma)); + } + // check right of rect: + bx = rect.right(); + by = rect.top(); + mu = (bx-base.x())/vec.x(); + if (mu >= 0 && mu <= 1) + { + gamma = base.y()-by + mu*vec.y(); + if (gamma >= 0 && gamma <= rect.height()) + pointVectors.append(QVector2D(bx, by+gamma)); + } + } + + if (containsStart) + pointVectors.append(start); + if (containsEnd) + pointVectors.append(end); + + // evaluate points: + if (pointVectors.size() == 2) + { + result.setPoints(pointVectors.at(0).toPointF(), pointVectors.at(1).toPointF()); + } else if (pointVectors.size() > 2) + { + // line probably goes through corner of rect, and we got two points there. single out the point pair with greatest distance: + double distSqrMax = 0; + QVector2D pv1, pv2; + for (int i=0; i distSqrMax) + { + pv1 = pointVectors.at(i); + pv2 = pointVectors.at(k); + distSqrMax = distSqr; + } + } + } + result.setPoints(pv1.toPointF(), pv2.toPointF()); + } + return result; +} + +/*! \internal + + Returns the pen that should be used for drawing lines. Returns mPen when the + item is not selected and mSelectedPen when it is. +*/ +QPen QCPItemLine::mainPen() const +{ + return mSelected ? mSelectedPen : mPen; +} + + +//////////////////////////////////////////////////////////////////////////////////////////////////// +//////////////////// QCPItemCurve +//////////////////////////////////////////////////////////////////////////////////////////////////// + +/*! \class QCPItemCurve + \brief A curved line from one point to another + + \image html QCPItemCurve.png "Curve example. Blue dotted circles are anchors, solid blue discs are positions." + + It has four positions, \a start and \a end, which define the end points of the line, and two + control points which define the direction the line exits from the start and the direction from + which it approaches the end: \a startDir and \a endDir. + + With \ref setHead and \ref setTail you may set different line ending styles, e.g. to create an + arrow. + + Often it is desirable for the control points to stay at fixed relative positions to the start/end + point. This can be achieved by setting the parent anchor e.g. of \a startDir simply to \a start, + and then specify the desired pixel offset with QCPItemPosition::setCoords on \a startDir. +*/ + +/*! + Creates a curve item and sets default values. + + The constructed item can be added to the plot with QCustomPlot::addItem. +*/ +QCPItemCurve::QCPItemCurve(QCustomPlot *parentPlot) : + QCPAbstractItem(parentPlot), + start(createPosition(QLatin1String("start"))), + startDir(createPosition(QLatin1String("startDir"))), + endDir(createPosition(QLatin1String("endDir"))), + end(createPosition(QLatin1String("end"))) +{ + start->setCoords(0, 0); + startDir->setCoords(0.5, 0); + endDir->setCoords(0, 0.5); + end->setCoords(1, 1); + + setPen(QPen(Qt::black)); + setSelectedPen(QPen(Qt::blue,2)); +} + +QCPItemCurve::~QCPItemCurve() +{ +} + +/*! + Sets the pen that will be used to draw the line + + \see setSelectedPen +*/ +void QCPItemCurve::setPen(const QPen &pen) +{ + mPen = pen; +} + +/*! + Sets the pen that will be used to draw the line when selected + + \see setPen, setSelected +*/ +void QCPItemCurve::setSelectedPen(const QPen &pen) +{ + mSelectedPen = pen; +} + +/*! + Sets the line ending style of the head. The head corresponds to the \a end position. + + Note that due to the overloaded QCPLineEnding constructor, you may directly specify + a QCPLineEnding::EndingStyle here, e.g. \code setHead(QCPLineEnding::esSpikeArrow) \endcode + + \see setTail +*/ +void QCPItemCurve::setHead(const QCPLineEnding &head) +{ + mHead = head; +} + +/*! + Sets the line ending style of the tail. The tail corresponds to the \a start position. + + Note that due to the overloaded QCPLineEnding constructor, you may directly specify + a QCPLineEnding::EndingStyle here, e.g. \code setTail(QCPLineEnding::esSpikeArrow) \endcode + + \see setHead +*/ +void QCPItemCurve::setTail(const QCPLineEnding &tail) +{ + mTail = tail; +} + +/* inherits documentation from base class */ +double QCPItemCurve::selectTest(const QPointF &pos, bool onlySelectable, QVariant *details) const +{ + Q_UNUSED(details) + if (onlySelectable && !mSelectable) + return -1; + + QPointF startVec(start->pixelPoint()); + QPointF startDirVec(startDir->pixelPoint()); + QPointF endDirVec(endDir->pixelPoint()); + QPointF endVec(end->pixelPoint()); + + QPainterPath cubicPath(startVec); + cubicPath.cubicTo(startDirVec, endDirVec, endVec); + + QPolygonF polygon = cubicPath.toSubpathPolygons().first(); + double minDistSqr = std::numeric_limits::max(); + for (int i=1; ipixelPoint()); + QPointF startDirVec(startDir->pixelPoint()); + QPointF endDirVec(endDir->pixelPoint()); + QPointF endVec(end->pixelPoint()); + if (QVector2D(endVec-startVec).length() > 1e10f) // too large curves cause crash + return; + + QPainterPath cubicPath(startVec); + cubicPath.cubicTo(startDirVec, endDirVec, endVec); + + // paint visible segment, if existent: + QRect clip = clipRect().adjusted(-mainPen().widthF(), -mainPen().widthF(), mainPen().widthF(), mainPen().widthF()); + QRect cubicRect = cubicPath.controlPointRect().toRect(); + if (cubicRect.isEmpty()) // may happen when start and end exactly on same x or y position + cubicRect.adjust(0, 0, 1, 1); + if (clip.intersects(cubicRect)) + { + painter->setPen(mainPen()); + painter->drawPath(cubicPath); + painter->setBrush(Qt::SolidPattern); + if (mTail.style() != QCPLineEnding::esNone) + mTail.draw(painter, QVector2D(startVec), M_PI-cubicPath.angleAtPercent(0)/180.0*M_PI); + if (mHead.style() != QCPLineEnding::esNone) + mHead.draw(painter, QVector2D(endVec), -cubicPath.angleAtPercent(1)/180.0*M_PI); + } +} + +/*! \internal + + Returns the pen that should be used for drawing lines. Returns mPen when the + item is not selected and mSelectedPen when it is. +*/ +QPen QCPItemCurve::mainPen() const +{ + return mSelected ? mSelectedPen : mPen; +} + + +//////////////////////////////////////////////////////////////////////////////////////////////////// +//////////////////// QCPItemRect +//////////////////////////////////////////////////////////////////////////////////////////////////// + +/*! \class QCPItemRect + \brief A rectangle + + \image html QCPItemRect.png "Rectangle example. Blue dotted circles are anchors, solid blue discs are positions." + + It has two positions, \a topLeft and \a bottomRight, which define the rectangle. +*/ + +/*! + Creates a rectangle item and sets default values. + + The constructed item can be added to the plot with QCustomPlot::addItem. +*/ +QCPItemRect::QCPItemRect(QCustomPlot *parentPlot) : + QCPAbstractItem(parentPlot), + topLeft(createPosition(QLatin1String("topLeft"))), + bottomRight(createPosition(QLatin1String("bottomRight"))), + top(createAnchor(QLatin1String("top"), aiTop)), + topRight(createAnchor(QLatin1String("topRight"), aiTopRight)), + right(createAnchor(QLatin1String("right"), aiRight)), + bottom(createAnchor(QLatin1String("bottom"), aiBottom)), + bottomLeft(createAnchor(QLatin1String("bottomLeft"), aiBottomLeft)), + left(createAnchor(QLatin1String("left"), aiLeft)) +{ + topLeft->setCoords(0, 1); + bottomRight->setCoords(1, 0); + + setPen(QPen(Qt::black)); + setSelectedPen(QPen(Qt::blue,2)); + setBrush(Qt::NoBrush); + setSelectedBrush(Qt::NoBrush); +} + +QCPItemRect::~QCPItemRect() +{ +} + +/*! + Sets the pen that will be used to draw the line of the rectangle + + \see setSelectedPen, setBrush +*/ +void QCPItemRect::setPen(const QPen &pen) +{ + mPen = pen; +} + +/*! + Sets the pen that will be used to draw the line of the rectangle when selected + + \see setPen, setSelected +*/ +void QCPItemRect::setSelectedPen(const QPen &pen) +{ + mSelectedPen = pen; +} + +/*! + Sets the brush that will be used to fill the rectangle. To disable filling, set \a brush to + Qt::NoBrush. + + \see setSelectedBrush, setPen +*/ +void QCPItemRect::setBrush(const QBrush &brush) +{ + mBrush = brush; +} + +/*! + Sets the brush that will be used to fill the rectangle when selected. To disable filling, set \a + brush to Qt::NoBrush. + + \see setBrush +*/ +void QCPItemRect::setSelectedBrush(const QBrush &brush) +{ + mSelectedBrush = brush; +} + +/* inherits documentation from base class */ +double QCPItemRect::selectTest(const QPointF &pos, bool onlySelectable, QVariant *details) const +{ + Q_UNUSED(details) + if (onlySelectable && !mSelectable) + return -1; + + QRectF rect = QRectF(topLeft->pixelPoint(), bottomRight->pixelPoint()).normalized(); + bool filledRect = mBrush.style() != Qt::NoBrush && mBrush.color().alpha() != 0; + return rectSelectTest(rect, pos, filledRect); +} + +/* inherits documentation from base class */ +void QCPItemRect::draw(QCPPainter *painter) +{ + QPointF p1 = topLeft->pixelPoint(); + QPointF p2 = bottomRight->pixelPoint(); + if (p1.toPoint() == p2.toPoint()) + return; + QRectF rect = QRectF(p1, p2).normalized(); + double clipPad = mainPen().widthF(); + QRectF boundingRect = rect.adjusted(-clipPad, -clipPad, clipPad, clipPad); + if (boundingRect.intersects(clipRect())) // only draw if bounding rect of rect item is visible in cliprect + { + painter->setPen(mainPen()); + painter->setBrush(mainBrush()); + painter->drawRect(rect); + } +} + +/* inherits documentation from base class */ +QPointF QCPItemRect::anchorPixelPoint(int anchorId) const +{ + QRectF rect = QRectF(topLeft->pixelPoint(), bottomRight->pixelPoint()); + switch (anchorId) + { + case aiTop: return (rect.topLeft()+rect.topRight())*0.5; + case aiTopRight: return rect.topRight(); + case aiRight: return (rect.topRight()+rect.bottomRight())*0.5; + case aiBottom: return (rect.bottomLeft()+rect.bottomRight())*0.5; + case aiBottomLeft: return rect.bottomLeft(); + case aiLeft: return (rect.topLeft()+rect.bottomLeft())*0.5; + } + + qDebug() << Q_FUNC_INFO << "invalid anchorId" << anchorId; + return QPointF(); +} + +/*! \internal + + Returns the pen that should be used for drawing lines. Returns mPen when the item is not selected + and mSelectedPen when it is. +*/ +QPen QCPItemRect::mainPen() const +{ + return mSelected ? mSelectedPen : mPen; +} + +/*! \internal + + Returns the brush that should be used for drawing fills of the item. Returns mBrush when the item + is not selected and mSelectedBrush when it is. +*/ +QBrush QCPItemRect::mainBrush() const +{ + return mSelected ? mSelectedBrush : mBrush; +} + + +//////////////////////////////////////////////////////////////////////////////////////////////////// +//////////////////// QCPItemText +//////////////////////////////////////////////////////////////////////////////////////////////////// + +/*! \class QCPItemText + \brief A text label + + \image html QCPItemText.png "Text example. Blue dotted circles are anchors, solid blue discs are positions." + + Its position is defined by the member \a position and the setting of \ref setPositionAlignment. + The latter controls which part of the text rect shall be aligned with \a position. + + The text alignment itself (i.e. left, center, right) can be controlled with \ref + setTextAlignment. + + The text may be rotated around the \a position point with \ref setRotation. +*/ + +/*! + Creates a text item and sets default values. + + The constructed item can be added to the plot with QCustomPlot::addItem. +*/ +QCPItemText::QCPItemText(QCustomPlot *parentPlot) : + QCPAbstractItem(parentPlot), + position(createPosition(QLatin1String("position"))), + topLeft(createAnchor(QLatin1String("topLeft"), aiTopLeft)), + top(createAnchor(QLatin1String("top"), aiTop)), + topRight(createAnchor(QLatin1String("topRight"), aiTopRight)), + right(createAnchor(QLatin1String("right"), aiRight)), + bottomRight(createAnchor(QLatin1String("bottomRight"), aiBottomRight)), + bottom(createAnchor(QLatin1String("bottom"), aiBottom)), + bottomLeft(createAnchor(QLatin1String("bottomLeft"), aiBottomLeft)), + left(createAnchor(QLatin1String("left"), aiLeft)) +{ + position->setCoords(0, 0); + + setRotation(0); + setTextAlignment(Qt::AlignTop|Qt::AlignHCenter); + setPositionAlignment(Qt::AlignCenter); + setText(QLatin1String("text")); + + setPen(Qt::NoPen); + setSelectedPen(Qt::NoPen); + setBrush(Qt::NoBrush); + setSelectedBrush(Qt::NoBrush); + setColor(Qt::black); + setSelectedColor(Qt::blue); +} + +QCPItemText::~QCPItemText() +{ +} + +/*! + Sets the color of the text. +*/ +void QCPItemText::setColor(const QColor &color) +{ + mColor = color; +} + +/*! + Sets the color of the text that will be used when the item is selected. +*/ +void QCPItemText::setSelectedColor(const QColor &color) +{ + mSelectedColor = color; +} + +/*! + Sets the pen that will be used do draw a rectangular border around the text. To disable the + border, set \a pen to Qt::NoPen. + + \see setSelectedPen, setBrush, setPadding +*/ +void QCPItemText::setPen(const QPen &pen) +{ + mPen = pen; +} + +/*! + Sets the pen that will be used do draw a rectangular border around the text, when the item is + selected. To disable the border, set \a pen to Qt::NoPen. + + \see setPen +*/ +void QCPItemText::setSelectedPen(const QPen &pen) +{ + mSelectedPen = pen; +} + +/*! + Sets the brush that will be used do fill the background of the text. To disable the + background, set \a brush to Qt::NoBrush. + + \see setSelectedBrush, setPen, setPadding +*/ +void QCPItemText::setBrush(const QBrush &brush) +{ + mBrush = brush; +} + +/*! + Sets the brush that will be used do fill the background of the text, when the item is selected. To disable the + background, set \a brush to Qt::NoBrush. + + \see setBrush +*/ +void QCPItemText::setSelectedBrush(const QBrush &brush) +{ + mSelectedBrush = brush; +} + +/*! + Sets the font of the text. + + \see setSelectedFont, setColor +*/ +void QCPItemText::setFont(const QFont &font) +{ + mFont = font; +} + +/*! + Sets the font of the text that will be used when the item is selected. + + \see setFont +*/ +void QCPItemText::setSelectedFont(const QFont &font) +{ + mSelectedFont = font; +} + +/*! + Sets the text that will be displayed. Multi-line texts are supported by inserting a line break + character, e.g. '\n'. + + \see setFont, setColor, setTextAlignment +*/ +void QCPItemText::setText(const QString &text) +{ + mText = text; +} + +/*! + Sets which point of the text rect shall be aligned with \a position. + + Examples: + \li If \a alignment is Qt::AlignHCenter | Qt::AlignTop, the text will be positioned such + that the top of the text rect will be horizontally centered on \a position. + \li If \a alignment is Qt::AlignLeft | Qt::AlignBottom, \a position will indicate the + bottom left corner of the text rect. + + If you want to control the alignment of (multi-lined) text within the text rect, use \ref + setTextAlignment. +*/ +void QCPItemText::setPositionAlignment(Qt::Alignment alignment) +{ + mPositionAlignment = alignment; +} + +/*! + Controls how (multi-lined) text is aligned inside the text rect (typically Qt::AlignLeft, Qt::AlignCenter or Qt::AlignRight). +*/ +void QCPItemText::setTextAlignment(Qt::Alignment alignment) +{ + mTextAlignment = alignment; +} + +/*! + Sets the angle in degrees by which the text (and the text rectangle, if visible) will be rotated + around \a position. +*/ +void QCPItemText::setRotation(double degrees) +{ + mRotation = degrees; +} + +/*! + Sets the distance between the border of the text rectangle and the text. The appearance (and + visibility) of the text rectangle can be controlled with \ref setPen and \ref setBrush. +*/ +void QCPItemText::setPadding(const QMargins &padding) +{ + mPadding = padding; +} + +/* inherits documentation from base class */ +double QCPItemText::selectTest(const QPointF &pos, bool onlySelectable, QVariant *details) const +{ + Q_UNUSED(details) + if (onlySelectable && !mSelectable) + return -1; + + // The rect may be rotated, so we transform the actual clicked pos to the rotated + // coordinate system, so we can use the normal rectSelectTest function for non-rotated rects: + QPointF positionPixels(position->pixelPoint()); + QTransform inputTransform; + inputTransform.translate(positionPixels.x(), positionPixels.y()); + inputTransform.rotate(-mRotation); + inputTransform.translate(-positionPixels.x(), -positionPixels.y()); + QPointF rotatedPos = inputTransform.map(pos); + QFontMetrics fontMetrics(mFont); + QRect textRect = fontMetrics.boundingRect(0, 0, 0, 0, Qt::TextDontClip|mTextAlignment, mText); + QRect textBoxRect = textRect.adjusted(-mPadding.left(), -mPadding.top(), mPadding.right(), mPadding.bottom()); + QPointF textPos = getTextDrawPoint(positionPixels, textBoxRect, mPositionAlignment); + textBoxRect.moveTopLeft(textPos.toPoint()); + + return rectSelectTest(textBoxRect, rotatedPos, true); +} + +/* inherits documentation from base class */ +void QCPItemText::draw(QCPPainter *painter) +{ + QPointF pos(position->pixelPoint()); + QTransform transform = painter->transform(); + transform.translate(pos.x(), pos.y()); + if (!qFuzzyIsNull(mRotation)) + transform.rotate(mRotation); + painter->setFont(mainFont()); + QRect textRect = painter->fontMetrics().boundingRect(0, 0, 0, 0, Qt::TextDontClip|mTextAlignment, mText); + QRect textBoxRect = textRect.adjusted(-mPadding.left(), -mPadding.top(), mPadding.right(), mPadding.bottom()); + QPointF textPos = getTextDrawPoint(QPointF(0, 0), textBoxRect, mPositionAlignment); // 0, 0 because the transform does the translation + textRect.moveTopLeft(textPos.toPoint()+QPoint(mPadding.left(), mPadding.top())); + textBoxRect.moveTopLeft(textPos.toPoint()); + double clipPad = mainPen().widthF(); + QRect boundingRect = textBoxRect.adjusted(-clipPad, -clipPad, clipPad, clipPad); + if (transform.mapRect(boundingRect).intersects(painter->transform().mapRect(clipRect()))) + { + painter->setTransform(transform); + if ((mainBrush().style() != Qt::NoBrush && mainBrush().color().alpha() != 0) || + (mainPen().style() != Qt::NoPen && mainPen().color().alpha() != 0)) + { + painter->setPen(mainPen()); + painter->setBrush(mainBrush()); + painter->drawRect(textBoxRect); + } + painter->setBrush(Qt::NoBrush); + painter->setPen(QPen(mainColor())); + painter->drawText(textRect, Qt::TextDontClip|mTextAlignment, mText); + } +} + +/* inherits documentation from base class */ +QPointF QCPItemText::anchorPixelPoint(int anchorId) const +{ + // get actual rect points (pretty much copied from draw function): + QPointF pos(position->pixelPoint()); + QTransform transform; + transform.translate(pos.x(), pos.y()); + if (!qFuzzyIsNull(mRotation)) + transform.rotate(mRotation); + QFontMetrics fontMetrics(mainFont()); + QRect textRect = fontMetrics.boundingRect(0, 0, 0, 0, Qt::TextDontClip|mTextAlignment, mText); + QRectF textBoxRect = textRect.adjusted(-mPadding.left(), -mPadding.top(), mPadding.right(), mPadding.bottom()); + QPointF textPos = getTextDrawPoint(QPointF(0, 0), textBoxRect, mPositionAlignment); // 0, 0 because the transform does the translation + textBoxRect.moveTopLeft(textPos.toPoint()); + QPolygonF rectPoly = transform.map(QPolygonF(textBoxRect)); + + switch (anchorId) + { + case aiTopLeft: return rectPoly.at(0); + case aiTop: return (rectPoly.at(0)+rectPoly.at(1))*0.5; + case aiTopRight: return rectPoly.at(1); + case aiRight: return (rectPoly.at(1)+rectPoly.at(2))*0.5; + case aiBottomRight: return rectPoly.at(2); + case aiBottom: return (rectPoly.at(2)+rectPoly.at(3))*0.5; + case aiBottomLeft: return rectPoly.at(3); + case aiLeft: return (rectPoly.at(3)+rectPoly.at(0))*0.5; + } + + qDebug() << Q_FUNC_INFO << "invalid anchorId" << anchorId; + return QPointF(); +} + +/*! \internal + + Returns the point that must be given to the QPainter::drawText function (which expects the top + left point of the text rect), according to the position \a pos, the text bounding box \a rect and + the requested \a positionAlignment. + + For example, if \a positionAlignment is Qt::AlignLeft | Qt::AlignBottom the returned point + will be shifted upward by the height of \a rect, starting from \a pos. So if the text is finally + drawn at that point, the lower left corner of the resulting text rect is at \a pos. +*/ +QPointF QCPItemText::getTextDrawPoint(const QPointF &pos, const QRectF &rect, Qt::Alignment positionAlignment) const +{ + if (positionAlignment == 0 || positionAlignment == (Qt::AlignLeft|Qt::AlignTop)) + return pos; + + QPointF result = pos; // start at top left + if (positionAlignment.testFlag(Qt::AlignHCenter)) + result.rx() -= rect.width()/2.0; + else if (positionAlignment.testFlag(Qt::AlignRight)) + result.rx() -= rect.width(); + if (positionAlignment.testFlag(Qt::AlignVCenter)) + result.ry() -= rect.height()/2.0; + else if (positionAlignment.testFlag(Qt::AlignBottom)) + result.ry() -= rect.height(); + return result; +} + +/*! \internal + + Returns the font that should be used for drawing text. Returns mFont when the item is not selected + and mSelectedFont when it is. +*/ +QFont QCPItemText::mainFont() const +{ + return mSelected ? mSelectedFont : mFont; +} + +/*! \internal + + Returns the color that should be used for drawing text. Returns mColor when the item is not + selected and mSelectedColor when it is. +*/ +QColor QCPItemText::mainColor() const +{ + return mSelected ? mSelectedColor : mColor; +} + +/*! \internal + + Returns the pen that should be used for drawing lines. Returns mPen when the item is not selected + and mSelectedPen when it is. +*/ +QPen QCPItemText::mainPen() const +{ + return mSelected ? mSelectedPen : mPen; +} + +/*! \internal + + Returns the brush that should be used for drawing fills of the item. Returns mBrush when the item + is not selected and mSelectedBrush when it is. +*/ +QBrush QCPItemText::mainBrush() const +{ + return mSelected ? mSelectedBrush : mBrush; +} + + +//////////////////////////////////////////////////////////////////////////////////////////////////// +//////////////////// QCPItemEllipse +//////////////////////////////////////////////////////////////////////////////////////////////////// + +/*! \class QCPItemEllipse + \brief An ellipse + + \image html QCPItemEllipse.png "Ellipse example. Blue dotted circles are anchors, solid blue discs are positions." + + It has two positions, \a topLeft and \a bottomRight, which define the rect the ellipse will be drawn in. +*/ + +/*! + Creates an ellipse item and sets default values. + + The constructed item can be added to the plot with QCustomPlot::addItem. +*/ +QCPItemEllipse::QCPItemEllipse(QCustomPlot *parentPlot) : + QCPAbstractItem(parentPlot), + topLeft(createPosition(QLatin1String("topLeft"))), + bottomRight(createPosition(QLatin1String("bottomRight"))), + topLeftRim(createAnchor(QLatin1String("topLeftRim"), aiTopLeftRim)), + top(createAnchor(QLatin1String("top"), aiTop)), + topRightRim(createAnchor(QLatin1String("topRightRim"), aiTopRightRim)), + right(createAnchor(QLatin1String("right"), aiRight)), + bottomRightRim(createAnchor(QLatin1String("bottomRightRim"), aiBottomRightRim)), + bottom(createAnchor(QLatin1String("bottom"), aiBottom)), + bottomLeftRim(createAnchor(QLatin1String("bottomLeftRim"), aiBottomLeftRim)), + left(createAnchor(QLatin1String("left"), aiLeft)), + center(createAnchor(QLatin1String("center"), aiCenter)) +{ + topLeft->setCoords(0, 1); + bottomRight->setCoords(1, 0); + + setPen(QPen(Qt::black)); + setSelectedPen(QPen(Qt::blue, 2)); + setBrush(Qt::NoBrush); + setSelectedBrush(Qt::NoBrush); +} + +QCPItemEllipse::~QCPItemEllipse() +{ +} + +/*! + Sets the pen that will be used to draw the line of the ellipse + + \see setSelectedPen, setBrush +*/ +void QCPItemEllipse::setPen(const QPen &pen) +{ + mPen = pen; +} + +/*! + Sets the pen that will be used to draw the line of the ellipse when selected + + \see setPen, setSelected +*/ +void QCPItemEllipse::setSelectedPen(const QPen &pen) +{ + mSelectedPen = pen; +} + +/*! + Sets the brush that will be used to fill the ellipse. To disable filling, set \a brush to + Qt::NoBrush. + + \see setSelectedBrush, setPen +*/ +void QCPItemEllipse::setBrush(const QBrush &brush) +{ + mBrush = brush; +} + +/*! + Sets the brush that will be used to fill the ellipse when selected. To disable filling, set \a + brush to Qt::NoBrush. + + \see setBrush +*/ +void QCPItemEllipse::setSelectedBrush(const QBrush &brush) +{ + mSelectedBrush = brush; +} + +/* inherits documentation from base class */ +double QCPItemEllipse::selectTest(const QPointF &pos, bool onlySelectable, QVariant *details) const +{ + Q_UNUSED(details) + if (onlySelectable && !mSelectable) + return -1; + + double result = -1; + QPointF p1 = topLeft->pixelPoint(); + QPointF p2 = bottomRight->pixelPoint(); + QPointF center((p1+p2)/2.0); + double a = qAbs(p1.x()-p2.x())/2.0; + double b = qAbs(p1.y()-p2.y())/2.0; + double x = pos.x()-center.x(); + double y = pos.y()-center.y(); + + // distance to border: + double c = 1.0/qSqrt(x*x/(a*a)+y*y/(b*b)); + result = qAbs(c-1)*qSqrt(x*x+y*y); + // filled ellipse, allow click inside to count as hit: + if (result > mParentPlot->selectionTolerance()*0.99 && mBrush.style() != Qt::NoBrush && mBrush.color().alpha() != 0) + { + if (x*x/(a*a) + y*y/(b*b) <= 1) + result = mParentPlot->selectionTolerance()*0.99; + } + return result; +} + +/* inherits documentation from base class */ +void QCPItemEllipse::draw(QCPPainter *painter) +{ + QPointF p1 = topLeft->pixelPoint(); + QPointF p2 = bottomRight->pixelPoint(); + if (p1.toPoint() == p2.toPoint()) + return; + QRectF ellipseRect = QRectF(p1, p2).normalized(); + QRect clip = clipRect().adjusted(-mainPen().widthF(), -mainPen().widthF(), mainPen().widthF(), mainPen().widthF()); + if (ellipseRect.intersects(clip)) // only draw if bounding rect of ellipse is visible in cliprect + { + painter->setPen(mainPen()); + painter->setBrush(mainBrush()); +#ifdef __EXCEPTIONS + try // drawEllipse sometimes throws exceptions if ellipse is too big + { +#endif + painter->drawEllipse(ellipseRect); +#ifdef __EXCEPTIONS + } catch (...) + { + qDebug() << Q_FUNC_INFO << "Item too large for memory, setting invisible"; + setVisible(false); + } +#endif + } +} + +/* inherits documentation from base class */ +QPointF QCPItemEllipse::anchorPixelPoint(int anchorId) const +{ + QRectF rect = QRectF(topLeft->pixelPoint(), bottomRight->pixelPoint()); + switch (anchorId) + { + case aiTopLeftRim: return rect.center()+(rect.topLeft()-rect.center())*1/qSqrt(2); + case aiTop: return (rect.topLeft()+rect.topRight())*0.5; + case aiTopRightRim: return rect.center()+(rect.topRight()-rect.center())*1/qSqrt(2); + case aiRight: return (rect.topRight()+rect.bottomRight())*0.5; + case aiBottomRightRim: return rect.center()+(rect.bottomRight()-rect.center())*1/qSqrt(2); + case aiBottom: return (rect.bottomLeft()+rect.bottomRight())*0.5; + case aiBottomLeftRim: return rect.center()+(rect.bottomLeft()-rect.center())*1/qSqrt(2); + case aiLeft: return (rect.topLeft()+rect.bottomLeft())*0.5; + case aiCenter: return (rect.topLeft()+rect.bottomRight())*0.5; + } + + qDebug() << Q_FUNC_INFO << "invalid anchorId" << anchorId; + return QPointF(); +} + +/*! \internal + + Returns the pen that should be used for drawing lines. Returns mPen when the item is not selected + and mSelectedPen when it is. +*/ +QPen QCPItemEllipse::mainPen() const +{ + return mSelected ? mSelectedPen : mPen; +} + +/*! \internal + + Returns the brush that should be used for drawing fills of the item. Returns mBrush when the item + is not selected and mSelectedBrush when it is. +*/ +QBrush QCPItemEllipse::mainBrush() const +{ + return mSelected ? mSelectedBrush : mBrush; +} + + +//////////////////////////////////////////////////////////////////////////////////////////////////// +//////////////////// QCPItemPixmap +//////////////////////////////////////////////////////////////////////////////////////////////////// + +/*! \class QCPItemPixmap + \brief An arbitrary pixmap + + \image html QCPItemPixmap.png "Pixmap example. Blue dotted circles are anchors, solid blue discs are positions." + + It has two positions, \a topLeft and \a bottomRight, which define the rectangle the pixmap will + be drawn in. Depending on the scale setting (\ref setScaled), the pixmap will be either scaled to + fit the rectangle or be drawn aligned to the topLeft position. + + If scaling is enabled and \a topLeft is further to the bottom/right than \a bottomRight (as shown + on the right side of the example image), the pixmap will be flipped in the respective + orientations. +*/ + +/*! + Creates a rectangle item and sets default values. + + The constructed item can be added to the plot with QCustomPlot::addItem. +*/ +QCPItemPixmap::QCPItemPixmap(QCustomPlot *parentPlot) : + QCPAbstractItem(parentPlot), + topLeft(createPosition(QLatin1String("topLeft"))), + bottomRight(createPosition(QLatin1String("bottomRight"))), + top(createAnchor(QLatin1String("top"), aiTop)), + topRight(createAnchor(QLatin1String("topRight"), aiTopRight)), + right(createAnchor(QLatin1String("right"), aiRight)), + bottom(createAnchor(QLatin1String("bottom"), aiBottom)), + bottomLeft(createAnchor(QLatin1String("bottomLeft"), aiBottomLeft)), + left(createAnchor(QLatin1String("left"), aiLeft)), + mScaledPixmapInvalidated(true) +{ + topLeft->setCoords(0, 1); + bottomRight->setCoords(1, 0); + + setPen(Qt::NoPen); + setSelectedPen(QPen(Qt::blue)); + setScaled(false, Qt::KeepAspectRatio, Qt::SmoothTransformation); +} + +QCPItemPixmap::~QCPItemPixmap() +{ +} + +/*! + Sets the pixmap that will be displayed. +*/ +void QCPItemPixmap::setPixmap(const QPixmap &pixmap) +{ + mPixmap = pixmap; + mScaledPixmapInvalidated = true; + if (mPixmap.isNull()) + qDebug() << Q_FUNC_INFO << "pixmap is null"; +} + +/*! + Sets whether the pixmap will be scaled to fit the rectangle defined by the \a topLeft and \a + bottomRight positions. +*/ +void QCPItemPixmap::setScaled(bool scaled, Qt::AspectRatioMode aspectRatioMode, Qt::TransformationMode transformationMode) +{ + mScaled = scaled; + mAspectRatioMode = aspectRatioMode; + mTransformationMode = transformationMode; + mScaledPixmapInvalidated = true; +} + +/*! + Sets the pen that will be used to draw a border around the pixmap. + + \see setSelectedPen, setBrush +*/ +void QCPItemPixmap::setPen(const QPen &pen) +{ + mPen = pen; +} + +/*! + Sets the pen that will be used to draw a border around the pixmap when selected + + \see setPen, setSelected +*/ +void QCPItemPixmap::setSelectedPen(const QPen &pen) +{ + mSelectedPen = pen; +} + +/* inherits documentation from base class */ +double QCPItemPixmap::selectTest(const QPointF &pos, bool onlySelectable, QVariant *details) const +{ + Q_UNUSED(details) + if (onlySelectable && !mSelectable) + return -1; + + return rectSelectTest(getFinalRect(), pos, true); +} + +/* inherits documentation from base class */ +void QCPItemPixmap::draw(QCPPainter *painter) +{ + bool flipHorz = false; + bool flipVert = false; + QRect rect = getFinalRect(&flipHorz, &flipVert); + double clipPad = mainPen().style() == Qt::NoPen ? 0 : mainPen().widthF(); + QRect boundingRect = rect.adjusted(-clipPad, -clipPad, clipPad, clipPad); + if (boundingRect.intersects(clipRect())) + { + updateScaledPixmap(rect, flipHorz, flipVert); + painter->drawPixmap(rect.topLeft(), mScaled ? mScaledPixmap : mPixmap); + QPen pen = mainPen(); + if (pen.style() != Qt::NoPen) + { + painter->setPen(pen); + painter->setBrush(Qt::NoBrush); + painter->drawRect(rect); + } + } +} + +/* inherits documentation from base class */ +QPointF QCPItemPixmap::anchorPixelPoint(int anchorId) const +{ + bool flipHorz; + bool flipVert; + QRect rect = getFinalRect(&flipHorz, &flipVert); + // we actually want denormal rects (negative width/height) here, so restore + // the flipped state: + if (flipHorz) + rect.adjust(rect.width(), 0, -rect.width(), 0); + if (flipVert) + rect.adjust(0, rect.height(), 0, -rect.height()); + + switch (anchorId) + { + case aiTop: return (rect.topLeft()+rect.topRight())*0.5; + case aiTopRight: return rect.topRight(); + case aiRight: return (rect.topRight()+rect.bottomRight())*0.5; + case aiBottom: return (rect.bottomLeft()+rect.bottomRight())*0.5; + case aiBottomLeft: return rect.bottomLeft(); + case aiLeft: return (rect.topLeft()+rect.bottomLeft())*0.5;; + } + + qDebug() << Q_FUNC_INFO << "invalid anchorId" << anchorId; + return QPointF(); +} + +/*! \internal + + Creates the buffered scaled image (\a mScaledPixmap) to fit the specified \a finalRect. The + parameters \a flipHorz and \a flipVert control whether the resulting image shall be flipped + horizontally or vertically. (This is used when \a topLeft is further to the bottom/right than \a + bottomRight.) + + This function only creates the scaled pixmap when the buffered pixmap has a different size than + the expected result, so calling this function repeatedly, e.g. in the \ref draw function, does + not cause expensive rescaling every time. + + If scaling is disabled, sets mScaledPixmap to a null QPixmap. +*/ +void QCPItemPixmap::updateScaledPixmap(QRect finalRect, bool flipHorz, bool flipVert) +{ + if (mPixmap.isNull()) + return; + + if (mScaled) + { + if (finalRect.isNull()) + finalRect = getFinalRect(&flipHorz, &flipVert); + if (mScaledPixmapInvalidated || finalRect.size() != mScaledPixmap.size()) + { + mScaledPixmap = mPixmap.scaled(finalRect.size(), mAspectRatioMode, mTransformationMode); + if (flipHorz || flipVert) + mScaledPixmap = QPixmap::fromImage(mScaledPixmap.toImage().mirrored(flipHorz, flipVert)); + } + } else if (!mScaledPixmap.isNull()) + mScaledPixmap = QPixmap(); + mScaledPixmapInvalidated = false; +} + +/*! \internal + + Returns the final (tight) rect the pixmap is drawn in, depending on the current item positions + and scaling settings. + + The output parameters \a flippedHorz and \a flippedVert return whether the pixmap should be drawn + flipped horizontally or vertically in the returned rect. (The returned rect itself is always + normalized, i.e. the top left corner of the rect is actually further to the top/left than the + bottom right corner). This is the case when the item position \a topLeft is further to the + bottom/right than \a bottomRight. + + If scaling is disabled, returns a rect with size of the original pixmap and the top left corner + aligned with the item position \a topLeft. The position \a bottomRight is ignored. +*/ +QRect QCPItemPixmap::getFinalRect(bool *flippedHorz, bool *flippedVert) const +{ + QRect result; + bool flipHorz = false; + bool flipVert = false; + QPoint p1 = topLeft->pixelPoint().toPoint(); + QPoint p2 = bottomRight->pixelPoint().toPoint(); + if (p1 == p2) + return QRect(p1, QSize(0, 0)); + if (mScaled) + { + QSize newSize = QSize(p2.x()-p1.x(), p2.y()-p1.y()); + QPoint topLeft = p1; + if (newSize.width() < 0) + { + flipHorz = true; + newSize.rwidth() *= -1; + topLeft.setX(p2.x()); + } + if (newSize.height() < 0) + { + flipVert = true; + newSize.rheight() *= -1; + topLeft.setY(p2.y()); + } + QSize scaledSize = mPixmap.size(); + scaledSize.scale(newSize, mAspectRatioMode); + result = QRect(topLeft, scaledSize); + } else + { + result = QRect(p1, mPixmap.size()); + } + if (flippedHorz) + *flippedHorz = flipHorz; + if (flippedVert) + *flippedVert = flipVert; + return result; +} + +/*! \internal + + Returns the pen that should be used for drawing lines. Returns mPen when the item is not selected + and mSelectedPen when it is. +*/ +QPen QCPItemPixmap::mainPen() const +{ + return mSelected ? mSelectedPen : mPen; +} + + +//////////////////////////////////////////////////////////////////////////////////////////////////// +//////////////////// QCPItemTracer +//////////////////////////////////////////////////////////////////////////////////////////////////// + +/*! \class QCPItemTracer + \brief Item that sticks to QCPGraph data points + + \image html QCPItemTracer.png "Tracer example. Blue dotted circles are anchors, solid blue discs are positions." + + The tracer can be connected with a QCPGraph via \ref setGraph. Then it will automatically adopt + the coordinate axes of the graph and update its \a position to be on the graph's data. This means + the key stays controllable via \ref setGraphKey, but the value will follow the graph data. If a + QCPGraph is connected, note that setting the coordinates of the tracer item directly via \a + position will have no effect because they will be overriden in the next redraw (this is when the + coordinate update happens). + + If the specified key in \ref setGraphKey is outside the key bounds of the graph, the tracer will + stay at the corresponding end of the graph. + + With \ref setInterpolating you may specify whether the tracer may only stay exactly on data + points or whether it interpolates data points linearly, if given a key that lies between two data + points of the graph. + + The tracer has different visual styles, see \ref setStyle. It is also possible to make the tracer + have no own visual appearance (set the style to \ref tsNone), and just connect other item + positions to the tracer \a position (used as an anchor) via \ref + QCPItemPosition::setParentAnchor. + + \note The tracer position is only automatically updated upon redraws. So when the data of the + graph changes and immediately afterwards (without a redraw) the a position coordinates of the + tracer are retrieved, they will not reflect the updated data of the graph. In this case \ref + updatePosition must be called manually, prior to reading the tracer coordinates. +*/ + +/*! + Creates a tracer item and sets default values. + + The constructed item can be added to the plot with QCustomPlot::addItem. +*/ +QCPItemTracer::QCPItemTracer(QCustomPlot *parentPlot) : + QCPAbstractItem(parentPlot), + position(createPosition(QLatin1String("position"))), + mGraph(0) +{ + position->setCoords(0, 0); + + setBrush(Qt::NoBrush); + setSelectedBrush(Qt::NoBrush); + setPen(QPen(Qt::black)); + setSelectedPen(QPen(Qt::blue, 2)); + setStyle(tsCrosshair); + setSize(6); + setInterpolating(false); + setGraphKey(0); +} + +QCPItemTracer::~QCPItemTracer() +{ +} + +/*! + Sets the pen that will be used to draw the line of the tracer + + \see setSelectedPen, setBrush +*/ +void QCPItemTracer::setPen(const QPen &pen) +{ + mPen = pen; +} + +/*! + Sets the pen that will be used to draw the line of the tracer when selected + + \see setPen, setSelected +*/ +void QCPItemTracer::setSelectedPen(const QPen &pen) +{ + mSelectedPen = pen; +} + +/*! + Sets the brush that will be used to draw any fills of the tracer + + \see setSelectedBrush, setPen +*/ +void QCPItemTracer::setBrush(const QBrush &brush) +{ + mBrush = brush; +} + +/*! + Sets the brush that will be used to draw any fills of the tracer, when selected. + + \see setBrush, setSelected +*/ +void QCPItemTracer::setSelectedBrush(const QBrush &brush) +{ + mSelectedBrush = brush; +} + +/*! + Sets the size of the tracer in pixels, if the style supports setting a size (e.g. \ref tsSquare + does, \ref tsCrosshair does not). +*/ +void QCPItemTracer::setSize(double size) +{ + mSize = size; +} + +/*! + Sets the style/visual appearance of the tracer. + + If you only want to use the tracer \a position as an anchor for other items, set \a style to + \ref tsNone. +*/ +void QCPItemTracer::setStyle(QCPItemTracer::TracerStyle style) +{ + mStyle = style; +} + +/*! + Sets the QCPGraph this tracer sticks to. The tracer \a position will be set to type + QCPItemPosition::ptPlotCoords and the axes will be set to the axes of \a graph. + + To free the tracer from any graph, set \a graph to 0. The tracer \a position can then be placed + freely like any other item position. This is the state the tracer will assume when its graph gets + deleted while still attached to it. + + \see setGraphKey +*/ +void QCPItemTracer::setGraph(QCPGraph *graph) +{ + if (graph) + { + if (graph->parentPlot() == mParentPlot) + { + position->setType(QCPItemPosition::ptPlotCoords); + position->setAxes(graph->keyAxis(), graph->valueAxis()); + mGraph = graph; + updatePosition(); + } else + qDebug() << Q_FUNC_INFO << "graph isn't in same QCustomPlot instance as this item"; + } else + { + mGraph = 0; + } +} + +/*! + Sets the key of the graph's data point the tracer will be positioned at. This is the only free + coordinate of a tracer when attached to a graph. + + Depending on \ref setInterpolating, the tracer will be either positioned on the data point + closest to \a key, or will stay exactly at \a key and interpolate the value linearly. + + \see setGraph, setInterpolating +*/ +void QCPItemTracer::setGraphKey(double key) +{ + mGraphKey = key; +} + +/*! + Sets whether the value of the graph's data points shall be interpolated, when positioning the + tracer. + + If \a enabled is set to false and a key is given with \ref setGraphKey, the tracer is placed on + the data point of the graph which is closest to the key, but which is not necessarily exactly + there. If \a enabled is true, the tracer will be positioned exactly at the specified key, and + the appropriate value will be interpolated from the graph's data points linearly. + + \see setGraph, setGraphKey +*/ +void QCPItemTracer::setInterpolating(bool enabled) +{ + mInterpolating = enabled; +} + +/* inherits documentation from base class */ +double QCPItemTracer::selectTest(const QPointF &pos, bool onlySelectable, QVariant *details) const +{ + Q_UNUSED(details) + if (onlySelectable && !mSelectable) + return -1; + + QPointF center(position->pixelPoint()); + double w = mSize/2.0; + QRect clip = clipRect(); + switch (mStyle) + { + case tsNone: return -1; + case tsPlus: + { + if (clipRect().intersects(QRectF(center-QPointF(w, w), center+QPointF(w, w)).toRect())) + return qSqrt(qMin(distSqrToLine(center+QPointF(-w, 0), center+QPointF(w, 0), pos), + distSqrToLine(center+QPointF(0, -w), center+QPointF(0, w), pos))); + break; + } + case tsCrosshair: + { + return qSqrt(qMin(distSqrToLine(QPointF(clip.left(), center.y()), QPointF(clip.right(), center.y()), pos), + distSqrToLine(QPointF(center.x(), clip.top()), QPointF(center.x(), clip.bottom()), pos))); + } + case tsCircle: + { + if (clip.intersects(QRectF(center-QPointF(w, w), center+QPointF(w, w)).toRect())) + { + // distance to border: + double centerDist = QVector2D(center-pos).length(); + double circleLine = w; + double result = qAbs(centerDist-circleLine); + // filled ellipse, allow click inside to count as hit: + if (result > mParentPlot->selectionTolerance()*0.99 && mBrush.style() != Qt::NoBrush && mBrush.color().alpha() != 0) + { + if (centerDist <= circleLine) + result = mParentPlot->selectionTolerance()*0.99; + } + return result; + } + break; + } + case tsSquare: + { + if (clip.intersects(QRectF(center-QPointF(w, w), center+QPointF(w, w)).toRect())) + { + QRectF rect = QRectF(center-QPointF(w, w), center+QPointF(w, w)); + bool filledRect = mBrush.style() != Qt::NoBrush && mBrush.color().alpha() != 0; + return rectSelectTest(rect, pos, filledRect); + } + break; + } + } + return -1; +} + +/* inherits documentation from base class */ +void QCPItemTracer::draw(QCPPainter *painter) +{ + updatePosition(); + if (mStyle == tsNone) + return; + + painter->setPen(mainPen()); + painter->setBrush(mainBrush()); + QPointF center(position->pixelPoint()); + double w = mSize/2.0; + QRect clip = clipRect(); + switch (mStyle) + { + case tsNone: return; + case tsPlus: + { + if (clip.intersects(QRectF(center-QPointF(w, w), center+QPointF(w, w)).toRect())) + { + painter->drawLine(QLineF(center+QPointF(-w, 0), center+QPointF(w, 0))); + painter->drawLine(QLineF(center+QPointF(0, -w), center+QPointF(0, w))); + } + break; + } + case tsCrosshair: + { + if (center.y() > clip.top() && center.y() < clip.bottom()) + painter->drawLine(QLineF(clip.left(), center.y(), clip.right(), center.y())); + if (center.x() > clip.left() && center.x() < clip.right()) + painter->drawLine(QLineF(center.x(), clip.top(), center.x(), clip.bottom())); + break; + } + case tsCircle: + { + if (clip.intersects(QRectF(center-QPointF(w, w), center+QPointF(w, w)).toRect())) + painter->drawEllipse(center, w, w); + break; + } + case tsSquare: + { + if (clip.intersects(QRectF(center-QPointF(w, w), center+QPointF(w, w)).toRect())) + painter->drawRect(QRectF(center-QPointF(w, w), center+QPointF(w, w))); + break; + } + } +} + +/*! + If the tracer is connected with a graph (\ref setGraph), this function updates the tracer's \a + position to reside on the graph data, depending on the configured key (\ref setGraphKey). + + It is called automatically on every redraw and normally doesn't need to be called manually. One + exception is when you want to read the tracer coordinates via \a position and are not sure that + the graph's data (or the tracer key with \ref setGraphKey) hasn't changed since the last redraw. + In that situation, call this function before accessing \a position, to make sure you don't get + out-of-date coordinates. + + If there is no graph set on this tracer, this function does nothing. +*/ +void QCPItemTracer::updatePosition() +{ + if (mGraph) + { + if (mParentPlot->hasPlottable(mGraph)) + { + if (mGraph->data()->size() > 1) + { + QCPDataMap::const_iterator first = mGraph->data()->constBegin(); + QCPDataMap::const_iterator last = mGraph->data()->constEnd()-1; + if (mGraphKey < first.key()) + position->setCoords(first.key(), first.value().value); + else if (mGraphKey > last.key()) + position->setCoords(last.key(), last.value().value); + else + { + QCPDataMap::const_iterator it = mGraph->data()->lowerBound(mGraphKey); + if (it != first) // mGraphKey is somewhere between iterators + { + QCPDataMap::const_iterator prevIt = it-1; + if (mInterpolating) + { + // interpolate between iterators around mGraphKey: + double slope = 0; + if (!qFuzzyCompare((double)it.key(), (double)prevIt.key())) + slope = (it.value().value-prevIt.value().value)/(it.key()-prevIt.key()); + position->setCoords(mGraphKey, (mGraphKey-prevIt.key())*slope+prevIt.value().value); + } else + { + // find iterator with key closest to mGraphKey: + if (mGraphKey < (prevIt.key()+it.key())*0.5) + it = prevIt; + position->setCoords(it.key(), it.value().value); + } + } else // mGraphKey is exactly on first iterator + position->setCoords(it.key(), it.value().value); + } + } else if (mGraph->data()->size() == 1) + { + QCPDataMap::const_iterator it = mGraph->data()->constBegin(); + position->setCoords(it.key(), it.value().value); + } else + qDebug() << Q_FUNC_INFO << "graph has no data"; + } else + qDebug() << Q_FUNC_INFO << "graph not contained in QCustomPlot instance (anymore)"; + } +} + +/*! \internal + + Returns the pen that should be used for drawing lines. Returns mPen when the item is not selected + and mSelectedPen when it is. +*/ +QPen QCPItemTracer::mainPen() const +{ + return mSelected ? mSelectedPen : mPen; +} + +/*! \internal + + Returns the brush that should be used for drawing fills of the item. Returns mBrush when the item + is not selected and mSelectedBrush when it is. +*/ +QBrush QCPItemTracer::mainBrush() const +{ + return mSelected ? mSelectedBrush : mBrush; +} + + +//////////////////////////////////////////////////////////////////////////////////////////////////// +//////////////////// QCPItemBracket +//////////////////////////////////////////////////////////////////////////////////////////////////// + +/*! \class QCPItemBracket + \brief A bracket for referencing/highlighting certain parts in the plot. + + \image html QCPItemBracket.png "Bracket example. Blue dotted circles are anchors, solid blue discs are positions." + + It has two positions, \a left and \a right, which define the span of the bracket. If \a left is + actually farther to the left than \a right, the bracket is opened to the bottom, as shown in the + example image. + + The bracket supports multiple styles via \ref setStyle. The length, i.e. how far the bracket + stretches away from the embraced span, can be controlled with \ref setLength. + + \image html QCPItemBracket-length.png +
Demonstrating the effect of different values for \ref setLength, for styles \ref + bsCalligraphic and \ref bsSquare. Anchors and positions are displayed for reference.
+ + It provides an anchor \a center, to allow connection of other items, e.g. an arrow (QCPItemLine + or QCPItemCurve) or a text label (QCPItemText), to the bracket. +*/ + +/*! + Creates a bracket item and sets default values. + + The constructed item can be added to the plot with QCustomPlot::addItem. +*/ +QCPItemBracket::QCPItemBracket(QCustomPlot *parentPlot) : + QCPAbstractItem(parentPlot), + left(createPosition(QLatin1String("left"))), + right(createPosition(QLatin1String("right"))), + center(createAnchor(QLatin1String("center"), aiCenter)) +{ + left->setCoords(0, 0); + right->setCoords(1, 1); + + setPen(QPen(Qt::black)); + setSelectedPen(QPen(Qt::blue, 2)); + setLength(8); + setStyle(bsCalligraphic); +} + +QCPItemBracket::~QCPItemBracket() +{ +} + +/*! + Sets the pen that will be used to draw the bracket. + + Note that when the style is \ref bsCalligraphic, only the color will be taken from the pen, the + stroke and width are ignored. To change the apparent stroke width of a calligraphic bracket, use + \ref setLength, which has a similar effect. + + \see setSelectedPen +*/ +void QCPItemBracket::setPen(const QPen &pen) +{ + mPen = pen; +} + +/*! + Sets the pen that will be used to draw the bracket when selected + + \see setPen, setSelected +*/ +void QCPItemBracket::setSelectedPen(const QPen &pen) +{ + mSelectedPen = pen; +} + +/*! + Sets the \a length in pixels how far the bracket extends in the direction towards the embraced + span of the bracket (i.e. perpendicular to the left-right-direction) + + \image html QCPItemBracket-length.png +
Demonstrating the effect of different values for \ref setLength, for styles \ref + bsCalligraphic and \ref bsSquare. Anchors and positions are displayed for reference.
+*/ +void QCPItemBracket::setLength(double length) +{ + mLength = length; +} + +/*! + Sets the style of the bracket, i.e. the shape/visual appearance. + + \see setPen +*/ +void QCPItemBracket::setStyle(QCPItemBracket::BracketStyle style) +{ + mStyle = style; +} + +/* inherits documentation from base class */ +double QCPItemBracket::selectTest(const QPointF &pos, bool onlySelectable, QVariant *details) const +{ + Q_UNUSED(details) + if (onlySelectable && !mSelectable) + return -1; + + QVector2D leftVec(left->pixelPoint()); + QVector2D rightVec(right->pixelPoint()); + if (leftVec.toPoint() == rightVec.toPoint()) + return -1; + + QVector2D widthVec = (rightVec-leftVec)*0.5f; + QVector2D lengthVec(-widthVec.y(), widthVec.x()); + lengthVec = lengthVec.normalized()*mLength; + QVector2D centerVec = (rightVec+leftVec)*0.5f-lengthVec; + + switch (mStyle) + { + case QCPItemBracket::bsSquare: + case QCPItemBracket::bsRound: + { + double a = distSqrToLine((centerVec-widthVec).toPointF(), (centerVec+widthVec).toPointF(), pos); + double b = distSqrToLine((centerVec-widthVec+lengthVec).toPointF(), (centerVec-widthVec).toPointF(), pos); + double c = distSqrToLine((centerVec+widthVec+lengthVec).toPointF(), (centerVec+widthVec).toPointF(), pos); + return qSqrt(qMin(qMin(a, b), c)); + } + case QCPItemBracket::bsCurly: + case QCPItemBracket::bsCalligraphic: + { + double a = distSqrToLine((centerVec-widthVec*0.75f+lengthVec*0.15f).toPointF(), (centerVec+lengthVec*0.3f).toPointF(), pos); + double b = distSqrToLine((centerVec-widthVec+lengthVec*0.7f).toPointF(), (centerVec-widthVec*0.75f+lengthVec*0.15f).toPointF(), pos); + double c = distSqrToLine((centerVec+widthVec*0.75f+lengthVec*0.15f).toPointF(), (centerVec+lengthVec*0.3f).toPointF(), pos); + double d = distSqrToLine((centerVec+widthVec+lengthVec*0.7f).toPointF(), (centerVec+widthVec*0.75f+lengthVec*0.15f).toPointF(), pos); + return qSqrt(qMin(qMin(a, b), qMin(c, d))); + } + } + return -1; +} + +/* inherits documentation from base class */ +void QCPItemBracket::draw(QCPPainter *painter) +{ + QVector2D leftVec(left->pixelPoint()); + QVector2D rightVec(right->pixelPoint()); + if (leftVec.toPoint() == rightVec.toPoint()) + return; + + QVector2D widthVec = (rightVec-leftVec)*0.5f; + QVector2D lengthVec(-widthVec.y(), widthVec.x()); + lengthVec = lengthVec.normalized()*mLength; + QVector2D centerVec = (rightVec+leftVec)*0.5f-lengthVec; + + QPolygon boundingPoly; + boundingPoly << leftVec.toPoint() << rightVec.toPoint() + << (rightVec-lengthVec).toPoint() << (leftVec-lengthVec).toPoint(); + QRect clip = clipRect().adjusted(-mainPen().widthF(), -mainPen().widthF(), mainPen().widthF(), mainPen().widthF()); + if (clip.intersects(boundingPoly.boundingRect())) + { + painter->setPen(mainPen()); + switch (mStyle) + { + case bsSquare: + { + painter->drawLine((centerVec+widthVec).toPointF(), (centerVec-widthVec).toPointF()); + painter->drawLine((centerVec+widthVec).toPointF(), (centerVec+widthVec+lengthVec).toPointF()); + painter->drawLine((centerVec-widthVec).toPointF(), (centerVec-widthVec+lengthVec).toPointF()); + break; + } + case bsRound: + { + painter->setBrush(Qt::NoBrush); + QPainterPath path; + path.moveTo((centerVec+widthVec+lengthVec).toPointF()); + path.cubicTo((centerVec+widthVec).toPointF(), (centerVec+widthVec).toPointF(), centerVec.toPointF()); + path.cubicTo((centerVec-widthVec).toPointF(), (centerVec-widthVec).toPointF(), (centerVec-widthVec+lengthVec).toPointF()); + painter->drawPath(path); + break; + } + case bsCurly: + { + painter->setBrush(Qt::NoBrush); + QPainterPath path; + path.moveTo((centerVec+widthVec+lengthVec).toPointF()); + path.cubicTo((centerVec+widthVec-lengthVec*0.8f).toPointF(), (centerVec+0.4f*widthVec+lengthVec).toPointF(), centerVec.toPointF()); + path.cubicTo((centerVec-0.4f*widthVec+lengthVec).toPointF(), (centerVec-widthVec-lengthVec*0.8f).toPointF(), (centerVec-widthVec+lengthVec).toPointF()); + painter->drawPath(path); + break; + } + case bsCalligraphic: + { + painter->setPen(Qt::NoPen); + painter->setBrush(QBrush(mainPen().color())); + QPainterPath path; + path.moveTo((centerVec+widthVec+lengthVec).toPointF()); + + path.cubicTo((centerVec+widthVec-lengthVec*0.8f).toPointF(), (centerVec+0.4f*widthVec+0.8f*lengthVec).toPointF(), centerVec.toPointF()); + path.cubicTo((centerVec-0.4f*widthVec+0.8f*lengthVec).toPointF(), (centerVec-widthVec-lengthVec*0.8f).toPointF(), (centerVec-widthVec+lengthVec).toPointF()); + + path.cubicTo((centerVec-widthVec-lengthVec*0.5f).toPointF(), (centerVec-0.2f*widthVec+1.2f*lengthVec).toPointF(), (centerVec+lengthVec*0.2f).toPointF()); + path.cubicTo((centerVec+0.2f*widthVec+1.2f*lengthVec).toPointF(), (centerVec+widthVec-lengthVec*0.5f).toPointF(), (centerVec+widthVec+lengthVec).toPointF()); + + painter->drawPath(path); + break; + } + } + } +} + +/* inherits documentation from base class */ +QPointF QCPItemBracket::anchorPixelPoint(int anchorId) const +{ + QVector2D leftVec(left->pixelPoint()); + QVector2D rightVec(right->pixelPoint()); + if (leftVec.toPoint() == rightVec.toPoint()) + return leftVec.toPointF(); + + QVector2D widthVec = (rightVec-leftVec)*0.5f; + QVector2D lengthVec(-widthVec.y(), widthVec.x()); + lengthVec = lengthVec.normalized()*mLength; + QVector2D centerVec = (rightVec+leftVec)*0.5f-lengthVec; + + switch (anchorId) + { + case aiCenter: + return centerVec.toPointF(); + } + qDebug() << Q_FUNC_INFO << "invalid anchorId" << anchorId; + return QPointF(); +} + +/*! \internal + + Returns the pen that should be used for drawing lines. Returns mPen when the + item is not selected and mSelectedPen when it is. +*/ +QPen QCPItemBracket::mainPen() const +{ + return mSelected ? mSelectedPen : mPen; +} + diff --git a/Analysis/DataViewer/qcustomplot.h b/Analysis/DataViewer/qcustomplot.h new file mode 100644 index 00000000..bb998f1c --- /dev/null +++ b/Analysis/DataViewer/qcustomplot.h @@ -0,0 +1,3768 @@ +/*************************************************************************** +** ** +** QCustomPlot, an easy to use, modern plotting widget for Qt ** +** Copyright (C) 2011-2015 Emanuel Eichhammer ** +** ** +** This program is free software: you can redistribute it and/or modify ** +** it under the terms of the GNU General Public License as published by ** +** the Free Software Foundation, either version 3 of the License, or ** +** (at your option) any later version. ** +** ** +** This program is distributed in the hope that it will be useful, ** +** but WITHOUT ANY WARRANTY; without even the implied warranty of ** +** MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the ** +** GNU General Public License for more details. ** +** ** +** You should have received a copy of the GNU General Public License ** +** along with this program. If not, see http://www.gnu.org/licenses/. ** +** ** +**************************************************************************** +** Author: Emanuel Eichhammer ** +** Website/Contact: http://www.qcustomplot.com/ ** +** Date: 22.12.15 ** +** Version: 1.3.2 ** +****************************************************************************/ + +#ifndef QCUSTOMPLOT_H +#define QCUSTOMPLOT_H + +#include +#include +#include +#include +#include +#include +#include +#include +#include +#include +#include +#include +#include +#include +#include +#include +#include +#include +#include +#if QT_VERSION < QT_VERSION_CHECK(5, 0, 0) +# include +# include +# include +#else +# include +# include +#endif + +class QCPPainter; +class QCustomPlot; +class QCPLayerable; +class QCPLayoutElement; +class QCPLayout; +class QCPAxis; +class QCPAxisRect; +class QCPAxisPainterPrivate; +class QCPAbstractPlottable; +class QCPGraph; +class QCPAbstractItem; +class QCPItemPosition; +class QCPLayer; +class QCPPlotTitle; +class QCPLegend; +class QCPAbstractLegendItem; +class QCPColorMap; +class QCPColorScale; +class QCPBars; + + +/*! \file */ + + +// decl definitions for shared library compilation/usage: +#if defined(QCUSTOMPLOT_COMPILE_LIBRARY) +# define QCP_LIB_DECL Q_DECL_EXPORT +#elif defined(QCUSTOMPLOT_USE_LIBRARY) +# define QCP_LIB_DECL Q_DECL_IMPORT +#else +# define QCP_LIB_DECL +#endif + +/*! + The QCP Namespace contains general enums and QFlags used throughout the QCustomPlot library +*/ +namespace QCP +{ +/*! + Defines the sides of a rectangular entity to which margins can be applied. + + \see QCPLayoutElement::setAutoMargins, QCPAxisRect::setAutoMargins +*/ +enum MarginSide { msLeft = 0x01 ///< 0x01 left margin + ,msRight = 0x02 ///< 0x02 right margin + ,msTop = 0x04 ///< 0x04 top margin + ,msBottom = 0x08 ///< 0x08 bottom margin + ,msAll = 0xFF ///< 0xFF all margins + ,msNone = 0x00 ///< 0x00 no margin + }; +Q_DECLARE_FLAGS(MarginSides, MarginSide) + +/*! + Defines what objects of a plot can be forcibly drawn antialiased/not antialiased. If an object is + neither forcibly drawn antialiased nor forcibly drawn not antialiased, it is up to the respective + element how it is drawn. Typically it provides a \a setAntialiased function for this. + + \c AntialiasedElements is a flag of or-combined elements of this enum type. + + \see QCustomPlot::setAntialiasedElements, QCustomPlot::setNotAntialiasedElements +*/ +enum AntialiasedElement { aeAxes = 0x0001 ///< 0x0001 Axis base line and tick marks + ,aeGrid = 0x0002 ///< 0x0002 Grid lines + ,aeSubGrid = 0x0004 ///< 0x0004 Sub grid lines + ,aeLegend = 0x0008 ///< 0x0008 Legend box + ,aeLegendItems = 0x0010 ///< 0x0010 Legend items + ,aePlottables = 0x0020 ///< 0x0020 Main lines of plottables (excluding error bars, see element \ref aeErrorBars) + ,aeItems = 0x0040 ///< 0x0040 Main lines of items + ,aeScatters = 0x0080 ///< 0x0080 Scatter symbols of plottables (excluding scatter symbols of type ssPixmap) + ,aeErrorBars = 0x0100 ///< 0x0100 Error bars + ,aeFills = 0x0200 ///< 0x0200 Borders of fills (e.g. under or between graphs) + ,aeZeroLine = 0x0400 ///< 0x0400 Zero-lines, see \ref QCPGrid::setZeroLinePen + ,aeAll = 0xFFFF ///< 0xFFFF All elements + ,aeNone = 0x0000 ///< 0x0000 No elements + }; +Q_DECLARE_FLAGS(AntialiasedElements, AntialiasedElement) + +/*! + Defines plotting hints that control various aspects of the quality and speed of plotting. + + \see QCustomPlot::setPlottingHints +*/ +enum PlottingHint { phNone = 0x000 ///< 0x000 No hints are set + ,phFastPolylines = 0x001 ///< 0x001 Graph/Curve lines are drawn with a faster method. This reduces the quality + ///< especially of the line segment joins. (Only relevant for solid line pens.) + ,phForceRepaint = 0x002 ///< 0x002 causes an immediate repaint() instead of a soft update() when QCustomPlot::replot() is called with parameter \ref QCustomPlot::rpHint. + ///< This is set by default to prevent the plot from freezing on fast consecutive replots (e.g. user drags ranges with mouse). + ,phCacheLabels = 0x004 ///< 0x004 axis (tick) labels will be cached as pixmaps, increasing replot performance. + }; +Q_DECLARE_FLAGS(PlottingHints, PlottingHint) + +/*! + Defines the mouse interactions possible with QCustomPlot. + + \c Interactions is a flag of or-combined elements of this enum type. + + \see QCustomPlot::setInteractions +*/ +enum Interaction { iRangeDrag = 0x001 ///< 0x001 Axis ranges are draggable (see \ref QCPAxisRect::setRangeDrag, \ref QCPAxisRect::setRangeDragAxes) + ,iRangeZoom = 0x002 ///< 0x002 Axis ranges are zoomable with the mouse wheel (see \ref QCPAxisRect::setRangeZoom, \ref QCPAxisRect::setRangeZoomAxes) + ,iMultiSelect = 0x004 ///< 0x004 The user can select multiple objects by holding the modifier set by \ref QCustomPlot::setMultiSelectModifier while clicking + ,iSelectPlottables = 0x008 ///< 0x008 Plottables are selectable (e.g. graphs, curves, bars,... see QCPAbstractPlottable) + ,iSelectAxes = 0x010 ///< 0x010 Axes are selectable (or parts of them, see QCPAxis::setSelectableParts) + ,iSelectLegend = 0x020 ///< 0x020 Legends are selectable (or their child items, see QCPLegend::setSelectableParts) + ,iSelectItems = 0x040 ///< 0x040 Items are selectable (Rectangles, Arrows, Textitems, etc. see \ref QCPAbstractItem) + ,iSelectOther = 0x080 ///< 0x080 All other objects are selectable (e.g. your own derived layerables, the plot title,...) + }; +Q_DECLARE_FLAGS(Interactions, Interaction) + +/*! \internal + + Returns whether the specified \a value is considered an invalid data value for plottables (i.e. + is \e nan or \e +/-inf). This function is used to check data validity upon replots, when the + compiler flag \c QCUSTOMPLOT_CHECK_DATA is set. +*/ +inline bool isInvalidData(double value) +{ + return qIsNaN(value) || qIsInf(value); +} + +/*! \internal + \overload + + Checks two arguments instead of one. +*/ +inline bool isInvalidData(double value1, double value2) +{ + return isInvalidData(value1) || isInvalidData(value2); +} + +/*! \internal + + Sets the specified \a side of \a margins to \a value + + \see getMarginValue +*/ +inline void setMarginValue(QMargins &margins, QCP::MarginSide side, int value) +{ + switch (side) + { + case QCP::msLeft: margins.setLeft(value); break; + case QCP::msRight: margins.setRight(value); break; + case QCP::msTop: margins.setTop(value); break; + case QCP::msBottom: margins.setBottom(value); break; + case QCP::msAll: margins = QMargins(value, value, value, value); break; + default: break; + } +} + +/*! \internal + + Returns the value of the specified \a side of \a margins. If \a side is \ref QCP::msNone or + \ref QCP::msAll, returns 0. + + \see setMarginValue +*/ +inline int getMarginValue(const QMargins &margins, QCP::MarginSide side) +{ + switch (side) + { + case QCP::msLeft: return margins.left(); + case QCP::msRight: return margins.right(); + case QCP::msTop: return margins.top(); + case QCP::msBottom: return margins.bottom(); + default: break; + } + return 0; +} + +} // end of namespace QCP + +Q_DECLARE_OPERATORS_FOR_FLAGS(QCP::AntialiasedElements) +Q_DECLARE_OPERATORS_FOR_FLAGS(QCP::PlottingHints) +Q_DECLARE_OPERATORS_FOR_FLAGS(QCP::MarginSides) +Q_DECLARE_OPERATORS_FOR_FLAGS(QCP::Interactions) + + +class QCP_LIB_DECL QCPScatterStyle +{ + Q_GADGET +public: + /*! + Defines the shape used for scatter points. + + On plottables/items that draw scatters, the sizes of these visualizations (with exception of + \ref ssDot and \ref ssPixmap) can be controlled with the \ref setSize function. Scatters are + drawn with the pen and brush specified with \ref setPen and \ref setBrush. + */ + Q_ENUMS(ScatterShape) + enum ScatterShape { ssNone ///< no scatter symbols are drawn (e.g. in QCPGraph, data only represented with lines) + ,ssDot ///< \enumimage{ssDot.png} a single pixel (use \ref ssDisc or \ref ssCircle if you want a round shape with a certain radius) + ,ssCross ///< \enumimage{ssCross.png} a cross + ,ssPlus ///< \enumimage{ssPlus.png} a plus + ,ssCircle ///< \enumimage{ssCircle.png} a circle + ,ssDisc ///< \enumimage{ssDisc.png} a circle which is filled with the pen's color (not the brush as with ssCircle) + ,ssSquare ///< \enumimage{ssSquare.png} a square + ,ssDiamond ///< \enumimage{ssDiamond.png} a diamond + ,ssStar ///< \enumimage{ssStar.png} a star with eight arms, i.e. a combination of cross and plus + ,ssTriangle ///< \enumimage{ssTriangle.png} an equilateral triangle, standing on baseline + ,ssTriangleInverted ///< \enumimage{ssTriangleInverted.png} an equilateral triangle, standing on corner + ,ssCrossSquare ///< \enumimage{ssCrossSquare.png} a square with a cross inside + ,ssPlusSquare ///< \enumimage{ssPlusSquare.png} a square with a plus inside + ,ssCrossCircle ///< \enumimage{ssCrossCircle.png} a circle with a cross inside + ,ssPlusCircle ///< \enumimage{ssPlusCircle.png} a circle with a plus inside + ,ssPeace ///< \enumimage{ssPeace.png} a circle, with one vertical and two downward diagonal lines + ,ssPixmap ///< a custom pixmap specified by \ref setPixmap, centered on the data point coordinates + ,ssCustom ///< custom painter operations are performed per scatter (As QPainterPath, see \ref setCustomPath) + }; + + QCPScatterStyle(); + QCPScatterStyle(ScatterShape shape, double size=6); + QCPScatterStyle(ScatterShape shape, const QColor &color, double size); + QCPScatterStyle(ScatterShape shape, const QColor &color, const QColor &fill, double size); + QCPScatterStyle(ScatterShape shape, const QPen &pen, const QBrush &brush, double size); + QCPScatterStyle(const QPixmap &pixmap); + QCPScatterStyle(const QPainterPath &customPath, const QPen &pen, const QBrush &brush=Qt::NoBrush, double size=6); + + // getters: + double size() const { return mSize; } + ScatterShape shape() const { return mShape; } + QPen pen() const { return mPen; } + QBrush brush() const { return mBrush; } + QPixmap pixmap() const { return mPixmap; } + QPainterPath customPath() const { return mCustomPath; } + + // setters: + void setSize(double size); + void setShape(ScatterShape shape); + void setPen(const QPen &pen); + void setBrush(const QBrush &brush); + void setPixmap(const QPixmap &pixmap); + void setCustomPath(const QPainterPath &customPath); + + // non-property methods: + bool isNone() const { return mShape == ssNone; } + bool isPenDefined() const { return mPenDefined; } + void applyTo(QCPPainter *painter, const QPen &defaultPen) const; + void drawShape(QCPPainter *painter, QPointF pos) const; + void drawShape(QCPPainter *painter, double x, double y) const; + +protected: + // property members: + double mSize; + ScatterShape mShape; + QPen mPen; + QBrush mBrush; + QPixmap mPixmap; + QPainterPath mCustomPath; + + // non-property members: + bool mPenDefined; +}; +Q_DECLARE_TYPEINFO(QCPScatterStyle, Q_MOVABLE_TYPE); + + +class QCP_LIB_DECL QCPPainter : public QPainter +{ + Q_GADGET +public: + /*! + Defines special modes the painter can operate in. They disable or enable certain subsets of features/fixes/workarounds, + depending on whether they are wanted on the respective output device. + */ + enum PainterMode { pmDefault = 0x00 ///< 0x00 Default mode for painting on screen devices + ,pmVectorized = 0x01 ///< 0x01 Mode for vectorized painting (e.g. PDF export). For example, this prevents some antialiasing fixes. + ,pmNoCaching = 0x02 ///< 0x02 Mode for all sorts of exports (e.g. PNG, PDF,...). For example, this prevents using cached pixmap labels + ,pmNonCosmetic = 0x04 ///< 0x04 Turns pen widths 0 to 1, i.e. disables cosmetic pens. (A cosmetic pen is always drawn with width 1 pixel in the vector image/pdf viewer, independent of zoom.) + }; + Q_FLAGS(PainterMode PainterModes) + Q_DECLARE_FLAGS(PainterModes, PainterMode) + + QCPPainter(); + QCPPainter(QPaintDevice *device); + ~QCPPainter(); + + // getters: + bool antialiasing() const { return testRenderHint(QPainter::Antialiasing); } + PainterModes modes() const { return mModes; } + + // setters: + void setAntialiasing(bool enabled); + void setMode(PainterMode mode, bool enabled=true); + void setModes(PainterModes modes); + + // methods hiding non-virtual base class functions (QPainter bug workarounds): + bool begin(QPaintDevice *device); + void setPen(const QPen &pen); + void setPen(const QColor &color); + void setPen(Qt::PenStyle penStyle); + void drawLine(const QLineF &line); + void drawLine(const QPointF &p1, const QPointF &p2) {drawLine(QLineF(p1, p2));} + void save(); + void restore(); + + // non-virtual methods: + void makeNonCosmetic(); + +protected: + // property members: + PainterModes mModes; + bool mIsAntialiasing; + + // non-property members: + QStack mAntialiasingStack; +}; +Q_DECLARE_OPERATORS_FOR_FLAGS(QCPPainter::PainterModes) + + +class QCP_LIB_DECL QCPLayer : public QObject +{ + Q_OBJECT + /// \cond INCLUDE_QPROPERTIES + Q_PROPERTY(QCustomPlot* parentPlot READ parentPlot) + Q_PROPERTY(QString name READ name) + Q_PROPERTY(int index READ index) + Q_PROPERTY(QList children READ children) + Q_PROPERTY(bool visible READ visible WRITE setVisible) + /// \endcond +public: + QCPLayer(QCustomPlot* parentPlot, const QString &layerName); + ~QCPLayer(); + + // getters: + QCustomPlot *parentPlot() const { return mParentPlot; } + QString name() const { return mName; } + int index() const { return mIndex; } + QList children() const { return mChildren; } + bool visible() const { return mVisible; } + + // setters: + void setVisible(bool visible); + +protected: + // property members: + QCustomPlot *mParentPlot; + QString mName; + int mIndex; + QList mChildren; + bool mVisible; + + // non-virtual methods: + void addChild(QCPLayerable *layerable, bool prepend); + void removeChild(QCPLayerable *layerable); + +private: + Q_DISABLE_COPY(QCPLayer) + + friend class QCustomPlot; + friend class QCPLayerable; +}; + +class QCP_LIB_DECL QCPLayerable : public QObject +{ + Q_OBJECT + /// \cond INCLUDE_QPROPERTIES + Q_PROPERTY(bool visible READ visible WRITE setVisible) + Q_PROPERTY(QCustomPlot* parentPlot READ parentPlot) + Q_PROPERTY(QCPLayerable* parentLayerable READ parentLayerable) + Q_PROPERTY(QCPLayer* layer READ layer WRITE setLayer NOTIFY layerChanged) + Q_PROPERTY(bool antialiased READ antialiased WRITE setAntialiased) + /// \endcond +public: + QCPLayerable(QCustomPlot *plot, QString targetLayer=QString(), QCPLayerable *parentLayerable=0); + ~QCPLayerable(); + + // getters: + bool visible() const { return mVisible; } + QCustomPlot *parentPlot() const { return mParentPlot; } + QCPLayerable *parentLayerable() const { return mParentLayerable.data(); } + QCPLayer *layer() const { return mLayer; } + bool antialiased() const { return mAntialiased; } + + // setters: + void setVisible(bool on); + Q_SLOT bool setLayer(QCPLayer *layer); + bool setLayer(const QString &layerName); + void setAntialiased(bool enabled); + + // introduced virtual methods: + virtual double selectTest(const QPointF &pos, bool onlySelectable, QVariant *details=0) const; + + // non-property methods: + bool realVisibility() const; + +signals: + void layerChanged(QCPLayer *newLayer); + +protected: + // property members: + bool mVisible; + QCustomPlot *mParentPlot; + QPointer mParentLayerable; + QCPLayer *mLayer; + bool mAntialiased; + + // introduced virtual methods: + virtual void parentPlotInitialized(QCustomPlot *parentPlot); + virtual QCP::Interaction selectionCategory() const; + virtual QRect clipRect() const; + virtual void applyDefaultAntialiasingHint(QCPPainter *painter) const = 0; + virtual void draw(QCPPainter *painter) = 0; + // events: + virtual void selectEvent(QMouseEvent *event, bool additive, const QVariant &details, bool *selectionStateChanged); + virtual void deselectEvent(bool *selectionStateChanged); + + // non-property methods: + void initializeParentPlot(QCustomPlot *parentPlot); + void setParentLayerable(QCPLayerable* parentLayerable); + bool moveToLayer(QCPLayer *layer, bool prepend); + void applyAntialiasingHint(QCPPainter *painter, bool localAntialiased, QCP::AntialiasedElement overrideElement) const; + +private: + Q_DISABLE_COPY(QCPLayerable) + + friend class QCustomPlot; + friend class QCPAxisRect; +}; + + +class QCP_LIB_DECL QCPRange +{ +public: + double lower, upper; + + QCPRange(); + QCPRange(double lower, double upper); + + bool operator==(const QCPRange& other) const { return lower == other.lower && upper == other.upper; } + bool operator!=(const QCPRange& other) const { return !(*this == other); } + + QCPRange &operator+=(const double& value) { lower+=value; upper+=value; return *this; } + QCPRange &operator-=(const double& value) { lower-=value; upper-=value; return *this; } + QCPRange &operator*=(const double& value) { lower*=value; upper*=value; return *this; } + QCPRange &operator/=(const double& value) { lower/=value; upper/=value; return *this; } + friend inline const QCPRange operator+(const QCPRange&, double); + friend inline const QCPRange operator+(double, const QCPRange&); + friend inline const QCPRange operator-(const QCPRange& range, double value); + friend inline const QCPRange operator*(const QCPRange& range, double value); + friend inline const QCPRange operator*(double value, const QCPRange& range); + friend inline const QCPRange operator/(const QCPRange& range, double value); + + double size() const; + double center() const; + void normalize(); + void expand(const QCPRange &otherRange); + QCPRange expanded(const QCPRange &otherRange) const; + QCPRange sanitizedForLogScale() const; + QCPRange sanitizedForLinScale() const; + bool contains(double value) const; + + static bool validRange(double lower, double upper); + static bool validRange(const QCPRange &range); + static const double minRange; //1e-280; + static const double maxRange; //1e280; + +}; +Q_DECLARE_TYPEINFO(QCPRange, Q_MOVABLE_TYPE); + +/* documentation of inline functions */ + +/*! \fn QCPRange &QCPRange::operator+=(const double& value) + + Adds \a value to both boundaries of the range. +*/ + +/*! \fn QCPRange &QCPRange::operator-=(const double& value) + + Subtracts \a value from both boundaries of the range. +*/ + +/*! \fn QCPRange &QCPRange::operator*=(const double& value) + + Multiplies both boundaries of the range by \a value. +*/ + +/*! \fn QCPRange &QCPRange::operator/=(const double& value) + + Divides both boundaries of the range by \a value. +*/ + +/* end documentation of inline functions */ + +/*! + Adds \a value to both boundaries of the range. +*/ +inline const QCPRange operator+(const QCPRange& range, double value) +{ + QCPRange result(range); + result += value; + return result; +} + +/*! + Adds \a value to both boundaries of the range. +*/ +inline const QCPRange operator+(double value, const QCPRange& range) +{ + QCPRange result(range); + result += value; + return result; +} + +/*! + Subtracts \a value from both boundaries of the range. +*/ +inline const QCPRange operator-(const QCPRange& range, double value) +{ + QCPRange result(range); + result -= value; + return result; +} + +/*! + Multiplies both boundaries of the range by \a value. +*/ +inline const QCPRange operator*(const QCPRange& range, double value) +{ + QCPRange result(range); + result *= value; + return result; +} + +/*! + Multiplies both boundaries of the range by \a value. +*/ +inline const QCPRange operator*(double value, const QCPRange& range) +{ + QCPRange result(range); + result *= value; + return result; +} + +/*! + Divides both boundaries of the range by \a value. +*/ +inline const QCPRange operator/(const QCPRange& range, double value) +{ + QCPRange result(range); + result /= value; + return result; +} + + +class QCP_LIB_DECL QCPMarginGroup : public QObject +{ + Q_OBJECT +public: + QCPMarginGroup(QCustomPlot *parentPlot); + ~QCPMarginGroup(); + + // non-virtual methods: + QList elements(QCP::MarginSide side) const { return mChildren.value(side); } + bool isEmpty() const; + void clear(); + +protected: + // non-property members: + QCustomPlot *mParentPlot; + QHash > mChildren; + + // non-virtual methods: + int commonMargin(QCP::MarginSide side) const; + void addChild(QCP::MarginSide side, QCPLayoutElement *element); + void removeChild(QCP::MarginSide side, QCPLayoutElement *element); + +private: + Q_DISABLE_COPY(QCPMarginGroup) + + friend class QCPLayoutElement; +}; + + +class QCP_LIB_DECL QCPLayoutElement : public QCPLayerable +{ + Q_OBJECT + /// \cond INCLUDE_QPROPERTIES + Q_PROPERTY(QCPLayout* layout READ layout) + Q_PROPERTY(QRect rect READ rect) + Q_PROPERTY(QRect outerRect READ outerRect WRITE setOuterRect) + Q_PROPERTY(QMargins margins READ margins WRITE setMargins) + Q_PROPERTY(QMargins minimumMargins READ minimumMargins WRITE setMinimumMargins) + Q_PROPERTY(QSize minimumSize READ minimumSize WRITE setMinimumSize) + Q_PROPERTY(QSize maximumSize READ maximumSize WRITE setMaximumSize) + /// \endcond +public: + /*! + Defines the phases of the update process, that happens just before a replot. At each phase, + \ref update is called with the according UpdatePhase value. + */ + enum UpdatePhase { upPreparation ///< Phase used for any type of preparation that needs to be done before margin calculation and layout + ,upMargins ///< Phase in which the margins are calculated and set + ,upLayout ///< Final phase in which the layout system places the rects of the elements + }; + Q_ENUMS(UpdatePhase) + + explicit QCPLayoutElement(QCustomPlot *parentPlot=0); + virtual ~QCPLayoutElement(); + + // getters: + QCPLayout *layout() const { return mParentLayout; } + QRect rect() const { return mRect; } + QRect outerRect() const { return mOuterRect; } + QMargins margins() const { return mMargins; } + QMargins minimumMargins() const { return mMinimumMargins; } + QCP::MarginSides autoMargins() const { return mAutoMargins; } + QSize minimumSize() const { return mMinimumSize; } + QSize maximumSize() const { return mMaximumSize; } + QCPMarginGroup *marginGroup(QCP::MarginSide side) const { return mMarginGroups.value(side, (QCPMarginGroup*)0); } + QHash marginGroups() const { return mMarginGroups; } + + // setters: + void setOuterRect(const QRect &rect); + void setMargins(const QMargins &margins); + void setMinimumMargins(const QMargins &margins); + void setAutoMargins(QCP::MarginSides sides); + void setMinimumSize(const QSize &size); + void setMinimumSize(int width, int height); + void setMaximumSize(const QSize &size); + void setMaximumSize(int width, int height); + void setMarginGroup(QCP::MarginSides sides, QCPMarginGroup *group); + + // introduced virtual methods: + virtual void update(UpdatePhase phase); + virtual QSize minimumSizeHint() const; + virtual QSize maximumSizeHint() const; + virtual QList elements(bool recursive) const; + + // reimplemented virtual methods: + virtual double selectTest(const QPointF &pos, bool onlySelectable, QVariant *details=0) const; + +protected: + // property members: + QCPLayout *mParentLayout; + QSize mMinimumSize, mMaximumSize; + QRect mRect, mOuterRect; + QMargins mMargins, mMinimumMargins; + QCP::MarginSides mAutoMargins; + QHash mMarginGroups; + + // introduced virtual methods: + virtual int calculateAutoMargin(QCP::MarginSide side); + // events: + virtual void mousePressEvent(QMouseEvent *event) {Q_UNUSED(event)} + virtual void mouseMoveEvent(QMouseEvent *event) {Q_UNUSED(event)} + virtual void mouseReleaseEvent(QMouseEvent *event) {Q_UNUSED(event)} + virtual void mouseDoubleClickEvent(QMouseEvent *event) {Q_UNUSED(event)} + virtual void wheelEvent(QWheelEvent *event) {Q_UNUSED(event)} + + // reimplemented virtual methods: + virtual void applyDefaultAntialiasingHint(QCPPainter *painter) const { Q_UNUSED(painter) } + virtual void draw(QCPPainter *painter) { Q_UNUSED(painter) } + virtual void parentPlotInitialized(QCustomPlot *parentPlot); + +private: + Q_DISABLE_COPY(QCPLayoutElement) + + friend class QCustomPlot; + friend class QCPLayout; + friend class QCPMarginGroup; +}; + + +class QCP_LIB_DECL QCPLayout : public QCPLayoutElement +{ + Q_OBJECT +public: + explicit QCPLayout(); + + // reimplemented virtual methods: + virtual void update(UpdatePhase phase); + virtual QList elements(bool recursive) const; + + // introduced virtual methods: + virtual int elementCount() const = 0; + virtual QCPLayoutElement* elementAt(int index) const = 0; + virtual QCPLayoutElement* takeAt(int index) = 0; + virtual bool take(QCPLayoutElement* element) = 0; + virtual void simplify(); + + // non-virtual methods: + bool removeAt(int index); + bool remove(QCPLayoutElement* element); + void clear(); + +protected: + // introduced virtual methods: + virtual void updateLayout(); + + // non-virtual methods: + void sizeConstraintsChanged() const; + void adoptElement(QCPLayoutElement *el); + void releaseElement(QCPLayoutElement *el); + QVector getSectionSizes(QVector maxSizes, QVector minSizes, QVector stretchFactors, int totalSize) const; + +private: + Q_DISABLE_COPY(QCPLayout) + friend class QCPLayoutElement; +}; + + +class QCP_LIB_DECL QCPLayoutGrid : public QCPLayout +{ + Q_OBJECT + /// \cond INCLUDE_QPROPERTIES + Q_PROPERTY(int rowCount READ rowCount) + Q_PROPERTY(int columnCount READ columnCount) + Q_PROPERTY(QList columnStretchFactors READ columnStretchFactors WRITE setColumnStretchFactors) + Q_PROPERTY(QList rowStretchFactors READ rowStretchFactors WRITE setRowStretchFactors) + Q_PROPERTY(int columnSpacing READ columnSpacing WRITE setColumnSpacing) + Q_PROPERTY(int rowSpacing READ rowSpacing WRITE setRowSpacing) + /// \endcond +public: + explicit QCPLayoutGrid(); + virtual ~QCPLayoutGrid(); + + // getters: + int rowCount() const; + int columnCount() const; + QList columnStretchFactors() const { return mColumnStretchFactors; } + QList rowStretchFactors() const { return mRowStretchFactors; } + int columnSpacing() const { return mColumnSpacing; } + int rowSpacing() const { return mRowSpacing; } + + // setters: + void setColumnStretchFactor(int column, double factor); + void setColumnStretchFactors(const QList &factors); + void setRowStretchFactor(int row, double factor); + void setRowStretchFactors(const QList &factors); + void setColumnSpacing(int pixels); + void setRowSpacing(int pixels); + + // reimplemented virtual methods: + virtual void updateLayout(); + virtual int elementCount() const; + virtual QCPLayoutElement* elementAt(int index) const; + virtual QCPLayoutElement* takeAt(int index); + virtual bool take(QCPLayoutElement* element); + virtual QList elements(bool recursive) const; + virtual void simplify(); + virtual QSize minimumSizeHint() const; + virtual QSize maximumSizeHint() const; + + // non-virtual methods: + QCPLayoutElement *element(int row, int column) const; + bool addElement(int row, int column, QCPLayoutElement *element); + bool hasElement(int row, int column); + void expandTo(int newRowCount, int newColumnCount); + void insertRow(int newIndex); + void insertColumn(int newIndex); + +protected: + // property members: + QList > mElements; + QList mColumnStretchFactors; + QList mRowStretchFactors; + int mColumnSpacing, mRowSpacing; + + // non-virtual methods: + void getMinimumRowColSizes(QVector *minColWidths, QVector *minRowHeights) const; + void getMaximumRowColSizes(QVector *maxColWidths, QVector *maxRowHeights) const; + +private: + Q_DISABLE_COPY(QCPLayoutGrid) +}; + + +class QCP_LIB_DECL QCPLayoutInset : public QCPLayout +{ + Q_OBJECT +public: + /*! + Defines how the placement and sizing is handled for a certain element in a QCPLayoutInset. + */ + enum InsetPlacement { ipFree ///< The element may be positioned/sized arbitrarily, see \ref setInsetRect + ,ipBorderAligned ///< The element is aligned to one of the layout sides, see \ref setInsetAlignment + }; + + explicit QCPLayoutInset(); + virtual ~QCPLayoutInset(); + + // getters: + InsetPlacement insetPlacement(int index) const; + Qt::Alignment insetAlignment(int index) const; + QRectF insetRect(int index) const; + + // setters: + void setInsetPlacement(int index, InsetPlacement placement); + void setInsetAlignment(int index, Qt::Alignment alignment); + void setInsetRect(int index, const QRectF &rect); + + // reimplemented virtual methods: + virtual void updateLayout(); + virtual int elementCount() const; + virtual QCPLayoutElement* elementAt(int index) const; + virtual QCPLayoutElement* takeAt(int index); + virtual bool take(QCPLayoutElement* element); + virtual void simplify() {} + virtual double selectTest(const QPointF &pos, bool onlySelectable, QVariant *details=0) const; + + // non-virtual methods: + void addElement(QCPLayoutElement *element, Qt::Alignment alignment); + void addElement(QCPLayoutElement *element, const QRectF &rect); + +protected: + // property members: + QList mElements; + QList mInsetPlacement; + QList mInsetAlignment; + QList mInsetRect; + +private: + Q_DISABLE_COPY(QCPLayoutInset) +}; + + +class QCP_LIB_DECL QCPLineEnding +{ + Q_GADGET +public: + /*! + Defines the type of ending decoration for line-like items, e.g. an arrow. + + \image html QCPLineEnding.png + + The width and length of these decorations can be controlled with the functions \ref setWidth + and \ref setLength. Some decorations like \ref esDisc, \ref esSquare, \ref esDiamond and \ref esBar only + support a width, the length property is ignored. + + \see QCPItemLine::setHead, QCPItemLine::setTail, QCPItemCurve::setHead, QCPItemCurve::setTail, QCPAxis::setLowerEnding, QCPAxis::setUpperEnding + */ + Q_ENUMS(EndingStyle) + enum EndingStyle { esNone ///< No ending decoration + ,esFlatArrow ///< A filled arrow head with a straight/flat back (a triangle) + ,esSpikeArrow ///< A filled arrow head with an indented back + ,esLineArrow ///< A non-filled arrow head with open back + ,esDisc ///< A filled circle + ,esSquare ///< A filled square + ,esDiamond ///< A filled diamond (45° rotated square) + ,esBar ///< A bar perpendicular to the line + ,esHalfBar ///< A bar perpendicular to the line, pointing out to only one side (to which side can be changed with \ref setInverted) + ,esSkewedBar ///< A bar that is skewed (skew controllable via \ref setLength) + }; + + QCPLineEnding(); + QCPLineEnding(EndingStyle style, double width=8, double length=10, bool inverted=false); + + // getters: + EndingStyle style() const { return mStyle; } + double width() const { return mWidth; } + double length() const { return mLength; } + bool inverted() const { return mInverted; } + + // setters: + void setStyle(EndingStyle style); + void setWidth(double width); + void setLength(double length); + void setInverted(bool inverted); + + // non-property methods: + double boundingDistance() const; + double realLength() const; + void draw(QCPPainter *painter, const QVector2D &pos, const QVector2D &dir) const; + void draw(QCPPainter *painter, const QVector2D &pos, double angle) const; + +protected: + // property members: + EndingStyle mStyle; + double mWidth, mLength; + bool mInverted; +}; +Q_DECLARE_TYPEINFO(QCPLineEnding, Q_MOVABLE_TYPE); + + +class QCP_LIB_DECL QCPGrid :public QCPLayerable +{ + Q_OBJECT + /// \cond INCLUDE_QPROPERTIES + Q_PROPERTY(bool subGridVisible READ subGridVisible WRITE setSubGridVisible) + Q_PROPERTY(bool antialiasedSubGrid READ antialiasedSubGrid WRITE setAntialiasedSubGrid) + Q_PROPERTY(bool antialiasedZeroLine READ antialiasedZeroLine WRITE setAntialiasedZeroLine) + Q_PROPERTY(QPen pen READ pen WRITE setPen) + Q_PROPERTY(QPen subGridPen READ subGridPen WRITE setSubGridPen) + Q_PROPERTY(QPen zeroLinePen READ zeroLinePen WRITE setZeroLinePen) + /// \endcond +public: + QCPGrid(QCPAxis *parentAxis); + + // getters: + bool subGridVisible() const { return mSubGridVisible; } + bool antialiasedSubGrid() const { return mAntialiasedSubGrid; } + bool antialiasedZeroLine() const { return mAntialiasedZeroLine; } + QPen pen() const { return mPen; } + QPen subGridPen() const { return mSubGridPen; } + QPen zeroLinePen() const { return mZeroLinePen; } + + // setters: + void setSubGridVisible(bool visible); + void setAntialiasedSubGrid(bool enabled); + void setAntialiasedZeroLine(bool enabled); + void setPen(const QPen &pen); + void setSubGridPen(const QPen &pen); + void setZeroLinePen(const QPen &pen); + +protected: + // property members: + bool mSubGridVisible; + bool mAntialiasedSubGrid, mAntialiasedZeroLine; + QPen mPen, mSubGridPen, mZeroLinePen; + // non-property members: + QCPAxis *mParentAxis; + + // reimplemented virtual methods: + virtual void applyDefaultAntialiasingHint(QCPPainter *painter) const; + virtual void draw(QCPPainter *painter); + + // non-virtual methods: + void drawGridLines(QCPPainter *painter) const; + void drawSubGridLines(QCPPainter *painter) const; + + friend class QCPAxis; +}; + + +class QCP_LIB_DECL QCPAxis : public QCPLayerable +{ + Q_OBJECT + /// \cond INCLUDE_QPROPERTIES + Q_PROPERTY(AxisType axisType READ axisType) + Q_PROPERTY(QCPAxisRect* axisRect READ axisRect) + Q_PROPERTY(ScaleType scaleType READ scaleType WRITE setScaleType NOTIFY scaleTypeChanged) + Q_PROPERTY(double scaleLogBase READ scaleLogBase WRITE setScaleLogBase) + Q_PROPERTY(QCPRange range READ range WRITE setRange NOTIFY rangeChanged) + Q_PROPERTY(bool rangeReversed READ rangeReversed WRITE setRangeReversed) + Q_PROPERTY(bool autoTicks READ autoTicks WRITE setAutoTicks) + Q_PROPERTY(int autoTickCount READ autoTickCount WRITE setAutoTickCount) + Q_PROPERTY(bool autoTickLabels READ autoTickLabels WRITE setAutoTickLabels) + Q_PROPERTY(bool autoTickStep READ autoTickStep WRITE setAutoTickStep) + Q_PROPERTY(bool autoSubTicks READ autoSubTicks WRITE setAutoSubTicks) + Q_PROPERTY(bool ticks READ ticks WRITE setTicks) + Q_PROPERTY(bool tickLabels READ tickLabels WRITE setTickLabels) + Q_PROPERTY(int tickLabelPadding READ tickLabelPadding WRITE setTickLabelPadding) + Q_PROPERTY(LabelType tickLabelType READ tickLabelType WRITE setTickLabelType) + Q_PROPERTY(QFont tickLabelFont READ tickLabelFont WRITE setTickLabelFont) + Q_PROPERTY(QColor tickLabelColor READ tickLabelColor WRITE setTickLabelColor) + Q_PROPERTY(double tickLabelRotation READ tickLabelRotation WRITE setTickLabelRotation) + Q_PROPERTY(LabelSide tickLabelSide READ tickLabelSide WRITE setTickLabelSide) + Q_PROPERTY(QString dateTimeFormat READ dateTimeFormat WRITE setDateTimeFormat) + Q_PROPERTY(Qt::TimeSpec dateTimeSpec READ dateTimeSpec WRITE setDateTimeSpec) + Q_PROPERTY(QString numberFormat READ numberFormat WRITE setNumberFormat) + Q_PROPERTY(int numberPrecision READ numberPrecision WRITE setNumberPrecision) + Q_PROPERTY(double tickStep READ tickStep WRITE setTickStep) + Q_PROPERTY(QVector tickVector READ tickVector WRITE setTickVector) + Q_PROPERTY(QVector tickVectorLabels READ tickVectorLabels WRITE setTickVectorLabels) + Q_PROPERTY(int tickLengthIn READ tickLengthIn WRITE setTickLengthIn) + Q_PROPERTY(int tickLengthOut READ tickLengthOut WRITE setTickLengthOut) + Q_PROPERTY(int subTickCount READ subTickCount WRITE setSubTickCount) + Q_PROPERTY(int subTickLengthIn READ subTickLengthIn WRITE setSubTickLengthIn) + Q_PROPERTY(int subTickLengthOut READ subTickLengthOut WRITE setSubTickLengthOut) + Q_PROPERTY(QPen basePen READ basePen WRITE setBasePen) + Q_PROPERTY(QPen tickPen READ tickPen WRITE setTickPen) + Q_PROPERTY(QPen subTickPen READ subTickPen WRITE setSubTickPen) + Q_PROPERTY(QFont labelFont READ labelFont WRITE setLabelFont) + Q_PROPERTY(QColor labelColor READ labelColor WRITE setLabelColor) + Q_PROPERTY(QString label READ label WRITE setLabel) + Q_PROPERTY(int labelPadding READ labelPadding WRITE setLabelPadding) + Q_PROPERTY(int padding READ padding WRITE setPadding) + Q_PROPERTY(int offset READ offset WRITE setOffset) + Q_PROPERTY(SelectableParts selectedParts READ selectedParts WRITE setSelectedParts NOTIFY selectionChanged) + Q_PROPERTY(SelectableParts selectableParts READ selectableParts WRITE setSelectableParts NOTIFY selectableChanged) + Q_PROPERTY(QFont selectedTickLabelFont READ selectedTickLabelFont WRITE setSelectedTickLabelFont) + Q_PROPERTY(QFont selectedLabelFont READ selectedLabelFont WRITE setSelectedLabelFont) + Q_PROPERTY(QColor selectedTickLabelColor READ selectedTickLabelColor WRITE setSelectedTickLabelColor) + Q_PROPERTY(QColor selectedLabelColor READ selectedLabelColor WRITE setSelectedLabelColor) + Q_PROPERTY(QPen selectedBasePen READ selectedBasePen WRITE setSelectedBasePen) + Q_PROPERTY(QPen selectedTickPen READ selectedTickPen WRITE setSelectedTickPen) + Q_PROPERTY(QPen selectedSubTickPen READ selectedSubTickPen WRITE setSelectedSubTickPen) + Q_PROPERTY(QCPLineEnding lowerEnding READ lowerEnding WRITE setLowerEnding) + Q_PROPERTY(QCPLineEnding upperEnding READ upperEnding WRITE setUpperEnding) + Q_PROPERTY(QCPGrid* grid READ grid) + /// \endcond +public: + /*! + Defines at which side of the axis rect the axis will appear. This also affects how the tick + marks are drawn, on which side the labels are placed etc. + */ + enum AxisType { atLeft = 0x01 ///< 0x01 Axis is vertical and on the left side of the axis rect + ,atRight = 0x02 ///< 0x02 Axis is vertical and on the right side of the axis rect + ,atTop = 0x04 ///< 0x04 Axis is horizontal and on the top side of the axis rect + ,atBottom = 0x08 ///< 0x08 Axis is horizontal and on the bottom side of the axis rect + }; + Q_FLAGS(AxisType AxisTypes) + Q_DECLARE_FLAGS(AxisTypes, AxisType) + /*! + When automatic tick label generation is enabled (\ref setAutoTickLabels), defines how the + coordinate of the tick is interpreted, i.e. translated into a string. + + \see setTickLabelType + */ + enum LabelType { ltNumber ///< Tick coordinate is regarded as normal number and will be displayed as such. (see \ref setNumberFormat) + ,ltDateTime ///< Tick coordinate is regarded as a date/time (seconds since 1970-01-01T00:00:00 UTC) and will be displayed and formatted as such. (for details, see \ref setDateTimeFormat) + }; + Q_ENUMS(LabelType) + /*! + Defines on which side of the axis the tick labels (numbers) shall appear. + + \see setTickLabelSide + */ + enum LabelSide { lsInside ///< Tick labels will be displayed inside the axis rect and clipped to the inner axis rect + ,lsOutside ///< Tick labels will be displayed outside the axis rect + }; + Q_ENUMS(LabelSide) + /*! + Defines the scale of an axis. + \see setScaleType + */ + enum ScaleType { stLinear ///< Linear scaling + ,stLogarithmic ///< Logarithmic scaling with correspondingly transformed plots and (major) tick marks at every base power (see \ref setScaleLogBase). + }; + Q_ENUMS(ScaleType) + /*! + Defines the selectable parts of an axis. + \see setSelectableParts, setSelectedParts + */ + enum SelectablePart { spNone = 0 ///< None of the selectable parts + ,spAxis = 0x001 ///< The axis backbone and tick marks + ,spTickLabels = 0x002 ///< Tick labels (numbers) of this axis (as a whole, not individually) + ,spAxisLabel = 0x004 ///< The axis label + }; + Q_FLAGS(SelectablePart SelectableParts) + Q_DECLARE_FLAGS(SelectableParts, SelectablePart) + + explicit QCPAxis(QCPAxisRect *parent, AxisType type); + virtual ~QCPAxis(); + + // getters: + AxisType axisType() const { return mAxisType; } + QCPAxisRect *axisRect() const { return mAxisRect; } + ScaleType scaleType() const { return mScaleType; } + double scaleLogBase() const { return mScaleLogBase; } + const QCPRange range() const { return mRange; } + bool rangeReversed() const { return mRangeReversed; } + bool autoTicks() const { return mAutoTicks; } + int autoTickCount() const { return mAutoTickCount; } + bool autoTickLabels() const { return mAutoTickLabels; } + bool autoTickStep() const { return mAutoTickStep; } + bool autoSubTicks() const { return mAutoSubTicks; } + bool ticks() const { return mTicks; } + bool tickLabels() const { return mTickLabels; } + int tickLabelPadding() const; + LabelType tickLabelType() const { return mTickLabelType; } + QFont tickLabelFont() const { return mTickLabelFont; } + QColor tickLabelColor() const { return mTickLabelColor; } + double tickLabelRotation() const; + LabelSide tickLabelSide() const; + QString dateTimeFormat() const { return mDateTimeFormat; } + Qt::TimeSpec dateTimeSpec() const { return mDateTimeSpec; } + QString numberFormat() const; + int numberPrecision() const { return mNumberPrecision; } + double tickStep() const { return mTickStep; } + QVector tickVector() const { return mTickVector; } + QVector tickVectorLabels() const { return mTickVectorLabels; } + int tickLengthIn() const; + int tickLengthOut() const; + int subTickCount() const { return mSubTickCount; } + int subTickLengthIn() const; + int subTickLengthOut() const; + QPen basePen() const { return mBasePen; } + QPen tickPen() const { return mTickPen; } + QPen subTickPen() const { return mSubTickPen; } + QFont labelFont() const { return mLabelFont; } + QColor labelColor() const { return mLabelColor; } + QString label() const { return mLabel; } + int labelPadding() const; + int padding() const { return mPadding; } + int offset() const; + SelectableParts selectedParts() const { return mSelectedParts; } + SelectableParts selectableParts() const { return mSelectableParts; } + QFont selectedTickLabelFont() const { return mSelectedTickLabelFont; } + QFont selectedLabelFont() const { return mSelectedLabelFont; } + QColor selectedTickLabelColor() const { return mSelectedTickLabelColor; } + QColor selectedLabelColor() const { return mSelectedLabelColor; } + QPen selectedBasePen() const { return mSelectedBasePen; } + QPen selectedTickPen() const { return mSelectedTickPen; } + QPen selectedSubTickPen() const { return mSelectedSubTickPen; } + QCPLineEnding lowerEnding() const; + QCPLineEnding upperEnding() const; + QCPGrid *grid() const { return mGrid; } + + // setters: + Q_SLOT void setScaleType(QCPAxis::ScaleType type); + void setScaleLogBase(double base); + Q_SLOT void setRange(const QCPRange &range); + void setRange(double lower, double upper); + void setRange(double position, double size, Qt::AlignmentFlag alignment); + void setRangeLower(double lower); + void setRangeUpper(double upper); + void setRangeReversed(bool reversed); + void setAutoTicks(bool on); + void setAutoTickCount(int approximateCount); + void setAutoTickLabels(bool on); + void setAutoTickStep(bool on); + void setAutoSubTicks(bool on); + void setTicks(bool show); + void setTickLabels(bool show); + void setTickLabelPadding(int padding); + void setTickLabelType(LabelType type); + void setTickLabelFont(const QFont &font); + void setTickLabelColor(const QColor &color); + void setTickLabelRotation(double degrees); + void setTickLabelSide(LabelSide side); + void setDateTimeFormat(const QString &format); + void setDateTimeSpec(const Qt::TimeSpec &timeSpec); + void setNumberFormat(const QString &formatCode); + void setNumberPrecision(int precision); + void setTickStep(double step); + void setTickVector(const QVector &vec); + void setTickVectorLabels(const QVector &vec); + void setTickLength(int inside, int outside=0); + void setTickLengthIn(int inside); + void setTickLengthOut(int outside); + void setSubTickCount(int count); + void setSubTickLength(int inside, int outside=0); + void setSubTickLengthIn(int inside); + void setSubTickLengthOut(int outside); + void setBasePen(const QPen &pen); + void setTickPen(const QPen &pen); + void setSubTickPen(const QPen &pen); + void setLabelFont(const QFont &font); + void setLabelColor(const QColor &color); + void setLabel(const QString &str); + void setLabelPadding(int padding); + void setPadding(int padding); + void setOffset(int offset); + void setSelectedTickLabelFont(const QFont &font); + void setSelectedLabelFont(const QFont &font); + void setSelectedTickLabelColor(const QColor &color); + void setSelectedLabelColor(const QColor &color); + void setSelectedBasePen(const QPen &pen); + void setSelectedTickPen(const QPen &pen); + void setSelectedSubTickPen(const QPen &pen); + Q_SLOT void setSelectableParts(const QCPAxis::SelectableParts &selectableParts); + Q_SLOT void setSelectedParts(const QCPAxis::SelectableParts &selectedParts); + void setLowerEnding(const QCPLineEnding &ending); + void setUpperEnding(const QCPLineEnding &ending); + + // reimplemented virtual methods: + virtual double selectTest(const QPointF &pos, bool onlySelectable, QVariant *details=0) const; + + // non-property methods: + Qt::Orientation orientation() const { return mOrientation; } + void moveRange(double diff); + void scaleRange(double factor, double center); + void setScaleRatio(const QCPAxis *otherAxis, double ratio=1.0); + void rescale(bool onlyVisiblePlottables=false); + double pixelToCoord(double value) const; + double coordToPixel(double value) const; + SelectablePart getPartAt(const QPointF &pos) const; + QList plottables() const; + QList graphs() const; + QList items() const; + + static AxisType marginSideToAxisType(QCP::MarginSide side); + static Qt::Orientation orientation(AxisType type) { return type==atBottom||type==atTop ? Qt::Horizontal : Qt::Vertical; } + static AxisType opposite(AxisType type); + +signals: + void ticksRequest(); + void rangeChanged(const QCPRange &newRange); + void rangeChanged(const QCPRange &newRange, const QCPRange &oldRange); + void scaleTypeChanged(QCPAxis::ScaleType scaleType); + void selectionChanged(const QCPAxis::SelectableParts &parts); + void selectableChanged(const QCPAxis::SelectableParts &parts); + +protected: + // property members: + // axis base: + AxisType mAxisType; + QCPAxisRect *mAxisRect; + //int mOffset; // in QCPAxisPainter + int mPadding; + Qt::Orientation mOrientation; + SelectableParts mSelectableParts, mSelectedParts; + QPen mBasePen, mSelectedBasePen; + //QCPLineEnding mLowerEnding, mUpperEnding; // in QCPAxisPainter + // axis label: + //int mLabelPadding; // in QCPAxisPainter + QString mLabel; + QFont mLabelFont, mSelectedLabelFont; + QColor mLabelColor, mSelectedLabelColor; + // tick labels: + //int mTickLabelPadding; // in QCPAxisPainter + bool mTickLabels, mAutoTickLabels; + //double mTickLabelRotation; // in QCPAxisPainter + LabelType mTickLabelType; + QFont mTickLabelFont, mSelectedTickLabelFont; + QColor mTickLabelColor, mSelectedTickLabelColor; + QString mDateTimeFormat; + Qt::TimeSpec mDateTimeSpec; + int mNumberPrecision; + QLatin1Char mNumberFormatChar; + bool mNumberBeautifulPowers; + //bool mNumberMultiplyCross; // QCPAxisPainter + // ticks and subticks: + bool mTicks; + double mTickStep; + int mSubTickCount, mAutoTickCount; + bool mAutoTicks, mAutoTickStep, mAutoSubTicks; + //int mTickLengthIn, mTickLengthOut, mSubTickLengthIn, mSubTickLengthOut; // QCPAxisPainter + QPen mTickPen, mSelectedTickPen; + QPen mSubTickPen, mSelectedSubTickPen; + // scale and range: + QCPRange mRange; + bool mRangeReversed; + ScaleType mScaleType; + double mScaleLogBase, mScaleLogBaseLogInv; + + // non-property members: + QCPGrid *mGrid; + QCPAxisPainterPrivate *mAxisPainter; + int mLowestVisibleTick, mHighestVisibleTick; + QVector mTickVector; + QVector mTickVectorLabels; + QVector mSubTickVector; + bool mCachedMarginValid; + int mCachedMargin; + + // introduced virtual methods: + virtual void setupTickVectors(); + virtual void generateAutoTicks(); + virtual int calculateAutoSubTickCount(double tickStep) const; + virtual int calculateMargin(); + + // reimplemented virtual methods: + virtual void applyDefaultAntialiasingHint(QCPPainter *painter) const; + virtual void draw(QCPPainter *painter); + virtual QCP::Interaction selectionCategory() const; + // events: + virtual void selectEvent(QMouseEvent *event, bool additive, const QVariant &details, bool *selectionStateChanged); + virtual void deselectEvent(bool *selectionStateChanged); + + // non-virtual methods: + void visibleTickBounds(int &lowIndex, int &highIndex) const; + double baseLog(double value) const; + double basePow(double value) const; + QPen getBasePen() const; + QPen getTickPen() const; + QPen getSubTickPen() const; + QFont getTickLabelFont() const; + QFont getLabelFont() const; + QColor getTickLabelColor() const; + QColor getLabelColor() const; + +private: + Q_DISABLE_COPY(QCPAxis) + + friend class QCustomPlot; + friend class QCPGrid; + friend class QCPAxisRect; +}; +Q_DECLARE_OPERATORS_FOR_FLAGS(QCPAxis::SelectableParts) +Q_DECLARE_OPERATORS_FOR_FLAGS(QCPAxis::AxisTypes) +Q_DECLARE_METATYPE(QCPAxis::SelectablePart) + + +class QCPAxisPainterPrivate +{ +public: + explicit QCPAxisPainterPrivate(QCustomPlot *parentPlot); + virtual ~QCPAxisPainterPrivate(); + + virtual void draw(QCPPainter *painter); + virtual int size() const; + void clearCache(); + + QRect axisSelectionBox() const { return mAxisSelectionBox; } + QRect tickLabelsSelectionBox() const { return mTickLabelsSelectionBox; } + QRect labelSelectionBox() const { return mLabelSelectionBox; } + + // public property members: + QCPAxis::AxisType type; + QPen basePen; + QCPLineEnding lowerEnding, upperEnding; // directly accessed by QCPAxis setters/getters + int labelPadding; // directly accessed by QCPAxis setters/getters + QFont labelFont; + QColor labelColor; + QString label; + int tickLabelPadding; // directly accessed by QCPAxis setters/getters + double tickLabelRotation; // directly accessed by QCPAxis setters/getters + QCPAxis::LabelSide tickLabelSide; // directly accessed by QCPAxis setters/getters + bool substituteExponent; + bool numberMultiplyCross; // directly accessed by QCPAxis setters/getters + int tickLengthIn, tickLengthOut, subTickLengthIn, subTickLengthOut; // directly accessed by QCPAxis setters/getters + QPen tickPen, subTickPen; + QFont tickLabelFont; + QColor tickLabelColor; + QRect axisRect, viewportRect; + double offset; // directly accessed by QCPAxis setters/getters + bool abbreviateDecimalPowers; + bool reversedEndings; + + QVector subTickPositions; + QVector tickPositions; + QVector tickLabels; + +protected: + struct CachedLabel + { + QPointF offset; + QPixmap pixmap; + }; + struct TickLabelData + { + QString basePart, expPart; + QRect baseBounds, expBounds, totalBounds, rotatedTotalBounds; + QFont baseFont, expFont; + }; + QCustomPlot *mParentPlot; + QByteArray mLabelParameterHash; // to determine whether mLabelCache needs to be cleared due to changed parameters + QCache mLabelCache; + QRect mAxisSelectionBox, mTickLabelsSelectionBox, mLabelSelectionBox; + + virtual QByteArray generateLabelParameterHash() const; + + virtual void placeTickLabel(QCPPainter *painter, double position, int distanceToAxis, const QString &text, QSize *tickLabelsSize); + virtual void drawTickLabel(QCPPainter *painter, double x, double y, const TickLabelData &labelData) const; + virtual TickLabelData getTickLabelData(const QFont &font, const QString &text) const; + virtual QPointF getTickLabelDrawOffset(const TickLabelData &labelData) const; + virtual void getMaxTickLabelSize(const QFont &font, const QString &text, QSize *tickLabelsSize) const; +}; + + +class QCP_LIB_DECL QCPAbstractPlottable : public QCPLayerable +{ + Q_OBJECT + /// \cond INCLUDE_QPROPERTIES + Q_PROPERTY(QString name READ name WRITE setName) + Q_PROPERTY(bool antialiasedFill READ antialiasedFill WRITE setAntialiasedFill) + Q_PROPERTY(bool antialiasedScatters READ antialiasedScatters WRITE setAntialiasedScatters) + Q_PROPERTY(bool antialiasedErrorBars READ antialiasedErrorBars WRITE setAntialiasedErrorBars) + Q_PROPERTY(QPen pen READ pen WRITE setPen) + Q_PROPERTY(QPen selectedPen READ selectedPen WRITE setSelectedPen) + Q_PROPERTY(QBrush brush READ brush WRITE setBrush) + Q_PROPERTY(QBrush selectedBrush READ selectedBrush WRITE setSelectedBrush) + Q_PROPERTY(QCPAxis* keyAxis READ keyAxis WRITE setKeyAxis) + Q_PROPERTY(QCPAxis* valueAxis READ valueAxis WRITE setValueAxis) + Q_PROPERTY(bool selectable READ selectable WRITE setSelectable NOTIFY selectableChanged) + Q_PROPERTY(bool selected READ selected WRITE setSelected NOTIFY selectionChanged) + /// \endcond +public: + QCPAbstractPlottable(QCPAxis *keyAxis, QCPAxis *valueAxis); + + // getters: + QString name() const { return mName; } + bool antialiasedFill() const { return mAntialiasedFill; } + bool antialiasedScatters() const { return mAntialiasedScatters; } + bool antialiasedErrorBars() const { return mAntialiasedErrorBars; } + QPen pen() const { return mPen; } + QPen selectedPen() const { return mSelectedPen; } + QBrush brush() const { return mBrush; } + QBrush selectedBrush() const { return mSelectedBrush; } + QCPAxis *keyAxis() const { return mKeyAxis.data(); } + QCPAxis *valueAxis() const { return mValueAxis.data(); } + bool selectable() const { return mSelectable; } + bool selected() const { return mSelected; } + + // setters: + void setName(const QString &name); + void setAntialiasedFill(bool enabled); + void setAntialiasedScatters(bool enabled); + void setAntialiasedErrorBars(bool enabled); + void setPen(const QPen &pen); + void setSelectedPen(const QPen &pen); + void setBrush(const QBrush &brush); + void setSelectedBrush(const QBrush &brush); + void setKeyAxis(QCPAxis *axis); + void setValueAxis(QCPAxis *axis); + Q_SLOT void setSelectable(bool selectable); + Q_SLOT void setSelected(bool selected); + + // introduced virtual methods: + virtual void clearData() = 0; + virtual double selectTest(const QPointF &pos, bool onlySelectable, QVariant *details=0) const = 0; + virtual bool addToLegend(); + virtual bool removeFromLegend() const; + + // non-property methods: + void rescaleAxes(bool onlyEnlarge=false) const; + void rescaleKeyAxis(bool onlyEnlarge=false) const; + void rescaleValueAxis(bool onlyEnlarge=false) const; + +signals: + void selectionChanged(bool selected); + void selectableChanged(bool selectable); + +protected: + /*! + Represents negative and positive sign domain for passing to \ref getKeyRange and \ref getValueRange. + */ + enum SignDomain { sdNegative ///< The negative sign domain, i.e. numbers smaller than zero + ,sdBoth ///< Both sign domains, including zero, i.e. all (rational) numbers + ,sdPositive ///< The positive sign domain, i.e. numbers greater than zero + }; + + // property members: + QString mName; + bool mAntialiasedFill, mAntialiasedScatters, mAntialiasedErrorBars; + QPen mPen, mSelectedPen; + QBrush mBrush, mSelectedBrush; + QPointer mKeyAxis, mValueAxis; + bool mSelectable, mSelected; + + // reimplemented virtual methods: + virtual QRect clipRect() const; + virtual void draw(QCPPainter *painter) = 0; + virtual QCP::Interaction selectionCategory() const; + void applyDefaultAntialiasingHint(QCPPainter *painter) const; + // events: + virtual void selectEvent(QMouseEvent *event, bool additive, const QVariant &details, bool *selectionStateChanged); + virtual void deselectEvent(bool *selectionStateChanged); + + // introduced virtual methods: + virtual void drawLegendIcon(QCPPainter *painter, const QRectF &rect) const = 0; + virtual QCPRange getKeyRange(bool &foundRange, SignDomain inSignDomain=sdBoth) const = 0; + virtual QCPRange getValueRange(bool &foundRange, SignDomain inSignDomain=sdBoth) const = 0; + + // non-virtual methods: + void coordsToPixels(double key, double value, double &x, double &y) const; + const QPointF coordsToPixels(double key, double value) const; + void pixelsToCoords(double x, double y, double &key, double &value) const; + void pixelsToCoords(const QPointF &pixelPos, double &key, double &value) const; + QPen mainPen() const; + QBrush mainBrush() const; + void applyFillAntialiasingHint(QCPPainter *painter) const; + void applyScattersAntialiasingHint(QCPPainter *painter) const; + void applyErrorBarsAntialiasingHint(QCPPainter *painter) const; + double distSqrToLine(const QPointF &start, const QPointF &end, const QPointF &point) const; + +private: + Q_DISABLE_COPY(QCPAbstractPlottable) + + friend class QCustomPlot; + friend class QCPAxis; + friend class QCPPlottableLegendItem; +}; + + +class QCP_LIB_DECL QCPItemAnchor +{ +public: + QCPItemAnchor(QCustomPlot *parentPlot, QCPAbstractItem *parentItem, const QString name, int anchorId=-1); + virtual ~QCPItemAnchor(); + + // getters: + QString name() const { return mName; } + virtual QPointF pixelPoint() const; + +protected: + // property members: + QString mName; + + // non-property members: + QCustomPlot *mParentPlot; + QCPAbstractItem *mParentItem; + int mAnchorId; + QSet mChildrenX, mChildrenY; + + // introduced virtual methods: + virtual QCPItemPosition *toQCPItemPosition() { return 0; } + + // non-virtual methods: + void addChildX(QCPItemPosition* pos); // called from pos when this anchor is set as parent + void removeChildX(QCPItemPosition *pos); // called from pos when its parent anchor is reset or pos deleted + void addChildY(QCPItemPosition* pos); // called from pos when this anchor is set as parent + void removeChildY(QCPItemPosition *pos); // called from pos when its parent anchor is reset or pos deleted + +private: + Q_DISABLE_COPY(QCPItemAnchor) + + friend class QCPItemPosition; +}; + + + +class QCP_LIB_DECL QCPItemPosition : public QCPItemAnchor +{ +public: + /*! + Defines the ways an item position can be specified. Thus it defines what the numbers passed to + \ref setCoords actually mean. + + \see setType + */ + enum PositionType { ptAbsolute ///< Static positioning in pixels, starting from the top left corner of the viewport/widget. + ,ptViewportRatio ///< Static positioning given by a fraction of the viewport size. For example, if you call setCoords(0, 0), the position will be at the top + ///< left corner of the viewport/widget. setCoords(1, 1) will be at the bottom right corner, setCoords(0.5, 0) will be horizontally centered and + ///< vertically at the top of the viewport/widget, etc. + ,ptAxisRectRatio ///< Static positioning given by a fraction of the axis rect size (see \ref setAxisRect). For example, if you call setCoords(0, 0), the position will be at the top + ///< left corner of the axis rect. setCoords(1, 1) will be at the bottom right corner, setCoords(0.5, 0) will be horizontally centered and + ///< vertically at the top of the axis rect, etc. You can also go beyond the axis rect by providing negative coordinates or coordinates larger than 1. + ,ptPlotCoords ///< Dynamic positioning at a plot coordinate defined by two axes (see \ref setAxes). + }; + + QCPItemPosition(QCustomPlot *parentPlot, QCPAbstractItem *parentItem, const QString name); + virtual ~QCPItemPosition(); + + // getters: + PositionType type() const { return typeX(); } + PositionType typeX() const { return mPositionTypeX; } + PositionType typeY() const { return mPositionTypeY; } + QCPItemAnchor *parentAnchor() const { return parentAnchorX(); } + QCPItemAnchor *parentAnchorX() const { return mParentAnchorX; } + QCPItemAnchor *parentAnchorY() const { return mParentAnchorY; } + double key() const { return mKey; } + double value() const { return mValue; } + QPointF coords() const { return QPointF(mKey, mValue); } + QCPAxis *keyAxis() const { return mKeyAxis.data(); } + QCPAxis *valueAxis() const { return mValueAxis.data(); } + QCPAxisRect *axisRect() const; + virtual QPointF pixelPoint() const; + + // setters: + void setType(PositionType type); + void setTypeX(PositionType type); + void setTypeY(PositionType type); + bool setParentAnchor(QCPItemAnchor *parentAnchor, bool keepPixelPosition=false); + bool setParentAnchorX(QCPItemAnchor *parentAnchor, bool keepPixelPosition=false); + bool setParentAnchorY(QCPItemAnchor *parentAnchor, bool keepPixelPosition=false); + void setCoords(double key, double value); + void setCoords(const QPointF &coords); + void setAxes(QCPAxis* keyAxis, QCPAxis* valueAxis); + void setAxisRect(QCPAxisRect *axisRect); + void setPixelPoint(const QPointF &pixelPoint); + +protected: + // property members: + PositionType mPositionTypeX, mPositionTypeY; + QPointer mKeyAxis, mValueAxis; + QPointer mAxisRect; + double mKey, mValue; + QCPItemAnchor *mParentAnchorX, *mParentAnchorY; + + // reimplemented virtual methods: + virtual QCPItemPosition *toQCPItemPosition() { return this; } + +private: + Q_DISABLE_COPY(QCPItemPosition) + +}; + + +class QCP_LIB_DECL QCPAbstractItem : public QCPLayerable +{ + Q_OBJECT + /// \cond INCLUDE_QPROPERTIES + Q_PROPERTY(bool clipToAxisRect READ clipToAxisRect WRITE setClipToAxisRect) + Q_PROPERTY(QCPAxisRect* clipAxisRect READ clipAxisRect WRITE setClipAxisRect) + Q_PROPERTY(bool selectable READ selectable WRITE setSelectable NOTIFY selectableChanged) + Q_PROPERTY(bool selected READ selected WRITE setSelected NOTIFY selectionChanged) + /// \endcond +public: + QCPAbstractItem(QCustomPlot *parentPlot); + virtual ~QCPAbstractItem(); + + // getters: + bool clipToAxisRect() const { return mClipToAxisRect; } + QCPAxisRect *clipAxisRect() const; + bool selectable() const { return mSelectable; } + bool selected() const { return mSelected; } + + // setters: + void setClipToAxisRect(bool clip); + void setClipAxisRect(QCPAxisRect *rect); + Q_SLOT void setSelectable(bool selectable); + Q_SLOT void setSelected(bool selected); + + // reimplemented virtual methods: + virtual double selectTest(const QPointF &pos, bool onlySelectable, QVariant *details=0) const = 0; + + // non-virtual methods: + QList positions() const { return mPositions; } + QList anchors() const { return mAnchors; } + QCPItemPosition *position(const QString &name) const; + QCPItemAnchor *anchor(const QString &name) const; + bool hasAnchor(const QString &name) const; + +signals: + void selectionChanged(bool selected); + void selectableChanged(bool selectable); + +protected: + // property members: + bool mClipToAxisRect; + QPointer mClipAxisRect; + QList mPositions; + QList mAnchors; + bool mSelectable, mSelected; + + // reimplemented virtual methods: + virtual QCP::Interaction selectionCategory() const; + virtual QRect clipRect() const; + virtual void applyDefaultAntialiasingHint(QCPPainter *painter) const; + virtual void draw(QCPPainter *painter) = 0; + // events: + virtual void selectEvent(QMouseEvent *event, bool additive, const QVariant &details, bool *selectionStateChanged); + virtual void deselectEvent(bool *selectionStateChanged); + + // introduced virtual methods: + virtual QPointF anchorPixelPoint(int anchorId) const; + + // non-virtual methods: + double distSqrToLine(const QPointF &start, const QPointF &end, const QPointF &point) const; + double rectSelectTest(const QRectF &rect, const QPointF &pos, bool filledRect) const; + QCPItemPosition *createPosition(const QString &name); + QCPItemAnchor *createAnchor(const QString &name, int anchorId); + +private: + Q_DISABLE_COPY(QCPAbstractItem) + + friend class QCustomPlot; + friend class QCPItemAnchor; +}; + + +class QCP_LIB_DECL QCustomPlot : public QWidget +{ + Q_OBJECT + /// \cond INCLUDE_QPROPERTIES + Q_PROPERTY(QRect viewport READ viewport WRITE setViewport) + Q_PROPERTY(QPixmap background READ background WRITE setBackground) + Q_PROPERTY(bool backgroundScaled READ backgroundScaled WRITE setBackgroundScaled) + Q_PROPERTY(Qt::AspectRatioMode backgroundScaledMode READ backgroundScaledMode WRITE setBackgroundScaledMode) + Q_PROPERTY(QCPLayoutGrid* plotLayout READ plotLayout) + Q_PROPERTY(bool autoAddPlottableToLegend READ autoAddPlottableToLegend WRITE setAutoAddPlottableToLegend) + Q_PROPERTY(int selectionTolerance READ selectionTolerance WRITE setSelectionTolerance) + Q_PROPERTY(bool noAntialiasingOnDrag READ noAntialiasingOnDrag WRITE setNoAntialiasingOnDrag) + Q_PROPERTY(Qt::KeyboardModifier multiSelectModifier READ multiSelectModifier WRITE setMultiSelectModifier) + /// \endcond +public: + /*! + Defines how a layer should be inserted relative to an other layer. + + \see addLayer, moveLayer + */ + enum LayerInsertMode { limBelow ///< Layer is inserted below other layer + ,limAbove ///< Layer is inserted above other layer + }; + Q_ENUMS(LayerInsertMode) + + /*! + Defines with what timing the QCustomPlot surface is refreshed after a replot. + + \see replot + */ + enum RefreshPriority { rpImmediate ///< The QCustomPlot surface is immediately refreshed, by calling QWidget::repaint() after the replot + ,rpQueued ///< Queues the refresh such that it is performed at a slightly delayed point in time after the replot, by calling QWidget::update() after the replot + ,rpHint ///< Whether to use immediate repaint or queued update depends on whether the plotting hint \ref QCP::phForceRepaint is set, see \ref setPlottingHints. + }; + + explicit QCustomPlot(QWidget *parent = 0); + virtual ~QCustomPlot(); + + // getters: + QRect viewport() const { return mViewport; } + QPixmap background() const { return mBackgroundPixmap; } + bool backgroundScaled() const { return mBackgroundScaled; } + Qt::AspectRatioMode backgroundScaledMode() const { return mBackgroundScaledMode; } + QCPLayoutGrid *plotLayout() const { return mPlotLayout; } + QCP::AntialiasedElements antialiasedElements() const { return mAntialiasedElements; } + QCP::AntialiasedElements notAntialiasedElements() const { return mNotAntialiasedElements; } + bool autoAddPlottableToLegend() const { return mAutoAddPlottableToLegend; } + const QCP::Interactions interactions() const { return mInteractions; } + int selectionTolerance() const { return mSelectionTolerance; } + bool noAntialiasingOnDrag() const { return mNoAntialiasingOnDrag; } + QCP::PlottingHints plottingHints() const { return mPlottingHints; } + Qt::KeyboardModifier multiSelectModifier() const { return mMultiSelectModifier; } + + // setters: + void setViewport(const QRect &rect); + void setBackground(const QPixmap &pm); + void setBackground(const QPixmap &pm, bool scaled, Qt::AspectRatioMode mode=Qt::KeepAspectRatioByExpanding); + void setBackground(const QBrush &brush); + void setBackgroundScaled(bool scaled); + void setBackgroundScaledMode(Qt::AspectRatioMode mode); + void setAntialiasedElements(const QCP::AntialiasedElements &antialiasedElements); + void setAntialiasedElement(QCP::AntialiasedElement antialiasedElement, bool enabled=true); + void setNotAntialiasedElements(const QCP::AntialiasedElements ¬AntialiasedElements); + void setNotAntialiasedElement(QCP::AntialiasedElement notAntialiasedElement, bool enabled=true); + void setAutoAddPlottableToLegend(bool on); + void setInteractions(const QCP::Interactions &interactions); + void setInteraction(const QCP::Interaction &interaction, bool enabled=true); + void setSelectionTolerance(int pixels); + void setNoAntialiasingOnDrag(bool enabled); + void setPlottingHints(const QCP::PlottingHints &hints); + void setPlottingHint(QCP::PlottingHint hint, bool enabled=true); + void setMultiSelectModifier(Qt::KeyboardModifier modifier); + + // non-property methods: + // plottable interface: + QCPAbstractPlottable *plottable(int index); + QCPAbstractPlottable *plottable(); + bool addPlottable(QCPAbstractPlottable *plottable); + bool removePlottable(QCPAbstractPlottable *plottable); + bool removePlottable(int index); + int clearPlottables(); + int plottableCount() const; + QList selectedPlottables() const; + QCPAbstractPlottable *plottableAt(const QPointF &pos, bool onlySelectable=false) const; + bool hasPlottable(QCPAbstractPlottable *plottable) const; + + // specialized interface for QCPGraph: + QCPGraph *graph(int index) const; + QCPGraph *graph() const; + QCPGraph *addGraph(QCPAxis *keyAxis=0, QCPAxis *valueAxis=0); + bool removeGraph(QCPGraph *graph); + bool removeGraph(int index); + int clearGraphs(); + int graphCount() const; + QList selectedGraphs() const; + + // item interface: + QCPAbstractItem *item(int index) const; + QCPAbstractItem *item() const; + bool addItem(QCPAbstractItem* item); + bool removeItem(QCPAbstractItem *item); + bool removeItem(int index); + int clearItems(); + int itemCount() const; + QList selectedItems() const; + QCPAbstractItem *itemAt(const QPointF &pos, bool onlySelectable=false) const; + bool hasItem(QCPAbstractItem *item) const; + + // layer interface: + QCPLayer *layer(const QString &name) const; + QCPLayer *layer(int index) const; + QCPLayer *currentLayer() const; + bool setCurrentLayer(const QString &name); + bool setCurrentLayer(QCPLayer *layer); + int layerCount() const; + bool addLayer(const QString &name, QCPLayer *otherLayer=0, LayerInsertMode insertMode=limAbove); + bool removeLayer(QCPLayer *layer); + bool moveLayer(QCPLayer *layer, QCPLayer *otherLayer, LayerInsertMode insertMode=limAbove); + + // axis rect/layout interface: + int axisRectCount() const; + QCPAxisRect* axisRect(int index=0) const; + QList axisRects() const; + QCPLayoutElement* layoutElementAt(const QPointF &pos) const; + Q_SLOT void rescaleAxes(bool onlyVisiblePlottables=false); + + QList selectedAxes() const; + QList selectedLegends() const; + Q_SLOT void deselectAll(); + + bool savePdf(const QString &fileName, bool noCosmeticPen=false, int width=0, int height=0, const QString &pdfCreator=QString(), const QString &pdfTitle=QString()); + bool savePng(const QString &fileName, int width=0, int height=0, double scale=1.0, int quality=-1); + bool saveJpg(const QString &fileName, int width=0, int height=0, double scale=1.0, int quality=-1); + bool saveBmp(const QString &fileName, int width=0, int height=0, double scale=1.0); + bool saveRastered(const QString &fileName, int width, int height, double scale, const char *format, int quality=-1); + QPixmap toPixmap(int width=0, int height=0, double scale=1.0); + void toPainter(QCPPainter *painter, int width=0, int height=0); + Q_SLOT void replot(QCustomPlot::RefreshPriority refreshPriority=QCustomPlot::rpHint); + + QCPAxis *xAxis, *yAxis, *xAxis2, *yAxis2; + QCPLegend *legend; + +signals: + void mouseDoubleClick(QMouseEvent *event); + void mousePress(QMouseEvent *event); + void mouseMove(QMouseEvent *event); + void mouseRelease(QMouseEvent *event); + void mouseWheel(QWheelEvent *event); + + void plottableClick(QCPAbstractPlottable *plottable, QMouseEvent *event); + void plottableDoubleClick(QCPAbstractPlottable *plottable, QMouseEvent *event); + void itemClick(QCPAbstractItem *item, QMouseEvent *event); + void itemDoubleClick(QCPAbstractItem *item, QMouseEvent *event); + void axisClick(QCPAxis *axis, QCPAxis::SelectablePart part, QMouseEvent *event); + void axisDoubleClick(QCPAxis *axis, QCPAxis::SelectablePart part, QMouseEvent *event); + void legendClick(QCPLegend *legend, QCPAbstractLegendItem *item, QMouseEvent *event); + void legendDoubleClick(QCPLegend *legend, QCPAbstractLegendItem *item, QMouseEvent *event); + void titleClick(QMouseEvent *event, QCPPlotTitle *title); + void titleDoubleClick(QMouseEvent *event, QCPPlotTitle *title); + + void selectionChangedByUser(); + void beforeReplot(); + void afterReplot(); + +protected: + // property members: + QRect mViewport; + QCPLayoutGrid *mPlotLayout; + bool mAutoAddPlottableToLegend; + QList mPlottables; + QList mGraphs; // extra list of plottables also in mPlottables that are of type QCPGraph + QList mItems; + QList mLayers; + QCP::AntialiasedElements mAntialiasedElements, mNotAntialiasedElements; + QCP::Interactions mInteractions; + int mSelectionTolerance; + bool mNoAntialiasingOnDrag; + QBrush mBackgroundBrush; + QPixmap mBackgroundPixmap; + QPixmap mScaledBackgroundPixmap; + bool mBackgroundScaled; + Qt::AspectRatioMode mBackgroundScaledMode; + QCPLayer *mCurrentLayer; + QCP::PlottingHints mPlottingHints; + Qt::KeyboardModifier mMultiSelectModifier; + + // non-property members: + QPixmap mPaintBuffer; + QPoint mMousePressPos; + QPointer mMouseEventElement; + bool mReplotting; + + // reimplemented virtual methods: + virtual QSize minimumSizeHint() const; + virtual QSize sizeHint() const; + virtual void paintEvent(QPaintEvent *event); + virtual void resizeEvent(QResizeEvent *event); + virtual void mouseDoubleClickEvent(QMouseEvent *event); + virtual void mousePressEvent(QMouseEvent *event); + virtual void mouseMoveEvent(QMouseEvent *event); + virtual void mouseReleaseEvent(QMouseEvent *event); + virtual void wheelEvent(QWheelEvent *event); + + // introduced virtual methods: + virtual void draw(QCPPainter *painter); + virtual void axisRemoved(QCPAxis *axis); + virtual void legendRemoved(QCPLegend *legend); + + // non-virtual methods: + void updateLayerIndices() const; + QCPLayerable *layerableAt(const QPointF &pos, bool onlySelectable, QVariant *selectionDetails=0) const; + void drawBackground(QCPPainter *painter); + + friend class QCPLegend; + friend class QCPAxis; + friend class QCPLayer; + friend class QCPAxisRect; +}; + + +class QCP_LIB_DECL QCPColorGradient +{ + Q_GADGET +public: + /*! + Defines the color spaces in which color interpolation between gradient stops can be performed. + + \see setColorInterpolation + */ + enum ColorInterpolation { ciRGB ///< Color channels red, green and blue are linearly interpolated + ,ciHSV ///< Color channels hue, saturation and value are linearly interpolated (The hue is interpolated over the shortest angle distance) + }; + Q_ENUMS(ColorInterpolation) + + /*! + Defines the available presets that can be loaded with \ref loadPreset. See the documentation + there for an image of the presets. + */ + enum GradientPreset { gpGrayscale ///< Continuous lightness from black to white (suited for non-biased data representation) + ,gpHot ///< Continuous lightness from black over firey colors to white (suited for non-biased data representation) + ,gpCold ///< Continuous lightness from black over icey colors to white (suited for non-biased data representation) + ,gpNight ///< Continuous lightness from black over weak blueish colors to white (suited for non-biased data representation) + ,gpCandy ///< Blue over pink to white + ,gpGeography ///< Colors suitable to represent different elevations on geographical maps + ,gpIon ///< Half hue spectrum from black over purple to blue and finally green (creates banding illusion but allows more precise magnitude estimates) + ,gpThermal ///< Colors suitable for thermal imaging, ranging from dark blue over purple to orange, yellow and white + ,gpPolar ///< Colors suitable to emphasize polarity around the center, with blue for negative, black in the middle and red for positive values + ,gpSpectrum ///< An approximation of the visible light spectrum (creates banding illusion but allows more precise magnitude estimates) + ,gpJet ///< Hue variation similar to a spectrum, often used in numerical visualization (creates banding illusion but allows more precise magnitude estimates) + ,gpHues ///< Full hue cycle, with highest and lowest color red (suitable for periodic data, such as angles and phases, see \ref setPeriodic) + }; + Q_ENUMS(GradientPreset) + + QCPColorGradient(GradientPreset preset=gpCold); + bool operator==(const QCPColorGradient &other) const; + bool operator!=(const QCPColorGradient &other) const { return !(*this == other); } + + // getters: + int levelCount() const { return mLevelCount; } + QMap colorStops() const { return mColorStops; } + ColorInterpolation colorInterpolation() const { return mColorInterpolation; } + bool periodic() const { return mPeriodic; } + + // setters: + void setLevelCount(int n); + void setColorStops(const QMap &colorStops); + void setColorStopAt(double position, const QColor &color); + void setColorInterpolation(ColorInterpolation interpolation); + void setPeriodic(bool enabled); + + // non-property methods: + void colorize(const double *data, const QCPRange &range, QRgb *scanLine, int n, int dataIndexFactor=1, bool logarithmic=false); + QRgb color(double position, const QCPRange &range, bool logarithmic=false); + void loadPreset(GradientPreset preset); + void clearColorStops(); + QCPColorGradient inverted() const; + +protected: + void updateColorBuffer(); + + // property members: + int mLevelCount; + QMap mColorStops; + ColorInterpolation mColorInterpolation; + bool mPeriodic; + + // non-property members: + QVector mColorBuffer; + bool mColorBufferInvalidated; +}; + + +class QCP_LIB_DECL QCPAxisRect : public QCPLayoutElement +{ + Q_OBJECT + /// \cond INCLUDE_QPROPERTIES + Q_PROPERTY(QPixmap background READ background WRITE setBackground) + Q_PROPERTY(bool backgroundScaled READ backgroundScaled WRITE setBackgroundScaled) + Q_PROPERTY(Qt::AspectRatioMode backgroundScaledMode READ backgroundScaledMode WRITE setBackgroundScaledMode) + Q_PROPERTY(Qt::Orientations rangeDrag READ rangeDrag WRITE setRangeDrag) + Q_PROPERTY(Qt::Orientations rangeZoom READ rangeZoom WRITE setRangeZoom) + /// \endcond +public: + explicit QCPAxisRect(QCustomPlot *parentPlot, bool setupDefaultAxes=true); + virtual ~QCPAxisRect(); + + // getters: + QPixmap background() const { return mBackgroundPixmap; } + bool backgroundScaled() const { return mBackgroundScaled; } + Qt::AspectRatioMode backgroundScaledMode() const { return mBackgroundScaledMode; } + Qt::Orientations rangeDrag() const { return mRangeDrag; } + Qt::Orientations rangeZoom() const { return mRangeZoom; } + QCPAxis *rangeDragAxis(Qt::Orientation orientation); + QCPAxis *rangeZoomAxis(Qt::Orientation orientation); + double rangeZoomFactor(Qt::Orientation orientation); + + // setters: + void setBackground(const QPixmap &pm); + void setBackground(const QPixmap &pm, bool scaled, Qt::AspectRatioMode mode=Qt::KeepAspectRatioByExpanding); + void setBackground(const QBrush &brush); + void setBackgroundScaled(bool scaled); + void setBackgroundScaledMode(Qt::AspectRatioMode mode); + void setRangeDrag(Qt::Orientations orientations); + void setRangeZoom(Qt::Orientations orientations); + void setRangeDragAxes(QCPAxis *horizontal, QCPAxis *vertical); + void setRangeZoomAxes(QCPAxis *horizontal, QCPAxis *vertical); + void setRangeZoomFactor(double horizontalFactor, double verticalFactor); + void setRangeZoomFactor(double factor); + + // non-property methods: + int axisCount(QCPAxis::AxisType type) const; + QCPAxis *axis(QCPAxis::AxisType type, int index=0) const; + QList axes(QCPAxis::AxisTypes types) const; + QList axes() const; + QCPAxis *addAxis(QCPAxis::AxisType type, QCPAxis *axis=0); + QList addAxes(QCPAxis::AxisTypes types); + bool removeAxis(QCPAxis *axis); + QCPLayoutInset *insetLayout() const { return mInsetLayout; } + + void setupFullAxesBox(bool connectRanges=false); + QList plottables() const; + QList graphs() const; + QList items() const; + + // read-only interface imitating a QRect: + int left() const { return mRect.left(); } + int right() const { return mRect.right(); } + int top() const { return mRect.top(); } + int bottom() const { return mRect.bottom(); } + int width() const { return mRect.width(); } + int height() const { return mRect.height(); } + QSize size() const { return mRect.size(); } + QPoint topLeft() const { return mRect.topLeft(); } + QPoint topRight() const { return mRect.topRight(); } + QPoint bottomLeft() const { return mRect.bottomLeft(); } + QPoint bottomRight() const { return mRect.bottomRight(); } + QPoint center() const { return mRect.center(); } + + // reimplemented virtual methods: + virtual void update(UpdatePhase phase); + virtual QList elements(bool recursive) const; + +protected: + // property members: + QBrush mBackgroundBrush; + QPixmap mBackgroundPixmap; + QPixmap mScaledBackgroundPixmap; + bool mBackgroundScaled; + Qt::AspectRatioMode mBackgroundScaledMode; + QCPLayoutInset *mInsetLayout; + Qt::Orientations mRangeDrag, mRangeZoom; + QPointer mRangeDragHorzAxis, mRangeDragVertAxis, mRangeZoomHorzAxis, mRangeZoomVertAxis; + double mRangeZoomFactorHorz, mRangeZoomFactorVert; + // non-property members: + QCPRange mDragStartHorzRange, mDragStartVertRange; + QCP::AntialiasedElements mAADragBackup, mNotAADragBackup; + QPoint mDragStart; + bool mDragging; + QHash > mAxes; + + // reimplemented virtual methods: + virtual void applyDefaultAntialiasingHint(QCPPainter *painter) const; + virtual void draw(QCPPainter *painter); + virtual int calculateAutoMargin(QCP::MarginSide side); + // events: + virtual void mousePressEvent(QMouseEvent *event); + virtual void mouseMoveEvent(QMouseEvent *event); + virtual void mouseReleaseEvent(QMouseEvent *event); + virtual void wheelEvent(QWheelEvent *event); + + // non-property methods: + void drawBackground(QCPPainter *painter); + void updateAxesOffset(QCPAxis::AxisType type); + +private: + Q_DISABLE_COPY(QCPAxisRect) + + friend class QCustomPlot; +}; + + +class QCP_LIB_DECL QCPAbstractLegendItem : public QCPLayoutElement +{ + Q_OBJECT + /// \cond INCLUDE_QPROPERTIES + Q_PROPERTY(QCPLegend* parentLegend READ parentLegend) + Q_PROPERTY(QFont font READ font WRITE setFont) + Q_PROPERTY(QColor textColor READ textColor WRITE setTextColor) + Q_PROPERTY(QFont selectedFont READ selectedFont WRITE setSelectedFont) + Q_PROPERTY(QColor selectedTextColor READ selectedTextColor WRITE setSelectedTextColor) + Q_PROPERTY(bool selectable READ selectable WRITE setSelectable NOTIFY selectionChanged) + Q_PROPERTY(bool selected READ selected WRITE setSelected NOTIFY selectableChanged) + /// \endcond +public: + explicit QCPAbstractLegendItem(QCPLegend *parent); + + // getters: + QCPLegend *parentLegend() const { return mParentLegend; } + QFont font() const { return mFont; } + QColor textColor() const { return mTextColor; } + QFont selectedFont() const { return mSelectedFont; } + QColor selectedTextColor() const { return mSelectedTextColor; } + bool selectable() const { return mSelectable; } + bool selected() const { return mSelected; } + + // setters: + void setFont(const QFont &font); + void setTextColor(const QColor &color); + void setSelectedFont(const QFont &font); + void setSelectedTextColor(const QColor &color); + Q_SLOT void setSelectable(bool selectable); + Q_SLOT void setSelected(bool selected); + + // reimplemented virtual methods: + virtual double selectTest(const QPointF &pos, bool onlySelectable, QVariant *details=0) const; + +signals: + void selectionChanged(bool selected); + void selectableChanged(bool selectable); + +protected: + // property members: + QCPLegend *mParentLegend; + QFont mFont; + QColor mTextColor; + QFont mSelectedFont; + QColor mSelectedTextColor; + bool mSelectable, mSelected; + + // reimplemented virtual methods: + virtual QCP::Interaction selectionCategory() const; + virtual void applyDefaultAntialiasingHint(QCPPainter *painter) const; + virtual QRect clipRect() const; + virtual void draw(QCPPainter *painter) = 0; + // events: + virtual void selectEvent(QMouseEvent *event, bool additive, const QVariant &details, bool *selectionStateChanged); + virtual void deselectEvent(bool *selectionStateChanged); + +private: + Q_DISABLE_COPY(QCPAbstractLegendItem) + + friend class QCPLegend; +}; + + +class QCP_LIB_DECL QCPPlottableLegendItem : public QCPAbstractLegendItem +{ + Q_OBJECT +public: + QCPPlottableLegendItem(QCPLegend *parent, QCPAbstractPlottable *plottable); + + // getters: + QCPAbstractPlottable *plottable() { return mPlottable; } + +protected: + // property members: + QCPAbstractPlottable *mPlottable; + + // reimplemented virtual methods: + virtual void draw(QCPPainter *painter); + virtual QSize minimumSizeHint() const; + + // non-virtual methods: + QPen getIconBorderPen() const; + QColor getTextColor() const; + QFont getFont() const; +}; + + +class QCP_LIB_DECL QCPLegend : public QCPLayoutGrid +{ + Q_OBJECT + /// \cond INCLUDE_QPROPERTIES + Q_PROPERTY(QPen borderPen READ borderPen WRITE setBorderPen) + Q_PROPERTY(QBrush brush READ brush WRITE setBrush) + Q_PROPERTY(QFont font READ font WRITE setFont) + Q_PROPERTY(QColor textColor READ textColor WRITE setTextColor) + Q_PROPERTY(QSize iconSize READ iconSize WRITE setIconSize) + Q_PROPERTY(int iconTextPadding READ iconTextPadding WRITE setIconTextPadding) + Q_PROPERTY(QPen iconBorderPen READ iconBorderPen WRITE setIconBorderPen) + Q_PROPERTY(SelectableParts selectableParts READ selectableParts WRITE setSelectableParts NOTIFY selectionChanged) + Q_PROPERTY(SelectableParts selectedParts READ selectedParts WRITE setSelectedParts NOTIFY selectableChanged) + Q_PROPERTY(QPen selectedBorderPen READ selectedBorderPen WRITE setSelectedBorderPen) + Q_PROPERTY(QPen selectedIconBorderPen READ selectedIconBorderPen WRITE setSelectedIconBorderPen) + Q_PROPERTY(QBrush selectedBrush READ selectedBrush WRITE setSelectedBrush) + Q_PROPERTY(QFont selectedFont READ selectedFont WRITE setSelectedFont) + Q_PROPERTY(QColor selectedTextColor READ selectedTextColor WRITE setSelectedTextColor) + /// \endcond +public: + /*! + Defines the selectable parts of a legend + + \see setSelectedParts, setSelectableParts + */ + enum SelectablePart { spNone = 0x000 ///< 0x000 None + ,spLegendBox = 0x001 ///< 0x001 The legend box (frame) + ,spItems = 0x002 ///< 0x002 Legend items individually (see \ref selectedItems) + }; + Q_FLAGS(SelectablePart SelectableParts) + Q_DECLARE_FLAGS(SelectableParts, SelectablePart) + + explicit QCPLegend(); + virtual ~QCPLegend(); + + // getters: + QPen borderPen() const { return mBorderPen; } + QBrush brush() const { return mBrush; } + QFont font() const { return mFont; } + QColor textColor() const { return mTextColor; } + QSize iconSize() const { return mIconSize; } + int iconTextPadding() const { return mIconTextPadding; } + QPen iconBorderPen() const { return mIconBorderPen; } + SelectableParts selectableParts() const { return mSelectableParts; } + SelectableParts selectedParts() const; + QPen selectedBorderPen() const { return mSelectedBorderPen; } + QPen selectedIconBorderPen() const { return mSelectedIconBorderPen; } + QBrush selectedBrush() const { return mSelectedBrush; } + QFont selectedFont() const { return mSelectedFont; } + QColor selectedTextColor() const { return mSelectedTextColor; } + + // setters: + void setBorderPen(const QPen &pen); + void setBrush(const QBrush &brush); + void setFont(const QFont &font); + void setTextColor(const QColor &color); + void setIconSize(const QSize &size); + void setIconSize(int width, int height); + void setIconTextPadding(int padding); + void setIconBorderPen(const QPen &pen); + Q_SLOT void setSelectableParts(const SelectableParts &selectableParts); + Q_SLOT void setSelectedParts(const SelectableParts &selectedParts); + void setSelectedBorderPen(const QPen &pen); + void setSelectedIconBorderPen(const QPen &pen); + void setSelectedBrush(const QBrush &brush); + void setSelectedFont(const QFont &font); + void setSelectedTextColor(const QColor &color); + + // reimplemented virtual methods: + virtual double selectTest(const QPointF &pos, bool onlySelectable, QVariant *details=0) const; + + // non-virtual methods: + QCPAbstractLegendItem *item(int index) const; + QCPPlottableLegendItem *itemWithPlottable(const QCPAbstractPlottable *plottable) const; + int itemCount() const; + bool hasItem(QCPAbstractLegendItem *item) const; + bool hasItemWithPlottable(const QCPAbstractPlottable *plottable) const; + bool addItem(QCPAbstractLegendItem *item); + bool removeItem(int index); + bool removeItem(QCPAbstractLegendItem *item); + void clearItems(); + QList selectedItems() const; + +signals: + void selectionChanged(QCPLegend::SelectableParts parts); + void selectableChanged(QCPLegend::SelectableParts parts); + +protected: + // property members: + QPen mBorderPen, mIconBorderPen; + QBrush mBrush; + QFont mFont; + QColor mTextColor; + QSize mIconSize; + int mIconTextPadding; + SelectableParts mSelectedParts, mSelectableParts; + QPen mSelectedBorderPen, mSelectedIconBorderPen; + QBrush mSelectedBrush; + QFont mSelectedFont; + QColor mSelectedTextColor; + + // reimplemented virtual methods: + virtual void parentPlotInitialized(QCustomPlot *parentPlot); + virtual QCP::Interaction selectionCategory() const; + virtual void applyDefaultAntialiasingHint(QCPPainter *painter) const; + virtual void draw(QCPPainter *painter); + // events: + virtual void selectEvent(QMouseEvent *event, bool additive, const QVariant &details, bool *selectionStateChanged); + virtual void deselectEvent(bool *selectionStateChanged); + + // non-virtual methods: + QPen getBorderPen() const; + QBrush getBrush() const; + +private: + Q_DISABLE_COPY(QCPLegend) + + friend class QCustomPlot; + friend class QCPAbstractLegendItem; +}; +Q_DECLARE_OPERATORS_FOR_FLAGS(QCPLegend::SelectableParts) +Q_DECLARE_METATYPE(QCPLegend::SelectablePart) + + +class QCP_LIB_DECL QCPPlotTitle : public QCPLayoutElement +{ + Q_OBJECT + /// \cond INCLUDE_QPROPERTIES + Q_PROPERTY(QString text READ text WRITE setText) + Q_PROPERTY(QFont font READ font WRITE setFont) + Q_PROPERTY(QColor textColor READ textColor WRITE setTextColor) + Q_PROPERTY(QFont selectedFont READ selectedFont WRITE setSelectedFont) + Q_PROPERTY(QColor selectedTextColor READ selectedTextColor WRITE setSelectedTextColor) + Q_PROPERTY(bool selectable READ selectable WRITE setSelectable NOTIFY selectableChanged) + Q_PROPERTY(bool selected READ selected WRITE setSelected NOTIFY selectionChanged) + /// \endcond +public: + explicit QCPPlotTitle(QCustomPlot *parentPlot); + explicit QCPPlotTitle(QCustomPlot *parentPlot, const QString &text); + + // getters: + QString text() const { return mText; } + QFont font() const { return mFont; } + QColor textColor() const { return mTextColor; } + QFont selectedFont() const { return mSelectedFont; } + QColor selectedTextColor() const { return mSelectedTextColor; } + bool selectable() const { return mSelectable; } + bool selected() const { return mSelected; } + + // setters: + void setText(const QString &text); + void setFont(const QFont &font); + void setTextColor(const QColor &color); + void setSelectedFont(const QFont &font); + void setSelectedTextColor(const QColor &color); + Q_SLOT void setSelectable(bool selectable); + Q_SLOT void setSelected(bool selected); + + // reimplemented virtual methods: + virtual double selectTest(const QPointF &pos, bool onlySelectable, QVariant *details=0) const; + +signals: + void selectionChanged(bool selected); + void selectableChanged(bool selectable); + +protected: + // property members: + QString mText; + QFont mFont; + QColor mTextColor; + QFont mSelectedFont; + QColor mSelectedTextColor; + QRect mTextBoundingRect; + bool mSelectable, mSelected; + + // reimplemented virtual methods: + virtual void applyDefaultAntialiasingHint(QCPPainter *painter) const; + virtual void draw(QCPPainter *painter); + virtual QSize minimumSizeHint() const; + virtual QSize maximumSizeHint() const; + // events: + virtual void selectEvent(QMouseEvent *event, bool additive, const QVariant &details, bool *selectionStateChanged); + virtual void deselectEvent(bool *selectionStateChanged); + + // non-virtual methods: + QFont mainFont() const; + QColor mainTextColor() const; + +private: + Q_DISABLE_COPY(QCPPlotTitle) +}; + + +class QCPColorScaleAxisRectPrivate : public QCPAxisRect +{ + Q_OBJECT +public: + explicit QCPColorScaleAxisRectPrivate(QCPColorScale *parentColorScale); +protected: + QCPColorScale *mParentColorScale; + QImage mGradientImage; + bool mGradientImageInvalidated; + // re-using some methods of QCPAxisRect to make them available to friend class QCPColorScale + using QCPAxisRect::calculateAutoMargin; + using QCPAxisRect::mousePressEvent; + using QCPAxisRect::mouseMoveEvent; + using QCPAxisRect::mouseReleaseEvent; + using QCPAxisRect::wheelEvent; + using QCPAxisRect::update; + virtual void draw(QCPPainter *painter); + void updateGradientImage(); + Q_SLOT void axisSelectionChanged(QCPAxis::SelectableParts selectedParts); + Q_SLOT void axisSelectableChanged(QCPAxis::SelectableParts selectableParts); + friend class QCPColorScale; +}; + + +class QCP_LIB_DECL QCPColorScale : public QCPLayoutElement +{ + Q_OBJECT + /// \cond INCLUDE_QPROPERTIES + Q_PROPERTY(QCPAxis::AxisType type READ type WRITE setType) + Q_PROPERTY(QCPRange dataRange READ dataRange WRITE setDataRange NOTIFY dataRangeChanged) + Q_PROPERTY(QCPAxis::ScaleType dataScaleType READ dataScaleType WRITE setDataScaleType NOTIFY dataScaleTypeChanged) + Q_PROPERTY(QCPColorGradient gradient READ gradient WRITE setGradient NOTIFY gradientChanged) + Q_PROPERTY(QString label READ label WRITE setLabel) + Q_PROPERTY(int barWidth READ barWidth WRITE setBarWidth) + Q_PROPERTY(bool rangeDrag READ rangeDrag WRITE setRangeDrag) + Q_PROPERTY(bool rangeZoom READ rangeZoom WRITE setRangeZoom) + /// \endcond +public: + explicit QCPColorScale(QCustomPlot *parentPlot); + virtual ~QCPColorScale(); + + // getters: + QCPAxis *axis() const { return mColorAxis.data(); } + QCPAxis::AxisType type() const { return mType; } + QCPRange dataRange() const { return mDataRange; } + QCPAxis::ScaleType dataScaleType() const { return mDataScaleType; } + QCPColorGradient gradient() const { return mGradient; } + QString label() const; + int barWidth () const { return mBarWidth; } + bool rangeDrag() const; + bool rangeZoom() const; + + // setters: + void setType(QCPAxis::AxisType type); + Q_SLOT void setDataRange(const QCPRange &dataRange); + Q_SLOT void setDataScaleType(QCPAxis::ScaleType scaleType); + Q_SLOT void setGradient(const QCPColorGradient &gradient); + void setLabel(const QString &str); + void setBarWidth(int width); + void setRangeDrag(bool enabled); + void setRangeZoom(bool enabled); + + // non-property methods: + QList colorMaps() const; + void rescaleDataRange(bool onlyVisibleMaps); + + // reimplemented virtual methods: + virtual void update(UpdatePhase phase); + +signals: + void dataRangeChanged(QCPRange newRange); + void dataScaleTypeChanged(QCPAxis::ScaleType scaleType); + void gradientChanged(QCPColorGradient newGradient); + +protected: + // property members: + QCPAxis::AxisType mType; + QCPRange mDataRange; + QCPAxis::ScaleType mDataScaleType; + QCPColorGradient mGradient; + int mBarWidth; + + // non-property members: + QPointer mAxisRect; + QPointer mColorAxis; + + // reimplemented virtual methods: + virtual void applyDefaultAntialiasingHint(QCPPainter *painter) const; + // events: + virtual void mousePressEvent(QMouseEvent *event); + virtual void mouseMoveEvent(QMouseEvent *event); + virtual void mouseReleaseEvent(QMouseEvent *event); + virtual void wheelEvent(QWheelEvent *event); + +private: + Q_DISABLE_COPY(QCPColorScale) + + friend class QCPColorScaleAxisRectPrivate; +}; + + +/*! \file */ + + + +class QCP_LIB_DECL QCPData +{ +public: + QCPData(); + QCPData(double key, double value); + double key, value; + double keyErrorPlus, keyErrorMinus; + double valueErrorPlus, valueErrorMinus; +}; +Q_DECLARE_TYPEINFO(QCPData, Q_MOVABLE_TYPE); + +/*! \typedef QCPDataMap + Container for storing \ref QCPData items in a sorted fashion. The key of the map + is the key member of the QCPData instance. + + This is the container in which QCPGraph holds its data. + \see QCPData, QCPGraph::setData +*/ +typedef QMap QCPDataMap; +typedef QMapIterator QCPDataMapIterator; +typedef QMutableMapIterator QCPDataMutableMapIterator; + + +class QCP_LIB_DECL QCPGraph : public QCPAbstractPlottable +{ + Q_OBJECT + /// \cond INCLUDE_QPROPERTIES + Q_PROPERTY(LineStyle lineStyle READ lineStyle WRITE setLineStyle) + Q_PROPERTY(QCPScatterStyle scatterStyle READ scatterStyle WRITE setScatterStyle) + Q_PROPERTY(ErrorType errorType READ errorType WRITE setErrorType) + Q_PROPERTY(QPen errorPen READ errorPen WRITE setErrorPen) + Q_PROPERTY(double errorBarSize READ errorBarSize WRITE setErrorBarSize) + Q_PROPERTY(bool errorBarSkipSymbol READ errorBarSkipSymbol WRITE setErrorBarSkipSymbol) + Q_PROPERTY(QCPGraph* channelFillGraph READ channelFillGraph WRITE setChannelFillGraph) + Q_PROPERTY(bool adaptiveSampling READ adaptiveSampling WRITE setAdaptiveSampling) + /// \endcond +public: + /*! + Defines how the graph's line is represented visually in the plot. The line is drawn with the + current pen of the graph (\ref setPen). + \see setLineStyle + */ + enum LineStyle { lsNone ///< data points are not connected with any lines (e.g. data only represented + ///< with symbols according to the scatter style, see \ref setScatterStyle) + ,lsLine ///< data points are connected by a straight line + ,lsStepLeft ///< line is drawn as steps where the step height is the value of the left data point + ,lsStepRight ///< line is drawn as steps where the step height is the value of the right data point + ,lsStepCenter ///< line is drawn as steps where the step is in between two data points + ,lsImpulse ///< each data point is represented by a line parallel to the value axis, which reaches from the data point to the zero-value-line + }; + Q_ENUMS(LineStyle) + /*! + Defines what kind of error bars are drawn for each data point + */ + enum ErrorType { etNone ///< No error bars are shown + ,etKey ///< Error bars for the key dimension of the data point are shown + ,etValue ///< Error bars for the value dimension of the data point are shown + ,etBoth ///< Error bars for both key and value dimensions of the data point are shown + }; + Q_ENUMS(ErrorType) + + explicit QCPGraph(QCPAxis *keyAxis, QCPAxis *valueAxis); + virtual ~QCPGraph(); + + // getters: + QCPDataMap *data() const { return mData; } + LineStyle lineStyle() const { return mLineStyle; } + QCPScatterStyle scatterStyle() const { return mScatterStyle; } + ErrorType errorType() const { return mErrorType; } + QPen errorPen() const { return mErrorPen; } + double errorBarSize() const { return mErrorBarSize; } + bool errorBarSkipSymbol() const { return mErrorBarSkipSymbol; } + QCPGraph *channelFillGraph() const { return mChannelFillGraph.data(); } + bool adaptiveSampling() const { return mAdaptiveSampling; } + + // setters: + void setData(QCPDataMap *data, bool copy=false); + void setData(const QVector &key, const QVector &value); + void setDataKeyError(const QVector &key, const QVector &value, const QVector &keyError); + void setDataKeyError(const QVector &key, const QVector &value, const QVector &keyErrorMinus, const QVector &keyErrorPlus); + void setDataValueError(const QVector &key, const QVector &value, const QVector &valueError); + void setDataValueError(const QVector &key, const QVector &value, const QVector &valueErrorMinus, const QVector &valueErrorPlus); + void setDataBothError(const QVector &key, const QVector &value, const QVector &keyError, const QVector &valueError); + void setDataBothError(const QVector &key, const QVector &value, const QVector &keyErrorMinus, const QVector &keyErrorPlus, const QVector &valueErrorMinus, const QVector &valueErrorPlus); + void setLineStyle(LineStyle ls); + void setScatterStyle(const QCPScatterStyle &style); + void setErrorType(ErrorType errorType); + void setErrorPen(const QPen &pen); + void setErrorBarSize(double size); + void setErrorBarSkipSymbol(bool enabled); + void setChannelFillGraph(QCPGraph *targetGraph); + void setAdaptiveSampling(bool enabled); + + // non-property methods: + void addData(const QCPDataMap &dataMap); + void addData(const QCPData &data); + void addData(double key, double value); + void addData(const QVector &keys, const QVector &values); + void removeDataBefore(double key); + void removeDataAfter(double key); + void removeData(double fromKey, double toKey); + void removeData(double key); + + // reimplemented virtual methods: + virtual void clearData(); + virtual double selectTest(const QPointF &pos, bool onlySelectable, QVariant *details=0) const; + using QCPAbstractPlottable::rescaleAxes; + using QCPAbstractPlottable::rescaleKeyAxis; + using QCPAbstractPlottable::rescaleValueAxis; + void rescaleAxes(bool onlyEnlarge, bool includeErrorBars) const; // overloads base class interface + void rescaleKeyAxis(bool onlyEnlarge, bool includeErrorBars) const; // overloads base class interface + void rescaleValueAxis(bool onlyEnlarge, bool includeErrorBars) const; // overloads base class interface + +protected: + // property members: + QCPDataMap *mData; + QPen mErrorPen; + LineStyle mLineStyle; + QCPScatterStyle mScatterStyle; + ErrorType mErrorType; + double mErrorBarSize; + bool mErrorBarSkipSymbol; + QPointer mChannelFillGraph; + bool mAdaptiveSampling; + + // reimplemented virtual methods: + virtual void draw(QCPPainter *painter); + virtual void drawLegendIcon(QCPPainter *painter, const QRectF &rect) const; + virtual QCPRange getKeyRange(bool &foundRange, SignDomain inSignDomain=sdBoth) const; + virtual QCPRange getValueRange(bool &foundRange, SignDomain inSignDomain=sdBoth) const; + virtual QCPRange getKeyRange(bool &foundRange, SignDomain inSignDomain, bool includeErrors) const; // overloads base class interface + virtual QCPRange getValueRange(bool &foundRange, SignDomain inSignDomain, bool includeErrors) const; // overloads base class interface + + // introduced virtual methods: + virtual void drawFill(QCPPainter *painter, QVector *lineData) const; + virtual void drawScatterPlot(QCPPainter *painter, QVector *scatterData) const; + virtual void drawLinePlot(QCPPainter *painter, QVector *lineData) const; + virtual void drawImpulsePlot(QCPPainter *painter, QVector *lineData) const; + + // non-virtual methods: + void getPreparedData(QVector *lineData, QVector *scatterData) const; + void getPlotData(QVector *lineData, QVector *scatterData) const; + void getScatterPlotData(QVector *scatterData) const; + void getLinePlotData(QVector *linePixelData, QVector *scatterData) const; + void getStepLeftPlotData(QVector *linePixelData, QVector *scatterData) const; + void getStepRightPlotData(QVector *linePixelData, QVector *scatterData) const; + void getStepCenterPlotData(QVector *linePixelData, QVector *scatterData) const; + void getImpulsePlotData(QVector *linePixelData, QVector *scatterData) const; + void drawError(QCPPainter *painter, double x, double y, const QCPData &data) const; + void getVisibleDataBounds(QCPDataMap::const_iterator &lower, QCPDataMap::const_iterator &upper) const; + int countDataInBounds(const QCPDataMap::const_iterator &lower, const QCPDataMap::const_iterator &upper, int maxCount) const; + void addFillBasePoints(QVector *lineData) const; + void removeFillBasePoints(QVector *lineData) const; + QPointF lowerFillBasePoint(double lowerKey) const; + QPointF upperFillBasePoint(double upperKey) const; + const QPolygonF getChannelFillPolygon(const QVector *lineData) const; + int findIndexBelowX(const QVector *data, double x) const; + int findIndexAboveX(const QVector *data, double x) const; + int findIndexBelowY(const QVector *data, double y) const; + int findIndexAboveY(const QVector *data, double y) const; + double pointDistance(const QPointF &pixelPoint) const; + + friend class QCustomPlot; + friend class QCPLegend; +}; + + +/*! \file */ + + + +class QCP_LIB_DECL QCPCurveData +{ +public: + QCPCurveData(); + QCPCurveData(double t, double key, double value); + double t, key, value; +}; +Q_DECLARE_TYPEINFO(QCPCurveData, Q_MOVABLE_TYPE); + +/*! \typedef QCPCurveDataMap + Container for storing \ref QCPCurveData items in a sorted fashion. The key of the map + is the t member of the QCPCurveData instance. + + This is the container in which QCPCurve holds its data. + \see QCPCurveData, QCPCurve::setData +*/ + +typedef QMap QCPCurveDataMap; +typedef QMapIterator QCPCurveDataMapIterator; +typedef QMutableMapIterator QCPCurveDataMutableMapIterator; + + +class QCP_LIB_DECL QCPCurve : public QCPAbstractPlottable +{ + Q_OBJECT + /// \cond INCLUDE_QPROPERTIES + Q_PROPERTY(QCPScatterStyle scatterStyle READ scatterStyle WRITE setScatterStyle) + Q_PROPERTY(LineStyle lineStyle READ lineStyle WRITE setLineStyle) + /// \endcond +public: + /*! + Defines how the curve's line is represented visually in the plot. The line is drawn with the + current pen of the curve (\ref setPen). + \see setLineStyle + */ + enum LineStyle { lsNone ///< No line is drawn between data points (e.g. only scatters) + ,lsLine ///< Data points are connected with a straight line + }; + explicit QCPCurve(QCPAxis *keyAxis, QCPAxis *valueAxis); + virtual ~QCPCurve(); + + // getters: + QCPCurveDataMap *data() const { return mData; } + QCPScatterStyle scatterStyle() const { return mScatterStyle; } + LineStyle lineStyle() const { return mLineStyle; } + + // setters: + void setData(QCPCurveDataMap *data, bool copy=false); + void setData(const QVector &t, const QVector &key, const QVector &value); + void setData(const QVector &key, const QVector &value); + void setScatterStyle(const QCPScatterStyle &style); + void setLineStyle(LineStyle style); + + // non-property methods: + void addData(const QCPCurveDataMap &dataMap); + void addData(const QCPCurveData &data); + void addData(double t, double key, double value); + void addData(double key, double value); + void addData(const QVector &ts, const QVector &keys, const QVector &values); + void removeDataBefore(double t); + void removeDataAfter(double t); + void removeData(double fromt, double tot); + void removeData(double t); + + // reimplemented virtual methods: + virtual void clearData(); + virtual double selectTest(const QPointF &pos, bool onlySelectable, QVariant *details=0) const; + +protected: + // property members: + QCPCurveDataMap *mData; + QCPScatterStyle mScatterStyle; + LineStyle mLineStyle; + + // reimplemented virtual methods: + virtual void draw(QCPPainter *painter); + virtual void drawLegendIcon(QCPPainter *painter, const QRectF &rect) const; + virtual QCPRange getKeyRange(bool &foundRange, SignDomain inSignDomain=sdBoth) const; + virtual QCPRange getValueRange(bool &foundRange, SignDomain inSignDomain=sdBoth) const; + + // introduced virtual methods: + virtual void drawScatterPlot(QCPPainter *painter, const QVector *pointData) const; + + // non-virtual methods: + void getCurveData(QVector *lineData) const; + int getRegion(double x, double y, double rectLeft, double rectTop, double rectRight, double rectBottom) const; + QPointF getOptimizedPoint(int prevRegion, double prevKey, double prevValue, double key, double value, double rectLeft, double rectTop, double rectRight, double rectBottom) const; + QVector getOptimizedCornerPoints(int prevRegion, int currentRegion, double prevKey, double prevValue, double key, double value, double rectLeft, double rectTop, double rectRight, double rectBottom) const; + bool mayTraverse(int prevRegion, int currentRegion) const; + bool getTraverse(double prevKey, double prevValue, double key, double value, double rectLeft, double rectTop, double rectRight, double rectBottom, QPointF &crossA, QPointF &crossB) const; + void getTraverseCornerPoints(int prevRegion, int currentRegion, double rectLeft, double rectTop, double rectRight, double rectBottom, QVector &beforeTraverse, QVector &afterTraverse) const; + double pointDistance(const QPointF &pixelPoint) const; + + friend class QCustomPlot; + friend class QCPLegend; +}; + + +/*! \file */ + + + +class QCP_LIB_DECL QCPBarsGroup : public QObject +{ + Q_OBJECT + /// \cond INCLUDE_QPROPERTIES + Q_PROPERTY(SpacingType spacingType READ spacingType WRITE setSpacingType) + Q_PROPERTY(double spacing READ spacing WRITE setSpacing) + /// \endcond +public: + /*! + Defines the ways the spacing between bars in the group can be specified. Thus it defines what + the number passed to \ref setSpacing actually means. + + \see setSpacingType, setSpacing + */ + enum SpacingType { stAbsolute ///< Bar spacing is in absolute pixels + ,stAxisRectRatio ///< Bar spacing is given by a fraction of the axis rect size + ,stPlotCoords ///< Bar spacing is in key coordinates and thus scales with the key axis range + }; + QCPBarsGroup(QCustomPlot *parentPlot); + ~QCPBarsGroup(); + + // getters: + SpacingType spacingType() const { return mSpacingType; } + double spacing() const { return mSpacing; } + + // setters: + void setSpacingType(SpacingType spacingType); + void setSpacing(double spacing); + + // non-virtual methods: + QList bars() const { return mBars; } + QCPBars* bars(int index) const; + int size() const { return mBars.size(); } + bool isEmpty() const { return mBars.isEmpty(); } + void clear(); + bool contains(QCPBars *bars) const { return mBars.contains(bars); } + void append(QCPBars *bars); + void insert(int i, QCPBars *bars); + void remove(QCPBars *bars); + +protected: + // non-property members: + QCustomPlot *mParentPlot; + SpacingType mSpacingType; + double mSpacing; + QList mBars; + + // non-virtual methods: + void registerBars(QCPBars *bars); + void unregisterBars(QCPBars *bars); + + // virtual methods: + double keyPixelOffset(const QCPBars *bars, double keyCoord); + double getPixelSpacing(const QCPBars *bars, double keyCoord); + +private: + Q_DISABLE_COPY(QCPBarsGroup) + + friend class QCPBars; +}; + + +class QCP_LIB_DECL QCPBarData +{ +public: + QCPBarData(); + QCPBarData(double key, double value); + double key, value; +}; +Q_DECLARE_TYPEINFO(QCPBarData, Q_MOVABLE_TYPE); + +/*! \typedef QCPBarDataMap + Container for storing \ref QCPBarData items in a sorted fashion. The key of the map + is the key member of the QCPBarData instance. + + This is the container in which QCPBars holds its data. + \see QCPBarData, QCPBars::setData +*/ +typedef QMap QCPBarDataMap; +typedef QMapIterator QCPBarDataMapIterator; +typedef QMutableMapIterator QCPBarDataMutableMapIterator; + + +class QCP_LIB_DECL QCPBars : public QCPAbstractPlottable +{ + Q_OBJECT + /// \cond INCLUDE_QPROPERTIES + Q_PROPERTY(double width READ width WRITE setWidth) + Q_PROPERTY(WidthType widthType READ widthType WRITE setWidthType) + Q_PROPERTY(QCPBarsGroup* barsGroup READ barsGroup WRITE setBarsGroup) + Q_PROPERTY(double baseValue READ baseValue WRITE setBaseValue) + Q_PROPERTY(QCPBars* barBelow READ barBelow) + Q_PROPERTY(QCPBars* barAbove READ barAbove) + /// \endcond +public: + /*! + Defines the ways the width of the bar can be specified. Thus it defines what the number passed + to \ref setWidth actually means. + + \see setWidthType, setWidth + */ + enum WidthType { wtAbsolute ///< Bar width is in absolute pixels + ,wtAxisRectRatio ///< Bar width is given by a fraction of the axis rect size + ,wtPlotCoords ///< Bar width is in key coordinates and thus scales with the key axis range + }; + Q_ENUMS(WidthType) + + explicit QCPBars(QCPAxis *keyAxis, QCPAxis *valueAxis); + virtual ~QCPBars(); + + // getters: + double width() const { return mWidth; } + WidthType widthType() const { return mWidthType; } + QCPBarsGroup *barsGroup() const { return mBarsGroup; } + double baseValue() const { return mBaseValue; } + QCPBars *barBelow() const { return mBarBelow.data(); } + QCPBars *barAbove() const { return mBarAbove.data(); } + QCPBarDataMap *data() const { return mData; } + + // setters: + void setWidth(double width); + void setWidthType(WidthType widthType); + void setBarsGroup(QCPBarsGroup *barsGroup); + void setBaseValue(double baseValue); + void setData(QCPBarDataMap *data, bool copy=false); + void setData(const QVector &key, const QVector &value); + + // non-property methods: + void moveBelow(QCPBars *bars); + void moveAbove(QCPBars *bars); + void addData(const QCPBarDataMap &dataMap); + void addData(const QCPBarData &data); + void addData(double key, double value); + void addData(const QVector &keys, const QVector &values); + void removeDataBefore(double key); + void removeDataAfter(double key); + void removeData(double fromKey, double toKey); + void removeData(double key); + + // reimplemented virtual methods: + virtual void clearData(); + virtual double selectTest(const QPointF &pos, bool onlySelectable, QVariant *details=0) const; + +protected: + // property members: + QCPBarDataMap *mData; + double mWidth; + WidthType mWidthType; + QCPBarsGroup *mBarsGroup; + double mBaseValue; + QPointer mBarBelow, mBarAbove; + + // reimplemented virtual methods: + virtual void draw(QCPPainter *painter); + virtual void drawLegendIcon(QCPPainter *painter, const QRectF &rect) const; + virtual QCPRange getKeyRange(bool &foundRange, SignDomain inSignDomain=sdBoth) const; + virtual QCPRange getValueRange(bool &foundRange, SignDomain inSignDomain=sdBoth) const; + + // non-virtual methods: + void getVisibleDataBounds(QCPBarDataMap::const_iterator &lower, QCPBarDataMap::const_iterator &upperEnd) const; + QPolygonF getBarPolygon(double key, double value) const; + void getPixelWidth(double key, double &lower, double &upper) const; + double getStackedBaseValue(double key, bool positive) const; + static void connectBars(QCPBars* lower, QCPBars* upper); + + friend class QCustomPlot; + friend class QCPLegend; + friend class QCPBarsGroup; +}; + + +/*! \file */ + + + +class QCP_LIB_DECL QCPStatisticalBox : public QCPAbstractPlottable +{ + Q_OBJECT + /// \cond INCLUDE_QPROPERTIES + Q_PROPERTY(double key READ key WRITE setKey) + Q_PROPERTY(double minimum READ minimum WRITE setMinimum) + Q_PROPERTY(double lowerQuartile READ lowerQuartile WRITE setLowerQuartile) + Q_PROPERTY(double median READ median WRITE setMedian) + Q_PROPERTY(double upperQuartile READ upperQuartile WRITE setUpperQuartile) + Q_PROPERTY(double maximum READ maximum WRITE setMaximum) + Q_PROPERTY(QVector outliers READ outliers WRITE setOutliers) + Q_PROPERTY(double width READ width WRITE setWidth) + Q_PROPERTY(double whiskerWidth READ whiskerWidth WRITE setWhiskerWidth) + Q_PROPERTY(QPen whiskerPen READ whiskerPen WRITE setWhiskerPen) + Q_PROPERTY(QPen whiskerBarPen READ whiskerBarPen WRITE setWhiskerBarPen) + Q_PROPERTY(QPen medianPen READ medianPen WRITE setMedianPen) + Q_PROPERTY(QCPScatterStyle outlierStyle READ outlierStyle WRITE setOutlierStyle) + /// \endcond +public: + explicit QCPStatisticalBox(QCPAxis *keyAxis, QCPAxis *valueAxis); + + // getters: + double key() const { return mKey; } + double minimum() const { return mMinimum; } + double lowerQuartile() const { return mLowerQuartile; } + double median() const { return mMedian; } + double upperQuartile() const { return mUpperQuartile; } + double maximum() const { return mMaximum; } + QVector outliers() const { return mOutliers; } + double width() const { return mWidth; } + double whiskerWidth() const { return mWhiskerWidth; } + QPen whiskerPen() const { return mWhiskerPen; } + QPen whiskerBarPen() const { return mWhiskerBarPen; } + QPen medianPen() const { return mMedianPen; } + QCPScatterStyle outlierStyle() const { return mOutlierStyle; } + + // setters: + void setKey(double key); + void setMinimum(double value); + void setLowerQuartile(double value); + void setMedian(double value); + void setUpperQuartile(double value); + void setMaximum(double value); + void setOutliers(const QVector &values); + void setData(double key, double minimum, double lowerQuartile, double median, double upperQuartile, double maximum); + void setWidth(double width); + void setWhiskerWidth(double width); + void setWhiskerPen(const QPen &pen); + void setWhiskerBarPen(const QPen &pen); + void setMedianPen(const QPen &pen); + void setOutlierStyle(const QCPScatterStyle &style); + + // non-property methods: + virtual void clearData(); + virtual double selectTest(const QPointF &pos, bool onlySelectable, QVariant *details=0) const; + +protected: + // property members: + QVector mOutliers; + double mKey, mMinimum, mLowerQuartile, mMedian, mUpperQuartile, mMaximum; + double mWidth; + double mWhiskerWidth; + QPen mWhiskerPen, mWhiskerBarPen, mMedianPen; + QCPScatterStyle mOutlierStyle; + + // reimplemented virtual methods: + virtual void draw(QCPPainter *painter); + virtual void drawLegendIcon(QCPPainter *painter, const QRectF &rect) const; + virtual QCPRange getKeyRange(bool &foundRange, SignDomain inSignDomain=sdBoth) const; + virtual QCPRange getValueRange(bool &foundRange, SignDomain inSignDomain=sdBoth) const; + + // introduced virtual methods: + virtual void drawQuartileBox(QCPPainter *painter, QRectF *quartileBox=0) const; + virtual void drawMedian(QCPPainter *painter) const; + virtual void drawWhiskers(QCPPainter *painter) const; + virtual void drawOutliers(QCPPainter *painter) const; + + friend class QCustomPlot; + friend class QCPLegend; +}; + + +class QCP_LIB_DECL QCPColorMapData +{ +public: + QCPColorMapData(int keySize, int valueSize, const QCPRange &keyRange, const QCPRange &valueRange); + ~QCPColorMapData(); + QCPColorMapData(const QCPColorMapData &other); + QCPColorMapData &operator=(const QCPColorMapData &other); + + // getters: + int keySize() const { return mKeySize; } + int valueSize() const { return mValueSize; } + QCPRange keyRange() const { return mKeyRange; } + QCPRange valueRange() const { return mValueRange; } + QCPRange dataBounds() const { return mDataBounds; } + double data(double key, double value); + double cell(int keyIndex, int valueIndex); + + // setters: + void setSize(int keySize, int valueSize); + void setKeySize(int keySize); + void setValueSize(int valueSize); + void setRange(const QCPRange &keyRange, const QCPRange &valueRange); + void setKeyRange(const QCPRange &keyRange); + void setValueRange(const QCPRange &valueRange); + void setData(double key, double value, double z); + void setCell(int keyIndex, int valueIndex, double z); + + // non-property methods: + void recalculateDataBounds(); + void clear(); + void fill(double z); + bool isEmpty() const { return mIsEmpty; } + void coordToCell(double key, double value, int *keyIndex, int *valueIndex) const; + void cellToCoord(int keyIndex, int valueIndex, double *key, double *value) const; + +protected: + // property members: + int mKeySize, mValueSize; + QCPRange mKeyRange, mValueRange; + bool mIsEmpty; + // non-property members: + double *mData; + QCPRange mDataBounds; + bool mDataModified; + + friend class QCPColorMap; +}; + + +class QCP_LIB_DECL QCPColorMap : public QCPAbstractPlottable +{ + Q_OBJECT + /// \cond INCLUDE_QPROPERTIES + Q_PROPERTY(QCPRange dataRange READ dataRange WRITE setDataRange NOTIFY dataRangeChanged) + Q_PROPERTY(QCPAxis::ScaleType dataScaleType READ dataScaleType WRITE setDataScaleType NOTIFY dataScaleTypeChanged) + Q_PROPERTY(QCPColorGradient gradient READ gradient WRITE setGradient NOTIFY gradientChanged) + Q_PROPERTY(bool interpolate READ interpolate WRITE setInterpolate) + Q_PROPERTY(bool tightBoundary READ tightBoundary WRITE setTightBoundary) + Q_PROPERTY(QCPColorScale* colorScale READ colorScale WRITE setColorScale) + /// \endcond +public: + explicit QCPColorMap(QCPAxis *keyAxis, QCPAxis *valueAxis); + virtual ~QCPColorMap(); + + // getters: + QCPColorMapData *data() const { return mMapData; } + QCPRange dataRange() const { return mDataRange; } + QCPAxis::ScaleType dataScaleType() const { return mDataScaleType; } + bool interpolate() const { return mInterpolate; } + bool tightBoundary() const { return mTightBoundary; } + QCPColorGradient gradient() const { return mGradient; } + QCPColorScale *colorScale() const { return mColorScale.data(); } + + // setters: + void setData(QCPColorMapData *data, bool copy=false); + Q_SLOT void setDataRange(const QCPRange &dataRange); + Q_SLOT void setDataScaleType(QCPAxis::ScaleType scaleType); + Q_SLOT void setGradient(const QCPColorGradient &gradient); + void setInterpolate(bool enabled); + void setTightBoundary(bool enabled); + void setColorScale(QCPColorScale *colorScale); + + // non-property methods: + void rescaleDataRange(bool recalculateDataBounds=false); + Q_SLOT void updateLegendIcon(Qt::TransformationMode transformMode=Qt::SmoothTransformation, const QSize &thumbSize=QSize(32, 18)); + + // reimplemented virtual methods: + virtual void clearData(); + virtual double selectTest(const QPointF &pos, bool onlySelectable, QVariant *details=0) const; + +signals: + void dataRangeChanged(QCPRange newRange); + void dataScaleTypeChanged(QCPAxis::ScaleType scaleType); + void gradientChanged(QCPColorGradient newGradient); + +protected: + // property members: + QCPRange mDataRange; + QCPAxis::ScaleType mDataScaleType; + QCPColorMapData *mMapData; + QCPColorGradient mGradient; + bool mInterpolate; + bool mTightBoundary; + QPointer mColorScale; + // non-property members: + QImage mMapImage, mUndersampledMapImage; + QPixmap mLegendIcon; + bool mMapImageInvalidated; + + // introduced virtual methods: + virtual void updateMapImage(); + + // reimplemented virtual methods: + virtual void draw(QCPPainter *painter); + virtual void drawLegendIcon(QCPPainter *painter, const QRectF &rect) const; + virtual QCPRange getKeyRange(bool &foundRange, SignDomain inSignDomain=sdBoth) const; + virtual QCPRange getValueRange(bool &foundRange, SignDomain inSignDomain=sdBoth) const; + + friend class QCustomPlot; + friend class QCPLegend; +}; + + +/*! \file */ + + + +class QCP_LIB_DECL QCPFinancialData +{ +public: + QCPFinancialData(); + QCPFinancialData(double key, double open, double high, double low, double close); + double key, open, high, low, close; +}; +Q_DECLARE_TYPEINFO(QCPFinancialData, Q_MOVABLE_TYPE); + +/*! \typedef QCPFinancialDataMap + Container for storing \ref QCPFinancialData items in a sorted fashion. The key of the map + is the key member of the QCPFinancialData instance. + + This is the container in which QCPFinancial holds its data. + \see QCPFinancial, QCPFinancial::setData +*/ +typedef QMap QCPFinancialDataMap; +typedef QMapIterator QCPFinancialDataMapIterator; +typedef QMutableMapIterator QCPFinancialDataMutableMapIterator; + + +class QCP_LIB_DECL QCPFinancial : public QCPAbstractPlottable +{ + Q_OBJECT + /// \cond INCLUDE_QPROPERTIES + Q_PROPERTY(ChartStyle chartStyle READ chartStyle WRITE setChartStyle) + Q_PROPERTY(double width READ width WRITE setWidth) + Q_PROPERTY(bool twoColored READ twoColored WRITE setTwoColored) + Q_PROPERTY(QBrush brushPositive READ brushPositive WRITE setBrushPositive) + Q_PROPERTY(QBrush brushNegative READ brushNegative WRITE setBrushNegative) + Q_PROPERTY(QPen penPositive READ penPositive WRITE setPenPositive) + Q_PROPERTY(QPen penNegative READ penNegative WRITE setPenNegative) + /// \endcond +public: + /*! + Defines the possible representations of OHLC data in the plot. + + \see setChartStyle + */ + enum ChartStyle { csOhlc ///< Open-High-Low-Close bar representation + ,csCandlestick ///< Candlestick representation + }; + Q_ENUMS(ChartStyle) + + explicit QCPFinancial(QCPAxis *keyAxis, QCPAxis *valueAxis); + virtual ~QCPFinancial(); + + // getters: + QCPFinancialDataMap *data() const { return mData; } + ChartStyle chartStyle() const { return mChartStyle; } + double width() const { return mWidth; } + bool twoColored() const { return mTwoColored; } + QBrush brushPositive() const { return mBrushPositive; } + QBrush brushNegative() const { return mBrushNegative; } + QPen penPositive() const { return mPenPositive; } + QPen penNegative() const { return mPenNegative; } + + + // setters: + void setData(QCPFinancialDataMap *data, bool copy=false); + void setData(const QVector &key, const QVector &open, const QVector &high, const QVector &low, const QVector &close); + void setChartStyle(ChartStyle style); + void setWidth(double width); + void setTwoColored(bool twoColored); + void setBrushPositive(const QBrush &brush); + void setBrushNegative(const QBrush &brush); + void setPenPositive(const QPen &pen); + void setPenNegative(const QPen &pen); + + // non-property methods: + void addData(const QCPFinancialDataMap &dataMap); + void addData(const QCPFinancialData &data); + void addData(double key, double open, double high, double low, double close); + void addData(const QVector &key, const QVector &open, const QVector &high, const QVector &low, const QVector &close); + void removeDataBefore(double key); + void removeDataAfter(double key); + void removeData(double fromKey, double toKey); + void removeData(double key); + + // reimplemented virtual methods: + virtual void clearData(); + virtual double selectTest(const QPointF &pos, bool onlySelectable, QVariant *details=0) const; + + // static methods: + static QCPFinancialDataMap timeSeriesToOhlc(const QVector &time, const QVector &value, double timeBinSize, double timeBinOffset = 0); + +protected: + // property members: + QCPFinancialDataMap *mData; + ChartStyle mChartStyle; + double mWidth; + bool mTwoColored; + QBrush mBrushPositive, mBrushNegative; + QPen mPenPositive, mPenNegative; + + // reimplemented virtual methods: + virtual void draw(QCPPainter *painter); + virtual void drawLegendIcon(QCPPainter *painter, const QRectF &rect) const; + virtual QCPRange getKeyRange(bool &foundRange, SignDomain inSignDomain=sdBoth) const; + virtual QCPRange getValueRange(bool &foundRange, SignDomain inSignDomain=sdBoth) const; + + // non-virtual methods: + void drawOhlcPlot(QCPPainter *painter, const QCPFinancialDataMap::const_iterator &begin, const QCPFinancialDataMap::const_iterator &end); + void drawCandlestickPlot(QCPPainter *painter, const QCPFinancialDataMap::const_iterator &begin, const QCPFinancialDataMap::const_iterator &end); + double ohlcSelectTest(const QPointF &pos, const QCPFinancialDataMap::const_iterator &begin, const QCPFinancialDataMap::const_iterator &end) const; + double candlestickSelectTest(const QPointF &pos, const QCPFinancialDataMap::const_iterator &begin, const QCPFinancialDataMap::const_iterator &end) const; + void getVisibleDataBounds(QCPFinancialDataMap::const_iterator &lower, QCPFinancialDataMap::const_iterator &upper) const; + + friend class QCustomPlot; + friend class QCPLegend; +}; + + +class QCP_LIB_DECL QCPItemStraightLine : public QCPAbstractItem +{ + Q_OBJECT + /// \cond INCLUDE_QPROPERTIES + Q_PROPERTY(QPen pen READ pen WRITE setPen) + Q_PROPERTY(QPen selectedPen READ selectedPen WRITE setSelectedPen) + /// \endcond +public: + QCPItemStraightLine(QCustomPlot *parentPlot); + virtual ~QCPItemStraightLine(); + + // getters: + QPen pen() const { return mPen; } + QPen selectedPen() const { return mSelectedPen; } + + // setters; + void setPen(const QPen &pen); + void setSelectedPen(const QPen &pen); + + // reimplemented virtual methods: + virtual double selectTest(const QPointF &pos, bool onlySelectable, QVariant *details=0) const; + + QCPItemPosition * const point1; + QCPItemPosition * const point2; + +protected: + // property members: + QPen mPen, mSelectedPen; + + // reimplemented virtual methods: + virtual void draw(QCPPainter *painter); + + // non-virtual methods: + double distToStraightLine(const QVector2D &point1, const QVector2D &vec, const QVector2D &point) const; + QLineF getRectClippedStraightLine(const QVector2D &point1, const QVector2D &vec, const QRect &rect) const; + QPen mainPen() const; +}; + + +class QCP_LIB_DECL QCPItemLine : public QCPAbstractItem +{ + Q_OBJECT + /// \cond INCLUDE_QPROPERTIES + Q_PROPERTY(QPen pen READ pen WRITE setPen) + Q_PROPERTY(QPen selectedPen READ selectedPen WRITE setSelectedPen) + Q_PROPERTY(QCPLineEnding head READ head WRITE setHead) + Q_PROPERTY(QCPLineEnding tail READ tail WRITE setTail) + /// \endcond +public: + QCPItemLine(QCustomPlot *parentPlot); + virtual ~QCPItemLine(); + + // getters: + QPen pen() const { return mPen; } + QPen selectedPen() const { return mSelectedPen; } + QCPLineEnding head() const { return mHead; } + QCPLineEnding tail() const { return mTail; } + + // setters; + void setPen(const QPen &pen); + void setSelectedPen(const QPen &pen); + void setHead(const QCPLineEnding &head); + void setTail(const QCPLineEnding &tail); + + // reimplemented virtual methods: + virtual double selectTest(const QPointF &pos, bool onlySelectable, QVariant *details=0) const; + + QCPItemPosition * const start; + QCPItemPosition * const end; + +protected: + // property members: + QPen mPen, mSelectedPen; + QCPLineEnding mHead, mTail; + + // reimplemented virtual methods: + virtual void draw(QCPPainter *painter); + + // non-virtual methods: + QLineF getRectClippedLine(const QVector2D &start, const QVector2D &end, const QRect &rect) const; + QPen mainPen() const; +}; + + +class QCP_LIB_DECL QCPItemCurve : public QCPAbstractItem +{ + Q_OBJECT + /// \cond INCLUDE_QPROPERTIES + Q_PROPERTY(QPen pen READ pen WRITE setPen) + Q_PROPERTY(QPen selectedPen READ selectedPen WRITE setSelectedPen) + Q_PROPERTY(QCPLineEnding head READ head WRITE setHead) + Q_PROPERTY(QCPLineEnding tail READ tail WRITE setTail) + /// \endcond +public: + QCPItemCurve(QCustomPlot *parentPlot); + virtual ~QCPItemCurve(); + + // getters: + QPen pen() const { return mPen; } + QPen selectedPen() const { return mSelectedPen; } + QCPLineEnding head() const { return mHead; } + QCPLineEnding tail() const { return mTail; } + + // setters; + void setPen(const QPen &pen); + void setSelectedPen(const QPen &pen); + void setHead(const QCPLineEnding &head); + void setTail(const QCPLineEnding &tail); + + // reimplemented virtual methods: + virtual double selectTest(const QPointF &pos, bool onlySelectable, QVariant *details=0) const; + + QCPItemPosition * const start; + QCPItemPosition * const startDir; + QCPItemPosition * const endDir; + QCPItemPosition * const end; + +protected: + // property members: + QPen mPen, mSelectedPen; + QCPLineEnding mHead, mTail; + + // reimplemented virtual methods: + virtual void draw(QCPPainter *painter); + + // non-virtual methods: + QPen mainPen() const; +}; + + +class QCP_LIB_DECL QCPItemRect : public QCPAbstractItem +{ + Q_OBJECT + /// \cond INCLUDE_QPROPERTIES + Q_PROPERTY(QPen pen READ pen WRITE setPen) + Q_PROPERTY(QPen selectedPen READ selectedPen WRITE setSelectedPen) + Q_PROPERTY(QBrush brush READ brush WRITE setBrush) + Q_PROPERTY(QBrush selectedBrush READ selectedBrush WRITE setSelectedBrush) + /// \endcond +public: + QCPItemRect(QCustomPlot *parentPlot); + virtual ~QCPItemRect(); + + // getters: + QPen pen() const { return mPen; } + QPen selectedPen() const { return mSelectedPen; } + QBrush brush() const { return mBrush; } + QBrush selectedBrush() const { return mSelectedBrush; } + + // setters; + void setPen(const QPen &pen); + void setSelectedPen(const QPen &pen); + void setBrush(const QBrush &brush); + void setSelectedBrush(const QBrush &brush); + + // reimplemented virtual methods: + virtual double selectTest(const QPointF &pos, bool onlySelectable, QVariant *details=0) const; + + QCPItemPosition * const topLeft; + QCPItemPosition * const bottomRight; + QCPItemAnchor * const top; + QCPItemAnchor * const topRight; + QCPItemAnchor * const right; + QCPItemAnchor * const bottom; + QCPItemAnchor * const bottomLeft; + QCPItemAnchor * const left; + +protected: + enum AnchorIndex {aiTop, aiTopRight, aiRight, aiBottom, aiBottomLeft, aiLeft}; + + // property members: + QPen mPen, mSelectedPen; + QBrush mBrush, mSelectedBrush; + + // reimplemented virtual methods: + virtual void draw(QCPPainter *painter); + virtual QPointF anchorPixelPoint(int anchorId) const; + + // non-virtual methods: + QPen mainPen() const; + QBrush mainBrush() const; +}; + + +class QCP_LIB_DECL QCPItemText : public QCPAbstractItem +{ + Q_OBJECT + /// \cond INCLUDE_QPROPERTIES + Q_PROPERTY(QColor color READ color WRITE setColor) + Q_PROPERTY(QColor selectedColor READ selectedColor WRITE setSelectedColor) + Q_PROPERTY(QPen pen READ pen WRITE setPen) + Q_PROPERTY(QPen selectedPen READ selectedPen WRITE setSelectedPen) + Q_PROPERTY(QBrush brush READ brush WRITE setBrush) + Q_PROPERTY(QBrush selectedBrush READ selectedBrush WRITE setSelectedBrush) + Q_PROPERTY(QFont font READ font WRITE setFont) + Q_PROPERTY(QFont selectedFont READ selectedFont WRITE setSelectedFont) + Q_PROPERTY(QString text READ text WRITE setText) + Q_PROPERTY(Qt::Alignment positionAlignment READ positionAlignment WRITE setPositionAlignment) + Q_PROPERTY(Qt::Alignment textAlignment READ textAlignment WRITE setTextAlignment) + Q_PROPERTY(double rotation READ rotation WRITE setRotation) + Q_PROPERTY(QMargins padding READ padding WRITE setPadding) + /// \endcond +public: + QCPItemText(QCustomPlot *parentPlot); + virtual ~QCPItemText(); + + // getters: + QColor color() const { return mColor; } + QColor selectedColor() const { return mSelectedColor; } + QPen pen() const { return mPen; } + QPen selectedPen() const { return mSelectedPen; } + QBrush brush() const { return mBrush; } + QBrush selectedBrush() const { return mSelectedBrush; } + QFont font() const { return mFont; } + QFont selectedFont() const { return mSelectedFont; } + QString text() const { return mText; } + Qt::Alignment positionAlignment() const { return mPositionAlignment; } + Qt::Alignment textAlignment() const { return mTextAlignment; } + double rotation() const { return mRotation; } + QMargins padding() const { return mPadding; } + + // setters; + void setColor(const QColor &color); + void setSelectedColor(const QColor &color); + void setPen(const QPen &pen); + void setSelectedPen(const QPen &pen); + void setBrush(const QBrush &brush); + void setSelectedBrush(const QBrush &brush); + void setFont(const QFont &font); + void setSelectedFont(const QFont &font); + void setText(const QString &text); + void setPositionAlignment(Qt::Alignment alignment); + void setTextAlignment(Qt::Alignment alignment); + void setRotation(double degrees); + void setPadding(const QMargins &padding); + + // reimplemented virtual methods: + virtual double selectTest(const QPointF &pos, bool onlySelectable, QVariant *details=0) const; + + QCPItemPosition * const position; + QCPItemAnchor * const topLeft; + QCPItemAnchor * const top; + QCPItemAnchor * const topRight; + QCPItemAnchor * const right; + QCPItemAnchor * const bottomRight; + QCPItemAnchor * const bottom; + QCPItemAnchor * const bottomLeft; + QCPItemAnchor * const left; + +protected: + enum AnchorIndex {aiTopLeft, aiTop, aiTopRight, aiRight, aiBottomRight, aiBottom, aiBottomLeft, aiLeft}; + + // property members: + QColor mColor, mSelectedColor; + QPen mPen, mSelectedPen; + QBrush mBrush, mSelectedBrush; + QFont mFont, mSelectedFont; + QString mText; + Qt::Alignment mPositionAlignment; + Qt::Alignment mTextAlignment; + double mRotation; + QMargins mPadding; + + // reimplemented virtual methods: + virtual void draw(QCPPainter *painter); + virtual QPointF anchorPixelPoint(int anchorId) const; + + // non-virtual methods: + QPointF getTextDrawPoint(const QPointF &pos, const QRectF &rect, Qt::Alignment positionAlignment) const; + QFont mainFont() const; + QColor mainColor() const; + QPen mainPen() const; + QBrush mainBrush() const; +}; + + +class QCP_LIB_DECL QCPItemEllipse : public QCPAbstractItem +{ + Q_OBJECT + /// \cond INCLUDE_QPROPERTIES + Q_PROPERTY(QPen pen READ pen WRITE setPen) + Q_PROPERTY(QPen selectedPen READ selectedPen WRITE setSelectedPen) + Q_PROPERTY(QBrush brush READ brush WRITE setBrush) + Q_PROPERTY(QBrush selectedBrush READ selectedBrush WRITE setSelectedBrush) + /// \endcond +public: + QCPItemEllipse(QCustomPlot *parentPlot); + virtual ~QCPItemEllipse(); + + // getters: + QPen pen() const { return mPen; } + QPen selectedPen() const { return mSelectedPen; } + QBrush brush() const { return mBrush; } + QBrush selectedBrush() const { return mSelectedBrush; } + + // setters; + void setPen(const QPen &pen); + void setSelectedPen(const QPen &pen); + void setBrush(const QBrush &brush); + void setSelectedBrush(const QBrush &brush); + + // reimplemented virtual methods: + virtual double selectTest(const QPointF &pos, bool onlySelectable, QVariant *details=0) const; + + QCPItemPosition * const topLeft; + QCPItemPosition * const bottomRight; + QCPItemAnchor * const topLeftRim; + QCPItemAnchor * const top; + QCPItemAnchor * const topRightRim; + QCPItemAnchor * const right; + QCPItemAnchor * const bottomRightRim; + QCPItemAnchor * const bottom; + QCPItemAnchor * const bottomLeftRim; + QCPItemAnchor * const left; + QCPItemAnchor * const center; + +protected: + enum AnchorIndex {aiTopLeftRim, aiTop, aiTopRightRim, aiRight, aiBottomRightRim, aiBottom, aiBottomLeftRim, aiLeft, aiCenter}; + + // property members: + QPen mPen, mSelectedPen; + QBrush mBrush, mSelectedBrush; + + // reimplemented virtual methods: + virtual void draw(QCPPainter *painter); + virtual QPointF anchorPixelPoint(int anchorId) const; + + // non-virtual methods: + QPen mainPen() const; + QBrush mainBrush() const; +}; + + +class QCP_LIB_DECL QCPItemPixmap : public QCPAbstractItem +{ + Q_OBJECT + /// \cond INCLUDE_QPROPERTIES + Q_PROPERTY(QPixmap pixmap READ pixmap WRITE setPixmap) + Q_PROPERTY(bool scaled READ scaled WRITE setScaled) + Q_PROPERTY(Qt::AspectRatioMode aspectRatioMode READ aspectRatioMode) + Q_PROPERTY(Qt::TransformationMode transformationMode READ transformationMode) + Q_PROPERTY(QPen pen READ pen WRITE setPen) + Q_PROPERTY(QPen selectedPen READ selectedPen WRITE setSelectedPen) + /// \endcond +public: + QCPItemPixmap(QCustomPlot *parentPlot); + virtual ~QCPItemPixmap(); + + // getters: + QPixmap pixmap() const { return mPixmap; } + bool scaled() const { return mScaled; } + Qt::AspectRatioMode aspectRatioMode() const { return mAspectRatioMode; } + Qt::TransformationMode transformationMode() const { return mTransformationMode; } + QPen pen() const { return mPen; } + QPen selectedPen() const { return mSelectedPen; } + + // setters; + void setPixmap(const QPixmap &pixmap); + void setScaled(bool scaled, Qt::AspectRatioMode aspectRatioMode=Qt::KeepAspectRatio, Qt::TransformationMode transformationMode=Qt::SmoothTransformation); + void setPen(const QPen &pen); + void setSelectedPen(const QPen &pen); + + // reimplemented virtual methods: + virtual double selectTest(const QPointF &pos, bool onlySelectable, QVariant *details=0) const; + + QCPItemPosition * const topLeft; + QCPItemPosition * const bottomRight; + QCPItemAnchor * const top; + QCPItemAnchor * const topRight; + QCPItemAnchor * const right; + QCPItemAnchor * const bottom; + QCPItemAnchor * const bottomLeft; + QCPItemAnchor * const left; + +protected: + enum AnchorIndex {aiTop, aiTopRight, aiRight, aiBottom, aiBottomLeft, aiLeft}; + + // property members: + QPixmap mPixmap; + QPixmap mScaledPixmap; + bool mScaled; + bool mScaledPixmapInvalidated; + Qt::AspectRatioMode mAspectRatioMode; + Qt::TransformationMode mTransformationMode; + QPen mPen, mSelectedPen; + + // reimplemented virtual methods: + virtual void draw(QCPPainter *painter); + virtual QPointF anchorPixelPoint(int anchorId) const; + + // non-virtual methods: + void updateScaledPixmap(QRect finalRect=QRect(), bool flipHorz=false, bool flipVert=false); + QRect getFinalRect(bool *flippedHorz=0, bool *flippedVert=0) const; + QPen mainPen() const; +}; + + +class QCP_LIB_DECL QCPItemTracer : public QCPAbstractItem +{ + Q_OBJECT + /// \cond INCLUDE_QPROPERTIES + Q_PROPERTY(QPen pen READ pen WRITE setPen) + Q_PROPERTY(QPen selectedPen READ selectedPen WRITE setSelectedPen) + Q_PROPERTY(QBrush brush READ brush WRITE setBrush) + Q_PROPERTY(QBrush selectedBrush READ selectedBrush WRITE setSelectedBrush) + Q_PROPERTY(double size READ size WRITE setSize) + Q_PROPERTY(TracerStyle style READ style WRITE setStyle) + Q_PROPERTY(QCPGraph* graph READ graph WRITE setGraph) + Q_PROPERTY(double graphKey READ graphKey WRITE setGraphKey) + Q_PROPERTY(bool interpolating READ interpolating WRITE setInterpolating) + /// \endcond +public: + /*! + The different visual appearances a tracer item can have. Some styles size may be controlled with \ref setSize. + + \see setStyle + */ + enum TracerStyle { tsNone ///< The tracer is not visible + ,tsPlus ///< A plus shaped crosshair with limited size + ,tsCrosshair ///< A plus shaped crosshair which spans the complete axis rect + ,tsCircle ///< A circle + ,tsSquare ///< A square + }; + Q_ENUMS(TracerStyle) + + QCPItemTracer(QCustomPlot *parentPlot); + virtual ~QCPItemTracer(); + + // getters: + QPen pen() const { return mPen; } + QPen selectedPen() const { return mSelectedPen; } + QBrush brush() const { return mBrush; } + QBrush selectedBrush() const { return mSelectedBrush; } + double size() const { return mSize; } + TracerStyle style() const { return mStyle; } + QCPGraph *graph() const { return mGraph; } + double graphKey() const { return mGraphKey; } + bool interpolating() const { return mInterpolating; } + + // setters; + void setPen(const QPen &pen); + void setSelectedPen(const QPen &pen); + void setBrush(const QBrush &brush); + void setSelectedBrush(const QBrush &brush); + void setSize(double size); + void setStyle(TracerStyle style); + void setGraph(QCPGraph *graph); + void setGraphKey(double key); + void setInterpolating(bool enabled); + + // reimplemented virtual methods: + virtual double selectTest(const QPointF &pos, bool onlySelectable, QVariant *details=0) const; + + // non-virtual methods: + void updatePosition(); + + QCPItemPosition * const position; + +protected: + // property members: + QPen mPen, mSelectedPen; + QBrush mBrush, mSelectedBrush; + double mSize; + TracerStyle mStyle; + QCPGraph *mGraph; + double mGraphKey; + bool mInterpolating; + + // reimplemented virtual methods: + virtual void draw(QCPPainter *painter); + + // non-virtual methods: + QPen mainPen() const; + QBrush mainBrush() const; +}; + + +class QCP_LIB_DECL QCPItemBracket : public QCPAbstractItem +{ + Q_OBJECT + /// \cond INCLUDE_QPROPERTIES + Q_PROPERTY(QPen pen READ pen WRITE setPen) + Q_PROPERTY(QPen selectedPen READ selectedPen WRITE setSelectedPen) + Q_PROPERTY(double length READ length WRITE setLength) + Q_PROPERTY(BracketStyle style READ style WRITE setStyle) + /// \endcond +public: + enum BracketStyle { bsSquare ///< A brace with angled edges + ,bsRound ///< A brace with round edges + ,bsCurly ///< A curly brace + ,bsCalligraphic ///< A curly brace with varying stroke width giving a calligraphic impression + }; + + QCPItemBracket(QCustomPlot *parentPlot); + virtual ~QCPItemBracket(); + + // getters: + QPen pen() const { return mPen; } + QPen selectedPen() const { return mSelectedPen; } + double length() const { return mLength; } + BracketStyle style() const { return mStyle; } + + // setters; + void setPen(const QPen &pen); + void setSelectedPen(const QPen &pen); + void setLength(double length); + void setStyle(BracketStyle style); + + // reimplemented virtual methods: + virtual double selectTest(const QPointF &pos, bool onlySelectable, QVariant *details=0) const; + + QCPItemPosition * const left; + QCPItemPosition * const right; + QCPItemAnchor * const center; + +protected: + // property members: + enum AnchorIndex {aiCenter}; + QPen mPen, mSelectedPen; + double mLength; + BracketStyle mStyle; + + // reimplemented virtual methods: + virtual void draw(QCPPainter *painter); + virtual QPointF anchorPixelPoint(int anchorId) const; + + // non-virtual methods: + QPen mainPen() const; +}; + +#endif // QCUSTOMPLOT_H + diff --git a/Analysis/DataViewer/qml.qrc b/Analysis/DataViewer/qml.qrc new file mode 100644 index 00000000..7684346a --- /dev/null +++ b/Analysis/DataViewer/qml.qrc @@ -0,0 +1,6 @@ + + + main.qml + MainForm.ui.qml + + diff --git a/Analysis/Image/AdvCompr.cpp b/Analysis/Image/AdvCompr.cpp index a5a473b2..22a0bea3 100755 --- a/Analysis/Image/AdvCompr.cpp +++ b/Analysis/Image/AdvCompr.cpp @@ -4,13 +4,14 @@ #include "AdvCompr.h" #ifndef WIN32 #include "PCACompression.h" +#ifdef PCASPLINE #include "PcaSpline.h" +#endif #include "Utils.h" #endif //#define SMOOTH_BEGINNING_OF_TRACE 1 //#define POST_CLEAR_FRONT_PORCH 1 -//#define PCA_MEAN_SMOOTHING 3 //#define PCA_CONVERGE_BASIS_VECTORS 1 //#define SCALE_TRACES 1 @@ -23,16 +24,22 @@ //#define USE_LESS_BASIS_VECTS 3 //#define OUTPUT_VECTOR_COEFFS 1 //#define CLEAR_MEAN 1 -//#define CENTER_TRACES 1 -#define XTALK_FRAC (0.20f) -#define XTALK_FRAC_MODE73 (0.30f) -#define XTALK_FRAC_MODE76 (0.25f) +#ifndef RDR_MAX_REGIONS +#define RDR_MAX_REGIONS 96 +#endif + + +#define XTALK_FRAC (0.30f) #define XTALK_FRAC_EARLY 1 #define GAIN_CORR_IN_ADVTEST 1 +#define NOT_PINNED(idx) (mMask == NULL || (mMask[(idx)])) +#define V8_SUM(a) (a.A[0]+a.A[1]+a.A[2]+a.A[3]+a.A[4]+a.A[5]+a.A[6]+a.A[7]) +#define SIGS_ACC(idx,frame) (mCorr_sigs[((idx)*(npts) + (frame))]) +#define NOISE_ACC(idx,frame) (mCorr_noise[((idx)*(npts) + (frame))]) // limits on the gain term we allow @@ -56,13 +63,13 @@ uint32_t AdvCompr::threadMemLen[ADVC_MAX_REGIONS] = {0}; AdvCompr::AdvCompr(FILE_HANDLE _fd, short int *_raw, int _w, int _h, int _nImgPts, int _nUncompImgPts, int *_timestamps_compr, int *_timestamps_uncompr, int _testUncompr, char *_fname, - char *_options, int _frameRate) + char *_options, int _frameRate, int RemoveRowNoise) { ThreadNum=-1; GblMemPtr=NULL; GblMemLen=0; - do_cnc = true; - do_rnc = true; + do_cnc = false;//true; +// do_rnc = true; w = _w; h = _h; npts = _nImgPts; @@ -78,11 +85,12 @@ AdvCompr::AdvCompr(FILE_HANDLE _fd, short int *_raw, int _w, int _h, nPcaSplineBasisVectors=0; nDfcCoeff=0; frameRate=_frameRate; + CorrectRowNoise = RemoveRowNoise; nBasisVectors=0; SplineStrategy[0]=0; - options=_options; + inp_options=_options; if (_fname) strncpy(fname, _fname, sizeof(fname)); @@ -114,61 +122,6 @@ AdvCompr::~AdvCompr() #ifndef WIN32 -void AdvCompr::ZeroTraceMean(short int *raw, int h, int w, int npts) -{ - int frameStride=w*h; - - // first, zero all the trace means - for(int y=0;y 1000){ - start_ts = ts; - } - if(end_ts == 0 && timestamps_compr[ts] > 3000){ - end_ts = ts; - } - } - - - for(int idx=0;idx0){ lsum[y*w+x] =lsum[(y-1)*w+x] + rptr[y*w+x]; lsumNum[y*w+x]=lsumNum[(y-1)*w+x] + 1; @@ -202,7 +155,7 @@ void AdvCompr::NeighborSubtract(short int *raw, int h, int w, int npts, float *s lsum[y*w+x]=0; } for(x=1;x0){ lsum[y*w+x]=lsum[(y-1)*w+x] + lsum[y*w+x-1] - lsum[(y-1)*w+x-1] + rptr[y*w+x]; lsumNum[y*w+x]=lsumNum[(y-1)*w+x] + lsumNum[y*w+x-1] - lsumNum[(y-1)*w+x-1] + 1; @@ -229,7 +182,7 @@ void AdvCompr::NeighborSubtract(short int *raw, int h, int w, int npts, float *s // now, neighbor subtract each well for(int y=0;y= 0) Write(fd, &FileHdrV4, sizeof(FileHdrV4)); @@ -354,44 +324,41 @@ int AdvCompr::Compress(int _threadNum, uint32_t region, int targetAvg) Write(fd, timestamps_newfr, sizeof(timestamps_newfr[0]) * npts_newfr); } + double start_xtc=AdvCTimer(); int total_iter = 0; -#ifdef SCALE_TRACES - ComputeGain_FullChip(); + +#ifdef XTALK_FRAC_EARLY + findPinned(); + if(CorrectRowNoise) + ApplyGain_FullChip_xtalkcorr_sumRows(XTALK_FRAC, w, h, npts, (short unsigned int *)raw); + else + ApplyGain_FullChip_xtalkcorr(XTALK_FRAC, w, h, npts, (short unsigned int *)raw); + +#else + ApplyGain_FullChip(0); #endif - ApplyGain_FullChip(/*do_rnc?0:*/targetAvg); + timing.xtalk += AdvCTimer() - start_xtc; + if(CorrectRowNoise){ + if(region >= RDR_MAX_REGIONS) + NNSubtractComparatorSigs_tn(100); + else + NNSubtractComparatorSigs(500); + } +#if 0 #ifndef PYEXT - if(do_rnc) { +// if(do_rnc) + { double start2 = AdvCTimer(); - CorrNoiseCorrector rnc; - rnc.CorrectCorrNoise(raw, h, w, npts, 3, (region >= 96), 0,0,ThreadNum,targetAvg); + rnc.CorrectCorrNoise(raw, h, w, npts, 1, (region >= 96), 0,0,ThreadNum,targetAvg); timing.rcn += AdvCTimer() - start2; - -#ifdef XTALK_FRAC_EARLY - { - float corrFrac=XTALK_FRAC; -#ifdef BB_DC - if(fpgaImage.ChipMode == 73) - corrFrac=XTALK_FRAC_MODE73; - if(fpgaImage.ChipMode == 76) - corrFrac=XTALK_FRAC_MODE76; + } #endif - xtalkCorrect_raw(corrFrac, w, h, npts, raw); - } #endif - } - else if (do_cnc) { - double start2 = AdvCTimer(); - ComparatorNoiseCorrector cnc; - cnc.CorrectComparatorNoise(raw, h, w, npts, NULL, 0, true, - false, ThreadNum); - timing.ccn += AdvCTimer() - start2; - memcpy(mMask,raw,w*h*sizeof(raw[0])); // use the first frame as a pinned mask - } -#endif + for (int rblk = 0; rblk < rblocks; rblk++) { @@ -443,64 +410,19 @@ int AdvCompr::Compress(int _threadNum, uint32_t region, int targetAvg) t0est = findt0(&t0estIdx); // printf("t0est=%d t0estIdx=%d\n",t0est,t0estIdx); -#ifdef SMOOTH_BEGINNING_OF_TRACE - smoothBeginning(); // locates the traces better. -#endif - - SetMeanOfFramesToZero_SubMean(npts); // subtracts mean as well -#ifdef CLEAR_BEGINNING_OF_TRACE -// float tmpMean[npts]; -// memcpy(tmpMean,mean_trc,sizeof(float)*npts); -// memset(mean_trc,0,sizeof(float)*npts); -// SetMeanOfFramesToZero_SubMean(npts); // subtracts mean as well -// memcpy(mean_trc,tmpMean,sizeof(float)*npts); - ClearBeginningOfTrace(); -#endif - - SmoothMean(); - -#ifdef CLEAR_MEAN - for(int pt=0;pt= 0) WriteTraceBlock(); @@ -519,51 +441,6 @@ int AdvCompr::Compress(int _threadNum, uint32_t region, int targetAvg) return total_iter; } -void AdvCompr::SmoothMean() -{ -#ifdef PCA_MEAN_SMOOTHING - - float nmean_trc[hdr.npts]; - for (int pt = 0; pt < hdr.npts; pt++){ - int span=PCA_MEAN_SMOOTHING; - int start_pt=pt-span; - int end_pt=pt+span; - if(start_pt<0) - start_pt=0; - if(end_pt>hdr.npts) - end_pt=hdr.npts; - - float avg=0; - for(int npt=start_pt;npt 15) - end = 15; -// v8s zeroV=LD_VEC8S(0); -// int frameStride=w*h; - - // mean has already been subtracted, so just zero the points up to t0est - for(int pt=0;pt mean_highest_point){ - mean_highest_point = mean_trc[pt]; - highest_pt = pt; - } - } -#else // use the last point as the height highest_pt=npts-1; lowest_pt=1; mean_highest_point=mean_trc[highest_pt]; mean_lowest_point = mean_trc[lowest_pt]; -#endif float mean_height=mean_highest_point - mean_lowest_point; @@ -708,200 +551,12 @@ void AdvCompr::ComputeGain_FullChip() // } } -void AdvCompr::adjustForDrift() -{ - // create a mean trace - int frameStride=w*h; - float meanTrc[npts]; - short int meanTrcSub[npts]; - uint64_t sum=0; - int pt=0; - - for (pt=0;pt 1000) - break; - } - int onesec_ts_pt = pt; - float onesec_ts = timestamps_compr[pt]; - float slope = (meanTrc[pt]-meanTrc[0])/((float)timestamps_compr[pt]); - - for(pt=0;pt 1000) -// break; -// } -// float onesec_ts = timestamps_compr[pt]; -// -// // we now have the last timestamp before the valve opens -// // this should be a flat region -// float slope = (mean_trc[pt]-mean_trc[0])/((float)timestamps_compr[pt]); -// -//// printf("B Slope=%f lastpt=%d %.0f %.0f %.0f %.0f %.0f\n",slope,pt,mean_trc[0],mean_trc[1],mean_trc[2],mean_trc[3],mean_trc[4]); -// -// for(pt=0;pt 12) - end_pt=12; - // we already have t0est... - float weight[npts]; - float totalWeight=0; - int prev=0; - for (int i = 0; i < end_pt && i < npts; i++){ - // get how many samples went into this timepoint - weight[i] = (timestamps_compr[i] - prev) + 10 / timestamps_compr[0]; - totalWeight += weight[i]; - prev = timestamps_compr[i]; - } - - // smooth from 0 to end_ts - for (int nt = 0; nt < ntrcs; nt++){ - float avg=0; - for (int pt = 0; pt < end_pt; pt++){ - avg += ((float)TRCS_ACCESS(nt,pt))*weight[pt]; - } - avg /= totalWeight; - short int avgS = avg; - for (int pt = 0; pt < end_pt; pt++){ - TRCS_ACCESS(nt,pt) = avgS; - } - } - // now, smooth the mean trc - { - float avg=0; - for (int pt = 0; pt < end_pt; pt++){ - avg += mean_trc[pt]*weight[pt]; - } - avg /= totalWeight; - short int avgS = avg; - for (int pt = 0; pt < end_pt; pt++){ - mean_trc[pt] = avgS; - } - } - -} - -void AdvCompr::PostClearFrontPorch() -{ - // first, figure out where t0 should really be.. - int num_interesting_nvect = 3; - - int t0IdxEst[10]={0}; - float t0=0; - int t0Int=0; - - if(hdr.nBasisVec < num_interesting_nvect) - num_interesting_nvect = hdr.nBasisVec; - - for (int nvect = 0; nvect < num_interesting_nvect; nvect++) - { - float maxVal = 0; - for (int pt = 0; pt < npts; pt++){ - float val = fabs(COEFF_ACCESS(pt,nvect)-COEFF_ACCESS(0,nvect)); - if(val > maxVal) - maxVal = val; - } - float triggerLevel = maxVal/10.0f; -// printf("%s: vect %d maxVal %.2f triggerLeve = %.2f (",__FUNCTION__,nvect,maxVal,triggerLevel); -// -// for (int pt = 0; pt < npts; pt++){ -// printf(" %.2f",COEFF_ACCESS(pt,nvect)-COEFF_ACCESS(0,nvect)); -// } -// printf(")\n"); - - for (int pt = 0; pt < npts; pt++){ - if(fabs(COEFF_ACCESS(pt,nvect)-COEFF_ACCESS(0,nvect)) > triggerLevel){ - t0IdxEst[nvect] = pt; -// printf("%s: vect %d --> %d\n",__FUNCTION__,nvect,t0IdxEst[nvect]); - break; - } - } - } - for (int nvect = 0; nvect < num_interesting_nvect; nvect++) - { - t0 += t0IdxEst[nvect]; - } - t0 /= (float)num_interesting_nvect; - t0Int = (int)(t0+0.5f); -// printf("%s(%d): t0 %.1f --> %d\n",__FUNCTION__,num_interesting_nvect,t0,t0Int); - - - if(t0Int < 0) - t0Int = 0; - if(t0Int >= npts) - t0Int = npts-1; - // second, clear the average trace up to t0. - for(int pt=1;pt highV.A[k]); -#endif + tmpV.V -= rowNoiseV; + +// tmpV.V = LD_VEC8F(mCorr_sigs[r*npts+pt]); //DEBUG - // gain correct -// tmpV.V *= *lgcV; meanV.V += tmpV.V; //sum all wells for mean trace *tPtrV = tmpV.V; itrc += VEC8_SIZE; @@ -1169,6 +841,15 @@ int AdvCompr::PopulateTraceBlock_ComputeMean() mean_trc[pt] = meanPt/(float)ntrcs; } + // copy the second point to the first + mean_trc[0] = mean_trc[1]; + v8f *rtrcV0 = (v8f *) &TRCS_ACCESS(0,0); + v8f *rtrcV1 = (v8f *) &TRCS_ACCESS(0,1); + + for(int idx=0;idx= 0 &&*/ ++good_count == sample_rate) { *sample_col_begin++ = *col_start; -// good_count = 0; check_count++; if(check_count == nSamples) break; @@ -1437,28 +1064,6 @@ void AdvCompr::CreateSampleMatrix() timing.createSample += AdvCTimer() - start; } -void AdvCompr::ComputeEmphasisVector(float *gv, float mult, float adder, float width) -{ -// float dt; -// const float width=10.0f; - float gvssq = 0.0f; - int t0estLocal=t0estIdx+7;// needs to point to the middle of the incorporation - - for (int i=0;i < npts;i++) - { - float dt=i-t0estLocal; - gv[i]=mult*exp(-dt*dt/width)+adder; -// ptgv[i]=COEFF_ACCESS(i,nvect)*gv[i]; - gvssq += gv[i]*gv[i]; - } - gvssq = sqrt(gvssq); - - for (int i=0;i < npts;i++) -// gv[i]/=gvssq; - gv[i] = 1.0f; -} - - float AdvCompr::ExtractVectors() { @@ -1471,8 +1076,6 @@ float AdvCompr::ExtractVectors() for (pt = 0; pt < npts; pt++) gv[pt] = 1.0f; -// ComputeEmphasisVector(gv, 2.0f, 1.4f, 10.0f); - for (int nvect = 0; nvect < hdr.nBasisVec; nvect++) { float ssum = 0; @@ -1544,7 +1147,7 @@ int AdvCompr::GetBitsNeeded() { for (i = 0; i < ntrcs; i++) { - if (trcs_state[i]) + if (trcs_state[i]==0) continue; if (TRC_COEFF_ACC(nv,i) > maxVals[nv]) maxVals[nv] = TRC_COEFF_ACC(nv,i); @@ -1679,7 +1282,7 @@ uint64_t AdvCompr::PackBits(uint64_t *buffer, uint64_t *bufferEnd) { nlvec = nvec; - if (trcs_state[itrc] == 0) + if (trcs_state[itrc]) { for (nv = 0; nv < nlvec; nv++) { @@ -1854,7 +1457,7 @@ int AdvCompr::WriteTraceBlock() // write out the bits needed per vec Write(fd, bitsNeeded, sizeof(bitsNeeded[0]) * nvect); - if(frameRate != 15){ + if(frameRate != 15 && timeTransform){ float nMeanTrc[npts_newfr]; TimeTransform_trace(npts,npts_newfr,timestamps_compr,timestamps_newfr,mean_trc,nMeanTrc,1); @@ -1864,14 +1467,6 @@ int AdvCompr::WriteTraceBlock() float nBasisVects[nvect*npts_newfr]; TimeTransform_trace(npts,npts_newfr,timestamps_compr,timestamps_newfr,basis_vectors,nBasisVects,nvect); -// printf("new basis_vectors:\n"); -// for(int bv=0;bv high || fptr[idx] < low){ + if(mMask[idx]){ + pinned++; + mMask[idx] = 0; + } + } + } + } +// printf("adv: pinned: %d\n",pinned); + +// for(int y=0;y0) { + if (y-span > 0) { + nn_sum[frame] -= SIGS_ACC(start_y-1, frame); + } + if (y+span <= h) { + nn_sum[frame] += SIGS_ACC(end_y, frame); + } + } + nn_avg = nn_sum[frame]; + nn_avg /= (float)(end_y-start_y); + float tmp = SIGS_ACC(y,frame) - nn_avg; + if(tmp != tmp) + tmp=0; + NOISE_ACC(y,frame) = tmp; + + } + } + +#if 0 + // apply the correction right here.. + v8s *srcPtr = (v8s *)&raw[0]; + int frameStrideV=(h*w/8); + + for (int y = 0; y < h; y++) { + for(int x=0;x= 0 && ThreadNum < ADVC_MAX_REGIONS)) diff --git a/Analysis/Image/ChipIdDecoder.cpp b/Analysis/Image/ChipIdDecoder.cpp index 2c1e409b..114c216a 100644 --- a/Analysis/Image/ChipIdDecoder.cpp +++ b/Analysis/Image/ChipIdDecoder.cpp @@ -22,6 +22,7 @@ ChipIdDecodeArrayType ChipIdDecoder::chip_id_str_lookup_array[] = { "p2.2.2", ChipId2_2_2 }, { "900", ChipId_old_P1 }, //for backward compatibility and basecalling of old old chips { "p1.0.20", ChipId1_0_20 }, + { "560", ChipId560 }, { "550", ChipId550 }, { "540", ChipId540 }, { "530", ChipId530 }, @@ -93,6 +94,7 @@ bool ChipIdDecoder::IsProtonChip(){ case ChipId1_0_19: case ChipId1_0_20: case ChipId_old_P1: + case ChipId560: case ChipId550: case ChipId540: case ChipId530: @@ -150,8 +152,8 @@ bool ChipIdDecoder::IsPtwo(){ case ChipId1_2_18: case ChipId2_2_1: case ChipId2_2_2: - case ChipId550: case ChipId551: + case ChipId560: return true; break; default: @@ -213,6 +215,7 @@ bool ChipIdDecoder::BigEnoughForGPU(){ case ChipId2_2_1: case ChipId2_2_2: case ChipId_old_P1: + case ChipId560: case ChipId550: case ChipId540: case ChipId530: diff --git a/Analysis/Image/ChipIdDecoder.h b/Analysis/Image/ChipIdDecoder.h index aafab860..df8a5958 100644 --- a/Analysis/Image/ChipIdDecoder.h +++ b/Analysis/Image/ChipIdDecoder.h @@ -30,7 +30,8 @@ typedef enum ChipId2_1_1 = 21, ChipId2_3_1 = 22, ChipId1_1_541 = 23, - ChipId522 = 24 + ChipId522 = 24, + ChipId560 = 25 } ChipIdEnum; typedef struct diff --git a/Analysis/Image/CorrNoiseCorrector.cpp b/Analysis/Image/CorrNoiseCorrector.cpp index e12d4c6f..8ec118dc 100644 --- a/Analysis/Image/CorrNoiseCorrector.cpp +++ b/Analysis/Image/CorrNoiseCorrector.cpp @@ -78,7 +78,7 @@ double CorrNoiseCorrector::CorrectCorrNoise(RawImage *raw, int correctRows, int double CorrNoiseCorrector::CorrectCorrNoise(short *_image, int _rows, int _cols, int _frames, int correctRows, - int _thumbnail, bool override, bool verbose, int threadNum, int avg) + int _thumbnail, bool override, bool verbose, int threadNum, int avg, int frame_mult) { char *allocPtr; double rc=0; @@ -102,6 +102,8 @@ double CorrNoiseCorrector::CorrectCorrNoise(short *_image, int _rows, int _cols, rows=_rows; cols=_cols; frames=_frames; + if(frame_mult) + fmult=frame_mult; mCorr_mask_len = rows*cols*2; mCorr_mask=(short int *)memalign(64,mCorr_mask_len); @@ -350,7 +352,7 @@ void CorrNoiseCorrector::DebugSaveRowNoise(int correctRows) // make sure our applied correction won't pin any currently un-pinned pixels void CorrNoiseCorrector::FixouterPixels() { - int frameStride=rows*cols; + int frameStride=rows*cols*fmult; int endFrmOffset = (frames-1)*frameStride; for (int idx = 0; idx < rows*cols; idx++){ short int *srcPtr = (short int *) (&image[idx]); @@ -403,7 +405,7 @@ void CorrNoiseCorrector::FixouterPixels() void CorrNoiseCorrector::ReZeroPinnedPixels_cpFirstFrame() { - int frameStride=rows*cols; + int frameStride=rows*cols*fmult; #ifdef SCALE_TRACES int64_t avg_height=0; int64_t avg_cnt=0; @@ -497,8 +499,8 @@ void CorrNoiseCorrector::ReZeroPinnedPixels_cpFirstFrame() double CorrNoiseCorrector::ApplyCorrection_rows() { int y; - int frameStride=rows*cols; - int frameStrideV=rows*cols/8; + int frameStride=rows*cols*fmult; + int frameStrideV=(rows*cols/8)*fmult; double startTime = CNCTimer(); double TotalAvgNoiseSq=0; double TotalAvgNoiseSum=0; @@ -574,7 +576,7 @@ void CorrNoiseCorrector::ApplyCorrection_cols() { for(int y=0 ;y< rows; y++){ v8f_u *corr=(v8f_u *)&NOISE_ACC(0,0,frame); - v8s *srcPtr=(v8s *)&image[frame*rows*cols + y*cols]; + v8s *srcPtr=(v8s *)&image[frame*fmult*rows*cols + y*cols]; for(int x=0 ;x< cols; x+=8,corr++,srcPtr++){ v8s_u corrS; CVT_VEC8F_VEC8S(corrS,(*corr)); @@ -622,7 +624,7 @@ void CorrNoiseCorrector::SumRows() for (frame = 0; frame < frames; frame++) { for (y = 0; y < rows; y++){ - short int *sptr = (short int *) (&image[frame * cols*rows + y * cols]); + short int *sptr = (short int *) (&image[frame * fmult * cols*rows + y * cols]); v8s *maskSumPtr=(v8s *)&mCorr_mask[y*cols]; for(int reg=0;regtimestamps[0]); - if(!headerOnly && timeTransform && rc != 0 && raw->timestamps[0] > 0 && (raw->timestamps[0] < 60 || raw->timestamps[0] > 72)) - { - // more than 10% off from 15fps - // use PCA to time-transform this file to 15fps - AdvComprTest(rawFileName,this,(char *)"7"); // 7 is write a file that's pca compressed and time transformed - free(raw->timestamps); - raw->timestamps=NULL; - free(raw->image); - raw->image=NULL; - raw->rows=saved_rows; - raw->cols=saved_cols; - raw->frames=saved_frames; - raw->uncompFrames=saved_uncompframes; - raw->imageState = saved_ImageState; - char newFname[2048]; - strcpy(newFname,rawFileName); - char *ptr = strstr(newFname,datPostfix); - if(ptr) - sprintf(ptr,"_testPCA.%s", datPostfix); - rc = deInterlace_c ( ( char * ) newFname,&raw->image,&raw->timestamps, - &raw->rows,&raw->cols,&raw->frames,&raw->uncompFrames, - 0,0, - ImageCropping::chipSubRegion.col,ImageCropping::chipSubRegion.row, - ImageCropping::chipSubRegion.col+ImageCropping::chipSubRegion.w,ImageCropping::chipSubRegion.row+ImageCropping::chipSubRegion.h, - ignoreChecksumErrors, &raw->imageState ); - } +// if(!headerOnly && timeTransform && rc != 0 && raw->timestamps[0] > 0 && (raw->timestamps[0] < 60 || raw->timestamps[0] > 72)) +// { +// // more than 10% off from 15fps +// // use PCA to time-transform this file to 15fps +// AdvComprTest(rawFileName,this,(char *)"7"); // 7 is write a file that's pca compressed and time transformed +// free(raw->timestamps); +// raw->timestamps=NULL; +// free(raw->image); +// raw->image=NULL; +// raw->rows=saved_rows; +// raw->cols=saved_cols; +// raw->frames=saved_frames; +// raw->uncompFrames=saved_uncompframes; +// raw->imageState = saved_ImageState; +// char newFname[2048]; +// strcpy(newFname,rawFileName); +// char *ptr = strstr(newFname,datPostfix); +// if(ptr) +// sprintf(ptr,"_testPCA.%s", datPostfix); +// rc = deInterlace_c ( ( char * ) newFname,&raw->image,&raw->timestamps, +// &raw->rows,&raw->cols,&raw->frames,&raw->uncompFrames, +// 0,0, +// ImageCropping::chipSubRegion.col,ImageCropping::chipSubRegion.row, +// ImageCropping::chipSubRegion.col+ImageCropping::chipSubRegion.w,ImageCropping::chipSubRegion.row+ImageCropping::chipSubRegion.h, +// ignoreChecksumErrors, &raw->imageState ); +// } if ( ImageCropping::chipSubRegion.h != 0 ) diff --git a/Analysis/Image/LSRowImageProcessor.cpp b/Analysis/Image/LSRowImageProcessor.cpp index ae4db104..15e3efae 100644 --- a/Analysis/Image/LSRowImageProcessor.cpp +++ b/Analysis/Image/LSRowImageProcessor.cpp @@ -246,7 +246,7 @@ bool LSRowImageProcessor::GenerateGroupCorrection(int group_num,float *vect_outp // make sure derived coefficients are valid for(int row=0;row < nLen;row++) - if(isnan(coeffs(row))) + if(std::isnan(coeffs(row))) { result_ok = false; break; diff --git a/Analysis/Image/PCACompression.cpp b/Analysis/Image/PCACompression.cpp index a6619f95..5e68e0a6 100755 --- a/Analysis/Image/PCACompression.cpp +++ b/Analysis/Image/PCACompression.cpp @@ -32,6 +32,8 @@ int PCACompr::Compress() else ComputeOrderVect(nvect, order++); + smoothNextVector(nvect,1); + EnsureOrthogonal(nvect, (nvect < nRvect)); SubtractVector(nvect, 1); @@ -50,6 +52,28 @@ int PCACompr::Compress() return(total_iter); } +void PCACompr::smoothNextVector(int nvect, int blur) +{ + float trc[npts]; + + for (int j=0;j < npts;j++) + trc[j] = COEFF_ACCESS(j,nvect); + + for (int j=0;j < npts;j++){ + float sum=0.0f; + int start_frame = std::max(0,j-blur); + int end_frame = std::min(npts,j+blur+1); + for(int fr=start_frame;fr end_threshold) && (iter < 10)) && (!failed)); + while (((fabs(tmag - last_tmag)/tmag > end_threshold) && (iter < 20)) && (!failed)); if (failed) { diff --git a/Analysis/Image/PCACompression.h b/Analysis/Image/PCACompression.h index e72b1dfd..2072d627 100755 --- a/Analysis/Image/PCACompression.h +++ b/Analysis/Image/PCACompression.h @@ -30,6 +30,7 @@ class PCACompr{ void EnsureOrthogonal(int nvect, int check); int ComputeNextVector(int nvect, int skip); void ComputeEmphasisVector(float *gv, float mult, float adder, float width); + void smoothNextVector(int nvect, int blur); int nRvect; diff --git a/Analysis/Image/Vecs.h b/Analysis/Image/Vecs.h index df2133d0..f9198c4e 100644 --- a/Analysis/Image/Vecs.h +++ b/Analysis/Image/Vecs.h @@ -16,7 +16,9 @@ #define VEC2_SIZE 2 #define VEC4_SIZE 4 #define VEC8_SIZE 8 +#define VEC16_SIZE 16 #define VEC32_SIZE 32 +#define VEC64_SIZE 64 #define VEC4F_SIZE_B (VEC4_SIZE*4) #define VEC4I_SIZE_B (VEC4_SIZE*4) @@ -27,8 +29,12 @@ #define VEC8F_SIZE_B (VEC8_SIZE*4) #define VEC8I_SIZE_B (VEC8_SIZE*4) #define VEC8S_SIZE_B (VEC8_SIZE*2) - +#define VEC16S_SIZE_B (VEC16_SIZE*2) +#define VEC16F_SIZE_B (VEC16_SIZE*4) +#define VEC32S_SIZE_B (VEC32_SIZE*2) #define VEC32F_SIZE_B (VEC32_SIZE*4) +#define VEC64S_SIZE_B (VEC64_SIZE*2) +#define VEC64F_SIZE_B (VEC64_SIZE*4) #define LD_VEC4F(x) (v4f) {x,x,x,x} #define LD_VEC4I(x) (v4i) {x,x,x,x} @@ -39,7 +45,6 @@ #define LD_VEC8S(x) (v8s) {x,x,x,x,x,x,x,x} #define LD_VEC8SU(x) (v8su) {x,x,x,x,x,x,x,x} -#define LD_VEC32F(x) (v32f) {x,x,x,x,x,x,x,x,x,x,x,x,x,x,x,x,x,x,x,x,x,x,x,x,x,x,x,x,x,x,x,x}; #ifdef WIN32 //typedef struct{ @@ -51,6 +56,16 @@ typedef int v4i __attribute__ ((vector_size (VEC4I_SIZE_B))); typedef short int v4s __attribute__ ((vector_size (VEC4S_SIZE_B))); typedef long long int v4di __attribute__ ((vector_size (VEC4DI_SIZE_B))); typedef long long int v2di __attribute__ ((vector_size (VEC2DI_SIZE_B))); +typedef float v8f __attribute__ ((vector_size (VEC8F_SIZE_B))); +typedef float v16f __attribute__ ((vector_size (VEC16F_SIZE_B))); +typedef int v8i __attribute__ ((vector_size (VEC8I_SIZE_B))); +typedef short int v8s __attribute__ ((vector_size (VEC8S_SIZE_B))); +typedef short unsigned int v8su __attribute__ ((vector_size (VEC8S_SIZE_B))); +typedef short int v16s __attribute__ ((vector_size (VEC16S_SIZE_B))); +typedef short int v32s __attribute__ ((vector_size (VEC32S_SIZE_B))); +typedef float v32f __attribute__ ((vector_size (VEC32F_SIZE_B))); +typedef short int v64s __attribute__ ((vector_size (VEC64S_SIZE_B))); +typedef float v64f __attribute__ ((vector_size (VEC64F_SIZE_B))); typedef union{ v4f V; @@ -66,42 +81,64 @@ typedef union{ short int A[VEC4_SIZE]; }v4s_u; - - -typedef float v8f __attribute__ ((vector_size (VEC8F_SIZE_B))); -typedef int v8i __attribute__ ((vector_size (VEC8I_SIZE_B))); -typedef short int v8s __attribute__ ((vector_size (VEC8S_SIZE_B))); -typedef short unsigned int v8su __attribute__ ((vector_size (VEC8S_SIZE_B))); -typedef float v32f __attribute__ ((vector_size (VEC32F_SIZE_B))); - -typedef union{ - v8f V; - float A[VEC8_SIZE]; -}v8f_u; typedef union{ v8i V; int A[VEC8_SIZE]; }v8i_u; typedef union{ v8s V; + v8s V8[1]; short int A[VEC8_SIZE]; }v8s_u; +typedef union{ + v8f V; + v8f V8[1]; + float A[VEC8_SIZE]; +}v8f_u; typedef union{ v8su V; short unsigned int A[VEC8_SIZE]; }v8su_u; +typedef union{ + v16s V; + v8s V8[2]; + short int A[VEC16_SIZE]; +}v16s_u; +typedef union{ + v16f V; + v8f V8[2]; + float A[VEC16_SIZE]; +}v16f_u; + +typedef union{ + v32s V; + v8s V8[4]; + short int A[VEC32_SIZE]; +}v32s_u; typedef union{ v32f V; + v8f V8[4]; float A[VEC32_SIZE]; }v32f_u; +typedef union{ + v64s V; + v8s V8[8]; + short int A[VEC64_SIZE]; +}v64s_u; +typedef union{ + v64f V; + v8f V8[8]; + float A[VEC64_SIZE]; +}v64f_u; -#ifdef __AVX__ + +#if 0 //def __AVX__ // Clang: __builtin_ia32_pshufd has been removed (Jun 2009) (Use _mm_shuffle_epi32 directly) // http://llvm.org/viewvc/llvm-project?view=revision&revision=72995 // http://llvm.org/viewvc/llvm-project?view=revision&revision=72996 -#define LD_VEC8S_CVT_VEC8F(src_ptr,output_var) {\ +#define LD8_VS_VFI(src_ptr,output_var) {\ /* convert the 2 4(16-bit ints) to 2 4(32-bit ints) samples*/ \ register v4i srcData=*((v4i*)src_ptr); \ register v4i LtmpL = __builtin_ia32_pmovsxwd128 ((v8s)srcData); \ @@ -112,16 +149,25 @@ typedef union{ LvalV = __builtin_ia32_vinsertf128_si256(LvalV,LtmpL,0); \ LvalV = __builtin_ia32_vinsertf128_si256(LvalV,LtmpH,1); \ /* then, convert the 8(32-bit ints) to 8(32-bit floats) */ \ - (output_var).V = __builtin_ia32_cvtdq2ps256(LvalV); \ + (output_var) = __builtin_ia32_cvtdq2ps256(LvalV); \ } + #else -#define LD_VEC8S_CVT_VEC8F(src_ptr,output_var) {\ +#define LD8_VS_VFI(src_ptr,output_var) {\ for(int k_idx=0;k_idx #include "LinuxCompat.h" #include - +#include +#include +#include Mask::Mask ( int _w, int _h ) { @@ -57,11 +59,6 @@ Mask::Mask ( const char *fileName, bool fillMask ) fclose ( in ); } -Mask::~Mask() -{ - mask.clear(); -} - void Mask::Init ( int _w, int _h ) { Init ( _w,_h,MaskEmpty ); @@ -156,12 +153,12 @@ void Mask::Set ( int x, int y, MaskType type ) } } -// adds specified type flag to all wells in the mask +// sets specified type flag to all wells in the mask; resets all other flags void Mask::SetAll(MaskType type ) { int i; for ( i=0;i 0) { + sscanf(line.c_str(), "%d\t%d", &maskStart, &maskEnd); + + if( y>=offset_y && y=offset_x ){ //fill in the beginning of the row + for( int x = 0; x < std::min(maskStart-offset_x, size_x); ++x){ + mask[x+((y-offset_y)*this->w)] = MaskExclude; + masked++; + } + } + + if( maskEnd < (offset_x+size_x) ){ //fill in the end of the row + //note that maskEnd==0 of the whole row is excluded + for( int x = std::max(0, maskEnd - offset_x); xw)] = MaskExclude; + masked++; + } + } + } + } + } + std::cerr << "Total Masked Wells: " << masked << "\n"; + return 0; +} + int Mask::DumpStats ( Region region, char *fileName, bool showWashouts ) const { int x; diff --git a/Analysis/Mask/Mask.h b/Analysis/Mask/Mask.h index 499d0099..48de3abd 100644 --- a/Analysis/Mask/Mask.h +++ b/Analysis/Mask/Mask.h @@ -50,7 +50,7 @@ class Mask Mask ( int w, int h ); Mask ( Mask *origmask ); Mask ( const char *fileName, bool fillMask=true ); - virtual ~Mask(); + virtual ~Mask(){} Mask() { mask.clear(); } @@ -118,6 +118,8 @@ class Mask int MaskList ( char *fileName, MaskType these, Region region ) const; int WriteRaw ( const char *fileName ) const; int SetMask ( const char *fileName ); + int SetMaskFullChipText(const char *fileName, int offset_x, int offset_y, int size_x, int size_y); + void LoadMaskAndAbortOnFailure(const char *maskFileName); void UpdateBeadFindOutcomes( Region &wholeChip, char const *maskFileName, bool not_single_beadfind, int update_stats, char const *maskStatsName); int DumpStats ( Region region, char *fileName, bool showWashouts = true ) const; @@ -137,7 +139,8 @@ class Mask // void OnlySomeWells(std::vector mWellIdx); std::vector mask; - protected: + +protected: int32_t w, h; int32_t xOffset, yOffset; //uint16_t *mask; diff --git a/Analysis/Mask/PinnedInFlow.cpp b/Analysis/Mask/PinnedInFlow.cpp index 3f985855..311a271d 100644 --- a/Analysis/Mask/PinnedInFlow.cpp +++ b/Analysis/Mask/PinnedInFlow.cpp @@ -233,60 +233,109 @@ int PinnedInFlow::Update (int flow, Image *img, float *gainPtr) } #ifdef __AVX__ - if ((cols % VEC8_SIZE) == 0 && gainPtr != NULL) - { + if ((cols % VEC8_SIZE) == 0) { int k, idx; int frameStride = rows * cols; short int *src; +#define MY_VEC_SIZE 8 +#define MY_VECF v8f_u + v8f_u highV, lowV; - v8f_u tmpV; - v8f *gainPtrV; - v8f_u pinnedV; + v8f_u dummy8 = { }; + MY_VECF tmpV; + MY_VECF *gainPtrV; + MY_VECF pinnedV; + MY_VECF dummy = { }; short int *sptr; - highV.V = LD_VEC8F((const float) pinHigh); - lowV.V = LD_VEC8F((const float)pinLow); + highV.V = dummy8.V + (float) pinHigh; + lowV.V = dummy8.V + (float) pinLow; src = (short int *) (raw->image); - gainPtrV=(v8f *)gainPtr; - for(idx=0;idxV = tmpS.V; + } + gainPtrV++; + // if any of the 8 pixels are pinned + int somePinned = 0; + for (uint l = 0; l < sizeof(pinnedV.V8) / sizeof(pinnedV.V8[0]); + l++) { + if (__builtin_ia32_ptestnzc256((v4di) pinnedV.V8[l], + (v4di ) { -1, -1, -1, -1 })) + somePinned = 1; + } - CVT_VEC8F_VEC8S((*(v8s_u *)sptr),tmpV); + if (somePinned) { + for (k = 0; k < MY_VEC_SIZE; k++) { + if (pinnedV.A[k]) + SetPinned(idx + k, flow); + } + } } - gainPtrV++; - // if any of the 8 pixels are pinned - if(__builtin_ia32_ptestnzc256((v4di)pinnedV.V,(v4di){-1,-1,-1,-1}) ) - { - for (k = 0; k < VEC8_SIZE; k++) - { - if(pinnedV.A[k]) - SetPinned(idx+k, flow); + } else { + // no gain correction + for (idx = 0; idx < frameStride; idx += MY_VEC_SIZE, src += + MY_VEC_SIZE) { + pinnedV.V = dummy.V + 0; + + for (frame = 0; frame < frames; frame++) { + sptr = &src[frame * frameStride]; + + LD_VS_VF(sptr, tmpV); + + // now, do the pinned pixel comparisons + for (uint l = 0; + l < sizeof(pinnedV.V8) / sizeof(pinnedV.V8[0]); + l++) { + pinnedV.V8[l] += __builtin_ia32_cmpps256(tmpV.V8[l], + lowV.V, _CMP_LT_OS); + pinnedV.V8[l] += __builtin_ia32_cmpps256(highV.V, + tmpV.V8[l], _CMP_LT_OS); + } + } + // if any of the 8 pixels are pinned + int somePinned = 0; + for (uint l = 0; l < sizeof(pinnedV.V8) / sizeof(pinnedV.V8[0]); + l++) { + if (__builtin_ia32_ptestnzc256((v4di) pinnedV.V8[l], + (v4di ) { -1, -1, -1, -1 })) + somePinned = 1; + } + + if (somePinned) { + for (k = 0; k < MY_VEC_SIZE; k++) { + if (pinnedV.A[k]) + SetPinned(idx + k, flow); + } } } - } - } - else + } + }else #endif { int16_t *pixPtr; diff --git a/Analysis/Separator/BeadfindStats.cpp b/Analysis/Separator/BeadfindStats.cpp index 9f469742..ae967d7f 100644 --- a/Analysis/Separator/BeadfindStats.cpp +++ b/Analysis/Separator/BeadfindStats.cpp @@ -37,10 +37,11 @@ void CalcBfT0(DifSepOpt &opts, std::vector &t0vec, Mask &mask, int gridMo img.FilterForPinned(&mask, MaskEmpty, false); T0Calc t0; t0.SetWindowSize(3); - t0.SetMinFirstHingeSlope(-5.0/raw->baseFrameRate); - t0.SetMaxFirstHingeSlope(300.0/raw->baseFrameRate); - t0.SetMinSecondHingeSlope(-20000.0/raw->baseFrameRate); - t0.SetMaxSecondHingeSlope(-10.0/raw->baseFrameRate); + t0.SetMinFirstHingeSlope(opts.beadfindBfThreshold[0]/raw->baseFrameRate); + t0.SetMaxFirstHingeSlope(opts.beadfindBfThreshold[1]/raw->baseFrameRate); + t0.SetMinSecondHingeSlope(opts.beadfindBfThreshold[2]/raw->baseFrameRate); + t0.SetMaxSecondHingeSlope(opts.beadfindBfThreshold[3]/raw->baseFrameRate); + t0.SetDebugLevel(opts.outputDebug); short *data = raw->image; int frames = raw->frames; t0.SetMask(&mask); @@ -112,6 +113,10 @@ int main(int argc, const char *argv[]) { opts.t0MeshStepX = size; opts.t0MeshStepY = size; } + + opts.beadfindBfThreshold = std::vector({-5, 300, -20000, -10}); + + Mask mask; int row = 0, col = 0; std::vector t0; @@ -130,7 +135,7 @@ int main(int argc, const char *argv[]) { } } - SetExcludeMask(loc_context, &mask, ChipIdDecoder::GetChipType(), mask.H(), mask.W()); + SetExcludeMask(loc_context, &mask, ChipIdDecoder::GetChipType(), mask.H(), mask.W(), std::string(""), false); size_t numWells = mask.H() * mask.W(); int excludeWells = 0; for (size_t i = 0; i < numWells; i++) { diff --git a/Analysis/Separator/DifferentialSeparator.cpp b/Analysis/Separator/DifferentialSeparator.cpp index 7345f796..6c70ccd1 100644 --- a/Analysis/Separator/DifferentialSeparator.cpp +++ b/Analysis/Separator/DifferentialSeparator.cpp @@ -1347,11 +1347,13 @@ void DifferentialSeparator::CalcBfT0(DifSepOpt &opts, std::vector &t0vec, copy(raw->timestamps, raw->timestamps+raw->frames, &mBFTimePoints[0]); T0Calc t0; t0.SetWindowSize(3); - t0.SetMinFirstHingeSlope(-5.0/raw->baseFrameRate); - t0.SetMaxFirstHingeSlope(300.0/raw->baseFrameRate); - t0.SetMinSecondHingeSlope(-20000.0/raw->baseFrameRate); - t0.SetMaxSecondHingeSlope(-10.0/raw->baseFrameRate); + t0.SetMinFirstHingeSlope(opts.beadfindBfThreshold[0]/raw->baseFrameRate); + t0.SetMaxFirstHingeSlope(opts.beadfindBfThreshold[1]/raw->baseFrameRate); + t0.SetMinSecondHingeSlope(opts.beadfindBfThreshold[2]/raw->baseFrameRate); + t0.SetMaxSecondHingeSlope(opts.beadfindBfThreshold[3]/raw->baseFrameRate); t0.SetBadWells(&mFilteredWells[0]); + t0.SetDebugLevel(opts.outputDebug); + short *data = raw->image; int frames = raw->frames; t0.SetMask(&mMask); @@ -1420,11 +1422,13 @@ void DifferentialSeparator::CalcAcqT0(DifSepOpt &opts, std::vector &t0vec copy(raw->timestamps, raw->timestamps+raw->frames, &mBFTimePoints[0]); T0Calc t0; t0.SetWindowSize(4); - t0.SetMinFirstHingeSlope(-10.0/raw->baseFrameRate); - t0.SetMaxFirstHingeSlope(3.0/raw->baseFrameRate); - t0.SetMinSecondHingeSlope(5.0/raw->baseFrameRate); - t0.SetMaxSecondHingeSlope(500.0/raw->baseFrameRate); + t0.SetMinFirstHingeSlope(opts.beadfindAcqThreshold[0]/raw->baseFrameRate); + t0.SetMaxFirstHingeSlope(opts.beadfindAcqThreshold[1]/raw->baseFrameRate); + t0.SetMinSecondHingeSlope(opts.beadfindAcqThreshold[2]/raw->baseFrameRate); + t0.SetMaxSecondHingeSlope(opts.beadfindAcqThreshold[3]/raw->baseFrameRate); t0.SetBadWells(&mFilteredWells[0]); + t0.SetDebugLevel(opts.outputDebug); + short *data = raw->image; int frames = raw->frames; t0.SetMask(&mMask); @@ -1487,11 +1491,13 @@ void DifferentialSeparator::CalcAcqT0(DifSepOpt &opts, std::vector &t0vec copy(raw->timestamps, raw->timestamps+raw->frames, &mBFTimePoints[0]); T0Calc t0; t0.SetWindowSize(4); - t0.SetMinFirstHingeSlope(-10.0/raw->baseFrameRate); - t0.SetMaxFirstHingeSlope(3.0/raw->baseFrameRate); - t0.SetMinSecondHingeSlope(5.0/raw->baseFrameRate); - t0.SetMaxSecondHingeSlope(500.0/raw->baseFrameRate); + t0.SetMinFirstHingeSlope(opts.beadfindAcqThreshold[0]/raw->baseFrameRate); + t0.SetMaxFirstHingeSlope(opts.beadfindAcqThreshold[1]/raw->baseFrameRate); + t0.SetMinSecondHingeSlope(opts.beadfindAcqThreshold[2]/raw->baseFrameRate); + t0.SetMaxSecondHingeSlope(opts.beadfindAcqThreshold[3]/raw->baseFrameRate); t0.SetBadWells(&mFilteredWells[0]); + t0.SetDebugLevel(opts.outputDebug); + short *data = raw->image; int frames = raw->frames; t0.SetMask(&mMask); diff --git a/Analysis/Separator/DifferentialSeparator.h b/Analysis/Separator/DifferentialSeparator.h index e031ad81..c5dad178 100644 --- a/Analysis/Separator/DifferentialSeparator.h +++ b/Analysis/Separator/DifferentialSeparator.h @@ -162,6 +162,8 @@ class DifSepOpt bool col_pair_pixel_xtalk_correct; float pair_xtalk_fraction; bool corr_noise_correct; + std::vector beadfindAcqThreshold; + std::vector beadfindBfThreshold; }; /** diff --git a/Analysis/Separator/T0Model.h b/Analysis/Separator/T0Model.h index b9e51e01..ad2a079f 100644 --- a/Analysis/Separator/T0Model.h +++ b/Analysis/Separator/T0Model.h @@ -338,7 +338,7 @@ class T0Finder { } /** Look at trace data provided by Y and find when nuc hits the wells */ - bool FindT0Time(float *Y, float *X, size_t size) { + bool FindT0Time(float *Y, float *X, size_t size, FILE* fdbg) { mX.resize(size); for (size_t i = 0; i < mX.size(); i++) { mX[i] = X[i]; @@ -356,6 +356,13 @@ class T0Finder { t0.SetFirstRange(i - mWindowSize, i); t0.SetSecondRange(i+1, i + mWindowSize + 1); t0.FitModel(Y, &mX[0]); + if( fdbg ){ + bool firstOk = (t0.mHingeModel.GetFirst().GetSlope() >= mFirstSlopeRange[0] && + t0.mHingeModel.GetFirst().GetSlope() <= mFirstSlopeRange[1]); + bool secondOk = (t0.mHingeModel.GetSecond().GetSlope() >= mSecondSlopeRange[0] && + t0.mHingeModel.GetSecond().GetSlope() <= mSecondSlopeRange[1]); + fprintf(fdbg, "%g %g %g %g %g %d %d\n", t0.GetT0Est()/66., 66.*t0.mHingeModel.GetFirst().GetSlope(), 66.*t0.mHingeModel.GetSecond().GetSlope(), t0.GetSsqDiff(), t0.GetSsqRatio(), firstOk, secondOk ); + } if (first) { mBestT0 = t0; maxSsqDiff = 0.0; @@ -371,6 +378,8 @@ class T0Finder { } } } + if( fdbg ) + fprintf(fdbg, "%g %g %g %g %g\n", mBestT0.GetT0Est()/66., 66.*mBestT0.mHingeModel.GetFirst().GetSlope(), 66.*mBestT0.mHingeModel.GetSecond().GetSlope(), mBestT0.GetSsqDiff(), mBestT0.GetSsqRatio() ); return found; } diff --git a/Analysis/T0Calc.h b/Analysis/T0Calc.h index a7d1ef41..4a17d321 100644 --- a/Analysis/T0Calc.h +++ b/Analysis/T0Calc.h @@ -63,7 +63,7 @@ class T0Calc { void SetMinFirstHingeSlope(float minSlope) { mMinFirstHingeSlope = minSlope; } void SetMaxSecondHingeSlope(float maxSlope) { mMaxSecondHingeSlope = maxSlope; } void SetMinSecondHingeSlope(float minSlope) { mMinSecondHingeSlope = minSlope; } - + void SetDebugLevel( int debugLevel ) { outputDebug = debugLevel; } void SetMask(Mask *mask) { mMask = mask; } /** Setup our t0 calculator. */ @@ -102,7 +102,7 @@ class T0Calc { /** Algorithm to fit t0 for this trace using a two piece linear model. */ static void CalcT0(T0Finder &finder, std::vector &trace, std::vector ×tamps, - T0Prior &prior, float &t0) { + T0Prior &prior, float &t0, FILE* fdbg=0) { /* Convert the t0 prior in time into frames. */ std::vector::iterator tstart; int frameEnd = trace.size(); @@ -118,7 +118,7 @@ class T0Calc { frameStart = frame - 1; } finder.SetSearchRange(frameStart, frameEnd); - bool ok = finder.FindT0Time(&trace[0], ×tamps[0], trace.size()); + bool ok = finder.FindT0Time(&trace[0], ×tamps[0], trace.size(), fdbg); if (ok) { t0 = finder.GetT0Est(); t0 = (prior.mT0Weight * prior.mT0Prior) + t0; @@ -174,18 +174,27 @@ class T0Calc { int tooFew = 0; int enough = 0; for (size_t bIx = 0; bIx < numBin; bIx++) { - std::pair > &bin = mRegionSum.GetItem(bIx); - if (bin.first < 20) { - tooFew++; - continue; - } - enough++; - for (size_t fIx = 0; fIx < bin.second.size(); fIx++) { - bin.second[fIx] = bin.second[fIx] / bin.first; - } - T0Prior &prior = mT0Prior.GetItem(bIx); - float &t0 = mT0.GetItem(bIx); - T0Calc::CalcT0(mFinder, bin.second, mTimeStamps, prior, t0); + FILE* fdbg = 0; + if( outputDebug>4){ + char fname[1024]; + sprintf(fname,"bin_%d.dbg",(int)bIx); + FILE* fdbg = fopen(fname,"w"); + } + std::pair > &bin = mRegionSum.GetItem(bIx); + if (bin.first < 20) { + tooFew++; + continue; + } + enough++; + for (size_t fIx = 0; fIx < bin.second.size(); fIx++) { + bin.second[fIx] = bin.second[fIx] / bin.first; + } + T0Prior &prior = mT0Prior.GetItem(bIx); + float &t0 = mT0.GetItem(bIx); + T0Calc::CalcT0(mFinder, bin.second, mTimeStamps, prior, t0, fdbg); + + if(fdbg) + fclose(fdbg); } // std::cout << "T0 Fitting: " << enough << " enough " << tooFew << " too few good wells." << std::endl; } @@ -677,6 +686,7 @@ class T0Calc { float mMinSdThresh; int mPixHigh; int mPixLow; + int outputDebug; }; diff --git a/Analysis/TMAP/CMakeLists.txt b/Analysis/TMAP/CMakeLists.txt index c98e5dc5..8bd6b509 100644 --- a/Analysis/TMAP/CMakeLists.txt +++ b/Analysis/TMAP/CMakeLists.txt @@ -172,9 +172,19 @@ configure_file ( "${PROJECT_BINARY_DIR}/config.h" @ONLY ) - add_executable(tmap ${tmap_SOURCES}) set_target_properties(tmap PROPERTIES COMPILE_FLAGS "-D_TMAP_BWT_RUN_TYPE=0 -D_USE_KNETFILE -DSAMTOOLS_MAIN -D__STDC_LIMIT_MACROS -DTMAP_MMAP=1") + +include(CheckCCompilerFlag) +check_c_compiler_flag(-fdiagnostics-color=never HAS_DIAGNOSTICS_COLOR) +if (HAS_DIAGNOSTICS_COLOR) + target_compile_options(tmap PUBLIC -fdiagnostics-color=never) +endif() + + + +# + target_link_libraries (tmap m z pthread bz2) install(TARGETS tmap DESTINATION bin) diff --git a/Analysis/TMAP/src/index/tmap_refseq.c b/Analysis/TMAP/src/index/tmap_refseq.c index 7ce210bc..881c8c47 100644 --- a/Analysis/TMAP/src/index/tmap_refseq.c +++ b/Analysis/TMAP/src/index/tmap_refseq.c @@ -1235,7 +1235,7 @@ tmap_refseq_read_bed_core(tmap_refseq_t *refseq, char *bedfile, int flag, char * // ZZ:current ampl is contained in the previous one continue; } else if (b[num-1] == beg) { - // ZZ:current one containing the previous one with the same begin, and larger ending, use the current to replace the previous + // ZZ:current one has same beg, but larger end, replace previous one num--; } } @@ -1265,11 +1265,11 @@ find_next_bed(tmap_refseq_t *refseq, int cur_ind, int cur_pos, int *b, int *e, i { int i = *n; *b = -1; - while (i < refseq->bednum[cur_ind] && refseq->bedend[cur_ind][i] < cur_pos) i++; + while (i < refseq->bednum[cur_ind] && refseq->bedend[cur_ind][i] <= cur_pos) i++; *n = i; if (i < refseq->bednum[cur_ind]) { - *b = refseq->bedstart[cur_ind][i]-1; - *e = refseq->bedend[cur_ind][i]-1; + *b = refseq->bedstart[cur_ind][i]; + *e = refseq->bedend[cur_ind][i]; } } @@ -1364,7 +1364,7 @@ int tmap_refseq_fasta2maskedfasta_main(int argc, char *argv[]) // now cur_chr == chr char *s = line; while (*s != '\n' && *s != 0) { - if(cur_pos > e) { + if(cur_pos >= e) { find_next_bed(refseq, chr_ind, cur_pos, &b, &e, &n); if (b == -1) { all_N(s); diff --git a/Analysis/TMAP/src/io/tmap_file.c b/Analysis/TMAP/src/io/tmap_file.c index e4a73422..d33e3c7c 100644 --- a/Analysis/TMAP/src/io/tmap_file.c +++ b/Analysis/TMAP/src/io/tmap_file.c @@ -495,55 +495,64 @@ int32_t tmap_log_enabled () return logging_enabled; } -int32_t tmap_log (const char* format, ...) +// waits for a logging mutex to get released, then acquires it +void tmap_log_record_begin () { - int32_t n = 0; - va_list ap; - if (!logging_enabled) - return n; #ifdef HAVE_LIBPTHREAD - pthread_mutex_lock (&log_mutex); + pthread_mutex_lock (&log_mutex); #endif - va_start(ap, format); - if (!log_file_pointer) - { - n = vprintf(format, ap); - fflush (stdout); - } - else - { - n = vfprintf(log_file_pointer, format, ap); - fflush (log_file_pointer); - } - va_end(ap); +} + +// releases logging mutex +void tmap_log_record_end () +{ #ifdef HAVE_LIBPTHREAD - pthread_mutex_unlock (&log_mutex); + pthread_mutex_unlock (&log_mutex); #endif - return n; +} + +int32_t tmap_log (const char* format, ...) +{ + int32_t n = 0; + va_list ap; + va_start (ap, format); + n = tmap_vlog (format, ap); + va_end (ap); + return n; } int32_t tmap_vlog (const char* format, va_list ap) { - int32_t n = 0; - if (!logging_enabled) + int32_t n = 0; + if (!logging_enabled) + return n; + if (!log_file_pointer) + { + n = vprintf(format, ap); + fflush (stdout); + } + else + { + n = vfprintf(log_file_pointer, format, ap); + fflush (log_file_pointer); + } return n; -#ifdef HAVE_LIBPTHREAD - pthread_mutex_lock (&log_mutex); -#endif - if (!log_file_pointer) - { - n = vprintf(format, ap); - fflush (stdout); - } - else - { - n = vfprintf(log_file_pointer, format, ap); - fflush (log_file_pointer); - } +} -#ifdef HAVE_LIBPTHREAD - pthread_mutex_unlock (&log_mutex); -#endif - return n; +int32_t tmap_log_s (const char* format, ...) +{ + int32_t n = 0; + va_list ap; + va_start (ap, format); + n = tmap_vlog_s (format, ap); + va_end (ap); + return n; } +int32_t tmap_vlog_s (const char* format, va_list ap) +{ + tmap_log_record_begin (); + int32_t n = tmap_vlog (format, ap); + tmap_log_record_end (); + return n; +} diff --git a/Analysis/TMAP/src/io/tmap_file.h b/Analysis/TMAP/src/io/tmap_file.h index 760098ce..ad3e8d11 100644 --- a/Analysis/TMAP/src/io/tmap_file.h +++ b/Analysis/TMAP/src/io/tmap_file.h @@ -189,8 +189,12 @@ tmap_file_fflush(tmap_file_t *fp, int32_t gz_flush); void tmap_log_enable (FILE* fp); void tmap_log_disable (); int32_t tmap_log_enabled (); +void tmap_log_record_begin (); +void tmap_log_record_end (); int32_t tmap_log (const char* format, ...); int32_t tmap_vlog (const char* format, va_list ap); +int32_t tmap_log_s (const char* format, ...); +int32_t tmap_vlog_s (const char* format, va_list ap); #endif // TMAP_FILE_H diff --git a/Analysis/TMAP/src/map/tmap_map_driver.c b/Analysis/TMAP/src/map/tmap_map_driver.c index 84c3a2b7..271ba543 100644 --- a/Analysis/TMAP/src/map/tmap_map_driver.c +++ b/Analysis/TMAP/src/map/tmap_map_driver.c @@ -8,7 +8,7 @@ #ifdef HAVE_LIBPTHREAD #include #endif -#include +#include #include "../util/tmap_error.h" #include "../util/tmap_alloc.h" #include "../util/tmap_definitions.h" @@ -195,7 +195,7 @@ unsigned tailrep_cont (unsigned pattlen, const char* pattern, unsigned textlen, return rep_beg; } -/* this function finds maximal length of the peridicity suffix in text +/* this function finds maximal length of the periodicity suffix in text returns first position in a text that belongs to such periodicity position */ unsigned tailrep (unsigned textlen, const char* text, unsigned max_period) { @@ -491,169 +491,481 @@ unsigned trim_cigar (uint32_t** cigar_buff, unsigned orig_cigar_sz, unsigned qry return trim_cigar_left (cigar_buff, orig_cigar_sz, qry_len - trim_at, ref_rep_off_p); } -static void cigar_log (const uint32_t* cigar, unsigned cigar_sz) +static uint8_t fully_clipped (uint32_t* cigar, int32_t n_cigar) { - const uint32_t* sent; - for (sent = cigar+cigar_sz; cigar != sent; ++cigar) + // check if there are any matches in cigar + // (less conservative would be a check for any reference consuming op, but since mapping that consumes reference with no match is senseless, we'll consider it no-map as well.) + int32_t idx; + for (idx = 0; idx != n_cigar; ++idx) + if (TMAP_SW_CIGAR_OP (cigar [idx]) == BAM_CMATCH) + return 0; + return 1; +} + +void realign_read (tmap_seq_t* qryseq, tmap_refseq_t* refseq, tmap_map_sams_t* sams, struct RealignProxy* realigner, tmap_map_stats_t *stat, uint32_t log_text_als) +{ + // extract query + const char* qryname = tmap_seq_get_name (qryseq)->s; + tmap_string_t* qrybases = tmap_seq_get_bases (qryseq); + const char* qry = qrybases->s; + unsigned matchidx; + for (matchidx = 0; matchidx < sams->n; ++matchidx) { - uint32_t curop = bam_cigar_op (*cigar); - uint32_t count = bam_cigar_oplen (*cigar); - char schar; - switch (curop) + // extract packed cigar and it's length + tmap_map_sam_t* match = sams->sams + matchidx; + unsigned orig_cigar_sz = match->n_cigar; + uint32_t* orig_cigar = tmap_calloc (orig_cigar_sz, sizeof (uint32_t), "orig_cigar"); + memcpy (orig_cigar, match->cigar, match->n_cigar * sizeof (uint32_t)); + // extract seqid + unsigned ref_id = match->seqid; + // extract offset + unsigned ref_off = match->pos; + // extract read direction in alignment + uint8_t forward = (match->strand == 0) ? 1 : 0; + // make inverse/complement copy of the query if direction is reverse + if (!forward) { - case BAM_CHARD_CLIP: - schar = 'H'; - break; - case BAM_CSOFT_CLIP: // skip - schar = 'S'; - break; - case BAM_CMATCH: - schar = 'M'; - break; - case BAM_CEQUAL: - schar = '='; - break; - case BAM_CDIFF: - schar = '#'; - break; - case BAM_CINS: - schar = 'I'; - break; - case BAM_CDEL: - schar = 'I'; - break; - default: - schar = '?'; + char* qry_rev = qry_mem (realigner, qrybases->l+1); + memcpy (qry_rev, qry, qrybases->l); + qry_rev [qrybases->l] = 0; + tmap_reverse_compliment (qry_rev, (int32_t) qrybases->l); + qry = qry_rev; } - tmap_log ("%c%d", schar, count); + // compute alignment and subject lengths + unsigned q_len_cigar, r_len_cigar; + seq_lens_from_bin_cigar (orig_cigar, orig_cigar_sz, &q_len_cigar, &r_len_cigar); + uint8_t* ref = (uint8_t*) ref_mem (realigner, r_len_cigar); + int32_t converted_cnt; + // extract reference. This returns reference sequence in UNPACKED but BINARY CONVERTED form - values of 0-4, one byte per base! + tmap_refseq_subseq2 (refseq, ref_id+1, ref_off+1, ref_off + r_len_cigar, ref, 0, &converted_cnt); + // convert reference to ascii format + { + int ii; + for (ii = 0; ii < r_len_cigar; ++ii) + { + if (ref [ii] >= sizeof (tmap_iupac_int_to_char)) + tmap_bug (); + ref [ii] = tmap_iupac_int_to_char [ref [ii]]; + } + ref [r_len_cigar] = 0; + } + // get name for reporting + const char* ref_name = refseq->annos [ref_id].name->s; + // prepare variables to hold results + uint32_t* cigar_dest; + unsigned cigar_dest_sz; + unsigned new_ref_offset, new_qry_offset, new_ref_len, new_qry_len; + ++(stat->num_realign_invocations); + uint64_t apo = stat->num_realign_already_perfect; + uint64_t nco = stat->num_realign_not_clipped; + uint64_t swfo = stat->num_realign_sw_failures; + uint64_t ucfo = stat->num_realign_unclip_failures; + const char* al_proc_class = "UNKNOWN"; + // compute the alignment + uint8_t al_mod = 0; + if (realigner_compute_alignment (realigner, + qry, + qrybases->l, + (const char*) ref, + r_len_cigar, + ref_off, + forward, + orig_cigar, + orig_cigar_sz, + &cigar_dest, + &cigar_dest_sz, + &new_ref_offset, + &new_qry_offset, + &new_ref_len, + &new_qry_len, + &(stat->num_realign_already_perfect), + &(stat->num_realign_not_clipped), + &(stat->num_realign_sw_failures), + &(stat->num_realign_unclip_failures))) + { + // fix alignment end softclip - the realignment may skip some of it + int32_t sc_adj = qrybases->l - new_qry_offset - new_qry_len; + if (sc_adj) do + { + if ((cigar_dest_sz != 0) && (TMAP_SW_CIGAR_OP (cigar_dest [cigar_dest_sz - 1]) == BAM_CSOFT_CLIP)) + { + sc_adj += TMAP_SW_CIGAR_LENGTH (cigar_dest [cigar_dest_sz - 1]); + TMAP_SW_CIGAR_STORE (cigar_dest [cigar_dest_sz - 1], BAM_CSOFT_CLIP, sc_adj); + break; + } + ++cigar_dest_sz; + cigar_dest = tmap_realloc (cigar_dest, cigar_dest_sz * sizeof (*(match->cigar)), "cigar_dest"); + TMAP_SW_CIGAR_STORE (cigar_dest [cigar_dest_sz - 1], BAM_CSOFT_CLIP, sc_adj); + } + while (0); + // check if changes were introduced + if (new_ref_offset != ref_off || orig_cigar_sz != cigar_dest_sz || memcmp (orig_cigar, cigar_dest, orig_cigar_sz * sizeof (*orig_cigar))) + { + ++(stat->num_realign_changed); + if (new_ref_offset != ref_off) + { + // log shifted alignment + ++(stat->num_realign_shifted); + if (nco == stat->num_realign_not_clipped) + al_proc_class = "MOD-SHIFT"; + else + al_proc_class = "MOD-SHIFT NOCLIP"; + } + else + { + // log changed alignment + if (nco == stat->num_realign_not_clipped) + al_proc_class = "MOD"; + else + al_proc_class = "MOD NOCLIP"; + } + al_mod = 1; + // pack the alignment back into the originating structure (unallocate memory taken by the old one) + // TODO implement more intelligent alignment memory management, avoid heap operations when possible + free (match->cigar); + match->cigar = cigar_dest; + match->n_cigar = cigar_dest_sz; + match->pos = new_ref_offset; + // we need to update match->result since edge indel salvage relies on it + match->result.query_start = new_qry_offset; + match->result.query_end = new_qry_offset + new_qry_len - 1; + match->result.target_start = 0; // adjusted!new_ref_offset - ref_off; + match->result.target_end = new_ref_len - 1; + match->target_len = new_ref_len; + } + else + { + // log unchanged alignment + free (cigar_dest); + if (nco == stat->num_realign_not_clipped) + al_proc_class = "UNMOD"; + else + al_proc_class = "UNMOD NOCLIP"; + } + } + else + { + // log reason why alignment was not processed + if (swfo != stat->num_realign_sw_failures) + al_proc_class = "SWERR"; + else if (ucfo != stat->num_realign_unclip_failures) + al_proc_class = "UNCLIPERR"; + else if (apo != stat->num_realign_already_perfect) + al_proc_class = "PERFECT"; + } + // log + tmap_log_record_begin (); + tmap_log ("REALIGN: %s(%s) vs %s:%d %s. ", qryname, (forward ? "FWD":"REV"), ref_name, ref_off, al_proc_class); + if (al_mod) + { + tmap_log ("orig (%d op) at %d: ", orig_cigar_sz, ref_off); + cigar_log (orig_cigar, orig_cigar_sz); + tmap_log ("; new (%d op) at %d: ", cigar_dest_sz, new_ref_offset); + cigar_log (cigar_dest, cigar_dest_sz); + + if (log_text_als) + { + tmap_map_log_text_align (" Before realignment:\n", orig_cigar, orig_cigar_sz, qry, qrybases->l, forward, (const char*) ref, ref_off); + tmap_map_log_text_align (" After realignment:\n", match->cigar, match->n_cigar, qry, qrybases->l, forward, (const char*) (ref + (new_ref_offset - ref_off)), new_ref_offset); + } + } + tmap_log ("\n"); + tmap_log_record_end (); + free (orig_cigar); } } -typedef struct -{ - unsigned xpos; - unsigned ypos; - unsigned len; -} AlBatch; - -static uint32_t cigar_to_batches (const uint32_t* cigar, uint32_t cigar_sz, uint32_t* x_clip, AlBatch* batches, uint32_t max_batches) +void context_align_read (tmap_seq_t* qryseq, tmap_refseq_t* refseq, tmap_map_sams_t* sams, struct RealignProxy* context, tmap_map_stats_t *stat, int32_t log_text_als) { - uint32_t xpos = 0, ypos = 0; // x is query, y is reference - AlBatch* curb = batches; - const uint32_t* sent = cigar + cigar_sz; - for (; cigar != sent && curb - batches != max_batches; ++cigar) + // context_align_read (qryseq, refseq, sams) + // extract query + const char* qryname = tmap_seq_get_name (qryseq)->s; + + tmap_string_t* qrybases = tmap_seq_get_bases (qryseq); + const char* qry = qrybases->s; + uint32_t qry_len = qrybases->l; + unsigned matchidx; + for (matchidx = 0; matchidx < sams->n; ++matchidx) { - uint32_t curop = bam_cigar_op (*cigar); - uint32_t count = bam_cigar_oplen (*cigar); - switch (curop) + // extract packed cigar and it's length + tmap_map_sam_t* match = sams->sams + matchidx; + uint32_t* orig_cigar = match->cigar; + unsigned orig_cigar_sz = match->n_cigar; + // extract seqid + unsigned ref_id = match->seqid; + // extract offset + unsigned ref_off = match->pos; + // extract read direction in alignment + uint8_t forward = (match->strand == 0) ? 1 : 0; + // make inverse/complement copy of the query if direction is reverse + if (!forward) { - case BAM_CHARD_CLIP: // skip - case BAM_CSOFT_CLIP: // skip - if (x_clip) *x_clip += count; - break; - case BAM_CMATCH: - case BAM_CEQUAL: - case BAM_CDIFF: - curb->xpos = xpos; - curb->ypos = ypos; - curb->len = count; - xpos += count; - ypos += count; - ++curb; - x_clip = NULL; - break; - case BAM_CINS: - xpos += count; - x_clip = NULL; - break; - case BAM_CDEL: - ypos += count; - x_clip = NULL; - break; - default: - ; + char* qry_rev = qry_mem (context, qrybases->l+1); + memcpy (qry_rev, qry, qrybases->l); + qry_rev [qrybases->l] = 0; + tmap_reverse_compliment (qry_rev, (int32_t) qrybases->l); + qry = qry_rev; } - } - return curb - batches; -} - -#define MAXSTRLEN 256 -#define NUMSTRLEN 11 + // compute alignment and subject lengths + unsigned q_len_cigar, r_len_cigar; + seq_lens_from_bin_cigar (orig_cigar, orig_cigar_sz, &q_len_cigar, &r_len_cigar); + uint8_t* ref = (uint8_t*) ref_mem (context, r_len_cigar+1); + int32_t converted_cnt; + // extract reference. This returns reference sequence in UNPACKED but BINARY CONVERTED form - values of 0-4, one byte per base! + if (ref != tmap_refseq_subseq2 (refseq, ref_id+1, ref_off+1, ref_off + r_len_cigar, ref, 0, &converted_cnt)) + tmap_bug (); + // convert reference to ascii format + { + int ii; + for(ii = 0; ii < r_len_cigar; ++ii) + { + if (ref [ii] >= sizeof (tmap_iupac_int_to_char)) + tmap_bug (); + ref [ii] = tmap_iupac_int_to_char [ref [ii]]; + } + ref [r_len_cigar] = 0; + } + const char* ref_name = refseq->annos [ref_id].name->s; + // prepare variables to hold results + uint32_t* cigar_dest; + unsigned cigar_dest_sz; + unsigned new_ref_offset, new_qry_offset, new_ref_len, new_qry_len; + ++(stat->num_hpcost_invocations); + // compute the alignment + + if (realigner_compute_alignment (context, + qry, + qry_len, + (const char*) ref, + r_len_cigar, + ref_off, + forward, + orig_cigar, + orig_cigar_sz, + &cigar_dest, + &cigar_dest_sz, + &new_ref_offset, + &new_qry_offset, + &new_ref_len, + &new_qry_len, + NULL, + NULL, + NULL, + NULL)) + { + // check if changes were introduced + if (new_ref_offset != ref_off || orig_cigar_sz != cigar_dest_sz || memcmp (orig_cigar, cigar_dest, orig_cigar_sz*sizeof (*orig_cigar))) + { + if (tmap_log_enabled ()) + { + tmap_log_record_begin (); + tmap_log ("CONTEXT-GAP: %s(%s) vs %s:%d MODIFIED: ", qryname, (forward ? "FWD":"REV"), ref_name, ref_off); + tmap_log ("orig (%d op) at %d: ", orig_cigar_sz, ref_off); + cigar_log (orig_cigar, orig_cigar_sz); + tmap_log ("; new (%d op) at %d: ", cigar_dest_sz, new_ref_offset); + cigar_log (cigar_dest, cigar_dest_sz); + tmap_log ("\n"); + + if (log_text_als) + { + tmap_map_log_text_align (" Before context realignment:\n", orig_cigar, orig_cigar_sz, qry, qrybases->l, forward, (const char*) ref, ref_off); + tmap_map_log_text_align (" After context realignment:\n", cigar_dest, cigar_dest_sz, qry, qrybases->l, forward, (const char*) (ref + (new_ref_offset - ref_off)), new_ref_offset); + } + tmap_log_record_end (); + } -void log_batches (const char* xseq, unsigned xlen, uint8_t xrev, const char* yseq, unsigned ylen, uint8_t yrev, const AlBatch *b_ptr, int b_cnt, unsigned xoff, unsigned yoff) -{ - const unsigned margin = 0, width = 120, zero_based = 1; + ++(stat->num_hpcost_modified); + if (new_ref_offset != ref_off) + // log shifted alignment + ++(stat->num_hpcost_shifted); + // pack the alignment back into the originating structure (unallocate memory taken by the old one) + if (orig_cigar_sz < cigar_dest_sz) + { + free (match->cigar); + match->cigar = (uint32_t*) tmap_malloc (sizeof (uint32_t) * cigar_dest_sz, "cigar_dest"); + } + match->n_cigar = cigar_dest_sz; + memcpy (match->cigar, cigar_dest, sizeof (uint32_t) * cigar_dest_sz); + sams->sams [matchidx].pos = new_ref_offset; + // we need to update match->result since edge indel salvage relies on it + match->result.query_start = new_qry_offset; + match->result.query_end = new_qry_offset + new_qry_len - 1; + match->result.target_start = 0; // adjusted!new_ref_offset - ref_off; + match->result.target_end = new_ref_len - 1; + match->target_len = new_ref_len; - unsigned slen = 0; - unsigned blen = 0; - unsigned x = b_ptr->xpos; - unsigned y = b_ptr->ypos; - unsigned xstart = x; - unsigned ystart = y; - unsigned char xc, yc; - char s[3][MAXSTRLEN]; + } + else + tmap_log_s ("CONTEXT-GAP: %s(%s) vs %s:%d UNCHANGED\n", qryname, (forward ? "FWD":"REV"), ref_name, ref_off); + } + else + { + ++(stat->num_hpcost_skipped); + tmap_log_s ("CONTEXT-GAP: %s(%s) vs %s:%d SKIPPED\n", qryname, (forward ? "FWD":"REV"), ref_name, ref_off); + } + } - assert (width < MAXSTRLEN); +} +const unsigned MAX_PERIOD = 10; - while (b_cnt > 0) +void tail_repeats_clip_read (tmap_seq_t* qryseq, tmap_refseq_t* refseq, tmap_map_sams_t* sams, struct RealignProxy* realigner, tmap_map_stats_t *stat, int32_t repclip_continuation) +{ + // extract query + const char* qryname = tmap_seq_get_name (qryseq)->s; + tmap_string_t* qrybases = tmap_seq_get_bases (qryseq); + const char* qry = qrybases->s; + unsigned filtered = 0; // some of the alignments could be completely skipped + + unsigned matchidx; + for (matchidx = 0; matchidx < sams->n; ++matchidx) { - xc = xseq [x]; - yc = yseq [y]; - - // special handling for (x < b_ptr->xpos && y < b_ptr->ypos) - // treating as batch with special match symbols - - if (x < b_ptr->xpos && y < b_ptr->ypos) + // if necessary, prepare slot for filling in the sam + if (filtered != matchidx) + { + // if we had rejected some of matches, the tail should shrink. (avoid reallocation, just swap all internal pointers. Otherwise it'll be too heavy, and it'll be better to skip them during BAM writing, which is logically more cumbersome) + tmap_map_sam_t tmp = sams->sams [filtered]; + sams->sams [filtered] = sams->sams [matchidx]; + sams->sams [matchidx] = tmp; + } + // extract packed cigar and it's length + uint32_t* orig_cigar = sams->sams [filtered].cigar; + unsigned orig_cigar_sz = sams->sams [filtered].n_cigar; + // extract seqid + unsigned ref_id = sams->sams [filtered].seqid; + // extract offset + unsigned ref_off = sams->sams [filtered].pos; + // extract read direction in alignment + uint8_t forward = (sams->sams [filtered].strand == 0) ? 1 : 0; + // compute alignment and subject lengths + unsigned q_al_beg, q_al_end, r_al_beg, r_al_end; + alignment_bounds_from_bin_cigar (orig_cigar, orig_cigar_sz, forward, qrybases->l, &q_al_beg, &q_al_end, &r_al_beg, &r_al_end); + // check if this read had hit the adapter (even one-base hit makes the repeat clipping invalid) + if (tmap_sam_get_zb (qryseq->data.sam) > 0 && tmap_sam_get_za (qryseq->data.sam) <= q_al_end) { - s[0][slen] = xc; - s[2][slen] = yc; - s[1][slen] = '#'; - x++, y++, slen++; + filtered ++; + continue; } - // X insert - else if (x < b_ptr->xpos) + const char* ref_name = refseq->annos [ref_id].name->s; + unsigned qry_rep_off; + if (repclip_continuation) { - s[0][slen] = xc; - s[1][slen] = ' '; - s[2][slen] = '-'; - x++, slen++; + // extract reference 'tail' (continuation). This returns reference sequence in UNPACKED but BINARY CONVERTED form - values of 0-4, one byte per base! + int32_t ref_tail_beg = forward ? (ref_off + r_al_end) : ((MAX_PERIOD < ref_off + r_al_beg) ? (ref_off + r_al_beg - MAX_PERIOD) : 0); + int32_t ref_tail_end = forward ? mymin (refseq->annos [ref_id].len, ref_tail_beg + MAX_PERIOD) : (ref_off + r_al_beg); //inclusive 1-based - same as exclusive 0-based + int32_t ref_tail_len = ref_tail_end - ref_tail_beg; + if (!ref_tail_len) + { + filtered ++; + continue; + } + uint8_t* ref = (uint8_t*) ref_mem (realigner, ref_tail_len); + int32_t converted_cnt; + tmap_refseq_subseq2 (refseq, ref_id+1, ref_tail_beg+1, ref_tail_end, ref, 0, &converted_cnt); + // convert reference to ascii format + { + int ii; + for(ii = 0; ii < ref_tail_len; ++ii) + { + if (ref [ii] >= sizeof (tmap_iupac_int_to_char)) + { + fprintf (stderr, "Invalid base: %d at position %d in reference %s:%d-%d\n", ref [ii], ii, ref_name, ref_tail_beg+1, ref_tail_end); + tmap_bug (); + } + ref [ii] = tmap_iupac_int_to_char [ref [ii]]; + } + ref [ref_tail_len] = 0; + } + // inverse/complement for reverse match + if (!forward) + tmap_reverse_compliment ((char*) ref, ref_tail_len); + // check if there is a tail periodicity extending by at least one period into reference overhang; + // tailrep returns 0 if entire (unclipped) alignment gets trimmed + qry_rep_off = tailrep_cont (ref_tail_len, (char*) ref, q_al_end, qry); } - // Y insert - else if (y < b_ptr->ypos) + else { - s[0][slen] = '-'; - s[1][slen] = ' '; - s[2][slen] = yc; - y++, slen++; + qry_rep_off = tailrep (q_al_end, qry, MAX_PERIOD); } - // emit text batch - else if (blen < b_ptr->len) + // qry_rep_off is where ON THE READ (in read's forward direction) the clip should start. + ++ stat->num_seen_tailclipped; + stat->bases_seen_tailclipped += q_al_end; + // soft-clip longest one, if any + if (qry_rep_off != q_al_end) { - s[0][slen] = xc; - s[2][slen] = yc; - s[1][slen] = (toupper (xc) == toupper (yc) || toupper (xc) == 'N' || toupper (yc) == 'N') ? '*' : ' '; - x++, y++, slen++, blen++; + // save original cigar for reporting + uint32_t* orig_cigar = alloca (sizeof (uint32_t) * orig_cigar_sz); + memcpy (orig_cigar, sams->sams [filtered].cigar, sizeof (uint32_t) * orig_cigar_sz); + unsigned ref_rep_off = 0; + unsigned trimmed_cigar_sz = trim_cigar (&(sams->sams [filtered].cigar), orig_cigar_sz, qrybases->l, qry_rep_off, forward, &ref_rep_off); + // if (trimmed_cigar_sz) + if (trimmed_cigar_sz && !fully_clipped (sams->sams [filtered].cigar, trimmed_cigar_sz)) + { + sams->sams [filtered].n_cigar = trimmed_cigar_sz; + if (!forward) + sams->sams [filtered].pos += ref_rep_off; + + tmap_log_record_begin (); + tmap_log ("TAIL_REP_TRIM: %s(%s) vs %s:%d TRIMMED %d bases. ", qryname, (forward ? "FWD":"REV"), ref_name, ref_off, q_al_end - qry_rep_off); + tmap_log ("orig (%d op) at %d: ", orig_cigar_sz, ref_off); + cigar_log (orig_cigar, orig_cigar_sz); + tmap_log ("; new (%d op) at %d: ", trimmed_cigar_sz, sams->sams [filtered].pos); + cigar_log (sams->sams [filtered].cigar, trimmed_cigar_sz); + tmap_log ("\n"); + tmap_log_record_end (); + + stat->bases_tailclipped += q_al_end - qry_rep_off; // with respect to the read + filtered ++; + } + else + { + tmap_log_s ("TAIL_REP_TRIM: %s(%s) vs %s:%d REMOVED (FULLY TRIMMED)\n", qryname, (forward ? "FWD":"REV"), ref_name, ref_off); + stat->bases_tailclipped += q_al_end; + stat->bases_fully_tailclipped += q_al_end; + ++ stat->num_fully_tailclipped; + // do not increment filtered; skip this + } + ++ stat->num_tailclipped; } else - blen = 0, b_cnt--, b_ptr++; - - //print accumulated lines - if ((slen + NUMSTRLEN > width) || b_cnt <= 0) { - //null terminate all strings - for (int i = 0; i < 3; i++) - s[i][slen] = 0; + tmap_log_s ("TAIL_REP_TRIM: %s(%s) vs %s:%d UNCHANGED\n", qryname, (forward ? "FWD":"REV"), ref_name, ref_off); + ++ filtered; + } + } // end alignment processing + sams->n = filtered; +} - unsigned xdisp = (xrev ? xlen - xstart - 1 : xstart) + xoff + (zero_based ? 0 : 1); - unsigned ydisp = (yrev ? ylen - ystart - 1 : ystart) + yoff + (zero_based ? 0 : 1); +void realign_reads (tmap_seqs_t *seqs_buffer, tmap_refseq_t* refseq, tmap_map_record_t* record, struct RealignProxy* realigner, tmap_map_stats_t *stat, int32_t log_text_als) +{ + unsigned seqidx; + for (seqidx = 0; seqidx < seqs_buffer->n; ++seqidx) + { + tmap_map_sams_t* sams = record->sams [seqidx]; + tmap_seq_t* qryseq = seqs_buffer->seqs [seqidx]; + realign_read (qryseq, refseq, sams, realigner, stat, log_text_als); + } +} - tmap_log ("\n%*s%*d %s", margin, "", NUMSTRLEN, xdisp, s[0]); - tmap_log ("\n%*s%*s %s", margin, "", NUMSTRLEN, "", s[1]); - tmap_log ("\n%*s%*d %s\n", margin, "", NUMSTRLEN, ydisp, s[2]); +void context_align_reads (tmap_seqs_t *seqs_buffer, tmap_refseq_t* refseq, tmap_map_record_t* record, struct RealignProxy* realigner, tmap_map_stats_t *stat, int32_t log_text_als) +{ + unsigned seqidx; + for (seqidx = 0; seqidx < seqs_buffer->n; ++seqidx) + { + tmap_map_sams_t* sams = record->sams [seqidx]; + tmap_seq_t* qryseq = seqs_buffer->seqs [seqidx]; + context_align_read (qryseq, refseq, sams, realigner, stat, log_text_als); + } +} - xstart = x, ystart = y, slen = 0; - } +void tail_repeat_clip_reads (tmap_seqs_t *seqs_buffer, tmap_refseq_t* refseq, tmap_map_record_t* record, struct RealignProxy* realigner, tmap_map_stats_t *stat, int32_t repclip_continuation) +{ + unsigned seqidx; + for (seqidx = 0; seqidx < seqs_buffer->n; ++seqidx) + { + tmap_map_sams_t* sams = record->sams [seqidx]; + tmap_seq_t* qryseq = seqs_buffer->seqs [seqidx]; + tail_repeats_clip_read (qryseq, refseq, sams, realigner, stat, repclip_continuation); } } @@ -674,755 +986,451 @@ tmap_map_driver_core_worker(sam_header_t *sam_header, int32_t do_pairing, int32_t tid) { - int32_t i, j, k, low = 0; - int32_t found; - tmap_seq_t ***seqs = NULL; - tmap_bwt_match_hash_t *hash=NULL; - int32_t max_num_ends = 0; - -#ifdef TMAP_DRIVER_USE_HASH - // init the occurence hash - hash = tmap_bwt_match_hash_init(); -#endif - - // init memory - max_num_ends = 2; - seqs = tmap_malloc(sizeof(tmap_seq_t**)*max_num_ends, "seqs"); - for(i=0;iopt->num_threads)) { - tmap_map_stats_t *stage_stat = NULL; - tmap_map_record_t *record_prev = NULL; - int32_t num_ends; - -#ifdef TMAP_DRIVER_USE_HASH -#ifdef TMAP_DRIVER_CLEAR_HASH_PER_READ - // TODO: should we hash each read, or across the thread? - tmap_bwt_match_hash_clear(hash); -#endif -#endif + int32_t i, j, k, low = 0; + int32_t found; + tmap_seq_t*** seqs = NULL; + tmap_bwt_match_hash_t* hash=NULL; + int32_t max_num_ends = 0; + + // common memory resource for all target fragments + // common memory resource for WS traceback paths + tmap_sw_path_t *path_buf = NULL; // buffer for traceback path + int32_t path_buf_sz = 0; // used portion and allocated size of traceback path. + + #ifdef TMAP_DRIVER_USE_HASH + // init the occurence hash + hash = tmap_bwt_match_hash_init (); + #endif + + // init memory + max_num_ends = 2; + seqs = tmap_malloc (sizeof (tmap_seq_t**) * max_num_ends, "seqs"); + for (i = 0; i < max_num_ends; i++) + seqs [i] = tmap_calloc (4, sizeof (tmap_seq_t*), "seqs[i]"); + + // init target sequence cache + ref_buf_t target; + target_cache_init (&target); + + // initialize thread data + tmap_map_driver_do_threads_init (driver, tid); + + // Go through the buffer + while (low < seqs_buffer_length) + { + if (tid == (low % driver->opt->num_threads)) + { + tmap_map_stats_t *stage_stat = NULL; + tmap_map_record_t *record_prev = NULL; + int32_t num_ends; + + #ifdef TMAP_DRIVER_USE_HASH + #ifdef TMAP_DRIVER_CLEAR_HASH_PER_READ + // TODO: should we hash each read, or across the thread? + tmap_bwt_match_hash_clear (hash); + #endif + #endif + + num_ends = seqs_buffer [low]->n; + if(max_num_ends < num_ends) + { + seqs = tmap_realloc (seqs, sizeof (tmap_seq_t**) * num_ends, "seqs"); + while(max_num_ends < num_ends) + { + seqs [max_num_ends] = tmap_calloc(4, sizeof(tmap_seq_t*), "seqs[max_num_ends]"); + max_num_ends++; + } + max_num_ends = num_ends; + } + // re-initialize the random seed + if(driver->opt->rand_read_name) + tmap_rand_reinit(rand, tmap_hash_str_hash_func_exc(tmap_seq_get_name(seqs_buffer[low]->seqs[0])->s, driver->opt->prefix_exclude, driver->opt->suffix_exclude)); - num_ends = seqs_buffer[low]->n; - if(max_num_ends < num_ends) { - seqs = tmap_realloc(seqs, sizeof(tmap_seq_t**)*num_ends, "seqs"); - while(max_num_ends < num_ends) { - seqs[max_num_ends] = tmap_calloc(4, sizeof(tmap_seq_t*), "seqs[max_num_ends]"); - max_num_ends++; - } - max_num_ends = num_ends; - } - // re-initialize the random seed - if(driver->opt->rand_read_name) { - tmap_rand_reinit(rand, tmap_hash_str_hash_func_exc(tmap_seq_get_name(seqs_buffer[low]->seqs[0])->s, driver->opt->prefix_exclude, driver->opt->suffix_exclude)); - } + // init + for(i = 0; i < num_ends; i++) + { + tmap_map_driver_init_seqs (seqs [i], seqs_buffer [low]->seqs [i], -1); + if (NULL != stat) stat->num_reads++; + } - // init - for(i=0;iseqs[i], -1); - if(NULL != stat) stat->num_reads++; - } + // init records + records [low] = tmap_map_record_init (num_ends); - // init records - records[low] = tmap_map_record_init(num_ends); + // go through each stage + for (i = 0; i < driver->num_stages; i++) + { // for each stage - // go through each stage - for(i=0;inum_stages;i++) { // for each stage - tmap_map_driver_stage_t *stage = driver->stages[i]; + tmap_map_driver_stage_t *stage = driver->stages [i]; + tmap_sw_param_t sw_par; + // stage may have special sw parameters + tmap_map_util_populate_sw_par (&sw_par, stage->opt); - // stage stats - stage_stat = tmap_map_stats_init(); + // stage stats + stage_stat = tmap_map_stats_init (); - // seed - for(j=0;jopt->stage_seed_max_length && stage->opt->stage_seed_max_length < tmap_seq_get_bases_length(seqs[j][0])) { - stage_seqs = tmap_calloc(4, sizeof(tmap_seq_t*), "seqs[i]"); - tmap_map_driver_init_seqs(stage_seqs, seqs_buffer[low]->seqs[i], stage->opt->stage_seed_max_length); - } - else { - stage_seqs = seqs[j]; - } - for(k=0;knum_algorithms;k++) { // for each algorithm - tmap_map_driver_algorithm_t *algorithm = stage->algorithms[k]; - tmap_map_sams_t *sams = NULL; - if(i+1 != algorithm->opt->algo_stage) { - tmap_bug(); - } - // map - sams = algorithm->func_thread_map(&algorithm->thread_data[tid], stage_seqs, index, hash, rand, algorithm->opt); - if(NULL == sams) { - tmap_error("the thread function did not return a mapping", Exit, OutOfRange); - } - // append - tmap_map_sams_merge(records[low]->sams[j], sams); - // destroy - tmap_map_sams_destroy(sams); - } - stage_stat->num_after_seeding += records[low]->sams[j]->n; - if(0 < stage->opt->stage_seed_max_length && stage->opt->stage_seed_max_length < tmap_seq_get_bases_length(seqs[j][0])) { - // free - for(j=0;j<4;j++) { - tmap_seq_destroy(stage_seqs[j]); - stage_seqs[j] = NULL; - } - } - stage_seqs = NULL; // do not use - } + // seed + for (j = 0; j < num_ends; j++) + { // for each end + tmap_seq_t** stage_seqs = NULL; + // should we seed using the whole read? + if(0 < stage->opt->stage_seed_max_length && stage->opt->stage_seed_max_length < tmap_seq_get_bases_length (seqs [j][0])) + { + stage_seqs = tmap_calloc (4, sizeof (tmap_seq_t*), "seqs[i]"); + tmap_map_driver_init_seqs (stage_seqs, seqs_buffer [low]->seqs [i], stage->opt->stage_seed_max_length); + } + else + stage_seqs = seqs [j]; + for (k = 0; k < stage->num_algorithms; k++) + { // for each algorithm + tmap_map_driver_algorithm_t *algorithm = stage->algorithms [k]; + tmap_map_sams_t *sams = NULL; + if (i + 1 != algorithm->opt->algo_stage) + tmap_bug(); + // map + sams = algorithm->func_thread_map (&algorithm->thread_data [tid], stage_seqs, index, hash, rand, algorithm->opt); + if (NULL == sams) + tmap_error ("the thread function did not return a mapping", Exit, OutOfRange); + // append + tmap_map_sams_merge (records [low]->sams [j], sams); + // destroy + tmap_map_sams_destroy (sams); + } + stage_stat->num_after_seeding += records [low]->sams [j]->n; + if (0 < stage->opt->stage_seed_max_length && stage->opt->stage_seed_max_length < tmap_seq_get_bases_length (seqs [j][0])) + { + // free + for (j = 0; j < 4; j++) + { + tmap_seq_destroy (stage_seqs [j]); + stage_seqs [j] = NULL; + } + } + stage_seqs = NULL; // do not use + } - // keep mappings for subsequent stages or restore mappings from - // previous stages - if(1 == stage->opt->stage_keep_all) { - // merge from the previous stage - if(0 < i) { - tmap_map_record_merge(records[low], record_prev); - // destroy the record - tmap_map_record_destroy(record_prev); - record_prev = NULL; - } + // keep mappings for subsequent stages or restore mappings from + // previous stages + if (1 == stage->opt->stage_keep_all) + { + // merge from the previous stage + if (0 < i) + { + tmap_map_record_merge (records [low], record_prev); + // destroy the record + tmap_map_record_destroy (record_prev); + record_prev = NULL; + } - // keep for the next stage - if(i < driver->num_stages-1) { // more stages left - record_prev = tmap_map_record_clone(records[low]); - } - } + // keep for the next stage + if (i < driver->num_stages - 1) // more stages left + record_prev = tmap_map_record_clone (records [low]); + } - // generate scores with smith waterman - for(j=0;jsams[j] = tmap_map_util_sw_gen_score(index->refseq, seqs_buffer[low]->seqs[j], records[low]->sams[j], seqs[j], rand, stage->opt, &k); - stage_stat->num_after_scoring += records[low]->sams[j]->n; - stage_stat->num_after_grouping += k; - } + // generate scores with smith waterman + for (j = 0; j < num_ends; j++) + { // for each end + records [low]->sams [j] = tmap_map_util_sw_gen_score (index->refseq, seqs_buffer [low]->seqs [j], records [low]->sams [j], seqs [j], rand, stage->opt, &k); + stage_stat->num_after_scoring += records [low]->sams [j]->n; + stage_stat->num_after_grouping += k; + } - // remove duplicates - for(j=0;jsams[j], stage->opt->dup_window, rand); - stage_stat->num_after_rmdup += records[low]->sams[j]->n; - } + // remove duplicates + for (j = 0; j < num_ends; j++) + { // for each end + tmap_map_util_remove_duplicates (records [low]->sams [j], stage->opt->dup_window, rand); + stage_stat->num_after_rmdup += records [low]->sams [j]->n; + } - // (single-end) mapping quality - for(j=0;jfunc_mapq(records[low]->sams[j], tmap_seq_get_bases_length(seqs[j][0]), stage->opt, index->refseq); - } + // (single-end) mapping quality + for (j = 0;j < num_ends; j++) // for each end + driver->func_mapq (records [low]->sams [j], tmap_seq_get_bases_length (seqs [j][0]), stage->opt, index->refseq); - // filter if we have more stages - if(i < driver->num_stages-1) { - for(j=0;jsams[j], stage->opt->stage_score_thr, stage->opt->stage_mapq_thr); - } - } - if(0 == do_pairing && 0 <= driver->opt->strandedness && 0 <= driver->opt->positioning - && 2 == num_ends && 0 < records[low]->sams[0]->n && 0 < records[low]->sams[1]->n) { // pairs of reads! + // filter if we have more stages + if (i < driver->num_stages-1) + { + for (j = 0; j < num_ends; j++) // for each end + tmap_map_sams_filter2 (records [low]->sams [j], stage->opt->stage_score_thr, stage->opt->stage_mapq_thr); + } - // read rescue - if(1 == stage->opt->read_rescue) { - int32_t flag = tmap_map_pairing_read_rescue(index->refseq, - seqs_buffer[low]->seqs[0], seqs_buffer[low]->seqs[1], - records[low]->sams[0], records[low]->sams[1], - seqs[0], seqs[1], + if (0 == do_pairing && 0 <= driver->opt->strandedness && 0 <= driver->opt->positioning + && 2 == num_ends && 0 < records [low]->sams [0]->n && 0 < records [low]->sams [1]->n) + { // pairs of reads! + // read rescue + if (1 == stage->opt->read_rescue) + { + int32_t flag = tmap_map_pairing_read_rescue (index->refseq, + seqs_buffer [low]->seqs[0], seqs_buffer [low]->seqs [1], + records [low]->sams [0], records [low]->sams [1], + seqs [0], seqs [1], rand, stage->opt); - // recalculate mapping qualities if necessary - if(0 < (flag & 0x1)) { // first end was rescued - //fprintf(stderr, "re-doing mapq for end #1\n"); - driver->func_mapq(records[low]->sams[0], tmap_seq_get_bases_length(seqs[0][0]), stage->opt, index->refseq); - } - if(0 < (flag & 0x2)) { // second end was rescued - //fprintf(stderr, "re-doing mapq for end #2\n"); - driver->func_mapq(records[low]->sams[1], tmap_seq_get_bases_length(seqs[1][0]), stage->opt, index->refseq); - } - } - // pick pairs - tmap_map_pairing_pick_pairs(records[low]->sams[0], records[low]->sams[1], - seqs[0][0], seqs[1][0], + // recalculate mapping qualities if necessary + if (0 < (flag & 0x1)) // first end was rescued + driver->func_mapq (records [low]->sams [0], tmap_seq_get_bases_length (seqs [0][0]), stage->opt, index->refseq); + if(0 < (flag & 0x2)) // second end was rescued + driver->func_mapq (records [low]->sams [1], tmap_seq_get_bases_length (seqs [1][0]), stage->opt, index->refseq); + } + // pick pairs + tmap_map_pairing_pick_pairs (records [low]->sams [0], records [low]->sams [1], + seqs [0][0], seqs [1][0], rand, stage->opt); - // TODO: if we have one end for a pair, do we go onto the second - // stage? - } - else { - // choose alignments - for(j=0;jsams[j], stage->opt->aln_output_mode, TMAP_MAP_ALGO_NONE, rand); - stage_stat->num_after_filter += records[low]->sams[j]->n; - } - } - - // generate the cigars - found = 0; - for(j=0;jsams[j] = tmap_map_util_sw_gen_cigar(index->refseq, records[low]->sams[j], seqs_buffer[low]->seqs[j], seqs[j], stage->opt); - if(0 < records[low]->sams[j]->n) { - stage_stat->num_with_mapping++; - found = 1; - } - } - - // TODO - // if paired, update pairing score based on target start? - - // did we find any mappings? - if(1 == found) { // yes - if(NULL != stat) { - tmap_map_stats_add(stat, stage_stat); - } - tmap_map_stats_destroy(stage_stat); - break; - } - else { // no - tmap_map_record_destroy(records[low]); - // re-init - records[low] = tmap_map_record_init(num_ends); - } - tmap_map_stats_destroy(stage_stat); - } - - // flowspace re-align and sorting - if(1 == driver->opt->aln_flowspace) { - for(i=0;isams[i]->n) { - // re-align the alignments in flow-space - tmap_seq_t *seq = seqs_buffer[low]->seqs[i]; - // TODO: if this is run, we do not need to run tmap_sw_global_banded_core... - // NB: seqs_buffer should have its key sequence if 0 < key_seq_len - if(1 == tmap_map_util_fsw(seq, records[low]->sams[i], index->refseq, - driver->opt->bw, driver->opt->softclip_type, driver->opt->score_thr, - driver->opt->score_match, driver->opt->pen_mm, driver->opt->pen_gapo, - driver->opt->pen_gape, driver->opt->fscore, 1-driver->opt->ignore_flowgram)) { - // sort by alignment score - if(1 < records[low]->sams[i]->n) { - tmap_sort_introsort(tmap_map_sam_sort_score, - records[low]->sams[i]->n, - records[low]->sams[i]->sams); - } - } - } - if(NULL == seqs_buffer[low]->seqs[i]) { - tmap_error("bug encoutereed", Exit, OutOfRange); - } - } - } - - // only convert to BAM and destroy the records if we are not trying to - // estimate the pairing parameters - if(0 == do_pairing) - { - { // DVK - realignment integration - // run realignment on all matches from records [low]->sams [II] for II from 0 to seqs_buffer[low]->n - // ? should the realignment be run if flowspace realignment is performed ? - yes, if both are requested from command line - if (1 == driver->opt->do_realign) + } + else { - unsigned seqidx, matchidx; - for (seqidx = 0; seqidx < seqs_buffer[low]->n; ++seqidx) + // choose alignments + for (j = 0; j < num_ends; ++j) + { // for each end + tmap_map_sams_filter1 (records [low]->sams [j], stage->opt->aln_output_mode, TMAP_MAP_ALGO_NONE, rand); + stage_stat->num_after_filter += records [low]->sams [j]->n; + } + } + + // generate and post-process alignments + found = 0; + for (j = 0; j < num_ends; ++j) // for each end + { + tmap_map_sams_t* sams = records [low]->sams [j]; + tmap_seq_t* seq = seqs_buffer [low]->seqs [j]; + + //if (0 == strcmp (seq->data.sam->name->s, "K0BK3:04849:06835")) + // tmap_progress_print2 ("here"); + tmap_seq_t** seq_variants = seqs [j]; + // if there are no mappings, continue + if (!sams->n) + continue; + // find alignment starqts + sams = records [low]->sams [j] = tmap_map_util_find_align_starts + ( + index->refseq, // reference server + sams, // initial rough mapping + seq, // read + seq_variants, // array of size 4 that contains pre-computed inverse / complement combinations + stage->opt, // stage parameters + &target, // target cache control structure + stat + ); + if (!sams->n) + continue; + // reference alignment + tmap_map_util_align + ( + index->refseq, // reference server + sams, // mappings to compute alignments for + seq_variants, // array of size 4 that contains pre-computed inverse / complement combinations + &target, // target cache control structure + &path_buf, // buffer for traceback path + &path_buf_sz, // used portion and allocated size of traceback path. + &sw_par, // Smith-Waterman scoring parameters + stat + ); + // realign in flowspace or with context, if requested + if (driver->opt->aln_flowspace) + // NB: seqs_buffer should have its key sequence if 0 < key_seq_len + tmap_map_util_fsw + ( + seq, + sams, + index->refseq, + driver->opt->bw, + driver->opt->softclip_type, + driver->opt->score_thr, + driver->opt->score_match, + driver->opt->pen_mm, + driver->opt->pen_gapo, + driver->opt->pen_gape, + driver->opt->fscore, + 1 - driver->opt->ignore_flowgram, + stat + ); + else if (driver->opt->do_hp_weight) + context_align_read + ( + seq, + index->refseq, + sams, + context, + stat, + driver->opt->log_text_als + ); + // perform anti-dyslexic realignment if enabled + if (driver->opt->do_realign) { - tmap_map_sams_t* sams = records[low]->sams[seqidx]; - tmap_seq_t* qryseq = seqs_buffer[low]->seqs[seqidx]; - tmap_refseq_t* refseq = index->refseq; - - // extract query - const char* qryname = tmap_seq_get_name (qryseq)->s; - tmap_string_t* qrybases = tmap_seq_get_bases (qryseq); - const char* qry = qrybases->s; - for (matchidx = 0; matchidx < sams->n; ++matchidx) + realign_read + ( + seq, + index->refseq, + sams, + realigner, + stat, + driver->opt->log_text_als + ); + } + // salvage ends + if (0 < driver->opt->pen_gapl) + tmap_map_util_salvage_edge_indels + ( + index->refseq, // reference server + sams, // mappings to compute alignments for + seq_variants, // array of size 4 that contains pre-computed inverse / complement combinations + stage->opt, // tmap parameters + &sw_par, // Smith-Waterman scoring parameters + &target, // target cache control structure + &path_buf, // buffer for traceback path + &path_buf_sz, // used portion and allocated size of traceback path. + stat + ); + // trim key + if (1 == stage->opt->softclip_key) + tmap_map_util_trim_key + ( + sams, // mappings to compute alignments for + seq, // read + seq_variants, // array of size 4 that contains pre-computed inverse / complement combinations + index->refseq, // reference server + &target, // target cache control structure + stat + ); + // end repair + if (stage->opt->end_repair) + tmap_map_util_end_repair_bulk + ( + index->refseq, // reference server + sams, // mappings to compute alignments for + seq, // read + seq_variants, // array of size 4 that contains pre-computed inverse / complement combinations + stage->opt, // tmap parameters + &target, // target cache control structure + &path_buf, // buffer for traceback path + &path_buf_sz, // used portion and allocated size of traceback path. + stat + ); + // explicitly fix 5' softclip is required + if (!stage->opt->end_repair_5_prime_softclip && (stage->opt->softclip_type == 2 || stage->opt->softclip_type == 3)) + { + int i; + for (i = 0; i != sams->n; ++i) { - // extract packed cigar and it's length - uint32_t* orig_cigar = sams->sams [matchidx].cigar; - unsigned orig_cigar_sz = sams->sams [matchidx].n_cigar; - // extract seqid - unsigned ref_id = sams->sams [matchidx].seqid; - // extract offset - unsigned ref_off = sams->sams [matchidx].pos; - // extract read direction in alignment - uint8_t forward = (sams->sams [matchidx].strand == 0) ? 1 : 0; - // make inverse/complement copy of the query if direction is reverse - if (!forward) - { - char* qry_rev = qry_mem (realigner, qrybases->l+1); - memcpy (qry_rev, qry, qrybases->l); - qry_rev [qrybases->l] = 0; - tmap_reverse_compliment (qry_rev, (int32_t) qrybases->l); - qry = qry_rev; - } - // compute alignment and subject lengths - unsigned q_len_cigar, r_len_cigar; - seq_lens_from_bin_cigar (orig_cigar, orig_cigar_sz, &q_len_cigar, &r_len_cigar); - uint8_t* ref = (uint8_t*) ref_mem (realigner, r_len_cigar); - int32_t converted_cnt; - // extract reference. This returns reference sequence in UNPACKED but BINARY CONVERTED form - values of 0-4, one byte per base! - tmap_refseq_subseq2 (refseq, ref_id+1, ref_off+1, ref_off + r_len_cigar, ref, 0, &converted_cnt); - // convert reference to ascii format - { - int ii; - for(ii = 0; ii < r_len_cigar; ++ii) - { - if (ref [ii] >= sizeof (tmap_iupac_int_to_char)) - tmap_bug (); - ref [ii] = tmap_iupac_int_to_char [ref [ii]]; - } - ref [r_len_cigar] = 0; - } - // get name for reporting - const char* ref_name = refseq->annos [ref_id].name->s; - // prepare variables to hold results - uint32_t* cigar_dest; - unsigned cigar_dest_sz; - int new_offset; - ++(stat->num_realign_invocations); - uint64_t apo = stat->num_realign_already_perfect; - uint64_t nco = stat->num_realign_not_clipped; - uint64_t swfo = stat->num_realign_sw_failures; - uint64_t ucfo = stat->num_realign_unclip_failures; - const char* al_proc_class = "UNKNOWN"; - // compute the alignment - uint8_t al_mod = 0; - if (realigner_compute_alignment (realigner, - qry, - qrybases->l, - (const char*) ref, - r_len_cigar, - ref_off, - forward, - orig_cigar, - orig_cigar_sz, - &cigar_dest, - &cigar_dest_sz, - &new_offset, - &(stat->num_realign_already_perfect), - &(stat->num_realign_not_clipped), - &(stat->num_realign_sw_failures), - &(stat->num_realign_unclip_failures))) - { - // check if changes were introduced - if (new_offset != ref_off || orig_cigar_sz != cigar_dest_sz || memcmp (orig_cigar, cigar_dest, orig_cigar_sz*sizeof(*orig_cigar))) - { - ++(stat->num_realign_changed); - if (new_offset != ref_off) - { - // log shifted alignment - ++(stat->num_realign_shifted); - if (nco == stat->num_realign_not_clipped) - al_proc_class = "MOD-SHIFT"; - else - al_proc_class = "MOD-SHIFT NOCLIP"; - } - else - { - // log changed alignment - if (nco == stat->num_realign_not_clipped) - al_proc_class = "MOD"; - else - al_proc_class = "MOD NOCLIP"; - } - al_mod = 1; - // pack the alignment back into the originating structure (unallocate memory taken by the old one) - // TODO implement more intelligent alignment memory management, avoid heap operations when possible - free (sams->sams [matchidx].cigar); - sams->sams [matchidx].cigar = cigar_dest; - sams->sams [matchidx].n_cigar = cigar_dest_sz; - sams->sams [matchidx].pos = new_offset; - } - else - { - // log unchanged alignment - free (cigar_dest); - if (nco == stat->num_realign_not_clipped) - al_proc_class = "UNMOD"; - else - al_proc_class = "UNMOD NOCLIP"; - } - } - else - { - // log reason why alignment was not processed - if (swfo != stat->num_realign_sw_failures) - al_proc_class = "SWERR"; - else if (ucfo != stat->num_realign_unclip_failures) - al_proc_class = "UNCLIPERR"; - else if (apo != stat->num_realign_already_perfect) - al_proc_class = "PERFECT"; - } - // log - tmap_log ("REALIGN: %s(%s) vs %s:%d %s. ", qryname, (forward ? "FWD":"REV"), ref_name, ref_off, al_proc_class); - if (al_mod) - { - tmap_log ("orig (%d op) at %d: ", orig_cigar_sz, ref_off); - cigar_log (orig_cigar, orig_cigar_sz); - tmap_log ("; new (%d op) at %d: ", cigar_dest_sz, new_offset); - cigar_log (cigar_dest, cigar_dest_sz); - - if (driver->opt->log_text_als) - { - const unsigned MAX_BATCHNO = 100; - AlBatch batches [MAX_BATCHNO]; - uint32_t q_clip = 0; - int bno = cigar_to_batches (orig_cigar, orig_cigar_sz, &q_clip, batches, MAX_BATCHNO); - // (const char* xseq, unsigned xlen, bool xrev, const char* yseq, unsigned ylen, bool yrev, const AlBatch *b_ptr, int b_cnt, unsigned xoff, unsigned yoff, unsigned margin = 0, unsigned width = 120, bool zero_based = 1) - tmap_log ("\n Before realignment:\n"); - log_batches (qry+q_clip, qrybases->l-q_clip, !forward, (const char*) ref, r_len_cigar, 0, batches, bno, q_clip, ref_off); - - uint32_t q_len_dest, r_len_dest; - seq_lens_from_bin_cigar (cigar_dest, cigar_dest_sz, &q_len_dest, &r_len_dest); - q_clip = 0; - bno = cigar_to_batches (cigar_dest, cigar_dest_sz, &q_clip, batches, MAX_BATCHNO); - // (const char* xseq, unsigned xlen, bool xrev, const char* yseq, unsigned ylen, bool yrev, const AlBatch *b_ptr, int b_cnt, unsigned xoff, unsigned yoff, unsigned margin = 0, unsigned width = 120, bool zero_based = 1) - tmap_log (" After realignment:\n"); - log_batches (qry+q_clip, qrybases->l-q_clip, !forward, (const char*) ref, r_len_dest, 0, batches, bno, q_clip, new_offset); - } - } - tmap_log ("\n"); + tmap_map_util_remove_5_prime_softclip + ( + index->refseq, + sams->sams + i, + seq, + seq_variants, + &target, + &path_buf, + &path_buf_sz, + &sw_par, + stage->opt, + stat + ); } } - } - } // end realignment integration - { // DVK - context-specific realignment integration - // run realignment on all matches from records [low]->sams [II] for II from 0 to seqs_buffer[low]->n - if (1 == driver->opt->do_hp_weight) - { - unsigned seqidx, matchidx; - for (seqidx = 0; seqidx < seqs_buffer[low]->n; ++seqidx) + if (stage->opt->cigar_sanity_check) // do this before tail repeat clipping as the latter does not update alignment box. TODO: update box in tail clip and move this to the very end of alignment post processing { - tmap_map_sams_t* sams = records[low]->sams[seqidx]; - tmap_seq_t* qryseq = seqs_buffer[low]->seqs[seqidx]; - tmap_refseq_t* refseq = index->refseq; - // extract query - const char* qryname = tmap_seq_get_name (qryseq)->s; - - tmap_string_t* qrybases = tmap_seq_get_bases (qryseq); - const char* qry = qrybases->s; - for (matchidx = 0; matchidx < sams->n; ++matchidx) + int i; + for (i = 0; i != sams->n; ++i) { - // extract packed cigar and it's length - uint32_t* orig_cigar = sams->sams [matchidx].cigar; - unsigned orig_cigar_sz = sams->sams [matchidx].n_cigar; - // extract seqid - unsigned ref_id = sams->sams [matchidx].seqid; - // extract offset - unsigned ref_off = sams->sams [matchidx].pos; - // extract read direction in alignment - uint8_t forward = (sams->sams [matchidx].strand == 0) ? 1 : 0; - // make inverse/complement copy of the query if direction is reverse - if (!forward) - { - char* qry_rev = qry_mem (realigner, qrybases->l+1); - memcpy (qry_rev, qry, qrybases->l); - qry_rev [qrybases->l] = 0; - tmap_reverse_compliment (qry_rev, (int32_t) qrybases->l); - qry = qry_rev; - } - // compute alignment and subject lengths - unsigned q_len_cigar, r_len_cigar; - seq_lens_from_bin_cigar (orig_cigar, orig_cigar_sz, &q_len_cigar, &r_len_cigar); - uint8_t* ref = (uint8_t*) ref_mem (realigner, r_len_cigar+1); - int32_t converted_cnt; - // extract reference. This returns reference sequence in UNPACKED but BINARY CONVERTED form - values of 0-4, one byte per base! - tmap_refseq_subseq2 (refseq, ref_id+1, ref_off+1, ref_off + r_len_cigar, ref, 0, &converted_cnt); - // convert reference to ascii format - { - int ii; - for(ii = 0; ii < r_len_cigar; ++ii) - { - if (ref [ii] >= sizeof (tmap_iupac_int_to_char)) - tmap_bug (); - ref [ii] = tmap_iupac_int_to_char [ref [ii]]; - } - ref [r_len_cigar] = 0; - } - const char* ref_name = refseq->annos [ref_id].name->s; - // prepare variables to hold results - uint32_t* cigar_dest; - unsigned cigar_dest_sz; - int new_offset; - ++(stat->num_hpcost_invocations); - // compute the alignment - - if (realigner_compute_alignment (context, - qry, - qrybases->l, - (const char*) ref, - r_len_cigar, - ref_off, - forward, - orig_cigar, - orig_cigar_sz, - &cigar_dest, - &cigar_dest_sz, - &new_offset, - NULL, - NULL, - NULL, - NULL)) - { - // check if changes were introduced - if (new_offset != ref_off || orig_cigar_sz != cigar_dest_sz || memcmp (orig_cigar, cigar_dest, orig_cigar_sz*sizeof (*orig_cigar))) - { - if (tmap_log_enabled ()) - { - tmap_log ("CONTEXT-GAP: %s(%s) vs %s:%d MODIFIED: ", qryname, (forward ? "FWD":"REV"), ref_name, ref_off); - tmap_log ("orig (%d op) at %d: ", orig_cigar_sz, ref_off); - cigar_log (orig_cigar, orig_cigar_sz); - tmap_log ("; new (%d op) at %d: ", cigar_dest_sz, new_offset); - cigar_log (cigar_dest, cigar_dest_sz); - tmap_log ("\n"); - - if (driver->opt->log_text_als) - { - const unsigned MAX_BATCHNO = 100; - AlBatch batches [MAX_BATCHNO]; - uint32_t q_clip = 0; - int bno = cigar_to_batches (orig_cigar, orig_cigar_sz, &q_clip, batches, MAX_BATCHNO); - // (const char* xseq, unsigned xlen, bool xrev, const char* yseq, unsigned ylen, bool yrev, const AlBatch *b_ptr, int b_cnt, unsigned xoff, unsigned yoff, unsigned margin = 0, unsigned width = 120, bool zero_based = 1) - tmap_log (" Before context realignment:\n"); - log_batches (qry+q_clip, qrybases->l-q_clip, !forward, (const char*) ref, r_len_cigar, 0, batches, bno, q_clip, ref_off); - - - uint32_t q_len_dest, r_len_dest; - seq_lens_from_bin_cigar (cigar_dest, cigar_dest_sz, &q_len_dest, &r_len_dest); - q_clip = 0; - bno = cigar_to_batches (cigar_dest, cigar_dest_sz, &q_clip, batches, MAX_BATCHNO); - // (const char* xseq, unsigned xlen, bool xrev, const char* yseq, unsigned ylen, bool yrev, const AlBatch *b_ptr, int b_cnt, unsigned xoff, unsigned yoff, unsigned margin = 0, unsigned width = 120, bool zero_based = 1) - tmap_log (" After context realignment:\n"); - log_batches (qry+q_clip, qrybases->l-q_clip, !forward, (const char*) ref, r_len_dest, 0, batches, bno, q_clip, new_offset); - } - } - - ++(stat->num_hpcost_modified); - if (new_offset != ref_off) - // log shifted alignment - ++(stat->num_hpcost_shifted); - // pack the alignment back into the originating structure (unallocate memory taken by the old one) - if (orig_cigar_sz < cigar_dest_sz) - { - free (sams->sams [matchidx].cigar); - sams->sams [matchidx].cigar = (uint32_t*) tmap_malloc (sizeof (uint32_t) * cigar_dest_sz, "cigar_dest"); - } - sams->sams [matchidx].n_cigar = cigar_dest_sz; - memcpy (sams->sams [matchidx].cigar, cigar_dest, sizeof (uint32_t) * cigar_dest_sz); - sams->sams [matchidx].pos = new_offset; - } - else - tmap_log ("CONTEXT-GAP: %s(%s) vs %s:%d UNCHANGED\n", qryname, (forward ? "FWD":"REV"), ref_name, ref_off); - } - else - { - ++(stat->num_hpcost_skipped); - tmap_log ("CONTEXT-GAP: %s(%s) vs %s:%d SKIPPED\n", qryname, (forward ? "FWD":"REV"), ref_name, ref_off); - } + cigar_sanity_check + ( + index->refseq, + sams->sams + i, + seq, + seq_variants, + &target, + stage->opt + ); } } + // clip repeats + if (driver->opt->do_repeat_clip) + tail_repeats_clip_read + ( + seq, + index->refseq, + sams, + realigner, + stat, + driver->opt->repclip_continuation + ); + + stage_stat->num_with_mapping++; + found = 1; } - } // end context-specific realignment integration - { // DVK soft-clipping tail tandem repeats - if (1 == driver->opt->do_repeat_clip) - { - const unsigned MAX_PERIOD = 10; + // TODO + // if paired, update pairing score based on target start? - // detect the repeat (using naive NxM algorithm) - unsigned seqidx, matchidx; - for (seqidx = 0; seqidx < seqs_buffer[low]->n; ++seqidx) - { - tmap_map_sams_t* sams = records[low]->sams[seqidx]; - tmap_seq_t* qryseq = seqs_buffer[low]->seqs[seqidx]; - tmap_refseq_t* refseq = index->refseq; - - // extract query - const char* qryname = tmap_seq_get_name (qryseq)->s; - tmap_string_t* qrybases = tmap_seq_get_bases (qryseq); - const char* qry = qrybases->s; - unsigned filtered = 0; // some of the alignments could be completely skipped + // did we find any mappings? + if (found) + { // yes + if (stat) + tmap_map_stats_add (stat, stage_stat); + tmap_map_stats_destroy (stage_stat); + break; + } + else + { // no + tmap_map_record_destroy (records [low]); + // re-init + records [low] = tmap_map_record_init (num_ends); + } + tmap_map_stats_destroy (stage_stat); + } - for (matchidx = 0; matchidx < sams->n; ++matchidx) - { - // if necessary, prepare slot for filling in the sam - if (filtered != matchidx) - { - // if we had rejected some of matches, the tail should shrink. (avoid reallocation, just swap all internal pointers. Otherwise it'll be too heavy, and it'll be better to skip them during BAM writing, which is logically more cumbersome) - //fprintf (stderr, "filtered == matchidx! This should not happen while bugcheck is in effect!\n"); - //tmap_bug (); - tmap_map_sam_t tmp = sams->sams [filtered]; - sams->sams [filtered] = sams->sams [matchidx]; - sams->sams [matchidx] = tmp; - } - // extract packed cigar and it's length - uint32_t* orig_cigar = sams->sams [filtered].cigar; - unsigned orig_cigar_sz = sams->sams [filtered].n_cigar; - // extract seqid - unsigned ref_id = sams->sams [filtered].seqid; - // extract offset - unsigned ref_off = sams->sams [filtered].pos; - // extract read direction in alignment - uint8_t forward = (sams->sams [filtered].strand == 0) ? 1 : 0; - // make inverse/complement copy of the query if direction is reverse - // FIX: NO REVERSING OF QUERY! this was a wrong logic. The read should stay as is, - /// but the reference should be extracted for the ACTUAL tail of the read, - // which in case of reverse match located at the mapped location + lenght_of_5'_soft_clip - /* - if (!forward) - { - char* qry_rev = qry_mem (realigner, qrybases->l+1); - memcpy (qry_rev, qry, qrybases->l); - qry_rev [qrybases->l] = 0; - tmap_reverse_compliment (qry_rev, (int32_t) qrybases->l); - qry = qry_rev; - } - */ - // compute alignment and subject lengths - unsigned q_al_beg, q_al_end, r_al_beg, r_al_end; - alignment_bounds_from_bin_cigar (orig_cigar, orig_cigar_sz, forward, qrybases->l, &q_al_beg, &q_al_end, &r_al_beg, &r_al_end); - // check if this read had hit the adapter (even one-base hit makes the repeat clipping invalid) - if (tmap_sam_get_zb (qryseq->data.sam) > 0 && tmap_sam_get_za (qryseq->data.sam) <= q_al_end) - { - filtered ++; - continue; - } - const char* ref_name = refseq->annos [ref_id].name->s; - unsigned qry_rep_off; - if (driver->opt->repclip_continuation) - { - // extract reference 'tail' (continuation). This returns reference sequence in UNPACKED but BINARY CONVERTED form - values of 0-4, one byte per base! - int32_t ref_tail_beg = forward ? (ref_off + r_al_end) : ((MAX_PERIOD < ref_off + r_al_beg) ? (ref_off + r_al_beg - MAX_PERIOD) : 0); - int32_t ref_tail_end = forward ? mymin (refseq->annos [ref_id].len, ref_tail_beg + MAX_PERIOD) : (ref_off + r_al_beg); //inclusive 1-based - same as exclusive 0-based - int32_t ref_tail_len = ref_tail_end - ref_tail_beg; - if (!ref_tail_len) - { - filtered ++; - continue; - } - uint8_t* ref = (uint8_t*) ref_mem (realigner, ref_tail_len); - int32_t converted_cnt; - tmap_refseq_subseq2 (refseq, ref_id+1, ref_tail_beg+1, ref_tail_end, ref, 0, &converted_cnt); - // convert reference to ascii format - { - int ii; - for(ii = 0; ii < ref_tail_len; ++ii) - { - if (ref [ii] >= sizeof (tmap_iupac_int_to_char)) - { - fprintf (stderr, "Invalid base: %d at position %d in reference %s:%d-%d; query is %d, match is %u\n", ref [ii], ii, ref_name, ref_tail_beg+1, ref_tail_end, low, matchidx); - tmap_bug (); - } - ref [ii] = tmap_iupac_int_to_char [ref [ii]]; - } - ref [ref_tail_len] = 0; - } - // inverse/complement for reverse match - if (!forward) - tmap_reverse_compliment ((char*) ref, ref_tail_len); - // check if there is a tail periodicity extending by at least one period into reference overhang; - // tailrep returns 0 if entire (unclipped) alignment gets trimmed - qry_rep_off = tailrep_cont (ref_tail_len, (char*) ref, q_al_end, qry); - } - else - { - qry_rep_off = tailrep (q_al_end, qry, MAX_PERIOD); - } - // qry_rep_off is where ON THE READ (in read's forward direction) the clip should start. - ++ stat->num_seen_tailclipped; - stat->bases_seen_tailclipped += q_al_end; - // soft-clip longest one, if any - if (qry_rep_off != q_al_end) - { - // save original cigar for reporting - uint32_t* orig_cigar = alloca (sizeof (uint32_t) * orig_cigar_sz); - memcpy (orig_cigar, sams->sams [filtered].cigar, sizeof (uint32_t) * orig_cigar_sz); - unsigned ref_rep_off = 0; - unsigned trimmed_cigar_sz = trim_cigar (&(sams->sams [filtered].cigar), orig_cigar_sz, qrybases->l, qry_rep_off, forward, &ref_rep_off); - if (trimmed_cigar_sz) - { - sams->sams [filtered].n_cigar = trimmed_cigar_sz; - if (!forward) - // sams->sams [filtered].pos += q_al_end - qry_rep_off; - BUG (TS-11736): query may be imperfectly aligned to ref in repeat zone, causing wrong coords - sams->sams [filtered].pos += ref_rep_off; - - tmap_log ("TAIL_REP_TRIM: %s(%s) vs %s:%d TRIMMED %d bases. ", qryname, (forward ? "FWD":"REV"), ref_name, ref_off, q_al_end - qry_rep_off); - tmap_log ("orig (%d op) at %d: ", orig_cigar_sz, ref_off); - cigar_log (orig_cigar, orig_cigar_sz); - tmap_log ("; new (%d op) at %d: ", trimmed_cigar_sz, sams->sams [filtered].pos); - cigar_log (sams->sams [filtered].cigar, trimmed_cigar_sz); - tmap_log ("\n"); - - stat->bases_tailclipped += q_al_end - qry_rep_off; // with respect to the read - filtered ++; - } - else - { - tmap_log ("TAIL_REP_TRIM: %s(%s) vs %s:%d REMOVED (FULLY TRIMMED)\n", qryname, (forward ? "FWD":"REV"), ref_name, ref_off); - stat->bases_tailclipped += q_al_end; - stat->bases_fully_tailclipped += q_al_end; - ++ stat->num_fully_tailclipped; - // do not increment filtered; skip this - } - ++ stat->num_tailclipped; - } - else - { - tmap_log ("TAIL_REP_TRIM: %s(%s) vs %s:%d UNCHANGED\n", qryname, (forward ? "FWD":"REV"), ref_name, ref_off); - ++ filtered; - } - } // end alignment processing - sams->n = filtered; - } // end read processing - } // endif driver->opt->do_repeat_clip - } // end tail repeat clipping scope - - // convert the record to bam - if (1 == seqs_buffer [low]->n) - { - bams [low] = tmap_map_bams_init (1); - bams [low]->bams [0] = tmap_map_sams_print (seqs_buffer [low]->seqs [0], index->refseq, records [low]->sams [0], + // only convert to BAM and destroy the records if we are not trying to + // estimate the pairing parameters + if (0 == do_pairing) + { + // convert the record to bam + if (1 == seqs_buffer [low]->n) + { + bams [low] = tmap_map_bams_init (1); + bams [low]->bams [0] = tmap_map_sams_print (seqs_buffer [low]->seqs [0], index->refseq, records [low]->sams [0], 0, NULL, driver->opt->sam_flowspace_tags, driver->opt->bidirectional, driver->opt->seq_eq, driver->opt->min_al_len, driver->opt->min_al_cov, driver->opt->min_identity, driver->opt->score_match, &(stat->num_filtered_als)); - } - else - { - bams [low] = tmap_map_bams_init (seqs_buffer [low]->n); - for(j = 0;j < seqs_buffer [low]->n; j++) - { - bams [low]->bams [j] = tmap_map_sams_print(seqs_buffer [low]->seqs [j], index->refseq, records [low]->sams [j], + } + else + { + bams [low] = tmap_map_bams_init (seqs_buffer [low]->n); + for (j = 0; j < seqs_buffer [low]->n; j++) + bams [low]->bams [j] = tmap_map_sams_print (seqs_buffer [low]->seqs [j], index->refseq, records [low]->sams [j], (0 == j) ? 1 : ((seqs_buffer [low]->n-1 == j) ? 2 : 0), records [low]->sams[(j+1) % seqs_buffer [low]->n], driver->opt->sam_flowspace_tags, driver->opt->bidirectional, driver->opt->seq_eq, driver->opt->min_al_len, driver->opt->min_al_cov, driver->opt->min_identity, driver->opt->score_match, &(stat->num_filtered_als)); - } - } - // free alignments, for space - tmap_map_record_destroy (records [low]); - records [low] = NULL; - } - // free seqs - for (i = 0; i < num_ends; i++) - { - for(j = 0; j < 4; j++) - { - tmap_seq_destroy (seqs [i][j]); - seqs [i][j] = NULL; - } - } - tmap_map_record_destroy (record_prev); - } - // next - (*buffer_idx) = low; - low++; - } - (*buffer_idx) = seqs_buffer_length; - - // free thread variables - for(i=0;iseqs_buffer_length = tmap_seqs_io_read_buffer(d->io_in, d->seqs_buffer, d->reads_queue_size, d->io_out->fp->header->header); + tmap_map_driver_thread_io_data_t *d = (tmap_map_driver_thread_io_data_t*) arg; + d->seqs_buffer_length = tmap_seqs_io_read_buffer (d->io_in, d->seqs_buffer, d->reads_queue_size, d->io_out->fp->header->header); return d; } #endif + + void -tmap_map_driver_core(tmap_map_driver_t *driver) +tmap_map_driver_core (tmap_map_driver_t *driver) { - uint32_t i, j, k, n_reads_processed=0; // # of reads processed - int32_t seqs_buffer_length=0; // # of reads read in - int32_t seqs_loaded=0; // 1 if the seq_buffer is loaded, 0 otherwse + uint32_t i, j, k, n_reads_processed = 0; // # of reads processed + int32_t seqs_buffer_length = 0; // # of reads read in + int32_t seqs_loaded = 0; // 1 if the seq_buffer is loaded, 0 otherwse tmap_seqs_io_t *io_in = NULL; // input file(s) tmap_sam_io_t *io_out = NULL; // output file tmap_seqs_t **seqs_buffer = NULL; // buffer for the reads @@ -1781,11 +1791,10 @@ tmap_map_driver_core(tmap_map_driver_t *driver) #endif time_t start_time = time (NULL); - + if (driver->opt->report_stats) tmap_file_stdout = tmap_file_fdopen(fileno(stdout), "wb", TMAP_FILE_NO_COMPRESSION); - // DVK - realignment #ifdef HAVE_LIBPTHREAD struct RealignProxy** realigner = NULL; @@ -2074,129 +2083,221 @@ tmap_map_driver_core(tmap_map_driver_t *driver) #endif // TODO: should we flush when writing SAM and processing one read at a time? - // print statistics - n_reads_processed += seqs_buffer_length; - if(-1 != driver->opt->reads_queue_size) { - tmap_progress_print2("processed %d reads", n_reads_processed); - tmap_progress_print2("stats [%.2lf,%.2lf,%.2lf,%.2lf,%.2lf,%.2lf]", - stat->num_with_mapping * 100.0 / (double)stat->num_reads, - stat->num_after_seeding/(double)stat->num_with_mapping, - stat->num_after_grouping/(double)stat->num_with_mapping, - stat->num_after_scoring/(double)stat->num_with_mapping, - stat->num_after_rmdup/(double)stat->num_with_mapping, - stat->num_after_filter/(double)stat->num_with_mapping); - if (driver->opt->do_repeat_clip) - { - tmap_progress_print2("total %llu, mapped %llu, clipped %llu, rejected %llu, %.2f%% bases]", + // print statistics + n_reads_processed += seqs_buffer_length; + if (-1 != driver->opt->reads_queue_size) + { + tmap_progress_print2("processed %d reads", n_reads_processed); + tmap_progress_print2("stats [%.2lf,%.2lf,%.2lf,%.2lf,%.2lf,%.2lf]", + stat->num_with_mapping * 100.0 / (double)stat->num_reads, + stat->num_after_seeding/(double)stat->num_with_mapping, + stat->num_after_grouping/(double)stat->num_with_mapping, + stat->num_after_scoring/(double)stat->num_with_mapping, + stat->num_after_rmdup/(double)stat->num_with_mapping, + stat->num_after_filter/(double)stat->num_with_mapping); + if (driver->opt->do_repeat_clip) + { + tmap_progress_print2("total %llu, mapped %llu, clipped %llu, rejected %llu, %.2f%% bases]", stat->num_reads, stat->num_with_mapping, stat->num_tailclipped, stat->num_fully_tailclipped, - ((double) stat->bases_tailclipped) * 100 / stat->bases_seen_tailclipped - ); - } - } - seqs_loaded = 0; - } - if(-1 == driver->opt->reads_queue_size) { - tmap_progress_print2("processed %d reads", n_reads_processed); - tmap_progress_print2("stats [%.2lf,%.2lf,%.2lf,%.2lf,%.2lf,%.2lf]", - stat->num_with_mapping * 100.0 / (double)stat->num_reads, - stat->num_after_seeding/(double)stat->num_with_mapping, - stat->num_after_grouping/(double)stat->num_with_mapping, - stat->num_after_scoring/(double)stat->num_with_mapping, - stat->num_after_rmdup/(double)stat->num_with_mapping, - stat->num_after_filter/(double)stat->num_with_mapping); - } + ((double) stat->bases_tailclipped) * 100 / stat->bases_seen_tailclipped); + } + } + seqs_loaded = 0; + } - // OUTPUT STATS - if (driver->opt->report_stats) - { - time_t end_time = time (NULL); - tmap_file_printf ( "\nMapping completed in %d seconds.\n", end_time-start_time); - tmap_file_printf ( " Total reads: %llu\n", stat->num_reads); - tmap_file_printf ( " Mapped reads: %llu (%.2f%%)\n", stat->num_with_mapping, stat->num_with_mapping * 100.0 / (double)stat->num_reads); - tmap_file_printf ( " After seeding: %llu\n", stat->num_after_seeding); - tmap_file_printf ( " After grouping: %llu\n", stat->num_after_grouping); - tmap_file_printf ( " After scoring: %llu\n", stat->num_after_scoring); - tmap_file_printf ( " After dups removal: %llu\n", stat->num_after_rmdup); - tmap_file_printf ( " After filtering: %llu\n", stat->num_after_filter); - if (!driver->opt->do_realign) - tmap_file_printf ( "No realignment perormed\n"); - else - { - tmap_file_printf ( "Realignment statistics:\n"); - tmap_file_printf ( " Realignment invocations: %llu\n", stat->num_realign_invocations); - tmap_file_printf ( " Not realigned (good): %llu\n"); - if (stat->num_realign_sw_failures) - tmap_file_printf (" Algorithm failures: %llu\n", stat->num_realign_sw_failures); - if (stat->num_realign_unclip_failures) - tmap_file_printf (" Un-clipping failures: %llu\n", stat->num_realign_sw_failures); - tmap_file_printf ( " Altered alignments: %llu", stat->num_realign_changed); - if (stat->num_realign_invocations) - tmap_file_printf ( " (%.2f%% total)", ((double) stat->num_realign_changed) * 100 / stat->num_realign_invocations); - tmap_file_printf ("\n"); - tmap_file_printf ( " Altered positions: %llu", stat->num_realign_shifted); - if (stat->num_realign_shifted) - tmap_file_printf ( " (%.2f%% total, %.2f%% changed)", ((double) stat->num_realign_shifted) * 100 / stat->num_realign_invocations, ((double) stat->num_realign_shifted) * 100 / stat->num_realign_changed); - tmap_file_printf ("\n"); - } - if (!driver->opt->do_hp_weight) - tmap_file_printf ("No realignment with context-dependent gap cost performed\n"); - else - { - tmap_file_printf ( "Context-dependent realignment statistics:\n"); - tmap_file_printf ( " Invocations: %llu\n", stat->num_hpcost_invocations); - tmap_file_printf ( " Skipped (too long): %llu\n", stat->num_hpcost_skipped); - tmap_file_printf ( " Altered alignments: %llu", stat->num_hpcost_modified); - if (stat->num_hpcost_invocations) - tmap_file_printf ( " (%.2f%% total)", ((double) stat->num_hpcost_modified) * 100 / stat->num_hpcost_invocations); - if (stat->num_hpcost_invocations - stat->num_hpcost_skipped) - { - double percent = ((double) stat->num_hpcost_modified * 100) / (stat->num_hpcost_invocations - stat->num_hpcost_skipped); - tmap_file_printf (" (%.2f%% realigned)", percent); - } - tmap_file_printf ("\n"); - tmap_file_printf ( " Altered positions: %llu\n", stat->num_hpcost_shifted); - } - if (!driver->opt->do_repeat_clip) - tmap_file_printf ("No tail repeat clipping performed\n"); - else - { - tmap_file_printf ( " Alignments tail-clipped: %llu", stat->num_tailclipped); - if (stat->num_seen_tailclipped) - tmap_file_printf ( " (%.2f%% seen)", ((double) stat->num_tailclipped) * 100 / stat->num_seen_tailclipped); - tmap_file_printf ("\n"); - tmap_file_printf ( " Tail-clipped bases: %llu", stat->bases_tailclipped); - if (stat->bases_seen_tailclipped) - tmap_file_printf ( " (%.2f%% seen)", ((double) stat->bases_tailclipped) * 100 / stat->bases_seen_tailclipped); - tmap_file_printf ("\n"); - tmap_file_printf ( "Completely clipped reads: %llu", stat->num_fully_tailclipped); - if (stat->num_seen_tailclipped) - tmap_file_printf ( " (%.2f%% clipped)", ((double) stat->num_fully_tailclipped) * 100 / stat->num_tailclipped); - tmap_file_printf (", contain %llu bases", stat->bases_fully_tailclipped); - if (stat->bases_tailclipped) - tmap_file_printf (" (%.2f%% clipped)", ((double) stat->bases_fully_tailclipped) * 100 / stat->bases_tailclipped); - tmap_file_printf ("\n"); - if (stat->num_seen_tailclipped) - { - tmap_file_printf ( " Average bases clipped:\n"); - tmap_file_printf ( " per read: %.1f\n", ((double) stat->bases_tailclipped) / stat->num_seen_tailclipped); - if (stat->num_tailclipped) - tmap_file_printf (" per clipped read: %.1f\n", ((double) stat->bases_tailclipped) / stat->num_tailclipped); - if (stat->num_fully_tailclipped) - tmap_file_printf (" per fully clipped read: %.1f\n", ((double) stat->bases_fully_tailclipped) / stat->num_fully_tailclipped); - } - } - if (!driver->opt->min_al_len && !driver->opt->min_al_cov && !driver->opt->min_identity) - tmap_file_printf ("No alignment filtering performed\n"); - else - { - tmap_file_printf ( " Filtered alignments: %llu\n", stat->num_filtered_als); - } + if(-1 == driver->opt->reads_queue_size) + { + tmap_progress_print2("processed %d reads", n_reads_processed); + tmap_progress_print2("stats [%.2lf,%.2lf,%.2lf,%.2lf,%.2lf,%.2lf]", + stat->num_with_mapping * 100.0 / (double)stat->num_reads, + stat->num_after_seeding/(double)stat->num_with_mapping, + stat->num_after_grouping/(double)stat->num_with_mapping, + stat->num_after_scoring/(double)stat->num_with_mapping, + stat->num_after_rmdup/(double)stat->num_with_mapping, + stat->num_after_filter/(double)stat->num_with_mapping); + } + + // OUTPUT STATS + if (driver->opt->report_stats) + { + time_t end_time = time (NULL); + tmap_file_printf ("\nMapping completed in %d seconds.\n", end_time-start_time); + tmap_file_printf (" Total reads: %llu\n", stat->num_reads); + tmap_file_printf (" Mapped reads: %llu (%.2f%%)\n", stat->num_with_mapping, stat->num_with_mapping * 100.0 / (double)stat->num_reads); + tmap_file_printf (" After seeding: %llu\n", stat->num_after_seeding); + tmap_file_printf (" After grouping: %llu\n", stat->num_after_grouping); + tmap_file_printf (" After scoring: %llu\n", stat->num_after_scoring); + tmap_file_printf (" After dups removal: %llu\n", stat->num_after_rmdup); + tmap_file_printf (" After filtering: %llu\n", stat->num_after_filter); + + if (!driver->opt->do_realign) + tmap_file_printf ( "No realignment perormed\n"); + else + { + tmap_file_printf ("Realignment statistics:\n"); + tmap_file_printf (" Realignment invocations: %llu\n", stat->num_realign_invocations); + tmap_file_printf (" Not realigned (good): %llu\n"); + if (stat->num_realign_sw_failures) + tmap_file_printf (" Algorithm failures: %llu\n", stat->num_realign_sw_failures); + if (stat->num_realign_unclip_failures) + tmap_file_printf (" Un-clipping failures: %llu\n", stat->num_realign_sw_failures); + tmap_file_printf (" Altered alignments: %llu", stat->num_realign_changed); + if (stat->num_realign_invocations) + tmap_file_printf (" (%.2f%% total)", ((double) stat->num_realign_changed) * 100 / stat->num_realign_invocations); + tmap_file_printf ("\n"); + tmap_file_printf (" Altered positions: %llu", stat->num_realign_shifted); + if (stat->num_realign_shifted) + tmap_file_printf (" (%.2f%% total, %.2f%% changed)", ((double) stat->num_realign_shifted) * 100 / stat->num_realign_invocations, ((double) stat->num_realign_shifted) * 100 / stat->num_realign_changed); + tmap_file_printf ("\n"); + } + + if (!driver->opt->do_hp_weight) + tmap_file_printf ("No realignment with context-dependent gap cost performed\n"); + else + { + tmap_file_printf ("Context-dependent realignment statistics:\n"); + tmap_file_printf (" Invocations: %llu\n", stat->num_hpcost_invocations); + tmap_file_printf (" Skipped (too long): %llu\n", stat->num_hpcost_skipped); + tmap_file_printf (" Altered alignments: %llu", stat->num_hpcost_modified); + if (stat->num_hpcost_invocations) + tmap_file_printf (" (%.2f%% total)", ((double) stat->num_hpcost_modified) * 100 / stat->num_hpcost_invocations); + if (stat->num_hpcost_invocations - stat->num_hpcost_skipped) + { + double percent = ((double) stat->num_hpcost_modified * 100) / (stat->num_hpcost_invocations - stat->num_hpcost_skipped); + tmap_file_printf (" (%.2f%% realigned)", percent); + } + tmap_file_printf ("\n"); + tmap_file_printf (" Altered positions: %llu\n", stat->num_hpcost_shifted); + } + + if (!(0 < driver->opt->pen_gapl)) + tmap_file_printf ("No edge long indel salvage performed\n"); + else + { + tmap_file_printf ("Long tail indels salvage : %llu reads\n", stat->reads_salvaged); + tmap_file_printf (" %8s %8s %8s %8s", "5'fwd", "3'fwd", "5'rev", "3'rev\n"); + tmap_file_printf (" Salvaged read ends: %8llu %8llu %8llu %8llu\n", + stat->num_salvaged [F5P], + stat->num_salvaged [F3P], + stat->num_salvaged [R5P], + stat->num_salvaged [R3P]); + tmap_file_printf (" Average query bases: %8.1f %8.1f %8.1f %8.1f\n", + stat->num_salvaged [F5P]?(((double) stat->bases_salvaged_qry [F5P]) / stat->num_salvaged [F5P]):0., + stat->num_salvaged [F3P]?(((double) stat->bases_salvaged_qry [F3P]) / stat->num_salvaged [F3P]):0., + stat->num_salvaged [R5P]?(((double) stat->bases_salvaged_qry [R5P]) / stat->num_salvaged [R5P]):0., + stat->num_salvaged [R3P]?(((double) stat->bases_salvaged_qry [R3P]) / stat->num_salvaged [R3P]):0.); + tmap_file_printf (" Average reference bases: %8.1f %8.1f %8.1f %8.1f\n", + stat->num_salvaged [F5P]?(((double) stat->bases_salvaged_ref [F5P]) / stat->num_salvaged [F5P]):0., + stat->num_salvaged [F3P]?(((double) stat->bases_salvaged_ref [F3P]) / stat->num_salvaged [F3P]):0., + stat->num_salvaged [R5P]?(((double) stat->bases_salvaged_ref [R5P]) / stat->num_salvaged [R5P]):0., + stat->num_salvaged [R3P]?(((double) stat->bases_salvaged_ref [R3P]) / stat->num_salvaged [R3P]):0.); + tmap_file_printf (" Average score change: %8.1f %8.1f %8.1f %8.1f\n", + stat->num_salvaged [F5P]?(((double) stat->score_salvaged_total [F5P]) / stat->num_salvaged [F5P]):0., + stat->num_salvaged [F3P]?(((double) stat->score_salvaged_total [F3P]) / stat->num_salvaged [F3P]):0., + stat->num_salvaged [R5P]?(((double) stat->score_salvaged_total [R5P]) / stat->num_salvaged [R5P]):0., + stat->num_salvaged [R3P]?(((double) stat->score_salvaged_total [R3P]) / stat->num_salvaged [R3P]):0.); + } + + if (!driver->opt->end_repair) + tmap_file_printf ("No end repair performed\n"); + else if (driver->opt->end_repair <= 2) + tmap_file_printf ("\"Old-style\" end repair performed, no statistics collected\n"); + else + { + tmap_file_printf ("End repair : %d reads softclipped, %d extended.\n", stat->reads_end_repair_clipped, stat->reads_end_repair_extended); + tmap_file_printf (" %8s %8s %8s %8s\n", "5'fwd", "3'fwd", "5'rev", "3'rev"); + tmap_file_printf (" Clipped read ends: %8llu %8llu %8llu %8llu\n", + stat->num_end_repair_clipped [F5P], + stat->num_end_repair_clipped [F3P], + stat->num_end_repair_clipped [R5P], + stat->num_end_repair_clipped [R3P]); + tmap_file_printf (" Average bases clipped: %8.1f %8.1f %8.1f %8.1f\n", + stat->num_end_repair_clipped [F5P]?(((double) stat->bases_end_repair_clipped [F5P]) / stat->num_end_repair_clipped [F5P]):0., + stat->num_end_repair_clipped [F3P]?(((double) stat->bases_end_repair_clipped [F3P]) / stat->num_end_repair_clipped [F3P]):0., + stat->num_end_repair_clipped [R5P]?(((double) stat->bases_end_repair_clipped [R5P]) / stat->num_end_repair_clipped [R5P]):0., + stat->num_end_repair_clipped [R3P]?(((double) stat->bases_end_repair_clipped [R3P]) / stat->num_end_repair_clipped [R3P]):0.); + tmap_file_printf (" Extended read ends: %8llu %8llu %8llu %8llu\n", + stat->num_end_repair_extended [F5P], + stat->num_end_repair_extended [F3P], + stat->num_end_repair_extended [R5P], + stat->num_end_repair_extended [R3P]); + tmap_file_printf (" Average bases extended: %8.1f %8.1f %8.1f %8.1f\n", + stat->num_end_repair_extended [F5P]?(((double) stat->bases_end_repair_extended [F5P]) / stat->num_end_repair_extended [F5P]):0., + stat->num_end_repair_extended [F3P]?(((double) stat->bases_end_repair_extended [F3P]) / stat->num_end_repair_extended [F3P]):0., + stat->num_end_repair_extended [R5P]?(((double) stat->bases_end_repair_extended [R5P]) / stat->num_end_repair_extended [R5P]):0., + stat->num_end_repair_extended [R3P]?(((double) stat->bases_end_repair_extended [R3P]) / stat->num_end_repair_extended [R3P]):0.); + tmap_file_printf (" Average indels inserted: %8.1f %8.1f %8.1f %8.1f\n", + stat->num_end_repair_extended [F5P]?(((double) stat->total_end_repair_indel [F5P]) / stat->num_end_repair_extended [F5P]):0., + stat->num_end_repair_extended [F3P]?(((double) stat->total_end_repair_indel [F3P]) / stat->num_end_repair_extended [F3P]):0., + stat->num_end_repair_extended [R5P]?(((double) stat->total_end_repair_indel [R5P]) / stat->num_end_repair_extended [R5P]):0., + stat->num_end_repair_extended [R3P]?(((double) stat->total_end_repair_indel [R3P]) / stat->num_end_repair_extended [R3P]):0.); + } + if (driver->opt->end_repair) + { + if (driver->opt->end_repair_5_prime_softclip) + tmap_file_printf ("5' soft-clipping is allowed for end repair, no softclip removal performed\n"); + else if (driver->opt->softclip_type != 2 && driver->opt->softclip_type != 3) + tmap_file_printf ("5' soft-clipping is explicitly allowed through option \"-g %d\" on command line, no softclip removal performed\n", driver->opt->softclip_type); + else + { + tmap_file_printf ("5' softclip removed on %llu reads\n", stat->num_5_softclips [0] + stat->num_5_softclips [1]); + tmap_file_printf (" %8s %8s\n", "fwd", "rev"); + tmap_file_printf (" Recovered read ends: %8llu %8llu\n", + stat->num_5_softclips [0], + stat->num_5_softclips [1]); + tmap_file_printf (" Average query bases recovered: %8.1f %8.1f\n", + stat->num_5_softclips [0]?(((double) stat->bases_5_softclips_qry [0]) / stat->num_5_softclips [0]):0., + stat->num_5_softclips [1]?(((double) stat->bases_5_softclips_qry [1]) / stat->num_5_softclips [1]):0.); + tmap_file_printf (" Average ref bases recovered: %8.1f %8.1f\n", + stat->num_5_softclips [0]?(((double) stat->bases_5_softclips_ref [0]) / stat->num_5_softclips [0]):0., + stat->num_5_softclips [1]?(((double) stat->bases_5_softclips_ref [1]) / stat->num_5_softclips [1]):0.); + tmap_file_printf (" Average recovered score: %8.1f %8.1f\n", + stat->num_5_softclips [0]?(((double) stat->score_5_softclips_total [0]) / stat->num_5_softclips [0]):0., + stat->num_5_softclips [1]?(((double) stat->score_5_softclips_total [1]) / stat->num_5_softclips [1]):0.); + } + } + + if (!driver->opt->do_repeat_clip) + tmap_file_printf ("No tail repeat clipping performed\n"); + else + { + tmap_file_printf ( " Alignments tail-clipped: %llu", stat->num_tailclipped); + if (stat->num_seen_tailclipped) + tmap_file_printf ( " (%.2f%% seen)", ((double) stat->num_tailclipped) * 100 / stat->num_seen_tailclipped); + tmap_file_printf ("\n"); + tmap_file_printf ( " Tail-clipped bases: %llu", stat->bases_tailclipped); + if (stat->bases_seen_tailclipped) + tmap_file_printf ( " (%.2f%% seen)", ((double) stat->bases_tailclipped) * 100 / stat->bases_seen_tailclipped); + tmap_file_printf ("\n"); + tmap_file_printf ( "Completely clipped reads: %llu", stat->num_fully_tailclipped); + if (stat->num_seen_tailclipped) + tmap_file_printf ( " (%.2f%% clipped)", ((double) stat->num_fully_tailclipped) * 100 / stat->num_tailclipped); + tmap_file_printf (", contain %llu bases", stat->bases_fully_tailclipped); + if (stat->bases_tailclipped) + tmap_file_printf (" (%.2f%% clipped)", ((double) stat->bases_fully_tailclipped) * 100 / stat->bases_tailclipped); + tmap_file_printf ("\n"); + if (stat->num_seen_tailclipped) + { + tmap_file_printf ( " Average bases clipped:\n"); + tmap_file_printf ( " per read: %.1f\n", ((double) stat->bases_tailclipped) / stat->num_seen_tailclipped); + if (stat->num_tailclipped) + tmap_file_printf (" per clipped read: %.1f\n", ((double) stat->bases_tailclipped) / stat->num_tailclipped); + if (stat->num_fully_tailclipped) + tmap_file_printf (" per fully clipped read: %.1f\n", ((double) stat->bases_fully_tailclipped) / stat->num_fully_tailclipped); + } + } + if (!driver->opt->min_al_len && !driver->opt->min_al_cov && !driver->opt->min_identity) + tmap_file_printf ("No alignment filtering performed\n"); + else + { + tmap_file_printf ( " Filtered alignments: %llu\n", stat->num_filtered_als); + } } - - + + tmap_progress_print2("cleaning up"); // cleanup the algorithm persistent data @@ -2237,7 +2338,7 @@ tmap_map_driver_core(tmap_map_driver_t *driver) tmap_log_disable (); if (logfile) fclose (logfile); - + free(records); free(bams); tmap_map_stats_destroy(stat); @@ -2255,7 +2356,7 @@ tmap_map_driver_core(tmap_map_driver_t *driver) #ifdef ENABLE_TMAP_DEBUG_FUNCTIONS tmap_rand_destroy(rand_core); #endif - + // if (driver->opt->report_stats) // tmap_file_fclose (tmap_file_stdout); } diff --git a/Analysis/TMAP/src/map/tmap_map_driver.h b/Analysis/TMAP/src/map/tmap_map_driver.h index 1f34ca20..815d8b30 100644 --- a/Analysis/TMAP/src/map/tmap_map_driver.h +++ b/Analysis/TMAP/src/map/tmap_map_driver.h @@ -31,7 +31,6 @@ typedef int32_t (*tmap_map_driver_func_init)(void **data, tmap_refseq_t *refseq, */ typedef int32_t (*tmap_map_driver_func_thread_init)(void **data, tmap_map_opt_t *opt); - /*! This function will be invoked to map a sequence. @param data the thread persistent data diff --git a/Analysis/TMAP/src/map/util/tmap_map_opt.c b/Analysis/TMAP/src/map/util/tmap_map_opt.c index 95895cec..b50aae88 100644 --- a/Analysis/TMAP/src/map/util/tmap_map_opt.c +++ b/Analysis/TMAP/src/map/util/tmap_map_opt.c @@ -187,6 +187,7 @@ __tmap_map_opt_option_print_func_int_init(max_one_large_indel_rescue) __tmap_map_opt_option_print_func_int_init(min_anchor_large_indel_rescue) __tmap_map_opt_option_print_func_int_init(amplicon_overrun) __tmap_map_opt_option_print_func_int_init(max_adapter_bases_for_soft_clipping) +__tmap_map_opt_option_print_func_tf_init(end_repair_5_prime_softclip) __tmap_map_opt_option_print_func_int_init(shm_key) #ifdef ENABLE_TMAP_DEBUG_FUNCTIONS @@ -212,6 +213,9 @@ __tmap_map_opt_option_print_func_tf_init(do_repeat_clip) // __tmap_map_opt_option_print_func_int_init(repclip_overlap) __tmap_map_opt_option_print_func_tf_init(repclip_continuation) +__tmap_map_opt_option_print_func_int_init(cigar_sanity_check) + + // context-dependent gaps __tmap_map_opt_option_print_func_tf_init(do_hp_weight) __tmap_map_opt_option_print_func_int_init(gap_scale_mode) @@ -454,7 +458,8 @@ tmap_map_opt_options_destroy(tmap_map_opt_options_t *options) static void tmap_map_opt_init_helper(tmap_map_opt_t *opt) { - static char *softclipping_type[] = {"0 - allow on the left and right portion of the read", + static char *softclipping_type[] = { + "0 - allow on the left and right portion of the read", "1 - allow on the left portion of the read", "2 - allow on the right portion of the read", "3 - do not allow soft-clipping", @@ -477,7 +482,8 @@ tmap_map_opt_init_helper(tmap_map_opt_t *opt) "9 - Bladze (Top Coder #6)", "10 - ngthuydiem (Top Coder #7) [Farrar cut-and-paste]", NULL}; - static char *realignment_clip_type[] = {"0 - global realignment", + static char *realignment_clip_type[] = { + "0 - global realignment", "1 - semiglobal (can start/end anywhere in reference)", "2 - semiglobal with soft clip on bead side of a read", "3 - semiglobal with soft clip on key side of a read", @@ -492,6 +498,18 @@ tmap_map_opt_init_helper(tmap_map_opt_t *opt) static char *strandedness[] = {"0 - same strand", "1 - opposite strand", NULL}; static char *positioning[] = {"0 - read one before read two", "1 - read two before read one", NULL}; static char *end_repair[] = {"0 - disable", "1 - prefer mismatches", "2 - prefer indels", ">2 - specify %% Mismatch above which to trim end alignment", NULL}; + static char *sanity_check_outcome [] = { + "0 - do not perform sanity check", + "1 - perform content checks, print warning to stderr; do not check alignment compatibility or scores", + "2 - perform content and alignment compatibility checks, print warnings to stderr; do not check alignment scores", + "3 - perform all checks, print warnings to stderr", + "4 - perform content checks, exit on error", + "5 - perform content checks, exit on error; warn if processed alignment is incompatible with raw one", + "6 - perform content and alignment compatibility checks, exit on error", + "7 - perform content checks, exit on error; warn if processed alignment is incompatible with raw one or if score is suspicious", + "8 - perform content and alignment compatibility checks, exit on error; warn if score is suspicious", + "9 - perform all checks, exit if any of them fails", + NULL}; opt->options = tmap_map_opt_options_init(); @@ -704,7 +722,7 @@ tmap_map_opt_init_helper(tmap_map_opt_t *opt) TMAP_MAP_ALGO_GLOBAL); tmap_map_opt_options_add(opt->options, "end-repair", required_argument, 0, 0 /* no short flag */, TMAP_MAP_OPT_TYPE_INT, - "specifies to perform 5' end repair", + "specifies to perform end repair", end_repair, tmap_map_opt_option_print_func_end_repair, TMAP_MAP_ALGO_GLOBAL); @@ -722,7 +740,7 @@ tmap_map_opt_init_helper(tmap_map_opt_t *opt) TMAP_MAP_ALGO_GLOBAL); tmap_map_opt_options_add(opt->options, "max-amplicon-overrun-large-indel-rescue", required_argument, 0, 0 /* no short flag */, TMAP_MAP_OPT_TYPE_INT, - "the minimum anchor size to rescue with one large indel algorithm", + "the maximum number of bases allowed for a read to overrun the end of amplicon", NULL, tmap_map_opt_option_print_func_amplicon_overrun, TMAP_MAP_ALGO_GLOBAL); @@ -732,6 +750,13 @@ tmap_map_opt_init_helper(tmap_map_opt_t *opt) NULL, tmap_map_opt_option_print_func_max_adapter_bases_for_soft_clipping, TMAP_MAP_ALGO_GLOBAL); + tmap_map_opt_options_add(opt->options, "er-5clip", no_argument, 0, 0, + TMAP_MAP_OPT_TYPE_NONE, + "allow end repair to introduce 5' soft clips", + NULL, + tmap_map_opt_option_print_func_end_repair_5_prime_softclip, + TMAP_MAP_ALGO_GLOBAL); + tmap_map_opt_options_add(opt->options, "shared-memory-key", required_argument, 0, 'k', TMAP_MAP_OPT_TYPE_INT, "use shared memory with the following key", @@ -845,6 +870,16 @@ tmap_map_opt_init_helper(tmap_map_opt_t *opt) tmap_map_opt_option_print_func_repclip_continuation, TMAP_MAP_ALGO_GLOBAL); + + + tmap_map_opt_options_add(opt->options, "cigar-sanity-check", required_argument, 0, 0, + TMAP_MAP_OPT_TYPE_INT, + "perform diagnostics sanity check on all generated alignments", + sanity_check_outcome, + tmap_map_opt_option_print_func_cigar_sanity_check, + TMAP_MAP_ALGO_GLOBAL); + + // context-dependent indel weights tmap_map_opt_options_add(opt->options, "context", no_argument, 0, 0, TMAP_MAP_OPT_TYPE_NONE, @@ -1376,6 +1411,7 @@ tmap_map_opt_init(int32_t algo_id) opt->min_anchor_large_indel_rescue = 6; opt->amplicon_overrun = 6; opt->max_adapter_bases_for_soft_clipping = INT32_MAX; + opt->end_repair_5_prime_softclip = 0; opt->shm_key = 0; opt->min_seq_len = -1; opt->max_seq_len = -1; @@ -1396,6 +1432,9 @@ tmap_map_opt_init(int32_t algo_id) // tail repeat clipping opt->do_repeat_clip = 0; opt->repclip_continuation = 0; + + opt->cigar_sanity_check = TMAP_MAP_SANITY_NONE; + // context dependent gap scores opt->do_hp_weight = 0; opt->gap_scale_mode = TMAP_CONTEXT_GAP_SCALE_GEP; @@ -1904,8 +1943,9 @@ tmap_map_opt_parse(int argc, char *argv[], tmap_map_opt_t *opt) } else if(0 == c && 0 == strcmp("max-amplicon-overrun-large-indel-rescue", options[option_index].name)) { opt->amplicon_overrun = atoi(optarg); } - - + else if (0 == c && 0 == strcmp ("er-5clip", options [option_index].name)) { + opt->end_repair_5_prime_softclip = 1; + } // End of global options // realignment options @@ -1947,6 +1987,9 @@ tmap_map_opt_parse(int argc, char *argv[], tmap_map_opt_t *opt) else if (0 == c && 0 == strcmp ("repclip-cont", options [option_index].name)) { opt->repclip_continuation = 1; } + else if (0 == c && 0 == strcmp ("cigar-sanity-check", options [option_index].name)) { + opt->cigar_sanity_check = atoi (optarg); + } // context-dependent gap scoring else if (0 == c && 0 == strcmp ("context", options [option_index].name)) { opt->do_hp_weight = 1; @@ -2508,6 +2551,7 @@ tmap_map_opt_check(tmap_map_opt_t *opt) tmap_error_cmd_check_int(opt->output_type, 0, 2, "-o"); tmap_error_cmd_check_int(opt->end_repair, 0, 100, "--end-repair"); tmap_error_cmd_check_int(opt->max_adapter_bases_for_soft_clipping, 0, INT32_MAX, "max-adapter-bases-for-soft-clipping"); + tmap_error_cmd_check_int(opt->end_repair_5_prime_softclip, 0, 1, "--er-5clip"); #ifdef ENABLE_TMAP_DEBUG_FUNCTIONS tmap_error_cmd_check_int(opt->sample_reads, 0, 1, "-x"); #endif @@ -2559,6 +2603,8 @@ tmap_map_opt_check(tmap_map_opt_t *opt) tmap_error_cmd_check_int (opt->do_repeat_clip, 0, 1, "--do-repeat-clip"); tmap_error_cmd_check_int (opt->repclip_continuation, 0, 1, "--repclip_cont"); + tmap_error_cmd_check_int (opt->cigar_sanity_check, 0, TMAP_MAP_SANITY_LASTVAL, "--cigar-sanity-check"); + // context dependent gap scores tmap_error_cmd_check_int (opt->do_hp_weight, 0, 1, "--context"); tmap_error_cmd_check_int (opt->gap_scale_mode, 0, 2, "--hpscale"); @@ -2727,12 +2773,15 @@ tmap_map_opt_copy_global(tmap_map_opt_t *opt_dest, tmap_map_opt_t *opt_src) opt_dest->min_anchor_large_indel_rescue = opt_src->min_anchor_large_indel_rescue; opt_dest->amplicon_overrun = opt_src->amplicon_overrun; opt_dest->max_adapter_bases_for_soft_clipping = opt_src->max_adapter_bases_for_soft_clipping; + opt_dest->end_repair_5_prime_softclip = opt_src->end_repair_5_prime_softclip; opt_dest->shm_key = opt_src->shm_key; #ifdef ENABLE_TMAP_DEBUG_FUNCTIONS opt_dest->sample_reads = opt_src->sample_reads; #endif opt_dest->vsw_type = opt_src->vsw_type; - + + opt_dest->cigar_sanity_check = opt_src->cigar_sanity_check; + // flowspace options opt_dest->fscore = opt_src->fscore; opt_dest->softclip_key = opt_src->softclip_key; @@ -2818,6 +2867,7 @@ tmap_map_opt_print(tmap_map_opt_t *opt) fprintf(stderr, "min-anchor-large-indel-rescue=%d\n",opt->min_anchor_large_indel_rescue); fprintf(stderr, "max-amplicon-overrun-indel-rescue=%d", opt->amplicon_overrun); fprintf(stderr, "max_adapter_bases_for_soft_clipping=%d\n", opt->max_adapter_bases_for_soft_clipping); + fprintf(stderr, "end_repair_5_prime_softclip=%d\n", opt->end_repair_5_prime_softclip); fprintf(stderr, "shm_key=%d\n", (int)opt->shm_key); #ifdef ENABLE_TMAP_DEBUG_FUNCTIONS fprintf(stderr, "sample_reads=%lf\n", opt->sample_reads); diff --git a/Analysis/TMAP/src/map/util/tmap_map_opt.h b/Analysis/TMAP/src/map/util/tmap_map_opt.h index cf7fd836..db08b2d6 100644 --- a/Analysis/TMAP/src/map/util/tmap_map_opt.h +++ b/Analysis/TMAP/src/map/util/tmap_map_opt.h @@ -112,6 +112,23 @@ enum { TMAP_CONTEXT_GAP_SCALE_BOTH = 2 }; +/*! + The alignment sanity check modes + */ +enum { + TMAP_MAP_SANITY_NONE = 0, /*!< do not perform sanity check */ + TMAP_MAP_SANITY_WARN_CONTENT = 1, /*!< perform content checks, print warning to stdout; do not check alignment consistency or scores */ + TMAP_MAP_SANITY_WARN_CONTENT_ALIGN = 2, /*!< perform content and alignment compatibility checks, print warning to stdout; do not check alignment scores */ + TMAP_MAP_SANITY_WARN_ALL = 3, /*!< perform all checks, print warnings to stdout */ + TMAP_MAP_SANITY_ERR_CONTENT = 4, /*!< perform content checks, exit on error */ + TMAP_MAP_SANITY_ERR_CONTENT_WARN_ALIGN = 5, /*!< perform content checks, exit on error; warn if processed alignment is incompatible with raw one */ + TMAP_MAP_SANITY_ERR_CONTENT_WARN_ALIGN_SCORE = 6, /*!< perform content checks, exit on error; warn if processed alignment is incompatible with raw one or if score is suspicious */ + TMAP_MAP_SANITY_ERR_CONTENT_ALIGN = 7, /*!< perform content and alignment compatibility checks, exit on error */ + TMAP_MAP_SANITY_ERR_CONTENT_ALIGN_WARN_SCORE = 8, /*!< perform content and alignment compatibility checks, exit on error; warn if score is suspicious */ + TMAP_MAP_SANITY_ERR_ALL = 9, /*!< perform all checks, exit if any of them fails */ + TMAP_MAP_SANITY_LASTVAL = 9 +}; + /*! The various option types */ @@ -218,6 +235,8 @@ typedef struct __tmap_map_opt_t { int32_t min_anchor_large_indel_rescue; /*!< minimum size of anchor needed to open one large gap*/ int32_t amplicon_overrun; /*!< maximum allowed alignment to overrun amplicon edge in one large indel alignment*/ int32_t max_adapter_bases_for_soft_clipping; /*!< specifies to perform 3' soft-clipping (via -g) if at most this # of adapter bases were found (ZB tag) (--max-adapter-bases-for-soft-clipping) */ + int32_t end_repair_5_prime_softclip; /*!< end-repair is allowed to introduce 5' softclip */ + key_t shm_key; /*!< the shared memory key (-k,--shared-memory-key) */ #ifdef ENABLE_TMAP_DEBUG_FUNCTIONS double sample_reads; /*!< sample the reads at this fraction (-x,--sample-reads) */ @@ -241,13 +260,15 @@ typedef struct __tmap_map_opt_t { double context_gip_score; /*!< context realignment gap opening score */ double context_gep_score; /*!< context realignment gap extension score */ int32_t context_extra_bandwidth; /*!< context realignment DP matrix extra band width */ - int32_t context_debug_log; /*!< output detailed log of alignment operation into log */ + int32_t context_debug_log; /*!< output detailed log of context alignment (scoring matrix) into a log file (designated by realign_log)*/ // DVK: tandem repeat end-clipping int32_t do_repeat_clip; /*!< clip tandem repeats at the alignment ends */ // int32_t repclip_overlap; /*! repeat clipping is not performed if read overlaps amplicon end by this number of bases (or more) */ int32_t repclip_continuation; /*! repeat clipping performed only if repeat continues past the end of the read into the reference by at least 1 period */ + int32_t cigar_sanity_check; + // DVK: alignment length filtering int32_t min_al_len; /*!< minimal alignment length to report, -1 to disable */ double min_al_cov; /*!< minimal aligned fraction of the read */ diff --git a/Analysis/TMAP/src/map/util/tmap_map_stats.c b/Analysis/TMAP/src/map/util/tmap_map_stats.c index 4dfdb0a1..4094798b 100644 --- a/Analysis/TMAP/src/map/util/tmap_map_stats.c +++ b/Analysis/TMAP/src/map/util/tmap_map_stats.c @@ -5,6 +5,7 @@ #include #include #include +#include #include "../../util/tmap_error.h" #include "../../util/tmap_alloc.h" #include "../../util/tmap_definitions.h" @@ -13,7 +14,7 @@ tmap_map_stats_t* tmap_map_stats_init() { - tmap_map_stats_t* r = tmap_calloc(1, sizeof(tmap_map_stats_t), "return"); + tmap_map_stats_t* r = tmap_calloc (1, sizeof(tmap_map_stats_t), "stats"); // DVK - guarantee initialization to zero of all members (actually calloc does this anyway) tmap_map_stats_zero (r); @@ -24,7 +25,7 @@ tmap_map_stats_init() void tmap_map_stats_destroy(tmap_map_stats_t *s) { - free(s); + free (s); } void @@ -37,6 +38,7 @@ tmap_map_stats_zero(tmap_map_stats_t *s) void tmap_map_stats_add(tmap_map_stats_t *dest, tmap_map_stats_t *src) { + int i; dest->num_reads += src->num_reads; dest->num_with_mapping += src->num_with_mapping; dest->num_after_seeding += src->num_after_seeding; @@ -45,6 +47,11 @@ tmap_map_stats_add(tmap_map_stats_t *dest, tmap_map_stats_t *src) dest->num_after_rmdup += src->num_after_rmdup; dest->num_after_filter += src->num_after_filter; + dest->num_hpcost_invocations += src->num_hpcost_invocations; + dest->num_hpcost_skipped += src->num_hpcost_skipped; + dest->num_hpcost_modified += src->num_hpcost_modified; + dest->num_hpcost_shifted += src->num_hpcost_shifted; + dest->num_realign_invocations += src->num_realign_invocations; dest->num_realign_already_perfect += src->num_realign_already_perfect; dest->num_realign_not_clipped += src->num_realign_not_clipped; @@ -53,6 +60,25 @@ tmap_map_stats_add(tmap_map_stats_t *dest, tmap_map_stats_t *src) dest->num_realign_changed += src->num_realign_changed; dest->num_realign_shifted += src->num_realign_shifted; + dest->reads_salvaged += src->reads_salvaged; + for (i = 0; i != 4; ++i) dest->num_salvaged [i] += src->num_salvaged [i]; + for (i = 0; i != 4; ++i) dest->bases_salvaged_qry [i] += src->bases_salvaged_qry [i]; + for (i = 0; i != 4; ++i) dest->bases_salvaged_ref [i] += src->bases_salvaged_ref [i]; + for (i = 0; i != 4; ++i) dest->score_salvaged_total [i] += src->score_salvaged_total [i]; + + dest->reads_end_repair_clipped += src->reads_end_repair_clipped; + for (i = 0; i != 4; ++i) dest->num_end_repair_clipped [i] += src->num_end_repair_clipped [i]; + for (i = 0; i != 4; ++i) dest->bases_end_repair_clipped [i] += src->bases_end_repair_clipped [i]; + dest->reads_end_repair_extended += src->reads_end_repair_extended; + for (i = 0; i != 4; ++i) dest->num_end_repair_extended [i] += src->num_end_repair_extended [i]; + for (i = 0; i != 4; ++i) dest->bases_end_repair_extended [i] += src->bases_end_repair_extended [i]; + for (i = 0; i != 4; ++i) dest->total_end_repair_indel [i] += src->total_end_repair_indel [i]; + + for (i = 0; i != 2; ++i) dest->num_5_softclips [i] += src->num_5_softclips [i]; + for (i = 0; i != 2; ++i) dest->bases_5_softclips_qry [i] += src->bases_5_softclips_qry [i]; + for (i = 0; i != 2; ++i) dest->bases_5_softclips_ref [i] += src->bases_5_softclips_ref [i]; + for (i = 0; i != 2; ++i) dest->score_5_softclips_total [i] += src->score_5_softclips_total [i]; + dest->num_seen_tailclipped += src->num_seen_tailclipped; dest->bases_seen_tailclipped += src->bases_seen_tailclipped; dest->num_tailclipped += src->num_tailclipped; @@ -60,45 +86,119 @@ tmap_map_stats_add(tmap_map_stats_t *dest, tmap_map_stats_t *src) dest->num_fully_tailclipped += src->num_fully_tailclipped; dest->bases_fully_tailclipped += src->bases_fully_tailclipped; - dest->num_hpcost_invocations += src->num_hpcost_invocations; - dest->num_hpcost_skipped += src->num_hpcost_skipped; - dest->num_hpcost_modified += src->num_hpcost_modified; - dest->num_hpcost_shifted += src->num_hpcost_shifted; - dest->num_filtered_als += src->num_filtered_als; } void tmap_map_stats_print(tmap_map_stats_t *s) { - fprintf(stderr, "num_reads=%llu\n", (unsigned long long int)s->num_reads); - fprintf(stderr, "num_with_mapping=%llu\n", (unsigned long long int)s->num_with_mapping); - fprintf(stderr, "num_after_seeding=%llu\n", (unsigned long long int)s->num_after_seeding); - fprintf(stderr, "num_after_grouping=%llu\n", (unsigned long long int)s->num_after_grouping); - fprintf(stderr, "num_after_scoring=%llu\n", (unsigned long long int)s->num_after_scoring); - fprintf(stderr, "num_after_rmdup=%llu\n", (unsigned long long int)s->num_after_rmdup); - fprintf(stderr, "num_after_filter=%llu\n", (unsigned long long int)s->num_after_filter); + fprintf (stderr, "num_reads=%llu\n", (unsigned long long int) s->num_reads); + fprintf (stderr, "num_with_mapping=%llu\n", (unsigned long long int) s->num_with_mapping); + fprintf (stderr, "num_after_seeding=%llu\n", (unsigned long long int) s->num_after_seeding); + fprintf (stderr, "num_after_grouping=%llu\n", (unsigned long long int) s->num_after_grouping); + fprintf (stderr, "num_after_scoring=%llu\n", (unsigned long long int) s->num_after_scoring); + fprintf (stderr, "num_after_rmdup=%llu\n", (unsigned long long int) s->num_after_rmdup); + fprintf (stderr, "num_after_filter=%llu\n", (unsigned long long int) s->num_after_filter); + + fprintf (stderr, "num_hpcost_invocations=%llu\n", (unsigned long long int) s->num_hpcost_invocations); + fprintf (stderr, "num_hpcost_skipped=%llu\n", (unsigned long long int) s->num_hpcost_skipped); + fprintf (stderr, "num_hpcost_modified=%llu\n", (unsigned long long int) s->num_hpcost_modified); + fprintf (stderr, "num_hpcost_shifted=%llu\n", (unsigned long long int) s->num_hpcost_shifted); + + fprintf (stderr, "num_realign_invocations=%llu\n", (unsigned long long int) s->num_realign_invocations); + fprintf (stderr, "num_realign_already_perfect=%llu\n", (unsigned long long int) s->num_realign_already_perfect); + fprintf (stderr, "num_realign_not_clipped=%llu\n", (unsigned long long int) s->num_realign_not_clipped); + fprintf (stderr, "num_realign_sw_failures=%llu\n", (unsigned long long int) s->num_realign_sw_failures); + fprintf (stderr, "num_realign_unclip_failures=%llu\n", (unsigned long long int) s->num_realign_unclip_failures); + fprintf (stderr, "num_realign_changed=%llu\n", (unsigned long long int) s->num_realign_changed); + fprintf (stderr, "num_realign_shifted=%llu\n", (unsigned long long int) s->num_realign_shifted); + + + fprintf (stderr, "num_salvaged [reads, fwd5', fwd3', rev5', rev3']=[%llu, %llu, %llu, %llu, %llu]\n", + (unsigned long long int) s->reads_salvaged, + (unsigned long long int) s->num_salvaged [F5P], + (unsigned long long int) s->num_salvaged [F3P], + (unsigned long long int) s->num_salvaged [R5P], + (unsigned long long int) s->num_salvaged [R3P]); + + fprintf (stderr, "bases_salvaged_qry [fwd5', fwd3', rev5', rev3']=[%llu, %llu, %llu, %llu]\n", + (unsigned long long int) s->bases_salvaged_qry [F5P], + (unsigned long long int) s->bases_salvaged_qry [F3P], + (unsigned long long int) s->bases_salvaged_qry [R5P], + (unsigned long long int) s->bases_salvaged_qry [R3P]); + + fprintf (stderr, "bases_salvaged_ref [fwd5', fwd3', rev5', rev3']=[%llu, %llu, %llu, %llu]\n", + (unsigned long long int) s->bases_salvaged_ref [F5P], + (unsigned long long int) s->bases_salvaged_ref [F3P], + (unsigned long long int) s->bases_salvaged_ref [R5P], + (unsigned long long int) s->bases_salvaged_ref [R3P]); + + fprintf (stderr, "score_salvaged_total [fwd5', fwd3', rev5', rev3']=[%lld, %lld, %lld, %lld]\n", + (long long int) s->score_salvaged_total [F5P], + (long long int) s->score_salvaged_total [F3P], + (long long int) s->score_salvaged_total [R5P], + (long long int) s->score_salvaged_total [R3P]); + + fprintf (stderr, "num_end_repair_clipped [reads, fwd5', fwd3', rev5', rev3']=[%llu, %llu, %llu, %llu, %llu]\n", + (unsigned long long int) s->reads_end_repair_clipped, + (unsigned long long int) s->num_end_repair_clipped [F5P], + (unsigned long long int) s->num_end_repair_clipped [F3P], + (unsigned long long int) s->num_end_repair_clipped [R5P], + (unsigned long long int) s->num_end_repair_clipped [R3P]); + + fprintf (stderr, "bases_end_repair_clipped [fwd5', fwd3', rev5', rev3']=[%llu, %llu, %llu, %llu]\n", + (unsigned long long int) s->bases_end_repair_clipped [F5P], + (unsigned long long int) s->bases_end_repair_clipped [F3P], + (unsigned long long int) s->bases_end_repair_clipped [R5P], + (unsigned long long int) s->bases_end_repair_clipped [R3P]); + + fprintf (stderr, "num_end_repair_extended [reads, fwd5', fwd3', rev5', rev3']=[%llu, %llu, %llu, %llu, %llu]\n", + (unsigned long long int) s->reads_end_repair_extended, + (unsigned long long int) s->num_end_repair_extended [F5P], + (unsigned long long int) s->num_end_repair_extended [F3P], + (unsigned long long int) s->num_end_repair_extended [R5P], + (unsigned long long int) s->num_end_repair_extended [R3P]); + + fprintf (stderr, "bases_end_repair_extended [fwd5', fwd3', rev5', rev3']=[%llu, %llu, %llu, %llu]\n", + (unsigned long long int) s->bases_end_repair_extended [F5P], + (unsigned long long int) s->bases_end_repair_extended [F3P], + (unsigned long long int) s->bases_end_repair_extended [R5P], + (unsigned long long int) s->bases_end_repair_extended [R3P]); + + fprintf (stderr, "total_end_repair_indel [fwd5', fwd3', rev5', rev3']=[%llu, %llu, %llu, %llu]\n", + (unsigned long long int) s->total_end_repair_indel [F5P], + (unsigned long long int) s->total_end_repair_indel [F3P], + (unsigned long long int) s->total_end_repair_indel [R5P], + (unsigned long long int) s->total_end_repair_indel [R3P]); + + fprintf (stderr, "num_5_softclips [fwd, rev]=[%llu, %llu]\n", + (unsigned long long int) s->num_5_softclips [0], + (unsigned long long int) s->num_5_softclips [1]); + + fprintf (stderr, "bases_5_softclips_qry [fwd, rev]=[%llu, %llu]\n", + (unsigned long long int) s->bases_5_softclips_qry [0], + (unsigned long long int) s->bases_5_softclips_qry [1]); + + fprintf (stderr, "bases_5_softclips_ref [fwd, rev]=[%llu, %llu]\n", + (unsigned long long int) s->bases_5_softclips_ref [0], + (unsigned long long int) s->bases_5_softclips_ref [1]); - fprintf(stderr, "num_realign_invocations=%llu\n", (unsigned long long int)s->num_realign_invocations); - fprintf(stderr, "num_realign_already_perfect=%llu\n", (unsigned long long int)s->num_realign_already_perfect); - fprintf(stderr, "num_realign_not_clipped=%llu\n", (unsigned long long int)s->num_realign_not_clipped); - fprintf(stderr, "num_realign_sw_failures=%llu\n", (unsigned long long int)s->num_realign_sw_failures); - fprintf(stderr, "num_realign_unclip_failures=%llu\n", (unsigned long long int)s->num_realign_unclip_failures); - fprintf(stderr, "num_realign_changed=%llu\n", (unsigned long long int)s->num_realign_changed); - fprintf(stderr, "num_realign_shifted=%llu\n", (unsigned long long int)s->num_realign_shifted); - - fprintf(stderr, "num_seen_tailclipped=%llu\n", (unsigned long long int)s->num_seen_tailclipped); - fprintf(stderr, "bases_seen_tailclipped=%llu\n", (unsigned long long int)s->bases_seen_tailclipped); - fprintf(stderr, "num_tailclipped=%llu\n", (unsigned long long int)s->num_tailclipped); - fprintf(stderr, "bases_tailclipped=%llu\n", (unsigned long long int)s->bases_tailclipped); - fprintf(stderr, "num_fully_tailclipped=%llu\n", (unsigned long long int)s->num_fully_tailclipped); - fprintf(stderr, "bases_fully_tailclipped=%llu\n", (unsigned long long int)s->bases_fully_tailclipped); + fprintf (stderr, "bases_5_softclips_ref [fwd, rev]=[%llu, %llu]\n", + (unsigned long long int) s->bases_5_softclips_ref [0], + (unsigned long long int) s->bases_5_softclips_ref [1]); + + fprintf (stderr, "score_5_softclips_total [fwd, rev]=[%lld, %lld]\n", + (long long int) s->score_5_softclips_total [0], + (long long int) s->score_5_softclips_total [1]); + + fprintf (stderr, "num_seen_tailclipped=%llu\n", (unsigned long long int)s->num_seen_tailclipped); + fprintf (stderr, "bases_seen_tailclipped=%llu\n", (unsigned long long int)s->bases_seen_tailclipped); + fprintf (stderr, "num_tailclipped=%llu\n", (unsigned long long int)s->num_tailclipped); + fprintf (stderr, "bases_tailclipped=%llu\n", (unsigned long long int)s->bases_tailclipped); + fprintf (stderr, "num_fully_tailclipped=%llu\n", (unsigned long long int)s->num_fully_tailclipped); + fprintf (stderr, "bases_fully_tailclipped=%llu\n", (unsigned long long int)s->bases_fully_tailclipped); - fprintf(stderr, "num_hpcost_invocations=%llu\n", (unsigned long long int)s->num_hpcost_invocations); - fprintf(stderr, "num_hpcost_skipped=%llu\n", (unsigned long long int)s->num_hpcost_skipped); - fprintf(stderr, "num_hpcost_modified=%llu\n", (unsigned long long int)s->num_hpcost_modified); - fprintf(stderr, "num_hpcost_shifted=%llu\n", (unsigned long long int)s->num_hpcost_shifted); - fprintf(stderr, "num_filtered_als=%llu\n", (unsigned long long int)s->num_filtered_als); + fprintf (stderr, "num_filtered_als=%llu\n", (unsigned long long int)s->num_filtered_als); } diff --git a/Analysis/TMAP/src/map/util/tmap_map_stats.h b/Analysis/TMAP/src/map/util/tmap_map_stats.h index b8f8ceb6..2971d1c2 100644 --- a/Analysis/TMAP/src/map/util/tmap_map_stats.h +++ b/Analysis/TMAP/src/map/util/tmap_map_stats.h @@ -5,7 +5,16 @@ /*! The mapping statistics structure. */ -typedef struct { +enum // index into stats arrays +{ + F5P = 0, + F3P = 1, + R5P = 2, + R3P = 3 +}; + +typedef struct +{ uint64_t num_reads; /*!< the number of reads with at least one mapping */ uint64_t num_with_mapping; /*!< the number of reads with at least one mapping */ uint64_t num_after_seeding; /*!< the number of hits after seeding */ @@ -13,6 +22,11 @@ typedef struct { uint64_t num_after_scoring; /*!< the number of hits after scoring */ uint64_t num_after_rmdup; /*!< the number of hits after duplicate removal */ uint64_t num_after_filter; /*!< the number of hits after filtering */ + // statistics for realignment with context-dependent gap cost + uint64_t num_hpcost_invocations; + uint64_t num_hpcost_skipped; + uint64_t num_hpcost_modified; + uint64_t num_hpcost_shifted; // realigner statistics uint64_t num_realign_invocations; /*!< the number of alignments ran through realignment procedure*/ uint64_t num_realign_already_perfect; /*!< the number of alignments that considered already perfect and thus were not re-processed*/ @@ -21,6 +35,25 @@ typedef struct { uint64_t num_realign_unclip_failures; /*!< the number of realigner un-clipping procedure failures*/ uint64_t num_realign_changed; /*!< the number of alignments that were modified adjusted by realigner*/ uint64_t num_realign_shifted; /*!< the number of alignments for which the position on the reference was altered by realigner*/ + // long gap salvage stats + uint64_t reads_salvaged; + uint64_t num_salvaged [4]; + uint64_t bases_salvaged_qry [4]; + uint64_t bases_salvaged_ref [4]; + int64_t score_salvaged_total [4]; + // end repair stats + uint64_t reads_end_repair_clipped; + uint64_t num_end_repair_clipped [4]; + uint64_t bases_end_repair_clipped [4]; + uint64_t reads_end_repair_extended; + uint64_t num_end_repair_extended [4]; + uint64_t bases_end_repair_extended [4]; + uint64_t total_end_repair_indel [4]; + // 5' softclip removal stats + uint64_t num_5_softclips [2]; + uint64_t bases_5_softclips_qry [2]; + uint64_t bases_5_softclips_ref [2]; + int64_t score_5_softclips_total [2]; // tail repeat clipping stats uint64_t num_seen_tailclipped; uint64_t bases_seen_tailclipped; @@ -28,11 +61,6 @@ typedef struct { uint64_t bases_tailclipped; uint64_t num_fully_tailclipped; uint64_t bases_fully_tailclipped; - // statistics for realignment with context-dependent gap cost - uint64_t num_hpcost_invocations; - uint64_t num_hpcost_skipped; - uint64_t num_hpcost_modified; - uint64_t num_hpcost_shifted; // number of filtered alignments uint64_t num_filtered_als; } tmap_map_stats_t; @@ -41,13 +69,13 @@ typedef struct { @return a new stats structure */ tmap_map_stats_t* -tmap_map_stats_init(); +tmap_map_stats_init (); /*! @param s the mapping driver stats to destroy */ void -tmap_map_stats_destroy(tmap_map_stats_t *s); +tmap_map_stats_destroy (tmap_map_stats_t *s); /*! Adds the src stats to the dest stats @@ -55,13 +83,13 @@ tmap_map_stats_destroy(tmap_map_stats_t *s); @param src the source */ void -tmap_map_stats_add(tmap_map_stats_t *dest, tmap_map_stats_t *src); +tmap_map_stats_add (tmap_map_stats_t *dest, tmap_map_stats_t *src); /*! Zeroes all counters @param s the pointer to stats holding object */ void -tmap_map_stats_zero(tmap_map_stats_t *s); +tmap_map_stats_zero (tmap_map_stats_t *s); #endif // TMAP_MAP_STATS_H diff --git a/Analysis/TMAP/src/map/util/tmap_map_util.c b/Analysis/TMAP/src/map/util/tmap_map_util.c index 58ae2fdf..c86c4961 100644 --- a/Analysis/TMAP/src/map/util/tmap_map_util.c +++ b/Analysis/TMAP/src/map/util/tmap_map_util.c @@ -1,7 +1,9 @@ /* Copyright (C) 2010 Ion Torrent Systems, Inc. All Rights Reserved */ + #include #include #include +#include #include "../../util/tmap_alloc.h" #include "../../util/tmap_error.h" #include "../../util/tmap_sam_convert.h" @@ -29,6 +31,9 @@ _query[_ql-_i-1] = _tmp; \ } + +// #define WARN_END_REPAIR_FAILURE 1 + // sort by strand, min-seqid, min-position #define __tmap_map_sam_sort_coord_lt(a, b) ( ((a).strand < (b).strand) \ || ( (a).strand == (b).strand && (a).seqid < (b).seqid) \ @@ -59,6 +64,23 @@ TMAP_SORT_INIT(tmap_map_sam_sort_coord_end, tmap_map_sam_t, __tmap_map_sam_sort_ TMAP_SORT_INIT(tmap_map_sam_sort_coord_score, tmap_map_sam_t, __tmap_map_sam_sort_coord_score_lt) TMAP_SORT_INIT(tmap_map_sam_sort_score_coord, tmap_map_sam_t, __tmap_map_sam_sort_score_coord_lt) +static void tmap_map_util_set_target_len( tmap_map_sam_t *s) +{ + s->target_len = 0; + int j; + for (j = 0; j < s->n_cigar; j++) + { + switch (TMAP_SW_CIGAR_OP (s->cigar [j])) + { + case BAM_CMATCH: + case BAM_CDEL: + s->target_len += TMAP_SW_CIGAR_LENGTH(s->cigar[j]); + break; + default: + break; + } + } +} static int tmap_map_get_amplicon(tmap_refseq_t *refseq, @@ -108,21 +130,30 @@ tmap_map_get_amplicon(tmap_refseq_t *refseq, } +// use softclip settings from tmap parameters +// disallow 3' softclip, if +// - adapter bases are present at 3' (sequencing reached the adapter) +// - and there are more of them than max_adapter_bases_for_soft_clipping, +// - and there is an insert (sequence between key abd barcode and before 3' adapter +// - and sequence is more than 5 bases longer than the insert static void -tmap_map_util_set_softclip(tmap_map_opt_t *opt, tmap_seq_t *seq, int32_t *softclip_start, int32_t *softclip_end) -{ - (*softclip_start) = (TMAP_MAP_OPT_SOFT_CLIP_LEFT == opt->softclip_type || TMAP_MAP_OPT_SOFT_CLIP_ALL == opt->softclip_type) ? 1 : 0; - (*softclip_end) = (TMAP_MAP_OPT_SOFT_CLIP_RIGHT == opt->softclip_type || TMAP_MAP_OPT_SOFT_CLIP_ALL == opt->softclip_type) ? 1 : 0; - // check if the ZB tag is present... - if(TMAP_SEQ_TYPE_SAM == seq->type || TMAP_SEQ_TYPE_BAM == seq->type) { // SAM/BAM - tmap_sam_t *sam = seq->data.sam; - int32_t zb = tmap_sam_get_zb(sam); - int32_t za = tmap_sam_get_za(sam); - // keep 3' soft clipping on if zb tag does not exists or there are too few adapter bases. - if(-1 != zb && opt->max_adapter_bases_for_soft_clipping < zb && -1 != za && sam->seq->l >= za-5) { - (*softclip_end) = 0; - } - } +tmap_map_util_set_softclip (tmap_map_opt_t *opt, tmap_seq_t *seq, int32_t *softclip_start, int32_t *softclip_end) +{ + (*softclip_start) = (TMAP_MAP_OPT_SOFT_CLIP_LEFT == opt->softclip_type || TMAP_MAP_OPT_SOFT_CLIP_ALL == opt->softclip_type) ? 1 : 0; + (*softclip_end) = (TMAP_MAP_OPT_SOFT_CLIP_RIGHT == opt->softclip_type || TMAP_MAP_OPT_SOFT_CLIP_ALL == opt->softclip_type) ? 1 : 0; + // check if the ZB tag is present... + if (TMAP_SEQ_TYPE_SAM == seq->type || TMAP_SEQ_TYPE_BAM == seq->type) // SAM/BAM + { + tmap_sam_t *sam = seq->data.sam; + int32_t zb = tmap_sam_get_zb (sam); + int32_t za = tmap_sam_get_za (sam); + // keep 3' soft clipping on if zb tag does not exists or there are too few adapter bases. + if (-1 != zb + && opt->max_adapter_bases_for_soft_clipping < zb + && -1 != za + && sam->seq->l >= za - 5) + (*softclip_end) = 0; + } } void @@ -133,6 +164,7 @@ tmap_map_sam_init(tmap_map_sam_t *s) // nullify s->algo_id = TMAP_MAP_ALGO_NONE; s->ascore = s->score = INT32_MIN; + //s->fivep_offset = 0; } @@ -196,6 +228,10 @@ tmap_map_sam_destroy(tmap_map_sam_t *s) free(s->cigar); s->cigar = NULL; s->n_cigar = 0; + free (s->orig_cigar); + s->orig_cigar = NULL; + s->n_orig_cigar = 0; + } tmap_map_sams_t * @@ -528,8 +564,9 @@ tmap_map_sam_print(tmap_seq_t *seq, tmap_refseq_t *refseq, tmap_map_sam_t *sam, mate_strand, mate_seqid, mate_pos, mate_tlen, sam->mapq, sam->cigar, sam->n_cigar, sam->score, sam->ascore, sam->pscore, nh, sam->algo_id, sam->algo_stage, - "\tXS:i:%d", - sam->score_subo); + "\tXS:i:%d\tXZ:i:%d", + sam->score_subo, + sam->fivep_offset); break; case TMAP_MAP_ALGO_MAPVSW: return tmap_sam_convert_mapped(seq, sam_flowspace_tags, bidirectional, seq_eq, refseq, @@ -1076,7 +1113,7 @@ tmap_map_util_mapq(tmap_map_sams_t *sams, int32_t seq_len, tmap_map_opt_t *opt, // ACGTNBDHKMRSVWYN // This gives a mask for match iupac characters versus A/C/G/T/N // A C G T N B D H K M R S V W Y N -static int32_t matrix_iupac_mask[89] = { +static int32_t matrix_iupac_mask [89] = { 1, 0, 0, 0, 1, 0, 1, 1, 0, 1, 1, 0, 1, 1, 0, 1, // A 0, 1, 0, 0, 1, 1, 0, 1, 0, 1, 0, 1, 1, 0, 1, 1, // C 0, 0, 1, 0, 1, 1, 1, 0, 1, 0, 1, 1, 1, 0, 0, 1, // G @@ -1096,6 +1133,13 @@ static int32_t matrix_iupac_mask[89] = { (par).band_width = (opt)->bw; \ } while(0) +int32_t matrix_iupac [80]; // this does not change, can be global +void tmap_map_util_populate_sw_par (tmap_sw_param_t* par, tmap_map_opt_t* opt) +{ + par->matrix = matrix_iupac; + __map_util_gen_ap_iupac (*par, opt); +} + static inline void tmap_map_util_sw_get_start_and_end_pos(tmap_map_sam_t *sam, int32_t seq_len, uint8_t strand, uint32_t *start_pos, uint32_t *end_pos) @@ -1111,19 +1155,22 @@ tmap_map_util_sw_get_start_and_end_pos(tmap_map_sam_t *sam, int32_t seq_len, uin // NB: this function unrolls banding in some cases static int32_t -tmap_map_util_sw_gen_score_helper(tmap_refseq_t *refseq, tmap_map_sams_t *sams, - tmap_seq_t *seq, tmap_map_sams_t *sams_tmp, - int32_t *idx, int32_t start, int32_t end, - uint8_t strand, tmap_vsw_t *vsw, - int32_t seq_len, uint32_t start_pos, uint32_t end_pos, - int32_t *target_mem, uint8_t **target, - int32_t softclip_start, int32_t softclip_end, - int32_t prev_n_best, - int32_t max_seed_band, // NB: this may be modified as banding is unrolled - int32_t prev_score, // NB: must be greater than or equal to the scoring threshold - tmap_vsw_opt_t *vsw_opt, - tmap_rand_t *rand, - tmap_map_opt_t *opt) +tmap_map_util_sw_gen_score_helper +( + tmap_refseq_t *refseq, tmap_map_sams_t *sams, + tmap_seq_t *seq, tmap_map_sams_t *sams_tmp, + int32_t *idx, int32_t start, int32_t end, + uint8_t strand, tmap_vsw_t *vsw, + int32_t seq_len, uint32_t start_pos, uint32_t end_pos, + int32_t *target_mem, uint8_t **target, + int32_t softclip_start, int32_t softclip_end, + int32_t prev_n_best, + int32_t max_seed_band, // NB: this may be modified as banding is unrolled + int32_t prev_score, // NB: must be greater than or equal to the scoring threshold + tmap_vsw_opt_t *vsw_opt, + tmap_rand_t *rand, + tmap_map_opt_t *opt +) { tmap_map_sam_t tmp_sam; uint8_t *query; @@ -1223,31 +1270,101 @@ tmap_map_util_sw_gen_score_helper(tmap_refseq_t *refseq, tmap_map_sams_t *sams, */ // NB: this aligns in the sequencing direction - tmp_sam.score = tmap_vsw_process_fwd(vsw, query, qlen, (*target), tlen, - &tmp_sam.result, &overflow, opt->score_thr, 1); - - if(1 < tmp_sam.result.n_best) { - // What happens if soft-clipping or not soft-clipping causes two - // alignments to be equally likely? So while we could set the scores to be - // similar, we can also down-weight the score a little bit. - tmp_sam.score_subo = tmp_sam.score - opt->pen_gapo - opt->pen_gape; - //tmp_sam.score_subo = tmp_sam.score - 1; + // DVK: bug #11386 + { + const int16_t TARGET_EDGE_ALLOWANCE = 4; + const int16_t MAX_TARGET_LEN_FOR_VSW = 32000; + int8_t target_causes_read_clipping; + int8_t first_run = 1; + int32_t score = 0, prev_score = 0; + uint32_t new_end_pos, new_start_pos; + tmap_vsw_result_t result; + do + { + // initial assumption + target_causes_read_clipping = 0; + + // initialize the bounds - why? + result.query_start = result.query_end = 0; + result.target_start = result.target_end = 0; + score = tmap_vsw_process_fwd(vsw, query, qlen, (*target), tlen, + &result, &overflow, opt->score_thr, 1); + + if (1 == overflow) + return INT32_MIN; + + if (!first_run && score <= prev_score) // check for zone edge crossing on first run always, then only if score is improved + break; // if score did not improve, get out and keep last result/score in tmp_sam + + // query clip may be recoverable if it has 3' clip and alignment goes to target zone end + new_end_pos = end_pos; + new_start_pos = start_pos; + if (tlen - result.target_end < TARGET_EDGE_ALLOWANCE && qlen > result.query_end) + { + // extend target if possible + new_end_pos += qlen - result.query_end + TARGET_EDGE_ALLOWANCE; + if(refseq->annos[sams->sams[end].seqid].len < new_end_pos) + { + new_end_pos = refseq->annos[sams->sams[end].seqid].len; // one-based + } + } + // similarly process 5' clips + if (result.target_start < TARGET_EDGE_ALLOWANCE && result.query_start > 0) + { + if (result.query_start + TARGET_EDGE_ALLOWANCE > new_start_pos) + new_start_pos -= result.query_start + TARGET_EDGE_ALLOWANCE; + else + new_start_pos = 1; + } + if (new_end_pos > end_pos || new_start_pos < start_pos ) + { + end_pos = new_end_pos; + start_pos = new_start_pos; + // get the target sequence + tlen = end_pos - start_pos + 1; + if((*target_mem) < tlen) + { // more memory? + (*target_mem) = tlen; + tmap_roundup32((*target_mem)); + (*target) = tmap_realloc((*target), sizeof(uint8_t)*(*target_mem), "target"); + } + // NB: IUPAC codes are turned into mismatches + if(NULL == tmap_refseq_subseq2(refseq, sams->sams[end].seqid+1, start_pos, end_pos, (*target), 1, NULL)) + tmap_error("bug encountered", Exit, OutOfRange); + + // reverse compliment the target + if (1 == strand) + tmap_reverse_compliment_int((*target), tlen); + + target_causes_read_clipping = 1; + } + tmp_sam.score = score; + tmp_sam.result = result; + prev_score = score; + first_run = 0; + if (start_pos + MAX_TARGET_LEN_FOR_VSW < end_pos) + break; } - if(1 == is_long_hit) { - // if we have many seeds that span a large tandem repeat, then we should - // downweight the sub-optimal score - tmp_sam.score_subo = tmp_sam.score - opt->pen_gapo - opt->pen_gape; + while (target_causes_read_clipping); + } - /* - fprintf(stderr, "start_pos=%d end_pos=%d start=%d end=%d score=%d n_best=%d\n", - start_pos, end_pos, start, end, tmp_sam.score, tmp_sam.result.n_best); - */ - if(1 == overflow) { - return INT32_MIN; - //tmap_error("bug encountered", Exit, OutOfRange); + if (1 < tmp_sam.result.n_best) + { + // What happens if soft-clipping or not soft-clipping causes two + // alignments to be equally likely? So while we could set the scores to be + // similar, we can also down-weight the score a little bit. + tmp_sam.score_subo = tmp_sam.score - opt->pen_gapo - opt->pen_gape; + //tmp_sam.score_subo = tmp_sam.score - 1; + } + if (1 == is_long_hit) + { + // if we have many seeds that span a large tandem repeat, then we should + // downweight the sub-optimal score + tmp_sam.score_subo = tmp_sam.score - opt->pen_gapo - opt->pen_gape; } + if(opt->score_thr <= tmp_sam.score) { // NB: we could also specify 'prev_score <= tmp_sam.score' int32_t add_current = 1; // yes, by default @@ -1257,7 +1374,7 @@ tmap_map_util_sw_gen_score_helper(tmap_refseq_t *refseq, tmap_map_sams_t *sams, int32_t t_start = start; int32_t t_end_pos = end_pos; uint32_t t_start_pos = start_pos; - + if(1 == opt->unroll_banding // unrolling is turned on && 0 <= max_seed_band // do we have enough bases to unrooll && 1 < end - start + 1 // are there enough seeds in this group @@ -1335,7 +1452,7 @@ tmap_map_util_sw_gen_score_helper(tmap_refseq_t *refseq, tmap_map_sams_t *sams, if(1 == add_current) { tmap_map_sam_t *s = NULL; - + if(sams_tmp->n <= (*idx)) { tmap_map_sams_realloc(sams_tmp, (*idx)+1); //tmap_error("bug encountered", Exit, OutOfRange); @@ -1402,7 +1519,8 @@ tmap_map_util_sw_gen_score_helper(tmap_refseq_t *refseq, tmap_map_sams_t *sams, return tmp_sam.score; } -typedef struct { +typedef struct +{ uint32_t seqid; int8_t strand; int32_t start; @@ -1414,13 +1532,16 @@ typedef struct { } tmap_map_util_gen_score_t; tmap_map_sams_t * -tmap_map_util_sw_gen_score(tmap_refseq_t *refseq, - tmap_seq_t *seq, - tmap_map_sams_t *sams, - tmap_seq_t **seqs, - tmap_rand_t *rand, - tmap_map_opt_t *opt, - int32_t *num_after_grouping) +tmap_map_util_sw_gen_score +( + tmap_refseq_t *refseq, + tmap_seq_t *seq, + tmap_map_sams_t *sams, + tmap_seq_t **seqs, + tmap_rand_t *rand, + tmap_map_opt_t *opt, + int32_t *num_after_grouping +) { int32_t i, j; int32_t start, end; @@ -1814,11 +1935,17 @@ tmap_map_util_sw_gen_score(tmap_refseq_t *refseq, return sams_tmp; } + static void -tmap_map_util_keytrim(uint8_t *query, int32_t qlen, - uint8_t *target, int32_t tlen, - int8_t strand, uint8_t key_base, - tmap_map_sam_t *s) +tmap_map_util_keytrim +( + uint8_t *query, int32_t qlen, + uint8_t *target, int32_t tlen, + int8_t strand, + uint8_t key_base, + tmap_map_sam_t *s, + tmap_map_stats_t* stat +) { int32_t j, k, l; int32_t op, op_len; @@ -1943,1951 +2070,3360 @@ tmap_map_util_keytrim(uint8_t *query, int32_t qlen, } } +// DK note: implementation is very inefficient, could be done in linear time static void -tmap_map_util_merge_adjacent_cigar_operations(tmap_map_sam_t *s) +tmap_map_util_merge_adjacent_cigar_operations (tmap_map_sam_t *s) { - int32_t i, j; - // merge adjacent cigar operations that have the same value - for(i=s->n_cigar-2;0<=i;i--) { - if(TMAP_SW_CIGAR_OP(s->cigar[i]) == TMAP_SW_CIGAR_OP(s->cigar[i+1])) { - TMAP_SW_CIGAR_STORE(s->cigar[i], TMAP_SW_CIGAR_OP(s->cigar[i]), TMAP_SW_CIGAR_LENGTH(s->cigar[i]) + TMAP_SW_CIGAR_LENGTH(s->cigar[i+1])); - // shift down - for(j=i+1;jn_cigar-1;j++) { - s->cigar[j] = s->cigar[j+1]; - } - s->n_cigar--; - } - } + int32_t i, j; + // merge adjacent cigar operations that have the same value + for (i = s->n_cigar-2; 0 <= i; i--) + { + if (TMAP_SW_CIGAR_OP (s->cigar [i]) == TMAP_SW_CIGAR_OP (s->cigar [i+1])) + { + TMAP_SW_CIGAR_STORE (s->cigar[i], TMAP_SW_CIGAR_OP(s->cigar[i]), TMAP_SW_CIGAR_LENGTH(s->cigar[i]) + TMAP_SW_CIGAR_LENGTH(s->cigar[i+1])); + // shift down + for (j = i + 1; j < s->n_cigar - 1; j++) + s->cigar [j] = s->cigar [j + 1]; + s->n_cigar--; + } + } } -//ZZ: finding #S#M#I or #S#M#D alignment for the two sequences allowing at most M mismatch. +// ZZ: finding #S#M#I or #S#M#D alignment for the two sequences allowing at most M mismatch. +// DK: returns 1 if found, 0 if not +// DK: above cigars are for reverse orientation (at the 3' of reverse - oriented read), appearing at the beginning of the whole alignment +// DK: for forward - oriented reads, that would be #I#M#S and #D#M#S, appearing on the end of the whole alignment + static int -tmap_map_util_one_gap(uint8_t *query, int32_t qlen, uint8_t *target, int32_t tlen, int32_t min_size, int max_q, int max_t, int max_MM, int max_indel, - int8_t strand, int *n_mismatch, int *indel_size, int *is_ins, int *softclip) +tmap_map_util_one_gap ( + uint8_t *query, // query sequence (numeric-encoded as [ACGT], 1 byte per base) + int32_t qlen, // query length + uint8_t *target, // target sequence (numeric-encoded as [ACGT], 1 byte per base) + int32_t tlen, // target length + int32_t min_size, // min anchor size (min length of M segment) + int max_q, // max softclip in query + int max_t, // max clip (skipped bases, or shift) in target. This + int max_MM, // maximum allowed mismatches in the searched structure + int max_indel, // maximal number of indels (length of I or D) + int8_t strand, // strand (== orientation): 0 - forward, 1 - reverse + int *n_mismatch, // found number of mismatches + int *indel_size, // number of bases in Indel segment + int *is_ins, // 1 == Ins, 0 == Del + int *softclip, // length of prefix softclip + int pad, // the extension on either size of target buffer that is safe to access. This function checks this many bases as continuation of M segment as if no indel was present + int *extra_match) // number of consecutive matches after M segment end (check relation with pad. Are these bases in the pad??) { int i, j, q, qq, t, tt, inc; int qini, tini; - if (strand == 0) { - qini = qlen-1; tini = tlen-1; - inc = -1; - } else { - qini = tini = 0; inc = 1; - } - if (qlen < min_size || tlen < min_size) return 0; - if (qlen-min_size < max_q) max_q= qlen-min_size; - if (tlen-min_size < max_t) max_t = tlen-min_size; - if (qlen*3 < max_indel) max_indel = qlen*3; - if (/*max_MM > 1 &&*/ qlen-max_q+1 < 8*max_MM) max_MM = (qlen-max_q+1)/8; // 7-14 bp allow 1 MM, 15 allow 2 etc. - if (qlen-tlen> max_indel) return 0; - if (tlen-max_t-qlen > max_indel) return 0; + if (strand == 0) + { + qini = qlen-1; + tini = tlen-1; + inc = -1; + } + else + { + qini = tini = 0; + inc = 1; + } + if (qlen < min_size || tlen < min_size) + return 0; + if (qlen - min_size < max_q) + max_q = qlen - min_size; + if (tlen - min_size < max_t) + max_t = tlen - min_size; + //if (qlen*3 < max_indel) max_indel = qlen*3; + if (/*max_MM > 1 &&*/ qlen - max_q + 1 < 8 * max_MM) + max_MM = (qlen - max_q + 1) / 8; // 7-14 bp allow 1 MM, 15 allow 2 etc. + if (qlen - tlen > max_indel) + return 0; + if (tlen - max_t - qlen > max_indel) + return 0; //debug /* char code[] = "ACGT"; fprintf(stderr, "tlen=%d qlen=%d\n", tlen, qlen); - for (i = 0; i < tlen; i++) { - fprintf(stderr, "%c", code[target[i]]); - } + for (i = 0; i < tlen; i++) + fprintf(stderr, "%c", code[target[i]]); fprintf(stderr, "\n"); - for (i = 0; i < qlen; i++) { - fprintf(stderr,"%c", code[query[i]]); - } + for (i = 0; i < qlen; i++) + fprintf(stderr,"%c", code[query[i]]); fprintf(stderr, "\n"); */ - for (i = 0, t = tini; i < max_t; i++, t+= inc) { - int last_M = -1; - int nM = 0; - for (j = i, q = 0, qq = qini, tt = t; j < tlen && q < qlen; j++, q++, qq += inc, tt+= inc) { - if (query[qq] == target[tt]) continue; - if (q < max_q) last_M = q; - else { - nM++; - if (nM > max_MM) break; - } - } - if (j == tlen || q == qlen) { - // find one - *softclip = last_M+1; - *n_mismatch = nM; - if (j == tlen) { - *indel_size = qlen-q; - *is_ins = 1; - } else { - *indel_size = tlen-j; - *is_ins = 0; - } - if (*indel_size > max_indel) return 0; - if (*indel_size == 0) return 0; - return 1; - } + for (i = 0, t = tini; i < max_t; i++, t+= inc) + { + int last_M = -1; // last_mismatch + int nM = 0; // number_of_mismatches + for (j = i, q = 0, qq = qini, tt = t; j < tlen && q < qlen; j++, q++, qq += inc, tt+= inc) + { + if (query [qq] == target [tt]) + continue; + if (q < max_q) + last_M = q; + else + { + nM++; + if (nM > max_MM) + break; + } + } + if (j == tlen || q == qlen) + { + // find one + *softclip = last_M + 1; + *n_mismatch = nM; + *extra_match = 0; + if (j == tlen) + { + *indel_size = qlen-q; + *is_ins = 1; + } + else + { + *indel_size = tlen - j; + *is_ins = 0; + } + if (*indel_size > max_indel) + return 0; + if (*indel_size == 0) + return 0; + // here for strand == 0 we may go into negative index, intentionally. careful! + // extend beyond the parallel + // DK: for this to work correctly, passed pad should always be confined within the passed query and target buffers, with corrections for clips and indels. Is this the case - need to check calling code.!? + //fprintf(stderr, "find gap strand %d pad %d %d %d %d\n", strand, pad, j, tlen, qlen); + for (j = 0; j < pad; j++, qq += inc, tt += inc) + { + //fprintf(stderr, "%d %d %d %d\n", qq, tt, query[qq], target[tt]); + if (query [qq] != target [tt]) + break; + (*extra_match) += 1; + } + if (*indel_size > 3 * (qlen + (*extra_match))) // reject indels 3 or more times longer than query + matching 'pad' + return 0; + return 1; + } } return 0; } +// swaps two integers (passed by pointers) +static void exchange(int32_t *s, int32_t *t) +{ + int32_t temp = *s; *s = *t; *t = temp; +} -static void -tmap_map_util_end_repair(tmap_seq_t *seq, uint8_t *query, int32_t qlen, - uint8_t *target_prev, int32_t tlen, - int8_t strand, - tmap_sw_path_t *path, - tmap_refseq_t *refseq, - tmap_map_sam_t *s, - tmap_map_opt_t *opt, int repair5p) -{ - int32_t i, cigar_i; - int32_t op, op_len, cur_len; - int32_t cur_op, cur_op_len, cur_cigar_i, cur_cur_len, target_adj; - int32_t softclip_start, softclip_end; - int32_t old_score, new_score; - int32_t start_pos, end_pos; - // scoring matrix - int32_t matrix[25], matrix_iupac[80]; - tmap_sw_param_t par, par_iupac; - int32_t path_len = 0; - uint32_t *cigar = NULL; - int32_t n_cigar = 0; - int32_t found = 0; - - // TODO: use outside memory - uint8_t *target = NULL; - int32_t target_mem = 0; - int32_t conv = 0; - - // PARAMETERS - int32_t max_match_len = 5; // the maximum # of query bases to consider for the sub-alignment - int32_t num_prev_bases = 2; // the maximum number of bases prior to the alignment to include in the target - - // check if we allow soft-clipping on the 5' end - tmap_map_util_set_softclip(opt, seq, &softclip_start, &softclip_end); - if (opt->end_repair > 2) { - if (repair5p == 0 && 1 == softclip_end) return; // allow softclip, then no need to repair. - if (repair5p == 1 && 1 == softclip_start) return; - if (repair5p) strand = (!strand); - old_score = 0; - found = 0; - int worst = 0; - int ind = 0, nMM = 0, /*nc = 0,*/ nqb = 0, ntb = 0, nCC, target_red = 0; - int tb, qb, inc; - int i_c; - int i, nn_cigar=0, total_scl = 0; - int max_gap = 0, num_gap = 0; // ZZ, for TS-10540 - if (0 != strand) { tb = qb = 0; inc = 1;} - else {tb = tlen-1; qb = qlen-1; inc = -1;} - for (i_c = 0; i_c < s->n_cigar; i_c++) { - cigar_i = (0 != strand) ? i_c : s->n_cigar-1-i_c; - op = TMAP_SW_CIGAR_OP(s->cigar[cigar_i]); - op_len = TMAP_SW_CIGAR_LENGTH(s->cigar[cigar_i]); - if (op == BAM_CMATCH) { - for(i=0;iscore_match; - else old_score -= opt->pen_mm, found++; - if (old_score < worst) { worst = old_score; ind = i+1; nMM = found; target_red =ntb+1; nn_cigar = i_c; total_scl = nqb+1;} - } - } else { - if (op == BAM_CDEL) { - ntb += op_len; - tb += inc*op_len; - } else if (op == BAM_CINS) { - nqb += op_len; - qb += inc*op_len; - } else break; - old_score -= opt->pen_gapo+opt->pen_gape*op_len; - found += op_len; - if (old_score < worst) { worst = old_score; ind = 0; nMM = found; target_red =ntb; nn_cigar = i_c; total_scl = nqb; - num_gap++; if (op_len > max_gap) max_gap = op_len; // ZZ, for TS-10540 - } - } - if (old_score > 6*opt->score_match) break; // no need go further. - } - if (worst >= 0) return; - nCC = target_red+total_scl+1; - if (num_gap < 3 && max_gap >= 3) { - int del = max_gap/15; //increase the error by 1 for each 15 bp of the gap. - int adj = max_gap-1-del; - /* int adj = max_gap-1;*/ - nMM -= adj; nCC-= adj; - } - nCC /= 2; - int maxMM = (nCC-2)*opt->end_repair/100+2; - //if (repair5p) maxMM = (nCC-2)*50/100+2; // temp, try symmetric - if (maxMM < 2) maxMM = 2; // no need? always at least 2 - if (repair5p && total_scl <= 5) maxMM = nMM+1; // for 5', do not repair short stretch - if (nCC < maxMM) maxMM = nCC; - if (nMM >= maxMM) { - s->score -= worst; - cigar_i = (0 != strand) ? nn_cigar : s->n_cigar-1-nn_cigar; - op_len = TMAP_SW_CIGAR_LENGTH(s->cigar[cigar_i]); - if (ind == op_len || ind == 0) { - if (nn_cigar == s->n_cigar-1) return; // all are removed, something not quite right. Give up - if (nn_cigar == s->n_cigar-2) { - if (TMAP_SW_CIGAR_OP(s->cigar[cigar_i+inc]) == BAM_CSOFT_CLIP) return; // something wrong, will become XSYS?? - } - TMAP_SW_CIGAR_STORE(s->cigar[cigar_i], BAM_CSOFT_CLIP, total_scl); - s->n_cigar -= nn_cigar; - } else { - TMAP_SW_CIGAR_STORE(s->cigar[cigar_i], BAM_CMATCH, op_len-ind); - if (nn_cigar == 0) { - s->cigar = tmap_realloc(s->cigar, sizeof(uint32_t)*(1 + s->n_cigar), "s->cigar"); - if (0 != strand) { - for (i = s->n_cigar-1; i >= 0; i--) s->cigar[i+1] = s->cigar[i]; - TMAP_SW_CIGAR_STORE(s->cigar[0], BAM_CSOFT_CLIP, total_scl); - } else { - TMAP_SW_CIGAR_STORE(s->cigar[s->n_cigar], BAM_CSOFT_CLIP, total_scl); - } - s->n_cigar++; - } else { - cigar_i -= inc; // move back one - TMAP_SW_CIGAR_STORE(s->cigar[cigar_i], BAM_CSOFT_CLIP, total_scl); - s->n_cigar -= nn_cigar-1; - } - } - if (0 != strand && cigar_i > 0) { - for (i = 0; i < s->n_cigar; i++) { - s->cigar[i] = s->cigar[i+cigar_i]; - } - } - if (0 != strand) s->pos += target_red; - s->target_len -= target_red; - // do one large gap label:oneGapZZ - uint32_t ampl_start, ampl_end; - if (refseq->bed_exist && total_scl > 5 && - tmap_map_get_amplicon(refseq, s->seqid, s->pos, s->pos+s->target_len-1, &l_start, &l_end, strand)) { - //fprintf(stderr, "%d %d \n", ampl_start, ampl_end); - int need_do = 1; - int half_buffer = /*6*/ opt->amplicon_overrun; // outside freedom. - int buffer = half_buffer+3; - uint32_t start, end; - if (strand == 0) { - start = s->pos + s->target_len + 1; - end = ampl_end+half_buffer; - if (end > refseq->annos[s->seqid].len) end = refseq->annos[s->seqid].len; - query += qlen-total_scl; - } else { - if (ampl_start <= half_buffer) start = 1; - else start = ampl_start-half_buffer; - end = s->pos; - } - tlen = end-start+1; - //fprintf(stderr, "%d %d \n", start, end); - if (tlen > 5) { - if(target_mem < tlen) { // more memory? - target_mem = tlen; - tmap_roundup32(target_mem); - target = tmap_realloc(target, sizeof(uint8_t)*target_mem, "target"); - } - if(NULL == tmap_refseq_subseq2(refseq, s->seqid+1, start, end, target, 1, NULL)) { - tmap_bug(); - } - } else need_do = 0; - int softclip, target_adj, n_mismatch, indel_size, is_ins; - if (need_do && tmap_map_util_one_gap(query, total_scl, target, tlen, opt->min_anchor_large_indel_rescue /*anchor size*/, 2/*int max_q*/, buffer /*int max_t*/, 3 /*maxNM*/, opt->max_one_large_indel_rescue, /*int max_indel*/ - strand, &n_mismatch, &indel_size, &is_ins, &softclip)) { - //adjust cigar - int match_size; - int target_adj; // how many additional target bases aligned. - if (is_ins) match_size = total_scl-softclip-indel_size; - else match_size = total_scl-softclip; - target_adj = match_size; - if (!is_ins) target_adj += indel_size; - int s_adj = (match_size-n_mismatch)*opt->score_match-n_mismatch*opt->pen_mm-opt->pen_gapo-opt->pen_gape; - s->score += s_adj; - int inc_cigar = 1; - if (softclip> 0) inc_cigar++; - s->cigar = tmap_realloc(s->cigar, sizeof(uint32_t)*(inc_cigar + s->n_cigar), "s->cigar"); - if (strand == 0) { - TMAP_SW_CIGAR_STORE(s->cigar[s->n_cigar-1], is_ins? BAM_CINS: BAM_CDEL , indel_size); - TMAP_SW_CIGAR_STORE(s->cigar[s->n_cigar], BAM_CMATCH, match_size); - s->n_cigar++; - if (softclip> 0) { - TMAP_SW_CIGAR_STORE(s->cigar[s->n_cigar], BAM_CSOFT_CLIP, softclip); - s->n_cigar++; - } - } else { - for (i = s->n_cigar-1; i >= 0; i--) s->cigar[i+inc_cigar] = s->cigar[i]; - i = 0; - if (softclip> 0) TMAP_SW_CIGAR_STORE(s->cigar[i++], BAM_CSOFT_CLIP, softclip); - TMAP_SW_CIGAR_STORE(s->cigar[i++], BAM_CMATCH, match_size); - TMAP_SW_CIGAR_STORE(s->cigar[i], is_ins? BAM_CINS: BAM_CDEL , indel_size); - s->pos -= target_adj; - s->n_cigar+= inc_cigar; - } - s->target_len += target_adj; - free(target); - } - } - } +static int +trim_read_bases ( + tmap_map_sam_t *s, + int extra_match, + int from_head, + int *is_ins, + int *indel_len) +{ + if (extra_match <= 0) + return 0; + int32_t indel_type, other_type; - /* - cigar_i = (0 != strand) ? 0 : (s->n_cigar - 1); - op = TMAP_SW_CIGAR_OP(s->cigar[cigar_i]); - op_len = TMAP_SW_CIGAR_LENGTH(s->cigar[cigar_i]); - if(op != BAM_CMATCH) return; // right now just check terminal matches, will extend to other. - old_score = 0; - found = 0; // did we find mismatches? - int worst = 0; - cur_len = op_len; - int ind = 0, nMM = 0; - if(0 != strand) { // reverse - for(i=0;iscore_match; - else old_score -= opt->pen_mm, found++; - if (old_score < worst) { worst = old_score; ind = i+1; nMM = found;} - } + indel_type = BAM_CDEL; other_type = BAM_CINS; + if (!(*is_ins)) + exchange(&indel_type, &other_type); + + int inc, init, i, j; + int dec = 0; + int in_len = *indel_len; + + if (from_head) + { + init = 1; + inc = 1; } - else { // forward - for(i=0;iscore_match; - else old_score -= opt->pen_mm, found++; - if (old_score < worst) { worst = old_score; ind = i+1; nMM = found;} - } + else + { + init = s->n_cigar-2; + inc = -1; } - if (worst >= 0) return; // local alignment is ok - int maxMM = 2; - if (ind > 5) maxMM = (ind-2)*opt->end_repair/100+2; - if (ind < maxMM) maxMM = ind; - if (nMM >= maxMM) { // trim - s->score -= worst; - if (ind == op_len) { // remove the whole cigar - if (s->n_cigar == 1) return; - TMAP_SW_CIGAR_STORE(s->cigar[cigar_i], BAM_CSOFT_CLIP, op_len); - } else { - TMAP_SW_CIGAR_STORE(s->cigar[cigar_i], BAM_CMATCH, op_len-ind); - s->cigar = tmap_realloc(s->cigar, sizeof(uint32_t)*(1 + s->n_cigar), "s->cigar"); - if (0 != strand) { - int i; - for (i = s->n_cigar-1; i >= 0; i--) s->cigar[i+1] = s->cigar[i]; - TMAP_SW_CIGAR_STORE(s->cigar[0], BAM_CSOFT_CLIP, ind); - } else { - TMAP_SW_CIGAR_STORE(s->cigar[s->n_cigar], BAM_CSOFT_CLIP, ind); - } - s->n_cigar++; - } - if (0 != strand) s->pos += ind; - s->target_len -= ind; - //fprintf(stderr, "ZZ tlen%d %d %d %d worst %d MM=%d %d\n", s->target_len, ind, s->score, s->pos, worst, maxMM, nMM); + int32_t op, op_len; + for (i = 0, j = init; extra_match > 0 && i < s->n_cigar-1; i++, j += inc) + { + op = TMAP_SW_CIGAR_OP (s->cigar[j]); + op_len = TMAP_SW_CIGAR_LENGTH (s->cigar[j]); + //fprintf(stderr, "%d %d extra_match=%d j=%d indel=%d other=%d\n", op, op_len, extra_match, j, indel_type, other_type); + if (op == other_type) + { + in_len += op_len; + dec++; + } + else + { + //if (op == BAM_CMATCH || op == indel_type || op == BAM_CSOFT_CLIP) are the only choice + if (op == indel_type) + { + int x = extra_match > op_len ? op_len : extra_match; + if (x > in_len) + { + extra_match -= in_len; + in_len = op_len - in_len; + exchange (&indel_type, &other_type); // switch indel type now, tricky! ZZ + dec++; // put the indel as the ending one. so remove this op. + continue; + } + in_len -= x; + } + if (extra_match >= op_len) + { + extra_match -= op_len; + dec++; + } + else + { + op_len -= extra_match; + TMAP_SW_CIGAR_STORE(s->cigar[j], op, op_len); + break; + } + } } + if (i >= s->n_cigar-1) + tmap_error("extra match exceed cigar length", Exit, OutOfRange); + /* may not need the following + op = TMAP_SW_CIGAR_OP(s->cigar[j]); + op_len = TMAP_SW_CIGAR_LENGTH(s->cigar[j]); + + if (op == other_type) {dec++; in_len += op_len;} */ - return; - } - // do not perform if so - if (repair5p) return; - if(1 == softclip_start) return; + *is_ins = (indel_type == BAM_CINS)? 0:1; + *indel_len = in_len; + return dec; +} - // check the first cigar op - cigar_i = (0 == strand) ? 0 : (s->n_cigar - 1); - op = TMAP_SW_CIGAR_OP(s->cigar[cigar_i]); - op_len = TMAP_SW_CIGAR_LENGTH(s->cigar[cigar_i]); - if(op != BAM_CMATCH) return; // ignore, since we care only about turning mismatches into indels +static uint32_t +tmap_map_util_end_repair ( + tmap_seq_t *seq, uint8_t *query, int32_t qlen, + uint8_t *target_prev, int32_t tlen, + int8_t strand, + tmap_sw_path_t **path_buf, + int32_t* path_buf_sz, + tmap_refseq_t *refseq, + tmap_map_sam_t *s, + tmap_map_opt_t *opt, + int repair5p, + tmap_map_stats_t* stat +) +{ + int32_t i, cigar_i; + int32_t op, op_len, cur_len; + int32_t cur_op, cur_op_len, cur_cigar_i, cur_cur_len, target_adj; + int32_t softclip_start, softclip_end; + int32_t old_score, new_score; + int32_t start_pos, end_pos; + // scoring matrix + int32_t matrix [25], matrix_iupac [80]; + tmap_sw_param_t par, par_iupac; + int32_t path_len = 0; + uint32_t *cigar = NULL; + int32_t n_cigar = 0; + int32_t found = 0; + + // TODO: use outside memory + uint8_t *target = NULL; + int32_t target_mem = 0; + int32_t conv = 0; + + // PARAMETERS + int32_t max_match_len = 5; // the maximum # of query bases to consider for the sub-alignment + int32_t num_prev_bases = 2; // the maximum number of bases prior to the alignment to include in the target + + // side and orientation for stats + int32_t sori = repair5p?(s->strand?R5P:F5P):(s->strand?R3P:F3P); + int32_t repaired = 0; // return value: 0 for no change, 1 for softclip only, 2 for indel salvage. + + // check if we allow soft-clipping on the 5' end + tmap_map_util_set_softclip (opt, seq, &softclip_start, &softclip_end); + int8_t o_strand = strand; + if (opt->end_repair > 2) // Modern End-Repair, invoked when --end-repair parameter is above 2 (and denotes percent of mismatches above which to trim the alignment) + { + if (repair5p == 0 && 1 == softclip_end) + return repaired; // allow softclip, then no need to repair. + if (repair5p == 1 && 1 == softclip_start) + return repaired; + //fprintf(stderr, "repair5p=%d %d\n", repair5p, strand); + if (repair5p) + strand = (!strand); + old_score = 0; // score of original (passed in) alignment + found = 0; // number of non-matches (mismatches, inserts, deletes) found in alignment + int worst = 0; // worst alignment score encountered along the alignment + int ind = 0; // index of base within the cigar operation where worst alignment score is achieved + int nMM = 0; // number of mismatches in the part of alignment before worst score position + int nqb = 0; // number of query bases seen + int ntb = 0; // number of target bases seen + int nCC; // (?) NuCleotide Count - 'average' of target and query lengths involved in (old, passed in) alignment + int target_red = 0; // Target Reduction: the offset on target to the position of worst score + int tb, qb, inc; // target position, query position, increment (+1 | -1) for walking along target / query + int i_c; // index of the cigar operation + int i; // index of base within cigar operation + int nn_cigar = 0; // cigar operation index where worst alignment score is achieved + int total_scl = 0; // total soft clip (?) - actually the offset on query to the position of worst score + int max_gap = 0, num_gap = 0; // ZZ, for TS-10540 + + // remove softclip, shall not be necessary, remove later?? ZZ + + if (strand == 0) + { + op = TMAP_SW_CIGAR_OP (s->cigar [s->n_cigar - 1]); + op_len = TMAP_SW_CIGAR_LENGTH (s->cigar [s->n_cigar - 1]); + if (op == BAM_CSOFT_CLIP) + { + op = TMAP_SW_CIGAR_OP (s->cigar [s->n_cigar - 2]); + int32_t op_len1 = TMAP_SW_CIGAR_LENGTH (s->cigar [s->n_cigar - 2]); + if (op == BAM_CMATCH) + TMAP_SW_CIGAR_STORE (s->cigar [s->n_cigar - 2], op, op_len + op_len1); + else + return repaired; + s->n_cigar--; + tmap_map_util_set_target_len (s); + qlen += op_len; + tlen = s->target_len; + repaired = 1; + } + } + else + { + op = TMAP_SW_CIGAR_OP (s->cigar [0]); + op_len = TMAP_SW_CIGAR_LENGTH (s->cigar [0]); + if (op == BAM_CSOFT_CLIP) + { + op = TMAP_SW_CIGAR_OP (s->cigar [1]); + int32_t op_len1 = TMAP_SW_CIGAR_LENGTH (s->cigar [1]); + if (op == BAM_CMATCH) + { + TMAP_SW_CIGAR_STORE (s->cigar[1], op, op_len+op_len1); + s->pos -= op_len; + } + else + return repaired; + for (i = 0; i < s->n_cigar-1; i++) + s->cigar [i] = s->cigar [i+1]; + s->n_cigar--; + tmap_map_util_set_target_len (s); + query -= op_len; + target_prev -= op_len; + qlen += op_len; + tlen = s->target_len; + repaired = 1; + } + } - // get the amount of bases to adjust - cur_len = (max_match_len < op_len) ? max_match_len : op_len; - // NB: must include full HPs - while(cur_len < qlen && query[cur_len-1] == query[cur_len]) { // TODO: what about reference HPs? - cur_len++; - } - if(cur_len < qlen) { - cur_len++; // NB: include one more base to get left-justification correct - } + // evaluate if repairing makes sense + if (0 != strand) + { + tb = qb = 0; + inc = 1; + } + else + { + tb = tlen-1; + qb = qlen-1; + inc = -1; + } + for (i_c = 0; i_c < s->n_cigar; i_c++) + { + cigar_i = (0 != strand) ? i_c : s->n_cigar-1-i_c; + op = TMAP_SW_CIGAR_OP(s->cigar[cigar_i]); + op_len = TMAP_SW_CIGAR_LENGTH(s->cigar[cigar_i]); + if (op == BAM_CMATCH) + { + for (i = 0; i < op_len; i++, qb += inc, tb += inc, nqb++, ntb++) + { + if (qb == -1 || tb == -1) + tmap_bug (); + if (query [qb] == target_prev [tb]) + old_score += opt->score_match; + else + { + old_score -= opt->pen_mm; + found++; + } + if (old_score < worst) + { + worst = old_score; + ind = i + 1; + nMM = found; + target_red = ntb + 1; + nn_cigar = i_c; + total_scl = nqb + 1; + } + } + } + else + { + if (op == BAM_CDEL) + { + ntb += op_len; + tb += inc * op_len; + } + else if (op == BAM_CINS) + { + nqb += op_len; + qb += inc*op_len; + } + else + break; + old_score -= opt->pen_gapo + opt->pen_gape * op_len; + found += op_len; + if (old_score < worst) + { + worst = old_score; + ind = 0; + nMM = found; + target_red = ntb; + nn_cigar = i_c; + total_scl = nqb; + num_gap++; + if (op_len > max_gap) + max_gap = op_len; // ZZ, for TS-10540 + } + } + if (old_score > 6 * opt->score_match) + break; // no need go further. + } + if (worst >= 0) + return repaired; + nCC = target_red + total_scl + 1; + if (num_gap < 3 && max_gap >= 3) + { + int del = max_gap / 15; // increase the error by 1 for each 15 bp of the gap. + int adj = max_gap - 1 - del; + /* int adj = max_gap-1;*/ + nMM -= adj; + nCC -= adj; + } + nCC /= 2; + int maxMM = (nCC - 2) * opt->end_repair / 100 + 2; // DK: integer arithmetics ! + if (maxMM < 2) + maxMM = 2; // no need? always at least 2 + if (nCC < maxMM) + maxMM = nCC; + if (nMM >= maxMM) // observed (and adjusted) number of mismatches BEFORE THE WORST SCORE POSITION is over maximal tolerable number of mismatches + { + s->score -= worst; + int start_o = s->pos, end_o = s->pos + s->target_len; + + // introduce softclip from worst alignment score position to the end (proper one, depending on passed in alignment 'end' for repair and on read strand) + cigar_i = (0 != strand) ? nn_cigar : s->n_cigar - 1 - nn_cigar; + op_len = TMAP_SW_CIGAR_LENGTH (s->cigar [cigar_i]); + if (ind == op_len || ind == 0) + { + if (nn_cigar == s->n_cigar - 1) + return repaired; // all are removed, something not quite right. Give up + if (nn_cigar == s->n_cigar - 2) + { + if (TMAP_SW_CIGAR_OP (s->cigar [cigar_i + inc]) == BAM_CSOFT_CLIP) + return repaired; // something wrong, will become XSYS?? + } + TMAP_SW_CIGAR_STORE (s->cigar [cigar_i], BAM_CSOFT_CLIP, total_scl); + s->n_cigar -= nn_cigar; + } + else + { + TMAP_SW_CIGAR_STORE (s->cigar [cigar_i], BAM_CMATCH, op_len - ind); + if (nn_cigar == 0) + { + s->cigar = tmap_realloc (s->cigar, sizeof (uint32_t) * (1 + s->n_cigar), "s->cigar"); + if (0 != strand) + { + for (i = s->n_cigar - 1; i >= 0; i--) + s->cigar [i+1] = s->cigar [i]; + TMAP_SW_CIGAR_STORE (s->cigar [0], BAM_CSOFT_CLIP, total_scl); + } + else + { + TMAP_SW_CIGAR_STORE (s->cigar [s->n_cigar], BAM_CSOFT_CLIP, total_scl); + } + s->n_cigar++; + } + else + { + cigar_i -= inc; // move back one + TMAP_SW_CIGAR_STORE (s->cigar [cigar_i], BAM_CSOFT_CLIP, total_scl); + s->n_cigar -= nn_cigar - 1; + } + } + // record in stat that we clipped the alignment + stat->num_end_repair_clipped [sori] ++; + stat->bases_end_repair_clipped [sori] += total_scl; + if (0 != strand && cigar_i > 0) + { + for (i = 0; i < s->n_cigar; i++) + { + s->cigar [i] = s->cigar [i+cigar_i]; + } + } + // adjust target position for skipped target bases. DK: Is this right for 5' forward (strand replaced to reverse...)? - yes seems Ok. + if (0 != strand) + s->pos += target_red; + s->target_len -= target_red; + + if (repair5p) + s->fivep_offset = target_red; + + // do one large gap label:oneGapZZ + uint32_t ampl_start, ampl_end; + int min_anchor = opt->min_anchor_large_indel_rescue; + int half_anchor = min_anchor / 2; + if (refseq->bed_exist && total_scl >= half_anchor && + tmap_map_get_amplicon (refseq, s->seqid, start_o, end_o, &l_start, &l_end, o_strand)) + { + //total_scl is desired to be larger than 5 + //fprintf(stderr, "%d %d %d qlen=%d\n", ampl_start, ampl_end, total_scl, qlen); + int max_q = 2; + int original_total_scl = total_scl; + int s_pos_adj = 0; + if (total_scl < min_anchor) + { + total_scl = min_anchor; + s_pos_adj = total_scl - original_total_scl; /* Trimming bases from alignment to get 6 in case of repeat ZZ */ + } + int need_do = 1; + int half_buffer = /*6*/ opt->amplicon_overrun; // outside freedom. + int buffer = half_buffer + 3; + uint32_t start, end; + uint32_t pad_s, pad_e, pad; + pad_s = pad_e = pad = 0; + if (strand == 0) + { + start = s->pos + s->target_len + 1 - s_pos_adj; + end = ampl_end + half_buffer; + pad_s = 100; + if (pad_s > qlen - total_scl) + pad_s = qlen - total_scl; + if (end > refseq->annos [s->seqid].len) + end = refseq->annos [s->seqid].len; + query += qlen-total_scl; + pad = pad_s; + } + else + { + if (ampl_start <= half_buffer) + start = 1; + else + start = ampl_start - half_buffer; + end = s->pos + s_pos_adj; + pad_e = 100; + if (pad_e > qlen - total_scl) + pad_e = qlen - total_scl; + if (pad_e + end > refseq->annos [s->seqid].len) + pad_e = refseq->annos [s->seqid].len - end; + pad = pad_e; + } + tlen = end - start + 1; + //fprintf(stderr, "%d %d \n", start, end); + if (tlen >= min_anchor) + { + if (target_mem < tlen + pad) + { // more memory? + target_mem = tlen + pad; + tmap_roundup32 (target_mem); + target = tmap_realloc (target, sizeof (uint8_t) * target_mem, "target"); + } + if (NULL == tmap_refseq_subseq2 (refseq, s->seqid + 1, start - pad_s, end + pad_e, target, 1, NULL)) + tmap_bug (); + } + else + need_do = 0; + int softclip, target_adj, n_mismatch, indel_size, is_ins, extra_match = 0; + if (need_do) + { + if (tmap_map_util_one_gap (query, // query + total_scl, // q_len + target + pad_s, // target + tlen, // t_len + opt->min_anchor_large_indel_rescue /*anchor size*/, // min_size - a + max_q, // max softclip in query + buffer /*int max_t*/, // max shift on target before M segment + 3 /*maxNM*/, // max allowed mismatches in the resulting structure + opt->max_one_large_indel_rescue, /*int max_indel*/ // max ins / del size + strand, // strand (0 forward 1 reverse) + &n_mismatch, // number of mismatches in resulting structure + &indel_size, // size of the indel in resulting structure + &is_ins, // 1==I, 0==D + &softclip, // size of softclip + pad, // size of pad on either side of target // DK: what if assymetric, like at the end of chromosome? + &extra_match)) // + { + free (target); + //adjust cigar + total_scl = original_total_scl; // adjust back + int match_size; + int target_adj; // how many additional target bases aligned. + if (is_ins) + match_size = total_scl - softclip - indel_size; + else + match_size = total_scl - softclip; + target_adj = match_size; + if (!is_ins) + target_adj += indel_size; + int s_adj = (match_size - n_mismatch) * opt->score_match - n_mismatch * opt->pen_mm - opt->pen_gapo - opt->pen_gape; + s->score += s_adj; + // adjust match size + int inc_cigar = 1; + if (softclip > 0) + inc_cigar++; + int dec_cigar = trim_read_bases (s, extra_match, strand, &is_ins, &indel_size); + if (inc_cigar > dec_cigar) + s->cigar = tmap_realloc (s->cigar, sizeof (uint32_t) * (inc_cigar - dec_cigar + s->n_cigar), "s->cigar"); + match_size += extra_match; + if (strand == 0) + { + if (dec_cigar > 0) + { + s->n_cigar -= dec_cigar; + } + i = s->n_cigar - 1; + if (indel_size > 0) + TMAP_SW_CIGAR_STORE (s->cigar [i++], is_ins ? BAM_CINS : BAM_CDEL, indel_size); + TMAP_SW_CIGAR_STORE (s->cigar [i++], BAM_CMATCH, match_size); + if (softclip > 0) + { + TMAP_SW_CIGAR_STORE (s->cigar [i++], BAM_CSOFT_CLIP, softclip); + } + s->n_cigar = i; + } + else + { + int move = inc_cigar - dec_cigar; + if (indel_size == 0) + move--; + if (move > 0) + { + for (i = s->n_cigar-1; i >= dec_cigar; i--) + s->cigar [i + move] = s->cigar [i]; + } + else if (move < 0) + { + for (i = dec_cigar; i < s->n_cigar; i++) + s->cigar [i + move] = s->cigar [i]; + } + s->n_cigar += move; + i = 0; + if (softclip > 0) + TMAP_SW_CIGAR_STORE (s->cigar [i++], BAM_CSOFT_CLIP, softclip); + TMAP_SW_CIGAR_STORE (s->cigar [i++], BAM_CMATCH, match_size); + if (indel_size > 0) + TMAP_SW_CIGAR_STORE (s->cigar [i++], is_ins? BAM_CINS: BAM_CDEL , indel_size); + //fprintf(stderr, "match_size=%d indel_size=%d is_ins=%d extra_match=%d\n", match_size, indel_size, is_ins, extra_match); + s->pos -= target_adj; + } + s->target_len += target_adj; + if (repair5p) + s->fivep_offset = softclip; // assume here are all mismatches ZZ. + + // DK: quick fix. merge adjacent cigar operations that have the same type + tmap_map_util_merge_adjacent_cigar_operations(s); + + // record stats + stat->num_end_repair_extended [sori] ++; + stat->bases_end_repair_extended [sori] += total_scl - softclip; + stat->total_end_repair_indel [sori] += indel_size; + + repaired = 2; + } + } + } + } + return repaired; + } + // do not perform if so + if (repair5p) + return repaired; + if(1 == softclip_start) + return repaired; + + // check the first cigar op + cigar_i = (0 == strand) ? 0 : (s->n_cigar - 1); + op = TMAP_SW_CIGAR_OP(s->cigar[cigar_i]); + op_len = TMAP_SW_CIGAR_LENGTH(s->cigar[cigar_i]); + if(op != BAM_CMATCH) + return repaired; // ignore, since we care only about turning mismatches into indels + + // get the amount of bases to adjust + cur_len = (max_match_len < op_len) ? max_match_len : op_len; + // NB: must include full HPs + while(cur_len < qlen && query[cur_len-1] == query[cur_len]) + { // TODO: what about reference HPs? + cur_len++; + } + if(cur_len < qlen) + { + cur_len++; // NB: include one more base to get left-justification correct + } - // compute the old score of this sub-alignment - old_score = 0; - // NB: assumes the op is a match/mismatch - found = 0; // did we find mismatches? - if(0 == strand) { // forward - for(i=0;iscore_match; - else old_score -= opt->pen_mm, found = 1; - } - } - else { // reverse - for(i=0;iscore_match; - else old_score -= opt->pen_mm, found = 1; - } - } - if(0 == found) return; // no mismatches, so what are you worrying about? - - // see how many bases we should include from the reference - target_adj = 0; - cur_cur_len = cur_len; - cur_cigar_i = (0 == strand) ? 0 : (s->n_cigar - 1); - cur_op = TMAP_SW_CIGAR_OP(s->cigar[cur_cigar_i]); - cur_op_len = TMAP_SW_CIGAR_LENGTH(s->cigar[cur_cigar_i]); - while(0 < cur_cur_len) { // while read bases - if(0 == cur_op_len) { // update cigar - if(0 == strand) { - cur_cigar_i++; - } - else { - cur_cigar_i--; - } - cur_op = TMAP_SW_CIGAR_OP(s->cigar[cur_cigar_i]); - cur_op_len = TMAP_SW_CIGAR_LENGTH(s->cigar[cur_cigar_i]); - } + // compute the old score of this sub-alignment + old_score = 0; + // NB: assumes the op is a match/mismatch + found = 0; // did we find mismatches? + if(0 == strand) + { // forward + for(i=0;iscore_match; + else + old_score -= opt->pen_mm, found = 1; + } + } + else + { // reverse + for(i=0;iscore_match; + else + old_score -= opt->pen_mm, found = 1; + } + } + if(0 == found) + return repaired; // no mismatches, so what are you worrying about? + + // see how many bases we should include from the reference + target_adj = 0; + cur_cur_len = cur_len; + cur_cigar_i = (0 == strand) ? 0 : (s->n_cigar - 1); + cur_op = TMAP_SW_CIGAR_OP(s->cigar[cur_cigar_i]); + cur_op_len = TMAP_SW_CIGAR_LENGTH(s->cigar[cur_cigar_i]); + while(0 < cur_cur_len) + { // while read bases + if(0 == cur_op_len) + { // update cigar + if(0 == strand) + { + cur_cigar_i++; + } + else + { + cur_cigar_i--; + } + cur_op = TMAP_SW_CIGAR_OP(s->cigar[cur_cigar_i]); + cur_op_len = TMAP_SW_CIGAR_LENGTH(s->cigar[cur_cigar_i]); + } - switch(cur_op) { - case BAM_CMATCH: - cur_cur_len--; - target_adj++; - break; - case BAM_CINS: - cur_cur_len--; - break; - case BAM_CDEL: - target_adj++; - break; - default: - tmap_bug(); - break; - } - cur_op_len--; - } + switch(cur_op) + { + case BAM_CMATCH: + cur_cur_len--; + target_adj++; + break; + case BAM_CINS: + cur_cur_len--; + break; + case BAM_CDEL: + target_adj++; + break; + default: + tmap_bug(); + break; + } + cur_op_len--; + } - // get more target upstream - if(0 == strand) { // forward - start_pos = s->pos + 1; // one-based - if(start_pos < num_prev_bases) start_pos = 1; - else start_pos -= num_prev_bases; - end_pos = s->pos + target_adj; // one-based - } - else { - start_pos = s->pos + s->target_len; // 1-based - if(start_pos <= target_adj) start_pos = 1; - else start_pos -= target_adj - 1; - end_pos = s->pos + s->target_len + num_prev_bases; - if(refseq->annos[s->seqid].len < end_pos) end_pos = refseq->annos[s->seqid].len; // bound - } - tlen = end_pos - start_pos + 1; - if(target_mem < tlen) { // more memory? - target_mem = tlen; - tmap_roundup32(target_mem); - target = tmap_realloc(target, sizeof(uint8_t)*target_mem, "target"); - } - // NB: IUPAC codes are turned into mismatches - if(NULL == tmap_refseq_subseq2(refseq, s->seqid+1, start_pos, end_pos, target, 0, &conv)) { - tmap_bug(); - } + // get more target upstream + if(0 == strand) + { // forward + start_pos = s->pos + 1; // one-based + if(start_pos < num_prev_bases) + start_pos = 1; + else + start_pos -= num_prev_bases; + end_pos = s->pos + target_adj; // one-based + } + else + { + start_pos = s->pos + s->target_len; // 1-based + if(start_pos <= target_adj) + start_pos = 1; + else + start_pos -= target_adj - 1; + end_pos = s->pos + s->target_len + num_prev_bases; + if(refseq->annos[s->seqid].len < end_pos) + end_pos = refseq->annos[s->seqid].len; // bound + } + tlen = end_pos - start_pos + 1; + if(target_mem < tlen) + { // more memory? + target_mem = tlen; + tmap_roundup32(target_mem); + target = tmap_realloc(target, sizeof(uint8_t)*target_mem, "target"); + } + // NB: IUPAC codes are turned into mismatches + if(NULL == tmap_refseq_subseq2(refseq, s->seqid+1, start_pos, end_pos, target, 0, &conv)) + { + tmap_bug(); + } - // set the scoring parameters - if(0 < conv) { - par_iupac.matrix = matrix_iupac; - for(i=0;i<80;i++) { - if(0 < matrix_iupac_mask[i]) (par_iupac).matrix[i] = (opt)->score_match; - else (par_iupac).matrix[i] = -cur_len * (opt)->pen_mm; - } - (par_iupac).row = 16; - (par_iupac).band_width = (opt)->bw; - - (par_iupac).gap_open = 0; - (par_iupac).gap_ext = (opt)->pen_mm; - (par_iupac).gap_end = (opt)->pen_mm; + // set the scoring parameters + if(0 < conv) + { + par_iupac.matrix = matrix_iupac; + for(i=0;i<80;i++) + { + if(0 < matrix_iupac_mask[i]) + (par_iupac).matrix[i] = (opt)->score_match; + else + (par_iupac).matrix[i] = -cur_len * (opt)->pen_mm; + } + (par_iupac).row = 16; + (par_iupac).band_width = (opt)->bw; - } - else { - par.matrix = matrix; - __map_util_gen_ap(par, opt); - for(i=0;i<25;i++) { - (par).matrix[i] = -cur_len * opt->pen_mm; // TODO: is this enough? - } - for(i=0;i<4;i++) { - (par).matrix[i*5+i] = (opt)->score_match; - } - (par).row = 5; - (par).band_width = (opt)->bw; - (par).gap_open = 0; - (par).gap_ext = (opt)->pen_mm; - (par).gap_end = (opt)->pen_mm; - } + (par_iupac).gap_open = 0; + (par_iupac).gap_ext = (opt)->pen_mm; + (par_iupac).gap_end = (opt)->pen_mm; - // adjust query for the reverse strand - if(1 == strand) { // reverse - query = query + qlen - cur_len; // NB: do not use this otherwise - } + } + else + { + par.matrix = matrix; + __map_util_gen_ap(par, opt); + for(i=0;i<25;i++) + { + (par).matrix[i] = -cur_len * opt->pen_mm; // TODO: is this enough? + } + for(i=0;i<4;i++) + { + (par).matrix[i*5+i] = (opt)->score_match; + } + (par).row = 5; + (par).band_width = (opt)->bw; + (par).gap_open = 0; + (par).gap_ext = (opt)->pen_mm; + (par).gap_end = (opt)->pen_mm; + } - /* - for(i=0;iend_repair && old_score == new_score)) { // update - // get the cigar - cigar = tmap_sw_path2cigar(path, path_len, &n_cigar); - if(0 == n_cigar) { - tmap_bug(); - } - // TODO: what if it starts with an indel + /* + for(i=0;i>1;i++) { - int32_t j = cigar[i]; - cigar[i] = cigar[n_cigar-i-1]; - cigar[n_cigar-i-1] = j; - } - } - */ + // map the target against the the query + // path memory + if (*path_buf_sz <= tlen + cur_len) + { // lengthen the path + *path_buf_sz = tlen + cur_len; + tmap_roundup32 (*path_buf_sz); + *path_buf = tmap_realloc (*path_buf, sizeof (tmap_sw_path_t) * *path_buf_sz, "path_buf"); + } - // check if the cigars match - if(1 == n_cigar && TMAP_SW_CIGAR_OP(cigar[0]) == BAM_CMATCH) found = 0; - else found = 1; // do not match, hmmn - } + if(0 == strand) + { + new_score = tmap_sw_clipping_core2(target, tlen, query, cur_len, + (0 < conv) ? &par_iupac : &par, + 1, 0, // do not skip the end of the target + 0, 0, // no soft-clipping + *path_buf, &path_len, 0); + } + else + { + new_score = tmap_sw_clipping_core2(target, tlen, query, cur_len, + (0 < conv) ? &par_iupac : &par, + 0, 1, // do not skip the start of the target + 0, 0, // no soft-clipping + *path_buf, &path_len, 0); + } + //fprintf(stderr, "new_score=%d old_score=%d\n", new_score, old_score); + + found = 0; // reset + if(old_score < new_score || (2 == opt->end_repair && old_score == new_score)) + { // update + // get the cigar + cigar = tmap_sw_path2cigar(*path_buf, path_len, &n_cigar); + if(0 == n_cigar) + { + tmap_bug(); + } + // TODO: what if it starts with an indel + + /* + if(0 == strand) { // reverse the cigar + for(i=0;i>1;i++) { + int32_t j = cigar[i]; + cigar[i] = cigar[n_cigar-i-1]; + cigar[n_cigar-i-1] = j; + } + } + */ - // should we update? - if(1 == found) { - /* - fprintf(stderr, "strand=%d\n", strand); - fprintf(stderr, "cur_len=%d\n", cur_len); - fprintf(stderr, "OLD:\n"); - for(i=0;in_cigar;i++) { // old - fprintf(stderr, "i=%d %d%c\n", i, - bam_cigar_oplen(s->cigar[i]), - bam_cigar_opchr(s->cigar[i])); - } - fprintf(stderr, "ADDITION:\n"); - for(i=0;in_cigar-1; - while((0 == strand && i < s->n_cigar) || (1 == strand && 0 <= i)) { // gets the number to delete, and modifies the last one if necessary in-place - // query - switch(TMAP_SW_CIGAR_OP(s->cigar[i])) { - case BAM_CMATCH: - case BAM_CINS: - sum += bam_cigar_oplen(s->cigar[i]); - default: - break; - } - // target - switch(TMAP_SW_CIGAR_OP(s->cigar[i])) { - case BAM_CMATCH: - case BAM_CDEL: - if(cur_len <= sum) num_ref_removed += (cur_len - (sum - bam_cigar_oplen(s->cigar[i]))); - else num_ref_removed += bam_cigar_oplen(s->cigar[i]); - default: - break; - } + // check if the cigars match + if(1 == n_cigar && TMAP_SW_CIGAR_OP(cigar[0]) == BAM_CMATCH) + found = 0; + else + found = 1; // do not match, hmmn + } - //fprintf(stderr, "i=%d cur_len=%d sum=%d\n", i, cur_len, sum); - if(cur_len <= sum) { - if(cur_len < sum) { // adjust current cigar - TMAP_SW_CIGAR_STORE(s->cigar[i], TMAP_SW_CIGAR_OP(s->cigar[i]), sum - cur_len); // store the difference - i = (0 == strand) ? (i-1) : (i+1); - } - break; - } - i = (0 == strand) ? (i+1) : (i-1); - } - n = (0 == strand) ? (i+1) : (s->n_cigar - i); // the number of cigar operations to delete - //fprintf(stderr, "to delete: %d\n", n); - if(0 < n) { // delete the cigars - if(0 == strand) { // shift down, delete the first cigar operator - for(i=0;in_cigar-n;i++) { - s->cigar[i] = s->cigar[i+n]; - } - } - s->n_cigar -= n; - s->cigar = tmap_realloc(s->cigar, sizeof(uint32_t)*s->n_cigar, "s->cigar"); // reduce the size - } - /* - fprintf(stderr, "CUT DOWN:\n"); - for(i=0;in_cigar;i++) { // old - fprintf(stderr, "i=%d %d%c\n", i, - bam_cigar_oplen(s->cigar[i]), - bam_cigar_opchr(s->cigar[i])); - } - */ - - // get more cigar - s->cigar = tmap_realloc(s->cigar, sizeof(uint32_t)*(n_cigar+s->n_cigar), "s->cigar"); - if(0 == strand) { // forward - // shift up - for(i=s->n_cigar-1;0<=i;i--) { - s->cigar[i+n_cigar] = s->cigar[i]; - } - } // otherwise append to the end - - // add new cigar and update the position - for(i=0;icigar[i] = cigar[i]; - } - else { // reverse - s->cigar[i + s->n_cigar] = cigar[i]; - } - switch(TMAP_SW_CIGAR_OP(cigar[i])) { - case BAM_CMATCH: - case BAM_CDEL: - num_ref_added += TMAP_SW_CIGAR_LENGTH(cigar[i]); - default: - break; - } - /* - switch(TMAP_SW_CIGAR_OP(cigar[i])) { + // should we update? + if(1 == found) + { + /* + fprintf(stderr, "strand=%d\n", strand); + fprintf(stderr, "cur_len=%d\n", cur_len); + fprintf(stderr, "OLD:\n"); + for(i=0;in_cigar;i++) { // old + fprintf(stderr, "i=%d %d%c\n", i, + bam_cigar_oplen(s->cigar[i]), + bam_cigar_opchr(s->cigar[i])); + } + fprintf(stderr, "ADDITION:\n"); + for(i=0;in_cigar-1; + while((0 == strand && i < s->n_cigar) || (1 == strand && 0 <= i)) + { // gets the number to delete, and modifies the last one if necessary in-place + // query + switch(TMAP_SW_CIGAR_OP(s->cigar[i])) + { + case BAM_CMATCH: + case BAM_CINS: + sum += bam_cigar_oplen(s->cigar[i]); + default: + break; + } + // target + switch(TMAP_SW_CIGAR_OP(s->cigar[i])) + { + case BAM_CMATCH: + case BAM_CDEL: + if(cur_len <= sum) num_ref_removed += (cur_len - (sum - bam_cigar_oplen(s->cigar[i]))); + else num_ref_removed += bam_cigar_oplen(s->cigar[i]); + default: + break; + } + + //fprintf(stderr, "i=%d cur_len=%d sum=%d\n", i, cur_len, sum); + if(cur_len <= sum) + { + if(cur_len < sum) + { // adjust current cigar + TMAP_SW_CIGAR_STORE(s->cigar[i], TMAP_SW_CIGAR_OP(s->cigar[i]), sum - cur_len); // store the difference + i = (0 == strand) ? (i-1) : (i+1); + } + break; + } + i = (0 == strand) ? (i+1) : (i-1); + } + n = (0 == strand) ? (i+1) : (s->n_cigar - i); // the number of cigar operations to delete + //fprintf(stderr, "to delete: %d\n", n); + if(0 < n) + { // delete the cigars + if(0 == strand) + { // shift down, delete the first cigar operator + for(i=0;in_cigar-n;i++) + { + s->cigar[i] = s->cigar[i+n]; + } + } + s->n_cigar -= n; + s->cigar = tmap_realloc(s->cigar, sizeof(uint32_t)*s->n_cigar, "s->cigar"); // reduce the size + } + /* + fprintf(stderr, "CUT DOWN:\n"); + for(i=0;in_cigar;i++) { // old + fprintf(stderr, "i=%d %d%c\n", i, + bam_cigar_oplen(s->cigar[i]), + bam_cigar_opchr(s->cigar[i])); + } + */ + + // get more cigar + s->cigar = tmap_realloc(s->cigar, sizeof(uint32_t)*(n_cigar+s->n_cigar), "s->cigar"); + if(0 == strand) + { // forward + // shift up + for(i=s->n_cigar-1;0<=i;i--) + { + s->cigar[i+n_cigar] = s->cigar[i]; + } + } // otherwise append to the end + + // add new cigar and update the position + for(i=0;icigar[i] = cigar[i]; + } + else + { // reverse + s->cigar[i + s->n_cigar] = cigar[i]; + } + switch(TMAP_SW_CIGAR_OP(cigar[i])) + { + case BAM_CMATCH: + case BAM_CDEL: + num_ref_added += TMAP_SW_CIGAR_LENGTH(cigar[i]); + default: + break; + } + /* + switch(TMAP_SW_CIGAR_OP(cigar[i])) { case BAM_CDEL: - s->target_len -= TMAP_SW_CIGAR_LENGTH(cigar[i]); - if(0 == strand) s->pos -= TMAP_SW_CIGAR_LENGTH(cigar[i]); - break; + s->target_len -= TMAP_SW_CIGAR_LENGTH(cigar[i]); + if(0 == strand) s->pos -= TMAP_SW_CIGAR_LENGTH(cigar[i]); + break; case BAM_CINS: - s->target_len += TMAP_SW_CIGAR_LENGTH(cigar[i]); - if(0 == strand) s->pos += TMAP_SW_CIGAR_LENGTH(cigar[i]); - break; + s->target_len += TMAP_SW_CIGAR_LENGTH(cigar[i]); + if(0 == strand) s->pos += TMAP_SW_CIGAR_LENGTH(cigar[i]); + break; default: - break; - } - */ - } - s->n_cigar += n_cigar; - s->target_len += (num_ref_added - num_ref_removed); - if(0 == strand) s->pos += (num_ref_removed - num_ref_added); + break; + } + */ + } + s->n_cigar += n_cigar; + s->target_len += (num_ref_added - num_ref_removed); + if(0 == strand) + s->pos += (num_ref_removed - num_ref_added); + + // merge adjacent cigar operations that have the same value + tmap_map_util_merge_adjacent_cigar_operations(s); + /* + for(i=0;in_cigar;i++) { + fprintf(stderr, "i=%d %d%c\n", i, + bam_cigar_oplen(s->cigar[i]), + bam_cigar_opchr(s->cigar[i])); + } + */ + + // update the score + // TODO: is this correct? + s->score += new_score; + s->score -= old_score; + + /* + fprintf(stderr, "NEW:\n"); + for(i=0;in_cigar;i++) { // old + fprintf(stderr, "i=%d %d%c\n", i, + bam_cigar_oplen(s->cigar[i]), + bam_cigar_opchr(s->cigar[i])); + } + */ + // Check that the cigar is valid + for(i=cur_len=0;in_cigar;i++) + { + switch(TMAP_SW_CIGAR_OP(s->cigar[i])) + { // NB: do not include soft-clip bases + case BAM_CMATCH: + case BAM_CINS: + cur_len += TMAP_SW_CIGAR_LENGTH(s->cigar[i]); + default: + break; + } + } + //fprintf(stderr, "cur_len=%d qlen=%d\n", cur_len, qlen); + if(cur_len != qlen) + tmap_bug(); + } - // merge adjacent cigar operations that have the same value - tmap_map_util_merge_adjacent_cigar_operations(s); - /* - for(i=0;in_cigar;i++) { - fprintf(stderr, "i=%d %d%c\n", i, - bam_cigar_oplen(s->cigar[i]), - bam_cigar_opchr(s->cigar[i])); - } - */ + // free + free(cigar); + free(target); + return repaired; +} - // update the score - // TODO: is this correct? - s->score += new_score; - s->score -= old_score; +void target_cache_init (ref_buf_t* target) +{ + target->buf = NULL; + target->buf_sz = 0; + target->data = NULL; + target->data_len = 0; + target->seqid = 0; // for reference access the sequence ids are 1-based, so 0 is always invalid + target->seq_start = 0xFFFFFFFF; + target->seq_end = 0xFFFFFFFF; +} +void target_cache_free (ref_buf_t* target) +{ + free (target->buf); + target_cache_init (target); +} - /* - fprintf(stderr, "NEW:\n"); - for(i=0;in_cigar;i++) { // old - fprintf(stderr, "i=%d %d%c\n", i, - bam_cigar_oplen(s->cigar[i]), - bam_cigar_opchr(s->cigar[i])); - } - */ - // Check that the cigar is valid - for(i=cur_len=0;in_cigar;i++) { - switch(TMAP_SW_CIGAR_OP(s->cigar[i])) { // NB: do not include soft-clip bases - case BAM_CMATCH: - case BAM_CINS: - cur_len += TMAP_SW_CIGAR_LENGTH(s->cigar[i]); - default: - break; - } - } - //fprintf(stderr, "cur_len=%d qlen=%d\n", cur_len, qlen); - if(cur_len != qlen) tmap_bug(); - } - - // free - free(cigar); - free(target); -} - -void -tmap_map_rmquerybases_cigar(tmap_map_sam_t *s, uint32_t left_rm_bases, uint32_t right_rm_bases) +void cache_target (ref_buf_t* target, tmap_refseq_t *refseq, uint32_t seqid, uint32_t seq_start, uint32_t seq_end) { - - + uint32_t tlen = seq_end - seq_start + 1; + // check if already in buffer + if (seqid == target->seqid && seq_start >= target->seq_start && seq_end <= target->seq_end) + { + target->data = target->buf + (seq_start - target->seq_start); + target->data_len = tlen; + } + else + { + if (target->buf_sz < tlen) + { // more memory? + target->buf_sz = tlen; + tmap_roundup32 (target->buf_sz); + target->buf = tmap_realloc (target->buf, sizeof (uint8_t) * (target->buf_sz), "target->buf"); + } + if (!tmap_refseq_subseq2 (refseq, seqid, seq_start, seq_end, target->buf, 1, NULL)) + { + uint32_t rl = refseq->annos[seqid-1].len; + tmap_progress_print2 ("\n seqid = %d, seq_start = %d, seq_end = %d, Ref seq len = %d", seqid, seq_start, seq_end, rl); + tmap_bug (); + } + target->seqid = seqid; + target->seq_start = seq_start; + target->seq_end = seq_end; + target->data = target->buf; + target->data_len = tlen; + } } -tmap_map_sams_t * -tmap_map_util_sw_gen_cigar(tmap_refseq_t *refseq, - tmap_map_sams_t *sams, - tmap_seq_t *seq, - tmap_seq_t **seqs, - tmap_map_opt_t *opt) -{ - int32_t i, j, matrix[25], matrix_iupac[80]; - // int32_t start, end; - int32_t end; - tmap_map_sams_t *sams_tmp = NULL; - tmap_sw_param_t par, par_iupac; - tmap_sw_path_t *path = NULL; - int32_t path_len, path_mem=0; - int32_t seq_len=0, tlen, target_mem=0; - uint8_t *target=NULL; - tmap_vsw_t *vsw = NULL; - tmap_vsw_opt_t *vsw_opt = NULL; - uint32_t start_pos, end_pos; - int32_t overflow, softclip_start, softclip_end; - uint8_t key_base = 0; - int32_t iupac_init = 0; - - if(0 == sams->n) { - return sams; - } +int find_alignment_start +( + tmap_map_sam_t* src_sam, // source: raw (position-only) mapping + tmap_map_sam_t* dest_sam, // destination: refined (aligned) mapping + tmap_seq_t** seqs, // array of size 4 that contains pre-computed inverse / complement combinations + tmap_refseq_t* refseq, // reference server + int32_t softclip_start, // is 5' softclip allowed + int32_t softclip_end, // is 3' softclip allowed + tmap_map_opt_t* opt, // tmap parameters + ref_buf_t* target, // reference data + tmap_vsw_t *vsw, // vectorized aligner object + tmap_map_stats_t * stat // statistics +) +// returns 1 on success, 0 on failure +{ + // get the target sequence + uint32_t start_pos = src_sam->pos + 1; + uint32_t end_pos = start_pos + src_sam->target_len - 1; + // int32_t tlen = src_sam->result.target_end + 1; // adjust based on the target end + uint8_t* orig_query = (uint8_t*) tmap_seq_get_bases (seqs [src_sam->strand])->s; // forward genomic strand + int32_t orig_query_len = tmap_seq_get_bases_length (seqs [0]); + + // cache data + cache_target (target, refseq, src_sam->seqid + 1, start_pos, end_pos); + + // retrieve the reverse complimented query sequence + uint8_t *query_rc = (uint8_t*) tmap_seq_get_bases (seqs [1])->s; + query_rc += orig_query_len - src_sam->result.query_end - 1; // offset query + + // uint8_t *query_rc = NULL; + int32_t qlen = src_sam->result.query_end + 1; // adjust based on the query end; + // do not band when generating the cigar + tmap_map_sam_t tmp_sam; // TODO: actually, only the .pos, .score and .result members change; it would be cleaner to use only them + tmp_sam = *src_sam; + + // save the position and target len as passed from mapper for end_repair + tmp_sam.mapper_pos = tmp_sam.pos; + tmp_sam.mapper_tlen = tmp_sam.target_len; + + // reverse compliment the target_buf + if(0 == src_sam->strand) + tmap_reverse_compliment_int (target->data, target->data_len); + + // NB: if match/mismatch penalties are on the opposite strands, we may + // have wrong scores + // NB: this aligns in the opposite direction than sequencing (3'->5') + int32_t overflow; + tmp_sam.score = tmap_vsw_process_rev (vsw, query_rc, qlen, target->data, target->data_len, + &tmp_sam.result, &overflow, opt->score_thr, + (1 == softclip_end && 1 == softclip_start) ? 0 : 1); // NB: to guarantee correct soft-clipping if both ends are clipped + + if(1 == overflow) + tmap_bug(); + + // reverse back compliment the target_buf + if(0 == tmp_sam.strand) + tmap_reverse_compliment_int (target->data, target->data_len); + + if (tmp_sam.score < opt->score_thr) // this could happen if VSW fails. Just skip the damned, with no warning ?!? + return 0; // TODO: may beed warning or error report here + + if (0 == tmp_sam.strand) + { + tmp_sam.pos += tmp_sam.result.target_start; // keep it zero based + } + else + { + int32_t query_start = orig_query_len - tmp_sam.result.query_end - 1; + int32_t query_end = orig_query_len - tmp_sam.result.query_start - 1; + tmp_sam.result.query_start = query_start; + tmp_sam.result.query_end = query_end; + } + tmp_sam.result.target_end -= tmp_sam.result.target_start; + tmp_sam.result.target_start = 0; - // the final mappings will go here - sams_tmp = tmap_map_sams_init(sams); - tmap_map_sams_realloc(sams_tmp, sams->n); - // scoring matrix - par.matrix = matrix; - __map_util_gen_ap(par, opt); + *dest_sam = tmp_sam; - // sort by strand/chr/pos/score - tmap_sort_introsort(tmap_map_sam_sort_coord, sams->n, sams->sams); - tmap_map_util_set_softclip(opt, seq, &softclip_start, &softclip_end); - if (0 && softclip_end == 0 && refseq->bed_exist) { - // ZZ: only disable 3' end softclip if the read aligned all the way close to the end of amplicon - end = 0; - while(end < sams->n) { - uint8_t strand; // *query=NULL, *query_rc=NULL, *tmp_target=NULL; - // int32_t qlen; - tmap_map_sam_t tmp_sam; - // int32_t query_start, query_end; - // int32_t conv = 0; - - // do not band when generating the cigar - tmp_sam = sams->sams[end]; - // get the strand/start/end positions - strand = tmp_sam.strand; - start_pos = tmp_sam.pos + 1; - end_pos = start_pos + tmp_sam.target_len - 1; - uint32_t ampl_start, ampl_end; - if (tmap_map_get_amplicon(refseq, tmp_sam.seqid, start_pos, end_pos, &l_start, &l_end, strand)) { - if (strand == 0 && abs(ampl_end-end_pos) < 15) break; - if (strand != 0 && abs(ampl_start-start_pos) < 15) break; - } - end++; + // update aux data + tmap_map_sam_malloc_aux (dest_sam); + switch (dest_sam->algo_id) + { + case TMAP_MAP_ALGO_MAP1: + (*dest_sam->aux.map1_aux) = (*tmp_sam.aux.map1_aux); + break; + case TMAP_MAP_ALGO_MAP2: + (*dest_sam->aux.map2_aux) = (*tmp_sam.aux.map2_aux); + break; + case TMAP_MAP_ALGO_MAP3: + (*dest_sam->aux.map3_aux) = (*tmp_sam.aux.map3_aux); + break; + case TMAP_MAP_ALGO_MAP4: + (*dest_sam->aux.map4_aux) = (*tmp_sam.aux.map4_aux); + break; + case TMAP_MAP_ALGO_MAPVSW: + (*dest_sam->aux.map_vsw_aux) = (*tmp_sam.aux.map_vsw_aux); + break; + default: + tmap_bug (); + break; } - if (end >= sams->n) softclip_end = 1; - } - - // initialize opt - vsw_opt = tmap_vsw_opt_init(opt->score_match, opt->pen_mm, opt->pen_gapo, opt->pen_gape, opt->score_thr); - - // init seqs - seq_len = tmap_seq_get_bases_length(seqs[0]); + return 1; +} - // reverse compliment query - vsw = tmap_vsw_init((uint8_t*)tmap_seq_get_bases(seqs[1])->s, seq_len, softclip_end, softclip_start, opt->vsw_type, vsw_opt); - - if(1 == opt->softclip_key) { - // get first base - if(NULL == seq->ks) { - key_base = INT8_MAX; - } - else { - key_base = tmap_nt_char_to_int[(uint8_t)seq->ks[strlen(seq->ks)-1]]; // NB: last base of the key - } - } - - // i = start = end = 0; - i = end = 0; - start_pos = end_pos = 0; - while(end < sams->n) { - uint8_t strand, *query=NULL, *query_rc=NULL, *tmp_target=NULL; - int32_t qlen; - tmap_map_sam_t tmp_sam; - tmap_map_sam_t *s = NULL; - int32_t query_start, query_end; - int32_t conv = 0; - - // do not band when generating the cigar - tmp_sam = sams->sams[end]; +static void extend_softclips_to_read_edges ( + tmap_map_sam_t* dest_sam, // mapping being adjusted + tmap_seq_t** seqs // 4-element array containing fwd, rec, compl and rev/compl read sequence +) +{ + int32_t orig_query_len = tmap_seq_get_bases_length (seqs [0]); + + // add needed soft-clips (may be adjusted by salvage) + // Already converted in place, in sams.result (by find_alignment_start) + // int32_t query_start = dest_sam->strand?(orig_query_len - dest_sam->result.query_end - 1):dest_sam->result.query_start; + // int32_t query_end = dest_sam->strand?(orig_query_len - dest_sam->result.query_start - 1):dest_sam->result.query_end; + + // add soft clipping + if(0 < dest_sam->result.query_start) + { + int32_t j; + // soft clip the front of the read + dest_sam->cigar = tmap_realloc (dest_sam->cigar, sizeof (uint32_t) * (1 + dest_sam->n_cigar), "dest_sam->cigar"); + for (j = dest_sam->n_cigar - 1; 0 <= j; j--) // shift up + dest_sam->cigar [j + 1] = dest_sam->cigar [j]; + TMAP_SW_CIGAR_STORE (dest_sam->cigar [0], BAM_CSOFT_CLIP, dest_sam->result.query_start); + dest_sam->n_cigar++; + } + if (dest_sam->result.query_end < orig_query_len - 1) + { + // soft clip the end of the read + dest_sam->cigar = tmap_realloc (dest_sam->cigar, sizeof (uint32_t) * (1 + dest_sam->n_cigar), "dest_sam->cigar"); + TMAP_SW_CIGAR_STORE (dest_sam->cigar [dest_sam->n_cigar], BAM_CSOFT_CLIP, orig_query_len - dest_sam->result.query_end - 1); + dest_sam->n_cigar++; + } +} - // get the strand/start/end positions - strand = tmp_sam.strand; - start_pos = tmp_sam.pos + 1; - end_pos = start_pos + tmp_sam.target_len - 1; - uint32_t left_added_bases = 0, right_added_bases = 0; // ZZ to be removed - - /** - * Step 1: find the start of the alignment - */ - - // retrieve the reverse complimented query sequence - query_rc = (uint8_t*)tmap_seq_get_bases(seqs[1])->s; - query_rc += seq_len - tmp_sam.result.query_end - 1; // offset query - qlen = tmp_sam.result.query_end + 1; // adjust based on the query end - - // get the target sequence - tlen = tmp_sam.result.target_end + 1; // adjust based on the target end - if(target_mem < tlen) { // more memory? - target_mem = tlen; - tmap_roundup32(target_mem); - target = tmap_realloc(target, sizeof(uint8_t)*target_mem, "target"); - } - // NB: IUPAC codes are turned into mismatches - if(NULL == tmap_refseq_subseq2(refseq, sams->sams[end].seqid+1, start_pos, end_pos, target, 1, &conv)) { - tmap_bug(); - } +void compute_alignment ( + tmap_map_sam_t* dest_sam, // mapping to update with cigar + tmap_seq_t** seqs, // array of size 4 that contains pre-computed inverse / complement combinations + tmap_refseq_t* refseq, // reference server + ref_buf_t* target, // reference data + tmap_sw_path_t** path_buf, // buffer for traceback path + int32_t* path_buf_sz, // used portion and allocated size of traceback path. + tmap_sw_param_t* par, // Smith-Waterman scoring parameters + tmap_map_stats_t* stat // statistics +) +{ + uint8_t* orig_query = (uint8_t*) tmap_seq_get_bases (seqs [dest_sam->strand])->s; // forward genomic strand + int32_t orig_query_len = tmap_seq_get_bases_length (seqs [0]); + // uint8_t* adjusted_query = orig_query + (dest_sam->strand?(orig_query_len - dest_sam->result.query_end - 1):(dest_sam->result.query_start)); // offset query + uint8_t* adjusted_query = orig_query + dest_sam->result.query_start; // offset query; the result.query_start is already properly adjusted depending on a strand + + // uint8_t* adjusted_target = (*target_buf) + (dest_sam->strand?0:dest_sam->result.target_start); + uint32_t start_pos = dest_sam->pos + dest_sam->result.target_start + 1; // addition of result.target_start is obsolete, as it always should be reset to 0 by find_alignment_start + uint32_t end_pos = dest_sam->pos + dest_sam->result.target_end + 1; + // cache data + cache_target (target, refseq, dest_sam->seqid + 1, start_pos, end_pos); + + + int32_t qlen = dest_sam->result.query_end - dest_sam->result.query_start + 1; // update query length + // int32_t tlen = dest_sam->result.target_end - dest_sam->result.target_start + 1; + int32_t path_len; + int32_t new_score; + + // path memory + if (*path_buf_sz <= target->data_len + orig_query_len) + { // lengthen the path + *path_buf_sz = target->data_len + orig_query_len; + tmap_roundup32 (*path_buf_sz); + *path_buf = tmap_realloc (*path_buf, sizeof (tmap_sw_path_t) * *path_buf_sz, "path_buf"); + } - // reverse compliment the target - if(0 == strand) { - tmap_reverse_compliment_int(target, tlen); - } + // Smith Waterman with banding + // NB: we store the score from the banded version, which does not allow ins then del, or del then ins. The vectorized version does. + // NB: left genomic indel justification is facilitated by always using the forward strand target/query combination. + new_score = tmap_sw_global_banded_core (target->data, target->data_len, adjusted_query, qlen, par, + dest_sam->result.score_fwd, *path_buf, &path_len, 0); + + // ZZ: There can be case when the global score is the same as the vectorized version + // whileas the alignments are different. We are not addressing all of them, but the + // cases where the resulting alignment starts or ends with deletion shall be avoided. + if ( (new_score != dest_sam->score && 1 < dest_sam->result.n_best) + || (path_len > 0 && (TMAP_SW_FROM_D == (*path_buf) [0].ctype || TMAP_SW_FROM_D == (*path_buf) [path_len - 1].ctype))) + // explicitly fit the query into the target + new_score = tmap_sw_fitting_core (target->data, target->data_len, adjusted_query, qlen, par, *path_buf, &path_len, 0); + // update score + dest_sam->score = new_score; + // adjust position to forward alignment + int32_t path_ref_off = (*path_buf) [path_len - 1].i - 1; // path_ref_off is zero-based + dest_sam->result.target_start += path_ref_off; // target_start is zero-based, path coords are one-based + dest_sam->pos += dest_sam->result.target_start; // move pos to the (updated) target_start + + if ((*path_buf) [path_len-1].ctype == TMAP_SW_FROM_I) // don't quite understand this ZZ + dest_sam->pos++; // if alignment starts with INS in query, this ins is placed at NEXT base in rev reference - alignment start should be one base to the right DK + + + // actually convert SW path to cigar + dest_sam->cigar = tmap_sw_path2cigar (*path_buf, path_len, &(dest_sam->n_cigar)); + if (0 == dest_sam->n_cigar) + tmap_bug (); + + // compute target (alignment on the reference) len + dest_sam->target_len = 0; + uint32_t* cigar_pos; + int32_t j; + for (j = 0, cigar_pos = dest_sam->cigar; j != dest_sam->n_cigar; ++j, ++cigar_pos) + { + if (bam_cigar_type (bam_cigar_op (*cigar_pos)) & 2) + dest_sam->target_len += bam_cigar_oplen (*cigar_pos); + } - // NB: if match/mismatch penalties are on the opposite strands, we may - // have wrong scores - // NB: this aligns in the opposite direction than sequencing (3'->5') - tmp_sam.score = tmap_vsw_process_rev(vsw, query_rc, qlen, target, tlen, - &tmp_sam.result, &overflow, opt->score_thr, - (1 == softclip_end && 1 == softclip_start) ? 0 : 1); // NB: to guarantee correct soft-clipping if both ends are clipped + // from now on, the box is not used in original code - all positions are based on pos and target_len for target, and softclips for query + // we use it for everything, so we'll update the box here + // The query positions is in the direction of strands aligning with FORWARD target: + // : q_start is from the beginning of REVERSE query for reverse matches + // and from the beginning of FORWARD query for fwd matches + // target always mean same + dest_sam->result.query_end = dest_sam->result.query_start + (*path_buf) [0].j - 1; // TODO: check if adjustment for DEL at the alignment end needed + dest_sam->result.query_start += (*path_buf) [path_len - 1].j - 1; // one-based in path, zero-based in box + if ((*path_buf) [path_len-1].ctype == TMAP_SW_FROM_D) // should this ever happen? + dest_sam->result.query_start++; + + dest_sam->result.target_start = 0; + dest_sam->result.target_end = dest_sam->target_len - 1; // TODO: check if adjustment for INS at the alignment end needed + + // cure softclips + extend_softclips_to_read_edges (dest_sam, seqs); + + // remember original alignment + dest_sam->n_orig_cigar = dest_sam->n_cigar; + dest_sam->orig_cigar = tmap_calloc (dest_sam->n_orig_cigar, sizeof (*(dest_sam->orig_cigar)), "orig_cigar"); + dest_sam->orig_pos = dest_sam->pos; + memcpy (dest_sam->orig_cigar, dest_sam->cigar, dest_sam->n_orig_cigar * sizeof (*(dest_sam->orig_cigar))); +} - if(1 == overflow) { - tmap_bug(); - } - - // reverse back compliment the target - if(0 == strand) { - tmap_reverse_compliment_int(target, tlen); - } +void salvage_long_indel_at_edges ( + tmap_map_sam_t* dest_sam, // destination: refined (aligned) mapping + tmap_seq_t** seqs, // array of size 4 that contains pre-computed inverse / complement combinations + tmap_refseq_t* refseq, // reference server + ref_buf_t* target, // reference data + tmap_sw_path_t** path_buf, // buffer for traceback path + int32_t* path_buf_sz, // used portion and allocated size of traceback path. + tmap_map_opt_t* opt, // tmap options + tmap_sw_param_t* par, // Smith-Waterman scopring parameters + tmap_map_stats_t *stat // statistics +) +{ + // long indel prefixes and suffixes + // needs: + // tmap_map_sam_t* dest_sam, // destination: refined (aligned) mapping + // tmap_map_opt_t* opt, // tmap parameters + // tmap_seq_t** seqs, // array of size 4 that contains pre-computed inverse / complement combinations + // tmap_refseq_t* refseq, // reference server + // uint8_t** target_buf, // pointer to the address of the memory buffer for unpacked reference sequence + // int32_t* target_buf_sz, // pointer to the variable contining presently allocated size of target_buf + // tmap_sw_path_t *path_buf, // buffer for traceback path + // int32_t path_buf_sz // used portion and allocated size of traceback path. + // tmap_map_opt_t* opt // tmap options + // tmap_sw_param_t* par; // Smith-Waterman scopring parameters + + uint32_t ampl_start = 0, ampl_end = 0; + uint32_t ampl_exist = 0; + + uint8_t* orig_query = (uint8_t*) tmap_seq_get_bases (seqs [dest_sam->strand])->s; // forward genomic strand + int32_t orig_query_len = tmap_seq_get_bases_length (seqs [0]); + + int32_t query_start = dest_sam->result.query_start; + int32_t query_end = dest_sam->result.query_end; + + // DK: The code below is derived from original Niels's edge indel salvage code + // It assumes cigar string is stripped of softclips. + // We need to save them and then put back. + unsigned beg_softclip = 0, end_softclip = 0; + // int8_t cigar_buf_reallocated = 0; + if (dest_sam->n_cigar > 1 && bam_cigar_op (dest_sam->cigar [dest_sam->n_cigar - 1]) == BAM_CSOFT_CLIP) + { + end_softclip = bam_cigar_oplen (dest_sam->cigar [dest_sam->n_cigar - 1]); + if (0 == end_softclip) + tmap_bug (); + dest_sam->n_cigar --; + } + if (dest_sam->n_cigar && bam_cigar_op (dest_sam->cigar [0]) == BAM_CSOFT_CLIP) + { + beg_softclip = bam_cigar_oplen (dest_sam->cigar [0]); + if (0 == beg_softclip) + tmap_bug (); + dest_sam->n_cigar --; + memmove (dest_sam->cigar, dest_sam->cigar + 1, dest_sam->n_cigar * sizeof (uint32_t)); + } - if(tmp_sam.score < opt->score_thr) { // this could happen if VSW fails - end++; - continue; - } - - // ZZ, figure out amplicon condition to add primers or similar - uint32_t ampl_start = 0, ampl_end = 0; - uint32_t start_pos_a = start_pos, end_pos_a = end_pos; - if (strand == 0) { - start_pos_a += tmp_sam.result.target_start; - } else { - end_pos_a = start_pos+ (tmp_sam.result.target_end-tmp_sam.result.target_start); - } - if (0 && softclip_end == 0 && tmap_map_get_amplicon(refseq, tmp_sam.seqid, start_pos_a, end_pos_a, &l_start, &l_end, strand)) { - uint32_t ds = abs(start_pos_a-ampl_start); - uint32_t de = abs(end_pos_a-ampl_end); - if (ds < 10 && de < 10 && (de > 2 || ds > 2)) { - //if ((strand == 0 && ds < 10 && start_pos_a >= ampl_start+2) || (strand != 0 && de < 10 && end_pos_a <= ampl_end-2)) { - //if ((strand == 0 && ds < 10) || (strand != 0 && de < 10)) { - //int left = 1, right = 1; - //if (strand == 0) left = 0; else right = 0; - //if (ds < 10 && de < 10 /*&& start_pos_a >= ampl_start+2 && end_pos_a <= ampl_end-2*/) left = right = 0; - // target mem - int pad = 20; - tlen = ampl_end-ampl_start+1+pad*2; - if(target_mem < tlen) { // more memory? - target_mem = tlen; - tmap_roundup32(target_mem); - target = tmap_realloc(target, sizeof(uint8_t)*target_mem, "target"); - } - query = (uint8_t*)tmap_seq_get_bases(seqs[strand])->s; - // Get the new target - // NB: IUPAC codes are turned into mismatches - s = &sams_tmp->sams[i]; - (*s) = tmp_sam; - - if(NULL == tmap_refseq_subseq2(refseq, s->seqid+1, ampl_start-pad, ampl_end+pad, target, 0, &conv)) { - tmap_bug(); - } - // finding the digestion sites. - int x; - for (x = tlen-pad; x < tlen; x++) { - if (target[x] == 0) break; - } - x--; - ampl_end = x+(ampl_start-pad); - - for (x = pad-2; x >=0; x--) { - if (target[x] == 3) break; - } - x++; - tmp_target = target; - target += x; - ampl_start = ampl_start-pad+x; - tlen = ampl_end-ampl_start+1; - if(0 < conv && 0 == iupac_init) { - par_iupac.matrix = matrix_iupac; - __map_util_gen_ap_iupac(par_iupac, opt); - iupac_init = 1; - } + /** + * Try to detect large indels present as soft-clipped prefixes of the + * alignment. + */ - // path memory - if(path_mem <= tlen + seq_len) { // lengthen the path - path_mem = tlen + seq_len; - tmap_roundup32(path_mem); - path = tmap_realloc(path, sizeof(tmap_sw_path_t)*path_mem, "path"); - } + uint32_t salvaged = 0; + while(1) // NB: so we can break out at any time + { + if (0 < query_start) // beginning of the alignment (3' for reverse, 5' for forward strand) + { // start of the alignment + // ZZ:We may check amplicon here as well. May try this only when it is near start of an amplicon. + // Here the reference mapping position is known by s->pos to s->pos+s->target_len + // We can check the amplicon with these. + // A. When bed file is not present, current logic holds. + // B.i. If bed file is given, when an amplicon is present, try use all the bases to the begin/end of amplicon, very small or no long gap penalty. + // B.ii.If bed file is given and the hit position is not in an amplicon, normal logic apply. + + int32_t del_score = 0; + int32_t ins_score = 0; + uint32_t op, op_len; + int32_t new_score; + uint32_t *cigar = NULL; + int32_t n_cigar; + int32_t pos_adj = 0; + int32_t start_pos, end_pos; + int32_t path_len; + + // query = (uint8_t*)tmap_seq_get_bases(seqs[tmp_sam.strand])->s; // forward genomic strand + // DK: this is thr orig_query + + // get the target sequence before the start of the alignment + int32_t tglen = query_start + opt->gapl_len; + + if (dest_sam->pos <= tglen) + start_pos = 1; + else + start_pos = dest_sam->pos - tglen + 1; + end_pos = dest_sam->pos; + if (end_pos < start_pos) + break; // exit the loop + int32_t long_gap = opt->pen_gapl; + //ZZ: check amplicon + uint32_t ampl_start = 0, ampl_end = 0; + if (tmap_map_get_amplicon (refseq, dest_sam->seqid, dest_sam->pos, dest_sam->pos + dest_sam->target_len, &l_start, &l_end, dest_sam->strand)) + { + ampl_exist = 1; + if (ampl_start >= dest_sam->pos) + break; + if (start_pos > ampl_start) + start_pos = ampl_start; + long_gap = 0; + } + + // DK - do not reset; use target_buf, it is kept intact + // target mem + // target = tmp_target; // reset target in memory + cache_target (target, refseq, dest_sam->seqid + 1, start_pos, end_pos); + + // try to add a larger deletion + del_score = tmap_sw_clipping_core2 (target->data, target->data_len, orig_query, query_start, par, + 1, 1, // allow the start and end of the target to be skipped + 1, 0, // deletions + NULL, 0, 0); - s->score = 20*opt->score_match+ tmap_sw_clipping_core2(target, tlen, query, seq_len, - (0 < conv) ? &par_iupac : &par, - 0, 0, //whole amplicon must be aligned ZZ - 1, 1, /*0, 0,*/ // whole global, may use a better algorithm ZZ - path, &path_len, 0); - //fprintf(stderr, "ZZ= score=%d %d seqid%d %d %d %d %d %d %d strand=%d %d %d\n", s->score, tmp_sam.score, s->seqid+1, ampl_start, ampl_end, start_pos_a, end_pos_a, tmp_sam.result.target_start, tmp_sam.result.target_end, strand, start_pos, end_pos); - //if (s->score > tmp_sam.score) { - int tail_clip = seq_len-path[0].j; - int begin_clip = path[path_len-1].j-1; - if (TMAP_SW_FROM_D == path[path_len-1].ctype) begin_clip++; - s->cigar = tmap_sw_path2cigar(path, path_len, &s->n_cigar); - // need to add soft clip, possibly on both sides - if(0 == s->n_cigar) { - tmap_bug(); - } - s->pos = ampl_start-1; - s->target_len = tlen; - int f = (begin_clip >0) ? 1:0; - int e = (tail_clip > 0) ? 1:0; - if (f+e > 0) { - s->cigar = tmap_realloc(s->cigar, sizeof(uint32_t)*(f+e+s->n_cigar), "s->cigar"); - if (f > 0) { - int i; - for (i = s->n_cigar-1; i>=0; i--) { - s->cigar[i+1] = s->cigar[i]; - } - TMAP_SW_CIGAR_STORE(s->cigar[0], BAM_CSOFT_CLIP, begin_clip); - } - if (e > 0) TMAP_SW_CIGAR_STORE(s->cigar[s->n_cigar+f+e-1], BAM_CSOFT_CLIP, tail_clip); - s->n_cigar += e+f; - } - // check query length and cigar - int tttlen = 0; - for (j = 0; j < s->n_cigar; j++) { - switch(TMAP_SW_CIGAR_OP(s->cigar[j])) { + // try to add a larger insertion + // TODO: how do we enforce that the target starts immediately where + // we want it to? + ins_score = tmap_sw_clipping_core2 (target->data, target->data_len, orig_query, query_start, par, + 1, 0, // only allow the start of the target to be skipped + 1, 1, // insertions, + NULL, 0, 0); + // reset start/end position + start_pos = dest_sam->pos + 1; + end_pos = dest_sam->pos + dest_sam->target_len; + + if (del_score <= 0 && ins_score <= 0) + // do nothing + new_score = -1; + else + { + // path memory + if (*path_buf_sz <= target->data_len + query_start) + { // lengthen the path + *path_buf_sz = target->data_len + query_start; + tmap_roundup32 (*path_buf_sz); + *path_buf = tmap_realloc (*path_buf, sizeof (tmap_sw_path_t) * *path_buf_sz, "path_buf"); + } + if (del_score < ins_score) + { + // get the path + tmap_sw_clipping_core2 (target->data, target->data_len, orig_query, query_start, + par, + 1, 0, // only allow the start of the target to be skipped + 1, 1, // insertions, + *path_buf, &path_len, 0); + op = BAM_CINS; + op_len = query_start - (*path_buf) [0].j; + new_score = ins_score; + pos_adj = 0; + } + else + { + // get the path + tmap_sw_clipping_core2 (target->data, target->data_len, orig_query, query_start, + par, + 1, 1, // allow the start and end of the target to be skipped + 1, 0, // deletions + *path_buf, &path_len, 0); + op = BAM_CDEL; + op_len = target->data_len - (*path_buf) [0].i; + new_score = del_score; + pos_adj = op_len; + } + if ((*path_buf) [path_len - 1].ctype == TMAP_SW_FROM_I) + pos_adj++; // TODO: is this correct? + } + if(0 < new_score && 0 <= new_score - long_gap) // ?? Redundant condition (DK) + { + int32_t n_cigar_op = (0 < op_len) ? 1 : 0; + int32_t j; + // get the cigar + cigar = tmap_sw_path2cigar (*path_buf, path_len, &n_cigar); + if (0 == n_cigar) + tmap_bug (); + // re-allocate the cigar + dest_sam->cigar = tmap_realloc (dest_sam->cigar, sizeof (uint32_t) * (n_cigar_op + n_cigar + dest_sam->n_cigar), "dest_sam->cigar"); + // cigar_buf_reallocated = 1; + // shift up + for (j = dest_sam->n_cigar - 1; 0 <= j; j--) + dest_sam->cigar [j + n_cigar_op + n_cigar] = dest_sam->cigar [j]; + // add the operation + if (1 == n_cigar_op) // 0 < op_len + TMAP_SW_CIGAR_STORE (dest_sam->cigar [n_cigar], op, op_len); + // add the rest of the cigar + for(j = 0; j < n_cigar; j++) + dest_sam->cigar [j] = cigar [j]; + + uint32_t added_ref = 0, added_qry = 0; + dest_sam->n_cigar += n_cigar + n_cigar_op; + if (op == BAM_CDEL) + { + dest_sam->target_len += op_len; + added_ref += op_len; + } + else + added_qry += op_len; + + for (j = 0; j < n_cigar; j++) + { + int32_t oplen = TMAP_SW_CIGAR_LENGTH (cigar [j]); + switch (TMAP_SW_CIGAR_OP (cigar [j])) + { case BAM_CMATCH: + added_qry += oplen; + case BAM_CDEL: + added_ref += oplen; + dest_sam->target_len += oplen; + dest_sam->pos -= oplen; // NB: must adjust the position at the start of the alignment + break; case BAM_CINS: - case BAM_CSOFT_CLIP: - tttlen += TMAP_SW_CIGAR_LENGTH(s->cigar[j]); - break; - } - } - if (tttlen != seq_len) { - fprintf(stderr, "%d seqlen=%d tttlen=%d\t tail_skip=%d begin_clip=%d n_cigar=%d\n", path[path_len-1].ctype, seq_len, tttlen, tail_clip, begin_clip, s->n_cigar); - } - - // May need adjust start?? - target = tmp_target; - end++; i++; - // start = end; - // update aux data - tmap_map_sam_malloc_aux(s); - switch(s->algo_id) { - case TMAP_MAP_ALGO_MAP1: - (*s->aux.map1_aux) = (*tmp_sam.aux.map1_aux); - break; - case TMAP_MAP_ALGO_MAP2: - (*s->aux.map2_aux) = (*tmp_sam.aux.map2_aux); - break; - case TMAP_MAP_ALGO_MAP3: - (*s->aux.map3_aux) = (*tmp_sam.aux.map3_aux); - - case TMAP_MAP_ALGO_MAP4: - (*s->aux.map4_aux) = (*tmp_sam.aux.map4_aux); - break; - case TMAP_MAP_ALGO_MAPVSW: - (*s->aux.map_vsw_aux) = (*tmp_sam.aux.map_vsw_aux); - break; - default: - tmap_bug(); - break; - } - continue; - //} - } - } - /* - fprintf(stderr, "qlen=%d tlen=%d\n", qlen, tlen); - fprintf(stderr, "tmp_sam.result.query_start=%d\n", tmp_sam.result.query_start); - fprintf(stderr, "tmp_sam.result.query_end=%d\n", tmp_sam.result.query_end); - fprintf(stderr, "tmp_sam.result.target_start=%d\n", tmp_sam.result.target_start); - fprintf(stderr, "tmp_sam.result.target_end=%d\n", tmp_sam.result.target_end); - fprintf(stderr, "tmp_sam.result.n_best=%d\n", tmp_sam.result.n_best); - fprintf(stderr, "tmp_sam.pos=%u\n", tmp_sam.pos); - */ - - // NB: target_start and target_end are inverted for the reverse strand but we - // only care about their difference. - - // adjust the query and target based off of the start of the alignment - query = (uint8_t*)tmap_seq_get_bases(seqs[strand])->s; // forward genomic strand - tmp_target = target; - if(0 == strand) { - query_start = tmp_sam.result.query_start; - query_end = tmp_sam.result.query_end; - query += tmp_sam.result.query_start; // offset query - target += tmp_sam.result.target_start; - tmp_sam.pos += tmp_sam.result.target_start; // keep it zero based - } - else { - // <-------< QE <-------< QS <-------< - query_start = seq_len - tmp_sam.result.query_end - 1; - query_end = seq_len - tmp_sam.result.query_start - 1; - query += seq_len - tmp_sam.result.query_end - 1; // offset query - // does not affect target offset - // does not affect tmp_sam.pos; this was updated earlier - } - qlen = tmp_sam.result.query_end - tmp_sam.result.query_start + 1; // update query length - tlen = tmp_sam.result.target_end - tmp_sam.result.target_start + 1; - - /** - * Step 2: generate the cigar - */ - if(0 < conv) { // NB: there were IUPAC bases - // init iupac parameters, if necessary - if(0 == iupac_init) { - par_iupac.matrix = matrix_iupac; - __map_util_gen_ap_iupac(par_iupac, opt); - iupac_init = 1; - } - // update start/end - start_pos = tmp_sam.pos + 1; // one-based - end_pos = start_pos + tlen - 1; - target = tmp_target; // reset target in memory - if(target_mem < tlen) { // more memory? - target_mem = tlen; - tmap_roundup32(target_mem); - target = tmap_realloc(target, sizeof(uint8_t)*target_mem, "target"); - } - // Get the new target - // NB: IUPAC codes are turned into mismatches - if(NULL == tmap_refseq_subseq2(refseq, sams->sams[end].seqid+1, start_pos, end_pos, target, 0, NULL)) { - tmap_bug(); - } - tmp_target = target; // store for later - } - - // path memory - if(path_mem <= tlen + seq_len) { // lengthen the path - path_mem = tlen + seq_len; - tmap_roundup32(path_mem); - path = tmap_realloc(path, sizeof(tmap_sw_path_t)*path_mem, "path"); - } + case BAM_CSOFT_CLIP: + added_qry += oplen; + default: + break; + } + } + free (cigar); cigar = NULL; + // update the query end + int32_t orig_qry_start = dest_sam->result.query_start; + dest_sam->result.query_start = (*path_buf) [path_len - 1].j - 1; // query_start is one-based | DK: this should read path.j is one-based + if (query_start < 0) + tmap_bug(); + dest_sam->score += new_score - long_gap; + dest_sam->pos -= pos_adj; // adjust the position further if there was a deletion + // merge adjacent cigar operations + tmap_map_util_merge_adjacent_cigar_operations (dest_sam); + + // update alignment box + // query_end does not change + assert (dest_sam->result.query_start + added_qry == orig_qry_start); + dest_sam->result.target_end += added_ref; + uint32_t read_side = (dest_sam->strand==0)?F5P:R3P; + stat->num_salvaged [read_side] ++; + stat->bases_salvaged_qry [read_side] += added_qry; + stat->bases_salvaged_ref [read_side] += added_ref; + stat->score_salvaged_total [read_side] += new_score - long_gap; + salvaged = 1; + } + } + break; + } - /* - // Debugging - for(j=0;jgapl_len; + start_pos = dest_sam->pos + dest_sam->target_len + 1; + end_pos = start_pos + tglen - 1; + if (refseq->annos [dest_sam->seqid].len < end_pos) // bound + end_pos = refseq->annos [dest_sam->seqid].len; // one-based + + if (end_pos < start_pos) + break; // exit the loop + int32_t long_gap = opt->pen_gapl; + + //ZZ: check amplicon + uint32_t ampl_start = 0, ampl_end = 0; + if (ampl_exist || tmap_map_get_amplicon (refseq, dest_sam->seqid, dest_sam->pos, dest_sam->pos + dest_sam->target_len, &l_start, &l_end, dest_sam->strand)) + { + ampl_exist = 1; + if (ampl_end <= dest_sam->pos + dest_sam->target_len + 1) + break; + if (end_pos < ampl_end) + end_pos = ampl_end; + long_gap = 0; + } - s = &sams_tmp->sams[i]; - - // shallow copy previous data - (*s) = tmp_sam; - - // Smith Waterman with banding - // NB: we store the score from the banded version, which does not allow - // ins then del, or del then ins. The vectorized version does. - // NB: left genomic indel justification is facilitated by always using the - // forward strand target/query combination. - // NB: iupac bases may also increase the score - if(0 < conv) { // NB: there were IUPAC bases - s->score = tmap_sw_global_banded_core(target, tlen, query, qlen, &par_iupac, - tmp_sam.result.score_fwd, path, &path_len, 0); - } - else { - s->score = tmap_sw_global_banded_core(target, tlen, query, qlen, &par, - tmp_sam.result.score_fwd, path, &path_len, 0); - } + cache_target (target, refseq, dest_sam->seqid + 1, start_pos, end_pos); - // ZZ: There can be case when the global score is the same as the vectorized version - // whileas the alignments are different. We are not addressing all of them, but the - // cases where the resulting alignment starts or ends with deletion shall be avoided. - if( (s->score != tmp_sam.score && 1 < tmp_sam.result.n_best) - || (path_len > 0 && (TMAP_SW_FROM_D == path[0].ctype || TMAP_SW_FROM_D == path[path_len-1].ctype))) - { - /* - fprintf(stderr, "s->score=%d tmp_sam.score=%d\n", s->score, tmp_sam.score); - fprintf(stderr, "tmp_sam.result.n_best=%d\n", tmp_sam.result.n_best); - */ + // try to add a larger deletion + // DK: BUG (in original code): this works only if the prefix salvage procedure above did not succeed. Otherwise, the query points to the new start of aligned region, + // so that query + seq_len points beyond the end of valid sequence (and possibly beyond the end of the buffer) - // TS-9081 (DVK): catch the state of the input parameters -#ifdef CHECK_SWCORE_PARAMS - // qlen and query - int32_t saved_query_len = qlen; - uint8_t* saved_query = alloca (qlen+1); - memcpy (saved_query, query, qlen); saved_query [qlen] = 0; - // tlen and target - int32_t saved_target_len = tlen; - unit8_t* saved_target = alloca (tlen+1); - memcpy (saved_target, target, tlen); saved_target [tlen] = 0; - // SW parameters - tmap_sw_param_t saved_params; - memcpy (&saved_params, (0 < conv)? &par_iupac : &par, sizeof (tmap_sw_param_t)); - saved_params.matrix = alloca (saved_params.row * saved_params.row * sizeof (int32_t)); - memcpy (saved_params.matrix, (0 < conv)? par_iupac.matrix : par.matrix, saved_params.row * saved_params.row * sizeof (int32_t)); -#endif - - // explicitly fit the query into the target - if(0 < conv) { // NB: there were IUPAC bases - s->score = tmap_sw_fitting_core(target, tlen, query, qlen, &par_iupac, path, &path_len, 0); - } - else { - s->score = tmap_sw_fitting_core(target, tlen, query, qlen, &par, path, &path_len, 0); - } + del_score = tmap_sw_clipping_core2 (target->data, target->data_len, orig_query + query_end + 1, orig_query_len - query_end - 1, + par, + 1, 1, // allow the start and end of the target to be skipped + 0, 1, // deletions + NULL, 0, 0); -#ifdef CHECK_SWCORE_PARAMS - tmap_sw_param_t* pp = (0 < conv)? &par_iupac : ∥ - if (qlen != saved_query_len || - tlen != saved_target_len || - memcmp (saved_query_len, query, qlen) || - memcmp (saved_target_len, target, tlen) || - pp->gap_open != saved_params.gap_open || - pp->gap_ext != saved_params.gap_ext || - pp->gap_end != saved_params.gap_end || - pp->row != saved_params.row || - pp->band_width != saved_params.gap_width || - memcmp (pp->matrix, saved_params.matrix, saved_params.row * saved_params.row * sizeof (int32_t))) - { - fprintf (stderr, "\nUpper stack data changed during the function call:\n"); - fprintf (stderr, "Read: %s (%ld bp)\n", qry_name, qry_seq->l); - fprintf (stderr, "Full Query (%4ld bp): %s\n", qry_seq->l, qry_seq->s); - fprintf (stderr, "Query (%4d bp): %s\n", qlen, query); - fprintf (stderr, "Subject (%4d bp): ", tlen); - for (iii = 0; iii != tlen; ++iii) - fputc ("ACGTN" [(target [iii] < 4) ? target [iii] : 4], stderr); - fputc ('\n', stderr); - fprintf (stderr, "score is %d; tmp_sam.score is %d\n", s->score, tmp_sam.score); - tmap_bug (); - } -#endif + // try to add a larger insertion + ins_score = tmap_sw_clipping_core2 (target->data, target->data_len, orig_query + query_end + 1, orig_query_len - query_end - 1, + par, + 0, 1, // only allow the end of the target to be skipped + 1, 1, // insertions, + NULL, 0, 0); + + if(del_score <= 0 && ins_score <= 0) + // do nothing + new_score = -1; + else + { + // path memory + if (*path_buf_sz <= target->data_len + orig_query_len - query_end - 1) + { // lengthen the path + *path_buf_sz = target->data_len + orig_query_len - query_end - 1; + tmap_roundup32 (*path_buf_sz); + *path_buf = tmap_realloc (*path_buf, sizeof (tmap_sw_path_t) * *path_buf_sz, "path_buf"); + } + if (del_score < ins_score) + { + // get the path + tmap_sw_clipping_core2 (target->data, target->data_len, orig_query + query_end + 1, orig_query_len - query_end - 1, + par, + 0, 1, // only allow the end of the target to be skipped + 1, 1, // insertions, + *path_buf, &path_len, 0); + op = BAM_CINS; + op_len = (*path_buf) [path_len - 1].j - 1; + new_score = ins_score; + } + else + { + // get the path + tmap_sw_clipping_core2 (target->data, target->data_len, orig_query + query_end + 1, orig_query_len - query_end - 1, + par, + 1, 1, // allow the start and end of the target to be skipped + 0, 1, // deletions + *path_buf, &path_len, 0); + op = BAM_CDEL; + op_len = (*path_buf) [path_len - 1].i - 1; + new_score = del_score; + } + } + if(0 < new_score && 0 <= new_score - long_gap) //?? redundant condition (DK) + { + int32_t n_cigar_op = (0 < op_len) ? 1 : 0; + int32_t j; + // get the cigar + cigar = tmap_sw_path2cigar (*path_buf, path_len, &n_cigar); + if (0 == n_cigar) + tmap_bug (); + // re-allocate the cigar + dest_sam->cigar = tmap_realloc (dest_sam->cigar, sizeof (uint32_t) * (n_cigar_op + n_cigar + dest_sam->n_cigar), "dest_sam->cigar"); + // cigar_buf_reallocated = 1; + // add the operation + uint32_t added_ref = 0, added_qry = 0; + if (1 == n_cigar_op) // 0 < op_len + { + TMAP_SW_CIGAR_STORE (dest_sam->cigar [dest_sam->n_cigar], op, op_len); + if (op == BAM_CDEL) + { + dest_sam->target_len += op_len; + added_ref += op_len; + } + else + added_qry += op_len; - int32_t leading, trailing; - while(1) { - // check to see if there are leading/trailing deletions, then redo - leading = trailing = 0; - // leading - for(j=path_len-1;0<=j;j--) { - if(TMAP_SW_FROM_D == path[j].ctype) leading++; - else break; - } - // trailing - for(j=0;js; - tmap_string_t* qry_seq = tmap_seq_get_bases (seq); - - fprintf (stderr, "\nLead/trail delete on read: %s (%ld bp), leading = %d, trailing = %d\n", qry_name, qry_seq->l, leading, trailing); - fprintf (stderr, "Full Query (%4ld bp): %s\n", qry_seq->l, qry_seq->s); - fprintf (stderr, "Query (%4d bp): %s\n", qlen, query); - // target in alignment - fprintf (stderr, "Subject (%4d bp): ", tlen); - for (iii = 0; iii != tlen; ++iii) - fputc ("ACGTN" [(target [iii] < 4) ? target [iii] : 4], stderr); - fputc ('\n', stderr); - fprintf (stderr, "score is %d; tmp_sam.score is %d\n", s->score, tmp_sam.score); - fprintf (stderr, "Alignment path (%d positions):\n", path_len); - for (iii = 0; iii != path_len; ++iii) - fprintf (stderr, "%3d", path [iii].i); - fputc ('\n', stderr); - for (iii = 0; iii != path_len; ++iii) - fprintf (stderr, "%3c", "MIDSN" [(path [iii].ctype < 4) ? path [iii].ctype : 4]); - fputc ('\n', stderr); - for (iii = 0; iii != path_len; ++iii) - fprintf (stderr, "%3d", path [iii].j); - fputc ('\n', stderr); - fprintf (stderr, "Reference Id: %d, strand: %d, position: %d", sams->sams[end].seqid, sams->sams[end].strand, sams->sams[end].pos); - - fprintf (stderr, "n_best: %d\n", tmp_sam.result.n_best); - fprintf (stderr, "Converted IUPAC bases in reference: %d\n", conv); - - tmap_sw_param_t *pp = (0 == conv)? &par : &par_iupac; - fprintf (stderr, "SW parameters:\n"); - fprintf (stderr, " band width = %d\n", pp->band_width); /*!< for Smith-Waterman banding */ - fprintf (stderr, " gap open = %d\n", pp->gap_open); /*!< gap open penalty (positive) */ - fprintf (stderr, " gap_ext = %d\n", pp->gap_ext); /*!< gap extension penalty (positive) */ - fprintf (stderr, " gap_end = %d\n", pp->gap_end); /*!< gap end penalty (positive */ - fprintf (stderr, " alphabet size = %d\n", pp->row); - unsigned rr, cc; - for (rr = 0; rr != pp->row; ++rr) - { - fprintf (stderr, " %3u ", rr); - for (cc = 0; cc != pp->row; ++cc) - { - fprintf (stderr, "%5d", pp->matrix [pp->row*rr + cc]); - } - fprintf (stderr, "\n"); - } - } - - tmap_bug(); // NB: this should not happen with tmap_sw_fitting_core - } - // TODO: should we skip otherwise? No, since VSW can differfrom GSW - } + } + // add the rest of the cigar + for (j = 0; j < n_cigar; j++) + dest_sam->cigar [j + dest_sam->n_cigar + n_cigar_op] = cigar [j]; + + dest_sam->n_cigar += n_cigar + n_cigar_op; + for (j = 0; j < n_cigar; j++) + { + int32_t oplen = TMAP_SW_CIGAR_LENGTH (cigar [j]); + switch (TMAP_SW_CIGAR_OP (cigar [j])) + { + case BAM_CMATCH: + added_qry += oplen; + case BAM_CDEL: + added_ref += oplen; + dest_sam->target_len += oplen; + break; + case BAM_CINS: + case BAM_CSOFT_CLIP: + added_qry += oplen; + default: + break; + } + } + free (cigar); cigar = NULL; + // update the query end + query_end += (*path_buf) [0].j; // query_end is zero-based + if (orig_query_len <= query_end) + tmap_bug(); + dest_sam->score += new_score - long_gap; + // merge adjacent cigar operations + tmap_map_util_merge_adjacent_cigar_operations (dest_sam); + // update alignment box + + dest_sam->result.query_end += added_qry; + dest_sam->result.target_end += added_ref; + if (dest_sam->result.query_end != query_end) + tmap_bug (); + if (dest_sam->result.target_end != dest_sam->target_len - 1) + tmap_bug (); + + uint32_t read_side = (dest_sam->strand==0)?F3P:R5P; + stat->num_salvaged [read_side] ++; + stat->bases_salvaged_qry [read_side] += added_qry; + stat->bases_salvaged_ref [read_side] += added_ref; + stat->score_salvaged_total [read_side] += new_score - long_gap; + salvaged = 1; } + } + break; + } + extend_softclips_to_read_edges (dest_sam, seqs); + stat->reads_salvaged += salvaged; +} - s->pos = s->pos + (path[path_len-1].i-1); // zero-based - if(path[path_len-1].ctype == TMAP_SW_FROM_I) { // don't quite understand this ZZ - s->pos++; - } - s->cigar = tmap_sw_path2cigar(path, path_len, &s->n_cigar); - if(0 == s->n_cigar) { - tmap_bug(); - } - s->target_len = 0; - for(j=0;jn_cigar;j++) { - switch(TMAP_SW_CIGAR_OP(s->cigar[j])) { +static void update_align_box_and_tlen (tmap_map_sam_t* dest_sam) +{ + // assumes pos and cigar are correct + // update box from cigar + dest_sam->result.target_start = dest_sam->result.target_end = 0; + dest_sam->result.query_start = dest_sam->result.query_end = 0; + uint32_t *cigar_el_p, *cent; + for (cigar_el_p = dest_sam->cigar, cent = dest_sam->cigar + dest_sam->n_cigar; cigar_el_p != cent; ++ cigar_el_p ) + { + uint32_t op = TMAP_SW_CIGAR_OP (*cigar_el_p); + uint32_t oplen = TMAP_SW_CIGAR_LENGTH (*cigar_el_p); + switch (op) + { case BAM_CMATCH: + dest_sam->result.query_end += oplen; + dest_sam->result.target_end += oplen; + break; case BAM_CDEL: - s->target_len += TMAP_SW_CIGAR_LENGTH(s->cigar[j]); - break; + dest_sam->result.target_end += oplen; + break; + case BAM_CINS: + dest_sam->result.query_end += oplen; + break; + case BAM_CSOFT_CLIP: + if (cigar_el_p == dest_sam->cigar) // very first operation + dest_sam->result.query_start = dest_sam->result.query_end = oplen; + break; default: - break; - } - } + tmap_bug (); + } + } + dest_sam->target_len = dest_sam->result.target_end; + // adjust for inclusivity + if (dest_sam->result.query_end != dest_sam->result.query_start) + --dest_sam->result.query_end; + if (dest_sam->result.target_end != dest_sam->result.target_start) + --dest_sam->result.target_end; +} - /** - * Try to detect large indels present as soft-clipped prefixes of the - * alignment. - */ - uint32_t ampl_exist = 0; - while(1) { // NB: so we can break out at any time - if(0 < opt->pen_gapl && 0 < query_start) { // start of the alignment - // ZZ:We may check amplicon here as well. May try this only when it is near start of an amplicon. - // Here the reference mapping position is known by s->pos to s->pos+s->target_len - // We can check the amplicon with these. - // A. When bed file is not present, current logic holds. - // B.i. If bed file is given, when an amplicon is present, try use all the bases to the begin/end of amplicon, very small or no long gap penalty. - // B.ii.If bed file is given and the hit position is not in an amplicon, normal logic apply. - - int32_t del_score = 0; - int32_t ins_score = 0; - uint32_t op, op_len; - int32_t new_score; - uint32_t *cigar = NULL; - int32_t n_cigar; - int32_t pos_adj = 0; - - query = (uint8_t*)tmap_seq_get_bases(seqs[strand])->s; // forward genomic strand - - // get the target sequence before the start of the alignment - tlen = query_start + opt->gapl_len; - if(s->pos <= tlen) start_pos = 1; - else start_pos = s->pos - tlen + 1; - end_pos = s->pos; - if(end_pos < start_pos) break; // exit the loop - tlen = end_pos - start_pos + 1; - int32_t long_gap = opt->pen_gapl; - //ZZ: check amplicon - if (tmap_map_get_amplicon(refseq, s->seqid, s->pos, s->pos+s->target_len, &l_start, &l_end, strand)) { - ampl_exist = 1; - if (ampl_start >= s->pos) break; - if (start_pos > ampl_start) { - start_pos = ampl_start; - tlen = end_pos - start_pos + 1; - } - //Alternative, align only to the begin of the amplicon. - /* - start_pos = ampl_start; - tlen = end_pos - start_pos + 1; - */ - long_gap = 0; - } +void key_trim_alignment ( + tmap_map_sam_t* dest_sam, // mapping being trimmed + tmap_seq_t** seqs, // array of size 4 that contains pre-computed inverse / complement combinations + tmap_seq_t* seq, // read + tmap_refseq_t* refseq, // reference server + ref_buf_t* target, // reference data + tmap_map_stats_t *stat // statistics +) +{ - // target mem - target = tmp_target; // reset target in memory - if(target_mem < tlen) { // more memory? - target_mem = tlen; - tmap_roundup32(target_mem); - target = tmap_realloc(target, sizeof(uint8_t)*target_mem, "target"); - tmp_target = target; // store for later - } - // Get the new target - // NB: IUPAC codes are turned into mismatches - if(NULL == tmap_refseq_subseq2(refseq, s->seqid+1, start_pos, end_pos, target, 0, &conv)) { - tmap_bug(); - } - if(0 < conv && 0 == iupac_init) { - par_iupac.matrix = matrix_iupac; - __map_util_gen_ap_iupac(par_iupac, opt); - iupac_init = 1; - } + // get first base + uint8_t key_base = INT8_MAX; + if (seq->ks) + key_base = tmap_nt_char_to_int [(uint8_t) seq->ks [strlen (seq->ks) - 1]]; // NB: last base of the key + // get the new target + // cache data + if (INT8_MAX != key_base) + { + int32_t qlen = dest_sam->result.query_end - dest_sam->result.query_start; // update query length + uint8_t* orig_query = (uint8_t*) tmap_seq_get_bases (seqs [dest_sam->strand])->s; // forward genomic strand + uint8_t* adjusted_query = orig_query + dest_sam->result.query_start; + uint32_t start_pos = dest_sam->pos + 1; + uint32_t end_pos = start_pos + dest_sam->target_len - 1; + cache_target (target, refseq, dest_sam->seqid + 1, start_pos, end_pos); + tmap_map_util_keytrim (adjusted_query, qlen, target->data, target->data_len, dest_sam->strand, key_base, dest_sam, stat); + // only pos, cigar and n_cigar are updated by tmap_map_util_keytrim + // for end-repair, we need target_len and the alignment box also updated + // update them here + update_align_box_and_tlen (dest_sam); + } +} - // try to add a larger deletion - del_score = tmap_sw_clipping_core2(target, tlen, query, query_start, - (0 < conv) ? &par_iupac : &par, - 1, 1, // allow the start and end of the target to be skipped - 1, 0, // deletions - NULL, 0, 0); - - // try to add a larger insertion - // TODO: how do we enforce that the target starts immediately where - // we want it to? - ins_score = tmap_sw_clipping_core2(target, tlen, query, query_start, - (0 < conv) ? &par_iupac : &par, - 1, 0, // only allow the start of the target to be skipped - 1, 1, // insertions, - NULL, 0, 0); - // reset start/end position - start_pos = s->pos + 1; - end_pos = s->pos + s->target_len; - - if(del_score <= 0 && ins_score <= 0) { - // do nothing - new_score = -1; - } - else { - if(del_score < ins_score) { - // get the path - tmap_sw_clipping_core2(target, tlen, query, query_start, - (0 < conv) ? &par_iupac : &par, - 1, 0, // only allow the start of the target to be skipped - 1, 1, // insertions, - path, &path_len, 0); - op = BAM_CINS; - op_len = query_start - path[0].j; - new_score = ins_score; - pos_adj = 0; - } - else { - // get the path - tmap_sw_clipping_core2(target, tlen, query, query_start, - (0 < conv) ? &par_iupac : &par, - 1, 1, // allow the start and end of the target to be skipped - 1, 0, // deletions - path, &path_len, 0); - op = BAM_CDEL; - op_len = tlen - path[0].i; - new_score = del_score; - pos_adj = op_len; - } - if(path[path_len-1].ctype == TMAP_SW_FROM_I) pos_adj++; // TODO: is this correct? - } - if(0 < new_score && 0 <= new_score - long_gap) { - int32_t n_cigar_op = (0 < op_len) ? 1 : 0; - // get the cigar - cigar = tmap_sw_path2cigar(path, path_len, &n_cigar); - if(0 == n_cigar) { - tmap_bug(); - } - // re-allocate the cigar - s->cigar = tmap_realloc(s->cigar, sizeof(uint32_t)*(n_cigar_op+n_cigar+s->n_cigar), "s->cigar"); - // shift up - for(j=s->n_cigar-1;0<=j;j--) { - s->cigar[j+n_cigar_op+n_cigar] = s->cigar[j]; - } - // add the operation - if(1 == n_cigar_op) { // 0 < op_len - TMAP_SW_CIGAR_STORE(s->cigar[n_cigar], op, op_len); - } - // add the rest of the cigar - for(j=0;jcigar[j] = cigar[j]; - } - s->n_cigar += n_cigar + n_cigar_op; - for(j=0;jtarget_len += TMAP_SW_CIGAR_LENGTH(cigar[j]); - s->pos -= TMAP_SW_CIGAR_LENGTH(cigar[j]); // NB: must adjust the position at the start of the alignment - break; - default: - break; - } - } - free(cigar); cigar = NULL; - // update the query end - query_start = path[path_len-1].j - 1; // query_start is one-based - if(query_start < 0) tmap_bug(); - s->score += new_score - long_gap; - s->pos -= pos_adj; // adjust the position further if there was a deletion - // merge adjacent cigar operations - tmap_map_util_merge_adjacent_cigar_operations(s); - } +// The 3' end-repair may alter the alignment and then 5' end-repair should get buffers adjusted accordingly. +// The IR-28816 reveals condition where both 3' and 5' are repairable, and 5' repair used unajusted positions, causing buffer overrun. +// This function serves as a wrapper arounf both 3' and 5' end-repair, correcting SCs and adjusting coordinates and buffers independently. +// returns code for stats collection: 0 for no adjustment, 1 for softclip, 2 for extension +// note: A bit redundant function - it could do a little less if softclip to insert conversion is done only for one proper end of the read. +// Not too much extra work though, and the logic is slightly cleaner. + +static int32_t end_repair_helper ( + tmap_map_sam_t* dest_sam, // mapping being trimmed + tmap_seq_t** seqs, // array of size 4 that contains pre-computed inverse / complement combinations + tmap_seq_t* seq, // read + tmap_refseq_t* refseq, // reference server + ref_buf_t* target, // reference data + tmap_sw_path_t** path_buf, // buffer for traceback path + int32_t* path_buf_sz, // used portion and allocated size of traceback path. + int32_t softclip_start, // softclip allowed at read 5' + int32_t softclip_end, // softclip allowed at read 3' + tmap_map_opt_t* opt, // tmap options + tmap_map_stats_t *stat, // statistics + int32_t five_prime // 1 to repair 5', 0 to repairt 3' +) +{ + uint8_t* orig_query = (uint8_t*) tmap_seq_get_bases (seqs [dest_sam->strand])->s; // forward genomic strand + int32_t orig_qlen = tmap_seq_get_bases_length (seqs [0]); + int32_t qlen = dest_sam->result.query_end - dest_sam->result.query_start + 1; // update query length + uint8_t* adjusted_query = orig_query + dest_sam->result.query_start; + + // tmap_map_util_end_repair expects no softclips if they are not explicitely allowed; + // if there are such, replace them with INSs + if (TMAP_SW_CIGAR_OP (dest_sam->cigar [0]) == BAM_CSOFT_CLIP && + ((softclip_start == 0 && dest_sam->strand == 0) || + (softclip_end == 0 && dest_sam->strand == 1))) + { + int32_t op_len = TMAP_SW_CIGAR_LENGTH (dest_sam->cigar [0]); + TMAP_SW_CIGAR_STORE (dest_sam->cigar [0], BAM_CINS, op_len); + qlen += op_len; + adjusted_query -= op_len; + } + if (TMAP_SW_CIGAR_OP (dest_sam->cigar [dest_sam->n_cigar-1]) == BAM_CSOFT_CLIP && + ((softclip_end == 0 && dest_sam->strand == 0) || + (softclip_start == 0 && dest_sam->strand == 1))) + { + int32_t op_len = TMAP_SW_CIGAR_LENGTH (dest_sam->cigar [dest_sam->n_cigar - 1]); + TMAP_SW_CIGAR_STORE (dest_sam->cigar [dest_sam->n_cigar - 1], BAM_CINS, op_len); + qlen += op_len; + } - // reset start/end position - start_pos = s->pos + 1; - end_pos = s->pos + s->target_len; - if(end_pos < start_pos) tmap_bug(); - - // retrieve the target - //TODO: is this always necessary (keytrim)? - // target mem - tlen = end_pos - start_pos + 1; - if(tlen <= 0) tmap_bug(); - target = tmp_target; // reset target in memory - if(target_mem < tlen) { // more memory? - target_mem = tlen; - tmap_roundup32(target_mem); - target = tmap_realloc(target, sizeof(uint8_t)*target_mem, "target"); - tmp_target = target; // store for later - } - // Get the new target - // NB: IUPAC codes are turned into mismatches - if(NULL == tmap_refseq_subseq2(refseq, s->seqid+1, start_pos, end_pos, target, 0, &conv)) { - tmap_bug(); - } - if(0 < conv && 0 == iupac_init) { - par_iupac.matrix = matrix_iupac; - __map_util_gen_ap_iupac(par_iupac, opt); - iupac_init = 1; - } + // extend cached reference enough to support convertion of softclips to matches (tmap_map_util_end_repair assumes they are in) + uint32_t sc5 = 0, sc3 = 0; + if (dest_sam->n_cigar && TMAP_SW_CIGAR_OP (dest_sam->cigar [0]) == BAM_CSOFT_CLIP) + sc5 = TMAP_SW_CIGAR_LENGTH (dest_sam->cigar [0]); + if (dest_sam->n_cigar > 1 && TMAP_SW_CIGAR_OP (dest_sam->cigar [dest_sam->n_cigar - 1]) == BAM_CSOFT_CLIP) + sc3 = TMAP_SW_CIGAR_LENGTH (dest_sam->cigar [dest_sam->n_cigar - 1]); + + // the cigar is in the query direction, convert 5' - 3' to target coords + if (dest_sam->strand == 1) + { + uint32_t t = sc5; + sc5 = sc3; + sc3 = t; + } + // do not go below 0 or over contig length + uint32_t start_pos, end_pos; + if (dest_sam->pos + dest_sam->result.target_start < sc5) + start_pos = 1; + else + start_pos = dest_sam->pos + dest_sam->result.target_start - sc5 + 1; + end_pos = dest_sam->pos + dest_sam->result.target_end + sc3 + 1; + if (end_pos > refseq->annos [dest_sam->seqid].len) + end_pos = refseq->annos [dest_sam->seqid].len; + // cache the data + cache_target (target, refseq, dest_sam->seqid + 1, start_pos, end_pos); + + // massage target cache internals. This would not affect integrity of cache + uint8_t* orig_data = target->data; + target->data = orig_data + (dest_sam->pos + 1 - start_pos); + target->data_len = dest_sam->target_len; + + int32_t result = tmap_map_util_end_repair (seq, adjusted_query, qlen, target->data, target->data_len, dest_sam->strand, path_buf, path_buf_sz, refseq, dest_sam, opt, five_prime, stat); + + assert (dest_sam->n_cigar); + dest_sam->result.query_start = (TMAP_SW_CIGAR_OP (dest_sam->cigar [0]) == BAM_CSOFT_CLIP) ? (TMAP_SW_CIGAR_LENGTH (dest_sam->cigar [0])) : 0; + dest_sam->result.query_end = (TMAP_SW_CIGAR_OP (dest_sam->cigar [dest_sam->n_cigar - 1]) == BAM_CSOFT_CLIP) ? (orig_qlen - TMAP_SW_CIGAR_LENGTH (dest_sam->cigar [dest_sam->n_cigar - 1]) - 1) : orig_qlen - 1; + dest_sam->result.target_start = 0; + dest_sam->result.target_end = dest_sam->target_len - 1; + + return result; +} - // reset query - query = (uint8_t*)tmap_seq_get_bases(seqs[strand])->s; // forward genomic strand - query += query_start; - } - break; - } - - /** - * Try to detect large indels present as soft-clipped suffixes of the - * alignment. - */ - while(1) { // NB: so we can break out at any time - if(0 < opt->pen_gapl && query_end < seq_len-1) { - int32_t del_score = 0; - int32_t ins_score = 0; - uint32_t op, op_len; - int32_t new_score = 0; - uint32_t *cigar = NULL; - int32_t n_cigar; - - // get the target sequence after the end of the alignment. - tlen = seq_len - 1 - query_end + opt->gapl_len; - start_pos = s->pos + s->target_len + 1; - end_pos = start_pos + tlen - 1; - if(refseq->annos[s->seqid].len < end_pos) { // bound - end_pos = refseq->annos[s->seqid].len; // one-based - } - if(end_pos < start_pos) break; // exit the loop - tlen = end_pos - start_pos + 1; - int32_t long_gap = opt->pen_gapl; - - //ZZ: check amplicon - if (ampl_exist || tmap_map_get_amplicon(refseq, s->seqid, s->pos, s->pos+s->target_len, &l_start, &l_end, strand)) { - ampl_exist = 1; - if (ampl_end <= s->pos+s->target_len + 1) break; - if (end_pos < ampl_end) { - end_pos = ampl_end; - tlen = end_pos - start_pos + 1; - } - //Alternative, align only to the end of the amplicon. - /* - end_pos = ampl_end; - tlen = end_pos - start_pos + 1; - */ - long_gap = 0; - } +void end_repair ( + tmap_map_sam_t* dest_sam, // mapping being trimmed + tmap_seq_t** seqs, // array of size 4 that contains pre-computed inverse / complement combinations + tmap_seq_t* seq, // read + tmap_refseq_t* refseq, // reference server + ref_buf_t* target, // reference data + tmap_sw_path_t** path_buf, // buffer for traceback path + int32_t* path_buf_sz, // used portion and allocated size of traceback path. + tmap_map_opt_t* opt, // tmap options + tmap_map_stats_t *stat // statistics +) +{ + int32_t softclip_start, softclip_end; + tmap_map_util_set_softclip (opt, seq, &softclip_start, &softclip_end); - // target mem - target = tmp_target; // reset target in memory - if(target_mem < tlen) { // more memory? - target_mem = tlen; - tmap_roundup32(target_mem); - target = tmap_realloc(target, sizeof(uint8_t)*target_mem, "target"); - tmp_target = target; // store for later - } - // Get the new target - // NB: IUPAC codes are turned into mismatches - if(NULL == tmap_refseq_subseq2(refseq, s->seqid+1, start_pos, end_pos, target, 0, &conv)) { - tmap_bug(); - } - if(0 < conv && 0 == iupac_init) { - par_iupac.matrix = matrix_iupac; - __map_util_gen_ap_iupac(par_iupac, opt); - iupac_init = 1; - } + int32_t r3 = end_repair_helper (dest_sam, seqs, seq, refseq, target, path_buf, path_buf_sz, softclip_start, softclip_end, opt, stat, 0); - // try to add a larger deletion - del_score = tmap_sw_clipping_core2(target, tlen, query + query_end + 1, seq_len - query_end - 1, - (0 < conv) ? &par_iupac : &par, - 1, 1, // allow the start and end of the target to be skipped - 0, 1, // deletions - NULL, 0, 0); - - // try to add a larger insertion - ins_score = tmap_sw_clipping_core2(target, tlen, query + query_end + 1, seq_len - query_end - 1, - (0 < conv) ? &par_iupac : &par, - 0, 1, // only allow the end of the target to be skipped - 1, 1, // insertions, - NULL, 0, 0); - - if(del_score <= 0 && ins_score <= 0) { - // do nothing - new_score = -1; - } - else { - if(del_score < ins_score) { - // get the path - tmap_sw_clipping_core2(target, tlen, query + query_end + 1, seq_len - query_end - 1, - (0 < conv) ? &par_iupac : &par, - 0, 1, // only allow the end of the target to be skipped - 1, 1, // insertions, - path, &path_len, 0); - op = BAM_CINS; - op_len = path[path_len-1].j - 1; - new_score = ins_score; - } - else { - // get the path - tmap_sw_clipping_core2(target, tlen, query + query_end + 1, seq_len - query_end - 1, - (0 < conv) ? &par_iupac : &par, - 1, 1, // allow the start and end of the target to be skipped - 0, 1, // deletions - path, &path_len, 0); - op = BAM_CDEL; - op_len = path[path_len-1].i - 1; - new_score = del_score; - } - } - if(0 < new_score && 0 <= new_score - long_gap) { - int32_t n_cigar_op = (0 < op_len) ? 1 : 0; - // get the cigar - cigar = tmap_sw_path2cigar(path, path_len, &n_cigar); - if(0 == n_cigar) { - tmap_bug(); - } - // re-allocate the cigar - s->cigar = tmap_realloc(s->cigar, sizeof(uint32_t)*(n_cigar_op+n_cigar+s->n_cigar), "s->cigar"); - // add the operation - if(1 == n_cigar_op) { // 0 < op_len - TMAP_SW_CIGAR_STORE(s->cigar[s->n_cigar], op, op_len); - } - // add the rest of the cigar - for(j=0;jcigar[j+s->n_cigar+n_cigar_op] = cigar[j]; - } - s->n_cigar += n_cigar + n_cigar_op; - for(j=0;jtarget_len += TMAP_SW_CIGAR_LENGTH(cigar[j]); - break; - default: - break; - } - } - free(cigar); cigar = NULL; - // update the query end - query_end += path[0].j; // query_end is zero-based - if(seq_len <= query_end) tmap_bug(); - s->score += new_score - long_gap; - // merge adjacent cigar operations - tmap_map_util_merge_adjacent_cigar_operations(s); - } + int32_t r5 = end_repair_helper (dest_sam, seqs, seq, refseq, target, path_buf, path_buf_sz, softclip_start, softclip_end, opt, stat, 1); - // reset start/end position - start_pos = s->pos + 1; - end_pos = s->pos + s->target_len; - - // retrieve the target - //TODO: is this always necessary (keytrim)? - // target mem - tlen = end_pos - start_pos + 1; - target = tmp_target; // reset target in memory - if(target_mem < tlen) { // more memory? - target_mem = tlen; - tmap_roundup32(target_mem); - target = tmap_realloc(target, sizeof(uint8_t)*target_mem, "target"); - tmp_target = target; // store for later - } - // Get the new target - // NB: IUPAC codes are turned into mismatches - if(NULL == tmap_refseq_subseq2(refseq, s->seqid+1, start_pos, end_pos, target, 0, &conv)) { - tmap_bug(); - } - if(0 < conv && 0 == iupac_init) { - par_iupac.matrix = matrix_iupac; - __map_util_gen_ap_iupac(par_iupac, opt); - iupac_init = 1; - } - } - break; - } + // update alignment box (dest_sam->result), as it may be used downstream + // assume alignment is correct here - covers entire read and softclips are properly extended + if (r3 != 0 || r5 != 0) + stat->reads_end_repair_clipped++; + if (r3 == 2 || r5 == 2) + stat->reads_end_repair_extended++; +} - // add soft clipping - if(0 < query_start) { - // soft clip the front of the read - s->cigar = tmap_realloc(s->cigar, sizeof(uint32_t)*(1+s->n_cigar), "s->cigar"); - for(j=s->n_cigar-1;0<=j;j--) { // shift up - s->cigar[j+1] = s->cigar[j]; - } - TMAP_SW_CIGAR_STORE(s->cigar[0], BAM_CSOFT_CLIP, query_start); - s->n_cigar++; - } - if(query_end < seq_len-1) { - // soft clip the end of the read - s->cigar = tmap_realloc(s->cigar, sizeof(uint32_t)*(1+s->n_cigar), "s->cigar"); - TMAP_SW_CIGAR_STORE(s->cigar[s->n_cigar], BAM_CSOFT_CLIP, seq_len - query_end - 1); - s->n_cigar++; - } +tmap_map_sams_t* +tmap_map_util_find_align_starts ( + tmap_refseq_t *refseq, // reference server + tmap_map_sams_t *sams, // initial rough mapping + tmap_seq_t *seq, // read + tmap_seq_t **seqs, // array of size 4 that contains pre-computed inverse / complement combinations + tmap_map_opt_t *opt, // tmap parameters + ref_buf_t* target, // reference data + tmap_map_stats_t* stat // statistics + +) +{ + int32_t end; // index for the alignment being evaluated + int32_t i; // index into results buffer; + + tmap_map_sams_t *sams_tmp = NULL; // buffer to receive the alignments + int32_t orig_query_len = 0; // current read size + + tmap_vsw_t *vsw = NULL; + tmap_vsw_opt_t *vsw_opt = NULL; + + int32_t softclip_start, softclip_end; + + sams_tmp = tmap_map_sams_init (sams); + tmap_map_sams_realloc (sams_tmp, sams->n); // pre-allocate for same number of mappings + + // set softclip flags, rule-based + tmap_map_util_set_softclip (opt, seq, &softclip_start, &softclip_end); + + // initialize opt + vsw_opt = tmap_vsw_opt_init (opt->score_match, opt->pen_mm, opt->pen_gapo, opt->pen_gape, opt->score_thr); + + // init seqs + orig_query_len = tmap_seq_get_bases_length (seqs [0]); + + // reverse compliment query + vsw = tmap_vsw_init ((uint8_t*) tmap_seq_get_bases(seqs[1])->s, orig_query_len, softclip_end, softclip_start, opt->vsw_type, vsw_opt); + + for (end = 0, i = 0; end < sams->n; ++end) // for each placement + { + tmap_map_sam_t* src_sam = sams->sams + end; // pointer to the receiving sam (last filled element in sams_tmp) + tmap_map_sam_t* dest_sam = sams_tmp->sams + i; + + if (find_alignment_start ( + src_sam, // source: raw (position-only) mapping + dest_sam, // destination: refined (aligned) mapping + seqs, // array of size 4 that contains pre-computed inverse / complement combinations + refseq, // reference server + softclip_start, // is 5' softclip allowed + softclip_end, // is 3' softclip allowed + opt, // tmap parameters + target, // reference data + vsw, // vectorized aligner object + stat + )) + ++i; + } - // update aux data - tmap_map_sam_malloc_aux(s); - switch(s->algo_id) { - case TMAP_MAP_ALGO_MAP1: - (*s->aux.map1_aux) = (*tmp_sam.aux.map1_aux); - break; - case TMAP_MAP_ALGO_MAP2: - (*s->aux.map2_aux) = (*tmp_sam.aux.map2_aux); - break; - case TMAP_MAP_ALGO_MAP3: - (*s->aux.map3_aux) = (*tmp_sam.aux.map3_aux); - break; - case TMAP_MAP_ALGO_MAP4: - (*s->aux.map4_aux) = (*tmp_sam.aux.map4_aux); - break; - case TMAP_MAP_ALGO_MAPVSW: - (*s->aux.map_vsw_aux) = (*tmp_sam.aux.map_vsw_aux); - break; - default: - tmap_bug(); - break; - } + // realloc + tmap_map_sams_realloc (sams_tmp, i); - // key trim the data - if(1 == opt->softclip_key && INT8_MAX != key_base) { - // get the new target - tmap_map_util_keytrim(query, qlen, target, tlen, strand, key_base, s); - } + // free memory + tmap_map_sams_destroy (sams); + tmap_vsw_destroy (vsw); + tmap_vsw_opt_destroy (vsw_opt); - // end repair - if(0 != opt->end_repair) { - tmap_map_util_end_repair(seq, query, qlen, target, tlen, strand, path, refseq, s, opt, 0); - tmap_map_util_end_repair(seq, query, qlen, target, tlen, strand, path, refseq, s, opt, 1); // do 5' end repair?? - } - // TODO: ZZ: If the primer sequences are added, we remove the cigar that corresponds to the sequence, at the same time calculate the score - // of this part of alignment, this will be substracted from the alignment score there. - // to be removed if the other path works. - if (left_added_bases + right_added_bases > 0) { - tmap_map_rmquerybases_cigar(s, left_added_bases, right_added_bases); - } + // sort by max score, then min coordinate - i++; + return sams_tmp; +} - // update start/end - end++; - // start = end; - target = tmp_target; - } +void tmap_map_util_align ( + tmap_refseq_t *refseq, // reference server + tmap_map_sams_t *sams, // mappings to compute alignments for + tmap_seq_t **seqs, // array of size 4 that contains pre-computed inverse / complement combinations + ref_buf_t* target, // reference data + tmap_sw_path_t** path_buf, // buffer for traceback path + int32_t* path_buf_sz, // used portion and allocated size of traceback path. + tmap_sw_param_t* par, // Smith-Waterman scopring parameters + tmap_map_stats_t *stat // statistics +) +{ + uint32_t i; + for (i = 0; i < sams->n; ++i) // for each placement + { + tmap_map_sam_t* dest_sam = sams->sams + i; + compute_alignment ( + dest_sam, // destination: refined (aligned) mapping + seqs, // array of size 4 that contains pre-computed inverse / complement combinations + refseq, // reference server + target, // pointer to the reference cache + path_buf, // buffer for traceback path + path_buf_sz, // used portion and allocated size of traceback path. + par, // Smith-Waterman scoring parameters + stat // statistics + ); + } +} - // realloc - tmap_map_sams_realloc(sams_tmp, i); - - // free memory - tmap_map_sams_destroy(sams); - free(path); - free(target); - tmap_vsw_destroy(vsw); - tmap_vsw_opt_destroy(vsw_opt); - - // sort by max score, then min coordinate - if(1 < sams_tmp->n) { - tmap_sort_introsort(tmap_map_sam_sort_score_coord, sams_tmp->n, sams_tmp->sams); - } +void tmap_map_util_salvage_edge_indels ( + tmap_refseq_t *refseq, // reference server + tmap_map_sams_t *sams, // mappings to compute alignments for + tmap_seq_t **seqs, // array of size 4 that contains pre-computed inverse / complement combinations + tmap_map_opt_t *opt, // tmap parameters + tmap_sw_param_t* par, // Smith-Waterman scopring parameters + ref_buf_t* target, // reference data cache + tmap_sw_path_t** path_buf, // buffer for traceback path + int32_t* path_buf_sz, // used portion and allocated size of traceback path. + tmap_map_stats_t *stat // statistics +) +{ + uint32_t i; + for (i = 0; i < sams->n; ++i) // for each placement + { + tmap_map_sam_t* dest_sam = sams->sams + i; + salvage_long_indel_at_edges ( + dest_sam, // destination: refined (aligned) mapping + seqs, // array of size 4 that contains pre-computed inverse / complement combinations + refseq, // reference server + target, // reference data cache + path_buf, // buffer for traceback path + path_buf_sz, // used portion and allocated size of traceback path. + opt, // tmap options + par, // Smith-Waterman scopring parameters + stat // statistics + ); + } +} - return sams_tmp; +void tmap_map_util_cure_softclips ( + tmap_map_sams_t *sams, // mappings to compute alignments for + tmap_seq_t **seqs // array of size 4 that contains pre-computed inverse / complement combinations +) +{ + uint32_t i; + for (i = 0; i < sams->n; ++i) // for each placement + { + tmap_map_sam_t* dest_sam = sams->sams + i; + extend_softclips_to_read_edges ( + dest_sam, // mapping being adjusted + seqs // 4-element array containing fwd, rec, compl and rev/compl read sequence + ); + } } +void tmap_map_util_trim_key ( + tmap_map_sams_t *sams, // mappings to compute alignments for + tmap_seq_t *seq, // read + tmap_seq_t **seqs, // array of size 4 that contains pre-computed inverse / complement combinations + tmap_refseq_t *refseq, // reference server + ref_buf_t* target, // reference data cache + tmap_map_stats_t *stat // statistics + +) +{ + uint32_t i; + for (i = 0; i < sams->n; ++i) // for each placement + { + tmap_map_sam_t* dest_sam = sams->sams + i; + key_trim_alignment ( + dest_sam, // mapping being trimmed + seqs, // array of size 4 that contains pre-computed inverse / complement combinations + seq, // read + refseq, // reference server + target, // reference data cache + stat // statistics + ); + } +} + +void tmap_map_util_end_repair_bulk ( + tmap_refseq_t *refseq, // reference server + tmap_map_sams_t *sams, // mappings to compute alignments for + tmap_seq_t *seq, // read + tmap_seq_t **seqs, // array of size 4 that contains pre-computed inverse / complement combinations + tmap_map_opt_t *opt, // tmap parameters + ref_buf_t* target, // reference data cache + tmap_sw_path_t** path_buf, // buffer for traceback path + int32_t* path_buf_sz, // used portion and allocated size of traceback path. + tmap_map_stats_t *stat // statistics + +) +{ + uint32_t i; + for (i = 0; i < sams->n; ++i) // for each placement + { + tmap_map_sam_t* dest_sam = sams->sams + i; + end_repair ( + dest_sam, // mapping being trimmed + seqs, // array of size 4 that contains pre-computed inverse / complement combinations + seq, // read + refseq, // reference server + target, // pointer to reference cache control structure + path_buf, // address of the pointer to buffer for traceback path, + path_buf_sz, // pointer to the integer holding allocated size of traceback path. + opt, // tmap options + stat // statistics + ); + } +} + + +tmap_map_sams_t * +tmap_map_util_sw_gen_cigar (tmap_refseq_t* refseq, + tmap_map_sams_t* sams, + tmap_seq_t* seq, + tmap_seq_t** seqs, + tmap_map_opt_t* opt, + tmap_map_stats_t *stat +) +{ + // int32_t start, end; + int32_t i; // index into results buffer; + + tmap_map_sams_t *sams_tmp = NULL; // buffer to receive the alignments + + ref_buf_t target; + target_cache_init (&target); + tmap_sw_path_t *path_buf = NULL; // buffer for traceback path + int32_t path_buf_sz = 0; // used portion and allocated size of traceback path. + tmap_sw_param_t sw_par; + tmap_map_util_populate_sw_par (&sw_par, opt); + + // DK: seems nothing in this function depend on the mappings order + // // sort by strand/chr/pos/score + // tmap_sort_introsort (tmap_map_sam_sort_coord, sams->n, sams->sams); + + // Step 1: fing alignment start point + sams_tmp = tmap_map_util_find_align_starts + ( + refseq, // reference server + sams, // initial rough mapping + seq, // read + seqs, // array of size 4 that contains pre-computed inverse / complement combinations + opt, // tmap parameters + &target, // target cache control structure + stat // statistics + ); + + // Step 2: compute alignment + tmap_map_util_align + ( + refseq, // reference server + sams_tmp, // mappings to compute alignments for + seqs, // array of size 4 that contains pre-computed inverse / complement combinations + &target, // target cache control structure + &path_buf, // buffer for traceback path + &path_buf_sz, // used portion and allocated size of traceback path. + &sw_par, // Smith-Waterman scoring parameters + stat // statistics + ); + + // Step 3: salvage long indels close to the read edges + if (opt->pen_gapl) + tmap_map_util_salvage_edge_indels + ( + refseq, // reference server + sams_tmp, // mappings to compute alignments for + seqs, // array of size 4 that contains pre-computed inverse / complement combinations + opt, // tmap parameters + &sw_par, // Smith-Waterman scoring parameters + &target, // target cache control structure + &path_buf, // buffer for traceback path + &path_buf_sz, // used portion and allocated size of traceback path. + stat // statistics + ); + + + // Step 4: extend alignment to the read edges by adding soft clips + tmap_map_util_cure_softclips + ( + sams_tmp, // mappings to compute alignments for + seqs // array of size 4 that contains pre-computed inverse / complement combinations + ); + + // Step 5: key trim the data + if (opt->softclip_key) + tmap_map_util_trim_key + ( + sams_tmp, // mappings to compute alignments for + seq, // read + seqs, // array of size 4 that contains pre-computed inverse / complement combinations + refseq, // reference server + &target, // target cache control structure + stat // statistics + ); + + // Step 6: end repair + if (opt->end_repair) + tmap_map_util_end_repair_bulk + ( + refseq, // reference server + sams_tmp, // mappings to compute alignments for + seq, // read + seqs, // array of size 4 that contains pre-computed inverse / complement combinations + opt, // tmap parameters + &target, // target cache control structure + &path_buf, // buffer for traceback path + &path_buf_sz, // used portion and allocated size of traceback path. + stat // statistics + ); + + // free memory + // tmap_map_sams_destroy (sams); + free (path_buf); + target_cache_free (&target); + + // sort by max score, then min coordinate + if(1 < sams_tmp->n) + tmap_sort_introsort (tmap_map_sam_sort_score_coord, sams_tmp->n, sams_tmp->sams); + + return sams_tmp; +} + + // TODO: make sure the "longest" read alignment is found int32_t -tmap_map_util_fsw(tmap_seq_t *seq, tmap_map_sams_t *sams, tmap_refseq_t *refseq, +tmap_map_util_fsw (tmap_seq_t *seq, tmap_map_sams_t *sams, tmap_refseq_t *refseq, int32_t bw, int32_t softclip_type, int32_t score_thr, int32_t score_match, int32_t pen_mm, int32_t pen_gapo, - int32_t pen_gape, int32_t fscore, int32_t use_flowgram) -{ - int32_t i, j, k, l; - uint8_t *target = NULL; - int32_t target_mem = 0, target_len = 0; - int32_t was_int = 1; - - tmap_fsw_flowseq_t *fseq = NULL; - tmap_fsw_path_t *path = NULL; - int32_t path_mem = 0, path_len = 0; - tmap_fsw_param_t param; - int32_t matrix[25]; - uint8_t *flow_order = NULL; - int32_t flow_order_len = 0; - uint8_t *key_seq = NULL; - int32_t key_seq_len = 0; - - if(0 == sams->n || NULL == seq->fo || NULL == seq->ks) return 0; - - // flow order - flow_order_len = strlen(seq->fo); - flow_order = tmap_malloc(sizeof(uint8_t)*(flow_order_len+1), "flow_order"); - memcpy(flow_order, seq->fo, flow_order_len); - tmap_to_int((char*)flow_order, flow_order_len); - - // key sequence - key_seq_len = strlen(seq->ks); - key_seq = tmap_malloc(sizeof(uint8_t)*(key_seq_len+1), "key_seq"); - memcpy(key_seq, seq->ks, key_seq_len); - tmap_to_int((char*)key_seq, key_seq_len); + int32_t pen_gape, int32_t fscore, int32_t use_flowgram, tmap_map_stats_t* stat) +{ + int32_t i, j, k, l; + uint8_t *target = NULL; + int32_t target_mem = 0, target_len = 0; + int32_t was_int = 1; + + tmap_fsw_flowseq_t *fseq = NULL; + tmap_fsw_path_t *path = NULL; + int32_t path_mem = 0, path_len = 0; + tmap_fsw_param_t param; + int32_t matrix [25]; + uint8_t *flow_order = NULL; + int32_t flow_order_len = 0; + uint8_t *key_seq = NULL; + int32_t key_seq_len = 0; + + if (0 == sams->n || NULL == seq->fo || NULL == seq->ks) + return 0; - // generate the alignment parameters - param.matrix = matrix; - param.band_width = 0; - param.offset = TMAP_MAP_OPT_FSW_OFFSET; // this sets the hp difference - __tmap_fsw_gen_ap1(param, score_match, pen_mm, pen_gapo, pen_gape, fscore); + // flow order + flow_order_len = strlen (seq->fo); + flow_order = tmap_malloc (sizeof (uint8_t) * (flow_order_len + 1), "flow_order"); + memcpy (flow_order, seq->fo, flow_order_len); + tmap_to_int ((char*) flow_order, flow_order_len); + + // key sequence + key_seq_len = strlen (seq->ks); + key_seq = tmap_malloc (sizeof (uint8_t) * (key_seq_len+1), "key_seq"); + memcpy (key_seq, seq->ks, key_seq_len); + tmap_to_int ((char*) key_seq, key_seq_len); + + // generate the alignment parameters + param.matrix = matrix; + param.band_width = 0; + param.offset = TMAP_MAP_OPT_FSW_OFFSET; // this sets the hp difference + __tmap_fsw_gen_ap1 (param, score_match, pen_mm, pen_gapo, pen_gape, fscore); + + was_int = tmap_seq_is_int (seq); + if (0 == tmap_seq_is_int(seq)) + tmap_seq_to_int(seq); + + // get flow sequence + fseq = tmap_fsw_flowseq_from_seq (NULL, seq, flow_order, flow_order_len, key_seq, key_seq_len, use_flowgram); + + if (!fseq) + { + free (flow_order); + free (key_seq); + return 0; + } - was_int = tmap_seq_is_int(seq); - if(0 == tmap_seq_is_int(seq)) { - tmap_seq_to_int(seq); - } + // go through each hit + for (i = 0; i < sams->n; ++i) + { + tmap_map_sam_t *s = &sams->sams [i]; + uint32_t ref_start, ref_end; + // get the reference end position + // NB: soft-clipping at the start may cause ref_start to be moved + param.band_width = 0; + ref_start = ref_end = s->pos + 1; + for (j = 0; j < s->n_cigar; ++j) + { + int32_t op, op_len; + + op = TMAP_SW_CIGAR_OP (s->cigar [j]); + op_len = TMAP_SW_CIGAR_LENGTH (s->cigar [j]); + + switch(op) + { + case BAM_CMATCH: + ref_end += op_len; + break; + case BAM_CDEL: + if (param.band_width < op_len) + param.band_width += op_len; + ref_end += op_len; + break; + case BAM_CINS: + if (param.band_width < op_len) + param.band_width += op_len; + break; + case BAM_CSOFT_CLIP: + if (0 == j) + { + if (ref_start <= op_len) + ref_start = 1; + else + ref_start = ref_start - op_len; + } + else + ref_end += op_len; + break; + default: + // ignore + break; + } + } + ref_end--; // NB: since we want this to be one-based + + // check bounds + if (ref_start < 1) + ref_start = 1; + if (refseq->annos [s->seqid].len < ref_end) + ref_end = refseq->annos [s->seqid].len; + else if (ref_end < 1) + ref_end = 1; + + // get the target sequence + target_len = ref_end - ref_start + 1; + if(target_mem < target_len) + { + target_mem = target_len; + tmap_roundup32 (target_mem); + target = tmap_realloc (target, sizeof (uint8_t) * target_mem, "target"); + } + target_len = tmap_refseq_subseq (refseq, ref_start + refseq->annos [s->seqid].offset, target_len, target); + /* + // NB: IUPAC codes are turned into mismatches + if(NULL == tmap_refseq_subseq2(refseq, sams->sams[end].seqid+1, start_pos, end_pos, target, 1, NULL)) { + tmap_bug(); + } + */ - // get flow sequence - fseq = tmap_fsw_flowseq_from_seq(NULL, seq, flow_order, flow_order_len, key_seq, key_seq_len, use_flowgram); + if (1 == s->strand) // reverse compliment + tmap_reverse_compliment_int(target, target_len); - if(NULL == fseq) { - free(flow_order); - free(key_seq); - return 0; - } + // add to the band width + param.band_width += 2 * bw; - // go through each hit - for(i=0;in;i++) { - tmap_map_sam_t *s = &sams->sams[i]; - uint32_t ref_start, ref_end; + // make sure we have enough memory for the path + while (path_mem <= target_len + fseq->num_flows) // lengthen the path + { + path_mem = target_len + fseq->num_flows + 1; + tmap_roundup32 (path_mem); + path = tmap_realloc (path, sizeof (tmap_fsw_path_t) * path_mem, "path"); + } - // get the reference end position - // NB: soft-clipping at the start may cause ref_start to be moved - param.band_width = 0; - ref_start = ref_end = s->pos + 1; - for(j=0;jn_cigar;j++) { - int32_t op, op_len; + /* + fprintf(stderr, "strand=%d\n", s->strand); + fprintf(stderr, "ref_start=%d ref_end=%d\n", ref_start, ref_end); + fprintf(stderr, "base_calls:\n"); + for(j=0;jnum_flows;j++) { + for(k=0;kbase_calls[j];k++) { + fputc("ACGTN"[fseq->flow_order[j % fseq->flow_order_len]], stderr); + } + } + fputc('\n', stderr); + fprintf(stderr, "target:\n"); + for(j=0;jflow_order_len;j++) { + fputc("ACGTN"[fseq->flow_order[j]], stderr); + } + fputc('\n', stderr); + */ + + // re-align + s->ascore = s->score; + path_len = path_mem; + //fprintf(stderr, "old score=%d\n", s->score); + switch (softclip_type) + { + case TMAP_MAP_OPT_SOFT_CLIP_ALL: + s->score = tmap_fsw_clipping_core (target, target_len, fseq, ¶m, + 1, 1, s->strand, path, &path_len); + break; + case TMAP_MAP_OPT_SOFT_CLIP_LEFT: + s->score = tmap_fsw_clipping_core (target, target_len, fseq, ¶m, + 1, 0, s->strand, path, &path_len); + break; + case TMAP_MAP_OPT_SOFT_CLIP_RIGHT: + s->score = tmap_fsw_clipping_core (target, target_len, fseq, ¶m, + 0, 1, s->strand, path, &path_len); + break; + case TMAP_MAP_OPT_SOFT_CLIP_NONE: + s->score = tmap_fsw_clipping_core (target, target_len, fseq, ¶m, + 0, 0, s->strand, path, &path_len); + break; + default: + tmap_error("soft clipping type was not recognized", Exit, OutOfRange); + break; + } + s->score_subo = INT32_MIN; + //fprintf(stderr, "new score=%d path_len=%d\n", s->score, path_len); + + if (0 < path_len) + { // update + /* + for(j=0;jscore = (int32_t)((s->score + 99.99) / 100.0); + + // position + s->pos = ref_start - 1; // zero-based + // NB: must be careful of leading insertions and strandedness + if (0 == s->strand) + { + if (0 <= path [path_len - 1].j) + s->pos += (path [path_len - 1].j); + } + else + { + if (path [0].j < target_len) + s->pos += target_len - path [0].j - 1; + } + + if (refseq->len < s->pos) + tmap_bug (); + + // new cigar + free (s->cigar); + s->cigar = tmap_fsw_path2cigar (path, path_len, &s->n_cigar, 1); + + // reverse the cigar + if (1 == s->strand) + for(i=0; i < (s->n_cigar >> 1); i++) + { + uint32_t t = s->cigar [i]; + s->cigar [i] = s->cigar [s->n_cigar - i - 1]; + s->cigar [s->n_cigar - i - 1] = t; + } - op = TMAP_SW_CIGAR_OP(s->cigar[j]); - op_len = TMAP_SW_CIGAR_LENGTH(s->cigar[j]); + int32_t skipped_start, skipped_end; + skipped_start = skipped_end = 0; + + // soft-clipping + if (0 < path [path_len - 1].i) // skipped beginning flows + // get the number of bases to clip + for (j = 0; j < path [path_len - 1].i; j++) + skipped_start += fseq->base_calls[j]; + + if (path [0].i + 1 < fseq->num_flows) // skipped ending flows + // get the number of bases to clip + for (j = path [0].i + 1; j < fseq->num_flows; j++) + skipped_end += fseq->base_calls [j]; + + if (1 == s->strand) // swap + { + k = skipped_start; + skipped_start = skipped_end; + skipped_end = k; + } + if (0 < skipped_start) // start soft clip + { + s->cigar = tmap_realloc (s->cigar, sizeof (uint32_t) * (1 + s->n_cigar), "s->cigar"); + for (l = s->n_cigar-1; 0 <= l; l--) + s->cigar [l + 1] = s->cigar [l]; + + TMAP_SW_CIGAR_STORE (s->cigar[0], BAM_CSOFT_CLIP, skipped_start); + s->n_cigar++; + } + if (0 < skipped_end) // end soft clip + { + s->cigar = tmap_realloc (s->cigar, sizeof (uint32_t) * (1 + s->n_cigar), "s->cigar"); + s->cigar [s->n_cigar] = (skipped_end << 4) | 4; + s->n_cigar++; + } + } + } + // free + free (target); + free (path); + free (flow_order); + free (key_seq); + + if (0 == was_int) + tmap_seq_to_char(seq); + + return 1; +} + + +int32_t tmap_map_util_remove_5_prime_softclip +( + tmap_refseq_t *refseq, // reference server + tmap_map_sam_t *dest_sam, // mappings to fix + tmap_seq_t *seq, // read + tmap_seq_t **seqs, // array of size 4 that contains pre-computed inverse / complement combinations + ref_buf_t* target, // reference data cache + tmap_sw_path_t** path_buf, // buffer for traceback path + int32_t* path_buf_sz, // used portion and allocated size of traceback path. + tmap_sw_param_t* par, // Smith-Waterman scopring parameters + tmap_map_opt_t *opt, // tmap parameters + tmap_map_stats_t *stat // statistics +) +{ + + if (dest_sam->strand == 0 && (dest_sam->n_cigar == 0 || TMAP_SW_CIGAR_OP (dest_sam->cigar [0]) != BAM_CSOFT_CLIP)) + return 0; + if (dest_sam->strand == 1 && (dest_sam->n_cigar == 0 || TMAP_SW_CIGAR_OP (dest_sam->cigar [dest_sam->n_cigar - 1]) != BAM_CSOFT_CLIP)) + return 0; + // if we're here, there is a soft clip on 5' + + // if (0 == strcmp (seq->data.sam->name->s, "K0BK3:03674:08750")) + // printf ("Here"); + uint8_t* orig_query = (uint8_t*) tmap_seq_get_bases (seqs [dest_sam->strand])->s; // forward genomic strand + int32_t orig_query_len = tmap_seq_get_bases_length (seqs [0]); + + int32_t sc_len = TMAP_SW_CIGAR_LENGTH ((dest_sam->strand == 0) ? (dest_sam->cigar [0]) : (dest_sam->cigar [dest_sam->n_cigar - 1])); + int32_t max_ins_equiv = (opt->pen_gape != 0) ? ((opt->score_match * sc_len - opt->pen_gapo) / opt->pen_gape) : (opt->score_match * sc_len - opt->pen_gapo); + if (max_ins_equiv < 0) + max_ins_equiv = 0; + int32_t target_len = sc_len + max_ins_equiv; + + int32_t start_pos, end_pos; + + if (dest_sam->strand == 0) + { + // 5' is a beginning of the alignment, align forward ref to forward read in no-clipping mode + // target + start_pos = (dest_sam->pos > target_len) ? (dest_sam->pos - target_len + 1) : 1; + end_pos = dest_sam->pos; + if (start_pos >= end_pos) + { + // use Ins in place of SC + TMAP_SW_CIGAR_STORE (dest_sam->cigar [0], BAM_CINS, sc_len); + dest_sam->score -= opt->pen_gapo + opt->pen_gape*sc_len; + dest_sam->result.query_start = 0; + } + else + { + cache_target (target, refseq, dest_sam->seqid + 1, start_pos, end_pos); + // path memory + if (*path_buf_sz <= target->data_len + sc_len) + { // lengthen the path + *path_buf_sz = target->data_len + sc_len; + tmap_roundup32 (*path_buf_sz); + *path_buf = tmap_realloc (*path_buf, sizeof (tmap_sw_path_t) * *path_buf_sz, "path_buf"); + } + // align softclipped part of the query + int32_t path_len; + int32_t add_score = tmap_sw_clipping_core2 ( + target->data, + target->data_len, + orig_query, + sc_len, + par, + 1, 0, // only allow the start of the target to be skipped + 0, 0, // do not allow any softclips + *path_buf, + &path_len, + 0); + + // build the cigar + int32_t addition_len; + uint32_t* addition = tmap_sw_path2cigar (*path_buf, path_len, &addition_len); + if (addition_len == 0) // alignment failure - use Ins + { + // use Ins in place of SC + TMAP_SW_CIGAR_STORE (dest_sam->cigar [0], BAM_CINS, sc_len); + dest_sam->score -= opt->pen_gapo + opt->pen_gape*sc_len; + dest_sam->result.query_start = 0; + return 1; + } + assert (addition); + assert (dest_sam->n_cigar > 1); + // compute and apply pos, target_len amd alignment box adjustment + int32_t cp, addition_ref_len = 0; + for (cp = 0; cp != addition_len; ++cp) + { + int32_t op = TMAP_SW_CIGAR_OP (addition [cp]); + int32_t oplen = TMAP_SW_CIGAR_LENGTH (addition [cp]); + if (op == BAM_CMATCH || op == BAM_CDEL) + addition_ref_len += oplen; + } + + //int32_t pos_adj = 0; // DK: I do not quite understand this. Modeled after position computing in 'raw' alignment. + // DK: end-repair does not do this adjustment + //if ((*path_buf) [0].ctype == TMAP_SW_FROM_I) + // pos_adj++; // TODO: is this correct? + + stat->num_5_softclips [dest_sam->strand] ++; + stat->bases_5_softclips_qry [dest_sam->strand] += dest_sam->result.query_start; + stat->bases_5_softclips_ref [dest_sam->strand] += addition_ref_len; + stat->score_5_softclips_total [dest_sam->strand] += add_score; + + assert (addition_ref_len <= dest_sam->pos); + dest_sam->pos -= addition_ref_len; // - pos_adj; + dest_sam->target_len += addition_ref_len; + dest_sam->result.target_end += addition_ref_len; + dest_sam->result.query_start = 0; + + + //tmap_log_record_begin (); + //tmap_map_log_text_align (" After 5' unclip :\n", addition, addition_len, (const char *) orig_query, orig_query_len, !dest_sam->strand, (const char*) (target->data + (target->data_len - addition_ref_len)), dest_sam->pos); + //tmap_log_record_end (); + + // merge addition with the cigar + // check if last op of addition matches first op of orig cigar; merge ops if yes + int32_t old_shift = addition_len - 1; + int32_t addition_last_op = TMAP_SW_CIGAR_OP (addition [addition_len - 1]); + int32_t old_first_nonsc_op = TMAP_SW_CIGAR_OP (dest_sam->cigar [1]); + if (addition_last_op == old_first_nonsc_op) + { + int32_t merged_len = TMAP_SW_CIGAR_LENGTH (addition [addition_len - 1]) + TMAP_SW_CIGAR_LENGTH (dest_sam->cigar [1]); + TMAP_SW_CIGAR_STORE (addition [addition_len - 1], addition_last_op, merged_len); + --old_shift; + } + int32_t new_cigar_len = dest_sam->n_cigar + old_shift; + if (new_cigar_len > dest_sam->n_cigar) + dest_sam->cigar = tmap_realloc (dest_sam->cigar, new_cigar_len * sizeof (*(dest_sam->cigar)), "cigar"); + if (old_shift > 0) + memmove (dest_sam->cigar + old_shift, dest_sam->cigar, dest_sam->n_cigar * sizeof (*(dest_sam->cigar))); + if (old_shift < 0) + memmove (dest_sam->cigar, dest_sam->cigar - old_shift, (dest_sam->n_cigar + old_shift) * sizeof (*(dest_sam->cigar))); + memcpy (dest_sam->cigar, addition, addition_len * sizeof (*(dest_sam->cigar))); + dest_sam->n_cigar = new_cigar_len; + dest_sam->score += add_score; + free (addition); + + } + } + else + { + // 5' is an end of the alignment, align foward ref to reverse read in no-clipping mode + start_pos = dest_sam->pos + dest_sam->target_len + 1; + end_pos = start_pos + target_len - 1; + if (end_pos > refseq->annos [dest_sam->seqid].len) + end_pos = refseq->annos [dest_sam->seqid].len; + if (start_pos >= end_pos) + { + // use Ins in place of SC + TMAP_SW_CIGAR_STORE (dest_sam->cigar [dest_sam->n_cigar - 1], BAM_CINS, sc_len); + dest_sam->score -= opt->pen_gapo + opt->pen_gape*sc_len; + dest_sam->result.query_end = orig_query_len - 1; + } + else + { + assert (start_pos < refseq->annos [dest_sam->seqid].len); + //if (start_pos >= end_pos) + // printf ("here"); + assert (start_pos < end_pos); + cache_target (target, refseq, dest_sam->seqid + 1, start_pos, end_pos); + // path memory + if (*path_buf_sz <= target->data_len + sc_len) + { // lengthen the path + *path_buf_sz = target->data_len + sc_len; + tmap_roundup32 (*path_buf_sz); + *path_buf = tmap_realloc (*path_buf, sizeof (tmap_sw_path_t) * *path_buf_sz, "path_buf"); + } + // align softclipped part of the query + int32_t path_len; + int32_t add_score = tmap_sw_clipping_core2 ( + target->data, + target->data_len, + orig_query, + sc_len, + par, + 0, 1, // only allow the end of the target to be skipped + 0, 0, // do not allow any softclips + *path_buf, + &path_len, + 0); + + // build the cigar + int32_t addition_len; + uint32_t* addition = tmap_sw_path2cigar (*path_buf, path_len, &addition_len); + if (addition_len == 0) // alignment failure - use Ins + { + // use Ins in place of SC + TMAP_SW_CIGAR_STORE (dest_sam->cigar [dest_sam->n_cigar - 1], BAM_CINS, sc_len); + dest_sam->score -= opt->pen_gapo + opt->pen_gape*sc_len; + dest_sam->result.query_end = orig_query_len - 1; + return 1; + } + assert (addition); + + // compute and apply pos, target_len amd alignment box adjustment + int32_t cp, addition_ref_len = 0; + for (cp = 0; cp != addition_len; ++cp) + { + int32_t op = TMAP_SW_CIGAR_OP (addition [cp]); + int32_t oplen = TMAP_SW_CIGAR_LENGTH (addition [cp]); + if (op == BAM_CMATCH || op == BAM_CDEL) + addition_ref_len += oplen; + } + + stat->num_5_softclips [dest_sam->strand] ++; + stat->bases_5_softclips_qry [dest_sam->strand] += dest_sam->result.query_start; + stat->bases_5_softclips_ref [dest_sam->strand] += addition_ref_len; + stat->score_5_softclips_total [dest_sam->strand] += add_score; + + assert (dest_sam->pos + addition_ref_len <= refseq->annos [dest_sam->seqid].len); + dest_sam->target_len += addition_ref_len; + dest_sam->result.target_end += addition_ref_len; + dest_sam->result.query_end = orig_query_len - 1; + // merge addition with the cigar + int32_t addition_pos = dest_sam->n_cigar - 1; + assert (addition_len); + assert (dest_sam->n_cigar > 1); + int32_t addition_first_op = TMAP_SW_CIGAR_OP (addition [0]); + int32_t old_last_nonsc_op = TMAP_SW_CIGAR_OP (dest_sam->cigar [dest_sam->n_cigar - 2]); + if (addition_first_op == old_last_nonsc_op) + { + int32_t merged_len = TMAP_SW_CIGAR_LENGTH (addition [0]) + TMAP_SW_CIGAR_LENGTH (dest_sam->cigar [dest_sam->n_cigar - 2]); + TMAP_SW_CIGAR_STORE (addition [0], addition_first_op, merged_len); + --addition_pos; + if (addition_first_op == BAM_CINS || addition_first_op == BAM_CDEL) + add_score -= opt->pen_gapo; + } + int32_t new_cigar_len = addition_len + addition_pos; + if (new_cigar_len > dest_sam->n_cigar) + dest_sam->cigar = tmap_realloc (dest_sam->cigar, new_cigar_len * sizeof (*(dest_sam->cigar)), "cigar"); + memcpy (dest_sam->cigar + addition_pos, addition, addition_len * sizeof (*(dest_sam->cigar))); + dest_sam->n_cigar = new_cigar_len; + dest_sam->score += add_score; + free (addition); + + } + } + + return 1; +} + +static uint8_t bin_cigar_to_str (uint32_t* cigar, int32_t n_cigar, char* dest, uint32_t dest_sz) +{ + uint32_t* cent = cigar + n_cigar; + char* dp = dest; + uint32_t incr; + for (; cigar != cent; ++cigar, ++dest) + { + incr = snprintf (dp, dest_sz - (dp - dest), "%d", TMAP_SW_CIGAR_LENGTH (*cigar)); + if (dest_sz - (dp - dest) < incr + 1) + return 0; + dp += incr; + if (dest_sz - (dp - dest) < 2) + return 0; + *(dp++) = BAM_CIGAR_STR [TMAP_SW_CIGAR_OP (*cigar)]; + } + if (dp - dest >= dest_sz) + return 0; + *dp = 0; + return 1; +} + +// checks that alignment score is close enough to the recorded one +// checks if cigar consistent with read size and target_len +// checks if the target boundaries are right +// checks if alignment box consistent with cigar +void cigar_sanity_check +( + tmap_refseq_t *refseq, // reference server + tmap_map_sam_t *sam, // mapping to check + tmap_seq_t *seq, // read + tmap_seq_t **seqs, // array of size 4 that contains pre-computed inverse / complement combinations + ref_buf_t* target, // reference data cache + tmap_map_opt_t *opt // tmap options (for this stage) +) +{ - switch(op) { + cache_target (target, refseq, sam->seqid + 1, sam->pos + 1, sam->pos + sam->target_len); + uint8_t* ref = target->data; + int32_t ref_len = target->data_len; + tmap_string_t* seq_str = seqs [sam->strand]->data.sam->seq; + char* qry = seq_str->s; + int32_t qry_len = seq_str ->l; + + uint32_t cigar_str_sz = 4 * sam->n_cigar + 1; + char* cigar_str = alloca (cigar_str_sz); + if (!bin_cigar_to_str (sam->cigar, sam->n_cigar, cigar_str, cigar_str_sz)) + tmap_bug (); + + if (sam->result.target_start != 0) + tmap_failure ("Alignment sanity check error on read %s:\n alignment box start is %d, should be 0", seq->data.sam->name->s, sam->result.target_start); + + uint32_t qry_pos = 0, ref_pos = 0; + int32_t al_score = 0, nogap_score = 0, score_chg; + uint32_t *opptr, *sent; + uint32_t op, prev_op = 0, oplen, p; // prev_op to 0 to alleviate gcc's jealousy. It thinks it may be uninitialized downstream (logic guarantees otherwise, but it can see only syntax) + for (opptr = sam->cigar, sent = opptr + sam->n_cigar; opptr != sent; ++opptr) + { + op = TMAP_SW_CIGAR_OP (*opptr); + oplen = TMAP_SW_CIGAR_LENGTH (*opptr); + if (opptr == sam->cigar && op != BAM_CSOFT_CLIP && sam->result.query_start != 0) + tmap_conderr ((opt->cigar_sanity_check >= TMAP_MAP_SANITY_ERR_CONTENT), "Alignment sanity check error on read %s:\n query offset is %d while cigar (%s) does not start with softclip", seq->data.sam->name->s, sam->result.query_start, cigar_str); + switch (op) + { case BAM_CMATCH: - ref_end += op_len; - break; - case BAM_CDEL: - if(param.band_width < op_len) param.band_width += op_len; - ref_end += op_len; - break; + for (p = 0; p != oplen; ++p) + { + if (ref_pos >= ref_len) + tmap_conderr ((opt->cigar_sanity_check >= TMAP_MAP_SANITY_ERR_CONTENT), "Alignment sanity check error on read %s:\n cigar (%s) takes more reference positions than target length (%d)", seq->data.sam->name->s, cigar_str, ref_len); + if (qry_pos >= qry_len) + tmap_conderr ((opt->cigar_sanity_check >= TMAP_MAP_SANITY_ERR_CONTENT), "Alignment sanity check error on read %s:\n cigar (%s) takes more query positions than query length (%d)", seq->data.sam->name->s, cigar_str, qry_len); + score_chg = (ref [ref_pos] == qry [qry_pos])?(opt->score_match):(-opt->pen_mm); + al_score += score_chg; + nogap_score += score_chg; + ++qry_pos; + ++ref_pos; + } + break; case BAM_CINS: - if(param.band_width < op_len) param.band_width += op_len; - break; + if (qry_pos + oplen > qry_len) + tmap_conderr ((opt->cigar_sanity_check >= TMAP_MAP_SANITY_ERR_CONTENT), "Alignment sanity check error on read %s:\n cigar (%s) takes more query positions than query length (%d)", seq->data.sam->name->s, cigar_str, qry_len); + al_score -= (opt->pen_gapo + opt->pen_gape*oplen); + qry_pos += oplen; + break; + case BAM_CDEL: + if (ref_pos + oplen > ref_len) + tmap_conderr ((opt->cigar_sanity_check >= TMAP_MAP_SANITY_ERR_CONTENT), "Alignment sanity check error on read %s:\n cigar (%s) takes more reference positions than target length (%d)", seq->data.sam->name->s, cigar_str, ref_len); + al_score -= (opt->pen_gapo + opt->pen_gape*oplen); + ref_pos += oplen; + break; case BAM_CSOFT_CLIP: - if(0 == j) { - if(ref_start <= op_len) { - ref_start = 1; - } - else { - ref_start = ref_start - op_len; - } - } - else ref_end += op_len; - break; + if (qry_pos + oplen > qry_len) + tmap_conderr ((opt->cigar_sanity_check >= TMAP_MAP_SANITY_ERR_CONTENT), "Alignment sanity check error on read %s:\n cigar (%s) takes more query positions than query length (%d)", seq->data.sam->name->s, cigar_str, qry_len); + qry_pos += oplen; + if (opptr == sam->cigar) // first operation + { + if (qry_pos != sam->result.query_start) + tmap_conderr ((opt->cigar_sanity_check >= TMAP_MAP_SANITY_ERR_CONTENT), "Alignment sanity check error on read %s:\n cigar (%s) has starting softclip of size %d, while query start is %d", seq->data.sam->name->s, cigar_str, sam->result.query_start, oplen); + } + else if (opptr + 1 == sent) // last operation + { + if (sam->result.query_end + 1 + oplen != qry_len) + tmap_conderr ((opt->cigar_sanity_check >= TMAP_MAP_SANITY_ERR_CONTENT), "Alignment sanity check error on read %s:\n cigar (%s) has ending softclip of size %d, while query alignment box ends %d bases before end", seq->data.sam->name->s, cigar_str, oplen, qry_len - (sam->result.query_end + 1)); + } + else // SOFTCLIP not at the edge + tmap_conderr ((opt->cigar_sanity_check >= TMAP_MAP_SANITY_ERR_CONTENT), "Alignment sanity check error on read %s:\n cigar (%s) has internal softclip at position %d", seq->data.sam->name->s, cigar_str, opptr - sam->cigar); + break; default: - // ignore - break; - } - } - ref_end--; // NB: since we want this to be one-based + tmap_bug (); + } + if (opptr != sam->cigar && prev_op == op) + tmap_conderr ((opt->cigar_sanity_check >= TMAP_MAP_SANITY_ERR_CONTENT), "Alignment sanity check error on read %s:\n cigar (%s) have consecutive cigar operations of the same type at position %d", seq->data.sam->name->s, cigar_str, opptr - sam->cigar - 1); + prev_op = op; + } + if (qry_pos != qry_len) + tmap_conderr ((opt->cigar_sanity_check >= TMAP_MAP_SANITY_ERR_CONTENT), "Alignment sanity check error on read %s:\n cigar (%s) takes %d query bases while query len is %d", seq->data.sam->name->s, cigar_str, qry_pos, qry_len); + if (sam->n_cigar != 0 && prev_op != BAM_CSOFT_CLIP && sam->result.query_end + 1 != qry_len) + tmap_conderr ((opt->cigar_sanity_check >= TMAP_MAP_SANITY_ERR_CONTENT), "Alignment sanity check error on read %s:\n alignment box's query end (%d) does not match (query_len-1)(%d)", seq->data.sam->name->s, cigar_str, sam->result.query_end, qry_len-1); + if (ref_pos != sam->target_len) + tmap_conderr ((opt->cigar_sanity_check >= TMAP_MAP_SANITY_ERR_CONTENT), "Alignment sanity check error on read %s:\n cigar (%s) takes %d reference bases while target len is %d", seq->data.sam->name->s, cigar_str, ref_pos, ref_len); + if (ref_pos != sam->result.target_end + 1) + tmap_conderr ((opt->cigar_sanity_check >= TMAP_MAP_SANITY_ERR_CONTENT), "Alignment sanity check error on read %s:\n cigar target size (%d) does not match (alignment box + 1)(%d)", seq->data.sam->name->s, ref_pos, sam->result.target_end + 1); + +#define TMIN(X, Y) (((X) < (Y)) ? (X) : (Y)) +#define TMAX(X, Y) (((Y) < (X)) ? (X) : (Y)) + + // check that original alignment is consistent with post-processed one + // check if alignments differ + if (sam->n_cigar != sam->n_orig_cigar || sam->pos != sam->orig_pos || 0 != memcmp (sam->cigar, sam->orig_cigar, sam->n_cigar * sizeof (*(sam->cigar)))) + { +#define TOTAL_OVERLAP_CHECK +#ifdef TOTAL_OVERLAP_CHECK + { + #define MIN_OVERLAP_FACTOR 2 + // compute total overlap length and total match lengths + // use mergewalk to avoid quadratic runtime + int32_t r1_off = sam->orig_pos, q1_off = 0; // reference and query offset at current match in cigar1 (original) + int32_t r2_off = sam->pos, q2_off = 0; // reference and query offset at current match in cigar2 (post-processed) + uint32_t *opptr1 = sam->orig_cigar, *sent1 = opptr1 + sam->n_orig_cigar; // current operation pointer and sentinel for interating over cigar1 (original) + uint32_t *opptr2, *sent2 = sam->cigar + sam->n_cigar; // current operation pointer and sentinel for interating over cigar2 (post-processed) + uint32_t *tail2 = sam->cigar; // index in cigar2 (new) from which to start looking for overlap with current match in cigar1 (orig) + int32_t tail2_q_off = 0, tail2_r_off = r2_off; // match offsets at the beginning of tail2 match + int32_t op1, oplen1, op2, oplen2; // current cigar operations and operation lengths + int32_t match1_tot = 0, overlap_tot = 0; + int32_t stop, tail2_recorded; // flags for inner loop + for (; opptr1 != sent1; ++opptr1) // iterate over cigar1 (once) + { + op1 = TMAP_SW_CIGAR_OP (*opptr1), oplen1 = TMAP_SW_CIGAR_LENGTH (*opptr1); + switch (op1) + { + case BAM_CINS: + q1_off += oplen1; + break; + case BAM_CDEL: + r1_off += oplen1; + break; + case BAM_CSOFT_CLIP: + q1_off += oplen1; + break; + case BAM_CMATCH: + // advance along cigar 2 from last remembered 'tail2' position until overlap with op1 is possible (until beg2 < end1) + stop = 0, tail2_recorded = 0; + for (opptr2 = tail2, q2_off = tail2_q_off, r2_off = tail2_r_off; opptr2 != sent2 && !stop; ++opptr2) + { + op2 = TMAP_SW_CIGAR_OP (*opptr2), oplen2 = TMAP_SW_CIGAR_LENGTH (*opptr2); + switch (op2) + { + case BAM_CINS: + q2_off += oplen2; + break; + case BAM_CDEL: + r2_off += oplen2; + break; + case BAM_CSOFT_CLIP: + q2_off += oplen2; + break; + case BAM_CMATCH: + // check if overlap still possible (if beg2 < end1), break if not + if (r2_off >= r1_off + oplen1) + { + stop = 1; + break; + } + // remember where end2 overruned end1 as 'tail2' - these reads can potentially overlap next cigar1 match + if (!tail2_recorded && r2_off + oplen2 > r1_off + oplen1) + { + tail2 = opptr2; + tail2_r_off = r2_off; + tail2_q_off = q2_off; + tail2_recorded = 1; + } + // if diagonal is same, compute overlap and add to total + if (r2_off - q2_off == r1_off - q1_off) + { + int32_t largest_beg = TMAX (r2_off, r1_off); + int32_t smallest_end = TMIN (r2_off + oplen2, r1_off + oplen1); + int32_t overlap = smallest_end - largest_beg; + if (overlap > 0) + overlap_tot += overlap; + } + r2_off += oplen2; + q2_off += oplen2; + break; + default: + tmap_bug (); + } + } + q1_off += oplen1; + r1_off += oplen1; + match1_tot += oplen1; + break; + default: + tmap_bug (); + } + } + // now compute post-processed alignment total match length - it is not trivial to do this in above loop as each match in pp cigar may be seen several times + int32_t match2_tot = 0; + for (opptr2 = sam->cigar, sent2 = opptr2 + sam->n_cigar; opptr2 != sent2; ++opptr2) + if (TMAP_SW_CIGAR_OP (*opptr2) == BAM_CMATCH) + match2_tot += TMAP_SW_CIGAR_LENGTH (*opptr2); + { + if (overlap_tot * MIN_OVERLAP_FACTOR < TMIN (match1_tot, match2_tot)) + { + uint32_t orig_cigar_str_sz = 4 * sam->n_orig_cigar + 1; + char* orig_cigar_str = alloca (orig_cigar_str_sz); + if (!bin_cigar_to_str (sam->orig_cigar, sam->n_orig_cigar, orig_cigar_str, orig_cigar_str_sz)) + tmap_bug (); + tmap_conderr ((opt->cigar_sanity_check >= TMAP_MAP_SANITY_ERR_CONTENT_ALIGN), "Alignment sanity check error on read %s:\n post-processed alignment %s at position %d of %s (%s) has insufficient overlap with the original one %s at position %d: overlap=%d, orig_match_len=%d, pp_match_len=%d", seq->data.sam->name->s, cigar_str, sam->pos, refseq->annos [sam->seqid].name->s, (sam->strand?"rev":"fwd"), orig_cigar_str, sam->orig_pos, overlap_tot, match1_tot, match2_tot); + } +#if DEBUG_ALIGNMENT_OVERLAP + else + { + uint32_t orig_cigar_str_sz = 4 * sam->n_orig_cigar + 1; + char* orig_cigar_str = alloca (orig_cigar_str_sz); + if (!bin_cigar_to_str (sam->orig_cigar, sam->n_orig_cigar, orig_cigar_str, orig_cigar_str_sz)) + tmap_bug (); + tmap_conderr (0, "Alignment sanity check Ok on read %s:\n post-processed alignment %s at position %d has sufficient overlap with the original one %s at position %d: overlap=%d, match_len=%d", seq->data.sam->name->s, cigar_str, sam->pos, orig_cigar_str, sam->orig_pos, overlap_tot, match1_tot); + } +#endif + } + } +#endif +// #define LONG_MATCH_OVERLAP_CHECK +#ifdef LONG_MATCH_OVERLAP_CHECK + { + // at least one long match should overlap significantly + #define MIN_LONG_LEN 30 + #define MIN_CHECKED_MATCH_LEN 16 + #define MIN_OVERLAP_FACTOR 2 + int32_t r1_off = sam->orig_pos, r2_off, q1_off = 0, q2_off; + uint32_t *opptr1, *sent1, *opptr2, *sent2; + int32_t op1, oplen1, op2, oplen2; + int32_t has_overlap = 0, has_long = 0; + for (opptr1 = sam->orig_cigar, sent1 = opptr1 + sam->n_orig_cigar; opptr1 != sent1 && !has_overlap; ++opptr1) + { + op1 = TMAP_SW_CIGAR_OP (*opptr1); + oplen1 = TMAP_SW_CIGAR_LENGTH (*opptr1); + switch (op1) + { + case BAM_CINS: + q1_off += oplen1; + break; + case BAM_CDEL: + r1_off += oplen1; + break; + case BAM_CSOFT_CLIP: + q1_off += oplen1; + break; + case BAM_CMATCH: + { + if (oplen1 >= MIN_LONG_LEN) + has_long = 1; + if (oplen1 > MIN_CHECKED_MATCH_LEN) + { + r2_off = sam->pos, q2_off = 0, has_overlap = 0; + for (opptr2 = sam->cigar, sent2 = opptr2 + sam->n_cigar ; opptr2 != sent2 && !has_overlap; ++opptr2) + { + op2 = TMAP_SW_CIGAR_OP (*opptr2); + oplen2 = TMAP_SW_CIGAR_LENGTH (*opptr2); + switch (op2) + { + case BAM_CINS: + q2_off += oplen2; + break; + case BAM_CDEL: + r2_off += oplen2; + break; + case BAM_CSOFT_CLIP: + q2_off += oplen2; + break; + case BAM_CMATCH: + // check if match long enough and overlaps + if (r1_off - q1_off == r2_off - q2_off) // same diagonal && r1_off < r2_off + oplen2 && r2_off < r1_off + oplen1) // ref intervals intersect + { + // find the overlap size + int32_t largest_beg = TMAX (r1_off, r2_off); + int32_t smallest_end = TMIN (r1_off + oplen1, r2_off + oplen2); + int32_t overlap = smallest_end - largest_beg; + int32_t to_cover = oplen1; + if (oplen2 * MIN_OVERLAP_FACTOR >= MIN_CHECKED_MATCH_LEN && oplen2 < oplen1) + to_cover = oplen2; + if (overlap * MIN_OVERLAP_FACTOR >= to_cover) + has_overlap = 1; + } + q2_off += oplen2; + r2_off += oplen2; + break; + default: + tmap_bug (); + } + } + } + } + q1_off += oplen1; + r1_off += oplen1; + break; + default: + tmap_bug (); + } + } + if (has_long && !has_overlap) // some of long original alignment batches found to have no good overlapping match in final alignment + { + uint32_t orig_cigar_str_sz = 4 * sam->n_orig_cigar + 1; + char* orig_cigar_str = alloca (orig_cigar_str_sz); + if (!bin_cigar_to_str (sam->orig_cigar, sam->n_orig_cigar, orig_cigar_str, orig_cigar_str_sz)) + tmap_bug (); + tmap_conderr ((opt->cigar_sanity_check >= TMAP_MAP_SANITY_ERR_CONTENT_ALIGN), "Alignment sanity check error on read %s:\n post-processed alignment %s at position %d has insufficient overlap with the original one %s at position %d", seq->data.sam->name->s, cigar_str, sam->pos, orig_cigar_str, sam->orig_pos); + } + } +#endif + } - // check bounds - if(ref_start < 1) ref_start = 1; - if(refseq->annos[s->seqid].len < ref_end) { - ref_end = refseq->annos[s->seqid].len; - } - else if(ref_end < 1) ref_end = 1; - - // get the target sequence - target_len = ref_end - ref_start + 1; - if(target_mem < target_len) { - target_mem = target_len; - tmap_roundup32(target_mem); - target = tmap_realloc(target, sizeof(uint8_t)*target_mem, "target"); - } - target_len = tmap_refseq_subseq(refseq, ref_start + refseq->annos[s->seqid].offset, target_len, target); - /* - // NB: IUPAC codes are turned into mismatches - if(NULL == tmap_refseq_subseq2(refseq, sams->sams[end].seqid+1, start_pos, end_pos, target, 1, NULL)) { - tmap_bug(); - } - */ + if (opt->cigar_sanity_check == TMAP_MAP_SANITY_WARN_ALL || + opt->cigar_sanity_check == TMAP_MAP_SANITY_ERR_CONTENT_WARN_ALIGN_SCORE || + opt->cigar_sanity_check >= TMAP_MAP_SANITY_ERR_CONTENT_ALIGN_WARN_SCORE) + { + if (nogap_score < 0) + tmap_conderr ((opt->cigar_sanity_check == TMAP_MAP_SANITY_ERR_ALL), "Alignment sanity check error on read %s:\n Normal alignment score less gaps (%d) is negative (original score %d, cigar %s): possible misalignment", seq->data.sam->name->s, nogap_score, sam->result.score_fwd, cigar_str); + else if (nogap_score < (sam->result.score_fwd / 2)) + tmap_conderr ((opt->cigar_sanity_check == TMAP_MAP_SANITY_ERR_ALL), "Alignment sanity check error on read %s:\n Normal alignment score less gaps (%d) is below 1/2 of unadjusted alignment score (%d), cigar %s: possible misalignment", seq->data.sam->name->s, nogap_score, sam->result.score_fwd, cigar_str); + } +} - if(1 == s->strand) { // reverse compliment - tmap_reverse_compliment_int(target, target_len); - } - // add to the band width - param.band_width += 2 * bw; +// updates alignment box (result) from cigar, pos and target_len +// assumes cigar covers entire query, does not check with actual query +void tmap_map_update_alignment_box (tmap_map_sam_t* sam) +{ + sam->result.target_start = 0; + sam->result.target_end = sam->target_len - 1; + sam->result.query_start = 0; + uint32_t *cp, *sent, op, oplen; + for (cp = sam->cigar, sent = cp + sam->n_cigar; cp != sent; ++cp) + { + op = TMAP_SW_CIGAR_OP (*cp); + oplen = TMAP_SW_CIGAR_LENGTH (*cp); + switch (op) + { + case BAM_CMATCH: + case BAM_CDEL: + sam->result.query_end += oplen; + break; + case BAM_CSOFT_CLIP: + if (cp == sam->cigar) // first SC + sam->result.query_start = sam->result.query_end = oplen; + default: + break; + } + } +} - // make sure we have enough memory for the path - while(path_mem <= target_len + fseq->num_flows) { // lengthen the path - path_mem = target_len + fseq->num_flows + 1; - tmap_roundup32(path_mem); - path = tmap_realloc(path, sizeof(tmap_fsw_path_t)*path_mem, "path"); - } - /* - fprintf(stderr, "strand=%d\n", s->strand); - fprintf(stderr, "ref_start=%d ref_end=%d\n", ref_start, ref_end); - fprintf(stderr, "base_calls:\n"); - for(j=0;jnum_flows;j++) { - for(k=0;kbase_calls[j];k++) { - fputc("ACGTN"[fseq->flow_order[j % fseq->flow_order_len]], stderr); - } - } - fputc('\n', stderr); - fprintf(stderr, "target:\n"); - for(j=0;jflow_order_len;j++) { - fputc("ACGTN"[fseq->flow_order[j]], stderr); - } - fputc('\n', stderr); - */ - - // re-align - s->ascore = s->score; - path_len = path_mem; - //fprintf(stderr, "old score=%d\n", s->score); - switch(softclip_type) { - case TMAP_MAP_OPT_SOFT_CLIP_ALL: - s->score = tmap_fsw_clipping_core(target, target_len, fseq, ¶m, - 1, 1, s->strand, path, &path_len); - break; - case TMAP_MAP_OPT_SOFT_CLIP_LEFT: - s->score = tmap_fsw_clipping_core(target, target_len, fseq, ¶m, - 1, 0, s->strand, path, &path_len); - break; - case TMAP_MAP_OPT_SOFT_CLIP_RIGHT: - s->score = tmap_fsw_clipping_core(target, target_len, fseq, ¶m, - 0, 1, s->strand, path, &path_len); - break; - case TMAP_MAP_OPT_SOFT_CLIP_NONE: - s->score = tmap_fsw_clipping_core(target, target_len, fseq, ¶m, - 0, 0, s->strand, path, &path_len); - break; - default: - tmap_error("soft clipping type was not recognized", Exit, OutOfRange); - break; - } - s->score_subo = INT32_MIN; - //fprintf(stderr, "new score=%d path_len=%d\n", s->score, path_len); +void cigar_log (const uint32_t* cigar, unsigned cigar_sz) +{ + const uint32_t* sent; + for (sent = cigar+cigar_sz; cigar != sent; ++cigar) + { + uint32_t curop = bam_cigar_op (*cigar); + uint32_t count = bam_cigar_oplen (*cigar); + char schar; + switch (curop) + { + case BAM_CHARD_CLIP: + schar = 'H'; + break; + case BAM_CSOFT_CLIP: // skip + schar = 'S'; + break; + case BAM_CMATCH: + schar = 'M'; + break; + case BAM_CEQUAL: + schar = '='; + break; + case BAM_CDIFF: + schar = '#'; + break; + case BAM_CINS: + schar = 'I'; + break; + case BAM_CDEL: + schar = 'I'; + break; + default: + schar = '?'; + } + tmap_log ("%c%d", schar, count); + } +} - if(0 < path_len) { // update +uint32_t cigar_to_batches (const uint32_t* cigar, uint32_t cigar_sz, uint32_t* x_clip, AlBatch* batches, uint32_t max_batches) +{ + uint32_t xpos = 0, ypos = 0; // x is query, y is reference + AlBatch* curb = batches; + const uint32_t* sent = cigar + cigar_sz; + for (; cigar != sent && curb - batches != max_batches; ++cigar) + { + uint32_t curop = bam_cigar_op (*cigar); + uint32_t count = bam_cigar_oplen (*cigar); + switch (curop) + { + case BAM_CHARD_CLIP: // skip + case BAM_CSOFT_CLIP: // skip + if (x_clip) *x_clip += count; + break; + case BAM_CMATCH: + case BAM_CEQUAL: + case BAM_CDIFF: + curb->xpos = xpos; + curb->ypos = ypos; + curb->len = count; + xpos += count; + ypos += count; + ++curb; + x_clip = NULL; + break; + case BAM_CINS: + xpos += count; + x_clip = NULL; + break; + case BAM_CDEL: + ypos += count; + x_clip = NULL; + break; + default: + ; + } + } + return curb - batches; +} - /* - for(j=0;jscore = (int32_t)((s->score + 99.99)/100.0); +static const char* IB = "ACGTNBDHKMRSVWYN"; - // position - s->pos = (ref_start-1); // zero-based - // NB: must be careful of leading insertions and strandedness - if(0 == s->strand) { - if(0 <= path[path_len-1].j) { - s->pos += (path[path_len-1].j); - } - } - else { - if(path[0].j < target_len) { - s->pos += target_len - path[0].j - 1; - } - } - if(refseq->len < s->pos) { - tmap_bug(); - } +void log_batches (const char* xseq, unsigned xlen, uint8_t xrev, const char* yseq, unsigned ylen, uint8_t yrev, const AlBatch *b_ptr, int b_cnt, unsigned xoff, unsigned yoff) +{ + const unsigned margin = 0, width = 120, zero_based = 1; + + unsigned slen = 0; + unsigned blen = 0; + unsigned x = b_ptr->xpos; + unsigned y = b_ptr->ypos; + unsigned xstart = x; + unsigned ystart = y; + unsigned char xc, yc; + char s[3][MAXSTRLEN]; + + assert (width < MAXSTRLEN); + + + while (b_cnt > 0) + { + xc = xseq [x]; + yc = yseq [y]; + + // special handling for (x < b_ptr->xpos && y < b_ptr->ypos) + // treating as batch with special match symbols + + if (x < b_ptr->xpos && y < b_ptr->ypos) + { + s[0][slen] = (xc>='A')?xc:IB[xc]; + s[2][slen] = (yc>='A')?yc:IB[yc]; + s[1][slen] = '#'; + x++, y++, slen++; + } + // X insert + else if (x < b_ptr->xpos) + { + s[0][slen] = (xc>='A')?xc:IB[xc]; + s[1][slen] = ' '; + s[2][slen] = '-'; + x++, slen++; + } + // Y insert + else if (y < b_ptr->ypos) + { + s[0][slen] = '-'; + s[1][slen] = ' '; + s[2][slen] = (yc>='A')?yc:IB[yc]; + y++, slen++; + } + // emit text batch + else if (blen < b_ptr->len) + { + s[0][slen] = (xc>='A')?xc:IB[xc]; + s[2][slen] = (yc>='A')?yc:IB[yc]; + s[1][slen] = (toupper (xc) == toupper (yc) || toupper (xc) == 'N' || toupper (yc) == 'N') ? '*' : ' '; + x++, y++, slen++, blen++; + } + else + blen = 0, b_cnt--, b_ptr++; + //print accumulated lines + if ((slen + NUMSTRLEN > width) || b_cnt <= 0) + { + //null terminate all strings + for (int i = 0; i < 3; i++) + s[i][slen] = 0; - // new cigar - free(s->cigar); - s->cigar = tmap_fsw_path2cigar(path, path_len, &s->n_cigar, 1); + unsigned xdisp = (xrev ? xlen - xstart - 1 : xstart) + xoff + (zero_based ? 0 : 1); + unsigned ydisp = (yrev ? ylen - ystart - 1 : ystart) + yoff + (zero_based ? 0 : 1); - // reverse the cigar - if(1 == s->strand) { - for(i=0;in_cigar>>1;i++) { - uint32_t t = s->cigar[i]; - s->cigar[i] = s->cigar[s->n_cigar-i-1]; - s->cigar[s->n_cigar-i-1] = t; - } - } + tmap_log ("\n%*s%*d %s", margin, "", NUMSTRLEN, xdisp, s[0]); + tmap_log ("\n%*s%*s %s", margin, "", NUMSTRLEN, "", s[1]); + tmap_log ("\n%*s%*d %s\n", margin, "", NUMSTRLEN, ydisp, s[2]); - int32_t skipped_start, skipped_end; - skipped_start = skipped_end = 0; - - // soft-clipping - if(0 < path[path_len-1].i) { // skipped beginning flows - // get the number of bases to clip - for(j=0;jbase_calls[j]; - } - } - if(path[0].i+1 < fseq->num_flows) { // skipped ending flows - // get the number of bases to clip - for(j=path[0].i+1;jnum_flows;j++) { - skipped_end += fseq->base_calls[j]; - } - } - if(1 == s->strand) { // swap - k = skipped_start; - skipped_start = skipped_end; - skipped_end = k; - } - if(0 < skipped_start) { // start soft clip - s->cigar = tmap_realloc(s->cigar, sizeof(uint32_t)*(1 + s->n_cigar), "s->cigar"); - for(l=s->n_cigar-1;0<=l;l--) { - s->cigar[l+1] = s->cigar[l]; - } - TMAP_SW_CIGAR_STORE(s->cigar[0], BAM_CSOFT_CLIP, skipped_start); - s->n_cigar++; - } - if(0 < skipped_end) { // end soft clip - s->cigar = tmap_realloc(s->cigar, sizeof(uint32_t)*(1 + s->n_cigar), "s->cigar"); - s->cigar[s->n_cigar] = (skipped_end << 4) | 4; - s->n_cigar++; - } - } - } - // free - free(target); - free(path); - free(flow_order); - free(key_seq); + xstart = x, ystart = y, slen = 0; + } + } +} - if(0 == was_int) { - tmap_seq_to_char(seq); - } - return 1; + +void tmap_map_log_text_align (const char* preceed, uint32_t* cigar, uint32_t n_cigar, const char* query, uint32_t query_len, uint32_t forward, const char* ref, uint32_t ref_off) +{ + const unsigned MAX_BATCHNO = 100; + AlBatch batches [MAX_BATCHNO]; + uint32_t q_clip = 0; + int bno = cigar_to_batches (cigar, n_cigar, &q_clip, batches, MAX_BATCHNO); + + uint32_t q_len_cigar, r_len_cigar; + seq_lens_from_bin_cigar (cigar, n_cigar, &q_len_cigar, &r_len_cigar); + + if (preceed) + tmap_log (preceed); + log_batches (query+q_clip, query_len - q_clip, !forward, (const char*) ref, r_len_cigar, 0, batches, bno, q_clip, ref_off); } diff --git a/Analysis/TMAP/src/map/util/tmap_map_util.h b/Analysis/TMAP/src/map/util/tmap_map_util.h index da61be7c..c0371538 100644 --- a/Analysis/TMAP/src/map/util/tmap_map_util.h +++ b/Analysis/TMAP/src/map/util/tmap_map_util.h @@ -4,9 +4,12 @@ #include #include "../../util/tmap_rand.h" +#include "../../sw/tmap_sw.h" #include "../../sw/tmap_fsw.h" #include "../../sw/tmap_vsw.h" #include "tmap_map_opt.h" +#include "tmap_map_stats.h" + #define __map_util_gen_ap(par, opt) do { \ int32_t i; \ @@ -90,8 +93,14 @@ typedef struct { int32_t score_subo; /*!< the alignment score of the sub-optimal hit */ int32_t n_cigar; /*!< the number of cigar operators */ uint32_t *cigar; /*!< the cigar operator array */ + int32_t n_orig_cigar; + uint32_t *orig_cigar; + uint32_t orig_pos; uint16_t target_len; /*!< internal variable, the target length estimated by the seeding step */ uint16_t n_seeds; /*!< the number seeds in this hit */ + uint16_t fivep_offset; /*!< number of additional ref bases aligned if 5' not softclipped */ + uint32_t mapper_pos; + uint32_t mapper_tlen; union { tmap_map_map1_aux_t *map1_aux; /*!< auxiliary data for map1 */ tmap_map_map2_aux_t *map2_aux; /*!< auxiliary data for map2 */ @@ -127,7 +136,7 @@ typedef struct { bam1_t **bams; /*!< the bam records */ int32_t n; /*!< the number of hits */ } tmap_map_bam_t; - + /*! The multi-end BAM record structure */ @@ -136,6 +145,33 @@ typedef struct { int32_t n; /*!< the number of records (multi-end) */ } tmap_map_bams_t; +/*! + Control structure for reference sequence buffer + */ +typedef struct { + uint8_t* buf; /*!< pointer to the address of the memory buffer for unpacked reference sequence */ + uint32_t buf_sz; /*!< pointer to the variable contining presently allocated size of target_buf */ + uint8_t* data; /*!< pointer into buffer where actual requested fragment starts */ + uint32_t data_len; /*!< length of last requested data */ + uint32_t seqid; /*!< sequence Id for the fragment stored in data member, 1-based */ + uint32_t seq_start; /*!< offset of the first base of fragment stored in buf, 1-based */ + uint32_t seq_end; /*!< offset of the last base stored in buf, 1-based */ +} ref_buf_t; + +/*! + populates the ref_buf_t so that the data + @param dest (ref_buf_t*) the reference cache control structure + @param refseq (tmap_refseq_t *) pointer to the the reference server control structure + @param seqid (uint32_t) sequence id to cache, 1-based, + @param seq_start (uint32_t) position of first base in a fragment to cache, 1-based + @param seq_end (uint32_t) poition of last base of a fragment to cache, 1-based + */ + +void target_cache_init (ref_buf_t* target); +void target_cache_free (ref_buf_t* target); +void cache_target (ref_buf_t* target, tmap_refseq_t *refseq, uint32_t seqid, uint32_t seq_start, uint32_t seq_end); + + /*! initializes @param s the mapping structure @@ -369,7 +405,8 @@ tmap_map_util_mapq(tmap_map_sams_t *sams, int32_t seq_len, tmap_map_opt_t *opt, @param num_after_grouping used to return the number seeds after grouping @return the locally aligned sams */ -tmap_map_sams_t * + +tmap_map_sams_t* tmap_map_util_sw_gen_score(tmap_refseq_t *refseq, tmap_seq_t *seq, tmap_map_sams_t *sams, @@ -378,6 +415,84 @@ tmap_map_util_sw_gen_score(tmap_refseq_t *refseq, tmap_map_opt_t *opt, int32_t *num_after_grouping); +void tmap_map_util_populate_sw_par ( + tmap_sw_param_t* par, + tmap_map_opt_t* opt +); + +/*! + find alignment starts for raw mappings + @details generates the cigar after tmap_map_util_sw_gen_score has been called + @param refseq the reference sequence + @param sams the seeded sams + @param seq the original query sequence + @param seqs the query sequence (forward, reverse compliment, reverse, and compliment) + @param opt the program parameters + @param target reference data + @param stat statistics + @return the locally aligned sams + */ + +tmap_map_sams_t* +tmap_map_util_find_align_starts ( + tmap_refseq_t *refseq, // reference server + tmap_map_sams_t *sams, // initial rough mapping + tmap_seq_t *seq, // read + tmap_seq_t **seqs, // array of size 4 that contains pre-computed inverse / complement combinations + tmap_map_opt_t *opt, // tmap parameters + ref_buf_t* target, // reference data + tmap_map_stats_t *stat // statistics +); + +void tmap_map_util_align ( + tmap_refseq_t *refseq, // reference server + tmap_map_sams_t *sams, // mappings to compute alignments for + tmap_seq_t **seqs, // array of size 4 that contains pre-computed inverse / complement combinations + ref_buf_t* target, // reference data + tmap_sw_path_t** path_buf, // buffer for traceback path + int32_t* path_buf_sz, // used portion and allocated size of traceback path. + tmap_sw_param_t* par, // Smith-Waterman scopring parameters + tmap_map_stats_t *stat // statistics +); + +void tmap_map_util_salvage_edge_indels ( + tmap_refseq_t *refseq, // reference server + tmap_map_sams_t *sams, // mappings to compute alignments for + tmap_seq_t **seqs, // array of size 4 that contains pre-computed inverse / complement combinations + tmap_map_opt_t *opt, // tmap parameters + tmap_sw_param_t* par, // Smith-Waterman scopring parameters + ref_buf_t* target, // reference data cache + tmap_sw_path_t** path_buf, // buffer for traceback path + int32_t* path_buf_sz, // used portion and allocated size of traceback path. + tmap_map_stats_t *stat // statistics +); + +void tmap_map_util_cure_softclips ( + tmap_map_sams_t *sams, // mappings to compute alignments for + tmap_seq_t **seqs // array of size 4 that contains pre-computed inverse / complement combinations +); + +void tmap_map_util_trim_key ( + tmap_map_sams_t *sams, // mappings to compute alignments for + tmap_seq_t *seq, // read + tmap_seq_t **seqs, // array of size 4 that contains pre-computed inverse / complement combinations + tmap_refseq_t *refseq, // reference server + ref_buf_t* target, // reference data cache + tmap_map_stats_t *stat // statistics +); + +void tmap_map_util_end_repair_bulk ( + tmap_refseq_t *refseq, // reference server + tmap_map_sams_t *sams, // mappings to compute alignments for + tmap_seq_t *seq, // read + tmap_seq_t **seqs, // array of size 4 that contains pre-computed inverse / complement combinations + tmap_map_opt_t *opt, // tmap parameters + ref_buf_t* target, // reference data cache + tmap_sw_path_t** path_buf, // buffer for traceback path + int32_t* path_buf_sz, // used portion and allocated size of traceback path. + tmap_map_stats_t *stat // statistics +); + /*! perform local alignment @details generates the cigar after tmap_map_util_sw_gen_score has been called @@ -386,14 +501,18 @@ tmap_map_util_sw_gen_score(tmap_refseq_t *refseq, @param seq the original query sequence @param seqs the query sequence (forward, reverse compliment, reverse, and compliment) @param opt the program parameters + @param stat statistics @return the locally aligned sams */ tmap_map_sams_t * -tmap_map_util_sw_gen_cigar(tmap_refseq_t *refseq, - tmap_map_sams_t *sams, - tmap_seq_t *seq, - tmap_seq_t **seqs, - tmap_map_opt_t *opt); +tmap_map_util_sw_gen_cigar ( + tmap_refseq_t *refseq, + tmap_map_sams_t *sams, + tmap_seq_t *seq, + tmap_seq_t **seqs, + tmap_map_opt_t *opt, + tmap_map_stats_t *stat +); /*! re-aligns mappings in flow space @@ -409,11 +528,80 @@ tmap_map_util_sw_gen_cigar(tmap_refseq_t *refseq, @param pen_gape the gap extension penalty @param fscore the flow penalty @param use_flowgram 1 to use the flowgram if available, 0 otherwise + @param stat tmap statistics @return 1 if successful, 0 otherwise */ int32_t -tmap_map_util_fsw(tmap_seq_t *seq, tmap_map_sams_t *sams, tmap_refseq_t *refseq, +tmap_map_util_fsw (tmap_seq_t *seq, tmap_map_sams_t *sams, tmap_refseq_t *refseq, int32_t bw, int32_t softclip_type, int32_t score_thr, int32_t score_match, int32_t pen_mm, int32_t pen_gapo, - int32_t pen_gape, int32_t fscore, int32_t use_flowgram); + int32_t pen_gape, int32_t fscore, int32_t use_flowgram, tmap_map_stats_t* stat); + + +// updates alignment box (result) from cigar, pos and target_len +void tmap_map_update_alignment_box (tmap_map_sam_t* sam); + + +/*! + turns softclip on 5' into non-clipped alignment + @param refseq the reference sequence + @param sams the mappings to adjust + @param seq the read + @param seqs the 'alternate forms' of query sequences (forward, reverse compliment, reverse, and compliment) + @param target reference data + @param path_buf buffer for traceback path + @param path_buf_sz used portion and allocated size of traceback path. + @param par Smith-Waterman scoring parameters + @param opt the program parameters + @param stat tmap statistics + @return 1 if 5-prime was found and replaced, 0 if there was no 5' softclip + */ + +int32_t +tmap_map_util_remove_5_prime_softclip +( + tmap_refseq_t *refseq, // reference server + tmap_map_sam_t *dest_sam, // mapping to fix + tmap_seq_t *seq, // read + tmap_seq_t **seqs, // array of size 4 that contains pre-computed inverse / complement combinations + ref_buf_t* target, // reference data cache + tmap_sw_path_t** path_buf, // buffer for traceback path + int32_t* path_buf_sz, // used portion and allocated size of traceback path. + tmap_sw_param_t* par, // Smith-Waterman scoring parameters + tmap_map_opt_t *opt, // tmap options (for this stage) + tmap_map_stats_t *stat // statistics +); + +void +cigar_sanity_check +( + tmap_refseq_t *refseq, // reference server + tmap_map_sam_t *dest_sam, // mapping to fix + tmap_seq_t *seq, // read + tmap_seq_t **seqs, // array of size 4 that contains pre-computed inverse / complement combinations + ref_buf_t* target, // reference data cache + tmap_map_opt_t *opt // tmap options (for this stage) +); + +typedef struct +{ + unsigned xpos; + unsigned ypos; + unsigned len; +} +AlBatch; + +void cigar_log (const uint32_t* cigar, unsigned cigar_sz); +uint32_t cigar_to_batches (const uint32_t* cigar, uint32_t cigar_sz, uint32_t* x_clip, AlBatch* batches, uint32_t max_batches); +void log_batches (const char* xseq, unsigned xlen, uint8_t xrev, const char* yseq, unsigned ylen, uint8_t yrev, const AlBatch *b_ptr, int b_cnt, unsigned xoff, unsigned yoff); +void tmap_map_log_text_align ( + const char* preceed, + uint32_t* cigar, + uint32_t n_cigar, + const char* query, + uint32_t query_len, + uint32_t forward, + const char* ref, + uint32_t ref_off); + #endif // TMAP_MAP_UTIL_H diff --git a/Analysis/TMAP/src/realign/Realign.cpp b/Analysis/TMAP/src/realign/Realign.cpp index 1ea726d1..334cdd91 100644 --- a/Analysis/TMAP/src/realign/Realign.cpp +++ b/Analysis/TMAP/src/realign/Realign.cpp @@ -323,7 +323,7 @@ bool Realigner::computeSWalignment(vector& CigarData, vector unsigned int& start_pos_update) { // string dummy_string; - + // Compute boundaries for tubed alignment around previously found alignment if (!ComputeTubedAlignmentBoundaries()) return false; @@ -492,6 +492,8 @@ void Realigner::backtrackAlignment(unsigned int t_idx, unsigned int q_idx, int next_move = FROM_NOWHERE; int last_move = -1; + // DVK: softclip first / last deletes; + while (current_move != FROM_NOWHERE) { switch (current_move) { diff --git a/Analysis/TMAP/src/realign/cigar_op.h b/Analysis/TMAP/src/realign/cigar_op.h index 016ca1ac..be11dc52 100644 --- a/Analysis/TMAP/src/realign/cigar_op.h +++ b/Analysis/TMAP/src/realign/cigar_op.h @@ -2,17 +2,20 @@ #ifndef CIGAR_OP_H #define CIGAR_OP_H -struct CigarOp { - +struct CigarOp +{ + char Type; //!< CIGAR operation type (MIDNSHPX=) unsigned Length; //!< CIGAR operation length (number of bases) - + //! constructor - CigarOp(const char type = '\0', + CigarOp (const char type = '\0', const unsigned& length = 0) - : Type(type) - , Length(length) - { } + : + Type(type), + Length(length) + { + } }; diff --git a/Analysis/TMAP/src/realign/cigar_utils.cpp b/Analysis/TMAP/src/realign/cigar_utils.cpp index 4c78024e..7ecf453a 100644 --- a/Analysis/TMAP/src/realign/cigar_utils.cpp +++ b/Analysis/TMAP/src/realign/cigar_utils.cpp @@ -5,6 +5,7 @@ extern "C" { #include "../samtools/bam.h" #include "../util/tmap_error.h" +#include "../io/tmap_file.h" } #include @@ -56,7 +57,7 @@ const char* clip_seq (const char* qry, const uint32_t* cigar, unsigned cigar_sz, return qry + noclip_beg; } -unsigned roll_cigar (uint32_t* cigar, unsigned max_cigar_len, unsigned& cigar_len, const BATCH* batches, unsigned bno, unsigned clean_qry_len, EndClips& clip_store, unsigned& x_off, unsigned& y_off) +unsigned roll_cigar (uint32_t* cigar, unsigned max_cigar_len, unsigned& cigar_len, const BATCH* batches, unsigned bno, unsigned clean_qry_len, EndClips& clip_store, unsigned& x_off, unsigned& y_off, unsigned& xlen, unsigned& ylen) { // add beginning clips if any // x is 1st is query, y is 2nd is reference @@ -114,10 +115,16 @@ unsigned roll_cigar (uint32_t* cigar, unsigned max_cigar_len, unsigned& cigar_le } if (cur_x > clean_qry_len) { - std::cerr << "Aligned query portion extends over clipped query length\n"; + tmap_file_fprintf (tmap_file_stderr, "Aligned query portion extends over clipped query length\n"); tmap_bug (); } + xlen = cur_x - x_off; + ylen = cur_y - y_off; + + // shift x_off by the 5' soft clip + x_off += clip_store.soft_beg_; + // add ending clips if any if (clip_store.soft_end_ + (clean_qry_len - cur_x)) { diff --git a/Analysis/TMAP/src/realign/cigar_utils.h b/Analysis/TMAP/src/realign/cigar_utils.h index b5bf219b..3aa62a7b 100644 --- a/Analysis/TMAP/src/realign/cigar_utils.h +++ b/Analysis/TMAP/src/realign/cigar_utils.h @@ -38,7 +38,7 @@ const char* clip_seq (const char* qry, const uint32_t* cigar, unsigned cigar_sz, // starting insertion is added to soft clip // starting deletion is subtracted from starting insertion and result is returned as reference shift -unsigned roll_cigar (uint32_t* cigar, unsigned max_cigar_len, unsigned& cigar_len, const BATCH* batches, unsigned bno, unsigned clean_len, EndClips& clip_store, unsigned& x_off, unsigned& y_off); +unsigned roll_cigar (uint32_t* cigar, unsigned max_cigar_len, unsigned& cigar_len, const BATCH* batches, unsigned bno, unsigned clean_len, EndClips& clip_store, unsigned& x_off, unsigned& y_off, unsigned& xlen, unsigned& ylen); // conversion from cigar to batch format unsigned cigar_to_batches (const uint32_t* cigar, unsigned cigar_sz, BATCH* batches, unsigned max_batches); diff --git a/Analysis/TMAP/src/realign/contalign.cpp b/Analysis/TMAP/src/realign/contalign.cpp index 876f775c..f987d5a3 100644 --- a/Analysis/TMAP/src/realign/contalign.cpp +++ b/Analysis/TMAP/src/realign/contalign.cpp @@ -191,6 +191,7 @@ ap (NULL), xhomo (NULL), yhomo (NULL), logbuf_ (NULL), +own_log_ (NULL), logp_ (NULL) { } @@ -202,6 +203,8 @@ btrmx (NULL), ap (NULL), xhomo (NULL), yhomo (NULL), +logbuf_ (NULL), +own_log_ (NULL), logp_ (NULL) { init (max_ylen, max_xlen, max_size, gip, gep, mat, mis); @@ -413,7 +416,7 @@ double ContAlign::align_band (const char* xseq, int xlen, const char* yseq, int int y_start, y_end, y_len; //, y; char *bp_start; - double cur_w; + // double cur_w; bool bottom_in = false; double add_score; @@ -445,11 +448,12 @@ double ContAlign::align_band (const char* xseq, int xlen, const char* yseq, int if (len <= 0 || xpos + 1 == xlen || std::max (yref, ypos+1) >= std::min (ylen, ypos + 1 + bstep)) last_col = true; - cur_w = align_y_loop (ap + y_start, xseq [xpos], bp_start, xpos, xhomo [xpos - xref + 1], y_start, y_len, bottom_in, add_score, last_col); + align_y_loop (ap + y_start, xseq [xpos], bp_start, xpos, xhomo [xpos - xref + 1], y_start, y_len, bottom_in, add_score, last_col); // record max score at the top of those columns that end at the band edge - if (y_end == ylen && last_w <= cur_w) - last_w = cur_w, last_bp = bp_start + y_len - 1, last_x = xpos, last_y = y_end-1, last_reached = true; + // 02/28/2017: with the change of semantic of firstx / lastx, the lastx means alignment should terminate at the edge of X sequence. The code below terminates at Y edge => commented + // if (y_end == ylen && last_w <= cur_w) + // last_w = cur_w, last_bp = bp_start + y_len - 1, last_x = xpos, last_y = y_end-1, last_reached = true; xpos++, ypos++, bp += bstep; } @@ -578,6 +582,8 @@ unsigned ContAlign::backtrace (BATCH *b_ptr, int max_cnt, unsigned width) ++b_ptr; } // in to_first mode, if y is not fully covered, add pseudo segment and a gap + // 02/28/2017: seem to be not needed as only X coverage is now required for to_first + /* if (to_first && y >= yref) { assert (b_cnt < max_cnt); @@ -586,6 +592,7 @@ unsigned ContAlign::backtrace (BATCH *b_ptr, int max_cnt, unsigned width) b_ptr->len = 0; ++b_cnt; } + */ reverse_inplace (b_start, b_cnt); return b_cnt; } diff --git a/Analysis/TMAP/src/realign/contalign.h b/Analysis/TMAP/src/realign/contalign.h index b5c779d5..e736baee 100644 --- a/Analysis/TMAP/src/realign/contalign.h +++ b/Analysis/TMAP/src/realign/contalign.h @@ -83,6 +83,7 @@ class ContAlign // on a diagonal band (len, diag-width:diag+width_right) // returns maximum local alignment score // NOTE: batch xpos, ypos, len should be inside X and Y sequences, width > 0 + // 02/28/2017 : Changing semantics of tobeg / toend to only apply to X sequence (read in TMAP) double align_band (const char* xseq, int xlen, const char* yseq, int ylen, int xpos, int ypos, int len, int width, int width_right = -1, bool tobeg = false, bool toend = false); // checks if enough matrix space is present for alignment diff --git a/Analysis/TMAP/src/realign/realign_proxy.h b/Analysis/TMAP/src/realign/realign_proxy.h index 7f4574c8..a1a30628 100644 --- a/Analysis/TMAP/src/realign/realign_proxy.h +++ b/Analysis/TMAP/src/realign/realign_proxy.h @@ -37,7 +37,10 @@ class RealignProxy unsigned cigar_sz, uint32_t*& cigar_dest, unsigned& cigar_dest_sz, - int& new_r_pos, + unsigned& new_r_pos, + unsigned& new_q_pos, + unsigned& new_r_len, + unsigned& new_q_len, bool& already_perfect, bool& clip_failed, bool& alignment_failed, diff --git a/Analysis/TMAP/src/realign/realign_wrapper.cpp b/Analysis/TMAP/src/realign/realign_wrapper.cpp index 058ae70a..1d54763b 100644 --- a/Analysis/TMAP/src/realign/realign_wrapper.cpp +++ b/Analysis/TMAP/src/realign/realign_wrapper.cpp @@ -79,7 +79,10 @@ uint8_t realigner_compute_alignment (RealignProxy* r, unsigned cigar_sz, uint32_t** cigar_dest, unsigned* cigar_dest_sz, - int* new_r_pos, + unsigned* new_r_pos, + unsigned* new_q_pos, + unsigned* new_r_len, + unsigned* new_q_len, uint64_t* num_realign_already_perfect, uint64_t* num_realign_not_clipped, uint64_t* num_realign_sw_failures, @@ -90,7 +93,7 @@ uint8_t realigner_compute_alignment (RealignProxy* r, bool alignment_failed = false; bool unclip_failed = false; - bool result = r->compute_alignment (q_seq, q_len, r_seq, r_len, r_pos, (bool) forward, cigar, cigar_sz, *cigar_dest, *cigar_dest_sz, *new_r_pos, already_perfect, clip_failed, alignment_failed, unclip_failed); + bool result = r->compute_alignment (q_seq, q_len, r_seq, r_len, r_pos, (bool) forward, cigar, cigar_sz, *cigar_dest, *cigar_dest_sz, *new_r_pos, *new_q_pos, *new_r_len, *new_q_len, already_perfect, clip_failed, alignment_failed, unclip_failed); if (num_realign_already_perfect && already_perfect) ++(*num_realign_already_perfect); if (num_realign_not_clipped && clip_failed) ++(*num_realign_not_clipped); diff --git a/Analysis/TMAP/src/realign/realign_wrapper.h b/Analysis/TMAP/src/realign/realign_wrapper.h index df793dfb..22723877 100644 --- a/Analysis/TMAP/src/realign/realign_wrapper.h +++ b/Analysis/TMAP/src/realign/realign_wrapper.h @@ -45,7 +45,10 @@ uint8_t realigner_compute_alignment ( unsigned cigar_sz, uint32_t** cigar_dest, unsigned* cigar_dest_sz, - int* new_r_pos, + unsigned* new_r_pos, + unsigned* new_q_pos, + unsigned* new_r_len, + unsigned* new_q_len, uint64_t* num_realign_already_perfect, uint64_t* num_realign_not_clipped, uint64_t* num_realign_sw_failures, diff --git a/Analysis/TMAP/src/realign/realign_wrapper_context_imp.cpp b/Analysis/TMAP/src/realign/realign_wrapper_context_imp.cpp index 94c00099..cd65e4cd 100644 --- a/Analysis/TMAP/src/realign/realign_wrapper_context_imp.cpp +++ b/Analysis/TMAP/src/realign/realign_wrapper_context_imp.cpp @@ -50,7 +50,7 @@ char* ContAlignImp::qry_buf (unsigned len) return qry_buf_; qry_buf_len_ = len_roundup (len); if (qry_buf_) - delete qry_buf_; + delete [] qry_buf_; // TODO: memory allocation error handling qry_buf_ = new char [qry_buf_len_]; return qry_buf_; @@ -64,7 +64,7 @@ char* ContAlignImp::ref_buf (unsigned len) return ref_buf_; ref_buf_len_ = len_roundup (len); if (ref_buf_) - delete ref_buf_; + delete [] ref_buf_; // TODO: memory allocation error handling ref_buf_ = new char [ref_buf_len_]; return ref_buf_; @@ -126,7 +126,10 @@ bool ContAlignImp::compute_alignment ( unsigned cigar_sz, uint32_t*& cigar_dest, unsigned& cigar_dest_sz, - int& new_pos, + unsigned& new_ref_pos, + unsigned& new_qry_pos, + unsigned& new_ref_len, + unsigned& new_qry_len, bool& already_perfect, bool& clip_failed, bool& alignment_failed, @@ -192,10 +195,10 @@ bool ContAlignImp::compute_alignment ( ref_ins + extra_bandwidth_ // width ); // convert alignment to cigar - unsigned qry_off, ref_off; + unsigned ref_off; // int ref_shift = - roll_cigar (new_cigar_, MAX_CIGAR_SZ, cigar_dest_sz, batches_, bno, clean_len, clips, qry_off, ref_off); - new_pos = r_pos + ref_off; + roll_cigar (new_cigar_, MAX_CIGAR_SZ, cigar_dest_sz, batches_, bno, clean_len, clips, new_qry_pos, ref_off, new_qry_len, new_ref_len); + new_ref_pos = r_pos + ref_off; cigar_dest = new_cigar_; return true; diff --git a/Analysis/TMAP/src/realign/realign_wrapper_context_imp.h b/Analysis/TMAP/src/realign/realign_wrapper_context_imp.h index 23d3c666..c82978fd 100644 --- a/Analysis/TMAP/src/realign/realign_wrapper_context_imp.h +++ b/Analysis/TMAP/src/realign/realign_wrapper_context_imp.h @@ -52,7 +52,10 @@ class ContAlignImp : public RealignProxy, protected ContAlign unsigned cigar_sz, uint32_t*& cigar_dest, unsigned& cigar_dest_sz, - int& new_pos, + unsigned& new_ref_pos, + unsigned& new_qry_pos, + unsigned& new_ref_len, + unsigned& new_qry_len, bool& already_perfect, bool& clip_failed, bool& alignment_failed, diff --git a/Analysis/TMAP/src/realign/realign_wrapper_imp.cpp b/Analysis/TMAP/src/realign/realign_wrapper_imp.cpp index 3c6de09e..30b1ccb9 100644 --- a/Analysis/TMAP/src/realign/realign_wrapper_imp.cpp +++ b/Analysis/TMAP/src/realign/realign_wrapper_imp.cpp @@ -128,7 +128,10 @@ bool RealignImp::compute_alignment ( unsigned cigar_sz, uint32_t*& cigar_dest, unsigned& cigar_dest_sz, - int& new_pos, + unsigned& new_ref_pos, + unsigned& new_qry_pos, + unsigned& new_ref_len, + unsigned& new_qry_len, bool& already_perfect, bool& clip_failed, bool& alignment_failed, @@ -193,13 +196,13 @@ bool RealignImp::compute_alignment ( vector new_cigar_vec; unsigned int start_pos_shift; - if (!computeSWalignment(new_cigar_vec, new_md_vec, start_pos_shift)) + if (!computeSWalignment (new_cigar_vec, new_md_vec, start_pos_shift)) { alignment_failed = true; return false; } - if (!addClippedBasesToTags(new_cigar_vec, new_md_vec, q_len)) + if (!addClippedBasesToTags (new_cigar_vec, new_md_vec, q_len)) { unclip_failed = true; return false; // error adding back clipped out zones @@ -209,12 +212,23 @@ bool RealignImp::compute_alignment ( { // build cigar data only if it is needed // TODO avoid automatic vectors to prevent unneeded heap usage - std::vector cigar_vec; + CigarVec cigar_vec; cigar_vector_from_bin (cigar, cigar_sz, cigar_vec); - new_pos = updateReadPosition (cigar_vec, start_pos_shift, r_pos); + new_ref_pos = updateReadPosition (cigar_vec, start_pos_shift, r_pos); } else - new_pos = r_pos; + new_ref_pos = r_pos; + + // here we need to adjust for the possible weirdness of Realigner: + // it may produce alignments starting / ending with indels. + // They are to be converted to softclips or to ref shifts. + // Namely: + // insertions at the start and at the end should be added to softclips + // deletions at the starts should be added to new_ref_pos + // deletions at the end should be subtracted from new_ref_len + // we do processing here in order to avoid touching code inherited from Bamrealignment (can be re-considered later) + // the + adjust_cigar (new_cigar_vec, new_ref_pos, new_ref_pos, new_qry_pos, new_ref_len, new_qry_len); // free (cigar_dest); // TODO: switch to better alignment memory management, avoid heap operations @@ -226,5 +240,101 @@ bool RealignImp::compute_alignment ( } +void adjust_cigar (CigarVec& src, unsigned start_pos_shift, unsigned& new_ref_pos, unsigned& new_qry_pos, unsigned& new_ref_len, unsigned& new_qry_len) +{ + // walk from front to first aligned pair of bases. Count softclips and Is as softclip, Ds as reference shifts + // it is safe to reset new_ref_pos since even if same var is passed as start_pos_shif and new_ref_pos, the former is copied on stack. + new_ref_pos = new_qry_pos = new_ref_len = new_qry_len = 0; + if (!src.size ()) + { + new_ref_pos = start_pos_shift; + return; + } + // walks toward the end to compute ref and lengths + bool in_prefix = true; + unsigned last_prefix_idx = 0; + for (CigarVec::iterator ci = src.begin (); ci != src.end (); ++ci) + { + switch (ci->Type) + { + case 'M': // aligned + case 'X': // mismatch + case '=': // match + // both ref and qry consumed + new_ref_len += ci->Length, new_qry_len += ci->Length; + in_prefix = false; // stops accumulating left flank + break; + case 'I': // insert + case 'S': // softclip + // qry consumed, ref not + if (in_prefix) + new_qry_pos += ci->Length, ++last_prefix_idx; + else + new_qry_len += ci->Length; + break; + case 'D': // delete + case 'N': // refskip + // ref consumed, qry not + if (in_prefix) + new_ref_pos += ci->Length, ++last_prefix_idx; + else + new_ref_len += ci->Length; + break; + default: // hardclip and pad + // none consumed + if (in_prefix) + ++last_prefix_idx; + break; + } + } + // now figure out the suffix - walk backward + bool in_suffix = true; + unsigned first_suffix_idx = src.size (); + for (CigarVec::reverse_iterator ri = src.rbegin (); in_suffix && ri != src.rend (); ++ri) + { + switch (ri->Type) + { + case 'M': // aligned + case 'X': // mismatch + case '=': // match + // both ref and qry consumed + in_suffix = false; // stops accumulating left flank + break; + case 'I': // insert + case 'S': // softclip + // qry consumed, ref not + assert (new_qry_len >= ri->Length); + assert (first_suffix_idx); + new_qry_len -= ri->Length; + --first_suffix_idx; + break; + case 'D': // delete + case 'N': // refskip + // ref consumed, qry not + assert (new_ref_len >= ri->Length); + assert (first_suffix_idx); + new_ref_len -= ri->Length; + --first_suffix_idx; + break; + default: // hardclip and pad + // none consumed + assert (first_suffix_idx); + --first_suffix_idx; + break; + } + } + assert (last_prefix_idx <= first_suffix_idx); + // take the suffix out + src.resize (first_suffix_idx); + // replace prefix with just one softclip (assuming no hard clip encountered - TMAP does not produce one + if (last_prefix_idx) + { + src.erase (src.begin (), src.begin () + (last_prefix_idx - 1)); // leave one position + src.front ().Type = 'S'; + src.front ().Length = new_qry_pos; + } + // add prior start offset + new_ref_pos += start_pos_shift; +} diff --git a/Analysis/TMAP/src/realign/realign_wrapper_imp.h b/Analysis/TMAP/src/realign/realign_wrapper_imp.h index f42e37d6..76bbbe01 100644 --- a/Analysis/TMAP/src/realign/realign_wrapper_imp.h +++ b/Analysis/TMAP/src/realign/realign_wrapper_imp.h @@ -5,8 +5,15 @@ #include "realign_proxy.h" #include "Realign.h" +typedef std::vector CigarVec; +// compensate for cigar discrepancies (in/dels at the alignment edges) +// that is possible result of realignment +// (kind of a hack, implemented in outer layer to avoid meessing with original Realign code from bamrealignment +void adjust_cigar (CigarVec& src, unsigned start_pos_shift, unsigned& new_ref_pos, unsigned& new_qry_pos, unsigned& new_ref_len, unsigned& new_qry_len); + class RealignImp : public RealignProxy, protected Realigner { +private: CLIPTYPE cliptype_; // buffers for reference and inverse query sequences @@ -21,6 +28,9 @@ class RealignImp : public RealignProxy, protected Realigner unsigned len_roundup (unsigned len); + + + protected: RealignImp (); RealignImp (unsigned reserve_size, unsigned clipping_size); @@ -50,7 +60,10 @@ class RealignImp : public RealignProxy, protected Realigner unsigned cigar_sz, uint32_t*& cigar_dest, unsigned& cigar_dest_sz, - int& new_pos, + unsigned& new_ref_pos, + unsigned& new_qry_pos, + unsigned& new_ref_len, + unsigned& new_qry_len, bool& already_perfect, bool& clip_failed, bool& alignment_failed, diff --git a/Analysis/TMAP/src/sw/tmap_fsw.c b/Analysis/TMAP/src/sw/tmap_fsw.c index 60cc3228..7170cddd 100644 --- a/Analysis/TMAP/src/sw/tmap_fsw.c +++ b/Analysis/TMAP/src/sw/tmap_fsw.c @@ -942,7 +942,7 @@ tmap_fsw_path2cigar(const tmap_fsw_path_t *path, int32_t path_len, int32_t *n_ci } *n_cigar = n; cigar = tmap_malloc(*n_cigar * 4, "cigar"); - + // get the last type dpscore_last_type = tmap_fsw_path2cigar_get_type(path[path_len-1].ctype); cigar[0] = 1u << 4 | dpscore_last_type; diff --git a/Analysis/TMAP/src/sw/tmap_sw.h b/Analysis/TMAP/src/sw/tmap_sw.h index 4a604a84..832901f2 100644 --- a/Analysis/TMAP/src/sw/tmap_sw.h +++ b/Analysis/TMAP/src/sw/tmap_sw.h @@ -110,7 +110,7 @@ typedef struct int32_t i; /*!< the seq1 index (1-based) */ int32_t j; /*!< the seq2 index (1-based) */ uint8_t ctype; /*!< the edit operator applied */ -} tmap_sw_path_t; +} tmap_sw_path_t; /*! Stores a Smith Waterman alignment. @@ -313,6 +313,7 @@ uint32_t * tmap_sw_path2cigar(const tmap_sw_path_t *path, int32_t path_len, int32_t *n_cigar); + /******************** * global variables * ********************/ diff --git a/Analysis/TMAP/src/util/tmap_definitions.c b/Analysis/TMAP/src/util/tmap_definitions.c index ab0ad06d..6d43e212 100644 --- a/Analysis/TMAP/src/util/tmap_definitions.c +++ b/Analysis/TMAP/src/util/tmap_definitions.c @@ -615,10 +615,10 @@ tmap_version(int argc, char *argv[]) #ifdef GIT_REV fprintf(stderr, "%sVersion: %s%s%s %sgit:%s%s%s\n", KRED, KWHT, PACKAGE_VERSION, KNRM, KRED, KWHT, GIT_REV, KNRM); #else - fprintf(stderr, "%sVersion: %s%s%s\n", KRED, KWHT, PACKAGE_VERSION, KNRM); + fprintf(stderr, "%sVersion: %s%s%s\n\n", KRED, KWHT, PACKAGE_VERSION, KNRM); #endif //fprintf(stderr, "%sWeb Site: %s%s%s\n", KRED, KWHT, "http://github.com/iontorrent/tmap", KNRM); - fprintf(stderr, "%sContact: %s%s%s\n\n", KRED, KWHT, PACKAGE_BUGREPORT, KNRM); + //fprintf(stderr, "%sContact: %s%s%s\n\n", KRED, KWHT, PACKAGE_BUGREPORT, KNRM); return 0; } diff --git a/Analysis/TMAP/src/util/tmap_error.c b/Analysis/TMAP/src/util/tmap_error.c index bf995d19..2ee25689 100644 --- a/Analysis/TMAP/src/util/tmap_error.c +++ b/Analysis/TMAP/src/util/tmap_error.c @@ -4,6 +4,25 @@ #include #include #include "tmap_error.h" +#include + +#ifdef HAVE_LIBPTHREAD +#include +static pthread_mutex_t err_mutex = PTHREAD_MUTEX_INITIALIZER; +#endif + +static void elock () +{ +#ifdef HAVE_LIBPTHREAD + pthread_mutex_lock (&err_mutex); +#endif +} +static void eunlock () +{ +#ifdef HAVE_LIBPTHREAD + pthread_mutex_unlock (&err_mutex); +#endif +} static char error_string[][64] = { @@ -110,3 +129,33 @@ tmap_error_full(const char *file, const unsigned int line, const char *function_ break; } } + + +void +tmap_warn (const char* src_fname, const char* src_func, int src_lno, const char *fmt, ...) +{ + elock (); + va_list ap; + va_start (ap, fmt); + fprintf (stderr, "Warning: "); + vfprintf (stderr, fmt, ap); + fprintf (stderr, "\n (In file: %s, function: %s, line %d)\n", src_fname, src_func, src_lno); + va_end (ap); + eunlock (); +} + +void +tmap_fail (int quit, const char* src_fname, const char* src_func, int src_lno, const char *fmt, ...) +{ + elock (); + va_list ap; + va_start(ap, fmt); + fprintf (stderr, "Error: "); + vfprintf (stderr, fmt, ap); + fprintf (stderr, "\n (In file: %s, function: %s, line %d)\n", src_fname, src_func, src_lno); + va_end(ap); + eunlock (); + if (quit) + exit(EXIT_FAILURE); +} + diff --git a/Analysis/TMAP/src/util/tmap_error.h b/Analysis/TMAP/src/util/tmap_error.h index b33fbba9..44812d1b 100644 --- a/Analysis/TMAP/src/util/tmap_error.h +++ b/Analysis/TMAP/src/util/tmap_error.h @@ -102,6 +102,24 @@ tmap_error_cmd_check_int64(int64_t val, int64_t lower, int64_t upper, char *opti @param error_type the error type */ void -tmap_error_full(const char *file, const unsigned int line, const char *function_name, const char *variable_name, int action_type, int error_type); +tmap_error_full (const char *file, const unsigned int line, const char *function_name, const char *variable_name, int action_type, int error_type); + + + +void +tmap_fail (int fail, const char* fname, const char* func_name, int lineno, const char *fmt, ...); + +void +tmap_warn (const char* fname, const char* func_name, int lineno, const char *fmt, ...); + + +#define tmap_conderr(quit, fmt, args...) \ + tmap_fail (quit, __FILE__, __func__, __LINE__, fmt, args) + +#define tmap_failure(fmt, args...) \ + tmap_fail (1, __FILE__, __func__, __LINE__, fmt, args) + +#define tmap_warning(fmt, args...) \ + tmap_warn (__FILE__, __func__, __LINE__, fmt, args) #endif // TMAP_ERROR_H diff --git a/Analysis/TsInputUtil/perChipJsonEditor.cpp b/Analysis/TsInputUtil/perChipJsonEditor.cpp new file mode 100644 index 00000000..2895feec --- /dev/null +++ b/Analysis/TsInputUtil/perChipJsonEditor.cpp @@ -0,0 +1,1098 @@ +/* Copyright (C) 2012 Ion Torrent Systems, Inc. All Rights Reserved */ + +#include +#include +#include +#include +#include +#include +#include +#include +#include +#include + +#include "json/json.h" + + +#define PER_CHIP_JSON_EDITOR_VERSION "1.0.0" +#define WAIT_TIME 60 + +using namespace std; +using namespace Json; + +enum OptType { + OT_BOOL = 0, + OT_INT, + OT_DOUBLE, + OT_STRING, + OT_VECTOR_INT, + OT_VECTOR_DOUBLE, + OT_UNKOWN +}; + +void saveJson(const Json::Value & json, const string& filename_json) +{ + ofstream out(filename_json.c_str(), ios::out); + if (out.good()) + { + out << json.toStyledString(); + } + else + { + cout << "tsinpututil ERROR: unable to write JSON file " << filename_json << endl; + } + + out.close(); +} + +void usageMain() +{ + cerr << "perchipjsoneditor - edit per chip json files" << endl; + cerr << "Usage: " << endl + << " perchipjsoneditor [perChipJsonDirectory]" << endl + << " operation:" << endl + << " create" << endl + << " edit" << endl + << " validate" << endl + << " diff" < mapOptType; + mapOptType["acqPrefix"] = OT_STRING; + mapOptType["always-start-slow"] = OT_BOOL; + mapOptType["analysis-region"] = OT_VECTOR_INT; + mapOptType["barcode-debug"] = OT_BOOL; + mapOptType["barcode-flag"] = OT_BOOL; + mapOptType["barcode-penalty"] = OT_DOUBLE; + mapOptType["barcode-radius"] = OT_DOUBLE; + mapOptType["barcode-spec-file"] = OT_STRING; + mapOptType["barcode-tie"] = OT_DOUBLE; + mapOptType["beadfind-basis"] = OT_STRING; + mapOptType["beadfind-bfmult"] = OT_DOUBLE; + mapOptType["beadfind-bgdat"] = OT_STRING; + mapOptType["beadfind-blob-filter"] = OT_BOOL; + mapOptType["beadfind-dat"] = OT_STRING; + mapOptType["beadfind-diagnostics"] = OT_INT; + mapOptType["beadfind-filt-noisy-col"] = OT_STRING; + mapOptType["beadfind-gain-correction"] = OT_BOOL; + mapOptType["beadfind-lib-filt"] = OT_DOUBLE; + mapOptType["beadfind-lib-min-peak"] = OT_DOUBLE; + mapOptType["beadfind-mesh-step"] = OT_VECTOR_INT; + mapOptType["beadfind-min-tf-snr"] = OT_DOUBLE; + mapOptType["beadfind-minlive"] = OT_DOUBLE; + mapOptType["beadfind-minlivesnr"] = OT_DOUBLE; + mapOptType["beadfind-num-threads"] = OT_INT; + mapOptType["beadfind-predict-end"] = OT_INT; + mapOptType["beadfind-predict-start"] = OT_INT; + mapOptType["beadfind-sdasbf"] = OT_BOOL; + mapOptType["beadfind-sep-ref"] = OT_BOOL; + mapOptType["beadfind-sig-ref-type"] = OT_INT; + mapOptType["beadfind-skip-sd-recover"] = OT_INT; + mapOptType["beadfind-smooth-trace"] = OT_BOOL; + mapOptType["beadfind-tf-filt"] = OT_DOUBLE; + mapOptType["beadfind-tf-min-peak"] = OT_DOUBLE; + mapOptType["beadfind-type"] = OT_STRING; + mapOptType["beadfind-zero-flows"] = OT_STRING; + mapOptType["beadfindfile"] = OT_STRING; + mapOptType["beadmask-categorized"] = OT_BOOL; + mapOptType["bfold"] = OT_BOOL; + mapOptType["bfonly"] = OT_BOOL; + mapOptType["bkg-ampl-lower-limit"] = OT_DOUBLE; + mapOptType["bkg-bfmask-update"] = OT_BOOL; + mapOptType["bkg-copy-stringency"] = OT_DOUBLE; + mapOptType["bkg-dbg-trace"] = OT_VECTOR_INT; + mapOptType["bkg-debug-files"] = OT_BOOL; + mapOptType["bkg-debug-nsamples"] = OT_INT; + mapOptType["bkg-debug-param"] = OT_INT; + mapOptType["bkg-debug-region"] = OT_VECTOR_INT; + mapOptType["bkg-debug-trace-rcflow"] = OT_STRING; + mapOptType["bkg-debug-trace-sse"] = OT_STRING; + mapOptType["bkg-debug-trace-xyflow"] = OT_STRING; + mapOptType["bkg-dont-emphasize-by-compression"] = OT_BOOL; + mapOptType["bkg-empty-well-normalization"] = OT_BOOL; + mapOptType["bkg-exp-tail-bkg-adj"] = OT_BOOL; + mapOptType["bkg-exp-tail-bkg-limit"] = OT_DOUBLE; + mapOptType["bkg-exp-tail-bkg-lower"] = OT_DOUBLE; + mapOptType["bkg-exp-tail-fit"] = OT_BOOL; + mapOptType["bkg-exp-tail-tau-adj"] = OT_BOOL; + mapOptType["bkg-kmult-adj-low-hi"] = OT_DOUBLE; + mapOptType["bkg-max-rank-beads"] = OT_INT; + mapOptType["bkg-min-sampled-beads"] = OT_INT; + mapOptType["bkg-pca-dark-matter"] = OT_BOOL; + mapOptType["bkg-per-flow-time-tracking"] = OT_BOOL; + mapOptType["bkg-post-key-step"] = OT_INT; + mapOptType["bkg-post-key-train"] = OT_INT; + mapOptType["bkg-prefilter-beads"] = OT_BOOL; + mapOptType["bkg-recompress-tail-raw-trace"] = OT_BOOL; + mapOptType["bkg-single-gauss-newton"] = OT_BOOL; + mapOptType["bkg-use-duds"] = OT_BOOL; + mapOptType["bkg-use-proton-well-correction"] = OT_BOOL; + mapOptType["bkg-washout-flow-detection"] = OT_INT; + mapOptType["bkg-washout-threshold"] = OT_DOUBLE; + mapOptType["bkg-well-xtalk-name"] = OT_STRING; + mapOptType["clonal-filter-bkgmodel"] = OT_BOOL; + mapOptType["clonal-filter-debug"] = OT_BOOL; + mapOptType["clonal-filter-use-last-iter-params"] = OT_BOOL; + mapOptType["col-doubles-xtalk-correct"] = OT_BOOL; + mapOptType["col-flicker-correct"] = OT_BOOL; + mapOptType["col-flicker-correct-aggressive"] = OT_BOOL; + mapOptType["col-flicker-correct-verbose"] = OT_BOOL; + mapOptType["corr-noise-correct"] = OT_BOOL; + mapOptType["cropped"] = OT_VECTOR_INT; + mapOptType["cropped-region-origin"] = OT_VECTOR_INT; + mapOptType["dark-matter-correction"] = OT_BOOL; + mapOptType["dat-postfix"] = OT_STRING; + mapOptType["dat-source-directory"] = OT_STRING; + mapOptType["datacollect-gain-correction"] = OT_BOOL; + mapOptType["debug-bead-only"] = OT_BOOL; + mapOptType["double-tap-means-zero"] = OT_BOOL; + mapOptType["explog-path"] = OT_STRING; + mapOptType["filter-extreme-ppf-only"] = OT_BOOL; + mapOptType["fit-region-kmult"] = OT_BOOL; + mapOptType["fitting-taue"] = OT_BOOL; + mapOptType["flow-order"] = OT_STRING; + mapOptType["flowlimit"] = OT_INT; + mapOptType["flowtimeoffset"] = OT_INT; + mapOptType["fluid-potential-correct"] = OT_BOOL; + mapOptType["fluid-potential-threshold"] = OT_DOUBLE; + mapOptType["forcenn"] = OT_INT; + mapOptType["frames"] = OT_INT; + mapOptType["from-beadfind"] = OT_BOOL; + mapOptType["gopt"] = OT_STRING; + mapOptType["gpu-amp-guess"] = OT_INT; + mapOptType["gpu-device-ids"] = OT_INT; + mapOptType["gpu-fitting-only"] = OT_BOOL; + mapOptType["gpu-flow-by-flow"] = OT_BOOL; + mapOptType["gpu-force-multi-flow-fit"] = OT_BOOL; + mapOptType["gpu-hybrid-fit-iter"] = OT_INT; + mapOptType["gpu-memory-per-proc"] = OT_INT; + mapOptType["gpu-multi-flow-fit"] = OT_INT; + mapOptType["gpu-multi-flow-fit-blocksize"] = OT_INT; + mapOptType["gpu-multi-flow-fit-l1config"] = OT_INT; + mapOptType["gpu-num-history-flows"] = OT_INT; + mapOptType["gpu-num-streams"] = OT_INT; + mapOptType["gpu-partial-deriv-blocksize"] = OT_INT; + mapOptType["gpu-partial-deriv-l1config"] = OT_INT; + mapOptType["gpu-single-flow-fit"] = OT_INT; + mapOptType["gpu-single-flow-fit-blocksize"] = OT_INT; + mapOptType["gpu-single-flow-fit-l1config"] = OT_INT; + mapOptType["gpu-single-flow-fit-type"] = OT_INT; + mapOptType["gpu-switch-to-flow-by-flow-at"] = OT_INT; + mapOptType["gpu-use-all-devices"] = OT_BOOL; + mapOptType["gpu-verbose"] = OT_BOOL; + mapOptType["gpuworkload"] = OT_DOUBLE; + mapOptType["hilowfilter"] = OT_BOOL; + mapOptType["ignore-checksum-errors"] = OT_BOOL; + mapOptType["ignore-checksum-errors-1frame"] = OT_BOOL; + mapOptType["img-gain-correct"] = OT_BOOL; + mapOptType["incorporation-type"] = OT_INT; + mapOptType["kmult-hi-limit"] = OT_DOUBLE; + mapOptType["kmult-low-limit"] = OT_DOUBLE; + mapOptType["kmult-penalty"] = OT_DOUBLE; + mapOptType["librarykey"] = OT_STRING; + mapOptType["limit-rdr-fit"] = OT_BOOL; + mapOptType["local-wells-file"] = OT_BOOL; + mapOptType["mask-datacollect-exclude-regions"] = OT_BOOL; + mapOptType["max-iterations"] = OT_INT; + mapOptType["mixed-first-flow"] = OT_INT; + mapOptType["mixed-last-flow"] = OT_INT; + mapOptType["mixed-model-option"] = OT_INT; + mapOptType["mixed-stringency"] = OT_DOUBLE; + mapOptType["n-unfiltered-lib"] = OT_INT; + mapOptType["nn-subtract-empties"] = OT_BOOL; + mapOptType["nnmask"] = OT_VECTOR_INT; + mapOptType["nnmaskwh"] = OT_VECTOR_INT; + mapOptType["no-subdir"] = OT_BOOL; + mapOptType["no-threaded-file-access"] = OT_BOOL; + mapOptType["noduds"] = OT_BOOL; + mapOptType["nokey"] = OT_BOOL; + mapOptType["nuc-correct"] = OT_INT; + mapOptType["num-regional-samples"] = OT_INT; + mapOptType["numcputhreads"] = OT_INT; + mapOptType["output-dir"] = OT_STRING; + mapOptType["output-pinned-wells"] = OT_BOOL; + mapOptType["pair-xtalk-coeff"] = OT_DOUBLE; + mapOptType["pass-tau"] = OT_BOOL; + mapOptType["pca-test"] = OT_STRING; + mapOptType["post-fit-handshake-worker"] = OT_BOOL; + mapOptType["readaheaddat"] = OT_INT; + mapOptType["region-list"] = OT_VECTOR_INT; + mapOptType["region-size"] = OT_VECTOR_INT; + mapOptType["region-vfrc-debug"] = OT_BOOL; + mapOptType["regional-sampling"] = OT_BOOL; + mapOptType["regional-sampling-type"] = OT_INT; + mapOptType["restart-check"] = OT_BOOL; + mapOptType["restart-from"] = OT_STRING; + mapOptType["restart-next"] = OT_STRING; + mapOptType["restart-region-params-file"] = OT_STRING; + mapOptType["revert-regional-sampling"] = OT_BOOL; + mapOptType["sigproc-compute-flow"] = OT_STRING; + mapOptType["sigproc-regional-smoothing-alpha"] = OT_DOUBLE; + mapOptType["sigproc-regional-smoothing-gamma"] = OT_DOUBLE; + mapOptType["skip-first-flow-block-regional-fitting"] = OT_BOOL; + mapOptType["smoothing"] = OT_STRING; + mapOptType["smoothing-file"] = OT_STRING; + mapOptType["stack-dump-file"] = OT_STRING; + mapOptType["start-flow-plus-interval"] = OT_VECTOR_INT; + mapOptType["stop-beads"] = OT_BOOL; + mapOptType["suppress-copydrift"] = OT_BOOL; + mapOptType["tfkey"] = OT_STRING; + mapOptType["total-timeout"] = OT_INT; + mapOptType["trim-ref-trace"] = OT_STRING; + mapOptType["use-alternative-etbr-equation"] = OT_BOOL; + mapOptType["use-beadmask"] = OT_STRING; + mapOptType["exclusion-mask"] = OT_STRING; + mapOptType["use-pinned"] = OT_BOOL; + mapOptType["use-safe-buffer-model"] = OT_BOOL; + mapOptType["vectorize"] = OT_BOOL; + mapOptType["well-stat-file"] = OT_STRING; + mapOptType["wells-compression"] = OT_INT; + mapOptType["wells-convert-high"] = OT_DOUBLE; + mapOptType["wells-convert-low"] = OT_DOUBLE; + mapOptType["wells-convert-with-copies"] = OT_BOOL; + mapOptType["wells-format"] = OT_STRING; + mapOptType["wells-save-as-ushort"] = OT_BOOL; + mapOptType["wells-save-flow"] = OT_INT; + mapOptType["wells-save-freq"] = OT_INT; + mapOptType["wells-save-number-copies"] = OT_BOOL; + mapOptType["wells-save-queue-size"] = OT_INT; + mapOptType["xtalk"] = OT_STRING; + mapOptType["xtalk-correction"] = OT_BOOL; + + if(argc == 2) + { + string option = argv[1]; + if("-h" == option) + { + usageMain(); + } + else if("-v" == option) + { + cerr << "perchipjsoneditor version: " << PER_CHIP_JSON_EDITOR_VERSION << endl; + usageMain(); + } + } + + string jsonDir("../config"); + if(argc > 1) + { + jsonDir = argv[1]; + } + + struct stat sb; + if(!(stat(jsonDir.c_str(), &sb) == 0 && S_ISDIR(sb.st_mode))) + { + cerr << "ERROR: " << jsonDir << " does not exist or it is not a directory. Please provide the correct directory where per chip json files locate." << endl; + cout << "Please provide correct per chip json file directory (or type q to quit): "; + cin >> jsonDir; + + if(jsonDir == "q") + { + cout << "Bye." << endl; + exit(0); + } + + if(!(stat(jsonDir.c_str(), &sb) == 0 && S_ISDIR(sb.st_mode))) + { + cerr << "ERROR: " << jsonDir << " does not exist or it is not a directory. Please re-run the program with correct directory where per chip json files locate." << endl; + exit ( EXIT_FAILURE ); + } + } + + vector vecChipType; + map mapChipType; + + string cmdline("ls "); + cmdline += jsonDir; + cmdline += "/args_*.json > ./tmpFileList.txt"; + int ret = system(cmdline.c_str()); + if(ret != 0) + { + cerr << "ERROR: There is no per chip json file at " << jsonDir << endl; + exit ( EXIT_FAILURE ); + } + + char buf[2048]; + ifstream ifs("./tmpFileList.txt"); + while(ifs.getline(buf, 2048)) + { + string chipType = buf; + int index = chipType.rfind("/"); + if(index >= 0) + { + chipType = chipType.substr(index + 1, chipType.length() - index - 1); + } + + chipType = chipType.substr(5, chipType.length() - 5); + index = chipType.find("_"); + if(index > 0) + { + chipType = chipType.substr(0, index); + } + + map::iterator iter = mapChipType.find(chipType); + if(iter == mapChipType.end()) + { + mapChipType[chipType] = 1; + vecChipType.push_back(chipType); + } + else + { + mapChipType[chipType]++; + } + } + + ifs.close(); + cmdline = "rm ./tmpFileList.txt"; + system(cmdline.c_str()); + + if(vecChipType.empty()) + { + cerr << "ERROR: There is no per chip json file at " << jsonDir << endl; + exit ( EXIT_FAILURE ); + } + + sort(vecChipType.begin(), vecChipType.end()); + cout << "Available chip types are:" << endl; + for(vector::iterator iter1 = vecChipType.begin(); iter1 != vecChipType.end() - 1; ++iter1) + { + cout << *iter1 << ", "; + } + + cout << vecChipType.back() << endl; + + vector editList; + string chipTypes; + cout << "Please select chip type to edit ('q' to quit; 'n' for new; 'a' for all; separate multiple chip types with ','):" << endl; + cin >> chipTypes; + + if(chipTypes == "q") + { + cout << "Bye." << endl; + exit(0); + } + else if(chipTypes == "n") + { + cout << "New chip type name: "; + cin >> chipTypes; + string chipBase; + cout << "Base chip type (data will be copied to new chip type): "; + cin >> chipBase; + if(chipBase.length() == 0) + { + chipBase = "530"; + } + + string srcName(jsonDir); + srcName += "/args_"; + srcName += chipBase; + srcName += "_analysis.json"; + string desName(jsonDir); + desName += "/args_"; + desName += chipTypes; + desName += "_analysis.json"; + cmdline = "cp "; + cmdline += srcName; + cmdline += " "; + cmdline += desName; + cout << cmdline; + system(cmdline.c_str()); + + srcName = jsonDir; + srcName += "/args_"; + srcName += chipBase; + srcName += "_beadfind.json"; + desName = jsonDir; + desName += "/args_"; + desName += chipTypes; + desName += "_beadfind.json"; + cmdline = "cp "; + cmdline += srcName; + cmdline += " "; + cmdline += desName; + cout << cmdline; + system(cmdline.c_str()); + + editList.push_back(chipTypes); + } + else if(chipTypes == "a") + { + chipTypes = "all chip types"; + editList = vecChipType; + } + else + { + int index = chipTypes.find(","); + while(index > 0) + { + string chipType = chipTypes.substr(0, index); + editList.push_back(chipType); + chipTypes = chipTypes.substr(index + 1, chipTypes.length() - index - 1); + index = chipTypes.find(","); + } + editList.push_back(chipTypes); + } + + string chipType = editList[0]; + + string analysisName(jsonDir); + analysisName += "/args_"; + analysisName += chipType; + analysisName += "_analysis.json"; + string beadfindName(jsonDir); + beadfindName += "/args_"; + beadfindName += chipType; + beadfindName += "_beadfind.json"; + + ifstream ifsa(analysisName.c_str()); + Value jsona; + Reader readera; + readera.parse(ifsa, jsona, false); + ifsa.close(); + + ifstream ifsb(beadfindName.c_str()); + Value jsonb; + Reader readerb; + readerb.parse(ifsb, jsonb, false); + ifsb.close(); + + map modules; + map opts; + vector tnOpts; + Value::Members groupsa = jsona.getMemberNames(); + for(Value::Members::iterator ita1 = groupsa.begin(); ita1 != groupsa.end(); ++ita1) + { + if(*ita1 == "chipType") + { + string chipType0 = jsona["chipType"].asString(); + if(chipType != chipType0) // for new chip type + { + jsona["chipType"] = chipType; + jsonb["chipType"] = chipType; + } + } + else if(*ita1 == "ThumbnailControl") + { + Value::Members itemstn = jsona["ThumbnailControl"].getMemberNames(); + for(Value::Members::iterator itn = itemstn.begin(); itn != itemstn.end(); ++itn) + { + tnOpts.push_back(*itn); + } + } + else + { + modules[*ita1] = 1; + Value::Members itemsa = jsona[*ita1].getMemberNames(); + for(Value::Members::iterator ita2 = itemsa.begin(); ita2 != itemsa.end(); ++ita2) + { + opts[*ita2] = (*ita1); + } + } + } + + Value::Members groupsb = jsonb.getMemberNames(); + for(Value::Members::iterator itb1 = groupsb.begin(); itb1 != groupsb.end(); ++itb1) + { + if(*itb1 != "chipType") + { + if(*itb1 == "ThumbnailControl") + { + Value::Members itemstn = jsonb["ThumbnailControl"].getMemberNames(); + for(Value::Members::iterator itn = itemstn.begin(); itn != itemstn.end(); ++itn) + { + size_t k = 0; + for(; k < tnOpts.size(); ++k) + { + if(tnOpts[k] == (*itn)) + { + break; + } + } + if(k == tnOpts.size()) + { + tnOpts.push_back(*itn); + } + } + + } + else + { + map::iterator iter1 = modules.find(*itb1); + if(iter1 == modules.end()) + { + modules[*itb1] = 2; + Value::Members itemsb = jsonb[*itb1].getMemberNames(); + for(Value::Members::iterator itb2 = itemsb.begin(); itb2 != itemsb.end(); ++itb2) + { + opts[*itb2] = (*itb1); + } + } + else + { + modules[*itb1] = 3; + } + } + } + } + + cout << "Working on chip type " << chipType << endl; + cout << "Which option you want to work on: " << endl + << "\tfor searching: search" << endl + << "\tfor adding new option: new" << endl + << "\tfor listing all options: list" << endl + << "\tfor thumbnail options: thumbnail" << endl; + + int dtype = -1; + string optin; + cin >> optin; + + bool tn = false; + + if(optin == "thumbnail") + { + tn = true; + + cout << "Existing thumbnail options:" << endl; + size_t k = 0; + for(; k < tnOpts.size(); ++k) + { + cout << k + 1 << " - " << tnOpts[k] << endl; + } + + cout << "Please select an option you want to work on (type 0 for adding new option):" << endl; + int n = 0; + cin >> n; + if(n > 0) + { + optin = tnOpts[n - 1]; + } + else + { + cout << "Please type new option name you want to work on (list for listing all options):" << endl; + cin >> optin; + + if(optin == "list") + { + for(map::iterator iter = opts.begin(); iter != opts.end(); ++iter) + { + cout << iter->first << endl; + } + + cout << "Please type the option you want to work on:" << endl; + cin >> optin; + } + } + } + else if(optin == "list") + { + for(map::iterator iter = opts.begin(); iter != opts.end(); ++iter) + { + cout << iter->first << endl; + } + + cout << "Please type the option you want to work on:" << endl; + cin >> optin; + } + else if(optin == "search") + { + cout << "Please type partial of the option word:" << endl; + cin >> optin; + vector names; + for(map::iterator iter = opts.begin(); iter != opts.end(); ++iter) + { + int idxsearch = iter->first.find(optin); + if(idxsearch >= 0) + { + names.push_back(iter->first); + } + } + + if(names.empty()) + { + cout << "There is no option containing " << optin << endl; + cout << "Here is the full list of options:" << endl; + for(map::iterator iter = opts.begin(); iter != opts.end(); ++iter) + { + cout << iter->first << endl; + } + + cout << "Please type the option you want to work on:" << endl; + cin >> optin; + } + else if(names.size() == 1) + { + optin = names[0]; + cout << "Working on option " << optin << endl; + } + else + { + cout << "Here is the list of options containing " << optin << endl; + for(size_t i = 0; i < names.size(); ++i) + { + cout << i << " - " << names[i] << endl; + } + + cout << "Please type the option you want to work on:" << endl; + int k; + cin >> k; + optin = names[k]; + } + } + if(optin == "new") + { + cout << "Please type new option name:" << endl; + cin >> optin; + cout << "Which module you want to add " << optin << " to:" << endl; + for(map::iterator iter = modules.begin(); iter != modules.end(); ++ iter) + { + cout << iter->first << endl; + } + + string mod; + cin >> mod; + opts[optin] = mod; + + cout << "option data type: 0 - bool; 1 - integer; 2 - double; 3 - string; 4 - vector of integers; 5 - vector 0f doubles." << endl; + cin >> dtype; + } + + int idx = -1; + bool val0; + int val1, vali; + double val2, valf; + string val3, val4, val5; + + string mod = opts[optin]; + string mod2 = mod; + if(tn) + { + mod = "ThumbnailControl"; + } + + cout << "option's value:" << endl; + if(dtype < 0) + { + dtype = mapOptType[optin]; + } + + switch (dtype) + { + case OT_BOOL: + cin >> val0; + + if(modules[mod2] == 1) + { + jsona[mod][optin] = val0; + } + else if(modules[mod2] == 2) + { + jsonb[mod][optin] = val0; + } + else if(modules[mod2] == 3) + { + jsona[mod][optin] = val0; + jsonb[mod][optin] = val0; + } + + break; + + case OT_INT: + cin >> val1; + + if(modules[mod2] == 1) + { + jsona[mod][optin] = val1; + } + else if(modules[mod2] == 2) + { + jsonb[mod][optin] = val1; + } + else if(modules[mod2] == 3) + { + jsona[mod][optin] = val1; + jsonb[mod][optin] = val1; + } + + break; + + case OT_DOUBLE: + cin >> val2; + + if(modules[mod2] == 1) + { + jsona[mod][optin] = val2; + } + else if(modules[mod2] == 2) + { + jsonb[mod][optin] = val2; + } + else if(modules[mod2] == 3) + { + jsona[mod][optin] = val2; + jsonb[mod][optin] = val2; + } + + break; + + case OT_STRING: + cin >> val3; + + if(modules[mod2] == 1) + { + jsona[mod][optin] = val3; + } + else if(modules[mod2] == 2) + { + jsonb[mod][optin] = val3; + } + else if(modules[mod2] == 3) + { + jsona[mod][optin] = val3; + jsonb[mod][optin] = val3; + } + + break; + + case OT_VECTOR_INT: + if(modules[mod2] == 1) + { + jsona[mod][optin].clear(); + } + else if(modules[mod2] == 2) + { + jsonb[mod][optin].clear(); + } + else if(modules[mod2] == 3) + { + jsona[mod][optin].clear(); + jsonb[mod][optin].clear(); + } + + cin >> val3; + val4 = val3; + idx = val4.find(","); + while(idx > 0) + { + string s2 = val4.substr(0, idx); + vali = atoi(s2.c_str()); + if(modules[mod2] == 1) + { + jsona[mod][optin].append(vali); + } + else if(modules[mod2] == 2) + { + jsonb[mod][optin].append(vali); + } + else if(modules[mod2] == 3) + { + jsona[mod][optin].append(vali); + jsonb[mod][optin].append(vali); + } + + val4 = val4.substr(idx + 1, val4.length() - idx - 1); + idx = val4.find(","); + } + + vali = atoi(val4.c_str()); + if(modules[mod2] == 1) + { + jsona[mod][optin].append(vali); + } + else if(modules[mod2] == 2) + { + jsonb[mod][optin].append(vali); + } + else if(modules[mod2] == 3) + { + jsona[mod][optin].append(vali); + jsonb[mod][optin].append(vali); + } + + break; + + case OT_VECTOR_DOUBLE: + if(modules[mod2] == 1) + { + jsona[mod][optin].clear(); + } + else if(modules[mod2] == 2) + { + jsonb[mod][optin].clear(); + } + else if(modules[mod2] == 3) + { + jsona[mod][optin].clear(); + jsonb[mod][optin].clear(); + } + + cin >> val3; + val5 = val3; + idx = val5.find(","); + while(idx > 0) + { + string s2 = val5.substr(0, idx); + valf = atof(s2.c_str()); + if(modules[mod2] == 1) + { + jsona[mod][optin].append(valf); + } + else if(modules[mod2] == 2) + { + jsonb[mod][optin].append(valf); + } + else if(modules[mod2] == 3) + { + jsona[mod][optin].append(valf); + jsonb[mod][optin].append(valf); + } + + val5 = val5.substr(idx + 1, val5.length() - idx - 1); + idx = val5.find(","); + } + + valf = atof(val5.c_str()); + if(modules[mod2] == 1) + { + jsona[mod][optin].append(valf); + } + else if(modules[mod2] == 2) + { + jsonb[mod][optin].append(valf); + } + else if(modules[mod2] == 3) + { + jsona[mod][optin].append(valf); + jsonb[mod][optin].append(valf); + } + + break; + + default: + cout << "ERROR: Invalid option data type!" << endl; + return(1); + } + + saveJson(jsona, analysisName); + saveJson(jsonb, beadfindName); + + for(size_t i = 1; i < editList.size(); ++i) + { + chipType = editList[i]; + cout << "Working on chip type " << chipType << endl; + + analysisName = jsonDir; + analysisName += "/args_"; + analysisName += chipType; + analysisName += "_analysis.json"; + beadfindName = jsonDir; + beadfindName += "/args_"; + beadfindName += chipType; + beadfindName += "_beadfind.json"; + + ifstream ifsa2(analysisName.c_str()); + Value jsona2; + Reader readera2; + readera2.parse(ifsa2, jsona2, false); + ifsa2.close(); + + ifstream ifsb2(beadfindName.c_str()); + Value jsonb2; + Reader readerb2; + readerb2.parse(ifsb2, jsonb2, false); + ifsb2.close(); + + switch (dtype) + { + case OT_BOOL: + if(modules[mod2] == 1) + { + jsona2[mod][optin] = val0; + } + else if(modules[mod2] == 2) + { + jsonb2[mod][optin] = val0; + } + else if(modules[mod2] == 3) + { + jsona2[mod][optin] = val0; + jsonb2[mod][optin] = val0; + } + + break; + + case OT_INT: + if(modules[mod2] == 1) + { + jsona2[mod][optin] = val1; + } + else if(modules[mod2] == 2) + { + jsonb2[mod][optin] = val1; + } + else if(modules[mod2] == 3) + { + jsona2[mod][optin] = val1; + jsonb2[mod][optin] = val1; + } + + break; + + case OT_DOUBLE: + if(modules[mod2] == 1) + { + jsona2[mod][optin] = val2; + } + else if(modules[mod2] == 2) + { + jsonb2[mod][optin] = val2; + } + else if(modules[mod2] == 3) + { + jsona2[mod][optin] = val2; + jsonb2[mod][optin] = val2; + } + + break; + + case OT_STRING: + if(modules[mod2] == 1) + { + jsona2[mod][optin] = val3; + } + else if(modules[mod2] == 2) + { + jsonb2[mod][optin] = val3; + } + else if(modules[mod2] == 3) + { + jsona2[mod][optin] = val3; + jsonb2[mod][optin] = val3; + } + + break; + + case OT_VECTOR_INT: + if(modules[mod2] == 1) + { + jsona2[mod][optin].clear(); + } + else if(modules[mod2] == 2) + { + jsonb2[mod][optin].clear(); + } + else if(modules[mod2] == 3) + { + jsona2[mod][optin].clear(); + jsonb2[mod][optin].clear(); + } + + val4 = val3; + idx = val4.find(","); + while(idx > 0) + { + string s2 = val4.substr(0, idx); + vali = atoi(s2.c_str()); + if(modules[mod2] == 1) + { + jsona2[mod][optin].append(vali); + } + else if(modules[mod2] == 2) + { + jsonb2[mod][optin].append(vali); + } + else if(modules[mod2] == 3) + { + jsona2[mod][optin].append(vali); + jsonb2[mod][optin].append(vali); + } + + val4 = val4.substr(idx + 1, val4.length() - idx - 1); + idx = val4.find(","); + } + + vali = atoi(val4.c_str()); + if(modules[mod2] == 1) + { + jsona2[mod][optin].append(vali); + } + else if(modules[mod2] == 2) + { + jsonb2[mod][optin].append(vali); + } + else if(modules[mod2] == 3) + { + jsona2[mod][optin].append(vali); + jsonb2[mod][optin].append(vali); + } + + break; + + case OT_VECTOR_DOUBLE: + if(modules[mod2] == 1) + { + jsona2[mod][optin].clear(); + } + else if(modules[mod2] == 2) + { + jsonb2[mod][optin].clear(); + } + else if(modules[mod2] == 3) + { + jsona2[mod][optin].clear(); + jsonb2[mod][optin].clear(); + } + + val5 = val3; + idx = val5.find(","); + while(idx > 0) + { + string s2 = val5.substr(0, idx); + valf = atof(s2.c_str()); + if(modules[mod2] == 1) + { + jsona2[mod][optin].append(valf); + } + else if(modules[mod2] == 2) + { + jsonb2[mod][optin].append(valf); + } + else if(modules[mod2] == 3) + { + jsona2[mod][optin].append(valf); + jsonb2[mod][optin].append(valf); + } + + val5 = val5.substr(idx + 1, val5.length() - idx - 1); + idx = val5.find(","); + } + + valf = atof(val5.c_str()); + if(modules[mod2] == 1) + { + jsona2[mod][optin].append(valf); + } + else if(modules[mod2] == 2) + { + jsonb2[mod][optin].append(valf); + } + else if(modules[mod2] == 3) + { + jsona2[mod][optin].append(valf); + jsonb2[mod][optin].append(valf); + } + + break; + + default: + break; + } + + saveJson(jsona2, analysisName); + saveJson(jsonb2, beadfindName); + } + + return (0); +} diff --git a/Analysis/TsInputUtil/tsInputUtil.cpp b/Analysis/TsInputUtil/tsInputUtil.cpp index 622e9781..87aa4609 100644 --- a/Analysis/TsInputUtil/tsInputUtil.cpp +++ b/Analysis/TsInputUtil/tsInputUtil.cpp @@ -10,7 +10,7 @@ #include #include "json/json.h" -#include "Utils.h"" +#include "Utils.h" #include "OptArgs.h" #define TS_INPUT_UTIL_VERSION "1.0.0" @@ -165,11 +165,11 @@ int main(int argc, const char *argv[]) jsonBase["BkgModelControlOpts"]["mixed-stringency"]["value"] = 0.5; jsonBase["BkgModelControlOpts"]["mixed-stringency"]["min"] = ""; jsonBase["BkgModelControlOpts"]["mixed-stringency"]["max"] = ""; - jsonBase["BkgModelControlOpts"]["sigproc-regional-smoothing-alpha"]["type"] = OT_FLOAT; + jsonBase["BkgModelControlOpts"]["sigproc-regional-smoothing-alpha"]["type"] = OT_DOUBLE; jsonBase["BkgModelControlOpts"]["sigproc-regional-smoothing-alpha"]["value"] = 1.0; jsonBase["BkgModelControlOpts"]["sigproc-regional-smoothing-alpha"]["min"] = ""; jsonBase["BkgModelControlOpts"]["sigproc-regional-smoothing-alpha"]["max"] = ""; - jsonBase["BkgModelControlOpts"]["sigproc-regional-smoothing-gamma"]["type"] = OT_FLOAT; + jsonBase["BkgModelControlOpts"]["sigproc-regional-smoothing-gamma"]["type"] = OT_DOUBLE; jsonBase["BkgModelControlOpts"]["sigproc-regional-smoothing-gamma"]["value"] = 1.0; jsonBase["BkgModelControlOpts"]["sigproc-regional-smoothing-gamma"]["min"] = ""; jsonBase["BkgModelControlOpts"]["sigproc-regional-smoothing-gamma"]["max"] = ""; @@ -177,7 +177,7 @@ int main(int argc, const char *argv[]) jsonBase["BkgModelControlOpts"]["restart-region-params-file"]["value"] = ""; jsonBase["BkgModelControlOpts"]["restart-region-params-file"]["min"] = ""; jsonBase["BkgModelControlOpts"]["restart-region-params-file"]["max"] = ""; - jsonBase["BkgModelControlOpts"]["bkg-washout-threshold"]["type"] = OT_FLOAT; + jsonBase["BkgModelControlOpts"]["bkg-washout-threshold"]["type"] = OT_DOUBLE; jsonBase["BkgModelControlOpts"]["bkg-washout-threshold"]["value"] = 2.0; jsonBase["BkgModelControlOpts"]["bkg-washout-threshold"]["min"] = ""; jsonBase["BkgModelControlOpts"]["bkg-washout-threshold"]["max"] = ""; @@ -185,7 +185,7 @@ int main(int argc, const char *argv[]) jsonBase["BkgModelControlOpts"]["bkg-washout-flow-detection"]["value"] = 6; jsonBase["BkgModelControlOpts"]["bkg-washout-flow-detection"]["min"] = ""; jsonBase["BkgModelControlOpts"]["bkg-washout-flow-detection"]["max"] = ""; - jsonBase["GpuControlOpts"]["gpuworkload"]["type"] = OT_FLOAT; + jsonBase["GpuControlOpts"]["gpuworkload"]["type"] = OT_DOUBLE; jsonBase["GpuControlOpts"]["gpuworkload"]["value"] = 1.0; jsonBase["GpuControlOpts"]["gpuworkload"]["min"] = 0.0; jsonBase["GpuControlOpts"]["gpuworkload"]["max"] = 1.0; @@ -269,6 +269,14 @@ int main(int argc, const char *argv[]) jsonBase["GpuControlOpts"]["gpu-num-history-flows"]["value"] = 10; jsonBase["GpuControlOpts"]["gpu-num-history-flows"]["min"] = ""; jsonBase["GpuControlOpts"]["gpu-num-history-flows"]["max"] = ""; + jsonBase["GpuControlOpts"]["gpu-force-multi-flow-fit"]["type"] = OT_BOOL; + jsonBase["GpuControlOpts"]["gpu-force-multi-flow-fit"]["value"] = false; + jsonBase["GpuControlOpts"]["gpu-force-multi-flow-fit"]["min"] = ""; + jsonBase["GpuControlOpts"]["gpu-force-multi-flow-fit"]["max"] = ""; + jsonBase["GpuControlOpts"]["gpu-memory-per-proc"]["type"] = OT_INT; + jsonBase["GpuControlOpts"]["gpu-memory-per-proc"]["value"] = 0; + jsonBase["GpuControlOpts"]["gpu-memory-per-proc"]["min"] = ""; + jsonBase["GpuControlOpts"]["gpu-memory-per-proc"]["max"] = ""; jsonBase["SignalProcessingBlockControl"]["wells-compression"]["type"] = OT_INT; jsonBase["SignalProcessingBlockControl"]["wells-compression"]["value"] = 0; jsonBase["SignalProcessingBlockControl"]["wells-compression"]["min"] = 0; @@ -367,6 +375,10 @@ int main(int argc, const char *argv[]) jsonBase["BeadfindControlOpts"]["use-beadmask"]["value"] = ""; jsonBase["BeadfindControlOpts"]["use-beadmask"]["min"] = ""; jsonBase["BeadfindControlOpts"]["use-beadmask"]["max"] = ""; + jsonBase["BeadfindControlOpts"]["exclusion-mask"]["type"] = OT_STRING; + jsonBase["BeadfindControlOpts"]["exclusion-mask"]["value"] = ""; + jsonBase["BeadfindControlOpts"]["exclusion-mask"]["min"] = ""; + jsonBase["BeadfindControlOpts"]["exclusion-mask"]["max"] = ""; jsonBase["BeadfindControlOpts"]["beadmask-categorized"]["type"] = OT_BOOL; jsonBase["BeadfindControlOpts"]["beadmask-categorized"]["value"] = false; jsonBase["BeadfindControlOpts"]["beadmask-categorized"]["min"] = ""; @@ -387,7 +399,7 @@ int main(int argc, const char *argv[]) jsonBase["BeadfindControlOpts"]["beadfind-sdasbf"]["value"] = true; jsonBase["BeadfindControlOpts"]["beadfind-sdasbf"]["min"] = ""; jsonBase["BeadfindControlOpts"]["beadfind-sdasbf"]["max"] = ""; - jsonBase["BeadfindControlOpts"]["beadfind-bfmult"]["type"] = OT_FLOAT; + jsonBase["BeadfindControlOpts"]["beadfind-bfmult"]["type"] = OT_DOUBLE; jsonBase["BeadfindControlOpts"]["beadfind-bfmult"]["value"] = 1.0; jsonBase["BeadfindControlOpts"]["beadfind-bfmult"]["min"] = ""; jsonBase["BeadfindControlOpts"]["beadfind-bfmult"]["max"] = ""; @@ -407,11 +419,11 @@ int main(int argc, const char *argv[]) jsonBase["BeadfindControlOpts"]["beadfind-min-tf-snr"]["value"] = 7.0; jsonBase["BeadfindControlOpts"]["beadfind-min-tf-snr"]["min"] = ""; jsonBase["BeadfindControlOpts"]["beadfind-min-tf-snr"]["max"] = ""; - jsonBase["BeadfindControlOpts"]["beadfind-tf-min-peak"]["type"] = OT_FLOAT; + jsonBase["BeadfindControlOpts"]["beadfind-tf-min-peak"]["type"] = OT_DOUBLE; jsonBase["BeadfindControlOpts"]["beadfind-tf-min-peak"]["value"] = 40.0; jsonBase["BeadfindControlOpts"]["beadfind-tf-min-peak"]["min"] = ""; jsonBase["BeadfindControlOpts"]["beadfind-tf-min-peak"]["max"] = ""; - jsonBase["BeadfindControlOpts"]["beadfind-lib-min-peak"]["type"] = OT_FLOAT; + jsonBase["BeadfindControlOpts"]["beadfind-lib-min-peak"]["type"] = OT_DOUBLE; jsonBase["BeadfindControlOpts"]["beadfind-lib-min-peak"]["value"] = 10.0; jsonBase["BeadfindControlOpts"]["beadfind-lib-min-peak"]["min"] = ""; jsonBase["BeadfindControlOpts"]["beadfind-lib-min-peak"]["max"] = ""; @@ -427,10 +439,6 @@ int main(int argc, const char *argv[]) jsonBase["BeadfindControlOpts"]["beadfind-skip-sd-recover"]["value"] = 1; jsonBase["BeadfindControlOpts"]["beadfind-skip-sd-recover"]["min"] = ""; jsonBase["BeadfindControlOpts"]["beadfind-skip-sd-recover"]["max"] = ""; - jsonBase["BeadfindControlOpts"]["beadfind-thumbnail"]["type"] = OT_INT; - jsonBase["BeadfindControlOpts"]["beadfind-thumbnail"]["value"] = 0; - jsonBase["BeadfindControlOpts"]["beadfind-thumbnail"]["min"] = ""; - jsonBase["BeadfindControlOpts"]["beadfind-thumbnail"]["max"] = ""; jsonBase["BeadfindControlOpts"]["beadfind-sep-ref"]["type"] = OT_BOOL; jsonBase["BeadfindControlOpts"]["beadfind-sep-ref"]["value"] = false; jsonBase["BeadfindControlOpts"]["beadfind-sep-ref"]["min"] = ""; @@ -583,7 +591,7 @@ int main(int argc, const char *argv[]) jsonBase["ImageControlOpts"]["col-doubles-xtalk-correct"]["value"] = false; jsonBase["ImageControlOpts"]["col-doubles-xtalk-correct"]["min"] = ""; jsonBase["ImageControlOpts"]["col-doubles-xtalk-correct"]["max"] = ""; - jsonBase["ImageControlOpts"]["pair-xtalk-coeff"]["type"] = OT_FLOAT; + jsonBase["ImageControlOpts"]["pair-xtalk-coeff"]["type"] = OT_DOUBLE; jsonBase["ImageControlOpts"]["pair-xtalk-coeff"]["value"] = 0.0; jsonBase["ImageControlOpts"]["pair-xtalk-coeff"]["min"] = ""; jsonBase["ImageControlOpts"]["pair-xtalk-coeff"]["max"] = ""; @@ -591,7 +599,7 @@ int main(int argc, const char *argv[]) jsonBase["ImageControlOpts"]["fluid-potential-correct"]["value"] = false; jsonBase["ImageControlOpts"]["fluid-potential-correct"]["min"] = ""; jsonBase["ImageControlOpts"]["fluid-potential-correct"]["max"] = ""; - jsonBase["ImageControlOpts"]["fluid-potential-threshold"]["type"] = OT_FLOAT; + jsonBase["ImageControlOpts"]["fluid-potential-threshold"]["type"] = OT_DOUBLE; jsonBase["ImageControlOpts"]["fluid-potential-threshold"]["value"] = 1.0; jsonBase["ImageControlOpts"]["fluid-potential-threshold"]["min"] = ""; jsonBase["ImageControlOpts"]["fluid-potential-threshold"]["max"] = ""; @@ -599,6 +607,10 @@ int main(int argc, const char *argv[]) jsonBase["ImageControlOpts"]["corr-noise-correct"]["value"] = true; jsonBase["ImageControlOpts"]["corr-noise-correct"]["min"] = ""; jsonBase["ImageControlOpts"]["corr-noise-correct"]["max"] = ""; + jsonBase["ImageControlOpts"]["mask-datacollect-exclude-regions"]["type"] = OT_BOOL; + jsonBase["ImageControlOpts"]["mask-datacollect-exclude-regions"]["value"] = false; + jsonBase["ImageControlOpts"]["mask-datacollect-exclude-regions"]["min"] = ""; + jsonBase["ImageControlOpts"]["mask-datacollect-exclude-regions"]["max"] = ""; jsonBase["ModuleControlOpts"]["bfonly"]["type"] = OT_BOOL; jsonBase["ModuleControlOpts"]["bfonly"]["value"] = false; jsonBase["ModuleControlOpts"]["bfonly"]["min"] = ""; @@ -705,11 +717,11 @@ int main(int argc, const char *argv[]) jsonBase["SystemContext"]["wells-save-as-ushort"]["value"] = true; jsonBase["SystemContext"]["wells-save-as-ushort"]["min"] = ""; jsonBase["SystemContext"]["wells-save-as-ushort"]["max"] = ""; - jsonBase["SystemContext"]["wells-convert-low"]["type"] = OT_FLOAT; + jsonBase["SystemContext"]["wells-convert-low"]["type"] = OT_DOUBLE; jsonBase["SystemContext"]["wells-convert-low"]["value"] = -5.0; jsonBase["SystemContext"]["wells-convert-low"]["min"] = ""; jsonBase["SystemContext"]["wells-convert-low"]["max"] = ""; - jsonBase["SystemContext"]["wells-convert-high"]["type"] = OT_FLOAT; + jsonBase["SystemContext"]["wells-convert-high"]["type"] = OT_DOUBLE; jsonBase["SystemContext"]["wells-convert-high"]["value"] = 28.0; jsonBase["SystemContext"]["wells-convert-high"]["min"] = ""; jsonBase["SystemContext"]["wells-convert-high"]["max"] = ""; @@ -741,19 +753,19 @@ int main(int argc, const char *argv[]) jsonBase["GlobalDefaultsForBkgModel"]["barcode-spec-file"]["value"] = ""; jsonBase["GlobalDefaultsForBkgModel"]["barcode-spec-file"]["min"] = ""; jsonBase["GlobalDefaultsForBkgModel"]["barcode-spec-file"]["max"] = ""; - jsonBase["LocalSigProcControl"]["bkg-kmult-adj-low-hi"]["type"] = OT_FLOAT; + jsonBase["LocalSigProcControl"]["bkg-kmult-adj-low-hi"]["type"] = OT_DOUBLE; jsonBase["LocalSigProcControl"]["bkg-kmult-adj-low-hi"]["value"] = 2.0; jsonBase["LocalSigProcControl"]["bkg-kmult-adj-low-hi"]["min"] = ""; jsonBase["LocalSigProcControl"]["bkg-kmult-adj-low-hi"]["max"] = ""; - jsonBase["LocalSigProcControl"]["kmult-low-limit"]["type"] = OT_FLOAT; + jsonBase["LocalSigProcControl"]["kmult-low-limit"]["type"] = OT_DOUBLE; jsonBase["LocalSigProcControl"]["kmult-low-limit"]["value"] = 0.65; jsonBase["LocalSigProcControl"]["kmult-low-limit"]["min"] = ""; jsonBase["LocalSigProcControl"]["kmult-low-limit"]["max"] = ""; - jsonBase["LocalSigProcControl"]["kmult-hi-limit"]["type"] = OT_FLOAT; + jsonBase["LocalSigProcControl"]["kmult-hi-limit"]["type"] = OT_DOUBLE; jsonBase["LocalSigProcControl"]["kmult-hi-limit"]["value"] = 1.75; jsonBase["LocalSigProcControl"]["kmult-hi-limit"]["min"] = ""; jsonBase["LocalSigProcControl"]["kmult-hi-limit"]["max"] = ""; - jsonBase["LocalSigProcControl"]["bkg-copy-stringency"]["type"] = OT_FLOAT; + jsonBase["LocalSigProcControl"]["bkg-copy-stringency"]["type"] = OT_DOUBLE; jsonBase["LocalSigProcControl"]["bkg-copy-stringency"]["value"] = 1.0; jsonBase["LocalSigProcControl"]["bkg-copy-stringency"]["min"] = ""; jsonBase["LocalSigProcControl"]["bkg-copy-stringency"]["max"] = ""; @@ -789,19 +801,19 @@ int main(int argc, const char *argv[]) jsonBase["LocalSigProcControl"]["barcode-debug"]["value"] = false; jsonBase["LocalSigProcControl"]["barcode-debug"]["min"] = ""; jsonBase["LocalSigProcControl"]["barcode-debug"]["max"] = ""; - jsonBase["LocalSigProcControl"]["barcode-radius"]["type"] = OT_FLOAT; + jsonBase["LocalSigProcControl"]["barcode-radius"]["type"] = OT_DOUBLE; jsonBase["LocalSigProcControl"]["barcode-radius"]["value"] = 0.75; jsonBase["LocalSigProcControl"]["barcode-radius"]["min"] = ""; jsonBase["LocalSigProcControl"]["barcode-radius"]["max"] = ""; - jsonBase["LocalSigProcControl"]["barcode-tie"]["type"] = OT_FLOAT; + jsonBase["LocalSigProcControl"]["barcode-tie"]["type"] = OT_DOUBLE; jsonBase["LocalSigProcControl"]["barcode-tie"]["value"] = 0.5; jsonBase["LocalSigProcControl"]["barcode-tie"]["min"] = ""; jsonBase["LocalSigProcControl"]["barcode-tie"]["max"] = ""; - jsonBase["LocalSigProcControl"]["barcode-penalty"]["type"] = OT_FLOAT; + jsonBase["LocalSigProcControl"]["barcode-penalty"]["type"] = OT_DOUBLE; jsonBase["LocalSigProcControl"]["barcode-penalty"]["value"] = 2000.0; jsonBase["LocalSigProcControl"]["barcode-penalty"]["min"] = ""; jsonBase["LocalSigProcControl"]["barcode-penalty"]["max"] = ""; - jsonBase["LocalSigProcControl"]["kmult-penalty"]["type"] = OT_FLOAT; + jsonBase["LocalSigProcControl"]["kmult-penalty"]["type"] = OT_DOUBLE; jsonBase["LocalSigProcControl"]["kmult-penalty"]["value"] = 100.0; jsonBase["LocalSigProcControl"]["kmult-penalty"]["min"] = ""; jsonBase["LocalSigProcControl"]["kmult-penalty"]["max"] = ""; @@ -825,11 +837,11 @@ int main(int argc, const char *argv[]) jsonBase["LocalSigProcControl"]["bkg-exp-tail-tau-adj"]["value"] = true; jsonBase["LocalSigProcControl"]["bkg-exp-tail-tau-adj"]["min"] = ""; jsonBase["LocalSigProcControl"]["bkg-exp-tail-tau-adj"]["max"] = ""; - jsonBase["LocalSigProcControl"]["bkg-exp-tail-bkg-limit"]["type"] = OT_FLOAT; + jsonBase["LocalSigProcControl"]["bkg-exp-tail-bkg-limit"]["type"] = OT_DOUBLE; jsonBase["LocalSigProcControl"]["bkg-exp-tail-bkg-limit"]["value"] = 0.2; jsonBase["LocalSigProcControl"]["bkg-exp-tail-bkg-limit"]["min"] = ""; jsonBase["LocalSigProcControl"]["bkg-exp-tail-bkg-limit"]["max"] = ""; - jsonBase["LocalSigProcControl"]["bkg-exp-tail-bkg-lower"]["type"] = OT_FLOAT; + jsonBase["LocalSigProcControl"]["bkg-exp-tail-bkg-lower"]["type"] = OT_DOUBLE; jsonBase["LocalSigProcControl"]["bkg-exp-tail-bkg-lower"]["value"] = 10.0; jsonBase["LocalSigProcControl"]["bkg-exp-tail-bkg-lower"]["min"] = ""; jsonBase["LocalSigProcControl"]["bkg-exp-tail-bkg-lower"]["max"] = ""; @@ -861,7 +873,7 @@ int main(int argc, const char *argv[]) jsonBase["LocalSigProcControl"]["vectorize"]["value"] = true; jsonBase["LocalSigProcControl"]["vectorize"]["min"] = ""; jsonBase["LocalSigProcControl"]["vectorize"]["max"] = ""; - jsonBase["LocalSigProcControl"]["bkg-ampl-lower-limit"]["type"] = OT_FLOAT; + jsonBase["LocalSigProcControl"]["bkg-ampl-lower-limit"]["type"] = OT_DOUBLE; jsonBase["LocalSigProcControl"]["bkg-ampl-lower-limit"]["value"] = 0.001; jsonBase["LocalSigProcControl"]["bkg-ampl-lower-limit"]["min"] = ""; jsonBase["LocalSigProcControl"]["bkg-ampl-lower-limit"]["max"] = ""; diff --git a/Analysis/Util/NumericalComparison.h b/Analysis/Util/NumericalComparison.h index b7d8d13a..8fed0ec1 100644 --- a/Analysis/Util/NumericalComparison.h +++ b/Analysis/Util/NumericalComparison.h @@ -4,6 +4,7 @@ #include #include #include +#include #include "SampleStats.h" #define NC_CORRELATION_EPSILON .00001 @@ -48,10 +49,10 @@ class NumericalComparison { * particiular x & y are closer than epsilon, false otherwise. */ bool AddPair(const T &x, const T &y) { - if (isnan(x) && isnan(y)) { + if (std::isnan(x) && std::isnan(y)) { return true; } - if (isinf(x) && isinf(y)) { + if (std::isinf(x) && std::isinf(y)) { return true; } bool closeEnough = true; diff --git a/Analysis/Util/Utils.cpp b/Analysis/Util/Utils.cpp index 2dc13dda..f6c08772 100644 --- a/Analysis/Util/Utils.cpp +++ b/Analysis/Util/Utils.cpp @@ -970,4 +970,129 @@ int GetAbsoluteFreeSystemMemoryInKB() return freeMem; } +// ----------------- + + +void expandBaseSymbol(char nuc, std::vector& nuc_ensemble) +{ + nuc_ensemble.assign(4, false); + nuc = toupper(nuc); + + switch(nuc) { + case 'A': nuc_ensemble[0] = true; break; + case 'C': nuc_ensemble[1] = true; break; + case 'G': nuc_ensemble[2] = true; break; + case 'T': nuc_ensemble[3] = true; break; + case 'U': nuc_ensemble[3] = true; break; + + case 'W': nuc_ensemble[0] = true; nuc_ensemble[3] = true; break; + case 'S': nuc_ensemble[1] = true; nuc_ensemble[2] = true; break; + case 'M': nuc_ensemble[0] = true; nuc_ensemble[1] = true; break; + case 'K': nuc_ensemble[2] = true; nuc_ensemble[3] = true; break; + case 'R': nuc_ensemble[0] = true; nuc_ensemble[2] = true; break; + case 'Y': nuc_ensemble[1] = true; nuc_ensemble[3] = true; break; + + case 'B': nuc_ensemble[1] = true; nuc_ensemble[2] = true; nuc_ensemble[3] = true; break; + case 'D': nuc_ensemble[0] = true; nuc_ensemble[2] = true; nuc_ensemble[3] = true; break; + case 'H': nuc_ensemble[0] = true; nuc_ensemble[1] = true; nuc_ensemble[3] = true; break; + case 'I': nuc_ensemble[0] = true; nuc_ensemble[1] = true; nuc_ensemble[3] = true; break; + case 'V': nuc_ensemble[0] = true; nuc_ensemble[1] = true; nuc_ensemble[2] = true; break; + + case 'N': nuc_ensemble.assign(4, true); break; + } +} + +char contractNucSymbol(const std::vector& nuc_ensemble) +{ + if (nuc_ensemble.size() != 4) + return 'Z'; + + unsigned int weight = 0; + for (unsigned int i=0; i<4; ++i) + if (nuc_ensemble[i]) + ++weight; + + if (weight == 0) + return 'Z'; + + else if (weight==4) + return 'N'; + + else if (weight == 3){ + if (not nuc_ensemble[0]) + return 'B'; + else if (not nuc_ensemble[1]) + return 'D'; + else if (not nuc_ensemble[2]) + return 'H'; + else // if (not nuc_ensemble[3]) + return 'V'; + } + + else if (weight == 2){ + if (nuc_ensemble[0] and nuc_ensemble[3]) + return 'W'; + else if (nuc_ensemble[1] and nuc_ensemble[2]) + return 'S'; + else if (nuc_ensemble[0] and nuc_ensemble[1]) + return 'M'; + else if (nuc_ensemble[2] and nuc_ensemble[3]) + return 'K'; + else if (nuc_ensemble[0] and nuc_ensemble[2]) + return 'R'; + else //if (nuc_ensemble[1] and nuc_ensemble[3]) + return 'Y'; + } + + else if (nuc_ensemble[0]) + return 'A'; + else if (nuc_ensemble[1]) + return 'C'; + else if (nuc_ensemble[2]) + return 'G'; + else // if (nuc_ensemble[3]) + return 'T'; +} + + +bool isBaseMatch(char nuc1, char nuc2) +{ + std::vector ensemble1, ensemble2; + expandBaseSymbol(nuc1, ensemble1); + expandBaseSymbol(nuc2, ensemble2); + + /* / ---- XXX + cerr << "["; + for (unsigned int i=0; i ensemble1, ensemble2; + expandBaseSymbol(nuc1, ensemble1); + expandBaseSymbol(nuc2, ensemble2); + + for (unsigned int i=0; i #include #include + #ifndef ALIGNSTATS_IGNORE #include "SpecialDataTypes.h" #include "LinuxCompat.h" #endif + // Transition states used in Analysis main function for progress tracking #define WELL_TO_IMAGE 1 #define IMAGE_TO_SIGNAL 2 @@ -72,6 +74,12 @@ int GetFreeSystemMem(); int GetCachedSystemMem(); int GetSystemMemInBuffers(); +// --- Mapping of base ambiguity symbols + +void expandBaseSymbol(char nuc, std::vector& nuc_ensemble); +char contractNucSymbol(const std::vector& nuc_ensemble); +bool isBaseMatch(char nuc1, char nuc2); +char getMatchSymbol(char nuc1, char nuc2); //string utils int count_char (std::string s, char c); @@ -283,5 +291,4 @@ T fast_median(T* start, size_t num_elements) { return (*middle + *(middle -1)) /2.0f; } - #endif // UTILS_H diff --git a/Analysis/VariantCaller/BAMWalkerEngine.cpp b/Analysis/VariantCaller/BAMWalkerEngine.cpp index 66ee1aad..6f77943d 100644 --- a/Analysis/VariantCaller/BAMWalkerEngine.cpp +++ b/Analysis/VariantCaller/BAMWalkerEngine.cpp @@ -199,16 +199,30 @@ void BAMWalkerEngine::InitializeBAMs(const ReferenceReader& ref_reader, const ve tmap_version_ += ", "; tmap_version_ += *I; } - } - - bool BAMWalkerEngine::EligibleForReadRemoval() { return alignments_first_ and alignments_first_->read_number+100 < first_useful_read_; } +// Determine the eligibility for read removal for target-pileup +//@TODO: Should depend on first_useful_read_ only. +bool ConsensusBAMWalkerEngine::EligibleForTargetBasedReadRemoval() +{ + if (EligibleForReadRemoval()){ + if (positions_in_progress_.empty()){ + return (((int) alignments_first_->alignment.RefID < next_target_->chr) + or (((int) alignments_first_->alignment.RefID == next_target_->chr) + and (alignments_first_->end < (long int) next_target_->begin))); + }else{ + return (((int) alignments_first_->alignment.RefID < positions_in_progress_.begin()->chr) + or (((int) alignments_first_->alignment.RefID == positions_in_progress_.begin()->chr) + and (alignments_first_->end < (long int) positions_in_progress_.begin()->target_begin))); + } + } + return false; +} void BAMWalkerEngine::RequestReadRemovalTask(Alignment*& removal_list) { @@ -226,6 +240,44 @@ void BAMWalkerEngine::RequestReadRemovalTask(Alignment*& removal_list) list_end->next = NULL; } +// Request read removal for target-pileup. +//@TODO: Should depend on first_useful_read_ only. +void ConsensusBAMWalkerEngine::RequestTargetBasedReadRemovalTask(Alignment*& removal_list) +{ + removal_list = alignments_first_; + + Alignment *list_end = removal_list; + + if (positions_in_progress_.empty()){ + while (alignments_first_ and (alignments_first_->read_number < first_useful_read_)){ + if (((int) alignments_first_->alignment.RefID > next_target_->chr) + or (((int) alignments_first_->alignment.RefID == next_target_->chr) + and (alignments_first_->end >= (long int) next_target_->begin))){ + break; + } + list_end = alignments_first_; + alignments_first_ = alignments_first_->next; + } + } + else{ + while (alignments_first_ and (alignments_first_->read_number < first_useful_read_)){ + if (((int) alignments_first_->alignment.RefID > positions_in_progress_.begin()->chr) + or (((int) alignments_first_->alignment.RefID == positions_in_progress_.begin()->chr) + and (alignments_first_->end >= (long int) positions_in_progress_.begin()->target_begin))){ + break; + } + list_end = alignments_first_; + alignments_first_ = alignments_first_->next; + } + } + if (list_end == removal_list) + removal_list = NULL; + else + list_end->next = NULL; +} + + + void BAMWalkerEngine::openDepth(const string& depth_file) { pthread_mutex_init(&mutexdepth, NULL); depth_out.open(depth_file.c_str(), ofstream::out); prevRefID = -1; prevEndPos = -1; @@ -275,6 +327,9 @@ void BAMWalkerEngine::processDepth(BamAlignment& alignment, TargetsManager* targ // back up a couple of targets because reads can span multiple targets. if (curr_target > targets_manager->merged.begin()) {--curr_target;} if (curr_target > targets_manager->merged.begin()) {--curr_target;} + int read_count = 1; + alignment.GetTag("ZR", read_count); + for (long int pos = alignment.Position + 1; (pos <= alignment.GetEndPosition()); ++pos) { while (curr_target != targets_manager->merged.end() && (alignment.RefID > curr_target->chr || (alignment.RefID == curr_target->chr && pos - 1 >= curr_target->end))) { ++curr_target; @@ -286,11 +341,11 @@ void BAMWalkerEngine::processDepth(BamAlignment& alignment, TargetsManager* targ std::map ::iterator depth_iter = depth_map.find(pos); if (depth_iter == depth_map.end()) { depth_map[pos] = 0; - if (skip_positions.find(pos) == skip_positions.end()) {depth_map[pos]++;} + if (skip_positions.find(pos) == skip_positions.end()) {depth_map[pos] += read_count;} } else { if (skip_positions.find(pos) == skip_positions.end()) { - depth_iter->second++; + depth_iter->second += read_count; } } pthread_mutex_unlock (&mutexdepth); @@ -330,6 +385,8 @@ bool compare_alignments(Alignment* const A, Alignment* const B) { return result; } + + void BAMWalkerEngine::SaveAlignments(Alignment*& removal_list, VariantCallerContext& vc, vector::iterator& depth_target) { if (removal_list == NULL) {return;} @@ -364,9 +421,13 @@ void BAMWalkerEngine::SaveAlignments(Alignment*& removal_list, VariantCallerCont prevRefID = current_read->alignment.RefID; prevEndPos = current_read->alignment.GetEndPosition(); if (bam_writing_enabled_) { - current_read->alignment.RemoveTag("ZM"); - current_read->alignment.RemoveTag("ZP"); - current_read->alignment.RemoveTag("PG"); + // Remove some tags to make the processed bam file lighter. + const vector unwanted_tags = {"ZM", "ZP", "PG", "ZS"}; + for (vector::const_iterator tag_it = unwanted_tags.begin(); tag_it != unwanted_tags.end(); ++tag_it){ + if (current_read->alignment.HasTag(*tag_it)){ + current_read->alignment.RemoveTag(*tag_it); + } + } bam_writer_.SaveAlignment(current_read->alignment); } } @@ -376,7 +437,6 @@ void BAMWalkerEngine::SaveAlignments(Alignment*& removal_list, VariantCallerCont void BAMWalkerEngine::FinishReadRemovalTask(Alignment* removal_list, int recycle_limit) { pthread_mutex_lock(&recycle_mutex_); - while (removal_list) { Alignment *excess = removal_list; @@ -590,29 +650,28 @@ void BAMWalkerEngine::FinishReadProcessingTask(Alignment* new_read, bool success void BAMWalkerEngine::SetupPositionTicket(list::iterator& position_ticket) const { if (position_ticket->begin == NULL) {return;} +/* Alignment* tmp_begin_ = position_ticket->begin; - while (tmp_begin_ and ( (tmp_begin_->alignment.RefID == next_target_->chr and tmp_begin_->end <= next_position_) or tmp_begin_->alignment.RefID < next_target_->chr) and tmp_begin_->processed) tmp_begin_ = tmp_begin_->next; - Alignment* tmp_end_ = tmp_begin_; - while (tmp_end_ and ( (tmp_end_->alignment.RefID == next_target_->chr and tmp_end_->original_position <= next_position_) or tmp_end_->alignment.RefID < next_target_->chr) and tmp_end_->processed) tmp_end_ = tmp_end_->next; +*/ //positions_in_progress_.push_back(PositionInProgress()); //position_ticket = positions_in_progress_.end(); //--position_ticket; position_ticket->chr = next_target_->chr; position_ticket->pos = next_position_; position_ticket->target_end = next_target_->end; - position_ticket->begin = tmp_begin_; - position_ticket->end = tmp_end_; + //position_ticket->begin = tmp_begin_; + //position_ticket->end = tmp_end_; position_ticket->start_time = time(NULL); //first_excess_read_ = tmp_end_->read_number; } @@ -642,6 +701,7 @@ void BAMWalkerEngine::BeginPositionProcessingTask(list::iter --position_ticket; position_ticket->chr = next_target_->chr; position_ticket->pos = next_position_; + position_ticket->target_begin = next_target_->begin; position_ticket->target_end = next_target_->end; position_ticket->begin = tmp_begin_; position_ticket->end = tmp_end_; @@ -705,7 +765,6 @@ void BAMWalkerEngine::FinishPositionProcessingTask(list::ite } - int BAMWalkerEngine::GetRecentUnmergedTarget() { MergedTarget *my_next_target = next_target_; @@ -716,13 +775,13 @@ int BAMWalkerEngine::GetRecentUnmergedTarget() } -bool BAMWalkerEngine::MemoryContention() +bool BAMWalkerEngine::MemoryContention(int max_num_reads) { if (positions_in_progress_.empty()) return false; if (not alignments_first_) return false; - if (read_counter_ - alignments_first_->read_number < 50000) + if (read_counter_ - alignments_first_->read_number < max_num_reads) return false; return true; } @@ -742,6 +801,148 @@ void BAMWalkerEngine::PrintStatus() << " recycle=" << recycle_size_ << endl; } +void ConsensusBAMWalkerEngine::Initialize(const ReferenceReader& ref_reader, TargetsManager& targets_manager, + const vector& bam_filenames, const string& postprocessed_bam, int px, const string& consensus_bam) +{ + this->BAMWalkerEngine::Initialize(ref_reader, targets_manager, bam_filenames, "", px); // no postprocessed_bam + write_consensus_bam_ = not consensus_bam.empty(); + if (not write_consensus_bam_){ + // It seems that I don't want to output consensus bam. As you wish. + return; + } + pthread_mutex_init(&aln_no_needed_consensus_bam_writer_mutex_, NULL); + pthread_mutex_init(&aln_needed_consensus_bam_writer_mutex_, NULL); + SamHeader tmp_header = bam_header_; + //tmp_header.Comments.clear(); + //tmp_header.Programs.Clear(); + aln_no_needed_consensus_bam_writer_.SetCompressionMode(BamWriter::Compressed); + aln_no_needed_consensus_bam_writer_.SetNumThreads(4); + if (not aln_no_needed_consensus_bam_writer_.Open(consensus_bam + ".aln_not_needed.bam", tmp_header, bam_reader_.GetReferenceData())) { + cerr << "ERROR: Could not open consensus BAM file for writing : " << aln_no_needed_consensus_bam_writer_.GetErrorString(); + exit(1); + } + aln_needed_consensus_bam_writer_.SetCompressionMode(BamWriter::Compressed); + aln_needed_consensus_bam_writer_.SetNumThreads(4); + if (not aln_needed_consensus_bam_writer_.Open(consensus_bam + ".aln_needed.bam", tmp_header, bam_reader_.GetReferenceData())) { + cerr << "ERROR: Could not open consensus BAM file for writing : " << aln_needed_consensus_bam_writer_.GetErrorString(); + exit(1); + } +} + +void ConsensusBAMWalkerEngine::Close() +{ + this->BAMWalkerEngine::Close(); + if (write_consensus_bam_){ + aln_no_needed_consensus_bam_writer_.Close(); + aln_needed_consensus_bam_writer_.Close(); + } + pthread_mutex_destroy(&aln_no_needed_consensus_bam_writer_mutex_); + pthread_mutex_destroy(&aln_needed_consensus_bam_writer_mutex_); +} +void ConsensusBAMWalkerEngine::SaveConsensusAlignments(Alignment* const &read_list, Alignment* const &aln_needed_read_list) +{ + if (not write_consensus_bam_){ + return; + } + if (read_list != NULL){ + // Process linked list + pthread_mutex_lock (&aln_no_needed_consensus_bam_writer_mutex_); + for (Alignment *current_read = read_list; current_read; current_read = current_read->next) { + if (current_read->filtered) + continue; + aln_no_needed_consensus_bam_writer_.SaveAlignment(current_read->alignment); + } + pthread_mutex_unlock (&aln_no_needed_consensus_bam_writer_mutex_); + } + if (aln_needed_read_list != NULL){ + pthread_mutex_lock (&aln_needed_consensus_bam_writer_mutex_); + // Process linked list + for (Alignment *current_read = aln_needed_read_list; current_read; current_read = current_read->next) { + if (current_read->filtered) + continue; + aln_needed_consensus_bam_writer_.SaveAlignment(current_read->alignment); + } + pthread_mutex_unlock (&aln_needed_consensus_bam_writer_mutex_); + } +} + +// Generate the list of reads that cover the target +void ConsensusBAMWalkerEngine::BeginTargetProcessingTask(list::iterator& target_ticket){ + if (not tmp_begin_) + tmp_begin_ = alignments_first_; + long int target_start = next_target_->begin; + long int target_end = next_target_->end; + + while (tmp_begin_ and ( + (tmp_begin_->alignment.RefID == next_target_->chr and tmp_begin_->end <= target_start) + or tmp_begin_->alignment.RefID < next_target_->chr) + and tmp_begin_->processed) + tmp_begin_ = tmp_begin_->next; + + if (not tmp_end_) + tmp_end_ = tmp_begin_; + + while (tmp_end_ and ( + (tmp_end_->alignment.RefID == next_target_->chr and tmp_end_->original_position < target_end) + or tmp_end_->alignment.RefID < next_target_->chr) + and tmp_end_->processed) + tmp_end_ = tmp_end_->next; + + positions_in_progress_.push_back(PositionInProgress()); + target_ticket = positions_in_progress_.end(); + --target_ticket; + target_ticket->chr = next_target_->chr; + target_ticket->pos = next_position_; + target_ticket->target_begin = next_target_->begin; + target_ticket->target_end = next_target_->end; + target_ticket->begin = tmp_begin_; + target_ticket->end = tmp_end_; + target_ticket->start_time = time(NULL); + + + first_excess_read_ = tmp_end_->read_number; +} + +// Count how many reads that use the program for alignment. +void BAMWalkerEngine::AddReadToPG(Alignment *rai){ + string pg_of_read; + if (not rai->alignment.GetTag("PG", pg_of_read)) { + cerr << "ERROR: The PG tag is not present in read " << rai->alignment.Name << endl; + exit(1); + } + pair< map::iterator, bool> pg_finder; + pg_finder = read_counts_of_pg_.insert(pair(pg_of_read, 0)); + ++pg_finder.first->second; +} + +// return true if success, else false. +// Get the most popular PG that aligns the bam file, and it must be tmap. +bool BAMWalkerEngine::GetMostPopularTmap(SamProgram& most_popular_tmap){ + string most_popular_pg; + int current_popular_count = -1; + for (map::iterator it = read_counts_of_pg_.begin(); it != read_counts_of_pg_.end(); ++it){ + if ((int) it->second > current_popular_count){ + most_popular_pg = it->first; + } + } + if (most_popular_pg.substr(0, 4) != "tmap"){ + cerr << "ERROR: The most popular program (PG) that aligns the bam file is not tmap! See the header for more details." << endl; + for (map::iterator it = read_counts_of_pg_.begin(); it != read_counts_of_pg_.end(); ++it){ + cerr << " PG: "<< it->first << " , read count: "<< it->second << endl; + } + return false; + } + + for (SamProgramIterator I = bam_header_.Programs.Begin(); I != bam_header_.Programs.End(); ++I) { + if (I->HasID() and I->HasCommandLine()){ + if (I->ID == most_popular_pg){ + most_popular_tmap = *I; + return true; + } + } + } + return false; +} diff --git a/Analysis/VariantCaller/BAMWalkerEngine.h b/Analysis/VariantCaller/BAMWalkerEngine.h index cb7c6db4..5e7f30b7 100644 --- a/Analysis/VariantCaller/BAMWalkerEngine.h +++ b/Analysis/VariantCaller/BAMWalkerEngine.h @@ -49,7 +49,7 @@ struct Allele { // structure to encapsulate registered reads and alleles struct Alignment { - Alignment() { Reset(); } + Alignment() { Reset(); read_count = 1;} void Reset() { next = NULL; read_number = 0; @@ -67,10 +67,12 @@ struct Alignment { refmap_code.clear(); refmap_has_allele.clear(); refmap_allele.clear(); + target_coverage_indices.clear(); is_reverse_strand = false; measurements.clear(); measurements_length = 0; phase_params.clear(); + measurements_sd.clear(); runid.clear(); well_rowcol.clear(); read_bases.clear(); @@ -86,8 +88,9 @@ struct Alignment { flow_order_index = -1; read_group.clear(); prefix_bases.clear(); + suffix_bases.clear(); tag_info.Clear(); - read_count = 1; +// read_count = 1; } BamAlignment alignment; //! Raw BamTools alignment @@ -114,12 +117,14 @@ struct Alignment { vector refmap_code; vector refmap_has_allele; vector refmap_allele; + vector target_coverage_indices; // Candidate evaluator information bool is_reverse_strand; //! Indicates whether read is from the forward or reverse strand vector measurements; //! The measurement values for this read blown up to the length of the flow order int measurements_length;//! Original trimmed length of the ZM measurements vector vector phase_params; //! cf, ie, droop parameters of this read + vector measurements_sd; //! The standard deviation of measurements for consensus reads string runid; //! Identify the run from which this read came: used to find run-specific parameters vector well_rowcol; //! 2 element int vector 0-based row, col in that order mapping to row,col in chip string read_bases; //! Read sequence as base called (minus hard but including soft clips) @@ -135,6 +140,7 @@ struct Alignment { short flow_order_index; //! Index of the flow order belonging to this read string read_group; //! Read group of this read string prefix_bases; //! hard clipped start of the read + string suffix_bases; //! hard clipped end of the read // Post-processing information vector old_cigar; //! Cigar information before primer trimming @@ -146,6 +152,7 @@ struct PositionInProgress { int chr; //! Chromosome index of this variant position long pos; //! Position within chromosome + long target_begin; //! Begin of current target region long target_end; //! End of current target region Alignment * begin; //! First read covering this position Alignment * end; //! Last read coverint this position @@ -164,7 +171,7 @@ class BAMWalkerEngine { BAMWalkerEngine(); ~BAMWalkerEngine(); void Initialize(const ReferenceReader& ref_reader, TargetsManager& targets_manager, - const vector& bam_filenames, const string& postprocessed_bami, int px); + const vector& bam_filenames, const string& postprocessed_bam, int px); void Close(); const SamHeader& GetBamHeader() { return bam_header_; } @@ -174,7 +181,7 @@ class BAMWalkerEngine { bool ReadyForNextPosition(); // Memory contention prevention - bool MemoryContention(); + bool MemoryContention(int max_num_reads = 50000); bool IsEarlierstPositionProcessingTask(list::iterator& position_ticket); // Loading new reads @@ -203,47 +210,30 @@ class BAMWalkerEngine { basecaller_version = basecaller_version_; tmap_version = tmap_version_; } + void AddReadToPG(Alignment *rai); + bool GetMostPopularTmap(SamProgram& most_popular_tmap); + void processDepth(BamAlignment& alignment, TargetsManager* targets_manager, vector::iterator& curr_target); void openDepth(const string& filename); void writeDepth(ReferenceReader* reference_reader, std::map::size_type offset = 0); void closeDepth(ReferenceReader* reference_reader); + int getChrIndex() const {return next_target_->chr;} + long int getStartPosition() const {return next_target_->begin;} + long int getEndPosition() const {return next_target_->end;} + long int getPosition() const {return next_position_;} + Alignment * alignments_first_; //! First in a list of all alignments in memory private: void InitializeBAMs(const ReferenceReader& ref_reader, const vector& bam_filenames); TargetsManager * targets_manager_; //! Manages targets loaded from BED file - BamMultiReader bam_reader_; //! BamTools mulit-bam reader - SamHeader bam_header_; //! Bam header string basecaller_version_; //! BaseCaller version retrieved from BAM header string tmap_version_; //! TMAP version retrieved from BAM header - MergedTarget * next_target_; //! Target containing next position - long int next_position_; //! Next position (chr in the target) - - int last_processed_chr_; //! Reads up to this chr+pos are guaranteed to be processed - long int last_processed_pos_; //! Reads up to this chr+pos are guaranteed to be processed - bool has_more_alignments_; //! Are there still more reads in BAM? - bool has_more_positions_; //! Are there still more positions within the target region to process? -public: - Alignment * alignments_first_; //! First in a list of all alignments in memory -private: - Alignment * alignments_last_; //! Last in a list of all alignments in memory - int read_counter_; //! Total # of reads retrieved so far - Alignment * recycle_; //! Stack of allocated, reusable Alignment objects int recycle_size_; //! Size of the the recycle stack pthread_mutex_t recycle_mutex_; //! Mutex controlling access to the recycle stack - Alignment * tmp_begin_; //! Starts read window of most recent position task - Alignment * tmp_end_; //! Ends read window of most recent position task - - Alignment * processing_first_; //! First in a list of alignments being processed - Alignment * processing_last_; //! Last in a list of alignments being processed - list positions_in_progress_; //! List of positions being processed - int first_excess_read_; //! Index of the earliest read beyond the current position - - int first_useful_read_; //! Index of the earliest read that may still be in use - bool bam_writing_enabled_; BamWriter bam_writer_; @@ -260,8 +250,50 @@ class BAMWalkerEngine { int prevRefID; long int prevEndPos; +protected: + BamMultiReader bam_reader_; //! BamTools mulit-bam reader + SamHeader bam_header_; //! Bam header + + MergedTarget * next_target_; //! Target containing next position + long int next_position_; //! Next position (chr in the target) + + int last_processed_chr_; //! Reads up to this chr+pos are guaranteed to be processed + long int last_processed_pos_; //! Reads up to this chr+pos are guaranteed to be processed + bool has_more_alignments_; //! Are there still more reads in BAM? + bool has_more_positions_; //! Are there still more positions within the target region to process? + Alignment * alignments_last_; //! Last in a list of all alignments in memory + int read_counter_; //! Total # of reads retrieved so far + + Alignment * tmp_begin_; //! Starts read window of most recent position task + Alignment * tmp_end_; //! Ends read window of most recent position task + + Alignment * processing_first_; //! First in a list of alignments being processed + Alignment * processing_last_; //! Last in a list of alignments being processed + list positions_in_progress_; //! List of positions being processed + int first_excess_read_; //! Index of the earliest read beyond the current position + + int first_useful_read_; //! Index of the earliest read that may still be in use + map read_counts_of_pg_; //! Count how many reads that use the program for alignment. }; +class ConsensusBAMWalkerEngine : public BAMWalkerEngine +{ +private: + BamWriter aln_no_needed_consensus_bam_writer_; + BamWriter aln_needed_consensus_bam_writer_; + pthread_mutex_t aln_no_needed_consensus_bam_writer_mutex_; + pthread_mutex_t aln_needed_consensus_bam_writer_mutex_; + bool write_consensus_bam_ = true; +public: + ConsensusBAMWalkerEngine() : BAMWalkerEngine(){}; + void SaveConsensusAlignments(Alignment* const & read_list, Alignment* const & aln_needed_read_list); + void Initialize(const ReferenceReader& ref_reader, TargetsManager& targets_manager, + const vector& bam_filenames, const string& postprocessed_bam, int px, const string& consensus_bam); + void Close(); + void RequestTargetBasedReadRemovalTask(Alignment*& removal_list); + void BeginTargetProcessingTask(list::iterator& position_ticket); + bool EligibleForTargetBasedReadRemoval(); +}; #endif //BAMWALKERENGINE_H diff --git a/Analysis/VariantCaller/Bookkeeping/ExtendParameters.cpp b/Analysis/VariantCaller/Bookkeeping/ExtendParameters.cpp index ab8411fc..d1030656 100644 --- a/Analysis/VariantCaller/Bookkeeping/ExtendParameters.cpp +++ b/Analysis/VariantCaller/Bookkeeping/ExtendParameters.cpp @@ -1,7 +1,6 @@ /* Copyright (C) 2012 Ion Torrent Systems, Inc. All Rights Reserved */ #include "ExtendParameters.h" -#include #include #include #include @@ -18,13 +17,17 @@ void VariantCallerHelp() { printf("Usage: tvc [options]\n"); printf("\n"); + printf("Flow space consensus:\n"); + printf(" consensus generate flow space consensus reads by re-basecalling reads that are flow-synchronized\n"); + printf("\n"); + printf("General options:\n"); printf(" -h,--help print this help message and exit\n"); printf(" -v,--version print version and exit\n"); printf(" -n,--num-threads INT number of worker threads [2]\n"); printf(" -N,--num-variants-per-thread INT worker thread batch size [500]\n"); printf(" --parameters-file FILE json file with algorithm control parameters [optional]\n"); - printf(" --do-indel-assembly on/off do indel assembly to call long indel variants [on]\n"); + printf(" --do-indel-assembly on/off use indel assembler to call long indel variants [on]\n"); printf("\n"); printf("Inputs:\n"); @@ -34,17 +37,21 @@ void VariantCallerHelp() { printf(" --force-sample-name STRING force all read groups to have this sample name [off]\n"); printf(" -t,--target-file FILE only process targets in this bed file [optional]\n"); printf(" --trim-ampliseq-primers on/off match reads to targets and trim the ends that reach outside them [off]\n"); - printf(" -D,--downsample-to-coverage INT ?? [2000]\n"); + printf(" -D,--downsample-to-coverage INT downsample reads to this value for flow-space evaluation [2000]\n"); printf(" --model-file FILE HP recalibration model input file.\n"); printf(" --recal-model-hp-thres INT Lower threshold for HP recalibration.\n"); printf("\n"); printf("Outputs:\n"); printf(" -O,--output-dir DIRECTORY base directory for all output files [current dir]\n"); - printf(" -o,--output-vcf FILE vcf file with variant calling results [required]\n"); + printf(" -o,--output-vcf FILE vcf file with small variant calling results [small_variants.vcf]\n"); + printf(" --assembly-vcf FILE vcf file with indel assembly results [indel_assembly.vcf]\n"); + //printf(" --merged-vcf FILE merged and post processed vcf file [TSVC_variants.vcf]\n"); printf(" --suppress-reference-genotypes on/off write reference calls into the filtered variants vcf [on]\n"); printf(" --suppress-no-calls on/off write filtered variants into the filtered variants vcf [on]\n"); printf(" --suppress-nocall-genotypes on/off do not report a genotype for filtered variants [on]\n"); + printf(" --report-ppa on/off report Possible Polyploidy Alleles (PPA) [off]\n"); + printf(" --candidate-list-file FILE list all the novel candidates generated by freebayes [optional]\n"); printf("\n"); printf("Variant candidate generation (FreeBayes):\n"); @@ -54,17 +61,22 @@ void VariantCallerHelp() { printf(" --allow-complex on/off allow generation of block substitution candidates [off]\n"); printf(" --max-complex-gap INT maximum number of reference alleles between two alternate alleles to allow merging of the alternate alleles [1]\n"); printf(" -m,--use-best-n-alleles INT maximum number of snp alleles [2]\n"); + printf(" --use-best-n-nonsnp-alleles INT maximum number of non-snp alleles [0, no limit]\n"); printf(" -M,--min-mapping-qv INT do not use reads with mapping quality below this [4]\n"); printf(" -U,--read-snp-limit INT do not use reads with number of snps above this [10]\n"); + printf(" --read-mismatch-limit INT do not use reads with number of mismatches (where 1 gap open counts 1) above this value (0 to disable this option) [0]\n"); + printf(" --min-cov-fraction FLOAT do not use reads with fraction of covering the best assigned (unmerged) target region below this [0.0]\n"); printf(" -z,--read-max-mismatch-fraction FLOAT do not use reads with fraction of mismatches above this [1.0]\n"); printf(" --gen-min-alt-allele-freq FLOAT minimum required alt allele frequency to generate a candidate [0.2]\n"); - printf(" --gen-min-indel-alt-allele-freq FLOAT minimum required alt allele frequency to generate a homopolymer indel candidate [0.2]\n"); + printf(" --gen-min-indel-alt-allele-freq FLOAT minimum required alt allele frequency to generate an indel candidate [0.2]\n"); printf(" --gen-min-coverage INT minimum required coverage to generate a candidate [6]\n"); - printf(" --merge-variant-lookahead INT how many bases ahead to merge nearby variant to form correct haplotype [3, 0 if not allow complex]\n"); + printf(" --max-alt-num INT try to break the variant if the number of alt alleles is greater than this value. [20]\n"); + printf(" --merge-variant-lookahead INT how many bases ahead to merge nearby variant to form correct haplotype [-1 use auto-adapt, 0 if not allow complex]\n"); + printf(" --allele-cigar-output on/off output cigar for alt alleles [off]\n"); printf("\n"); printf("External variant candidates:\n"); - printf(" -l,--blacklist-vcf FILE vcf.gz file (+.tbi) with blacklist candidate variant locations and alleles [optional]\n"); + printf(" -l,--sse-vcf FILE vcf.gz file (+.tbi) with sse candidate variant locations and alleles [optional]\n"); printf(" -c,--input-vcf FILE vcf.gz file (+.tbi) with additional candidate variant locations and alleles [optional]\n"); printf(" --process-input-positions-only on/off only generate candidates at locations from input-vcf [off]\n"); printf(" --use-input-allele-only on/off only consider provided alleles for locations in input-vcf [off]\n"); @@ -74,7 +86,8 @@ void VariantCallerHelp() { printf(" --min-delta-for-flow FLOAT minimum prediction delta for scoring flows [0.1]\n"); printf(" --max-flows-to-test INT maximum number of scoring flows [10]\n"); printf(" --outlier-probability FLOAT probability for outlier reads [0.01]\n"); - printf(" --heavy-tailed INT degrees of freedom in t-dist modeling signal residual heavy tail [3]\n"); + printf(" --heavy-tailed INT (2*this value-1) is the Degrees of Freedom (DoF) in t-dist modeling signal residual heavy tail [3]\n"); + printf(" --adjust-sigma on/off It true, use sigma^2=(DoF-2)/Dof*VAR(residual) for calculating the t-dist log-likelihood, else use sigma^2=VAR(residual) [off]\n"); printf(" --suppress-recalibration on/off Suppress homopolymer recalibration [on].\n"); printf(" --do-snp-realignment on/off Realign reads in the vicinity of candidate snp variants [on].\n"); printf(" --do-mnp-realignment on/off Realign reads in the vicinity of candidate mnp variants [do-snp-realignment].\n"); @@ -93,22 +106,75 @@ void VariantCallerHelp() { printf(" --sse-relative-safety-level FLOAT dampen strand bias detection for SSE events for low coverage [0.025]\n"); printf(" --tune-sbias FLOAT dampen strand bias detection for low coverage [0.01]\n"); printf(" --max-detail-level INT number of evaluated frequencies for a given hypothesis, reduce for very high coverage, set to zero to disable this option [0]\n"); - printf(" --min-detail-level-for-fast-scan INT minimum detail level to trigger the fast scan for log-posterior of frequencies [2500]\n"); + printf(" --min-detail-level-for-fast-scan INT minimum detail level to trigger the fast scan for log-posterior of frequencies [0]\n"); printf(" --try-few-restart-freq on/off speed up the evaluator by trying fewer initial guesses of allele frequency [off].\n"); printf("\n"); printf("Variant filtering:\n"); // Filters depending on the variant type - printf(" -k,--snp-min-coverage INT filter out snps with total coverage below this [6]\n"); - printf(" -C,--snp-min-cov-each-strand INT filter out snps with coverage on either strand below this [0]\n"); - printf(" -B,--snp-min-variant-score FLOAT filter out snps with QUAL score below this [10.0]\n"); - printf(" -s,--snp-strand-bias FLOAT filter out snps with strand bias above this [0.95] given strand bias > snp-strand-bias\n"); + printf(" --use-fd-param on/off use flow-disruptiveness to choose parameter set. If on, map inputed (INDEL,SNP,MNP) to (fd-0,fd-5,fd-10) if fd parameters not specified [off]\n"); + printf(" --min-ratio-for-fd FLOAT claim flow-disruption if the portion of reads that are flow-disrupted >= this value [0.1]\n"); + printf(" --fd-nonsnp-min-var-cov INT override min-var-coverage of the flow-disrupted variants that are not snp (0 to disable the override) [0]\n"); + printf(" --hotspots-as-de-novo on/off treat hotspots as de novo during filtering [off]\n"); + // Filters not depending on the variant score + printf(" -L,--hp-max-length INT filter out indels in homopolymers above this [8]\n"); + printf(" --hp-indel-hrun INT VECTOR define the HRUN for filtering HP-INDEL variants [7,8]\n"); + printf(" --hp-ins-len INT VECTOR filter out HP-INS variants whose INS length <= the corresponding entry of this vector if the HRUN is defined in hp-indel-hrun [0,0]\n"); + printf(" --hp-del-len INT VECTOR filter out HP-DEL variants whose DEL length <= the corresponding entry of this vector if the HRUN is defined in hp-indel-hrun [0,0]\n"); + printf(" --error-motifs-dir FILE directory where the error-motifs files can be found [optional]\n"); + printf(" -e,--error-motifs FILE table of systematic error motifs and their error rates [optional]\n"); + printf(" --sse-prob-threshold FLOAT filter out variants in motifs with error rates above this [0.2]\n"); + printf(" --min-ratio-reads-non-sse-strand FLOAT minimum required alt allele frequency for variants with error motifs on opposite strand [0.2]\n"); + printf(" --use-lod-filter on/off enable the Limit Of Detection (LOD) filter [off]\n"); + printf(" --lod-multiplier FLOAT multiplier of LOD for filtering out variants with low alt allele frequency [0.6]\n"); + printf(" --tag-sim-max-cov INT check the similarity of variant molecular tags if the variant molecular coverage <= this value [20]\n"); + printf(" --cleanup-unlikely-candidates on/off remove unlikely variant candidates from the vcf records if there is a highly likely variant presented (similar to heal-snps) [on].\n"); + + printf("\nVariant filtering for hotspot alleles (default X = fd-10 if use-fd-param else X = snp):\n"); + printf(" --hotspot-min-allele-freq FLOAT minimum required alt allele frequency for hotspot variant call [X-min-allele-freq]\n"); + printf(" --hotspot-min-coverage INT filter out hotspot variants with total coverage below this [X-min-coverage]\n"); + printf(" --hotspot-min-cov-each-strand INT filter out hotspot variants with coverage on either strand below this [X-min-cov-each-strand]\n"); + printf(" --hotspot-min-var-coverage INT filter out hotspot variants with hard-classified variant allele coverage below this [X-min-cov-each-strand]\n"); + printf(" --hotspot-min-variant-score FLOAT filter out hotspot variants with QUAL score below this [X-min-variant-score]\n"); + printf(" --hotspot-strand-bias FLOAT filter out hotspot variants with strand bias above this [X-strand-bias] given strand bias > hotspot-strand-bias\n"); + printf(" --hotspot-strand-bias-pval FLOAT filter out hotspot variants with pval below this [X-strand-bias-pval] given pval < hotspot-strand-bias-pval\n"); + + printf("\nVariant filtering for fd-0 (non-flow-disrupted, i.e., HP-INDEL) alleles:\n"); + printf(" --fd-0-min-allele-freq FLOAT minimum required alt allele frequency for fd-0 variant call [0.2]\n"); + printf(" --fd-0-min-coverage INT filter out fd-0 variants with total coverage below this [30]\n"); + printf(" --fd-0-min-cov-each-strand INT filter out fd-0 variants with coverage on either strand below this [1]\n"); + printf(" --fd-0-min-var-coverage INT filter out fd-0 variants with hard-classified variant allele coverage below this [0]\n"); + printf(" --fd-0-min-variant-score FLOAT filter out fd-0 variants with QUAL score below this [10.0]\n"); + printf(" --fd-0-strand-bias FLOAT filter out fd-0 variants with strand bias above this [0.95] given strand bias > fd-0-strand-bias\n"); + printf(" --fd-0-strand-bias-pval FLOAT filter out fd-0 variants with pval below this [1.0] given pval < fd-0-strand-bias-pval\n"); + + printf("\nVariant filtering for fd-5 (moderate flow-disrupted) alleles:\n"); + printf(" --fd-5-min-allele-freq FLOAT minimum required alt allele frequency for fd-5 variant call [fd-0-min-allele-freq]\n"); + printf(" --fd-5-min-coverage INT filter out fd-5 variants with total coverage below this [fd-0-min-coverage]\n"); + printf(" --fd-5-min-cov-each-strand INT filter out fd-5 variants with coverage on either strand below this [fd-0-min-cov-each-strand]\n"); + printf(" --fd-5-min-var-coverage INT filter out fd-5 variants with hard-classified variant allele coverage below this [fd-0-min-var-coverage]\n"); + printf(" --fd-5-min-variant-score FLOAT filter out fd-5 variants with QUAL score below this [fd-0-min-variant-score]\n"); + printf(" --fd-5-strand-bias FLOAT filter out fd-5 variants with strand bias above this [fd-0-strand-bias] given strand bias > fd-5-strand-bias\n"); + printf(" --fd-5-strand-bias-pval FLOAT filter out fd-5 variants with pval below this [fd-0-strand-bias-pval] given pval < fd-5-strand-bias-pval\n"); + + printf("\nVariant filtering for fd-10 (most flow-disrupted) alleles:\n"); + printf(" --fd-10-min-allele-freq FLOAT minimum required alt allele frequency for fd-10 variant call [0.2]\n"); + printf(" --fd-10-min-coverage INT filter out fd-10 variants with total coverage below this [6]\n"); + printf(" --fd-10-min-cov-each-strand INT filter out fd-10 variants with coverage on either strand below this [0]\n"); + printf(" --fd-10-min-var-coverage INT filter out fd-10 variants with hard-classified variant allele coverage below this [0]\n"); + printf(" --fd-10-min-variant-score FLOAT filter out fd-10 variants with QUAL score below this [10.0]\n"); + printf(" --fd-10-strand-bias FLOAT filter out fd-10 variants with strand bias above this [0.95] given strand bias > fd-10-strand-bias\n"); + printf(" --fd-10-strand-bias-pval FLOAT filter out fd-10 variants with pval below this [1.0] given pval < fd-10-strand-bias-pval\n"); + + printf("\nParameters that will be deprecated in the future:\n"); + printf(" --snp-min-coverage INT filter out snps with total coverage below this [6]\n"); + printf(" --snp-min-cov-each-strand INT filter out snps with coverage on either strand below this [0]\n"); + printf(" --snp-min-variant-score FLOAT filter out snps with QUAL score below this [10.0]\n"); + printf(" --snp-strand-bias FLOAT filter out snps with strand bias above this [0.95] given strand bias > snp-strand-bias\n"); printf(" --snp-strand-bias-pval FLOAT filter out snps with pval below this [1.0] given pval < snp-strand-bias-pval\n"); - // printf(" -s,--snp-strand-bias FLOAT filter out snps with strand bias above this [0.95]\n"); - printf(" -A,--snp-min-allele-freq FLOAT minimum required alt allele frequency for non-reference snp calls [0.2]\n"); + printf(" --snp-min-allele-freq FLOAT minimum required alt allele frequency for non-reference snp calls [0.2]\n"); printf(" --snp-min-var-coverage INT filter out snps with variant allele coverage below this [0]\n"); - printf(" --mnp-min-coverage INT filter out mnps with total coverage below this [snp-min-coverage]\n"); printf(" --mnp-min-cov-each-strand INT filter out mnps with coverage on either strand below this, [snp-min-cov-each-strand]\n"); printf(" --mnp-min-variant-score FLOAT filter out mnps with QUAL score below this [snp-min-variant-score]\n"); @@ -116,33 +182,15 @@ void VariantCallerHelp() { printf(" --mnp-strand-bias-pval FLOAT filter out mnps with pval below this [snp-strand-bias-pval] given pval < mnp-strand-bias-pval\n"); printf(" --mnp-min-allele-freq FLOAT minimum required alt allele frequency for non-reference mnp calls [snp-min-allele-freq]\n"); printf(" --mnp-min-var-coverage INT filter out mnps with variant allele coverage below this [snp-min-var-coverage]\n"); - printf(" --indel-min-coverage INT filter out indels with total coverage below this [30]\n"); printf(" --indel-min-cov-each-strand INT filter out indels with coverage on either strand below this [1]\n"); printf(" --indel-min-variant-score FLOAT filter out indels with QUAL score below this [10.0]\n"); - printf(" -S,--indel-strand-bias FLOAT filter out indels with strand bias above this [0.95] given strand bias > indel-strand-bias\n"); + printf(" --indel-strand-bias FLOAT filter out indels with strand bias above this [0.95] given strand bias > indel-strand-bias\n"); printf(" --indel-strand-bias-pval FLOAT filter out indels with pval below this [1.0] given pval < indel-strand-bias-pval\n"); - // printf(" -S,--indel-strand-bias FLOAT filter out indels with strand bias above this [0.85]\n"); printf(" --indel-min-allele-freq FLOAT minimum required alt allele frequency for non-reference indel call [0.2]\n"); printf(" --indel-min-var-coverage INT filter out indels with variant allele coverage below this [snp-min-var-coverage]\n"); - - printf(" --hotspot-min-coverage INT filter out hotspot variants with total coverage below this [6]\n"); - printf(" --hotspot-min-cov-each-strand INT filter out hotspot variants with coverage on either strand below this [snp-min-cov-each-strand]\n"); - printf(" --hotspot-min-variant-score FLOAT filter out hotspot variants with QUAL score below this [snp-min-variant-score]\n"); - printf(" --hotspot-strand-bias FLOAT filter out hotspot variants with strand bias above this [0.95] given strand bias > hotspot-strand-bias\n"); - printf(" --hotspot-strand-bias-pval FLOAT filter out hotspot variants with pval below this [1.0] given pval < hotspot-strand-bias-pval\n"); - // printf(" --hotspot-strand-bias FLOAT filter out hotspot variants with strand bias above this [0.95]\n"); - printf(" -H,--hotspot-min-allele-freq FLOAT minimum required alt allele frequency for non-reference hotspot variant call [0.2]\n"); - printf(" --hotspot-min-var-coverage INT filter out hotspot variants with variant allele coverage below this [snp-min-var-coverage]\n"); - - // Filters not depending on the variant score - printf(" -L,--hp-max-length INT filter out indels in homopolymers above this [8]\n"); - printf(" -e,--error-motifs FILE table of systematic error motifs and their error rates [optional]\n"); - printf(" --sse-prob-threshold FLOAT filter out variants in motifs with error rates above this [0.2]\n"); - printf(" --min-ratio-reads-non-sse-strand FLOAT minimum required alt allele frequency for variants with error motifs on opposite strand [0.2]\n"); printf(" --indel-as-hpindel on/off apply indel filters to non HP indels [off]\n"); - printf(" --use-lod-filter on/off enable the Limit Of Detection (LOD) filter [off]\n"); - printf(" --lod-multiplier FLOAT multiplier of LOD for filtering out variants with low alt allele frequency [0.6]\n"); + printf(" --heal-snps on/off suppress in/dels not participating in diploid variant genotypes if the genotype contains a SNP or MNP [on].\n"); // position-bias filter printf("\nPosition bias variant filtering:\n"); @@ -157,26 +205,51 @@ void VariantCallerHelp() { printf(" --filter-unusual-predictions FLOAT posterior log likelihood threshold for accepting bias estimate [0.3]\n"); printf(" --filter-deletion-predictions FLOAT check post-evaluation systematic bias in deletions; a high value like 100 effectively turns off this filter [100.0]\n"); printf(" --filter-insertion-predictions FLOAT check post-evaluation systematic bias in insertions; a high value like 100 effectively turns off this filter [100.0]\n"); - printf("\n"); - printf(" --heal-snps on/off suppress in/dels not participating in diploid variant genotypes if the genotype contains a SNP or MNP [on].\n"); + // XXX Not applicable in TS 5.4 since merging and post processing is not run in TVC + //printf("\nVCF record filters (applied during merge):\n"); + //printf(" --filter-by-target on/off Filter vcf records by meta information in the target bed file [on]\n"); + //printf(" --hotspot-positions-only on/off Report only hotspot vcf records in final output [off]\n"); + //printf(" --hotspot-variants-only on/off Suppress hotspot records that are no-calls or reference-calls [off]\n"); printf("\n"); MolecularTagTrimmer::PrintHelp(true); + printf(" --indel-func-size-offset INT require family of size >= (min-tag-fam-size + this value) to be functional when calling HP-INDEL [0]\n"); + printf("\n"); + printf("Debugging:\n"); - printf(" -d,--debug INT (0/1/2) display extra debug messages [0]\n"); + printf(" -d,--debug INT (0/1/2/3) display extra debug messages [0]\n"); printf(" --do-json-diagnostic on/off (devel) dump internal state to json file (uses much more time/memory/disk) [off]\n"); printf(" --postprocessed-bam FILE (devel) save tvc-processed reads to an (unsorted) BAM file [optional]\n"); printf(" --do-minimal-diagnostic on/off (devel) provide minimal read information for called variants [off]\n"); printf(" --override-limits on/off (devel) disable limit-check on input parameters [off].\n"); + printf(" --disable-filters on/off (devel) disable most of the filtering steps for the alleles [off].\n"); printf(" --output-multi-min-allele-freq on/off output the inference results for multiple min-allele-freq in vcf [off].\n"); - printf(" --snp-multi-min-allele-freq FLOAT VECTOR multiple min-allele-freq for snp calls [0.05,0.1,0.15,0.2].\n"); - printf(" --mnp-multi-min-allele-freq FLOAT VECTOR multiple min-allele-freq for mnp calls [snp-multi-min-allele-freq].\n"); - printf(" --indel-multi-min-allele-freq FLOAT VECTOR multiple min-allele-freq for indel calls [0.05,0.1,0.15,0.2].\n"); - printf(" --hotspot-multi-min-allele-freq FLOAT VECTOR multiple min-allele-freq for hotspot calls [0.05,0.1,0.15,0.2].\n"); + printf(" --multi-min-allele-freq FLOAT VECTOR output QUAL, GT, GQ using the min-allele-freq specified in the vector [0.05,0.1,0.15,0.2].\n"); printf("\n"); } +int mkpath(std::string s,mode_t mode) +{ + size_t pre=0,pos; + std::string dir; + int mdret = 0; + + if(s[s.size()-1]!='/'){ + // force trailing / so we can handle everything in loop + s+='/'; + } + + while((pos=s.find_first_of('/',pre))!=std::string::npos){ + dir=s.substr(0,pos++); + pre=pos; + if(dir.size()==0) continue; // if leading / first time is 0 length + if((mdret=mkdir(dir.c_str(),mode)) && errno!=EEXIST){ + return mdret; + } + } + return mdret; +} ControlCallAndFilters::ControlCallAndFilters() { // all defaults handled by sub-filters @@ -191,6 +264,13 @@ ControlCallAndFilters::ControlCallAndFilters() { use_lod_filter = false; lod_multiplier = 0.6f; + tag_sim_max_cov = 20; + + // VCF record filters (applied during vcf merging) XXX + //filter_by_target = true; // Filter records based on mets information in target bed info field + //hotspot_positions_only = false; // Output only vcf lines with the infoFlag 'HS' + //hotspot_variants_only = false; // Suppress hotspot reference calls and no-calls from the final output vcf + //xbias_tune = 0.005f; sbias_tune = 0.5f; downSampleCoverage = 2000; @@ -199,7 +279,15 @@ ControlCallAndFilters::ControlCallAndFilters() { suppress_reference_genotypes = true; suppress_nocall_genotypes = true; suppress_no_calls = true; - heal_snps = true; + cleanup_unlikely_candidates = true; + report_ppa = false; + hotspots_as_de_novo = false; + disable_filters = false; + + // Flow-disruption parameters + fd_nonsnp_min_var_cov = 0; + use_fd_param = false; + min_ratio_for_fd = 0.1f; } ProgramControlSettings::ProgramControlSettings() { @@ -207,7 +295,6 @@ ProgramControlSettings::ProgramControlSettings() { nThreads = 1; DEBUG = 0; do_indel_assembly = true; - #ifdef __SSE3__ use_SSE_basecaller = true; #else @@ -215,313 +302,14 @@ ProgramControlSettings::ProgramControlSettings() { #endif rich_json_diagnostic = false; minimal_diagnostic = false; - json_plot_dir = "./json_diagnostic/"; inputPositionsOnly = false; suppress_recalibration = true; resolve_clipped_bases = false; is_multi_min_allele_freq = false; - snp_multi_min_allele_freq.clear(); - mnp_multi_min_allele_freq.clear(); - indel_multi_min_allele_freq.clear(); - hotspot_multi_min_allele_freq.clear(); -} - - -int GetParamsInt(Json::Value& json, const string& key, int default_value) { - if (not json.isMember(key)) - return default_value; - if (json[key].isString()) - return atoi(json[key].asCString()); - return json[key].asInt(); -} - -double GetParamsDbl(Json::Value& json, const string& key, double default_value) { - if (not json.isMember(key)) - return default_value; - if (json[key].isString()) - return atof(json[key].asCString()); - return json[key].asDouble(); + multi_min_allele_freq.clear(); } - -int RetrieveParameterInt(OptArgs &opts, Json::Value& json, char short_name, const string& long_name_hyphens, int default_value) -{ - string long_name_underscores = long_name_hyphens; - for (unsigned int i = 0; i < long_name_underscores.size(); ++i) - if (long_name_underscores[i] == '-') - long_name_underscores[i] = '_'; - - int value = default_value; - string source = "builtin default"; - - if (json.isMember(long_name_underscores)) { - if (json[long_name_underscores].isString()) - value = atoi(json[long_name_underscores].asCString()); - else - value = json[long_name_underscores].asInt(); - source = "parameters json file"; - } - - if (opts.HasOption(short_name, long_name_hyphens)) { - value = opts.GetFirstInt(short_name, long_name_hyphens, value); - source = "command line option"; - } - - cout << setw(35) << long_name_hyphens << " = " << setw(10) << value << " (integer, " << source << ")" << endl; - return value; -} - -double RetrieveParameterDouble(OptArgs &opts, Json::Value& json, char short_name, const string& long_name_hyphens, double default_value) -{ - string long_name_underscores = long_name_hyphens; - for (unsigned int i = 0; i < long_name_underscores.size(); ++i) - if (long_name_underscores[i] == '-') - long_name_underscores[i] = '_'; - - double value = default_value; - string source = "builtin default"; - - if (json.isMember(long_name_underscores)) { - if (json[long_name_underscores].isString()) - value = atof(json[long_name_underscores].asCString()); - else - value = json[long_name_underscores].asDouble(); - source = "parameters json file"; - } - - if (opts.HasOption(short_name, long_name_hyphens)) { - value = opts.GetFirstDouble(short_name, long_name_hyphens, value); - source = "command line option"; - } - - cout << setw(35) << long_name_hyphens << " = " << setw(10) << value << " (double, " << source << ")" << endl; - return value; -} - - -bool RetrieveParameterBool(OptArgs &opts, Json::Value& json, char short_name, const string& long_name_hyphens, bool default_value) -{ - string long_name_underscores = long_name_hyphens; - for (unsigned int i = 0; i < long_name_underscores.size(); ++i) - if (long_name_underscores[i] == '-') - long_name_underscores[i] = '_'; - - bool value = default_value; - string source = "builtin default"; - - if (json.isMember(long_name_underscores)) { - if (json[long_name_underscores].isString()) - value = atoi(json[long_name_underscores].asCString()); - else - value = json[long_name_underscores].asInt(); - source = "parameters json file"; - } - - if (opts.HasOption(short_name, long_name_hyphens)) { - value = opts.GetFirstBoolean(short_name, long_name_hyphens, value); - source = "command line option"; - } - - cout << setw(35) << long_name_hyphens << " = " << setw(10) << (value ? "true" : "false") << " (boolean, " << source << ")" << endl; - return value; -} - -void ConvertToUpper(string &s){ - for(unsigned int i = 0; i -1) - { - s = name.substr(index + 2, name.length() - index - 2); - } - - for (unsigned int i = 0; i < s.size(); ++i) - { - if (s[i] == '-') - s[i] = '_'; - } - - return s; -} - -void Split(const string& s, char c, vector& v) -{ - v.clear(); - if (s != "") { - string::size_type i = 0; - string::size_type j = s.find (c); - if (j == string::npos) - { - v.push_back (s); - } - else - { - while (j != string::npos) - { - v.push_back (s.substr (i, j-i)); - i = ++j; - j = s.find (c,j); - - if (j == string::npos) - { - v.push_back (s.substr (i, s.length())); - } - } - } - } -} - -int RetrieveParameterVectorFloat(OptArgs &opts, Json::Value& json, char short_name, const string& long_name_hyphens, const string& default_value, vector& ret_vector) -{ - string long_name_underscores = GetRidOfDomainAndHyphens(long_name_hyphens); - string value = default_value; - - if(value.length() > 0) - { - vector words; - Split(value,',',words); - ret_vector.clear(); - for (size_t i = 0; i < words.size(); i++) { - char *end; - int err = errno; - errno = 0; - ret_vector.push_back(strtod(words[i].c_str(), &end)); - if (errno != 0 || *end != '\0') { - cout << "Error converting: " + words[i] + " to an float for option: " + long_name_hyphens << endl; - return errno; - } - errno = err; - } - } - string source = "builtin default"; - - if (json.isMember(long_name_underscores)) { - ret_vector.clear(); - size_t sz = json[long_name_underscores].size(); - char buf[1000]; - if(sz > 0) - { - if(sz == 1) - { - if(json[long_name_underscores][0].isString()) - { - ret_vector.push_back(atof(json[long_name_underscores][0].asCString())); - value = json[long_name_underscores][0].asCString(); - } - else - { - ret_vector.push_back(json[long_name_underscores][0].asFloat()); - sprintf(buf, "%f", ret_vector[0]); - value = buf; - } - } - else - { - value = ""; - for(int i = 0; i < (int)sz - 1; i++) - { - if(json[long_name_underscores][i].isString()) - { - ret_vector.push_back(atof(json[long_name_underscores][i].asCString())); - value += json[long_name_underscores][i].asCString(); - value += ","; - } - else - { - ret_vector.push_back(json[long_name_underscores][i].asFloat()); - sprintf(buf, "%f,", ret_vector[i]); - string ss = buf; - value += ss; - } - } - - if(json[long_name_underscores][(int)sz - 1].isString()) - { - ret_vector.push_back(atof(json[long_name_underscores][(int)sz - 1].asCString())); - value += json[long_name_underscores][(int)sz - 1].asCString(); - } - else - { - ret_vector.push_back(json[long_name_underscores][(int)sz - 1].asFloat()); - sprintf(buf, "%f", ret_vector[(int)sz - 1]); - string ss = buf; - value += ss; - } - } - source = "parameters json file"; - } - } - - if (opts.HasOption(short_name, long_name_hyphens)) { - ret_vector.clear(); - vector ret_vector2; - opts.GetOption(ret_vector2, default_value, short_name, long_name_hyphens); - for(size_t i = 0; i < ret_vector2.size(); i++) - { - ret_vector.push_back((float)ret_vector2[i]); - } - - char buf[1000]; - if(ret_vector.empty()) - { - cout << "Error setting: there is no value set for option: " + long_name_hyphens << endl; - return 1; - } - else if(ret_vector.size() == 1) - { - sprintf(buf, "%f", ret_vector[0]); - value = buf; - } - else - { - value = ""; - for(size_t i = 0; i < ret_vector.size() - 1; i++) { - sprintf(buf, "%f,", ret_vector[i]); - string ss = buf; - value += ss; - } - sprintf(buf, "%f", ret_vector[ret_vector.size() - 1]); - string ss = buf; - value += ss; - } - source = "command line option"; - } - - cout << setw(35) << long_name_hyphens << " = " << setw(10) << value << " (float, " << source << ")" << endl; - return 0; -} - - // ============================================================================= void EnsembleEvalTuningParameters::SetOpts(OptArgs &opts, Json::Value& tvc_params) { @@ -533,13 +321,14 @@ void EnsembleEvalTuningParameters::SetOpts(OptArgs &opts, Json::Value& tvc_param outlier_prob = RetrieveParameterDouble(opts, tvc_params, '-', "outlier-probability", 0.01); germline_prior_strength = RetrieveParameterDouble(opts, tvc_params, '-', "germline-prior-strength", 0.0f); heavy_tailed = RetrieveParameterInt (opts, tvc_params, '-', "heavy-tailed", 3); - + adjust_sigma = RetrieveParameterBool (opts, tvc_params, '-', "adjust-sigma", false); + filter_unusual_predictions = RetrieveParameterDouble(opts, tvc_params, '-', "filter-unusual-predictions", 0.3f); soft_clip_bias_checker = RetrieveParameterDouble(opts, tvc_params, '-', "soft-clip-bias-checker", 0.1f); filter_deletion_bias = RetrieveParameterDouble(opts, tvc_params, '-', "filter-deletion-predictions", 100.0f); filter_insertion_bias = RetrieveParameterDouble(opts, tvc_params, '-', "filter-insertion-predictions", 100.0f); max_detail_level = RetrieveParameterInt(opts, tvc_params, '-', "max-detail-level", 0); - min_detail_level_for_fast_scan = RetrieveParameterInt(opts, tvc_params, '-', "min-detail-level-for-fast-scan", 2500); + min_detail_level_for_fast_scan = RetrieveParameterInt(opts, tvc_params, '-', "min-detail-level-for-fast-scan", 0); try_few_restart_freq = RetrieveParameterBool(opts, tvc_params, '-', "try-few-restart-freq", false); // shouldn't majorly affect anything, but still expose parameters for completeness @@ -576,6 +365,54 @@ void EnsembleEvalTuningParameters::CheckParameterLimits() { // ============================================================================ +// I really don't want to do this. +void RetrieveParameterStringtifiedVectorIntForIR(Json::Value& json, const string& long_name_hyphens, vector& ret_vector){ + string long_name_underscores = long_name_hyphens; + for (unsigned int idx = 0; idx < long_name_underscores.size(); ++idx){ + if (long_name_underscores[idx] == '-'){ + long_name_underscores[idx] = '_'; + } + } + if (not json.isMember(long_name_underscores)){ + return; + } + if (not json[long_name_underscores].isString()){ + return; + } + string my_value = json[long_name_underscores].toStyledString(); + string my_vector_to_print; + my_vector_to_print.reserve(my_value.size()); + for (string::iterator my_it = my_value.begin(); my_it != my_value.end(); ++my_it){ + // Note that the string returned from toStyledString() starts with "\"" and ends with "\"\n". + if (*my_it != '\n' and *my_it != ' ' and *my_it != '"' and *my_it != '\t'){ + my_vector_to_print.push_back(*my_it); + } + } + + if (not (*(my_vector_to_print.begin()) == '[' and my_vector_to_print.back() == ']')){ + cerr << "WARNING: Skip the invalid value of the json parameter \"" << long_name_underscores << "\": \""<< my_vector_to_print << "\"" << endl; + return; + } + + ret_vector.resize(0); + ret_vector.reserve(my_vector_to_print.size() / 2); + my_value = my_vector_to_print; + + size_t comma_idx = (my_vector_to_print == "[]") ? string::npos : 0; + while (comma_idx != string::npos and my_value.size() > 1){ + my_value.erase(0, comma_idx + 1); + int my_entry = (int) strtol(my_value.c_str(), NULL, 0); + if (my_entry == 0 and my_value[0] != '0'){ + cerr << "WARNING: Skip the invalid value of the json parameter \"" << long_name_underscores << "\": \""<< my_vector_to_print << "\"" << endl; + ret_vector.clear(); + return; + } + ret_vector.push_back(my_entry); + comma_idx = my_value.find(","); + } + cout << setw(35) << long_name_hyphens << " = " << setw(10) << my_vector_to_print << " (vector, parameters json file (specified as a string))" << endl; +} + void ClassifyFilters::SetOpts(OptArgs &opts, Json::Value & tvc_params) { hp_max_length = RetrieveParameterInt (opts, tvc_params, 'L', "hp-max-length", 8); @@ -587,68 +424,198 @@ void ClassifyFilters::SetOpts(OptArgs &opts, Json::Value & tvc_params) { do_mnp_realignment = RetrieveParameterBool (opts, tvc_params, '-', "do-mnp-realignment", do_snp_realignment); realignment_threshold = RetrieveParameterDouble(opts, tvc_params, '-', "realignment-threshold", 1.0); - indel_as_hpindel = RetrieveParameterBool (opts, tvc_params, '-', "indel-as-hpindel", false); + indel_as_hpindel = RetrieveParameterBool (opts, tvc_params, '-', "indel-as-hpindel", false); + + RetrieveParameterVectorInt(opts, tvc_params, '-', "hp-indel-hrun", "-1,-1", filter_hp_indel_hrun); + // Deal with the IR "stringtified" format. + if (filter_hp_indel_hrun == vector({-1, -1})){ + RetrieveParameterStringtifiedVectorIntForIR(tvc_params, "hp-indel-hrun", filter_hp_indel_hrun); + } + if (filter_hp_indel_hrun == vector({-1, -1})){ + filter_hp_indel_hrun = {7, 8}; + cout <<" hp-indel-hrun = 7,8 (int, builtin default)" << endl; + } + + RetrieveParameterVectorInt(opts, tvc_params, '-', "hp-ins-len", "-1,-1", filter_hp_ins_len); + // Deal with the IR "stringtified" format. + if (filter_hp_ins_len == vector({-1, -1})){ + RetrieveParameterStringtifiedVectorIntForIR(tvc_params, "hp-ins-len", filter_hp_ins_len); + } + if (filter_hp_ins_len == vector({-1, -1})){ + filter_hp_ins_len = {0, 0}; + cout << " hp-ins-len = 0,0 (int, builtin default)" << endl; + } + + RetrieveParameterVectorInt(opts, tvc_params, '-', "hp-del-len", "-1,-1", filter_hp_del_len); + // Deal with the IR "stringtified" format. + if (filter_hp_del_len == vector({-1, -1})){ + RetrieveParameterStringtifiedVectorIntForIR(tvc_params, "hp-del-len", filter_hp_del_len); + } + if (filter_hp_del_len == vector({-1, -1})){ + filter_hp_del_len = {0, 0}; + cout << " hp-del-len = 0,0 (int, builtin default)" << endl; + } } void ClassifyFilters::CheckParameterLimits() { - CheckParameterLowerBound ("hp-max-length", hp_max_length, 1); - CheckParameterLowerUpperBound("sse-prob-threshold", sseProbThreshold, 0.0f, 1.0f); + CheckParameterLowerBound ("hp-max-length", hp_max_length, 1); + CheckParameterLowerUpperBound("sse-prob-threshold", sseProbThreshold, 0.0f, 1.0f); CheckParameterLowerUpperBound("min-ratio-reads-non-sse-strand", minRatioReadsOnNonErrorStrand, 0.0f, 1.0f); CheckParameterLowerUpperBound("sse-relative-safety-level", sse_relative_safety_level, 0.0f, 1.0f); CheckParameterLowerUpperBound("realignment-threshold", realignment_threshold, 0.0f, 1.0f); + cout << "Size check parameters hp-indel-hrun, hp-ins-len, hp-del-len: hp_indel_hrun.size()==hp_ins_len.size()==hp_del_len.size()? "; + if (filter_hp_indel_hrun.size() == filter_hp_ins_len.size() and filter_hp_indel_hrun.size() == filter_hp_del_len.size()){ + cout << "OK!" << endl; + }else{ + cout << "Using " << "hp_indel_hrun = {7,8}, filter_hp_ins_len = {0,0}, filter_hp_del_len = {0,0} instead!" << endl; + filter_hp_indel_hrun = {7, 8}; + filter_hp_ins_len = {0, 0}; + filter_hp_del_len = {0, 0}; + } + + for (unsigned int i_hp = 0; i_hp < filter_hp_indel_hrun.size(); ++i_hp){ + string identifier = "hp-indel-hrun[" + convertToString(i_hp) + "]"; + CheckParameterLowerBound (identifier, filter_hp_indel_hrun[i_hp], 0); + } + for (unsigned int i_hp = 0; i_hp < filter_hp_ins_len.size(); ++i_hp){ + string identifier = "hp-ins-len[" + convertToString(i_hp) + "]"; + CheckParameterLowerBound (identifier, filter_hp_ins_len[i_hp], 0); + } + for (unsigned int i_hp = 0; i_hp < filter_hp_del_len.size(); ++i_hp){ + string identifier = "hp-del-len[" + convertToString(i_hp) + "]"; + CheckParameterLowerBound (identifier, filter_hp_del_len[i_hp], 0); + } +} + +BasicFilters::BasicFilters(const string& filter_type) { + my_type = filter_type; + underscored_my_type = my_type; + for (string::iterator s_it = underscored_my_type.begin(); s_it != underscored_my_type.end(); ++s_it){ + if (*s_it == '-'){ + *s_it = '_'; + } + } + if (underscored_my_type == "snp" or underscored_my_type == "fd_10"){ + min_allele_freq = 0.2f; + strand_bias_threshold = 0.95f; + strand_bias_pval_threshold = 1.0f; + min_quality_score = 10.0f; + min_cov = 6; + min_cov_each_strand = 0; + min_var_cov = 0; + } + else if (underscored_my_type == "indel" or underscored_my_type == "fd_0"){ + min_allele_freq = 0.2f; + strand_bias_threshold = 0.95f; + strand_bias_pval_threshold = 1.0f; + min_quality_score = 10.0f; + min_cov = 30; + min_cov_each_strand = 1; + min_var_cov = 0; + } +} +void BasicFilters::SetBasicFilterOpts(OptArgs &opts, Json::Value& tvc_params, BasicFilters* default_basic_filter){ + string opt_prefix; + Json::Value tvc_params_section; + // I will get the X parameters from the kTypeMap[X] parameters in the "torrent_variant_caller" section + const static map kTypeMap = {pair("fd-10", "mnp"), + pair("fd-5", "snp"), + pair("fd-0", "indel"), + pair("hotspot", "hotspot"), + pair("mnp", "mnp"), + pair("snp", "snp"), + pair("indel", "indel")}; + + map::const_iterator map_it = kTypeMap.find(my_type); + if (map_it == kTypeMap.end()){ + cerr << "ERROR: Unkown BasicFilter type: " << my_type << endl; + exit(-1); + } + // First get parameter from the "torrent_variant_caller" section + opt_prefix = map_it->second + "-"; + if (map_it->second != my_type){ + cout << endl << "Retrieving the " << map_it->second << " parameters that will be propagate to the " << my_type << " parameters:" << endl; + } + + if (default_basic_filter == NULL){ + default_basic_filter = this; + } + + min_cov_each_strand = RetrieveParameterInt (opts, tvc_params, '-', opt_prefix + "min-cov-each-strand", default_basic_filter->min_cov_each_strand); + min_quality_score = RetrieveParameterDouble(opts, tvc_params, '-', opt_prefix + "min-variant-score", default_basic_filter->min_quality_score); + min_allele_freq = RetrieveParameterDouble(opts, tvc_params, '-', opt_prefix + "min-allele-freq", default_basic_filter->min_allele_freq); + min_cov = RetrieveParameterInt (opts, tvc_params, '-', opt_prefix + "min-coverage", default_basic_filter->min_cov); + min_var_cov = RetrieveParameterInt (opts, tvc_params, '-', opt_prefix + "min-var-coverage", default_basic_filter->min_var_cov); + strand_bias_threshold = RetrieveParameterDouble(opts, tvc_params, '-', opt_prefix + "strand-bias", default_basic_filter->strand_bias_threshold); + strand_bias_pval_threshold = RetrieveParameterDouble(opts, tvc_params, '-', opt_prefix + "strand-bias-pval", default_basic_filter->strand_bias_pval_threshold); + if (map_it->second != my_type){ + cout << "Translating the above " << map_it->second << " parameters to the " << my_type << " parameters." << endl; + } + + // Then get parameter from the subsection in "torrent_variant_caller" + opt_prefix = my_type + "-"; + if (tvc_params.isMember(underscored_my_type)){ + string underscored_opt_prefix = underscored_my_type + "_"; + tvc_params_section = tvc_params[underscored_my_type]; + vector keys = tvc_params_section.getMemberNames(); + for (vector::iterator key_it = keys.begin(); key_it != keys.end(); ++key_it){ + // In case some one have something like this "fd_0": {"fd_0_min_allele_freq": 0.1,} while I suppose to see something like this "fd_0": {"min_allele_freq": 0.1,} + tvc_params_section.removeMember(underscored_opt_prefix + *key_it); + tvc_params_section[underscored_opt_prefix + *key_it] = tvc_params_section[*key_it]; + } + } + + cout << "Retrieving the " << my_type << " parameters from the command line and parameter_json[\"torrent_variant_caller\"][\""<< underscored_my_type << "\"]:" << endl; + min_cov_each_strand = RetrieveParameterInt (opts, tvc_params_section, '-', opt_prefix + "min-cov-each-strand", min_cov_each_strand); + min_quality_score = RetrieveParameterDouble(opts, tvc_params_section, '-', opt_prefix + "min-variant-score", min_quality_score); + min_allele_freq = RetrieveParameterDouble(opts, tvc_params_section, '-', opt_prefix + "min-allele-freq", min_allele_freq); + min_cov = RetrieveParameterInt (opts, tvc_params_section, '-', opt_prefix + "min-coverage", min_cov); + min_var_cov = RetrieveParameterInt (opts, tvc_params_section, '-', opt_prefix + "min-var-coverage", min_var_cov); + strand_bias_threshold = RetrieveParameterDouble(opts, tvc_params_section, '-', opt_prefix + "strand-bias", strand_bias_threshold); + strand_bias_pval_threshold = RetrieveParameterDouble(opts, tvc_params_section, '-', opt_prefix + "strand-bias-pval", strand_bias_pval_threshold); + cout << "(Note): Here, \"builtin default\" may be referred to as the the parameter has been set previously." << endl + << "Retrieving " << my_type << " parameter set complete." << endl; + +} + + +void BasicFilters::CheckBasicFiltersLimits(){ + string opt_prefix = my_type + "-"; + CheckParameterLowerBound (opt_prefix + "min-cov-each-strand", min_cov_each_strand, 0); + CheckParameterLowerBound (opt_prefix + "min-variant-score", min_quality_score, 0.0f); + CheckParameterLowerUpperBound(opt_prefix + "min-allele-freq", min_allele_freq, 0.0f, 1.0f); + CheckParameterLowerBound (opt_prefix + "min-coverage", min_cov, 0); + CheckParameterLowerUpperBound(opt_prefix + "strand-bias", strand_bias_threshold, 0.5f, 1.0f); + CheckParameterLowerUpperBound(opt_prefix + "strand-bias-pval", strand_bias_pval_threshold, 0.0f, 1.0f); + CheckParameterLowerBound (opt_prefix + "min-var-coverage", min_var_cov, 0); } // =========================================================================== void ControlCallAndFilters::CheckParameterLimits() { - filter_variant.CheckParameterLimits(); - CheckParameterLowerBound ("data-quality-stringency", data_quality_stringency, 0.0f); - CheckParameterLowerUpperBound("read-rejection-threshold", read_rejection_threshold, 0.0f, 1.0f); - CheckParameterLowerUpperBound ("downsample-to-coverage", downSampleCoverage, 20, 100000); - CheckParameterLowerUpperBound("position-bias-ref-fraction",position_bias_ref_fraction, 0.0f, 1.0f); - CheckParameterLowerUpperBound("position-bias", position_bias, 0.0f, 1.0f); - CheckParameterLowerUpperBound("position-bias-pval", position_bias_pval, 0.0f, 1.0f); - // CheckParameterLowerUpperBound("tune-xbias", xbias_tune, 0.001f, 1000.0f); - CheckParameterLowerUpperBound("tune-sbias", sbias_tune, 0.001f, 1000.0f); - CheckParameterLowerBound ("lod-multiplier", lod_multiplier, 0.6f); - - CheckParameterLowerBound ("snp-min-cov-each-strand", filter_snps.min_cov_each_strand, 0); - CheckParameterLowerBound ("snp-min-variant-score", filter_snps.min_quality_score, 0.0f); - CheckParameterLowerUpperBound("snp-min-allele-freq", filter_snps.min_allele_freq, 0.0f, 1.0f); - CheckParameterLowerBound ("snp-min-coverage", filter_snps.min_cov, 0); - CheckParameterLowerUpperBound("snp-strand-bias", filter_snps.strand_bias_threshold, 0.5f, 1.0f); - CheckParameterLowerUpperBound("snp-strand-bias-pval", filter_snps.strand_bias_pval_threshold, 0.0f, 1.0f); - CheckParameterLowerBound ("snp-min-var-coverage", filter_snps.min_var_cov, 0); -// CheckParameterLowerBound ("snp-beta-bias", filter_snps.beta_bias_filter, 0.0f); - - CheckParameterLowerBound ("mnp-min-cov-each-strand", filter_mnp.min_cov_each_strand, 0); - CheckParameterLowerBound ("mnp-min-variant-score", filter_mnp.min_quality_score, 0.0f); - CheckParameterLowerUpperBound("mnp-min-allele-freq", filter_mnp.min_allele_freq, 0.0f, 1.0f); - CheckParameterLowerBound ("mnp-min-coverage", filter_mnp.min_cov, 0); - CheckParameterLowerUpperBound("mnp-strand-bias", filter_mnp.strand_bias_threshold, 0.5f, 1.0f); - CheckParameterLowerUpperBound("mnp-strand-bias-pval", filter_mnp.strand_bias_pval_threshold, 0.0f, 1.0f); - CheckParameterLowerBound ("mnp-min-var-coverage", filter_mnp.min_var_cov, 0); - - CheckParameterLowerBound ("indel-min-cov-each-strand", filter_hp_indel.min_cov_each_strand, 0); - CheckParameterLowerBound ("indel-min-variant-score", filter_hp_indel.min_quality_score, 0.0f); - CheckParameterLowerUpperBound("indel-min-allele-freq", filter_hp_indel.min_allele_freq, 0.0f, 1.0f); - CheckParameterLowerBound ("indel-min-coverage", filter_hp_indel.min_cov, 0); - CheckParameterLowerUpperBound("indel-strand-bias", filter_hp_indel.strand_bias_threshold, 0.5f, 1.0f); - CheckParameterLowerUpperBound("indel-strand-bias-pval", filter_hp_indel.strand_bias_pval_threshold, 0.0f, 1.0f); -// CheckParameterLowerBound ("indel-beta-bias", filter_hp_indel.beta_bias_filter, 0.0f); - CheckParameterLowerBound ("indel-min-var-coverage", filter_hp_indel.min_var_cov, 0); - - CheckParameterLowerBound ("hotspot-min-cov-each-strand", filter_hotspot.min_cov_each_strand, 0); - CheckParameterLowerBound ("hotspot-min-variant-score", filter_hotspot.min_quality_score, 0.0f); - CheckParameterLowerUpperBound("hotspot-min-allele-freq", filter_hotspot.min_allele_freq, 0.0f, 1.0f); - CheckParameterLowerBound ("hotspot-min-coverage", filter_hotspot.min_cov, 0); - CheckParameterLowerUpperBound("hotspot-strand-bias", filter_hotspot.strand_bias_threshold, 0.5f, 1.0f); - CheckParameterLowerUpperBound("hotspot-strand-bias-pval", filter_hotspot.strand_bias_pval_threshold, 0.0f, 1.0f); -// CheckParameterLowerBound ("hotspot-beta-bias", filter_hotspot.beta_bias_filter, 0.0f); - CheckParameterLowerBound ("hotspot-min-var-coverage", filter_hotspot.min_var_cov, 0); + CheckParameterLowerBound ("data-quality-stringency", data_quality_stringency, 0.0f); + CheckParameterLowerUpperBound("read-rejection-threshold", read_rejection_threshold, 0.0f, 1.0f); + CheckParameterLowerUpperBound ("downsample-to-coverage", downSampleCoverage, 20, 200000); + CheckParameterLowerUpperBound("position-bias-ref-fraction",position_bias_ref_fraction, 0.0f, 1.0f); + CheckParameterLowerUpperBound("position-bias", position_bias, 0.0f, 1.0f); + CheckParameterLowerUpperBound("position-bias-pval", position_bias_pval, 0.0f, 1.0f); + CheckParameterLowerUpperBound("tune-sbias", sbias_tune, 0.001f, 1000.0f); + CheckParameterLowerBound ("lod-multiplier", lod_multiplier, 0.0f); + CheckParameterLowerBound ("tag-sim-max-cov", tag_sim_max_cov, 0); + CheckParameterLowerUpperBound("min-read-ratio-for-fd", min_ratio_for_fd, 0.0f, 1.0f); + CheckParameterLowerBound ("fd-nonsnp-min-var-cov", fd_nonsnp_min_var_cov, 0); + + filter_variant.CheckParameterLimits(); + filter_snp.CheckBasicFiltersLimits(); + filter_mnp.CheckBasicFiltersLimits(); + filter_hp_indel.CheckBasicFiltersLimits(); + filter_hotspot.CheckBasicFiltersLimits(); + filter_fd_0.CheckBasicFiltersLimits(); + filter_fd_5.CheckBasicFiltersLimits(); + filter_fd_10.CheckBasicFiltersLimits(); } // ------------------------------------------------------ @@ -671,7 +638,13 @@ void ControlCallAndFilters::SetOpts(OptArgs &opts, Json::Value& tvc_params) { use_lod_filter = RetrieveParameterBool(opts, tvc_params, '-', "use-lod-filter", false); lod_multiplier = RetrieveParameterDouble(opts, tvc_params, '-', "lod-multiplier",0.6f); + tag_sim_max_cov = RetrieveParameterInt (opts, tvc_params, '-', "tag-sim-max-cov", 20); downSampleCoverage = RetrieveParameterInt (opts, tvc_params, '-', "downsample-to-coverage", 2000); + + // VCF record filters XXX + //filter_by_target = RetrieveParameterBool (opts, tvc_params, '-', "filter-by-target", true); + //hotspot_positions_only = RetrieveParameterBool (opts, tvc_params, '-', "hotspot-positions-only", false); + //hotspot_variants_only = RetrieveParameterBool (opts, tvc_params, '-', "hotspot-variants-only", false); //xbias_tune = RetrieveParameterDouble(opts, tvc_params, '-', "tune-xbias", 0.005f); sbias_tune = RetrieveParameterDouble(opts, tvc_params, '-', "tune-sbias", 0.01f); @@ -680,43 +653,34 @@ void ControlCallAndFilters::SetOpts(OptArgs &opts, Json::Value& tvc_params) { suppress_nocall_genotypes = RetrieveParameterBool (opts, tvc_params, '-', "suppress-nocall-genotypes", true); suppress_no_calls = RetrieveParameterBool (opts, tvc_params, '-', "suppress-no-calls", true); - heal_snps = RetrieveParameterBool (opts, tvc_params, '-', "heal-snps", true); - - // SNPS are my usual variants - filter_snps.min_cov_each_strand = RetrieveParameterInt (opts, tvc_params, 'C', "snp-min-cov-each-strand", 0); - filter_snps.min_quality_score = RetrieveParameterDouble(opts, tvc_params, 'B', "snp-min-variant-score", 10.0); - filter_snps.min_allele_freq = RetrieveParameterDouble(opts, tvc_params, 'A', "snp-min-allele-freq", 0.2); - filter_snps.min_cov = RetrieveParameterInt (opts, tvc_params, 'k', "snp-min-coverage", 6); - filter_snps.strand_bias_threshold = RetrieveParameterDouble(opts, tvc_params, 's', "snp-strand-bias", 0.95); - filter_snps.strand_bias_pval_threshold= RetrieveParameterDouble(opts, tvc_params, 's', "snp-strand-bias-pval", 1.0); - filter_snps.min_var_cov = RetrieveParameterInt (opts, tvc_params, '-', "snp-min-var-coverage", 0); - - filter_mnp.min_cov_each_strand = RetrieveParameterInt (opts, tvc_params, '-', "mnp-min-cov-each-strand", filter_snps.min_cov_each_strand); - filter_mnp.min_quality_score = RetrieveParameterDouble(opts, tvc_params, '-', "mnp-min-variant-score", filter_snps.min_quality_score); - filter_mnp.min_allele_freq = RetrieveParameterDouble(opts, tvc_params, 'H', "mnp-min-allele-freq", filter_snps.min_allele_freq); - filter_mnp.min_cov = RetrieveParameterInt (opts, tvc_params, '-', "mnp-min-coverage", filter_snps.min_cov); - filter_mnp.strand_bias_threshold = RetrieveParameterDouble(opts, tvc_params, '-', "mnp-strand-bias", filter_snps.strand_bias_threshold); - filter_mnp.strand_bias_pval_threshold= RetrieveParameterDouble(opts, tvc_params, 's', "mnp-strand-bias-pval", filter_snps.strand_bias_pval_threshold); - filter_mnp.min_var_cov = RetrieveParameterInt (opts, tvc_params, '-', "mnp-min-var-coverage", filter_snps.min_var_cov); - - // hp_indels are more complex - filter_hp_indel.min_cov_each_strand = RetrieveParameterInt (opts, tvc_params, '-', "indel-min-cov-each-strand", 1); - filter_hp_indel.min_quality_score = RetrieveParameterDouble(opts, tvc_params, '-', "indel-min-variant-score", 10.0); - filter_hp_indel.min_allele_freq = RetrieveParameterDouble(opts, tvc_params, '-', "indel-min-allele-freq", 0.2); - filter_hp_indel.min_cov = RetrieveParameterInt (opts, tvc_params, '-', "indel-min-coverage", 15); - filter_hp_indel.strand_bias_threshold = RetrieveParameterDouble(opts, tvc_params, 'S', "indel-strand-bias", 0.85); - filter_hp_indel.strand_bias_pval_threshold= RetrieveParameterDouble(opts, tvc_params, 's', "indel-strand-bias-pval", 1.0); - filter_hp_indel.min_var_cov = RetrieveParameterInt (opts, tvc_params, '-', "indel-min-var-coverage", filter_snps.min_var_cov); - // derive hotspots by default from SNPs - // override from command line or json - filter_hotspot.min_cov_each_strand = RetrieveParameterInt (opts, tvc_params, '-', "hotspot-min-cov-each-strand", filter_snps.min_cov_each_strand); - filter_hotspot.min_quality_score = RetrieveParameterDouble(opts, tvc_params, '-', "hotspot-min-variant-score", filter_snps.min_quality_score); - filter_hotspot.min_allele_freq = RetrieveParameterDouble(opts, tvc_params, 'H', "hotspot-min-allele-freq", filter_snps.min_allele_freq); - filter_hotspot.min_cov = RetrieveParameterInt (opts, tvc_params, '-', "hotspot-min-coverage", filter_snps.min_cov); - filter_hotspot.strand_bias_threshold = RetrieveParameterDouble(opts, tvc_params, '-', "hotspot-strand-bias", filter_snps.strand_bias_threshold); - filter_hotspot.strand_bias_pval_threshold= RetrieveParameterDouble(opts, tvc_params, 's', "hotspot-strand-bias-pval", filter_snps.strand_bias_pval_threshold); - filter_hotspot.min_var_cov = RetrieveParameterInt (opts, tvc_params, '-', "hotspot-min-var-coverage", filter_snps.min_var_cov); - + // Deprecate heal-snps + cleanup_unlikely_candidates = RetrieveParameterBool (opts, tvc_params, '-', "heal-snps", true); + cleanup_unlikely_candidates = RetrieveParameterBool (opts, tvc_params, '-', "cleanup-unlikely-candidates", cleanup_unlikely_candidates); + report_ppa = RetrieveParameterBool (opts, tvc_params, '-', "report-ppa", false); + hotspots_as_de_novo = RetrieveParameterBool (opts, tvc_params, '-', "hotspots-as-de-novo", false); + disable_filters = RetrieveParameterBool (opts, tvc_params, '-', "disable-filters", false); + + // Flow-disruption related + fd_nonsnp_min_var_cov = RetrieveParameterInt (opts, tvc_params, '-', "fd-nonsnp-min-var-cov", 0); + use_fd_param = RetrieveParameterBool (opts, tvc_params, '-', "use-fd-param", false); + min_ratio_for_fd = RetrieveParameterDouble(opts, tvc_params, '-', "min-ratio-for-fd", 0.1); + + filter_snp.SetBasicFilterOpts(opts, tvc_params, NULL); + // Default: mnp behaves like snp + filter_mnp.SetBasicFilterOpts(opts, tvc_params, &filter_snp); + filter_hp_indel.SetBasicFilterOpts(opts, tvc_params, NULL); + + filter_fd_0.SetBasicFilterOpts(opts, tvc_params, NULL); + // Default: fd-5 behaves like fd-0. + filter_fd_5.SetBasicFilterOpts(opts, tvc_params, &filter_fd_0); + filter_fd_10.SetBasicFilterOpts(opts, tvc_params, NULL); + // Default: HS behaves like the most aggresive one. + if (use_fd_param){ + filter_hotspot.SetBasicFilterOpts(opts, tvc_params, &filter_fd_10); + } + else{ + filter_hotspot.SetBasicFilterOpts(opts, tvc_params, &filter_snp); + } } // ============================================================================= @@ -725,21 +689,10 @@ void ProgramControlSettings::CheckParameterLimits() { CheckParameterLowerUpperBound ("num-threads", nThreads, 1, 128); CheckParameterLowerUpperBound ("num-variants-per-thread", nVariantsPerThread, 1, 10000); - for(unsigned int i_freq = 0; i_freq < snp_multi_min_allele_freq.size(); ++i_freq){ + + for(unsigned int i_freq = 0; i_freq < multi_min_allele_freq.size(); ++i_freq){ string identifier = "multi-min-allele-freq[" + convertToString(i_freq) + "]"; - CheckParameterLowerUpperBound (identifier, snp_multi_min_allele_freq[i_freq], 0.0f, 1.0f); - } - for(unsigned int i_freq = 0; i_freq < mnp_multi_min_allele_freq.size(); ++i_freq){ - string identifier = "mnp-multi-min-allele-freq[" + convertToString(i_freq) + "]"; - CheckParameterLowerUpperBound (identifier, mnp_multi_min_allele_freq[i_freq], 0.0f, 1.0f); - } - for(unsigned int i_freq = 0; i_freq < indel_multi_min_allele_freq.size(); ++i_freq){ - string identifier = "indel-multi-min-allele-freq[" + convertToString(i_freq) + "]"; - CheckParameterLowerUpperBound (identifier, indel_multi_min_allele_freq[i_freq], 0.0f, 1.0f); - } - for(unsigned int i_freq = 0; i_freq < hotspot_multi_min_allele_freq.size(); ++i_freq){ - string identifier = "hotspot-multi-min-allele-freq[" + convertToString(i_freq) + "]"; - CheckParameterLowerUpperBound (identifier, hotspot_multi_min_allele_freq[i_freq], 0.0f, 1.0f); + CheckParameterLowerUpperBound (identifier, multi_min_allele_freq[i_freq], 0.0f, 1.0f); } } @@ -764,19 +717,10 @@ void ProgramControlSettings::SetOpts(OptArgs &opts, Json::Value &tvc_params) { use_SSE_basecaller = RetrieveParameterBool (opts, tvc_params, '-', "use-sse-basecaller", true); do_indel_assembly = RetrieveParameterBool (opts, tvc_params, '-', "do-indel-assembly", true); + is_multi_min_allele_freq = RetrieveParameterBool (opts, tvc_params, '-', "output-multi-min-allele-freq", false); - RetrieveParameterVectorFloat(opts, tvc_params, '-', "snp-multi-min-allele-freq", "0.05,0.1,0.15,0.2", snp_multi_min_allele_freq); - string snp_multi_min_allele_freq_str = ""; - for(unsigned i_freq = 0; i_freq < snp_multi_min_allele_freq.size(); ++i_freq){ - snp_multi_min_allele_freq_str += convertToString(snp_multi_min_allele_freq[i_freq]); - if(i_freq < snp_multi_min_allele_freq.size() - 1){ - snp_multi_min_allele_freq_str += ","; - } - } - RetrieveParameterVectorFloat(opts, tvc_params, '-', "mnp-multi-min-allele-freq", snp_multi_min_allele_freq_str, mnp_multi_min_allele_freq); - RetrieveParameterVectorFloat(opts, tvc_params, '-', "indel-multi-min-allele-freq", "0.05,0.1,0.15,0.2", indel_multi_min_allele_freq); - RetrieveParameterVectorFloat(opts, tvc_params, '-', "hotspot-multi-min-allele-freq", "0.05,0.1,0.15,0.2", hotspot_multi_min_allele_freq); + RetrieveParameterVectorFloat(opts, tvc_params, '-', "multi-min-allele-freq", "0.05,0.1,0.15,0.2", multi_min_allele_freq); } // =========================================================================== @@ -793,28 +737,6 @@ bool ExtendParameters::ValidateAndCanonicalizePath(string &path) return true; } -int mkpath(std::string s,mode_t mode) -{ - size_t pre=0,pos; - std::string dir; - int mdret = 0; - - if(s[s.size()-1]!='/'){ - // force trailing / so we can handle everything in loop - s+='/'; - } - - while((pos=s.find_first_of('/',pre))!=std::string::npos){ - dir=s.substr(0,pos++); - pre=pos; - if(dir.size()==0) continue; // if leading / first time is 0 length - if((mdret=mkdir(dir.c_str(),mode)) && errno!=EEXIST){ - return mdret; - } - } - return mdret; -} - void ExtendParameters::SetupFileIO(OptArgs &opts, Json::Value& tvc_params) { // freeBayes slot fasta = opts.GetFirstString('r', "reference", ""); @@ -825,9 +747,9 @@ void ExtendParameters::SetupFileIO(OptArgs &opts, Json::Value& tvc_params) { ValidateAndCanonicalizePath(fasta); // freeBayes slot - blacklistFile = opts.GetFirstString('l', "blacklist-vcf", ""); + blacklistFile = opts.GetFirstString('l', "sse-vcf", ""); if (blacklistFile.empty()) { - cerr << "INFO: No blacklist VCF file specified via -l,--blacklist-vcf" << endl; + cerr << "INFO: No sse VCF file specified via -l,--sse-vcf" << endl; } else ValidateAndCanonicalizePath(blacklistFile); @@ -838,7 +760,13 @@ void ExtendParameters::SetupFileIO(OptArgs &opts, Json::Value& tvc_params) { else ValidateAndCanonicalizePath(variantPriorsFile); - sseMotifsFileName = opts.GetFirstString('e', "error-motifs", ""); + sseMotifsDir = opts.GetFirstString('-', "error-motifs-dir", ""); + sseMotifsFileName = RetrieveParameterString (opts, tvc_params, 'e', "error-motifs", ""); + size_t pos = sseMotifsFileName.rfind("/"); + if ((!sseMotifsDir.empty()) && (pos != std::string::npos) && (pos < sseMotifsFileName.length() - 1)) { + sseMotifsFileName = sseMotifsFileName.substr(pos + 1); + } + if (sseMotifsDir != "") {sseMotifsFileName = sseMotifsDir + "/" + sseMotifsFileName;} sseMotifsProvided = true; if (sseMotifsFileName.empty()) { sseMotifsProvided = false; @@ -861,17 +789,45 @@ void ExtendParameters::SetupFileIO(OptArgs &opts, Json::Value& tvc_params) { mkpath(outputDir, S_IRWXU | S_IRWXG | S_IROTH | S_IXOTH); ValidateAndCanonicalizePath(outputDir); - outputFile = opts.GetFirstString('o', "output-vcf", ""); - if (outputFile.empty()) { - cerr << "Fatal ERROR: Output VCF filename not specified via -o" << endl; + // Parse output file names + small_variants_vcf = opts.GetFirstString('o', "output-vcf", "small_variants.vcf"); + if (small_variants_vcf.empty()) { + cerr << "Fatal ERROR: Output VCF filename not specified via -o or --output-vcf" << endl; + exit(1); + } + + indel_assembly_vcf = opts.GetFirstString('-', "assembly-vcf", "indel_assembly.vcf"); + if (indel_assembly_vcf.empty()) { + cerr << "Fatal ERROR: Assembly VCF filename not specified via --assembly-vcf" << endl; exit(1); } - // Are those file names? + // XXX VCF merging not being done in TVC 5.4 but in the varriant caller pipeline + //merged_vcf = opts.GetFirstString('o', "merged-vcf", "TSVC_variants.vcf"); + //if (merged_vcf.empty()) { + // cerr << "Fatal ERROR: Merged VCF filename not specified via --merged-vcf" << endl; + // exit(1); + //} + // Gvcf file name is the same as merged vcf file, but with different file ending + //string vcf_file_end(".vcf"); + //merged_genome_vcf = merged_vcf.substr(0, merged_vcf.find(".vcf")) + ".genome.vcf"; + + string tmp = opts.GetFirstString('-', "candidate-list-file", ""); + if (tmp.size() > 0) candidate_list = outputDir+ "/" + tmp; + /* + tmp = opts.GetFirstString('-', "black-listed-file", ""); + if (tmp.size() > 0) black_listed = outputDir + "/" + tmp; + */ + black_listed = outputDir + "/black_listed.vcf"; + + // Are those file names? - Nope, only the first one! postprocessed_bam = opts.GetFirstString('-', "postprocessed-bam", ""); sampleName = opts.GetFirstString('g', "sample-name", ""); force_sample_name = opts.GetFirstString('-', "force-sample-name", ""); - + // TS 5.4: Multisample currently produces core dumps. Deactivated unless override-limits is specified. + //multisample = RetrieveParameterBool (opts, tvc_params, '-', "multisample-analysis", false); + // Allow command line only and do not report in log. + multisample = opts.GetFirstBoolean('-', "multisample-analysis", false); } // ------------------------------------------------------------ @@ -879,9 +835,11 @@ void ExtendParameters::SetupFileIO(OptArgs &opts, Json::Value& tvc_params) { void ExtendParameters::SetFreeBayesParameters(OptArgs &opts, Json::Value& fb_params) { // FreeBayes parameters // primarily used in candidate generation - + // Note: Abuse of fb_params here. The following parameters will get into TargetsManager and won't be the part of Freebayes. targets = opts.GetFirstString('t', "target-file", ""); trim_ampliseq_primers = opts.GetFirstBoolean('-', "trim-ampliseq-primers", false); + min_cov_fraction = RetrieveParameterDouble(opts, fb_params, '-', "min-cov-fraction", 0.0); + if (targets.empty() and trim_ampliseq_primers) { cerr << "ERROR: --trim-ampliseq-primers enabled but no --target-file provided" << endl; exit(1); @@ -891,7 +849,8 @@ void ExtendParameters::SetFreeBayesParameters(OptArgs &opts, Json::Value& fb_par allowSNPs = RetrieveParameterBool (opts, fb_params, '-', "allow-snps", true); allowMNPs = RetrieveParameterBool (opts, fb_params, '-', "allow-mnps", true); allowComplex = RetrieveParameterBool (opts, fb_params, '-', "allow-complex", false); - mergeLookAhead = RetrieveParameterInt (opts, fb_params, '-', "merge-variant-lookahead", 3); + mergeLookAhead = RetrieveParameterInt (opts, fb_params, '-', "merge-variant-lookahead", -1); + output_allele_cigar = RetrieveParameterBool (opts, fb_params, '-', "allele-cigar-output", false); // deprecated: // leftAlignIndels = RetrieveParameterBool (opts, fb_params, '-', "left-align-indels", false); @@ -899,25 +858,24 @@ void ExtendParameters::SetFreeBayesParameters(OptArgs &opts, Json::Value& fb_par //useBestNAlleles = 0; useBestNAlleles = RetrieveParameterInt (opts, fb_params, 'm', "use-best-n-alleles", 2); + useBestNTotalAlleles = RetrieveParameterInt (opts, fb_params, '-', "use-best-n-nonsnp-alleles", 0); + max_alt_num = RetrieveParameterInt (opts, fb_params, '-', "max-alt-num", 20); onlyUseInputAlleles = RetrieveParameterBool (opts, fb_params, '-', "use-input-allele-only", false); min_mapping_qv = RetrieveParameterInt (opts, fb_params, 'M', "min-mapping-qv", 4); read_snp_limit = RetrieveParameterInt (opts, fb_params, 'U', "read-snp-limit", 10); readMaxMismatchFraction = RetrieveParameterDouble(opts, fb_params, 'z', "read-max-mismatch-fraction", 1.0); + read_mismatch_limit = RetrieveParameterInt (opts, fb_params, '-', "read-mismatch-limit", 0); maxComplexGap = RetrieveParameterInt (opts, fb_params, '!', "max-complex-gap", 1); // read from json or command line, otherwise default to snp frequency - minAltFraction = RetrieveParameterDouble(opts, fb_params, '-', "gen-min-alt-allele-freq", my_controls.filter_snps.min_allele_freq); - minCoverage = RetrieveParameterInt (opts, fb_params, '-', "gen-min-coverage", my_controls.filter_snps.min_cov); + minAltFraction = RetrieveParameterDouble(opts, fb_params, '-', "gen-min-alt-allele-freq", my_controls.filter_snp.min_allele_freq); + minCoverage = RetrieveParameterInt (opts, fb_params, '-', "gen-min-coverage", my_controls.filter_snp.min_cov); minIndelAltFraction = RetrieveParameterDouble(opts, fb_params, '-', "gen-min-indel-alt-allele-freq", my_controls.filter_hp_indel.min_allele_freq); //set up debug levels - if (program_flow.DEBUG > 0) - debug = true; - - if (program_flow.inputPositionsOnly) { - processInputPositionsOnly = true; - } + debug = program_flow.DEBUG > 0; + processInputPositionsOnly = program_flow.inputPositionsOnly; - if (variantPriorsFile.empty() && (processInputPositionsOnly || onlyUseInputAlleles) ) { + if (variantPriorsFile.empty() and (processInputPositionsOnly or onlyUseInputAlleles) ) { cerr << "ERROR: Parameter error - Process-input-positions-only: " << processInputPositionsOnly << " use-input-allele-only: " << onlyUseInputAlleles << " : Specified without Input VCF File " << endl; exit(1); } @@ -957,29 +915,65 @@ void ExtendParameters::CheckParameterLimits() { program_flow.CheckParameterLimits(); // Checking FreeBayes parameters + CheckParameterLowerUpperBound("min-cov-fraction", min_cov_fraction, 0.0f, 1.0f); CheckParameterLowerUpperBound ("use-best-n-alleles", useBestNAlleles, 0, 20); + CheckParameterLowerUpperBound ("use-best-n-nonsnp-alleles", useBestNTotalAlleles, 0, 20); CheckParameterLowerBound ("min-mapping-qv", min_mapping_qv, 0); CheckParameterLowerBound ("read-snp-limit", read_snp_limit, 0); CheckParameterLowerUpperBound("read-max-mismatch-fraction", readMaxMismatchFraction, 0.0f, 1.0f); - + CheckParameterLowerBound ("read-mismatch-limit", read_mismatch_limit, 0); CheckParameterLowerUpperBound("gen-min-alt-allele-freq", minAltFraction, 0.0, 1.0); CheckParameterLowerBound ("gen-min-coverage", minCoverage, 0); + CheckParameterLowerBound ("max-alt-num", max_alt_num, 1); CheckParameterLowerUpperBound("gen-min-indel-alt-allele-freq", minIndelAltFraction, 0.0, 1.0); + // Checking TagTrimmerParameters + CheckParameterLowerUpperBound ("tag-trim-method", tag_trimmer_parameters.tag_trim_method, 0, 1); + CheckParameterLowerBound ("min-tag-fam-size", tag_trimmer_parameters.min_family_size, 1); + CheckParameterLowerBound ("indel-func-size-offset", tag_trimmer_parameters.indel_func_size_offset, 0); } +void ExtendParameters::SetMolecularTagTrimmerOpt(Json::Value& tvc_params) +{ + enum TagFilterOptions { + kneed_only_prefix_tag, + kneed_only_suffix_tag, + kneed_all_tags, + }; + + enum TagTrimMethod { + kStrictTrim, + kSloppyTrim, + kUnknownTrim + }; + + tag_trimmer_parameters.suppress_mol_tags = RetrieveParameterBool (opts, tvc_params, '-', "suppress-mol-tags", false); + tag_trimmer_parameters.min_family_size = RetrieveParameterInt (opts, tvc_params, '-', "min-tag-fam-size", 3); + tag_trimmer_parameters.indel_func_size_offset = RetrieveParameterInt (opts, tvc_params, '-', "indel-func-size-offset", 0); + string trim_method = RetrieveParameterString (opts, tvc_params, '-', "tag-trim-method", "sloppy-trim"); + + if (trim_method == "sloppy-trim" or trim_method == "sloppy_trim") + tag_trimmer_parameters.tag_trim_method = kSloppyTrim; + else if (trim_method == "strict-trim" or trim_method == "strict_trim") + tag_trimmer_parameters.tag_trim_method = kStrictTrim; + else { + tag_trimmer_parameters.tag_trim_method = kUnknownTrim; + } + // tvc requires need all tags + tag_trimmer_parameters.tag_filter_method = kneed_all_tags; + +} // ------------------------------------------------------------ ExtendParameters::ExtendParameters(int argc, char** argv) { // i/o parameters: - fasta = ""; // -f --fasta-reference - targets = ""; // -t --targets - outputFile = ""; + // file names use default string constructor = "" // operation parameters trim_ampliseq_primers = false; + min_cov_fraction = 0.0f; useDuplicateReads = false; // -E --use-duplicate-reads useBestNAlleles = 0; // -n --use-best-n-alleles allowIndels = true; // -i --no-indels @@ -990,6 +984,7 @@ ExtendParameters::ExtendParameters(int argc, char** argv) onlyUseInputAlleles = false; min_mapping_qv = 0; // -m --min-mapping-quality readMaxMismatchFraction = 1.0; // -z --read-max-mismatch-fraction + read_mismatch_limit = 0; read_snp_limit = 10000000; // -$ --read-snp-limit minAltFraction = 0.2; // require 20% of reads from sample to be supporting the same alternate to consider minIndelAltFraction = 0.2; @@ -1016,12 +1011,6 @@ ExtendParameters::ExtendParameters(int argc, char** argv) exit(0); } - // enable floating point exceptions during program execution - if (opts.GetFirstBoolean('-', "float-exceptions", true)) { - cout << "TVC: Floating point exceptions enabled." << endl; - feraiseexcept(FE_DIVBYZERO | FE_INVALID | FE_OVERFLOW); - } //*/ - Json::Value tvc_params(Json::objectValue); Json::Value freebayes_params(Json::objectValue); Json::Value params_meta(Json::objectValue); @@ -1032,22 +1021,28 @@ ExtendParameters::ExtendParameters(int argc, char** argv) my_controls.SetOpts(opts, tvc_params); my_eval_control.SetOpts(opts, tvc_params); program_flow.SetOpts(opts, tvc_params); - tag_trimmer_parameters = MolecularTagTrimmer::ReadOpts(opts); - // Preserve the data for all flows if we want to do rich diagnostic - // Otherwise we only keep the data for test flows - my_eval_control.preserve_full_data = program_flow.rich_json_diagnostic; + // Create output directory for diagnostic if desired + if (program_flow.rich_json_diagnostic || program_flow.minimal_diagnostic) { + json_plot_dir = outputDir + "/json_diagnostic/"; + mkpath(json_plot_dir, S_IRWXU | S_IRWXG | S_IROTH | S_IXOTH); + ValidateAndCanonicalizePath(outputDir); + } + + // tag_trimmer_parameters = MolecularTagTrimmer::ReadOpts(opts); // not read from json! + // Get tag trimmer parameters + SetMolecularTagTrimmerOpt(tvc_params); // Dummy lines for HP recalibration recal_model_file_name = opts.GetFirstString ('-', "model-file", ""); recalModelHPThres = opts.GetFirstInt('-', "recal-model-hp-thres", 4); - prefixExclusion = opts.GetFirstInt('-', "prefix-exclude", 6); - cerr << "prefix-exclude = " << prefixExclusion << endl; - SetFreeBayesParameters(opts, freebayes_params); bool overrideLimits = RetrieveParameterBool (opts, tvc_params, '-', "override-limits", false); + prefixExclusion = opts.GetFirstInt('-', "prefix-exclude", 6); + cerr << "prefix-exclude = " << prefixExclusion << endl; + params_meta_name = params_meta.get("name",string()).asString(); params_meta_details = params_meta.get("configuration_name", string()).asString(); string external_file = params_meta.get("external_file", string()).asString(); @@ -1072,39 +1067,20 @@ ExtendParameters::ExtendParameters(int argc, char** argv) // opts.CheckNoLeftovers(); // Sanity checks on input variables once all are set. - if (!overrideLimits) - CheckParameterLimits(); - -} - -// check whether parameter contains only the characters in context -bool CheckParameterStringContext(string identifier, string ¶meter, const string &contains_only, const string &default_value){ - bool is_ok = true; // for the case where parameter is an empty string. Will return true if parameter is empty. + if (!overrideLimits){ + if (multisample){ + cout << "Limit check parameter multisample-analysis: Using multsample_analysis=false instead." << endl; + multisample = false; + } - cout << "Check parameter " << identifier << ": " - << parameter << " contains only " << contains_only << "? "; + CheckParameterLimits(); + } - for(unsigned int i = 0; i < parameter.size(); ++i){ - is_ok = false; - for(unsigned int j = 0; j < contains_only.size(); ++j){ - if(parameter[i] == contains_only[j]){ - is_ok = true; - break; - } - } - if(not is_ok){ - break; - } - } - if(is_ok){ - cout<< "OK!"; - }else{ - cout<< "The parameter "<< identifier - << " should only contain the characters in " << contains_only <<". " - << "Using the default value " << default_value <<" instead!"; - } - cout << endl; - return is_ok; + if (my_controls.disable_filters){ + cout << "tvc will disable all the post-evaluation filters." << endl; + my_controls.suppress_reference_genotypes = false; + my_controls.suppress_nocall_genotypes = false; + my_controls.cleanup_unlikely_candidates = false; + my_controls.suppress_no_calls = false; + } } - - diff --git a/Analysis/VariantCaller/Bookkeeping/ExtendParameters.h b/Analysis/VariantCaller/Bookkeeping/ExtendParameters.h index d165c0ff..b9fdffd0 100644 --- a/Analysis/VariantCaller/Bookkeeping/ExtendParameters.h +++ b/Analysis/VariantCaller/Bookkeeping/ExtendParameters.h @@ -10,11 +10,18 @@ #include #include #include "OptArgs.h" +#include "OptBase.h" #include "json/json.h" #include "MolecularTagTrimmer.h" +#include +#include +#include using namespace std; +int mkpath(std::string s,mode_t mode); + +//bool RetrieveParameterBool(OptArgs &opts, Json::Value& json, char short_name, const string& long_name_hyphens, bool default_value); // provide an interface to set useful parameters across the objects class EnsembleEvalTuningParameters { @@ -22,6 +29,7 @@ class EnsembleEvalTuningParameters { float germline_prior_strength; // how concentrated are we at 0,0.5,1.0 frequency for germline calls float outlier_prob; // 1-data_reliability int heavy_tailed; // how heavy are the tails of my distribution to resist mis-shapen items = CrossHypotheses + bool adjust_sigma; // If true, sigma^2 = (dof-2) / dof * E[r^2] (where dof = 2*heavy_tail - 1), else sigma^2 = E[r^2] float prediction_precision; // damper_bias = bias_generator - how likely the predictions are to be accurately calibrated float min_delta_for_flow; // select flows to test based on this minimum level @@ -45,8 +53,6 @@ class EnsembleEvalTuningParameters { int min_detail_level_for_fast_scan; bool try_few_restart_freq; - bool preserve_full_data; // Preserve the data for all flows if true, otherwise preserve the data only at test flows - EnsembleEvalTuningParameters() { germline_prior_strength = 0.0f; outlier_prob = 0.01f; @@ -67,17 +73,12 @@ class EnsembleEvalTuningParameters { filter_deletion_bias = 10.0f; filter_insertion_bias = 10.0f; max_detail_level = 0; - min_detail_level_for_fast_scan = 2500; + min_detail_level_for_fast_scan = 0; try_few_restart_freq = false; - preserve_full_data = false; //use_all_compare_for_test_flows = false; }; - float DataReliability() { - return(1.0f - outlier_prob); - }; - void CheckParameterLimits(); void SetOpts(OptArgs &opts, Json::Value& tvc_params); @@ -85,27 +86,20 @@ class EnsembleEvalTuningParameters { class BasicFilters { public: - float min_allele_freq; - - float strand_bias_threshold; - float strand_bias_pval_threshold; - - float min_quality_score; - - int min_cov; - int min_cov_each_strand; - int min_var_cov; - - BasicFilters() { - min_allele_freq = 0.2f; - strand_bias_threshold = 0.8f; - strand_bias_pval_threshold = 1.0f; - - min_cov = 3; - min_cov_each_strand = 3; - min_quality_score = 2.5f; - min_var_cov = 0; - }; + float min_allele_freq = -1.0f; + float strand_bias_threshold = -1.0f; + float strand_bias_pval_threshold = -1.0f; + float min_quality_score = -1.0f; + int min_cov = -1; + int min_cov_each_strand = -1; + int min_var_cov = -1; + string my_type; + string underscored_my_type; + + BasicFilters() {}; + BasicFilters(const string& default_type); + void CheckBasicFiltersLimits(); + void SetBasicFilterOpts(OptArgs &opts, Json::Value& tvc_params, BasicFilters* default_param); }; @@ -130,6 +124,11 @@ class ClassifyFilters { // treat non hp indels as hp indels bool indel_as_hpindel; + // the hp-indel filter + vector filter_hp_indel_hrun; + vector filter_hp_ins_len; + vector filter_hp_del_len; + ClassifyFilters() { hp_max_length = 11; @@ -142,6 +141,10 @@ class ClassifyFilters { realignment_threshold = 1.0; indel_as_hpindel = false; + + filter_hp_indel_hrun = {7, 8}; + filter_hp_ins_len = {0, 0}; + filter_hp_del_len = {0, 0}; }; void SetOpts(OptArgs &opts, Json::Value & tvc_params); void CheckParameterLimits(); @@ -163,29 +166,49 @@ class ControlCallAndFilters { bool suppress_reference_genotypes; bool suppress_nocall_genotypes; - bool heal_snps; // if a snp is the best allele, discard all others + bool cleanup_unlikely_candidates; // if a snp is the best allele, discard all others bool suppress_no_calls; + bool report_ppa; + bool hotspots_as_de_novo; + bool disable_filters; // position bias probably should not be variant specific - bool use_position_bias; + bool use_position_bias; float position_bias_ref_fraction; float position_bias; float position_bias_pval; - // Dima's LOD filter - bool use_lod_filter; + // LOD filter + bool use_lod_filter; float lod_multiplier; + // filter's for mol tags + int tag_sim_max_cov; + + // flow-disruptivenss stuff + bool use_fd_param ; + float min_ratio_for_fd; + int fd_nonsnp_min_var_cov; + ClassifyFilters filter_variant; + // VCF record filters (applied during vcf merging) + //bool filter_by_target; // Filter records based on mets information in target bed info field + //bool hotspot_positions_only; // Output only vcf lines with the infoFlag 'HS' + //bool hotspot_variants_only; // Suppress hotspot reference calls and no-calls from the final output vcf + // tuning parameter for xbias - // float xbias_tune; + //float xbias_tune; float sbias_tune; - BasicFilters filter_snps; - BasicFilters filter_mnp; - BasicFilters filter_hp_indel; - BasicFilters filter_hotspot; + // Filtering parameters that depend on the variant type. + BasicFilters filter_snp = BasicFilters("snp"); + BasicFilters filter_mnp = BasicFilters("mnp"); + BasicFilters filter_hp_indel = BasicFilters("indel"); + BasicFilters filter_hotspot = BasicFilters("hotspot"); + BasicFilters filter_fd_0 = BasicFilters("fd-0"); + BasicFilters filter_fd_5 = BasicFilters("fd-5"); + BasicFilters filter_fd_10 = BasicFilters("fd-10"); ControlCallAndFilters(); void SetOpts(OptArgs &opts, Json::Value& tvc_params); @@ -201,9 +224,10 @@ class ProgramControlSettings { bool do_indel_assembly; + // Directory in ExtendParameters directly bool rich_json_diagnostic; bool minimal_diagnostic; - string json_plot_dir; + bool use_SSE_basecaller; bool suppress_recalibration; @@ -212,25 +236,32 @@ class ProgramControlSettings { bool inputPositionsOnly; bool is_multi_min_allele_freq; - vector snp_multi_min_allele_freq; - vector mnp_multi_min_allele_freq; - vector indel_multi_min_allele_freq; - vector hotspot_multi_min_allele_freq; + vector multi_min_allele_freq; ProgramControlSettings(); void SetOpts(OptArgs &opts, Json::Value & pf_params); void CheckParameterLimits(); }; +struct TvcTagTrimmerParameters : TagTrimmerParameters{ + int indel_func_size_offset = 0; +}; + class ExtendParameters { public: vector bams; string fasta; // -f --fasta-reference string targets; // -t --targets - string outputFile; + + string small_variants_vcf; // small indel output vcf file name + string indel_assembly_vcf; // indel assembly vcf file name + //string merged_vcf; // merged and post processed vcf file name + //string merged_genome_vcf; // merged gvcf file name + string blacklistFile; string variantPriorsFile; string postprocessed_bam; + string json_plot_dir; string basecaller_version; string tmap_version; @@ -239,11 +270,16 @@ class ExtendParameters { bool processInputPositionsOnly; bool trim_ampliseq_primers; + float min_cov_fraction; + int prefixExclusion; // operation parameters + //TODO: Put the Freebayes parameters in a container. bool useDuplicateReads; // -E --use-duplicate-reads int useBestNAlleles; // -n --use-best-n-alleles + int max_alt_num; // Try to break the variant if the number of alt alleles is greater than this value. + int useBestNTotalAlleles; // --use-best-n-total-alleles bool allowIndels; // -I --allow-indels bool allowMNPs; // -X --allow-mnps bool allowComplex; // -X --allow-complex @@ -252,6 +288,7 @@ class ExtendParameters { int min_mapping_qv; // -m --min-mapping-quality float readMaxMismatchFraction; // -z --read-max-mismatch-fraction int read_snp_limit; // -$ --read-snp-limit + int read_mismatch_limit; long double minAltFraction; // -F --min-alternate-fraction long double minIndelAltFraction; // Added by SU to reduce Indel Candidates for Somatic int minAltCount; // -C --min-alternate-count @@ -261,16 +298,17 @@ class ExtendParameters { bool debug; // set if debuglevel >=1 bool multisample; // multisample run - + string candidate_list, black_listed; OptArgs opts; ControlCallAndFilters my_controls; EnsembleEvalTuningParameters my_eval_control; ProgramControlSettings program_flow; - TagTrimmerParameters tag_trimmer_parameters; + TvcTagTrimmerParameters tag_trimmer_parameters; //Input files string outputDir; + string sseMotifsDir; string sseMotifsFileName; bool sseMotifsProvided; @@ -279,11 +317,13 @@ class ExtendParameters { string recal_model_file_name; int recalModelHPThres; + bool output_allele_cigar; string params_meta_name; string params_meta_details; // functions + ExtendParameters() {} ExtendParameters(int argc, char** argv); bool ValidateAndCanonicalizePath(string &path); @@ -291,67 +331,24 @@ class ExtendParameters { void SetFreeBayesParameters(OptArgs &opts, Json::Value& fb_params); void ParametersFromJSON(OptArgs &opts, Json::Value &tvc_params, Json::Value &fb_params, Json::Value ¶ms_meta); void CheckParameterLimits(); + void SetMolecularTagTrimmerOpt(Json::Value& tvc_params); }; -template -bool CheckParameterLowerUpperBound(string identifier ,T ¶meter, T lower_limit, T upper_limit) { - bool is_ok = false; - - //cout << setw(35) << long_name_hyphens << " = " << setw(10) << value << " (integer, " << source << ")" << endl; - cout << "Limit check parameter " << identifier << ": lim. " - << lower_limit << " <= " << parameter << " <= lim. " << upper_limit << "? "; - if (parameter < lower_limit) { - cout << "Using " << identifier << "=" << lower_limit << " instead!"; - parameter = lower_limit; - } - else if (parameter > upper_limit) { - cout << "Using " << identifier << "=" << upper_limit << " instead!"; - parameter = upper_limit; - } - else { - cout << "OK!"; - is_ok = true; - } - cout << endl; - return (is_ok); -} - -template -bool CheckParameterLowerBound(string identifier ,T ¶meter, T lower_limit) { - bool is_ok = false; - cout << "Limit check parameter " << identifier << ": lim. " - << lower_limit << " <= " << parameter << "? "; - if (parameter < lower_limit) { - cout << "Using " << identifier << "=" << lower_limit << " instead!"; - parameter = lower_limit; - } - else { - cout << "OK!"; - is_ok = true; - } - cout << endl; - return (is_ok); +template +string PrintIteratorToString(const MyIter &it_start, const MyIter &it_end, + string left_bracket = "[", string right_bracket = "]", string separation = ", ", string entry_prefix = "") { + string return_str = left_bracket; + MyIter last_it = it_end; + --last_it; + for (MyIter it = it_start; it != it_end; ++it){ + return_str += (entry_prefix + to_string(*it)); + if (it != last_it) + return_str += separation; + } + return_str += right_bracket; + return return_str; } -template -bool CheckParameterUpperBound(string identifier ,T ¶meter, T upper_limit) { - bool is_ok = false; - cout << "Limit check parameter " << identifier << ": " - << parameter << " <= lim. " << upper_limit << "? "; - if (parameter > upper_limit) { - cout << "Using " << identifier << "=" << upper_limit << " instead!"; - parameter = upper_limit; - } - else { - cout << "OK!"; - is_ok = true; - } - cout << endl; - return (is_ok); -} - -bool CheckParameterStringContext(string identifier, string ¶meter, const string &context, const string &default_value); - #endif // EXTENDPARAMETERS_H diff --git a/Analysis/VariantCaller/Bookkeeping/InputStructures.cpp b/Analysis/VariantCaller/Bookkeeping/InputStructures.cpp index 26249fd7..26380c13 100644 --- a/Analysis/VariantCaller/Bookkeeping/InputStructures.cpp +++ b/Analysis/VariantCaller/Bookkeeping/InputStructures.cpp @@ -7,6 +7,7 @@ #include "InputStructures.h" #include "ExtendedReadInfo.h" #include "json/json.h" +#include "MolecularTag.h" InputStructures::InputStructures() { @@ -34,7 +35,7 @@ void InputStructures::Initialize(ExtendParameters ¶meters, const ReferenceRe // now get calibration information, padded to account if nFlows for some bam is large do_recal.ReadRecalibrationFromComments(bam_header,num_flows_by_run_id); // protect against over-flowing nFlows, 0-based - if (parameters.sseMotifsProvided) { + if ((parameters.sseMotifsProvided) && (parameters.my_controls.filter_variant.sseProbThreshold < 1.0)) { cout << "Loading systematic error contexts." << endl; read_error_motifs(parameters.sseMotifsFileName); cout << "Loaded." << endl; @@ -174,7 +175,7 @@ void InputStructures::DetectFlowOrderzAndKeyFromBam(const SamHeader &samHeader){ } // Verbose output - cout << "TVC found a total of " << flow_order_vector.size() << " different flow orders of max flow lengths: "; + cout << "Found a total of " << flow_order_vector.size() << " different flow orders of max flow lengths: "; int iFO=0; for (; iFO<(int)flow_order_vector.size()-1; iFO++) cout << flow_order_vector.at(iFO).num_flows() << ','; @@ -313,7 +314,7 @@ PersistingThreadObjects::PersistingThreadObjects(const InputStructures &global_c #ifdef __SSE3__ if (use_SSE_basecaller) { for (unsigned int iFO=0; iFO < global_context.flow_order_vector.size(); iFO++){ - TreephaserSSE* treephaser_sse = new TreephaserSSE(global_context.flow_order_vector.at(iFO), DPTreephaser::kWindowSizeDefault_); + TreephaserSSE treephaser_sse(global_context.flow_order_vector.at(iFO), DPTreephaser::kWindowSizeDefault_); treephaserSSE_vector.push_back(treephaser_sse); } } diff --git a/Analysis/VariantCaller/Bookkeeping/InputStructures.h b/Analysis/VariantCaller/Bookkeeping/InputStructures.h index 7d7af83b..a8befedb 100644 --- a/Analysis/VariantCaller/Bookkeeping/InputStructures.h +++ b/Analysis/VariantCaller/Bookkeeping/InputStructures.h @@ -39,7 +39,7 @@ class OrderedVCFWriter; class SampleManager; class MetricsManager; class IndelAssembly; - +class MolecularTagManager; // ============================================================================== struct VariantCallerContext { @@ -55,7 +55,7 @@ struct VariantCallerContext { MetricsManager * metrics_manager; //! Keeps track of metrics to output in tvc_metrics.json SampleManager* sample_manager; //! Tracks the sample used in multi-sample analysis IndelAssembly* indel_assembly; //! Outputs the indel_assembly.vcf for long indels - MolecularTagTrimmer* tag_trimmer; //! Manager for molecular tags + MolecularTagManager* mol_tag_manager; //! Manager for molecular tags pthread_mutex_t bam_walker_mutex; //! Mutex for state-altering bam_walker operations pthread_mutex_t read_loading_mutex; //! Mutex for raw read retrieval @@ -79,6 +79,7 @@ struct VariantSpecificParams { min_coverage_override(false), min_coverage(0), min_coverage_each_strand_override(false), min_coverage_each_strand(0), + min_var_coverage_override(false), min_var_coverage(0), min_variant_score_override(false), min_variant_score(0), data_quality_stringency_override(false), data_quality_stringency(0), @@ -90,6 +91,7 @@ struct VariantSpecificParams { filter_insertion_predictions_override(false), filter_insertion_predictions(0), filter_deletion_predictions_override(false), filter_deletion_predictions(0), sse_prob_threshold_override(false), sse_prob_threshold(0), + min_tag_fam_size_override(false), min_tag_fam_size(0), black_strand('.') {} bool min_allele_freq_override; @@ -102,6 +104,8 @@ struct VariantSpecificParams { int min_coverage; bool min_coverage_each_strand_override; int min_coverage_each_strand; + bool min_var_coverage_override; + int min_var_coverage; bool min_variant_score_override; float min_variant_score; bool data_quality_stringency_override; @@ -122,6 +126,8 @@ struct VariantSpecificParams { float filter_deletion_predictions; bool sse_prob_threshold_override; float sse_prob_threshold; + bool min_tag_fam_size_override; + int min_tag_fam_size; char black_strand; }; @@ -197,19 +203,19 @@ class PersistingThreadObjects { PersistingThreadObjects(const InputStructures &global_context); ~PersistingThreadObjects() { -#ifdef __SSE3__ - for (vector::iterator iter = treephaserSSE_vector.begin(); (iter != treephaserSSE_vector.end()); ++iter) { - delete *iter; - *iter = NULL; - } -#endif +//#ifdef __SSE3__ +// for (vector::iterator iter = treephaserSSE_vector.begin(); (iter != treephaserSSE_vector.end()); ++iter) { +// delete *iter; +// *iter = NULL; +// } +//#endif }; //@brief Interface for setting the phasing model parameters void SetModelParameters(const int & flow_order_index, const vector & phase_params) { #ifdef __SSE3__ if (use_SSE_basecaller) - treephaserSSE_vector.at(flow_order_index)->SetModelParameters(phase_params.at(0), phase_params.at(1)); + treephaserSSE_vector.at(flow_order_index).SetModelParameters(phase_params.at(0), phase_params.at(1)); else #endif dpTreephaser_vector.at(flow_order_index).SetModelParameters(phase_params.at(0), phase_params.at(1), phase_params.at(2)); @@ -220,7 +226,7 @@ class PersistingThreadObjects { { #ifdef __SSE3__ if (use_SSE_basecaller) - treephaserSSE_vector.at(flow_order_index)->DisableRecalibration(); + treephaserSSE_vector.at(flow_order_index).DisableRecalibration(); else #endif dpTreephaser_vector.at(flow_order_index).DisableRecalibration(); @@ -231,7 +237,7 @@ class PersistingThreadObjects { { #ifdef __SSE3__ if (use_SSE_basecaller) - return (treephaserSSE_vector.at(flow_order_index)->SetAsBs(As, Bs)); + return (treephaserSSE_vector.at(flow_order_index).SetAsBs(As, Bs)); else #endif return (dpTreephaser_vector.at(flow_order_index).SetAsBs(As, Bs)); @@ -241,7 +247,7 @@ class PersistingThreadObjects { void SolveRead(const int & flow_order_index, BasecallerRead& read, const int & begin_flow, const int & end_flow){ #ifdef __SSE3__ if (use_SSE_basecaller) - treephaserSSE_vector.at(flow_order_index)->SolveRead(read, begin_flow, end_flow); + treephaserSSE_vector.at(flow_order_index).SolveRead(read, begin_flow, end_flow); else #endif dpTreephaser_vector.at(flow_order_index).Solve(read, end_flow, begin_flow); @@ -253,7 +259,7 @@ class PersistingThreadObjects { Realigner realigner; // realignment tool vector dpTreephaser_vector; // c++ treephaser #ifdef __SSE3__ - vector treephaserSSE_vector; // vectorized treephaser + vector treephaserSSE_vector; // vectorized treephaser #endif diff --git a/Analysis/VariantCaller/Bookkeeping/VcfFormat.cpp b/Analysis/VariantCaller/Bookkeeping/VcfFormat.cpp index a2a1e0c3..cc9c60c8 100644 --- a/Analysis/VariantCaller/Bookkeeping/VcfFormat.cpp +++ b/Analysis/VariantCaller/Bookkeeping/VcfFormat.cpp @@ -23,7 +23,7 @@ string dateStr() } -string get_time_iso_string(time_t time) +string tvc_get_time_iso_string(time_t time) { char time_buffer[1024]; strftime(time_buffer, 1024, "%Y-%m-%dT%H:%M:%S", localtime(&time)); @@ -31,13 +31,13 @@ string get_time_iso_string(time_t time) } -string getVCFHeader(const ExtendParameters *parameters, ReferenceReader& ref_reader, const vector& sample_list, int primary_sample) +string getVCFHeader(const ExtendParameters *parameters, ReferenceReader& ref_reader, const vector& sample_list, int primary_sample, bool use_molecular_tag) { stringstream headerss; headerss << "##fileformat=VCFv4.1" << endl << "##fileDate=" << dateStr() << endl - << "##fileUTCtime=" << get_time_iso_string(time(NULL)) << endl + << "##fileUTCtime=" << tvc_get_time_iso_string(time(NULL)) << endl << "##source=\"tvc " << IonVersion::GetVersion() << "-" << IonVersion::GetRelease() << " (" << IonVersion::GetGitHash() << ") - Torrent Variant Caller\"" << endl; if (not parameters->params_meta_name.empty()) @@ -72,6 +72,7 @@ string getVCFHeader(const ExtendParameters *parameters, ReferenceReader& ref_rea string tmp(first+1); headerss << "##maskVersion=" << tmp << endl; } + fclose(fp); } string chr_name; long chr_size; @@ -90,12 +91,6 @@ string getVCFHeader(const ExtendParameters *parameters, ReferenceReader& ref_rea << "##INFO=" << endl << "##INFO=" << endl << "##INFO=" << endl - - << "##INFO=" << endl - << "##INFO=" << endl - << "##INFO=" << endl - << "##INFO=" << endl - << "##INFO=" << endl << "##INFO=" << endl << "##INFO=" << endl @@ -116,7 +111,6 @@ string getVCFHeader(const ExtendParameters *parameters, ReferenceReader& ref_rea << "##INFO=" << endl << "##INFO=" << endl // << "##INFO=" << endl - // << "##INFO=" << endl; // << "##INFO=" << endl @@ -130,18 +124,55 @@ string getVCFHeader(const ExtendParameters *parameters, ReferenceReader& ref_rea << "##INFO=" << endl << "##INFO=" << endl << "##INFO=" << endl - << "##INFO=" << endl + << "##INFO=" << endl << "##INFO=" << endl << "##INFO=" << endl << "##INFO=" << endl << "##INFO=" << endl << "##INFO=X)+0.5P(Y=X)\">" << endl << "##INFO=" << endl - << "##INFO=" << endl; + << "##INFO=" << endl + << "##INFO=" << endl; - if(parameters->my_controls.use_lod_filter){ + // If we want to output multiple min-allele-freq + if (parameters->program_flow.is_multi_min_allele_freq){ + int multi_min_allele_freq_size = (int) parameters->program_flow.multi_min_allele_freq.size(); + headerss + << "##INFO=program_flow.multi_min_allele_freq[i_maf] << (i_maf < (multi_min_allele_freq_size - 1) ? ",": ""); + } + headerss << ").\">" << endl + << "##INFO=" << endl + << "##INFO=" << endl; + } + if (parameters->output_allele_cigar) { + headerss << "##INFO=" << endl; + } + if (parameters->my_controls.use_lod_filter){ headerss << "##INFO=" << endl; } + if (use_molecular_tag){ + headerss << "##INFO=" << endl + << "##INFO=" << endl + << "##INFO=" << endl + << "##INFO=" << endl + << "##INFO=" << endl + << "##INFO=" << endl; + } + if (parameters->my_controls.report_ppa){ + headerss << "##INFO=" << endl; + } + if (parameters->my_controls.disable_filters){ + headerss << "##INFO=" << endl + << "##INFO=" << endl + << "##INFO=" << endl + << "##INFO=" << endl + << "##INFO=" << endl + << "##INFO=" << endl + << "##INFO=" << endl; + } + headerss << "##FILTER=" << endl; @@ -149,15 +180,16 @@ string getVCFHeader(const ExtendParameters *parameters, ReferenceReader& ref_rea << "##FORMAT=" << endl << "##FORMAT=" << endl << "##FORMAT=" << endl + << "##FORMAT=" << endl; - << "##FORMAT=" << endl - - << "##FORMAT=" << endl - << "##FORMAT=" << endl - << "##FORMAT=" << endl - << "##FORMAT=" << endl + if(use_molecular_tag){ + headerss << "##FORMAT=" << endl + << "##FORMAT=" << endl + << "##FORMAT=" << endl + << "##FORMAT=" << endl; + } - << "##FORMAT=" << endl + headerss << "##FORMAT=" << endl << "##FORMAT=" << endl << "##FORMAT=" << endl << "##FORMAT=" << endl @@ -170,43 +202,7 @@ string getVCFHeader(const ExtendParameters *parameters, ReferenceReader& ref_rea << "##FORMAT=" << endl << "##FORMAT=" << endl << "##FORMAT=" << endl - << "##FORMAT=" << endl - << "##FORMAT=" << endl; - - // If we want to output multiple min-allele-freq - if(parameters->program_flow.is_multi_min_allele_freq){ - string multi_min_allele_freq_size = "?"; - string snp_multi_min_allele_freq_size = convertToString(parameters->program_flow.snp_multi_min_allele_freq.size()); - string mnp_multi_min_allele_freq_size = convertToString(parameters->program_flow.mnp_multi_min_allele_freq.size()); - string indel_multi_min_allele_freq_size = convertToString(parameters->program_flow.indel_multi_min_allele_freq.size()); - string hotspot_multi_min_allele_freq_size = convertToString(parameters->program_flow.hotspot_multi_min_allele_freq.size()); - // the union of all var types - headerss<< "##FORMAT=" << endl - << "##FORMAT=" << endl - << "##FORMAT=" << endl - << "##FORMAT=" << endl - // for snp - << "##FORMAT=" << endl - << "##FORMAT=" << endl - << "##FORMAT=" << endl - << "##FORMAT=" << endl - // for mnp - << "##FORMAT=" << endl - << "##FORMAT=" << endl - << "##FORMAT=" << endl - << "##FORMAT=" << endl - // for indel - << "##FORMAT=" << endl - << "##FORMAT=" << endl - << "##FORMAT=" << endl - << "##FORMAT=" << endl - // for hotspot - << "##FORMAT=" << endl - << "##FORMAT=" << endl - << "##FORMAT=" << endl - << "##FORMAT=" << endl; - } - + << "##FORMAT=" << endl; headerss << "#CHROM\tPOS\tID\tREF\tALT\tQUAL\tFILTER\tINFO\tFORMAT"; // Ensure primary sample is always in the first column (IR req) @@ -235,177 +231,51 @@ void ClearVal(vcf::Variant &var, const char *clear_me){ //clear all the info tags, in case of a HotSpot VCF react Info tags might contain prior values void clearInfoTags(vcf::Variant &var) { - map >::iterator it; - - it = var.info.find("RO"); - if (it != var.info.end()) - var.info["RO"].clear(); - - it = var.info.find("AO"); - if (it != var.info.end()) - var.info["AO"].clear(); - - it = var.info.find("MDP"); - if (it != var.info.end()) - var.info["MDP"].clear(); - - it = var.info.find("MAO"); - if (it != var.info.end()) - var.info["MAO"].clear(); - - it = var.info.find("MRO"); - if (it != var.info.end()) - var.info["MRO"].clear(); - - it = var.info.find("MAF"); - if (it != var.info.end()) - var.info["MAF"].clear(); - - it = var.info.find("SAF"); - if (it != var.info.end()) - var.info["SAF"].clear(); - - it = var.info.find("SAR"); - if (it != var.info.end()) - var.info["SAR"].clear(); - - it = var.info.find("SRF"); - if (it != var.info.end()) - var.info["SRF"].clear(); - - it = var.info.find("SRR"); - if (it != var.info.end()) - var.info["SRR"].clear(); - - it = var.info.find("DP"); - if (it != var.info.end()) - var.info["DP"].clear(); - - - it = var.info.find("RBI"); - if (it != var.info.end()) - var.info["RBI"].clear(); - - it = var.info.find("HRUN"); - if (it != var.info.end()) - var.info["HRUN"].clear(); - - - it = var.info.find("MLLD"); - if (it != var.info.end()) - var.info["MLLD"].clear(); - - //SSE compute - ClearVal(var,"SSSB"); - ClearVal(var,"SSEN"); - ClearVal(var,"SSEP"); - - it = var.info.find("STB"); - if (it != var.info.end()) - var.info["STB"].clear(); - - it = var.info.find("STBP"); - if (it != var.info.end()) - var.info["STBP"].clear(); - - it = var.info.find("PBP"); - if (it != var.info.end()) - var.info["PBP"].clear(); - - it = var.info.find("LOD"); - if (it != var.info.end()) - var.info["LOD"].clear(); - -/* it = var.info.find("SXB"); - if (it != var.info.end()) - var.info["SXB"].clear();*/ - - ClearVal(var,"FDP"); - ClearVal(var,"FRO"); - ClearVal(var,"FAO"); - //ClearVal(var,"JUNK"); - ClearVal(var,"FSRF"); - ClearVal(var,"FSRR"); - ClearVal(var,"FSAF"); - ClearVal(var,"FSAR"); - ClearVal(var,"FXX"); - ClearVal(var,"QD"); + const vector tag_to_clear = + {"RO", "AO", "MDP", "MAO", "MRO", "MAF", "SAF", "SAR", "SRF", "SRR", + "DP", "RBI", "HRUN", "SSSB", "SSEN", "SSEP", "STB", "STBP", "PBP", "PB", + "FDP", "FRO", "FAO", "FSRF", "FSRR", "FSAF", "FSAR", "FXX", "QD", "TGSM", + "PPA", "VFSH", "MUQUAL", "MUGT", "MUGQ", "MLLD", "LOD"}; + for (vector::const_iterator tag_it = tag_to_clear.begin(); tag_it != tag_to_clear.end(); ++tag_it) + ClearVal(var, tag_it->c_str()); } -void NullInfoFields(vcf::Variant &var, bool use_position_bias){ - clearInfoTags(var); - for (vector::iterator I = var.alt.begin(); I != var.alt.end(); ++I) { - var.info["AO"].push_back(convertToString(0)); - var.info["MAF"].push_back(convertToString(0)); - var.info["MAO"].push_back(convertToString(0)); - var.info["SAF"].push_back(convertToString(0)); - var.info["SAR"].push_back(convertToString(0)); - var.info["FAO"].push_back(convertToString(0)); - var.info["AF"].push_back(convertToString(0)); - var.info["FSAF"].push_back(convertToString(0)); - var.info["FSAR"].push_back(convertToString(0)); - var.info["HRUN"].push_back(convertToString(0)); - var.info["RBI"].push_back(convertToString(0)); - var.info["FWDB"].push_back(convertToString(0)); - var.info["REVB"].push_back(convertToString(0)); - var.info["REFB"].push_back(convertToString(0)); - var.info["VARB"].push_back(convertToString(0)); - var.info["SSSB"].push_back(convertToString(0)); - var.info["SSEN"].push_back(convertToString(0)); - var.info["SSEP"].push_back(convertToString(0)); - var.info["STB"].push_back(convertToString(0)); - var.info["STBP"].push_back(convertToString(0)); - if(use_position_bias) { - var.info["PB"].push_back(convertToString(0.5f)); - var.info["PBP"].push_back(convertToString(1.0f)); - } - var.info["MLLD"].push_back(convertToString(0)); - } - var.info["DP"].push_back(convertToString(0)); - var.info["RO"].push_back(convertToString(0)); - var.info["MDP"].push_back(convertToString(0)); - var.info["MRO"].push_back(convertToString(0)); - var.info["SRF"].push_back(convertToString(0)); - var.info["SRR"].push_back(convertToString(0)); - - var.info["FDP"].push_back(convertToString(0)); - var.info["FRO"].push_back(convertToString(0)); - var.info["FSRF"].push_back(convertToString(0)); - var.info["FSRR"].push_back(convertToString(0)); - var.info["FXX"].push_back(convertToString(0.0)); - var.info["QD"].push_back(convertToString(0.0)); +void NullInfoFields(vcf::Variant &var, bool use_position_bias, bool use_molecular_tag){ + int num_alt = (int) var.alt.size(); + clearInfoTags(var); + const vector num_alt_tags_to_zero = + {"AO", "SAF", "SAR", "AF", "FSAF", "FSAR", "HRUN", "RBI", + "FWDB", "REVB", "REFB", "VARB", "SSSB", "SSEN", "SSEP", "MLLD", "AF"}; + for (vector::const_iterator tag_it = num_alt_tags_to_zero.begin(); tag_it != num_alt_tags_to_zero.end(); ++tag_it){ + var.info[*tag_it] = vector(num_alt, "0"); + } + var.info["STB"] = vector(num_alt, "0.5"); + var.info["STBP"] = vector(num_alt, "1"); + + const vector tags_to_zero = + {"DP", "RO", "SRF", "SRR", "FDP", "FRO", "FSRF", "FSRR", "FXX", "QD"}; + for (vector::const_iterator tag_it = tags_to_zero.begin(); tag_it != tags_to_zero.end(); ++tag_it){ + var.info[*tag_it] = {"0"}; + } + if (use_position_bias) { + var.info["PB"] = vector(num_alt, "0.5"); + var.info["PBP"] = vector(num_alt, "1"); + } + if (use_molecular_tag) { + var.info["MDP"] = {"0"}; + var.info["MRO"] = {"0"}; + var.info["MAO"] = vector(num_alt, "0"); + var.info["MAF"] = vector(num_alt, "0"); + } } // set up format string void SetUpFormatString(vcf::Variant &var) { - var.format.clear(); - var.format.push_back("GT"); - var.format.push_back("GQ"); - // XXX - var.format.push_back("MDP"); - var.format.push_back("MRO"); - var.format.push_back("MAO"); - var.format.push_back("MAF"); - var.format.push_back("DP"); - var.format.push_back("FDP"); - var.format.push_back("RO"); - var.format.push_back("FRO"); - var.format.push_back("AO"); - var.format.push_back("FAO"); - //var.format.push_back("JUNK"); - var.format.push_back("AF"); - var.format.push_back("SAR"); - var.format.push_back("SAF"); - var.format.push_back("SRF"); - var.format.push_back("SRR"); - var.format.push_back("FSAR"); - var.format.push_back("FSAF"); - var.format.push_back("FSRF"); - var.format.push_back("FSRR"); - var.format.push_back("QT"); + var.format = {"GT", "GQ", "MDP", "MRO", "MAO", "MAF", "DP", + "FDP", "RO", "FRO", "AO", "FAO", "AF", "SAR", + "SAF", "SRF", "SRR", "FSAR", "FSAF", "FSRF", "FSRR"}; } - int CalculateWeightOfVariant(vcf::Variant ¤t_variant){ map >::iterator it; @@ -454,18 +324,13 @@ float RetrieveQualityTagValue(vcf::Variant ¤t_variant, const string &tag_w // XXX void NullFilterReason(vcf::Variant &candidate_variant, const string &sample_name){ - ClearVal(candidate_variant, "FR"); - for (vector::iterator I = candidate_variant.alt.begin(); I != candidate_variant.alt.end(); ++I) { - candidate_variant.info["FR"].push_back("."); - } + candidate_variant.info["FR"] = vector(candidate_variant.alt.size(), "."); } void AddFilterReason(vcf::Variant &candidate_variant, string &additional_reason, const string &sample_name){ - int alt_allele_index = 0; - for (vector::iterator I = candidate_variant.alt.begin(); I != candidate_variant.alt.end(); ++I) { - AddFilterReason(candidate_variant, additional_reason, sample_name, alt_allele_index); - alt_allele_index++; - } + for (unsigned int alt_allele_index = 0; alt_allele_index < candidate_variant.alt.size(); ++alt_allele_index){ + AddFilterReason(candidate_variant, additional_reason, sample_name, alt_allele_index); + } } void AddFilterReason(vcf::Variant &candidate_variant, string &additional_reason, const string &sample_name, unsigned int alt_allele_index){ @@ -480,89 +345,44 @@ void AddInfoReason(vcf::Variant &candidate_variant, string &additional_reason, c } // if, for example, missing data -void NullGenotypeAllSamples(vcf::Variant & candidate_variant) +void NullGenotypeAllSamples(vcf::Variant & candidate_variant, bool use_molecular_tag) { vector& sampleNames = candidate_variant.sampleNames; - - for (vector::iterator its = sampleNames.begin(); its != sampleNames.end(); ++its) { - string& sampleName = *its; - map >& sampleOutput = candidate_variant.samples[sampleName]; - sampleOutput["GT"].clear(); - sampleOutput["GQ"].clear(); - sampleOutput["MDP"].clear(); - sampleOutput["MRO"].clear(); - sampleOutput["MAO"].clear(); - sampleOutput["MAF"].clear(); - sampleOutput["FDP"].clear(); - sampleOutput["FRO"].clear(); - sampleOutput["FSRF"].clear(); - sampleOutput["FSRR"].clear(); - sampleOutput["FAO"].clear(); - sampleOutput["AF"].clear(); - sampleOutput["FSAF"].clear(); - sampleOutput["FSAR"].clear(); - sampleOutput["GT"].push_back("./."); - sampleOutput["GQ"].push_back(convertToString(0)); - sampleOutput["MDP"].push_back(convertToString(0)); - sampleOutput["MRO"].push_back(convertToString(0)); - sampleOutput["FDP"].push_back(convertToString(0)); - sampleOutput["FRO"].push_back(convertToString(0)); - sampleOutput["FSRF"].push_back(convertToString(0)); - sampleOutput["FSRR"].push_back(convertToString(0)); - for (vector::iterator I = candidate_variant.alt.begin(); I != candidate_variant.alt.end(); ++I) { - sampleOutput["MAO"].push_back(convertToString(0)); - sampleOutput["MAF"].push_back(convertToString(0)); - sampleOutput["FAO"].push_back(convertToString(0)); - sampleOutput["AF"].push_back(convertToString(0)); - sampleOutput["FSAF"].push_back(convertToString(0)); - sampleOutput["FSAR"].push_back(convertToString(0)); + const vector keys_to_zero = {"GQ", "FDP", "FRO", "FSRF", "FSRR"}; + const vector keys_to_zeros_of_alt = {"FAO", "AF", "FSAF", "FSAR"}; + unsigned int num_alt = candidate_variant.alt.size(); + for (vector::iterator sample_it = sampleNames.begin(); sample_it != sampleNames.end(); ++sample_it) { + map >& sampleOutput = candidate_variant.samples[*sample_it]; + + for (vector::const_iterator key_it = keys_to_zero.begin(); key_it != keys_to_zero.end(); ++key_it){ + sampleOutput[*key_it] = {"0"}; } + for (vector::const_iterator key_it = keys_to_zeros_of_alt.begin(); key_it != keys_to_zeros_of_alt.end(); ++key_it){ + sampleOutput[*key_it] = vector(num_alt, "0"); + } + sampleOutput["GT"] = {"./."}; - sampleOutput["QT"].clear(); - sampleOutput["QT"].push_back("."); - - // for multi-min-allele-freq - vector tags_for_multi_min_allele_freq = {"MUAF", "MUQUAL", "MUGQ", "MUGT", - "SMAF", "SMQUAL", "SMGQ", "SMGT", - "MMAF", "MMQUAL", "MMGQ", "MMGT", - "IMAF", "IMQUAL", "IMGQ", "IMGT", - "HMAF", "HMQUAL", "HMGQ", "HMGT"}; - map >::iterator it; - for(unsigned int i_tag = 0; i_tag < tags_for_multi_min_allele_freq.size(); ++i_tag){ - it = sampleOutput.find(tags_for_multi_min_allele_freq[i_tag]); - if (it != sampleOutput.end()){ - it->second.clear(); - it->second.push_back("."); - } - } + if (use_molecular_tag){ + sampleOutput["MDP"] = {"0"}; + sampleOutput["MRO"] = {"0"}; + sampleOutput["MAO"] = vector(num_alt, "0"); + sampleOutput["MAF"] = vector(num_alt, "0"); + } } } void OverwriteGenotypeForOneSample(vcf::Variant &candidate_variant, const string &my_sample_name, string &my_genotype, float genotype_quality){ - // will create entry if one does not exist - - map >& sampleOutput = candidate_variant.samples[my_sample_name]; - // clear existing values - map >::iterator it; - it = sampleOutput.find("GT"); - if (it != sampleOutput.end()) sampleOutput["GT"].clear(); - it = sampleOutput.find("GQ"); - if (it != sampleOutput.end()) sampleOutput["GQ"].clear(); - - - sampleOutput["GT"].push_back(my_genotype); - // genotype quality should be an "int" - //cout << "Storing Genotype = " << my_genotype << endl; - sampleOutput["GQ"].push_back(convertToString((int)genotype_quality)); - + map >& sampleOutput = candidate_variant.samples[my_sample_name]; + sampleOutput["GT"] = {my_genotype}; + sampleOutput["GQ"] = {convertToString((int) genotype_quality)}; } void DetectAndSetFilteredGenotype(vcf::Variant &candidate_variant, map& variant_quality, const string &sample_name){ -if (candidate_variant.isFiltered){ - string no_call_genotype = "./."; - float original_quality = variant_quality[sample_name]; - OverwriteGenotypeForOneSample(candidate_variant, sample_name, no_call_genotype, original_quality); -} + if (candidate_variant.isFiltered){ + string no_call_genotype = "./."; + float original_quality = variant_quality[sample_name]; + OverwriteGenotypeForOneSample(candidate_variant, sample_name, no_call_genotype, original_quality); + } } @@ -571,37 +391,24 @@ void StoreGenotypeForOneSample(vcf::Variant &candidate_variant, const string &sa if (multisample) { map >& sampleOutput = candidate_variant.samples[sample_name]; - sampleOutput["GT"].clear(); - sampleOutput["GT"].push_back(my_genotype); - //cout << "Storing Genotype = " << my_genotype << endl; - sampleOutput["GQ"].clear(); - sampleOutput["GQ"].push_back(convertToString((int)genotype_quality)); + sampleOutput["GT"] = {my_genotype}; + sampleOutput["GQ"] = {convertToString((int) genotype_quality)}; } else { - for (vector::iterator its = sampleNames.begin(); its != sampleNames.end(); ++its) { - string& sampleName = *its; - //cout << "VariantAssist: SampleName = " << sampleName << " my_sample = " << my_sample_name << endl; - map >& sampleOutput = candidate_variant.samples[sampleName]; - map >::iterator it; - it = sampleOutput.find("GT"); - if (it != sampleOutput.end()) sampleOutput["GT"].clear(); - it = sampleOutput.find("GQ"); - if (it != sampleOutput.end()) sampleOutput["GQ"].clear(); - - if (sampleName.compare(sample_name) == 0) { //sample of interest - //cout << "isNocall " << isNoCall << " genotype = " << my_genotype << endl; - - // if no-call, will reset this entry as a final step, but until then, give me my genotype - sampleOutput["GT"].push_back(my_genotype); - //cout << "Storing Genotype = " << my_genotype << endl; - sampleOutput["GQ"].push_back(convertToString((int)genotype_quality)); - - } else { //for all other samples in BAM file just make a no-call at this point. - sampleOutput["GT"].push_back("./."); - sampleOutput["GQ"].push_back(convertToString(0)); - } - //cout <<"VariantAssist: total genotypes = " << sampleOutput["GT"].size() << endl; + for (vector::iterator its = sampleNames.begin(); its != sampleNames.end(); ++its) { + string& sampleName = *its; + map >& sampleOutput = candidate_variant.samples[sampleName]; + if (sampleName.compare(sample_name) == 0) { //sample of interest + // if no-call, will reset this entry as a final step, but until then, give me my genotype + sampleOutput["GT"] = {my_genotype}; + //cout << "Storing Genotype = " << my_genotype << endl; + sampleOutput["GQ"] = {convertToString((int) genotype_quality)}; } + else{ //for all other samples in BAM file just make a no-call at this point. + sampleOutput["GT"] = {"./."}; + sampleOutput["GQ"] = {"0"}; + } + } } } @@ -616,7 +423,6 @@ void SetFilteredStatus(vcf::Variant &candidate_variant, bool isFiltered) { candidate_variant.filter = "PASS"; candidate_variant.isFiltered = false; } - } @@ -647,6 +453,14 @@ void AdjustFDPForRemovedAlleles(vcf::Variant &candidate_variant, int filtered_al map >& sampleOutput = candidate_variant.samples[sampleName]; sampleOutput["FDP"].clear(); sampleOutput["FDP"].push_back(convertToString(total_depth)); + it = candidate_variant.info.find("MDP"); + if (it != candidate_variant.info.end()){ + candidate_variant.info["MDP"] = candidate_variant.info["FDP"]; + } + it = sampleOutput.find("MDP"); + if (it != sampleOutput.end()){ + sampleOutput["MDP"] = sampleOutput["FDP"]; + } } } diff --git a/Analysis/VariantCaller/Bookkeeping/VcfFormat.h b/Analysis/VariantCaller/Bookkeeping/VcfFormat.h index 8ca0b60a..4daaa70d 100644 --- a/Analysis/VariantCaller/Bookkeeping/VcfFormat.h +++ b/Analysis/VariantCaller/Bookkeeping/VcfFormat.h @@ -33,9 +33,10 @@ using namespace std; // forward declaration class ExtendParameters; -string getVCFHeader(const ExtendParameters *parameters, ReferenceReader& ref_reader, const vector& sample_list, int primary_sample = 0); +string tvc_get_time_iso_string(time_t time); +string getVCFHeader(const ExtendParameters *parameters, ReferenceReader& ref_reader, const vector& sample_list, int primary_sample = 0, bool use_molecular_tag = false); void clearInfoTags(vcf::Variant &var); -void NullInfoFields(vcf::Variant &var, bool use_position_bias); +void NullInfoFields(vcf::Variant &var, bool use_position_bias, bool use_molecular_tag); void SetUpFormatString(vcf::Variant &var); int CalculateWeightOfVariant(vcf::Variant ¤t_variant); void ClearVal(vcf::Variant &var, const char *clear_me); @@ -45,7 +46,7 @@ float RetrieveQualityTagValue(vcf::Variant ¤t_variant, const string &tag_w // double-star pointer here void SetFilteredStatus(vcf::Variant & candidate_variant, bool isFiltered); void StoreGenotypeForOneSample(vcf::Variant & candidate_variant, const string &my_sample_name, string &my_genotype, float genotype_quality, bool multisample); -void NullGenotypeAllSamples(vcf::Variant & candidate_variant); +void NullGenotypeAllSamples(vcf::Variant & candidate_variant, bool use_molecular_tag); void OverwriteGenotypeForOneSample(vcf::Variant & candidate_variant, const string &my_sample_name, string &my_genotype, float genotype_quality); void NullFilterReason(vcf::Variant &candidate_variant, const string &sample_name); void AddFilterReason(vcf::Variant &candidate_variant, string &additional_reason, const string &sample_name); diff --git a/Analysis/VariantCaller/Consensus/Consensus.cpp b/Analysis/VariantCaller/Consensus/Consensus.cpp new file mode 100644 index 00000000..e4eada13 --- /dev/null +++ b/Analysis/VariantCaller/Consensus/Consensus.cpp @@ -0,0 +1,568 @@ +/* Copyright (C) 2016 Thermo Fisher Scientific, All Rights Reserved */ + +//! @file Consensus.cpp +//! @ingroup Consensus +//! @brief Generate flowspace consensus bam file + +#include +#include +#include +#include +#include + +#include "ConsensusParameters.h" +#include "FlowSpaceConsensus.h" +#include "InputStructures.h" +#include "ReferenceReader.h" +#include "OrderedVCFWriter.h" +#include "BAMWalkerEngine.h" +#include "SampleManager.h" +#include "ExtendedReadInfo.h" +#include "TargetsManager.h" +#include "HotspotReader.h" +#include "MetricsManager.h" +#include "IonVersion.h" +#include "MolecularTag.h" +#include "IndelAssembly/IndelAssembly.h" + +#include "json/json.h" +#include "VcfFormat.h" + + +using namespace std; + +// TODO: get rid of global variables! +pthread_mutex_t mutexbam; + +// ---------------------------------------------------------------- +namespace consensus{ +void TheSilenceOfTheArmadillos(ofstream &null_ostream) { + // Disable armadillo warning messages. + arma::set_stream_err1(null_ostream); + arma::set_stream_err2(null_ostream); +} +}; +// ---------------------------------------------------------------- + +struct ConsensusContext : VariantCallerContext { + ConsensusBAMWalkerEngine* consensus_bam_walker; + ConsensusParameters* consensus_parameters; + pthread_mutex_t read_filter_mutex; +}; + +// ---------------------------------------------------------------- +//! @brief Write startup info to json structure. +void DumpStartingStateOfConsensus (int argc, char *argv[], time_t analysis_start_time, Json::Value &json) +{ + char my_host_name[128] = { 0 }; + gethostname (my_host_name, 128); + string command_line = argv[0]; + for (int i = 1; i < argc; i++) { + command_line += " "; + command_line += argv[i]; + } + + json["host_name"] = my_host_name; + json["start_time"] = tvc_get_time_iso_string(analysis_start_time); + json["version"] = IonVersion::GetVersion() + "." + IonVersion::GetRelease(); + json["git_hash"] = IonVersion::GetGitHash(); + json["build_number"] = IonVersion::GetBuildNum(); + json["command_line"] = command_line; +} + +// ---------------------------------------------------------------- + +void SaveJson(const Json::Value & json, const string& filename_json) +{ + ofstream out(filename_json.c_str(), ios::out); + if (out.good()) + out << json.toStyledString(); + else + cerr << "Unable to write JSON file " << filename_json; +} +// ---------------------------------------------------------------- + +void * FlowSpaceConsensusWorker(void *input); + +// ---------------------------------------------------------------- +int ConsensusMain(int argc, char* argv[]) { + pthread_mutex_init(&mutexbam, NULL); + + printf("consensus %s-%s (%s): Generate a consensus bam file by re-basecalling reads that are flow-synchronized\n\n", + IonVersion::GetVersion().c_str(), IonVersion::GetRelease().c_str(), IonVersion::GetGitHash().c_str()); + + // stolen from "Analysis" to silence error messages from Armadillo library + ofstream null_ostream("/dev/null"); // must stay live for entire scope, or crash when writing + consensus::TheSilenceOfTheArmadillos(null_ostream); + + time_t program_start_time = time(NULL); + Json::Value consensus_json(Json::objectValue); + DumpStartingStateOfConsensus (argc, argv, program_start_time, consensus_json["consensus"]); + + // Initialize parameters + ConsensusParameters parameters(argc, argv); + + mkdir(parameters.outputDir.c_str(), S_IRWXU | S_IRWXG | S_IROTH | S_IXOTH); + parameters.program_flow.do_indel_assembly = false; + + // Initialize reference reader + ReferenceReader ref_reader; + ref_reader.Initialize(parameters.fasta); + + // Initialize targets manager + TargetsManager targets_manager; + targets_manager.Initialize(ref_reader, parameters.targets, parameters.min_cov_fraction, parameters.trim_ampliseq_primers); + + // Initialize consens bam walker engine + ConsensusBAMWalkerEngine bam_walker; + try { + bam_walker.Initialize(ref_reader, targets_manager, parameters.bams, parameters.postprocessed_bam, parameters.prefixExclusion, parameters.consensus_bam); + bam_walker.GetProgramVersions(parameters.basecaller_version, parameters.tmap_version); + } + catch(...){ + cerr << "Cannot open input-bam. " << endl; + exit(1); + } + + // Initialize sample manager + SampleManager sample_manager; + sample_manager.Initialize(bam_walker.GetBamHeader(), parameters.sampleName, parameters.force_sample_name, parameters.multisample); + // No multi-sample analysis yet. + + // Initialize global_context + InputStructures global_context; + global_context.Initialize(parameters, ref_reader, bam_walker.GetBamHeader()); + + // Initialize tag trimmer + MolecularTagTrimmer tag_trimmer; + tag_trimmer.InitializeFromSamHeader(parameters.tag_trimmer_parameters, bam_walker.GetBamHeader()); + + cout << "consensus: Generating flow-space consensus bam "<< (tag_trimmer.HaveTags()? "with" : "without") << " molecular tags."<< endl; + if (parameters.skip_consensus){ + cout << "consensus: Skip the calculation of consensus. Output targets_depth.txt only." << endl; + } + + // Initialize molecular tag manager + MolecularTagManager mol_tag_manager; + mol_tag_manager.Initialize(&tag_trimmer, &sample_manager); + + + OrderedVCFWriter vcf_writer; + + OrderedBAMWriter bam_writer; + + HotspotReader hotspot_reader; + + // set up producer of variants + AlleleParser candidate_generator(parameters, ref_reader, sample_manager, vcf_writer, hotspot_reader); + + ConsensusContext vc; + vc.ref_reader = &ref_reader; + vc.targets_manager = &targets_manager; + vc.bam_walker = &bam_walker; + vc.consensus_bam_walker = &bam_walker; + vc.parameters = ¶meters; + vc.consensus_parameters = ¶meters; + vc.global_context = &global_context; + vc.candidate_generator = &candidate_generator; + vc.vcf_writer = NULL; + vc.bam_writer = &bam_writer; + vc.metrics_manager = NULL; + vc.sample_manager = &sample_manager; + vc.mol_tag_manager = &mol_tag_manager; + + pthread_mutex_init(&vc.candidate_generation_mutex, NULL); + pthread_mutex_init(&vc.read_loading_mutex, NULL); + pthread_mutex_init(&vc.bam_walker_mutex, NULL); + pthread_mutex_init(&vc.read_removal_mutex, NULL); + pthread_mutex_init(&vc.read_filter_mutex, NULL); + pthread_cond_init(&vc.memory_contention_cond, NULL); + pthread_cond_init(&vc.alignment_tail_cond, NULL); + + //vc.bam_walker->openDepth(parameters.outputDir + "/depth.txt"); + vc.candidate_counter = 0; + vc.dot_time = time(NULL) + 30; + //vc.candidate_dot = 0; + + pthread_t worker_id[parameters.program_flow.nThreads]; + for (int worker = 0; worker < parameters.program_flow.nThreads; worker++){ + if (pthread_create(&worker_id[worker], NULL, FlowSpaceConsensusWorker, &vc)) { + printf("*Error* - problem starting thread\n"); + exit(-1); + } + } + for (int worker = 0; worker < parameters.program_flow.nThreads; worker++) + { pthread_join(worker_id[worker], NULL); } + + pthread_mutex_destroy(&vc.candidate_generation_mutex); + pthread_mutex_destroy(&vc.read_loading_mutex); + pthread_mutex_destroy(&vc.bam_walker_mutex); + pthread_mutex_destroy(&vc.read_removal_mutex); + pthread_mutex_destroy(&vc.read_filter_mutex); + + pthread_cond_destroy(&vc.memory_contention_cond); + pthread_cond_destroy(&vc.alignment_tail_cond); + + vector::iterator depth_target = targets_manager.merged.begin(); + Alignment* save_list = vc.bam_writer->process_new_entries(vc.bam_walker->alignments_first_); + //vc.bam_walker->SaveAlignments(save_list, vc, depth_target); + vc.bam_walker->FinishReadRemovalTask(save_list, -1); + save_list = vc.bam_writer->flush(); + //vc.bam_walker->SaveAlignments(save_list, vc, depth_target); + vc.bam_walker->FinishReadRemovalTask(save_list, -1); + + vcf_writer.Close(); + bam_walker.Close(); + + // write the target coverage txt + vc.targets_manager->WriteTargetsCoverage(parameters.outputDir + "/targets_depth.txt" , *vc.ref_reader); + + pthread_mutex_destroy(&mutexbam); + + // Determine the most frequent tmap program group for alignment + if (not parameters.skip_consensus){ + SamProgram most_popular_tmap_pg; + if (not bam_walker.GetMostPopularTmap(most_popular_tmap_pg)){ + cerr <<"ERROR: Fail to get tmap program group from the input bam header!" << endl; + exit(-1); + } + consensus_json["tmap"]["command_line"] = most_popular_tmap_pg.CommandLine; + } + + time_t program_end_time = time(NULL); + consensus_json["consensus"]["end_time"] = tvc_get_time_iso_string(program_end_time); + consensus_json["consensus"]["total_duration"] = (Json::Int)difftime(program_end_time,program_start_time); + + cerr << endl; + cout << endl; + cout << "[consensus] Processing time: " << (program_end_time-program_start_time) << " seconds." << endl << endl; + + SaveJson(consensus_json, parameters.outputDir + "/consensus.json"); + return 0; +} + +// ---------------------------------------------------------------- + +void * FlowSpaceConsensusWorker(void *input) { + ConsensusContext& vc = *static_cast(input); + + vector::iterator indel_target = vc.targets_manager->merged.begin(); + vector::iterator depth_target = vc.targets_manager->merged.begin(); + + const static int kReadBatchSize = 40; + Alignment * new_read[kReadBatchSize]; + bool success[kReadBatchSize]; + PersistingThreadObjects thread_objects(*vc.global_context); + + list ticket_temp_1(1), ticket_temp_2(1); + + // This is the consensus reads to be saved, no realignment needed. + list::iterator consensus_position_ticket = ticket_temp_1.begin(); + consensus_position_ticket->begin = NULL; + consensus_position_ticket->end = NULL; + // This is the consensus reads to be saved, realignment needed + list::iterator aln_needed_consensus_position_ticket = ticket_temp_2.begin(); + aln_needed_consensus_position_ticket->begin = NULL; + aln_needed_consensus_position_ticket->end = NULL; + + list::iterator target_ticket; + + bool more_positions = true; + const bool use_molecular_tag = vc.mol_tag_manager->tag_trimmer->HaveTags(); + const int effective_min_family_size = use_molecular_tag? vc.parameters->tag_trimmer_parameters.min_family_size : 1; + const int max_read_num_in_memory = use_molecular_tag? 250000 : 50000; + + int sample_num = 1; + if (vc.consensus_parameters->multisample){ + sample_num = vc.sample_manager->num_samples_; + } + + vector< vector< vector > > my_molecular_families_multisample; + MolecularFamilyGenerator my_family_generator; + + // Initialize flowspace_consensus_master + string basecaller_ver, tmap_ver; + vc.bam_walker->GetProgramVersions(basecaller_ver, tmap_ver); + FlowSpaceConsensusMaster flowspace_consensus_master(&thread_objects, vc.ref_reader, vc.global_context, basecaller_ver); + + // Propagate parameters + flowspace_consensus_master.PropagateFlowspaceConsensusParameters(*(vc.consensus_parameters), use_molecular_tag); + if (not vc.consensus_parameters->skip_consensus){ + flowspace_consensus_master.InitializeConsensusCounter(); // If not initialized, then I won't see the stat if no family is generated. + } + while (true /*more_positions*/) { + + // Opportunistic read removal + // Note: The I need to make sure that I am not attempt to remove the reads in progress. + pthread_mutex_lock(&vc.bam_walker_mutex); + if (vc.consensus_bam_walker->EligibleForTargetBasedReadRemoval()) { + if (pthread_mutex_trylock(&vc.read_removal_mutex) == 0) { + Alignment *removal_list = NULL; + vc.consensus_bam_walker->RequestTargetBasedReadRemovalTask(removal_list); + pthread_mutex_unlock(&vc.bam_walker_mutex); + //In rare case, the Eligible check pass, but another thread got to remove reads, then when this thread get the lock, it find there + //is no reads to remove. The unexpected behavior of SaveAlignment() is that when NULL is passed in, it save all the reads and remove + // ZM tags. To prevent that, we need to check for empty. + if (removal_list) { + Alignment* save_list = vc.bam_writer->process_new_entries(removal_list); + //vc.bam_walker->SaveAlignments(save_list, vc, depth_target); + pthread_mutex_lock(&vc.bam_walker_mutex); + vc.bam_walker->FinishReadRemovalTask(save_list, max_read_num_in_memory + 5000); + pthread_mutex_unlock(&vc.bam_walker_mutex); + } + pthread_mutex_unlock(&vc.read_removal_mutex); + pthread_cond_broadcast(&vc.memory_contention_cond); + }else{ + pthread_mutex_unlock(&vc.bam_walker_mutex); + } + } + else{ + pthread_mutex_unlock(&vc.bam_walker_mutex); + } + + // If too many reads in memory and at least one candidate evaluator in progress, pause this thread + // Wake up when the oldest candidate evaluator task is completed. + + pthread_mutex_lock(&vc.bam_walker_mutex); + if (vc.bam_walker->MemoryContention(max_read_num_in_memory)) { + pthread_cond_wait (&vc.memory_contention_cond, &vc.bam_walker_mutex); + pthread_mutex_unlock(&vc.bam_walker_mutex); + continue; + } + pthread_mutex_unlock(&vc.bam_walker_mutex); + + + // + // Task dispatch: Decide whether to load more reads or to generate more variants + // + bool ready_for_next_position = false; + + if (vc.bam_walker->EligibleForGreedyRead()) { + // Greedy reading allowed: if candidate generation in progress, just grab a new read + if (true/*pthread_mutex_trylock(&vc.candidate_generation_mutex) == 0*/) { + pthread_mutex_lock(&vc.bam_walker_mutex); + ready_for_next_position = vc.bam_walker->ReadyForNextPosition(); + if (not ready_for_next_position) { + pthread_mutex_unlock(&vc.bam_walker_mutex); +// pthread_mutex_unlock(&vc.candidate_generation_mutex); + } + } + + } else { + // Greedy reading disallowed: if candidate generation in progress, + // wait for it to finish before deciding what to do. +// pthread_mutex_lock(&vc.candidate_generation_mutex); + pthread_mutex_lock(&vc.bam_walker_mutex); + ready_for_next_position = vc.bam_walker->ReadyForNextPosition(); + if (not ready_for_next_position) { + pthread_mutex_unlock(&vc.bam_walker_mutex); +// pthread_mutex_unlock(&vc.candidate_generation_mutex); + } + } + + + + // + // Dispatch outcome: Load and process more reads + // + + if (not ready_for_next_position) { + + + pthread_mutex_lock(&vc.read_loading_mutex); + if (not vc.bam_walker->HasMoreAlignments()) { + pthread_mutex_unlock(&vc.read_loading_mutex); + pthread_cond_broadcast(&vc.memory_contention_cond); + break; + } + + pthread_mutex_lock(&vc.bam_walker_mutex); + for (int i = 0; i < kReadBatchSize; ++i) { + vc.bam_walker->RequestReadProcessingTask(new_read[i]); + success[i] = false; + } + pthread_mutex_unlock(&vc.bam_walker_mutex); + + for (int i = 0; i < kReadBatchSize; ++i) { + success[i] = vc.bam_walker->GetNextAlignmentCore(new_read[i], vc, indel_target); + if (not success[i]) + { break; } + } + pthread_mutex_unlock(&vc.read_loading_mutex); + + + for (int i = 0; i < kReadBatchSize and success[i]; ++i) { + // 1) Filling in read body information and do initial set of read filters + if (not vc.candidate_generator->BasicFilters(*new_read[i])) + { continue; } + + // 2) Alignment information altering methods (can also filter a read) + if (not vc.mol_tag_manager->tag_trimmer->GetTagsFromBamAlignment(new_read[i]->alignment, new_read[i]->tag_info)) { + new_read[i]->filtered = true; + continue; + } + + // Filter by read mismatch limit (note: NOT the filter for read-max-mismatch-fraction) here. + // Note: Target override did not apply for consensus. + FilterByModifiedMismatches(new_read[i], vc.consensus_parameters->read_mismatch_limit, NULL); + if (new_read[i]->filtered) + continue; + + // 3) Filter by target + // These two variables should be parameters of consensus. + vc.targets_manager->FilterReadByRegion(new_read[i], vc.bam_walker->GetRecentUnmergedTarget()); + if (new_read[i]->filtered) + { continue; } + // 4) Unpacking read meta data for flow-space consensus + UnpackOnLoadLight(new_read[i], *vc.global_context); + // 5) Count how many reads that use the program for alignment. + vc.consensus_bam_walker->AddReadToPG(new_read[i]); + } + + pthread_mutex_lock(&vc.bam_walker_mutex); + for (int i = 0; i < kReadBatchSize; ++i) { + vc.bam_walker->FinishReadProcessingTask(new_read[i], success[i]); + } + pthread_mutex_unlock(&vc.bam_walker_mutex); + + continue; + } + + // + // Dispatch outcome: Generate candidates at next position + // + + if (not vc.bam_walker->HasMoreAlignments() and not more_positions) { + pthread_mutex_unlock(&vc.bam_walker_mutex); +// pthread_mutex_unlock(&vc.candidate_generation_mutex); + pthread_cond_broadcast(&vc.memory_contention_cond); + break; + } + + pthread_mutex_unlock(&vc.bam_walker_mutex); + pthread_mutex_lock(&vc.bam_walker_mutex); + + // I do consensus only if I travel to the position at the end of the target. + bool do_consensus = vc.bam_walker->getPosition() == (vc.bam_walker->getEndPosition() - 1); + + if (not do_consensus){ + more_positions = vc.bam_walker->AdvancePosition(1); + pthread_mutex_unlock(&vc.bam_walker_mutex); + continue; + } + + // Generating the target ticket that consists of the reads that cover the target. + vc.consensus_bam_walker->BeginTargetProcessingTask(target_ticket); + + // The target_ticket has been generated. Now I can move to the next position (in fact, next target) to let other threads keep working. + // But keep in mind, don't let other thread remove the reads that I am processing. + more_positions = vc.bam_walker->AdvancePosition(1); + pthread_mutex_unlock(&vc.bam_walker_mutex); + + // Now I am going to gather the reads to form consensus reads. + my_molecular_families_multisample.resize(sample_num); + + // I need to make sure that every read won't be processed twice. + // i.e., no read can appear in two or more consensus reads. + // So I use read_filter_mutex to prevent any other thread is using the same read to generate another consensus read. + pthread_mutex_lock(&vc.read_filter_mutex); + // Generate families if I am using molecular tags. + if (use_molecular_tag){ + for (int sample_index = 0; sample_index < sample_num; ++sample_index) { + int overloaded_sample_index = vc.consensus_parameters->multisample ? sample_index : -1; + my_family_generator.GenerateMyMolecularFamilies(vc.mol_tag_manager, *target_ticket, overloaded_sample_index, my_molecular_families_multisample[sample_index]); + // Label all the reads being processed here as filtered, so they won't be use again. + for (vector< vector< MolecularFamily> >::iterator strand_it = my_molecular_families_multisample[sample_index].begin(); strand_it != my_molecular_families_multisample[sample_index].end(); ++strand_it) { + for (vector< MolecularFamily>::iterator fam_it = strand_it->begin(); fam_it != strand_it->end(); ++fam_it) { + for (vector< Alignment *>::iterator read_it = (fam_it->all_family_members).begin(); read_it != (fam_it->all_family_members).end(); ++read_it) { + (*read_it)->filtered = true; + } + } + } + } + } + // Generate "families" if I am NOT using molecular tags. + else{ + for (int sample_index = 0; sample_index < sample_num; ++sample_index) { + // If not using molecular tag, all the reads on the strand form a family. + // i.e., I will create 2 huge families, families of reads on the FWD/REV strands, respectively. + my_molecular_families_multisample[sample_index].resize(2); + for (int strand = 0; strand < 2; ++strand){ + if (my_molecular_families_multisample[sample_index][strand].empty()){ + string barcode_name = (strand == 0) ? "All_FWD_READS" : "All_REV_READS"; + my_molecular_families_multisample[sample_index][strand].push_back(MolecularFamily(barcode_name, strand)); + my_molecular_families_multisample[sample_index][strand][0].all_family_members.reserve(4096); + } + // I reuse all_family_members. + my_molecular_families_multisample[sample_index][strand][0].all_family_members.resize(0); + } + } + for (Alignment* rai = target_ticket->begin; rai != target_ticket->end; rai = rai->next) { + if (rai == NULL) { + target_ticket->end = NULL; + break; + } + if (rai->filtered) { + continue; + } + int strand_key = (rai->is_reverse_strand)? 1 : 0; + my_molecular_families_multisample[rai->sample_index][strand_key][0].AddNewMember(rai); + // Label all the reads being processed here as filtered, so they won't be use again. + rai->filtered = true; + } + } + pthread_mutex_unlock(&vc.read_filter_mutex); + + // Now I can generate consensus reads from the families. + GenerateFlowSpaceConsensusPositionTicket(my_molecular_families_multisample, + flowspace_consensus_master, + (unsigned int) effective_min_family_size, + consensus_position_ticket, + aln_needed_consensus_position_ticket, + vc.targets_manager, + vc.consensus_parameters->skip_consensus); + + if (not vc.consensus_parameters->skip_consensus){ + // Save consensus_position_ticket and aln_needed_consensus_position_ticket to the bam files + vc.consensus_bam_walker->SaveConsensusAlignments(consensus_position_ticket->begin, aln_needed_consensus_position_ticket->begin); + + // delete consensus_position_ticket + while ((consensus_position_ticket->begin != NULL) and (consensus_position_ticket->begin != consensus_position_ticket->end)) { + Alignment* p = consensus_position_ticket->begin; + consensus_position_ticket->begin = consensus_position_ticket->begin->next; + delete p; + } + if (consensus_position_ticket->begin != NULL) { + delete consensus_position_ticket->begin; + consensus_position_ticket->begin = NULL; + consensus_position_ticket->end = NULL; + } + // delete aln_needed_consensus_position_ticket + while ((aln_needed_consensus_position_ticket->begin != NULL) and (aln_needed_consensus_position_ticket->begin != aln_needed_consensus_position_ticket->end)) { + Alignment* p = aln_needed_consensus_position_ticket->begin; + aln_needed_consensus_position_ticket->begin = aln_needed_consensus_position_ticket->begin->next; + delete p; + } + if (aln_needed_consensus_position_ticket->begin != NULL) { + delete aln_needed_consensus_position_ticket->begin; + aln_needed_consensus_position_ticket->begin = NULL; + aln_needed_consensus_position_ticket->end = NULL; + } + } +// pthread_mutex_unlock(&vc.candidate_generation_mutex); + pthread_mutex_lock(&vc.bam_walker_mutex); + bool signal_contention_removal = vc.bam_walker->IsEarlierstPositionProcessingTask(target_ticket); + vc.bam_walker->FinishPositionProcessingTask(target_ticket); + pthread_mutex_unlock(&vc.bam_walker_mutex); + + if (signal_contention_removal) + { pthread_cond_broadcast(&vc.memory_contention_cond); } + + if (time(NULL) > vc.dot_time) { + cerr << '.'; + vc.dot_time += 30; + } + } + return NULL; +} + diff --git a/Analysis/VariantCaller/Consensus/Consensus.h b/Analysis/VariantCaller/Consensus/Consensus.h new file mode 100644 index 00000000..633e9dfd --- /dev/null +++ b/Analysis/VariantCaller/Consensus/Consensus.h @@ -0,0 +1,13 @@ +/* + * Consensus.h + * + * Created on: Feb 9, 2017 + * Author: baic1 + */ + +#ifndef CONSENSUS_H +#define CONSENSUS_H + +int ConsensusMain(int argc, char* argv[]); + +#endif // CONSENSUS_H // diff --git a/Analysis/VariantCaller/Consensus/ConsensusParameters.cpp b/Analysis/VariantCaller/Consensus/ConsensusParameters.cpp new file mode 100644 index 00000000..fc3bd70e --- /dev/null +++ b/Analysis/VariantCaller/Consensus/ConsensusParameters.cpp @@ -0,0 +1,213 @@ +/* Copyright (C) 2016 Thermo Fisher Scientific, Inc. All Rights Reserved */ + +#include "ConsensusParameters.h" +#include "MolecularTagTrimmer.h" +#include "MiscUtil.h" +#include +#include +#include +#include +#include +#include +#include +#include + +using namespace std; + + +void ConsensusHelp() { + printf("Usage: consensus [options]\n"); + printf("\n"); + + printf("General options:\n"); + printf(" -h,--help print this help message and exit\n"); + printf(" -v,--version print version and exit\n"); + printf(" -n,--num-threads INT number of worker threads [4]\n"); + printf(" --parameters-file FILE json file with algorithm control parameters [optional]\n"); + printf("\n"); + + printf("Inputs:\n"); + printf(" -r,--reference FILE reference fasta file [required]\n"); + printf(" -b,--input-bam FILE bam file with mapped reads [required]\n"); + printf(" -t,--target-file FILE only process targets in this bed file [required]\n"); + printf("\n"); + + printf("Outputs:\n"); + printf(" -O,--output-dir DIRECTORY base directory for all output files [current dir]\n"); + printf(" --consensus-bam FILE save processed consensus reads to the BAM file [required]\n"); + + printf("\n"); + + MolecularTagTrimmer::PrintHelp(true); + printf("\n"); + + printf("BaseCaller options:\n"); + printf(" --suppress-recalibration on/off suppress homopolymer recalibration [on].\n"); + printf(" --use-sse-basecaller on/off switch to use the vectorized version of the basecaller [on].\n"); + printf("\n"); + + + printf("Read filtering options:\n"); + printf(" -M,--min-mapping-qv INT do not use reads with mapping quality below this [4]\n"); + printf(" -U,--read-snp-limit INT do not use reads with number of snps above this [10]\n"); + printf(" --read-mismatch-limit INT do not use reads with number of mismatches (where 1 gap open counts 1) above this value (0 to disable this option) [0]\n"); + printf(" --min-cov-fraction FLOAT do not use reads with fraction of covering the best assigned (unmerged) target region below this [0.0]\n"); + printf("\n"); + + printf("Consensus read output options:\n"); + printf(" --need-3-end-adapter on/off do not output consensus reads w/o 3\" adapter found [off]\n"); + printf(" --filter-qt-reads on/off do not output quality-trimmed consensus reads [off]\n"); + printf(" --filter-single-read-consensus on/off do not output single-read consensus [off]\n"); + printf("\n"); + + printf("Debug:\n"); + printf(" --skip-consensus on/off skip all calculations for consensus; output targets_depth.txt only [off]\n"); + printf("\n"); + +} + +ConsensusParameters::ConsensusParameters(int argc, char** argv) +{ + // i/o parameters: + fasta = ""; // -f --fasta-reference + targets = ""; // -t --targets + outputDir = ""; + consensus_bam = ""; + + + //OptArgs opts; + opts.ParseCmdLine(argc, (const char**)argv); + + if (argc == 1) { + ConsensusHelp(); + exit(0); + } + if (opts.GetFirstBoolean('v', "version", false)) { + exit(0); + } + if (opts.GetFirstBoolean('h', "help", false)) { + ConsensusHelp(); + exit(0); + } + + // enable floating point exceptions during program execution + if (opts.GetFirstBoolean('-', "float-exceptions", true)) { + cout << "consensus: Floating point exceptions enabled." << endl; + feraiseexcept(FE_DIVBYZERO | FE_INVALID | FE_OVERFLOW); + } + + Json::Value tvc_params(Json::objectValue); + Json::Value freebayes_params(Json::objectValue); + Json::Value params_meta(Json::objectValue); + ParametersFromJSON(opts, tvc_params, freebayes_params, params_meta); + + prefixExclusion = opts.GetFirstInt('-', "prefix-exclude", 6); + cerr << "prefix-exclude = " << prefixExclusion << endl; + + params_meta_name = params_meta.get("name",string()).asString(); + params_meta_details = params_meta.get("configuration_name", string()).asString(); + string external_file = params_meta.get("external_file", string()).asString(); + if (not external_file.empty()) { + if (not params_meta_details.empty()) {params_meta_details += ", ";} + params_meta_details += external_file; + } + string repository_id = params_meta.get("repository_id",string()).asString(); + string ts_version = params_meta.get("ts_version","").asString(); + if (not repository_id.empty()) { + if (not params_meta_details.empty()) + params_meta_details += ", "; + params_meta_details += repository_id; + } + if (not ts_version.empty()) { + if (not params_meta_details.empty()) + params_meta_details += ", "; + params_meta_details += "TS version: "; + params_meta_details += ts_version; + } + + // Retrieve the parameters that consensus needed + program_flow.nThreads = RetrieveParameterInt (opts, tvc_params, 'n', "num-threads", 4); + program_flow.suppress_recalibration = RetrieveParameterBool (opts, tvc_params, '-', "suppress-recalibration", true); +#ifdef __SSE3__ + program_flow.use_SSE_basecaller = RetrieveParameterBool (opts, tvc_params, '-', "use-sse-basecaller", true); +#else + program_flow.use_SSE_basecaller = RetrieveParameterBool (opts, tvc_params, '-', "use-sse-basecaller", false); +#endif + min_mapping_qv = RetrieveParameterInt (opts, freebayes_params, 'M', "min-mapping-qv", 4); + min_cov_fraction = RetrieveParameterDouble(opts, freebayes_params, '-', "min-cov-fraction", 0.0f); + read_mismatch_limit = RetrieveParameterInt (opts, freebayes_params, '-', "read-mismatch-limit", 0); + read_snp_limit = RetrieveParameterInt (opts, freebayes_params, 'U', "read-snp-limit", 10); + need_3_end_adapter = opts.GetFirstBoolean('-', "need-3-end-adapter", false); + filter_qt_reads = opts.GetFirstBoolean('-', "filter-qt-reads", false); + filter_single_read_consensus = opts.GetFirstBoolean('-', "filter-single-read-consensus", false); + skip_consensus = opts.GetFirstBoolean('-', "skip-consensus", false); + + SetMolecularTagTrimmerOpt(tvc_params); + + cout << endl; + + // Check limit + CheckParameterLowerBound ("num-threads", program_flow.nThreads, 1); + CheckParameterLowerUpperBound("min-cov-fraction", min_cov_fraction, 0.0f, 1.0f); + CheckParameterLowerBound ("read-snp-limit", read_snp_limit, 0); + CheckParameterLowerBound ("min-mapping-qv", min_mapping_qv, 0); + CheckParameterLowerBound ("min-mapping-qv", min_mapping_qv, 0); + CheckParameterLowerBound ("read-mismatch-limit", read_mismatch_limit, 0); + CheckParameterLowerUpperBound ("tag-trim-method", tag_trimmer_parameters.tag_trim_method, 0, 2); + CheckParameterLowerBound ("min-tag-fam-size", tag_trimmer_parameters.min_family_size, 1); + cout << endl; + + SetupFileIO(opts); +} + +void ConsensusParameters::SetupFileIO(OptArgs &opts) +{ + // Reference fasta + fasta = opts.GetFirstString('r', "reference", ""); + if (fasta.empty()) { + cerr << "FATAL ERROR: Reference file not specified via -r" << endl; + exit(-1); + } + ValidateAndCanonicalizePath(fasta); + + // Region bed + targets = opts.GetFirstString('t', "target-file", ""); + if (targets.empty()) { + cerr << "FATAL ERROR: Target file not specified!" << endl; + exit(-1); + } + ValidateAndCanonicalizePath(targets); + + // Input bam + opts.GetOption(bams, "", 'b', "input-bam"); + if (bams.empty()) { + cerr << "FATAL ERROR: BAM file not specified via -b" << endl; + exit(-1); + } + for (unsigned int i_bam = 0; i_bam < bams.size(); ++i_bam) + ValidateAndCanonicalizePath(bams[i_bam]); + + // Output dir + outputDir = opts.GetFirstString('O', "output-dir", "."); + mkpath(outputDir, S_IRWXU | S_IRWXG | S_IROTH | S_IXOTH); // XXX nope! + ValidateAndCanonicalizePath(outputDir); + + // output consensus bam + string dir = ""; + if (skip_consensus){ + consensus_bam = ""; + dir = outputDir + "/"; + }else{ + consensus_bam = opts.GetFirstString('-', "consensus-bam", ""); + consensus_bam = outputDir + "/" + consensus_bam; + dir = consensus_bam.substr(0, consensus_bam.find_last_of("/\\")); + } + mkpath(dir, S_IRWXU | S_IRWXG | S_IROTH | S_IXOTH); + ValidateAndCanonicalizePath(dir); + + // Options for multisample. Currently not supported. + /* + sampleName = opts.GetFirstString('g', "sample-name", ""); + force_sample_name = opts.GetFirstString('-', "force-sample-name", ""); + */ +} diff --git a/Analysis/VariantCaller/Consensus/ConsensusParameters.h b/Analysis/VariantCaller/Consensus/ConsensusParameters.h new file mode 100644 index 00000000..b0a2225a --- /dev/null +++ b/Analysis/VariantCaller/Consensus/ConsensusParameters.h @@ -0,0 +1,32 @@ +/* Copyright (C) 2016 Thermo Fisher Scientific, Inc. All Rights Reserved */ + +#ifndef CONSENSUSPARAMETERS_H +#define CONSENSUSPARAMETERS_H + +#include +#include +#include "OptArgs.h" +#include "OptBase.h" +#include "json/json.h" +#include "ExtendParameters.h" + +using namespace std; + + +class ConsensusParameters : public ExtendParameters { +public: + string consensus_bam; // Base name of the two consensus bam files. + + bool need_3_end_adapter = false; + bool filter_qt_reads = false; + bool filter_single_read_consensus = false; + bool skip_consensus = false; +// bool use_flowspace_clustering; // generate one consensus read for each flowspace cluster + // functions + ConsensusParameters(int argc, char** argv); + void SetupFileIO(OptArgs &opts); +}; + + +#endif // CONSENSUSPARAMETERS_H + diff --git a/Analysis/VariantCaller/Consensus/FlowSpaceConsensus.cpp b/Analysis/VariantCaller/Consensus/FlowSpaceConsensus.cpp new file mode 100644 index 00000000..b0136951 --- /dev/null +++ b/Analysis/VariantCaller/Consensus/FlowSpaceConsensus.cpp @@ -0,0 +1,1165 @@ +/* Copyright (C) 2016 Thermo Fisher Scientific, Inc. All Rights Reserved */ + +//! @file FlowSpaceConsensus.cpp +//! @ingroup VariantCaller +//! @brief Cluster flow-synchronized reads; generate consensus measurements. + +#include "FlowSpaceConsensus.h" + +#include +#include "MiscUtil.h" +#include "ExtendedReadInfo.h" + +class ConsensusCounter { +private: + pthread_mutex_t mutextConsensusCounter_; + unsigned int num_func_fam_ = 0; + unsigned int num_consensus_reads_ = 0; + unsigned int num_single_read_consensus_ = 0; + unsigned int num_reads_in_func_fam_ = 0; + unsigned int num_reads_in_consensus_bam_ = 0; + unsigned int num_consensus_reads_need_aln_ = 0; + void PrintSummary_() + { + if (use_molecular_tag){ + cout << "[Stat] Summary of the flow-space consensus:" << endl + << " - Number of functional families identified = " << num_func_fam_ << endl + << " - Number of reads in the functional families identified = " << num_reads_in_func_fam_ << endl + << " - Number of consensus reads generated = " << num_consensus_reads_ << endl + << " - Number of consensus reads realigned by tmap = " << num_consensus_reads_need_aln_ << endl + << " - Number of reads that consists of the consensus reads = " << num_reads_in_consensus_bam_ << endl + << " - Number of single-read consensus = " << num_single_read_consensus_ << endl; + cout << " - Compression rate = "<< num_consensus_reads_ <<"/"<< num_reads_in_consensus_bam_ <<" = "; + if (num_reads_in_consensus_bam_ == 0){ + cout << "NaN"<< endl; + }else{ + cout << (double) num_consensus_reads_ / (double) num_reads_in_consensus_bam_ << endl; + } + cout << " - Average number of consensus reads per functional family = "; + if (num_func_fam_ == 0){ + cout << "NaN" << endl << endl << endl; + }else{ + cout << (double) num_consensus_reads_ / (double) num_func_fam_ << endl << endl << endl; + } + } + else{ + cout << "[Stat] Summary of the flow-space consensus:" << endl + << " - Number of reads processed = " << num_reads_in_func_fam_ << endl + << " - Number of reads that consists of the consensus reads = " << num_reads_in_consensus_bam_ << endl + << " - Number of consensus reads generated = " << num_consensus_reads_ << endl + << " - Number of consensus reads realigned by tmap = " << num_consensus_reads_need_aln_ << endl + << " - Number of single-read consensus = " << num_single_read_consensus_ << endl + << " - Compression rate = "<< num_consensus_reads_ <<"/"<< num_reads_in_consensus_bam_ << " = "; + if (num_reads_in_consensus_bam_ == 0){ + cout << "NaN" << endl << endl << endl; + }else{ + cout << (double) num_consensus_reads_ / (double) num_reads_in_consensus_bam_ << endl << endl << endl; + } + } + } +public: + ConsensusCounter(){}; + ConsensusCounter(bool is_mol_tag){ use_molecular_tag = is_mol_tag; }; + bool use_molecular_tag = false; + virtual ~ConsensusCounter() {PrintSummary_();} + void Count(unsigned int num_func_fam, + unsigned int num_consensus_reads, + unsigned int num_single_read_consensus, + unsigned int num_reads_in_func_fam, + unsigned int num_reads_in_consensus_bam, + unsigned int num_consensus_reads_need_aln) + { + pthread_mutex_lock(&mutextConsensusCounter_); + num_func_fam_ += num_func_fam; + num_consensus_reads_ += num_consensus_reads; + num_single_read_consensus_ += num_single_read_consensus; + num_reads_in_func_fam_ += num_reads_in_func_fam; + num_reads_in_consensus_bam_ += num_reads_in_consensus_bam; + num_consensus_reads_need_aln_ += num_consensus_reads_need_aln; + pthread_mutex_unlock(&mutextConsensusCounter_); + } +}; + +// Inputs: base_seq (can be vector or string), flow_order (any type with operator [] defined), num_flows (num_flows is the length of flow_order) +// Outputs: flowgram, base_idx_to_flow_idx +// Convert base_seq to flowgram associated with the flow_order +// base_idx_to_flow_idx[base_idx] is the flow index where base_seq[base_idx] incorporates. +// base_idx_to_flow_idx[base_idx] is set to -1 if incorporated flow of base_seq[base_idx] can not be specified by flow_order. +template +void BaseSeqToFlowgram (const BaseSeqI &base_seq, const FlowOrderI &flow_order, int num_flows, vector &flowgram, vector &base_idx_to_flow_idx) +{ + int flow_idx = 0; + unsigned int num_bases = base_seq.size(); + unsigned int base_idx = 0; + + base_idx_to_flow_idx.assign(base_seq.size(), -1); + flowgram.assign(num_flows, 0); + + while (flow_idx < num_flows and base_idx < num_bases) { + while (base_idx < num_bases and flow_order[flow_idx] == base_seq.at(base_idx)) { + base_idx_to_flow_idx[base_idx] = flow_idx; + ++flowgram[flow_idx]; + ++base_idx; + } + ++flow_idx; + } +} + +// This function is used to fix the bug in the ZC tag where it is possible to get ZC[0] != ZC[1] while flow_order[ZC[0]] = flow_order[ZC[1]] +// The bug is fixed in 5.2.1. +bool FixZcBug(vector &zc, const ion::FlowOrder& flow_order) +{ + bool is_fixed = false; + if (zc[0] != zc[1] and flow_order[zc[0]] == flow_order[zc[1]]){ + if (zc[0] < zc[1]) + zc[1] = zc[0]; + else + zc[0] = zc[1]; + is_fixed = true; + } + return is_fixed; +} + +// A "softer" criterion to determine x_mer == y_mer that allows some hp error. +// I claim x_mer and y_mer are "very different" if max(x_mer, y_mer) - min(x_mer, y_mer) > kHpLenDiffAllowed[max(x_mer, y_mer)] +// return true if x_mer and y_mer are "very different". +// Here kHpLenDiffAllowed is set heuristically. +//@TODO: optimize kHpLenDiffAllowed or come up with a better algorithm (e.g. K-run). +bool SofterHpDisriminator(int x_mer, int y_mer) +{ + const static vector kHpLenDiffAllowed = {0, 0, 1, 1, 2, 2, 3, 3, 3, 4, 4, 4, 5, 5, 5, 5, 5, 6, 6, 6, 6, 7, 7, 7, 8}; // I hard code the criterion. + const static int kMaxHpDefined = (int) kHpLenDiffAllowed.size() - 1; + return (abs(x_mer - y_mer) > kHpLenDiffAllowed[min(max(x_mer, y_mer), kMaxHpDefined)]); +} + +// base_seq = (KS + ZT + ZE) + template + (YE + YT) +// Here I do not reconstruct the 3' adapter because I don't have enough information. +void ReconstructBaseAsCalled(const Alignment* rai, string &base_seq) +{ + string read_base = rai->alignment.QueryBases; + if (rai->is_reverse_strand){ + RevComplementInPlace(read_base); + } + base_seq = rai->prefix_bases + read_base + rai->suffix_bases; +} + +// return the first/last hp length of base_seq if is_reverse_mode = true/false, respectively. +int FindFirstHpLen(const string& base_seq, bool is_reverse_mode = false) +{ + int base_seq_size = (int) base_seq.size(); + if (base_seq_size <= 1) + return base_seq_size; + int first_hp_len = 1; + int current_idx = 1; + int increment = 1; + char first_nuc = base_seq.at(0); + if (is_reverse_mode){ + current_idx = base_seq_size - 2; + increment = -1; + first_nuc = base_seq.at(base_seq_size - 1); + } + while (current_idx >= 0 and current_idx < base_seq_size){ + if (base_seq.at(current_idx) == first_nuc) + ++first_hp_len; + else + return first_hp_len; + current_idx += increment; + } + return first_hp_len; +} + +// Find the smallest index i >= start_flow such that flowgram_0[i] = flowgram_1[i] > 0 +// return -1 if not found +int GetNextIncorpFlowInCommon(const vector& flowgram_0, const vector& flowgram_1, unsigned int start_flow) +{ + unsigned int min_len = min(flowgram_0.size(), flowgram_1.size()); + int next_incorp_flow_in_common = -1; + for (unsigned int flow_idx = start_flow; flow_idx < min_len; ++flow_idx){ + if (flowgram_0[flow_idx] == flowgram_1[flow_idx] and flowgram_0[flow_idx] > 0){ + next_incorp_flow_in_common = flow_idx; + return next_incorp_flow_in_common; + } + } + return next_incorp_flow_in_common; +} + +// Find the largest index i <= start_flow such that flowgram_0[i] = flowgram_1[i] > 0 +// return -1 if not found +int GetLastIncorpFlowInCommon(const vector& flowgram_0, const vector& flowgram_1, unsigned int start_flow) +{ + int last_incorp_flow_in_common = -1; + unsigned int min_len = min(flowgram_0.size(), flowgram_1.size()); + if (min_len == 0){ + return last_incorp_flow_in_common; + } + if (start_flow >= min_len){ + start_flow = min_len - 1; + } + for (unsigned int flow_idx = start_flow; flow_idx != 0; --flow_idx){ + if (flowgram_0[flow_idx] == flowgram_1[flow_idx] and flowgram_0[flow_idx] > 0){ + last_incorp_flow_in_common = flow_idx; + return last_incorp_flow_in_common; + } + } + return last_incorp_flow_in_common; +} + +// Find the largest index i <= start_flow such that flowgram_0[i] = flowgram_1[i] > 0 +// return -1 if not found +int GetLastIncorpFlowAlmostInCommon(const vector& flowgram_0, const vector& flowgram_1, unsigned int start_flow) +{ + int last_incorp_flow_in_common = -1; + unsigned int min_len = min(flowgram_0.size(), flowgram_1.size()); + if (min_len == 0){ + return last_incorp_flow_in_common; + } + if (start_flow >= min_len){ + start_flow = min_len - 1; + } + for (unsigned int flow_idx = start_flow; flow_idx != 0; --flow_idx){ + if ((not SofterHpDisriminator(flowgram_0[flow_idx], flowgram_1[flow_idx])) and flowgram_0[flow_idx] > 0 and flowgram_1[flow_idx] > 0){ + last_incorp_flow_in_common = flow_idx; + return last_incorp_flow_in_common; + } + } + return last_incorp_flow_in_common; +} + +// This function determine whether flowgram_0[0:synchronized_len] and flowgram_1[0:synchronized_len] are flow-synchronized or not by simply looking at the discrepancy between the two flowgrams, +// where synchronized_len - 1 is the is last incorporated flow in common (i.e., the largest i such that flowgram_0[i] = flowgram_1[i] > 0). +// Alignment information is not needed! +// I claim the two flowsgrams are flow-synchronized if the following conditions are all satisfied. +// a) There exists no flow s.t. one flowgram is 0-mer while the other one is 2-mer or above. (i.e., 0-mer -> (>1-mer) is claimed to be not a sequencing error.) +// b) number of flows with "discrepancy" <= max_num_diff_flows, where discrepancy is defined by SofterHpDisriminator(...) +// c) number of flows with "discrepancy" in every window <= max_num_local_diff_flows +// where a window is a closed interval [window_start, window_end] where window_start, window_end are the only two incorporated flows in common in the window. +// If strict_flow_sync = true, then I don't allow any (0-mer) <-> (>0-mer) change. +// Consider y = H*x where y, x are the vectors of prediction and flowgram, respectively, H is the phasing matrix. +// strict_flow_sync guarantees the same structure of the phasing matrix. +bool IsFlowSynchronized(const vector& flowgram_0, const vector& flowgram_1, unsigned int& synchronized_len, unsigned int max_num_diff_flows, unsigned int max_num_local_diff_flows, bool strict_flow_sync) +{ + bool is_flow_synchronized = false; + // Get the last incorporated flow where the two flowgrams are in common. + synchronized_len = GetLastIncorpFlowAlmostInCommon(flowgram_0, flowgram_1, flowgram_0.size() - 1) + 1; + + if (synchronized_len == 0){ + return is_flow_synchronized; + } + + vector diff_flow_index; + int max_0_mer_diff = strict_flow_sync? 0 : 1; + diff_flow_index.reserve(max_num_diff_flows); + // Find the diff_flow_index from the back since we can return the function faster if they are not flow-synchronized. + for (unsigned int flow_idx = synchronized_len - 1; flow_idx != 0; --flow_idx){ + // Is there a delta at the flow? + bool is_diff = false; + if (flowgram_0[flow_idx] != flowgram_1[flow_idx]){ + if (flowgram_0[flow_idx] == 0 or flowgram_1[flow_idx] == 0){ + // A non-hp indel delta found. + // I don't allow 0-mer <-> (>1-mer). + // I don't allow 0-mer <-> (>0-mer) if strict_flow_sync = true. + // Claim not flow-synchronized. + if (abs((int) flowgram_0[flow_idx] - (int) flowgram_1[flow_idx]) > max_0_mer_diff){ + synchronized_len = 0; + return is_flow_synchronized; + } + is_diff = true; + } + // hp-indel + else if (SofterHpDisriminator((int) flowgram_0[flow_idx], (int) flowgram_1[flow_idx])){ + // The HP lengths of the two flowgram seem very different. + is_diff = true; + } + + if (is_diff){ + // The two flowgrams seem very different at the flow. + diff_flow_index.push_back(flow_idx); + if (diff_flow_index.size() > max_num_diff_flows){ + // The flows with discrepancy is greater than max_num_diff_flows. + // Claim not flow-synchronized. + synchronized_len = 0; + return is_flow_synchronized; + } + } + } + } + + if (diff_flow_index.size() <= max_num_local_diff_flows){ + // num_local_diff won't be greater than max_num_local_diff_flows + // I claim the two flowgrams are flow-synchronized. + is_flow_synchronized = true; + return is_flow_synchronized; + } + + // Define window = closed interval of [window_start, window_end] + int window_end = GetNextIncorpFlowInCommon(flowgram_0, flowgram_1, diff_flow_index[0]); + int window_start = window_end; + int i_diff = 0; + + // num_local_diff = the number of flows with discrepancy in the window + // I claim the flowgrams are not flow-synchronized if num_local_diff > max_num_local_diff_flows in any window. + // Sliding window approach to check num_local_diff for every window with complexity = Big O(diff_flow_index.size()) + // Note that diff_flow_index "must" be in "decreasing" order! + while (i_diff < (int) diff_flow_index.size() and window_start > -1){ + if ((int) diff_flow_index[i_diff] < window_start){ + window_end = window_start; + window_start = GetLastIncorpFlowInCommon(flowgram_0, flowgram_1, diff_flow_index[i_diff]); + unsigned int num_local_diff = 0; + int j_diff = i_diff; + while ((int) diff_flow_index[j_diff] >= window_start and j_diff < (int) diff_flow_index.size()){ + ++num_local_diff; + if (num_local_diff > max_num_local_diff_flows){ + // I find one window with num_local_diff > max_num_local_diff_flows + synchronized_len = 0; + return is_flow_synchronized; + } + ++j_diff; + } + i_diff = j_diff; + } + } + + // Now I have num_local_diff <= max_num_local_diff_flows in all windows. + // Claim the two flowgrams are flow-synchronized. + is_flow_synchronized = true; + return is_flow_synchronized; +} + +PrecomputeForClustering::PrecomputeForClustering(const Alignment* rai){ + if (not rai->alignment.GetTag("ZC", zc)){ + vector u_zc; + if (rai->alignment.GetTag("ZC", u_zc)){ + zc.assign(u_zc.size(), 0); + for (unsigned int i = 0; i < u_zc.size(); ++i){ + zc[i] = (int) u_zc[i]; + } + } + } + if ((not zc.empty()) and zc.size() < 3){ + cerr << "ERROR: the ZC tag with number of fields < 3 in read " << rai->alignment.Name << endl; + exit(1); + } + ReconstructBaseAsCalled(rai, base_seq); +} + +// ============================================================================== + +FlowSpaceCluster::FlowSpaceCluster(const Alignment* new_rai, const ion::FlowOrder* fo) + : flow_order_(fo), runid(new_rai->runid), flow_order_index(new_rai->flow_order_index), is_reverse_strand(new_rai->is_reverse_strand) +{ + PrecomputeForClustering precomp_for_new_rai(new_rai); + InitializeCluster_(new_rai); + AddNewMember_(new_rai, precomp_for_new_rai); +} + + +FlowSpaceCluster::FlowSpaceCluster(const Alignment* new_rai, const vector& flow_order_vector, PrecomputeForClustering& precomp_for_new_rai) + : flow_order_(&(flow_order_vector[new_rai->flow_order_index])), runid(new_rai->runid), flow_order_index(new_rai->flow_order_index), is_reverse_strand(new_rai->is_reverse_strand) +{ + InitializeCluster_(new_rai); + AddNewMember_(new_rai, precomp_for_new_rai); +}; + + +void FlowSpaceCluster::InitializeCluster_(const Alignment* new_rai) +{ + trim_info.prefix_bases = new_rai->prefix_bases; + trim_info.suffix_bases = new_rai->suffix_bases; + trim_info.prefix_end_flow = new_rai->prefix_flow; + trim_info.prefix_end_hp = FindFirstHpLen(trim_info.prefix_bases, true); + trim_info.suffix_start_hp = FindFirstHpLen(trim_info.suffix_bases, false); +} + +void FlowSpaceCluster::AddNewMember_(const Alignment* new_rai, PrecomputeForClustering& precomp_for_new_rai) +{ + cluster_members.push_back(new_rai); + // I use the cluster member with the highest mapping quality to represent this cluster. + if (new_rai->alignment.MapQuality > best_mapping_quality){ + best_mapping_quality = new_rai->alignment.MapQuality; + best_mapping_quality_index = (int) cluster_members.size() - 1; + template_base_seq_ = precomp_for_new_rai.base_seq; + + // Calculate the flowgram if I didn't do it before. + if (precomp_for_new_rai.flowgram.empty() or precomp_for_new_rai.base_index_to_flow_index.empty()){ + BaseSeqToFlowgram(template_base_seq_, *flow_order_, flow_order_->num_flows(), precomp_for_new_rai.flowgram, precomp_for_new_rai.base_index_to_flow_index); + } + template_flowgram_ = precomp_for_new_rai.flowgram; + + // Calculate suffix_start_flow + if (not trim_info.suffix_bases.empty()){ + trim_info.suffix_start_flow = precomp_for_new_rai.base_index_to_flow_index[template_base_seq_.size() - trim_info.suffix_bases.size()]; + } + else{ + // Set to the end flow of template since there is no suffix_bases. + trim_info.suffix_start_flow = precomp_for_new_rai.base_index_to_flow_index.back(); + } + + if (trim_info.suffix_start_flow < 0){ + cerr << "ERROR: insert_base_end_flow outside of [0, num_flows) range in read " << new_rai->alignment.Name << endl; + exit(1); + } + + if (not precomp_for_new_rai.zc.empty()){ + adapter_start_flow = precomp_for_new_rai.zc[0]; + } + else{ + adapter_start_flow = -1; + } + } +} + + +bool FlowSpaceCluster::AskToJoinMe(const Alignment* query_rai) +{ + PrecomputeForClustering precomp_for_query_rai(query_rai); + return AskToJoinMe(query_rai, precomp_for_query_rai); +} + +// The query_rai can join the cluster if query_rai is flow-synchronized with the cluster +// (Note): Sort the reads by the mapping quality in the decreasing order and then call this function is highly recommended! +// (Note): Don't waste your time inputting query_rai if IsIsolateRead(query_rai) is true, since it won't join me anyway. +bool FlowSpaceCluster::AskToJoinMe(const Alignment* query_rai, PrecomputeForClustering& precomp_for_query_rai) +{ + // I only cluster the reads with 3' adapter found for molecular tagging. + // @TODO: It can be an optional constraint for general purpose. + bool is_join = false; + if (precomp_for_query_rai.zc.empty()){ + return is_join; + } + + // The most common reason that a read can't join the cluster. + if (precomp_for_query_rai.zc[0] != adapter_start_flow) // I require the adapter start flow must be the same. + return is_join; + + if (query_rai->runid != runid // The query read must come from the same run + or query_rai->is_reverse_strand != is_reverse_strand // The query read must on the same strand + or query_rai->prefix_bases != trim_info.prefix_bases // The query read must have exactly the same prefix_bases (hp error not allowed) + or query_rai->suffix_bases != trim_info.suffix_bases // The query read must have exactly the same suffix_bases (hp error not allowed) + or query_rai->prefix_flow != trim_info.prefix_end_flow // Let's check the prefix end flow again, though it has been carried out by checking the prefix_bases + or query_rai->flow_order_index != flow_order_index) // I require the same flow order index (it is redundant since every run_id has a unique flow_order index) + return is_join; + + // Easy task: Claim flow-synchronized if base_seq of the query_rai and this->template_base_seq_ are identical. + is_join = precomp_for_query_rai.base_seq == template_base_seq_; + if (is_join){ + AddNewMember_(query_rai, precomp_for_query_rai); + return is_join; + } + + // Calculate the flowgram for query_rai if we haven't done yet. + if (precomp_for_query_rai.flowgram.empty() or precomp_for_query_rai.base_index_to_flow_index.empty()){ + BaseSeqToFlowgram(precomp_for_query_rai.base_seq, *flow_order_, flow_order_->num_flows(), precomp_for_query_rai.flowgram, precomp_for_query_rai.base_index_to_flow_index); + } + + unsigned int synchronized_len = 0; + // Is precomp_for_query_rai.flowgram flow-synchronized with template_flowgram_? + is_join = IsFlowSynchronized(template_flowgram_, precomp_for_query_rai.flowgram, synchronized_len, kMaxNumDiffFlows_, kMaxNumLocalDiffFlows_, kStrictFlowSync_); + if (is_join){ + // Double check if synchronized_len covers suffix_start_flow + // e.g., a quality trimmed read fails this test. + if ((int) synchronized_len < trim_info.suffix_start_flow + 1){ + is_join = false; + } + else{ + AddNewMember_(query_rai, precomp_for_query_rai); + } + return is_join; + } + return is_join; +} + +// ============================================================================== + +FlowSpaceConsensusMaster::FlowSpaceConsensusMaster(PersistingThreadObjects* thread_objects, + const ReferenceReader* reference_reader, + const InputStructures* global_context, + const string& basecaller_ver): + thread_objects_(thread_objects), reference_reader_(reference_reader), global_context_(global_context) +{ + flow_order_index_ = -1; + flow_order_ = NULL; + + is_suffix_counted_in_za_ = false; + // Note that the format of BaseCaller version is "5.2-x" + if (basecaller_ver.substr(0, 4) == "5.2-"){ + long release_ver = strtol(basecaller_ver.substr(4).c_str(), NULL, 0); + // The change of ZA definition starts from BaseCaller 5.2-21. Before that, suffix tags are counted in ZA. + is_suffix_counted_in_za_ = release_ver < 21; + }else if (basecaller_ver.substr(0, 17) == "5.3-0-week160330-"){ + is_suffix_counted_in_za_ = true; + } +} + + +struct RecalGroup{ + vector group_members; + uint16_t mapq_sum; + RecalGroup(){ mapq_sum = 0;}; + RecalGroup(const Alignment* rai){ + group_members = {rai}; + mapq_sum = rai->alignment.MapQuality; + }; + void AddNew(const Alignment* rai){ + group_members.push_back(rai); + mapq_sum += rai->alignment.MapQuality; + }; + // Compare two recalibration groups. + // A group wins if it has more reads. If ties, the group with larger accumulated mapping quality wins. + bool operator>(const RecalGroup& rhs){ + if (group_members.size() == rhs.group_members.size()){ + return (mapq_sum > rhs.mapq_sum); + } + return (group_members.size() > rhs.group_members.size()); + }; +}; + +void FlowSpaceConsensusMaster::InitializeForBaseCalling(const FlowSpaceCluster& my_cluster, const vector& consensus_phase_params, bool suppress_recal, string &consensus_read_name){ + flow_order_index_ = my_cluster.flow_order_index; + flow_order_ = &(global_context_->flow_order_vector.at(flow_order_index_)); + // Set phasing parameters + thread_objects_->SetModelParameters(flow_order_index_, consensus_phase_params); + // Disable use of a previously loaded recalibration model + thread_objects_->DisableRecalibration(flow_order_index_); + + //@TODO: Need to come up with a more clever way to handle recalibration. + bool recalibration_enabled = false; + + // Let's initialize recalibration + if (not suppress_recal and global_context_->do_recal.recal_is_live()){ + recalibration_enabled = true; + vector > recal_groups_vec; + recal_groups_vec.reserve(my_cluster.cluster_members.size()); + for (vector::const_iterator read_it = my_cluster.cluster_members.begin(); read_it != my_cluster.cluster_members.end(); ++read_it){ + string found_key = global_context_->do_recal.FindKey((*read_it)->runid, (*read_it)->well_rowcol[1], (*read_it)->well_rowcol[0]); + MultiAB multi_ab; + global_context_->do_recal.getAB(multi_ab, found_key, (*read_it)->well_rowcol[1], (*read_it)->well_rowcol[0]); + if (not multi_ab.Valid()){ + recalibration_enabled = false; + cerr << "Warning: The read "<< (*read_it)->alignment.Name << " has invalid recalibrartion MultiAB. Recalibration is disabled in the cluster."<< endl; + break; + } + bool group_exists = false; + for (vector >::iterator rc_group_it = recal_groups_vec.begin(); rc_group_it != recal_groups_vec.end(); ++rc_group_it){ + if (multi_ab.aPtr == rc_group_it->first.aPtr and multi_ab.bPtr == rc_group_it->first.bPtr){ + group_exists = true; + rc_group_it->second.AddNew(*read_it); + break; + } + } + if (not group_exists){ + recal_groups_vec.push_back(pair(multi_ab, RecalGroup(*read_it))); + } + } + if (recalibration_enabled){ + vector >::iterator best_rc_group_it = recal_groups_vec.begin(); + for (vector >::iterator rc_group_it = recal_groups_vec.begin(); rc_group_it != recal_groups_vec.end(); ++rc_group_it){ + // Find the "best" recalibration result, what I mean "best" is defined by the > operator + if (rc_group_it->second > best_rc_group_it->second){ + best_rc_group_it = rc_group_it; + } + } + // I use the best recalibration result as the recalibration for the consensus read. + thread_objects_->SetAsBs(flow_order_index_, best_rc_group_it->first.aPtr, best_rc_group_it->first.bPtr); + // query recalibration structure using row, column, entity. + // The consensus_read_name set here will let tvc use the desired recal results. + consensus_read_name = best_rc_group_it->second.group_members[0]->alignment.Name; + } + } + + // Use the read name with the best mapq as the consensus read name if recalibration is not enabled. + if (not recalibration_enabled){ + consensus_read_name = my_cluster.cluster_members[my_cluster.best_mapping_quality_index]->alignment.Name; + } +} + + +// this->InitializeForBaseCalling(short, const vector&) must be done first. +// Input: flow_synchronized_measurements, trim_info +// Outputs: consensus_measurements, prefix_trimmed_consensus_base, measurements_sd +// Call this function if I use void GetMeasurements(const vector&, vector< vector >&) to get flow_synchronized_measurements +bool FlowSpaceConsensusMaster::CalculateConsensusMeasurements(const vector >& flow_synchronized_measurements, + const TrimInfo& trim_info, + vector& consensus_measurements, + vector& measurements_sd) +{ + vector *> flow_synchronized_measurements_ptr; + flow_synchronized_measurements_ptr.assign(flow_synchronized_measurements.size(), NULL); + for (unsigned int i_read = 0; i_read < flow_synchronized_measurements.size(); ++i_read){ + flow_synchronized_measurements_ptr[i_read] = &flow_synchronized_measurements[i_read]; + } + return CalculateConsensusMeasurements(flow_synchronized_measurements_ptr, trim_info, consensus_measurements, measurements_sd); +} + + +// this->InitializeForBaseCalling(short, const vector&) must be done first. +// Input: flow_synchronized_measurements, trim_info +// Outputs: consensus_measurements, prefix_trimmed_consensus_base, measurements_sd +// Call this function if I use void GetMeasurements(const vector&, vector< vector *>&) to get flow_synchronized_measurements +bool FlowSpaceConsensusMaster::CalculateConsensusMeasurements(const vector *>& flow_synchronized_measurements, + const TrimInfo& trim_info, + vector& consensus_measurements, + vector& measurements_sd) +{ + bool success = false; + const unsigned int num_reads_in_cluster = flow_synchronized_measurements.size(); + const double num_reads_in_cluster_double = (double) num_reads_in_cluster; + const double num_reads_in_cluster_minus_one = (double) ((int) num_reads_in_cluster - 1); + + // The flow space consensus is trivial for single read or no read + // I don't attempt to generate consensus measurement for this guy. + if (num_reads_in_cluster <= 1){ + return success; + } + + int min_measurements_len = (int) flow_synchronized_measurements.at(0)->size(); + for (unsigned int i_read = 1; i_read < num_reads_in_cluster; ++i_read){ + min_measurements_len = min(min_measurements_len, (int) flow_synchronized_measurements.at(i_read)->size()); + } + + // consensus_measurements[flow] = mean(flow_synchronized_measurements[:][flow]) + consensus_measurements.resize(flow_order_->num_flows()); // Note that Treephaser requires the length of measurement = length of flow order + // Taking the mean measurements of reads. Set to zero if the flow >= min_measurements_len + for (int i_flow = 0; i_flow < min_measurements_len; ++i_flow){ + double mean_measuremet = 0.0; // Use double to get better numerical precision for cumulative sum + for (unsigned int i_read = 0; i_read < num_reads_in_cluster; ++i_read){ + mean_measuremet += (double) flow_synchronized_measurements.at(i_read)->at(i_flow); + } + mean_measuremet /= num_reads_in_cluster_double; + consensus_measurements.at(i_flow) = (float) mean_measuremet; + } + + // Set data for base calling + consensus_read_.SetData(consensus_measurements, flow_order_->num_flows()); + consensus_read_.sequence.resize(trim_info.prefix_bases.size()); + for (unsigned int i_base = 0; i_base < trim_info.prefix_bases.size(); ++i_base){ + consensus_read_.sequence[i_base] = trim_info.prefix_bases[i_base]; + } + + // Call Treephaser to solve the consensus_measurements + int safe_start_flow = min(trim_info.prefix_end_flow, (int) consensus_read_.normalized_measurements.size() - 1); + thread_objects_->SolveRead(flow_order_index_, consensus_read_, safe_start_flow, min_measurements_len); + + // Check Treephaser got any solution or not + if (consensus_read_.sequence.size() <= trim_info.prefix_bases.size()) + return success; + + // Shrink the length of consensus_measurements to what we want to preserve + consensus_measurements.resize(min_measurements_len); + + // Calculate the standard deviation of the measurements + measurements_sd.resize(min_measurements_len); + for (int i_flow = 0; i_flow < min_measurements_len; ++i_flow){ + double mean_squared_err = 0.0; + for (unsigned int i_read = 0; i_read < num_reads_in_cluster; ++i_read){ + double err = (double) (flow_synchronized_measurements.at(i_read)->at(i_flow) - consensus_measurements.at(i_flow)); + mean_squared_err += (err * err); + } + mean_squared_err /= num_reads_in_cluster_minus_one; // It should be safe since I don't deal with any single read cluster. + measurements_sd.at(i_flow) = (float) sqrt(mean_squared_err); + } + success = true; + return success; +} + +// Apply prefix and suffix trimming on consensus_read_.sequence according to trim_info +// Input: trim_info (contains the trimming information) +// Output: prefix and suffix trimmed base sequence, start_flow +// Note that I do the trimming in flow space -- the bases actually trimmed may be different from trim_info.prefix_base and trim.suffix_bases. +void FlowSpaceConsensusMaster::TrimPrefixSuffixBases(const TrimInfo& trim_info, string& trimmed_consensus_bases, int& start_flow) +{ + vector bases_index_to_flow_index; + vector consensus_flowgram; + BaseSeqToFlowgram(consensus_read_.sequence, *flow_order_, flow_order_->num_flows(), consensus_flowgram, bases_index_to_flow_index); + int trim_prefix_len = 0; + int trim_suffix_len = 0; + + // I shall trim them all if the last incorporated flow is less than trim_info.prefix_end_flow. + if (bases_index_to_flow_index.back() < trim_info.prefix_end_flow){ + trimmed_consensus_bases.resize(0); + start_flow = -1; + return; + } + + for (int flow_idx = 0; flow_idx < trim_info.prefix_end_flow; ++flow_idx){ + trim_prefix_len += consensus_flowgram[flow_idx]; + } + // I suppose to obtain at least (trim_info.prefix_end_hp)-mer at the (trim_info.prefix_end_flow)-th flow, but I don't. + // So I don't trim more than (consensus_flowgram[trim_info.prefix_end_flow])-mer at this flow. + trim_prefix_len += min(consensus_flowgram[trim_info.prefix_end_flow], trim_info.prefix_end_hp); + // start_flow is the first incorporated flow of the template. + start_flow = bases_index_to_flow_index[trim_prefix_len]; + + if (bases_index_to_flow_index.back() >= trim_info.suffix_start_flow){ + for (int flow_idx = bases_index_to_flow_index.back(); flow_idx > trim_info.suffix_start_flow; --flow_idx){ + trim_suffix_len += consensus_flowgram[flow_idx]; + } + // I suppose to obtain at least (trim_info.suffix_start_hp)-mer at the (trim_info.suffix_start_flow)-th flow, but I don't. + // So I don't trim more than (consensus_flowgram[trim_info.suffix_start_flow])-mer at this flow. + trim_suffix_len += min(consensus_flowgram[trim_info.suffix_start_flow], trim_info.suffix_start_hp); + } + + int trimmed_consensus_bases_len = (int) consensus_read_.sequence.size() - (trim_prefix_len + trim_suffix_len); + trimmed_consensus_bases.resize(trimmed_consensus_bases_len); + int untrimmed_base_idx = trim_prefix_len; + for (int trimmed_base_idx = 0; trimmed_base_idx < trimmed_consensus_bases_len; ++trimmed_base_idx){ + trimmed_consensus_bases[trimmed_base_idx] = consensus_read_.sequence[untrimmed_base_idx]; + ++untrimmed_base_idx; + } +} + +// This function works for the case where the measurements have been written in Alignment +void FlowSpaceConsensusMaster::GetMeasurements(const vector& cluster_members, vector *>& flow_synchronized_measurements_ptr) +{ + flow_synchronized_measurements_ptr.assign(cluster_members.size(), NULL); + for (unsigned int read_idx = 0; read_idx < cluster_members.size(); ++read_idx) + { + if (cluster_members[read_idx]->measurements.empty()){ + cerr << "ERROR: Normalized measurements not initialized in read " << cluster_members[read_idx]->alignment.Name << endl; + exit(1); + } + flow_synchronized_measurements_ptr[read_idx] = &(cluster_members[read_idx]->measurements); + } +} + +// This function works for the case where the measurements were not written in Alignment +void FlowSpaceConsensusMaster::GetMeasurements(const vector& cluster_members, vector< vector >& flow_synchronized_measurements) +{ + flow_synchronized_measurements.reserve(cluster_members.size()); + for (vector::const_iterator read_it = cluster_members.begin(); read_it != cluster_members.end(); ++read_it) + { + vector quantized_measurements; + if (not (*read_it)->alignment.GetTag("ZM", quantized_measurements)) { + cerr << "ERROR: Normalized measurements ZM:tag is not present in read " << (*read_it)->alignment.Name << endl; + exit(1); + } + flow_synchronized_measurements.push_back(vector(quantized_measurements.size())); + for (unsigned int i_flow = 0; i_flow < quantized_measurements.size(); ++i_flow){ + flow_synchronized_measurements.back()[i_flow] = ((float) quantized_measurements[i_flow]) / 256.0f; + } + } +} + +// consensus_phase_params = mean of the phase_params +void FlowSpaceConsensusMaster::CalculateConsensusPhaseParams(const vector& cluster_members, vector& consensus_phase_params, bool is_zero_droop) +{ + consensus_phase_params.assign(3, 0.0f); + float num_reads = (float) cluster_members.size(); + assert(num_reads > 0.0f); + for (vector::const_iterator read_it = cluster_members.begin(); read_it != cluster_members.end(); ++read_it){ + consensus_phase_params[0] += ((*read_it)->phase_params[0]); + consensus_phase_params[1] += ((*read_it)->phase_params[1]); + } + consensus_phase_params[0] /= num_reads; + consensus_phase_params[1] /= num_reads; + + if (not is_zero_droop){ + for (vector::const_iterator read_it = cluster_members.begin(); read_it != cluster_members.end(); ++read_it){ + consensus_phase_params[2] += ((*read_it)->phase_params[2]); + } + consensus_phase_params[2] /= num_reads; + } +} + + +// Get the length of trimmed insert bases using the ZA tag where ZA = number of insert bases. +// Note that insert bases = template + YE + YT in 5.2. +// Note that insert bases = template in 5.2.1 and after. +// Input is_suffix_in_za = true if BaseCaller version <= 5.2; false if BaseCaller version >= 5.2.1 +int LenTrimmedInsertBases(const Alignment* rai, bool is_suffix_in_za) +{ + int num_trimmed_bases = 0; + int za = -1; + string ye = ""; + int ye_len = 0; + int len_QueryBases = (int) rai->alignment.QueryBases.size(); + + if( not rai->alignment.GetTag("ZA", za)){ + uint32_t u_za = 0; + if (not rai->alignment.GetTag("ZA", u_za)) { + cerr << "ERROR: ZA tag not found in read " << rai->alignment.Name << endl; + exit(1); + } + za = (int) u_za; + } + if (za < len_QueryBases){ + cerr << "Error: za < QueryBases.size() in read " << rai->alignment.Name << endl; + exit(1); + } + num_trimmed_bases = is_suffix_in_za? za - (len_QueryBases + rai->suffix_bases.size()) : za - len_QueryBases; + return num_trimmed_bases; +} + + +// Input is_suffix_in_za = true if BaseCaller version <= 5.2; false if BaseCaller version >= 5.2.1 +bool IsQualityTrimmedRead(const Alignment* rai, bool is_suffix_in_za){ + // It should be is_quality_trimmed = ( LenTrimmedInsertBases(rai) > 0) + // But the bases additionally trimmed by with heal-tag-indel-hp as also counted in LenTrimmedInsertBases(rai). + // The number 4 is safe against this issue. + int max_num_trimmed_bases_allowed = is_suffix_in_za? 4 : 0; + bool is_quality_trimmed = abs(LenTrimmedInsertBases(rai, is_suffix_in_za)) > max_num_trimmed_bases_allowed; + return is_quality_trimmed; +} + +// The return value indicates whether the consensus read needs realignment or not. +// Two cases where realignment is not needed +// (Case 1): The consensus query bases is exactly the same as the template read in the cluster. +// (Case 2): The consensus query bases all matches the reference. +// Usually, only a very small portion of consensus reads needs realignment. +bool FlowSpaceConsensusMaster::SaveToBamAlignment(const FlowSpaceCluster& my_cluster, + const vector& consensus_phase_params, + const vector& consensus_measurements, + const vector& measurements_sd, + const string& trimmed_bases, + const string& consensus_read_name, + int start_flow, + BamAlignment& alignment) +{ + // Use the read with the best mapping quality in the cluster as a template read. + // The tags "ZT", "ZE", "YT", "YE", "RG", etc. follow the template read. + // Note that The choice of the read name will affect the recaliration results when running tvc with the consensus bam. + const BamAlignment& template_alignment = my_cluster.cluster_members[my_cluster.best_mapping_quality_index]->alignment; + alignment = template_alignment; + + // Set the query bases + alignment.Name = consensus_read_name; + alignment.QueryBases = trimmed_bases; + alignment.Length = (int32_t) alignment.QueryBases.size(); + if (my_cluster.is_reverse_strand){ + RevComplementInPlace(alignment.QueryBases); + } + + // If the query bases of the consensus read is exactly the same as the template read, then I don't need to realign the consensus read. + bool need_realign = (alignment.QueryBases != template_alignment.QueryBases); + + // If not exactly the same as the template, then let's try another trivial alignment where the consensus query bases all matches the reference + if (need_realign){ + bool all_match_ref = false; + string template_md = ""; + template_alignment.GetTag("MD", template_md); + // If the template query bases all matches the reference, then no need to check again. + if ((template_md != to_string((int) template_alignment.QueryBases.size())) + or (alignment.QueryBases.size() != template_alignment.QueryBases.size())){ + long try_start_position = template_alignment.Position; + if (my_cluster.is_reverse_strand){ + int template_xm = (int) alignment.QueryBases.size(); // XM is the ion tag that specifies the number of ref bases spanned by the alignment. + template_alignment.GetTag("XM", template_xm); + // The actual "start" position of a reverse read of should be the end of the alignment. + try_start_position = template_alignment.Position + (long) template_xm - (long) alignment.QueryBases.size(); + } + + string reference_bases = reference_reader_->substr((int) (template_alignment.RefID), try_start_position, (long) alignment.QueryBases.size()); + all_match_ref = alignment.QueryBases == reference_bases; + + if (all_match_ref){ + need_realign = false; + alignment.Position = try_start_position; + alignment.Qualities = "*"; + // I set MapQ super sloppily by adding 3 (since from not all matches to all matches it means that the mapping quality is getting better). + // Also, according to the spec, no alignments should be assigned mapping quality 255. + alignment.MapQuality = (uint16_t) (max(254 - 3, (int) alignment.MapQuality) + 3); + alignment.CigarData.assign(1, CigarOp('M', (uint32_t) alignment.QueryBases.size())); // All-match cigar + alignment.EditTag("MD", "Z", to_string(alignment.QueryBases.size())); // All matches MD + alignment.EditTag("NM", "i", 0); // no mismatch + alignment.EditTag("XM", "i", (int) alignment.QueryBases.size()); + } + } + } + + if (need_realign){ + alignment.CigarData.assign(1, CigarOp('M', (uint32_t) alignment.QueryBases.size())); // dummay cigar that claim all M + alignment.Qualities = "*"; + alignment.MapQuality = 0; + alignment.RemoveTag("MD"); + alignment.RemoveTag("NM"); + alignment.RemoveTag("XM"); + } + + // Add/Edit/Remove tags + if (is_suffix_counted_in_za_){ + alignment.EditTag("ZA", "i", (int) (trimmed_bases.size() + my_cluster.trim_info.suffix_bases.size())); + }else{ + alignment.EditTag("ZA", "i", (int) trimmed_bases.size()); + } + alignment.EditTag("ZF", "i", start_flow); + alignment.EditTag("ZG", "i", my_cluster.adapter_start_flow); + alignment.EditTag("ZC", vector {my_cluster.adapter_start_flow, -1, -1, -1}); // ZC[1], ZC[2], ZC[3] may vary members from members, so I don't save them for the consensus reads. + alignment.EditTag("ZP", consensus_phase_params); + alignment.RemoveTag("ZB"); // ZB may vary members from members, so I don't save them for the consensus reads. + + // Add the ZM tag + vector vec_temp(consensus_measurements.size()); + for (unsigned int i_flow = 0; i_flow < consensus_measurements.size(); ++i_flow){ + vec_temp[i_flow] = (int16_t) (consensus_measurements[i_flow] * 256.0f); + } + alignment.EditTag("ZM", vec_temp); + + // New tags "ZR", "ZN", "ZS" from consensus + // Check the pre-existence of these tags. + for (vector::const_iterator read_it = my_cluster.cluster_members.begin(); read_it != my_cluster.cluster_members.end(); ++read_it) + { + if ((*read_it)->alignment.HasTag("ZN") or (*read_it)->alignment.HasTag("ZR") or (*read_it)->alignment.HasTag("ZS")){ + cerr << "Warning: The tag ZN, ZR or ZS is found in the read "<< (*read_it)->alignment.Name << ". " + << "The input bam file may contain unrecognized tags or may be a consensus bam. " + << "Some information may be lost or the ZM, ZP, ZS, ZR, ZN tags may be calculated incorrectly." << endl; + } + } + + vec_temp.resize(measurements_sd.size()); + for (unsigned int i_flow = 0; i_flow < measurements_sd.size(); ++i_flow){ + vec_temp[i_flow] = (int16_t) (measurements_sd[i_flow] * 256.0f); + } + alignment.EditTag("ZS", vec_temp); + + alignment.EditTag("ZR", "i", (int) my_cluster.cluster_members.size()); // ZR tag = read count, number of reads that form the consensus read + string read_names = ""; + for (unsigned int i_member = 0; i_member < my_cluster.cluster_members.size(); ++i_member){ + read_names += my_cluster.cluster_members[i_member]->alignment.Name; + if (i_member != my_cluster.cluster_members.size() - 1){ + read_names += ";"; + } + } + for (string::iterator c_it = read_names.begin(); c_it != read_names.end(); ++c_it) + if (*c_it == ':') {*c_it = '.';} // use "." to replace ":" + alignment.EditTag("ZN", "Z", read_names); // ZN tag = query names of the reads that from the consensus read + + return need_realign; +} + +bool CompareMapQ(const Alignment* const rai_1, const Alignment* const rai_2){ + return rai_1->alignment.MapQuality > rai_2->alignment.MapQuality; +} + +void AppendPositionTicket(list::iterator& position_ticket, Alignment* const alignment) +{ + if (position_ticket->begin == NULL) + position_ticket->begin = alignment; + if (position_ticket->end != NULL) + position_ticket->end->next = alignment; + position_ticket->end = alignment; + position_ticket->end->next = NULL; +} + +// Input: family_members +// Output: consensus_position_ticket, aln_needed_consensus_position_ticket +unsigned int FlowSpaceConsensusMaster::FlowSpaceConsensusOneFamily(vector& family_members, + list::iterator& consensus_position_ticket, + list::iterator& aln_needed_consensus_position_ticket) +{ + // For ConsensusCounter + static ConsensusCounter my_counter(consensus_for_molecular_tag); + + if (family_members.empty()){ + return 0; + } + + unsigned int add_to_num_func_fam = family_members.empty()? 0 : 1; + unsigned int add_to_num_consensus_reads = 0; + unsigned int add_to_num_single_read_consensus = 0; + unsigned int add_to_num_reads_in_func_fam = family_members.size(); + unsigned int add_to_num_reads_in_consensus_bam = 0; + unsigned int add_to_num_consensus_reads_need_aln = 0; + + vector flow_space_clusters; + vector isolated_reads; // reads w/o 3' adapter found or quality trimmed are isolate reads. + + // This should be enough most of the time. + if (consensus_for_molecular_tag){ + flow_space_clusters.reserve(4); + if ((not filter_single_read_consensus) and (not (need_3_end_adapter and filter_qt_reads))){ + isolated_reads.reserve(8); + } + }else{ + flow_space_clusters.reserve(32); + if ((not filter_single_read_consensus) and (not (need_3_end_adapter and filter_qt_reads))){ + isolated_reads.reserve(add_to_num_reads_in_func_fam / 8); + } + } + + // (Step 0): sort the reads by mapping quality + sort(family_members.begin(), family_members.end(), CompareMapQ); + + // (Step 1): Flow space clustering + for (vector::const_iterator member_it = family_members.begin(); member_it != family_members.end(); ++member_it) + { + const Alignment* rai = *member_it; // Now I do flowspace clustering for the read rai. + + // Did BaseCaller find 3' adapter in the read? + bool is_adapter_found = (rai->alignment.HasTag("ZC") and rai->alignment.HasTag("ZA")); + if (not is_adapter_found){ + if (not (need_3_end_adapter or filter_single_read_consensus)){ + isolated_reads.push_back(rai); + } + continue; + } + + // Is the read quality trimmed? + bool is_quality_trimmed_read = IsQualityTrimmedRead(rai, is_suffix_counted_in_za_); + if (is_quality_trimmed_read){ + if (not (filter_qt_reads or filter_single_read_consensus)){ + isolated_reads.push_back(rai); + } + continue; + } + + PrecomputeForClustering precomp_for_rai(rai); // The information in precomp_for_rai may be used frequently + bool is_in_existing_cluster = false; // Can I join any existing cluster + + for (vector::iterator cluster_it = flow_space_clusters.begin(); cluster_it != flow_space_clusters.end(); ++cluster_it){ + // Try to join any existing cluster if possible + if (cluster_it->AskToJoinMe(rai, precomp_for_rai)){ + is_in_existing_cluster = true; + break; // The read join the cluster. No need to seek joining others. + } + } + + if (not is_in_existing_cluster){ + // The read can not join any existing cluster. So it forms a new cluster. + flow_space_clusters.push_back(FlowSpaceCluster(rai, global_context_->flow_order_vector, precomp_for_rai)); + } + } + + // How many consensus reads will be generated for the reads in family_members? + add_to_num_consensus_reads = flow_space_clusters.size() + isolated_reads.size(); + add_to_num_reads_in_consensus_bam += isolated_reads.size(); + add_to_num_single_read_consensus += isolated_reads.size(); + + // (Step 2a): BamAlignment for isolate reads: no extra action required + for (vector::iterator read_it = isolated_reads.begin(); read_it != isolated_reads.end(); ++read_it) + { + // Append a new consensus alignment to consensus_position_ticket + Alignment* consensus_alignment = new Alignment; + consensus_alignment->alignment = (*read_it)->alignment; + AppendPositionTicket(consensus_position_ticket, consensus_alignment); + } + + // (Step 2b): BamAlignment for clusters + for (vector::iterator cluster_it = flow_space_clusters.begin(); cluster_it != flow_space_clusters.end(); ++cluster_it) + { + // The cluster consists of single read or no read, no additional action required + if (cluster_it->cluster_members.size() <= 1){ + if (cluster_it->cluster_members.empty() or filter_single_read_consensus){ + continue; + } + add_to_num_reads_in_consensus_bam += cluster_it->cluster_members.size(); + + // Append a new consensus alignment to consensus_position_ticket + Alignment* consensus_alignment = new Alignment; + consensus_alignment->alignment = cluster_it->cluster_members[0]->alignment; + AppendPositionTicket(consensus_position_ticket, consensus_alignment); + ++add_to_num_single_read_consensus; + continue; + } + add_to_num_reads_in_consensus_bam += cluster_it->cluster_members.size(); + + // Now I calculate the consensus_phase_params, consensus_measurements, trimmed_consensus_bases for the cluster + vector > measurements_in_cluster; + vector consensus_phase_params; // consensus phasing parameters for the cluster + vector consensus_measurements; // consensus measurements for the cluster + vector measurements_sd; // measurements deviation from consensus_measurements + string trimmed_consensus_bases; // prefix/suffix trimmed consensus base sequence as called by BaseCaller + int start_flow; // The first incorporated flow of trimmed_consensus_bases + + // Calculate consensus phase parameters and get the measurements of the reads + CalculateConsensusPhaseParams(cluster_it->cluster_members, consensus_phase_params, true); + + // Initialize BaseCaller + // Use the read with the best mapq as the template read of the cluster. + string consensus_read_name; + InitializeForBaseCalling(*cluster_it, consensus_phase_params, suppress_recalibration, consensus_read_name); + + // Calculate consensus measurements + GetMeasurements(cluster_it->cluster_members, measurements_in_cluster); + bool success = CalculateConsensusMeasurements(measurements_in_cluster, cluster_it->trim_info, consensus_measurements, measurements_sd); + + if (not success){ + cerr << "Warning: Fail to calculate the consensus measurements of the cluster ("; + for (vector::const_iterator read_it = cluster_it->cluster_members.begin(); read_it != cluster_it->cluster_members.end(); ++read_it){ + cerr << (*read_it)->alignment.Name <<", "; + } + cerr << "). The cluster will be ignored." << endl; + continue; + } + + // Trim prefix and suffix bases, note that trimmed_consensus_bases is the trimmed bases as called by BaseCaller + TrimPrefixSuffixBases(cluster_it->trim_info, trimmed_consensus_bases, start_flow); + + // Write to the consensus information to a new Alignment + Alignment* consensus_alignment = new Alignment; + bool aln_needed = SaveToBamAlignment(*cluster_it, consensus_phase_params, consensus_measurements, measurements_sd, trimmed_consensus_bases, consensus_read_name, start_flow, consensus_alignment->alignment); + + if (aln_needed){ + ++add_to_num_consensus_reads_need_aln; + // Append the new consensus alignment to consensus_position_ticket + AppendPositionTicket(aln_needed_consensus_position_ticket, consensus_alignment); + } + else{ + AppendPositionTicket(consensus_position_ticket, consensus_alignment); + } + } + my_counter.Count(add_to_num_func_fam, + add_to_num_consensus_reads, + add_to_num_single_read_consensus, + add_to_num_reads_in_func_fam, + add_to_num_reads_in_consensus_bam, + add_to_num_consensus_reads_need_aln); + return add_to_num_reads_in_consensus_bam; +} + +void FlowSpaceConsensusMaster::PropagateFlowspaceConsensusParameters(const ConsensusParameters& my_param, bool use_mol_tag){ + suppress_recalibration = my_param.program_flow.suppress_recalibration; + filter_qt_reads = my_param.filter_qt_reads; + filter_single_read_consensus = my_param.filter_single_read_consensus; + need_3_end_adapter = my_param.need_3_end_adapter; + consensus_for_molecular_tag = use_mol_tag; +} + +void FlowSpaceConsensusMaster::InitializeConsensusCounter(){ + // Initialize the consensus counter using dummy inputs since now I know consensus_for_molecular_tag. + list::iterator dummy_iter; + vector dummy_family(0); + FlowSpaceConsensusOneFamily(dummy_family, dummy_iter, dummy_iter); +} + +void GenerateFlowSpaceConsensusPositionTicket(vector< vector< vector > >& my_molecular_families_multisample, + FlowSpaceConsensusMaster& flow_space_consensus_master, + unsigned int min_family_size, + list::iterator& consensus_position_ticket, + list::iterator& aln_needed_consensus_position_ticket, + TargetsManager* targets_manager, + bool skip_consensus) +{ + // my_molecular_families_multisample is usually the famly pileup that cover one target. + // Typcally, a read just covers one target. + // map is a better container than vector to store the target stat. + // stat_of_targets[i] is the coverage information for the i-th unmerged region generated here. + // TODO: Should I split coverage stat for each sample? + map stat_of_targets; + for (vector< vector< vector< MolecularFamily> > >::iterator sample_it = my_molecular_families_multisample.begin(); sample_it != my_molecular_families_multisample.end(); ++sample_it) { + for (vector< vector< MolecularFamily> >::iterator strand_it = sample_it->begin(); strand_it != sample_it->end(); ++strand_it) { + for (vector< MolecularFamily>::iterator fam_it = strand_it->begin(); fam_it != strand_it->end(); ++fam_it) { + // Is *fam_it functional? + if (fam_it->SetFuncFromAll((unsigned int) min_family_size)) { + unsigned int consensus_fam_size = 0; + if (skip_consensus){ + consensus_fam_size = fam_it->GetFamSize(); + }else{ + // Generate consensus reads + consensus_fam_size = flow_space_consensus_master.FlowSpaceConsensusOneFamily(fam_it->all_family_members, consensus_position_ticket, aln_needed_consensus_position_ticket); + } + // Count the coverage of the target. + if (consensus_fam_size >= min_family_size and (not fam_it->all_family_members.empty())){ + // Important Assumption: is_split_families_by_region_ = true in MolecularFamilyGenerator. + for (vector::iterator target_it = fam_it->all_family_members[0]->target_coverage_indices.begin(); target_it != fam_it->all_family_members[0]->target_coverage_indices.end(); ++target_it){ + TargetStat& my_stat = stat_of_targets[*target_it]; + my_stat.read_coverage += consensus_fam_size; + ++(my_stat.family_coverage); + ++(my_stat.fam_size_hist[consensus_fam_size]); + } + } + } + } + } + } + targets_manager->AddCoverageToRegions(stat_of_targets); + +} diff --git a/Analysis/VariantCaller/Consensus/FlowSpaceConsensus.h b/Analysis/VariantCaller/Consensus/FlowSpaceConsensus.h new file mode 100644 index 00000000..aa5395d8 --- /dev/null +++ b/Analysis/VariantCaller/Consensus/FlowSpaceConsensus.h @@ -0,0 +1,138 @@ +/* Copyright (C) 2016 Thermo Fisher Scientific, Inc. All Rights Reserved */ + +#ifndef FLOWSPACECONSENSUS_H +#define FLOWSPACECONSENSUS_H + +#include +#include +#include +#include +#include +#include +#include +#include "InputStructures.h" +#include "BAMWalkerEngine.h" +#include "MolecularTag.h" +#include "ConsensusParameters.h" + +using namespace std; + +// ============================================================================== +// Pre-computed information for FlowSpaceCluster +struct PrecomputeForClustering +{ + PrecomputeForClustering(const Alignment* rai); + vector zc = {}; //! bug-fixed zc tag of the read + string base_seq = ""; //! base sequence as called by BaseCaller + vector flowgram = {}; //! flowgram of base_seq, will be computed if needed + vector base_index_to_flow_index = {}; //! the index mapping from base_seq to flowgram, will be computed if needed +}; + +// ============================================================================== + +struct TrimInfo +{ + string prefix_bases = ""; //! the 5' hard-clipped prefix bases (KS + ZT + ZE) + string suffix_bases = ""; //! the 3' hard-clipped suffix bases (YE + YT), 3' adapter not included + int prefix_end_flow = -1; //! Flow corresponding to to the last base of the 5' hard clipped prefix + int prefix_end_hp = -1; //! Last hp length of prefix_bases + int suffix_start_flow = -1; //! The first incorporated flow of the suffix_bases. Set to the end flow of template if no suffix_bases. + int suffix_start_hp = -1; //! First hp length of suffix_bases +}; + +// ============================================================================== + +//@TODO: optimize the heuristic assigned parameters kMaxNumDiffFlows_, kMaxNumLocalDiffFlows_, kStrictFlowSync_ +class FlowSpaceCluster +{ +private: + const static int kMaxNumDiffFlows_ = 6; //! Build-in parameter for bool IsFlowSynchronized(...) + const static int kMaxNumLocalDiffFlows_ = 2; //! Build-in parameter for bool IsFlowSynchronized(...) + const static bool kStrictFlowSync_ = false; //! Build-in parameter for bool IsFlowSynchronized(...) + const ion::FlowOrder* const flow_order_; //! the flow order corresponds to run_id + string template_base_seq_ = ""; //! The template base sequence that represents the cluster + vector template_flowgram_ = {}; //! The flowgram of template_base_seq_ + + void InitializeCluster_(const Alignment *new_rai); + void AddNewMember_(const Alignment* new_rai, PrecomputeForClustering& precomp_for_new_rai); + +public: + const string runid; //! Every read must come from the same run + const short flow_order_index; //! flow order index used by of the cluster + const bool is_reverse_strand; //! The reads in the cluster must be on the same strand. + int adapter_start_flow = -1; //! The ZG tag (or ZC[0]) of the read in Ion BAM format + int best_mapping_quality_index = -1; //! The index of the read in cluster_members that has the best mapping quality + uint16_t best_mapping_quality = 0; //! The best mapping quality of the reads in cluster_members + TrimInfo trim_info; //! Information for trimming prefix bases and suffix bases + vector cluster_members = {}; //! the read members in the cluster + + FlowSpaceCluster(const Alignment* new_rai, const ion::FlowOrder* fo); // Call me to construct an object if you want to ignore flow_order_index + FlowSpaceCluster(const Alignment* new_rai, const vector& flow_order_vector, PrecomputeForClustering& precomp_for_new_rai); + + bool AskToJoinMe(const Alignment* query_rai); + bool AskToJoinMe(const Alignment* query_rai, PrecomputeForClustering& precomp_for_query_rai); +}; + +// ============================================================================== + +class FlowSpaceConsensusMaster +{ +private: + short flow_order_index_; //! The flow order index associated with the reads that form the consensus + const ion::FlowOrder* flow_order_; //! Flow order specified by flow_order_index_ + BasecallerRead consensus_read_; //! Consensus flow space information + PersistingThreadObjects* const thread_objects_; //! Used to call Treephaser and the realigner + const ReferenceReader* const reference_reader_; //! Reference reader for realignemnt + const InputStructures* const global_context_; //! Header information of the bam file + bool is_suffix_counted_in_za_; //! true if the ZA tag counts the suffix bases (BaseCaller 5.2-0, ..., 5.2-20), else false. + +public: + bool filter_qt_reads = false; //! filtered out quality trimmed reads + bool need_3_end_adapter = false; //! filtered out the reads w/o 3' end adapter found + bool suppress_recalibration = true; //! suppress recalibration when solving the consensus measurements + bool consensus_for_molecular_tag = false; //! consensus for molecular tags? + bool filter_single_read_consensus = false; //! filtered out single-read consensus + FlowSpaceConsensusMaster(PersistingThreadObjects* thread_objects, + const ReferenceReader* reference_reader, + const InputStructures* global_context, + const string& basecaller_ver); + + void PropagateFlowspaceConsensusParameters(const ConsensusParameters& my_param, bool use_mol_tag); + void CalculateConsensusPhaseParams(const vector& cluster_members, vector& consensus_phase_params, bool is_zero_droop = true); + void GetMeasurements(const vector& cluster_members, vector *>& flow_synchronized_measurements_ptr); + void GetMeasurements(const vector& cluster_members, vector< vector >& flow_synchronized_measurements); + void InitializeForBaseCalling(const FlowSpaceCluster& my_cluster, const vector& consensus_phase_params, bool suppress_recal, string &consensus_read_name); + void InitializeConsensusCounter(); + bool CalculateConsensusMeasurements(const vector >& flow_synchronized_measurements, + const TrimInfo& trim_info, + vector& consensus_measurements, + vector& measurements_sd); + bool CalculateConsensusMeasurements(const vector *>& flow_synchronized_measurements_ptr, + const TrimInfo& trim_info, + vector& consensus_measurements, + vector& measurements_sd); + void TrimPrefixSuffixBases(const TrimInfo& trim_info, string& trimmed_consensus_bases, int& start_flow); + bool SaveToBamAlignment(const FlowSpaceCluster& my_cluster, + const vector& consensus_phase_params, + const vector& consensus_measurements, + const vector& measurements_sd, + const string& trimmed_bases, + const string& consensus_read_name, + int start_flow, + BamAlignment& alignment); + unsigned int FlowSpaceConsensusOneFamily(vector& family_members, + list::iterator& consensus_position_ticket, + list::iterator& aln_needed_consensus_position_ticket); +}; + +// ============================================================================== + +void GenerateFlowSpaceConsensusPositionTicket(vector< vector< vector > >& my_molecular_families_multisample, + FlowSpaceConsensusMaster& flow_space_consensus_master, + unsigned int min_family_size, + list::iterator& consensus_position_ticket, + list::iterator& aln_needed_consensus_position_ticket, + TargetsManager* targets_manager, + bool skip_consensus); + +#endif /* FLOWSPACECONSENSUS_H */ diff --git a/Analysis/VariantCaller/EnsembleEval/BiasGenerator.cpp b/Analysis/VariantCaller/EnsembleEval/BiasGenerator.cpp index a43cda6b..ea513293 100644 --- a/Analysis/VariantCaller/EnsembleEval/BiasGenerator.cpp +++ b/Analysis/VariantCaller/EnsembleEval/BiasGenerator.cpp @@ -2,9 +2,6 @@ #include "BiasGenerator.h" - - - // bias generator handles latent variables representing sources of bias in measurement // the trivial example is by strand void BasicBiasGenerator::GenerateBiasByStrand(int i_hyp, HiddenBasis &delta_state, vector &test_flow, int strand_key, vector &new_residuals, vector &new_predictions){ @@ -53,7 +50,7 @@ void BasicBiasGenerator::AddOneUpdate(HiddenBasis &delta_state, const vector 1){ + cout << " + Latent bias"<< (print_updated? " updated:" : ":") << endl + << " - FWD: latent_bias = " << PrintIteratorToString(latent_bias[0].begin(), latent_bias[0].end()) <0.0f) { - l_sigma = sigma*skew; - } else { - l_sigma = sigma/skew; - } - - float x = res/l_sigma; - float xx = x*x; - float v = 2*half_n-1; // 1,3,5,7,... - float my_likelihood = 1.0f/(3.14159f*sqrt(v)); - float my_factor = 1.0f/(1.0f+xx/v); - - for (int i_prod=0; i_prod 0.0f)? sigma * skew : sigma / skew; + } + float x = res / l_sigma; + float my_log_likelihood = log_factor; + + my_log_likelihood += half_n * (log_v - log(v + x * x)); + my_log_likelihood -= log(l_sigma); + if (skew == 1.0f){ + return my_log_likelihood; + } + // account for skew + float skew_factor = log(2.0f*skew/(skew*skew+1.0f)); + my_log_likelihood += skew_factor; + return my_log_likelihood; } HiddenBasis::HiddenBasis(){ @@ -105,6 +98,7 @@ void HiddenBasis::Allocate(unsigned int num_hyp, unsigned int num_test_flow){ void CrossHypotheses::CleanAllocate(int num_hyp, int num_flow) { // allocate my vectors here responsibility.assign(num_hyp, 0.0f); + weighted_responsibility.assign(num_hyp, 0.0f); log_likelihood.assign(num_hyp, 0.0f); scaled_likelihood.assign(num_hyp, 0.0f); @@ -115,9 +109,10 @@ void CrossHypotheses::CleanAllocate(int num_hyp, int num_flow) { predictions_all_flows.resize(num_hyp); mod_predictions.resize(num_hyp); normalized_all_flows.assign(num_flow, 0.0f); + measurement_sd_all_flows.assign(num_flow, 0.0f); residuals.resize(num_hyp); sigma_estimate.resize(num_hyp); - basic_likelihoods.resize(num_hyp); + basic_log_likelihoods.resize(num_hyp); for (int i_hyp=0; i_hyp 1){ + measurement_sd_all_flows = my_read.measurements_sd; + } + for (unsigned int i_hyp = 1; i_hyp < same_as_null_hypothesis.size(); ++i_hyp){ + at_least_one_same_as_null += same_as_null_hypothesis[i_hyp]; + } } void CrossHypotheses::InitializeTestFlows() { @@ -173,7 +184,7 @@ void CrossHypotheses::InitializeRelevantToTestFlows(){ mod_predictions[i_hyp].assign(test_flow_num, 0.0f); residuals[i_hyp].assign(test_flow_num, 0.0f); sigma_estimate[i_hyp].assign(test_flow_num, 0.0f); - basic_likelihoods[i_hyp].assign(test_flow_num, 0.0f); + basic_log_likelihoods[i_hyp].assign(test_flow_num, 0.0f); } normalized.assign(test_flow_num, 0.0f); for(unsigned int t_flow = 0; t_flow < test_flow_num; ++t_flow){ @@ -185,14 +196,19 @@ void CrossHypotheses::InitializeRelevantToTestFlows(){ } delta_state.Allocate(num_hyp, test_flow_num); - // clear the data of all flows if we don't want to preserve it - if(not preserve_full_data){ - normalized_all_flows.clear(); - predictions_all_flows.clear(); + // If it is a consensus read + if (read_counter > 1){ + // Note that if the ZS tag is not presented, I set measurement_sd to be all zeros. + measurement_var.assign(test_flow_num, 0.0f); + for(unsigned int t_flow = 0; t_flow < test_flow_num; ++t_flow){ + int j_flow = test_flow[t_flow]; + if (j_flow < (int) measurement_sd_all_flows.size()){ + measurement_var[t_flow] = measurement_sd_all_flows[j_flow] * measurement_sd_all_flows[j_flow]; + } + } } } - void CrossHypotheses::InitializeDerivedQualities() { InitializeResponsibility(); // depends on hypotheses @@ -203,16 +219,21 @@ void CrossHypotheses::InitializeDerivedQualities() { InitializeSigma(); // depends on predicted my_t.SetV(heavy_tailed); + // 2*heavy_tailed - 1 = Dof of t-dist + sigma_factor = adjust_sigma? sqrt((2.0f * heavy_tailed - 3.0f) / (2.0f * heavy_tailed - 1.0f)) : 1.0f; - ComputeBasicLikelihoods(); // depends on residuals and sigma + ComputeBasicLogLikelihoods(); // depends on residuals and sigma // compute log-likelihoods ComputeLogLikelihoods(); // depends on test flow(s) } void CrossHypotheses::InitializeResponsibility() { responsibility[0] = 1.0f; // everyone is an outlier until we trust you - for (unsigned int i_hyp=1; i_hyp &hyp_prob, float typical_prob) { - +void CrossHypotheses::UpdateResponsibility(const vector &hyp_prob, float outlier_prob) { + if (at_least_one_same_as_null){ + // In principle, an outlier read means it supports neither alleles. + // In this case, the read can't be an outlier because the sequence as called is the same as an allele. + // Hence I override outlier_prob to be extremely low. + // Otherwise, the reads that supports the allele with AF < outlier prob will be treated as outliers. + // This step can mitigate the bad parameter setting where min-allele-freq < outlier prob or the true AF ~ or < outlier prob + // while it does not affect the outlier handling of the read that is a indeed an outlier. + outlier_prob = min(outlier_prob, MINIMUM_RELATIVE_OUTLIER_PROBABILITY); + } + float typical_prob = 1.0f - outlier_prob; if (!success){ //cout << "alert: fail to splice still called" << endl; InitializeResponsibility(); } else { // vector tmp_prob(3); - tmp_prob_d[0] = (1.0f-typical_prob)*scaled_likelihood[0]; // i'm an outlier + tmp_prob_d[0] = outlier_prob * scaled_likelihood[0]; // i'm an outlier for (unsigned int i_hyp=1; i_hyp &hyp_prob, float ll_denom += tmp_prob_d[i_hyp]; } - for (unsigned int i_hyp=0; i_hyp &hyp_prob, float typical_prob) { - // vector tmp_prob(3); - tmp_prob_f[0] = (1.0f-typical_prob)*scaled_likelihood[0]; // i'm an outlier +float CrossHypotheses::ComputePosteriorLikelihood(const vector &hyp_prob, float outlier_prob) { + if (at_least_one_same_as_null){ + // In principle, an outlier read means it supports neither alleles. + // In this case, the read can't be an outlier because the sequence as called is the same as an allele. + // Hence I override outlier_prob to be extremely low. + // Otherwise, the reads that supports the allele with AF < outlier prob will be treated as outliers. + // This step can mitigate the bad parameter setting where min-allele-freq < outlier prob or the true AF ~ or < outlier prob + // while it does not affect the outlier handling of the read that is a indeed an outlier. + outlier_prob = min(outlier_prob, MINIMUM_RELATIVE_OUTLIER_PROBABILITY); + } + float typical_prob = 1.0f - outlier_prob; + tmp_prob_f[0] = outlier_prob * scaled_likelihood[0]; // i'm an outlier for (unsigned int i_hyp=1; i_hyp 0.0f) ? sqrt(adj_res * adj_res + measurement_var[t_flow]) : -sqrt(adj_res * adj_res + measurement_var[t_flow]); + } + float my_sigma = adjust_sigma? sigma_estimate[i_hyp][t_flow] * sigma_factor : sigma_estimate[i_hyp][t_flow]; + basic_log_likelihoods[i_hyp][t_flow] = my_t.LogTDistOddN(adj_res, my_sigma, skew_estimate); // pure observational likelihood depends on residual + current estimated sigma under each hypothesis + } + } + } } void CrossHypotheses::UpdateRelevantLikelihoods() { - ComputeBasicLikelihoods(); + ComputeBasicLogLikelihoods(); ComputeLogLikelihoods(); // automatically over relevant likelihoods } @@ -423,7 +478,10 @@ void CrossHypotheses::ComputeLogLikelihoodsSum() { for (unsigned int i_hyp=0; i_hyp 1){ + log_likelihood[i_hyp] *= read_counter_f; } } } @@ -451,7 +509,24 @@ void CrossHypotheses::JointLogLikelihood() { float sigma_projection = 0.001f; // always some minimal variance in case we divide for (unsigned int t_flow = 0; t_flow 0)? + sqrt(residuals[i_hyp][t_flow] * residuals[i_hyp][t_flow] + measurement_var[t_flow]) : -sqrt(residuals[i_hyp][t_flow] * residuals[i_hyp][t_flow] + measurement_var[t_flow]); + } + res_component = adj_res * d_val/ delta_scale; + } + + + res_projection += res_component; /*for (unsigned int s_flow=0; s_flow0) with the prior P(hyp_i) = typical_prob. -// I claim the read is "not" an outlier, if I find any binary hypothesis pair hyp_0 vs. hyp_i s.t. log APP(hyp_0) < log APP(hyp_i), -// where APP is the posterior probability. -// Suppose that sigma is the default magic sigma (minimum_sigma_prior = 0.085, slope_sigma_prior = 0.0084). -// Example 1: -// hyp_0: 0-mer, hyp_1: 1-mer, and the measurement = 0-mer -// If outlier_probability < 0.00055 then we claim that it is not an outlier. -// => We need outlier_probability < 5.5E-4 to tolerate a 1-mer error at a single flow. -// Example 2: -// Suppose hyp_0: 0-mer, hyp_1: 2-mer, and the measurement = 0-mer -// With the default value of magic sigma, if outlier_probability < 1.1E-5 then we claim that it is not an outlier. -// => We need outlier_probability < 1.1E-5 to tolerate a 2-mer error at a single flow. -// Example 3: -// Suppose hyp_0: {0-mer, 0-mer}, hyp_1: {1-mer, 1-mer}, and the measurement = {0-mer, 0-mer} -// With the default value of magic sigma, if outlier_probability < 3.1E-7 then we claim that it is not an outlier. -// => We need outlier_probability < 3.1E-7 to tolerate 1-mer, 1-mer errors at two flows. -bool CrossHypotheses::LocalOutlierClassifier(float typical_prob){ - //Use CheckParameterLowerUpperBound(...) to prevent extremely low ol_prob instead - //if(ol_prob < MINIMUM_RELATIVE_OUTLIER_PROBABILITY){ - // ol_prob = MINIMUM_RELATIVE_OUTLIER_PROBABILITY; - // typical_prob = 1.0f - MINIMUM_RELATIVE_OUTLIER_PROBABILITY; - //} - bool is_outlier = true; - - // First check same_as_null_hypothesis - // The read is not an outlier if any hypothesis not null is the same as the null hypothesis - // This can prevent the case where we classify a hypothesis the same as null to be an outlier if typical_prob < 0.5. - for(unsigned int i_hyp = 1; i_hyp < same_as_null_hypothesis.size(); ++i_hyp){ - if(same_as_null_hypothesis[i_hyp]){ - is_outlier = false; - return is_outlier; - } - } - - float log_priori_not_ol = log(typical_prob); - float log_posterior_likelihood_ol = log(1.0f - typical_prob) + log_likelihood[0]; - - for(unsigned int i_hyp = 1; i_hyp < log_likelihood.size(); ++i_hyp){ - float log_posterior_likelihood_not_ol = log_priori_not_ol + log_likelihood[i_hyp]; - // If any hypothesis says "I am not an outlier", then the read is not an outlier read. - if(log_posterior_likelihood_ol < log_posterior_likelihood_not_ol){ - is_outlier = false; - return is_outlier; - } - } - return is_outlier; -} - - - void EvalFamily::InitializeEvalFamily(unsigned int num_hyp){ - ResetFamily(); CleanAllocate(num_hyp); InitializeFamilyResponsibility(); } @@ -664,6 +692,7 @@ void EvalFamily::InitializeEvalFamily(unsigned int num_hyp){ void EvalFamily::CleanAllocate(unsigned int num_hyp){ // I only clean and allocate the ones that I need. my_family_cross_.responsibility.assign(num_hyp, 0.0f); + my_family_cross_.weighted_responsibility.assign(num_hyp, 0.0f); family_responsibility.assign(num_hyp, 0.0f); my_family_cross_.log_likelihood.assign(num_hyp, 0.0f); my_family_cross_.scaled_likelihood.assign(num_hyp, 0.0f); @@ -680,34 +709,394 @@ void EvalFamily::InitializeFamilyResponsibility(){ } } -// Currently, I don't handle the case of "outlier family". -// I force the log-likelihood of the outlier hypothesis of each family to be extremely low. -// So no family will be treated as an outlier. -// Do ShortStack::OutlierFiltering() before barcode classification to make sure that all families consist of no outlier read. -// @TODO: Perhaps need a better way to deal with outlier families? void EvalFamily::ComputeFamilyLogLikelihoods(const vector &my_hypotheses){ - my_family_cross_.log_likelihood[0] = -9999.9f; // no other hypothesis would have log-likelihood less than this value + my_family_cross_.log_likelihood[0] = -999999.9f; // accumulate the log-likelihood from the reads of the family for not null hypotheses - for (unsigned int i_hyp=1; i_hyp < my_family_cross_.log_likelihood.size(); i_hyp++) { + for (unsigned int i_hyp = 1 ; i_hyp < my_family_cross_.log_likelihood.size(); i_hyp++) { my_family_cross_.log_likelihood[i_hyp] = 0.0f; - for (unsigned int i_member = 0; i_member < family_members.size(); ++i_member){ - unsigned int i_read = family_members[i_member]; - my_family_cross_.log_likelihood[i_hyp] += my_hypotheses[i_read].log_likelihood[i_hyp]; + for (unsigned int i_member = 0; i_member < valid_family_members.size(); ++i_member){ + unsigned int i_read = valid_family_members[i_member]; + my_family_cross_.log_likelihood[i_hyp] += ((1.0f - my_hypotheses[i_read].responsibility[0]) * my_hypotheses[i_read].log_likelihood[i_hyp]); } } my_family_cross_.ComputeScaledLikelihood(); } // ComputeFamilyLogLikelihoods(...) must be done first! -void EvalFamily::UpdateFamilyResponsibility(const vector &hyp_prob, float typical_prob){ - my_family_cross_.UpdateResponsibility(hyp_prob, typical_prob); +void EvalFamily::UpdateFamilyResponsibility(const vector &hyp_prob, float outlier_prob){ + my_family_cross_.UpdateResponsibility(hyp_prob, outlier_prob); family_responsibility = my_family_cross_.responsibility; } -float EvalFamily::ComputeFamilyPosteriorLikelihood(const vector &hyp_prob, float typical_prob){ - return my_family_cross_.ComputePosteriorLikelihood(hyp_prob, typical_prob); +// (Note 1): my_family_cross_.log_likelihood[0] is set to be super low +// (Note 2): family_responsibility[0] is obtained using an ad hoc fashion (not derived from family log-likelihood) +// So I can't calculate the family posterior likelihood from scaled_likelihood[0]. +// I kind of reversely engineering from family_responsibility[0] to family posterior likelihood +float EvalFamily::ComputeFamilyPosteriorLikelihood(const vector &hyp_prob){ + float ll_denom = 0.0f; + float safety_zero = 1.0E-12; + + for (unsigned int i_hyp = 1; i_hyp < my_family_cross_.scaled_likelihood.size(); ++i_hyp){ + ll_denom += hyp_prob[i_hyp - 1] * my_family_cross_.scaled_likelihood[i_hyp]; + } + ll_denom *= (1.0f - family_responsibility[0]); + // If family_responsibility[0] is high, FamilyPosteriorLikelihood is dominated by family_responsibility[0], i.e., not a function of hyp_prob. + ll_denom += family_responsibility[0]; + return log(ll_denom + safety_zero) + my_family_cross_.ll_scale; // log-likelihood under current distribution, including common value of log-likelihood-scale } int EvalFamily::MostResponsible(){ return my_family_cross_.MostResponsible(); } + +int EvalFamily::CountFamSizeFromAll() +{ + fam_size_ = 0; + for (vector::iterator read_it = all_family_members.begin(); read_it != all_family_members.end(); ++read_it){ + fam_size_ += (read_stack_->at(*read_it)->read_count); + } + return fam_size_; +} + +int EvalFamily::CountFamSizeFromValid() +{ + valid_fam_size_ = 0; + for (vector::iterator read_it = valid_family_members.begin(); read_it != valid_family_members.end(); ++read_it){ + valid_fam_size_ += (read_stack_->at(*read_it)->read_count); + } + return valid_fam_size_; +} + +// I define Family outlier responsibility = P(# non-outlier read members < min_fam_size) +// In theory, Resp(OL family) is obtained from the cdf of a multinomial distribution. +// Since Resp(OL family) >= min Resp(OL read), I use the lower bound to approximate Resp(OL family) for some extreme cases. +// Otherwise, I calculate Resp(OL family) via Monte-Carlo simulation. +// (Note 1): my_cross_.responsibility is not derived from my_cross_.log_likelihood in this function. +void EvalFamily::ComputeFamilyOutlierResponsibility(const vector &my_hypotheses, unsigned int min_fam_size) +{ + float family_ol_resp = 1.0f; + float safety_zero = 0.000001f; + float min_read_ol_resp = 1.0f; + float max_read_ol_resp = 0.0f; + float weighted_avg_read_ol_resp = 0.0f; + + int semi_hard_fam_size = 0; + + vector potential_outlier_reads; + + for (vector::iterator read_idx_it = valid_family_members.begin(); read_idx_it != valid_family_members.end(); ++read_idx_it){ + min_read_ol_resp = min(min_read_ol_resp, my_hypotheses[*read_idx_it].responsibility[0]); + max_read_ol_resp = max(max_read_ol_resp, my_hypotheses[*read_idx_it].responsibility[0]); + weighted_avg_read_ol_resp += (my_hypotheses[*read_idx_it].responsibility[0] * my_hypotheses[*read_idx_it].read_counter_f); + // The following criterion basically claims that the read is not an outlier. + if (my_hypotheses[*read_idx_it].at_least_one_same_as_null){ + // This condition basically implies that a hypothesis is the same as null. So it can't be an outlier. + semi_hard_fam_size += my_hypotheses[*read_idx_it].read_counter; + } + else{ + potential_outlier_reads.push_back(*read_idx_it); + } + } + int num_fam_needed = (int) min_fam_size - semi_hard_fam_size; + + if (semi_hard_fam_size >= (int) min_fam_size){ + // I am very sure that the the family consists of sufficient number of non-outlier reads (which is the most ubiquitous case). + // Don't waste time doing Monte Carlo simulation. Set family_ol_resp to close to zero. + family_ol_resp = safety_zero; + } + else if (min_read_ol_resp >= 0.8f){ + //@TODO: Come up with something better than the hard-coded threshold. + // It seems that all reads in the family are very likely to be outliers. + // Don't waste time doing Monte Carlo simulation. Use the averaged read_ol_reso as family_ol_resp. + family_ol_resp = weighted_avg_read_ol_resp / (float) GetValidFamSize(); + } + else if (potential_outlier_reads.size() == 1){ + // Another trivial case where I don't need to go through Monte Carlo. + family_ol_resp = my_hypotheses[potential_outlier_reads[0]].responsibility[0]; + } + else{ + // I calculate family outlier responsibility using Monte Carlo simulation. + RandSchrange RandGen(1729); // I choose 1729 as the seed because it is the Hardy–Ramanujan number. + int num_trails = 200; + int num_ol = 0; + // Only deal with those potential outlier reads. + vector min_rand_for_non_ol(potential_outlier_reads.size()); + for (unsigned int read_idx = 0; read_idx < min_rand_for_non_ol.size(); ++read_idx){ + min_rand_for_non_ol[read_idx] = (int) ((double)(RandGen.RandMax) * (double) my_hypotheses[potential_outlier_reads[read_idx]].responsibility[0]); + } + + for (int trail_round = 0; trail_round < num_trails; ++trail_round){ + int trailed_fam_size = 0; + for (unsigned int read_idx = 0; read_idx < min_rand_for_non_ol.size(); ++read_idx){ + // Toss a coin: head = outlier, tail = not outlier + bool is_not_ol = RandGen.Rand() > min_rand_for_non_ol[read_idx]; + if (is_not_ol){ + trailed_fam_size += my_hypotheses[potential_outlier_reads[read_idx]].read_counter; + } + } + num_ol += (trailed_fam_size < num_fam_needed); + } + family_ol_resp = (float) num_ol / (float) num_trails; + } + + // Guard by safety zero in case something crazy happened + family_ol_resp = max(family_ol_resp, safety_zero); + family_ol_resp = min(family_ol_resp, 1.0f - safety_zero); + + // Normalize the family responsibility + float sum_of_resp = family_ol_resp; + float normalization_factor = (1.0f - family_ol_resp) / (1.0f - my_family_cross_.responsibility[0]); + my_family_cross_.responsibility[0] = family_ol_resp; + for (unsigned int i_hyp = 1; i_hyp < my_family_cross_.responsibility.size(); ++i_hyp){ + my_family_cross_.responsibility[i_hyp] *= normalization_factor; + sum_of_resp += my_family_cross_.responsibility[i_hyp]; + } + family_responsibility = my_family_cross_.responsibility; + assert(sum_of_resp > 0.9999f and sum_of_resp < 1.0001f); +} + + +void RemoveHp(const string& base_seq, string& hp_removed_base_seq){ + hp_removed_base_seq.resize(0); + hp_removed_base_seq.reserve(base_seq.size()); + if (base_seq.empty()){ + return; + } + for (string::const_iterator nuc_it = base_seq.begin(); nuc_it != base_seq.end(); ++nuc_it){ + if (*nuc_it != hp_removed_base_seq.back()){ + hp_removed_base_seq.push_back(*nuc_it); + } + } +} + +bool IsHpIndel(const string& seq_1, const string& seq_2) +{ + if (seq_1.empty() or seq_2.empty()){ + return false; + } + + bool hp_indel_found = false; + int hp_len_1 = 0; + int hp_len_2 = 0; + string::const_iterator nuc_it_1 = seq_1.begin(); + string::const_iterator nuc_it_2 = seq_2.begin(); + while (nuc_it_1 != seq_1.end() or nuc_it_2 != seq_2.end()){ + if (*nuc_it_1 != *nuc_it_2){ + return false; + } + ++nuc_it_1; + ++nuc_it_2; + hp_len_1 = 1; + hp_len_2 = 1; + while (*nuc_it_1 == *(nuc_it_1 -1)){ + ++hp_len_1; + ++nuc_it_1; + } + while (*nuc_it_2 == *(nuc_it_2 -1)){ + ++hp_len_2; + ++nuc_it_2; + } + if (hp_len_1 != hp_len_2){ + if (hp_indel_found){ + return false; + } + hp_indel_found = true; + } + } + return true; +} + +// The function is implemented based on the assumption that instance_of_read_by_state is obtained from "splicing", +// I claim instance_of_read_by_state[i_hyp] and instance_of_read_by_state[j_hyp] are non-flow-disruptive if the first common suffix bases of the two hypotheses are mainly incorporated at the same flow. +void CrossHypotheses::FillInFlowDisruptivenessMatrix(const ion::FlowOrder &flow_order, const Alignment &my_read) +{ + // Every hypotheses pair starts with indefinite + local_flow_disruptiveness_matrix.assign(instance_of_read_by_state.size(), vector(instance_of_read_by_state.size(), -1)); + + if (not success){ + return; + } + + int common_prefix_len = -1; // length of common starting bases of all hypotheses. What I mean prefix here is NOT my_read.prefix_bases. It is the common starting bases used in splicing. + int min_instance_of_read_by_state_len = (int) instance_of_read_by_state[0].size(); + for (unsigned int i_hyp = 1; i_hyp < instance_of_read_by_state.size(); ++i_hyp){ + if ((int) instance_of_read_by_state[i_hyp].size() < min_instance_of_read_by_state_len){ + min_instance_of_read_by_state_len = (int) instance_of_read_by_state[i_hyp].size(); + } + } + + // Find the length of common starting bases for all hypotheses + int base_idx = 0; + while (common_prefix_len < 0 and base_idx < min_instance_of_read_by_state_len){ + for (unsigned int i_hyp = 1; i_hyp < instance_of_read_by_state.size(); ++i_hyp){ + if (instance_of_read_by_state[0][base_idx] != instance_of_read_by_state[i_hyp][base_idx]){ + common_prefix_len = base_idx; + break; + } + } + ++base_idx; + } + + // Check if I didn't see any delta, e.g., variant at the end of the read. + if (common_prefix_len <= 0){ + common_prefix_len = min_instance_of_read_by_state_len; + } + + char anchor_base = 0; // anchor_base is the last common prefix base of all hypotheses + int flow_index_of_anchor_base = my_read.start_flow; + if (common_prefix_len == 0){ + anchor_base = my_read.prefix_bases.back(); + // Find the flow index of anchor_base + while (flow_index_of_anchor_base >= 0 and flow_order.nuc_at(flow_index_of_anchor_base) != anchor_base){ + --flow_index_of_anchor_base; + } + } + else{ + anchor_base = instance_of_read_by_state[0][common_prefix_len - 1]; + flow_index_of_anchor_base = my_read.flow_index[common_prefix_len - 1]; + } + vector > flow_order_index_start_from_anchor; // flow_order index for each hypothesis, starting from the anchor base + // i.e., flow_order_index_start_from_anchor[i_hyp][idx] = the index of the main incorporating flow of instance_of_read_by_state[i_hyp][idx + common_prefix_len - 1] + flow_order_index_start_from_anchor.assign(instance_of_read_by_state.size(), {flow_index_of_anchor_base}); + + for (unsigned int i_hyp = 0; i_hyp < instance_of_read_by_state.size(); ++i_hyp){ + local_flow_disruptiveness_matrix[i_hyp][i_hyp] = 0; // identical means INDEL length 0. + for (unsigned int j_hyp = i_hyp + 1; j_hyp < instance_of_read_by_state.size(); ++j_hyp){ + if (i_hyp == 0 and same_as_null_hypothesis[j_hyp]){ + // same_as_null_hypothesis means identical + local_flow_disruptiveness_matrix[0][j_hyp] = 0; + local_flow_disruptiveness_matrix[j_hyp][0] = 0; + continue; + } + // determine the common_prefix_len for i_hyp, j_hyp + int common_prefix_len_i_j_pair = common_prefix_len; + while (instance_of_read_by_state[i_hyp][common_prefix_len_i_j_pair] == instance_of_read_by_state[j_hyp][common_prefix_len_i_j_pair]){ + ++common_prefix_len_i_j_pair; + if (common_prefix_len_i_j_pair >= (int) min(instance_of_read_by_state[i_hyp].size(), instance_of_read_by_state[j_hyp].size())) + break; + } + + // determine the common_suffix_len for i_hyp, j_hyp + // i_idx + 1, j_idx + 1 are the indices of the first common suffix base of i_hyp, j_hyp, respectively. + int i_idx = (int) instance_of_read_by_state[i_hyp].size() - 1; + int j_idx = (int) instance_of_read_by_state[j_hyp].size() - 1; + int common_suffix_len = 0; // The number of common ending bases of instance_of_read_by_state[i_hyp] and instance_of_read_by_state[j_hyp] + while ((min(i_idx, j_idx) > common_prefix_len_i_j_pair) and instance_of_read_by_state[i_hyp][i_idx] == instance_of_read_by_state[j_hyp][j_idx]){ + --i_idx; + --j_idx; + ++common_suffix_len; + } + + if (common_suffix_len == 0){ + // The flow-disruptiveness is indefinite because there is no common suffix bases (or may be hard-clipped). + // For example, the variant position is at the end of the read. + //@TODO: Append or use the suffix bases (if any, e.g., suffix molecular tag) if there is no other hard-clipped base (Usually, it is safe if --trim-ampliseq-primers=on). + continue; + } + + // Check HP-INDEL first because it is the easiest (and probably the most ubiquitous) one. + if (IsHpIndel(instance_of_read_by_state[i_hyp].substr(common_prefix_len_i_j_pair - 1, i_idx + 3 - common_prefix_len_i_j_pair), + instance_of_read_by_state[j_hyp].substr(common_prefix_len_i_j_pair - 1, j_idx + 3 - common_prefix_len_i_j_pair))){ + local_flow_disruptiveness_matrix[i_hyp][j_hyp] = 0; + local_flow_disruptiveness_matrix[j_hyp][i_hyp] = 0; + continue; + } + + // Now fill flow_order_index_start_from_anchor since I am here (not HP-INDEL). + int flow_i_hyp = flow_order_index_start_from_anchor[i_hyp].back(); + for (int base_idx = (int) flow_order_index_start_from_anchor[i_hyp].size() + common_prefix_len - 1; base_idx <= i_idx + 1; ++base_idx){ + IncrementFlow(flow_order, instance_of_read_by_state[i_hyp][base_idx], flow_i_hyp); + flow_order_index_start_from_anchor[i_hyp].push_back(flow_i_hyp); + } + int flow_j_hyp = flow_order_index_start_from_anchor[j_hyp].back(); + for (int base_idx = (int) flow_order_index_start_from_anchor[j_hyp].size() + common_prefix_len - 1; base_idx <= j_idx + 1; ++base_idx){ + IncrementFlow(flow_order, instance_of_read_by_state[j_hyp][base_idx], flow_j_hyp); + flow_order_index_start_from_anchor[j_hyp].push_back(flow_j_hyp); + } + + bool is_i_j_fd = flow_order_index_start_from_anchor[i_hyp][i_idx + 2 - common_prefix_len] != flow_order_index_start_from_anchor[j_hyp][j_idx + 2 - common_prefix_len]; + + if ((not is_i_j_fd) and flow_order_index_start_from_anchor[i_hyp][i_idx + 2 - common_prefix_len] == flow_order.num_flows()){ + // The flow order is not long enough to represent the first common suffix base for both i_hyp and j_hyp. So the flow-disruptiveness is indefinite. + continue; + } + + // Compare the index of the main incorporating flows of the first common suffix bases of the two hypotheses + if (is_i_j_fd){ + local_flow_disruptiveness_matrix[i_hyp][j_hyp] = 2; + local_flow_disruptiveness_matrix[j_hyp][i_hyp] = 2; + } + else{ + // Not FD and not HP-INDEL + local_flow_disruptiveness_matrix[i_hyp][j_hyp] = 1; + local_flow_disruptiveness_matrix[j_hyp][i_hyp] = 1; + } + } + } +} + +// A simple criterion to determine outlier reads +// If I can not find any non-null hypothesis which is not flow-disruptive with the null hypothesis, I claim the read is an outluer and return true. +bool CrossHypotheses::OutlierByFlowDisruptiveness() const { + if (not success or local_flow_disruptiveness_matrix.empty()){ + return true; + } + + for (unsigned int i_hyp = 1; i_hyp < local_flow_disruptiveness_matrix[0].size(); ++i_hyp){ + if (local_flow_disruptiveness_matrix[0][i_hyp] == 0 or local_flow_disruptiveness_matrix[0][i_hyp] == 1){ + return false; + } + } + // indefinite or flow-disruptive means outlier + return true; +} + +void EvalFamily::FillInFlowDisruptivenessMatrix(const vector &my_hypotheses) +{ + unsigned int num_hyp = 0; + for(unsigned int i_member = 0; i_member < all_family_members.size(); ++i_member){ + if (my_hypotheses[all_family_members[i_member]].success){ + num_hyp = my_hypotheses[all_family_members[i_member]].instance_of_read_by_state.size(); + break; + } + } + if (num_hyp == 0){ + my_family_cross_.local_flow_disruptiveness_matrix.clear(); + return; + } + + my_family_cross_.local_flow_disruptiveness_matrix.assign(num_hyp, vector(num_hyp, -1)); + // flow_disruptiveness_matrix[0][i_hyp] and flow_disruptiveness_matrix[i_hyp][0] are indefinite. + for (unsigned int i_hyp = 1; i_hyp < num_hyp; ++i_hyp){ + my_family_cross_.local_flow_disruptiveness_matrix[i_hyp][i_hyp] = 0; + for (unsigned int j_hyp = i_hyp + 1; j_hyp < num_hyp; ++j_hyp){ + // Note that loacal_flow_disruptiveness_matrix[i_hyp][j_hyp] = -1, 0, 1, 2 means indefinite, HP-INDEL, not-FD and not HP-INDEL, FD, respectively. + // Use majority rule to determine flow_disruptiveness_matrix of the family. + vector fd_type_counts(3); + for (unsigned int i_member = 0; i_member < all_family_members.size(); ++i_member){ + // Don't count outliers. + const CrossHypotheses* my_member = &(my_hypotheses[all_family_members[i_member]]); + if (my_member->OutlierByFlowDisruptiveness()){ + continue; + } + int fd_type = my_member->local_flow_disruptiveness_matrix[i_hyp][j_hyp]; + if (fd_type >= 0){ + fd_type_counts[fd_type] += my_member->read_counter; + } + } + int max_type = -1; + int max_count = 1; + for (unsigned int fd_type = 0; fd_type < 3; ++fd_type){ + // Claim higher FD level in the tie cases. + if (fd_type_counts[fd_type] >= max_count){ + max_type = fd_type; + max_count = fd_type_counts[fd_type]; + } + } + my_family_cross_.local_flow_disruptiveness_matrix[i_hyp][j_hyp] = max_type; + my_family_cross_.local_flow_disruptiveness_matrix[j_hyp][i_hyp] = max_type; + } + } +} + + diff --git a/Analysis/VariantCaller/EnsembleEval/CrossHypotheses.h b/Analysis/VariantCaller/EnsembleEval/CrossHypotheses.h index c6e3cd53..de5ef04d 100644 --- a/Analysis/VariantCaller/EnsembleEval/CrossHypotheses.h +++ b/Analysis/VariantCaller/EnsembleEval/CrossHypotheses.h @@ -27,12 +27,16 @@ class PrecomputeTDistOddN{ public: float v; + float log_v; float pi_factor; float v_factor; + float log_factor; int half_n; - PrecomputeTDistOddN(){v=pi_factor=v_factor=1.0f; half_n = 3; SetV(3);}; + PrecomputeTDistOddN(){v=pi_factor=v_factor=log_factor=log_v=1.0f; half_n = 3; SetV(3);}; void SetV(int _half_n); float TDistOddN(float res, float sigma, float skew); + float LogTDistOddN(float res, float sigma, float skew); + }; class HiddenBasis{ @@ -71,11 +75,12 @@ class CrossHypotheses{ vector normalized; // Normalized signal for test flows, it is the same for all hypotheses vector state_spread; vector same_as_null_hypothesis; // indicates whether a ref or alt hypothesis equals the read as called + vector measurement_var; // measurements var for a consensus read // keep the data at all flows here if preserve_full_data == true vector > predictions_all_flows; vector normalized_all_flows; - bool preserve_full_data = false; + vector measurement_sd_all_flows; HiddenBasis delta_state; bool use_correlated_likelihood; @@ -86,7 +91,7 @@ class CrossHypotheses{ vector > residuals; // difference prediction and observed vector > sigma_estimate; // estimate of variability per test flow per hypothesis for this read - vector > basic_likelihoods; // likelihood given residuals at each test flow of the observation at that flow != likelihood of read + vector > basic_log_likelihoods; // log-likelihood given residuals at each test flow of the observation at that flow != likelihood of read float skew_estimate; @@ -95,6 +100,7 @@ class CrossHypotheses{ // size number of hypotheses vector responsibility; // how responsible this read is for a given hypothesis under the MAP: size number of hypotheses (including null=outlier) + vector weighted_responsibility; // responsibility * read_counter_f vector log_likelihood; // sum over our test flows: logged to avoid under-flows vector scaled_likelihood; // actual sum likelihood over test flows, rescaled to null hypothesis (as called), derived from log_likelihood float ll_scale; // local scaling factor for scaled likelihood as can't trust null hypothesis to be near data @@ -103,12 +109,20 @@ class CrossHypotheses{ vector tmp_prob_f; vector tmp_prob_d; + // flow-disruptiveness for all pairs of hypotheses in the read level + // local_flow_disruptiveness_matrix[i][j] indicates the flow-disruptiveness between instance_of_read_by_state[i] and instance_of_read_by_state[j] + // -1: indefinite (e.g. no common suffix bases), 0: HP-INDEL (change of HP at only one flow), 1: Non-FD and not HP-INDEL (e.g., non-FD SNP), 2: FD + // @TODO: Use enumerate to represent the fd-code. + vector > local_flow_disruptiveness_matrix; + PrecomputeTDistOddN my_t; // useful hidden variables int strand_key; int heavy_tailed; + bool adjust_sigma; + float sigma_factor; int max_flows_to_test; float min_delta_for_flow; @@ -118,11 +132,18 @@ class CrossHypotheses{ int splice_start_flow; // Flow just before we start splicing in hypotheses (same for all hypotheses) int splice_end_flow; // Flow of the first base after the variant window (maximum over all hypotheses) int max_last_flow; // Last flow that is being simulated in prediction generation (max over all hypotheses) + + int read_counter; // Indicating how many reads form this read (>1 means it is a consensus read) + float read_counter_f; // float of read_counter bool success; + bool at_least_one_same_as_null; + // functions CrossHypotheses(){ heavy_tailed = 3; // t_5 degrees of freedom + adjust_sigma = false; + sigma_factor = 1.0f; my_t.SetV(3); strand_key = 0; max_last_flow=0; @@ -137,8 +158,13 @@ class CrossHypotheses{ magic_sigma_base = 0.085f; magic_sigma_slope = 0.0084f; use_correlated_likelihood = false; + read_counter = 1; + read_counter_f = 1.0f; + at_least_one_same_as_null = false; + local_flow_disruptiveness_matrix.clear(); }; void CleanAllocate(int num_hyp, int num_flow); + void ClearAllFlowsData(); void SetModPredictions(); void FillInPrediction(PersistingThreadObjects &thread_objects, const Alignment &my_read, const InputStructures &global_context); void InitializeDerivedQualities(); @@ -148,45 +174,52 @@ class CrossHypotheses{ void ResetModPredictions(); void ComputeDeltaCorrelation(); void ResetRelevantResiduals(); - void ComputeBasicLikelihoods(); + void ComputeBasicLogLikelihoods(); void ComputeLogLikelihoods(); void ComputeLogLikelihoodsSum(); void JointLogLikelihood(); void ComputeScaledLikelihood(); - float ComputePosteriorLikelihood(const vector &hyp_prob, float typical_prob); + float ComputePosteriorLikelihood(const vector &hyp_prob, float outlier_prob); void InitializeSigma(); void InitializeResponsibility(); - void UpdateResponsibility(const vector &hyp_prob, float typical_prob); + void UpdateResponsibility(const vector &hyp_prob, float outlier_prob); void UpdateRelevantLikelihoods(); void ComputeDelta(); bool ComputeAllComparisonsTestFlow(float threshold, int max_choice); float ComputeLLDifference(int a_hyp, int b_hyp); - int MostResponsible(); + int MostResponsible() const; bool IsValidTestFlowIndexOld(unsigned int flow,unsigned int max_choice); bool IsValidTestFlowIndexNew(unsigned int flow,unsigned int max_choice); - // HardOutlierClassifier is used to pre-filter out the outliers in a family. - // Then it is reasonable to say that a functional family contains no outliers. - bool LocalOutlierClassifier(float typical_prob); + void FillInFlowDisruptivenessMatrix(const ion::FlowOrder &flow_order, const Alignment &my_alignment); + bool OutlierByFlowDisruptiveness() const; }; // Deal with the inference for a single family -class EvalFamily : public MolecularFamily{ +class EvalFamily : public AbstractMolecularFamily{ public: vector family_responsibility; - EvalFamily(const string &barcode, int strand): MolecularFamily(barcode, strand) {}; + EvalFamily(const string &barcode, int strand, const vector* const read_stack) + : AbstractMolecularFamily(barcode, strand), read_stack_(read_stack) {}; + ~EvalFamily(){}; + int CountFamSizeFromValid(); + int CountFamSizeFromAll(); void InitializeEvalFamily(unsigned int num_hyp); void CleanAllocate(unsigned int num_hyp); void InitializeFamilyResponsibility(); void ComputeFamilyLogLikelihoods(const vector &my_hypotheses); - void UpdateFamilyResponsibility(const vector &hyp_prob, float typical_prob); - float ComputeFamilyPosteriorLikelihood(const vector &hyp_prob, float typical_prob); + void UpdateFamilyResponsibility(const vector &hyp_prob, float outlier_prob); + void ComputeFamilyOutlierResponsibility(const vector &my_hypotheses, unsigned int min_fam_size); + float ComputeFamilyPosteriorLikelihood(const vector &hyp_prob); + float ComputeLLDifference(int a_hyp, int b_hyp) {return my_family_cross_.ComputeLLDifference(a_hyp, b_hyp);}; int MostResponsible(); vector GetFamilyLogLikelihood() const{ return my_family_cross_.log_likelihood; }; vector GetFamilyScaledLikelihood() const{ return my_family_cross_.scaled_likelihood; }; - + void FillInFlowDisruptivenessMatrix(const vector &my_hypotheses); + int GetFlowDisruptiveness(int i_hyp, int j_hyp) const { return my_family_cross_.local_flow_disruptiveness_matrix[i_hyp][j_hyp]; }; private: + const vector* const read_stack_; // Used to calculate family size // The calculation of log-likelihood etc. of a family is pretty much the same as a single read. // my_family_cross_ is used for calculating the "likelihoods" and "responsibility" only. // must be use my_family_cross_ carefully since it has a lot of uninitialized members. @@ -194,4 +227,6 @@ class EvalFamily : public MolecularFamily{ CrossHypotheses my_family_cross_; }; +bool IsHpIndel(const string& seq_1, const string& seq_2); + #endif // CROSSHYPOTHESES_H diff --git a/Analysis/VariantCaller/EnsembleEval/DiagnosticJSON.cpp b/Analysis/VariantCaller/EnsembleEval/DiagnosticJSON.cpp index c0a03db3..5bf0cd40 100644 --- a/Analysis/VariantCaller/EnsembleEval/DiagnosticJSON.cpp +++ b/Analysis/VariantCaller/EnsembleEval/DiagnosticJSON.cpp @@ -68,6 +68,7 @@ void DiagnosticJsonCrossHypotheses(Json::Value &json, const CrossHypotheses &my_ json["strand"] = my_cross.strand_key; json["success"] = my_cross.success ? 1 : 0; json["usecorr"] = my_cross.use_correlated_likelihood ? 1: 0; + json["read_counter"] = my_cross.read_counter; json["heavy"] = my_cross.heavy_tailed; json["lastrelevantflow"] = my_cross.max_last_flow; @@ -100,7 +101,7 @@ void DiagnosticJsonCrossHypotheses(Json::Value &json, const CrossHypotheses &my_ my_cross_temp.mod_predictions[i_hyp][j_flow] = my_cross.mod_predictions[i_hyp][t_flow]; my_cross_temp.residuals[i_hyp][j_flow] = my_cross.residuals[i_hyp][t_flow]; my_cross_temp.sigma_estimate[i_hyp][j_flow] = my_cross.sigma_estimate[i_hyp][t_flow]; - my_cross_temp.basic_likelihoods[i_hyp][j_flow] = my_cross.basic_likelihoods[i_hyp][t_flow]; + my_cross_temp.basic_log_likelihoods[i_hyp][j_flow] = my_cross.basic_log_likelihoods[i_hyp][t_flow]; } } @@ -120,17 +121,23 @@ void DiagnosticJsonCrossHypotheses(Json::Value &json, const CrossHypotheses &my_ json["testflows"][i_test] = my_cross.test_flow[i_test]; // hold some intermediates size data matrix hyp * nFlows (should be active flows) - - for (unsigned int i_hyp = 0; i_hyp < my_cross_temp.predictions.size(); i_hyp++) { - for (unsigned int i_flow = 0; i_flow < my_cross_temp.predictions[0].size(); i_flow++) { + for (unsigned int i_flow = 0; i_flow < my_cross_temp.predictions[0].size(); i_flow++) { + for (unsigned int i_hyp = 0; i_hyp < my_cross_temp.predictions.size(); i_hyp++) { json["predictions"][i_hyp][i_flow] = my_cross_temp.predictions[i_hyp][i_flow]; json["modpred"][i_hyp][i_flow] = my_cross_temp.mod_predictions[i_hyp][i_flow]; json["normalized"][i_hyp][i_flow] = my_cross_temp.normalized[i_flow]; json["residuals"][i_hyp][i_flow] = my_cross_temp.residuals[i_hyp][i_flow]; json["sigma"][i_hyp][i_flow] = my_cross_temp.sigma_estimate[i_hyp][i_flow]; - json["basiclikelihoods"][i_hyp][i_flow] = my_cross_temp.basic_likelihoods[i_hyp][i_flow]; + json["basiclikelihoods"][i_hyp][i_flow] = exp(my_cross_temp.basic_log_likelihoods[i_hyp][i_flow]); // For historical reasons, dump the likelihood w/o taking logarithm } } + + if (not my_cross_temp.measurement_var.empty()){ + for (unsigned int i_flow = 0; i_flow < my_cross_temp.measurement_var.size(); i_flow++) + json["measurement_var"][i_flow] = my_cross_temp.measurement_var[i_flow]; + } + + // sequence, responsibility (after clustering), etc for (unsigned int i_hyp = 0; i_hyp < my_cross.responsibility.size(); i_hyp++) { json["instancebystate"][i_hyp] = my_cross.instance_of_read_by_state[i_hyp]; @@ -143,6 +150,7 @@ void DiagnosticJsonCrossHypotheses(Json::Value &json, const CrossHypotheses &my_ void TinyDiagnosticJsonCrossHypotheses(Json::Value &json, const CrossHypotheses &my_cross) { json["strand"] = my_cross.strand_key; + json["read_counter"] = my_cross.read_counter; json["success"] = my_cross.success ? 1 : 0; // active flows over which we are testing @@ -161,14 +169,14 @@ json["strand"] = my_cross.strand_key; void DiagnosticJsonEvalFamily(Json::Value &json, const EvalFamily &my_family) { json["family_barcaode"] = my_family.family_barcode; json["strand_key"] = my_family.strand_key; - json["is_func_family"] = my_family.GetFunctionality() ? 1 : 0; + json["is_func_family"] = my_family.GetFuncFromValid() ? 1 : 0; // members in the family - for (unsigned int i_ndx = 0; i_ndx < my_family.family_members.size(); i_ndx++) { - json["family_members"][i_ndx] = my_family.family_members[i_ndx]; + for (unsigned int i_ndx = 0; i_ndx < my_family.valid_family_members.size(); i_ndx++) { + json["valid_family_members"][i_ndx] = my_family.valid_family_members[i_ndx]; } - if(my_family.GetFunctionality()){ + if(my_family.GetFuncFromValid()){ vector family_log_likelihood(my_family.GetFamilyLogLikelihood()); vector family_scaled_likelihood(my_family.GetFamilyScaledLikelihood()); // responsibility (after clustering), etc @@ -185,11 +193,11 @@ void DiagnosticJsonEvalFamily(Json::Value &json, const EvalFamily &my_family) { void TinyDiagnosticJsonEvalFamily(Json::Value &json, const EvalFamily &my_family) { json["family_barcaode"] = my_family.family_barcode; json["strand_key"] = my_family.strand_key; - json["is_func_family"] = my_family.GetFunctionality() ? 1 : 0; + json["is_func_family"] = my_family.GetFuncFromValid() ? 1 : 0; // members in the family - for (unsigned int i_ndx = 0; i_ndx < my_family.family_members.size(); i_ndx++) { - json["family_members"][i_ndx] = my_family.family_members[i_ndx]; + for (unsigned int i_ndx = 0; i_ndx < my_family.valid_family_members.size(); i_ndx++) { + json["family_members"][i_ndx] = my_family.valid_family_members[i_ndx]; } } diff --git a/Analysis/VariantCaller/EnsembleEval/PosteriorInference.cpp b/Analysis/VariantCaller/EnsembleEval/PosteriorInference.cpp index 9f289d73..0f0b0004 100644 --- a/Analysis/VariantCaller/EnsembleEval/PosteriorInference.cpp +++ b/Analysis/VariantCaller/EnsembleEval/PosteriorInference.cpp @@ -3,7 +3,8 @@ #include "PosteriorInference.h" ScanSpace::ScanSpace(){ - scan_done = false; + scan_pair_done = false; + scan_ref_done = false; freq_pair.assign(2,0); // reference and hypothesis freq_pair[1]=1; @@ -12,21 +13,19 @@ ScanSpace::ScanSpace(){ max_ll = -999999999.0f; // anything is better than this max_index = 0; min_detail_level_for_fast_scan = 2500; - // member variables for fast scan - + max_detail_level = 0; + // private variables for fast scan is_scanned_.clear(); - coarse_freq_resolution_ = 0.01f; - num_of_fibonacci_blocks = 3; // Assume the maximums for Hom, Het, Hom - fine_log_posterior_cutoff_gap_ = -log(0.00001f); - fine_freq_search_increment_ = 0.02f; - min_fine_log_posterior_gap_ = 0.01f; - fine_scan_penalty_order_ = 1.5f; max_log_posterior_scanned_ = -999999999999.0f; // anything is better than this argmax_log_posterior_scanned_ = 0; + + scan_more_frequencies_ = {0.0001f, 0.0005f, 0.001f, 0.005f}; // prevent singularity at edge frequencies + + DEBUG = 0; } FreqMaster::FreqMaster(){ - data_reliability = 1.0f-0.001f; + outlier_prob = 0.001f; max_hyp_freq.resize(2); prior_frequency_weight.resize(2); prior_frequency_weight.assign(2,0.0f); // additional prior weight here @@ -45,12 +44,11 @@ bool FreqMaster::Compare(vector &original, int numreads, float threshold } PosteriorInference::PosteriorInference() { - params_ll = 0.0f; - vector local_freq_start; local_freq_start.assign(2,0.5f); clustering.SetHypFreq(local_freq_start); + DEBUG = 0; } void FreqMaster::SetHypFreq(const vector &local_freq){ @@ -181,7 +179,7 @@ float ScanSpace::LogDefiniteIntegral(float _alpha, float _beta) { float beta_interpolate = exp(log_posterior_by_frequency[beta_low] - local_max_ll) * (1.0 - delta_beta) + exp(log_posterior_by_frequency[beta_hi] - local_max_ll) * delta_beta; // trapezoidal rule - float integral_sum = 0.0f; + double integral_sum = 0.0; // use double to calculate cumsum to enhance numerical stability. float distance_to_next; float cur_point_val; float cur_area; @@ -193,24 +191,24 @@ float ScanSpace::LogDefiniteIntegral(float _alpha, float _beta) { cur_point_val = exp(log_posterior_by_frequency[alpha_hi] - local_max_ll); cur_area = 0.5f * distance_to_next * (alpha_interpolate + cur_point_val); old_point_val = cur_point_val; - integral_sum += cur_area; // distance to starting integer + integral_sum += (double) cur_area; // distance to starting integer // interior segments for (int i_eval = alpha_hi + 1; i_eval <= beta_low; i_eval++) { cur_point_val = exp(log_posterior_by_frequency[i_eval] - local_max_ll); - cur_area = 0.5 * (old_point_val + cur_point_val); - integral_sum += cur_area; + cur_area = 0.5f * (old_point_val + cur_point_val); + integral_sum += (double) cur_area; old_point_val = cur_point_val; } // final segments distance_to_next = beta_n - beta_low; cur_area = 0.5f * distance_to_next * (old_point_val + beta_interpolate); - integral_sum += cur_area; + integral_sum += (double) cur_area; } else { - integral_sum = 0.5f * (beta_n - alpha_n) * (alpha_interpolate + beta_interpolate); + integral_sum = (double) (0.5f * (beta_n - alpha_n) * (alpha_interpolate + beta_interpolate)); } // now rescale back on log-scale - float integral_log = log(integral_sum) + local_max_ll; + float integral_log = log((float) integral_sum) + local_max_ll; if (isnan(integral_log)) { // trap! @@ -250,16 +248,18 @@ void FibInterval(vector &samples, int eval_start, int detail_level std::sort(samples.begin(), samples.end()); } -unsigned int ScanSpace::ResizeToMatch(ShortStack &total_theory, unsigned max_detail_level ){ - unsigned int detail_level = total_theory.DetailLevel(); - if(max_detail_level>0) detail_level = (detail_level < max_detail_level) ? (detail_level+1) : (max_detail_level+1); - float fdetail_level = (float) detail_level; +unsigned int ScanSpace::ResizeToMatch(ShortStack &total_theory, unsigned int max_detail_level){ + unsigned int detail_level = total_theory.DetailLevel(); + if (max_detail_level > 0 and detail_level > max_detail_level){ + detail_level = max_detail_level; + } + float fdetail_level = (float) detail_level; log_posterior_by_frequency.resize(detail_level + 1); eval_at_frequency.resize(detail_level + 1); for (unsigned int i_eval = 0; i_eval < eval_at_frequency.size(); i_eval++) { - eval_at_frequency[i_eval] = (float)i_eval / fdetail_level; - } - return(detail_level); + eval_at_frequency[i_eval] = (float) i_eval / fdetail_level; + } + return detail_level; } @@ -309,47 +309,96 @@ void PosteriorInference::InterpolateFrequencyScan(ShortStack &total_theory, bool scan_done = true; }*/ -void ScanSpace::DoPosteriorFrequencyScan(ShortStack &total_theory, FreqMaster &base_clustering, bool update_frequency, int strand_key, bool scan_ref, int max_detail_level) { +void ScanSpace::DoFullScan_(){ + if(DEBUG > 1){ + cout << " - Do full scan: scan all " << eval_at_frequency.size() << " frequencies."<< endl; + } + max_log_posterior_scanned_ = -999999999999.0f; // anything is better than this + argmax_log_posterior_scanned_ = 0; + + for (unsigned int i_eval = 0; i_eval < eval_at_frequency.size(); ++i_eval){ + DoPosteriorFrequencyScanOneHypFreq_(i_eval); + UpdateMaxPosteior_(i_eval); + } + max_ll = max_log_posterior_scanned_; + max_index = (int) argmax_log_posterior_scanned_; +} + +void ScanSpace::DoPosteriorFrequencyScan(ShortStack &total_theory, FreqMaster &base_clustering, bool update_frequency, int strand_key, bool scan_ref) { // cout << "ScanningFrequency" << endl; // posterior frequency inference given current data/likelihood pairing - unsigned int detail_level = ResizeToMatch(total_theory, (unsigned) max_detail_level); // now fills in frequency + unsigned long t0 = clock(); + unsigned int detail_level = ResizeToMatch(total_theory, max_detail_level); // now fills in frequency - is_scanned_.clear(); // Very important step! + is_scanned_.resize(0); // (Important): Always make sure is_scanned_ is empty. // Set the pointers for DoPosteriorFrequencyScanOneHypFreq_ ptr_total_theory_ = &total_theory; ptr_base_clustering_ = &base_clustering; ptr_strand_key_ = &strand_key; ptr_scan_ref_ = &scan_ref; - if(detail_level < min_detail_level_for_fast_scan or detail_level < (unsigned int)(1.0f / coarse_freq_resolution_)){ - // Do full scan - for (unsigned int i_eval = 0; i_eval < eval_at_frequency.size(); ++i_eval){ - DoPosteriorFrequencyScanOneHypFreq_(i_eval); + if (DEBUG > 1){ + cout << " + Do posterior frequency scan:" << endl; + cout << " - Baseline allele_freq = " << PrintIteratorToString(base_clustering.max_hyp_freq.begin(), base_clustering.max_hyp_freq.end()) << endl; + if (scan_ref) { + cout << " - Scan type: ref vs. (all alt) => f = allele_freq[0]/allele_freq[1:]" << endl; + } + else { + cout << " - Scan type: (allele "<< freq_pair[0] <<") vs. (allele "<< freq_pair[1] + << ") => f = allele_freq[" << freq_pair[0] << "]/(allele_freq[" << freq_pair[0] << "]+allele_freq[" << freq_pair[1] << "])" << endl; } - FindMaxFrequency(); + } + + if(detail_level < min_detail_level_for_fast_scan or detail_level < (unsigned int)(1.0f / kCoarseFreqResolution_)){ + DoFullScan_(); } else{ DoFastScan_(); - max_ll = max_log_posterior_scanned_; - max_index = (int) argmax_log_posterior_scanned_; } - // if doing monomorphic eval, set frequency to begin with and don't update //FindMaxFrequency(update_frequency); // log_posterior now contains all frequency information inferred from the data - scan_done = true; + if(scan_ref){ + scan_ref_done = true; + }else{ + scan_pair_done = true; + } // clear the pointers for DoPosteriorFrequencyScanOneHypFreq_ ptr_total_theory_ = NULL; ptr_base_clustering_ = NULL; ptr_strand_key_ = NULL; ptr_scan_ref_ = NULL; + + if (DEBUG > 1){ + cout << " + Posterior frequency scan done. Processing time = " << (double) (clock() - t0) / 1E6 << " sec." << endl; + float f_resolution_debug = 0.05f; + unsigned int num_freq_debug = (unsigned int) (1.0f / f_resolution_debug) + 1; + if (num_freq_debug >= log_posterior_by_frequency.size()){ + cout << " - Scan results: (f, log-posterior(f)) = "; + for (unsigned int i_eval = 0; i_eval < log_posterior_by_frequency.size(); ++i_eval){ + cout << "(" << eval_at_frequency[i_eval] << ", " << log_posterior_by_frequency[i_eval] << "), "; + } + } + else{ + cout << " - Sampled scan results: (f, log-posterior(f)) = "; + for(unsigned int i = 0; i < num_freq_debug; ++i){ + unsigned int i_eval = (i == (num_freq_debug - 1))? eval_at_frequency.size() - 1: (unsigned int) (double(i * (log_posterior_by_frequency.size() - 1)) / double(num_freq_debug - 1)); + cout << "(" << eval_at_frequency[i_eval] << ", " << log_posterior_by_frequency[i_eval] << "), "; + } + } + cout << endl; + cout << " - max(log-posterior(f)) = " << max_ll << " @ f = "<< eval_at_frequency[max_index] << endl; + } } void ScanSpace::DoPosteriorFrequencyScanOneHypFreq_(unsigned int i_eval){ if(not is_scanned_.empty()){ - if(is_scanned_[i_eval]) + if(is_scanned_[i_eval]){ return; // scan has been done at i_eval + } + // I am going to scan at i_eval. + is_scanned_[i_eval] = true; } vector hyp_freq = ptr_base_clustering_->max_hyp_freq; @@ -360,12 +409,7 @@ void ScanSpace::DoPosteriorFrequencyScanOneHypFreq_(unsigned int i_eval){ else{ ptr_base_clustering_->UpdateFrequencyAgainstOne(hyp_freq, eval_at_frequency[i_eval], 0); } - log_posterior_by_frequency[i_eval] = ptr_total_theory_->PosteriorFrequencyLogLikelihood(hyp_freq, ptr_base_clustering_->prior_frequency_weight, ptr_base_clustering_->germline_log_prior_normalization, ptr_base_clustering_->data_reliability, *ptr_strand_key_); - - if(not is_scanned_.empty()){ - // mark as scanned - is_scanned_[i_eval] = true; - } + log_posterior_by_frequency[i_eval] = ptr_total_theory_->PosteriorFrequencyLogLikelihood(hyp_freq, ptr_base_clustering_->prior_frequency_weight, ptr_base_clustering_->germline_log_prior_normalization, ptr_base_clustering_->outlier_prob, *ptr_strand_key_); } @@ -374,8 +418,8 @@ void PosteriorInference::UpdateMaxFreqFromResponsibility(ShortStack &total_theor // skip time consuming scan and use responsibilities as cluster entry total_theory.MultiFrequencyFromResponsibility(clustering.max_hyp_freq, clustering.prior_frequency_weight, strand_key); - ref_vs_all.max_ll = total_theory.PosteriorFrequencyLogLikelihood(clustering.max_hyp_freq, clustering.prior_frequency_weight,clustering.germline_log_prior_normalization, clustering.data_reliability, strand_key); - ref_vs_all.scan_done = false; // didn't come from scan + ref_vs_all.max_ll = total_theory.PosteriorFrequencyLogLikelihood(clustering.max_hyp_freq, clustering.prior_frequency_weight,clustering.germline_log_prior_normalization, clustering.outlier_prob, strand_key); + ref_vs_all.scan_ref_done = false; // didn't come from scan } @@ -396,15 +440,21 @@ void PosteriorInference::StartAtHardClassify(ShortStack &total_theory, bool upda if (update_frequency) { clustering.SetHypFreq(start_frequency); clustering.SetPriorStrength(start_frequency); - ref_vs_all.max_ll = total_theory.PosteriorFrequencyLogLikelihood(clustering.max_hyp_freq, clustering.prior_frequency_weight,clustering.germline_log_prior_normalization, clustering.data_reliability, ALL_STRAND_KEY); + ref_vs_all.max_ll = total_theory.PosteriorFrequencyLogLikelihood(clustering.max_hyp_freq, clustering.prior_frequency_weight,clustering.germline_log_prior_normalization, clustering.outlier_prob, ALL_STRAND_KEY); } - total_theory.UpdateResponsibility(clustering.max_hyp_freq, clustering.data_reliability); + total_theory.UpdateResponsibility(clustering.max_hyp_freq, clustering.outlier_prob); } void PosteriorInference::QuickUpdateStep(ShortStack &total_theory){ UpdateMaxFreqFromResponsibility(total_theory, ALL_STRAND_KEY); - total_theory.UpdateResponsibility(clustering.max_hyp_freq, clustering.data_reliability); // update cluster responsibilities + total_theory.UpdateResponsibility(clustering.max_hyp_freq, clustering.outlier_prob); // update cluster responsibilities + + if(DEBUG > 1){ + cout << " + allele_freq updated from responsibility "<< endl + << " - allele_freq = " << PrintIteratorToString(clustering.max_hyp_freq.begin(), clustering.max_hyp_freq.end()) << endl + << " - ref_vs_all.max_ll = " << ReturnJustLL() << endl; + } } /* void PosteriorInference::DetailedUpdateStep(ShortStack &total_theory, bool update_frequency){ @@ -412,39 +462,28 @@ void PosteriorInference::DetailedUpdateStep(ShortStack &total_theory, bool updat total_theory.UpdateResponsibility(max_hyp_freq, data_reliability); // update cluster responsibilities }*/ - -// functions for fast scan - -// Note that i_1 can't equal i_2 -void ScanSpace::LinearInterpolation_(unsigned int i_1, unsigned int i_2, unsigned int i_intp){ - if(i_intp == i_1){ - log_posterior_by_frequency[i_intp] = log_posterior_by_frequency[i_1]; - return; - } - if(i_intp == i_2){ - log_posterior_by_frequency[i_intp] = log_posterior_by_frequency[i_2]; - return; - } - log_posterior_by_frequency[i_intp] = log_posterior_by_frequency[i_1] + (log_posterior_by_frequency[i_2] - log_posterior_by_frequency[i_1]) * float(i_intp - i_1) / float(i_2 - i_1); -} - void ScanSpace::DoInterpolation_(){ unsigned int scanned_idx_left = 0; unsigned int scanned_idx_right = 0; - // make sure we scan the first and last + // Always make sure we scan the first and last DoPosteriorFrequencyScanOneHypFreq_(0); DoPosteriorFrequencyScanOneHypFreq_(eval_at_frequency.size() - 1); - for(unsigned int i_eval = 0; i_eval < eval_at_frequency.size(); ++i_eval){ - if(is_scanned_[i_eval]){ - scanned_idx_left = i_eval; - continue; - } - while(scanned_idx_right <= i_eval or (not is_scanned_[scanned_idx_right])){ + while(scanned_idx_left < eval_at_frequency.size() - 1){ + scanned_idx_right = scanned_idx_left + 1; + while(not is_scanned_[scanned_idx_right]){ ++scanned_idx_right; } - LinearInterpolation_(scanned_idx_right, scanned_idx_left, i_eval); + float delta_idx = (float) (scanned_idx_right - scanned_idx_left); + for (unsigned int i_eval = scanned_idx_left + 1; i_eval < scanned_idx_right; ++i_eval){ + float alpha = (float) (i_eval - scanned_idx_left) / delta_idx; + // Do linear interpolation here. + // My experiments show that doing interpolation in log-domain has better result. + // Although quadratic interpolation (i.e., assume posterior is Gaussian) has lower interpolation error, the linear interpolation is good enough. + log_posterior_by_frequency[i_eval] = alpha * log_posterior_by_frequency[scanned_idx_right] + (1.0f - alpha) * log_posterior_by_frequency[scanned_idx_left]; + } + scanned_idx_left = scanned_idx_right; } } @@ -513,21 +552,23 @@ void ScanSpace::DoFineScan_(unsigned int i_left, unsigned int i_right, unsigned DoPosteriorFrequencyScanOneHypFreq_(i_left); DoPosteriorFrequencyScanOneHypFreq_(i_right); DoPosteriorFrequencyScanOneHypFreq_(i_middle); + UpdateMaxPosteior_(i_left); + UpdateMaxPosteior_(i_right); + UpdateMaxPosteior_(i_middle); left_max = max(log_posterior_by_frequency[i_left], log_posterior_by_frequency[i_middle]); right_max = max(log_posterior_by_frequency[i_right], log_posterior_by_frequency[i_middle]); - max_log_posterior_scanned_ = max(max_log_posterior_scanned_, max(left_max, right_max)); if(i_middle - i_left > 1){ // The penalty is used to obtain finer resolution around max_log_posterior_scanned_ - penalty = exp(- fine_scan_penalty_order_ * (max_log_posterior_scanned_ - left_max)); // 0 < penalty <= 1 + penalty = exp(- kFineScanPenaltyOrder_ * (max_log_posterior_scanned_ - left_max)); // 0 < penalty <= 1 // if (the gap between the two points) * penalty > min_fine_log_posterior_gap_, we scan the middle of the two points - if (abs(log_posterior_by_frequency[i_middle] - log_posterior_by_frequency[i_left]) * penalty > min_fine_log_posterior_gap_) + if (abs(log_posterior_by_frequency[i_middle] - log_posterior_by_frequency[i_left]) * penalty > kMinFineLogPosteriorGap_) DoFineScan_(i_left, i_middle, (i_left + i_middle) / 2); } if(i_right - i_middle > 1){ - penalty = exp(- fine_scan_penalty_order_ * (max_log_posterior_scanned_ - right_max)); - if (abs(log_posterior_by_frequency[i_middle] - log_posterior_by_frequency[i_right]) * penalty > min_fine_log_posterior_gap_) + penalty = exp(- kFineScanPenaltyOrder_ * (max_log_posterior_scanned_ - right_max)); + if (abs(log_posterior_by_frequency[i_middle] - log_posterior_by_frequency[i_right]) * penalty > kMinFineLogPosteriorGap_) DoFineScan_(i_middle, i_right, (i_right + i_middle) / 2); } @@ -542,31 +583,42 @@ void ScanSpace::DoFineScan_(unsigned int i_left, unsigned int i_right, unsigned // (a. For the EM algorithm) The maximum of log_posterior_by_frequency // (b. For PASS/NOCALL, QUAL, GT, GQ) The definite integral of posterior_by_frequency (i.e., convert log_posterior_by_frequency to linear scale and normalize it) // DoFastScan_() computes an approximate log_posterior_by_frequency that provides good results for both (a) and (b) without scanning all frequencies. +// I will scan three neighborhood of frequencies: +// (a): The neighborhood around the peak of posterior_by_frequency +// (b): The neighborhoods around min_allele_freq and 1 - min_allele_freq +// (c): The neighborhoods around f = 0.0 and f = 1.0 (In case singularity in the edge cases) // In particular, the number of frequencies scanned = O(log(N)), which results the complexity of DoFastScan_() = O(N*log(N)). // (Note 1): The underlying assumption of log_posterior_by_frequency for DoFastScan_() is that log_posterior_by_frequency is "unimodal" (at least in large scale). // (Note 2): DoFastScan_() is a "blind" scan algorithm that has no information about log_posterior_by_frequency priorly. -// @TODO: Instead of the "blind" approach, the fast scan algorithm can be improved by taking max_hyp_freq into account. +// (Note 3): The interpolation error should be neglectable in liner scale, while the error may show up in the log-domain (i.e., in "large" QUAL). void ScanSpace::DoFastScan_(){ unsigned int num_scanned_freq = 0; // Step 0): Initialization + unsigned int num_coarse_scan = (unsigned int) (1.0f / kCoarseFreqResolution_) + 1; // also scan the last index + // Is it necessarily to do fast scan? + if( 0.9f * (float) eval_at_frequency.size() <= (float) num_coarse_scan){ + DoFullScan_(); + return; + } + unsigned int detail_level = eval_at_frequency.size() - 1; is_scanned_.assign(detail_level + 1, false); max_log_posterior_scanned_ = -999999999999.0f; // anything is better than this argmax_log_posterior_scanned_ = 0; - if(debug_){ - cout<<"<>"<< endl; - cout<<" detail_level = "<< detail_level<< endl; - cout<<" # of reads = "<my_hypotheses.size()< 1){ + if(ptr_total_theory_->GetIsMolecularTag()){ + cout << " - Do fast scan (detail_level = "<< detail_level << ", number of functional families = "<< ptr_total_theory_->GetNumFuncFamilies() << ")" << endl; + } + else{ + cout << " - Do fast scan (detail_level = "<< detail_level << ", number of valid reads = "<< ptr_total_theory_->valid_indexes.size() << ")" << endl; + } } - // Step 1): Do coarse scan float croase_max_log_posterior = 0.0f; unsigned int croase_argmax_log_posterior = 0; - unsigned int num_coarse_scan = (unsigned int) (1.0f / coarse_freq_resolution_) + 1; // also scan the last index - unsigned int coarse_scan_spacing = detail_level / (num_coarse_scan - 1); vector coarse_scan_indices; coarse_scan_indices.assign(num_coarse_scan, 0); @@ -577,7 +629,7 @@ void ScanSpace::DoFastScan_(){ croase_argmax_log_posterior = coarse_scan_indices.size() - 1; for(unsigned int i = 0; i < num_coarse_scan - 1; ++i){ - unsigned int i_eval = i * coarse_scan_spacing; + unsigned int i_eval = (unsigned int) (double(i * (detail_level)) / double(num_coarse_scan - 1)); coarse_scan_indices[i] = i_eval; DoPosteriorFrequencyScanOneHypFreq_(i_eval); if(log_posterior_by_frequency[i_eval] > croase_max_log_posterior){ @@ -586,26 +638,13 @@ void ScanSpace::DoFastScan_(){ } } - if(debug_){ - unsigned int num_scanned_freq_tmp = 0; - for(unsigned int i_eval = 0; i_eval < is_scanned_.size(); ++i_eval){ - num_scanned_freq_tmp += (unsigned int) is_scanned_[i_eval]; - } - cout<<" # of coarse freq scanned = "<< num_scanned_freq_tmp - num_scanned_freq<<" (coarse_freq_resolution_= "<< coarse_freq_resolution_<< ")"< max_log_posterior_scanned_){ - argmax_log_posterior_scanned_ = i_eval; - max_log_posterior_scanned_ = log_posterior_by_frequency[argmax_log_posterior_scanned_]; - } + UpdateMaxPosteior_(i_eval); } // In case Fibonacci search returns a local maximum which is less than croase_max_log_posterior if(max_log_posterior_scanned_ < croase_max_log_posterior){ @@ -613,18 +652,34 @@ void ScanSpace::DoFastScan_(){ unsigned int i_left = croase_argmax_log_posterior == 0? coarse_scan_indices[0] : coarse_scan_indices[croase_argmax_log_posterior - 1]; unsigned int i_right = croase_argmax_log_posterior == coarse_scan_indices.size() - 1? coarse_scan_indices[coarse_scan_indices.size() - 1] : coarse_scan_indices[croase_argmax_log_posterior + 1]; unsigned int i_eval = FibonacciSearchMax_(i_left, i_right); - if(log_posterior_by_frequency[i_eval] < croase_max_log_posterior){ - // croase_max_log_posterior still beats the search results, although it shouldn't happen. - max_log_posterior_scanned_ = croase_max_log_posterior; - argmax_log_posterior_scanned_ = coarse_scan_indices[croase_argmax_log_posterior]; - } - else{ - argmax_log_posterior_scanned_ = i_eval; - max_log_posterior_scanned_ = log_posterior_by_frequency[argmax_log_posterior_scanned_]; - } + UpdateMaxPosteior_(croase_argmax_log_posterior); // in case croase_max_log_posterior still beats the search results, although it shouldn't happen. + UpdateMaxPosteior_(i_eval); } - // Step 3): Determine the interval for fine scan + if(DEBUG > 1){ + cout << " - Fibonacci search found max(log-posterior(f)) = "<< max_log_posterior_scanned_<< " @ f = eval_at_frequency["<< argmax_log_posterior_scanned_ << "] = " << eval_at_frequency[argmax_log_posterior_scanned_] << endl; + } + + // Step 3): + // Scan the frequencies at scan_more_frequencies to enhance the accuracy. + // The entries in scan_more_frequencies are obtained from: a) edge frequencies, b) min-allele-freq + for (unsigned int i_freq = 0; i_freq < scan_more_frequencies_.size(); ++i_freq){ + unsigned int i_eval = (unsigned int) ( (double) detail_level * (double) scan_more_frequencies_[i_freq]); + DoPosteriorFrequencyScanOneHypFreq_(i_eval); // scan freq + UpdateMaxPosteior_(i_eval); + DoPosteriorFrequencyScanOneHypFreq_(detail_level - i_eval); // scan 1.0 - freq + UpdateMaxPosteior_(detail_level - i_eval); + } + // Scan more edge frequencies to prevent singularity + unsigned int scan_more_edge_index = (eval_at_frequency.size() < 8)? eval_at_frequency.size() : 8; + for (unsigned int i_eval = 0; i_eval < scan_more_edge_index; ++i_eval){ + DoPosteriorFrequencyScanOneHypFreq_(i_eval); + UpdateMaxPosteior_(i_eval); + DoPosteriorFrequencyScanOneHypFreq_(detail_level - i_eval); + UpdateMaxPosteior_(detail_level - i_eval); + } + + // Step 4): Determine the interval for fine scan // For (b. For PASS/NOCALL, QUAL, GT, GQ) The definite integral of posterior_by_frequency (i.e., convert log_posterior_by_frequency to linear scale and normalize it)), // what I really care about is the main mass of the posterior probability function. // The log-posterior(f) that is dominated by max_log_posterior_scanned_ is not important at all. @@ -632,15 +687,12 @@ void ScanSpace::DoFastScan_(){ // I start from the frequency at max_log_posterior_scanned_, and then increase/decrease by fine_freq_search_increment_ until I obtain // log_posterior(f) - max_log_posterior_scanned_ > fine_log_posterior_cutoff_gap_. // Then I do fine scan within the interval [fine_cutoff_i_left, fine_cutoff_i_right] - int index_increament = max((int)((float) detail_level * fine_freq_search_increment_), 1); + int index_increament = max((int)((float) detail_level * kFineFreqSearchIncrement_), 1); int fine_cutoff_i_left = (int) argmax_log_posterior_scanned_; while(fine_cutoff_i_left >= 0){ DoPosteriorFrequencyScanOneHypFreq_((unsigned int) fine_cutoff_i_left); - if(log_posterior_by_frequency[fine_cutoff_i_left] > max_log_posterior_scanned_){ - argmax_log_posterior_scanned_ = (unsigned int) fine_cutoff_i_left; - max_log_posterior_scanned_ = log_posterior_by_frequency[argmax_log_posterior_scanned_]; - } - if(max_log_posterior_scanned_ - log_posterior_by_frequency[fine_cutoff_i_left] > fine_log_posterior_cutoff_gap_) + UpdateMaxPosteior_((unsigned int) fine_cutoff_i_left); + if(max_log_posterior_scanned_ - log_posterior_by_frequency[fine_cutoff_i_left] > kFineLogPosteriorCutoffGap_) break; fine_cutoff_i_left -= index_increament; @@ -650,56 +702,100 @@ void ScanSpace::DoFastScan_(){ unsigned int fine_cutoff_i_right = argmax_log_posterior_scanned_; while(fine_cutoff_i_right <= detail_level){ DoPosteriorFrequencyScanOneHypFreq_(fine_cutoff_i_right); - if(log_posterior_by_frequency[fine_cutoff_i_right] > max_log_posterior_scanned_){ - argmax_log_posterior_scanned_ = fine_cutoff_i_right; - max_log_posterior_scanned_ = log_posterior_by_frequency[argmax_log_posterior_scanned_]; - } - if(max_log_posterior_scanned_ - log_posterior_by_frequency[fine_cutoff_i_right] > fine_log_posterior_cutoff_gap_) + UpdateMaxPosteior_((unsigned int) fine_cutoff_i_right); + if(max_log_posterior_scanned_ - log_posterior_by_frequency[fine_cutoff_i_right] > kFineLogPosteriorCutoffGap_) break; fine_cutoff_i_right += index_increament; } fine_cutoff_i_right = min(fine_cutoff_i_right, detail_level); - if(debug_){ - unsigned int num_scanned_freq_tmp = 0; - for(unsigned int i_eval = 0; i_eval < is_scanned_.size(); ++i_eval){ - num_scanned_freq_tmp += (unsigned int) is_scanned_[i_eval]; - } - cout<<" # of freq scanned for peak finding = "<< num_scanned_freq_tmp - num_scanned_freq< 1){ + cout << " - Do fine scan in the frequency interval ["<< eval_at_frequency[fine_cutoff_i_left] << ", " <>"<< endl; + cout<< " - Fast scan done. Number of frequencies scanned = " << num_scanned << endl; } - is_scanned_.clear(); + if (DEBUG > 2){ + cout << " + Fast scan results:" << endl; + cout << " - eval_at_frequency = linspace(0, 1, "<< eval_at_frequency.size() << ")"<< endl; + cout << " - scanned_index = ["; + for(unsigned int i_eval = 0; i_eval < is_scanned_.size(); ++i_eval) + if(is_scanned_[i_eval]) {cout << i_eval << ", ";} + cout << "]" << endl; + cout << " - scanned_log_posterior_by_frequency = ["; + for(unsigned int i_eval = 0; i_eval < is_scanned_.size(); ++i_eval) + if(is_scanned_[i_eval]) {cout << log_posterior_by_frequency[i_eval] << ", ";} + cout << "]" << endl; + } + is_scanned_.resize(0); + max_ll = max_log_posterior_scanned_; + max_index = (int) argmax_log_posterior_scanned_; +} + +void ScanSpace::UpdateMaxPosteior_(unsigned int i_eval){ + if (log_posterior_by_frequency[i_eval] > max_log_posterior_scanned_){ + argmax_log_posterior_scanned_ = i_eval; + max_log_posterior_scanned_ = log_posterior_by_frequency[argmax_log_posterior_scanned_]; + } +} + + +void ScanSpace::SetTargetMinAlleleFreq(const ExtendParameters& my_param, const vector& variant_specific_params){ + vector all_min_allele_freq; + if (my_param.program_flow.is_multi_min_allele_freq){ + all_min_allele_freq = my_param.program_flow.multi_min_allele_freq; + } + + all_min_allele_freq.reserve(all_min_allele_freq.size() + 4); + if (my_param.my_controls.use_fd_param){ + all_min_allele_freq = {my_param.my_controls.filter_fd_0.min_allele_freq, my_param.my_controls.filter_fd_5.min_allele_freq, my_param.my_controls.filter_fd_10.min_allele_freq}; + }else{ + all_min_allele_freq = {my_param.my_controls.filter_snp.min_allele_freq, my_param.my_controls.filter_mnp.min_allele_freq, my_param.my_controls.filter_hp_indel.min_allele_freq}; + } + if (not my_param.my_controls.hotspots_as_de_novo){ + all_min_allele_freq.push_back(my_param.my_controls.filter_hotspot.min_allele_freq); + } + + for (unsigned int i_allele = 0; i_allele < variant_specific_params.size(); ++i_allele){ + if (variant_specific_params[i_allele].min_allele_freq_override){ + all_min_allele_freq.push_back(variant_specific_params[i_allele].min_allele_freq); + } + } + + int num_steps = 4; + for (vector::iterator f_it = all_min_allele_freq.begin(); f_it != all_min_allele_freq.end(); ++f_it){ + scan_more_frequencies_.push_back(*f_it); + float maf_plus = *f_it + kCoarseFreqResolution_; + maf_plus = (maf_plus > 1.0f) ? 1.0f : maf_plus; + for (int i_step = 0; i_step < num_steps; ++i_step){ + maf_plus = (*f_it + maf_plus) * 0.5f; + scan_more_frequencies_.push_back(min(maf_plus, 1.0f)); // always make sure not scan a frequency > 1.0f + } + float maf_minus = *f_it - kCoarseFreqResolution_; + maf_minus = (maf_minus < 0.0f) ? 0.0f : maf_minus; + for (int i_step = 0; i_step < num_steps; ++i_step){ + maf_minus = (*f_it + maf_minus) * 0.5f; + scan_more_frequencies_.push_back(max(maf_minus, 0.0f)); // always make sure not scan a negative frequency + } + } } + diff --git a/Analysis/VariantCaller/EnsembleEval/PosteriorInference.h b/Analysis/VariantCaller/EnsembleEval/PosteriorInference.h index 9a48ee4e..7e685495 100644 --- a/Analysis/VariantCaller/EnsembleEval/PosteriorInference.h +++ b/Analysis/VariantCaller/EnsembleEval/PosteriorInference.h @@ -35,7 +35,9 @@ class FreqMaster{ float germline_log_prior_normalization; // for comparing LL across different priors vector prior_frequency_weight; - float data_reliability; + // Historically FreqMaster stored and used typical_prob = (1.0f - outlier_prob). + // It can cause singular outlier_prob (i.e. outlier_prob = 0.0f) which is calculated by outlier_prob from outlier_prob = (1.0f - typical_prob) in the downstream process if typical_prob is too close to 1. + float outlier_prob; FreqMaster(); void SetHypFreq(const vector & local_freq); void SetPriorStrength(const vector & local_freq); @@ -58,45 +60,53 @@ class ScanSpace{ // if I am doing one allele vs another for genotyping vector freq_pair; float freq_pair_weight; - bool scan_done; + bool scan_pair_done; + bool scan_ref_done; unsigned int min_detail_level_for_fast_scan; + unsigned int max_detail_level; + int DEBUG; ScanSpace(); float LogDefiniteIntegral(float alpha, float beta); float FindMaxFrequency(); void UpdatePairedFrequency(vector &tmp_freq, FreqMaster &base_clustering, float local_freq); unsigned int ResizeToMatch(ShortStack &total_theory, unsigned max_detail_level = 0); - void DoPosteriorFrequencyScan(ShortStack &total_theory, FreqMaster &base_clustering, bool update_frequency, int strand_key, bool scan_ref, int max_detail_level = 0); - void SetDebug(bool debug){ debug_ = debug;}; + void DoPosteriorFrequencyScan(ShortStack &total_theory, FreqMaster &base_clustering, bool update_frequency, int strand_key, bool scan_ref); + void SetTargetMinAlleleFreq(const ExtendParameters& my_param, const vector& variant_specific_params); private: // Calculate the posterior for just one hyp frequency void DoPosteriorFrequencyScanOneHypFreq_(unsigned int i_eval); + // Scan all frequencies + void DoFullScan_(); + // Update max_log_posterior_scanned_ and argmax_log_posterior_scanned_ + void UpdateMaxPosteior_(unsigned int i_eval); // functions for fast scan void DoFastScan_(); void DoFineScan_(unsigned int i_left, unsigned int i_right, unsigned int i_middle); void DoInterpolation_(); - void LinearInterpolation_(unsigned int i_1, unsigned int i_2, unsigned int i_intp); unsigned int FibonacciSearchMax_(unsigned int i_left, unsigned int i_right); unsigned int FibonacciSearchMax_(unsigned int i_left, unsigned int i_right, unsigned int i_middle); // variables for fast scan - vector is_scanned_; + vector is_scanned_; // is_scanned_[i_eval] = Have I scanned for log_posterior_by_frequency[i_eval]? + vector scan_more_frequencies_; unsigned int argmax_log_posterior_scanned_; float max_log_posterior_scanned_; - float coarse_freq_resolution_; - unsigned int num_of_fibonacci_blocks; - float fine_log_posterior_cutoff_gap_; - float fine_freq_search_increment_; - float min_fine_log_posterior_gap_; - float fine_scan_penalty_order_; + + // hard coded parameters for fast scan + const static unsigned int kNumOfFibonacciBlocks = 3; // The maximums for Hom, Het, Hom + const static constexpr float kCoarseFreqResolution_ = 0.01f; + const static constexpr float kFineLogPosteriorCutoffGap_ = -log(0.00001f); + const static constexpr float kFineFreqSearchIncrement_ = 0.02f; + const static constexpr float kMinFineLogPosteriorGap_ = 0.01f; + const static constexpr float kFineScanPenaltyOrder_ = 1.5f; // pointers for DoPosteriorFrequencyScanOneHypFreq_ ShortStack *ptr_total_theory_ = NULL; FreqMaster *ptr_base_clustering_ = NULL; int *ptr_strand_key_ = NULL; bool *ptr_scan_ref_ = NULL; - bool debug_ = false; }; class PosteriorInference{ @@ -110,6 +120,8 @@ class PosteriorInference{ float params_ll; // likelihood offset for fitted parameters + int DEBUG; + PosteriorInference(); void FindMaxFrequency(bool update_frequency); // from posterior likelihood @@ -125,10 +137,6 @@ class PosteriorInference{ float ReturnMaxLL(){ return(ref_vs_all.max_ll+params_ll);}; float ReturnJustLL(){return(ref_vs_all.max_ll);}; - void SetDebug(bool debug){ - ref_vs_all.SetDebug(debug); - gq_pair.SetDebug(debug); - }; }; diff --git a/Analysis/VariantCaller/EnsembleEval/ShortStack.cpp b/Analysis/VariantCaller/EnsembleEval/ShortStack.cpp index cf69d655..8ec1d204 100644 --- a/Analysis/VariantCaller/EnsembleEval/ShortStack.cpp +++ b/Analysis/VariantCaller/EnsembleEval/ShortStack.cpp @@ -5,11 +5,11 @@ void ShortStack::PropagateTuningParameters(EnsembleEvalTuningParameters &my_params){ - // Set is_molecular_tag_ - for (unsigned int i_read = 0; i_read < my_hypotheses.size(); i_read++) { + for (unsigned int i_read = 0; i_read < my_hypotheses.size(); i_read++) { // basic likelihoods my_hypotheses[i_read].heavy_tailed = my_params.heavy_tailed; + my_hypotheses[i_read].adjust_sigma = my_params.adjust_sigma; // test flow construction my_hypotheses[i_read].max_flows_to_test = my_params.max_flows_to_test; @@ -18,9 +18,6 @@ void ShortStack::PropagateTuningParameters(EnsembleEvalTuningParameters &my_para // used to initialize sigma-estimates my_hypotheses[i_read].magic_sigma_base = my_params.magic_sigma_base; my_hypotheses[i_read].magic_sigma_slope = my_params.magic_sigma_slope; - - // preserve the data for all flows? - my_hypotheses[i_read].preserve_full_data = my_params.preserve_full_data; } } @@ -34,10 +31,13 @@ void ShortStack::FillInPredictionsAndTestFlows(PersistingThreadObjects &thread_o my_hypotheses[i_read].FillInPrediction(thread_objects, *read_stack[i_read], global_context); my_hypotheses[i_read].start_flow = read_stack[i_read]->start_flow; my_hypotheses[i_read].InitializeTestFlows(); + if (not preserve_full_data){ + my_hypotheses[i_read].ClearAllFlowsData(); + } } } -void ShortStack::ResetQualities() { +void ShortStack::ResetQualities(float outlier_probability) { // ! does not reset test flows or delta (correctly) for (unsigned int i_read = 0; i_read < my_hypotheses.size(); i_read++) { my_hypotheses[i_read].InitializeDerivedQualities(); @@ -45,46 +45,50 @@ void ShortStack::ResetQualities() { // reset the derived qualities of my_families if(is_molecular_tag_){ + float uniform_f = (num_hyp_not_null == 0)? 1.0f: 1.0f / (float) num_hyp_not_null; + vector init_hyp_freq(num_hyp_not_null, uniform_f); + // I calculate read responsibility is just for obtaining + UpdateReadResponsibility_(init_hyp_freq, outlier_probability); ResetQualitiesForFamilies(); } } - void ShortStack::ResetQualitiesForFamilies(){ for(unsigned int i_fam = 0; i_fam < my_eval_families.size(); ++i_fam){ // don't count the not functional families - if(my_eval_families[i_fam].GetFunctionality()){ + if(my_eval_families[i_fam].GetFuncFromValid()){ my_eval_families[i_fam].InitializeFamilyResponsibility(); + // Note that I must calculate read responsibility first because now the read log-likeligood propagated to the family is weighted by Resp(read is not an outlier) my_eval_families[i_fam].ComputeFamilyLogLikelihoods(my_hypotheses); } } } -// Call PosteriorFrequencyLogLikelihoodMolTag if is_molecular_tag_ == true -// Call PosteriorFrequencyLogLikelihoodNonMolTag if is_molecular_tag_ == false -float ShortStack::PosteriorFrequencyLogLikelihood(const vector &hyp_freq, const vector &prior_frequency_weight, float prior_log_normalization, float my_reliability, int strand_key) { - return (this->*ptrPosteriorFrequencyLogLikelihood_)(hyp_freq, prior_frequency_weight, prior_log_normalization, my_reliability, strand_key); +// Call PosteriorFrequencyLogLikelihoodFromFamilies_ if is_molecular_tag_ +// Call PosteriorFrequencyLogLikelihoodFromReads_ if not is_molecular_tag_ +float ShortStack::PosteriorFrequencyLogLikelihood(const vector &hyp_freq, const vector &prior_frequency_weight, float prior_log_normalization, float outlier_prob, int strand_key) { + return (this->*ptrPosteriorFrequencyLogLikelihood_)(hyp_freq, prior_frequency_weight, prior_log_normalization, outlier_prob, strand_key); } -float ShortStack::PosteriorFrequencyLogLikelihoodNoMolTags(const vector &hyp_freq, const vector &prior_frequency_weight, float prior_log_normalization, float my_reliability, int strand_key) { +float ShortStack::PosteriorFrequencyLogLikelihoodFromReads_(const vector &hyp_freq, const vector &prior_frequency_weight, float prior_log_normalization, float outlier_prob, int strand_key) { //cout << "eval at freq " << my_freq << endl; - float my_LL = 0.0f; - + double my_LL = 0.0; // use double to calculate cumsum to enjance numerical stability + float my_reliability = 1.0f - outlier_prob; //for (unsigned int i_read=0; i_read &hyp_freq, float data_reliability) { +void ShortStack::UpdateReadResponsibility_(const vector &hyp_freq, float outlier_prob) { //for (unsigned int i_read=0; i_read &hyp_freq, float data_reliability) { - (this->*ptrUpdateResponsibility_)(hyp_freq, data_reliability); +// Call UpdateFamilyAndReadResponsibility_ if is_molecular_tag_ +// Call UpdateReadResponsibility_ if not is_molecular_tag_ +void ShortStack::UpdateResponsibility(const vector &hyp_freq, float outlier_prob) { + (this->*ptrUpdateResponsibility_)(hyp_freq, outlier_prob); } -void ShortStack::MultiFrequencyFromResponsibilityNoMolTags(vector &hyp_freq, vector &prior_frequency_weight, int strand_key){ +void ShortStack::MultiFrequencyFromReadResponsibility_(vector &hyp_freq, vector &prior_frequency_weight, int strand_key){ hyp_freq.assign(hyp_freq.size(), 0.0f); for (unsigned int i_ndx = 0; i_ndx < valid_indexes.size(); i_ndx++) { unsigned int i_read = valid_indexes[i_ndx]; for (unsigned int i_hyp = 0; i_hyp < hyp_freq.size(); i_hyp++) { if ((strand_key < 0) || (my_hypotheses[i_read].strand_key == strand_key)) - hyp_freq[i_hyp] += my_hypotheses[i_read].responsibility[i_hyp+1]; + hyp_freq[i_hyp] += my_hypotheses[i_read].weighted_responsibility[i_hyp+1]; } } // add prior weight to count of cluster @@ -162,158 +166,161 @@ void ShortStack::MultiFrequencyFromResponsibilityNoMolTags(vector &hyp_fr } } -// Call MultiFrequencyFromResponsibilityMolTag if is_molecular_tag_ == true -// Call MultiFrequencyFromResponsibilityNonMolTag if is_molecular_tag_ == false +// Call MultiFrequencyFromFamilyResponsibility_ if is_molecular_tag_ +// Call MultiFrequencyFromReadResponsibility_ if not is_molecular_tag_ void ShortStack::MultiFrequencyFromResponsibility(vector &hyp_freq, vector &prior_frequency_weight, int strand_key){ (this->*ptrMultiFrequencyFromResponsibility_)(hyp_freq, prior_frequency_weight, strand_key); } -void ShortStack::OutlierFiltering(float data_reliability, bool is_update_valid_index){ - vector zeros_vector; - for(unsigned int i_read = 0; i_read < my_hypotheses.size(); ++i_read){ - if(my_hypotheses[i_read].success){ - zeros_vector.assign(my_hypotheses[i_read].responsibility.size(), 0.0f); - // Not yet initialize my_hypotheses. - if(my_hypotheses[i_read].responsibility == zeros_vector){ - my_hypotheses[i_read].InitializeDerivedQualities(); - } - my_hypotheses[i_read].success = (!my_hypotheses[i_read].LocalOutlierClassifier(data_reliability)); - } - } - if(is_update_valid_index){ - FindValidIndexes(); - } -} - -// family stuff // Update Family Responsibility if the Log Likelihoods of reads are updated. -void ShortStack::UpdateFamilyResponsibility_(const vector &hyp_freq, float data_reliability) { - for(unsigned int i_fam = 0; i_fam < my_eval_families.size(); ++i_fam){ - if(my_eval_families[i_fam].GetFunctionality()){ - my_eval_families[i_fam].ComputeFamilyLogLikelihoods(my_hypotheses); - my_eval_families[i_fam].UpdateFamilyResponsibility(hyp_freq, data_reliability); +void ShortStack::UpdateFamilyResponsibility_(const vector &hyp_freq, float outlier_prob) { + for (vector::iterator fam_it = my_eval_families.begin(); fam_it != my_eval_families.end(); ++fam_it){ + if(fam_it->GetFuncFromValid()){ + fam_it->ComputeFamilyLogLikelihoods(my_hypotheses); + fam_it->UpdateFamilyResponsibility(hyp_freq, outlier_prob); + // Note that the outlier responsibility was set to (close to) zero in ComputeFamilyLogLikelihoods and UpdateFamilyResponsibility. + // I am calculating the family outlier responsibility here. + fam_it->ComputeFamilyOutlierResponsibility(my_hypotheses, effective_min_family_size); } } } -// family stuff // Let the family_responsibility be loc_freq // UpdateFamilyResponsibility_() must be done first -void ShortStack::UpdateReadResponsibilityFromFamily_(unsigned int num_hyp_no_null, float data_reliability){ - vector loc_freq; - float safety_zero = 0.00001f; - - loc_freq.assign(num_hyp_no_null, 0.0f); +void ShortStack::UpdateReadResponsibilityFromFamily_(unsigned int num_hyp_no_null, float outlier_prob){ + float safety_zero = 0.00001f; // The close-to-zero outlier prob for the read which is clearly not an outlier. + vector loc_freq(num_hyp_no_null, 0.0f); + // Basically, I let the family responsibility be the prior for the read. + // However, I add a freq offset to the family responsibility to prevent the family bullies a read that has divergent opinion. + // Otherwise the read will be treated as an outlier and won't came back, which may also let the family become an outlier. for(unsigned int i_fam = 0; i_fam < my_eval_families.size(); ++i_fam){ // don't count the non-functional families - if(not my_eval_families[i_fam].GetFunctionality()){ + if(not my_eval_families[i_fam].GetFuncFromValid()){ continue; } - // Let loc_freq be {family_responsibility[1], family_responsibility[2], ...} with a safety zero + float freq_offset = 0.0f; + float freq_offset_gap = 0.01f; // I shall set the parameter outlier_probability << freq_offset_gap. + // Let loc_freq be {family_responsibility[1], family_responsibility[2], ...} with freq_offset float d_normalize = 0.0f; - for (unsigned int i_hyp=0; i_hyp outlier_probability. + freq_offset = (max_loc_freq > outlier_prob) ? outlier_prob + freq_offset_gap * (max_loc_freq - outlier_prob) : outlier_prob; + d_normalize = 1.0f + (freq_offset * (float) num_hyp_no_null); + for (unsigned int i_hyp = 0; i_hyp < num_hyp_no_null; ++i_hyp){ + loc_freq[i_hyp] += freq_offset; + loc_freq[i_hyp] /= d_normalize; } - for(unsigned int i_ndx = 0; i_ndx < my_eval_families[i_fam].family_members.size(); ++i_ndx){ - int i_read = my_eval_families[i_fam].family_members[i_ndx]; - my_hypotheses[i_read].UpdateResponsibility(loc_freq, data_reliability); + for (unsigned int i_ndx = 0; i_ndx < my_eval_families[i_fam].valid_family_members.size(); ++i_ndx){ + int i_read = my_eval_families[i_fam].valid_family_members[i_ndx]; + float my_outlier_prob = my_hypotheses[i_read].at_least_one_same_as_null ? safety_zero: outlier_prob; + my_hypotheses[i_read].UpdateResponsibility(loc_freq, my_outlier_prob); } } } -// family stuff // pretty much the same as void ShortStack::MultiFrequencyFromResponsibility(...) except we use the family responsibility void ShortStack::MultiFrequencyFromFamilyResponsibility_(vector &hyp_freq, vector &prior_frequency_weight, int strand_key){ hyp_freq.assign(hyp_freq.size(), 0.0f); - for(unsigned int i_fam = 0; i_fam < my_eval_families.size(); ++ i_fam){ if(strand_key >= 0 and my_eval_families[i_fam].strand_key != strand_key){ continue; } // don't count the not functional families - if(!(my_eval_families[i_fam].GetFunctionality())){ + if(not my_eval_families[i_fam].GetFuncFromValid()){ continue; } - for (unsigned int i_hyp=0; i_hyp &hyp_freq, const vector &prior_frequency_weight, float prior_log_normalization, float my_reliability, int strand_key) { - float my_LL = 0.0f; - +float ShortStack::PosteriorFrequencyLogLikelihoodFromFamilies_(const vector &hyp_freq, const vector &prior_frequency_weight, float prior_log_normalization, float outlier_prob, int strand_key) { + double my_LL = 0.0; // use double to calculate cumsum to enjance numerical stability + float my_reliability = 1.0f - outlier_prob; for(unsigned int i_fam = 0; i_fam < my_eval_families.size(); ++i_fam){ if(strand_key >= 0 and my_eval_families[i_fam].strand_key != strand_key){ continue; } // don't count the non-functional families - if(!(my_eval_families[i_fam].GetFunctionality())){ + if(!(my_eval_families[i_fam].GetFuncFromValid())){ continue; } - - my_LL += my_eval_families[i_fam].ComputeFamilyPosteriorLikelihood(hyp_freq, my_reliability); + my_LL += (double) my_eval_families[i_fam].ComputeFamilyPosteriorLikelihood(hyp_freq); } // add contribution from prior for (unsigned int i_hyp=0; i_hyp &hyp_freq, const vector &prior_frequency_weight, float prior_log_normalization, float my_reliability, int strand_key){ - // the posterior likelihood of hyp_freq is the posterior likelihood of hyp_freq from families - return PosteriorFrequencyFamilyLogLikelihood_(hyp_freq, prior_frequency_weight, prior_log_normalization, my_reliability, strand_key); -} - -void ShortStack::UpdateResponsibilityMolTags(const vector &hyp_freq, float data_reliability){ - // first update family responsibility - UpdateFamilyResponsibility_(hyp_freq, data_reliability); - // then update read responsibility from family - UpdateReadResponsibilityFromFamily_(hyp_freq.size(), data_reliability); -} - -void ShortStack::MultiFrequencyFromResponsibilityMolTags(vector &hyp_freq, vector &prior_frequency_weight, int strand_key){ - // Update hyp_freq using family responsibility - MultiFrequencyFromFamilyResponsibility_(hyp_freq, prior_frequency_weight, strand_key); +// A belief propagation approach +//@TODO: The outlier handling can be further improved. +void ShortStack::UpdateFamilyAndReadResponsibility_(const vector &hyp_freq, float outlier_prob){ + // 1. update family responsibility + UpdateFamilyResponsibility_(hyp_freq, outlier_prob); + // 2. update read responsibility from family + UpdateReadResponsibilityFromFamily_(hyp_freq.size(), outlier_prob); } void ShortStack::SwitchMolTagsPtr_(void){ if(is_molecular_tag_){ - ptrPosteriorFrequencyLogLikelihood_ = &ShortStack::PosteriorFrequencyLogLikelihoodMolTags; - ptrUpdateResponsibility_ = &ShortStack::UpdateResponsibilityMolTags; - ptrMultiFrequencyFromResponsibility_ = &ShortStack::MultiFrequencyFromResponsibilityMolTags; + // I do inference for the allele frequency from families + ptrPosteriorFrequencyLogLikelihood_ = &ShortStack::PosteriorFrequencyLogLikelihoodFromFamilies_; + ptrUpdateResponsibility_ = &ShortStack::UpdateFamilyAndReadResponsibility_; + ptrMultiFrequencyFromResponsibility_ = &ShortStack::MultiFrequencyFromFamilyResponsibility_; } else{ - ptrPosteriorFrequencyLogLikelihood_ = &ShortStack::PosteriorFrequencyLogLikelihoodNoMolTags; - ptrUpdateResponsibility_ = &ShortStack::UpdateResponsibilityNoMolTags; - ptrMultiFrequencyFromResponsibility_ = &ShortStack::MultiFrequencyFromResponsibilityNoMolTags; + // I do inference for the allele frequency from reads + ptrPosteriorFrequencyLogLikelihood_ = &ShortStack::PosteriorFrequencyLogLikelihoodFromReads_; + ptrUpdateResponsibility_ = &ShortStack::UpdateReadResponsibility_; + ptrMultiFrequencyFromResponsibility_ = &ShortStack::MultiFrequencyFromReadResponsibility_; } } @@ -323,37 +330,69 @@ void ShortStack::SetIsMolecularTag(bool is_mol_tag){ SwitchMolTagsPtr_(); } - unsigned int ShortStack::DetailLevel(void){ - if(is_molecular_tag_) - return num_func_families_; + if (is_molecular_tag_) + // With mol tagging, we often call variants using just a few (<5) variant families. + // If I serve detail_level = num_func_families_, then the singularity of log-posterior at f = 0 may cause big interpolation error. + // Thus I let detail_level finer than num_func_families_to obtain better numerical accuracy. + // Since the number of scanned points doesn't grow a lot in fast scan, the complexity is manageable. + // (Note): Setting detail_level to be too large (e.g. num_valid_read_counts_) may cause floating point error on frequency and cause problem in Fibonacci search dueing fast scan. + return num_func_families_ * 2; // This should be enough. return my_hypotheses.size(); } -// Set the functionality for all families in my_families. -void ShortStack::SetFuncionalityForFamilies(unsigned int min_fam_size){ +// Step 1): Initialize all families +// Step 2): Set the functionality of the families in my_families. +// Step 3): Update valid_index +void ShortStack::InitializeMyEvalFamilies(unsigned int num_hyp){ num_func_families_ = 0; - for(vector::iterator it_fam = my_eval_families.begin(); - it_fam != my_eval_families.end(); ++it_fam){ - vector fam_members_temp(0); - fam_members_temp.reserve(it_fam->family_members.size()); - for(vector::iterator it_read = it_fam->family_members.begin(); - it_read !=it_fam->family_members.end(); ++it_read){ - if(my_hypotheses[*it_read].success){ - fam_members_temp.push_back(*it_read); + for (vector::iterator fam_it = my_eval_families.begin(); fam_it != my_eval_families.end(); ++fam_it){ + fam_it->InitializeEvalFamily(num_hyp); + fam_it->ResetValidFamilyMembers(); + for (vector::iterator read_it = fam_it->all_family_members.begin(); read_it != fam_it->all_family_members.end(); ++read_it){ + if (my_hypotheses[*read_it].success){ + // valid_family_members contains only the reads that are successfully initialized + fam_it->valid_family_members.push_back(*read_it); } } - it_fam->family_members.swap(fam_members_temp); - if(not it_fam->SetFunctionality(min_fam_size)){ + fam_it->CountFamSizeFromValid(); + if (fam_it->SetFuncFromValid(effective_min_family_size)){ + ++num_func_families_; + num_valid_read_counts_ += fam_it->GetValidFamSize(); + } + else{ // disable the reads in a non-functional family - for(vector::iterator it_read = it_fam->family_members.begin(); - it_read !=it_fam->family_members.end(); ++it_read){ - my_hypotheses[*it_read].success = false; + for(vector::iterator read_it = fam_it->valid_family_members.begin(); read_it !=fam_it->valid_family_members.end(); ++read_it){ + my_hypotheses[*read_it].success = false; } - }else{ - ++num_func_families_; } } + + // Some of the reads may be set to not success. Need to update valid_index. FindValidIndexes(); } + +int ShortStack::OutlierCountsByFlowDisruptiveness(){ + int ol_counts = 0; + for (unsigned int i_read = 0; i_read < my_hypotheses.size(); ++i_read){ + ol_counts += my_hypotheses[i_read].OutlierByFlowDisruptiveness(); + } + if (DEBUG > 0){ + cout << endl << "+ Counting outlier reads using flow-disruptiveness:" < &prediction, float responsibility, float skew_estimate, vector &test_flow, vector &residuals); + void AddOneUpdateForHypothesis(vector &prediction, float responsibility, float skew_estimate, vector &test_flow, vector &residuals, vector &measurements_var); void DoLatentUpdate(); float RetrieveApproximateWeight(float x_val); void PushToPrior(); @@ -81,13 +81,17 @@ class StrandedSigmaGenerator{ BasicSigmaGenerator fwd,rev; // if want to revert to old-style bool combine_strands; - - StrandedSigmaGenerator(){combine_strands = false;}; + int DEBUG = 0; + StrandedSigmaGenerator(){ + combine_strands = false; + DEBUG = 0; + }; void UpdateSigmaGenerator(ShortStack &total_theory); void UpdateSigmaEstimates(ShortStack &total_theory); void DoStepForSigma(ShortStack &total_theory); void ResetSigmaGenerator(); + void PrintDebug(bool print_update = true); }; diff --git a/Analysis/VariantCaller/EnsembleEval/SkewGenerator.cpp b/Analysis/VariantCaller/EnsembleEval/SkewGenerator.cpp index 47da4587..1b13ffdd 100644 --- a/Analysis/VariantCaller/EnsembleEval/SkewGenerator.cpp +++ b/Analysis/VariantCaller/EnsembleEval/SkewGenerator.cpp @@ -23,7 +23,7 @@ void BasicSkewGenerator::AddOneUpdateForHypothesis(int strand_key, float respons void BasicSkewGenerator::AddCrossUpdate(CrossHypotheses &my_cross){ for (unsigned int i_hyp=1; i_hyp a, pair b){ @@ -10,12 +12,12 @@ bool compare_best_response(pair a, pair b){ void LatentSlate::PropagateTuningParameters(EnsembleEvalTuningParameters &my_params, int num_hyp_no_null) { // prior reliability for outlier read frequency - cur_posterior.clustering.data_reliability = my_params.DataReliability(); + cur_posterior.clustering.outlier_prob = my_params.outlier_prob; cur_posterior.clustering.germline_prior_strength = my_params.germline_prior_strength; + cur_posterior.gq_pair.max_detail_level = (unsigned int) my_params.max_detail_level; cur_posterior.gq_pair.min_detail_level_for_fast_scan = (unsigned int) my_params.min_detail_level_for_fast_scan; cur_posterior.ref_vs_all.min_detail_level_for_fast_scan = (unsigned int) my_params.min_detail_level_for_fast_scan; - //rev_posterior.data_reliability = my_params.DataReliability(); - //fwd_posterior.data_reliability = my_params.DataReliability(); + cur_posterior.ref_vs_all.max_detail_level = (unsigned int) my_params.max_detail_level; // prior precision and likelihood penalty for moving off-center bias_generator.InitForStrand(num_hyp_no_null-1); // num_alt = num_hyp_no_null-1 @@ -38,6 +40,16 @@ void LatentSlate::PropagateTuningParameters(EnsembleEvalTuningParameters &my_par skew_generator.dampened_skew = my_params.prediction_precision; } +void LatentSlate::SetAndPropagateDebug(int debug) { + DEBUG = debug; + cur_posterior.DEBUG = debug; + cur_posterior.ref_vs_all.DEBUG = debug; + cur_posterior.gq_pair.DEBUG = debug; + bias_generator.DEBUG = debug; + sigma_generator.DEBUG = debug; +} + + // see how quick we can make this void LatentSlate::LocalExecuteInference(ShortStack &total_theory, bool update_frequency, bool update_sigma, vector &start_frequency) { if (detailed_integral) { @@ -51,6 +63,9 @@ void LatentSlate::LocalExecuteInference(ShortStack &total_theory, bool update_fr void LatentSlate::FastStep(ShortStack &total_theory, bool update_frequency, bool update_sigma) { + if(DEBUG > 0){ + cout << " - Starting one iteration with allele_freq = " << PrintIteratorToString(cur_posterior.clustering.max_hyp_freq.begin(), cur_posterior.clustering.max_hyp_freq.end()) << (update_frequency? "." : ", update_frequency = false.") << endl; + } bias_generator.DoStepForBias(total_theory); // update bias estimate-> residuals->likelihoods if (update_frequency) @@ -60,7 +75,9 @@ void LatentSlate::FastStep(ShortStack &total_theory, bool update_frequency, bool sigma_generator.DoStepForSigma(total_theory); // update sigma estimate if (update_frequency) cur_posterior.QuickUpdateStep(total_theory); - + } + if(DEBUG > 0){ + cout << " - One iteration done: ref_vs_all.max_ll = "<< cur_posterior.ReturnJustLL() < fake_hs_alleles; + fake_hs_alleles.reserve(num_hyp_no_null - 1); // try pure frequencies for the alleles if(try_alternatives){ // try pure frequencies for alt alleles for(int i_hyp = 1; i_hyp < num_hyp_no_null; ++i_hyp){ int i_alt = i_hyp - 1; + if (allele_identity_vector[i_alt].status.isFakeHsAllele){ + // I don't want to try the pure freq for a fake hs allele/ + fake_hs_alleles.push_back(i_hyp); + continue; + } if(my_params.try_few_restart_freq and (allele_identity_vector[i_alt].ActAsSNP() or allele_identity_vector[i_alt].ActAsMNP())){ // skip the pure frequency of a snp or mnp alt allele. continue; } + try_me.assign(num_hyp_no_null, safety_zero / float(num_hyp_no_null - 1)); try_me[i_hyp] = 1.0f - safety_zero; try_hyp_freq.push_back(try_me); } + + bool has_ref_pure = false; // try the pure frequency of the ref allele if we try at least one pure frequency of alt allele - if(try_hyp_freq.size() > 1){ + if (try_hyp_freq.size() > 1){ try_me.assign(num_hyp_no_null, safety_zero / float(num_hyp_no_null - 1)); try_me[0] = 1.0f - safety_zero; try_hyp_freq.push_back(try_me); + has_ref_pure = true; + } + + // Finally, try the uniform allele freq precluding all fake HS alleles. + if (not fake_hs_alleles.empty()){ + int num_non_fake_hs_alleles = num_hyp_no_null - (int) fake_hs_alleles.size(); + if (num_non_fake_hs_alleles > 1 or (num_non_fake_hs_alleles == 1 and (not has_ref_pure))){ + try_me.assign(num_hyp_no_null, (1.0f - safety_zero) / (float) num_non_fake_hs_alleles); + float my_safety_zero = safety_zero / (float) fake_hs_alleles.size(); + for (unsigned int idx = 0; idx < fake_hs_alleles.size(); ++idx){ + try_me[fake_hs_alleles[idx]] = my_safety_zero; + } + try_hyp_freq.push_back(try_me); + } } } @@ -187,26 +224,64 @@ float HypothesisStack::ReturnMaxLL() { return cur_state.cur_posterior.ReturnMaxLL(); } -void HypothesisStack::InitForInference(PersistingThreadObjects &thread_objects, vector& read_stack, const InputStructures &global_context, int num_hyp_no_null, vector &allele_identity_vector) { +void HypothesisStack::InitForInference(PersistingThreadObjects &thread_objects, vector& read_stack, const InputStructures &global_context, vector &allele_identity_vector) { + int num_hyp_no_null = (int) allele_identity_vector.size() + 1; // number of alt alleles + 1 for ref + total_theory.num_hyp_not_null = num_hyp_no_null; PropagateTuningParameters(num_hyp_no_null); // sub-objects need to know - cur_state.cur_posterior.SetDebug(global_context.DEBUG > 1); // debug information for fast scan - // predict given hypotheses per read - total_theory.FillInPredictionsAndTestFlows(thread_objects, read_stack, global_context); - total_theory.FindValidIndexes(); // how many alleles? AllocateFrequencyStarts(num_hyp_no_null, allele_identity_vector); + // predict given hypotheses per read + total_theory.FillInPredictionsAndTestFlows(thread_objects, read_stack, global_context); + if(not total_theory.GetIsMolecularTag()){ + // If use mol tag, total_theory.FindValidIndexes() is done in total_theory.InitializeMyEvalFamilies(unsigned int num_hyp) + total_theory.FindValidIndexes(); + } + else{ + total_theory.FlowDisruptiveOutlierFiltering(false); // Filter out obvious outlier reads using flow-disruption. + total_theory.InitializeMyEvalFamilies((unsigned int) num_hyp_no_null + 1); + + if(DEBUG > 0){ + cout << endl << "+ Initialized families on the read stack: "<< endl + <<" - Number of reads on the read stack = " << total_theory.my_hypotheses.size() << endl + <<" - Number of valid reads on the read stack = " << total_theory.valid_indexes.size() << endl + <<" - Number of families on the read stack = "<< total_theory.my_eval_families.size() << endl + <<" - Number of functional families on the read stack = "<< total_theory.GetNumFuncFamilies() << endl; + } + } } void HypothesisStack::ExecuteInference() { + unsigned long t0 = clock(); + + if(DEBUG > 0){ + cout << endl << "+ Execute inference for the variant (" << PrintVariant(*variant) << ")" << endl; + } + // now with unrestrained inference ExecuteExtremeInferences(); // set up our filter cur_state.bias_checker.UpdateBiasChecker(total_theory); // @TODO: Now with fast scan, we can always do it - if(my_params.max_detail_level > 0){ - cur_state.ScanStrandPosterior(total_theory, true, my_params.max_detail_level); + if(cur_state.cur_posterior.ref_vs_all.max_detail_level > 0){ + cur_state.ScanStrandPosterior(total_theory, true); } + + if(DEBUG > 0){ + vector max_allele_freq = cur_state.cur_posterior.clustering.max_hyp_freq; + if(cur_state.cur_posterior.ref_vs_all.scan_ref_done){ + cur_state.cur_posterior.clustering.UpdateFrequencyAgainstOne(max_allele_freq, cur_state.cur_posterior.ref_vs_all.eval_at_frequency[cur_state.cur_posterior.ref_vs_all.max_index], 0); + } + cout << "+ Execute inference for the variant (" << PrintVariant(*variant) << ") done. " + << "Processing time = " << (double) (clock() - t0) / 1E6 << " sec."<< endl + << " - Winner of the initial allele_freq = " << PrintIteratorToString(cur_state.start_freq_of_winner.begin(), cur_state.start_freq_of_winner.end()) << endl; + cur_state.bias_generator.PrintDebug(false); + cur_state.sigma_generator.PrintDebug(false); + cout << " - params_ll = "<< cur_state.cur_posterior.params_ll << endl + << " - ref_vs_all.max_ll + params_ll = "<< cur_state.cur_posterior.ReturnMaxLL() <<" @ allele_freq = " << PrintIteratorToString(max_allele_freq.begin(), max_allele_freq.end()) + << (cur_state.cur_posterior.ref_vs_all.scan_ref_done? " from scan." : "from responsibility.") << endl << endl; + } + } void HypothesisStack::SetAlternateFromMain() { @@ -218,24 +293,42 @@ void HypothesisStack::SetAlternateFromMain() { -float HypothesisStack::ExecuteOneRestart(vector &restart_hyp, int max_detail_level){ +float HypothesisStack::ExecuteOneRestart(vector &restart_hyp){ LatentSlate tmp_state; tmp_state = cur_state; tmp_state.detailed_integral = false; - tmp_state.start_freq_of_winner =restart_hyp; - total_theory.ResetQualities(); // clean slate to begin again + tmp_state.start_freq_of_winner = restart_hyp; + total_theory.ResetQualities(my_params.outlier_prob); // clean slate to begin again tmp_state.ResetToOrigin(); // everyone back to starting places + if (DEBUG > 0){ + cout<< "+ Restart the EM algorithm with initial allele_freq = " << PrintIteratorToString(restart_hyp.begin(), restart_hyp.end()) << endl; + } tmp_state.LocalExecuteInference(total_theory, true, true, restart_hyp); // start at reference - if(max_detail_level < 1){ - tmp_state.ScanStrandPosterior(total_theory, true, 0); + + if( tmp_state.cur_posterior.ref_vs_all.max_detail_level == 0){ + if (DEBUG > 0){ + cout<< " - Scan posterior likelihood for the restart of the EM algorithm."<< endl; + } + tmp_state.ScanStrandPosterior(total_theory, true); } float restart_LL=tmp_state.cur_posterior.ReturnMaxLL(); + if (DEBUG > 0){ + vector max_allele_freq = tmp_state.cur_posterior.clustering.max_hyp_freq; + if(tmp_state.cur_posterior.ref_vs_all.scan_ref_done){ + tmp_state.cur_posterior.clustering.UpdateFrequencyAgainstOne(max_allele_freq, tmp_state.cur_posterior.ref_vs_all.eval_at_frequency[tmp_state.cur_posterior.ref_vs_all.max_index], 0); + } + cout << "+ Restart the EM algorithm with initial allele_freq = " << PrintIteratorToString(restart_hyp.begin(), restart_hyp.end()) << " done. "<< endl + << " - params_ll = "<< tmp_state.cur_posterior.params_ll << endl + << " - ref_vs_all.max_ll + params_ll = "<< restart_LL << " @ allele_freq = " << PrintIteratorToString(max_allele_freq.begin(), max_allele_freq.end()) << (tmp_state.cur_posterior.ref_vs_all.scan_ref_done? " from scan." : "from responsibility.") << endl; + } + if (cur_state.cur_posterior.ReturnMaxLL() 0){ + cout<< " - The EM algorithm will be restarted by trying "<< try_hyp_freq.size() << " different initial allele_freq."<< endl; + } for (unsigned int i_start=0; i_start 0){ + int pair_0 = cur_posterior.gq_pair.freq_pair[0]; + int pair_1 = cur_posterior.gq_pair.freq_pair[1]; + float allele_ratio_cut_off = real_safety / (1.0f - real_safety); + cout << "+ Calling genotype: " << endl; + cout << " - Baseline allele_freq = " << PrintIteratorToString(cur_posterior.clustering.max_hyp_freq.begin(), cur_posterior.clustering.max_hyp_freq.end()) << endl; + cout << " - H(GT="<< pair_0 <<"/"<< pair_0 + << ": allele_freq[" << pair_1 <<"]/(allele_freq["<< pair_0 << "]+allele_freq[" << pair_1 << "])" + << " < " << real_safety << "), log(definite integral of posterior) = " << genotype_interval[0] < " << real_safety << "), log(definite integral of posterior) = " << genotype_interval[2] << endl; + cout << " - Call GT = "; + switch (genotype_call){ + case 0: + cout << pair_0 <<"/"<< pair_0; + break; + case 1: + cout << pair_0 <<"/"<< pair_1; + break; + case 2: + cout << pair_1 <<"/"<< pair_1; + break; + default: + cout << "?/?"; + break; + } + cout << ", GQ = " << quasi_phred_quality_score << endl; + } + } bool RejectionByIntegral(vector dual_interval, float &reject_status_quality_score){ // reject ref = quality of rejection call - bool is_ref = false; - float log_ref = 0.0f; - int top = 0; - int bottom = 1; - - // First normalize dual_interval to enhance the floating point accuracy - float max_dual_interval = max(dual_interval[0], dual_interval[1]); - for (unsigned int i = 0; i < dual_interval.size(); ++i){ - dual_interval[i] -= max_dual_interval;} - - if (dual_interval[1]>dual_interval[0]) { - // if reference, how strongly can we reject the outer interval - log_ref = dual_interval[0]; - is_ref = false; - top=1; - bottom=0; - } - else { - // if var, how strongly can we reject ref - log_ref = dual_interval[1]; - is_ref = true; - top=0; - bottom=1; - } + bool is_ref = dual_interval[0] >= dual_interval[1]; + double dual_interval_diff = abs((double) dual_interval[0] - (double) dual_interval[1]); // Use double to enhance the numerical accuracy + // QUAL = exp(min(dual_interval)) / ( exp(min(dual_interval)) + exp(max(dual_interval) ) ) in phred scale which is equivalent to the following expression. + reject_status_quality_score = (float) (10.0 / log(10.0) * (dual_interval_diff + log(1.0 + exp(-dual_interval_diff)))); + reject_status_quality_score = max(reject_status_quality_score, 3.0103f); // Guard by the minimum possible QUAL score to prevent floating point error. - float log_all = dual_interval[top]+ log(1.0f+exp(dual_interval[bottom]-dual_interval[top])); - - reject_status_quality_score = 10.0f * (log_all - log_ref) / log(10.0f); // how much mass speaks against the pure reference state - reject_status_quality_score = max(reject_status_quality_score, 3.010f); // Prevent floating error that causes QUAL < 10*log10(2) - if (isnan(reject_status_quality_score)) { - cout << "Warning: reject ref score NAN " << endl; - reject_status_quality_score = 0.0f; - } return is_ref; } @@ -424,16 +524,22 @@ bool DoRejectionByIntegral(PosteriorInference &cur_posterior, float real_safety, dual_interval[i_cut] = cur_posterior.ref_vs_all.LogDefiniteIntegral(variant_cuts[i_cut+1], variant_cuts[i_cut]); } - return RejectionByIntegral(dual_interval, reject_status_quality_score); + bool is_ref = RejectionByIntegral(dual_interval, reject_status_quality_score); + if (cur_posterior.DEBUG > 0){ + cout << "+ Calling ref vs. (all alt): "<< endl; + cout << " - Baseline allele_freq = " << PrintIteratorToString(cur_posterior.clustering.max_hyp_freq.begin(), cur_posterior.clustering.max_hyp_freq.end()) << endl; + float min_area = min(dual_interval[0], dual_interval[1]); + cout << " - H(ref: sum(allele_freq[1:])/allele_freq[0] < "<< real_safety << "/" << (1.0f - real_safety) + << " = " << real_safety / (1.0f - real_safety) << "), log(definite integral of posterior) = " << min_area <<" + " << (dual_interval[0] - min_area) << endl; + cout << " - H(all alt: sum(allele_freq[1:])/allele_freq[0] >= "<< real_safety << "/" << (1.0f - real_safety) + << " = " << real_safety / (1.0f - real_safety) << "), log(definite integral of posterior) = " << min_area <<" + " << (dual_interval[1] - min_area) << endl; + cout << " - "<< (is_ref? "Call reference" : "Call variant")<< ", QUAL = " << reject_status_quality_score << endl; + } + return is_ref; } -// must have scan done to be workable -// must have set which pair of hypotheses are being checked -bool CallByIntegral(PosteriorInference &cur_posterior, float hom_safety, int &genotype_call, float &quasi_phred_quality_score, float &reject_status_quality_score) { - // divide MAF into three zones = 0/1/2 variant alleles - +bool CalculateRealSafetyAfCutoff(float af_cutoff, int detail_level, float &real_safety){ // make sure we're safe based on the number of reads - int detail_level = cur_posterior.ref_vs_all.eval_at_frequency.size(); // if we don't have enough reads might be crazy near mid float fine_scale = 0.5f / (detail_level + 1.0f); float mid_cutoff = 0.5f - fine_scale; @@ -441,29 +547,61 @@ bool CallByIntegral(PosteriorInference &cur_posterior, float hom_safety, int &ge // so no sense testing that low float low_cutoff = 1.0f / detail_level; // if we have a small number of reads, these ranges may conflict - float real_safety = min(mid_cutoff, max(low_cutoff, hom_safety)); + real_safety = min(mid_cutoff, max(low_cutoff, af_cutoff)); + bool safety_active_flag = false; + if (fabs(real_safety- af_cutoff) > fine_scale) + safety_active_flag = true; + return safety_active_flag; +} +// must have scan done to be workable +// must have set which pair of hypotheses are being checked +bool HypothesisStack::CallByIntegral(float af_cutoff_rej, float af_cutoff_gt, vector &genotype_component, float &quasi_phred_quality_score, float &reject_status_quality_score, int &qual_type) { + // divide MAF into three zones = 0/1/2 variant alleles bool safety_active_flag = false; - if (fabs(real_safety - hom_safety) > fine_scale) - safety_active_flag = true; + int detail_level = cur_state.cur_posterior.ref_vs_all.eval_at_frequency.size(); - // bool isref = - DoRejectionByIntegral(cur_posterior, real_safety, reject_status_quality_score); + float real_safety_rej = af_cutoff_rej; + safety_active_flag += CalculateRealSafetyAfCutoff(af_cutoff_rej, detail_level, real_safety_rej); - // in dual allele case, do not need to check "is-ref" before making some quality assessment - DoGenotypeByIntegral(cur_posterior, real_safety, genotype_call, quasi_phred_quality_score); - //cout << genotype_call << "\t" << reject_status_quality_score << "\t" << quasi_phred_quality_score << endl; + bool is_ref = DoRejectionByIntegral(cur_state.cur_posterior, real_safety_rej, reject_status_quality_score); + qual_type = is_ref? 0 : 1; - return safety_active_flag; -} + /* Less exception is better, though it is possible to see some non-trivial cases. + // If I reject the variant, I force to make a reference call (i.e., GT=0/0) and output GQ=QUAL. + // In this case, I don't even try to scan the gq pair and call DoGenotypeByIntegral. + if (is_ref){ + quasi_phred_quality_score = reject_status_quality_score; + genotype_component.assign(2, 0); + if (cur_state.cur_posterior.DEBUG > 0){ + cout << "+ Set GT=0/0 and GQ=QUAL for a reference call." << endl; + } + return safety_active_flag; + } + */ -bool HypothesisStack::CallGermline(float hom_safety, int &genotype_call, float &quasi_phred_quality_score, float &reject_status_quality_score){ - bool retval = CallByIntegral(cur_state.cur_posterior, hom_safety, genotype_call, quasi_phred_quality_score, reject_status_quality_score); - return retval; -} + int genotype_call = 0; + assert(not cur_state.cur_posterior.gq_pair.freq_pair.empty()); // gq_pair.freq_pair must be set. + if (not cur_state.cur_posterior.gq_pair.scan_pair_done){ + cur_state.cur_posterior.gq_pair.DoPosteriorFrequencyScan(total_theory, cur_state.cur_posterior.clustering, true, ALL_STRAND_KEY, false); + } + float real_safety_gt = af_cutoff_gt; + safety_active_flag += CalculateRealSafetyAfCutoff(af_cutoff_gt, detail_level, real_safety_gt); + // in dual allele case, do not need to check "is-ref" before making some quality assessment + DoGenotypeByIntegral(cur_state.cur_posterior, real_safety_gt, genotype_call, quasi_phred_quality_score); + + genotype_component = cur_state.cur_posterior.gq_pair.freq_pair; // het diploid_choice[0]/diploid_choice[1] + if(genotype_call == 2){ // hom diploid_choice[1]/diploid_choice[1] + genotype_component[0] = genotype_component[1]; + } + else if(genotype_call == 0){ // hom diploid_choice[0]/diploid_choice[0] + genotype_component[1] = genotype_component[0]; + } + return safety_active_flag; +} // evidence for i_allele vs ref void EnsembleEval::ScanSupportingEvidence(float &mean_ll_delta, int i_allele) { @@ -473,15 +611,24 @@ void EnsembleEval::ScanSupportingEvidence(float &mean_ll_delta, int i_allele) { int ref_hyp = 1; int alt_hyp = i_allele + 2; // alt_alleles = 0->n not counting ref >or< null, therefore alt-allele 0 = 2 - for (unsigned int i_read = 0; i_read < allele_eval.total_theory.my_hypotheses.size(); i_read++) { - if (allele_eval.total_theory.my_hypotheses[i_read].success) { - // measure disruption - - mean_ll_delta += allele_eval.total_theory.my_hypotheses[i_read].ComputeLLDifference(ref_hyp, alt_hyp); - count++; - } + if (allele_eval.total_theory.GetIsMolecularTag()){ + for (vector::iterator fam_it = allele_eval.total_theory.my_eval_families.begin(); fam_it != allele_eval.total_theory.my_eval_families.end(); ++fam_it){ + if (fam_it->GetFuncFromValid()){ + mean_ll_delta += fam_it->ComputeLLDifference(ref_hyp, alt_hyp); + ++count; + } + } } - mean_ll_delta /= (count + 0.01f); + else{ + for (vector::iterator read_it = allele_eval.total_theory.my_hypotheses.begin(); read_it != allele_eval.total_theory.my_hypotheses.end(); ++read_it){ + if (read_it->success){ + mean_ll_delta += read_it->ComputeLLDifference(ref_hyp, alt_hyp); + ++count; + } + } + } + + mean_ll_delta /= ((float) count + 0.01f); mean_ll_delta = 10.0f * mean_ll_delta / log(10.0f); // phred-scaled } @@ -489,23 +636,19 @@ void EnsembleEval::ScanSupportingEvidence(float &mean_ll_delta, int i_allele) { // read_id_[i] = -1 means the i-th read is classified as an outlier. // read_id_[i] = 0 means the i-th read is classified as ref. // read_id_[i] = 1 means the i-th read is classified as the variant allele 1, and so on. -void EnsembleEval::ApproximateHardClassifierForReads(){ - read_id_.clear(); - strand_id_.clear(); - dist_to_left_.clear(); - dist_to_right_.clear(); - +void EnsembleEval::ApproximateHardClassifierForReads() +{ read_id_.assign(read_stack.size(), -1); strand_id_.assign(read_stack.size(), false); dist_to_left_.assign(read_stack.size(), -1); dist_to_right_.assign(read_stack.size(), -1); int position0 = variant->position -1; // variant->position 1-base: vcflib/Variant.h - for (unsigned int i_read = 0; i_read < read_stack.size(); ++i_read) { // compute read_id_ if(allele_eval.total_theory.my_hypotheses[i_read].success){ - read_id_[i_read] = allele_eval.total_theory.my_hypotheses[i_read].MostResponsible() - 1; // -1 = null, 0 = ref , ... + int most_responsibile_hyp = allele_eval.total_theory.my_hypotheses[i_read].MostResponsible(); + read_id_[i_read] = most_responsibile_hyp - 1; // -1 = null, 0 = ref , ... } else{ read_id_[i_read] = -1; // failure = outlier @@ -523,47 +666,178 @@ void EnsembleEval::ApproximateHardClassifierForReads(){ //compute strand_id_ strand_id_[i_read] = not (read_stack[i_read]->is_reverse_strand); } +} - is_hard_classification_for_reads_done_ = true; +struct PositionGroup{ + vector group_members; + uint16_t mapq_sum; + int group_size; + int group_position; + PositionGroup(){ mapq_sum = 0; group_size = 0; group_position = 0;}; + PositionGroup(const Alignment* rai, int p){ + group_members = {rai}; + mapq_sum = rai->alignment.MapQuality; + group_size = rai->read_count; + group_position = p; + }; + void AddNew(const Alignment* rai){ + group_members.push_back(rai); + mapq_sum += rai->alignment.MapQuality; + group_size += rai->read_count; + }; + // Compare two groups. + // A group wins if it has more reads. If ties, the group with larger accumulated mapping quality wins. + bool operator>(const PositionGroup& rhs){ + if (group_size == rhs.group_size){ + return (mapq_sum > rhs.mapq_sum); + } + return (group_size > rhs.group_size); + }; +}; + +int GetConsensusPosition(EnsembleEval* my_ensemble, EvalFamily* my_fam, bool is_to_left){ + if (not my_fam->GetFuncFromValid()){ + return -1; + } + int position0 = my_ensemble->variant->position -1; // variant->position 1-base: vcflib/Variant.h + vector position_groups_vec; + position_groups_vec.reserve(my_fam->valid_family_members.size()); + for (vector::iterator member_it = my_fam->valid_family_members.begin(); member_it != my_fam->valid_family_members.end(); ++member_it) + { + if (not my_ensemble->allele_eval.total_theory.my_hypotheses[*member_it].success){ + continue; + } + const Alignment* rai = my_ensemble->read_stack[*member_it]; + int my_position = is_to_left? position0 - rai->align_start : rai->align_end - position0; + assert(my_position >= 0); + bool group_exists = false; + for (vector::iterator pos_group_it = position_groups_vec.begin(); pos_group_it != position_groups_vec.end(); ++pos_group_it){ + if (my_position == pos_group_it->group_position){ + group_exists = true; + pos_group_it->AddNew(rai); + break; + } + } + if (not group_exists){ + position_groups_vec.push_back(PositionGroup(rai, my_position)); + } + } + if (position_groups_vec.empty()){ + return -1; + } + vector::iterator best_pos_group_it = position_groups_vec.begin(); + for (vector::iterator pos_group_it = position_groups_vec.begin(); pos_group_it != position_groups_vec.end(); ++pos_group_it){ + if (*pos_group_it > *best_pos_group_it){ + best_pos_group_it = pos_group_it; + } + } + return best_pos_group_it->group_position; } // Hard classify each family using its family responsibility -// Compute family_id_ and family_strand_id_, which are similar to read_id_, strand_id_ for MuitiBook -// family_id_ is obtained from the most responsible hypothesis of the family -// family_id_ stores the hard classification results for "families" (can be non-functional) -// family_id_[i] = -1 means the i-th family is classified as an outlier. -// family_id_[i] = 0 means the i-th family is classified as ref. -// family_id_[i] = 1 means the i-th family is classified as the variant allele 1, and so on. +// Compute read_id_ and strand_id_, which are similar to ApproximateHardClassifierForReads +// read_id_ is obtained from the most responsible hypothesis of the family +// read_id_ stores the hard classification results for "families" (can be non-functional) +// read_id_[i] = -1 means the i-th family is classified as an outlier. +// read_id_[i] = 0 means the i-th family is classified as ref. +// read_id_[i] = 1 means the i-th family is classified as the variant allele 1, and so on. void EnsembleEval::ApproximateHardClassifierForFamilies(){ if(not allele_eval.total_theory.GetIsMolecularTag()){ - cout<<"Warning: Skip EnsembleEval::ApproximateHardClassifierForFamilies() because use_mol_tag is off!"<< endl; + cerr<<"Warning: Skip EnsembleEval::ApproximateHardClassifierForFamilies() because use_mol_tag is off!"<< endl; return; } unsigned int num_families = allele_eval.total_theory.my_eval_families.size(); - int fam_count = 0; - family_id_.assign(num_families, -1); // every family starts with outlier - family_strand_id_.assign(num_families, false); - max_family_responsibility_.assign(num_families, 0.0f); + int position0 = variant->position -1; // variant->position 1-base: vcflib/Variant.h + read_id_.assign(num_families, -1); // every family starts with outlier + strand_id_.assign(num_families, false); + dist_to_left_.assign(num_families, -1); + dist_to_right_.assign(num_families, -1); + alt_fam_indices_.resize(allele_identity_vector.size()); + + for(unsigned int fam_idx = 0; fam_idx < allele_eval.total_theory.my_eval_families.size(); ++fam_idx){ + EvalFamily* my_fam = &(allele_eval.total_theory.my_eval_families[fam_idx]); + // Hard classify the family to allele_assigned (-1 = outlier, 0 = ref, 1 = alt1, etc) + int allele_assigned = my_fam->GetFuncFromValid() ? my_fam->MostResponsible() - 1 : -1; // non-func familiy = outlier + read_id_[fam_idx] = allele_assigned; + strand_id_[fam_idx] = (my_fam->strand_key == 0); + + // Classify as an alt + if (allele_assigned > 0){ + alt_fam_indices_[allele_assigned - 1].push_back(fam_idx); + } - for(vector::iterator fam_it = allele_eval.total_theory.my_eval_families.begin(); fam_it != allele_eval.total_theory.my_eval_families.end(); ++fam_it){ - // don't count the non-functional families - if(fam_it->GetFunctionality()){ - family_id_[fam_count] = fam_it->MostResponsible() - 1; + // Assign position + if (allele_assigned > -1){ + dist_to_left_[fam_idx] = GetConsensusPosition(this, my_fam, true); + dist_to_right_[fam_idx] = GetConsensusPosition(this, my_fam, false); + } + } + + if (DEBUG > 0){ + unsigned int max_num_print_per_allele = 100; + vector > print_families_per_allele(allele_identity_vector.size() + 1); + for (unsigned int i_allele = 0; i_allele < print_families_per_allele.size(); ++i_allele){ + print_families_per_allele[i_allele].reserve(max_num_print_per_allele + 1); + } + for(unsigned int fam_idx = 0; fam_idx < read_id_.size(); ++fam_idx){ + if (read_id_[fam_idx] >= 0){ + if (print_families_per_allele[read_id_[fam_idx]].size() <= max_num_print_per_allele){ + print_families_per_allele[read_id_[fam_idx]].push_back(fam_idx); + } + } + } + cout << "+ Hard-classifying "<< num_families << " families on read stack for the variant ("<< PrintVariant(*variant) << "):" << endl; + for (unsigned int i_allele = 0; i_allele < print_families_per_allele.size(); ++i_allele){ + cout << " - Families that are most responsible for allele " << i_allele <<": "; + for (unsigned int i = 0; i < min((unsigned int) print_families_per_allele[i_allele].size(), max_num_print_per_allele); ++i){ + unsigned int i_fam = print_families_per_allele[i_allele][i]; + cout << allele_eval.total_theory.my_eval_families[i_fam].family_barcode << ", "; + } + if ((unsigned int) print_families_per_allele[i_allele].size() > max_num_print_per_allele) + cout << "......"; + cout << endl; + } + } +} + +void EnsembleEval::DetectPossiblePolyploidyAlleles(const vector& allele_freq_est, const ControlCallAndFilters &my_controls, const vector& variant_specific_params){ + assert(allele_freq_est.size() == allele_identity_vector.size() + 1); + float min_of_min_allele_freq = 1.0f; + is_possible_polyploidy_allele.assign(allele_freq_est.size(), false); + + if (DEBUG){ + cout << "+ Detecting Possible Polyploidy Alleles (PPA):" << endl + << " - estimated_allele_freq = " << PrintIteratorToString(allele_freq_est.begin(), allele_freq_est.end()) << endl; + } + + for (int i_allele = (int) allele_freq_est.size() - 1; i_allele >= 0; --i_allele){ + int i_alt = i_allele - 1; + float my_min_allele_freq = (i_allele == 0)? min_of_min_allele_freq : 0.0f; + if (i_allele > 0){ + my_min_allele_freq = FreqThresholdByType(allele_identity_vector[i_alt], my_controls, variant_specific_params[i_alt]); + min_of_min_allele_freq = min(my_min_allele_freq, min_of_min_allele_freq); + } + is_possible_polyploidy_allele[i_allele] = allele_freq_est[i_allele] > my_min_allele_freq; + + if (DEBUG){ + cout << " - Allele "<< i_allele << (is_possible_polyploidy_allele[i_allele]? " may be" : " is not") << " a PPA: estimated_allele_freq[" << i_allele << "] = " << allele_freq_est[i_allele] << (is_possible_polyploidy_allele[i_allele]? " > " : " <= ") << "min-allele-freq = " << my_min_allele_freq << endl; } - max_family_responsibility_[fam_count] = fam_it->family_responsibility[family_id_[fam_count] + 1]; - family_strand_id_[fam_count] = (fam_it->strand_key == 0); - ++fam_count; } - is_hard_classification_for_families_done_ = true; } int EnsembleEval::DetectBestMultiAllelePair(){ + vector allele_freq_estimation; + return DetectBestMultiAllelePair(allele_freq_estimation); +} + + +int EnsembleEval::DetectBestMultiAllelePair(vector& allele_freq_estimation){ int best_alt_ndx = 0; // forced choice with ref //@TODO: just get the plane off the ground //@TODO: do the top pair by responsibility vector< pair > best_allele_test; - int num_hyp_no_null = allele_eval.total_theory.my_hypotheses[0].responsibility.size()-1; + int num_hyp_no_null = (int) allele_identity_vector.size() + 1; best_allele_test.resize(num_hyp_no_null); // null can never be a winner in "best allele" sweepstakes for (unsigned int i_alt=0; i_alt -1){ - best_allele_test[my_alt].second += max_family_responsibility_[i_family]; + best_allele_test[my_alt].second += allele_eval.total_theory.my_eval_families[i_family].family_responsibility[my_alt + 1]; } // otherwise count for nothing } } // not using molecular tagging else{ - if(not is_hard_classification_for_reads_done_){ + if(read_id_.empty()){ ApproximateHardClassifierForReads(); } // take responsibility for(unsigned int i_read = 0; i_read < read_id_.size(); ++i_read){ int my_alt = read_id_[i_read]; if (my_alt > -1){ - best_allele_test[my_alt].second += allele_eval.total_theory.my_hypotheses[i_read].responsibility[my_alt + 1]; + best_allele_test[my_alt].second += allele_eval.total_theory.my_hypotheses[i_read].weighted_responsibility[my_alt + 1]; } // otherwise count for nothing } } + // calculate allele_freq_estimation + float total_weight = 0.0f; + allele_freq_estimation.assign(num_hyp_no_null, 0.0f); + for(int i = 0; i < num_hyp_no_null; ++i){ + total_weight += best_allele_test[i].second; + } + for(int i = 0; i < num_hyp_no_null; ++i){ + allele_freq_estimation[i] = best_allele_test[i].second / total_weight; + } + + if (DEBUG > 0){ + cout << "+ Detecting the best allele pair for the variant ("<< PrintVariant(*variant) << "):" << endl; + cout << " - allele_freq = " << PrintIteratorToString(allele_freq_estimation.begin(), allele_freq_estimation.end()) << " from semi-hard classification result." << endl; + } + // pick my pair of best alleles sort(best_allele_test.begin(), best_allele_test.end(), compare_best_response); // not-null choices + diploid_choice.assign(2, 0); diploid_choice[0] = best_allele_test[0].first; // index of biggest weight diploid_choice[1] = best_allele_test[1].first; // index of second-biggest weight // problematic cases: @@ -619,57 +909,1469 @@ int EnsembleEval::DetectBestMultiAllelePair(){ // sort as final step to avoid best_alt_ndx reflecting a worse allele sort(diploid_choice.begin(),diploid_choice.end()); // now in increasing allele order as needed - is_detect_best_multi_allele_pair_done_ = true; + if (DEBUG > 0){ + cout << " - The best allele pair = ("<< best_allele_test[0].first<<", "<< best_allele_test[1].first << ")"<< endl << endl; + } + return best_alt_ndx; } +void EnsembleEval::MultiAlleleGenotype(float af_cutoff_rej, float af_cutoff_gt, vector &genotype_component, float >_quality_score, float &reject_status_quality_score, int &quality_type){ + if (diploid_choice.empty()){ + // detect best allele pair if not done yet. + DetectBestMultiAllelePair(); + } + // set diploid in gq_pair + allele_eval.cur_state.cur_posterior.gq_pair.freq_pair = diploid_choice; -void EnsembleEval::ComputePosteriorGenotype(int _alt_allele_index,float local_min_allele_freq, - int &genotype_call, float >_quality_score, float &reject_status_quality_score){ - allele_eval.CallGermline(local_min_allele_freq, genotype_call, gt_quality_score, reject_status_quality_score); + // Call by integral + allele_eval.CallByIntegral(af_cutoff_rej, af_cutoff_gt, genotype_component, gt_quality_score, reject_status_quality_score, quality_type); } -void EnsembleEval::MultiAlleleGenotype(float local_min_allele_freq, vector &genotype_component, float >_quality_score, float &reject_status_quality_score, int max_detail_level){ - // detect best allele hard classify - if(not is_detect_best_multi_allele_pair_done_){ // don't need to do it again if we've already done - DetectBestMultiAllelePair(); // diploid_choice set by posterior responsibility +// Rules of setting the effective min fam size: +// If override requested by HS, then effective_min_family_size = the maximum one among all overrides. +// If not override by HS, effective_min_family_size = min_family_size + +void EnsembleEval::SetEffectiveMinFamilySize(const ExtendParameters& parameters, const vector& variant_specific_params){ + assert(allele_identity_vector.size() > 0); + allele_eval.total_theory.effective_min_family_size = (unsigned int) parameters.tag_trimmer_parameters.min_family_size; + + bool is_override = false; + for (unsigned int i_alt = 0; i_alt < variant_specific_params.size(); ++i_alt){ + if (variant_specific_params[i_alt].min_tag_fam_size_override){ + if (variant_specific_params[i_alt].min_tag_fam_size < 1){ + cerr << "WARNING: Fail to override the parameter min_tag_fam_size by " << variant_specific_params[i_alt].min_tag_fam_size << " < 1." << endl; + continue; + } + if (not is_override){ + // This is the first override. + allele_eval.total_theory.effective_min_family_size = (unsigned int) variant_specific_params[i_alt].min_tag_fam_size; + }else{ + allele_eval.total_theory.effective_min_family_size = max(allele_eval.total_theory.effective_min_family_size, (unsigned int) variant_specific_params[i_alt].min_tag_fam_size); + } + is_override = true; + } + } + if (is_override){ + if (DEBUG){ + cout << "+ Override min_fam_size to " << allele_eval.total_theory.effective_min_family_size << endl; + } + return; + } + // Increase min_fam_size if I found an allele is HP-INDEL. + if (parameters.tag_trimmer_parameters.indel_func_size_offset > 0){ + for (unsigned int i_alt = 0; i_alt < allele_identity_vector.size(); ++i_alt){ + if (allele_identity_vector[i_alt].status.isHPIndel){ + allele_eval.total_theory.effective_min_family_size += (unsigned int) parameters.tag_trimmer_parameters.indel_func_size_offset; + if (DEBUG){ + cout << "+ Found allele "<< i_alt + 1 << " is HP-INDEL: increase min_fam_size from " + << parameters.tag_trimmer_parameters.min_family_size + << " to " << allele_eval.total_theory.effective_min_family_size << endl; + } + return; + } + } + } +} + + + +void EnsembleEval::SetAndPropagateParameters(ExtendParameters* parameters, bool use_molecular_tag, const vector& variant_specific_params){ + allele_eval.my_params = parameters->my_eval_control; + // Set debug level + DEBUG = parameters->program_flow.DEBUG; + allele_eval.DEBUG = parameters->program_flow.DEBUG; + allele_eval.cur_state.SetAndPropagateDebug(parameters->program_flow.DEBUG); + allele_eval.total_theory.DEBUG = parameters->program_flow.DEBUG; + // Set target min-allele-freq for fast scan + allele_eval.cur_state.cur_posterior.ref_vs_all.SetTargetMinAlleleFreq(*parameters, variant_specific_params); + allele_eval.cur_state.cur_posterior.gq_pair.SetTargetMinAlleleFreq(*parameters, variant_specific_params); + // Set molecular tag related parameters + allele_eval.total_theory.SetIsMolecularTag(use_molecular_tag); + SetEffectiveMinFamilySize(*parameters, variant_specific_params); + // only rich_json_diagnostic needs full data + allele_eval.total_theory.preserve_full_data = parameters->program_flow.rich_json_diagnostic; +} + +void EnsembleEval::FlowDisruptivenessInReadLevel(const InputStructures &global_context) +{ + for (unsigned int i_read = 0; i_read < read_stack.size(); ++i_read){ + allele_eval.total_theory.my_hypotheses[i_read].FillInFlowDisruptivenessMatrix(global_context.flow_order_vector.at(read_stack[i_read]->flow_order_index), *(read_stack[i_read])); + } + if (allele_eval.total_theory.GetIsMolecularTag()){ + for (vector::iterator fam_it = allele_eval.total_theory.my_eval_families.begin(); fam_it != allele_eval.total_theory.my_eval_families.end(); ++fam_it){ + fam_it->FillInFlowDisruptivenessMatrix(allele_eval.total_theory.my_hypotheses); + } + } +} + + +// The rule for determining FD in the read stack level is the following. +// Suppose I want to determine the FD between i_hyp and j_hyp. Denote the set reads that support i_hyp by Reads(i_hyp). +// (Case 1): Both hypotheses have reads support, i.e., Reads(i_hyp) and Reads(j_hyp) are both non-empty +// (Case 1.a): I find reads in Reads(i_hyp) and reads in Reads(j_hyp) indicate that the pair (i_hyp, j_hyp) is FD. Then the pair (i_hyp, j_hyp) is FD. +// (Case 1.b): I find reads in Reads(i_hyp) shows (i_hyp, j_hyp) is FD, but no (or too few) reads in Reads(j_hyp) says (i_hyp, j_hyp) is FD. Then (i_hyp, j_hyp) is not FD, (i.e. FD bias) +// (Case 1.c): Reads(i_hyp) and Reads(j_hyp) have no read shows (i_hyp, j_hyp) is FD. Then (i_hyp, j_hyp) is not FD. +// (Case 2): i_hyp has reads support but j_hyp doesn't, i.e., Reads(i_hyp) is non-empty but Reads(j_hyp) is empty +// (Case 2.a): Reads(i_hyp) has reads indicate (i_hyp, j_hyp) is FD. Then (i_hyp, j_hyp) is FD. +// (Case 2.b): Reads(i_hyp) has no read indicate (i_hyp, j_hyp) is FD. Then (i_hyp, j_hyp) is not FD. +// (Case 3): Both i_hyp and j_hyp have no read support. Then the FD between the two hypotheses is indefinite. +// Usually, if any of i_hyp and j_hyp is obtained from the best allele pair, then case 3 should not happen. +// @TODO: Use the transitivity of HP-INDEL to determine the FD of the no coverage alleles. +void EnsembleEval::FlowDisruptivenessInReadStackLevel(float min_ratio_for_fd) +{ + unsigned int num_hyp_not_null = allele_identity_vector.size() + 1; // reference + alternatives + global_flow_disruptive_matrix.assign(num_hyp_not_null, vector(num_hyp_not_null, -1)); // Starts with indefinite + float min_posterior_coverage = 0.9f; + // Aggregating too many super low responsibility may exceed min_posterior_coverage. Use min_resp_cutoff to guard against the problem. + float min_resp_cutoff = 0.1f; + + // postrior_coverage[i_hyp] = posterior coverage for i_hyp. + vector postrior_coverage(num_hyp_not_null, 0.0f); + // posterior_fd_counts[i_hyp][j_hyp][i_type] = posterior counts for Reads(i_hyp) that indicates the FD-type of (i_hyp, j_hyp) is i_type. + vector< vector< vector > > posterior_fd_type_counts(num_hyp_not_null, vector< vector >(num_hyp_not_null, {0.0f, 0.0f, 0.0f})); + + if (DEBUG > 0){ + cout << "- Determine Flow-Disruptiveness (FD) of the variant:" << endl + << " - FD codes: 0 = HP-INDEL, 1 = (not HP-INDEL and not flow-disruptive), 2 = flow-disruptive" << endl + << " - min_ratio_for_fd = "<< min_ratio_for_fd << endl; } - // set diploid in gq_pair - allele_eval.cur_state.cur_posterior.gq_pair.freq_pair = diploid_choice; - // scan gq_pair - allele_eval.cur_state.cur_posterior.gq_pair.DoPosteriorFrequencyScan(allele_eval.total_theory, - allele_eval.cur_state.cur_posterior.clustering, - true, ALL_STRAND_KEY, false, max_detail_level); - - //call-germline - int genotype_call; - allele_eval.CallGermline(local_min_allele_freq, genotype_call, gt_quality_score, reject_status_quality_score); - // set the outputs - // start at "het" by choice - genotype_component[0] = diploid_choice[0]; - genotype_component[1] = diploid_choice[1]; - if(genotype_call == 2){ // hom var - genotype_component[0] = diploid_choice[1]; + + + if (allele_eval.total_theory.GetIsMolecularTag()){ + for (vector::iterator fam_it = allele_eval.total_theory.my_eval_families.begin(); fam_it != allele_eval.total_theory.my_eval_families.end(); ++fam_it){ + if (not fam_it->GetFuncFromValid()){ + continue; + } + for (unsigned int i_hyp = 0; i_hyp < num_hyp_not_null; ++i_hyp){ + float resp = fam_it->family_responsibility[i_hyp + 1]; + if (resp < min_resp_cutoff){ + // I don't count low responsible hypotheses. + continue; + } + postrior_coverage[i_hyp] += resp; + for (unsigned int j_hyp = 0; j_hyp < num_hyp_not_null; ++j_hyp){ + // Note that local_flow_disruptiveness_matrix contains the outlier hypothesis + int fd_type = fam_it->GetFlowDisruptiveness(i_hyp + 1, j_hyp + 1); + if (fd_type >= 0){ + posterior_fd_type_counts[i_hyp][j_hyp][fd_type] += resp; + } + } + } + } + } + else{ + for (vector::iterator read_it = allele_eval.total_theory.my_hypotheses.begin(); read_it != allele_eval.total_theory.my_hypotheses.end(); ++read_it){ + if (not read_it->success){ + continue; + } + for (unsigned int i_hyp = 0; i_hyp < num_hyp_not_null; ++i_hyp){ + float resp = read_it->responsibility[i_hyp + 1]; + if (resp < min_resp_cutoff) + continue; + postrior_coverage[i_hyp] += resp; + for (unsigned int j_hyp = 0; j_hyp < num_hyp_not_null; ++j_hyp){ + // Note that local_flow_disruptiveness_matrix contains the outlier hypothesis + int fd_type = read_it->local_flow_disruptiveness_matrix[i_hyp + 1][j_hyp + 1]; + if (fd_type >= 0){ + posterior_fd_type_counts[i_hyp][j_hyp][fd_type] += resp; + } + } + } + } + } + + for (unsigned int i_hyp = 0; i_hyp < num_hyp_not_null; ++i_hyp){ + global_flow_disruptive_matrix[i_hyp][i_hyp] = 0; + for (unsigned int j_hyp = i_hyp + 1; j_hyp < num_hyp_not_null; ++j_hyp){ + // Is it Case 1? + if (postrior_coverage[i_hyp] >= min_posterior_coverage and postrior_coverage[j_hyp] >= min_posterior_coverage){ + // Check in the order of FD->(not FD and not HPINDEL)->(HPINDEL) + for (int type = 2; type >=0; --type){ + if ( posterior_fd_type_counts[i_hyp][j_hyp][type] / postrior_coverage[i_hyp] >= min_ratio_for_fd + and posterior_fd_type_counts[j_hyp][i_hyp][type] / postrior_coverage[j_hyp] >= min_ratio_for_fd){ + global_flow_disruptive_matrix[i_hyp][j_hyp] = type; + break; + } + } + } + // Is it Case 3? + else if (postrior_coverage[i_hyp] < min_posterior_coverage and postrior_coverage[j_hyp] < min_posterior_coverage){ + // global_flow_disruptive_matrix[j_hyp][i_hyp] = -1; // Indefinite, and it was set by default. + } + // Case 2: one hyp has coverage but the other one doesn't + else{ + int hyp_w_cov = i_hyp; + int hyp_wo_cov = j_hyp; + if (postrior_coverage[j_hyp] > postrior_coverage[i_hyp]){ + hyp_w_cov = j_hyp; + hyp_wo_cov = i_hyp; + } + // Check in the order of (HPINDEL)->(not FD and not HPINDEL)->FD + // I check HPINDEL first because I don't believe in those HP-INDEL reads that support the hyp w/ coverage. + for (int type = 0; type < 3; ++type){ + if ( posterior_fd_type_counts[hyp_w_cov][hyp_wo_cov][type] / postrior_coverage[hyp_w_cov] >= min_ratio_for_fd){ + global_flow_disruptive_matrix[i_hyp][j_hyp] = type; + break; + } + } + } + global_flow_disruptive_matrix[j_hyp][i_hyp] = global_flow_disruptive_matrix[i_hyp][j_hyp]; + } + } + + // Propagate the FD to downstream + variant->info["FDVR"].clear(); + for (unsigned int i_alt = 0; i_alt < allele_identity_vector.size(); ++i_alt){ + allele_identity_vector[i_alt].fd_level_vs_ref = global_flow_disruptive_matrix[0][i_alt + 1]; + /* + // I make an exception according to the reference context! + if (allele_identity_vector[i_alt].status.isHPIndel and global_flow_disruptive_matrix[0][i_alt + 1] != 0){ + if (DEBUG > 0){ + cout << " - Overwrite global_flow_disruptive_matrix[0][" << (i_alt + 1) << "] from " + << global_flow_disruptive_matrix[0][i_alt + 1] << " to 0 using the reference context." << endl; + } + allele_identity_vector[i_alt].fd_level_vs_ref = 0; + global_flow_disruptive_matrix[0][i_alt + 1] = 0; + } + */ + string fdvr = "-1"; + if (global_flow_disruptive_matrix[0][i_alt + 1] == 0){ + fdvr = "0"; + }else if (global_flow_disruptive_matrix[0][i_alt + 1] == 1){ + fdvr = "5"; + }else if (global_flow_disruptive_matrix[0][i_alt + 1] == 2){ + fdvr = "10"; + } + variant->info["FDVR"].push_back(fdvr); + } + + if (DEBUG > 0){ + cout << "- Determine Flow-Disruptiveness (FD) of the variant:" << endl + << " - FD codes: 0 = HP-INDEL, 1 = (not HP-INDEL and not flow-disruptive), 2 = flow-disruptive" << endl + << " - min_ratio_for_fd = "<< min_ratio_for_fd << endl; + for (unsigned int i_hyp = 0; i_hyp < posterior_fd_type_counts.size(); ++i_hyp){ + cout << " - For the reads supporting Allele "<< i_hyp << ": " << "posterior coverage = " << postrior_coverage[i_hyp] << endl; + for (unsigned int j_hyp = 0; j_hyp < posterior_fd_type_counts[i_hyp].size(); ++j_hyp){ + if (j_hyp == i_hyp){ + continue; // trivial case + } + cout << " - vs. Allele " << j_hyp << ": posterior coverage indexed by FD codes = ["; + for (unsigned int fd_idx = 0; fd_idx < posterior_fd_type_counts[i_hyp][j_hyp].size(); ++fd_idx){ + cout << posterior_fd_type_counts[i_hyp][j_hyp][fd_idx] << (fd_idx == posterior_fd_type_counts[i_hyp][j_hyp].size() - 1 ? "": ", "); + } + cout << "]" << endl; + } + } + cout << " - FD matrix ('-': trivial, '/': indefinite)" << endl; + for (unsigned int i_hyp = 0; i_hyp < global_flow_disruptive_matrix.size(); ++i_hyp){ + cout << (i_hyp == 0 ? " = [[" : " ["); + for (unsigned int j_hyp = 0; j_hyp < global_flow_disruptive_matrix[i_hyp].size(); ++j_hyp){ + if (i_hyp == j_hyp){ + cout << "-"; + } + else if (global_flow_disruptive_matrix[i_hyp][j_hyp] > -1){ + cout << global_flow_disruptive_matrix[i_hyp][j_hyp]; + } + else{ + cout << "/"; + } + cout << (j_hyp == global_flow_disruptive_matrix[i_hyp].size() - 1? "]": ", "); + } + cout << (i_hyp == global_flow_disruptive_matrix.size() -1 ? "]": ",") << endl; + } + cout<& variant_specific_params, + float& af_cutoff_rej, float& af_cutoff_gt) +{ + af_cutoff_rej = 1.0f; + // The old fashion scheme: choose the minimum one among all alleles + if (not my_controls.use_fd_param ){ + for (unsigned int allele_idx = 0; allele_idx < allele_identity_vector.size(); ++allele_idx){ + af_cutoff_rej = min(af_cutoff_rej, FreqThresholdByType(allele_identity_vector[allele_idx], my_controls, variant_specific_params[allele_idx])); + } + // The allele-freq-cutoff for calling GT is the same as the allele-freq-cutoff for Rej. + af_cutoff_gt = af_cutoff_rej; + return; + } + + // The new approach: + // af_cutoff_rej is the smallest min-allele-freq from the alleles in "diploid_choice" (aka best allele pair). + // The reason of doing this is because I am not going to call any allele other than diploid_choice. + // Therefore, it makes no sense that I use the min-allele-freq from the allele other than diploid_choice. + for (vector::iterator choice_it = diploid_choice.begin(); choice_it != diploid_choice.end(); ++choice_it){ + if (*choice_it == 0){ + // The reference allele is in best allele pair. + continue; + } + af_cutoff_rej = min(af_cutoff_rej, FreqThresholdByType(allele_identity_vector[*choice_it - 1], my_controls, variant_specific_params[*choice_it - 1])); + } + + // Get af_cutoff_gt + if (diploid_choice[0] == 0 or diploid_choice[1] == 0){ + af_cutoff_gt = af_cutoff_rej; + return; + } + + int fd_diploid_choice = global_flow_disruptive_matrix[diploid_choice[0]][diploid_choice[1]]; + + if (fd_diploid_choice == 2){ + af_cutoff_gt = my_controls.filter_fd_10.min_allele_freq; + } + else if (fd_diploid_choice == 1){ + af_cutoff_gt = my_controls.filter_fd_5.min_allele_freq; + } + else if (fd_diploid_choice == 0){ + af_cutoff_gt = my_controls.filter_fd_0.min_allele_freq; + } + else{ + // indefinite case + af_cutoff_gt = my_controls.filter_fd_0.min_allele_freq; + } +} + +void EnsembleEval::VariantFamilySizeHistogram(){ + const int m = 2; + // Basically, the bins will be (< (mode - m + 1)), .... mode - 1, mode, mode + 1, ..., (>(mode + m - 1)) + const int hist_x_num = 2 * m + 1; + // Make sure ApproximateHardClassification is done. + assert(alt_fam_indices_.size() == allele_identity_vector.size()); + for (unsigned int alt_idx = 0; alt_idx < alt_fam_indices_.size(); ++ alt_idx){ + if (alt_fam_indices_[alt_idx].empty()){ + // FAO = 0 means no histogram. + variant->info["VFSH"].push_back("."); + continue; + } + // Calculate the histogram + map fam_size_hist; + for (unsigned int idx = 0; idx < alt_fam_indices_[alt_idx].size(); ++ idx){ + int fam_size = allele_eval.total_theory.my_eval_families[alt_fam_indices_[alt_idx][idx]].GetValidFamSize(); + ++fam_size_hist[fam_size]; + } + + // Get the mode of the histogram + unsigned int mode_counts = fam_size_hist.begin()->second; + int mode_idx = 0; + int hist_len = 0; + for (map::iterator it = fam_size_hist.begin(); it != fam_size_hist.end(); ++it, ++hist_len){ + if (it->second > mode_counts){ + mode_idx = hist_len; + mode_counts = it->second; + } + } + + // See if I need to bin the begin, end of the histogram, respectively + int x_remaining = hist_x_num - 1; // minus the index mode + int x_start_cutoff = mode_idx; + int x_end_cutoff = mode_idx; + while (x_remaining > 0 and (not (x_start_cutoff == 0 and x_end_cutoff == hist_len - 1))){ + if (x_start_cutoff > 0){ + --x_remaining; + --x_start_cutoff; + } + if (x_end_cutoff < hist_len - 1){ + --x_remaining; + ++x_end_cutoff; + } + } + string hist_text = "("; + map::iterator it_start = fam_size_hist.begin(); + if (x_start_cutoff > 0){ + // bin the begin of the histogram + int fam_counts = 0; + for (int idx = 0; idx <= x_start_cutoff; ++idx, ++it_start){ + fam_counts += it_start->second; + } + --it_start; + hist_text += string("(<") + to_string(it_start->first + 1) + string(",") + to_string(fam_counts) +"),"; + ++it_start; + } + string last_hist_text = ""; + map::iterator it_end = fam_size_hist.end(); + if (x_end_cutoff < hist_len - 1){ + // bin the end of the histogram + int fam_counts = 0; + --it_end; + for (int idx = hist_len - 1; idx >= x_end_cutoff; --idx, --it_end){ + fam_counts += it_end->second; + } + ++it_end; + last_hist_text = string("(>") + to_string(it_end->first - 1) + string(",") + to_string(fam_counts) +"))"; + } + + // No need to bin the rest of the family size + for (map::iterator it = it_start; it != it_end; ++it){ + hist_text += string("(") + to_string(it->first) + string(",") + to_string(it->second) +"),"; + } + + if (last_hist_text.empty()){ + hist_text.back() = ')'; + } + else{ + hist_text += last_hist_text; + } + variant->info["VFSH"].push_back(hist_text); + } +} + + +// Check tag similarity if the molecular alternative coverage <= max_alt_cov and compute TGSM for each alternative allele. +// (Step 1): Determine pairwise family similarity +// I claim family A and family B are similar if +// a) Prefix A is partial similar to prefix B "and" suffix A is partial similar to suffix B (partial similar = allow 1 SNP + small HP-INDEL) +// or b) Prefix A is synchronized with prefix B or suffix A is synchronized with suffix B (synchronized = allow small HP-INDEL) +// The criterion a) is used to deal with PCR error occurs on tag. The criterion b) is used to against family cloning during sample prep. +// Note that pairwise family similarity has NO transitivity. +// (Step 2): Calculate TGSM +// I construct a graph by connecting the families using the relation defined by pairwise family similarity. +// TGSM = (number of families in the graph) - (number of isolated sub-graphs) +void EnsembleEval::CalculateTagSimilarity(const MolecularTagManager& mol_tag_manager, int max_alt_cov, int sample_idx) +{ + tag_similar_counts_.assign(allele_identity_vector.size(), 0); // This is TGSM in the vcf record. + unsigned int prefix_tag_len = mol_tag_manager.GetPrefixTagStruct(sample_idx).size(); + unsigned int suffix_tag_len = mol_tag_manager.GetSuffixTagStruct(sample_idx).size(); + + for (unsigned int allele_idx = 0; allele_idx < alt_fam_indices_.size(); ++allele_idx){ + unsigned int allele_fam_cov = alt_fam_indices_[allele_idx].size(); + if ((int) allele_fam_cov > max_alt_cov or allele_fam_cov == 0){ + continue; + } + // Step 1: Determine pairwise tag similarity + bool non_trivial_similar_tag_found = false; // Do I find any pairwise similar families? + vector > pairwise_tag_similar_matrix; + pairwise_tag_similar_matrix.assign(allele_fam_cov, vector(allele_fam_cov, false)); + for (unsigned int fam_idx_1 = 0; fam_idx_1 < allele_fam_cov; ++fam_idx_1){ + pairwise_tag_similar_matrix[fam_idx_1][fam_idx_1] = true; // pairwise tag similarity is a reflexive relation + const string& tag_1 = allele_eval.total_theory.my_eval_families[alt_fam_indices_[allele_idx][fam_idx_1]].family_barcode; + if (prefix_tag_len + suffix_tag_len != tag_1.size()){ + cerr << "ERROR: The molecular tag "<< tag_1 << " doesn't match the tag structure "<< mol_tag_manager.GetPrefixTagStruct(sample_idx) <<" + "< > subgraph_to_family; + int num_isolated_subgraphs = 0; + if (non_trivial_similar_tag_found){ + FindNodesInIsoSubGraph(pairwise_tag_similar_matrix, subgraph_to_family, true); + num_isolated_subgraphs = (int) subgraph_to_family.size(); + }else{ + num_isolated_subgraphs = (int) allele_fam_cov; + } + tag_similar_counts_[allele_idx] = (int) allele_fam_cov - num_isolated_subgraphs; + + if (DEBUG > 0){ + cout <<"+ Checking tag similarity of the "<< allele_fam_cov <<" families that support allele " << allele_idx + 1 << endl; + for (unsigned int fam_idx = 0; fam_idx < allele_fam_cov; ++fam_idx){ + const string& fam_barcode = allele_eval.total_theory.my_eval_families[alt_fam_indices_[allele_idx][fam_idx]].family_barcode; + cout << " - Family #" << alt_fam_indices_[allele_idx][fam_idx] << " \"" << fam_barcode.substr(0, prefix_tag_len) << "\" + \"" << fam_barcode.substr(prefix_tag_len) << "\" is similar to "; + for (unsigned int fam_idx_2 = 0; fam_idx_2 < allele_fam_cov; ++fam_idx_2){ + if (pairwise_tag_similar_matrix[fam_idx][fam_idx_2] and fam_idx != fam_idx_2){ + const string& sim_fam_barcode = allele_eval.total_theory.my_eval_families[alt_fam_indices_[allele_idx][fam_idx_2]].family_barcode; + cout << "Family #" << alt_fam_indices_[allele_idx][fam_idx_2] << " \"" << sim_fam_barcode.substr(0, prefix_tag_len) << "\" + \"" << sim_fam_barcode.substr(prefix_tag_len) << "\", "; + } + } + cout << endl; + } + if (tag_similar_counts_[allele_idx] > 0){ + cout << "+ Found "<< num_isolated_subgraphs << " isolated subgraph(s) of tag-similar families for allele "<< allele_idx + 1 << ": " << endl; + int group_idx = 0; + for (list >::iterator subgraph_it = subgraph_to_family.begin(); subgraph_it != subgraph_to_family.end(); ++subgraph_it, ++group_idx){ + cout << " - Subgraph "<< group_idx << " consists of " << subgraph_it->size() <<" families: "; + for (list::iterator fam_idx_it = subgraph_it->begin(); fam_idx_it != subgraph_it->end(); ++fam_idx_it){ + cout << "#"<< alt_fam_indices_[allele_idx][*fam_idx_it] << ", "; + } + cout << endl; + } + } + else{ + cout << "+ No tag-similar family found for allele " << allele_idx + 1 << endl; + } + cout << "+ Allele "<< allele_idx + 1 << " has TGSM = " << allele_fam_cov << " - " << num_isolated_subgraphs << " = " << tag_similar_counts_[allele_idx] << endl; + } + } + if (DEBUG > 0) { + cout << endl; + } +} + +bool CompareIntList(const list& list_1, const list& list_2){ + if (list_1.empty()){ + return false; } - else if(genotype_call == 0){ // hom ref - genotype_component[1] = diploid_choice[0]; + else if (list_2.empty()){ + return true; } + return (*(list_1.begin()) < *(list_2.begin())); } +// Consider a non-directed graph of N nodes where the connectivity between node i and j is determined by connectivity_matrix[i][j], i.e., true if connected. +// The function groups the nodes that are transitively connected. +// (Note): connectivity_matrix "must be" symmetric, i.e., connectivity_matrix[i][j] = connectivity_matrix[j][i] +// (Example): N = 5, connectivity_matrix[0][1] = 1, connectivity_matrix[1][2] = 1, connectivity_matrix[3][4] = 1, and all other upper triangle elements are 0. +// Then there are two isolated subgraphs 0-1-2 and 3-4 => subgraph_to_nodes = {{0, 1, 2}, {3, 4}}. +void FindNodesInIsoSubGraph(const vector >& connectivity_matrix, list >& subgraph_to_nodes, bool sort_by_index){ + int num_of_nodes = (int) connectivity_matrix.size(); + int num_nodes_in_subgraphs = 0; // just for safety check + list > dummy_list; + const list >::iterator null_it = dummy_list.begin(); + vector >::iterator> node_to_subgraph(num_of_nodes, null_it); // a look-up table to map from nodes to sub-graph + subgraph_to_nodes.resize(0); + + for (int node_idx_1 = 0; node_idx_1 < num_of_nodes; ++node_idx_1){ + if (node_to_subgraph[node_idx_1] == null_it){ + // This is the first time fam_idx_1 shows up. Create a new subgraph for it. + subgraph_to_nodes.push_back(list(1, node_idx_1)); + node_to_subgraph[node_idx_1] = --(subgraph_to_nodes.end()); + } + list >::iterator& master_subgraph = node_to_subgraph[node_idx_1]; + // Now let the nodes connected to node_idx_1 join the subgraph where node_idx_1 belongs to. + // No need to process node_idx_2 if node_idx_2 < node_idx_1, since it has been carried out previously. + for (int node_idx_2 = node_idx_1 + 1; node_idx_2 < num_of_nodes; ++node_idx_2){ + // Safety check: connectivity_matrix must be symmetric + assert(connectivity_matrix[node_idx_1][node_idx_2] == connectivity_matrix[node_idx_2][node_idx_1]); + if (not connectivity_matrix[node_idx_1][node_idx_2]){ + continue; + } + if (node_to_subgraph[node_idx_2] == null_it){ + node_to_subgraph[node_idx_2] = master_subgraph; + master_subgraph->push_back(node_idx_2); + }else if (node_to_subgraph[node_idx_2] != master_subgraph){ + // store the last node in master_subgraph before merging + list::iterator last_node_in_master_before_merge = --(master_subgraph->end()); + // Let all nodes connected to node_idx_2 merge into master_subgraph + master_subgraph->splice(master_subgraph->end(), *(node_to_subgraph[node_idx_2])); + // Destroy the original subgraph where node_idx_2 belongs to. + subgraph_to_nodes.erase(node_to_subgraph[node_idx_2]); + // Update node_to_subgraph for the nodes that are newly merged into master_subgraph + for (list::iterator node_it = ++last_node_in_master_before_merge; node_it != master_subgraph->end(); ++node_it){ + node_to_subgraph[*node_it] = master_subgraph; + } + } + } + } + + // Sort the nodes and sub-graphs + for (list >::iterator graph_it = subgraph_to_nodes.begin(); graph_it != subgraph_to_nodes.end(); ++graph_it){ + if (sort_by_index){ + graph_it->sort(); + } + num_nodes_in_subgraphs += (int) graph_it->size(); + } + if (sort_by_index){ + subgraph_to_nodes.sort(CompareIntList); + } + // Simple safety check. + assert(num_nodes_in_subgraphs == num_of_nodes + and (int) subgraph_to_nodes.size() <= num_of_nodes + and (subgraph_to_nodes.size() > 0 or num_of_nodes == 0)); +} -/* -// do molecular barcode classification -void HypothesisStack::DoMolecularTagClassification(MolecularTag &mol_tag){ - if(not total_theory.GetIsMolecularTag()){ - cout<<"Warning: Skip molecular tag classification since is_molecular_tag == false."<< endl; +string PrintVariant(const vcf::Variant& variant){ + string spacing = "\t"; + string vcf_variant = variant.sequenceName + spacing + + convertToString(variant.position) + spacing + + variant.id + spacing + + variant.ref + spacing; + for(unsigned int i_alt = 0; i_alt < variant.alt.size(); ++i_alt){ + vcf_variant += variant.alt[i_alt]; + if(i_alt != variant.alt.size() - 1){ + vcf_variant += ","; + } + } + return vcf_variant; +} + +class CompareAllelePositions{ +public: + CompareAllelePositions(vector const* allele_identity_vector = NULL){ + allele_identity_vector_ = allele_identity_vector;}; + ~CompareAllelePositions(){}; + bool operator()(int lhs_allele_idx, int rhs_allele_idx) const { + return allele_identity_vector_->at(lhs_allele_idx).start_variant_window < allele_identity_vector_->at(rhs_allele_idx).start_variant_window; + }; +private: + vector const* allele_identity_vector_ = NULL; +}; + +class CompareAlleleGroups{ +public: + CompareAlleleGroups(vector const* allele_identity_vector = NULL){ + allele_identity_vector_ = allele_identity_vector;}; + ~CompareAlleleGroups(){}; + bool operator()(const list& lhs_group, const list& rhs_group) const { + return allele_identity_vector_->at(*(lhs_group.begin())).start_variant_window < allele_identity_vector_->at(*(rhs_group.begin())).start_variant_window; + }; +private: + vector const* allele_identity_vector_; +}; + + +// Finalize the splitting: +// 1) Sort the alleles and groups +// 2) Further split the groups if the group size is too large. +// 2.a) Stage 1: break the connectivity between long Fake HS and others; break the connectivity between Fake HS and HP-INDEL +// 2.b) Stage 2: break the connectivity between all Fake HS and others +// 2.c) Stage 3: break all connectivity +// 2.d) Stage 4: repeat stage 3 again (in case the alleles from the big group move to a small group and let the small group too big.). +void FinalizeSplitting(vector >& allele_connectivity_matrix, const vector& allele_identity_vector, int max_group_size_allowed, list > &allele_groups){ + int debug = allele_identity_vector[0].DEBUG; + // Used to sort the groups + CompareAlleleGroups compare_groups(&allele_identity_vector); + // Used to sort the alleles in a group + CompareAllelePositions compare_positions(&allele_identity_vector); + unsigned int max_iteration = 4; + unsigned int num_iter = 0; + bool keep_itertate = true; + while (keep_itertate and num_iter < max_iteration){ + if (debug > 0){ + cout <<"+ Final variant splitting. Round "<< (num_iter + 1) << ":" << endl; + } + bool max_group_size_achieved = false; + bool connectivity_changed = false; + for (list >::iterator group_it = allele_groups.begin(); group_it != allele_groups.end(); ++group_it){ + // See if I have a group that contains too many alleles. + if ((int) group_it->size() > max_group_size_allowed){ + max_group_size_achieved = true; + if (debug > 0){ + cout << " - The group "<< PrintIteratorToString(group_it->begin(), group_it->end(), "{", "}", ", ", "alt") + << " contains "<< group_it->size() << " alleles (> max_alt_num = " << max_group_size_allowed <<"): splitting needed." << endl; + } + // I let the alleles in this group be isolated unless the other allele has the same start position. + for (list::iterator idx_it = group_it->begin(); idx_it != group_it->end(); ++idx_it){ + // At the first two rounds (num_iter < 2), I only want to break Fake HS. + if (num_iter < 2 and allele_identity_vector[*idx_it].status.isFakeHsAllele){ + for (unsigned int idx = 0; idx < allele_connectivity_matrix.size(); ++idx){ + if (allele_connectivity_matrix[*idx_it][idx] and allele_identity_vector[*idx_it].start_variant_window != allele_identity_vector[idx].start_variant_window){ + if ( (num_iter == 0 and (allele_identity_vector[idx].status.isHPIndel or allele_identity_vector[*idx_it].ref_length > 10)) + or (num_iter == 1)){ + allele_connectivity_matrix[*idx_it][idx] = false; + allele_connectivity_matrix[idx][*idx_it] = false; + connectivity_changed = true; + if (debug > 0){ + cout << " - Breaking the connectivity between alt"<< *idx_it << ": "<< allele_identity_vector[*idx_it].altAllele << "@[" << allele_identity_vector[*idx_it].position0 << ", " << allele_identity_vector[*idx_it].position0 + allele_identity_vector[*idx_it].ref_length + << ") and alt" << idx << ": " << allele_identity_vector[idx].altAllele << "@[" << allele_identity_vector[idx].position0 << ", " << allele_identity_vector[idx].position0 + allele_identity_vector[idx].ref_length << ")." << endl; + } + } + } + } + } + // Then, I will break all alleles in the group. + else if (num_iter >= 2){ + for (unsigned int idx = 0; idx < allele_connectivity_matrix.size(); ++idx){ + if (allele_connectivity_matrix[*idx_it][idx] and (allele_identity_vector[*idx_it].start_variant_window != allele_identity_vector[idx].start_variant_window)){ + allele_connectivity_matrix[*idx_it][idx] = false; + allele_connectivity_matrix[idx][*idx_it] = false; + connectivity_changed = true; + if (debug > 0){ + cout << " - Breaking the connectivity between alt"<< *idx_it << ": "<< allele_identity_vector[*idx_it].altAllele << "@[" << allele_identity_vector[*idx_it].position0 << ", " << allele_identity_vector[*idx_it].position0 + allele_identity_vector[*idx_it].ref_length + << ") and alt" << idx << ": " << allele_identity_vector[idx].altAllele << "@[" << allele_identity_vector[idx].position0 << ", " << allele_identity_vector[idx].position0 + allele_identity_vector[idx].ref_length << "), "<< endl; + } + } + } + } + } + } + } + + if (max_group_size_achieved){ + // Split the variant using the modified allele_connectivity_matrix. + if (connectivity_changed){ + FindNodesInIsoSubGraph(allele_connectivity_matrix, allele_groups, false); + } + }else{ + keep_itertate = false; + if (debug > 0){ + cout <<" - No splitting needed." << endl; + } + } + ++num_iter; + } + // Must sort the alleles in each group first (because compare_groups will use the pos of the first allele for sorting). + for (list >::iterator group_it = allele_groups.begin(); group_it != allele_groups.end(); ++group_it){ + group_it->sort(compare_positions); + } + // Then sort the groups of alleles. + allele_groups.sort(compare_groups); +} + + +// This function helps the candidate to split the multi-allele variant into smaller variants +void SplitAlleleIdentityVector(const vector& allele_identity_vector, list >& allele_groups, const ReferenceReader &ref_reader, int max_group_size_allowed) +{ + int num_alt = (int) allele_identity_vector.size(); + map, LocalReferenceContext> contex_dict; + vector > allele_connectivity_matrix(num_alt, vector(num_alt)); + + if (num_alt == 1){ + // Don't waste my time on the trivial splitting. + allele_groups = {{0}}; return; } - // Do outlier pre-filtering because we will assume no functional family is an outlier. - float data_reliability = my_params.DataReliability(); - total_theory.OutlierFiltering(data_reliability); - // Do classification - total_theory.MolecularTagClassifier(mol_tag); + // (Step 1): Get the padding removed allele_identity_vector + // Note that allele connectivity should be determined using the "padding removed version" of the alt alleles. + // In particular, I need the splicing window of padding removed allele. + vector padding_removed_allele_identity_vector; + padding_removed_allele_identity_vector.resize(num_alt); + for (int i_alt = 0; i_alt < num_alt; ++i_alt){ + int num_padding = allele_identity_vector[i_alt].num_padding_added.first + allele_identity_vector[i_alt].num_padding_added.second; + if (num_padding == 0 or allele_identity_vector[i_alt].status.isProblematicAllele){ + // Usually, calling this function means that there are padding bases added. Copying the original allele_identity would be rare. + padding_removed_allele_identity_vector[i_alt] = allele_identity_vector[i_alt]; + continue; + } + padding_removed_allele_identity_vector[i_alt].DEBUG = allele_identity_vector[i_alt].DEBUG; + pair< map, LocalReferenceContext>::iterator, bool> context_finder; + // context_finder.first->second is the context for the padding pair allele_identity_vector[i_alt].num_padding_added. + context_finder = contex_dict.insert(pair, LocalReferenceContext> (allele_identity_vector[i_alt].num_padding_added, LocalReferenceContext())); + // context_finder.second indicates did I see this context before? + if (context_finder.second){ + // Didn't see this context before. Need to detect context. + context_finder.first->second.DetectContextAtPosition(ref_reader, + allele_identity_vector[i_alt].chr_idx, allele_identity_vector[i_alt].start_variant_window, + allele_identity_vector[i_alt].ref_length - num_padding); + } + if (not context_finder.first->second.context_detected){ + padding_removed_allele_identity_vector[i_alt].status.isProblematicAllele = true; + continue; + } + padding_removed_allele_identity_vector[i_alt].altAllele = allele_identity_vector[i_alt].altAllele.substr(allele_identity_vector[i_alt].num_padding_added.first, (int) allele_identity_vector[i_alt].altAllele.size() - num_padding); + padding_removed_allele_identity_vector[i_alt].position0 = allele_identity_vector[i_alt].start_variant_window; + padding_removed_allele_identity_vector[i_alt].ref_length = allele_identity_vector[i_alt].ref_length - num_padding; + padding_removed_allele_identity_vector[i_alt].chr_idx = allele_identity_vector[i_alt].chr_idx; + padding_removed_allele_identity_vector[i_alt].status.isFakeHsAllele = allele_identity_vector[i_alt].status.isFakeHsAllele; + if (not padding_removed_allele_identity_vector[i_alt].CharacterizeVariantStatus(context_finder.first->second, ref_reader)){ + padding_removed_allele_identity_vector[i_alt].status.isProblematicAllele = true; + continue; + } + padding_removed_allele_identity_vector[i_alt].CalculateWindowForVariant(context_finder.first->second, ref_reader); + } + + // (Step 2): Determine allele connectivity + for (int i_alt = 0; i_alt < num_alt; ++i_alt){ + allele_connectivity_matrix[i_alt][i_alt] = true; + for (int j_alt = i_alt + 1; j_alt < num_alt; ++j_alt){ + allele_connectivity_matrix[i_alt][j_alt] = IsAllelePairConnected(padding_removed_allele_identity_vector[i_alt], padding_removed_allele_identity_vector[j_alt]); + allele_connectivity_matrix[j_alt][i_alt] = allele_connectivity_matrix[i_alt][j_alt]; + } + } + + // (Step 3): Split the alleles into groups + FindNodesInIsoSubGraph(allele_connectivity_matrix, allele_groups, false); + + // (Step 4): Finalize the groups and alleles: + // (4.a) Sort the alleles and groups + // (4.b) Further split the groups that contain too many alleles. + FinalizeSplitting(allele_connectivity_matrix, padding_removed_allele_identity_vector, max_group_size_allowed, allele_groups); +} + +// Given variant candidates, calculate the end of the look ahead window for candidate generator, +// where look ahead window = [variant_window_end, look_ahead_end). +// I.e., the candidate generator should make sure that there is NO other variant till the (0-based) position at (look_ahead_end_0 - 1), while a variant @ look_ahead_end_0 is fine. +int EnsembleEval::CalculateLookAheadEnd0(const ReferenceReader &ref_reader){ + int chr_size = ref_reader.chr_size(seq_context.chr_idx); // The size of the chromosome. + // (Step 1): At least lookahead to the end of the splicing window to make sure there is no splicing hazard of the current variant interfered the variant at the future position. + int look_ahead_end_0 = multiallele_window_end; + // Can't lookahead beyond the chromosome! + if (look_ahead_end_0 >= chr_size) + return chr_size; + + // (Step 2): Keep looking ahead until this variant will not interfere any variant at the future position. + // I.e., the splicing lower bound at look_ahead_end_0 should >= variant_window_end. + // Note that all windows defined here are left-closed, right-open. + LocalReferenceContext future_context; // The context at the future position + bool keep_lookahead = true; + // I assume that Freebayes doesn't add right anchors to an alt allele for no reason. There must be another alt allele pushes the variant window to the right. (Of course it is not true for HS allele). + // If the assumption doesn't hold, then the look ahead will be more conservative, not hurt. + const int variant_window_end = (int) seq_context.position0 + (int) seq_context.reference_allele.size(); + int splicing_lower_bound_at_look_ahead_end = -1; + bool keep_look_ahead = true; + + if (DEBUG){ + cout << "+ Investigating lookahead window (0-based) for the variant (1-based) (" << PrintVariant(*variant) <<"): "<< endl + << " - Current splicing window end = " << multiallele_window_end << ", which is the lower bound of lookahead window end." << endl + << " - Current variant window end = " << variant_window_end << endl + << " + Finding the smallest future position that won't be interfered by this variant (i.e., splicing lower bound@pos >= current variant window end):" << endl; + } + + while (keep_look_ahead){ + future_context.DetectContextAtPosition(ref_reader, seq_context.chr_idx, look_ahead_end_0, 1); + // Calculate the splicing lower bound at look_ahead_end_0 + splicing_lower_bound_at_look_ahead_end = future_context.SplicingLeftBound(ref_reader); + + if (DEBUG){ + cout << " - pos = " << look_ahead_end_0 << ", Splicing lower bound@pos = " << splicing_lower_bound_at_look_ahead_end << endl; + } + + if (splicing_lower_bound_at_look_ahead_end >= variant_window_end) { + keep_look_ahead = false; + } + else{ + keep_look_ahead = (++look_ahead_end_0 < chr_size); + } + } + + if (DEBUG){ + cout << " - Lookahead window end = " << look_ahead_end_0 + << ", splicing lower bound @(lookahead end) = " << splicing_lower_bound_at_look_ahead_end << endl; + } + return look_ahead_end_0; +} + +// Given the candidate alleles, determine the maximally possible split of the variant (or group of the alternative alleles) that can be correctly (i.e., w/o high FXX) evaluated by the evaluator. +// e.g. output: allele_groups = {{0,1,2}, {3, 4}, {5}}. Then alt[0], alt[1], alt[2] must be evaluated jointly; alt[3], alt[4] must be evaluated jointly; alt[5] can be evaluated individually. +void EnsembleEval::SplitMyAlleleIdentityVector(list >& allele_groups, const ReferenceReader &ref_reader, int max_group_size_allowed){ + SplitAlleleIdentityVector(allele_identity_vector, allele_groups, ref_reader, max_group_size_allowed); + + if (DEBUG){ + cout << "+ Investigating variant splitting for the variant (" << PrintVariant(*variant) <<"): "<< endl + << " - There are " << allele_groups.size() << " groups of alternative alleles identified." << endl; + int group_idx = 0; + for (list >::iterator g_it = allele_groups.begin(); g_it != allele_groups.end(); ++g_it, ++group_idx){ + cout << " - Group #" << group_idx << " consists of "<< g_it->size() << " alternative alleles: " + << PrintIteratorToString(g_it->begin(), g_it->end(), "{", "}", ", ", "alt ") << endl; + } + } +} + +void EnsembleEval::SetupAllAlleles(const ExtendParameters ¶meters, + const InputStructures &global_context, + const ReferenceReader &ref_reader) +{ + seq_context.DetectContext(*variant, global_context.DEBUG, ref_reader); + allele_identity_vector.resize(variant->alt.size()); + + if (global_context.DEBUG > 0 and variant->alt.size() > 0) { + cout << "Investigating variant candidate " << seq_context.reference_allele << " -> " << variant->alt[0]; + for (unsigned int i_allele = 1; i_allele < allele_identity_vector.size(); i_allele++) + cout << ',' << variant->alt[i_allele]; + cout << endl; + } + + // Make sure the vectors are initialized. + assert(variant->alt.size() == variant->alt_orig_padding.size()); + assert(variant->alt.size() == variant->isAltHotspot.size()); + assert(variant->alt.size() == variant->isAltFakeHotspot.size()); + + //now calculate the allele type (SNP/Indel/MNV/HPIndel etc.) and window for hypothesis calculation for each alt allele. + for (unsigned int i_allele = 0; i_allele < allele_identity_vector.size(); i_allele++) { + allele_identity_vector[i_allele].status.isHotSpot = variant->isHotSpot; + allele_identity_vector[i_allele].status.isHotSpotAllele = variant->isAltHotspot[i_allele]; + allele_identity_vector[i_allele].status.isFakeHsAllele = variant->isAltFakeHotspot[i_allele]; + allele_identity_vector[i_allele].filterReasons.clear(); + allele_identity_vector[i_allele].DEBUG = global_context.DEBUG; + + allele_identity_vector[i_allele].indelActAsHPIndel = parameters.my_controls.filter_variant.indel_as_hpindel; + allele_identity_vector[i_allele].getVariantType(variant->alt[i_allele], seq_context, + global_context.ErrorMotifs, parameters.my_controls.filter_variant, ref_reader, variant->alt_orig_padding[i_allele]); + allele_identity_vector[i_allele].CalculateWindowForVariant(seq_context, ref_reader); + } + + //GetMultiAlleleVariantWindow(); + multiallele_window_start = -1; + multiallele_window_end = -1; + + + // Mark Ensemble for realignment if any of the possible variants should be realigned + // TODO: Should we exclude already filtered alleles? + for (unsigned int i_allele = 0; i_allele < allele_identity_vector.size(); i_allele++) { + //if (!allele_identity_vector[i_allele].status.isNoCallVariant) { + if (allele_identity_vector[i_allele].start_splicing_window < multiallele_window_start or multiallele_window_start == -1) + multiallele_window_start = allele_identity_vector[i_allele].start_splicing_window; + if (allele_identity_vector[i_allele].end_splicing_window > multiallele_window_end or multiallele_window_end == -1) + multiallele_window_end = allele_identity_vector[i_allele].end_splicing_window; + + if (allele_identity_vector[i_allele].ActAsSNP() && parameters.my_controls.filter_variant.do_snp_realignment) { + doRealignment = doRealignment or allele_identity_vector[i_allele].status.doRealignment; + } + if (allele_identity_vector[i_allele].ActAsMNP() && parameters.my_controls.filter_variant.do_mnp_realignment) { + doRealignment = doRealignment or allele_identity_vector[i_allele].status.doRealignment; + } + } + // pass allele windows back down the object + for (unsigned int i_allele = 0; i_allele < allele_identity_vector.size(); i_allele++) { + allele_identity_vector[i_allele].multiallele_window_start = multiallele_window_start; + allele_identity_vector[i_allele].multiallele_window_end = multiallele_window_end; + } + + + if (global_context.DEBUG > 0) { + cout << "Realignment for this candidate is turned " << (doRealignment ? "on" : "off") << endl; + cout << "Final window for multi-allele: " << ": (" << multiallele_window_start << ") "; + for (int p_idx = multiallele_window_start; p_idx < multiallele_window_end; p_idx++) + cout << ref_reader.base(seq_context.chr_idx,p_idx); + cout << " (" << multiallele_window_end << ") " << endl; + } +} + + +void EnsembleEval::SpliceAllelesIntoReads(PersistingThreadObjects &thread_objects, const InputStructures &global_context, + const ExtendParameters ¶meters, const ReferenceReader &ref_reader) +{ + bool changed_alignment; + unsigned int num_valid_reads = 0; + unsigned int num_realigned = 0; + int num_hyp_no_null = allele_identity_vector.size()+1; // num alleles +1 for ref + + // generate null+ref+nr.alt hypotheses per read in the case of do_multiallele_eval + allele_eval.total_theory.my_hypotheses.resize(read_stack.size()); + + for (unsigned int i_read = 0; i_read < allele_eval.total_theory.my_hypotheses.size(); i_read++) { + // --- New splicing function --- + allele_eval.total_theory.my_hypotheses[i_read].success = + SpliceVariantHypotheses(*read_stack[i_read], + *this, + seq_context, + thread_objects, + allele_eval.total_theory.my_hypotheses[i_read].splice_start_flow, + allele_eval.total_theory.my_hypotheses[i_read].splice_end_flow, + allele_eval.total_theory.my_hypotheses[i_read].instance_of_read_by_state, + allele_eval.total_theory.my_hypotheses[i_read].same_as_null_hypothesis, + changed_alignment, + global_context, + ref_reader); + + if (allele_eval.total_theory.my_hypotheses[i_read].success){ + num_valid_reads++; + if (changed_alignment) + num_realigned++; + } + + // if we need to compare likelihoods across multiple possibilities + if (num_hyp_no_null > 2) + allele_eval.total_theory.my_hypotheses[i_read].use_correlated_likelihood = false; + } + + // Check how many reads had their alignment modified + std::ostringstream my_info; + my_info.precision(4); + if (doRealignment and num_valid_reads>0){ + float frac_realigned = (float)num_realigned / (float)num_valid_reads; + // And re-do splicing without realignment if we exceed the threshold + if (frac_realigned > parameters.my_controls.filter_variant.realignment_threshold){ + my_info << "SKIPREALIGNx" << frac_realigned; + doRealignment = false; + for (unsigned int i_read = 0; i_read < allele_eval.total_theory.my_hypotheses.size(); i_read++) { + allele_eval.total_theory.my_hypotheses[i_read].success = + SpliceVariantHypotheses(*read_stack[i_read], + *this, + seq_context, + thread_objects, + allele_eval.total_theory.my_hypotheses[i_read].splice_start_flow, + allele_eval.total_theory.my_hypotheses[i_read].splice_end_flow, + allele_eval.total_theory.my_hypotheses[i_read].instance_of_read_by_state, + allele_eval.total_theory.my_hypotheses[i_read].same_as_null_hypothesis, + changed_alignment, + global_context, + ref_reader); + } + } + else { + my_info << "REALIGNEDx" << frac_realigned; + } + info_fields.push_back(my_info.str()); + } + +} + +// Read and process records appropriate for this variant; positions are zero based +void EnsembleEval::StackUpOneVariant(const ExtendParameters ¶meters, const PositionInProgress& bam_position, int sample_index) +{ + + // Initialize random number generator for each stack -> ensure reproducibility + RandSchrange RandGen(parameters.my_controls.RandSeed); + + read_stack.clear(); // reset the stack + read_stack.reserve(parameters.my_controls.downSampleCoverage); + int read_counter = 0; + + for (Alignment* rai = bam_position.begin; rai != bam_position.end; rai = rai->next) { + + // Check global conditions to stop reading in more alignments + if (rai->original_position > multiallele_window_start) + break; + + // filter reads belonging to other samples + if (rai->sample_index != sample_index) + continue; + + if (rai->alignment.Position > multiallele_window_start) + continue; + + if (rai->filtered) + continue; + + if (rai->alignment.GetEndPosition() < multiallele_window_end) + continue; + + // Reservoir Sampling + if (read_stack.size() < (unsigned int)parameters.my_controls.downSampleCoverage) { + read_counter++; + read_stack.push_back(rai); + } else { + read_counter++; + // produces a uniformly distributed test_position between [0, read_counter-1] + unsigned int test_position = ((double)RandGen.Rand() / ((double)RandGen.RandMax + 1.0)) * (double)read_counter; + if (test_position < (unsigned int)parameters.my_controls.downSampleCoverage) + read_stack[test_position] = rai; + } + } +} + +// The class is simply used for random_shuffle +class MyRandSchrange : private RandSchrange{ +public: + MyRandSchrange(int seed = 1) {SetSeed(seed);} ; + int operator()(int upper_lim) {return Rand() % upper_lim;}; // return a random number between 0 and upper_lim-1 +}; + + +// Contains the information I need for downsampling with mol tagging +struct FamInfoForDownSample +{ + MolecularFamily* ptr_fam; // The pointer of the molecular family. + unsigned int num_reads_remaining; // How many reads that are not picked up after down sampling? + FamInfoForDownSample(MolecularFamily* const fam){ + ptr_fam = fam; + num_reads_remaining = ptr_fam->valid_family_members.size(); // Initially, none of the reads is picked up. + }; + bool operator<(const FamInfoForDownSample &rhs) const { return num_reads_remaining > rhs.num_reads_remaining; }; +}; + +// Compare two func families for sorting. +// The family has a read with larger read count wins. +// valid_family_members_sorted must be sorted! +bool CompareFuncFamilies(const FamInfoForDownSample& fam_0, const FamInfoForDownSample& fam_1) +{ + if (fam_0.ptr_fam->valid_family_members[0]->read_count == 1 and fam_1.ptr_fam->valid_family_members[0]->read_count == 1){ + return fam_0.ptr_fam->valid_family_members.size() > fam_1.ptr_fam->valid_family_members.size(); + } + + unsigned int min_size = min(fam_0.ptr_fam->valid_family_members.size(), fam_1.ptr_fam->valid_family_members.size()); + for (unsigned int i_read = 0; i_read < min_size; ++i_read){ + if (fam_0.ptr_fam->valid_family_members[i_read]->read_count > fam_1.ptr_fam->valid_family_members[i_read]->read_count){ + return true; + } + else if (fam_0.ptr_fam->valid_family_members[i_read]->read_count < fam_1.ptr_fam->valid_family_members[i_read]->read_count){ + return false; + } + } + return fam_0.ptr_fam->GetValidFamSize() > fam_1.ptr_fam->GetValidFamSize(); +} + +// I apply a strategic downsampling algorithm for molecular tagging using the following rules. +// Rule 0: Only reads in functional families will be evaluated. +// Rule 1: Get as many functional families as possible after down sampling +// Rule 2: If no consensus read, prefer to pick up "rich" families. +// Rule 3: For consensus reads, maximize the family size after downsampling. +// I only pick up the reads from my_molecular_families[strand_key] +// num_reads_available: total number of reads in the functional families (from valid_family_members) on the strand specified by strand_key +// num_func_fam: total number of functional families (from valid_family_members) on the strand specified by strand_key +void EnsembleEval::DoDownSamplingMolTag(const ExtendParameters ¶meters, unsigned int effective_min_fam_size, vector< vector > &my_molecular_families, + unsigned int num_reads_available, unsigned int num_func_fam, int strand_key) +{ + MyRandSchrange my_rand_schrange(parameters.my_controls.RandSeed); // The random number generator that we use to guarantee reproducibility. + unsigned int read_counter = 0; // Number of reads on read stack + unsigned int downSampleCoverage = (unsigned int) parameters.my_controls.downSampleCoverage; + + read_stack.clear(); // reset the stack + allele_eval.total_theory.my_eval_families.clear(); + + // (Case 1): I can keep all the reads in all functional families :D + if (num_reads_available <= downSampleCoverage){ + allele_eval.total_theory.my_eval_families.reserve(num_func_fam); + read_stack.reserve(num_reads_available); + + for (vector::iterator family_it = my_molecular_families[strand_key].begin(); + family_it != my_molecular_families[strand_key].end(); ++family_it){ + if (family_it->SetFuncFromValid(effective_min_fam_size)){ + allele_eval.total_theory.my_eval_families.push_back(EvalFamily(family_it->family_barcode, family_it->strand_key, &read_stack)); + for (vector::iterator read_it = family_it->valid_family_members.begin(); read_it != family_it->valid_family_members.end(); ++read_it){ + read_stack.push_back(*read_it); + allele_eval.total_theory.my_eval_families.back().AddNewMember(read_counter); + ++read_counter; + } + } + } + + if (DEBUG > 0){ + cout << endl <<"+ Down sample for molecular tags: "<< endl; + cout << " - Down sample " << num_reads_available << " reads to " << downSampleCoverage << ". "; + cout << "Number of families after down sampling = "<< num_func_fam << "." << endl; + cout << " - Total reads after down sampling = " << read_counter << endl; + } + return; + } + + // (Case 2): I can't preserve all reads but I can preserve all functional families. + // Step 1: Find and sort all available func families + unsigned int reads_remaining = downSampleCoverage; + unsigned int num_of_func_fam_after_down_sampling = 0; + vector func_families; + func_families.reserve(num_func_fam); + for (vector::iterator family_it = my_molecular_families[strand_key].begin(); + family_it != my_molecular_families[strand_key].end(); ++family_it){ + if (family_it->SetFuncFromValid(effective_min_fam_size)){ + func_families.push_back(FamInfoForDownSample(&(*family_it))); + // Always make sure valid_family_members is sorted + if (not family_it->is_valid_family_members_sorted){ + family_it->SortValidFamilyMembers(); + } + } + } + // Random shuffle func_families to get randomness for the tie situation during sorting. + random_shuffle(func_families.begin(), func_families.end(), my_rand_schrange); + // The most important step. Sort func_families according to CompareFuncFamilies + sort(func_families.begin(), func_families.end(), CompareFuncFamilies); + + // Step 2: + // In each family, pick valid_family_members[0] if the read can make the family functional. I call such a read "super read". + // Again, valid_family_members must be sorted, so is func_families. + vector::iterator first_poor_fam_it = func_families.end(); // A family is "poor" if it doesn't have such a "super read". first_poor_fam_it is the first poor family. + for (vector::iterator func_fam_it = func_families.begin(); func_fam_it != func_families.end(); ++func_fam_it){ + if (reads_remaining == 0){ + break; + } + if (func_fam_it->ptr_fam->valid_family_members[0]->read_count >= (int) effective_min_fam_size){ + ++num_of_func_fam_after_down_sampling; + --(func_fam_it->num_reads_remaining); + --reads_remaining; + } + else{ + first_poor_fam_it = func_fam_it; + break; + } + } + + // Step 3: + // Try to let as many poor families be functional as possible, if I still have reads remaining. + vector::iterator last_poor_fam = func_families.end(); + if (reads_remaining > 0){ + for (vector::iterator func_fam_it = first_poor_fam_it; func_fam_it != func_families.end(); ++func_fam_it){ + if (reads_remaining < effective_min_fam_size){ // The simple criterion is not the best implementation because I may lose some func fam. But it should be acceptable. + last_poor_fam = func_fam_it; + break; + } + int down_sampled_fam_size = 0; + for (vector::iterator read_it = func_fam_it->ptr_fam->valid_family_members.begin(); read_it != func_fam_it->ptr_fam->valid_family_members.end(); ++read_it){ + if (reads_remaining == 0 or down_sampled_fam_size >= (int) effective_min_fam_size){ + break; + } + down_sampled_fam_size += ((*read_it)->read_count); + --(func_fam_it->num_reads_remaining); + --reads_remaining; + } + if (down_sampled_fam_size >= (int) effective_min_fam_size){ + ++num_of_func_fam_after_down_sampling; + } + else{ // shouldn't happen + last_poor_fam = func_fam_it; + break; + } + } + } + + // Step 4: + // Pick one read in one family until I don't have read remaining. + // This step should not affect the number of functional families after down sampling. + if (reads_remaining > 0){ + sort(func_families.begin(), last_poor_fam); // Sort by the num_reads_remaining (more num_reads_remaining first). Otherwise it can be super slow in the worst case. + } + while (reads_remaining > 0 and func_families[0].num_reads_remaining > 0){ // Although the criterion (func_families[0].num_reads_remaining > 0) should be redundant, it's always safe to check this here. + for (vector::iterator func_fam_it = func_families.begin(); func_fam_it != last_poor_fam; ++func_fam_it){ + if (reads_remaining == 0 or func_fam_it->num_reads_remaining == 0){ + break; + } + if (func_fam_it->num_reads_remaining > 0){ + --(func_fam_it->num_reads_remaining); + --reads_remaining; + } + } + } + + // Step 5: + // Fill the reads into read_stack + allele_eval.total_theory.my_eval_families.reserve(num_of_func_fam_after_down_sampling); + read_stack.reserve(downSampleCoverage); + + for (vector::iterator func_fam_it = func_families.begin(); func_fam_it != func_families.end(); ++func_fam_it){ + int num_reads_picked = (int) (func_fam_it->ptr_fam->valid_family_members.size()) - func_fam_it->num_reads_remaining; + if (num_reads_picked == 0){ + continue; + } + allele_eval.total_theory.my_eval_families.push_back(EvalFamily(func_fam_it->ptr_fam->family_barcode, func_fam_it->ptr_fam->strand_key, &read_stack)); + allele_eval.total_theory.my_eval_families.back().all_family_members.reserve(num_reads_picked); + + // For fairness, I need to randomly pick up the reads with the same read_count if needed. + if (func_fam_it->num_reads_remaining > 0){ + vector::iterator first_read_it_with_smallest_read_count; + vector::iterator next_read_it_not_with_smallest_read_count; + + if (func_fam_it->ptr_fam->valid_family_members[0]->read_count == func_fam_it->ptr_fam->valid_family_members.back()->read_count){ + // All reads have equal read_count. Of course I need to randomly pick up num_reads_picked reads. + first_read_it_with_smallest_read_count = func_fam_it->ptr_fam->valid_family_members.begin(); + next_read_it_not_with_smallest_read_count = func_fam_it->ptr_fam->valid_family_members.end(); + } + else{ + // The reads in the family have variable read_count. + // If more than one read have smallest_read_count, I need to random shuffle these reads and then do down sampling. + vector::iterator read_it_with_smallest_read_count = func_fam_it->ptr_fam->valid_family_members.begin() + (num_reads_picked - 1); + int smallest_read_count = (*read_it_with_smallest_read_count)->read_count; + // Find the first read after read_it_with_smallest_read_count whose read_count != smallest_read_count + for (next_read_it_not_with_smallest_read_count = read_it_with_smallest_read_count + 1; next_read_it_not_with_smallest_read_count != func_fam_it->ptr_fam->valid_family_members.end(); ++next_read_it_not_with_smallest_read_count){ + if ((*next_read_it_not_with_smallest_read_count)->read_count != smallest_read_count) + break; + } + // Find the last read before read_it_with_smallest_read_count whose read_count != smallest_read_count + if ( (*(func_fam_it->ptr_fam->valid_family_members.begin()))->read_count == smallest_read_count){ + first_read_it_with_smallest_read_count = func_fam_it->ptr_fam->valid_family_members.begin(); + } + else{ + for (first_read_it_with_smallest_read_count = read_it_with_smallest_read_count - 1; first_read_it_with_smallest_read_count != func_fam_it->ptr_fam->valid_family_members.begin(); --first_read_it_with_smallest_read_count){ + if ((*first_read_it_with_smallest_read_count)->read_count != smallest_read_count){ + break; + } + } + ++first_read_it_with_smallest_read_count; + } + } + if (first_read_it_with_smallest_read_count != (next_read_it_not_with_smallest_read_count - 1)){ + // Partially random shuffle the reads in the family. + random_shuffle(first_read_it_with_smallest_read_count, next_read_it_not_with_smallest_read_count, my_rand_schrange); + } + } + // Pickup the first num_reads_picked reads from valid_family_members. + for (int i_read = 0; i_read < num_reads_picked; ++i_read){ + // Add the read into read_stack + read_stack.push_back(func_fam_it->ptr_fam->valid_family_members[i_read]); + // Add the read in to the family + allele_eval.total_theory.my_eval_families.back().AddNewMember(read_counter); + ++read_counter; + } + } + + if (DEBUG > 0){ + cout << "+ Down sample with molecular tagging: "<< endl; + cout << " - Down sample " << num_reads_available << " reads to " << downSampleCoverage << ". " << endl; + cout << " - Number of functional families after down sampling = "<< num_of_func_fam_after_down_sampling<< "." << endl; + cout << " - Total reads after down sampling = " << read_counter << endl; + } +} + +// Currently only take the reads on one strand +void EnsembleEval::StackUpOneVariantMolTag(const ExtendParameters ¶meters, vector< vector > &my_molecular_families, int sample_index) +{ + int strand_key = -1; + unsigned int effective_min_fam_size = allele_eval.total_theory.effective_min_family_size; + vector num_func_fam_by_strand = {0, 0}; + vector num_reads_available_by_strand = {0, 0}; // Here, one consensus read counts one read! + vector num_reads_conuts_available_by_strand = {0, 0}; // Here, one consensus read counts by its read counts! + + assert(allele_eval.total_theory.effective_min_family_size > 0); + + for (unsigned int i_strand = 0; i_strand < my_molecular_families.size(); ++i_strand){ + for (vector< MolecularFamily>::iterator family_it = my_molecular_families[i_strand].begin(); + family_it != my_molecular_families[i_strand].end(); ++family_it){ + // Skip the family if it is not functional + if (not family_it->SetFuncFromAll(effective_min_fam_size)){ + continue; + } + family_it->ResetValidFamilyMembers(); + if (not family_it->is_all_family_members_sorted){ + family_it->SortAllFamilyMembers(); // In case I haven't done this before. + } + + // Apply more filtering criteria to filter out some reads in all_family_members + // The reads in valid_family_members are available for downsampling and get into read stack + for (vector::iterator member_it = family_it->all_family_members.begin(); member_it != family_it->all_family_members.end(); ++member_it){ + // Although it has been done previously, do it again to make sure everything is right. + if ((*member_it)->filtered){ + continue; + } + // Although it has been done previously, do it again to make sure everything is right. + if ((*member_it)->sample_index != sample_index) { + continue; + } + + // Check global conditions to stop reading in more alignments + if ((*member_it)->original_position > multiallele_window_start + or (*member_it)->alignment.Position > multiallele_window_start + or (*member_it)->alignment.GetEndPosition() < multiallele_window_end){ + continue; + } + + // family_members_temp stores the reads which are not filtered out here + family_it->valid_family_members.push_back((*member_it)); + } + family_it->is_valid_family_members_sorted = true; // valid_family_members is of course sorted as well since all_family_members is sorted. + family_it->CountFamSizeFromValid(); + // Determine the functionality from valid_family_members + if (family_it->SetFuncFromValid(effective_min_fam_size)){ + // Count how many reads and functional families available for down sampling + num_reads_available_by_strand[i_strand] += family_it->valid_family_members.size(); + num_reads_conuts_available_by_strand[i_strand] += family_it->GetValidFamSize(); + ++num_func_fam_by_strand[i_strand]; + } + } + } + + // For the current molecular barcoding scheme (bcprimer), the reads in each amplicom should be on on strand only. + // However, we sometimes get families on bo th strands, primarily due to false priming. + // Here I pick the strand that has more functional families + strand_key = num_func_fam_by_strand[0] > num_func_fam_by_strand[1] ? 0 : 1; + + if (DEBUG > 0){ + string which_strand = (strand_key == 0)? "FWD" : "REV"; + cout << endl << "+ Stack up one variant with molecular tagging" << endl + << " - Effective-min-fam-size = " << effective_min_fam_size < fam_size_hist; + for (vector< MolecularFamily>::iterator family_it = my_molecular_families[i_strand].begin(); family_it != my_molecular_families[i_strand].end(); ++family_it){ + int fam_size = family_it->GetFuncFromAll()? family_it->GetValidFamSize() : family_it->GetFamSize(); + ++fam_size_hist[fam_size]; + } + cout << " - "<< (strand_key == 0 ? "FWD" : "REV") << ": fam_size_hist = ["; + for (map::iterator hist_it = fam_size_hist.begin(); hist_it != fam_size_hist.end(); ++hist_it){ + cout << "(" << hist_it->first << ", " << hist_it->second <<"), "; + } + cout << "]" << endl; + } + } + + // Do down-sampling + DoDownSamplingMolTag(parameters, effective_min_fam_size, my_molecular_families, num_reads_available_by_strand[strand_key], num_func_fam_by_strand[strand_key], strand_key); +} + +// ------------------------------------------------------------ + +void EnsembleEval::FilterAllAlleles(const ControlCallAndFilters& my_controls, const vector& variant_specific_params) { + if (seq_context.context_detected) { + for (unsigned int i_allele = 0; i_allele < allele_identity_vector.size(); i_allele++) { + allele_identity_vector[i_allele].DetectCasesToForceNoCall(seq_context, my_controls, variant_specific_params[i_allele]); + } + } +} + + +BasicFilters const * ServeBasicFilterByType(const AlleleIdentity &variant_identity, const ControlCallAndFilters &my_controls){ + // The old logic for serving min-allele-freq based on allele type. Maybe deprecated in the future. + if (not my_controls.use_fd_param){ + if (variant_identity.status.isHotSpot and (not my_controls.hotspots_as_de_novo)) + return &(my_controls.filter_hotspot); + if (variant_identity.ActAsSNP()) + return &(my_controls.filter_snp); + if (variant_identity.ActAsMNP()) + return &(my_controls.filter_mnp); + if (variant_identity.ActAsHPIndel()) + return &(my_controls.filter_hp_indel); + return &(my_controls.filter_snp); + } + + // The new logic for serving min-allele-freq based on fd + if (variant_identity.status.isHotSpotAllele and (not my_controls.hotspots_as_de_novo)) + return &(my_controls.filter_hotspot); + if (variant_identity.fd_level_vs_ref == 0) + return &(my_controls.filter_fd_0); + if (variant_identity.fd_level_vs_ref == 1) + return &(my_controls.filter_fd_5); + if (variant_identity.fd_level_vs_ref == 2) + return &(my_controls.filter_fd_10); + // If indefinate, use the fd-0-min-allele-freq (usually the most conservative one) + return &(my_controls.filter_fd_0); +} + +float FreqThresholdByType(const AlleleIdentity& variant_identity, + const ControlCallAndFilters& my_controls, + const VariantSpecificParams& variant_specific_params) +{ + // Override has the top prioirty + if (variant_specific_params.min_allele_freq_override) + return variant_specific_params.min_allele_freq; + + return ServeBasicFilterByType(variant_identity, my_controls)->min_allele_freq; +} + + +void EnsembleEval::GatherInfoForOfflineFiltering(const ControlCallAndFilters &my_controls, int best_allele_index){ + variant->info["PARAM"].clear(); + variant->info["FDPARAM"].clear(); + variant->info["BAP"].clear(); + variant->info["BAI"].clear(); + variant->info["FDBAP"].clear(); + variant->info["AAHPINDEL"].clear(); + variant->info["ISHPINDEL"].clear(); + + + for (unsigned int i_alt = 0; i_alt < allele_identity_vector.size(); ++i_alt){ + variant->info["AAHPINDEL"].push_back((allele_identity_vector[i_alt].ActAsHPIndel()? "1" : "0")); + variant->info["ISHPINDEL"].push_back((allele_identity_vector[i_alt].status.isHPIndel? "1" : "0")); + } + ControlCallAndFilters my_controls_temp = my_controls; + for (int use_fd = 0; use_fd < 2; ++use_fd){ + my_controls_temp.use_fd_param = (use_fd == 1); + for (unsigned int i_alt = 0; i_alt < allele_identity_vector.size(); ++i_alt){ + BasicFilters const *my_basic_filter = ServeBasicFilterByType(allele_identity_vector[i_alt], my_controls_temp); + string param_type; + if (my_basic_filter == &(my_controls_temp.filter_fd_0)){ + param_type = "fd_0"; + }else if (my_basic_filter == &(my_controls_temp.filter_fd_5)){ + param_type = "fd_5"; + }else if (my_basic_filter == &(my_controls_temp.filter_fd_10)){ + param_type = "fd_10"; + }else if (my_basic_filter == &(my_controls_temp.filter_snp)){ + param_type = "snp"; + }else if (my_basic_filter == &(my_controls_temp.filter_mnp)){ + param_type = "mnp"; + }else if (my_basic_filter == &(my_controls_temp.filter_hp_indel)){ + param_type = "indel"; + }else if (my_basic_filter == &(my_controls_temp.filter_hotspot)){ + param_type = "hotspot"; + }else{ + param_type = "."; + } + variant->info[(my_controls_temp.use_fd_param? "FDPARAM" : "PARAM")].push_back(param_type); + } + } + + // The Best Alt allele Index. Allele X = Alt X - 1 => (X - 1) + variant->info["BAI"].push_back(convertToString(best_allele_index)); + // Best Allele Pair + variant->info["BAP"].push_back(convertToString(diploid_choice[0])); + variant->info["BAP"].push_back(convertToString(diploid_choice[1])); + // FD between Best Allele Pair + string fd_bpa_string; + int fd_bpa = global_flow_disruptive_matrix[diploid_choice[0]][diploid_choice[1]]; + if (fd_bpa == 0){ + fd_bpa_string = "0"; + }else if (fd_bpa == 1){ + fd_bpa_string = "5"; + }else if (fd_bpa == 2){ + fd_bpa_string = "10"; + }else{ + fd_bpa_string = "-1"; + } + variant->info["FDBAP"].push_back(fd_bpa_string); } -*/ diff --git a/Analysis/VariantCaller/EnsembleEval/StackEngine.h b/Analysis/VariantCaller/EnsembleEval/StackEngine.h index 078f8467..53cf7c17 100644 --- a/Analysis/VariantCaller/EnsembleEval/StackEngine.h +++ b/Analysis/VariantCaller/EnsembleEval/StackEngine.h @@ -12,6 +12,7 @@ #include #include #include +#include #include "ExtendedReadInfo.h" #include "CrossHypotheses.h" @@ -47,17 +48,22 @@ class LatentSlate{ vector ll_at_stage; vector start_freq_of_winner; + int DEBUG; + void FastExecuteInference(ShortStack &total_theory, bool update_frequency, bool update_sigma, vector &start_frequency); void LocalExecuteInference(ShortStack &total_theory, bool update_frequency, bool update_sigma, vector &start_frequency); void FastStep(ShortStack &total_theory, bool update_frequency, bool update_sigma); void DetailedStep(ShortStack &total_theory, bool update_frequency, bool update_sigma); - void ScanStrandPosterior(ShortStack &total_theory, bool vs_ref, int max_detail_level); + void ScanStrandPosterior(ShortStack &total_theory, bool vs_ref); void ResetToOrigin(); void PropagateTuningParameters(EnsembleEvalTuningParameters &my_params, int num_hyp_no_null); - LatentSlate(){ + void SetAndPropagateDebug(int debug); + LatentSlate(int debug = 0){ max_iterations = 10; detailed_integral = true; iter_done = 0; + DEBUG = debug; + SetAndPropagateDebug(DEBUG); }; }; @@ -68,6 +74,8 @@ class HypothesisStack{ ShortStack total_theory; EnsembleEvalTuningParameters my_params; bool try_alternatives; + int DEBUG; + vcf::Variant* variant; // just for debug message. vector ll_record; vector > try_hyp_freq; @@ -84,12 +92,12 @@ class HypothesisStack{ void SetAlternateFromMain(); void ExecuteExtremeInferences(); void TriangulateRestart(); - float ExecuteOneRestart(vector &restart_hyp, int max_detail_level = 0); + float ExecuteOneRestart(vector &restart_hyp); void ExecuteInference(); - void InitForInference(PersistingThreadObjects &thread_objects, vector& read_stack, const InputStructures &global_context, int num_hyp_no_null, vector &allele_identity_vector); + void InitForInference(PersistingThreadObjects &thread_objects, vector& read_stack, const InputStructures &global_context, vector &allele_identity_vector); // tool for posterior density estimation - bool CallGermline(float hom_safety, int &genotype_call, float &quasi_phred_quality_score, float &reject_status_quality_score); + bool CallByIntegral(float af_cutoff_rej, float af_cutoff_gt, vector &genotype_component, float &quasi_phred_quality_score, float &reject_status_quality_score, int &qual_type); float ReturnMaxLL(); }; @@ -108,67 +116,117 @@ class EnsembleEval { int multiallele_window_end; vector info_fields; bool doRealignment; + int DEBUG; // Allele evaluation information HypothesisStack allele_eval; vector diploid_choice; + vector is_possible_polyploidy_allele; + //@TODO: Use enumerate to represent the fd-code + vector > global_flow_disruptive_matrix; EnsembleEval(vcf::Variant &candidate_variant) { - diploid_choice.assign(2,0); - diploid_choice[1]=1; // ref = 0, alt = 1 + diploid_choice.clear(); variant = &candidate_variant; + allele_eval.variant = variant; info_fields.clear(); multiallele_window_start = -1; multiallele_window_end = -1; doRealignment = false; - is_detect_best_multi_allele_pair_done_ = false; - is_hard_classification_for_reads_done_ = false; - is_hard_classification_for_families_done_ = false; + DEBUG = 0; + read_id_.clear(); + strand_id_.clear(); + dist_to_left_.clear(); + dist_to_right_.clear(); }; - //! @brief Create a detailed picture about this variant and all its alleles + //! @brief Set the parameters of the evaluator + void SetAndPropagateParameters(ExtendParameters* parameters, bool use_molecular_tag, const vector& variant_specific_params); + //! @brief Generate the base space hypotheses for each read + void SpliceAllelesIntoReads(PersistingThreadObjects &thread_objects, const InputStructures &global_context, + const ExtendParameters ¶meters, const ReferenceReader &ref_reader); + //! @brief Get MLLD from the evaluator + void ScanSupportingEvidence(float &mean_ll_delta, int i_allele); + //! @brief Calculate multi-min-allele-freq + void MultiMinAlleleFreq(const vector& multi_min_allele_freq); + //! @brief Output additional information in the INFO field to make off-line filter possible + void GatherInfoForOfflineFiltering(const ControlCallAndFilters &my_controls, int best_allele_index); + + //------------------------------------------------------------------ + // Functions for molecular tagging + //! @brief Set the min family size that will be used in the evaluator. + void SetEffectiveMinFamilySize(const ExtendParameters& parameters, const vector& variant_specific_params); + //! @brief Calculate the tag similarity for molecular tagging + void CalculateTagSimilarity(const MolecularTagManager& mol_tag_manager, int max_alt_cov, int sample_idx); + //! @brief Calculate the variant family histogram + void VariantFamilySizeHistogram(); + //------------------------------------------------------------------ + // Functions of allele related (not go to the reads) are defined here + //! @brief Setup the alleles, i.e., context investigation, allele classification, etc. void SetupAllAlleles(const ExtendParameters ¶meters, const InputStructures &global_context, - const ReferenceReader &ref_reader, int chr_idx); - void FilterAllAlleles(const ClassifyFilters &filter_variant, const vector& variant_specific_params); + const ReferenceReader &ref_reader); + //! @brief Filter out undesired alleles + void FilterAllAlleles(const ControlCallAndFilters& my_controls, const vector& variant_specific_params); + //! @brief Calculate the end of the look ahead window (primarily for candidate generator) + int CalculateLookAheadEnd0(const ReferenceReader &ref_reader); + //! @brief Split the current variant into as many callable smaller variants as possible (primarily for candidate generator)) + void SplitMyAlleleIdentityVector(list >& allele_group, const ReferenceReader &ref_reader, int max_group_size_allowed); + //------------------------------------------------------------------ + // Functions for filling read stack are defined here + //! @brief Fill the read stack w/o molecular tagging void StackUpOneVariant(const ExtendParameters ¶meters, const PositionInProgress& bam_position, int sample_index); - void StackUpOneVariantMolTag(const ExtendParameters ¶meters, vector< vector< MolecularFamily > > &my_molecular_families_one_strand, int sample_index); - void DoDownSamplingMolTag(const ExtendParameters ¶meters, vector< vector< MolecularFamily > > &my_molecular_families, + //! @brief Fill the read stack w/ molecular tagging + void StackUpOneVariantMolTag(const ExtendParameters ¶meters, vector< vector< MolecularFamily> > &my_molecular_families_one_strand, int sample_index); + //! @brief Strategic downsampling algorithm for molecular tagging + void DoDownSamplingMolTag(const ExtendParameters ¶meters, unsigned int effective_min_fam_size, vector< vector< MolecularFamily> > &my_molecular_families, unsigned int num_reads_available, unsigned int num_func_fam, int strand_key); - void SpliceAllelesIntoReads(PersistingThreadObjects &thread_objects, const InputStructures &global_context, - const ExtendParameters ¶meters, const ReferenceReader &ref_reader, int chr_idx); + //------------------------------------------------------------------ + // Functions of hard classification of reads/families are defined here + //! @brief Hard classification of reads void ApproximateHardClassifierForReads(); - void ApproximateHardClassifierForFamilies(); // calculate the family id for cfDNA - void ScanSupportingEvidence(float &mean_ll_delta, int i_allele); + //!@ brief Hard classification of families for molecular tagging + void ApproximateHardClassifierForFamilies(); // calculate the family id for mol taging + //------------------------------------------------------------------ + // Functions for high level evaluation results (e.g. QUAL, GT, GQ) are defined here + //! @brief Determine the best two alleles int DetectBestMultiAllelePair(); - void ComputePosteriorGenotype(int _alt_allele_index,float local_min_allele_freq, int &genotype_call, - float >_quality_score, float &reject_status_quality_score); - void MultiAlleleGenotype(float local_min_allele_freq, vector &genotype_component, - float >_quality_score, float &reject_status_quality_score, - int max_detail_level = 0); + //! @brief Determine the best two alleles and output the allele_freq_estimation in the detection of best allele pair + int DetectBestMultiAllelePair(vector& allele_freq_estimation); + //! @brief Determine possible polyploidy alleles + void DetectPossiblePolyploidyAlleles(const vector& allele_freq, const ControlCallAndFilters &my_controls, const vector& variant_specific_params); + //! @brief Get allele frequency cutoff according to the level of FD or allele type + void ServeAfCutoff(const ControlCallAndFilters &my_controls, const vector& variant_specific_params, + float& af_cutoff_rej, float& af_cutoff_gt); + //! @brief Calculate QUAL, GT, GQ + void MultiAlleleGenotype(float af_cutoff_rej, float af_cutoff_gt, vector &genotype_component, + float >_quality_score, float &reject_status_quality_score, int &qualiy_type); + //------------------------------------------------------------------ + // Functions of flow-disruption related are defined here + //! @brief Determine the flow-disruptiveness between hypotheses pairs for each read + void FlowDisruptivenessInReadLevel(const InputStructures &global_context); + //! @brief Determine the flow-disruptiveness between hypotheses pairs by looking at all reads + void FlowDisruptivenessInReadStackLevel(float min_ratio_for_fd); friend void GlueOutputVariant(EnsembleEval &my_ensemble, VariantCandidate &candidate_variant, const ExtendParameters ¶meters, int _best_allele_index, int sample_index); // I want to access your private members -// The following private members are used only in the internal steps at -// a) int DetectBestMultiAllelePair() -// b) void ApproximateHardClassifierForReads() -// c) void ApproximateHardClassifierForFamilies() private: - bool is_detect_best_multi_allele_pair_done_ = false; - // Tvc used to compute read_id etc. twice. This is not computationally efficient. - // Now we do it just once. // The following private members are the results of approximate hard classification for reads - bool is_hard_classification_for_reads_done_ = false; vector read_id_; // vector of allele ids per read, -1 = outlier, 0 = ref, >0 real allele vector strand_id_; // vector of forward (true) or reverse (false) per read // for each variant, calculate its' position within the soft clipped read distance to left and distance to right vector dist_to_left_; // vector of distances from allele position to left soft clip per read vector dist_to_right_; // vector of distances from allele position to left soft clip per read - // The following private members are the results of approximate hard classification for families - bool is_hard_classification_for_families_done_ = false; - vector family_id_; // similar to read_id, but it is for family - vector family_strand_id_; // similar to strand_id, but it is for family - vector max_family_responsibility_; // maximum family responsibility each the family in family_id + // The followings are for tag similarity + vector > alt_fam_indices_; // alt_fam_indices_[i_allele] stores the indices of families (of allele_eval.total_theory.my_eval_families) that support i_allele + vector tag_similar_counts_; }; +string PrintVariant(const vcf::Variant& variant); // just for debug message + +void FindNodesInIsoSubGraph(const vector >& connectivity_matrix, list >& subgraph_to_nodes, bool sort_by_index); +void SplitAlleleIdentityVector(const vector& allele_identity_vector, list >& allele_groups, const ReferenceReader& ref_reader, int max_group_size_allowed); +BasicFilters const * ServeBasicFilterByType(const AlleleIdentity& variant_identity, const ControlCallAndFilters& my_controls); +float FreqThresholdByType(const AlleleIdentity& variant_identity, const ControlCallAndFilters &my_controls, const VariantSpecificParams& variant_specific_params); + #endif // STACKENGINE_H diff --git a/Analysis/VariantCaller/Filter/DecisionTreeData.cpp b/Analysis/VariantCaller/Filter/DecisionTreeData.cpp index 2f5bc8f3..7b82712c 100644 --- a/Analysis/VariantCaller/Filter/DecisionTreeData.cpp +++ b/Analysis/VariantCaller/Filter/DecisionTreeData.cpp @@ -2,51 +2,29 @@ #include "DecisionTreeData.h" -void RemoveVcfInfo(vcf::Variant &variant, const vector &info_fields, const string &sample_name, int sample_index) +void RemoveVcfTagAndInfo(vcf::Variant &variant, const vector &keys, const string &sample_name, int sample_index) { - for(unsigned int i_info = 0; i_info < info_fields.size(); ++i_info){ - variant.info.erase(info_fields[i_info]); - variant.format.erase(std::remove(variant.format.begin(), variant.format.end(), info_fields[i_info]), variant.format.end()); + for(vector::const_iterator key_it = keys.begin(); key_it != keys.end(); ++key_it){ + variant.info.erase(*key_it); + variant.format.erase(std::remove(variant.format.begin(), variant.format.end(), *key_it), variant.format.end()); if (sample_index >= 0) { - variant.samples[sample_name].erase(info_fields[i_info]); + variant.samples[sample_name].erase(*key_it); } } } -void AutoFailTheCandidate(vcf::Variant &candidate_variant, bool use_position_bias, const string &sample_name, bool use_molecular_tag = false) { +void AutoFailTheCandidate(vcf::Variant &candidate_variant, bool use_position_bias, const string &sample_name, bool use_molecular_tag = false, string my_reason = "") { candidate_variant.quality = 0.0f; - NullInfoFields(candidate_variant, use_position_bias); // no information, destroy any spurious entries, add all needed tags - NullGenotypeAllSamples(candidate_variant); + NullInfoFields(candidate_variant, use_position_bias, use_molecular_tag); // no information, destroy any spurious entries, add all needed tags + NullGenotypeAllSamples(candidate_variant, use_molecular_tag); NullFilterReason(candidate_variant, sample_name); - string my_reason = "NODATA"; + if (my_reason.empty()){ + my_reason = "NODATA"; + } AddFilterReason(candidate_variant, my_reason, sample_name); SetFilteredStatus(candidate_variant, true); - - if(not use_molecular_tag){ - RemoveVcfInfo(candidate_variant, vector({"MDP", "MRO", "MAO", "MAF"}), sample_name, 0); - } } - - -float FreqThresholdByType(AlleleIdentity &variant_identity, const ControlCallAndFilters &my_controls, - const VariantSpecificParams& variant_specific_params) -{ - if (variant_specific_params.min_allele_freq_override) - return variant_specific_params.min_allele_freq; - if (variant_identity.status.isHotSpot) - return my_controls.filter_hotspot.min_allele_freq; - if (variant_identity.ActAsSNP()) - return my_controls.filter_snps.min_allele_freq; - if (variant_identity.ActAsMNP()) - return my_controls.filter_mnp.min_allele_freq; - if (variant_identity.ActAsHPIndel()) - return my_controls.filter_hp_indel.min_allele_freq; - - return my_controls.filter_snps.min_allele_freq; -} - - string EvaluatedGenotype::GenotypeAsString(){ stringstream tmp_g; tmp_g << genotype_component[0] << "/" << genotype_component[1]; @@ -54,10 +32,7 @@ string EvaluatedGenotype::GenotypeAsString(){ } bool EvaluatedGenotype::IsReference(){ - if ((genotype_component[0]==0) & (genotype_component[1]==0)) - return(true); - else - return(false); + return (genotype_component[0] == 0) and (genotype_component[1] == 0); } void PushValueOntoStringMaps(vector &tag, int index, double value) { @@ -67,30 +42,35 @@ void PushValueOntoStringMaps(vector &tag, int index, double value) { tag[index] = convertToString(d); } -void PushAlleleCountsOntoStringMaps(map >& my_map, MultiBook &all_summary_stats, bool info){ - unsigned mdp = atoi(my_map["MDP"][0].c_str()); - PushValueOntoStringMaps(my_map["FDP"], 0, all_summary_stats.TotalCount(-1)); - PushValueOntoStringMaps(my_map["FRO"], 0, all_summary_stats.GetAlleleCount(-1,0)); - PushValueOntoStringMaps(my_map["FSRF"], 0, all_summary_stats.GetAlleleCount(0,0)); - PushValueOntoStringMaps(my_map["FSRR"], 0, all_summary_stats.GetAlleleCount(1,0)); +void PushAlleleCountsOntoStringMaps(map >& my_map, MultiBook &all_summary_stats, bool use_molecular_tag){ + int fdp = all_summary_stats.TotalCount(-1); + int fro = all_summary_stats.GetAlleleCount(-1,0); + + PushValueOntoStringMaps(my_map["FDP"], 0, fdp); + PushValueOntoStringMaps(my_map["FRO"], 0, fro); + PushValueOntoStringMaps(my_map["FSRF"], 0, all_summary_stats.GetAlleleCount(0,0)); + PushValueOntoStringMaps(my_map["FSRR"], 0, all_summary_stats.GetAlleleCount(1,0)); + if (use_molecular_tag){ + PushValueOntoStringMaps(my_map["MDP"], 0, fdp); // MRO = FRO + PushValueOntoStringMaps(my_map["MRO"], 0, fro); // MRO = FRO + } // alternate allele count varies by allele - for ( int i_alt=0; i_alt< all_summary_stats.NumAltAlleles(); i_alt++){ - unsigned mao = atoi(my_map["MAO"][i_alt].c_str()); - PushValueOntoStringMaps(my_map["FAO"], i_alt, all_summary_stats.GetAlleleCount(-1,i_alt+1)); - PushValueOntoStringMaps(my_map["FSAF"], i_alt, all_summary_stats.GetAlleleCount(0,i_alt+1)); - PushValueOntoStringMaps(my_map["FSAR"], i_alt, all_summary_stats.GetAlleleCount(1,i_alt+1)); - //if (!info) { - if (all_summary_stats.TotalCount(-1) > 0) - my_map["AF"].push_back(convertToString((double)all_summary_stats.GetAlleleCount(-1,i_alt+1) / (double)all_summary_stats.TotalCount(-1))); - else - my_map["AF"].push_back(convertToString(0)); - if (mdp > 0) - my_map["MAF"].push_back(convertToString((double)mao / (double)mdp)); - else - my_map["MAF"].push_back(convertToString(0)); - //} - } + for (int i_alt=0; i_alt< all_summary_stats.NumAltAlleles(); i_alt++){ + int fao = all_summary_stats.GetAlleleCount(-1,i_alt+1); + PushValueOntoStringMaps(my_map["FAO"], i_alt, fao); + PushValueOntoStringMaps(my_map["FSAF"], i_alt, all_summary_stats.GetAlleleCount(0,i_alt+1)); + PushValueOntoStringMaps(my_map["FSAR"], i_alt, all_summary_stats.GetAlleleCount(1,i_alt+1)); + + string af_str = fdp > 0? convertToString((double) fao / (double) fdp) : "0"; + // czb: why didn't call PushValueOntoStringMaps here? + my_map["AF"].push_back(af_str); + if (use_molecular_tag){ + my_map["MAF"].push_back(af_str); // MAF = AF + PushValueOntoStringMaps(my_map["MAO"], i_alt, fao); // MAO = FAO + } + + } } float ComputeBaseStrandBiasForSSE(float relative_safety_level, vcf::Variant & candidate_variant, unsigned _altAlleleIndex, const string &sample_name){ @@ -121,14 +101,11 @@ void SuppressReferenceCalls(vcf::Variant &candidate_variant, const ExtendParamet //*** Below here is actual decision tree data ***/ - -void DecisionTreeData::SetupFromMultiAllele(const EnsembleEval &my_ensemble) { - //multi_allele = _multi_allele; - allele_identity_vector = my_ensemble.allele_identity_vector; - info_fields = my_ensemble.info_fields; -// summary_stats_vector.resize(multi_allele.allele_identity_vector.size()); - all_summary_stats.Allocate(allele_identity_vector.size()+1); // ref plus num alternate alleles - summary_info_vector.resize(allele_identity_vector.size()); +void DecisionTreeData::SetupFromMultiAllele(vector* const allele_identity_vect_ptr, vector* const info_fields_ptr){ + allele_identity_vector = allele_identity_vect_ptr; + info_fields = info_fields_ptr; + all_summary_stats.Allocate(allele_identity_vector->size() + 1); // ref plus num alternate alleles + summary_info_vector.resize(allele_identity_vector->size()); } void DecisionTreeData::OverrideFilter(vcf::Variant &candidate_variant, string & _filter_reason, int _allele, const string &sample_name) { @@ -138,26 +115,24 @@ void DecisionTreeData::OverrideFilter(vcf::Variant &candidate_variant, string & } // warning: no white-space allowed in filter reason -void FilterByBasicThresholds(const string& allele, int i_alt, MultiBook &m_summary_stats, +void DecisionTreeData::FilterByBasicThresholds(int i_alt, MultiBook &m_summary_stats, VariantOutputInfo &l_summary_info, const BasicFilters &basic_filter, float tune_xbias, float tune_sbias, - const VariantSpecificParams& variant_specific_params, - bool is_reference_call) + const VariantSpecificParams& variant_specific_params) { - int effective_min_quality_score = basic_filter.min_quality_score; - if (variant_specific_params.min_variant_score_override) - effective_min_quality_score = variant_specific_params.min_variant_score; - if (l_summary_info.variant_qual_score < effective_min_quality_score) { + // Quality Score filter + int effective_min_quality_score = variant_specific_params.min_variant_score_override ? + variant_specific_params.min_variant_score : basic_filter.min_quality_score; + if (l_summary_info.variant_qual_score < effective_min_quality_score){ string my_reason = "QualityScore<"; my_reason += convertToString(effective_min_quality_score); l_summary_info.filterReason.push_back(my_reason); l_summary_info.isFiltered = true; } - int effective_min_cov = basic_filter.min_cov; - if (variant_specific_params.min_coverage_override) - effective_min_cov = variant_specific_params.min_coverage; - + // Min Coverage filter + int effective_min_cov = variant_specific_params.min_coverage_override ? + variant_specific_params.min_coverage : basic_filter.min_cov; if (m_summary_stats.GetDepth(-1, i_alt) < effective_min_cov) { l_summary_info.isFiltered = true; string my_reason = "MINCOV<"; @@ -165,20 +140,34 @@ void FilterByBasicThresholds(const string& allele, int i_alt, MultiBook &m_summa l_summary_info.filterReason.push_back(my_reason); } - int effective_min_var_cov = basic_filter.min_var_cov; // Filter out a variant allele if the variant coverage (FAO) is too low. // Don't apply the variant coverage filter if we are making a reference call. // This is because, by applying this filter, a reference call will be filtered out if any of the variant allele fails to pass the filter, which does not make sense. - if ((not is_reference_call) and m_summary_stats.GetAlleleCount(-1, i_alt + 1) < effective_min_var_cov) { - l_summary_info.isFiltered = true; - string my_reason = "VARCOV<"; - my_reason += convertToString(effective_min_var_cov); - l_summary_info.filterReason.push_back(my_reason); + if (not eval_genotype.IsReference()){ + // Min Variant Coverage filter + int effective_min_var_cov = variant_specific_params.min_var_coverage_override ? + variant_specific_params.min_var_coverage : basic_filter.min_var_cov; + if (m_summary_stats.GetAlleleCount(-1, i_alt + 1) < effective_min_var_cov) { + l_summary_info.isFiltered = true; + string my_reason = "VARCOV<"; + my_reason += convertToString(effective_min_var_cov); + l_summary_info.filterReason.push_back(my_reason); + } + + // Min Variant Coverage filter with TGSM adjustment + if (not m_summary_stats.tag_similar_counts.empty()){ + if (m_summary_stats.GetAlleleCount(-1, i_alt + 1) - m_summary_stats.tag_similar_counts[i_alt] < effective_min_var_cov){ + l_summary_info.isFiltered = true; + string my_reason = "VARCOV-TGSM<"; + my_reason += convertToString(effective_min_var_cov); + l_summary_info.filterReason.push_back(my_reason); + } + } } - int effective_min_cov_each_strand = basic_filter.min_cov_each_strand; - if (variant_specific_params.min_coverage_each_strand_override) - effective_min_cov_each_strand = variant_specific_params.min_coverage_each_strand; + // Min Strand Coverage filter + int effective_min_cov_each_strand = variant_specific_params.min_coverage_each_strand_override ? + variant_specific_params.min_coverage_each_strand : basic_filter.min_cov_each_strand; bool pos_cov = m_summary_stats.GetDepth(0, i_alt) < effective_min_cov_each_strand; if (pos_cov){ l_summary_info.isFiltered = true; @@ -194,18 +183,18 @@ void FilterByBasicThresholds(const string& allele, int i_alt, MultiBook &m_summa l_summary_info.filterReason.push_back(my_reason); } - float effective_strand_bias_thr = basic_filter.strand_bias_threshold; - if (variant_specific_params.strand_bias_override) - effective_strand_bias_thr = variant_specific_params.strand_bias; + // STB filter + float effective_strand_bias_thr = variant_specific_params.strand_bias_override ? + variant_specific_params.strand_bias : basic_filter.strand_bias_threshold; - float effective_strand_bias_pval_thr = basic_filter.strand_bias_pval_threshold; - if (variant_specific_params.strand_bias_pval_override) - effective_strand_bias_thr = variant_specific_params.strand_bias_pval; + float effective_strand_bias_pval_thr = variant_specific_params.strand_bias_pval_override ? + variant_specific_params.strand_bias_pval : basic_filter.strand_bias_pval_threshold; float strand_bias = m_summary_stats.OldStrandBias(i_alt, tune_sbias); float strand_bias_pval = m_summary_stats.StrandBiasPval(i_alt, tune_sbias); - if (strand_bias > effective_strand_bias_thr && - strand_bias_pval <= effective_strand_bias_pval_thr) { + if ((not eval_genotype.IsReference()) + and (strand_bias > effective_strand_bias_thr) + and (strand_bias_pval <= effective_strand_bias_pval_thr)) { string my_reason = "STDBIAS"; my_reason += convertToString(strand_bias); my_reason += ">"; @@ -217,7 +206,6 @@ void FilterByBasicThresholds(const string& allele, int i_alt, MultiBook &m_summa my_reason1 += "<"; my_reason1 += convertToString(effective_strand_bias_pval_thr); l_summary_info.filterReason.push_back(my_reason1); - l_summary_info.isFiltered = true; } @@ -235,6 +223,7 @@ void FilterByBasicThresholds(const string& allele, int i_alt, MultiBook &m_summa float CalculateLod(int total_count, int min_var_cov){ float lod = 1.0f; + min_var_cov = max(min_var_cov, 1); if(total_count > 0) lod = ((float) min_var_cov - 0.5f)/((float) total_count); return lod; @@ -258,50 +247,41 @@ float CalculateOutputLod(float lod){ return output_lod; } -void DecisionTreeData::AddLodTags(vcf::Variant &candidate_variant, const string &sample_name, const ExtendParameters ¶meters){ +void DecisionTreeData::AddLodTags(vcf::Variant &candidate_variant, const string &sample_name, const ControlCallAndFilters &my_controls){ // Currently don't output LOD if use_lod_filter = false - if(not parameters.my_controls.use_lod_filter){ + if(not my_controls.use_lod_filter){ return; } int total_count = all_summary_stats.TotalCount(-1); candidate_variant.info["LOD"].clear(); - for (int i_allele = 0; i_allele < all_summary_stats.NumAltAlleles(); ++i_allele){ - int min_var_cov = 0; - //filter values specific to SNPs, MNVs and Non Homopolymer Indels - if (allele_identity_vector[i_allele].status.isHotSpot) { - min_var_cov = parameters.my_controls.filter_hotspot.min_var_cov; - } - else if (allele_identity_vector[i_allele].ActAsSNP()) { - min_var_cov = parameters.my_controls.filter_snps.min_var_cov; - } - else if (allele_identity_vector[i_allele].ActAsMNP()) { - min_var_cov = parameters.my_controls.filter_mnp.min_var_cov; - - } // end if MNV - else if (allele_identity_vector[i_allele].ActAsHPIndel()) { - min_var_cov = parameters.my_controls.filter_hp_indel.min_var_cov; - } - - float lod = CalculateLod(total_count, min_var_cov); - float output_lod = CalculateOutputLod(lod); - string val = convertToString(output_lod); - candidate_variant.info["LOD"].push_back(val); + for (unsigned int i_alt = 0; i_alt < allele_identity_vector->size(); ++i_alt){ + float output_lod = CalculateOutputLod(all_summary_stats.lod[i_alt]); + candidate_variant.info["LOD"].push_back(convertToString(output_lod)); } } -void DecisionTreeData::FilterOnLod(const string& allele, int i_alt, MultiBook &m_summary_stats, VariantOutputInfo &l_summary_info, const ControlCallAndFilters &my_filters, const BasicFilters &basic_filter, bool is_reference_call) +void DecisionTreeData::FilterOnLod(int i_alt, MultiBook &m_summary_stats, VariantOutputInfo &l_summary_info, const ControlCallAndFilters &my_filters, const BasicFilters &basic_filter) { - if((not my_filters.use_lod_filter) or is_reference_call){ + if (not my_filters.use_lod_filter){ return; } int min_var_cov = basic_filter.min_var_cov; int total_count = m_summary_stats.TotalCount(-1); - float lod = CalculateLod(total_count, min_var_cov); float min_allele_freq = basic_filter.min_allele_freq; - float af = (float) m_summary_stats.GetAlleleCount(-1, i_alt + 1) / (float)(total_count); + float lod = CalculateLod(total_count, min_var_cov); + float af = total_count > 0 ? + (float) m_summary_stats.GetAlleleCount(-1, i_alt + 1) / (float)(total_count) : 0.0f; + + if (all_summary_stats.lod.size() != allele_identity_vector->size()){ + all_summary_stats.lod.resize(allele_identity_vector->size()); + } + all_summary_stats.lod[i_alt] = lod; + + if (eval_genotype.IsReference()) + return; // The LOD filter first filter out the alt allele if af < min_allele_freq - if(af < min_allele_freq){ + if (af < min_allele_freq){ string my_reason = ""; l_summary_info.isFiltered = true; my_reason += "AF"; @@ -311,7 +291,7 @@ void DecisionTreeData::FilterOnLod(const string& allele, int i_alt, MultiBook &m l_summary_info.filterReason.push_back(my_reason); } // The LOD filter then filter out the alt allele if af < lod_multiplier * lod - if(af < my_filters.lod_multiplier * lod){ + if (af < my_filters.lod_multiplier * lod){ string my_reason = ""; l_summary_info.isFiltered = true; my_reason += "AF"; @@ -325,39 +305,35 @@ void DecisionTreeData::FilterOnLod(const string& allele, int i_alt, MultiBook &m } -void DecisionTreeData::FilterOnPositionBias(const string& allele, int i_alt, MultiBook &m_summary_stats, - VariantOutputInfo &l_summary_info, - const ControlCallAndFilters &my_filters, +void DecisionTreeData::FilterOnPositionBias(int i_alt, MultiBook &m_summary_stats, + VariantOutputInfo &l_summary_info, const ControlCallAndFilters &my_filters, const VariantSpecificParams& variant_specific_params) { - if (!my_filters.use_position_bias) { + if ((not my_filters.use_position_bias) or eval_genotype.IsReference()) { return; } - float effective_position_bias_pval_thr = my_filters.position_bias_pval; - if (variant_specific_params.position_bias_pval_override) - effective_position_bias_pval_thr = variant_specific_params.position_bias_pval; + float effective_position_bias_pval_thr = variant_specific_params.position_bias_pval_override ? + variant_specific_params.position_bias_pval : my_filters.position_bias_pval; - float effective_position_bias_thr = my_filters.position_bias; - if (variant_specific_params.position_bias_override) - effective_position_bias_thr = variant_specific_params.position_bias; + float effective_position_bias_thr = variant_specific_params.position_bias_override ? + variant_specific_params.position_bias : my_filters.position_bias; - i_alt = i_alt + 1; // confusing, ref is 0, but iterating from 0 excluding ref + int i_allele = i_alt + 1; // confusing, ref is 0, but iterating from 0 excluding ref - float position_bias = m_summary_stats.PositionBias(i_alt); - float position_bias_pval = m_summary_stats.GetPositionBiasPval(i_alt); + float position_bias = m_summary_stats.PositionBias(i_allele); + float position_bias_pval = m_summary_stats.GetPositionBiasPval(i_allele); // low fraction of ref reads is not associated with real position bias unsigned int ref_count = m_summary_stats.GetAlleleCount(-1,0); //FRO - unsigned int var_count = m_summary_stats.GetAlleleCount(-1,i_alt); //FAO - float ref_fraction = ((float)ref_count) / ((float)ref_count + (float)var_count + 0.1); + unsigned int var_count = m_summary_stats.GetAlleleCount(-1,i_allele); //FAO + float ref_fraction = ((float)ref_count) / ((float)ref_count + (float)var_count + 0.1f); - if ( ref_fraction <= my_filters.position_bias_ref_fraction ) { + if ( ref_fraction <= my_filters.position_bias_ref_fraction or my_filters.disable_filters) { return; } - if ((position_bias_pval < effective_position_bias_pval_thr) && - (position_bias > effective_position_bias_thr)){ + if ((position_bias_pval < effective_position_bias_pval_thr) and (position_bias > effective_position_bias_thr)){ string my_reason = "POSBIAS"; my_reason += convertToString(position_bias); my_reason += ">"; @@ -374,52 +350,35 @@ void DecisionTreeData::FilterOnPositionBias(const string& allele, int i_alt, Mul } } - void DecisionTreeData::FilterOneAllele(int i_alt, VariantOutputInfo &l_summary_info, AlleleIdentity &l_variant_identity, const ControlCallAndFilters &my_filters, const VariantSpecificParams& variant_specific_params) { - bool is_reference_call = eval_genotype.IsReference(); - - // if some reason from the identity to filter it - if (l_variant_identity.status.isProblematicAllele){ - l_summary_info.isFiltered = true; - for (unsigned int i_reason=0; i_reason& variant_specific_params) { for (int i_alt = 0; i_alt < all_summary_stats.NumAltAlleles(); i_alt++) { - FilterOneAllele(i_alt, summary_info_vector[i_alt], allele_identity_vector[i_alt], my_filters, variant_specific_params[i_alt]); + FilterOneAllele(i_alt, summary_info_vector[i_alt], allele_identity_vector->at(i_alt), my_filters, variant_specific_params[i_alt]); } //end loop thru alleles } @@ -427,84 +386,86 @@ void DecisionTreeData::FilterAlleles(const ControlCallAndFilters &my_filters, co ///***** heal snps here *****// void DecisionTreeData::AccumulateFilteredAlleles(){ - int numAlleles = summary_info_vector.size(); - VariantOutputInfo _summary_info; - for (int i=0; iA SNP is true then we want to move the allele representation to REF = C, Alt = A which is a more standard representation. - if (isBestAlleleSNP & heal_snps) { - //loop thru all the alleles and filter all Indel alleles which will be later removed from alts. - int numAlleles = summary_info_vector.size(); - - VariantOutputInfo _summary_info; - AlleleIdentity _variant_identity; - for (int counter = 0; counter < numAlleles; counter++) { - _summary_info = summary_info_vector[counter]; - _variant_identity = allele_identity_vector[counter]; - - - if (_variant_identity.status.isIndel && !_summary_info.isFiltered) { //if it is Indel allele and not already filtered - summary_info_vector[counter].isFiltered = true; - filteredAllelesIndex.push_back(counter); - } + if (not (isBestAlleleVeryReal and cleanup_unlikely_candidates)){ + return; + } - } - } + //loop thru all the alleles and filter all Indel alleles which will be later removed from alts. + for (unsigned int i_alt = 0; i_alt < allele_identity_vector->size(); ++i_alt){ + if (allele_identity_vector->at(i_alt).status.isHPIndel and (not summary_info_vector[i_alt].isFiltered)) { + // if it is Indel allele and not already filtered + // Note: The following step is based on the high level logic of filtering that, for X > 0 and Y > 0, X/Y PASS if either X or Y passes all filters. + // i.e., If the best allele passes, then I don't care the second best is filtered or not. + // It can be buggy if someone changes the logic. + //TODO: Make the code safe, clear and robust. + summary_info_vector[i_alt].isFiltered = true; + filteredAllelesIndex.push_back(i_alt); + } + } } -void DecisionTreeData::FindBestAlleleIdentity(){ - - AlleleIdentity _variant_identity = allele_identity_vector[best_allele_index]; - if (_variant_identity.status.isSNP || _variant_identity.status.isMNV) - isBestAlleleSNP = true; - else - isBestAlleleSNP = false; +void DecisionTreeData::FindBestAlleleIdentity(bool use_fd_param){ + if (use_fd_param){ + isBestAlleleVeryReal = allele_identity_vector->at(best_allele_index).fd_level_vs_ref == 2; + } + else{ + isBestAlleleVeryReal = allele_identity_vector->at(best_allele_index).status.isSNP or allele_identity_vector->at(best_allele_index).status.isMNV; + } } -void DecisionTreeData::SimplifySNPsIfNeeded(VariantCandidate &candidate_variant, const ExtendParameters ¶meters, const string &sample_name){ - - FindBestAlleleIdentity(); - AccumulateFilteredAlleles(); - - if (!best_variant_filtered && (isBestAlleleSNP & parameters.my_controls.heal_snps)) { //currently we are removing other filtered alleles if the best allele is a SNP +void DecisionTreeData::CleanupCandidatesIfNeeded(VariantCandidate &candidate_variant, const ExtendParameters ¶meters, const string &sample_name){ + FindBestAlleleIdentity(parameters.my_controls.use_fd_param); + AccumulateFilteredAlleles(); + // Note: CleanupCandidates only if PPA is the same as GT. + if (best_variant_filtered + or (not is_ppa_same_as_gt) + or (not isBestAlleleVeryReal) + or (not parameters.my_controls.cleanup_unlikely_candidates) + or (not eval_genotype.genotype_already_set)){ + return; + } - BestSNPsSuppressInDels(parameters.my_controls.heal_snps); + // Currently we are removing other filtered alleles if the best allele is (flow-disrupted and use_fd_param) or (SNP and not use_fd_param). + BestVariantSuppressInDels(parameters.my_controls.cleanup_unlikely_candidates); // see if any genotype-components are needed here // if so, cannot remove them // unwilling to assume "deletions" or "insertions" are really reference if they are noticeable - bool cannot_adjust = false; - if (eval_genotype.genotype_already_set){ - for (unsigned int i_ndx=0; i_ndx0){ - if (summary_info_vector[i_comp-1].isFiltered) - cannot_adjust = true; // because it is part of the best diploid genotype - } - } + for (vector::iterator gt_it = eval_genotype.genotype_component.begin(); gt_it != eval_genotype.genotype_component.end(); ++gt_it){ + if (*gt_it > 0){ + if (summary_info_vector[*gt_it - 1].isFiltered){ + return; // because it is part of the best diploid genotype + } + } } + + RemoveFilteredAlleles(candidate_variant.variant, filteredAllelesIndex, sample_name); // and therefore will give a nonsense genotype if we do adjust - if (!cannot_adjust){ - RemoveFilteredAlleles(candidate_variant.variant, filteredAllelesIndex, sample_name); - AdjustAlleles(candidate_variant.variant, candidate_variant.position_upper_bound); + // Note: The only possible GT for heal snps is 0/X and X/X where X is the best allele and it is SNP or MNP. + if (parameters.my_controls.report_ppa){ + // Update PPA to be the new GT + candidate_variant.variant.info["PPA"] = candidate_variant.variant.samples[sample_name]["GT"]; + } + if (not parameters.output_allele_cigar){ + AdjustAlleles(candidate_variant.variant, candidate_variant.position_upper_bound); } - } - } -//********* heal snps done ****//// - void DetectSSEForNoCall(VariantOutputInfo &l_summary_info, AlleleIdentity &var_identity, float sseProbThreshold, float minRatioReadsOnNonErrorStrand, float base_strand_bias,vcf::Variant & candidate_variant, unsigned _altAlleleIndex) { if (var_identity.sse_prob_positive_strand > sseProbThreshold && var_identity.sse_prob_negative_strand > sseProbThreshold) { @@ -545,7 +506,7 @@ void DecisionTreeData::FilterBlackList(const vector& vari return; // disable pending algo changes, revert to 4.2 behavior - for (unsigned int i_allele=0; i_allelesize(); i_allele++) { VariantOutputInfo &l_summary_info = summary_info_vector[i_allele]; @@ -577,25 +538,27 @@ void DecisionTreeData::FilterBlackList(const vector& vari } -void DecisionTreeData::FilterSSE(vcf::Variant &candidate_variant, const ClassifyFilters &filter_variant, const vector& variant_specific_params, const string &sample_name) +void DecisionTreeData::FilterSSE(vcf::Variant &candidate_variant, const ControlCallAndFilters &my_control, const vector& variant_specific_params, const string &sample_name) { - for (unsigned int i_allele=0; i_allelesize(); i_allele++) { // change for 4.0: store all allele information for multiallele clean filter application after VCF int _alt_allele_index = i_allele; - float base_strand_bias = ComputeBaseStrandBiasForSSE(filter_variant.sse_relative_safety_level, candidate_variant, _alt_allele_index, sample_name); + float base_strand_bias = ComputeBaseStrandBiasForSSE(my_control.filter_variant.sse_relative_safety_level, candidate_variant, _alt_allele_index, sample_name); candidate_variant.info["SSSB"].push_back(convertToString(base_strand_bias)); - candidate_variant.info["SSEP"].push_back(convertToString(allele_identity_vector[_alt_allele_index].sse_prob_positive_strand)); - candidate_variant.info["SSEN"].push_back(convertToString(allele_identity_vector[_alt_allele_index].sse_prob_negative_strand)); + candidate_variant.info["SSEP"].push_back(convertToString(allele_identity_vector->at(_alt_allele_index).sse_prob_positive_strand)); + candidate_variant.info["SSEN"].push_back(convertToString(allele_identity_vector->at(_alt_allele_index).sse_prob_negative_strand)); + if (not my_control.disable_filters){ //@TODO: make sure this takes information from the tags in candidate variant and nowhere else // which forces us to be honest and only use information in the output DetectSSEForNoCall(summary_info_vector[i_allele], - allele_identity_vector[i_allele], + allele_identity_vector->at(i_allele), variant_specific_params[_alt_allele_index].sse_prob_threshold_override ? - variant_specific_params[_alt_allele_index].sse_prob_threshold : filter_variant.sseProbThreshold, - filter_variant.minRatioReadsOnNonErrorStrand, base_strand_bias, candidate_variant, i_allele); -} + variant_specific_params[_alt_allele_index].sse_prob_threshold : my_control.filter_variant.sseProbThreshold, + my_control.filter_variant.minRatioReadsOnNonErrorStrand, base_strand_bias, candidate_variant, i_allele); + } + } } void DecisionTreeData::AddStrandBiasTags(vcf::Variant &candidate_variant, const string &sample_name){ @@ -645,22 +608,22 @@ void DecisionTreeData::AddCountInformationTags(vcf::Variant & candidate_variant, AddPositionBiasTags(candidate_variant, sample_name); // Add Dima's LOD tag - AddLodTags(candidate_variant, sample_name, parameters); + AddLodTags(candidate_variant, sample_name, parameters.my_controls); map >& infoOutput = candidate_variant.info; - PushAlleleCountsOntoStringMaps(infoOutput,all_summary_stats, true); + PushAlleleCountsOntoStringMaps(infoOutput,all_summary_stats, use_molecular_tag); infoOutput["FXX"].push_back(convertToString(all_summary_stats.GetFailedReadRatio())); if (!sample_name.empty()) { map >& sampleOutput = candidate_variant.samples[sample_name]; - PushAlleleCountsOntoStringMaps(sampleOutput, all_summary_stats, false); + PushAlleleCountsOntoStringMaps(sampleOutput, all_summary_stats, use_molecular_tag); } // hrun fill in ClearVal(candidate_variant, "HRUN"); - for (unsigned int ia=0; iasize(); ia++){ + candidate_variant.info["HRUN"].push_back(convertToString(allele_identity_vector->at(ia).ref_hp_length)); } } @@ -704,6 +667,9 @@ void DecisionTreeData::FilterOnStringency(vcf::Variant &candidate_variant, const void DecisionTreeData::FilterOnSpecialTags(vcf::Variant & candidate_variant, const ExtendParameters ¶meters, const vector& variant_specific_params, const string &sample_name) { + if (parameters.my_controls.disable_filters){ + return; + } // separate my control here /* float max_bias = 0.0f; @@ -712,7 +678,7 @@ void DecisionTreeData::FilterOnSpecialTags(vcf::Variant & candidate_variant, con if (abs(bias) > max_bias) max_bias = abs(bias); } */ - for (unsigned int _alt_allele_index = 0; _alt_allele_index < allele_identity_vector.size(); _alt_allele_index++) { + for (unsigned int _alt_allele_index = 0; _alt_allele_index < allele_identity_vector->size(); _alt_allele_index++) { // if something is strange here /* Not to do this on allele, revert to 4.6*/ SpecializedFilterFromLatentVariables(*(variant), variant_specific_params[_alt_allele_index].filter_unusual_predictions_override ? @@ -730,12 +696,12 @@ void DecisionTreeData::FilterOnSpecialTags(vcf::Variant & candidate_variant, con } */ - SpecializedFilterFromHypothesisBias(*(variant), allele_identity_vector[_alt_allele_index], + SpecializedFilterFromHypothesisBias(*(variant), allele_identity_vector->at(_alt_allele_index), variant_specific_params[_alt_allele_index].filter_deletion_predictions_override ? variant_specific_params[_alt_allele_index].filter_deletion_predictions : parameters.my_eval_control.filter_deletion_bias, variant_specific_params[_alt_allele_index].filter_insertion_predictions_override ? variant_specific_params[_alt_allele_index].filter_insertion_predictions : parameters.my_eval_control.filter_insertion_bias, - _alt_allele_index, sample_name); + _alt_allele_index, sample_name, parameters.my_controls.use_fd_param); float effective_data_quality_stringency = parameters.my_controls.data_quality_stringency; if (variant_specific_params[_alt_allele_index].data_quality_stringency_override) @@ -791,23 +757,35 @@ void DecisionTreeData::FilterAlleleHypothesisBias(vcf::Variant & candidate_varia } - -void DecisionTreeData::SpecializedFilterFromHypothesisBias(vcf::Variant & candidate_variant, AlleleIdentity allele_identity, const float deletion_bias, const float insertion_bias, int _allele, const string &sample_name) +void DecisionTreeData::SpecializedFilterFromHypothesisBias(vcf::Variant & candidate_variant, AlleleIdentity allele_identity, const float deletion_bias, const float insertion_bias, int _allele, const string &sample_name, bool use_fd_param) { - -// float ref_bias = hypothesis_stack.cur_state.bias_generator.latent_bias_v[0]; -// float var_bias = hypothesis_stack.cur_state.bias_generator.latent_bias_v[1]; - float ref_bias = RetrieveQualityTagValue(candidate_variant, "REFB", _allele); - float var_bias = RetrieveQualityTagValue(candidate_variant, "VARB", _allele); - - if (allele_identity.ActAsHPIndel()) { - if (allele_identity.status.isDeletion) { - FilterAlleleHypothesisBias(candidate_variant, ref_bias, var_bias, deletion_bias, _allele, sample_name); + float ref_bias = RetrieveQualityTagValue(candidate_variant, "REFB", _allele); + float var_bias = RetrieveQualityTagValue(candidate_variant, "VARB", _allele); + float bias_threshold = -1.0f; + if (use_fd_param){ + if (allele_identity.fd_level_vs_ref < 2){ + // Now the ref bias and var bias filters can be applied to non-fd snp. + // Non-fd snp is a combo of INS + DEL, so I will use the minimum bias threshold. + if (allele_identity.status.isDeletion){ + bias_threshold = deletion_bias; + }else if (allele_identity.status.isInsertion){ + bias_threshold = insertion_bias; + }else{ + bias_threshold = min(deletion_bias, insertion_bias); + } + } + }else if (allele_identity.ActAsHPIndel()){ + // The old way that we do bias filtering. + if (allele_identity.status.isDeletion){ + bias_threshold = deletion_bias; + }else if (allele_identity.status.isInsertion){ + bias_threshold = insertion_bias; + } } - else if (allele_identity.status.isInsertion) { - FilterAlleleHypothesisBias(candidate_variant, ref_bias, var_bias, insertion_bias, _allele, sample_name); + + if (bias_threshold > 0.0f){ + FilterAlleleHypothesisBias(candidate_variant, ref_bias, var_bias, bias_threshold, _allele, sample_name); } - } } void FilterOnReadRejectionRate(vcf::Variant &candidate_variant, float read_rejection_threshold, const string &sample_name){ @@ -828,14 +806,13 @@ void DecisionTreeData::AggregateFilterInformation(vcf::Variant & candidate_varia // adds tags to the file FilterBlackList(variant_specific_params); - FilterSSE(candidate_variant, parameters.my_controls.filter_variant, variant_specific_params, sample_name); + FilterSSE(candidate_variant, parameters.my_controls, variant_specific_params, sample_name); FilterOnSpecialTags(candidate_variant, parameters, variant_specific_params, sample_name); FilterAlleles(parameters.my_controls, variant_specific_params); AddCountInformationTags(candidate_variant, sample_name, parameters); - } @@ -880,8 +857,8 @@ void DecisionTreeData::GenotypeAlleleFilterMyCandidate(vcf::Variant &candidate_ } // Abuse FR tag to write out info fields - for (unsigned int i_info=0; i_infosize(); i_info++) + AddInfoReason(candidate_variant, info_fields->at(i_info), sample_name); // if no-one escaped if (!no_filter){ @@ -909,10 +886,61 @@ void DecisionTreeData::FilterMyCandidate(vcf::Variant &candidate_variant, const GenotypeAlleleFilterMyCandidate(candidate_variant, parameters, sample_name); // whole candidate reason - FilterOnReadRejectionRate(candidate_variant, parameters.my_controls.read_rejection_threshold, sample_name); + if (not parameters.my_controls.disable_filters){ + FilterOnReadRejectionRate(candidate_variant, parameters.my_controls.read_rejection_threshold, sample_name); + } } +// Determine Possible Polyploidy Alleles (PPA) +// An allele is a PPA if the conditions a) and b) are both satisfied. +// a) Called in GT or its estimated allele frequency > min-allele-freq +// b) Pass all the filters (e.g., STB, RBI, QUAL, etc.) +// (Note): Must be done before heal SNPs +// (Note): Report "." if none +void DecisionTreeData::DeterminePossiblePolyploidyAlleles(vcf::Variant &candidate_variant, const ExtendParameters ¶meters) { + unsigned int num_alleles = allele_identity_vector->size() + 1; + if (is_possible_polyploidy_allele.size() != num_alleles){ + is_possible_polyploidy_allele.assign(num_alleles, false); + } + // The alleles in GT are PPA + is_possible_polyploidy_allele[eval_genotype.genotype_component[0]] = true; + is_possible_polyploidy_allele[eval_genotype.genotype_component[1]] = true; + + if (not parameters.my_controls.report_ppa){ + is_ppa_same_as_gt = true; + return; + } + + + string ppa_string = ""; + ppa_string.reserve(2 * num_alleles); + int num_ppa_alleles = 0; // number of alleles in PPA + for (unsigned int i_allele = 0; i_allele < num_alleles; ++i_allele){ + if (i_allele > 0){ + is_possible_polyploidy_allele[i_allele] = is_possible_polyploidy_allele[i_allele] and (not summary_info_vector[i_allele - 1].isFiltered); + } + if (is_possible_polyploidy_allele[i_allele]){ + ppa_string += (to_string(i_allele) + "/"); + ++num_ppa_alleles; + } + } + int num_gt_alleles = (eval_genotype.genotype_component[0] == eval_genotype.genotype_component[1])? 1:2; + + is_ppa_same_as_gt = is_possible_polyploidy_allele[eval_genotype.genotype_component[0]] // Does the first allele of GT get filtered? + and is_possible_polyploidy_allele[eval_genotype.genotype_component[1]] // Does the second allele in GT get filtered? + and (num_ppa_alleles == num_gt_alleles); // Does GT and PPA have the same number of alleles? + + if (is_ppa_same_as_gt){ + ppa_string = eval_genotype.GenotypeAsString(); + }else if (ppa_string.empty()){ + ppa_string = "."; + }else{ + ppa_string.resize(ppa_string.size() - 1); // remove the last "/" + } + candidate_variant.info["PPA"] = {ppa_string}; +} + void DecisionTreeData::FillInFiltersAtEnd(VariantCandidate &candidate_variant, const ExtendParameters ¶meters, const string &sample_name) { @@ -924,161 +952,15 @@ void DecisionTreeData::FillInFiltersAtEnd(VariantCandidate &candidate_variant, c // candidate alleles contribute to possible filtration FilterMyCandidate(candidate_variant.variant, parameters, sample_name); - SimplifySNPsIfNeeded(candidate_variant, parameters, sample_name); + CleanupCandidatesIfNeeded(candidate_variant, parameters, sample_name); // if the genotype is a reference call, and we want to suppress it, make sure it will be in the filtered file SuppressReferenceCalls(candidate_variant.variant, parameters, reference_genotype, sample_name); if (parameters.my_controls.suppress_nocall_genotypes) DetectAndSetFilteredGenotype(candidate_variant.variant, variant_quality, sample_name); -} - - -// once we have standard data format across all alleles, the common filters can execute. -// this is just horrible at the moment -// need to clean this code up badly/* Copyright (C) 2013 Ion Torrent Systems, Inc. All Rights Reserved */ - - -/* Copyright (C) 2013 Ion Torrent Systems, Inc. All Rights Reserved */ -#ifndef DECISIONTREEDATA_H -#define DECISIONTREEDATA_H - - -#include "api/BamReader.h" - -#include -#include -#include -#include -#include -#include -#include - -#include "ClassifyVariant.h" -#include "StackEngine.h" -#include "VcfFormat.h" - -class EvaluatedGenotype{ -public: - bool genotype_already_set; - float evaluated_genotype_quality; - float evaluated_variant_quality; - vector genotype_component; - - EvaluatedGenotype(){ - genotype_already_set = false; - evaluated_genotype_quality = 0.0f; - evaluated_variant_quality = 0.0f; - genotype_component.assign(2,0); // 0/0 = reference call - - }; - string GenotypeAsString(); - bool IsReference(); -}; - -// all the data needed to make a decision for filtration -// characterize the variant, and the outcome from whatever evaluator we use -class DecisionTreeData { - public: - - vcf::Variant * variant; //!< VCF record of this variant position - vector allele_identity_vector; //!< Detailed information for each candidate allele - vector info_fields; //!< Additional information to be printed out in vcf FR tag - - MultiBook all_summary_stats; - - vector summary_info_vector; - - vector filteredAllelesIndex; - - map variant_quality; - - - bool best_variant_filtered; - - bool best_allele_set; - int best_allele_index; - bool isBestAlleleSNP; - bool reference_genotype; - - EvaluatedGenotype eval_genotype; - - float tune_xbias; // not tuned, removed from filters - float tune_sbias; - - DecisionTreeData(vcf::Variant &candidate_variant) /*: multi_allele(candidate_variant)*/ { - variant = &candidate_variant; - best_allele_set = false; - best_allele_index = 0; - best_variant_filtered=false; - isBestAlleleSNP = false; - reference_genotype = false; - - - tune_xbias = 0.005f; // tune calculation of chi-square bias = proportioinal variance by frequency - tune_sbias = 0.5f; // safety factor for small allele counts for transformed strand bias - }; - - void OverrideFilter(vcf::Variant &candidate_variant, string & _filter_reason, int _allele, const string &sample_name); - void FilterOneAllele(int i_alt,VariantOutputInfo &l_summary_info, - AlleleIdentity &l_variant_identity, const ControlCallAndFilters &my_filters, - const VariantSpecificParams& variant_specific_params); - void FilterAlleles(const ControlCallAndFilters &my_filters, const vector& variant_specific_params); - - void AccumulateFilteredAlleles(); - - void BestSNPsSuppressInDels(bool heal_snps); - void FindBestAlleleIdentity(); - void FindBestAlleleByScore(); - - void GenotypeFromBestAlleleIndex(vcf::Variant &candidate_variant, const ExtendParameters ¶meters); - void GenotypeFromEvaluator(vcf::Variant &candidate_variant, const ExtendParameters ¶meters, const string &sample_name); - - void FilterMyCandidate(vcf::Variant &candidate_variant, const ExtendParameters ¶meters, const string &sample_name); - void BestAlleleFilterMyCandidate(vcf::Variant &candidate_variant, const ExtendParameters ¶meters); - void GenotypeAlleleFilterMyCandidate(vcf::Variant &candidate_variant, const ExtendParameters ¶meters, const string &sample_name); - - void SimplifySNPsIfNeeded(VariantCandidate &candidate_variant, const ExtendParameters ¶meters, const string &sample_name); - - - bool SetGenotype(vcf::Variant &candidate_variant, const ExtendParameters ¶meters, float gt_quality); - void DecisionTreeOutputToVariant(VariantCandidate &candidate_variant, const ExtendParameters ¶meters, int sample_index); - - void AggregateFilterInformation(vcf::Variant &candidate_variant, const vector& variant_specific_params, const ExtendParameters ¶meters, const string &sample_name); - void FillInFiltersAtEnd(VariantCandidate &candidate_variant,const ExtendParameters ¶meters, const string &sample_name); - - - - void SetupFromMultiAllele(const EnsembleEval &my_ensemble); - void AddStrandBiasTags(vcf::Variant &candidate_variant, const string &sample_name); - void AddPositionBiasTags(vcf::Variant &candidate_variant, const string &sample_name); - void AddLodTags(vcf::Variant &candidate_variant, const string &sample_name, const ExtendParameters ¶meters); - void AddCountInformationTags(vcf::Variant &candidate_variant, const string &sampleName, const ExtendParameters ¶meters); - string GenotypeStringFromAlleles(std::vector &allowedGenotypes, bool refAlleleFound); - bool AllowedGenotypesFromSummary(std::vector &allowedGenotypes); - string GenotypeFromStatus(vcf::Variant &candidate_variant, const ExtendParameters ¶meters); - void SpecializedFilterFromLatentVariables(vcf::Variant &candidate_variant, const float bias_radius, int _allele, const string &sample_name); - void SpecializedFilterFromHypothesisBias(vcf::Variant &candidate_variant, AlleleIdentity allele_identity, const float deletion_bias, const float insertion_bias, int _allele, const string &sample_name); - void FilterAlleleHypothesisBias(vcf::Variant &candidate_variant, float ref_bias, float var_bias, float threshold_bias, int _allele, const string &sample_name); - void FilterOnSpecialTags(vcf::Variant &candidate_variant, const ExtendParameters ¶meters, const vector& variant_specific_params, const string &sample_name); - void FilterOnStringency(vcf::Variant &candidate_variant, const float data_quality_stringency, int _check_allele_index, const string &sample_name); - void FilterOnPositionBias(const string& allele, int i_alt, MultiBook &m_summary_stats, VariantOutputInfo &l_summary_info, const ControlCallAndFilters &my_filters, const VariantSpecificParams& variant_specific_params); - void FilterOnLod(const string& allele, int i_alt, MultiBook &m_summary_stats, VariantOutputInfo &l_summary_info, const ControlCallAndFilters &my_filters, const BasicFilters &basic_filter, bool is_reference_call); - void FilterBlackList(const vector& variant_specific_params); - void FilterSSE(vcf::Variant &candidate_variant, const ClassifyFilters &filter_variant, const vector& variant_specific_params, const string &sample_name); -}; -void FilterByBasicThresholds(stringstream &s, int i_alt, MultiBook &m_summary_stats, - VariantOutputInfo &l_summary_info, - const BasicFilters &basic_filter, float tune_xbias, float tune_bias, bool is_reference_call); - -void AutoFailTheCandidate(vcf::Variant &candidate_variant, bool use_position_bias, const string &sample_name); -float FreqThresholdByType(AlleleIdentity &variant_identity, const ControlCallAndFilters &my_controls, const VariantSpecificParams& variant_specific_params); -void DetectSSEForNoCall(AlleleIdentity &var_identity, float sseProbThreshold, float minRatioReadsOnNonErrorStrand, float relative_safety_level, vcf::Variant &candidate_variant, unsigned _altAlleIndex); -void SetQualityByDepth(vcf::Variant &candidate_variant, map& variant_quality, const string &sample_name); - -#endif // DECISIONTREEDATA_H - +} /* 0) A variant entry accumulates all relevant data before being filtered: @@ -1105,7 +987,7 @@ void SetQualityByDepth(vcf::Variant &candidate_variant, map& vari 3y) the QUAL score is below the min-variant-score 3a) if coverage (ref+allele) is too low in total (low quality of data) 3b) if coverage (ref+allele) is insufficient on either strand (low quality of data) -3c) if strand bias is triggered for this allele vs reference (model violation) +3c) if not a ref call and strand bias is triggered for this allele vs reference (model violation) 3cc) if the strand-beta-bias is triggered for this allele vs reference (model violation) 3d) The allele fails STRINGENCY against reference (general signal/noise) 3e) The allele has a PREDICTIONSHIFT (systematic mismatch between predictions and measurements in basecalling) @@ -1115,14 +997,13 @@ void SetQualityByDepth(vcf::Variant &candidate_variant, map& vari */ void DecisionTreeData::DecisionTreeOutputToVariant(VariantCandidate &candidate_variant, const ExtendParameters ¶meters, int sample_index) { - string sample_name = ""; - if (sample_index >= 0) {sample_name = candidate_variant.variant.sampleNames[sample_index];} + string sample_name = sample_index >= 0? candidate_variant.variant.sampleNames[sample_index] : ""; AggregateFilterInformation(candidate_variant.variant, candidate_variant.variant_specific_params, parameters, sample_name); // step 0 above - GenotypeFromEvaluator(candidate_variant.variant, parameters, sample_name); // step 0 // add a derived tag from the QUAL field and the depth counts SetQualityByDepth(candidate_variant.variant, variant_quality, sample_name); - + DeterminePossiblePolyploidyAlleles(candidate_variant.variant, parameters); // no actual filters should be filled in yet, just all the information needed for filtering FillInFiltersAtEnd(candidate_variant, parameters, sample_name); + } diff --git a/Analysis/VariantCaller/Filter/DecisionTreeData.h b/Analysis/VariantCaller/Filter/DecisionTreeData.h index 03843e9d..8b30b4e7 100644 --- a/Analysis/VariantCaller/Filter/DecisionTreeData.h +++ b/Analysis/VariantCaller/Filter/DecisionTreeData.h @@ -43,15 +43,15 @@ class DecisionTreeData { public: vcf::Variant * variant; //!< VCF record of this variant position - vector allele_identity_vector; //!< Detailed information for each candidate allele - vector info_fields; //!< Additional information to be printed out in vcf FR tag + vector* allele_identity_vector; //!< Detailed information for each candidate allele + vector* info_fields; //!< Additional information to be printed out in vcf FR tag MultiBook all_summary_stats; vector summary_info_vector; vector filteredAllelesIndex; - + vector is_possible_polyploidy_allele; map variant_quality; @@ -59,9 +59,10 @@ class DecisionTreeData { bool best_allele_set; int best_allele_index; - bool isBestAlleleSNP; + bool isBestAlleleVeryReal; bool reference_genotype; - + bool is_ppa_same_as_gt; + bool use_molecular_tag; EvaluatedGenotype eval_genotype; float tune_xbias; // not tuned, removed from filters @@ -69,12 +70,15 @@ class DecisionTreeData { DecisionTreeData(vcf::Variant &candidate_variant) /*: multi_allele(candidate_variant)*/ { variant = &candidate_variant; + allele_identity_vector = NULL; + info_fields = NULL; best_allele_set = false; best_allele_index = 0; best_variant_filtered=false; - isBestAlleleSNP = false; + isBestAlleleVeryReal = false; reference_genotype = false; - + is_ppa_same_as_gt = true; + use_molecular_tag = false; tune_xbias = 0.005f; // tune calculation of chi-square bias = proportioinal variance by frequency tune_sbias = 0.5f; // safety factor for small allele counts for transformed strand bias @@ -88,8 +92,10 @@ class DecisionTreeData { void AccumulateFilteredAlleles(); - void BestSNPsSuppressInDels(bool heal_snps); + void BestVariantSuppressInDels(bool heal_snps); void FindBestAlleleIdentity(); + void FindBestAlleleIdentity(bool use_fd_param); + void FindBestAlleleByScore(); void GenotypeFromBestAlleleIndex(vcf::Variant &candidate_variant, const ExtendParameters ¶meters); @@ -99,8 +105,7 @@ class DecisionTreeData { void BestAlleleFilterMyCandidate(vcf::Variant &candidate_variant, const ExtendParameters ¶meters); void GenotypeAlleleFilterMyCandidate(vcf::Variant &candidate_variant, const ExtendParameters ¶meters, const string &sample_name); - void SimplifySNPsIfNeeded(VariantCandidate &candidate_variant, const ExtendParameters ¶meters, const string &sample_name); - + void CleanupCandidatesIfNeeded(VariantCandidate &candidate_variant, const ExtendParameters ¶meters, const string &sample_name); bool SetGenotype(vcf::Variant &candidate_variant, const ExtendParameters ¶meters, float gt_quality); void DecisionTreeOutputToVariant(VariantCandidate &candidate_variant, const ExtendParameters ¶meters, int sample_index); @@ -108,34 +113,35 @@ class DecisionTreeData { void AggregateFilterInformation(vcf::Variant &candidate_variant, const vector& variant_specific_params, const ExtendParameters ¶meters, const string &sample_name); void FillInFiltersAtEnd(VariantCandidate &candidate_variant,const ExtendParameters ¶meters, const string &sample_name); + void SetupFromMultiAllele(vector* const allele_identity_vect_ptr, vector* const info_fields_ptr); - - void SetupFromMultiAllele(const EnsembleEval &my_ensemble); void AddStrandBiasTags(vcf::Variant &candidate_variant, const string &sample_name); void AddPositionBiasTags(vcf::Variant &candidate_variant, const string &sample_name); - void AddLodTags(vcf::Variant &candidate_variant, const string &sample_name, const ExtendParameters ¶meters); + void AddLodTags(vcf::Variant &candidate_variant, const string &sample_name, const ControlCallAndFilters &my_control); void AddCountInformationTags(vcf::Variant &candidate_variant, const string &sampleName, const ExtendParameters ¶meters); string GenotypeStringFromAlleles(std::vector &allowedGenotypes, bool refAlleleFound); bool AllowedGenotypesFromSummary(std::vector &allowedGenotypes); string GenotypeFromStatus(vcf::Variant &candidate_variant, const ExtendParameters ¶meters); void SpecializedFilterFromLatentVariables(vcf::Variant &candidate_variant, const float bias_radius, int _allele, const string &sample_name); - void SpecializedFilterFromHypothesisBias(vcf::Variant &candidate_variant, AlleleIdentity allele_identity, const float deletion_bias, const float insertion_bias, int _allele, const string &sample_name); + void SpecializedFilterFromHypothesisBias(vcf::Variant &candidate_variant, AlleleIdentity allele_identity, const float deletion_bias, const float insertion_bias, int _allele, const string &sample_name, bool use_fd_param); + void FilterByBasicThresholds(int i_alt, MultiBook &m_summary_stats, VariantOutputInfo &l_summary_info, + const BasicFilters &basic_filter, float tune_xbias, float tune_bias, const VariantSpecificParams& variant_specific_params); + void FilterAlleleHypothesisBias(vcf::Variant &candidate_variant, float ref_bias, float var_bias, float threshold_bias, int _allele, const string &sample_name); void FilterOnSpecialTags(vcf::Variant &candidate_variant, const ExtendParameters ¶meters, const vector& variant_specific_params, const string &sample_name); void FilterOnStringency(vcf::Variant &candidate_variant, const float data_quality_stringency, int _check_allele_index, const string &sample_name); - void FilterOnPositionBias(const string& allele, int i_alt, MultiBook &m_summary_stats, VariantOutputInfo &l_summary_info, const ControlCallAndFilters &my_filters, const VariantSpecificParams& variant_specific_params); - void FilterOnLod(const string& allele, int i_alt, MultiBook &m_summary_stats, VariantOutputInfo &l_summary_info, const ControlCallAndFilters &my_filters, const BasicFilters &basic_filter, bool is_reference_call); + void FilterOnPositionBias(int i_alt, MultiBook &m_summary_stats, VariantOutputInfo &l_summary_info, const ControlCallAndFilters &my_filters, const VariantSpecificParams& variant_specific_params); + void FilterOnLod(int i_alt, MultiBook &m_summary_stats, VariantOutputInfo &l_summary_info, const ControlCallAndFilters &my_filters, const BasicFilters &basic_filter); + void FilterBlackList(const vector& variant_specific_params); - void FilterSSE(vcf::Variant &candidate_variant, const ClassifyFilters &filter_variant, const vector& variant_specific_params, const string &sample_name); + void FilterSSE(vcf::Variant &candidate_variant, const ControlCallAndFilters &my_control, const vector& variant_specific_params, const string &sample_name); + void DeterminePossiblePolyploidyAlleles(vcf::Variant &candidate_variant, const ExtendParameters ¶meters); }; -void FilterByBasicThresholds(stringstream &s, int i_alt, MultiBook &m_summary_stats, - VariantOutputInfo &l_summary_info, - const BasicFilters &basic_filter, float tune_xbias, float tune_bias, bool is_reference_call); -void AutoFailTheCandidate(vcf::Variant &candidate_variant, bool use_position_bias, const string &sample_name, bool use_molecular_tag); -float FreqThresholdByType(AlleleIdentity &variant_identity, const ControlCallAndFilters &my_controls, const VariantSpecificParams& variant_specific_params); + +void AutoFailTheCandidate(vcf::Variant &candidate_variant, bool use_position_bias, const string &sample_name, bool use_molecular_tag, string my_reason); void DetectSSEForNoCall(AlleleIdentity &var_identity, float sseProbThreshold, float minRatioReadsOnNonErrorStrand, float relative_safety_level, vcf::Variant &candidate_variant, unsigned _altAlleIndex); void SetQualityByDepth(vcf::Variant &candidate_variant, map& variant_quality, const string &sample_name); -void RemoveVcfInfo(vcf::Variant &variant, const vector &info_fields, const string &sample_name, int sample_index); +void RemoveVcfTagAndInfo(vcf::Variant &variant, const vector &info_fields, const string &sample_name, int sample_index); #endif // DECISIONTREEDATA_H diff --git a/Analysis/VariantCaller/Filter/VariantAssist.h b/Analysis/VariantCaller/Filter/VariantAssist.h index 501d7b6b..5bc961e2 100644 --- a/Analysis/VariantCaller/Filter/VariantAssist.h +++ b/Analysis/VariantCaller/Filter/VariantAssist.h @@ -49,6 +49,8 @@ class MultiBook { // alleles = 0 (ref), 1,2,... alts vector< vector > my_book; int invalid_reads; + vector tag_similar_counts; // counts for similar molecular tags + vector lod; // limit of detection for tag seq MultiBook(); void Allocate(int num_hyp); diff --git a/Analysis/VariantCaller/HandleVariant.cpp b/Analysis/VariantCaller/HandleVariant.cpp index 6cb48a4e..4ddb38ea 100644 --- a/Analysis/VariantCaller/HandleVariant.cpp +++ b/Analysis/VariantCaller/HandleVariant.cpp @@ -6,79 +6,13 @@ #include "HandleVariant.h" #include "DecisionTreeData.h" - - -void EnsembleEval::SpliceAllelesIntoReads(PersistingThreadObjects &thread_objects, const InputStructures &global_context, - const ExtendParameters ¶meters, const ReferenceReader &ref_reader, int chr_idx) -{ - bool changed_alignment; - unsigned int num_valid_reads = 0; - unsigned int num_realigned = 0; - int num_hyp_no_null = allele_identity_vector.size()+1; // num alleles +1 for ref - - // generate null+ref+nr.alt hypotheses per read in the case of do_multiallele_eval - allele_eval.total_theory.my_hypotheses.resize(read_stack.size()); - - for (unsigned int i_read = 0; i_read < allele_eval.total_theory.my_hypotheses.size(); i_read++) { - // --- New splicing function --- - allele_eval.total_theory.my_hypotheses[i_read].success = - SpliceVariantHypotheses(*read_stack[i_read], - *this, - seq_context, - thread_objects, - allele_eval.total_theory.my_hypotheses[i_read].splice_start_flow, - allele_eval.total_theory.my_hypotheses[i_read].splice_end_flow, - allele_eval.total_theory.my_hypotheses[i_read].instance_of_read_by_state, - allele_eval.total_theory.my_hypotheses[i_read].same_as_null_hypothesis, - changed_alignment, - global_context, - ref_reader, chr_idx); - - if (allele_eval.total_theory.my_hypotheses[i_read].success){ - num_valid_reads++; - if (changed_alignment) - num_realigned++; - } - - // if we need to compare likelihoods across multiple possibilities - if (num_hyp_no_null > 2) - allele_eval.total_theory.my_hypotheses[i_read].use_correlated_likelihood = false; - } - - // Check how many reads had their alignment modified - std::ostringstream my_info; - my_info.precision(4); - if (doRealignment and num_valid_reads>0){ - float frac_realigned = (float)num_realigned / (float)num_valid_reads; - // And re-do splicing without realignment if we exceed the threshold - if (frac_realigned > parameters.my_controls.filter_variant.realignment_threshold){ - my_info << "SKIPREALIGNx" << frac_realigned; - doRealignment = false; - for (unsigned int i_read = 0; i_read < allele_eval.total_theory.my_hypotheses.size(); i_read++) { - allele_eval.total_theory.my_hypotheses[i_read].success = - SpliceVariantHypotheses(*read_stack[i_read], - *this, - seq_context, - thread_objects, - allele_eval.total_theory.my_hypotheses[i_read].splice_start_flow, - allele_eval.total_theory.my_hypotheses[i_read].splice_end_flow, - allele_eval.total_theory.my_hypotheses[i_read].instance_of_read_by_state, - allele_eval.total_theory.my_hypotheses[i_read].same_as_null_hypothesis, - changed_alignment, - global_context, - ref_reader, chr_idx); - } - } - else { - my_info << "REALIGNEDx" << frac_realigned; - } - info_fields.push_back(my_info.str()); - } - -} - - - +#include "StackEngine.h" +#include "ShortStack.h" +#include "MiscUtil.h" +#include "ExtendedReadInfo.h" +#include "ClassifyVariant.h" +#include "DiagnosticJson.h" +#include "ExtendParameters.h" void SummarizeInfoFieldsFromEnsemble(EnsembleEval &my_ensemble, vcf::Variant &candidate_variant, int _cur_allele_index, const string &sample_name) { @@ -112,601 +46,370 @@ void SummarizeInfoFieldsFromEnsemble(EnsembleEval &my_ensemble, vcf::Variant &ca candidate_variant.info["VARB"].push_back(convertToString(var_bias)); } -// The structure InferenceResult is used for void MultiMinAlleleFreq(...) only. -// qual, gt, gq are the QUAL, GT, GQ computed for min_allele_freq, respectively -struct InferenceResult{ - float min_allele_freq; - float qual; - string gt; - int gq; - bool operator<(const InferenceResult &rhs) const { return min_allele_freq < rhs.min_allele_freq; } // use the operator "<" for "std::sort" - bool operator==(const float &rhs) const { return min_allele_freq == rhs; } // use the operator "==" for "std::find" -}; - -void MultiMinAlleleFreq(EnsembleEval &my_ensemble, VariantCandidate &candidate_variant, int sample_index, ProgramControlSettings &program_flow, int max_detail_level){ - string sample_name = ""; - if(sample_index >= 0) { - sample_name = candidate_variant.variant.sampleNames[sample_index]; - } - - // info tags needed for multi-min-allele-freq - vector tags_for_multi_min_allele_freq = {"MUAF", "MUQUAL", "MUGQ", "MUGT", - "SMAF", "SMQUAL", "SMGQ", "SMGT", - "MMAF", "MMQUAL", "MMGQ", "MMGT", - "IMAF", "IMQUAL", "IMGQ", "IMGT", - "HMAF", "HMQUAL", "HMGQ", "HMGT"}; - // Add the info tags for multi-min-allele-freq if we have not added yet. - for(unsigned int i_tag = 0; i_tag < tags_for_multi_min_allele_freq.size(); ++i_tag){ - vector::iterator it_format = find(candidate_variant.variant.format.begin(), - candidate_variant.variant.format.end(), - tags_for_multi_min_allele_freq[i_tag]); - if(it_format == candidate_variant.variant.format.end()){ - candidate_variant.variant.format.push_back(tags_for_multi_min_allele_freq[i_tag]); - } - } - - // inference_results_union stores the inference results for the union of the min-allele-freq of all available variant types - vector inference_results_union; - bool is_snp_done = false; - bool is_mnp_done = false; - bool is_hotspot_done = false; - bool is_indel_done = false; - - for(unsigned int alt_allele_index = 0; alt_allele_index < my_ensemble.allele_identity_vector.size(); ++alt_allele_index){ - // ptr_maf_vec = the pointer to the multi_min_allele_freq vector of which type of variant for this allele - vector *ptr_maf_vec = &(program_flow.snp_multi_min_allele_freq); - string type_prefix = "S"; - - // Note that no override here! - if(my_ensemble.allele_identity_vector[alt_allele_index].status.isHotSpot){ - if(is_hotspot_done){ - continue; - } - ptr_maf_vec = &(program_flow.hotspot_multi_min_allele_freq); - type_prefix = "H"; - } - else if(my_ensemble.allele_identity_vector[alt_allele_index].ActAsSNP()){ - if(is_snp_done){ - continue; - } - ptr_maf_vec = &(program_flow.snp_multi_min_allele_freq); - type_prefix = "S"; - } - else if(my_ensemble.allele_identity_vector[alt_allele_index].ActAsMNP()){ - if(is_mnp_done){ - continue; - } - ptr_maf_vec = &(program_flow.mnp_multi_min_allele_freq); - type_prefix = "M"; - } - else if(my_ensemble.allele_identity_vector[alt_allele_index].ActAsHPIndel()){ - if(is_indel_done){ - continue; - } - ptr_maf_vec = &(program_flow.indel_multi_min_allele_freq); - type_prefix = "I"; - }else{ - if(is_snp_done){ - continue; - } - ptr_maf_vec = &(program_flow.snp_multi_min_allele_freq); - string type_prefix = "S"; - } - - for(unsigned int i_freq = 0; i_freq < ptr_maf_vec->size(); ++i_freq){ - float loc_min_allele_freq = ptr_maf_vec->at(i_freq); - vector::iterator it = find(inference_results_union.begin(), inference_results_union.end(), loc_min_allele_freq); - float loc_qual; - int loc_gq; - string loc_gt; - - if(it == inference_results_union.end()){ // This the first time we get loc_min_allele_freq - int genotype_call; - float evaluated_genotype_quality; - // Let's do the inference for the given loc_min_allele_freq - my_ensemble.allele_eval.CallGermline(loc_min_allele_freq, genotype_call, evaluated_genotype_quality, loc_qual); - - vector genotype_component = {my_ensemble.diploid_choice[0], my_ensemble.diploid_choice[1]}; // starts with het var - - if(genotype_call == 2){ //hom var - genotype_component[0] = my_ensemble.diploid_choice[1]; - } - else if(genotype_call == 0){ //hom ref - genotype_component[1] = my_ensemble.diploid_choice[0]; - } - - loc_gt = convertToString(genotype_component[0]) + "/" + convertToString(genotype_component[1]); - loc_gq = int(round(evaluated_genotype_quality)); // genotype quality is rounded as an integer. - // append inference_results_union - inference_results_union.push_back({loc_min_allele_freq, loc_qual, loc_gt, loc_gq}); - } - else{ // We've seen loc_min_allele_freq before. Don't need to call CallGermline(...) again. - loc_qual = it->qual; - loc_gq = it->gq; - loc_gt = it->gt; - } - - // write the info tag for the corresponding var type - candidate_variant.variant.samples[sample_name][type_prefix + "MUAF"].push_back(convertToString(loc_min_allele_freq)); - candidate_variant.variant.samples[sample_name][type_prefix + "MUQUAL"].push_back(convertToString(loc_qual)); - candidate_variant.variant.samples[sample_name][type_prefix + "MUGT"].push_back(loc_gt); - candidate_variant.variant.samples[sample_name][type_prefix + "MUGQ"].push_back(convertToString(loc_gq)); - - switch(type_prefix[0]){ - case 'S': - is_snp_done = true; - break; - case 'M': - is_mnp_done = true; - break; - case 'I': - is_indel_done = true; - break; - case 'H': - is_hotspot_done = true; - break; - } - } - } - // sort inference_results_union according to min_allele_freq in the ascending order - sort(inference_results_union.begin(), inference_results_union.end()); - // write the info tag for the union of min_allele_freq of the var types - for(unsigned int i_freq = 0; i_freq < inference_results_union.size(); ++i_freq){ - candidate_variant.variant.samples[sample_name]["MUAF"].push_back(convertToString(inference_results_union[i_freq].min_allele_freq)); - candidate_variant.variant.samples[sample_name]["MUQUAL"].push_back(convertToString(inference_results_union[i_freq].qual)); - candidate_variant.variant.samples[sample_name]["MUGT"].push_back(inference_results_union[i_freq].gt); - candidate_variant.variant.samples[sample_name]["MUGQ"].push_back(convertToString(inference_results_union[i_freq].gq)); +void EnsembleEval::MultiMinAlleleFreq(const vector& multi_min_allele_freq){ + variant->info["MUQUAL"].clear(); + variant->info["MUGT"].clear(); + variant->info["MUGQ"].clear(); + for (vector::const_iterator maf_it = multi_min_allele_freq.begin(); maf_it != multi_min_allele_freq.end(); ++maf_it){ + float loc_qual = 0.0f; + int loc_gq = 0; + float evaluated_genotype_quality = 0.0f; + int quality_type = 0; + string loc_gt; + // Let's do the inference for min-allele-freq = *maf_it + vector genotype_component; + allele_eval.CallByIntegral(*maf_it, *maf_it, genotype_component, evaluated_genotype_quality, loc_qual, quality_type); + loc_gt = convertToString(genotype_component[0]) + "/" + convertToString(genotype_component[1]); + loc_gq = int(round(evaluated_genotype_quality)); // genotype quality is rounded as an integer. + variant->info["MUQUAL"].push_back(convertToString(loc_qual)); + variant->info["MUGT"].push_back(loc_gt); + variant->info["MUGQ"].push_back(convertToString(loc_gq)); } } +// Override fd-nonsnp-min-var-coverage +void OverrideMinVarCov(int fd_nonsnp_min_var_cov, + const vector& allele_identity_vector, + const vector >& global_flow_disruptive_matrix, + vector& variant_specific_params) +{ + assert(allele_identity_vector.size() + 1 == global_flow_disruptive_matrix.size()); + if (fd_nonsnp_min_var_cov < 1) + return; + + for (unsigned int i_alt = 0; i_alt < allele_identity_vector.size(); ++i_alt){ + // Override min_var_coverage of the allele if all the following are satisfied + // a) Not override by hotspot + // b) The allele is flow-disrupted + // c) It is not a SNP and it is not a padding SNP + if ((not variant_specific_params[i_alt].min_var_coverage_override) + and (global_flow_disruptive_matrix[0][i_alt + 1] == 2) + and (not (allele_identity_vector[i_alt].status.isSNP or allele_identity_vector[i_alt].status.isPaddedSNP))){ + variant_specific_params[i_alt].min_var_coverage_override = true; + variant_specific_params[i_alt].min_var_coverage = fd_nonsnp_min_var_cov; + } + } +} void GlueOutputVariant(EnsembleEval &my_ensemble, VariantCandidate &candidate_variant, const ExtendParameters ¶meters, int _best_allele_index, int sample_index){ - string sample_name = ""; - if(sample_index >= 0) { - sample_name = candidate_variant.variant.sampleNames[sample_index]; - } - + string sample_name = (sample_index >= 0) ? candidate_variant.variant.sampleNames[sample_index] : ""; DecisionTreeData my_decision(*(my_ensemble.variant)); + my_decision.use_molecular_tag = my_ensemble.allele_eval.total_theory.GetIsMolecularTag(); my_decision.tune_sbias = parameters.my_controls.sbias_tune; - my_decision.SetupFromMultiAllele(my_ensemble); + my_decision.SetupFromMultiAllele(&(my_ensemble.allele_identity_vector), &(my_ensemble.info_fields)); - // pretend we can classify reads across multiple alleles - if(not my_ensemble.is_hard_classification_for_reads_done_){ - my_ensemble.ApproximateHardClassifierForReads(); - } - - if(my_ensemble.allele_eval.total_theory.GetIsMolecularTag()){ - if(not my_ensemble.is_hard_classification_for_families_done_){ - my_ensemble.ApproximateHardClassifierForFamilies(); - } - // I count the strand coverage by families, not reads. - // Note that family_id_ is the hard classification results for functional and non-functional families. - // And we classify non-functional families as outliers. - // This gives FXX = 1.0 - (# of functional families) / (# of families). - my_decision.all_summary_stats.AssignStrandToHardClassifiedReads(my_ensemble.family_strand_id_, my_ensemble.family_id_); - } - else{ - // non-mol-tag - my_decision.all_summary_stats.AssignStrandToHardClassifiedReads(my_ensemble.strand_id_, my_ensemble.read_id_); + if (my_ensemble.read_id_.empty()){ + cerr << "ERROR: Can't GlueOutputVariant with empty read id." << endl; + exit(-1); } - + my_decision.all_summary_stats.AssignStrandToHardClassifiedReads(my_ensemble.strand_id_, my_ensemble.read_id_); my_decision.all_summary_stats.AssignPositionFromEndToHardClassifiedReads(my_ensemble.read_id_, my_ensemble.dist_to_left_, my_ensemble.dist_to_right_); - float smallest_allele_freq = 1.0f; - for (unsigned int _alt_allele_index = 0; _alt_allele_index < my_decision.allele_identity_vector.size(); _alt_allele_index++) { - // for each alt allele, do my best - // thresholds here can vary by >type< of allele - float local_min_allele_freq = FreqThresholdByType(my_ensemble.allele_identity_vector[_alt_allele_index], parameters.my_controls, - candidate_variant.variant_specific_params[_alt_allele_index]); - - if (local_min_allele_freq < smallest_allele_freq){ - smallest_allele_freq = local_min_allele_freq; // choose least-restrictive amongst multialleles - } - - /* The following piece of code seems redundant. Perhaps due to historical reasons? - my_ensemble.ComputePosteriorGenotype(_alt_allele_index, local_min_allele_freq, - my_decision.summary_info_vector[_alt_allele_index].genotype_call, - my_decision.summary_info_vector[_alt_allele_index].gt_quality_score, - my_decision.summary_info_vector[_alt_allele_index].variant_qual_score); - */ - SummarizeInfoFieldsFromEnsemble(my_ensemble, *(my_ensemble.variant), _alt_allele_index, sample_name); + for (unsigned int _alt_allele_index = 0; _alt_allele_index < my_decision.allele_identity_vector->size(); _alt_allele_index++) { + SummarizeInfoFieldsFromEnsemble(my_ensemble, *(my_ensemble.variant), _alt_allele_index, sample_name); } + if (my_ensemble.allele_eval.total_theory.GetIsMolecularTag()){ + my_ensemble.variant->info["TGSM"].clear(); + for (unsigned int _alt_allele_index = 0; _alt_allele_index < my_decision.allele_identity_vector->size(); _alt_allele_index++) { + if (my_ensemble.tag_similar_counts_.empty()){ + my_ensemble.variant->info["TGSM"].push_back("."); + }else{ + my_ensemble.variant->info["TGSM"].push_back(convertToString(my_ensemble.tag_similar_counts_[_alt_allele_index])); + } + } + my_decision.all_summary_stats.tag_similar_counts = my_ensemble.tag_similar_counts_; + } + my_decision.best_allele_index = _best_allele_index; my_decision.best_allele_set = true; + my_decision.is_possible_polyploidy_allele = my_ensemble.is_possible_polyploidy_allele; - // tell the evaluator to do a genotype - // choose a diploid genotype - // return it and set it so that decision tree cannot override + float af_cutoff_rej = 1.0f; + float af_cutoff_gt = 1.0f; + my_ensemble.ServeAfCutoff(parameters.my_controls, candidate_variant.variant_specific_params, af_cutoff_rej, af_cutoff_gt); - //@TODO: fix this frequency to be sensible - float local_min_allele_freq = smallest_allele_freq; // must choose a qual score relative to some frequency + if (my_ensemble.DEBUG > 0){ + cout << "+ Evaluating the variant (" << PrintVariant(*my_ensemble.variant) << "):" <size(); ++i_allele){ my_decision.summary_info_vector[i_allele].variant_qual_score = my_decision.eval_genotype.evaluated_variant_quality; my_decision.summary_info_vector[i_allele].gt_quality_score = my_decision.eval_genotype.evaluated_genotype_quality; } - // now that all the data has been gathered describing the variant, combine to produce the output - my_decision.DecisionTreeOutputToVariant(candidate_variant, parameters, sample_index); -} - -// Read and process records appropriate for this variant; positions are zero based -void EnsembleEval::StackUpOneVariant(const ExtendParameters ¶meters, const PositionInProgress& bam_position, int sample_index) -{ - - // Initialize random number generator for each stack -> ensure reproducibility - RandSchrange RandGen(parameters.my_controls.RandSeed); - - read_stack.clear(); // reset the stack - read_stack.reserve(parameters.my_controls.downSampleCoverage); - int read_counter = 0; - - for (Alignment* rai = bam_position.begin; rai != bam_position.end; rai = rai->next) { - - // Check global conditions to stop reading in more alignments - if (rai->original_position > multiallele_window_start) - break; - - if (rai->alignment.Position > multiallele_window_start) - continue; - - if (rai->filtered) - continue; - - if (rai->alignment.GetEndPosition() < multiallele_window_end) - continue; + // Override fd-nonsnp-min-var-coverage here + OverrideMinVarCov(parameters.my_controls.fd_nonsnp_min_var_cov, + my_ensemble.allele_identity_vector, + my_ensemble.global_flow_disruptive_matrix, + candidate_variant.variant_specific_params); - if (parameters.multisample) { - if (rai->sample_index != sample_index) { - continue; - } + if (parameters.my_controls.disable_filters){ + my_ensemble.GatherInfoForOfflineFiltering(parameters.my_controls, _best_allele_index); } - // Reservoir Sampling - if (read_stack.size() < (unsigned int)parameters.my_controls.downSampleCoverage) { - read_counter++; - read_stack.push_back(rai); - } else { - read_counter++; - // produces a uniformly distributed test_position between [0, read_counter-1] - unsigned int test_position = ((double)RandGen.Rand() / ((double)RandGen.RandMax + 1.0)) * (double)read_counter; - if (test_position < (unsigned int)parameters.my_controls.downSampleCoverage) - read_stack[test_position] = rai; - } - } + // now that all the data has been gathered describing the variant, combine to produce the output + my_decision.DecisionTreeOutputToVariant(candidate_variant, parameters, sample_index); } -// The class is simply used for random_shuffle -class MyRandSchrange : private RandSchrange{ -public: - MyRandSchrange(int seed = 1){SetSeed(seed);} ; - int operator()(int upper_lim){return Rand() % upper_lim;}; // return a random number between 0 and upper_lim-1 -}; - -// We do down sampling based on the following rules when we have molecular tag. -// Rule 1: Try to get as many functional families as possible after down sampling -// Rule 2: Try to equalize the sizes of functional families after down sampling. -// Rule 3: If we need to drop a functional family, drop the functional family with smaller family size. -void EnsembleEval::DoDownSamplingMolTag(const ExtendParameters ¶meters, vector< vector< MolecularFamily > > &my_molecular_families, - unsigned int num_reads_available, unsigned int num_func_fam, int strand_key) -{ - MyRandSchrange my_rand_schrange(parameters.my_controls.RandSeed); // The random number generator that we use to guarantee reproducibility. - unsigned int read_counter = 0; - unsigned int min_fam_size = (unsigned int) parameters.tag_trimmer_parameters.min_family_size; - unsigned int downSampleCoverage = (unsigned int) parameters.my_controls.downSampleCoverage; - unsigned int num_hyp = allele_identity_vector.size() + 2; - read_stack.clear(); // reset the stack - read_stack.reserve(downSampleCoverage); - allele_eval.total_theory.my_eval_families.clear(); - - // We can keep all the reads in all functional families :D - if(num_reads_available <= downSampleCoverage){ - allele_eval.total_theory.my_eval_families.reserve(num_reads_available); - for(vector< MolecularFamily >::iterator family_it = my_molecular_families[strand_key].begin(); - family_it != my_molecular_families[strand_key].end(); ++family_it){ - if(family_it->is_func_family_temp){ - allele_eval.total_theory.my_eval_families.push_back(EvalFamily(family_it->family_barcode, family_it->strand_key)); - allele_eval.total_theory.my_eval_families.back().InitializeEvalFamily(num_hyp); - for(unsigned int i_read = 0; i_read < family_it->family_members_temp.size(); ++i_read){ - read_stack.push_back(family_it->family_members_temp[i_read]); - allele_eval.total_theory.my_eval_families.back().AddNewMember(read_counter); - ++read_counter; - } - } - } - return; - } - - unsigned int num_of_func_fam_after_down_sampling = 0; - unsigned int min_fam_size_after_down_sampling = 0; - // The size of this vector is < 3, i.e., we only need to do random shuffle for the at most two different sizes of families. - // There are two cases that we need to randomly pick families. - // (random_shuffle case 1): We can not preserve the functional families of size = min_fam_size_after_down_sampling. - // (random_shuffle case 2): Some families of the same size have one more read than others. - vector size_of_fam_need_random_shuffle(0); - - // sort the families by the family size in the ascending order - sort(my_molecular_families[strand_key].begin(), my_molecular_families[strand_key].end()); - - // We need to give up some reads but we can keep all functional families :) - if(downSampleCoverage >= min_fam_size * num_func_fam){ - num_of_func_fam_after_down_sampling = num_func_fam; - unsigned int i_fam = my_molecular_families[strand_key].size() - num_of_func_fam_after_down_sampling; - min_fam_size_after_down_sampling = my_molecular_families[strand_key][i_fam].family_members_temp.size(); - } - // We need to give up some functional families... :( - else{ - num_of_func_fam_after_down_sampling = downSampleCoverage / min_fam_size; - unsigned int i_fam = my_molecular_families[strand_key].size() - num_of_func_fam_after_down_sampling; - min_fam_size_after_down_sampling = my_molecular_families[strand_key][i_fam].family_members_temp.size(); - if(i_fam < my_molecular_families[strand_key].size() - 1){ - if(min_fam_size_after_down_sampling == my_molecular_families[strand_key][i_fam + 1].family_members_temp.size()){ - // (random_shuffle case 1): - // We can't preserve all the families of size my_molecular_families[strand_key][i_fam].family_members_temp.size(). - // For fairness, we will to randomly pick some of families with this size. - size_of_fam_need_random_shuffle.push_back(my_molecular_families[strand_key][i_fam].family_members_temp.size()); - } - } - } - allele_eval.total_theory.my_eval_families.reserve(num_of_func_fam_after_down_sampling); - - // Count how many reads we want to pick in each family. - unsigned int reads_remaining = downSampleCoverage; - vector num_reads_picked_in_fam; - // num_reads_picked_in_fam[i] is the number of reads we picked for the family my_molecular_families[strand_key][i_fam] - // where i_fam = my_molecular_families[strand_key].size() - i - 1 - num_reads_picked_in_fam.assign(num_of_func_fam_after_down_sampling, 0); - - unsigned int break_at_i_fam = 0; - while(reads_remaining > 0){ - for(unsigned int i = 0; i < num_of_func_fam_after_down_sampling; ++i){ - unsigned int i_fam = my_molecular_families[strand_key].size() - i - 1; - // Note that my_molecular_families[strand_key][i_fam] should be functional since we sort my_molecular_families[strand_key] - // All the reads in the family are picked up, and of course for the next family since we sort my_molecular_families[strand_key] - // So let's break the for loop and starts with the family with the largest size. - if(num_reads_picked_in_fam[i] >= my_molecular_families[strand_key][i_fam].family_members_temp.size()){ - break; - } - - // We take one more read from the family - ++num_reads_picked_in_fam[i]; - --reads_remaining; - - // Sorry, we can't pick up more reads. We have reached the down sampling limit. - if(reads_remaining <= 0){ - break_at_i_fam = i_fam; - break; - } - } - } - - if(break_at_i_fam < my_molecular_families[strand_key].size() - 1){ - if(my_molecular_families[strand_key][break_at_i_fam].family_members_temp.size() == my_molecular_families[strand_key][break_at_i_fam + 1].family_members_temp.size()){ - // (random_shuffle case 2): - // Some of the families of size my_molecular_families[strand_key][break_at_i_fam].family_members_temp.size() get one more read after down sampling. - // For fairness, we will do random shuffle for the families of this size. - size_of_fam_need_random_shuffle.push_back(my_molecular_families[strand_key][break_at_i_fam].family_members_temp.size()); - } - } - - // sort size_of_fam_need_random_shuffle in increasing order and remove repeats - if(size_of_fam_need_random_shuffle.size() == 2){ - if(size_of_fam_need_random_shuffle[0] > size_of_fam_need_random_shuffle[1]){ - swap(size_of_fam_need_random_shuffle[0], size_of_fam_need_random_shuffle[1]); - } - else if(size_of_fam_need_random_shuffle[0] == size_of_fam_need_random_shuffle[1]){ - size_of_fam_need_random_shuffle.resize(1); - } +void RemoveVcfFormat(vcf::Variant &variant, const vector &keys){ + for(vector::const_iterator key_it = keys.begin(); key_it != keys.end(); ++key_it){ + variant.format.erase(std::remove(variant.format.begin(), variant.format.end(), *key_it), variant.format.end()); } +} - if(size_of_fam_need_random_shuffle.size() > 0){ - // Now we random shuffle the orders of the families of the same size to give more randomness if we can't pick all among them. - // Note that my_molecular_families[strand_key] will remain sorted after random_shuffle. - vector< MolecularFamily >::iterator current_fam_size_begin_it = my_molecular_families[strand_key].begin(); - for(vector< MolecularFamily >::iterator family_it = my_molecular_families[strand_key].begin(); - family_it != my_molecular_families[strand_key].end(); ++family_it){ - if(family_it->family_members_temp.size() != current_fam_size_begin_it->family_members_temp.size()){ - // we've got all families of size family_it->family_members_temp.size() - if(current_fam_size_begin_it->family_members_temp.size() == size_of_fam_need_random_shuffle[0]){ - // random shuffle the families of the size size_of_fam_need_random_shuffle[0] - random_shuffle(current_fam_size_begin_it, family_it, my_rand_schrange); - if(size_of_fam_need_random_shuffle.size() == 1){ - // We've done random shuffle for all families of the sizes needed - size_of_fam_need_random_shuffle.clear(); - break; - } - else{ - // size_of_fam_need_random_shuffle[0] is done. Will do random_shuffle for size_of_fam_need_random_shuffle[1] - // Let's do it like a sliding window. Note that size_of_fam_need_random_shuffle must be sorted as well!!! - size_of_fam_need_random_shuffle.assign(1, size_of_fam_need_random_shuffle[1]); - } - } - current_fam_size_begin_it = family_it; - } - } - // Don't forget to random shuffle the families if size_of_fam_need_random_shuffle[0] equals the maximum family size - if(size_of_fam_need_random_shuffle.size() > 0){ - random_shuffle(current_fam_size_begin_it, my_molecular_families[strand_key].end(), my_rand_schrange); - } - } - for(unsigned int i = 0; i < num_of_func_fam_after_down_sampling; ++i){ - unsigned int i_fam = my_molecular_families[strand_key].size() - i - 1; - MolecularFamily *ptr_fam = &(my_molecular_families[strand_key][i_fam]); - // Add one more family - allele_eval.total_theory.my_eval_families.push_back(EvalFamily(ptr_fam->family_barcode, ptr_fam->strand_key)); - allele_eval.total_theory.my_eval_families.back().InitializeEvalFamily(num_hyp); - allele_eval.total_theory.my_eval_families.back().family_members.reserve(num_reads_picked_in_fam[i]); - random_shuffle(ptr_fam->family_members_temp.begin(), ptr_fam->family_members_temp.end(), my_rand_schrange); - // Pick up num_reads_picked_in_fam[i] reads from family i_fam. - for(unsigned int i_read = 0; i_read < num_reads_picked_in_fam[i]; ++i_read){ - // Add the read into read_stack - read_stack.push_back(ptr_fam->family_members_temp[i_read]); - // Add the read in to the family - allele_eval.total_theory.my_eval_families.back().AddNewMember(read_counter); - ++read_counter; - } - } +void DoStepsForNoData(VariantCandidate& candidate_variant, const string& sample_name, int sample_index, bool use_molecular_tag, string my_reason){ + //cerr << "Nonfatal: No reads found for " << candidate_variant.variant.sequenceName << "\t" << my_ensemble.multiallele_window_start << endl; + NullFilterReason(candidate_variant.variant, sample_name); + if (my_reason.empty()){ + my_reason = "NODATA"; + } + AddFilterReason(candidate_variant.variant, my_reason, sample_name); + SetFilteredStatus(candidate_variant.variant, true); + candidate_variant.variant.samples[sample_name]["GT"] = {"./."}; } -// Currently only take the reads on one strand -void EnsembleEval::StackUpOneVariantMolTag(const ExtendParameters ¶meters, vector< vector< MolecularFamily > > &my_molecular_families, int sample_index) + +// return 0: normal termination +// return 1: no data (empty read stack) +// return 2: no data (no valid functional families on read stack) +int EnsembleProcessOneVariant(PersistingThreadObjects &thread_objects, VariantCallerContext& vc, + VariantCandidate &candidate_variant, const PositionInProgress& bam_position, + vector< vector > &molecular_families, int sample_index) { - int strand_key = -1; - unsigned int min_fam_size = (unsigned int) parameters.tag_trimmer_parameters.min_family_size; - vector num_func_fam_by_strand = {0, 0}; - vector num_reads_available_by_strand = {0, 0}; - - for(unsigned int i_strand = 0; i_strand < my_molecular_families.size(); ++i_strand){ - for(vector< MolecularFamily >::iterator family_it = my_molecular_families[i_strand].begin(); - family_it != my_molecular_families[i_strand].end(); ++family_it){ - if(not family_it->SetFunctionality(min_fam_size)){ - continue; - } - family_it->family_members_temp.reserve(family_it->family_members.size()); - for(vector::iterator member_it = family_it->family_members.begin(); member_it != family_it->family_members.end(); ++member_it){ - - // Although we have done this in the function GenerateMyMolecularFamilies, do it again to make sure everything is right. - if ((*member_it)->filtered) - continue; - // Although we have done this in the function GenerateMyMolecularFamilies, do it again to make sure everything is right. - if(parameters.multisample) { - if ((*member_it)->sample_index != sample_index) { - continue; - } - } - - // Check global conditions to stop reading in more alignments - if ((*member_it)->original_position > multiallele_window_start - or (*member_it)->alignment.Position > multiallele_window_start - or (*member_it)->alignment.GetEndPosition() < multiallele_window_end) - continue; - - - // family_members_temp stores the reads which are not filtered out here - family_it->family_members_temp.push_back((*member_it)); - } + unsigned long t0 = clock(); + string sample_name = (sample_index >= 0)? candidate_variant.variant.sampleNames[sample_index] : ""; + const bool use_molecular_tag = vc.mol_tag_manager->tag_trimmer->HaveTags(); - // We may change the family size since some reads may be filtered out. - // Need to determine the functionality again! - if(family_it->family_members_temp.size() >= min_fam_size){ - family_it->is_func_family_temp = true; - // Count how many reads and functional families available for down sampling - num_reads_available_by_strand[i_strand] += family_it->family_members_temp.size(); - ++num_func_fam_by_strand[i_strand]; - } - else{ - family_it->is_func_family_temp = false; - } - } - } + if(vc.parameters->program_flow.DEBUG > 0 ){ + cout<< endl << "[tvc] Start EnsembleProcessOneVariant for (" << PrintVariant(candidate_variant.variant) << ")"<< endl << endl; + } - // For the current molecular barcoding scheme (bcprimer), the reads in each amplicom should be on on strand only. - // However, we sometimes get families on both strands, primarily due to false priming. - // Here I pick the strand that has more functional families - strand_key = num_func_fam_by_strand[0] > num_func_fam_by_strand[1] ? 0 : 1; + if (not use_molecular_tag){ + RemoveVcfFormat(candidate_variant.variant, {"MDP", "MAO", "MRO", "MAF"}); + } - // Do down-sampling - DoDownSamplingMolTag(parameters, my_molecular_families, num_reads_available_by_strand[strand_key], num_func_fam_by_strand[strand_key], strand_key); -} + EnsembleEval my_ensemble(candidate_variant.variant); -bool EnsembleProcessOneVariant(PersistingThreadObjects &thread_objects, VariantCallerContext& vc, - VariantCandidate &candidate_variant, const PositionInProgress& bam_position, - vector< vector< MolecularFamily > > &molecular_families, int sample_index) -{ - string sample_name = ""; - bool use_molecular_tag = vc.tag_trimmer->HaveTags(); - if (sample_index >= 0) {sample_name = candidate_variant.variant.sampleNames[sample_index];} + // Allele preparation + my_ensemble.SetupAllAlleles(*vc.parameters, *vc.global_context, *vc.ref_reader); + my_ensemble.FilterAllAlleles(vc.parameters->my_controls, candidate_variant.variant_specific_params); // put filtering here in case we want to skip below entries - int chr_idx = vc.ref_reader->chr_idx(candidate_variant.variant.sequenceName.c_str()); + // set parameters for the evaluator + my_ensemble.SetAndPropagateParameters(vc.parameters, use_molecular_tag, candidate_variant.variant_specific_params); - EnsembleEval my_ensemble(candidate_variant.variant); - my_ensemble.allele_eval.total_theory.SetIsMolecularTag(use_molecular_tag); - my_ensemble.SetupAllAlleles(*vc.parameters, *vc.global_context, *vc.ref_reader, chr_idx); - my_ensemble.FilterAllAlleles(vc.parameters->my_controls.filter_variant, candidate_variant.variant_specific_params); // put filtering here in case we want to skip below entries + if (vc.parameters->program_flow.DEBUG > 0){ + list >allele_groups; + CandidateExaminer my_examiner(&thread_objects, &vc); + my_examiner.SetupVariantCandidate(candidate_variant); + my_examiner.FindLookAheadEnd0(); + my_examiner.SplitCandidateVariant(allele_groups); + } // We read in one stack per multi-allele variant - if(use_molecular_tag){ + if (use_molecular_tag){ my_ensemble.StackUpOneVariantMolTag(*vc.parameters, molecular_families, sample_index); } else{ my_ensemble.StackUpOneVariant(*vc.parameters, bam_position, sample_index); } + + // No data if (my_ensemble.read_stack.empty()) { - //cerr << "Nonfatal: No reads found for " << candidate_variant.variant.sequenceName << "\t" << my_ensemble.multiallele_window_start << endl; - NullFilterReason(candidate_variant.variant, sample_name); - if (not use_molecular_tag) { - RemoveVcfInfo(candidate_variant.variant, vector({"MDP", "MRO", "MAO", "MAF"}), sample_name, sample_index); - } - string my_reason = "NODATA"; - AddFilterReason(candidate_variant.variant, my_reason, sample_name); - SetFilteredStatus(candidate_variant.variant, true); - candidate_variant.variant.samples[sample_name]["GT"].clear(); - candidate_variant.variant.samples[sample_name]["GT"].push_back("./."); - return false; + DoStepsForNoData(candidate_variant, sample_name, sample_index, use_molecular_tag, "NODATA"); + if(vc.parameters->program_flow.DEBUG > 0 ){ + cout<< "+ No data: empty read stack!" << endl << endl + << "[tvc] Complete EnsembleProcessOneVariant for ("<< PrintVariant(candidate_variant.variant) << "). Processing time = " << (double) (clock() - t0) / 1E6 << " sec." << endl << endl; + } + return 1; } - - // handle the unfortunate case in which we must try multiple alleles to be happy - // try only ref vs alt allele here - // leave ensemble in ref vs alt state - // glue in variants - my_ensemble.SpliceAllelesIntoReads(thread_objects, *vc.global_context, *vc.parameters, *vc.ref_reader, chr_idx); + my_ensemble.SpliceAllelesIntoReads(thread_objects, *vc.global_context, *vc.parameters, *vc.ref_reader); - my_ensemble.allele_eval.my_params = vc.parameters->my_eval_control; + // Calculate flow-disruptiveness in the read level + my_ensemble.FlowDisruptivenessInReadLevel(*vc.global_context); // fill in quantities derived from predictions - int num_hyp_no_null = my_ensemble.allele_identity_vector.size()+1; // num alleles +1 for ref - my_ensemble.allele_eval.InitForInference(thread_objects, my_ensemble.read_stack, *vc.global_context, num_hyp_no_null, my_ensemble.allele_identity_vector); - - if(use_molecular_tag){ - // Filter out outlier reads to make sure there is no outlier family! - my_ensemble.allele_eval.total_theory.OutlierFiltering(vc.parameters->my_eval_control.DataReliability(), false); - my_ensemble.allele_eval.total_theory.SetFuncionalityForFamilies(vc.parameters->tag_trimmer_parameters.min_family_size); + my_ensemble.allele_eval.InitForInference(thread_objects, my_ensemble.read_stack, *vc.global_context, my_ensemble.allele_identity_vector); + + // No valid function family + if (use_molecular_tag){ + if (my_ensemble.allele_eval.total_theory.GetNumFuncFamilies() == 0) { + DoStepsForNoData(candidate_variant, sample_name, sample_index, use_molecular_tag, "NOVALIDFUNCFAM"); + if (vc.parameters->program_flow.DEBUG > 0){ + cout << "+ No valid functional families on read stack!" << endl << endl + << "[tvc] Complete EnsembleProcessOneVariant for ("<< PrintVariant(candidate_variant.variant) << "). Processing time = " << (double) (clock() - t0) / 1E6 << " sec." << endl << endl; + } + return 2; + } } + // do inference my_ensemble.allele_eval.ExecuteInference(); + + // set fd in the read_stack level. + my_ensemble.FlowDisruptivenessInReadStackLevel(vc.parameters->my_controls.min_ratio_for_fd); + // now we're in the guaranteed state of best index - int best_allele = my_ensemble.DetectBestMultiAllelePair(); + vector semi_soft_allele_freq_est; + int best_allele = my_ensemble.DetectBestMultiAllelePair(semi_soft_allele_freq_est); + if (vc.parameters->my_controls.report_ppa){ + my_ensemble.DetectPossiblePolyploidyAlleles(semi_soft_allele_freq_est, vc.parameters->my_controls, candidate_variant.variant_specific_params); + } + + if (use_molecular_tag){ + my_ensemble.CalculateTagSimilarity(*vc.mol_tag_manager, vc.parameters->my_controls.tag_sim_max_cov, sample_index); + my_ensemble.VariantFamilySizeHistogram(); + } // output to variant GlueOutputVariant(my_ensemble, candidate_variant, *vc.parameters, best_allele, sample_index); - if (not use_molecular_tag) { - RemoveVcfInfo(candidate_variant.variant, vector({"MDP", "MRO", "MAO", "MAF"}), sample_name, sample_index); + // output the inference results (MUQUAL, MUGT, MUGQ) if I turn on multi_min_allele_freq + if (vc.parameters->program_flow.is_multi_min_allele_freq){ + my_ensemble.MultiMinAlleleFreq(vc.parameters->program_flow.multi_min_allele_freq); } - // output the inference results (MUQUAL, MUGT, MUGQ, etc.) if I turn on multi_min_allele_freq - if(vc.parameters->program_flow.is_multi_min_allele_freq){ - MultiMinAlleleFreq(my_ensemble, candidate_variant, sample_index, vc.parameters->program_flow, vc.parameters->my_eval_control.max_detail_level); - } // test diagnostic output for this ensemble - if (vc.parameters->program_flow.rich_json_diagnostic & (!(my_ensemble.variant->isFiltered) | my_ensemble.variant->isHotSpot)) // look at everything that came through - JustOneDiagnosis(my_ensemble, *vc.global_context, vc.parameters->program_flow.json_plot_dir, true); - if (vc.parameters->program_flow.minimal_diagnostic & (!(my_ensemble.variant->isFiltered) | my_ensemble.variant->isHotSpot)) // look at everything that came through - JustOneDiagnosis(my_ensemble, *vc.global_context, vc.parameters->program_flow.json_plot_dir, false); + if (vc.parameters->program_flow.rich_json_diagnostic & (!(my_ensemble.variant->isFiltered) | my_ensemble.variant->isHotSpot)){ // look at everything that came through + cout << "+ Dumping rich json diagnostic for (" << PrintVariant(candidate_variant.variant) << ")" << endl; + JustOneDiagnosis(my_ensemble, *vc.global_context, vc.parameters->json_plot_dir, true); + } + if (vc.parameters->program_flow.minimal_diagnostic & (!(my_ensemble.variant->isFiltered) | my_ensemble.variant->isHotSpot)){ // look at everything that came through + cout << "+ Dumping minimal json diagnostic for (" << PrintVariant(candidate_variant.variant) << ")" << endl; + JustOneDiagnosis(my_ensemble, *vc.global_context, vc.parameters->json_plot_dir, false); + } - return true; + if(vc.parameters->program_flow.DEBUG > 0){ + cout << endl << "[tvc] Complete EnsembleProcessOneVariant for (" << PrintVariant(candidate_variant.variant) << "). Processing time = " << (double) (clock() - t0) / 1E6 << " sec." << endl << endl; + } + return 0; } +CandidateExaminer::CandidateExaminer(){ + thread_objects_ = NULL; + vc_ = NULL; + my_ensemble_ = NULL; + max_group_size_allowed_ = 32; +} +CandidateExaminer::CandidateExaminer(PersistingThreadObjects* thread_objects, VariantCallerContext* vc){ + thread_objects_ = NULL; + vc_ = NULL; + my_ensemble_ = NULL; + max_group_size_allowed_ = 32; + Initialize(thread_objects, vc); +} +CandidateExaminer::~CandidateExaminer(){ + ClearVariantCandidate(); +} +void CandidateExaminer::Initialize(PersistingThreadObjects* thread_objects, VariantCallerContext* vc){ + thread_objects_ = thread_objects; + vc_ = vc; + max_group_size_allowed_ = vc->parameters->max_alt_num; +} +// flow_disruptive_code[i][j] indicates the flow-disruptiveness between the j-th hyp (j=0: ref, j=1: allele 1, etc) and the query sequence of the read in test_read_stack[i] +// flow_disruptive_code[i][j] = -1: indefinite (fail to splicing, not cover the position, etc.) +// flow_disruptive_code[i][j] = 0: the j-th hyp is exactly the same as the query sequence +// flow_disruptive_code[i][j] = 1: the j-th hyp is an HP-INDEL of the query sequence +// flow_disruptive_code[i][j] = 2: the j-th hyp is neither HP-INDEL nor flow-disruptive of the query sequence +// flow_disruptive_code[i][j] = 3: the j-th hyp disrupts the flow of query sequence (i.e., the read is very unlikely to support the j-th hyp). +void CandidateExaminer::QuickExamFD(vector& test_read_stack, vector >& flow_disruptive_code) +{ + PersistingThreadObjects& thread_objects = *thread_objects_; + VariantCallerContext& vc = *vc_; + my_ensemble_->read_stack.swap(test_read_stack); + my_ensemble_->SpliceAllelesIntoReads(thread_objects, *vc.global_context, *vc.parameters, *vc.ref_reader); + my_ensemble_->allele_eval.total_theory.SetIsMolecularTag(false); + // Calculate flow-disruptiveness in the read level + my_ensemble_->FlowDisruptivenessInReadLevel(*vc.global_context); + flow_disruptive_code.resize(my_ensemble_->read_stack.size()); + vector >::iterator flow_disruptive_code_it = flow_disruptive_code.begin(); + int num_hyp_not_null = my_ensemble_->allele_identity_vector.size() + 1; + for (vector::iterator read_it = my_ensemble_->allele_eval.total_theory.my_hypotheses.begin(); read_it != my_ensemble_->allele_eval.total_theory.my_hypotheses.end(); ++read_it, ++flow_disruptive_code_it){ + flow_disruptive_code_it->assign(num_hyp_not_null, -1); + if (not read_it->success){ + continue; + } + for (int i_hyp = 0; i_hyp < num_hyp_not_null; ++i_hyp){ + if (read_it->same_as_null_hypothesis[i_hyp + 1]){ + flow_disruptive_code_it->at(i_hyp) = 0; + } + else if (read_it->local_flow_disruptiveness_matrix[0][i_hyp + 1] >= 0){ + flow_disruptive_code_it->at(i_hyp) = read_it->local_flow_disruptiveness_matrix[0][i_hyp + 1] + 1; + } + } + } + my_ensemble_->read_stack.swap(test_read_stack); +} + +// Setup the candidate variants for examination +// Minimal requirements of candidate_variant: +// a) candidate_variant.variant.sequenceName +// b) candidate_variant.variant.position +// c) candidate_variant.variant.ref +// d) candidate_variant.variant.alt +// e) candidate_variant.variant.isAltHotspot.size() == candidate_variant.variant.alt.size() +// f) candidate_variant.variant_specific_params.size() == candidate_variant.variant.alt.size() +void CandidateExaminer::SetupVariantCandidate(VariantCandidate& candidate_variant){ + if (my_ensemble_ == NULL){ + my_ensemble_ = new EnsembleEval(candidate_variant.variant); + } + else{ + *my_ensemble_ = EnsembleEval(candidate_variant.variant); + } + my_ensemble_->DEBUG = vc_->parameters->program_flow.DEBUG; + PrepareAlleles_(candidate_variant); +} + +void CandidateExaminer::ClearVariantCandidate(){ + if (my_ensemble_ != NULL){ + delete my_ensemble_; + } + my_ensemble_ = NULL; +} + +// Allele preparing/filtering steps +void CandidateExaminer::PrepareAlleles_(VariantCandidate& candidate_variant){ + my_ensemble_->SetupAllAlleles(*(vc_->parameters), *(vc_->global_context), *(vc_->ref_reader)); + my_ensemble_->FilterAllAlleles(vc_->parameters->my_controls, candidate_variant.variant_specific_params); // put filtering here in case we want to skip below entries +} + +// Given the candidate alleles, determine the maximally possible split of the variant (or group of the alternative alleles) that can be correctly (i.e., w/o high FXX) evaluated by the evaluator. +// e.g. output: allele_groups = {{0, 1, 2}, {3, 4}, {5}}. Then alt[0], alt[1], alt[2] must be evaluated jointly; alt[3], alt[4] must be evaluated jointly; alt[5] can be evaluated individually. +void CandidateExaminer::SplitCandidateVariant(list >& allele_groups){ + my_ensemble_->SplitMyAlleleIdentityVector(allele_groups, *(vc_->ref_reader), max_group_size_allowed_); +} + +// Given the variant candidates, calculate the end of the look ahead window for candidate generator, +// where (0-based) look ahead window = [last seen position + 1, look_ahead_end_0) +// I.e., the candidate generator should make sure that there is NO other de novo variant till the (0-based) position @ (look_ahead_end_0 - 1), while a variant @ look_ahead_end_0 is fine. +int CandidateExaminer::FindLookAheadEnd0(){ + int look_ahead_end_0 = my_ensemble_->CalculateLookAheadEnd0(*(vc_->ref_reader)); + return look_ahead_end_0; +} + +// return 1-based look ahead end +int CandidateExaminer::FindLookAheadEnd1(){ + return FindLookAheadEnd0() + 1; +} diff --git a/Analysis/VariantCaller/HandleVariant.h b/Analysis/VariantCaller/HandleVariant.h index d2358ec4..1c6cea1a 100644 --- a/Analysis/VariantCaller/HandleVariant.h +++ b/Analysis/VariantCaller/HandleVariant.h @@ -12,7 +12,6 @@ #include #include #include -#include #include #include #include @@ -29,35 +28,58 @@ #include "sys/types.h" #include "sys/stat.h" -#include #include #include #include #include - +#include +#include #include #include "InputStructures.h" -#include "MiscUtil.h" -#include "ExtendedReadInfo.h" -#include "ClassifyVariant.h" -#include "ExtendParameters.h" - -#include "StackEngine.h" -#include "ShortStack.h" -#include "DiagnosticJson.h" - using namespace std; using namespace BamTools; using namespace ion; +class MolecularFamily; +class EnsembleEval; // ---------------------------------------------------------------------- +// The function of doing flow-space evaluation for the variant +int EnsembleProcessOneVariant(PersistingThreadObjects &thread_objects, VariantCallerContext& vc, + VariantCandidate ¤t_variant, const PositionInProgress& bam_position, + vector< vector< MolecularFamily> > &molecular_families_one_sample, int sample_index = -1); +// ---------------------------------------------------------------------- +// The interface for the candidate generator to exam the candidate variants +class CandidateExaminer{ +private: + PersistingThreadObjects* thread_objects_; + VariantCallerContext* vc_; + EnsembleEval* my_ensemble_; + int max_group_size_allowed_; + //! @brief The allele preparation steps: setup alleles and filter alleles + void PrepareAlleles_(VariantCandidate& candidate_variant); -bool EnsembleProcessOneVariant(PersistingThreadObjects &thread_objects, VariantCallerContext& vc, - VariantCandidate ¤t_variant, const PositionInProgress& bam_position, - vector< vector< MolecularFamily > > &molecular_families_one_strand, int sample_index = -1); +public: + CandidateExaminer(); + CandidateExaminer(PersistingThreadObjects* thread_objects, VariantCallerContext* vc); + ~CandidateExaminer(); + //! @brief Initialize the object + void Initialize(PersistingThreadObjects* thread_objects, VariantCallerContext* vc); + //! @brief Setup variant candidates for examination + void SetupVariantCandidate(VariantCandidate& candidate_variant); + //! @brief Clear variant candidates + void ClearVariantCandidate(); + //! @brief Calculate the 0-based end position of the look ahead window + int FindLookAheadEnd0(); + //! @brief Calculate the 1-based end position of the look ahead window + int FindLookAheadEnd1(); + //! @brief Split the candidate variant into smaller callable variants + void SplitCandidateVariant(list >& allele_groups); + //! @brief Calculate the FD of the (reference, alternatives) alleles vs. reads as called on test_read_stack + void QuickExamFD(vector& test_read_stack, vector >& flow_disruptive_code); +}; #endif //HANDLEVARIANT_H diff --git a/Analysis/VariantCaller/HotspotReader.cpp b/Analysis/VariantCaller/HotspotReader.cpp index 172da20b..65326b9d 100644 --- a/Analysis/VariantCaller/HotspotReader.cpp +++ b/Analysis/VariantCaller/HotspotReader.cpp @@ -87,6 +87,7 @@ void HotspotReader::MakeHintQueue(const string& hotspot_vcf_filename) size_t r_pos = line.find("R", found+bstrand.size()); // look for the code F size_t f_pos = line.find("F", found+bstrand.size()); + size_t s_pos = line.find("S", found+bstrand.size()); bool blacklist = false; if (f_pos < semicolon_pos) { hint = FWD_BAD_HINT; @@ -98,8 +99,7 @@ void HotspotReader::MakeHintQueue(const string& hotspot_vcf_filename) hint = BOTH_BAD_HINT; } if ( (f_pos < semicolon_pos) && (r_pos < semicolon_pos)){ - hint = BOTH_BAD_HINT; - } + hint = BOTH_BAD_HINT; } // blacklist this position if (hint != NO_HINT) { @@ -110,11 +110,44 @@ void HotspotReader::MakeHintQueue(const string& hotspot_vcf_filename) char* end; // dummy variable for strtoll long int pos = strtoll(fields.at(1).c_str(), &end, 10); long int chrom_idx = ref_reader_->chr_idx(sequenceName.c_str()); - vector hint_entry (3, NO_HINT); - hint_entry[0] = chrom_idx; - hint_entry[1] = pos-1; - hint_entry[2] = hint; + hint_item hint_entry; + hint_entry.chr_ind = chrom_idx; + hint_entry.pos = pos-1; + hint_entry.value = hint; + hint_entry.rlen = 0; hint_vec.push_back(hint_entry); + } else if (s_pos < semicolon_pos) { + vector fields = split(line, '\t'); + string sequenceName = fields.at(0); + char* end; // dummy variable for strtoll + long int pos = strtoll(fields.at(1).c_str(), &end, 10); + long int chrom_idx = ref_reader_->chr_idx(sequenceName.c_str()); + vector alts = split(fields.at(4), ','); + vector bstr = split(line.substr(found+bstrand.size()+1, semicolon_pos-(found+bstrand.size()+1)), ','); + int len = fields.at(3).size(); + for (unsigned int i = 0; i < alts.size(); i++) { + if (bstr.at(i)[0] == 'S') { + hint = BOTH_BAD_HINT; + switch(bstr.at(i)[1]) { + case 'b': + hint = BOTH_BAD_HINT; + break; + case 'f': + hint = FWD_BAD_HINT; + break; + case 'r': + hint = REV_BAD_HINT; + break; + } + hint_item hint_entry; + hint_entry.chr_ind = chrom_idx; + hint_entry.pos = pos-1; + hint_entry.value = hint; + hint_entry.rlen = len; + hint_entry.alt = alts.at(i); + hint_vec.push_back(hint_entry); + } + } } } } @@ -156,6 +189,7 @@ void HotspotReader::FetchNextVariant() vector& strand_bias = current_hotspot.info["strand_bias"]; vector& min_coverage = current_hotspot.info["min_coverage"]; vector& min_coverage_each_strand = current_hotspot.info["min_coverage_each_strand"]; + vector& min_var_coverage = current_hotspot.info["min_var_coverage"]; vector& min_variant_score = current_hotspot.info["min_variant_score"]; vector& data_quality_stringency = current_hotspot.info["data_quality_stringency"]; vector& hp_max_length = current_hotspot.info["hp_max_length"]; @@ -163,6 +197,7 @@ void HotspotReader::FetchNextVariant() vector& filter_unusual_predictions = current_hotspot.info["filter_unusual_predictions"]; vector& filter_insertion_predictions = current_hotspot.info["filter_insertion_predictions"]; vector& filter_deletion_predictions = current_hotspot.info["filter_deletion_predictions"]; + vector& min_tag_fam_size = current_hotspot.info["min_tag_fam_size"]; vector& sse_prob_threshold = current_hotspot.info["sse_prob_threshold"]; // collect bad-strand info @@ -222,6 +257,11 @@ void HotspotReader::FetchNextVariant() hotspot.params.min_coverage_each_strand = atoi(min_coverage_each_strand[alt_idx].c_str()); } + if (alt_idx < min_var_coverage.size() and min_var_coverage[alt_idx] != ".") { + hotspot.params.min_var_coverage_override = true; + hotspot.params.min_var_coverage = atoi(min_var_coverage[alt_idx].c_str()); + } + if (alt_idx < min_variant_score.size() and min_variant_score[alt_idx] != ".") { hotspot.params.min_variant_score_override = true; hotspot.params.min_variant_score = atof(min_variant_score[alt_idx].c_str()); @@ -252,6 +292,11 @@ void HotspotReader::FetchNextVariant() hotspot.params.filter_deletion_predictions = atof(filter_deletion_predictions[alt_idx].c_str()); } + if (alt_idx < min_tag_fam_size.size() and min_tag_fam_size[alt_idx] != ".") { + hotspot.params.min_tag_fam_size_override = true; + hotspot.params.min_tag_fam_size = atof(min_tag_fam_size[alt_idx].c_str()); + } + if (alt_idx < sse_prob_threshold.size() and sse_prob_threshold[alt_idx] != ".") { hotspot.params.sse_prob_threshold_override = true; hotspot.params.sse_prob_threshold = atof(sse_prob_threshold[alt_idx].c_str()); diff --git a/Analysis/VariantCaller/HotspotReader.h b/Analysis/VariantCaller/HotspotReader.h index 5df3b2ad..3b3149d3 100644 --- a/Analysis/VariantCaller/HotspotReader.h +++ b/Analysis/VariantCaller/HotspotReader.h @@ -38,7 +38,16 @@ enum AlleleHint { REV_BAD_HINT = 2, BOTH_BAD_HINT = 3 }; - + +class hint_item { + public: + int chr_ind; + long int pos; + long int value; + long int rlen; + string alt; +}; + class HotspotReader { public: HotspotReader(); @@ -54,10 +63,12 @@ class HotspotReader { int next_chr() const { return next_chr_; } int next_pos() const { return next_pos_; } - vector< vector > hint_vec; - int hint_chr_index() const { return (int)hint_vec[hint_cur_][0]; } - long int hint_position() const { return hint_vec[hint_cur_][1]; } - long int hint_value() const { return hint_vec[hint_cur_][2]; } + vector< hint_item > hint_vec; + int hint_chr_index() const { return (int)hint_vec[hint_cur_].chr_ind; } + long int hint_position() const { return hint_vec[hint_cur_].pos; } + long int hint_value() const { return hint_vec[hint_cur_].value; } + long int hint_rlen() const { return hint_vec[hint_cur_].rlen; } + string hint_alt() const { return hint_vec[hint_cur_].alt; } bool hint_empty() const { return hint_vec.empty() || hint_header_ >= hint_vec.size();} void hint_pop() { hint_header_++;} void hint_next() { hint_cur_++;} diff --git a/Analysis/VariantCaller/IndelAssembly/IndelAssembly.cpp b/Analysis/VariantCaller/IndelAssembly/IndelAssembly.cpp index 9dfad168..6cc64ebb 100644 --- a/Analysis/VariantCaller/IndelAssembly/IndelAssembly.cpp +++ b/Analysis/VariantCaller/IndelAssembly/IndelAssembly.cpp @@ -1295,9 +1295,13 @@ void IndelAssemblyArgs::processParameters(OptArgs& opts) { } } - void IndelAssembly::onTraversalDone() { +// ----------------------------------------------------------------------------- +// The function takes a boolean signalling whether assembly output is desired + +void IndelAssembly::onTraversalDone(bool do_assembly) { + if (do_assembly) DetectCandidateRegions(WINDOW_SIZE); - out.close(); - } - \ No newline at end of file + out.close(); +} + diff --git a/Analysis/VariantCaller/IndelAssembly/IndelAssembly.h b/Analysis/VariantCaller/IndelAssembly/IndelAssembly.h index 1ded5374..33518485 100644 --- a/Analysis/VariantCaller/IndelAssembly/IndelAssembly.h +++ b/Analysis/VariantCaller/IndelAssembly/IndelAssembly.h @@ -249,7 +249,7 @@ class IndelAssembly { int getSoftStart(BamAlignment& alignment); void SetReferencePoint(BamAlignment& read); void map(BamAlignment& read); - void onTraversalDone(); + void onTraversalDone(bool do_assembly); void cleanCounts(); void shiftCounts(int delta); void DetectCandidateRegions(int wsize); diff --git a/Analysis/VariantCaller/IndelAssembly/IndelAssemblyMain.cpp b/Analysis/VariantCaller/IndelAssembly/IndelAssemblyMain.cpp index f095a79b..5b46381e 100644 --- a/Analysis/VariantCaller/IndelAssembly/IndelAssemblyMain.cpp +++ b/Analysis/VariantCaller/IndelAssembly/IndelAssemblyMain.cpp @@ -30,7 +30,7 @@ int main(int argc, char* argv[]) } SampleManager sample_manager; - sample_manager.Initialize(bam_reader.GetHeader(), parsed_opts.sample_name, parsed_opts.force_sample_name); + sample_manager.Initialize(bam_reader.GetHeader(), parsed_opts.sample_name, parsed_opts.force_sample_name, parsed_opts.multisample); IndelAssembly indel_assembly(&parsed_opts, &reference_reader, &sample_manager, &targets_manager); @@ -38,7 +38,7 @@ int main(int argc, char* argv[]) while (bam_reader.GetNextAlignment(alignment)) { if (!indel_assembly.processRead(alignment, indel_target)) {break;} } - indel_assembly.onTraversalDone(); + indel_assembly.onTraversalDone(true); bam_reader.Close(); diff --git a/Analysis/VariantCaller/MolecularTag.cpp b/Analysis/VariantCaller/MolecularTag.cpp index b7a3974c..a259f21b 100644 --- a/Analysis/VariantCaller/MolecularTag.cpp +++ b/Analysis/VariantCaller/MolecularTag.cpp @@ -3,10 +3,71 @@ #include "MolecularTag.h" #include "MiscUtil.h" +float median(vector& v) { + sort(v.begin(), v.end()); + int n = v.size(); + if (n == 0) {return 0;} + if ((n % 2) == 1) {return v[n/2];} + else {int i = n/2; return (v[i-1] + v[i])/2;} +} + +float mean(vector& v) { + unsigned int size = v.size(); + if (size == 0) {return 0;} + else { + float sum = 0; + for (vector::iterator iter = v.begin(); (iter != v.end()); ++iter) { + sum += *iter; + } + return (sum / size); + } +} + +int MolecularFamily::CountFamSizeFromAll() +{ + fam_size_ = 0; + for (vector::iterator read_it = all_family_members.begin(); read_it != all_family_members.end(); ++read_it){ + fam_size_ += ((*read_it)->read_count); + } + return fam_size_; +} + +int MolecularFamily::CountFamSizeFromValid() +{ + valid_fam_size_ = 0; + for (vector::iterator read_it = valid_family_members.begin(); read_it != valid_family_members.end(); ++read_it){ + valid_fam_size_ += ((*read_it)->read_count); + } + return valid_fam_size_; +} + +bool CompareByReadCounts(const Alignment* const rai_1, const Alignment* const rai_2){ + return rai_1->read_count > rai_2->read_count; +} + +void MolecularFamily::SortAllFamilyMembers(){ + sort(all_family_members.begin(), all_family_members.end(), CompareByReadCounts); + is_all_family_members_sorted = true; +} + +void MolecularFamily::SortValidFamilyMembers(){ + sort(valid_family_members.begin(), valid_family_members.end(), CompareByReadCounts); + is_valid_family_members_sorted = true; +} + +void MolecularFamily::ResetValidFamilyMembers(){ + AbstractMolecularFamily::ResetValidFamilyMembers(); + is_valid_family_members_sorted = false; +} + Consensus::Consensus() { min_start_position_ = 0; max_read_length_ = 0; debug_ = false; + flow_consensus_ = true; + error_ = false; + stitch_ = false; + iupac_cutoff_ = 1; } Consensus::~Consensus() { @@ -14,35 +75,57 @@ Consensus::~Consensus() { unsigned int Consensus::GetAlignedFamily(vector& family_members) { min_start_position_ = 999999999; + start_flow_ = 99999999; insertions_.clear(); aligned_bases_.clear(); insertion_bases_.clear(); + flow_indexes_.clear(); + measurement_vector_.clear(); + phase_params_.clear(); + flow_index_.clear(); + measurements_.clear(); + soft_clip_offset_.clear(); max_read_length_ = 0; - + error_ = false; + unsigned int map_quality = 0; for (vector::iterator iter = family_members.begin(); (iter != family_members.end()); ++iter) { + if (iter == family_members.begin()) {name_ = (*iter)->alignment.Name;} if (debug_) { + cerr << "Name " << (*iter)->alignment.Name << endl; cerr << "RefID:Position " << (*iter)->alignment.RefID << ":" << (*iter)->alignment.Position << endl; + cerr << "align_start " << (*iter)->align_start << endl; cerr << "start " << (*iter)->start << endl; cerr << "end " << (*iter)->end << endl; cerr << "QueryBases " << (*iter)->alignment.QueryBases << endl; cerr << "AlignedBases " << (*iter)->alignment.AlignedBases << endl; } + phase_params_.push_back((*iter)->phase_params); map_quality += (*iter)->alignment.MapQuality; min_start_position_ = min(min_start_position_, (unsigned int)((*iter)->alignment.Position)); unsigned int offset = 0; for (unsigned int position = min_start_position_; (position < (unsigned int)((*iter)->alignment.Position)); ++position) {offset++;} max_read_length_ = max(max_read_length_, (std::basic_string::size_type)(((*iter)->alignment.AlignedBases.length() + offset))); unsigned int pos = 0; + int soft_clip = 0; + unsigned int cigar_index = 0; for (vector::iterator iterCigar = (*iter)->alignment.CigarData.begin(); (iterCigar != (*iter)->alignment.CigarData.end()); ++iterCigar) { if (debug_) {cerr << iterCigar->Type << " " << iterCigar->Length << endl;} + if (iterCigar == (*iter)->alignment.CigarData.begin()) { + if (iterCigar->Type == 'S') { + soft_clip = iterCigar->Length; + } + } if (iterCigar->Type == 'I') { std::map::iterator iterInsertions = insertions_.find(pos + offset); if (iterInsertions == insertions_.end()) {insertions_[pos + offset] = iterCigar->Length;} else {insertions_[pos + offset] = max(iterInsertions->second, iterCigar->Length);} } if (iterCigar->Type == 'M') {pos += iterCigar->Length;} + if (iterCigar->Type == 'D') {pos += iterCigar->Length;} + cigar_index++; } + soft_clip_offset_.push_back(soft_clip); } map_quality /= family_members.size(); unsigned int count = 0; @@ -51,21 +134,63 @@ unsigned int Consensus::GetAlignedFamily(vector& family_members) { } max_read_length_ += count; insertion_bases_.assign(max_read_length_, '-'); + int read_index = -1; for (vector::iterator iter = family_members.begin(); (iter != family_members.end()); ++iter) { + read_index++; string str = (*iter)->alignment.AlignedBases; - str.reserve(max_read_length_); + if (flow_consensus_) { + flow_indexes_.push_back((*iter)->flow_index); + if (iter == family_members.begin()) { + flow_order_index_ = (*iter)->flow_order_index; + measurements_ = (*iter)->measurements; + for (vector::iterator init = measurements_.begin(); (init != measurements_.end()); ++init) {*init = 0;} + } + int start_flow = 0; + (*iter)->alignment.GetTag("ZF", start_flow); + start_flow_ = min(start_flow_, start_flow); + measurement_vector_.push_back((*iter)->measurements); + vector hp_counts = (*iter)->flow_index; + for (unsigned int index = 0; (index < (*iter)->flow_index.size()); ++index) { + if (index == (*iter)->flow_index.size() - 1) {hp_counts[index] = 1;} + else { + unsigned int index2 = index + 1; + for (; (index2 < (*iter)->flow_index.size()); ++index2) { + if ((*iter)->flow_index[index2] != (*iter)->flow_index[index]) {break;} + } + for (unsigned int index3 = index; (index3 < index2); ++index3) { + if (index3 == index) { + hp_counts[index3] = index2 - index; + } else { + hp_counts[index3] = 1; + } + } + index += index2 - index - 1; + } + } + int index = 0; + for (vector::iterator iter2 = (*iter)->flow_index.begin(); (iter2 != (*iter)->flow_index.end()); ++iter2) { + measurement_vector_[measurement_vector_.size() - 1][*iter2] /= hp_counts[index]; + index++; + } + } + str.reserve(max_read_length_); unsigned int insertion_count = 0; unsigned int offset = 0; - for (unsigned int position = min_start_position_; (position < (unsigned int)((*iter)->alignment.Position)); ++position) {str = '-' + str; insertion_count++; offset++;} - while (str.length() < max_read_length_) {str += "-";} + unsigned int length = 0; + str.reserve(max_read_length_); + char padding = '-'; + if (stitch_) {padding = ' ';} + for (unsigned int position = min_start_position_; (position < (unsigned int)((*iter)->alignment.Position)); ++position) {str = padding + str; insertion_count++; offset++; length++;} std::map read_insertions; unsigned int pos = 0; for (vector::iterator iterCigar = (*iter)->alignment.CigarData.begin(); (iterCigar != (*iter)->alignment.CigarData.end()); ++iterCigar) { if (iterCigar->Type == 'I') { - read_insertions[pos + offset] = iterCigar->Length; + read_insertions[pos + offset] = iterCigar->Length; length += iterCigar->Length; } - if (iterCigar->Type == 'M') {pos += iterCigar->Length;} + if (iterCigar->Type == 'M') {pos += iterCigar->Length; length += iterCigar->Length;} + if (iterCigar->Type == 'D') {pos += iterCigar->Length; length += iterCigar->Length;} } + while (str.length() < max_read_length_) {str += padding;} for (std::map::iterator iterInsertions = insertions_.begin(); (iterInsertions != insertions_.end()); ++iterInsertions) { for (unsigned int pos = iterInsertions->first + insertion_count - offset; (pos < (iterInsertions->first + insertion_count - offset + iterInsertions->second)); ++pos) { if (pos < insertion_bases_.length()) {insertion_bases_[pos] = '*';} @@ -83,8 +208,7 @@ unsigned int Consensus::GetAlignedFamily(vector& family_members) { insertion_count++; } str += part2; - } - else { + } else { string part1 = ""; if (iterInsertions->first + iterReadInsertions->second + insertion_count - offset < str.length()) {part1 = str.substr(0, iterInsertions->first + iterReadInsertions->second + insertion_count - offset);} string part2 = ""; @@ -96,12 +220,13 @@ unsigned int Consensus::GetAlignedFamily(vector& family_members) { } insertion_count += iterInsertions->second; str += part2; - + } } aligned_bases_.push_back(str); + read_counts_.push_back((*iter)->read_count); } - + max_read_length_ = 0; for (vector::iterator iter = aligned_bases_.begin(); (iter != aligned_bases_.end()); ++iter) { max_read_length_ = max(max_read_length_, (std::basic_string::size_type)(iter->length())); @@ -112,16 +237,25 @@ unsigned int Consensus::GetAlignedFamily(vector& family_members) { } void Consensus::GetConsensus(ReferenceReader& ref_reader, unsigned int RefID) { + // base space consensus consensus_ = ""; - consensus_.reserve(max_read_length_); + consensus_.reserve(max_read_length_); int A_count = 0; int C_count = 0; int G_count = 0; int T_count = 0; int N_count = 0; int D_count = 0; - int I_count = 0; + int I_count = 0; + int R_count = 0; + int Y_count = 0; + int S_count = 0; + int W_count = 0; + int K_count = 0; + int M_count = 0; + int total_count = 0; unsigned int position = min_start_position_; + while (insertion_bases_.length() < max_read_length_) {insertion_bases_ += '-';} for (unsigned int index = 0; (index < max_read_length_); ++index) { A_count = 0; C_count = 0; @@ -129,44 +263,167 @@ void Consensus::GetConsensus(ReferenceReader& ref_reader, unsigned int RefID) { T_count = 0; N_count = 0; D_count = 0; - I_count = 0; + I_count = 0; + R_count = 0; + Y_count = 0; + S_count = 0; + W_count = 0; + K_count = 0; + M_count = 0; + total_count = 0; + vector::iterator read_counts_iter = read_counts_.begin(); for (vector::iterator iter = aligned_bases_.begin(); (iter != aligned_bases_.end()); ++iter) { - if (index >= iter->length()) {D_count++;} + if (index >= iter->length()) {D_count+=(*read_counts_iter);} else { switch (iter->at(index)) { - case 'A': A_count++; break; - case 'C': C_count++; break; - case 'G': G_count++; break; - case 'T': T_count++; break; - case '-': D_count++; break; - case '*': I_count++; break; - default: N_count++; + case ' ': break; + case 'A': total_count+=(*read_counts_iter); A_count+=(*read_counts_iter); break; + case 'C': total_count+=(*read_counts_iter); C_count+=(*read_counts_iter); break; + case 'G': total_count+=(*read_counts_iter); G_count+=(*read_counts_iter); break; + case 'T': total_count+=(*read_counts_iter); T_count+=(*read_counts_iter); break; + case 'R': total_count+=(*read_counts_iter); R_count+=(*read_counts_iter); break; + case 'Y': total_count+=(*read_counts_iter); Y_count+=(*read_counts_iter); break; + case 'S': total_count+=(*read_counts_iter); S_count+=(*read_counts_iter); break; + case 'W': total_count+=(*read_counts_iter); W_count+=(*read_counts_iter); break; + case 'K': total_count+=(*read_counts_iter); K_count+=(*read_counts_iter); break; + case 'M': total_count+=(*read_counts_iter); M_count+=(*read_counts_iter); break; + case '-': total_count+=(*read_counts_iter); D_count+=(*read_counts_iter); break; + case '*': total_count+=(*read_counts_iter); I_count+=(*read_counts_iter); break; + default: total_count+=(*read_counts_iter); N_count+=(*read_counts_iter); } } + ++read_counts_iter; } + if (total_count == 0) {D_count = 1; total_count = 1;} char ref_base = 0; - if (insertion_bases_[index] != '*') {ref_base = (char)(*(ref_reader.iter(RefID, position)));} - int max_count = max(max(max(max(max(max(A_count, C_count), G_count), T_count), N_count), D_count), I_count); - if ((ref_base == 'G') and (max_count == G_count)) {C_count = 0; T_count = 0; A_count = 0; D_count = 0;} - else if ((ref_base == 'C') and (max_count == C_count)) {G_count = 0; T_count = 0; A_count = 0; D_count = 0;} - else if ((ref_base == 'T') and (max_count == T_count)) {C_count = 0; G_count = 0; A_count = 0; D_count = 0;} - else if ((ref_base == 'A') and (max_count == A_count)) {C_count = 0; G_count = 0; T_count = 0; D_count = 0;} - if (max_count == I_count) {consensus_ += '-';} - else if (max_count == G_count) {consensus_ += 'G';} - else if (max_count == C_count) {consensus_ += 'C';} - else if (max_count == T_count) {consensus_ += 'T';} - else if (max_count == A_count) {consensus_ += 'A';} - else if (max_count == D_count) {consensus_ += '-';} - else if (max_count == N_count) {consensus_ += 'N';} + if ((index < insertion_bases_.length()) and (insertion_bases_[index] != '*')) {ref_base = (char)(*(ref_reader.iter(RefID, position)));} + + int allele_count = 0; + float D_rate = (D_count / (double)total_count); + float A_rate = (A_count / (double)total_count); + float C_rate = (C_count / (double)total_count); + float G_rate = (G_count / (double)total_count); + float T_rate = (T_count / (double)total_count); + if (A_rate > iupac_cutoff_) {allele_count++;} + if (C_rate > iupac_cutoff_) {allele_count++;} + if (G_rate > iupac_cutoff_) {allele_count++;} + if (T_rate > iupac_cutoff_) {allele_count++;} + if ((allele_count == 1) and (D_rate > iupac_cutoff_)) {D_count = 0;} + if (allele_count > 1) { + if (debug_) { + cerr << A_rate << " " << C_rate << " " << G_rate << " " << T_rate << " " << D_rate << endl; + } + if ((A_rate > iupac_cutoff_) and (G_rate > iupac_cutoff_)) { + //consensus_ += "R"; + if (ref_base == 'A') { consensus_ += "G"; } else { consensus_ += "A"; } + } else if ((C_rate > iupac_cutoff_) and (T_rate > iupac_cutoff_)) { + //consensus_ += "Y"; + if (ref_base == 'C') { consensus_ += "T"; } else { consensus_ += "C"; } + } else if ((G_rate > iupac_cutoff_) and (C_rate > iupac_cutoff_)) { + //consensus_ += "S"; + if (ref_base == 'G') { consensus_ += "C"; } else { consensus_ += "G"; } + } else if ((A_rate > iupac_cutoff_) and (T_rate > iupac_cutoff_)) { + //consensus_ += "W"; + if (ref_base == 'A') { consensus_ += "T"; } else { consensus_ += "A"; } + } else if ((G_rate > iupac_cutoff_) and (T_rate > iupac_cutoff_)) { + //consensus_ += "K"; + if (ref_base == 'G') { consensus_ += "T"; } else { consensus_ += "G"; } + } else if ((A_rate > iupac_cutoff_) and (C_rate > iupac_cutoff_)) { + //consensus_ += "M"; + if (ref_base == 'A') { consensus_ += "C"; } else { consensus_ += "A"; } + } else {consensus_ += "N";} + } else { + int max_count = max(max(max(max(max(max(max(max(max(max(max(max(A_count, C_count), G_count), T_count), N_count), D_count), I_count), R_count), Y_count), S_count), W_count), K_count), M_count); + if ((ref_base == 'G') and (max_count == G_count)) {C_count = 0; T_count = 0; A_count = 0; D_count = 0;} + else if ((ref_base == 'C') and (max_count == C_count)) {G_count = 0; T_count = 0; A_count = 0; D_count = 0;} + else if ((ref_base == 'T') and (max_count == T_count)) {C_count = 0; G_count = 0; A_count = 0; D_count = 0;} + else if ((ref_base == 'A') and (max_count == A_count)) {C_count = 0; G_count = 0; T_count = 0; D_count = 0;} + if (max_count == I_count) {consensus_ += '-';} + else if (max_count == R_count) {consensus_ += 'R';} + else if (max_count == Y_count) {consensus_ += 'Y';} + else if (max_count == S_count) {consensus_ += 'S';} + else if (max_count == W_count) {consensus_ += 'W';} + else if (max_count == K_count) {consensus_ += 'K';} + else if (max_count == M_count) {consensus_ += 'M';} + else if (max_count == G_count) {consensus_ += 'G';} + else if (max_count == C_count) {consensus_ += 'C';} + else if (max_count == T_count) {consensus_ += 'T';} + else if (max_count == A_count) {consensus_ += 'A';} + else if (max_count == D_count) {consensus_ += '-';} + else if (max_count == N_count) {consensus_ += 'N';} + } if (insertion_bases_[index] != '*') {position++;} } + // flow space consensus + if (flow_consensus_) { + if (reverse_) { + RevComplementInPlace(consensus_); + for (vector::iterator iter = aligned_bases_.begin(); (iter != aligned_bases_.end()); ++iter) { + RevComplementInPlace(*iter); + } + } + vector positions; + unsigned int read_index = 0; + for (vector::iterator iter = aligned_bases_.begin(); (iter != aligned_bases_.end()); ++iter) {positions.push_back(soft_clip_offset_[read_index]); read_index++;} + // setup flow prefix + vector values; + values.reserve(flow_indexes_.size()); + unsigned int flow_index = start_flow_; + for (unsigned int flow = 0; (flow < (unsigned int)start_flow_); ++flow) { + values.clear(); + read_index = 0; + for (vector >::iterator iter = flow_indexes_.begin(); (iter != flow_indexes_.end()); ++iter) { + values.push_back(measurement_vector_[read_index][flow]); + read_index++; + } + if (flow >= measurements_.size()) {measurements_.resize(flow + 1);} + measurements_[flow] = median(values); + } + vector v; + v.reserve(flow_indexes_.size()); + flow_index_.reserve(max_read_length_); + for (unsigned int index = 0; (index < consensus_.length()); ++index) { + if (consensus_[index] != '-') { + while ((flow_index < flow_order_.length()) and (consensus_[index] != flow_order_[flow_index])) {flow_index++;} + if (flow_index >= flow_order_.length()) {consensus_ = consensus_.substr(0, index);} + else { + read_index = 0; + v.clear(); + for (vector >::iterator iter = flow_indexes_.begin(); (iter != flow_indexes_.end()); ++iter) { + if ((positions[read_index] < (*iter).size()) and (index < aligned_bases_[read_index].length())) { + if (aligned_bases_[read_index][index] == consensus_[index]) { + unsigned int flow = iter->at(positions[read_index]); + v.push_back(measurement_vector_[read_index][flow]); + } + } + read_index++; + } + flow_index_.push_back(flow_index); + measurements_[flow_index] += median(v); + } + } + read_index = 0; + for (vector::iterator iter = aligned_bases_.begin(); (iter != aligned_bases_.end()); ++iter) { + if (index < iter->length()) { + if ((iter->at(index) != ' ') and (iter->at(index) != '-') and (iter->at(index) != '*')) {positions[read_index]++;} + } + read_index++; + } + } + if (reverse_) { + RevComplementInPlace(consensus_); + for (vector::iterator iter = aligned_bases_.begin(); (iter != aligned_bases_.end()); ++iter) { + RevComplementInPlace(*iter); + } + } + } } unsigned int Consensus::TrimConsensus() { std::vector offsets; unsigned int insertion_count = 0; for (std::map::iterator iterInsertions = insertions_.begin(); (iterInsertions != insertions_.end()); ++iterInsertions) { - offsets.push_back(insertion_count); + offsets.push_back(insertion_count); insertion_count += iterInsertions->second; } new_insertions_.clear(); @@ -174,21 +431,23 @@ unsigned int Consensus::TrimConsensus() { int offset_index = insertions_.size(); for (std::map::reverse_iterator iterInsertions = insertions_.rbegin(); (iterInsertions != insertions_.rend()); ++iterInsertions) { offset_index--; - for (unsigned int pos = (min((unsigned int)consensus_.length(), iterInsertions->first + iterInsertions->second + offsets[offset_index]) - 1); (pos >= iterInsertions->first + offsets[offset_index]); --pos) { - if (consensus_[pos] == '-') { - if (pos == consensus_.length() - 1) {consensus_ = consensus_.substr(0, pos);} - else { - consensus_ = consensus_.substr(0, pos) + consensus_.substr(pos + 1); - } + for (int pos = (min((unsigned int)consensus_.length(), iterInsertions->first + iterInsertions->second + offsets[offset_index]) - 1); (pos >= (int)(iterInsertions->first + offsets[offset_index])); --pos) { + if (pos >= (int)consensus_.size()) {continue;} + if (pos < 0) {break;} + if (consensus_[pos] == '-') { + if (pos == (int)consensus_.length() - 1) {consensus_ = consensus_.substr(0, pos);} + else { + consensus_ = consensus_.substr(0, pos) + consensus_.substr(pos + 1); + } iterInsertions->second--; } } - } + } // trim begining of consensus unsigned int new_position = min_start_position_; unsigned int removed_count = 0; for (unsigned int index = 0; (index < consensus_.length()); ++index) { - if (index >= consensus_.length()) {break;} + if (index >= consensus_.length()) {break;} if (consensus_[index] == '-') { if (index + 1 >= consensus_.length()) {consensus_ = "";} else {consensus_ = consensus_.substr(index + 1);} @@ -196,8 +455,8 @@ unsigned int Consensus::TrimConsensus() { new_position++; index--; } else {break;} - } - // adjust insertion positions + } + // adjust insertion positions for (std::map::iterator iterInsertions = insertions_.begin(); (iterInsertions != insertions_.end()); ++iterInsertions) { if (iterInsertions->second != 0) {new_insertions_[iterInsertions->first - removed_count] = iterInsertions->second;} } @@ -223,12 +482,11 @@ void Consensus::CalculateCigar() { for (unsigned int index = 0; (index < (iterInsertions->first + offset - pos)); ++index) { if (pos + index >= consensus_.length()) {end = true; break;} if (consensus_[pos + index] == '-') { - deletion_count++; offset++; - } - else { + deletion_count++; + } else { if (deletion_count > 0) { if (m_count > 0) {new_cigar_.push_back(CigarOp('M', m_count)); m_count = 0;} - new_cigar_.push_back(CigarOp('D', deletion_count)); + new_cigar_.push_back(CigarOp('D', deletion_count)); deletion_count = 0; } m_count++; @@ -245,8 +503,7 @@ void Consensus::CalculateCigar() { if (pos + index >= consensus_.length()) {break;} if (consensus_[pos + index] == '-') { deletion_count++; - } - else { + } else { if (deletion_count > 0) { if (m_count > 0) {new_cigar_.push_back(CigarOp('M', m_count)); m_count = 0;} new_cigar_.push_back(CigarOp('D', deletion_count)); @@ -257,17 +514,76 @@ void Consensus::CalculateCigar() { } if (m_count > 0) {new_cigar_.push_back(CigarOp('M', m_count)); m_count = 0;} } +void Consensus::PartiallyResetAlignment_(Alignment& alignment) +{ + long int start = alignment.start; + long int end = alignment.end; + alignment.Reset(); + alignment.start = start; + alignment.end = end; +} + + +bool Consensus::CalculateConsensus(ReferenceReader& ref_reader, vector& family_members, Alignment& alignment, const string& flow_order) { + // Empty family_members + if (family_members.empty()) { + return false; + } + + // Trivial tie case + if (family_members.size() == 2){ + if (family_members[0]->read_count == family_members[1]->read_count){ + // I pick the read with the better mapping quality to be the consensus. + // In this case, I implicitly prefer to get the reference alleles. + alignment = (family_members[0]->alignment.MapQuality > family_members[1]->alignment.MapQuality)? *(family_members[0]) : *(family_members[1]); + alignment.read_count = family_members[0]->read_count + family_members[1]->read_count; + PartiallyResetAlignment_(alignment); + return true; + } + } + + int fam_size = 0; + int max_read_count = -1; + unsigned int max_read_count_idx = 0; + + // Calculate the family size and find the read w/ max read count. + for (unsigned int read_idx = 0; read_idx < family_members.size(); ++read_idx){ + int read_count = family_members[read_idx]->read_count; + fam_size += read_count; + if (read_count > max_read_count){ + max_read_count = read_count; + max_read_count_idx = read_idx; + } + } + // Check if family_members has a consensus read that dominates all the others. + // If true, I am actually calculating the consensus of consensus reads, which is trivial in this case. + // It should be the case most of the time if the input bam is a consensus bam. + if (2 * max_read_count >= fam_size){ + alignment = *(family_members[max_read_count_idx]); + alignment.read_count = fam_size; + PartiallyResetAlignment_(alignment); + return true; + } -bool Consensus::CalculateConsensus(ReferenceReader& ref_reader, vector& family_members, Alignment& alignment) { - if (family_members.size() < 1) {return false;} - alignment = *(family_members[0]); - //cerr << endl << family_barcode << " " << family_members.size() << " " << family_members[0]->alignment.RefID << " " << family_members[0]->alignment.Position << endl; - //debug_ = false; - //if ((family_members[0]->alignment.Position <= 55259524) and (family_members[0]->alignment.GetEndPosition() >= 55259524)) {debug_ = true;} - //if (alignment.alignment.Name == "TSJAM:02884:02345") {debug_ = true;} - //if ((family_barcode == "TTGACTTTGTGATCATAAAGTCAA") and (family_members.size() == 15) and (family_members[0]->alignment.Position == 29443576)) {debug_ = true;} + // If I didn't input flow_order, I can't generate flow consensus. So I go through the base space version instead. + bool flow_consensus_temp = flow_consensus_; + if (flow_order.empty()){ + SetFlowConsensus(false); + } + + flow_order_ = flow_order; + alignment = Alignment(*(family_members[0])); + alignment.read_count = fam_size; + PartiallyResetAlignment_(alignment); + reverse_ = alignment.is_reverse_strand; + debug_ = false; + bool show_variants = false; + int show_chr = 1; + long int show_pos = 2488153; + //if ((family_members[0]->alignment.RefID == (show_chr - 1)) and (family_members[0]->alignment.Position <= show_pos) and (family_members[0]->alignment.GetEndPosition() >= show_pos)) {show_variants = true;} + //if (alignment.alignment.Name == "7XBVF:02889:02037") {debug_ = true;} if (debug_) {cerr << endl << family_members.size() << " " << family_members[0]->alignment.RefID << " " << family_members[0]->alignment.Position << endl;} - + min_start_position_ = 0; insertions_.clear(); aligned_bases_.clear(); @@ -276,28 +592,33 @@ bool Consensus::CalculateConsensus(ReferenceReader& ref_reader, vector::iterator iterCigar = new_cigar_.begin(); (iterCigar != new_cigar_.end()); ++iterCigar) { if (iterCigar->Type == 'M') {end += iterCigar->Length;} } - + // output new consensus and cigar + alignment.flow_index = flow_index_; + alignment.measurements = measurements_; + alignment.read_bases = query_bases; if (debug_) { bool run_exit = false; cerr << endl; - cerr << alignment.alignment.Name << endl; + cerr << alignment.alignment.Name << endl; + + unsigned int index = 0; + for (vector::iterator iter = alignment.measurements.begin(); (iter != alignment.measurements.end()); ++iter) { + cerr << "measurements " << index << " " << *iter << endl; + index++; + } + vector hp_counts = alignment.flow_index; + for (index = 0; (index < alignment.flow_index.size()); ++index) { + if (index == alignment.flow_index.size() - 1) {hp_counts[index] = 1;} + else { + unsigned int index2 = index + 1; + for (; (index2 < alignment.flow_index.size()); ++index2) { + if (alignment.flow_index[index2] != alignment.flow_index[index]) {break;} + } + for (unsigned int index3 = index; (index3 < index2); ++index3) { + hp_counts[index3] = index2 - index; + } + index += index2 - index - 1; + } + } + index = 0; + for (vector::iterator iter = alignment.flow_index.begin(); (iter != alignment.flow_index.end()); ++iter) { + if (*iter < (int)flow_order_.length()) { + cerr << "flow_index " << index << " " << *iter << " " << alignment.measurements[*iter] << " " << alignment.read_bases[index] << " " << flow_order_[*iter] << " " << hp_counts[index] << " " << (alignment.measurements[*iter] / hp_counts[index]) << endl; + } + index++; + } + cerr << start << endl; cerr << end << endl; cerr << new_position << endl; @@ -323,130 +675,818 @@ bool Consensus::CalculateConsensus(ReferenceReader& ref_reader, vectorType << " " << iterCigar->Length << endl; if (iterCigar->Type == 'D') {run_exit = true;} } - //if (new_position == 115256481) {run_exit = true;} if (run_exit) { //exit(1); } - //cerr << "alignment " << alignment_time << endl; - //cerr << "consensus " << consensus_time << endl; - //cerr << "trim " << trim_time << endl; - //cerr << "cigar " << cigar_time << endl; - cerr << endl; - cerr << "Done" << endl; + cerr << endl; } - alignment.next = NULL; - alignment.processing_prev = NULL; - alignment.processing_next = NULL; alignment.start = start; alignment.end = end; alignment.alignment.Position = new_position; alignment.alignment.MapQuality = map_quality; alignment.alignment.QueryBases = query_bases; alignment.alignment.Length = query_bases.length(); + alignment.alignment.Qualities = "*"; + alignment.alignment.Length = query_bases.length(); alignment.alignment.AlignedBases = consensus_; - alignment.alignment.CigarData = new_cigar_; - alignment.refmap_start.clear(); - alignment.refmap_code.clear(); - alignment.refmap_has_allele.clear(); - alignment.refmap_allele.clear(); - alignment.read_count = family_members.size(); + alignment.alignment.CigarData.swap(new_cigar_); + + alignment.alignment.RemoveTag("NM"); + alignment.alignment.RemoveTag("XM"); + alignment.alignment.RemoveTag("MD"); + alignment.alignment.RemoveTag("ZF"); + alignment.alignment.RemoveTag("ZP"); + alignment.alignment.RemoveTag("ZM"); + alignment.alignment.RemoveTag("ZN"); + alignment.alignment.RemoveTag("ZR"); + // calculate ZM tag + vector measurements; + for (vector::iterator iter = measurements_.begin(); (iter != measurements_.end()); ++iter) { + measurements.push_back((int)(*iter * 256)); + } + // calculate MD tag + //bool snp = false; + string md = ""; + int match_count = 0; + int mismatch_count = 0; + int total_match_count = 0; + unsigned int ref_pos = alignment.start; + unsigned int seq_pos = alignment.start; + for (vector::iterator iterCigar = new_cigar_.begin(); (iterCigar != new_cigar_.end()); ++iterCigar) { + if (iterCigar->Type == 'M') { + for (unsigned int i = 0; (i < iterCigar->Length); ++i) { + if ((unsigned int)(seq_pos - alignment.start) < alignment.alignment.AlignedBases.length()) { + if (alignment.alignment.AlignedBases[seq_pos - alignment.start] == (char)(*(ref_reader.iter(alignment.alignment.RefID, ref_pos)))) { + match_count++; + total_match_count++; + } else { + //snp = true; + mismatch_count++; + if (match_count > 0) {md += std::to_string(match_count);} + md += alignment.alignment.AlignedBases[seq_pos - alignment.start]; + match_count = 0; + } + } + ref_pos++; + seq_pos++; + } + } else if (iterCigar->Type == 'D') { + for (unsigned int i = 0; (i < iterCigar->Length); ++i) { + if ((unsigned int)(seq_pos - alignment.start) < alignment.alignment.AlignedBases.length()) { + if (alignment.alignment.AlignedBases[seq_pos - alignment.start] == '-') { + if (match_count > 0) {md += std::to_string(match_count);} + md += "^"; + md += (char)(*(ref_reader.iter(alignment.alignment.RefID, ref_pos))); + match_count = 0; + } + } + ref_pos++; + seq_pos++; + } + } else if (iterCigar->Type == 'I') { + seq_pos += iterCigar->Length; + } + } + if (match_count > 0) {md += std::to_string(match_count);} + // calculate ZP tag + vector phase_params; + unsigned int max_size = 0; + for (vector >::iterator iter = phase_params_.begin(); (iter != phase_params_.end()); ++iter) { + if (iter->size() > max_size) {max_size = iter->size();} + } + vector values; + unsigned int index = 0; + while (index < max_size) { + values.clear(); + for (vector >::iterator iter = phase_params_.begin(); (iter != phase_params_.end()); ++iter) { + if (index < iter->size()) {values.push_back(iter->at(index));} + } + if (values.size() > 0) { + phase_params.push_back(mean(values)); + } + index++; + } + alignment.alignment.AddTag("NM", "i", mismatch_count); + alignment.alignment.AddTag("XM", "i", total_match_count); + alignment.alignment.AddTag("MD", "Z", md); + alignment.alignment.AddTag("ZF", "i", start_flow_); + alignment.alignment.AddTag("ZP", phase_params); + alignment.alignment.AddTag("ZM", measurements); + + // New tags "ZR", "ZN", "ZS" from consensus + /* + vec_temp.resize(measurements_sd.size()); + for (unsigned int i_flow = 0; i_flow < measurements_sd.size(); ++i_flow){ + vec_temp[i_flow] = (int16_t) (measurements_sd[i_flow] * 256.0f); + } + alignment.EditTag("ZS", vec_temp); + */ + alignment.alignment.AddTag("ZR", "i", (int) family_members.size()); // ZR tag = read count, number of reads that form the consensus read + string read_names = ""; + for (unsigned int i_member = 0; i_member < family_members.size(); ++i_member){ + read_names += family_members[i_member]->alignment.Name; + if (i_member != family_members.size() - 1){ + read_names += ";"; + } + } + for (string::iterator c_it = read_names.begin(); c_it != read_names.end(); ++c_it) + if (*c_it == ':') {*c_it = '.';} + alignment.alignment.AddTag("ZN", "Z", read_names); // ZN tag = query names of the reads that from the consensus read + + if (flow_consensus_) { + if (alignment.read_bases.length() != alignment.flow_index.size()) { + error_ = true; + cerr << "consensus " << alignment.alignment.Name << " read_bases.length() " << alignment.read_bases.length() << " flow_index.size() " << alignment.flow_index.size() << endl; + } else { + if (reverse_) {RevComplementInPlace(alignment.read_bases);} + unsigned int index = 0; + for (vector::iterator iter = alignment.flow_index.begin(); (iter != alignment.flow_index.end()); ++iter) { + if (((*iter) < (int)flow_order_.length()) and (alignment.read_bases[index] != flow_order_[*iter])) { + error_ = true; + cerr << "consensus " << alignment.alignment.Name << " base mismatch at " << index << endl; + break; + } + index++; + } + unsigned int flow = start_flow_; + unsigned int base_index = 0; + while (base_index < query_bases.length() and flow < flow_order_.length()) { + while (flow < flow_order_.length() and flow_order_[flow] != alignment.read_bases[base_index]) { + flow++; + } + if (flow >= flow_order_.length()) {break;} + base_index++; + } + if (reverse_) {RevComplementInPlace(alignment.read_bases);} + if (base_index != query_bases.length()) { + error_ = true; + cerr << "WARNING in MolecularTag::CalculateConsensus: There are more bases in the read than fit into the flow order.\t" << alignment.alignment.Name << endl; + cerr << query_bases << endl; + } + } + } + if (error_) {cerr << "Read(s) have flow errors. Consensus rejected.\t" << alignment.alignment.Name << endl;} + if ((not error_) and ((debug_) or (show_variants))) { + cerr << alignment.alignment.Name << " " << (alignment.is_reverse_strand ? "reverse" : "forward") << endl << endl; + string ref_seq = ""; + string allele = ""; + string ref = ""; + string gt = ""; + unsigned int pos = 0; + unsigned int ref_pos = alignment.start; + unsigned int seq_pos = alignment.start; + unsigned int variant_count = 0; + for (vector::iterator iterCigar = alignment.alignment.CigarData.begin(); (iterCigar != alignment.alignment.CigarData.end()); ++iterCigar) { + if (iterCigar->Type == 'M') { + for (unsigned int i = 0; (i < iterCigar->Length); ++i) { + ref = (char)(*(ref_reader.iter(alignment.alignment.RefID, ref_pos))); + if ((unsigned int)(seq_pos - alignment.start) < alignment.alignment.AlignedBases.length()) { + if (alignment.alignment.AlignedBases[seq_pos - alignment.start] != (char)(*(ref_reader.iter(alignment.alignment.RefID, ref_pos)))) { + pos = ref_pos; + allele = alignment.alignment.AlignedBases[seq_pos - alignment.start]; + gt = "0/1"; + if ((allele == "A") or (allele == "C") or (allele == "G") or (allele == "T")) {gt = "1/1";} + if ((allele == "R") and (ref == "A")) {allele = "G";} + else if ((allele == "R") and (ref == "G")) {allele = "A";} + else if (allele == "R") {allele = "A,G"; gt = "1/2";} + else if ((allele == "Y") and (ref == "C")) {allele = "T";} + else if ((allele == "Y") and (ref == "T")) {allele = "C";} + else if (allele == "Y") {allele = "C,T"; gt = "1/2";} + else if ((allele == "S") and (ref == "G")) {allele = "C";} + else if ((allele == "S") and (ref == "C")) {allele = "G";} + else if (allele == "S") {allele = "C,G"; gt = "1/2";} + else if ((allele == "W") and (ref == "A")) {allele = "T";} + else if ((allele == "W") and (ref == "T")) {allele = "A";} + else if (allele == "W") {allele = "A,T"; gt = "1/2";} + else if ((allele == "K") and (ref == "G")) {allele = "T";} + else if ((allele == "K") and (ref == "T")) {allele = "G";} + else if (allele == "K") {allele = "G,T"; gt = "1/2";} + else if ((allele == "M") and (ref == "A")) {allele = "C";} + else if ((allele == "M") and (ref == "C")) {allele = "A";} + else if (allele == "M") {allele = "A,C"; gt = "1/2";} + if (alignment.alignment.RefID + 1 == 23) { + cerr << "chrX" << "\t" << (pos + 1) << "\t" << "." << "\t" << (char)(*(ref_reader.iter(alignment.alignment.RefID, ref_pos))) << "\t" << allele << "\t" << 100 << "\t" << "PASS" << "\t" << "TYPE=snp" << "\t" << "GT" << "\t" << gt << ":" << endl; + } else { + cerr << "chr" << (alignment.alignment.RefID + 1) << "\t" << (pos + 1) << "\t" << "." << "\t" << (char)(*(ref_reader.iter(alignment.alignment.RefID, ref_pos))) << "\t" << allele << "\t" << 100 << "\t" << "PASS" << "\t" << "TYPE=snp" << "\t" << "GT" << "\t" << gt << ":" << endl; + } + std::transform(ref.begin(), ref.end(), ref.begin(), ::tolower); + variant_count++; + } + } + ref_seq += ref; + ref_pos++; + seq_pos++; + } + } else if (iterCigar->Type == 'D') { + pos = ref_pos - 1; + ref = (char)(*(ref_reader.iter(alignment.alignment.RefID, ref_pos - 1))); + allele = alignment.alignment.AlignedBases[seq_pos - alignment.start - 1]; + for (unsigned int i = 0; (i < iterCigar->Length); ++i) { + ref += (char)(*(ref_reader.iter(alignment.alignment.RefID, ref_pos))); + ref_seq += (char)(*(ref_reader.iter(alignment.alignment.RefID, ref_pos))); + ref_pos++; + seq_pos++; + } + if (alignment.alignment.RefID + 1 == 23) { + cerr << "chrX" << "\t" << (pos + 1) << "\t" << "." << "\t" << ref << "\t" << allele << "\t" << 100 << "\t" << "PASS" << "\t" << "TYPE=del" << "\t" << "GT" << "\t" << "0/1:" << endl; + } else { + cerr << "chr" << (alignment.alignment.RefID + 1) << "\t" << (pos + 1) << "\t" << "." << "\t" << ref << "\t" << allele << "\t" << 100 << "\t" << "PASS" << "\t" << "TYPE=del" << "\t" << "GT" << "\t" << "0/1:" << endl; + } + variant_count++; + } else if (iterCigar->Type == 'I') { + pos = ref_pos - 1; + allele = alignment.alignment.AlignedBases[seq_pos - alignment.start - 1]; + for (unsigned int i = 0; (i < iterCigar->Length); ++i) { + allele += alignment.alignment.AlignedBases[seq_pos - alignment.start]; + ref_seq += "-"; + seq_pos++; + } + if (alignment.alignment.RefID + 1 == 23) { + cerr << "chrX" << "\t" << (pos + 1) << "\t" << "." << "\t" << (char)(*(ref_reader.iter(alignment.alignment.RefID, pos))) << "\t" << allele << "\t" << 100 << "\t" << "PASS" << "\t" << "TYPE=ins" << "\t" << "GT" << "\t" << "0/1:" << endl; + } else { + cerr << "chr" << (alignment.alignment.RefID + 1) << "\t" << (pos + 1) << "\t" << "." << "\t" << (char)(*(ref_reader.iter(alignment.alignment.RefID, pos))) << "\t" << allele << "\t" << 100 << "\t" << "PASS" << "\t" << "TYPE=ins" << "\t" << "GT" << "\t" << "0/1:" << endl; + } + variant_count++; + } + } + cerr << endl; + for (vector::iterator iter = aligned_bases_.begin(); (iter != aligned_bases_.end()); ++iter) { + cerr << *iter << endl; + } + cerr << endl << ref_seq << endl; + cerr << alignment.alignment.AlignedBases << endl; + cerr << endl << "Done" << endl; + } + SetFlowConsensus(flow_consensus_temp); + return (not error_); +} + +void MolecularTagManager::Initialize(MolecularTagTrimmer* const input_tag_trimmer, const SampleManager* const sample_manager) +{ + tag_trimmer = input_tag_trimmer; + if (not tag_trimmer->HaveTags()){ + multisample_prefix_tag_struct_.clear(); + multisample_suffix_tag_struct_.clear(); + return; + } + multisample_prefix_tag_struct_.assign(sample_manager->num_samples_, ""); + multisample_suffix_tag_struct_.assign(sample_manager->num_samples_, ""); + cout << "MolecularTagManager: Found "<< tag_trimmer->NumTaggedReadGroups() << " read group(s) with molecular tags." << endl; + if (tag_trimmer->NumReadGroups() > tag_trimmer->NumTaggedReadGroups()){ + cerr << "Warning: MolecularTagManager: Molecular tags not found in read group(s) {"; + for (map::const_iterator rg_it = sample_manager->read_group_to_sample_idx_.begin(); rg_it != sample_manager->read_group_to_sample_idx_.end(); ++rg_it){ + const string& read_group = rg_it->first; + if (not tag_trimmer->HasTags(read_group)){ + cerr << read_group << ", "; + } + } + cerr << "}. The reads in these group(s) may be filtered out." << endl; + } + + // Check the uniqueness of tag structures cross the read groups in each sample. + for (map::const_iterator rg_it = sample_manager->read_group_to_sample_idx_.begin(); rg_it != sample_manager->read_group_to_sample_idx_.end(); ++rg_it){ + const int& sample_idx = rg_it->second; + const string& read_group = rg_it->first; + if (not tag_trimmer->HasTags(read_group)){ + // I implicitly allow a sample has a read group has tag but another read group doesn't. + continue; + } + if (multisample_prefix_tag_struct_[sample_idx].empty() and multisample_suffix_tag_struct_[sample_idx].empty()){ + multisample_prefix_tag_struct_[sample_idx] = tag_trimmer->GetPrefixTag(read_group); + multisample_suffix_tag_struct_[sample_idx] = tag_trimmer->GetSuffixTag(read_group); + } + else{ + if (multisample_prefix_tag_struct_[sample_idx] != tag_trimmer->GetPrefixTag(read_group) + or multisample_suffix_tag_struct_[sample_idx] != tag_trimmer->GetSuffixTag(read_group)){ + cerr << "ERROR: MolecularTagManager: Variable tag structures found in the sample " << sample_manager->sample_names_[sample_idx] << " !" << endl; + exit(-1); + return; + } + } + } + + for (unsigned int sample_idx = 0; sample_idx < sample_manager->sample_names_.size(); ++sample_idx){ + cout <<"MolecularTagManager: Found the unique molecular tag structures in the sample "<< sample_manager->sample_names_[sample_idx] << ": "; + cout <<"prefix tag = "<< (multisample_prefix_tag_struct_[sample_idx].empty()? "NULL" : multisample_prefix_tag_struct_[sample_idx]) << ", "; + cout <<"suffix tag = "<< (multisample_suffix_tag_struct_[sample_idx].empty()? "NULL" : multisample_suffix_tag_struct_[sample_idx]) << endl << endl; + + } +} + +bool IsStrictness(const string& mol_tag, const string& tag_struct) +{ + if ((mol_tag.size() != tag_struct.size())){ + return false; + } + + string::const_iterator tag_it = mol_tag.begin(); + for (string::const_iterator struct_it = tag_struct.begin(); struct_it != tag_struct.end(); ++struct_it){ + if ((*struct_it != 'N') and (*tag_it != *struct_it)){ + return false; + } + ++tag_it; + } return true; } +// return true if the tags match the tag structures. +bool MolecularTagManager::IsStrictTag(const string& prefix_tag, const string& suffix_tag, int sample_idx) const +{ + sample_idx = max(0, sample_idx); // sample_idx = -1 indicates no multisample + if (not IsStrictness(prefix_tag, multisample_prefix_tag_struct_[sample_idx])){ + return false; + } + if (not IsStrictness(suffix_tag, multisample_suffix_tag_struct_[sample_idx])){ + return false; + } + return true; +} + +// sample_idx = -1 indicates no multisample +bool MolecularTagManager::IsStrictSuffixTag(const string& suffix_tag, int sample_idx) const +{ + return IsStrictness(suffix_tag, multisample_suffix_tag_struct_[max(0, sample_idx)]); +} + +// sample_idx = -1 indicates no multisample +bool MolecularTagManager::IsStrictPrefixTag(const string& prefix_tag, int sample_idx) const +{ + return IsStrictness(prefix_tag, multisample_prefix_tag_struct_[max(0, sample_idx)]); +} + +MolecularTagManager::MolecularTagManager() +{ + multisample_prefix_tag_struct_.clear(); + multisample_suffix_tag_struct_.clear(); + tag_trimmer = NULL; +} + +// I stole the terms flat and run from Earl's indel assembly algorithm. a) flat = nuc of homopolymer, b) run = length of homopolymer +// Example: base_seq = "TAACGGG" gives flat_and_run = {('T', 1), ('A', 2), ('C', 1), ('G': 3)} +void BaseSeqToFlatAndRun(const string& base_seq, vector >& flat_and_run) +{ + flat_and_run.clear(); + if (base_seq.empty()){ + return; + } + flat_and_run.reserve(base_seq.size()); + flat_and_run.push_back(pair(base_seq[0], 1)); + for (int base_idx = 1; base_idx < (int) base_seq.size(); ++base_idx){ + if (base_seq[base_idx] == base_seq[base_idx - 1]){ + ++(flat_and_run.back().second); + }else{ + flat_and_run.push_back(pair(base_seq[base_idx], 1)); + } + } +} + +// Convert the vector of flat&run pair to the base sequence. +string FlatAndRunToBaseSeq(const vector >::const_iterator& it_begin, const vector >::const_iterator& it_end) +{ + string base_seq(""); + for (vector >::const_iterator it = it_begin; it != it_end; ++it){ + base_seq += string(it->second, it->first); + } + return base_seq; +} + +// Claim the two vectors of flat&run pairs synchronized if the following two criteria are both satisfied +// a) they have the same flat (shrank to the smaller length) +// b) the discrepancy between the runs <= allowed_hp_indel_len. +bool IsSyncFlatAndRun(const vector >::const_iterator it_1_begin, + const vector >::const_iterator it_1_end, + const vector >::const_iterator it_2_begin, + const vector >::const_iterator it_2_end, + int allowed_hp_indel_len) +{ + vector >::const_iterator it_1 = it_1_begin; + vector >::const_iterator it_2 = it_2_begin; + + while (it_1 != it_1_end and it_2 != it_2_end){ + if (it_1->first != it_2->first + or abs(it_1->second - it_2->second) > allowed_hp_indel_len){ + return false; + } + ++it_1; + ++it_2; + } + return true; +} + +bool MolecularTagManager::IsFlowSynchronizedTags(string tag_1, string tag_2, bool is_prefix) const +{ + if (tag_1.size() != tag_2.size()){ + return false; + } + + if (not is_prefix){ + // Be consistent with the tag trimming direction in BaseCaller. + reverse(tag_1.begin(), tag_1.end()); + reverse(tag_2.begin(), tag_2.end()); + } + int allowed_hp_indel_len = 1; + vector > flat_and_run_1; + vector > flat_and_run_2; + BaseSeqToFlatAndRun(tag_1, flat_and_run_1); + BaseSeqToFlatAndRun(tag_2, flat_and_run_2); + return IsSyncFlatAndRun(flat_and_run_1.begin(), flat_and_run_1.end(), flat_and_run_2.begin(), flat_and_run_2.end(), allowed_hp_indel_len); +} + +// Determine whether 2 tags are "partial similar" in the sense that the molecular tag may suffer from PCR error and sequencing error. +// By adjusting the length of the homopolymers appropriately, if the delta between the two tags is nothing or just "one" SNP, then I claim they are similar. +bool MolecularTagManager::IsPartialSimilarTags(string tag_1, string tag_2, bool is_prefix) const +{ + if (tag_1.size() != tag_2.size()){ + return false; + } + + if (not is_prefix){ + // Be consistent with the tag trimming direction in BaseCaller. + reverse(tag_1.begin(), tag_1.end()); + reverse(tag_2.begin(), tag_2.end()); + } -long long int NucToLongLong(char nuc) { - if (nuc=='A' or nuc=='a') return 0; - if (nuc=='C' or nuc=='c') return 1; - if (nuc=='G' or nuc=='g') return 2; - if (nuc=='T' or nuc=='t') return 3; - return -1; + // I allow "one" SNP and (allowed_hp_indel_len)-mer HP-INDELs. + int allowed_hp_indel_len = 1; + const static vector kAllNucs = {"T", "A", "C", "G"}; + vector > flat_and_run_1; + vector > flat_and_run_2; + BaseSeqToFlatAndRun(tag_1, flat_and_run_1); + BaseSeqToFlatAndRun(tag_2, flat_and_run_2); + vector >::iterator it_1 = flat_and_run_1.begin(); + vector >::iterator it_2 = flat_and_run_2.begin(); + bool is_not_begin = false; + + while (it_1 != flat_and_run_1.end() and it_2 != flat_and_run_2.end()){ + // Is "flat" the same? + if (it_1->first == it_2->first){ + if (is_not_begin){ + // For similar tags, every large HP-INDEL must be explained by a SNP. Otherwise, I claim not similar. + if (abs((it_1 - 1)->second - (it_2 - 1)->second) > allowed_hp_indel_len){ + return false; + } + } + ++it_1; + ++it_2; + }else{ + // Now the "flats" of the two tag are different. + // Since I only allow one SNP, the brute-force algorithm can be very efficient. + // Let's try all 6 possible combinations for altering the first base of the homopolymer at the flats. See if I can obtain two synchronized vectors of flat&run pairs. + + // Set the anchors + string tag_1_anchor = ""; + string tag_2_anchor = ""; + vector >::iterator anchor_it_1 = it_1; + vector >::iterator anchor_it_2 = it_2; + if (is_not_begin){ + --anchor_it_1; + --anchor_it_2; + tag_1_anchor = string(anchor_it_1->second, anchor_it_1->first); + tag_2_anchor = string(anchor_it_2->second, anchor_it_2->first); + } + + // Set the paddings + --(it_1->second); + --(it_2->second); + string tag_1_padding = FlatAndRunToBaseSeq(it_1, flat_and_run_1.end()); + string tag_2_padding = FlatAndRunToBaseSeq(it_2, flat_and_run_2.end()); + ++(it_1->second); + ++(it_2->second); + + for (int try_nuc_idx = 0; try_nuc_idx < 4; ++try_nuc_idx){ + if (kAllNucs[try_nuc_idx][0] != it_1->first){ + // Let's alter one base (where the base is the first base of the flat&run pair *it_1) of tag_1 to kAllNucs[try_nuc_idx][0] + string try_base_seq_1 = tag_1_anchor + kAllNucs[try_nuc_idx] + tag_1_padding; + vector > try_flat_and_run_1; + BaseSeqToFlatAndRun(try_base_seq_1, try_flat_and_run_1); + if (IsSyncFlatAndRun(try_flat_and_run_1.begin(), try_flat_and_run_1.end(), anchor_it_2 ,flat_and_run_2.end(), allowed_hp_indel_len)){ + return true; + } + } + if (kAllNucs[try_nuc_idx][0] != it_2->first){ + // Let's alter one base (where the base is the first base of the flat&run pair *it_2) of tag_2 to kAllNucs[try_nuc_idx][0] + string try_base_seq_2 = tag_2_anchor + kAllNucs[try_nuc_idx] + tag_2_padding; + vector > try_flat_and_run_2; + BaseSeqToFlatAndRun(try_base_seq_2, try_flat_and_run_2); + if (IsSyncFlatAndRun(try_flat_and_run_2.begin(), try_flat_and_run_2.end(), anchor_it_1, flat_and_run_1.end(), allowed_hp_indel_len)){ + return true; + } + } + } + // One SNP + HP-INDELs can't explain the delta between the two tags. Thus I claim not partial similar. + return false; + } + is_not_begin = true; + } + return true; +} + + + +char MolecularFamilyGenerator::NucTo0123_(char nuc) const +{ + if (nuc == 'A') { return 0; } + if (nuc == 'C') { return 1; } + if (nuc == 'G') { return 2; } + if (nuc == 'T') { return 3; } + if (nuc == 'a') { return 0; } + if (nuc == 'c') { return 1; } + if (nuc == 'g') { return 2; } + if (nuc == 't') { return 3; } + return -1; } -//@TODO: This can be done even faster by using memset -long long BarcodeStrToLongLong(const string &barcode_str){ - // I currently support the molecular barcode with total length < 32. - if(barcode_str.size() > 31){ - cerr<<"Warning: Length of the molecular barcode "<< barcode_str << " > 31 !"< 31){ + cerr << "ERROR: Cannot hash a string of char TACG of length >= 32 to a 64-bit long long integer." << endl; + exit(1); return -1; } - long long barcode_long_long = 0; - for(string::const_iterator it = barcode_str.begin(); it != barcode_str.end(); ++it){ - long long nuc_long_long = NucToLongLong(*it); - if(nuc_long_long < 0){ - return -1; // I see something not TACG - } - barcode_long_long <<= 2; - barcode_long_long |= nuc_long_long; - } - return barcode_long_long; -} - - -void GenerateMyMolecularFamilies(PositionInProgress &bam_position, - vector< vector< MolecularFamily > > &my_molecular_families, - const ExtendParameters ¶meters, - int sample_index){ - //MolecularTagClassifier mol_tag_classifier; - vector< map > barcode_lookup_table; // lookup table for barcodes - barcode_lookup_table.resize(2); - //mol_tag_classifier.PropagateTagParameters(parameters.my_mol_param); - // my_molecular_families[0] for fwd strand, my_molecular_families[1] for rev strand - my_molecular_families.clear(); - my_molecular_families.resize(2); - my_molecular_families[0].reserve(6000); // reserve 6000 families on each strand - my_molecular_families[1].reserve(6000); - - for(Alignment* rai = bam_position.begin; rai != bam_position.end; rai = rai->next){ - if (rai == NULL) {bam_position.end = NULL; break;} - // skip the read if it is not for this sample - if(parameters.multisample){ - if(rai->sample_index != sample_index){ - continue; - } - } - if(rai->filtered or (not rai->tag_info.HasTags())){ - continue; - } - - string barcode_of_read = rai->tag_info.prefix_mol_tag + rai->tag_info.suffix_mol_tag; - long long barcode_long_long = BarcodeStrToLongLong(barcode_of_read); - - if(barcode_long_long < 0){ - cerr<<"Warning: Skip the unsupported molecular barcode "<< barcode_of_read << " !"<< endl; - rai->filtered = true; + + long long base_seq_long_long = 0; + char* long_long_tail_byte = (char*) &base_seq_long_long; // pointer to the first byte of base_seq_long_long, like a bit mask. + bool non_valid_string = false; + for (string::const_iterator it = base_seq.begin(); it != base_seq.end(); ++it) { + char nuc_in_0123 = NucTo0123_(*it); + non_valid_string += (nuc_in_0123 < 0); + base_seq_long_long <<= 2; // shift 2 bits to the left + *long_long_tail_byte |= nuc_in_0123; // set the first 2 bits of barcode_long_long from the right to be nuc_in_0123 + } + if (non_valid_string){ + cerr << "ERROR: Invalid molecular tag that contains a non-TACG character." << endl; + exit(1); + return -1; + } + return base_seq_long_long; +} + +void MolecularFamilyGenerator::FindFamilyForOneRead_(Alignment* rai, vector< vector >& my_molecular_families) +{ + string mol_tag = rai->tag_info.prefix_mol_tag + rai->tag_info.suffix_mol_tag; + int strand_key = (rai->is_reverse_strand)? 1 : 0; + bool is_new_tag = true; + unsigned int tag_index_in_my_molecular_families = 0; + + // Hashing mol_tag to a long long integer facilitates the mapping between the mol_tag to the index of my_molecular_families. + // Note that the length of the mol_tag must be < 32. + if (long_long_hashable_){ + long long long_long_tag = BaseSeqToLongLong_(mol_tag); + pair< map::iterator, bool> tag_finder; + // map mol_tag_long_long to the index of my_family_[strand_key] for mol_tag + tag_finder = long_long_tag_lookup_table_[strand_key].insert(pair(long_long_tag, my_molecular_families[strand_key].size())); + // Note that map::insert will not insert value into the key of the map if key is pre-existed. + // tag_finder.first->first = mol_tag_long_long + // tag_finder.first->second = the index of my_molecular_families[strand_key] for tag + // tag_finder.second indicates inserted or not. + // tag_finder.second = false if I got mol_tag previously, and hence long_long_tag_lookup_table_ is not updated. + // tag_finder.second = true if this is the first time we get mol_tag, and hence mol_tag_long_long is inserted into long_long_tag_lookup_table_[strand_key] + + is_new_tag = tag_finder.second; + tag_index_in_my_molecular_families = tag_finder.first->second; + } + // Map a string to the index of my_molecular_families, slow but always safe. + else{ + pair< map::iterator, bool> tag_finder; + tag_finder = string_tag_lookup_table_[strand_key].insert(pair(mol_tag, my_molecular_families[strand_key].size())); + is_new_tag = tag_finder.second; + tag_index_in_my_molecular_families = tag_finder.first->second; + } + + if (is_new_tag){ + // Generate a new family since this is the first time I get the mol_tag + my_molecular_families[strand_key].push_back(MolecularFamily(mol_tag, strand_key)); + } + // Add the read to the family + my_molecular_families[strand_key][tag_index_in_my_molecular_families].AddNewMember(rai); +} + +// Generate molecular families +// Inputs: bam_position, sample_index, +// Output: my_molecular_families +// Set sample_index = -1 if not multi-sample. +void MolecularFamilyGenerator::GenerateMyMolecularFamilies(const MolecularTagManager* const mol_tag_manager, + PositionInProgress& bam_position, + int sample_index, + vector< vector >& my_molecular_families) +{ + bool is_consensus_bam = false; + unsigned int prefix_tag_len = (mol_tag_manager->GetPrefixTagStruct(sample_index)).size(); + unsigned int suffix_tag_len = (mol_tag_manager->GetSuffixTagStruct(sample_index)).size(); + long_long_hashable_ = (prefix_tag_len + suffix_tag_len) < 32; + + my_molecular_families.resize(2); // my_molecular_families[0] for fwd strand, my_molecular_families[1] for rev strand + long_long_tag_lookup_table_.resize(2); + string_tag_lookup_table_.resize(2); + + for (int i_strand = 0; i_strand < 2; ++i_strand){ + my_molecular_families[i_strand].resize(0); + my_molecular_families[i_strand].reserve(20000); // Reverse for 20000 families (including non-functional ones) per strand should be enough most of the time. + long_long_tag_lookup_table_[i_strand].clear(); + string_tag_lookup_table_[i_strand].clear(); + } + + for (Alignment* rai = bam_position.begin; rai != bam_position.end; rai = rai->next) { + if (rai == NULL) { + bam_position.end = NULL; + return; + } + + if (rai->filtered or (not rai->tag_info.HasTags())) { continue; - } - - int strand_key = (rai->is_reverse_strand)? 1 : 0; - - pair< map::iterator, bool> find_barcode; - // map barcode_of_read to the index of my_family_[strand_key] for barcode_of_read - find_barcode = barcode_lookup_table[strand_key].insert(pair(barcode_long_long, my_molecular_families[strand_key].size())); - // Note that - // find_barcode.first->first == barcode_of_read - // find_barcode.first->second is the index of my_family_[strand_key] for barcode_of_read - // find_barcode.second == true if we got barcode_of_read previously and hence we don't change barcode_lookup_table - // find_barcode.second == false if this is the first time we get barcode_of_read and we insert the barcode_lookup_table into barcode_lookup_table[strand_key] - - // Initialize if this is the first time we get the barcode - if(find_barcode.second){ - my_molecular_families[strand_key].push_back(MolecularFamily(barcode_of_read, strand_key)); - } - // add the read to the family - // note that find_barcode.first->second == barcode_lookup_table[strand_key][barcode_of_read] - my_molecular_families[strand_key][find_barcode.first->second].AddNewMember(rai); - } + } + + // skip the read if it is not for this sample + if( sample_index >=0 and rai->sample_index != sample_index) { + continue; + } + + // Tag length check + if ((rai->tag_info.prefix_mol_tag.size() > 0 and rai->tag_info.prefix_mol_tag.size() != prefix_tag_len) + or (rai->tag_info.suffix_mol_tag.size() > 0 and rai->tag_info.suffix_mol_tag.size() != suffix_tag_len)){ + cerr << "MolecularFamilyGenerator: Warning: The molecular tag length of the read "<< rai->alignment.Name << " doesn't match the tag structure provided in the bam header." << endl; + continue; + } + + // Skip the read whose tags don't exactly match the tag structures if I use strict trim. + // Note mol_tag_manager->tag_trimmer->tag_trim_method_ = 0, 1 indicates strict, sloppy, respectively. + if (mol_tag_manager->tag_trimmer->GetTagTrimMethod() == 0){ + if (not mol_tag_manager->IsStrictTag(rai->tag_info.prefix_mol_tag, rai->tag_info.suffix_mol_tag, sample_index)){ + continue; + } + } + + // If any read has read_count > 1 then I am dealing with a consensus bam + is_consensus_bam += (rai->read_count > 1); + FindFamilyForOneRead_(rai, my_molecular_families); + } + + if (is_split_families_by_region_){ + SplitFamiliesByRegion_(my_molecular_families); + } + + // Finally, sort the members in each family by the read counts if it is a consensus bam + for (vector >::iterator strand_it = my_molecular_families.begin(); strand_it != my_molecular_families.end(); ++strand_it){ + for (vector::iterator fam_it = strand_it->begin(); fam_it != strand_it->end(); ++fam_it){ + if (is_consensus_bam){ + fam_it->SortAllFamilyMembers(); + } + else{ + fam_it->is_all_family_members_sorted = true; // Sort no needed means sorted. + } + } + } +} + +// Split a family into multiple if the reads cover different target regions. +void MolecularFamilyGenerator::SplitFamiliesByRegion_(vector< vector >& my_molecular_families) const +{ + if (not is_split_families_by_region_){ + return; + } + for (vector >::iterator strand_it = my_molecular_families.begin(); strand_it != my_molecular_families.end(); ++strand_it){ + unsigned int original_fam_num_on_strand = strand_it->size(); + for (unsigned int i_fam = 0; i_fam < original_fam_num_on_strand; ++i_fam){ + if (strand_it->at(i_fam).all_family_members.size() < 2){ + continue; + } + bool need_split = false; + vector::iterator read_it_0 = strand_it->at(i_fam).all_family_members.begin(); + for (vector::iterator read_it = (read_it_0 + 1); read_it != strand_it->at(i_fam).all_family_members.end(); ++read_it){ + if ((*read_it)->target_coverage_indices != (*read_it_0)->target_coverage_indices){ + // Need to split the family if there is one read has a different target_coverage_indices. + need_split = true; + break; + } + } + if (need_split){ + vector splitted_families_from_one_family; + splitted_families_from_one_family.clear(); + for (vector::iterator read_it = strand_it->at(i_fam).all_family_members.begin(); read_it != strand_it->at(i_fam).all_family_members.end(); ++read_it){ + bool is_target_coverage_indices_exists = false; + vector::iterator splitted_fam_it = splitted_families_from_one_family.begin(); + for (; splitted_fam_it != splitted_families_from_one_family.end(); ++splitted_fam_it){ + if (splitted_fam_it->all_family_members[0]->target_coverage_indices == (*read_it)->target_coverage_indices){ + is_target_coverage_indices_exists = true; + break; + } + } + + if (is_target_coverage_indices_exists){ + splitted_fam_it->AddNewMember(*read_it); + }else{ + splitted_families_from_one_family.push_back(MolecularFamily(strand_it->at(i_fam).family_barcode, strand_it->at(i_fam).strand_key)); + splitted_families_from_one_family.back().AddNewMember(*read_it); + } + } + strand_it->at(i_fam).all_family_members.swap(splitted_families_from_one_family.begin()->all_family_members); + for (vector::iterator splitted_fam_it = splitted_families_from_one_family.begin() + 1; splitted_fam_it != splitted_families_from_one_family.end(); ++splitted_fam_it){ + strand_it->push_back(*splitted_fam_it); + } + } + } + } } -// Remove the non-functional families in the vector -void RemoveNonFuncFamilies(vector< MolecularFamily > &my_molecular_families, unsigned int min_fam_size){ - vector< MolecularFamily > molecular_families_temp; - molecular_families_temp.reserve(my_molecular_families.size()); - for(vector< MolecularFamily >::iterator fam_it = my_molecular_families.begin(); - fam_it != my_molecular_families.end(); ++fam_it){ - if(fam_it->SetFunctionality(min_fam_size)){ - molecular_families_temp.push_back(*fam_it); +/* +// Note that consensus_position_ticket must be generated from all samples! +// I.e., if one sample produces a candidate, then the candidate will be applied to all other samples as well. +void GenerateConsensusPositionTicket(vector< vector< vector > > &my_molecular_families_multisample, + VariantCallerContext &vc, + Consensus &consensus, + list::iterator &consensus_position_ticket, + bool filter_all_reads_after_done) { + unsigned int min_family_size = (unsigned int) vc.parameters->tag_trimmer_parameters.min_family_size; + for (vector< vector< vector< MolecularFamily> > >::iterator sample_it = my_molecular_families_multisample.begin(); sample_it != my_molecular_families_multisample.end(); ++sample_it) { + for (vector< vector< MolecularFamily> >::iterator strand_it = sample_it->begin(); strand_it != sample_it->end(); ++strand_it) { + for (vector< MolecularFamily>::iterator fam_it = strand_it->begin(); fam_it != strand_it->end(); ++fam_it) { + // Is *fam_it functional? + if (fam_it->SetFuncFromAll(min_family_size)) { + if (fam_it->all_family_members.size() >= 1) { + map > alignment_map; + for (vector::iterator iter = fam_it->all_family_members.begin(); (iter != fam_it->all_family_members.end()); ++iter) { + if (((*iter)->start > vc.bam_walker->getEndPosition()) or ((*iter)->end < vc.bam_walker->getStartPosition())) {continue;} + if ((*iter)->is_reverse_strand) { + alignment_map[1000000 + floor(((*iter)->end - vc.bam_walker->getEndPosition()) / 10.0)].push_back(*iter); + } else { + alignment_map[floor(((*iter)->start - vc.bam_walker->getStartPosition()) / 10.0)].push_back(*iter); + } + } + for (map >::iterator iter = alignment_map.begin(); (iter != alignment_map.end()); ++iter) { + Alignment* alignment = new Alignment; + const ion::FlowOrder & flow_order = vc.global_context->flow_order_vector.at(iter->second[0]->flow_order_index); + string flow_order_str; + flow_order_str.reserve(flow_order.num_flows()); + for (int i = 0; (i < flow_order.num_flows()); ++i) { + flow_order_str += flow_order.nuc_at(i); + } + bool success = true; + success = consensus.CalculateConsensus(*vc.ref_reader, iter->second, *alignment, flow_order_str); + if ((not success) or (not vc.candidate_generator->BasicFilters(*alignment))) { + delete alignment; + } else { + vc.targets_manager->TrimAmpliseqPrimers(alignment, vc.bam_walker->GetRecentUnmergedTarget()); + if (alignment->filtered) {delete alignment;} + else { + if (consensus_position_ticket->begin == NULL) {consensus_position_ticket->begin = alignment;} + if (consensus_position_ticket->end != NULL) {consensus_position_ticket->end->next = alignment;} + consensus_position_ticket->end = alignment; + } + } + } + } + } + if (filter_all_reads_after_done){ + for (vector::iterator read_it = fam_it->all_family_members.begin(); read_it != fam_it->all_family_members.end(); ++read_it) + (*read_it)->filtered = true; + } + } + } + } + consensus_position_ticket->end = NULL; +} +*/ + + +// Note that consensus_position_ticket must be generated from all samples! +// I.e., if one sample produces a candidate, then the candidate will be applied to all other samples as well. +void GenerateConsensusPositionTicket(vector< vector< vector > > &my_molecular_families_multisample, + VariantCallerContext &vc, + Consensus &consensus, + list::iterator &consensus_position_ticket, + bool filter_all_reads_after_done) +{ + unsigned int min_family_size = (unsigned int) vc.parameters->tag_trimmer_parameters.min_family_size; + for (vector< vector< vector< MolecularFamily> > >::iterator sample_it = my_molecular_families_multisample.begin(); sample_it != my_molecular_families_multisample.end(); ++sample_it) { + for (vector< vector< MolecularFamily> >::iterator strand_it = sample_it->begin(); strand_it != sample_it->end(); ++strand_it) { + for (vector< MolecularFamily>::iterator fam_it = strand_it->begin(); fam_it != strand_it->end(); ++fam_it) { + // Is *fam_it functional? + if (fam_it->SetFuncFromAll(min_family_size)) { + Alignment* alignment = new Alignment; + bool success = consensus.CalculateConsensus(*vc.ref_reader, fam_it->all_family_members, *alignment); + if ((not success) or (not vc.candidate_generator->BasicFilters(*alignment))) { + delete alignment; + } + else { + vc.targets_manager->TrimAmpliseqPrimers(alignment, vc.bam_walker->GetRecentUnmergedTarget()); + if (alignment->filtered) {delete alignment;} + else { + if (consensus_position_ticket->begin == NULL) {consensus_position_ticket->begin = alignment;} + if (consensus_position_ticket->end != NULL) {consensus_position_ticket->end->next = alignment;} + consensus_position_ticket->end = alignment; + } + } + if (filter_all_reads_after_done){ + for (vector::iterator read_it = fam_it->all_family_members.begin(); read_it != fam_it->all_family_members.end(); ++read_it) + (*read_it)->filtered = true; + } + } + } } } - my_molecular_families.swap(molecular_families_temp); + consensus_position_ticket->end = NULL; } diff --git a/Analysis/VariantCaller/MolecularTag.h b/Analysis/VariantCaller/MolecularTag.h index caaa4c6b..5bf729e8 100644 --- a/Analysis/VariantCaller/MolecularTag.h +++ b/Analysis/VariantCaller/MolecularTag.h @@ -10,85 +10,215 @@ #include #include #include +#include +#include +#include "InputStructures.h" #include "ExtendParameters.h" #include "BAMWalkerEngine.h" -#include +#include "AlleleParser.h" +#include "MolecularTagTrimmer.h" using namespace std; +class Timer { +private: + string tag_; + double run_time_; + timeval start_time_; + timeval end_time_; + pthread_mutex_t mutextimer_; +public: +Timer(const string& tag) {tag_ = tag; run_time_ = 0; pthread_mutex_init(&mutextimer_, NULL);} +virtual ~Timer() {print();} +void start() {gettimeofday(&start_time_, NULL);} +void end() { + gettimeofday(&end_time_, NULL); + pthread_mutex_lock (&mutextimer_); + run_time_ += ((end_time_.tv_sec - start_time_.tv_sec) + ((end_time_.tv_usec - start_time_.tv_usec) / 1000000.0)); + pthread_mutex_unlock (&mutextimer_); +} +void print() {cerr << tag_ << " run_time = " << run_time_ << " seconds." << endl;} +}; + class Consensus { private: + bool reverse_; + string name_; + string flow_order_; unsigned int min_start_position_; std::map insertions_; std::map new_insertions_; vector aligned_bases_; + vector read_counts_; + vector > flow_indexes_; + vector > measurement_vector_; + int start_flow_; + vector flow_index_; + vector measurements_; + vector > phase_params_; std::basic_string::size_type max_read_length_; string insertion_bases_; string consensus_; vector new_cigar_; bool debug_; - //double alignment_time = 0; - //double consensus_time = 0; - //double trim_time = 0; - //double cigar_time = 0; + bool flow_consensus_; + int flow_order_index_; + vector soft_clip_offset_; + bool error_; + bool stitch_; + float iupac_cutoff_; unsigned int GetAlignedFamily(vector& family_members); void GetConsensus(ReferenceReader& ref_reader, unsigned int RefID); unsigned int TrimConsensus(); void CalculateCigar(); - + void PartiallyResetAlignment_(Alignment& alignment); + public: Consensus(); virtual ~Consensus(); + void SetIUPACCutoff(float f) {iupac_cutoff_ = f;} + void SetStitch(bool b) {stitch_ = true;} + void SetFlowConsensus(bool b) {flow_consensus_ = b;} void SetDebug(bool b) {debug_ = b;} void GetAlignedBases(vector& v) {v = aligned_bases_;} void GetInsertionBases(string& str) {str = insertion_bases_;} void GetConsensus(string& str) {str = consensus_;} - - bool CalculateConsensus(ReferenceReader& ref_reader, vector& family_members, Alignment& alignment); + bool CalculateConsensus(ReferenceReader& ref_reader, vector& family_members, Alignment& alignment, const string& flow_order = ""); }; template -class MolecularFamily { +class AbstractMolecularFamily { public: int strand_key; string family_barcode; - vector family_members; - vector family_members_temp; // in case we want to apply additional rules to filter out some reads - Alignment consensus_alignment; - bool operator<(const MolecularFamily &rhs) const { return this->family_members_temp.size() < rhs.family_members_temp.size(); } // use for std::sort - bool is_func_family_temp; // is_func_family_temp indicates the functionality of this family if the family members are family_members_temp - - MolecularFamily(const string &barcode, int strand): family_barcode(barcode){ - strand_key = strand; - ResetFamily(); + vector all_family_members; // All reads identified in the family, no additional filtering applied. + vector valid_family_members; // The reads from all_family_members that pass certain filtering criterion. + + AbstractMolecularFamily (const string &barcode = "", int strand = -1) + : strand_key(strand), family_barcode(barcode) { all_family_members.reserve(4); }; + + virtual ~AbstractMolecularFamily(){}; + virtual int CountFamSizeFromAll() = 0; + virtual int CountFamSizeFromValid() = 0; + + bool SetFuncFromAll(unsigned int min_fam_size){ + if (fam_size_ < 0) {CountFamSizeFromAll(); } + is_func_from_family_members = (fam_size_ >= (int) min_fam_size); + return is_func_from_family_members; }; - void ResetFamily(){ - family_members.clear(); - family_members.reserve(16); - family_members_temp.clear(); - is_func_family_ = false; - is_func_family_temp = false; - consensus_alignment.Reset(); + bool SetFuncFromValid(unsigned int min_fam_size){ + if (valid_fam_size_ < 0) {CountFamSizeFromValid();} + is_func_from_valid_family_members = (valid_fam_size_ >= (int) min_fam_size); + return is_func_from_valid_family_members; }; - void AddNewMember(const MemberType &new_member) { family_members.push_back(new_member); }; - bool SetFunctionality(unsigned int min_fam_size){ - is_func_family_ = family_members.size() >= min_fam_size; - return is_func_family_; + + void AddNewMember(const MemberType& new_member) + { all_family_members.push_back(new_member); }; + + bool GetFuncFromAll() const { + assert(fam_size_ > -1); // CountFamSizeFromAll must be done first. + return is_func_from_family_members; }; - bool GetFunctionality() const { return is_func_family_; }; - + + bool GetFuncFromValid() const { + assert(valid_fam_size_ > -1); // CountFamSizeFromValid must be done first. + return is_func_from_valid_family_members; + }; + + int GetFamSize() { + if (fam_size_ < 0) { CountFamSizeFromAll(); } + return fam_size_; + }; + + int GetValidFamSize() { + if (valid_fam_size_ < 0) { CountFamSizeFromValid(); } + return valid_fam_size_; + }; + + void ResetValidFamilyMembers() { + valid_family_members.resize(0); + valid_family_members.reserve(all_family_members.size()); + valid_fam_size_ = -1; + is_func_from_valid_family_members = false; + }; + +protected: + bool is_func_from_family_members = false; + bool is_func_from_valid_family_members = false; + int fam_size_ = -1; // Total read counts in all_family_members (a consensus read counted by its read_count) + int valid_fam_size_ = -1; // Total read counts in valid_family_members (a consensus read counted by its read_count) +}; + + +class MolecularFamily : public AbstractMolecularFamily +{ +public: + MolecularFamily(const string& barcode = "", int strand = -1) + : AbstractMolecularFamily(barcode, strand) {}; + int CountFamSizeFromAll(); + int CountFamSizeFromValid(); + void SortAllFamilyMembers(); + void SortValidFamilyMembers(); + void ResetValidFamilyMembers(); + virtual ~MolecularFamily(){}; + bool is_all_family_members_sorted = false; + bool is_valid_family_members_sorted = false; +}; + + +// An interface of handling the tag structures. +class MolecularTagManager +{ private: - bool is_func_family_; + vector multisample_prefix_tag_struct_; + vector multisample_suffix_tag_struct_; + +public: + MolecularTagManager(); + MolecularTagTrimmer* tag_trimmer; + void Initialize(MolecularTagTrimmer* const input_tag_trimmer, const SampleManager* const sample_manager); + bool IsStrictTag(const string& prefix_tag, const string& suffix_tag, int sample_idx) const; + bool IsStrictPrefixTag(const string& prefix_tag, int sample_idx) const; + bool IsStrictSuffixTag(const string& suffix_tag, int sample_idx) const; + string GetPrefixTagStruct(int sample_indx) const {return multisample_prefix_tag_struct_[max(0, sample_indx)];}; // sample_idx = -1 indicates no multisample + string GetSuffixTagStruct(int sample_indx) const {return multisample_suffix_tag_struct_[max(0, sample_indx)];}; // sample_idx = -1 indicates no multisample + bool IsPartialSimilarTags(string tag_1, string tag_2, bool is_prefix) const; + bool IsFlowSynchronizedTags(string tag_1, string tag_2, bool is_prefix) const; }; -void GenerateMyMolecularFamilies(PositionInProgress &bam_position, - vector< vector< MolecularFamily > > &my_molecular_families, - const ExtendParameters ¶meters, - int sample_index = -1); +class MolecularFamilyGenerator +{ +private: + bool long_long_hashable_ = false; + const bool is_split_families_by_region_ = true; // I will always split families by region. + vector< map > long_long_tag_lookup_table_; + vector< map > string_tag_lookup_table_; + void SplitFamiliesByRegion_(vector< vector >& my_molecular_families) const; + void FindFamilyForOneRead_(Alignment* rai, vector< vector >& my_molecular_families); + long long BaseSeqToLongLong_(const string& base_seq) const; + char NucTo0123_(char nuc) const; +public: + MolecularFamilyGenerator() {}; + void GenerateMyMolecularFamilies(const MolecularTagManager* const mol_tag_manager, + PositionInProgress& bam_position, + int sample_index, + vector< vector >& my_molecular_families); +}; + + +void GenerateConsensusPositionTicket(vector< vector< vector > > &my_molecular_families_multisample, + VariantCallerContext &vc, + Consensus &consensus, + list::iterator &consensus_position_ticket, + bool filter_all_reads_after_done = false); -void RemoveNonFuncFamilies(vector< MolecularFamily > &my_molecular_families, unsigned int min_fam_size); +void GenerateCandidatesFromConsensusPositionTicket(AlleleParser* candidate_generator, + const BAMWalkerEngine* bam_walker, + deque& variant_candidates, + list::iterator& consensus_position_ticket, + int haplotype_length); #endif /* MOLECULARTAG_H */ diff --git a/Analysis/VariantCaller/OrderedVCFWriter.h b/Analysis/VariantCaller/OrderedVCFWriter.h index ba74f333..318163fa 100644 --- a/Analysis/VariantCaller/OrderedVCFWriter.h +++ b/Analysis/VariantCaller/OrderedVCFWriter.h @@ -35,7 +35,7 @@ class OrderedVCFWriter { } - void Initialize(const string& output_vcf, const ExtendParameters& parameters, ReferenceReader& ref_reader, const SampleManager& sample_manager) { + void Initialize(const string& output_vcf, const ExtendParameters& parameters, ReferenceReader& ref_reader, const SampleManager& sample_manager, bool use_molecular_tag = false) { string filtered_vcf; size_t pos = output_vcf.rfind("."); @@ -57,7 +57,7 @@ class OrderedVCFWriter { } suppress_no_calls_ = parameters.my_controls.suppress_no_calls; - string vcf_header = getVCFHeader(¶meters, ref_reader, sample_manager.sample_names_, sample_manager.primary_sample_); + string vcf_header = getVCFHeader(¶meters, ref_reader, sample_manager.sample_names_, sample_manager.primary_sample_, use_molecular_tag); output_vcf_stream_ << vcf_header << endl; filtered_vcf_stream_ << vcf_header << endl; variant_initializer_.parseHeader(vcf_header); diff --git a/Analysis/VariantCaller/Reads/ExtendedReadInfo.cpp b/Analysis/VariantCaller/Reads/ExtendedReadInfo.cpp index 372f7410..767dbb5c 100644 --- a/Analysis/VariantCaller/Reads/ExtendedReadInfo.cpp +++ b/Analysis/VariantCaller/Reads/ExtendedReadInfo.cpp @@ -27,7 +27,8 @@ void CreateFlowIndex(Alignment *rai, const ion::FlowOrder & flow_order) base_idx++; } if (base_idx != rai->read_bases.length()) { - cerr << "WARNING in ExtendedReadInfo::CreateFlowIndex: There are more bases in the read than fit into the flow order."; + cerr << "ERROR: in ExtendedReadInfo::CreateFlowIndex: There are more bases in the read than fit into the flow order.\t" << rai->alignment.Name << endl; + cerr << rai->read_bases << endl; exit(1); } } @@ -107,6 +108,7 @@ void UnpackAlignmentInfo(Alignment *rai) // ------------------------------------------------------- // Unpacking read meta data and filtering read if this is not possible +// This function is called after the candidate generator's UnpackReadAlleles void UnpackOnLoad(Alignment *rai, const InputStructures &global_context) { @@ -116,6 +118,7 @@ void UnpackOnLoad(Alignment *rai, const InputStructures &global_context) rai->is_reverse_strand = rai->alignment.IsReverseStrand(); + if (not rai->alignment.GetTag("ZR", rai->read_count)) {rai->read_count = 1;} // Parse read name, run id & flow order index rai->runid.clear(); @@ -156,9 +159,28 @@ void UnpackOnLoad(Alignment *rai, const InputStructures &global_context) } rai->measurements.assign(global_context.num_flows_by_run_id.at(rai->runid), 0.0); for (size_t counter = 0; counter < quantized_measurements.size(); ++counter) - rai->measurements[counter] = (float)quantized_measurements[counter]/256; + rai->measurements[counter] = (float) quantized_measurements[counter] / 256.0f; rai->measurements_length = quantized_measurements.size(); + // Retrieve measurements standard deviation from ZS tag + quantized_measurements.resize(0); // I reuse quantized_measurements for getting the ZS tag. + // measurements_sd appears only if it is a consensus read + if (rai->read_count > 1){ + if (rai->alignment.GetTag("ZS", quantized_measurements)) { + if ((int) quantized_measurements.size() != rai->measurements_length){ + cerr << "ERROR: Normalized measurements ZM:tag length " << rai->measurements_length + << " != measurements standard deviation ZS:tag length " << quantized_measurements.size() + <<" in read " << rai->alignment.Name << endl; + exit(1); + } + rai->measurements_sd.assign(quantized_measurements.size(), 0.0f); + for (size_t counter = 0; counter < quantized_measurements.size(); ++counter) + rai->measurements_sd[counter] = (float) quantized_measurements[counter] / 256.0f; + } + + } + + // Retrieve phasing parameters from ZP tag if (not rai->alignment.GetTag("ZP", rai->phase_params)) { @@ -200,12 +222,12 @@ void UnpackOnLoad(Alignment *rai, const InputStructures &global_context) rai->start_flow = 0; if (not rai->alignment.GetTag("ZF", rai->start_flow)) { - uint8_t start_flow_byte = 0; - if (not rai->alignment.GetTag("ZF", start_flow_byte)) { + uint32_t start_flow = 0; + if (not rai->alignment.GetTag("ZF", start_flow)) { cerr << "ERROR: Start Flow ZF:tag not found in read " << rai->alignment.Name << endl; exit(1); } - rai->start_flow = (int)start_flow_byte; + rai->start_flow = (int) start_flow; } if (rai->start_flow == 0) { cerr << "WARNING: Start Flow ZF:tag has zero value in read " << rai->alignment.Name << endl; @@ -263,7 +285,239 @@ void UnpackOnLoad(Alignment *rai, const InputStructures &global_context) } +// Unpack the information used to generate flow space consensus +void UnpackOnLoadLight(Alignment *rai, const InputStructures &global_context) +{ + // No need to waste time if the read is filtered + if (rai->filtered) + return; + + if (not rai->alignment.GetTag("ZR", rai->read_count)) {rai->read_count = 1;} + + // I really need the ZA tag! + + rai->is_reverse_strand = rai->alignment.IsReverseStrand(); + + // Parse read name, run id & flow order index + + rai->runid.clear(); + if (not rai->alignment.Name.empty()) { + rai->well_rowcol.resize(2); + ion_readname_to_rowcol(rai->alignment.Name.c_str(), &rai->well_rowcol[0], &rai->well_rowcol[1]); + // extract runid while we are at it + rai->runid = rai->alignment.Name.substr(0,rai->alignment.Name.find(":")); + } + + if (rai->runid.empty()){ + cerr << "WARNING: Unable to determine run id of read " << rai->alignment.Name << endl; + rai->filtered = true; + return; + } + + map::const_iterator fo_it = global_context.flow_order_index_by_run_id.find(rai->runid); + if (fo_it == global_context.flow_order_index_by_run_id.end()){ + cerr << "WARNING: No matching flow oder found for read " << rai->alignment.Name << endl; + rai->filtered = true; + return; + } + + rai->flow_order_index = fo_it->second; + const ion::FlowOrder & flow_order = global_context.flow_order_vector.at(rai->flow_order_index); + + // Retrieve measurements from ZM tag + // No need to do it here to reduce memory usage + /* + vector quantized_measurements; + if (not rai->alignment.GetTag("ZM", quantized_measurements)) { + cerr << "ERROR: Normalized measurements ZM:tag is not present in read " << rai->alignment.Name << endl; + exit(1); + } + if ((int)quantized_measurements.size() > global_context.num_flows_by_run_id.at(rai->runid)) { + cerr << "ERROR: Normalized measurements ZM:tag length " << quantized_measurements.size() + << " exceeds flow order length " << global_context.num_flows_by_run_id.at(rai->runid) + <<" in read " << rai->alignment.Name << endl; + exit(1); + } + + rai->measurements.assign(global_context.num_flows_by_run_id.at(rai->runid), 0.0); + for (size_t counter = 0; counter < quantized_measurements.size(); ++counter) + rai->measurements[counter] = (float) quantized_measurements[counter] / 256.0f; + rai->measurements_length = quantized_measurements.size(); + */ + if (not rai->alignment.HasTag("ZM")) { + cerr << "ERROR: Normalized measurements ZM:tag is not present in read " << rai->alignment.Name << endl; + exit(1); + } + + + // Retrieve phasing parameters from ZP tag + + if (not rai->alignment.GetTag("ZP", rai->phase_params)) { + cerr << "ERROR: Phasing Parameters ZP:tag is not present in read " << rai->alignment.Name << endl; + exit(1); + } + if (rai->phase_params.size() != 3) { + cerr << "ERROR: Phasing Parameters ZP:tag does not have 3 phase parameters in read " << rai->alignment.Name << endl; + exit(1); + } + if (rai->phase_params[0] < 0 or rai->phase_params[0] > 1 or rai->phase_params[1] < 0 or rai->phase_params[1] > 1 + or rai->phase_params[2] < 0 or rai->phase_params[2] > 1) { + cerr << "ERROR: Phasing Parameters ZP:tag outside of [0,1] range in read " << rai->alignment.Name << endl; + exit(1); + } + + //rai->phase_params[2] = 0.0f; // ad-hoc corrector: zero droop + + // Populate read_bases (bases without rev-comp on reverse-mapped reads) and flow_index + + // No need to do it here to reduce memory usage + /* + rai->read_bases = rai->alignment.QueryBases; + if (rai->is_reverse_strand) + RevComplementInPlace(rai->read_bases); + if (rai->read_bases.empty()){ + cerr << "WARNING: Ignoring length zero read " << rai->alignment.Name << endl; + rai->filtered = true; + return; + } + */ + if (rai->alignment.QueryBases.empty()){ + cerr << "WARNING: Ignoring length zero read " << rai->alignment.Name << endl; + rai->filtered = true; + return; + } + + rai->start_flow = 0; + if (not rai->alignment.GetTag("ZF", rai->start_flow)) { + uint32_t start_flow = 0; + if (not rai->alignment.GetTag("ZF", start_flow)) { + cerr << "ERROR: Start Flow ZF:tag not found in read " << rai->alignment.Name << endl; + exit(1); + } + rai->start_flow = (int) start_flow; + } + if (rai->start_flow == 0) { + cerr << "WARNING: Start Flow ZF:tag has zero value in read " << rai->alignment.Name << endl; + rai->filtered = true; + return; + } + + + // Check validity of input arguments + if (rai->start_flow < 0 or rai->start_flow >= global_context.num_flows_by_run_id.at(rai->runid)) { + cerr << "ERROR: Start flow outside of [0,num_flows) range in read " << rai->alignment.Name << endl; + cerr << "Start flow: " << rai->start_flow << " Number of flows: " << global_context.flow_order_vector.at(rai->flow_order_index).num_flows(); + exit(1); + } + + // Retrieve read group name & generate prefix flow + + if (not rai->alignment.GetTag("RG",rai->read_group)) { + cerr << "WARNING: No read group found in read " << rai->alignment.Name << endl; + // No big problem, we'll just have to solve the prefix like it's 2013! + rai->read_group.clear(); + } + + // Get read prefix - hard clipped start of the read: [KS][ZT][ZE] + rai->prefix_flow = -1; + map::const_iterator key_it = global_context.key_by_read_group.find(rai->read_group); + if (key_it != global_context.key_by_read_group.end()) { + rai->prefix_bases = key_it->second; + + string temp_zt, temp_ze; + if (rai->alignment.GetTag("ZT", temp_zt)) + rai->prefix_bases += temp_zt; + if (rai->alignment.GetTag("ZE", temp_ze)) + rai->prefix_bases += temp_ze; + + if (not rai->prefix_bases.empty()) + GetPrefixFlow(rai, rai->prefix_bases, flow_order); + } + + // Get read suffix + rai->suffix_bases.clear(); + string temp_yt, temp_ye; + if (rai->alignment.GetTag("YE", temp_ye)) + rai->suffix_bases += temp_ye; + if (rai->alignment.GetTag("YT", temp_yt)) + rai->suffix_bases += temp_yt; + + if (not rai->prefix_bases.empty()) + GetPrefixFlow(rai, rai->prefix_bases, flow_order); + + + // Check consistency of prefix_flow and start_flow - maybe we don't have all info about hard clipped bases + char read_bases_at_0 = rai->is_reverse_strand? NucComplement(rai->alignment.QueryBases.back()) : rai->alignment.QueryBases[0]; + if (rai->prefix_flow >= 0) { + int check_start_flow = rai->prefix_flow; + while (check_start_flow < flow_order.num_flows() and flow_order.nuc_at(check_start_flow) != read_bases_at_0) + check_start_flow++; + if (check_start_flow != rai->start_flow) { + rai->prefix_flow = -1; + rai->prefix_bases.clear(); + cerr << "WARNING: Missing hard-clipped prefix bases in read "<< rai->alignment.Name << endl; + rai->filtered = true; + cerr << "start_flow = "<< rai->start_flow<<", check_start_flow = " << check_start_flow<::iterator target_idx_it = rai->target_coverage_indices.begin(); target_idx_it != rai->target_coverage_indices.end(); ++target_idx_it){ + int read_mismatch_limit_by_target = targets_manager->unmerged[*target_idx_it].read_mismatch_limit; + // See if override by this target? (-1 means no override) + if (read_mismatch_limit_by_target >= 0){ + if (is_override_by_target){ + // The read covers multiple targets with override. + if (read_mismatch_limit == 0 or read_mismatch_limit_by_target == 0){ + read_mismatch_limit = max(read_mismatch_limit, read_mismatch_limit_by_target); + }else{ + read_mismatch_limit = min(read_mismatch_limit, read_mismatch_limit_by_target); + } + }else{ + // This is the first time I see the override. + is_override_by_target = true; + read_mismatch_limit = read_mismatch_limit_by_target; + } + } + } + } + + if (rai->filtered or read_mismatch_limit == 0){ + return; + } + + int modified_read_mismatches = 0; + if (not rai->alignment.GetTag("NM", modified_read_mismatches)){ + cerr << "WARNING: Filter out the read "<< rai->alignment.Name << " w/o NM tag. Set read-mismatch-limit=0 to disable the check." << endl; + rai->filtered = true; + return; + } + // Note that the NM tag is number of all mismatched "bases", e.g. one gap open of X bases counts X. + for (vector::iterator cigar_it = rai->alignment.CigarData.begin(); cigar_it != rai->alignment.CigarData.end(); ++cigar_it){ + if ((cigar_it->Type == 'I' or cigar_it->Type == 'D') and cigar_it->Length > 0){ + modified_read_mismatches -= (int) (cigar_it->Length - 1); // One gap open or one insertion counts one in modified mismatches. + } + } + if (modified_read_mismatches < 0){ + // Something very wrong that I can catch. + cerr << "WARNING: The read "<< rai->alignment.Name << " is filtered out because of inconsistent CIGAR and NM tags. Set read-mismatch-limit=0 to disable the check." << endl; + rai->filtered = true; + } + if (modified_read_mismatches > read_mismatch_limit){ + rai->filtered = true; + } +} diff --git a/Analysis/VariantCaller/Reads/ExtendedReadInfo.h b/Analysis/VariantCaller/Reads/ExtendedReadInfo.h index d435d1e7..6dc9e06a 100644 --- a/Analysis/VariantCaller/Reads/ExtendedReadInfo.h +++ b/Analysis/VariantCaller/Reads/ExtendedReadInfo.h @@ -19,10 +19,7 @@ using namespace std; struct Alignment; void UnpackOnLoad(Alignment *rai, const InputStructures &global_context); - -//! @brief Creates a stack of reads that provide evidence in the case of our candidate variant -void StackUpOneVariant(vector& read_stack, int variant_start_pos, int variant_end_pos, - const ExtendParameters ¶meters, const PositionInProgress& bam_position); - +void UnpackOnLoadLight(Alignment *rai, const InputStructures &global_context); +void FilterByModifiedMismatches(Alignment *rai, int read_mismatch_limit, const TargetsManager *const targets_manager); #endif //EXTENDEDREADINFO_H diff --git a/Analysis/VariantCaller/SampleManager.cpp b/Analysis/VariantCaller/SampleManager.cpp index 6f976177..74b14ffe 100644 --- a/Analysis/VariantCaller/SampleManager.cpp +++ b/Analysis/VariantCaller/SampleManager.cpp @@ -12,26 +12,31 @@ // const string& force_sample_name, // const string& sample_name, +// ----------------------------------------------------------------------------------------- +// Function extracts the samples associated with read groups in bam_header +// primary_sample_name [in]: sets a primary sample name (default is first found in header) +// only the primary sample will be analyzed if we don't specify multi-sample analysis +// force_sample_name [in]: ignores all information in the BAM and force analyzes as one sample +// multisample [in/out]: activates multi-sample analysis for more than one sample in BAM -void SampleManager::Initialize (const SamHeader& bam_header, string& sample_name, const string& force_sample_name) + +void SampleManager::Initialize (const SamHeader& bam_header, const string& primary_sample_name, const string& force_sample_name, bool &multisample) { + // Iterate through samples in BAM header to extract sample information num_samples_ = 0; - + primary_sample_name_ = primary_sample_name; + for (SamReadGroupConstIterator read_group = bam_header.ReadGroups.Begin(); read_group < bam_header.ReadGroups.End(); ++read_group) { - string barcode = read_group->PlatformUnit; - string::size_type pos = barcode.rfind("/"); - if (pos != string::npos) {barcode = barcode.substr(pos + 1);} + string sample_name; if (force_sample_name.empty()) { - //sample_name = read_group->Sample + "." + barcode; sample_name = read_group->Sample; } else { sample_name = force_sample_name; } - if (read_group->ID.empty()) { cerr << "ERROR: One of BAM read groups is missing ID tag" << endl; exit(1); @@ -65,46 +70,57 @@ void SampleManager::Initialize (const SamHeader& bam_header, string& sample_name // if it's the same sample name and RG combo, no worries // TODO: what about other tags } + //cout << "SampleManager: Read group " << read_group->ID << " is associated with sample " << sample_name << endl; + } if (num_samples_ == 0) { cerr << "ERROR: BAM file(s) do not have any read group definitions" << endl; exit(1); } + // Do we have a multi-sample analysis? + else if (num_samples_ > 1) { + if (not multisample and primary_sample_name_.empty()){ + cerr << "ERROR: SampleManager: Multiple Samples (" << num_samples_ << ") found in BAM file/s provided. "<< endl; + //cerr << "ERROR: But neither a primary sample was provided nor multi-sample analysis enabled." << endl; + cerr << "ERROR: Please select primary sample name to process using the \"--sample-name\" parameter. " << endl; + //cerr << "ERROR: AND/OR enable multi-sample analysis using the \"--multisample-analysis\" parameter. " << endl; + exit(EXIT_FAILURE); + } + } + else // We only have one sample + multisample = false; + // Search for specified primary sample or select a default primary (first available) bool default_sample = false; - //now check if there are multiple samples in the BAM file and if so user should provide a sampleName to process - if (num_samples_ == 1 && sample_name.empty()) { - sample_name = sample_names_[0]; + if (primary_sample_name_.empty()) { + primary_sample_name_ = sample_names_[0]; default_sample = true; - - } else if (num_samples_ > 1 && sample_name.empty()) { - sample_name = sample_names_[0]; - //cerr << "ERROR: Multiple Samples found in BAM file/s provided. Torrent Variant Caller currently supports variant calling on only one sample. " << endl; - //cerr << "ERROR: Please select sample name to process using -g parameter. " << endl; - //exit(1); } bool primary_sample_found = false; for (int i = 0; i < num_samples_; ++i) { - if (sample_names_[i] == sample_name) { + if (sample_names_[i] == primary_sample_name_) { primary_sample_ = i; primary_sample_found = true; } + // AWalt added this because of IR-19679? + // TODO investigate why this is actually necessary? else { - string test = sample_name + "."; + string test = primary_sample_name_ + "."; if (strncmp(sample_names_[i].c_str(), test.c_str(), test.length()) == 0) { primary_sample_ = i; primary_sample_found = true; + primary_sample_name_ = sample_names_[primary_sample_]; } } } if (!primary_sample_found) { - cerr << "ERROR: Sample " << sample_name << " provided using -g option " + cerr << "ERROR: Sample " << primary_sample_name << " provided using \"--sample-name\" option " << "is not associated with any read groups in BAM file(s)" << endl; - exit(1); + exit(EXIT_FAILURE); } //now find the read group ID associated with this sample name @@ -113,20 +129,22 @@ void SampleManager::Initialize (const SamHeader& bam_header, string& sample_name if (primary_sample_ == p->second) num_primary_read_groups++; + if (multisample) + cout << "SampleManager: Multi-sample analysis enabled." << endl; if (!force_sample_name.empty()) cout << "SampleManager: All read groups forced to assume sample name " << force_sample_name << endl; cout << "SampleManager: Found " << read_group_to_sample_idx_.size() << " read group(s) and " << num_samples_ << " sample(s)." << endl; if (default_sample) - cout << "SampleManager: Primary sample \"" << sample_name << "\" (default) present in " << num_primary_read_groups << " read group(s)" << endl; + cout << "SampleManager: Primary sample \"" << primary_sample_name_ << "\" (default) present in " << num_primary_read_groups << " read group(s)" << endl; else - cout << "SampleManager: Primary sample \"" << sample_name << "\" (set via -g) " << num_primary_read_groups << " read group(s)" << endl; + cout << "SampleManager: Primary sample \"" << primary_sample_name_ << "\" (set via -g) " << num_primary_read_groups << " read group(s)" << endl; } +// -------------------------------------------------------------------- +// This function populates sample_index and primary_sample flag for read alignment objects - -//bool SampleManager::IdentifySample(Alignment& ra) const bool SampleManager::IdentifySample(const BamAlignment& alignment, int& sample_index, bool& primary_sample) const { diff --git a/Analysis/VariantCaller/SampleManager.h b/Analysis/VariantCaller/SampleManager.h index d431a6a0..cefc13e0 100644 --- a/Analysis/VariantCaller/SampleManager.h +++ b/Analysis/VariantCaller/SampleManager.h @@ -23,13 +23,14 @@ class SampleManager { SampleManager() : num_samples_(0), primary_sample_(0) {} ~SampleManager() {} - void Initialize (const SamHeader& bam_header, string& sample_name, const string& force_sample_name); + void Initialize (const SamHeader& bam_header, const string& primary_sample_name, const string& force_sample_name, bool &multisample); bool IdentifySample(const BamAlignment& alignment, int& sample_index, bool& primary_sample) const; - int num_samples_; - vector sample_names_; - map read_group_to_sample_idx_; - int primary_sample_; + int num_samples_; // Number of samples found in BAM(s) + vector sample_names_; // Names of samples + map read_group_to_sample_idx_; // Map of read group names to sample index + int primary_sample_; // index of the primary sample + string primary_sample_name_; // short for sample_names_[primary_sample_] }; diff --git a/Analysis/VariantCaller/Splice/ClassifyVariant.cpp b/Analysis/VariantCaller/Splice/ClassifyVariant.cpp index ecd55771..dce2af46 100644 --- a/Analysis/VariantCaller/Splice/ClassifyVariant.cpp +++ b/Analysis/VariantCaller/Splice/ClassifyVariant.cpp @@ -6,7 +6,7 @@ #include "ClassifyVariant.h" #include "ErrorMotifs.h" -#include "StackEngine.h" +#include "CrossHypotheses.h" // This function only works for the 1Base -> 1 Base snp representation void AlleleIdentity::SubCategorizeSNP(const LocalReferenceContext &reference_context) { @@ -202,35 +202,26 @@ void AlleleIdentity::SubCategorizeMNP(const LocalReferenceContext &reference_con // Test whether this is an HP-InDel void AlleleIdentity::IdentifyHPdeletion(const LocalReferenceContext& reference_context) { - // Get right anchor for better HP-InDel classification - right_anchor = 0; - // It's a deletion, so reference allele must be longer than alternative allele - int shorter_test_pos = altAllele.length() - 1; - int longer_test_pos = reference_context.reference_allele.length() - 1; - while (shorter_test_pos >= left_anchor and - altAllele[shorter_test_pos] == reference_context.reference_allele[longer_test_pos]) { - right_anchor++; - shorter_test_pos--; - longer_test_pos--; - } - - if (left_anchor+right_anchor < (int)altAllele.length()){ + if (left_anchor+right_anchor != (int) altAllele.length()){ // If the anchors do not add up to the length of the shorter allele, // a more complex substitution happened and we don't classify as HP-InDel status.isHPIndel = false; } else { - status.isHPIndel = reference_context.my_hp_length[left_anchor] > 1; - for (int i_base=left_anchor+1; (status.isHPIndel and i_base<(int)reference_context.reference_allele.length()-right_anchor); i_base++){ - status.isHPIndel = status.isHPIndel and (reference_context.my_hp_length[left_anchor] > 1); - } - } - inDelLength = reference_context.reference_allele.length() - altAllele.length(); + //status.isHPIndel = (left_anchor+right_anchor) == (int) altAllele.length(); + //for (int i_base=left_anchor+1; (status.isHPIndel and i_base<(int)reference_context.reference_allele.length()-right_anchor); i_base++){ + // status.isHPIndel = status.isHPIndel and (reference_context.my_hp_length[left_anchor] > 1); + //} + string padding_ref = string(1, reference_context.ref_left_hp_base) + reference_context.reference_allele + string(1, reference_context.ref_right_hp_base); + string padding_alt = string(1, reference_context.ref_left_hp_base) + altAllele + string(1, reference_context.ref_right_hp_base); + status.isHPIndel = IsHpIndel(padding_ref, padding_alt); + } + inDelLength = abs((int) reference_context.reference_allele.length() - (int) altAllele.length()); } // Test whether this is an HP-InDel void AlleleIdentity::IdentifyHPinsertion(const LocalReferenceContext& reference_context, - const ReferenceReader &ref_reader, int chr_idx) { + const ReferenceReader &ref_reader) { char ref_base_right_of_anchor; int ref_right_hp_length = 0; @@ -254,7 +245,7 @@ void AlleleIdentity::IdentifyHPinsertion(const LocalReferenceContext& reference_ status.isHPIndel = true; ref_hp_length = ref_right_hp_length; } - inDelLength = altAllele.length() - reference_context.reference_allele.length(); + inDelLength = abs((int) altAllele.length() - (int) reference_context.reference_allele.length()); if (status.isHPIndel) { for (int b_idx = left_anchor + 1; b_idx < left_anchor + inDelLength; b_idx++) { @@ -263,7 +254,7 @@ void AlleleIdentity::IdentifyHPinsertion(const LocalReferenceContext& reference_ } } else if (inDelLength == 1) { status.isHPIndel = IdentifyDyslexicMotive(altAllele[left_anchor], reference_context.position0+left_anchor, - ref_reader, chr_idx); + ref_reader, reference_context.chr_idx); } } @@ -318,7 +309,7 @@ bool AlleleIdentity::IdentifyDyslexicMotive(char base, int position, // We categorize InDels bool AlleleIdentity::SubCategorizeInDel(const LocalReferenceContext& reference_context, - const ReferenceReader &ref_reader, int chr_idx) { + const ReferenceReader &ref_reader) { // These fields are set no matter what status.isDeletion = (reference_context.reference_allele.length() > altAllele.length()); @@ -329,7 +320,7 @@ bool AlleleIdentity::SubCategorizeInDel(const LocalReferenceContext& reference_c ref_hp_length = reference_context.my_hp_length[left_anchor]; } else { // Insertion - IdentifyHPinsertion(reference_context, ref_reader, chr_idx); + IdentifyHPinsertion(reference_context, ref_reader); } if (DEBUG > 0){ @@ -345,7 +336,7 @@ bool AlleleIdentity::SubCategorizeInDel(const LocalReferenceContext& reference_c bool AlleleIdentity::CharacterizeVariantStatus(const LocalReferenceContext &reference_context, - const ReferenceReader &ref_reader, int chr_idx) + const ReferenceReader &ref_reader) { //cout << "Hello from CharacterizeVariantStatus; " << altAllele << endl; bool is_ok = true; @@ -356,37 +347,58 @@ bool AlleleIdentity::CharacterizeVariantStatus(const LocalReferenceContext &refe status.isPaddedSNP = false; status.doRealignment = false; - // Get Anchor length + if (altAllele == reference_context.reference_allele){ + status.isNoVariant = true; + status.isProblematicAllele = true; + status.isSNP = (altAllele.size() == 1); + status.isMNV = not (status.isSNP); + is_ok = false; + // I don't want to continue since it is not a variant. + return is_ok; + } + + // Get left anchor length ref_hp_length = reference_context.my_hp_length[0]; left_anchor = 0; - unsigned int a_idx = 0; - while (a_idx < altAllele.length() and a_idx < reference_context.reference_allele.length() - and altAllele[a_idx] == reference_context.reference_allele[a_idx]) { - a_idx++; - left_anchor++; + while (left_anchor < (int) altAllele.length() and left_anchor < ref_length + and altAllele[left_anchor] == reference_context.reference_allele[left_anchor]) { + ++left_anchor; + } + // Get right anchor length + // right anchor is obtained after I remove left anchor, which implies I prefer left alignment. + right_anchor = 0; + // It's a deletion, so reference allele must be longer than alternative allele + int alt_test_pos = (int) altAllele.length() - 1; + int ref_test_pos = ref_length - 1; + while (alt_test_pos >= left_anchor + and ref_test_pos >= left_anchor + and altAllele[alt_test_pos] == reference_context.reference_allele[ref_test_pos]) { + right_anchor++; + alt_test_pos--; + ref_test_pos--; + } + // Calculate the variant window (in reference coordinate) + start_variant_window = position0 + num_padding_added.first; + end_variant_window = position0 + ref_length - num_padding_added.second; + if (num_padding_added.first > 0 or num_padding_added.second > 0){ + assert(reference_context.reference_allele.substr(0, num_padding_added.first) == altAllele.substr(0, num_padding_added.first)); + assert(reference_context.reference_allele.substr((int) reference_context.reference_allele.size() - num_padding_added.second) == altAllele.substr((int) altAllele.size() - num_padding_added.second)); + assert(start_variant_window < end_variant_window); } - if (DEBUG > 0) - cout << "- Alternative Allele " << altAllele << " (anchor length " << left_anchor << ") "; + if (DEBUG > 0) + cout << "- Alternative Allele " << altAllele << " (left anchor length = " << left_anchor << " , right anchor length = " << right_anchor << ")"; - const string& ref_allele = reference_context.reference_allele; - const string& alt_allele = altAllele; - int ref_length = ref_allele.length(); - int alt_length = alt_allele.length(); - while (alt_length > 1 and ref_length > 1 and alt_allele[alt_length-1] == ref_allele[ref_length-1]) { - --alt_length; - --ref_length; - } - int prefix = 0; - while (prefix < alt_length and prefix < ref_length and alt_allele[prefix] == ref_allele[prefix]) - ++prefix; - ref_length -= prefix; - alt_length -= prefix; + // ref_length is the length of the anchor-removed reference allele + int ref_length = (int) reference_context.reference_allele.length() - (left_anchor + right_anchor); + // alt_length is the length of the anchor-removed alt allele + int alt_length = (int) altAllele.length() - (left_anchor + right_anchor); + assert(ref_length >= 0 and alt_length >= 0); // Change classification to better reflect what we can get with haplotyping if (altAllele.length() != reference_context.reference_allele.length()) { status.isIndel = true; - is_ok = SubCategorizeInDel(reference_context, ref_reader, chr_idx); + is_ok = SubCategorizeInDel(reference_context, ref_reader); } else if ((int)altAllele.length() == 1) { // Categorize function only works with this setting status.isSNP = true; @@ -396,8 +408,7 @@ bool AlleleIdentity::CharacterizeVariantStatus(const LocalReferenceContext &refe } else { status.isMNV = true; ref_hp_length = reference_context.my_hp_length[left_anchor]; - if (ref_length == 1 and alt_length == 1) - status.isPaddedSNP = true; + status.isPaddedSNP = (ref_length == 1 and alt_length == 1); SubCategorizeMNP(reference_context); if (DEBUG > 0) cout << " is an MNP." << endl; @@ -434,28 +445,34 @@ bool AlleleIdentity::getVariantType( const TIonMotifSet & ErrorMotifs, const ClassifyFilters &filter_variant, const ReferenceReader &ref_reader, - int chr_idx) { + const pair &alt_orig_padding) { altAllele = _altAllele; + position0 = (int) reference_context.position0; + ref_length = (int) reference_context.reference_allele.length(); + chr_idx = reference_context.chr_idx; + num_padding_added = alt_orig_padding; + bool is_ok = reference_context.context_detected; + // check position does not beyond the chromosome + is_ok *= not ((reference_context.position0 + (long) altAllele.length()) > ref_reader.chr_size(reference_context.chr_idx)); - if ((reference_context.position0 + (long)altAllele.length()) > ref_reader.chr_size(chr_idx)) { - is_ok = false; - } + // check alternative allele contains TACG only + is_ok *= CheckValidAltAllele(reference_context); // We should now be guaranteed a valid variant position in here if (is_ok) { - is_ok = CharacterizeVariantStatus(reference_context, ref_reader, chr_idx); - PredictSequenceMotifSSE(reference_context, ErrorMotifs, ref_reader, chr_idx); + is_ok = CharacterizeVariantStatus(reference_context, ref_reader); } - is_ok = is_ok and CheckValidAltAllele(reference_context); - if (!is_ok) { + if (is_ok) { + PredictSequenceMotifSSE(reference_context, ErrorMotifs, ref_reader); + }else{ status.isProblematicAllele = true; filterReasons.push_back("BADCANDIDATE"); } - return(is_ok); + return is_ok; } @@ -474,54 +491,45 @@ void AlleleIdentity::ModifyStartPosForAllele(int variantPos) { // Logic: When shifting a window of the same period as the MNR, the base entering the window has to be equal to the base leaving the window. // example with period 2: XYZACACA|CA|CACAIJK bool AlleleIdentity::IdentifyMultiNucRepeatSection(const LocalReferenceContext &seq_context, unsigned int rep_period, - const ReferenceReader &ref_reader, int chr_idx) { + const ReferenceReader &ref_reader) { //cout << "Hello from IdentifyMultiNucRepeatSection with period " << rep_period << "!"<< endl; unsigned int variantPos = seq_context.position0 + left_anchor; - if (variantPos + rep_period >= (unsigned long)ref_reader.chr_size(chr_idx)) - return (false); - - CircluarBuffer window(rep_period); - for (unsigned int idx = 0; idx < rep_period; idx++) - window.assign(idx, ref_reader.base(chr_idx,variantPos+idx)); + if (variantPos + rep_period >= (unsigned long)ref_reader.chr_size(seq_context.chr_idx)) + return false; - // Investigate (inclusive) start position of MNR region - start_window = variantPos - 1; // 1 anchor base - window.shiftLeft(1); - while (start_window > 0 and window.first() == ref_reader.base(chr_idx,start_window)) { - start_window--; - window.shiftLeft(1); - } + CircluarBuffer window(0); + start_splicing_window = seq_context.FindSplicingStartForMNR(ref_reader, variantPos, rep_period, window); // Investigate (exclusive) end position of MNR region - end_window = variantPos + rep_period; - if (end_window >= ref_reader.chr_size(chr_idx)) + end_splicing_window = variantPos + rep_period; + if (end_splicing_window >= ref_reader.chr_size(seq_context.chr_idx)) return false; for (unsigned int idx = 0; idx < rep_period; idx++) - window.assign(idx, ref_reader.base(chr_idx,variantPos+idx)); + window.assign(idx, ref_reader.base(seq_context.chr_idx,variantPos+idx)); window.shiftRight(1); - while (end_window < ref_reader.chr_size(chr_idx) and window.last() == ref_reader.base(chr_idx,end_window)) { - end_window++; + while (end_splicing_window < ref_reader.chr_size(seq_context.chr_idx) and window.last() == ref_reader.base(seq_context.chr_idx,end_splicing_window)) { + end_splicing_window++; window.shiftRight(1); } //cout << "Found repeat stretch of length: " << (end_window - start_window) << endl; // Require that a stretch of at least 3*rep_period has to be found to count as a MNR - if ((end_window - start_window) >= (3*(int)rep_period)) { + if ((end_splicing_window - start_splicing_window) >= (3*(int)rep_period)) { // Correct start and end of the window if they are not fully outside variant allele - if (start_window >= seq_context.position0) - start_window = seq_context.my_hp_start_pos[0] - 1; - if (end_window <= seq_context.right_hp_start) { + if (start_splicing_window >= seq_context.position0) + start_splicing_window = seq_context.StartSplicingExpandFromMyHpStart0(); + if (end_splicing_window <= seq_context.right_hp_start) { if (status.isInsertion) - end_window = seq_context.right_hp_start + seq_context.right_hp_length + 1; + end_splicing_window = seq_context.right_hp_start + seq_context.right_hp_length + 1; else - end_window = seq_context.right_hp_start + 1; + end_splicing_window = seq_context.right_hp_start + 1; } - if (start_window < 0) - start_window = 0; - if (end_window > ref_reader.chr_size(chr_idx)) - end_window = ref_reader.chr_size(chr_idx); + if (start_splicing_window < 0) + start_splicing_window = 0; + if (end_splicing_window > ref_reader.chr_size(seq_context.chr_idx)) + end_splicing_window = ref_reader.chr_size(seq_context.chr_idx); return (true); } else @@ -532,26 +540,27 @@ bool AlleleIdentity::IdentifyMultiNucRepeatSection(const LocalReferenceContext & // ----------------------------------------------------------------- -void AlleleIdentity::CalculateWindowForVariant(const LocalReferenceContext &seq_context, int DEBUG, - const ReferenceReader &ref_reader, int chr_idx) { +void AlleleIdentity::CalculateWindowForVariant(const LocalReferenceContext &seq_context, + const ReferenceReader &ref_reader) { // If we have an invalid vcf candidate, set a length zero window and exit if (!seq_context.context_detected or status.isProblematicAllele) { - start_window = seq_context.position0; - end_window = seq_context.position0; + cout <<" I am probematic!" < 0) { cout << "MNR found in allele " << seq_context.reference_allele << " -> " << altAllele << endl; - cout << "Window for allele " << altAllele << ": (" << start_window << ") "; - for (int p_idx = start_window; p_idx < end_window; p_idx++) - cout << ref_reader.base(chr_idx,p_idx); - cout << " (" << end_window << ") " << endl; + cout << "Window for allele " << altAllele << ": (" << start_splicing_window << ") "; + for (int p_idx = start_splicing_window; p_idx < end_splicing_window; p_idx++) + cout << ref_reader.base(seq_context.chr_idx,p_idx); + cout << " (" << end_splicing_window << ") " << endl; } return; // Found a matching period and computed window } @@ -559,44 +568,47 @@ void AlleleIdentity::CalculateWindowForVariant(const LocalReferenceContext &seq_ // not an MNR. Moving on along to InDels. if (status.isIndel) { // Default variant window - end_window = seq_context.right_hp_start +1; // Anchor base to the right of allele - start_window = seq_context.position0; + end_splicing_window = seq_context.right_hp_start +1; // Anchor base to the right of allele + start_splicing_window = seq_context.StartSplicingNoExpansion(); // Adjustments if necessary if (status.isDeletion) if (seq_context.my_hp_start_pos[left_anchor] == seq_context.my_hp_start_pos[0]) - start_window = seq_context.my_hp_start_pos[0] - 1; + start_splicing_window = seq_context.StartSplicingExpandFromMyHpStart0(); if (status.isInsertion) { if (left_anchor == 0) { - start_window = seq_context.my_hp_start_pos[0] - 1; + start_splicing_window = seq_context.StartSplicingExpandFromMyHpStart0(); } else if (altAllele[left_anchor] == altAllele[left_anchor - 1] and seq_context.position0 > (seq_context.my_hp_start_pos[left_anchor - 1] - 1)) { - start_window = seq_context.my_hp_start_pos[left_anchor - 1] - 1; + start_splicing_window = seq_context.StartSplicingExpandFromMyHpStartLeftAnchor(left_anchor); } if (altAllele[altAllele.length() - 1] == seq_context.ref_right_hp_base) { - end_window += seq_context.right_hp_length; + end_splicing_window += seq_context.right_hp_length; } } // Safety - if (start_window < 0) - start_window = 0; - if (end_window > ref_reader.chr_size(chr_idx)) - end_window = ref_reader.chr_size(chr_idx); + if (start_splicing_window < 0) + start_splicing_window = 0; + if (end_splicing_window > ref_reader.chr_size(seq_context.chr_idx)) + end_splicing_window = ref_reader.chr_size(seq_context.chr_idx); } else { // SNPs and MNVs are 1->1 base replacements - start_window = seq_context.position0; - end_window = seq_context.position0 + seq_context.reference_allele.length(); - } // */ + start_splicing_window = seq_context.StartSplicingNoExpansion(); + end_splicing_window = seq_context.position0 + seq_context.reference_allele.length(); + } + + // Final safety: splicing window is a super set of the interval spanned by the reference allele. + assert(start_splicing_window <= (int) seq_context.position0 and end_splicing_window >= (int) seq_context.position0 + (int) seq_context.reference_allele.length()); if (DEBUG > 0) { - cout << "Window for allele " << altAllele << ": (" << start_window << ") "; - for (int p_idx = start_window; p_idx < end_window; p_idx++) - cout << ref_reader.base(chr_idx,p_idx); - cout << " (" << end_window << ") " << endl; + cout << "Window for allele " << altAllele << ": (" << start_splicing_window << ") "; + for (int p_idx = start_splicing_window; p_idx < end_splicing_window; p_idx++) + cout << ref_reader.base(seq_context.chr_idx,p_idx); + cout << " (" << end_splicing_window << ") " << endl; } } @@ -606,7 +618,7 @@ void AlleleIdentity::CalculateWindowForVariant(const LocalReferenceContext &seq_ void AlleleIdentity::PredictSequenceMotifSSE(const LocalReferenceContext &reference_context, const TIonMotifSet & ErrorMotifs, - const ReferenceReader &ref_reader, int chr_idx) { + const ReferenceReader &ref_reader) { //cout << "Hello from PredictSequenceMotifSSE" << endl; sse_prob_positive_strand = 0; @@ -622,7 +634,7 @@ void AlleleIdentity::PredictSequenceMotifSSE(const LocalReferenceContext &refere unsigned context_left = var_position >= 10 ? 10 : var_position; //if (var_position + reference_context.my_hp_length.at(left_anchor) + 10 < ref_reader.chr_size(chr_idx)) - seqContext = ref_reader.substr(chr_idx, var_position - context_left, context_left + (unsigned int)reference_context.my_hp_length[left_anchor] + 10); + seqContext = ref_reader.substr(reference_context.chr_idx, var_position - context_left, context_left + (unsigned int)reference_context.my_hp_length[left_anchor] + 10); // else // seqContext = ref_reader.substr(chr_idx, var_position - context_left); @@ -651,104 +663,163 @@ void AlleleIdentity::DetectLongHPThresholdCases(const LocalReferenceContext &seq } } +void AlleleIdentity::DetectHpIndelCases(const vector &hp_indel_hrun, const vector &hp_ins_len, const vector &hp_del_len) { + if (status.isIndel && status.isHPIndel and inDelLength > 0) { + assert((hp_indel_hrun.size() == hp_ins_len.size()) and (hp_indel_hrun.size() == hp_del_len.size())); + for (unsigned int i_hp = 0; i_hp < hp_indel_hrun.size(); ++i_hp) { + if (ref_hp_length == hp_indel_hrun[i_hp]){ + if (status.isInsertion and inDelLength <= hp_ins_len[i_hp]){ + filterReasons.push_back("HPINSLEN"); + status.isProblematicAllele = true; + } + else if (status.isDeletion and inDelLength <= hp_del_len[i_hp]){ + filterReasons.push_back("HPDELLEN"); + status.isProblematicAllele = true; + } + } + } + } +} + + void AlleleIdentity::DetectNotAVariant(const LocalReferenceContext &seq_context) { - if (altAllele.compare(seq_context.reference_allele) == 0) { + if (status.isNoVariant) { //incorrect allele status is passed thru make it a no call status.isProblematicAllele = true; filterReasons.push_back("NOTAVARIANT"); } } - -void AlleleIdentity::DetectCasesToForceNoCall(const LocalReferenceContext &seq_context, const ClassifyFilters &filter_variant, +void AlleleIdentity::DetectCasesToForceNoCall(const LocalReferenceContext &seq_context, const ControlCallAndFilters& my_controls, const VariantSpecificParams& variant_specific_params) { DetectNotAVariant(seq_context); DetectLongHPThresholdCases(seq_context, variant_specific_params.hp_max_length_override ? - variant_specific_params.hp_max_length : filter_variant.hp_max_length); + variant_specific_params.hp_max_length : my_controls.filter_variant.hp_max_length); + DetectHpIndelCases(my_controls.filter_variant.filter_hp_indel_hrun, my_controls.filter_variant.filter_hp_ins_len, my_controls.filter_variant.filter_hp_del_len); } -// ==================================================================== - - -void EnsembleEval::SetupAllAlleles(const ExtendParameters ¶meters, - const InputStructures &global_context, - const ReferenceReader &ref_reader, - int chr_idx) -{ - seq_context.DetectContext(*variant, global_context.DEBUG, ref_reader, chr_idx); - allele_identity_vector.resize(variant->alt.size()); - - if (global_context.DEBUG > 0 and variant->alt.size()>0) { - cout << "Investigating variant candidate " << seq_context.reference_allele - << " -> " << variant->alt[0]; - for (uint8_t i_allele = 1; i_allele < allele_identity_vector.size(); i_allele++) - cout << ',' << variant->alt[i_allele]; - cout << endl; - } - - //now calculate the allele type (SNP/Indel/MNV/HPIndel etc.) and window for hypothesis calculation for each alt allele. - for (uint8_t i_allele = 0; i_allele < allele_identity_vector.size(); i_allele++) { - - // TODO: Hotspot should be an allele property but we only set all or none to Hotspots, depending on the vcf record - allele_identity_vector[i_allele].status.isHotSpot = variant->isHotSpot; - allele_identity_vector[i_allele].filterReasons.clear(); - allele_identity_vector[i_allele].DEBUG = global_context.DEBUG; - - allele_identity_vector[i_allele].indelActAsHPIndel = parameters.my_controls.filter_variant.indel_as_hpindel; - - allele_identity_vector[i_allele].getVariantType(variant->alt[i_allele], seq_context, - global_context.ErrorMotifs, parameters.my_controls.filter_variant, ref_reader, chr_idx); - allele_identity_vector[i_allele].CalculateWindowForVariant(seq_context, global_context.DEBUG, ref_reader, chr_idx); - } - - //GetMultiAlleleVariantWindow(); - multiallele_window_start = -1; - multiallele_window_end = -1; +// ==================================================================== - // Mark Ensemble for realignment if any of the possible variants should be realigned - // TODO: Should we exclude already filtered alleles? - for (uint8_t i_allele = 0; i_allele < allele_identity_vector.size(); i_allele++) { - //if (!allele_identity_vector[i_allele].status.isNoCallVariant) { - if (allele_identity_vector[i_allele].start_window < multiallele_window_start or multiallele_window_start == -1) - multiallele_window_start = allele_identity_vector[i_allele].start_window; - if (allele_identity_vector[i_allele].end_window > multiallele_window_end or multiallele_window_end == -1) - multiallele_window_end = allele_identity_vector[i_allele].end_window; - if (allele_identity_vector[i_allele].ActAsSNP() && parameters.my_controls.filter_variant.do_snp_realignment) { - doRealignment = doRealignment or allele_identity_vector[i_allele].status.doRealignment; - } - if (allele_identity_vector[i_allele].ActAsMNP() && parameters.my_controls.filter_variant.do_mnp_realignment) { - doRealignment = doRealignment or allele_identity_vector[i_allele].status.doRealignment; - } - } - // Hack: pass allele windows back down the object - for (uint8_t i_allele = 0; i_allele < allele_identity_vector.size(); i_allele++) { - allele_identity_vector[i_allele].start_window = multiallele_window_start; - allele_identity_vector[i_allele].end_window = multiallele_window_end; - } - if (global_context.DEBUG > 0) { - cout << "Realignment for this candidate is turned " << (doRealignment ? "on" : "off") << endl; - cout << "Final window for multi-allele: " << ": (" << multiallele_window_start << ") "; - for (int p_idx = multiallele_window_start; p_idx < multiallele_window_end; p_idx++) - cout << ref_reader.base(chr_idx,p_idx); - cout << " (" << multiallele_window_end << ") " << endl; - } +// win_1 = [win1_start, win1_end), win_2 = [win2_start, win2_end), +// return (win_1 \cap win_2 != \emptyset) where \cap is the set intersection operator. +template +bool IsOverlappingWindows(MyIndexType win1_start, MyIndexType win1_end, MyIndexType win2_start, MyIndexType win2_end) +{ + if (win1_start >= win1_end or win2_start >= win2_end){ + // win_1 or win_2 is an empty set => empty set intersects any set is always empty + return false; + } + return (win1_start < win2_end) and (win1_end > win2_start); } -// ------------------------------------------------------------ - -void EnsembleEval::FilterAllAlleles(const ClassifyFilters &filter_variant, const vector& variant_specific_params) { - if (seq_context.context_detected) { - for (uint8_t i_allele = 0; i_allele < allele_identity_vector.size(); i_allele++) { - allele_identity_vector[i_allele].DetectCasesToForceNoCall(seq_context, filter_variant, variant_specific_params[i_allele]); - } - } +// Is there splicing hazard of this allele interfered by alt_x? +// Splicing hazard happened if the (my splicing window) \cap (my variant window)^c overlaps (alt_x's variant window) +// where \cap means intersection, ^c means complement. +bool AlleleIdentity::DetectSplicingHazard(const AlleleIdentity& alt_x) const{ + bool is_splicing_hazard = IsOverlappingWindows(start_splicing_window, start_variant_window, alt_x.start_variant_window, alt_x.end_variant_window) + or IsOverlappingWindows(end_variant_window, end_splicing_window, alt_x.start_variant_window, alt_x.end_variant_window); + return is_splicing_hazard; } - - +// The two alleles are connected (i.e., need to be evaluated together) if any of the following conditions is satisfied +// a) (variant window of alt1) intersects (variant window of alt2) +// b) There is splicing hazard of alt1 interfered by alt2. +// c) There is splicing hazard of alt2 interfered by alt1. +// Note: Fake HS allele means the HS allele has no read support. It won't interfere other alleles. But I need to make sure other alleles don't interfere it. +bool IsAllelePairConnected(const AlleleIdentity& alt1, const AlleleIdentity& alt2) +{ + bool debug = alt1.DEBUG or alt2.DEBUG; + // Alleles start at the same position should be evaluated together. + bool is_connect = alt1.start_variant_window == alt2.start_variant_window; + + // Print the allele information for debug + if (debug){ + cout << "+ Detecting connectivity of the allele pair (altX, altY) = (" + << alt1.altAllele << "@[" << alt1.position0 << ", " << alt1.position0 + alt1.ref_length << "), " + << alt2.altAllele << "@[" << alt2.position0 << ", " << alt2.position0 + alt2.ref_length << "))" << endl; + cout << " - (altX, altY) is Fake HS Allele? (" << alt1.status.isFakeHsAllele << ", " << alt2.status.isFakeHsAllele <<")" << endl; + } + + // Rule number one: start at the same position must be connected. + if (is_connect){ + if (debug){ + cout << " - Connected: altX and altY start at the same position." << endl; + } + return is_connect; + } + + // Exception for problematic alleles + if (alt1.status.isProblematicAllele or alt2.status.isProblematicAllele){ + if (debug){ + cout << (is_connect? " - Connected: altX or altY is problematic at the same position." : " - Not connected: altX or altY is problematic.") << endl; + } + return is_connect; + } + + // Exceptions for Fake HS alleles + if (alt1.status.isFakeHsAllele and alt2.status.isFakeHsAllele){ + if (debug){ + cout << (is_connect? " - Connected: both fake HS at the same position." : " - Not connected: both fake HS." ) << endl; + } + return is_connect; + }else if (alt1.status.isFakeHsAllele and alt1.ref_length >= 10 and (not alt1.status.doRealignment) + and alt2.status.isHPIndel and alt2.inDelLength == 1){ + if (debug){ + cout << (is_connect? " - Connected: altX and altY start at the same position." : " - Not connected: long Fake HS altX meets 1-mer HP-INDEL altY." ) << endl; + } + return is_connect; + }else if (alt2.status.isFakeHsAllele and alt2.ref_length >= 10 and (not alt2.status.doRealignment) + and alt1.status.isHPIndel and alt1.inDelLength == 1){ + if (debug){ + cout << (is_connect? " - Connected: altX and altY start at the same position." : " - Not connected: 1-mer HP-INDEL altX meets long Fake HS altY." ) << endl; + } + return is_connect; + } + + // Condition a) + bool is_variant_window_overlap = IsOverlappingWindows(alt1.start_variant_window, alt1.end_variant_window, alt2.start_variant_window, alt2.end_variant_window); + // Condition b) + bool is_alt1_interfered_by_alt2 = false; + if (not alt2.status.isFakeHsAllele){ + is_alt1_interfered_by_alt2 = alt1.DetectSplicingHazard(alt2); + } + // Condition c) + bool is_alt2_interfered_by_alt1 = false; + if (not alt1.status.isFakeHsAllele){ + is_alt2_interfered_by_alt1 = alt2.DetectSplicingHazard(alt1); + } + is_connect = is_variant_window_overlap or is_alt1_interfered_by_alt2 or is_alt2_interfered_by_alt1; + + // Print debug message + if (debug){ + if (not is_connect){ + cout << " - Not connected." << endl; + } + else{ + if (is_variant_window_overlap){ + cout << " - Connected: Overlapping variant windows: " + << "var_win_X = [" << alt1.start_variant_window << ", "<< alt1.end_variant_window << "), " + << "var_win_Y = [" << alt2.start_variant_window << ", "<< alt2.end_variant_window << ")" << endl; + } + if (is_alt1_interfered_by_alt2){ + cout << " - Connected: Splicing hazard of altX interfered by altY: " + << "splice_win_X = [" << alt1.start_splicing_window << ", "<< alt1.end_splicing_window << "), " + << "var_win_X = [" << alt1.start_variant_window << ", "<< alt1.end_variant_window << "), " + << "var_win_Y = [" << alt2.start_variant_window << ", "<< alt2.end_variant_window << ")" << endl; + } + if (is_alt2_interfered_by_alt1){ + cout << " - Connected: Splicing hazard of altY interfered by altX: " + << "splice_win_Y = [" << alt2.start_splicing_window << ", "<< alt2.end_splicing_window << "), " + << "var_win_Y = [" << alt2.start_variant_window << ", "<< alt2.end_variant_window << "), " + << "var_win_X = [" << alt1.start_variant_window << ", "<< alt1.end_variant_window << ")" << endl; + } + } + } + return is_connect; +} diff --git a/Analysis/VariantCaller/Splice/ClassifyVariant.h b/Analysis/VariantCaller/Splice/ClassifyVariant.h index 8672815c..b6b00ed8 100644 --- a/Analysis/VariantCaller/Splice/ClassifyVariant.h +++ b/Analysis/VariantCaller/Splice/ClassifyVariant.h @@ -53,9 +53,11 @@ class VarButton { //bool isComplex; // A complex allele is anything but snp, mnv and Indel //bool isComplexHP; // This complex allele involves a ref. HP of length > 1 - + bool isHotSpotAllele; // Signifies the hotspot allele + bool isFakeHsAllele; // Signifies a "fake" hotspot variant, i.e., the candidate generator sees no read supports me. bool isHotSpot; // Signifies a hotspot variant (set per variant for all alleles, regardless of their specific origin) bool isProblematicAllele; // There is something wrong with this allele, we should filter it. + bool isNoVariant; // The alternative allele is not a variant, i.e., altAllele = reference_context.reference_allele bool doRealignment; // Switch to turn realignment on or off VarButton() { @@ -68,78 +70,34 @@ class VarButton { isPaddedSNP = false; isIndel = false; isHotSpot = false; + isHotSpotAllele= false; + isFakeHsAllele = false; isProblematicAllele = false; + isNoVariant = false; doRealignment = false; } }; -// ------------------------------------------------------------------------ - -// example variants: -// VarButton isSNP true -// genome 44 45 46 47 48 49 (0 based) -// ref is A A A A T T -// alt is A -// altAllele is A -// left_anchor 0 (always) -// right_anchor 0 (always) -// inDelLength 0 (always) -// ref_hp_length 2 (>=1) -- T T satrting at 48 -// start_window <=48 -- calculated as min over all alt Alleles -// end_window >=49 -- calculated as max over all alt Alleles - -// VarButton isMNV true -// genome 44 45 46 47 48 49 (0 based) -// ref is A A A A T T -// alt is A G C -// altAllele is A G C -// left_anchor 1 -// right_anchor 0 -// inDelLength 0 (always) -// ref_hp_length 2 (>=1 always) -- T T starting at 48 -// start_window -// end_window - -// VarButton isIndel true, isDeletion false -// genome 42 42 44 45 46 47 48 49 50 51 52 (0 based) -// ref is C A A A A T G T A A A -// alt is d C G -// altAllele is T C G -// left_anchor 1 -// right_anchor 0 -// inDelLength 2 -// ref_hp_length 1 (G at 49) -// start_window -// end_window - - -// VarButton isIndel false, isInsertion true -// genome 42 42 44 45 46 47 48 49 50 51 52 (0 based) -// ref is C C A A A A T G T A A A -// alt is G GC -// altAllele is G G C -// left_anchor 0 -// right_anchor 0 -// inDelLength 3 -// ref_hp_length 4 (A at 47) -// start_window -// end_window - - class AlleleIdentity { public: VarButton status; //!< A bunch of flags saying what's going on with this allele string altAllele; //!< May contain left and/or right anchor bases, cannot be empty int DEBUG; - + int chr_idx; //!< Chromosome index> + int position0; //! + int ref_length; //! // useful context int left_anchor; //!< Number of left bases that are common between the ref. and alt. allele int right_anchor; //!< Number of right bases that are common between the ref. and alt. allele // left_anchor + right_anchor <= shorter allele length int inDelLength; //!< Difference in length between longer and shorter allele int ref_hp_length; //!< First base change is occurring in an HP of length ref_hp_length - int start_window; //!< Start of window of interest for this variant - int end_window; //!< End of window of interest for this variant + int start_splicing_window; //!< Start of splicing window of interest for this alt allele. + int end_splicing_window; //!< End of splicing window of interest for this alt allele => splicing window = [start_splicing_window, end_splicing_window) + int multiallele_window_start; //!< Start of splicing window of interest for the multi-allele variant + int multiallele_window_end; //!< End of splicing window of interest for multi-allele variant + int start_variant_window; //!< Start of the anchor-removed window of interest for this alt allele + int end_variant_window; //!< End of the anchor-removed window of interest for this alt allele => variant window = [start_variant_window, end_variant_window) // need to know when I do filtering float sse_prob_positive_strand; @@ -148,15 +106,28 @@ class AlleleIdentity { bool indelActAsHPIndel; // Switch to make all indels match HPIndel behavior - AlleleIdentity() { + // Level of flow-disruption vs ref: (-1, 0, 1, 2) = (indefinite, HP-INDEL, otherwise , FD) + int fd_level_vs_ref; + + // Extra (prefix, suffix) padding bases added into the alt allele if the alt allele representation is obtained by grouping with other alt alleles. + // (Note): For an standard indel representation e.g., T->TT, num_padding_added = (0, 0). + pair num_padding_added; + AlleleIdentity() { + position0 = -1; + chr_idx = -1; + ref_length = -1; inDelLength = 0; ref_hp_length = 0; //modified_start_pos = 0; left_anchor = 0; right_anchor = 0; - start_window = 0; - end_window = 0; + start_splicing_window = 0; + end_splicing_window = 0; + multiallele_window_start = 0; + multiallele_window_end = 0; + start_variant_window = 0; + end_variant_window = 0; DEBUG = 0; // filterable statuses @@ -164,34 +135,34 @@ class AlleleIdentity { sse_prob_negative_strand = 0; indelActAsHPIndel = false; + fd_level_vs_ref = -1; }; - bool Ordinary() { - return(status.isIndel && !(status.isHPIndel)); + bool Ordinary() const { + return (status.isIndel and (not status.isHPIndel)); }; - bool ActAsSNP(){ - // return(status.isSNP || status.isMNV || (status.isIndel && !status.isHPIndel)); + bool ActAsSNP() const { if (indelActAsHPIndel) - return(status.isSNP || status.isPaddedSNP); + return (status.isSNP or status.isPaddedSNP); else - return(status.isSNP || status.isPaddedSNP || (status.isIndel && !status.isHPIndel)); + return (status.isSNP or status.isPaddedSNP or (status.isIndel and (not status.isHPIndel))); } - bool ActAsMNP(){ - return(status.isMNV); + bool ActAsMNP() const { + return status.isMNV; } - bool ActAsHPIndel(){ + bool ActAsHPIndel() const { if (indelActAsHPIndel) - return(status.isIndel); + return status.isIndel; else - return(status.isIndel && status.isHPIndel); + return (status.isIndel and status.isHPIndel); } //void DetectPotentialCorrelation(const LocalReferenceContext &reference_context); bool SubCategorizeInDel(const LocalReferenceContext &reference_context, - const ReferenceReader &ref_reader, int chr_idx); + const ReferenceReader &ref_reader); void IdentifyHPdeletion(const LocalReferenceContext& reference_context); void IdentifyHPinsertion(const LocalReferenceContext& reference_context, - const ReferenceReader &ref_reader, int chr_idx); + const ReferenceReader &ref_reader); bool IdentifyDyslexicMotive(char base, int position, const ReferenceReader &ref_reader, int chr_idx); @@ -201,26 +172,31 @@ class AlleleIdentity { const TIonMotifSet & ErrorMotifs, const ClassifyFilters &filter_variant, const ReferenceReader &ref_reader, - int chr_idx); + const pair &alt_orig_padding); bool CharacterizeVariantStatus(const LocalReferenceContext &reference_context, - const ReferenceReader &ref_reader, int chr_idx); + const ReferenceReader &ref_reader); bool CheckValidAltAllele(const LocalReferenceContext &reference_context); //void ModifyStartPosForAllele(int variantPos); bool IdentifyMultiNucRepeatSection(const LocalReferenceContext &seq_context, unsigned int rep_period, - const ReferenceReader &ref_reader, int chr_idx); - void CalculateWindowForVariant(const LocalReferenceContext &seq_context, int DEBUG, - const ReferenceReader &ref_reader, int chr_idx); + const ReferenceReader &ref_reader); + + void CalculateWindowForVariant(const LocalReferenceContext &seq_context, + const ReferenceReader &ref_reader); - void DetectCasesToForceNoCall(const LocalReferenceContext &seq_context, const ClassifyFilters &filter_variant, + void DetectCasesToForceNoCall(const LocalReferenceContext &seq_context, const ControlCallAndFilters& my_controls, const VariantSpecificParams& variant_specific_params); void DetectLongHPThresholdCases(const LocalReferenceContext &seq_context, int maxHPLength); + void DetectHpIndelCases(const vector &hp_indel_hrun, const vector &hp_ins_len, const vector &hp_del_len); void DetectNotAVariant(const LocalReferenceContext &seq_context); void PredictSequenceMotifSSE(const LocalReferenceContext &reference_context, const TIonMotifSet & ErrorMotifs, - const ReferenceReader &ref_reader, int chr_idx); + const ReferenceReader &ref_reader); + bool DetectSplicingHazard(const AlleleIdentity& alt_x) const; }; +bool IsAllelePairConnected(const AlleleIdentity& alt1, const AlleleIdentity& alt2); - +template +bool IsOverlappingWindows(MyIndexType win1_start, MyIndexType win1_end, MyIndexType win2_start, MyIndexType win2_end); #endif //CLASSIFYVARIANT_H diff --git a/Analysis/VariantCaller/Splice/LocalContext.cpp b/Analysis/VariantCaller/Splice/LocalContext.cpp index 528a20e4..7b22c3a1 100644 --- a/Analysis/VariantCaller/Splice/LocalContext.cpp +++ b/Analysis/VariantCaller/Splice/LocalContext.cpp @@ -6,18 +6,30 @@ #include "LocalContext.h" - +void LocalReferenceContext::DetectContextAtPosition(const ReferenceReader &ref_reader, int my_chr_idx, int position0, int ref_len){ + assert(ref_len > 0); + chr_idx = my_chr_idx; + vcf::Variant dummy_variant; + dummy_variant.sequenceName = ref_reader.chr_str(chr_idx); + dummy_variant.position = (long) (position0 + 1); + dummy_variant.ref = ref_reader.substr(chr_idx, (long) position0, (long) ref_len); + DetectContext(dummy_variant, 0, ref_reader); +} void LocalReferenceContext::DetectContext(const vcf::Variant &candidate_variant, int DEBUG, - const ReferenceReader &ref_reader, int chr_idx) { + const ReferenceReader &ref_reader) { // VCF stores positions in 1-based index; local_contig_sequence has a zero based index // all positions in this object are zero based so that they correspond to reference in memory. position0 = candidate_variant.position-1; contigName = candidate_variant.sequenceName; + if (chr_idx < 0) + chr_idx = ref_reader.chr_idx(contigName.c_str()); + else if (contigName != ref_reader.chr_str(chr_idx)) + chr_idx = ref_reader.chr_idx(contigName.c_str()); // Sanity checks if position is valid and reference allele matches reference - if (!ContextSanityChecks(candidate_variant, ref_reader, chr_idx)) + if (!ContextSanityChecks(candidate_variant, ref_reader)) return; my_hp_length.resize(reference_allele.length(), 0); @@ -105,14 +117,12 @@ void LocalReferenceContext::DetectContext(const vcf::Variant &candidate_variant, } bool LocalReferenceContext::ContextSanityChecks(const vcf::Variant &candidate_variant, - const ReferenceReader &ref_reader, int chr_idx) { + const ReferenceReader &ref_reader) { // Sanity checks that abort context detection reference_allele = candidate_variant.ref; context_detected = true; - - if (candidate_variant.position < 1 or candidate_variant.position > (long)ref_reader.chr_size(chr_idx)) { cerr << "Non-fatal ERROR: Candidate Variant Position is not within the Contig Bounds at VCF Position " << candidate_variant.sequenceName << ":" << candidate_variant.position @@ -163,3 +173,54 @@ bool LocalReferenceContext::ContextSanityChecks(const vcf::Variant &candidate_va return (context_detected); } + +// return the minimum possible splicing window start in this context +int LocalReferenceContext::SplicingLeftBound(const ReferenceReader &ref_reader) const{ + if (not context_detected){ + return StartSplicingNoExpansion(); + } + int splicing_left_bound = StartSplicingNoExpansion(); // splicing window start for SNP/MNP + splicing_left_bound = min(splicing_left_bound, StartSplicingExpandFromMyHpStart0()); // splicing window start for INDEL + for (int rep_period = min_mnr_rep_period; rep_period <= max_mnr_rep_period; ++rep_period){ + CircluarBuffer mnr_window(0); + int mnr_start = FindSplicingStartForMNR(ref_reader, position0, rep_period, mnr_window); + splicing_left_bound = min(splicing_left_bound, mnr_start); // splicing window start for MNR + } + splicing_left_bound = max(0, splicing_left_bound); // safety + return splicing_left_bound; +} + +// Splicing start for MNR only depends on context, so I do it here. +int LocalReferenceContext::FindSplicingStartForMNR(const ReferenceReader &ref_reader, int variant_pos, int rep_period, CircluarBuffer& window) const{ + int mnr_start = variant_pos; + if (variant_pos + rep_period >= (int) ref_reader.chr_size(chr_idx)){ + return mnr_start; + } + + --mnr_start; // 1 anchor base + window = CircluarBuffer(rep_period); + for (int idx = 0; idx < rep_period; idx++){ + window.assign(idx, ref_reader.base(chr_idx, variant_pos + idx)); + } + + // Investigate (inclusive) start position of MNR region + window.shiftLeft(1); + while (mnr_start > 0 and window.first() == ref_reader.base(chr_idx, mnr_start)) { + mnr_start--; + window.shiftLeft(1); + } + return mnr_start; +} + +// Originally, the start splicing window was calculated in AlleleIdentity::CalculateWindowForVariant. +// Now LocalReferenceContext::SplicingLeftBound wants to calculate the left bound of the splicing window, +// so I calculate the splicing window start here to make sure that LocalReferenceContext can handle all possible start splicing windows. +int LocalReferenceContext::StartSplicingNoExpansion() const{ + return (int) position0; +} +int LocalReferenceContext::StartSplicingExpandFromMyHpStart0() const{ + return my_hp_start_pos[0] - 1; +} +int LocalReferenceContext::StartSplicingExpandFromMyHpStartLeftAnchor(int left_anchor) const{ + return my_hp_start_pos[left_anchor - 1] - 1; +} diff --git a/Analysis/VariantCaller/Splice/LocalContext.h b/Analysis/VariantCaller/Splice/LocalContext.h index e1f24d81..bc3578a8 100644 --- a/Analysis/VariantCaller/Splice/LocalContext.h +++ b/Analysis/VariantCaller/Splice/LocalContext.h @@ -19,6 +19,7 @@ #include #include "ReferenceReader.h" +#include "MiscUtil.h" using namespace std; @@ -44,8 +45,10 @@ class LocalReferenceContext{ // all positions in this object are zero based so that they correspond to reference in memory. long position0; //!< Zero based allele start position. string contigName; //!< Contig Name from VCF + int chr_idx; //!< index associated with contigName bool context_detected; //!< Check to see if the reference allele matches the given genome position. + // Information about the bases in the reference allele string reference_allele; //!< The reference allele for this variant vector my_hp_length; //!< Member HP length for each base in the reference allele. @@ -59,11 +62,15 @@ class LocalReferenceContext{ char ref_right_hp_base; //!< Base comprising the HP to the right of the one containing the vcf position int right_hp_length; //!< Length of the HP to the left of of the HP encompassing position int right_hp_start; + // MNR + int min_mnr_rep_period; + int max_mnr_rep_period; LocalReferenceContext(){ my_hp_length.clear(); my_hp_start_pos.clear(); context_detected = false; + chr_idx = -1; position0 = -1; ref_left_hp_base = 'X'; // Something that can't occur in the reference ref_right_hp_base = 'X'; @@ -71,17 +78,30 @@ class LocalReferenceContext{ left_hp_length = 0; right_hp_start = 0; right_hp_length = 0; + min_mnr_rep_period = 2; + max_mnr_rep_period = 5; } //! @brief Fills in the member variables void DetectContext(const vcf::Variant &candidate_variant, int DEBUG, - const ReferenceReader &ref_reader, int chr_idx); - + const ReferenceReader &ref_reader); + //! @brief Detect the context for the single base reference at position0 (zero-based) only. + void DetectContextAtPosition(const ReferenceReader &ref_reader, int my_chr_idx, int position0, int ref_len); //! @brief Basic sanity checks on the provided vcf positions //! Sets member context_detected to true if sanity checks are passed. //! Returns false if VCF position is not valid. bool ContextSanityChecks(const vcf::Variant &candidate_variant, - const ReferenceReader &ref_reader, int chr_idx); + const ReferenceReader &ref_reader); + //! @brief Calculate the left bound of the splicing window start in this context + int SplicingLeftBound(const ReferenceReader &ref_reader) const; + //! @brief Calculate the splicing window start for MNR (if any) in this context + int FindSplicingStartForMNR(const ReferenceReader &ref_reader, int variant_pos, int rep_period, CircluarBuffer& window) const; + //! @brief Calculate the splicing window start if I don't want to expand the splicing window to the left (primarily for SNP, MNP) + int StartSplicingNoExpansion() const; + //! @brief Calculate the splicing window start if I want to expand the splicing window to the left from the start position (primarily for INDEL) + int StartSplicingExpandFromMyHpStart0() const; + //! @brief Calculate the splicing window start if I want to expand the splicing window to the left from the anchor-removed start position (primarily for some INS) + int StartSplicingExpandFromMyHpStartLeftAnchor(int left_anchor) const; }; #endif //LOCALCONTEXT_H diff --git a/Analysis/VariantCaller/Splice/SpliceVariantHypotheses.cpp b/Analysis/VariantCaller/Splice/SpliceVariantHypotheses.cpp index 2248b922..d14fb193 100644 --- a/Analysis/VariantCaller/Splice/SpliceVariantHypotheses.cpp +++ b/Analysis/VariantCaller/Splice/SpliceVariantHypotheses.cpp @@ -9,7 +9,7 @@ bool SpliceVariantHypotheses(const Alignment ¤t_read, const EnsembleEval & const LocalReferenceContext &local_context, PersistingThreadObjects &thread_objects, int &splice_start_flow, int &splice_end_flow, vector &my_hypotheses, vector & same_as_null_hypothesis, bool & changed_alignment, const InputStructures &global_context, - const ReferenceReader &ref_reader, int chr_idx) + const ReferenceReader &ref_reader) { // Hypotheses: 1) Null; read as called 2) Reference Hypothesis 3-?) Variant Hypotheses @@ -51,7 +51,7 @@ bool SpliceVariantHypotheses(const Alignment ¤t_read, const EnsembleEval & // do realignment of a small region around variant if desired if (my_ensemble.doRealignment) { pretty_alignment = SpliceDoRealignement(thread_objects, current_read, local_context.position0, - changed_alignment, global_context.DEBUG, ref_reader, chr_idx); + changed_alignment, global_context.DEBUG, ref_reader, local_context.chr_idx); if (pretty_alignment.empty() and global_context.DEBUG > 0) cerr << "Realignment returned an empty string in read " << current_read.alignment.Name << endl; } @@ -70,8 +70,8 @@ bool SpliceVariantHypotheses(const Alignment ¤t_read, const EnsembleEval & // Basic sanity checks if (read_idx >= read_idx_max - or ref_idx > ref_reader.chr_size(chr_idx) - or (ref_idx == ref_reader.chr_size(chr_idx) and pretty_alignment[pretty_idx] != '+')) { + or ref_idx > ref_reader.chr_size(local_context.chr_idx) + or (ref_idx == ref_reader.chr_size(local_context.chr_idx) and pretty_alignment[pretty_idx] != '+')) { did_splicing = false; break; } @@ -93,7 +93,7 @@ bool SpliceVariantHypotheses(const Alignment ¤t_read, const EnsembleEval & // Have reference bases inside of window but outside of span of reference allele if (outside_ref_allele and !outside_of_window and pretty_alignment[pretty_idx] != '+') { for (unsigned int i_hyp = 1; i_hyp < my_hypotheses.size(); i_hyp++) - my_hypotheses[i_hyp].push_back(ref_reader.base(chr_idx,ref_idx)); + my_hypotheses[i_hyp].push_back(ref_reader.base(local_context.chr_idx,ref_idx)); } // Have read bases as called outside of variant window diff --git a/Analysis/VariantCaller/Splice/SpliceVariantHypotheses.h b/Analysis/VariantCaller/Splice/SpliceVariantHypotheses.h index 33ae36d1..a7140e72 100644 --- a/Analysis/VariantCaller/Splice/SpliceVariantHypotheses.h +++ b/Analysis/VariantCaller/Splice/SpliceVariantHypotheses.h @@ -25,7 +25,7 @@ bool SpliceVariantHypotheses(const Alignment ¤t_read, const EnsembleEval & const LocalReferenceContext &local_context, PersistingThreadObjects &thread_objects, int &splice_start_flow, int &splice_end_flow, vector &my_hypotheses, vector & same_as_null_hypothesis, bool & changed_alignment, const InputStructures &global_context, - const ReferenceReader &ref_reader, int chr_idx); + const ReferenceReader &ref_reader); bool SpliceAddVariantAlleles(const Alignment ¤t_read, const string& pretty_alignment, diff --git a/Analysis/VariantCaller/TargetsManager.cpp b/Analysis/VariantCaller/TargetsManager.cpp index 1c71fcee..734423cd 100644 --- a/Analysis/VariantCaller/TargetsManager.cpp +++ b/Analysis/VariantCaller/TargetsManager.cpp @@ -8,6 +8,7 @@ #include #include +#include #include "BAMWalkerEngine.h" //#include "ExtendParameters.h" @@ -15,10 +16,13 @@ TargetsManager::TargetsManager() { trim_ampliseq_primers = false; + min_coverage_fraction = 0.0f; + pthread_mutex_init(&coverage_counter_mutex_, NULL); } TargetsManager::~TargetsManager() { + pthread_mutex_destroy(&coverage_counter_mutex_); } bool CompareTargets(TargetsManager::UnmergedTarget *i, TargetsManager::UnmergedTarget *j) @@ -47,13 +51,12 @@ bool CompareTargets(TargetsManager::UnmergedTarget *i, TargetsManager::UnmergedT -void TargetsManager::Initialize(const ReferenceReader& ref_reader, const string& _targets, bool _trim_ampliseq_primers /*const ExtendParameters& parameters*/) +void TargetsManager::Initialize(const ReferenceReader& ref_reader, const string& _targets, float min_cov_frac, bool _trim_ampliseq_primers /*const ExtendParameters& parameters*/) { - + min_coverage_fraction = min_cov_frac; // // Step 1. Retrieve raw target definitions // - list raw_targets; if (not _targets.empty()) { @@ -137,35 +140,35 @@ void TargetsManager::Initialize(const ReferenceReader& ref_reader, const string& } - +// ------------------------------------------------------------------------------------- void TargetsManager::LoadRawTargets(const ReferenceReader& ref_reader, const string& bed_filename, list& raw_targets) { - FILE *bed_file = fopen(bed_filename.c_str(), "r"); - if (not bed_file) { + ifstream bedfile(bed_filename.c_str()); + if (not bedfile.is_open()){ cerr << "ERROR: Unable to open target file " << bed_filename << " : " << strerror(errno) << endl; exit(1); } - char line[4096]; - char chr_name[4096]; - int begin; - int end; - char region_name[4096]; + string line, bed_field; + vector bed_line; int line_number = 0; - while (fgets(line, 4096, bed_file)) { - ++line_number; + while(getline(bedfile, line)){ - if (strncmp(line,"track",5) == 0) { - // Parse track line if needed + ++line_number; + // Skip header line(s) + if (line.compare(0,5,"track")==0 or line.compare(0,7,"browser")==0 or line.length()==0) continue; - } + // Split line into tab separated fields + bed_line.clear(); + stringstream ss(line); + while (getline(ss, bed_field, '\t')) + bed_line.push_back(bed_field); - int num_fields = sscanf(line, "%s\t%d\t%d\t%s", chr_name, &begin, &end, region_name); - if (num_fields == 0) - continue; + // the first three columns are required in the bad format + unsigned int num_fields = bed_line.size(); if (num_fields < 3) { cerr << "ERROR: Failed to parse target file line " << line_number << endl; exit(1); @@ -173,91 +176,118 @@ void TargetsManager::LoadRawTargets(const ReferenceReader& ref_reader, const str raw_targets.push_back(UnmergedTarget()); UnmergedTarget& target = raw_targets.back(); - target.begin = begin; - target.end = end; - target.chr = ref_reader.chr_idx(chr_name); - if (num_fields > 3 and strcmp(region_name,".") != 0) - target.name = region_name; - - if (target.chr < 0) { - cerr << "ERROR: Target region " << target.name << " (" << chr_name << ":" << begin << "-" << end << ")" + target.chr = ref_reader.chr_idx(bed_line[0].c_str()); + target.begin = strtol (bed_line[1].c_str(), NULL, 0); + target.end = strtol (bed_line[2].c_str(), NULL, 0); + + if (num_fields > 3 and bed_line[3]!=".") + target.name = bed_line[3]; + + // Validate target + if (target.chr < 0){ + cerr << "ERROR: Target region " << target.name << " (" << bed_line[0] << ":" << bed_line[1] << "-" << bed_line[2] << ")" << " has unrecognized chromosome name" << endl; exit(1); } - - if (begin < 0 || end > ref_reader.chr_size(target.chr)) { - cerr << "ERROR: Target region " << target.name << " (" << chr_name << ":" << begin << "-" << end << ")" + if (target.begin < 0 || target.end > ref_reader.chr_size(target.chr)) { + cerr << "ERROR: Target region " << target.name << " (" << bed_line[0] << ":" << bed_line[1] << "-" << bed_line[2] << ")" << " is outside of reference sequence bounds (" - << chr_name << ":0-" << ref_reader.chr_size(target.chr) << ")" << endl; + << bed_line[0] << ":0-" << ref_reader.chr_size(target.chr) << ")" << endl; exit(1); } - if (end < begin) { - cerr << "ERROR: Target region " << target.name << " (" << chr_name << ":" << begin << "-" << end << ")" + if (target.end < target.begin) { + cerr << "ERROR: Target region " << target.name << " (" << bed_line[0] << ":" << bed_line[1] << "-" << bed_line[2] << ")" << " has inverted coordinates" << endl; exit(1); } - AddExtraTrim(target, line, num_fields); - } - fclose(bed_file); + // And now we simply assume that we have a beddetail file with at least 5 columns + if (num_fields > 4) + ParseBedInfoField(target, bed_line[num_fields-1]); + } + bedfile.close(); if (raw_targets.empty()) { - cerr << "ERROR: No targets loaded from " << bed_filename - << " after parsing " << line_number << " lines" << endl; + cerr << "ERROR: No targets loaded from " << bed_filename << " after parsing " << line_number << " lines" << endl; exit(1); } } -void TargetsManager::AddExtraTrim(UnmergedTarget& target, char *line, int num_fields) +// ------------------------------------------------------------------------------------- + +bool ParseInfoKey(const string key, string info, long &value) { + + size_t found = info.find(key); + if (found == string::npos){ + return false; + } + + info.erase(0, found+key.size()); + value = strtol (info.c_str(), NULL, 0); + if (value>=0) + return true; + else + return false; +} + +// ------------------------------------------------------------------------------------- + +void TargetsManager::ParseBedInfoField(UnmergedTarget& target, const string info) { - // parse extra trimming out of the bed file - // would be nice to unify with validate_bed using BedFile.h - char keybuffer[4096]; + // parse extra parameters out of the bed file info fields + long int temp = 0; + target.trim_left = 0; + if (ParseInfoKey("TRIM_LEFT=", info, temp)) + target.trim_left = temp; + target.trim_right = 0; + if (ParseInfoKey("TRIM_RIGHT=", info, temp)) + target.trim_right = temp; - int numfields = sscanf(line, "%*s\t%*d\t%*d\t%*s\t%*s\t%*s\t%*s\t%s", keybuffer); - if (numfields == 1) { - string keypairs = keybuffer; - string left = "TRIM_LEFT="; - string right = "TRIM_RIGHT="; - long int trim_l = 0; - long int trim_r = 0; - - // look for a trim_left field - size_t found = keypairs.find(left); - if (found != string::npos){ - string r1 = keypairs; - r1.erase(0, found+left.size()); - trim_l = strtol (r1.c_str(), NULL, 0); - } + target.hotspots_only = 0; + if (ParseInfoKey("HS_ONLY=", info, temp)) + target.hotspots_only = temp; - // look for a trim_right field - found = keypairs.find(right); - if (found != string::npos){ - string r1 = keypairs; - r1.erase(0, found+right.size()); - trim_r = strtol (r1.c_str(), NULL, 0); - } - assert(trim_l>=0); - assert(trim_r>=0); - target.trim_left = trim_l; - target.trim_right = trim_r; + target.read_mismatch_limit = -1; + if (ParseInfoKey("READ_MISMATCH_LIMIT=", info, temp)){ + target.read_mismatch_limit = temp; } - // cout << "trim assigned to amplicon starting " << target.begin << " with trimming " << target.trim_left << " and " << target.trim_right << endl; } +// ------------------------------------------------------------------------------------- +// expects a zero-based position to match target indexing. -void TargetsManager::TrimAmpliseqPrimers(Alignment *rai, int unmerged_target_hint) const +int TargetsManager::ReportHotspotsOnly(const MergedTarget &merged, int chr, long pos0) { + // Verify that (chr,pos) is inside the merged target + if (merged.chr != chr or pos0 < merged.begin or pos0 >= merged.end){ + cerr << "ReportHotspotsOnly ERROR: (chr=" << chr << ",pos=" << pos0 << ") not in target chr " + << merged.chr << ":" << merged.begin << "-" << merged.end << endl; + exit(1); + } + + // We take the first target index that contains the variant position, excluding primers. + unsigned int target_idx = merged.first_unmerged; + while (target_idx < unmerged.size() and unmerged[target_idx].end-unmerged[target_idx].trim_right < pos0) + ++target_idx; + + if (target_idx < unmerged.size() and pos0 >= unmerged[target_idx].begin) + return unmerged[target_idx].hotspots_only; + + return 0; +} + +// ------------------------------------------------------------------------------------- + +void TargetsManager::GetBestTargetIndex(Alignment *rai, int unmerged_target_hint, int& best_target_idx, int& best_fit_penalty, int& best_overlap) const +{ + // set these before any trimming rai->align_start = rai->alignment.Position; rai->align_end = rai->alignment.GetEndPosition(false, true); rai->old_cigar = rai->alignment.CigarData; - if (not trim_ampliseq_primers) - return; - // Step 1: Find the first potential target region int target_idx = unmerged_target_hint; @@ -271,10 +301,9 @@ void TargetsManager::TrimAmpliseqPrimers(Alignment *rai, int unmerged_target_hin // Step 2: Iterate over potential target regions, evaluate fit, pick the best fit - - int best_target_idx = -1; - int best_fit_penalty = 100; - int best_overlap = 0; + best_target_idx = -1; + best_fit_penalty = 100; + best_overlap = 0; while (target_idx < (int)unmerged.size() and rai->alignment.RefID == unmerged[target_idx].chr and rai->end >= unmerged[target_idx].begin) { @@ -285,16 +314,22 @@ void TargetsManager::TrimAmpliseqPrimers(Alignment *rai, int unmerged_target_hin int overlap = min(unmerged[target_idx].end, read_end) - max(unmerged[target_idx].begin, read_start); int fit_penalty = 100; + float overlap_ratio = (float) overlap / (float) (unmerged[target_idx].end - unmerged[target_idx].begin); + if (overlap_ratio > min_coverage_fraction) + rai->target_coverage_indices.push_back(target_idx); + if (not rai->alignment.IsReverseStrand()) { if (read_prefix_size > 0) fit_penalty = min(read_prefix_size,50) + max(0,50-overlap); else fit_penalty = min(-3*read_prefix_size,50) + max(0,50-overlap); + if (read_postfix_size > 30) fit_penalty += read_postfix_size-10; } else { if (read_postfix_size > 0) fit_penalty = min(read_postfix_size,50) + max(0,50-overlap); else fit_penalty = min(-3*read_postfix_size,50) + max(0,50-overlap); + if (read_prefix_size > 30) fit_penalty += read_prefix_size-10; } if (read_prefix_size > 0 and read_postfix_size > 0) fit_penalty -= 10; @@ -307,14 +342,34 @@ void TargetsManager::TrimAmpliseqPrimers(Alignment *rai, int unmerged_target_hin ++target_idx; } + if (rai->target_coverage_indices.size() > 1){ + sort(rai->target_coverage_indices.begin(), rai->target_coverage_indices.end()); + } +} + - if (best_target_idx == -1) { - rai->filtered = true; - return; +void TargetsManager::TrimAmpliseqPrimers(Alignment *rai, int unmerged_target_hint) const +{ + int best_target_idx = -1; + int best_fit_penalty = 100; + int best_overlap = 0; + GetBestTargetIndex(rai, unmerged_target_hint, best_target_idx, best_fit_penalty, best_overlap); + + if (best_target_idx < 0 or rai->target_coverage_indices.empty()){ + rai->filtered = true; + return; } + if (not trim_ampliseq_primers){ + return; + } - // Step 3: Do the actual primer trimming. + // Step 1: Find the best target index + if (best_target_idx < 0){ + rai->filtered = true; + return; + } + // Step 2: Do the actual primer trimming. // // For now, only adjust Position and Cigar. // Later, also adjust MD tag. @@ -326,7 +381,7 @@ void TargetsManager::TrimAmpliseqPrimers(Alignment *rai, int unmerged_target_hin vector::iterator old_op = old_cigar.begin(); int ref_pos = rai->alignment.Position; - // 3A: Cigar ops left of the target + // 2A: Cigar ops left of the target int begin = unmerged[best_target_idx].begin + unmerged[best_target_idx].trim_left; if (begin > unmerged[best_target_idx].end) @@ -373,8 +428,11 @@ void TargetsManager::TrimAmpliseqPrimers(Alignment *rai, int unmerged_target_hin if (old_op->Type == 'D') { if (old_op->Length > gap) { - old_op->Length -= gap; - ref_pos += gap; + //old_op->Length -= gap; + //ref_pos += gap; + // avoid #S#D case, extend align position, remove the leading D. + ref_pos += old_op->Length; + ++old_op; break; } else { ref_pos += old_op->Length; @@ -385,7 +443,7 @@ void TargetsManager::TrimAmpliseqPrimers(Alignment *rai, int unmerged_target_hin } - // 3B: Cigar ops in the middle of the target + // 2B: Cigar ops in the middle of the target rai->alignment.Position = ref_pos; @@ -419,13 +477,13 @@ void TargetsManager::TrimAmpliseqPrimers(Alignment *rai, int unmerged_target_hin } if (old_op->Type == 'D') { - new_cigar.push_back(CigarOp('D')); if (old_op->Length > gap) { - new_cigar.back().Length = gap; - old_op->Length -= gap; - ref_pos += gap; + // last D op, remove this one + ref_pos += old_op->Length; + ++old_op; break; } else { + new_cigar.push_back(CigarOp('D')); new_cigar.back().Length = old_op->Length; ref_pos += old_op->Length; ++old_op; @@ -434,8 +492,7 @@ void TargetsManager::TrimAmpliseqPrimers(Alignment *rai, int unmerged_target_hin } } - - // 3C: Cigar ops to the right of the target + // 2C: Cigar ops to the right of the target for (; old_op != old_cigar.end(); ++old_op) { if (old_op->Type == 'H' or old_op->Type == 'D') @@ -455,14 +512,72 @@ void TargetsManager::TrimAmpliseqPrimers(Alignment *rai, int unmerged_target_hin ZL << unmerged[best_target_idx].name << ":" << best_fit_penalty << ":" << best_overlap; rai->alignment.AddTag("ZL", "Z", ZL.str()); - - } +// Filter out a read if any of the following conditions is satisfied +// a) The read does not cover any region. +// b) The coverage ratio at the best region < min_coverage_ratio. +bool TargetsManager::FilterReadByRegion(Alignment* rai, int unmerged_target_hint) const +{ + bool is_filtered_out = false; + int best_target_idx = -1; + int best_fit_penalty = 100; + int best_overlap = 0; + GetBestTargetIndex(rai, unmerged_target_hint, best_target_idx, best_fit_penalty, best_overlap); + + // Filter out the read if it does not cover any region. + if (best_target_idx < 0 or rai->target_coverage_indices.empty()){ + is_filtered_out = true; + rai->filtered = true; + return is_filtered_out; + } + + return is_filtered_out; +} +void TargetsManager::WriteTargetsCoverage(const string& target_cov_file, const ReferenceReader& ref_reader) const { + ofstream target_cov_out; + target_cov_out.open(target_cov_file.c_str(), ofstream::out); + target_cov_out << "chr" << "\t" + << "pos_start" << "\t" + << "pos_end" << "\t" + << "name" << "\t" + << "read_depth" << "\t" + << "family_depth" << "\t" + << "fam_size_hist" << endl; + + for (vector::const_iterator target_it = unmerged.begin(); target_it != unmerged.end(); ++target_it){ + unsigned int check_read_cov = 0; + unsigned int check_fam_cov = 0; + target_cov_out << ref_reader.chr_str(target_it->chr) << "\t" + << target_it->begin << "\t" + << target_it->end << "\t" + << (target_it->name.empty()? "." : target_it->name) << "\t" + << target_it->my_stat.read_coverage << "\t" + << target_it->my_stat.family_coverage << "\t"; + for (map::const_iterator hist_it = target_it->my_stat.fam_size_hist.begin(); hist_it != target_it->my_stat.fam_size_hist.end(); ++hist_it){ + target_cov_out << "(" << hist_it->first << "," << hist_it->second <<"),"; + check_fam_cov += hist_it->second; + check_read_cov += (hist_it->second * (unsigned int) hist_it->first); + } + target_cov_out << endl; + // Check fam_size_hist matches read/family coverages. + assert((check_read_cov == target_it->my_stat.read_coverage) and (check_fam_cov == target_it->my_stat.family_coverage)); + } + target_cov_out.close(); +} - - - +void TargetsManager::AddCoverageToRegions(const map& stat_of_targets){ + pthread_mutex_lock(&coverage_counter_mutex_); + for (map::const_iterator stat_it = stat_of_targets.begin(); stat_it != stat_of_targets.end(); ++stat_it){ + int target_idx = stat_it->first; + unmerged[target_idx].my_stat.read_coverage += stat_it->second.read_coverage; + unmerged[target_idx].my_stat.family_coverage += stat_it->second.family_coverage; + for (map::const_iterator hist_it = stat_it->second.fam_size_hist.begin(); hist_it != stat_it->second.fam_size_hist.end(); ++hist_it){ + unmerged[target_idx].my_stat.fam_size_hist[hist_it->first] += hist_it->second; + } + } + pthread_mutex_unlock(&coverage_counter_mutex_); +} diff --git a/Analysis/VariantCaller/TargetsManager.h b/Analysis/VariantCaller/TargetsManager.h index 67b654ee..f75ad317 100644 --- a/Analysis/VariantCaller/TargetsManager.h +++ b/Analysis/VariantCaller/TargetsManager.h @@ -11,6 +11,8 @@ #include #include #include +#include +#include #include "ReferenceReader.h" struct Alignment; @@ -22,33 +24,51 @@ struct MergedTarget { int first_unmerged; }; +struct TargetStat{ + unsigned int read_coverage = 0; + unsigned int family_coverage = 0; + // fam_size_hist[x] = y indicates there are y families of size x. + map fam_size_hist; +}; + class TargetsManager { public: TargetsManager(); ~TargetsManager(); - void Initialize(const ReferenceReader& ref_reader, const string& _targets, bool _trim_ampliseq_primers = false); - + void Initialize(const ReferenceReader& ref_reader, const string& _targets, float min_cov_frac = 0.0f, bool _trim_ampliseq_primers = false); struct UnmergedTarget { - int chr; - int begin; - int end; - string name; - int merged; - int trim_left; - int trim_right; + int chr = 0; + int begin = 0; + int end = 0; + string name = ""; + int merged = 0; + int trim_left = 0; + int trim_right = 0; + int hotspots_only = 0; + int read_mismatch_limit = -1; + TargetStat my_stat; }; void LoadRawTargets(const ReferenceReader& ref_reader, const string& bed_filename, list& raw_targets); - void AddExtraTrim(UnmergedTarget& target, char *region_name, int num_fields); + void ParseBedInfoField(UnmergedTarget& target, const string info); void TrimAmpliseqPrimers(Alignment *rai, int unmerged_target_hint) const; + void GetBestTargetIndex(Alignment *rai, int unmerged_target_hint, int& best_target_idx, int& best_fit_penalty, int& best_overlap) const; + bool FilterReadByRegion(Alignment* rai, int unmerged_target_hint) const; + void AddCoverageToRegions(const map& stat_of_targets); + void WriteTargetsCoverage(const string& file_path, const ReferenceReader& ref_reader) const; + int ReportHotspotsOnly(const MergedTarget &merged, int chr, long pos); vector unmerged; vector merged; bool trim_ampliseq_primers; + // The following variables are just for bool FilterReadByRegion(Alignment* rai, int recent_target) use only. + float min_coverage_fraction; +private: + pthread_mutex_t coverage_counter_mutex_; }; diff --git a/Analysis/VariantCaller/VariantCaller.cpp b/Analysis/VariantCaller/VariantCaller.cpp index bf7d04ac..05b838a4 100644 --- a/Analysis/VariantCaller/VariantCaller.cpp +++ b/Analysis/VariantCaller/VariantCaller.cpp @@ -33,8 +33,8 @@ #include "tvcutils/unify_vcf.h" #include "IndelAssembly/IndelAssembly.h" - #include "MolecularTag.h" +#include "Consensus.h" using namespace std; @@ -48,9 +48,13 @@ void TheSilenceOfTheArmadillos(ofstream &null_ostream) void * VariantCallerWorker(void *input); -int main(int argc, char* argv[]) -{ +// -------------------------------------------------------------------------------------------------------------- +// The tvc exectuable is currently overloaded and harbors "tvc consensus" within +// Below function is the classic tvc main function + +static int main_tvc(int argc, char* argv[]) +{ printf("tvc %s-%s (%s) - Torrent Variant Caller\n\n", IonVersion::GetVersion().c_str(), IonVersion::GetRelease().c_str(), IonVersion::GetGitHash().c_str()); @@ -60,37 +64,21 @@ int main(int argc, char* argv[]) time_t start_time = time(NULL); - + // Read parameters and create output directories ExtendParameters parameters(argc, argv); - - mkdir(parameters.outputDir.c_str(), S_IRWXU | S_IRWXG | S_IROTH | S_IXOTH); - - if (parameters.program_flow.rich_json_diagnostic || parameters.program_flow.minimal_diagnostic) { - // make output directory "side effect bad" - parameters.program_flow.json_plot_dir = parameters.outputDir + "/json_diagnostic/"; - mkdir(parameters.program_flow.json_plot_dir.c_str(), S_IRWXU | S_IRWXG | S_IROTH | S_IXOTH); - } - ReferenceReader ref_reader; ref_reader.Initialize(parameters.fasta); TargetsManager targets_manager; - targets_manager.Initialize(ref_reader, parameters.targets, parameters.trim_ampliseq_primers); + targets_manager.Initialize(ref_reader, parameters.targets, parameters.min_cov_fraction, parameters.trim_ampliseq_primers); BAMWalkerEngine bam_walker; bam_walker.Initialize(ref_reader, targets_manager, parameters.bams, parameters.postprocessed_bam, parameters.prefixExclusion); bam_walker.GetProgramVersions(parameters.basecaller_version, parameters.tmap_version); SampleManager sample_manager; - string sample_name = parameters.sampleName; - sample_manager.Initialize(bam_walker.GetBamHeader(), parameters.sampleName, parameters.force_sample_name); - if ((sample_name == "") and (sample_manager.num_samples_ > 1)) { - parameters.multisample = true; - cout << "Multisample run detected." << endl; - cerr << "FATAL ERROR: Multisample runs are not currently supported." << endl; - exit(-1); - } + sample_manager.Initialize(bam_walker.GetBamHeader(), parameters.sampleName, parameters.force_sample_name, parameters.multisample); InputStructures global_context; global_context.Initialize(parameters, ref_reader, bam_walker.GetBamHeader()); @@ -98,8 +86,16 @@ int main(int argc, char* argv[]) MolecularTagTrimmer tag_trimmer; tag_trimmer.InitializeFromSamHeader(parameters.tag_trimmer_parameters, bam_walker.GetBamHeader()); + MolecularTagManager mol_tag_manager; + mol_tag_manager.Initialize(&tag_trimmer, &sample_manager); + + if (tag_trimmer.HaveTags()){ + cout << "TVC: Call small variants with molecular tags. Indel Assembly will be turned off automatically." << endl; + parameters.program_flow.do_indel_assembly = false; + } + OrderedVCFWriter vcf_writer; - vcf_writer.Initialize(parameters.outputDir + "/" + parameters.outputFile, parameters, ref_reader, sample_manager); + vcf_writer.Initialize(parameters.outputDir + "/" + parameters.small_variants_vcf, parameters, ref_reader, sample_manager, tag_trimmer.HaveTags()); OrderedBAMWriter bam_writer; @@ -115,14 +111,18 @@ int main(int argc, char* argv[]) parsed_opts.setReference(parameters.fasta); parsed_opts.setBams(parameters.bams); parsed_opts.setTargetFile(parameters.targets); - parsed_opts.setOutputVcf(parameters.outputDir + "/indel_assembly.vcf"); + parsed_opts.setOutputVcf(parameters.outputDir + "/" + parameters.indel_assembly_vcf); parsed_opts.setParametersFile(parameters_file); - parsed_opts.setSampleName(sample_name); + // Weird behavior of assembly where an empty sample name enables multi-sample analysis + parsed_opts.setSampleName(parameters.multisample ? "" : sample_manager.primary_sample_name_); // Print the indel_assembly parameters if do_indel_assembly = true - if(parameters.program_flow.do_indel_assembly) + if(parameters.program_flow.do_indel_assembly){ + cout << "TVC: Parsing Indel Assembly parameters." << endl; parsed_opts.processParameters(parameters.opts); - else - cout<<"TVC: Indel assembly off."<< endl; + } + else{ + cout<<"TVC: Indel Assembly off."<< endl; + } IndelAssembly indel_assembly(&parsed_opts, &ref_reader, &sample_manager, &targets_manager); @@ -143,23 +143,7 @@ int main(int argc, char* argv[]) vc.metrics_manager = &metrics_manager; vc.sample_manager = &sample_manager; vc.indel_assembly = &indel_assembly; - vc.tag_trimmer = &tag_trimmer; - - // Will tvc call small variants with mol tags? - bool use_molecular_tag = vc.tag_trimmer->HaveTags(); - - if(use_molecular_tag){ - cout<< "TVC: Found "<< vc.tag_trimmer->NumTaggedReadGroups() << " read group(s) with molecular tags. Call small variants using molecular tags." << endl; - if(vc.tag_trimmer->NumReadGroups() > vc.tag_trimmer->NumTaggedReadGroups()){ - cout<< "TVC: Molecular tags not found in read group(s) {"; - for(int read_group_idx = 0; read_group_idx < vc.tag_trimmer->NumReadGroups(); ++read_group_idx){ - if(not vc.tag_trimmer->HasTags(read_group_idx)){ - cout<program_flow.do_indel_assembly){ - indel_assembly.onTraversalDone(); - } - else{ - indel_assembly.out.close(); - } - - string novel_vcf = parameters.outputDir + "/" + parameters.outputFile; - string assembly_vcf = parameters.outputDir + "/indel_assembly.vcf"; - string hotspots_file = parameters.variantPriorsFile; - string output_vcf = parameters.outputDir + "/TSVC_variants.vcf"; - string tvc_metrics = parameters.outputDir + "/tvc_metrics.json"; - string input_depth = parameters.outputDir + "/depth.txt"; - string output_genome_vcf = parameters.outputDir + "/TSVC_variants.genome.vcf"; + // --- Indel Assembly - if (!parameters.postprocessed_bam.empty()) { - int return_code = system(string("samtools index " + parameters.postprocessed_bam).c_str()); - } + indel_assembly.onTraversalDone(vc.parameters->program_flow.do_indel_assembly); + cout << "[tvc] Finished indel assembly. Processing time: " << (time(NULL)-indel_start_time) << " seconds." << endl; - { // block serves as isolation of merging and building tabix index - // Prepare merger object - VcfOrderedMerger merger(novel_vcf, assembly_vcf, hotspots_file, output_vcf, tvc_metrics, input_depth, output_genome_vcf, ref_reader, targets_manager, 10, max(0, parameters.minCoverage), true); + // --- VCF post processing & merging steps + /*/ XXX Not being done in TVC 5.4 but in the variant caller pipeline - merger.perform(); - } + time_t post_proc_start = time(NULL); - build_index(parameters.outputDir + "/TSVC_variants.vcf"); - build_index(parameters.outputDir + "/TSVC_variants.genome.vcf"); + string small_variants_vcf = parameters.outputDir + "/" + parameters.small_variants_vcf; + string indel_assembly_vcf = parameters.outputDir + "/" + parameters.indel_assembly_vcf; + string hotspots_file = parameters.variantPriorsFile; + string merged_vcf = parameters.outputDir + "/" + parameters.merged_vcf; + string tvc_metrics = parameters.outputDir + "/tvc_metrics.json"; // Name hard coded in metrics manager FinalizeAndSave call + string input_depth = parameters.outputDir + "/depth.txt"; // Name hard coded in BAM walker initialization + string output_genome_vcf = parameters.outputDir + "/" + parameters.merged_genome_vcf; - cerr << endl; - cout << endl; - cout << "[tvc] Normal termination. Processing time: " << (time(NULL)-start_time) << " seconds." << endl; + // VCF merging & post processing filters / subset annotation + VcfOrderedMerger merger(small_variants_vcf, indel_assembly_vcf, hotspots_file, merged_vcf, tvc_metrics, input_depth, + output_genome_vcf, ref_reader, targets_manager, 10, max(0, parameters.minCoverage), true); + merger.SetVCFrecordFilters(parameters.my_controls.filter_by_target, parameters.my_controls.hotspot_positions_only, parameters.my_controls.hotspot_variants_only); + merger.perform(); - return 0; -} + build_index(merged_vcf); + build_index(output_genome_vcf); + cout << "[tvc] Finished vcf post processing. Processing time: " << (time(NULL)-post_proc_start) << " seconds." << endl; + // */ + cerr << endl; + cout << endl; + cout << "[tvc] Total Processing time: " << (time(NULL)-start_time) << " seconds." << endl; + return EXIT_SUCCESS; +} +// -------------------------------------------------------------------------------------------------------------- +// The tvc exectuable is currently overloaded and harbors "tvc consensus" +int main(int argc, char* argv[]) +{ + if (argc > 1){ + if (string(argv[1]) == "consensus"){ + return ConsensusMain(argc - 1, argv + 1); + } + } + return main_tvc(argc, argv); +} +// -------------------------------------------------------------------------------------------------------------- void * VariantCallerWorker(void *input) { - Consensus consensus; - list consensus_position; - consensus_position.push_back(PositionInProgress()); - list::iterator new_position_ticket = consensus_position.begin(); - new_position_ticket->begin = NULL; - new_position_ticket->end = NULL; BamAlignment alignment; VariantCallerContext& vc = *static_cast(input); @@ -270,17 +258,31 @@ void * VariantCallerWorker(void *input) Alignment * new_read[kReadBatchSize]; bool success[kReadBatchSize]; list::iterator position_ticket; + int prev_positioin_ticket_begin = -1; // position_ticket.begin at the previous while loop + int prev_positioin_ticket_end = -1; // position_ticket.end at the previous while loop PersistingThreadObjects thread_objects(*vc.global_context); + + Consensus consensus; + consensus.SetFlowConsensus(false); + list consensus_position_temp(1); + list::iterator consensus_position_ticket = consensus_position_temp.begin(); + consensus_position_ticket->begin = NULL; + consensus_position_ticket->end = NULL; + + CandidateExaminer my_examiner(&thread_objects, &vc); + bool more_positions = true; - bool use_molecular_tag = vc.tag_trimmer->HaveTags(); + // Molecular tagging related stuffs + const bool use_molecular_tag = vc.mol_tag_manager->tag_trimmer->HaveTags(); + vector< vector< vector > > my_molecular_families_multisample; + MolecularFamilyGenerator my_family_generator; pthread_mutex_lock(&vc.bam_walker_mutex); MetricsAccumulator& metrics_accumulator = vc.metrics_manager->NewAccumulator(); pthread_mutex_unlock(&vc.bam_walker_mutex); while (true /*more_positions*/) { - - // Opportunistic read removal + // Opportunistic read removal if (vc.bam_walker->EligibleForReadRemoval()) { if (pthread_mutex_trylock(&vc.read_removal_mutex) == 0) { @@ -374,24 +376,33 @@ void * VariantCallerWorker(void *input) } pthread_mutex_unlock(&vc.read_loading_mutex); - + bool more_read_come = false; for (int i = 0; i < kReadBatchSize and success[i]; ++i) { // 1) Filling in read body information and do initial set of read filters if (not vc.candidate_generator->BasicFilters(*new_read[i])) continue; // 2) Alignment information altering methods (can also filter a read) - if (not vc.tag_trimmer->GetTagsFromBamAlignment(new_read[i]->alignment, new_read[i]->tag_info)){ + if (not vc.mol_tag_manager->tag_trimmer->GetTagsFromBamAlignment(new_read[i]->alignment, new_read[i]->tag_info)){ new_read[i]->filtered = true; continue; } + + // 3) Filter by target and Trim ampliseq primer here vc.targets_manager->TrimAmpliseqPrimers(new_read[i], vc.bam_walker->GetRecentUnmergedTarget()); if (new_read[i]->filtered) continue; - // 3) Parsing alignment: Read filtering & populating allele specific data types in Alignment object + + // 4) Filter by read mismatch limit (note: NOT the filter for read-max-mismatch-fraction) here. + FilterByModifiedMismatches(new_read[i], vc.parameters->read_mismatch_limit, vc.targets_manager); + if (new_read[i]->filtered) + continue; + + // 5) Parsing alignment: Read filtering & populating allele specific data types in Alignment object vc.candidate_generator->UnpackReadAlleles(*new_read[i]); - // 4) Unpacking read meta data for evaluator + + // 6) Unpacking read meta data for evaluator UnpackOnLoad(new_read[i], *vc.global_context); } @@ -419,84 +430,55 @@ void * VariantCallerWorker(void *input) vc.bam_walker->BeginPositionProcessingTask(position_ticket); pthread_mutex_unlock(&vc.bam_walker_mutex); - //@TODO: Still not the optimal way to do this. - //@TODO: my_molecular_families_multisample should be dynamically updated with BAMWalkerEngine when we add or remove a read. - //@TODO: We should declare vector< vector< map > > > my_molecular_families_multisample; - // Gather the reads into families - // my_molecular_families_multisample.size() = # of samples - // my_molecular_families_multisample[sample_index].size() = 2 strands, 0 for fwd, 1 for rev - // my_molecular_families_multisample[sample_index][strand_key].size() # of families found on the strand - vector< vector< vector > > > my_molecular_families_multisample; - int sample_num = 1; if(use_molecular_tag){ + int sample_num = 1; if (vc.parameters->multisample){ sample_num = vc.sample_manager->num_samples_; } - my_molecular_families_multisample.clear(); - my_molecular_families_multisample.resize(sample_num); - - for (int sample_index = 0; (sample_index < sample_num); ++sample_index) { - GenerateMyMolecularFamilies(*position_ticket, my_molecular_families_multisample[sample_index], *(vc.parameters), sample_index); - for (vector > >::iterator iter = my_molecular_families_multisample[sample_index].begin(); (iter != my_molecular_families_multisample[sample_index].end()); ++iter) { - for (vector >::iterator iter2 = iter->begin(); (iter2 != iter->end()); ++iter2) { - if (iter2->family_members.size() >= (unsigned int)vc.parameters->tag_trimmer_parameters.min_family_size) { - Alignment* alignment = new Alignment; - consensus.CalculateConsensus(*vc.ref_reader, iter2->family_members, *alignment); - if (not vc.candidate_generator->BasicFilters(*alignment)) {delete alignment;} - else { - vc.targets_manager->TrimAmpliseqPrimers(alignment, vc.bam_walker->GetRecentUnmergedTarget()); - if (alignment->filtered) {delete alignment;} - else { - if (new_position_ticket->begin == NULL) {new_position_ticket->begin = alignment;} - if (new_position_ticket->end != NULL) {new_position_ticket->end->next = alignment;} - new_position_ticket->end = alignment; - } - } - } - } - } + // No need to generate families if position_ticket is not changed. + bool is_position_ticket_changed = true; + if (position_ticket->begin != NULL and position_ticket->end != NULL){ + is_position_ticket_changed = (prev_positioin_ticket_begin != position_ticket->begin->read_number) or (prev_positioin_ticket_end != position_ticket->end->read_number); + } + + if (is_position_ticket_changed){ + for (int sample_index = 0; sample_index < sample_num; ++sample_index) { + int overloaded_sample_index = vc.parameters->multisample ? sample_index : -1; + my_molecular_families_multisample.resize(sample_num); + my_family_generator.GenerateMyMolecularFamilies(vc.mol_tag_manager, *position_ticket, overloaded_sample_index, my_molecular_families_multisample[sample_index]); + } + GenerateConsensusPositionTicket(my_molecular_families_multisample, vc, consensus, consensus_position_ticket); + } + if (position_ticket->begin != NULL and position_ticket->end != NULL){ + prev_positioin_ticket_begin = position_ticket->begin->read_number; + prev_positioin_ticket_end = position_ticket->end->read_number; + }else{ + prev_positioin_ticket_begin = -1; + prev_positioin_ticket_end = -1; } - new_position_ticket->end = NULL; } + // Candidate Generation int haplotype_length = 1; - if (new_position_ticket->begin != NULL) { - Alignment* p = new_position_ticket->begin; - while ((p != NULL) and (p != new_position_ticket->end)) { + if(consensus_position_ticket->begin != NULL){ + for (Alignment *p = consensus_position_ticket->begin; p; p = p->next) { vc.candidate_generator->UnpackReadAlleles(*p); - p = p->next; } - vc.bam_walker->SetupPositionTicket(new_position_ticket); - vc.candidate_generator->GenerateCandidates(variant_candidates, new_position_ticket, haplotype_length); - while ((new_position_ticket->begin != NULL) and (new_position_ticket->begin != new_position_ticket->end)) { - p = new_position_ticket->begin; - new_position_ticket->begin = new_position_ticket->begin->next; + vc.bam_walker->SetupPositionTicket(consensus_position_ticket); + vc.candidate_generator->GenerateCandidates(variant_candidates, consensus_position_ticket, haplotype_length); + while ((consensus_position_ticket->begin != NULL) and (consensus_position_ticket->begin != consensus_position_ticket->end)) { + Alignment* p = consensus_position_ticket->begin; + consensus_position_ticket->begin = consensus_position_ticket->begin->next; delete p; } -/* - if ((variant_candidates.size() > 0) and (variant_candidates[0].variant.position == 55259515)) { - p = new_position_ticket->begin; - while ((p != NULL) and (p != new_position_ticket->end)) { - //if (p->alignment.Name == "TSJAM:02884:02345") { - string ref_string = ""; - ReferenceReader::iterator ref_ptr = vc.ref_reader->iter(p->alignment.RefID, p->alignment.Position); - for (unsigned int i = 0; (i < p->alignment.QueryBases.length()); ++i) { - ref_string += *ref_ptr; - ++ref_ptr; - } - if (ref_string != p->alignment.QueryBases) { - cerr << p->alignment.Name << endl; - cerr << "consensus\t" << p->alignment.Position << "\t" << p->alignment.QueryBases << endl; - cerr << "reference\t" << p->alignment.Position << "\t" << ref_string << endl; - cerr << endl; - } - //} - } - } -*/ + if (consensus_position_ticket->begin != NULL) { + delete consensus_position_ticket->begin; + consensus_position_ticket->begin = NULL; + consensus_position_ticket->end = NULL; + } } - else { - vc.candidate_generator->GenerateCandidates(variant_candidates, position_ticket, haplotype_length); + else{ + vc.candidate_generator->GenerateCandidates(variant_candidates, position_ticket, haplotype_length, &my_examiner); } pthread_mutex_lock(&vc.bam_walker_mutex); @@ -532,9 +514,11 @@ void * VariantCallerWorker(void *input) if (vc.parameters->multisample) { bool pass = false; // if pass == false the no reads for the candidate bool filter = true; + int evaluator_error_code = 0; for (int sample_index = 0; (sample_index < vc.sample_manager->num_samples_); ++sample_index) { - if (EnsembleProcessOneVariant(thread_objects, vc, *v, *position_ticket, - my_molecular_families_multisample[sample_index], sample_index)) {pass = true;} + evaluator_error_code = EnsembleProcessOneVariant(thread_objects, vc, *v, *position_ticket, my_molecular_families_multisample[sample_index], sample_index); + pass = evaluator_error_code == 0; + //TODO: czb: the logic here is a mess. Need clean-up! if (!v->variant.isFiltered) {filter = false;} v->variant.isFiltered = false; } @@ -548,14 +532,17 @@ void * VariantCallerWorker(void *input) } if (!pass) { for (int sample_index = 0; (sample_index < vc.sample_manager->num_samples_); ++sample_index) { - AutoFailTheCandidate(v->variant, vc.parameters->my_controls.use_position_bias, v->variant.sampleNames[sample_index], use_molecular_tag); + string my_reason = evaluator_error_code == 2? "NOVALIDFUNCFAM" : "NODATA"; + AutoFailTheCandidate(v->variant, vc.parameters->my_controls.use_position_bias, v->variant.sampleNames[sample_index], use_molecular_tag, my_reason); } } } else { - if (!EnsembleProcessOneVariant(thread_objects, vc, *v, *position_ticket, - my_molecular_families_multisample[0], 0)) { - AutoFailTheCandidate(v->variant, vc.parameters->my_controls.use_position_bias, v->variant.sampleNames[0], use_molecular_tag); + // We only call based on the reads of the primary sample if a sample name was provided + int evaluator_error_code = EnsembleProcessOneVariant(thread_objects, vc, *v, *position_ticket, my_molecular_families_multisample[0], vc.sample_manager->primary_sample_); + if (evaluator_error_code != 0) { + string my_reason = evaluator_error_code == 2? "NOVALIDFUNCFAM" : "NODATA"; + AutoFailTheCandidate(v->variant, vc.parameters->my_controls.use_position_bias, v->variant.sampleNames[0], use_molecular_tag, my_reason); } } //v->isFiltered = true; diff --git a/Analysis/VariantCaller/tmol/tmol.cpp b/Analysis/VariantCaller/tmol/tmol.cpp index 4b638fef..6b7d2e4c 100644 --- a/Analysis/VariantCaller/tmol/tmol.cpp +++ b/Analysis/VariantCaller/tmol/tmol.cpp @@ -30,7 +30,7 @@ inline string itos(int i){ stringstream s; s << i; return s.str();} inline string ctos(char * c, int len){ stringstream s; for(int i=0;i1) allele_type = 3; // mnp=3 if(faf >= min_freq) { @@ -1401,8 +1402,8 @@ void report_consensus_counts(string & ref_seq, string ref_name, map0.999?"1/1:":"0/1:") << cons_sz[i] << ":" << global_consensus[12*i+j] << ":" << af << ":"; + if(filter_out)var_calls << ref_name << "\t" << (start_pos+i+1) << "\t.\t" << ref_allele << "\t" << alt_allele << "\t" << qual << "\tNOCALL\tDP=" << cons_sz[i] << ";AO=" << global_consensus[12*i+j] << ";AF=" << af << ";MDP=" << functional_counts << ";MAO=" << alt_key->second << ";MAF=" << faf << ";LOD=" << output_lod << ";TYPE=" << vartypes[allele_type] << ";FR=systematic_error\tGT:DP:AO:AF:FS\t" << "./.:" << cons_sz[i] << ":" << global_consensus[12*i+j] << ":" << af << ":"; + else var_calls << ref_name << "\t" << (start_pos+i+1) << "\t.\t" << ref_allele << "\t" << alt_allele << "\t" << qual << "\tPASS\tDP=" << cons_sz[i] << ";AO=" << global_consensus[12*i+j] << ";AF=" << af << ";MDP=" << functional_counts << ";MAO=" << alt_key->second << ";MAF=" << faf << ";LOD=" << output_lod << ";TYPE=" << vartypes[allele_type] << "\tGT:DP:AO:AF:FS\t" << (faf>0.999?"1/1:":"0/1:") << cons_sz[i] << ":" << global_consensus[12*i+j] << ":" << af << ":"; for(int k=0;k<9;k++) var_calls << functional_pileup[i][k].get_allele_read_counts(alt_key->first) << ","; var_calls << functional_pileup[i][9].get_allele_read_counts(alt_key->first) << endl; reported = true; @@ -1410,7 +1411,7 @@ void report_consensus_counts(string & ref_seq, string ref_name, map 0 && strcmp(hotspot_rec[h-1].get_ref(0).c_str(),ref_allele.c_str()) == 0 && strcmp(hotspot_rec[h-1].get_alt(0).c_str(),alt_allele.c_str()) == 0) { - var_calls << ref_name << "\t" << (start_pos+i+1) << "\t.\t" << ref_allele << "\t" << alt_allele << "\t" << 0 << "\tNOCALL\tDP=" << cons_sz[i] << ";AO=" << global_consensus[12*i+j] << ";AF=" << af << ";MDP=" << functional_counts << ";MAO=" << alt_key->second << ";MAF=" << faf << ";LOD=" << output_lod << ";FR=MAF<"<< faf <<";HS\tGT:DP:AO:AF:FS\t" << "./.:" << cons_sz[i] << ":" << global_consensus[12*i+j] << ":" << af << ":"; + var_calls << ref_name << "\t" << (start_pos+i+1) << "\t.\t" << ref_allele << "\t" << alt_allele << "\t" << 0 << "\tNOCALL\tDP=" << cons_sz[i] << ";AO=" << global_consensus[12*i+j] << ";AF=" << af << ";MDP=" << functional_counts << ";MAO=" << alt_key->second << ";MAF=" << faf << ";LOD=" << output_lod << ";FR=MAF<"<< faf << ";TYPE=" << vartypes[allele_type] <<";HS\tGT:DP:AO:AF:FS\t" << "./.:" << cons_sz[i] << ":" << global_consensus[12*i+j] << ":" << af << ":"; for(int k=0;k<9;k++) var_calls << functional_pileup[i][k].get_allele_read_counts(alt_key->first) << ","; var_calls << functional_pileup[i][9].get_allele_read_counts(alt_key->first) << endl; reported = true; @@ -1419,9 +1420,15 @@ void report_consensus_counts(string & ref_seq, string ref_name, maphotspot_rec[h-1].get_alt(a).length()?0:(hotspot_rec[h-1].get_ref(a).length()1) allele_type = 3; // mnp + if((allele_type==0 && (hotspot_rec[h-1].get_alt(a).length()>1 || hotspot_rec[h-1].get_alt(a)[0]!=hotspot_rec[h-1].get_ref(a)[0])) || (allele_type==2 && (hotspot_rec[h-1].get_ref(a).length()>1 || hotspot_rec[h-1].get_alt(a)[0]!=hotspot_rec[h-1].get_ref(a)[0]))) allele_type = 4; // complex + var_calls << ref_name << "\t" << (start_pos+i+1) << "\t.\t" << hotspot_rec[h-1].get_ref(a) << "\t" << hotspot_rec[h-1].get_alt(a) << "\t" << 0 << "\tREF\tDP=" << cons_sz[i] << ";AO=.;AF=.;MDP=" << functional_counts << ";MAO=.;MAF=.;LOD=" << output_lod << ";TYPE=" << vartypes[allele_type] << ";HS\tGT:DP:AO:AF:FS\t" << "0/0:" << cons_sz[i] << ":.:.:"; for(int k=0;k<9;k++) var_calls << functional_pileup[i][k].get_allele_read_counts(ref_seq.substr(i,1)) << ","; var_calls << functional_pileup[i][9].get_allele_read_counts(ref_seq.substr(i,1)) << endl; + } } } } @@ -1703,13 +1710,19 @@ int main(int argc, char *argv[]) var_calls << "##fileformat=VCFv4.1" << endl; var_calls << "##FORMAT=" << endl; var_calls << "##FORMAT=" << endl; - var_calls << "##INFO=" << endl; + var_calls << "##FORMAT=" << endl; + var_calls << "##FORMAT=" << endl; + var_calls << "##FORMAT=" << endl; + var_calls << "##INFO=" << endl; var_calls << "##INFO=" << endl; var_calls << "##INFO=" << endl; var_calls << "##INFO=" << endl; var_calls << "##INFO=" << endl; var_calls << "##INFO=" << endl; var_calls << "##INFO=" << endl; + var_calls << "##INFO=" << endl; + var_calls << "##INFO=" << endl; + var_calls << "##INFO=" << endl; var_calls << "##Tagged Molecule Caller tool v." << full_version_string << endl; var_calls << "##Tagging Method=" << tag_method << endl; var_calls << "##Tagging Level=" << tag_level << endl; diff --git a/Analysis/VariantCaller/tvcutils/prepare_hotspots.cpp b/Analysis/VariantCaller/tvcutils/prepare_hotspots.cpp index 7de47e9a..bea2e74b 100644 --- a/Analysis/VariantCaller/tvcutils/prepare_hotspots.cpp +++ b/Analysis/VariantCaller/tvcutils/prepare_hotspots.cpp @@ -8,6 +8,7 @@ #include #include #include +#include #include #include #include @@ -34,6 +35,8 @@ void PrepareHotspotsHelp() printf ("General options:\n"); printf (" -b,--input-bed FILE input is a hotspots BED file (either -b or -v required)\n"); printf (" -v,--input-vcf FILE input is a hotspots VCF file (either -b or -v required)\n"); + printf (" -p,--input-real-vcf FILE input is a real vcf file that we will process (when this is present -b and -v cannot, but -o must present)\n"); + printf (" -q,--output-fake-hot-vcf FILE output a hotspot file for subset alleles\n"); printf (" -d,--output-bed FILE output left-aligned hotspots in BED format [none]\n"); printf (" -o,--output-vcf FILE output is a hotspots VCF file. To be used as input to --hotspot-vcf argument of variant_caller_pipeline.py (recommended) [none]\n"); printf (" -r,--reference FILE FASTA file containing reference genome (required)\n"); @@ -221,12 +224,126 @@ class junction { vector junc_; }; +static bool is_mnp_indel(const char *r, int rl, const char *a, int al) +{ + if (rl == al) return true; + int i, j, k; + for (i = 0; i < rl and i < al; i++) if (r[i] != a[i]) break; + for (j = rl-1, k = al-1; j >=i and k >= i; j--, k--) if (r[j] != a[k]) break; + if (j < i or k < i) return true; + return false; +} + +bool allele_subset(int pos1, const char *ref1, const char *alt1, int pos2, const char *ref2, const char *alt2) +{ + int rlen1 = strlen(ref1); + int rlen2 = strlen(ref2); + if (pos1+rlen1 < pos2+rlen2) return false; + if (pos1 > pos2) return false; + // pos1 <= pos2 + int alen1 = strlen(alt1), alen2 = strlen(alt2); + if (strncmp(ref1+pos2-pos1, ref2, rlen2)!=0) return false; // not even right + const char *s = strstr(alt1, alt2); + while (s) { + // check left right portion + if (is_mnp_indel(ref1, pos2-pos1, alt1, s-alt1) and is_mnp_indel(ref1+pos2-pos1+rlen2, pos1+rlen1-pos2-rlen2, alt1+(s-alt1+alen2), alen1-(s-alt1+alen2))) return true; + s = strstr(s+1, alt2); // check if there are multiple occurence of the small variant + } + return false; +} + + +class one_vcfline { //ZZ for subset + public: + one_vcfline(char *r, char *alt, int p, int g1, int g2, char *line) { + strcpy(ref, r); + //split alt into alts + char *ss; + for (ss = strtok(alt, ","); ss; ss = strtok(NULL, ",")) { + alts.push_back(string(ss)); + } + pos = p; + affected = false; + gt1 = g1; gt2 = g2; + o_line = string(line); + }; + char ref[1024]; + vector alts; + int pos; + bool affected; + void check_subset(one_vcfline &newline) { + unsigned int i, j; + bool need_pad = false; + for (i = 0; i < newline.alts.size(); ) { + if (i+1 != newline.gt1 and i+1 != newline.gt2) {i++; continue;} + for (j = 0; j < alts.size(); j++) { + if (j+1 != gt1 and j+1 != gt2) continue; + if (allele_subset(pos, ref, alts[j].c_str(), newline.pos, newline.ref, newline.alts[i].c_str())) break; + if (newline.pos == pos+1 and strlen(ref) < strlen(newline.ref)) { + char tmp[1024], tmp2[1024]; + tmp2[1] = tmp[1] = 0; tmp[0] = tmp2[0] = ref[0]; strcat(tmp, newline.ref); strcat(tmp2, newline.alts[i].c_str()); + if (allele_subset(pos, tmp, tmp2, pos, ref, alts[j].c_str())) { need_pad = true;break;} + } + } + if (j < alts.size()) { + if (need_pad) { + padding_tail(newline.ref+strlen(ref)-1); + } + add_one(newline.pos, strlen(newline.ref), newline.alts[i]); + newline.remove_ith_alt(i); + affected = newline.affected = true; + // after erase, no need to do i++; + } else { + i++; + } + } + }; + void padding_tail(char *addition) { + strcat(ref, addition); + string s(addition); + for (unsigned int i = 0; i < alts.size(); i++) alts[i] += s; + }; + bool produce_hot_vcf(char *chr, FILE *fp, int &hot_n, FILE *hot_p) { // out to a file + if (not affected) {fprintf(fp, "%s", o_line.c_str()); return false;} + fprintf(hot_p,"%s\t%d\thotspot_%d\t%s\t", chr, pos, hot_n, ref); + hot_n++; + for (unsigned int i = 0; i < alts.size(); i++) { + if (i != 0) fprintf(hot_p, ","); + fprintf(hot_p, "%s", alts[i].c_str()); + } + fprintf(hot_p, "\t.\t.\t.\n"); + return true; + }; + bool produce_hot_vcf(char *chr, FILE *fp, int &hot_n) { + return produce_hot_vcf(chr, fp, hot_n, stdout); + } + void remove_ith_alt(int i) { + alts.erase(alts.begin()+i); + }; + unsigned int gt1, gt2; + protected: + void add_one(unsigned int p, unsigned int reflen, string &alt) { + string s; + unsigned int i; + for (i = pos; i < p; i++) s.push_back(ref[i-pos]); //padding front + // add middle + s += alt; + // padding back + for (i = p+reflen; i < pos+strlen(ref); i++) s.push_back(ref[i-pos]); + alts.push_back(s); + }; + string o_line; +}; + + int PrepareHotspots(int argc, const char *argv[]) { OptArgs opts; opts.ParseCmdLine(argc, argv); string input_bed_filename = opts.GetFirstString ('b', "input-bed", ""); string input_vcf_filename = opts.GetFirstString ('v', "input-vcf", ""); + string input_real_vcf_filename = opts.GetFirstString ('p', "input-real-vcf", ""); + string output_hot_vcf = opts.GetFirstString ('q', "output-fake-hot-vcf", ""); string output_bed_filename = opts.GetFirstString ('d', "output-bed", ""); string output_vcf_filename = opts.GetFirstString ('o', "output-vcf", ""); string reference_filename = opts.GetFirstString ('r', "reference", ""); @@ -236,11 +353,15 @@ int PrepareHotspots(int argc, const char *argv[]) bool allow_block_substitutions = opts.GetFirstBoolean('s', "allow-block-substitutions", true); opts.CheckNoLeftovers(); - if((input_bed_filename.empty() == input_vcf_filename.empty()) or + if((input_bed_filename.empty() == (input_vcf_filename.empty() and input_real_vcf_filename.empty())) or (output_bed_filename.empty() and output_vcf_filename.empty()) or reference_filename.empty()) { PrepareHotspotsHelp(); return 1; } + if ((not input_real_vcf_filename.empty()) and (output_vcf_filename.empty() or not input_vcf_filename.empty())) { + PrepareHotspotsHelp(); + return 1; + } // Populate chromosome list from reference.fai @@ -342,6 +463,13 @@ int PrepareHotspots(int argc, const char *argv[]) continue; } + // OID= table has special meaning + if (string::npos != string(line2).find("OID=")) { + line_status.push_back(LineStatus(line_number)); + line_status.back().filter_message_prefix = "Bed line contains OID="; + continue; + } + char *current_chr = strtok(line2, "\t\r\n"); char *current_start = strtok(NULL, "\t\r\n"); char *current_end = strtok(NULL, "\t\r\n"); @@ -406,6 +534,7 @@ int PrepareHotspots(int argc, const char *argv[]) for (char *pos = current_alt+4; *pos; ++pos) allele.alt += toupper(*pos); // here is the place to check the length of the hotspot cover the amplicon junction. ZZ + /* if (junc.contain(allele.chr_idx, allele.pos, (unsigned int) allele.ref.size())) { line_status.push_back(LineStatus(line_number)); line_status.back().filter_message_prefix = "hotspot BED line contain the complete overlapping region of two amplicon, the variant cannot be detected by tvc"; @@ -416,6 +545,7 @@ int PrepareHotspots(int argc, const char *argv[]) line_status.back().filter_message_prefix = "hotspot BED line is not contained in any amplicon, the variant cannot be detected by tvc"; continue; } + */ allele.filtered = false; line_status.push_back(LineStatus(line_number)); @@ -434,17 +564,50 @@ int PrepareHotspots(int argc, const char *argv[]) } - if (!input_vcf_filename.empty()) { - FILE *input = fopen(input_vcf_filename.c_str(),"r"); - if (!input) { - fprintf(stderr,"ERROR: Cannot open %s\n", input_vcf_filename.c_str()); - return 1; + if (!input_vcf_filename.empty() or !input_real_vcf_filename.empty()) { + + bool real_vcf = false; + FILE *input; + FILE *out_real = NULL; + FILE *out_hot = NULL; + int fake_ = 0; + int hn = 1; + if (!input_real_vcf_filename.empty()) { + real_vcf = true; + input = fopen(input_real_vcf_filename.c_str(),"r"); + if (!input) { + fprintf(stderr,"ERROR: Cannot open %s\n", input_real_vcf_filename.c_str()); + return 1; + } + out_real = fopen(output_vcf_filename.c_str(), "w"); + if (!out_real) { + fprintf(stderr,"ERROR: Cannot open %s\n", output_vcf_filename.c_str()); + return 1; + } + if (!output_hot_vcf.empty()) { + out_hot = fopen(output_hot_vcf.c_str(), "w"); + if (!out_hot) { + fprintf(stderr,"ERROR: Cannot open %s\n", output_hot_vcf.c_str()); + return 1; + } + } else out_hot = stdout; + fprintf(out_hot, "##fileformat=VCFv4.1\n##allowBlockSubstitutions=true\n#CHROM POS ID REF ALT QUAL FILTER INFO\n"); + } else { + input = fopen(input_vcf_filename.c_str(),"r"); + if (!input) { + fprintf(stderr,"ERROR: Cannot open %s\n", input_vcf_filename.c_str()); + return 1; + } } char line2[65536]; + char line3[65536]; int line_number = 0; bool line_overflow = false; + list vcflist; + + char last_chr[1024] = ""; while (fgets(line2, 65536, input) != NULL) { if (line2[0] and line2[strlen(line2)-1] != '\n' and strlen(line2) == 65535) { line_overflow = true; @@ -462,9 +625,12 @@ int PrepareHotspots(int argc, const char *argv[]) allow_block_substitutions = true; continue; } - if (line2[0] == '#') + if (line2[0] == '#') { + if (out_real) { fprintf(out_real, "%s", line2);} continue; + } + if (real_vcf) strcpy(line3, line2); char *current_chr = strtok(line2, "\t\r\n"); char *current_start = strtok(NULL, "\t\r\n"); char *current_id = strtok(NULL, "\t\r\n"); @@ -473,10 +639,13 @@ int PrepareHotspots(int argc, const char *argv[]) strtok(NULL, "\t\r\n"); // Ignore QUAL strtok(NULL, "\t\r\n"); // Ignore FILTER char *current_info = strtok(NULL, "\t\r\n"); + strtok(NULL, "\t\r\n"); + char *gt = strtok(NULL, "\t\r\n"); if (!current_chr or !current_start or !current_id or !current_ref or !current_alt) { line_status.push_back(LineStatus(line_number)); - line_status.back().filter_message_prefix = "Malformed hotspot VCF line: expected at least 5 fields"; + if (real_vcf) line_status.back().filter_message_prefix = "Malformed real VCF line: expected at least 5 fields"; + else line_status.back().filter_message_prefix = "Malformed hotspot VCF line: expected at least 5 fields"; continue; } @@ -505,9 +674,13 @@ int PrepareHotspots(int argc, const char *argv[]) // Process custom tags vector bstrand; vector hp_max_length; + string raw_oid; + string raw_omapalt; + string raw_oalt; + string raw_oref; + string raw_opos; + if (current_info) { - string raw_oid; - string raw_omapalt; string raw_bstrand; string raw_hp_max_length; for (char *next = strtok(current_info, ";"); next; next = strtok(NULL, ";")) { @@ -526,12 +699,18 @@ int PrepareHotspots(int argc, const char *argv[]) continue; if (strcmp(next, "FR") == 0) continue; - if (strcmp(next, "OPOS") == 0) + if (strcmp(next, "OPOS") == 0) { + raw_opos = value; continue; - if (strcmp(next, "OREF") == 0) + } + if (strcmp(next, "OREF") == 0) { + raw_oref = value; continue; - if (strcmp(next, "OALT") == 0) + } + if (strcmp(next, "OALT") == 0) { + raw_oalt = value; continue; + } if (strcmp(next, "OID") == 0) { raw_oid = value; continue; @@ -557,7 +736,33 @@ int PrepareHotspots(int argc, const char *argv[]) } - + if (real_vcf) { + //fprintf(stderr, "%s\n", gt); + if (gt == NULL) continue; + // get gt + int g1 = atoi(gt), g2; + gt = strchr(gt, '/'); + if (gt) g2 = atoi(gt+1); + else {fprintf(stderr, "GT not formatted right\n"); exit(1);} + //if (g1 == 0 and g2 == 0) continue; + unsigned int cur_pos = atoi(current_start); + one_vcfline newline(current_ref, current_alt, cur_pos, g1, g2, line3); + bool new_chr = false; + if (strcmp(current_chr, last_chr) != 0) { + new_chr = true; + } + while (not vcflist.empty()) { + if ((not new_chr) and vcflist.front().pos+strlen(vcflist.front().ref) > cur_pos) break; + if (vcflist.front().produce_hot_vcf(last_chr, out_real, hn, out_hot)) fake_++; + vcflist.pop_front(); + } + if (new_chr) strcpy(last_chr, current_chr); + for (list::iterator it = vcflist.begin(); it != vcflist.end(); it++) { + it->check_subset(newline); + } + if (not newline.alts.empty()) vcflist.push_back(newline); + continue; + } unsigned int allele_idx = 0; for (char *sub_alt = strtok(current_alt,","); sub_alt; sub_alt = strtok(NULL,",")) { @@ -597,8 +802,20 @@ int PrepareHotspots(int argc, const char *argv[]) } fclose(input); + if (real_vcf) { + while (not vcflist.empty()) { + if (vcflist.front().produce_hot_vcf(last_chr, out_real, hn, out_hot)) fake_++; + vcflist.pop_front(); + } + fclose(out_real); + fclose(out_hot); + if (fake_ > 0) + return 0; + else return 1; + } } + // Process by chromosome: // - Verify reference allele // - Left align @@ -637,6 +854,19 @@ int PrepareHotspots(int argc, const char *argv[]) for (deque::iterator A = alleles[chr_idx].begin(); A != alleles[chr_idx].end(); ++A) { + // check bed file + if (junc.contain(A->chr_idx, A->pos, (unsigned int) A->ref.size())) { + A->filtered = true; + A->line_status->filter_message_prefix = "hotspot BED line contain the complete overlapping region of two amplicon, the variant cannot be detected by tvc"; + continue; + } + if (not junc.contained_in_ampl(A->chr_idx, A->pos, (unsigned int) A->ref.size())) { + A->filtered = true; + A->line_status->filter_message_prefix = "hotspot BED line is not contained in any amplicon, the variant cannot be detected by tvc"; + continue; + } + + // Invalid characters bool valid = true; @@ -748,6 +978,7 @@ int PrepareHotspots(int argc, const char *argv[]) alt_end = alt_end_orig; } else { // left align the indel part, here either ref_end = 0 or alt_end = 0 + int opos = A->pos; while (A->pos > 0) { char nuc = ref_index[chr_idx].base(A->pos-1); if (ref_end > 0 and A->ref[ref_end-1] != nuc) @@ -759,10 +990,20 @@ int PrepareHotspots(int argc, const char *argv[]) A->pos--; } if (ref_end != ref_end_orig) { - // trailing part is aligned, the whole ref and alt need to be kept. + // trailing part is aligned, the whole ref and alt need to be kept. ZZ ref_end = A->ref.size(); alt_end = A->alt.size(); } + if (junc.contain(chr_idx, A->pos, ref_end) or not junc.contained_in_ampl(chr_idx, A->pos, ref_end)) { + // after left align the hotspot contain an overlap region, revert to the original ZZ + if (opos != A->pos) { + A->ref.erase(0, opos-A->pos); + A->alt.erase(0, opos-A->pos); + A->pos = opos; + ref_end = ref_end_orig; + alt_end = alt_end_orig; + } + } } } A->ref.resize(ref_end); diff --git a/Analysis/VariantCaller/tvcutils/split_vcf.cpp b/Analysis/VariantCaller/tvcutils/split_vcf.cpp index 58d6ca89..cccd67bb 100644 --- a/Analysis/VariantCaller/tvcutils/split_vcf.cpp +++ b/Analysis/VariantCaller/tvcutils/split_vcf.cpp @@ -220,10 +220,10 @@ void check_sample(FILE *input, vector& out_files, string& qual_field } if ((*tag_iter == "AO") or (*tag_iter == "DP") or - (*tag_iter == "MDP") or - (*tag_iter == "MAO") or - (*tag_iter == "MRO") or - (*tag_iter == "MAF") or + (*tag_iter == "MDP") or + (*tag_iter == "MAO") or + (*tag_iter == "MRO") or + (*tag_iter == "MAF") or (*tag_iter == "FAO") or (*tag_iter == "FDP") or (*tag_iter == "FRO") or @@ -350,4 +350,4 @@ int SplitVcf(int argc, const char *argv[]) } return 0; -} +} \ No newline at end of file diff --git a/Analysis/VariantCaller/tvcutils/unify_vcf.cpp b/Analysis/VariantCaller/tvcutils/unify_vcf.cpp index 95f9df47..dd4f8787 100644 --- a/Analysis/VariantCaller/tvcutils/unify_vcf.cpp +++ b/Analysis/VariantCaller/tvcutils/unify_vcf.cpp @@ -11,6 +11,7 @@ #include #include #include +#include #include #include #include @@ -34,6 +35,41 @@ #define MAX_DP "MAX_DP" #define MIN_DP "MIN_DP" +using namespace std; + +// Help function for module + +void UnifyVcfHelp() +{ + printf ("\n"); + printf ("tvcutils %s-%s (%s) - Miscellaneous tools used by Torrent Variant Caller plugin and workflow.\n", + IonVersion::GetVersion().c_str(), IonVersion::GetRelease().c_str(), IonVersion::GetGitHash().c_str()); + printf ("\n"); + printf ("Usage: tvcutils unify_vcf [options]\n"); + printf ("\n"); + printf ("General options:\n"); + printf (" -t,--novel-tvc-vcf FILE small variants VCF file produced by TVC (required)\n"); + printf (" -i,--novel-assembly-vcf FILE long indels VCF file produced by TVCASSEMBLY (optional)\n"); + printf (" -a,--hotspot-annotation-vcf FILE hotspot annotations VCF file (optional)\n"); + printf (" -f,--reference-fasta FILE FASTA file containing reference genome (required)\n"); + printf (" -b,--target-file FILE target regions BED file (optional)\n"); + printf (" -o,--output-vcf FILE output merged and annotated VCF or VCF.GZ file (required)\n"); + printf (" -j,--tvc-metrics FILE JSON file with tvc metrics (optional)\n"); + printf (" -d,--input-depth FILE output of samtools depth. if provided, will cause generation of gvcf (optional, stdin ok)\n"); + printf (" -m,--min-depth INT minimum coverage depth in GVCF output (optional)\n"); + printf("\nVCF record filters:\n"); + printf(" --filter-by-target on/off Filter vcf records by meta information in the target bed file [on]\n"); + printf(" --hotspot-positions-only on/off Report only hotspot vcf records in final output [off]\n"); + printf(" --hotspot-variants-only on/off Suppress hotspot records that are no-calls or reference-calls [off]\n"); + printf("\nAllele Subset annotation:\n"); + printf(" --subset-check on/off Enables or disables subset allele subset annotation [on]\n"); + printf(" --subset-scores INT,INT,INT,INT Scores for Smith-Waterman aligner: match,mismatch,gap-open,gap-extend [1,-3,-5,-2]\n"); + printf(" --subset-simple-mnp on/off Simplified (faster) subset check for MNPs. [on]\n"); + printf ("\n"); +} + +// --------------------------------------------------------------------------------------- + void build_index(const string &path_in) { char buffer[33]; int return_code = 0; @@ -102,35 +138,7 @@ void build_index(const string &path_in) { if (tab == -1) {cerr << "build_index failed on tabix. " << return_code << endl;} } -using namespace std; - - - -void UnifyVcfHelp() -{ - printf ("\n"); - printf ("tvcutils %s-%s (%s) - Miscellaneous tools used by Torrent Variant Caller plugin and workflow.\n", - IonVersion::GetVersion().c_str(), IonVersion::GetRelease().c_str(), IonVersion::GetGitHash().c_str()); - printf ("\n"); - printf ("Usage: tvcutils unify_vcf [options]\n"); - printf ("\n"); - printf ("General options:\n"); - printf (" -t,--novel-tvc-vcf FILE small variants VCF file produced by TVC (required)\n"); - printf (" -i,--novel-assembly-vcf FILE long indels VCF file produced by TVCASSEMBLY (optional)\n"); - printf (" -a,--hotspot-annotation-vcf FILE hotspot annotations VCF file (optional)\n"); - printf (" -a,--hotspot-annotation-vcf FILE hotspot annotations VCF file (optional)\n"); - printf (" -f,--reference-fasta FILE FASTA file containing reference genome (required)\n"); - printf (" -b,--target-file FILE target regions BED file (optional)\n"); - printf (" -o,--output-vcf FILE output merged and annotated VCF or VCF.GZ file (required)\n"); - printf (" -j,--tvc-metrics FILE JSON file with tvc metrics (optional)\n"); - printf (" -d,--input-depth FILE output of samtools depth. if provided, will cause generation of gvcf (optional, stdin ok)\n"); - printf (" -m,--min-depth INT minimum coverage depth in GVCF output (optional)\n"); - printf ("\n"); -} - - - - +// --------------------------------------------------------------------------------------- CoverageInfoEntry* CoverageInfoEntry::parse(const char * line, const ReferenceReader& r) { CoverageInfoEntry* entry = new CoverageInfoEntry(r); @@ -180,7 +188,9 @@ int bgzf_stream::write_buffer() { return -1; } +// =========================================================================== // PriorityQueue methods implementations + bool PriorityQueue::get_next_variant(vcf::Variant* current) { // parse VCF line by line if (current->vcf->_done) return false; @@ -247,7 +257,47 @@ void PriorityQueue::left_align_variant(vcf::Variant* variant) { variant->alt[0] = alt; } +void PriorityQueue::open_vcf_file(vcf::VariantCallFile& vcf, string filename, bool parse_samples) { + // Open VCF file for parsing + if (!enabled) return; + vcf.parseSamples = parse_samples; + vcf.open(filename); + if (!vcf.is_open()) { + cerr << "ERROR: Could not open file : " << filename << " : " << strerror(errno) << endl; + exit(1); + } + vcf._done = false; + next(); +} + +void PriorityQueue::next() { + if (!enabled) return; + while (size() < _size) { + ComparableVcfVariant* v = new ComparableVcfVariant(merger, file, _vc++); + if (get_next_variant(v)) { + trim_variant(v); + left_align_variant(v); + push(v); + } else { + _size = 0; + delete_variant(v); + break; + } + } + delete_current(); + if (empty()) { + _current = NULL; + return; + } + _current = top(); + + pop(); +} + +// ===================================================================================== // VcfOrederedMerger methods implementations + + VcfOrderedMerger::VcfOrderedMerger(string& novel_tvc, string& assembly_tvc, string& hotspot_tvc, @@ -265,7 +315,13 @@ VcfOrderedMerger::VcfOrderedMerger(string& novel_tvc, assembly_queue(assembly_tvc, *this, r, w, la, true), hotspot_queue(hotspot_tvc, *this, r, w, la), bgz_out(output_tvc.c_str()), - current_cov_info(NULL) { + current_cov_info(NULL), + filter_by_target(true), + hotspot_positions_only(false), + hotspot_variants_only(false), + num_records(0), + num_filtered_records(0), + num_off_target(0) { if (!input_depth.empty()) { depth_in = (istream *)((input_depth == DEFAULT_STDIN_PARAM) ? &cin : new ifstream(input_depth.c_str())); gvcf_out = new ofstream(gvcf_output.c_str()); @@ -275,11 +331,21 @@ VcfOrderedMerger::VcfOrderedMerger(string& novel_tvc, current_target = targets_manager.merged.begin(); } + VcfOrderedMerger::~VcfOrderedMerger() { if (depth_in && depth_in != &cin) delete depth_in; if (gvcf_out) {gvcf_out->close(); delete gvcf_out;} } +void VcfOrderedMerger::SetVCFrecordFilters(bool filt_by_target, bool hotspot_pos_only, bool hotspot_var_only) +{ + filter_by_target = filt_by_target; + hotspot_positions_only = hotspot_pos_only; + hotspot_variants_only = hotspot_var_only; +} + +// ----------------------------------------------------------------------------------- + template int VcfOrderedMerger::variant_cmp(const T* v1, const vcf::Variant* v2) const { int idx_v1 = reference_reader.chr_idx(v1->sequenceName.c_str()); @@ -287,6 +353,22 @@ int VcfOrderedMerger::variant_cmp(const T* v1, const vcf::Variant* v2) const { return compare(idx_v1, v1->position, idx_v2, v2->position); } +// ----------------------------------------------------------------------------------- + +bool VcfOrderedMerger::too_far(vcf::Variant* v1, vcf::Variant* v2) { + if (v2 == NULL) return true; + int far = 50; + v2->position -= far; + int com = variant_cmp(v1, v2); + v2->position += far; // return to the original position + return (com == 1); +} + +// ----------------------------------------------------------------------------------- +// position in vcf is 1-based +// targets in bed format are 0-based open ended intervals +// with index conversion [0,x[ becomes ]0,x] + template bool VcfOrderedMerger::is_within_target_region(T *variant) { long pos = variant->position; @@ -300,10 +382,16 @@ bool VcfOrderedMerger::is_within_target_region(T *variant) { } return current_target != targets_manager.merged.end() && current_target->chr == chr_idx - && pos >= current_target->begin + && pos > current_target->begin && pos <= current_target->end; } + + + + +// ----------------------------------------------------------------------------------- + void VcfOrderedMerger::perform() { // merging ordered files loop while (novel_queue.has_value() && assembly_queue.has_value()) { @@ -328,17 +416,24 @@ void VcfOrderedMerger::perform() { assembly_queue.next(); } while (hotspot_queue.has_value()) { - blacklist_check(hotspot_queue.current()); + //blacklist_check(hotspot_queue.current()); + merge_annotation_into_vcf(hotspot_queue.current()); hotspot_queue.next(); } + flush_vcf(NULL); while (current_target != targets_manager.merged.end()) { gvcf_finish_region(); current_target++; } + cout << "VcfOrderedMerger: Wrote " << num_records << " vcf records, num_filtered_records= " + << num_filtered_records << " , num_off_target=" << num_off_target << endl; + allele_subset.print_stats(); } +// ----------------------------------------------------------------------------------- + void VcfOrderedMerger::write_header(vcf::VariantCallFile& vcf, string json_path) { - extend_header(vcf); + extend_header(vcf, allele_subset.check_enabled); parse_parameters_from_json(json_path, vcf); // write out the header to output bgz_out << vcf.header << "\n"; @@ -353,6 +448,8 @@ void VcfOrderedMerger::write_header(vcf::VariantCallFile& vcf, string json_path) } } +// ----------------------------------------------------------------------------------- + void VcfOrderedMerger::transfer_fields(map >& original_field, map >& new_field, map& field_types, @@ -388,6 +485,8 @@ void VcfOrderedMerger::transfer_fields(map >& original_fi } } +// ----------------------------------------------------------------------------------- + vcf::Variant* VcfOrderedMerger::merge_overlapping_variants() { vcf::Variant& novel_v = *novel_queue.current(); vcf::Variant& assembly_v = *assembly_queue.current(); @@ -431,6 +530,69 @@ vcf::Variant* VcfOrderedMerger::merge_overlapping_variants() { return assembly_queue.current(); } +// ----------------------------------------------------------------------------------- + +bool VcfOrderedMerger::find_match(vcf::Variant* merged_entry, string &hotspot_ref,vector::iterator oid, vector::iterator opos, vector::iterator oref, vector::iterator oalt, string *omapalt, int record_ref_extension, string &annotation_ref_extension) { + + if (merged_entry == NULL) return false; + string adj_omp; + if (record_ref_extension) { + if ((int)omapalt->length() < record_ref_extension || hotspot_ref.substr(hotspot_ref.length() - record_ref_extension) != + omapalt->substr(omapalt->length() - record_ref_extension)) { + return false; + cout << UNIFY_VARIANTS << " Hotspot annotation " << merged_entry->sequenceName + << ":" << merged_entry->position << ", allele " << *omapalt << " not eligible for shortening.\n"; + } + adj_omp = omapalt->substr(0, omapalt->length() - record_ref_extension); + } else adj_omp = *omapalt; + //*omapalt = *omapalt + annotation_ref_extension; + adj_omp = adj_omp+annotation_ref_extension; + + vector::iterator omapalti = + find(merged_entry->info["OMAPALT"].begin(), merged_entry->info["OMAPALT"].end(), adj_omp); + + if (omapalti == merged_entry->info["OMAPALT"].end()) { + return false; + // match older records. + cout << UNIFY_VARIANTS << " Hotspot annotation " << merged_entry->sequenceName + << ":" << merged_entry->position << ", allele " << *omapalt << " not found in merged variant file.\n"; + } + if (oref->length() >= 1 && oalt->length() >= 1 && (*oref)[0] == (*oalt)[0]) { + *oref = oref->substr(1); + *oalt = oalt->substr(1); + long p = atol(opos->c_str()); + stringstream ss; + ss<<++p; + *opos = ss.str(); + } + if (oref->empty()) { + *oref = "-"; + } + if(oalt->empty()) { + *oalt = "-"; + } + long idx = omapalti - merged_entry->info["OMAPALT"].begin(); + + if (merged_entry->info["OID"][idx] == ".") { + merged_entry->info["OID"][idx] = *oid; + merged_entry->info["OPOS"][idx] = *opos; + merged_entry->info["OREF"][idx] = *oref; + merged_entry->info["OALT"][idx] = *oalt; + merged_entry->info["OMAPALT"][idx] = /**omapalt*/ adj_omp; + } else { + merged_entry->info["OID"].push_back(*oid); + merged_entry->info["OPOS"].push_back(*opos); + merged_entry->info["OREF"].push_back(*oref); + merged_entry->info["OALT"].push_back(*oalt); + merged_entry->info["OMAPALT"].push_back(adj_omp /**omapalt*/); + } + if (merged_entry->id.size() == 0 or merged_entry->id == ".") merged_entry->id = *oid; + else merged_entry->id += ";"+ *oid; + return true; +} + +// ----------------------------------------------------------------------------------- + void VcfOrderedMerger::span_ref_and_alts() { long extension_length = novel_queue.current()->ref.length() - assembly_queue.current()->ref.length(); // extend alt sequences (span to the same length everywhere) @@ -455,6 +617,8 @@ void VcfOrderedMerger::span_ref_and_alts() { } } +// ----------------------------------------------------------------------------------- + void VcfOrderedMerger::generate_novel_annotations(vcf::Variant* variant) { if (variant->alt.empty()) return; for (vector::iterator alt = variant->alt.begin(); alt != variant->alt.end(); alt++) { @@ -462,7 +626,6 @@ void VcfOrderedMerger::generate_novel_annotations(vcf::Variant* variant) { long opos = variant->position; string oref = variant->ref; string temp_alt = *alt; - // trim identical ends string::iterator orefi = oref.end(); string::iterator alti = temp_alt.end(); @@ -470,16 +633,14 @@ void VcfOrderedMerger::generate_novel_annotations(vcf::Variant* variant) { if (alti + 1 < temp_alt.end()) temp_alt.erase(alti + 1, temp_alt.end()); if (orefi + 1 < oref.end()) oref.erase(orefi + 1, oref.end()); - // trim identical beginnings orefi = oref.begin(); alti = temp_alt.begin(); - for (; *orefi == *alti && *alti != '-'; orefi++, alti++) { + for (; *orefi == *alti && *alti != '-' and alti < temp_alt.end() and orefi < oref.end(); orefi++, alti++) { opos++; } if (distance(temp_alt.begin(), alti) > 0) temp_alt.erase(temp_alt.begin(), alti); if (distance(oref.begin(), orefi) > 0) oref.erase(oref.begin(), orefi); - if (oref.empty()) oref = "-"; if (temp_alt.empty()) temp_alt = "-"; stringstream ss; @@ -490,18 +651,81 @@ void VcfOrderedMerger::generate_novel_annotations(vcf::Variant* variant) { push_value_to_vector(variant->info["OREF"], i, oref); push_value_to_vector(variant->info["OALT"], i, temp_alt); push_value_to_vector(variant->info["OMAPALT"], i, *alt); + // We only write a subset info field if there actually are subsets } } + +// ----------------------------------------------------------------------------------- +// implementation of subset check and annotation XXX + +void VcfOrderedMerger::annotate_subset(vcf::Variant* variant) { + + if (not allele_subset.check_enabled) + return; + // Immediately return if this is a NOCALL or not a multi allele variant + if (variant->filter != "PASS" or variant->alt.size()<2) + return; + + // unpack called alleles + vector called_alts; + string gt_field; + stringstream gt(variant->samples[variant->sampleNames[0]]["GT"][0]); + while (getline(gt, gt_field, '/')){ + if (gt_field!="." and gt_field!="0"){ + int called_allele = std::stoi(gt_field)-1; // Zero based index for vector access + // Have we seen this one before? + unsigned int idx = 0; + while (idx subset_info; + bool have_subsets = false; + + // For every alt allele we test it is a strict subset of any called alt alleles + for (unsigned int aidx=0; aidxalt.size(); ++aidx){ + string si_field; + for (vector::iterator calt=called_alts.begin(); calt!=called_alts.end(); ++calt){ + if ((int)aidx == *calt) + continue; + if (allele_subset.is_allele_subset(variant->ref, variant->alt[aidx], variant->alt[*calt])){ + have_subsets = true; + if (not si_field.empty()) + si_field += "/"; + si_field += std::to_string(*calt+1); // In vcf, make it 1-based again + } + } + if (si_field.empty()) + push_value_to_vector(subset_info, aidx, "."); + else + push_value_to_vector(subset_info, aidx, si_field); + } + + // And if we found subsets we annotate them in the info field + if (have_subsets) + variant->info["SUBSET"] = subset_info; +} + +// ----------------------------------------------------------------------------------- + void VcfOrderedMerger::merge_annotation_into_vcf(vcf::Variant* merged_entry, vcf::Variant* hotspot) { string annotation_ref_extension; long record_ref_extension = 0; - if (hotspot->ref.length() > merged_entry->ref.length()) { - record_ref_extension = hotspot->ref.length() - merged_entry->ref.length(); - } - if (hotspot->ref.length() < merged_entry->ref.length()) { - annotation_ref_extension = merged_entry->ref.substr(hotspot->ref.length()); + if (merged_entry) { + if (hotspot->ref.length() > merged_entry->ref.length()) { + record_ref_extension = hotspot->ref.length() - merged_entry->ref.length(); + } + if (hotspot->ref.length() < merged_entry->ref.length()) { + annotation_ref_extension = merged_entry->ref.substr(hotspot->ref.length()); + } } // Form blacklist @@ -513,7 +737,7 @@ void VcfOrderedMerger::merge_annotation_into_vcf(vcf::Variant* merged_entry, vcf blacklist[*key] = *v; } - vector filtered_oid; + //vector filtered_oid; for (vector::iterator oid = hotspot->info["OID"].begin(), opos = hotspot->info["OPOS"].begin(), @@ -525,74 +749,129 @@ void VcfOrderedMerger::merge_annotation_into_vcf(vcf::Variant* merged_entry, vcf oid++, opos++, oref++, oalt++, omapalt++) { if (!blacklist.empty() && blacklist[*omapalt] != ".") continue; - filtered_oid.push_back(*oid); - - if (record_ref_extension) { - if ((int)omapalt->length() < record_ref_extension || hotspot->ref.substr(hotspot->ref.length() - record_ref_extension) != - omapalt->substr(omapalt->length() - record_ref_extension)) { - cout << UNIFY_VARIANTS << " Hotspot annotation " << merged_entry->sequenceName - << ":" << merged_entry->position << ", allele " << *omapalt << " not eligible for shortening.\n"; - continue; - } - *omapalt = omapalt->substr(0, omapalt->length() - record_ref_extension); + //if (merged_entry) filtered_oid.push_back(*oid); + //ZZ + if (not find_match(merged_entry, hotspot->ref,oid, opos, oref, oalt, &(*omapalt), record_ref_extension, annotation_ref_extension)) { + list::reverse_iterator it; + bool found = false; + for (it = variant_list.rbegin(); it != variant_list.rend(); it++ ) { + if (too_far(&(*it), hotspot)) break; + int padding = hotspot->position-it->position; + if (padding > (int) it->ref.length()) continue; // not contain + long record_ref_ext = 0; // new + string annotation_ref_ext; // new + string x = it->ref.substr(0,padding)+*omapalt; + unsigned int rlen = padding + hotspot->ref.length(); + string hotspot_ref = it->ref.substr(0,padding)+hotspot->ref; + if (rlen > it->ref.length()) { + record_ref_ext = rlen - it->ref.length(); + } + if (rlen < it->ref.length()) { + annotation_ref_ext = it->ref.substr(rlen); + } + if (find_match(&(*it), hotspot_ref, oid, opos, oref, oalt, &x, record_ref_ext, annotation_ref_ext)) {found = true; break;} + } + if (not found){ + cout << UNIFY_VARIANTS << " Hotspot annotation " << hotspot->sequenceName + << ":" << hotspot->position << ", allele " << *omapalt << " not found in merged variant file.\n"; + } } - *omapalt = *omapalt + annotation_ref_extension; + } +} - vector::iterator omapalti = - find(merged_entry->info["OMAPALT"].begin(), merged_entry->info["OMAPALT"].end(), *omapalt); +// ----------------------------------------------------------------------------------- - if (omapalti == merged_entry->info["OMAPALT"].end()) { - cout << UNIFY_VARIANTS << " Hotspot annotation " << merged_entry->sequenceName - << ":" << merged_entry->position << ", allele " << *omapalt << " not found in merged variant file.\n"; - continue; - } - if (oref->length() >= 1 && oalt->length() >= 1 && (*oref)[0] == (*oalt)[0]) { - *oref = oref->substr(1); - *oalt = oalt->substr(1); - long p = atol(opos->c_str()); - stringstream ss; - ss<<++p; - *opos = ss.str(); - } - if (oref->empty()) { - *oref = "-"; - } - if(oalt->empty()) { - *oalt = "-"; - } - long idx = omapalti - merged_entry->info["OMAPALT"].begin(); +bool VcfOrderedMerger::filter_VCF_record(vcf::Variant* record) const +{ + bool is_HS = record->infoFlags.find("HS") != record->infoFlags.end(); + + // Hotspot positions only filter applied first + if (hotspot_positions_only and not is_HS) + return true; + + // Next we filter by target meta data + if (filter_by_target){ + // vcf->position is 1-based, whereas target bed fiels are 0-based indices. + int hs_only = targets_manager.ReportHotspotsOnly(*current_target, reference_reader.chr_idx(record->sequenceName.c_str()), record->position-1); + if (hs_only > 0 and not is_HS) + return true; + } - if (merged_entry->info["OID"][idx] == ".") { - merged_entry->info["OID"][idx] = *oid; - merged_entry->info["OPOS"][idx] = *opos; - merged_entry->info["OREF"][idx] = *oref; - merged_entry->info["OALT"][idx] = *oalt; - merged_entry->info["OMAPALT"][idx] = *omapalt; - } else { - merged_entry->info["OID"].push_back(*oid); - merged_entry->info["OPOS"].push_back(*opos); - merged_entry->info["OREF"].push_back(*oref); - merged_entry->info["OALT"].push_back(*oalt); - merged_entry->info["OMAPALT"].push_back(*omapalt); + // Finally filter hotspot lines that are REF of NOCALL + if (hotspot_variants_only and is_HS){ + // 1) Remove NOCALLs + if (record->filter != "PASS") + return true; + + // 2) Remove reference calls + bool ref_call = true; + string gt_field; + for (vector::const_iterator its = record->sampleNames.begin(); its != record->sampleNames.end(); ++its) { + if (not ref_call) + break; + map > & sampleOutput = record->samples[*its]; + map >::const_iterator itg = sampleOutput.find("GT"); + if (itg == sampleOutput.end()) + return false; + + for (vector::const_iterator itv = itg->second.begin(); itv!=itg->second.end(); ++itv){ + stringstream ss(*itv); + while (getline(ss, gt_field, '/')){ + if (gt_field == "0" or gt_field == ".") + continue; + else + ref_call = false; + } + } } + return ref_call; } - if (!filtered_oid.empty()) { - merged_entry->id = join(filtered_oid, ";"); + + return false; +} + +// ----------------------------------------------------------------------------------- +// XXX Code below writes lines to the vcf files + +void VcfOrderedMerger::flush_vcf(vcf::Variant* latest) +{ + while (not variant_list.empty()) { + vcf::Variant* current = &(*variant_list.begin()); + + if (too_far(current, latest)) { + if (is_within_target_region(current)) { + if (filter_VCF_record(current)) + ++num_filtered_records; + else { // Write out record if not filtered + gvcf_process(current->sequenceName.c_str(), current->position); + gvcf_out_variant(current); + bgz_out << *current << "\n"; + ++num_records; + } + } else { + ++num_off_target; + cout << UNIFY_VARIANTS " Skipping " << current->sequenceName << ":" << current->position + << " outside of target regions.\n"; + } + variant_list.pop_front(); + } else break; } + if (latest) + variant_list.push_back(*latest); } +// ----------------------------------------------------------------------------------- + void VcfOrderedMerger::process_and_write_vcf_entry(vcf::Variant* current) { generate_novel_annotations(current); - process_annotation(current); - if (is_within_target_region(current)) { - gvcf_process(current->sequenceName.c_str(), current->position); - gvcf_out_variant(current); - bgz_out << *current << "\n"; - - } else cout << UNIFY_VARIANTS " Skipping " << current->sequenceName << ":" << current->position - << " outside of target regions.\n"; + process_annotation(current); // Adds hotspot annotation to entry + annotate_subset(current); // Checks if called alleles supersets of others + flush_vcf(current); // Filters entries and writes vcf files to file + return; } +// ----------------------------------------------------------------------------------- + void VcfOrderedMerger::gvcf_out_variant(vcf::Variant *current) { if (gvcf_out) { while (current_cov_info && current_cov_info->position - current->position < (int)current->ref.length()) next_cov_entry(); @@ -600,6 +879,9 @@ void VcfOrderedMerger::gvcf_out_variant(vcf::Variant *current) { } } +// ----------------------------------------------------------------------------------- +// Generates coverage lines in gvcf file + void VcfOrderedMerger::gvcf_process(int chr, long position) { if (!gvcf_out || !current_cov_info) return; @@ -637,6 +919,8 @@ void VcfOrderedMerger::gvcf_process(int chr, long position) { } } +// ----------------------------------------------------------------------------------- + void VcfOrderedMerger::gvcf_finish_region() { gvcf_process(current_target->chr, current_target->end + 1); } @@ -645,6 +929,8 @@ void VcfOrderedMerger::gvcf_process(const char * seq_name, long pos) { gvcf_process(reference_reader.chr_idx(seq_name), pos); } +// ----------------------------------------------------------------------------------- + vcf::Variant VcfOrderedMerger::generate_gvcf_entry(vcf::VariantCallFile& current, int chr, depth_info depth, long pos, long end) const { stringstream ss; @@ -676,6 +962,8 @@ vcf::Variant VcfOrderedMerger::generate_gvcf_entry(vcf::VariantCallFile& current return gvcf_entry; } +// ----------------------------------------------------------------------------------- + void VcfOrderedMerger::next_cov_entry() { if (current_cov_info != NULL) delete current_cov_info; if (depth_in->eof()) { @@ -691,33 +979,28 @@ void VcfOrderedMerger::next_cov_entry() { current_cov_info = CoverageInfoEntry::parse(line, reference_reader); } +// ----------------------------------------------------------------------------------- + void VcfOrderedMerger::process_annotation(vcf::Variant* current) { + int cmp; - if (hotspots_.size() > 0) { - for (vector::iterator iter = hotspots_.begin(); (iter != hotspots_.end()); ++iter) { - cmp = variant_cmp(current, &(*iter)); - if (cmp == 1) break; + if (hotspot_queue.has_value()) + do { + cmp = variant_cmp(current, hotspot_queue.current()); + if (cmp == 1) { /*hotspots_.push_back(*hotspot_queue.current());*/ return;} if (cmp == 0) { - merge_annotation_into_vcf(current, &(*iter)); - break; + merge_annotation_into_vcf(current, hotspot_queue.current()); + hotspot_queue.next(); + return; } - //blacklist_check(&(*iter)); - } - } - if (hotspots_.size() > 100) {hotspots_.erase(hotspots_.begin());} - if (hotspot_queue.has_value()) do { - cmp = variant_cmp(current, hotspot_queue.current()); - hotspots_.push_back(*hotspot_queue.current()); - if (cmp == 1) return; - if (cmp == 0) { - merge_annotation_into_vcf(current, hotspot_queue.current()); + merge_annotation_into_vcf(hotspot_queue.current()) ; + //blacklist_check(hotspot_queue.current()); // does not need, it is checked in above hotspot_queue.next(); - return; } - blacklist_check(hotspot_queue.current()); - hotspot_queue.next(); - } while (cmp == -1 && hotspot_queue.has_value()); + while (cmp == -1 && hotspot_queue.has_value()); } + +// ----------------------------------------------------------------------------------- void VcfOrderedMerger::blacklist_check(vcf::Variant* hotspot) { map >::iterator bstr = hotspot->info.find("BSTRAND"); @@ -732,52 +1015,27 @@ void VcfOrderedMerger::blacklist_check(vcf::Variant* hotspot) { << hotspot->position << " not found in merged variant file.\n"; } -void PriorityQueue::open_vcf_file(vcf::VariantCallFile& vcf, string filename, bool parse_samples) { - // Open VCF file for parsing - if (!enabled) return; - vcf.parseSamples = parse_samples; - vcf.open(filename); - if (!vcf.is_open()) { - cerr << "ERROR: Could not open file : " << filename << " : " << strerror(errno) << endl; - exit(1); - } - vcf._done = false; - next(); -} - -void PriorityQueue::next() { - if (!enabled) return; - while (size() < _size) { - ComparableVcfVariant* v = new ComparableVcfVariant(merger, file, _vc++); - if (get_next_variant(v)) { - trim_variant(v); - left_align_variant(v); - push(v); - } else { - _size = 0; - delete_variant(v); - break; - } - } - delete_current(); - if (empty()) { - _current = NULL; - return; - } - _current = top(); - - pop(); -} - +// ================================================================================ // ComparableVcfVariant wrapper implementation + bool ComparableVcfVariant::operator<(ComparableVcfVariant& b) { int r = merger.variant_cmp(this, &b); // StableSorting - if (r == 0) { r = -comp(_t, b._t); } + if (r == 0) { + //ZZ favor HS tag + int x = 1, y= 1; + if (this->infoFlags.find("HS") == this->infoFlags.end()) x = 0; + if (b.infoFlags.find("HS")== b.infoFlags.end()) y = 0; + if (x == y) r = -comp(_t, b._t); + else if (x < y) r = -1; + else r = 1; + } return r == -1; } +// ================================================================================ // Common procedures (functions) + void push_value_to_vector(vector& v, long index, const string& entry) { if (index < 0 || (unsigned)index >= v.size()) v.push_back(entry); @@ -804,7 +1062,7 @@ int compare(const T1& p11, const T2& p12, const T1& p21, const T2& p22) { return compare(make_pair(p11, p12), make_pair(p21, p22)); } -void extend_header(vcf::VariantCallFile &vcf) { +void extend_header(vcf::VariantCallFile &vcf, bool add_subset) { // extend header with new info fields vcf.addHeaderLine("##INFO="); vcf.addHeaderLine("##INFO="); @@ -812,6 +1070,9 @@ void extend_header(vcf::VariantCallFile &vcf) { vcf.addHeaderLine("##INFO="); vcf.addHeaderLine("##INFO="); + if (add_subset) + vcf.addHeaderLine("##INFO="); } void parse_parameters_from_json(string filename, vcf::VariantCallFile& vcf) { @@ -876,6 +1137,9 @@ bool check_on_write(string filename, string param_name) { return true; } +// ============================================================================== +// XXX Main function + int UnifyVcf(int argc, const char *argv[]) { unsigned int DEFAULT_WINDOW_SIZE = 10; int DEFAULT_MIN_DP = 0; @@ -904,8 +1168,19 @@ int UnifyVcf(int argc, const char *argv[]) { string input_depth_param = "input-depth"; string input_depth = opts.GetFirstString('d', input_depth_param, ""); - int min_depth = opts.GetFirstInt('m', "min-depth", 0); - int window_size = opts.GetFirstInt('w', "window-size", DEFAULT_WINDOW_SIZE); + int min_depth = opts.GetFirstInt('m', "min-depth", 0); + int window_size = opts.GetFirstInt('w', "window-size", DEFAULT_WINDOW_SIZE); + + // VCF record filter settings + bool filter_by_target = opts.GetFirstBoolean ('-', "filter-by-target", true); + bool hotspot_positions_only = opts.GetFirstBoolean ('-', "hotspot-positions-only", false); + bool hotspot_variants_only = opts.GetFirstBoolean ('-', "hotspot-variants-only", false); + + // Subset annotation options + bool subset_debug = opts.GetFirstBoolean ('-', "subset-debug", false); + bool check_for_subsets = opts.GetFirstBoolean ('-', "subset-check", true); + bool subset_simple_mnp = opts.GetFirstBoolean ('-', "subset-simple-mnp", true); + vector subset_scores = opts.GetFirstIntVector('-', "subset-scores", "1,-3,-5,-2"); // check and fill optional arguments unsigned int w = (window_size < 1) ? DEFAULT_WINDOW_SIZE : (unsigned int) window_size; @@ -950,12 +1225,389 @@ int UnifyVcf(int argc, const char *argv[]) { // Prepare merger object VcfOrderedMerger merger(novel_vcf, assembly_vcf, hotspot_vcf, output_vcf, json_path, input_depth, output_gvcf, reference_reader, targets_namager, w, minimum_depth,DEFAULT_LEFT_ALIGN_FLAG); + // Set filtering options + merger.SetVCFrecordFilters(filter_by_target, hotspot_positions_only, hotspot_variants_only); + // Set subset annotation options + merger.allele_subset.debug = subset_debug; + merger.allele_subset.check_enabled = check_for_subsets; + merger.allele_subset.simple_mnp_alignment = subset_simple_mnp; + merger.allele_subset.SetAlignerScores(subset_scores); // Perform merging procedure merger.perform(); } // build tabix indices build_index(output_vcf); - if (!output_gvcf.empty()) build_index(output_gvcf); + if (!output_gvcf.empty()) + build_index(output_gvcf); return 0; } + +// ============================================================================== +// class AlleleSubsetCheck contains functionality to identify alleles that are strict +// subsets of other alleles +// The method to search for a subset is a two stage conditional multiple sequence alignment +// It's suboptimal compared to a full 3D multiple sequence alignment, but faster. +// TODO: explore vcflib Smith-Waterman instead of Realigner + +AlleleSubsetCheck::AlleleSubsetCheck() : + aligner_(50,10),check_enabled(true), simple_mnp_alignment(true) +{ + // Default to tmap default scores for alignment + vector def_scores(4, 1); + def_scores[1] = -3; // mismatch penalty + def_scores[2] = -5; // gap open penalty + def_scores[3] = -2; // gap extend penalty + aligner_.SetScores(def_scores); + + // We do not allow any clipping and we only align in forward direction + aligner_.SetClipping(0, true); + + reset_counters(); + failure_ = false; + debug = false; +} + +// -------------------------------------------------------------------- +// A fast simplified subset check for MNPs that only allows matches and mismatches as alignment operations +// Otherwise same conditional 2-stage alignment approach as in conditional_alignment_subset_check() +bool AlleleSubsetCheck::mnp_subset_check(const string & ref, const string &subset, const string &super) +{ + int n_edits_ref = 0; + int n_edits_sup = 0; + bool rmatch, smatch; + + for (unsigned int i=0; i< subset.length(); ++i){ + rmatch = ref[i]==subset[i]; + smatch = subset[i]!=super[i]; + + if (not rmatch) { + ++n_edits_ref; + if (not smatch) + return false; + } + else if (not smatch) + ++n_edits_sup; + } + return (n_edits_ref>0 and n_edits_sup>0); +} + +// -------------------------------------------------------------------- +// Check for M cigar operations which can be matches or mismatches + +bool AlleleSubsetCheck::is_match(const string & pretty_a, int aidx) +{ + if (aidx>=0 and aidx<(int)pretty_a.length()){ + return (pretty_a[aidx]=='|' or pretty_a[aidx]==' '); + } + else { + return true; // To get boundary conditions at edges right + } +} + +bool AlleleSubsetCheck::have_matches(const string & pretty_1, int idx1, const string & pretty_2, int idx2) +{ + return (is_match(pretty_1, idx1) and is_match(pretty_2, idx2)); +} + +// -------------------------------------------------------------------- +// A fast MNP alignment that only allows matches and mismatches as operations + +string AlleleSubsetCheck::get_mnp_pretty_align(const string & allele1, const string & allele2) +{ + if (allele1.length() != allele2.length()) + return ""; + string pretty_a(allele1.length(), '|'); + for (unsigned int i=0; i ref - subset alignment + + unsigned int start_position_shift; + string pretty_ref; + if (simple_mnp_alignment and ref.length()==subset.length()){ + pretty_ref = get_mnp_pretty_align(ref, subset); + } + else{ + aligner_.SetSequences(subset, ref, pretty_ref ,true); + aligner_.computeSWalignment(cigar_data, md_data, start_position_shift); + pretty_ref = aligner_.pretty_aln(); + } + + // Second stage, get subset - superset alignment + + string pretty_sup; + if (simple_mnp_alignment and subset.length()==superset.length()){ + pretty_sup = get_mnp_pretty_align(subset, superset); + } + else{ + aligner_.SetSequences(superset, subset, pretty_sup ,true); + aligner_.computeSWalignment(cigar_data, md_data, start_position_shift); + pretty_sup = aligner_.pretty_aln(); + } + + // Reconcile alignments to get conditional superset alignment + string cond_super_aln = get_cond_superset_alignment(pretty_ref, pretty_sup); + + // Debug break point here: XXX + if (debug and (failure_ or (cond_super_aln.length() > 0))) { + if (failure_) + cout << "FAILURE for Ref: " << ref << " \"" << pretty_ref << "\" Subset: " << subset << " \"" << pretty_sup << "\" Superset: " << superset << endl; + else + cout << "SUPERSET for Ref: " << ref << " \"" << pretty_ref << "\" Subset: " << subset << " \"" << pretty_sup << "\" Superset: " << superset << endl; + cout << "Conditional alignment: Ref: " << ref << " Superset: " << superset << " Pretty: \"" << cond_super_aln << "\"" << endl << endl; + //string dinput; + //getline(cin, dinput); + //cout << dinput << endl; + failure_ = false; + } + //*/ + + + if (cond_super_aln.length() > 0) + return true; + else + return false; +} + +// -------------------------------------------------------------------- +// Function reconciles the two alignment stages and creates a conditional +// ref<->superset alignment if possible. Otherwise returns an empty string. +// +// With events match='|' mismatch=' ' M=' 'or'|' insertion='+' deletion='-', +// to have a valid subset decomposition with independent, disjoint events +// if we only allow the following operations: +// (extension of a gap is NOT a superset of the shorter gap) +// +// Ref:t <-> q:Sub:t <-> q:Super +// | M +// ' ' | +// M|||M M---M +// M---M flanking M +// flanking M M+++M +// M+++M M|||M + + +string AlleleSubsetCheck::get_cond_superset_alignment(const string & pretty_ref, const string &pretty_super) +{ + string super_aln; + int ridx = 0; + int sidx = 0; + + // Iterate over the different alignment operations; one event per loop execution + while (ridx<(int)pretty_ref.length() or sidx<(int)pretty_super.length()) { + bool new_event = true; + + // Events that increment both ridx and sidx + if (ridx<(int)pretty_ref.length() and sidx<(int)pretty_super.length()){ + + // 1) Invalid subset operation + if (pretty_ref[ridx]==' ' and pretty_super[sidx]==' '){ + return ""; + } + + // 2) match event + if (pretty_ref[ridx]=='|' and pretty_super[sidx] =='|'){ + super_aln += '|'; + ++ridx; ++sidx; + continue; + } + + // 3) mismatches event + if (have_matches(pretty_ref, ridx, pretty_super, sidx)){ + super_aln += ' '; + ++ridx; ++sidx; + continue; + } + + // 4) Deletion in t:Sub <-> q:Super + while (ridx<(int)pretty_ref.length() and sidx<(int)pretty_super.length() and pretty_super[sidx] =='-'){ + if (pretty_ref[ridx] != '|') + return ""; + // Enforce left M operation + if (new_event){ + new_event = false; + if (not have_matches(pretty_ref, ridx-1, pretty_super, sidx-1)) + return ""; + } + super_aln += '-'; + ++ridx; ++sidx; + } + // Enforce trailing M operation if event was triggered + if (not new_event){ + if (not have_matches(pretty_ref, ridx, pretty_super, sidx)) + return ""; + continue; + } + + // 5) Insertion in t:Ref <-> q:Sub + while (ridx<(int)pretty_ref.length() and sidx<(int)pretty_super.length() and pretty_ref[ridx] =='+'){ + if (pretty_super[sidx] != '|') + return ""; + // Enforce left M operation + if (new_event){ + new_event = false; + if (not have_matches(pretty_ref, ridx-1, pretty_super, sidx-1)) + return ""; + } + super_aln += '+'; + ++ridx; ++sidx; + } + // Enforce trailing M operation if event was triggered + if (not new_event){ + if (not have_matches(pretty_ref, ridx, pretty_super, sidx)) + return ""; + continue; + } + + } // End double incrementing events + + // 6) Deletion in t:Ref <-> q:Sub + if (ridx<(int)pretty_ref.length() and pretty_ref[ridx] =='-'){ + // Need flanking M cigar operations + if (not (have_matches(pretty_ref, ridx-1, pretty_super, sidx-1) and is_match(pretty_super, sidx))) + return ""; + while (ridx<(int)pretty_ref.length() and pretty_ref[ridx] =='-'){ + super_aln += '-'; + ++ridx; + } + if (not is_match(pretty_ref, ridx)) + return ""; + continue; + } + + // 7) Insertion in t:Sub <-> q:Super + if (sidx<(int)pretty_super.length() and pretty_super[sidx] =='+'){ + // Need flanking M cigar operations + if (not (have_matches(pretty_ref, ridx-1, pretty_super, sidx-1)and is_match(pretty_ref, ridx))) + return ""; + while (sidx<(int)pretty_super.length() and pretty_super[sidx] =='+'){ + super_aln += '+'; + ++sidx; + } + if (not is_match(pretty_super, sidx)) + return ""; + continue; + } + + // 8) If we are at this point it means we went through the whole loop + // without triggering an event - Fail and report + //cerr << "WARNING AlleleSubsetCheck::get_cond_superset_alignment failed to trigger event for " + // << "\"" << pretty_ref << "\":" << ridx << " \"" << pretty_super << "\":" << sidx + // << " super_aln=" << super_aln << endl; + ++counter_failures; + failure_ = true; + return ""; + } + + return super_aln; +} + +// -------------------------------------------------------------------- + +void AlleleSubsetCheck::reset_counters() +{ + counter_num_align_checks = 0; + counter_num_align_subsets = 0; + counter_num_mnp_checks = 0; + counter_num_mnp_subsets = 0; + counter_failures = 0; +} + +// -------------------------------------------------------------------- +void AlleleSubsetCheck::print_stats() +{ + if (not check_enabled){ + cout << "Allele Subset Annotation Summary: Disabled." << endl; + return; + } + + ostringstream table; + table << endl << "Allele Subset Annotation Summary:" << endl; + table << setw(23) << " "; + table << setw(23) << "--------------------" << setw(23) << "--------------------" << endl; + table << setw(23) << " "; + table << setw(23) << "Pairs Investigated" << setw(23) << "Subsets found" << endl; + table << setw(23) << " "; + table << setw(23) << "--------------------" << setw(23) << "--------------------" << endl; + table << setw(23) << "MNP alignment"; + table << setw(23) << counter_num_mnp_checks << setw(23) << counter_num_mnp_subsets << endl; + table << setw(23) << "Smith-Waterman"; + table << setw(23) << counter_num_align_checks << setw(23) << counter_num_align_subsets << endl; + + table << setw(23) << " "; + table << setw(23) << "--------------------" << setw(23) << "--------------------" << endl; + table << setw(23) << "Total"; + table << setw(23) << (counter_num_mnp_checks+counter_num_align_checks) + << setw(23) << (counter_num_mnp_subsets+counter_num_align_subsets) << endl; + if (counter_failures>0) + table << "Number of pair failures: " << counter_failures << endl; + cout << table.str() << endl; +} + diff --git a/Analysis/VariantCaller/tvcutils/unify_vcf.h b/Analysis/VariantCaller/tvcutils/unify_vcf.h index 9a01c642..f165a335 100644 --- a/Analysis/VariantCaller/tvcutils/unify_vcf.h +++ b/Analysis/VariantCaller/tvcutils/unify_vcf.h @@ -5,6 +5,8 @@ #ifndef ION_ANALYSIS_UNIFY_VCF_H #define ION_ANALYSIS_UNIFY_VCF_H +#include "Realigner.h" + class bgzf_stream; class VcfOrderedMerger; class PriorityQueue; @@ -12,6 +14,57 @@ class ComparableVcfVariant; void build_index(const string &path_to_gz); +// --------------------------------------------------------------------------------------- + +class AlleleSubsetCheck +{ +private: + Realigner aligner_; + + // dummy variables for aligner + vector cigar_data; + vector md_data; + + // accounting variables + unsigned int counter_num_align_checks; + unsigned int counter_num_align_subsets; + unsigned int counter_num_mnp_checks; + unsigned int counter_num_mnp_subsets; + unsigned int counter_failures; + bool failure_; + + bool mnp_subset_check(const string & ref, const string &subset, const string &super); + bool conditional_alignment_subset_check(const string & ref, const string &subset, const string &super); + bool is_match(const string & pretty_a, int aidx); + bool have_matches(const string & pretty_a, int aidx, const string & pretty_b, int bidx); + bool anchor_sanity_check(const string & ref, const string &subset, const string &super); + string get_mnp_pretty_align(const string & allele1, const string & allele2); + string get_cond_superset_alignment(const string & pretty_ref, const string &pretty_super); + +public: + AlleleSubsetCheck(); + + // The main function to check if a subset allele hypothesis is in fact a subset of a superset allele + bool is_allele_subset(const string & ref, const string &subset, const string &super); + + // Adjusts Smith-Waterman penalties - match + void SetAlignerScores(const vector & scores) + { + aligner_.SetScores(scores); + } + + void reset_counters(); + void print_stats(); + + bool check_enabled; + bool simple_mnp_alignment; + bool debug; // If enabled shows information about failures as well as conditional subset alignments + +}; + + +// --------------------------------------------------------------------------------------- + struct CoverageInfoEntry { string sequenceName; long position; @@ -35,6 +88,8 @@ struct CoverageEntryComparator { bool operator()(const CoverageInfoEntry& lhs, const CoverageInfoEntry& rhs) { return lhs.cov < rhs.cov; } }; +// --------------------------------------------------------------------------------------- + class bgzf_stream { BGZF *bgzf; string data; @@ -55,6 +110,8 @@ class bgzf_stream { int write(int length); }; +// --------------------------------------------------------------------------------------- + class ComparableVcfVariant : public vcf::Variant { const VcfOrderedMerger& merger; unsigned long _t; @@ -65,12 +122,16 @@ class ComparableVcfVariant : public vcf::Variant { bool operator<(ComparableVcfVariant& b); }; +// --------------------------------------------------------------------------------------- + class VariantComparator { public: VariantComparator() {} bool operator()(ComparableVcfVariant* lhs, ComparableVcfVariant* rhs) { return (*lhs)<(*rhs); } }; +// --------------------------------------------------------------------------------------- + typedef priority_queue, VariantComparator> variant_queue; class PriorityQueue : private variant_queue { @@ -91,7 +152,10 @@ class PriorityQueue : private variant_queue { bool parse_samples = false) : variant_queue(VariantComparator()), _size(w), _vc(0), _current(NULL), left_align_enabled(la), enabled(!filename.empty()), - merger(merger), reference_reader(reader) { open_vcf_file(file, filename, parse_samples); } + merger(merger), reference_reader(reader) + { + open_vcf_file(file, filename, parse_samples); + } ~PriorityQueue() { while (_current) next(); } @@ -117,6 +181,8 @@ class PriorityQueue : private variant_queue { void delete_current() { delete_variant(_current); } }; +// --------------------------------------------------------------------------------------- + class VcfOrderedMerger { public: VcfOrderedMerger(string& novel_tvc, @@ -138,7 +204,11 @@ class VcfOrderedMerger { template bool is_within_target_region(T *variant); + void SetVCFrecordFilters(bool filt_by_target, bool hotspot_pos_only, bool hotspot_var_only); void perform(); + + AlleleSubsetCheck allele_subset; + private: istream* depth_in; @@ -148,20 +218,40 @@ class VcfOrderedMerger { bool left_align_enabled; size_t window_size, minimum_depth; PriorityQueue novel_queue, assembly_queue, hotspot_queue; - vector hotspots_; + list hotspots_; ofstream bgz_out; vector::iterator current_target; CoverageInfoEntry* current_cov_info; + // VCF record filters (applied during vcf merging) + bool filter_by_target; // Filter records based on mets information in target bed info field + bool hotspot_positions_only; // Output only vcf lines with the infoFlag 'HS' + bool hotspot_variants_only; // Suppress hotspot reference calls and no-calls from the final output vcf + + // Accounting variables + unsigned int num_records; + unsigned int num_filtered_records; + unsigned int num_off_target; + + // Function that determines if a vcf record should be filtered + bool filter_VCF_record(vcf::Variant* record) const; + void write_header(vcf::VariantCallFile& vcf, string json_path); vcf::Variant* merge_overlapping_variants(); + list variant_list; void span_ref_and_alts(); + bool too_far(vcf::Variant*, vcf::Variant*); void generate_novel_annotations(vcf::Variant* variant); void merge_annotation_into_vcf(vcf::Variant* merged_entry, vcf::Variant* hotspot); + void merge_annotation_into_vcf(vcf::Variant* hotspot) { merge_annotation_into_vcf(NULL, hotspot);}; + void flush_vcf(vcf::Variant* latest); + void annotate_subset(vcf::Variant* variant); + bool find_match(vcf::Variant* merged_entry, string &hotspot_ref,vector::iterator oid, vector::iterator opos, vector::iterator oref, vector::iterator oalt, string *omapalt, int record_ref_extension, string &annotation_ref_extension); + void process_and_write_vcf_entry(vcf::Variant* current); @@ -189,6 +279,8 @@ class VcfOrderedMerger { void gvcf_finish_region(); }; +// --------------------------------------------------------------------------------------- + void push_value_to_entry(vcf::Variant& gvcf_entry, size_t value, string key); void push_value_to_entry(vcf::Variant& gvcf_entry, string value, string key); void push_info_field(vcf::Variant& gvcf_entry, string value, string key); @@ -204,7 +296,7 @@ template int compare(const T1& p11, const T2& p12, const T1& p21, const T2& p22); void push_value_to_vector(vector& v, long index, const string& entry); -void extend_header(vcf::VariantCallFile &vcf); +void extend_header(vcf::VariantCallFile &vcf, bool add_subset); void parse_parameters_from_json(string filename, vcf::VariantCallFile& vcf); bool validate_filename_parameter(string filename, string param_name); diff --git a/Analysis/VariantCaller/tvcutils/validate_bed.cpp b/Analysis/VariantCaller/tvcutils/validate_bed.cpp index eb66f891..2d1d1a1c 100644 --- a/Analysis/VariantCaller/tvcutils/validate_bed.cpp +++ b/Analysis/VariantCaller/tvcutils/validate_bed.cpp @@ -48,7 +48,7 @@ void ValidateBedHelp() printf (" --validation-log FILE log file for user-readable warning/error messages [stdout]\n"); printf (" --meta-json FILE save validation and file statistics to json file [none]\n"); printf (" --unmerged-detail-bed FILE output a valid unmerged BED. To be used as input to --primer-trim-bed argument of variant_caller_pipeline.py (recommended) [none]\n"); - printf (" --unmerged-plain-bed FILE output a valid merged BED. To be used as input to --region-bed argument of variant_caller_pipeline.py (recommended) [none]\n"); + printf (" --unmerged-plain-bed FILE output a valid unmerged BED. To be used as input to --region-bed argument of variant_caller_pipeline.py (recommended) [none]\n"); printf (" --merged-detail-bed FILE output an (almost) valid bedDetail merged BED [none]\n"); printf (" --merged-plain-bed FILE output a valid plain merged BED [none]\n"); printf (" --effective-bed FILE output a valid effective BED [none]\n"); @@ -416,6 +416,10 @@ bool parse_track_line(char *track_line, int line_number, BedFile& bed) bed.log_line(kLineFixed, kUnsuppressable, line_number, 0, "track line has ionVersion higher than current ionVersion=4.0"); } + if (bed.ion_version == 0) { + bed.log_line(kLineFixed, kUnsuppressable, line_number, 0, "track line has no ionVersion number"); + } + return true; } @@ -424,23 +428,40 @@ void parse_bed_detail_targets(char *id, char *description, int line_number, int { // assert type=bedDetail + bool invalid_format = false; + string error_details; + if (bed.ion_version < 4.0) { + bed_line->gene_id = description; if (bed_line->gene_id == ".") bed_line->gene_id.clear(); + + size_t found = bed_line->gene_id.find("="); + if (found != string::npos) { + invalid_format = true; + if (not error_details.empty()) + error_details += "; "; + error_details += "For ionVersion < 4.0 GENE_ID column cannot contain an = sign"; + } + found = bed_line->gene_id.find(";"); + if (found != string::npos) { + invalid_format = true; + if (not error_details.empty()) + error_details += "; "; + error_details += "For ionVersion < 4.0 GENE_ID column cannot contain a ; character"; + } + + bed_line->submitted_region = id; if (bed_line->submitted_region == ".") bed_line->submitted_region.clear(); } else { - bool invalid_format = false; - if (description[0] == '.' and description[1] == 0) return; - string error_details; - while (*description) { if (*description == ';') { description++; @@ -451,7 +472,7 @@ void parse_bed_detail_targets(char *id, char *description, int line_number, int string key; bool invalid_char = false; for(; *description and *description != '=' and *description != ';'; ++description) { - if (isalnum(*description) or *description == '_' or *description == '.' or *description == '-') + if (isalnum(*description) or *description == '_' or *description == '.' or *description == '-' or *description == '*') key.push_back(*description); else { invalid_format = true; @@ -534,12 +555,10 @@ void parse_bed_detail_targets(char *id, char *description, int line_number, int bed_line->ion_value.push_back(value); } } - - - if (invalid_format) { - bed.log_column(kLineIgnored, kUnsuppressable, line_number, column, bed_line, "Problem parsing description column: ", error_details.c_str()); - bed_line->filtered = true; - } + } + if (invalid_format) { + bed.log_column(kLineIgnored, kUnsuppressable, line_number, column, bed_line, "Problem parsing description column: ", error_details.c_str()); + bed_line->filtered = true; } } @@ -569,7 +588,7 @@ void parse_bed_detail_hotspots(const char *id, char *description, int line_numbe // Parse names string key; for(; *id and *id != '=' and *id != ';'; ++id) { - if (isalnum(*id) or *id == '_' or *id == '.' or *id == '-') + if (isalnum(*id) or *id == '_' or *id == '.' or *id == '-' or *id == '*') key.push_back(*id); else { invalid_format = true; @@ -691,7 +710,7 @@ string validate_name(char *name, int line_number, int column, BedLine *bed_line, bool warning = false; for (; *name; ++name) { - if (isalnum(*name) or *name == '_' or *name == '.' or *name == '-' or *name == ':') + if (isalnum(*name) or *name == '_' or *name == '.' or *name == '-' or *name == ':' or *name == '*') corrected_name.push_back(*name); else warning = true; @@ -1012,8 +1031,10 @@ void merge_overlapping_regions(ReferenceReader& reference_reader, BedFile& bed) merged_line->name += A->name; merged_line->gene_id += "&"; merged_line->gene_id += A->gene_id; - merged_line->submitted_region += "&"; - merged_line->submitted_region += A->submitted_region; + if (not A->submitted_region.empty()) { + merged_line->submitted_region += "&"; + merged_line->submitted_region += A->submitted_region; + } merged_line->ref += "&"; merged_line->ref += A->ref; merged_line->obs += "&"; diff --git a/Analysis/VariantCaller/vcfcomp/vcfcomp.cpp b/Analysis/VariantCaller/vcfcomp/vcfcomp.cpp new file mode 100644 index 00000000..8c8a1da7 --- /dev/null +++ b/Analysis/VariantCaller/vcfcomp/vcfcomp.cpp @@ -0,0 +1,1638 @@ +#include +#include +#include +#include +#include +#include +#include +#include +#include +#include +#include +#include "sam.h" +//#include "kstring.h" +#include "IonVersion.h" + + +using namespace std; + +int min_mapping_qv = 0; + +map, pair > map_target_size; +map > > map_target_ranges; +void init_target_size() { + map_target_ranges.clear(); +} + +//----------------------------------------------------- +string itos(long int i) { // convert int to string + stringstream s; + s << i; + return s.str(); +} +//----------------------------------------------------- +static int depth; + +typedef struct { + int beg, end; + samfile_t *in; +} tmpstruct_t; + +// callback for bam_fetch() +static int fetch_func(const bam1_t *b, void *data) { + if (b->core.qual < min_mapping_qv) { + return 1; + } + bam_plbuf_t *buf = (bam_plbuf_t*)data; + bam_plbuf_push(b, buf); + return 0; +} +// callback for bam_plbuf_init() +static int pileup_func(uint32_t tid, uint32_t pos, int n, const bam_pileup1_t *pl, void *data) { + tmpstruct_t *tmp = (tmpstruct_t*)data; + if ((int)pos >= tmp->beg && (int)pos < tmp->end) { + //printf("%s\t%d\t%d\n", tmp->in->header->target_name[tid], pos + 1, n); + depth = n; + } + return 0; +} + +//-------------------------------------------------------- + +string vartypes[5] {"SNP","DEL","INS","MNP","OTHER"}; +inline int vartype(const string ref, const string alt) { + if(ref.length() == alt.length()) { + if(ref.length() == 1) return 0; + else return 3; + } + if(ref.length() < alt.length()) return 2; + else return 1; +} +//-------------------------------------------------------- +struct VariantFeatures { +private: + std::map * info_key_value; +public: + string vc_record; + char gt_index; + + inline int get_int(string info_key) { + if(info_key_value!=NULL) { + std::map< string , string >::iterator key_val = info_key_value->find(info_key); + if( key_val != info_key_value->end() && key_val->second.length() >0 && isdigit((key_val->second)[0])) return atoi(&((key_val->second)[0])); //dangerous but fast, potential bug + } + return -1; + } + + inline float get_float(string info_key) { + if(info_key_value!=NULL) { + std::map< string , string >::iterator key_val = info_key_value->find(info_key); + if( key_val != info_key_value->end() && key_val->second.length() >0 && (isdigit((key_val->second)[0]) || (key_val->second)[0] == '-' || (key_val->second)[0] == '+')) return atof(&((key_val->second)[0])); //dangerous but fast, potential bug + } + return -1; + } + + + VariantFeatures(string _vc_record, char _index) { + vc_record = _vc_record; + gt_index = _index; + info_key_value=NULL; + }; + ~VariantFeatures() { + if(info_key_value != NULL) delete info_key_value; + } + + void parse_info_values() { + + int col_idx = 0; + std::stringstream cols(vc_record); + string vcf_col; + while(cols.good() && getline( cols, vcf_col, '\t') && ++col_idx<6); + if(col_idx == 6) { + + if(info_key_value != NULL) delete info_key_value; + info_key_value = new std::map(); + info_key_value->insert(std::pair("QUAL", vcf_col)); + + while(cols.good() && getline( cols, vcf_col, '\t') && ++col_idx<8); + if(col_idx == 8 && !vcf_col.empty()) { + std::stringstream features(vcf_col); + string flag; + while(features.good() && getline( features, flag, ';')) { + string::size_type pos = flag.find("=",0); + if(pos!=string::npos && pos < flag.length()-1) { + int val_idx = 0; + string flag_name = flag.substr(0,pos); + std::stringstream values(flag.substr(pos+1)); + string value; + while(values.good() && getline( values, value, ',') && ++val_idx < (int)gt_index); + if(val_idx == (int)gt_index || val_idx == 1) info_key_value->insert(std::pair(flag_name, value)); // if value already exists, that means that it is repeated twice in the vcf record, we keep first copy + } + } + } + } + }; + +}; +//-------------------------------------------------------- +struct VCFinfo { +private: + vector ref; + vector alt; + vector info; + int zyg; // -1 = not available, 0 = HOM, 1 = HET/REF, 2 = HET/NON-REF + int DP; // 0 = zero or not available + long int target_end_pos; + +public: + + inline void set_dp(int dp) { + DP= dp; + } + inline void set_zyg(int _zyg) { + zyg = _zyg; + } + inline int get_zyg() { + return zyg; + } + inline void set_target_end_pos(long int pos) { + target_end_pos = pos; + } + inline long int get_target_end_pos() { + return target_end_pos; + } + + void set_dp(string _info) { + DP=0; + if(!_info.empty()) { + string::size_type pos = _info.find("DP="); + if(pos!=string::npos && _info.length()>pos+3 && isdigit(_info[pos+3])) DP = atoi(&(_info[pos+3])); // fast, but dangerous, assumes string is represented in continuous array, potential bug + } + } + + int get_var_type(int idx) { //SNP=0,DEL=1,INS=2,MNP=3,OTHER=4 + if(idx>=(int)ref.size()) { + cerr << "get_var_type(idx): requested idx is larger than allele counts" << endl; + exit(1); + } + return vartype(ref.at(idx), alt.at(idx)); + } + + float get_int_flag_value(int idx,string FLAG) { + if(idx>=(int)info.size()) { + cerr << "get_int_flag_value(idx): requested idx is larger than allele counts" << endl; + exit(1); + } + return info.at(idx).get_float(FLAG); + } + + int alt_count() { + return alt.size(); + } + const string get_alt(int idx) { + return alt.at(idx); + } + const string get_ref(int idx) { + return ref.at(idx); + } + const string get_info(int idx) { + return info.at(idx).vc_record; + } + char get_gt_index(int idx) { + return info.at(idx).gt_index; + } + + + void parse_info_values() { + for(unsigned int i=0; i0) s << alt.at(0); + for(unsigned int i=1; i0) s << ref.at(0); + for(unsigned int i=1; i added_alt; + + std::stringstream cols(line); + while(cols.good()) { + string vcf_col; + getline( cols, vcf_col, '\t'); + + switch(++col_idx) { + case 4: { + ref_al = vcf_col; + break; + } + case 5: { + std::stringstream ss(vcf_col); + while(ss.good()) { + string tmp_alt; + getline( ss, tmp_alt, ','); + alt.push_back(tmp_alt); + } + break; + } + case 8: { + //info_al = vcf_col; + break; + } + case 9: { + std::stringstream ss(vcf_col); + int gt_col = -1; + string tmp_format; + while(ss.good()) { + gt_col++; + getline( ss, tmp_format, ':'); + if(!ignore_genotype && strcmp(tmp_format.c_str(), "GT")==0) { + GT_idx = gt_col; + break; + } + } + break; + } + case 10: { + if(GT_idx==-1) break; + int tmp_idx = GT_idx; // for now GT_idx represents column index + GT_idx = -1; + std::stringstream ss(vcf_col); + int gt_col = -1; + string tmp_value; + while(ss.good()) { + gt_col++; + getline( ss, tmp_value, ':'); + if(gt_col == tmp_idx) { + if( tmp_value.empty()) break; + std::stringstream sf(tmp_value); + + char separator = '|'; + if(tmp_value.find("/",0) != string::npos) separator = '/'; + else if(tmp_value.find("|",0) == string::npos) break; + + GT_idx = 0; // now GT_idx represents allele number + + string gt_value; + vector alt_subset; + while(sf.good()) { + getline(sf, gt_value, separator); + if(gt_value.length()>0 && isdigit(gt_value[0])) { + if( gt_value[0]!='0') { + GT_idx = atoi(gt_value.c_str()); + if(GT_idx-1<(int)alt.size()) { + bool already_added = false; + for(unsigned int i=0; i 0) { + if(GT_idx == -1) { + rows_missing_genotype++; + set_zyg(-1); + } else set_zyg( (!obs_ref_allele) ? ( alleles_loaded == 1 ? 0 : 2) : 1 ); + set_dp(info_al); + + for(int i=0; i contig; + +public: + int load_file(string fasta_file) { + ifstream infile; + infile.open(fasta_file.c_str()); + string line; + + if (!infile) { + cerr << "Unable to read " << fasta_file << endl; + exit(1); + } + + int contigs_loaded = 0; + string contig_name = ""; + string sequence = ""; + while (getline(infile, line)) { + if(line[0]=='>') { + if(contig_name!="") { + int pos = contig_name.find("\t"); + if (pos != -1) {contig_name = contig_name.substr(0, pos);} + std::pair::iterator,bool> ret = contig.insert ( std::pair(contig_name, sequence)); + if (ret.second==false) { + cerr << "Contig is listed twice in fasta. Abort! " << contig_name << endl; + exit(1); + } + contigs_loaded++; + cerr << contigs_loaded << " loaded " << contig_name << " length: " << sequence.length() << endl; + } + contig_name = line.substr(1); + sequence=""; + } else if(line[0]!='#') { + sequence += line; + } + } + + if(contig_name!="") { + int pos = contig_name.find("\t"); + if (pos != -1) {contig_name = contig_name.substr(0, pos);} + std::pair::iterator,bool> ret = contig.insert ( std::pair(contig_name, sequence)); + if (ret.second==false) { + cerr << "Contig is listed twice in fasta. Abort! " << contig_name << endl; + exit(1); + } + contigs_loaded++; + cerr << contigs_loaded << " loaded " << contig_name << " length: " << sequence.length() << endl; + } + return contigs_loaded; + } + + + int size() { + return contig.size(); + } + + bool left_align_indel(string chr, long & vcf_pos, string & ref, string & alt) { + int prefsize = vcf_pos<70?vcf_pos:70; //caution: assuming that vcf_pos > 0, based on vcf-format + string prefseq = ""; + try {prefseq = contig.find(chr)->second.substr(vcf_pos - prefsize, prefsize - 1);} catch(...) {prefseq = "";} + string refseq = prefseq + ref; + string altseq = prefseq + alt; + + int reflen = refseq.length(); + int altlen = altseq.length(); + int right_match = 0; + + while( right_match < reflen && right_match < altlen && refseq[reflen - 1 - right_match]==altseq[altlen - 1 - right_match]) { + right_match++; + } + if(right_match > 0) { + long l = reflen-right_match-ref.length(); + if (l < 0) {ref = refseq.substr(0, ref.length());} + else {ref = refseq.substr(reflen-right_match-ref.length(), ref.length());} + l = altlen-right_match-alt.length(); + if (l < 0) {alt = altseq.substr(0, alt.length());} + else {alt = altseq.substr(altlen-right_match-alt.length(), alt.length());} + vcf_pos -= right_match; + return true; + } + return false; + } + +}; + +//----------------------------------------------------- +class VCFList { + std::map > vcfrec; + +public: + void remove(VCFList* p, bool zygosity_match, bool allele_match) { + if (p == NULL) {return;} + for (std::map >::iterator iter = p->vcfrec.begin(); (iter != p->vcfrec.end()); ++iter) { + for (std::map::iterator iter_pos = iter->second.begin(); (iter_pos != iter->second.end()); ++iter_pos) { + std::map >::iterator iter2 = vcfrec.find(iter->first); + if (iter2 != vcfrec.end()) { + std::map::iterator iter2_pos = iter2->second.find(iter_pos->first); + if (iter2_pos != iter2->second.end()) { + if (zygosity_match) { + if ((iter_pos->second.ref_to_str().find(iter2_pos->second.ref_to_str()) != string::npos) and (iter_pos->second.alt_to_str().find(iter2_pos->second.alt_to_str()) != string::npos)and (iter2_pos->second.get_zyg() == iter_pos->second.get_zyg())) {iter2->second.erase(iter2_pos);} + } + else if (allele_match) { + cerr << iter2_pos->second.ref_to_str() << endl; + cerr << iter_pos->second.ref_to_str() << endl; + cerr << iter2_pos->second.alt_to_str() << endl; + cerr << iter_pos->second.alt_to_str() << endl; + if ((iter_pos->second.ref_to_str().find(iter2_pos->second.ref_to_str()) != string::npos) and (iter_pos->second.alt_to_str().find(iter2_pos->second.alt_to_str()) != string::npos)) {iter2->second.erase(iter2_pos);} + } + else { + iter2->second.erase(iter2_pos); + } + } + } + } + } + } + + long calculate_target_size(int DP) { + long target_size = 0; + for (map >::iterator chrit = vcfrec.begin(); (chrit != vcfrec.end()); ++chrit) { + for (map::iterator posit = chrit->second.begin(); (posit != chrit->second.end()); ++posit) { + if (posit->second.get_dp() >= DP) { + target_size += (posit->second.get_zyg() - posit->first); + } + } + } + return target_size; + } + +private: + void add(string chr, long genpos, VCFinfo & rec) { + std::map< string , std::map >::iterator it = vcfrec.find(chr); + if(it == vcfrec.end()) { + std::map emptymap; + it = vcfrec.insert(std::pair< string , std::map > (chr, emptymap)).first; + } + + std::pair::iterator,bool> ret = (it->second).insert ( std::pair(genpos, rec)); + if (ret.second==false) ret.first->second.add(rec); + } + + bool add(const string chr, const long genpos, const string ref, const string alt, const string info, const char gt_index, const int zyg) { + std::map< string , std::map >::iterator it = vcfrec.find(chr); + if(it == vcfrec.end()) { + std::map emptymap; + it = vcfrec.insert(std::pair< string , std::map > (chr, emptymap)).first; + } + + std::map::iterator ret = (it->second).find(genpos); + if(ret == (it->second).end()) { + (it->second).insert ( std::pair(genpos, VCFinfo(ref,alt,info, gt_index, zyg))); + } else { + if(ret->second.get_zyg() > 9) { // this is used only for BED files to store second coordinate + if(ret->second.get_zyg() < zyg) ret->second.set_zyg(zyg); + } else { + for(int i=0; isecond.alt_count(); i++) { + if(strcmp(ref.c_str(),ret->second.get_ref(i).c_str())==0 && strcmp(alt.c_str(),ret->second.get_alt(i).c_str())==0) return false; + } + ret->second.add(ref, alt, info, gt_index, zyg); + } + } + return true; + } + + + void add(string vcfline, long &alleles_loaded, long &rows_missing_genotype, long &het_rows, long & hom_rows, FASTA * reference = NULL, bool split_mnp = true, bool ignore_genotype = false) { + alleles_loaded = 0; + string::size_type pos = vcfline.find("\t",0); + + if( pos != string::npos && pos > 0 && pos < vcfline.length() - 1) { + string chr = vcfline.substr(0,pos); + long genpos = atoi(vcfline.substr(pos+1).c_str()); + + // extracting genotyped/or all alleles at that position and the info + // this eliminates non-called alleles that are mixed with genotyped alleles + + VCFinfo rec(vcfline, alleles_loaded, rows_missing_genotype, ignore_genotype); + + if(rec.get_zyg()==1) het_rows++; + if(rec.get_zyg()==0) hom_rows++; + for(int i=0; i T A + while(shift < (long)ref.length()-1 && shift< (long)alt.length()-1 && ref[(long)ref.length()-1-shift]==alt[(long)alt.length()-1-shift]) shift++; + if(shift>0) { + ref = ref.substr(0, ref.length()-shift); + alt = alt.substr(0,alt.length()-shift); + } + //TA TG -> A G + shift = 0; + while(shift < (long)ref.length()-1 && shift< (long)alt.length()-1 && ref[shift]==alt[shift]) shift++; + if(shift>0) { + ref = ref.substr(shift); + alt = alt.substr(shift); + } + long adjusted_pos = genpos+shift; + + int vt = vartype(ref,alt); + + //left-align indels + if(reference!=NULL && (vt == 1 || vt == 2)) reference->left_align_indel(chr, adjusted_pos, ref, alt); + + //split-MNPs into single SNPs, this is wrong but community still does it + if(split_mnp && vt == 3) { // XXX + for(unsigned int j=0; j > * getList() { + return &vcfrec; + } +//----------------------------------------------------- + long int load_file(string filename, FASTA * reference = NULL, bool split_mnp = true, bool ignore_genotype = false) { + ifstream infile; + infile.open(filename.c_str()); + string line; + + if (!infile) { + cerr << "Unable to read " << filename << endl; + exit(1); + } + + long rows_processed = 0; + long rows_loaded = 0; + long alleles_loaded = 0, al; + long rows_missing_genotype = 0; + long het_rows = 0, hom_rows = 0; + + while (getline(infile, line)) { + if( line[0]!='#') { + add(line, al, rows_missing_genotype, het_rows, hom_rows, reference, split_mnp, ignore_genotype); + if(al>0) { + rows_loaded++; + alleles_loaded+=al; + } + rows_processed++; + } + } + + infile.close(); + + cerr << "# informative vcf records:" << rows_processed << "\n# records containing alternative allele:" << rows_loaded << "\n# loaded alternative alleles:" << alleles_loaded << endl; + cerr << "# loaded vcf records with HET genotype:" << het_rows << "\n# loaded vcf records with HOM genotype:" << hom_rows << "\n# loaded vcf records with missing genotype:" << rows_missing_genotype << endl; + return rows_loaded; + } +//----------------------------------------------------- + long int load_bed_file(string filename) { + ifstream infile; + infile.open(filename.c_str()); + string line; + + if (!infile) { + cerr << "Unable to read " << filename << endl; + exit(1); + } + + long rows_processed = 0; + long total_target_size = 0; + init_target_size(); + + while (getline(infile, line)) + if( line[0]!='#') { + string::size_type pos = line.find("\t",0); + + if( pos != string::npos && pos > 0 && pos < line.length() - 1) { + string chr = line.substr(0,pos); + long start_pos = atoi(line.substr(pos+1).c_str()); + if( (pos = line.find("\t",pos+1)) != string::npos) { + long end_pos = atoi(line.substr(pos+1).c_str()); + if(add(chr, start_pos, "", "", "", 0, end_pos)) { + total_target_size += end_pos - start_pos; + map_target_ranges[chr].push_back(make_pair(start_pos, end_pos)); + } + } + rows_processed++; + } + } + + infile.close(); + + cerr << "# informative bed records:" << rows_processed << "\n# total target size:" << total_target_size << endl; + return rows_processed; + } + +// Supports overlapping target segments (on fly merging) +//----------------------------------------------------- + long get_target_vcf(VCFList * vcf_in, VCFList * vcf_out) { + + long records_on_target = 0; + for (std::map< string , std::map >::iterator bed_chrit = vcfrec.begin(); bed_chrit != vcfrec.end(); ++bed_chrit) { + + std::map< string , std::map >::iterator vcf_chrit = vcf_in->getList()->find(bed_chrit->first); + if(vcf_chrit == vcf_in->getList()->end()) continue; + + std::map::iterator vcf_posit = vcf_chrit->second.begin(); + + std::map::iterator bed_posit = bed_chrit->second.begin(); + + if(bed_posit == bed_chrit->second.end()) continue; + + long int reg_start = bed_posit->first; + long int reg_end = bed_posit->second.get_zyg(); + + bed_posit++; + + while (bed_posit != bed_chrit->second.end()) { + if( vcf_posit == vcf_chrit->second.end()) break; + + if(bed_posit->first <= reg_end) { + if(bed_posit->second.get_zyg() > reg_end ) reg_end = bed_posit->second.get_zyg(); + bed_posit++; + continue; + } + + if( reg_end < vcf_posit->first) { + reg_start = bed_posit->first; + reg_end = bed_posit->second.get_zyg(); + bed_posit++; + continue; + } + + while( vcf_posit != vcf_chrit->second.end() && reg_start >= vcf_posit->first) vcf_posit++; + while( vcf_posit != vcf_chrit->second.end() && reg_end >= vcf_posit->first) { + if((vcf_posit->first + (long)vcf_posit->second.get_ref(0).length()-1) <= reg_end) { + vcf_posit->second.set_target_end_pos(reg_end - vcf_posit->first); + vcf_out->add(vcf_chrit->first, vcf_posit->first, vcf_posit->second); + records_on_target++; + } + vcf_posit++; + } + } + + while( vcf_posit != vcf_chrit->second.end() && reg_start >= vcf_posit->first) vcf_posit++; + while( vcf_posit != vcf_chrit->second.end() && reg_end >= vcf_posit->first) { + if(vcf_posit->first + (long)vcf_posit->second.get_ref(0).length()-1 <= reg_end) { + vcf_posit->second.set_target_end_pos(reg_end - vcf_posit->first); + vcf_out->add(vcf_chrit->first, vcf_posit->first, vcf_posit->second); + records_on_target++; + } + vcf_posit++; + } + } + return records_on_target; + } +//----------------------------------------------------- + long merge_overlapping_segments(VCFList * merged_bed) { + long total_target_size = 0; + init_target_size(); + for (std::map< string , std::map >::iterator bed_chrit = vcfrec.begin(); bed_chrit != vcfrec.end(); ++bed_chrit) { + std::map::iterator bed_posit = bed_chrit->second.begin(); + + if(bed_posit == bed_chrit->second.end()) continue; + + long int reg_start = bed_posit->first; + long int reg_end = bed_posit->second.get_zyg(); + + bed_posit++; + + while (bed_posit != bed_chrit->second.end()) { + + if(bed_posit->first <= reg_end) { + if(bed_posit->second.get_zyg() > reg_end ) reg_end = bed_posit->second.get_zyg(); + bed_posit++; + continue; + } + + merged_bed->add(bed_chrit->first,reg_start, "", "", "", 0, reg_end); + total_target_size+= reg_end - reg_start; + map_target_ranges[bed_chrit->first].push_back(make_pair(reg_start, reg_end)); + + reg_start = bed_posit->first; + reg_end = bed_posit->second.get_zyg(); + bed_posit++; + + } + + merged_bed->add(bed_chrit->first,reg_start, "", "", "", 0, reg_end); + total_target_size+= reg_end - reg_start; + map_target_ranges[bed_chrit->first].push_back(make_pair(reg_start, reg_end)); + } + + return total_target_size; + } +//----------------------------------------------------- + long intersect(VCFList * in, VCFList * bed_out) { + long total_target_size = 0; + init_target_size(); + for (std::map< string , std::map >::iterator chrit = vcfrec.begin(); chrit != vcfrec.end(); ++chrit) { + std::map< string , std::map >::iterator in_chrit = in->getList()->find(chrit->first); + if(in_chrit != in->getList()->end()) + for (std::map::iterator posit = chrit->second.begin(), in_posit = in_chrit->second.begin(); posit != chrit->second.end() && in_posit != in_chrit->second.end(); ) { + if(posit->first > in_posit->second.get_zyg()) { + in_posit++; + continue; + } + if(in_posit->first > posit->second.get_zyg()) { + posit++; + continue; + } + long reg_start = in_posit->first < posit->first ? posit->first : in_posit->first; + long reg_end = posit->second.get_zyg() < in_posit->second.get_zyg() ? posit->second.get_zyg() : in_posit->second.get_zyg(); + total_target_size += reg_end - reg_start; + map_target_ranges[in_chrit->first].push_back(make_pair(reg_start, reg_end)); + bed_out->add(chrit->first, reg_start, "", "", "", 0, reg_end); + if(posit->second.get_zyg() == reg_end) posit++; + else in_posit++; + } + } + return total_target_size; + } +//----------------------------------------------------- + void parse_info_values() { + for(std::map< string , std::map >::iterator chrit = vcfrec.begin(); chrit != vcfrec.end(); ++chrit) + for(std::map::iterator posit = chrit->second.begin(); posit != chrit->second.end(); posit++) posit->second.parse_info_values(); + } + +//----------------------------------------------------- + int * get_var_counts(string basename = "", int DP = -1) { + ofstream output; + bool outfile = (basename!=""); + if(outfile)output.open(basename.c_str()); + if(!output) { + cerr << "Error writing output file: " << basename << endl; + } + + int * counts = new int[5] {0,0,0,0,0}; + for(std::map< string , std::map >::iterator chrit = vcfrec.begin(); chrit != vcfrec.end(); ++chrit) + for(std::map::iterator posit = chrit->second.begin(); posit != chrit->second.end(); posit++) + for(int i=0; i< posit->second.alt_count(); i++) { + int vartype = posit->second.get_var_type(i); + if(DP==-1 || posit->second.get_dp()>=DP) counts[vartype]++; + if(output) output << chrit->first << "\t" << posit->first << "\t*"<< vartypes[vartype] <<"*\t" << posit->second.get_ref(i) << "\t" << posit->second.get_alt(i)<< "\t"<< posit->second.get_dp() << "\t.\t" << posit->second.get_info(i) << endl; + } + + if(outfile) output.close(); + + return counts; + } +//----------------------------------------------------- + int ** get_var_counts(string FLAG, float _min, float _step, int nbins, string FLAG2="", char cmp='>', float _val=0) { + float _max = _min + nbins*_step; + + int ** counts = new int*[2]; + counts[0] = new int[nbins+3]; + counts[1] = new int[nbins+3]; + + for(int i=0; i >::iterator chrit = vcfrec.begin(); chrit != vcfrec.end(); ++chrit) + for(std::map::iterator posit = chrit->second.begin(); posit != chrit->second.end(); posit++) + for(int i=0; i < posit->second.alt_count(); i++) { + float flag_value = posit->second.get_int_flag_value(i,FLAG); + int vartype = posit->second.get_var_type(i) > 0; + + if(flag_value == -1 || (!FLAG2.empty() && ((cmp=='<' && posit->second.get_int_flag_value(i,FLAG2)<_val) || (cmp=='>' && posit->second.get_int_flag_value(i,FLAG2)>_val)))) counts[vartype][nbins+2]++; + else if(flag_value<_min) counts[vartype][0]++; + else if(flag_value >= _max) counts[vartype][nbins+1]++; + else + counts[vartype][1+(int)((flag_value-_min)/_step)]++; + } + + return counts; + } +//----------------------------------------------------- + + void positional_allele_match(VCFList * a, VCFList * match, VCFList * unmatch1, VCFList * unmatch2, bool require_allele_match, bool require_zygosity_match) { + for (std::map< string , std::map >::iterator chrit = vcfrec.begin(); chrit != vcfrec.end(); ++chrit) { + std::map< string , std::map >::iterator achrit = a->getList()->find(chrit->first); + if(achrit != a->getList()->end()) { + std::map::iterator posit = chrit->second.begin(), aposit = achrit->second.begin(); + while(posit != chrit->second.end() && aposit != achrit->second.end()) { + if(posit->first < aposit->first) { + unmatch1->add(chrit->first,posit->first, posit->second); + posit++; + continue; + } else if(posit->first > aposit->first) { + unmatch2->add(achrit->first,aposit->first, aposit->second); + aposit++; + continue; + } else { + + if(!require_allele_match) { + if(!require_zygosity_match || (aposit->second.get_zyg() != -1 && posit->second.get_zyg() != -1 && aposit->second.get_zyg() == posit->second.get_zyg() )) + match->add(achrit->first,aposit->first, aposit->second); + else { + unmatch1->add(chrit->first,posit->first, posit->second); + unmatch2->add(achrit->first,aposit->first, aposit->second); + } + } else { + // allele matching + int n_match_alleles = 0; + for(int i=0; i < posit->second.alt_count(); i++) { + bool has_match = false; + for(int ai=0; ai < aposit->second.alt_count(); ai++) + if(strcmp(aposit->second.get_alt(ai).c_str(),posit->second.get_alt(i).c_str()) == 0) { + if(!require_zygosity_match || (aposit->second.get_zyg() != -1 && posit->second.get_zyg() != -1 && aposit->second.get_zyg() == posit->second.get_zyg() )) + match->add(achrit->first,aposit->first, aposit->second.get_ref(ai), aposit->second.get_alt(ai), aposit->second.get_info(ai), aposit->second.get_gt_index(ai), aposit->second.get_zyg()); + else { + unmatch1->add(chrit->first,posit->first, posit->second.get_ref(i), posit->second.get_alt(i), posit->second.get_info(i), posit->second.get_gt_index(i), posit->second.get_zyg()); + unmatch2->add(achrit->first,aposit->first, aposit->second.get_ref(ai), aposit->second.get_alt(ai), aposit->second.get_info(ai), aposit->second.get_gt_index(ai), aposit->second.get_zyg()); + } + has_match = true; + n_match_alleles++; + // break; + } + if(!has_match) unmatch1->add(chrit->first,posit->first, posit->second.get_ref(i), posit->second.get_alt(i), posit->second.get_info(i), posit->second.get_gt_index(i), posit->second.get_zyg()); + } + + if(n_match_allelessecond.alt_count()) + for(int ai=0; ai < aposit->second.alt_count(); ai++) { + bool has_match = false; + for(int i=0; i < posit->second.alt_count(); i++) + if(strcmp(aposit->second.get_alt(ai).c_str(),posit->second.get_alt(i).c_str()) == 0) { + has_match = true; + break; + } + if(!has_match) unmatch2->add(achrit->first,aposit->first, aposit->second.get_ref(ai), aposit->second.get_alt(ai), aposit->second.get_info(ai), aposit->second.get_gt_index(ai), aposit->second.get_zyg()); + } + + } + + ++posit; + ++aposit; + } + } + while(posit != chrit->second.end()) { + unmatch1->add(chrit->first,posit->first, posit->second); + posit++; + } + while(aposit != achrit->second.end()) { + unmatch2->add(achrit->first,aposit->first, aposit->second); + aposit++; + } + } else { + for(std::map::iterator posit = chrit->second.begin(); posit != chrit->second.end(); posit++) { + unmatch1->add(chrit->first,posit->first, posit->second); + } + } + } + + for (std::map< string , std::map >::iterator achrit = a->getList()->begin(); achrit != a->getList()->end(); ++achrit) { + std::map< string , std::map >::iterator chrit = vcfrec.find(achrit->first); + if(chrit == vcfrec.end()) { + for(std::map::iterator aposit = achrit->second.begin(); aposit != achrit->second.end(); aposit++) + unmatch2->add(achrit->first,aposit->first, aposit->second); + } + } + } +//----------------------------------------------------- + + void match(VCFList * a, VCFList * tp, VCFList * fn, VCFList * fp, FASTA * reference, bool require_allele_match, bool require_zygosity_match) { + positional_allele_match( a, tp, fn, fp, require_allele_match, require_zygosity_match); + // add haplotype, block, hp-error matchings + } + +//----------------------------------------------------- + + void set_positional_info_from_bam(const char * fname) { + if (strlen(fname) == 0) { + return; + } + tmpstruct_t tmp; + + tmp.beg = 0; + tmp.end = 0x7fffffff; + tmp.in = samopen(fname, "rb", 0); + if (tmp.in == 0) { + fprintf(stderr, "Fail to open BAM file %s\n", fname); + return; + } + + int ref; + bam_index_t *idx=NULL; + bam_plbuf_t *buf=NULL; + idx = bam_index_load(fname); // load BAM index + if (idx == 0) { + fprintf(stderr, "BAM indexing file is not available.\n"); + return; + } + + + for(std::map< string , std::map >::iterator chrit = vcfrec.begin(); chrit != vcfrec.end(); ++chrit) + for(std::map::iterator posit = chrit->second.begin(); posit != chrit->second.end(); posit++) + for(int i=0; i< posit->second.alt_count(); i++) { + depth = -1; + string pos_id = chrit->first + ":" + itos(posit->first)+ "-" + itos(posit->first); + bam_parse_region(tmp.in->header, pos_id.c_str(), &ref, + &tmp.beg, &tmp.end); // parse the region + if (ref < 0) { + cerr <<"Invalid region " << pos_id << endl; + return; + } + buf = bam_plbuf_init(pileup_func, &tmp); // initialize pileup + bam_fetch(tmp.in->x.bam, idx, ref, tmp.beg, tmp.end, buf, fetch_func); + bam_plbuf_push(0, buf); // finalize pileup + posit->second.set_dp(depth); + } + bam_index_destroy(idx); + bam_plbuf_destroy(buf); + + samclose(tmp.in); + } + +//----------------------------------------------------- + + void set_dp_to_zero() { + + for(std::map< string , std::map >::iterator chrit = vcfrec.begin(); chrit != vcfrec.end(); ++chrit) + for(std::map::iterator posit = chrit->second.begin(); posit != chrit->second.end(); posit++) + posit->second.set_dp(0); + } + +}; + +//----------------------------------------------------------- +void print_stats(VCFList * target, VCFList * tp, VCFList * fp, VCFList * fn, string out_dir, + string out_pref, string out_format, string json_file = "", string DP = "", + long TARGET_SIZE = -1, string full_version_string="0.0") +{ + if(tp==NULL) return; + + std::map map_fp_counts; + for (int index = 0; (index < 100); ++index) {map_fp_counts[index] = 0;} + if (fp != NULL) { + for (std::map >::iterator iter1 = fp->getList()->begin(); (iter1 != fp->getList()->end()); ++iter1) { + for (std::map::iterator iter2 = iter1->second.begin(); (iter2 != iter1->second.end()); ++iter2) { + if (iter2->second.get_target_end_pos() < 100) { + map_fp_counts[iter2->second.get_target_end_pos()]++; + } + } + } + } + std::map map_fn_counts; + for (int index = 0; (index < 100); ++index) {map_fn_counts[index] = 0;} + if (fn != NULL) { + for (std::map >::iterator iter1 = fn->getList()->begin(); (iter1 != fn->getList()->end()); ++iter1) { + for (std::map::iterator iter2 = iter1->second.begin(); (iter2 != iter1->second.end()); ++iter2) { + if (iter2->second.get_target_end_pos() < 100) { + map_fn_counts[iter2->second.get_target_end_pos()]++; + } + } + } + } + ofstream fout; + fout.open((out_dir + "/distance_from_end.txt").c_str()); + fout << "#distance_from_target_end\tfp\tfn" << endl; + for (int index = 0; (index < 100); ++index) {fout << (index * -1) << "\t" << map_fp_counts[index] << "\t" << map_fn_counts[index] << endl;} + fout.close(); + + bool text = (strcmp(out_format.c_str(),"text") == 0); + bool html = (strcmp(out_format.c_str(),"html") == 0); + + vector cov; + cov.push_back(0); + + if(!DP.empty()) { + + std::stringstream ss(DP); + string tmp_value; + while(ss.good()) { + getline( ss, tmp_value, ','); + try { + int c = atoi(tmp_value.c_str()); + if(c>0) cov.push_back(c); + } catch(...) {} + } + } + + ofstream output; + bool outfile = (json_file!=""); + if(outfile) { + output.open(json_file.c_str()); + output << "{" << endl; + } + + for(unsigned int j=0; j=" << cov[j] <<"x:SNP,DEL,INS,MNP,SNP+MNP,INDEL,ALL" << (html?"
\n":"\n"); + + int * counts_tp = (j==0) ? tp->get_var_counts(out_pref+"_tp.vcf") : tp->get_var_counts("",cov[j]); + if(j==0) { + if(html) cout << "TP:"; + else cout << "("<< out_pref << "_tp.vcf" << ")TP:"; + } else cout << "TP:"; + + for(int i=0; i<4; i++) cout << counts_tp[i] << ","; + cout << (counts_tp[0] + counts_tp[3]) << ","; + cout << (counts_tp[1] + counts_tp[2]) << ","; + cout << (counts_tp[1] + counts_tp[0] + counts_tp[2] + counts_tp[3] ) << (html?"
\n":"\n"); + if(outfile) { + output << "\"TP-SNP-" << cov[j] << "\":"<< counts_tp[0] << "," << endl; + output << "\"TP-DEL-" << cov[j] << "\":"<< counts_tp[1] << "," << endl; + output << "\"TP-INS-" << cov[j] << "\":"<< counts_tp[2] << "," << endl; + output << "\"TP-MNP-" << cov[j] << "\":"<< counts_tp[3] << "," << endl; + output << "\"TP-SNPMNP-" << cov[j] << "\":"<< (counts_tp[0] + counts_tp[3]) << "," << endl; + output << "\"TP-INDEL-" << cov[j] << "\":"<< (counts_tp[1] + counts_tp[2]) << "," << endl; + output << "\"TP-ALL-" << cov[j] << "\":"<< (counts_tp[1] + counts_tp[0] + counts_tp[2] + counts_tp[3]) << "," << endl; + } + + if( fn != NULL && fp != NULL) { + + int * counts_fn = (j==0) ? fn->get_var_counts(out_pref+"_fn.vcf") : fn->get_var_counts("",cov[j]); + + if(j==0) { + if(html) cout << "FN:"; + else cout << "("<< out_pref << "_fn.vcf" << ")FN:"; + } else cout << "FN:"; + + for(int i=0; i<4; i++) cout << counts_fn[i] << ","; + cout << (counts_fn[0] + counts_fn[3]) << ","; + cout << (counts_fn[1] + counts_fn[2]) << ","; + cout << (counts_fn[1] + counts_fn[0] + counts_fn[2] + counts_fn[3] ) << (html?"
\n":"\n"); + + if(outfile) { + output << "\"FN-SNP-" << cov[j] << "\":"<< counts_fn[0] << "," << endl; + output << "\"FN-DEL-" << cov[j] << "\":"<< counts_fn[1] << "," << endl; + output << "\"FN-INS-" << cov[j] << "\":"<< counts_fn[2] << "," << endl; + output << "\"FN-MNP-" << cov[j] << "\":"<< counts_fn[3] << "," << endl; + output << "\"FN-SNPMNP-" << cov[j] << "\":"<< (counts_fn[0] + counts_fn[3]) << "," << endl; + output << "\"FN-INDEL-" << cov[j] << "\":"<< (counts_fn[1] + counts_fn[2]) << "," << endl; + output << "\"FN-ALL-" << cov[j] << "\":"<< (counts_fn[1] + counts_fn[0] + counts_fn[2] + counts_fn[3]) << "," << endl; + } + + + int * counts_fp = (j==0) ? fp->get_var_counts(out_pref+"_fp.vcf") : fp->get_var_counts("",cov[j]); + if(j==0) { + if(html) cout << "FP:"; + else cout << "("<< out_pref << "_fp.vcf" << ")FP:"; + } else cout << "FP:"; + + for(int i=0; i<4; i++) cout << counts_fp[i] << ","; + cout << (counts_fp[0] + counts_fp[3]) << ","; + cout << (counts_fp[1] + counts_fp[2]) << ","; + cout << (counts_fp[1] + counts_fp[0] + counts_fp[2] + counts_fp[3] ) << (html?"
\n":"\n"); + + if(outfile) { + output << "\"FP-SNP-" << cov[j] << "\":"<< counts_fp[0] << "," << endl; + output << "\"FP-DEL-" << cov[j] << "\":"<< counts_fp[1] << "," << endl; + output << "\"FP-INS-" << cov[j] << "\":"<< counts_fp[2] << "," << endl; + output << "\"FP-MNP-" << cov[j] << "\":"<< counts_fp[3] << "," << endl; + output << "\"FP-SNPMNP-" << cov[j] << "\":"<< (counts_fp[0] + counts_fp[3]) << "," << endl; + output << "\"FP-INDEL-" << cov[j] << "\":"<< (counts_fp[1] + counts_fp[2]) << "," << endl; + output << "\"FP-ALL-" << cov[j] << "\":"<< (counts_fp[1] + counts_fp[0] + counts_fp[2] + counts_fp[3]) << "," << endl; + } + int target_size = 0; + if (target != NULL) { + target_size = ((j==0) ? target->calculate_target_size(0) : target->calculate_target_size(cov[j])); + } + float snp_sens = (10000*(counts_tp[0] + counts_tp[3])/(counts_tp[0] + counts_tp[3] + counts_fn[0] + counts_fn[3]+0.0001))/100.00; + float indel_sens = (10000*(counts_tp[1] + counts_tp[2])/(counts_tp[1] + counts_tp[2] + counts_fn[1] + counts_fn[2]+0.0001))/100.00; + float total_sens = (10000*(counts_tp[0] + counts_tp[3]+ counts_tp[1] + counts_tp[2])/(counts_tp[0] + counts_tp[3] + counts_tp[1] + counts_tp[2] + counts_fn[0] + counts_fn[3] + counts_fn[1] + counts_fn[2]+0.0001))/100.00; + float snp_ppv = (10000*(counts_tp[0] + counts_tp[3])/(counts_tp[0] + counts_tp[3] + counts_fp[0] + counts_fp[3]+0.0001))/100.00; + float indel_ppv = (10000*(counts_tp[1] + counts_tp[2])/(counts_tp[1] + counts_tp[2] + counts_fp[1] + counts_fp[2]+0.0001))/100.00; + float total_ppv = (10000*(counts_tp[0] + counts_tp[3]+ counts_tp[1] + counts_tp[2])/(counts_tp[0] + counts_tp[3] + counts_tp[1] + counts_tp[2] + counts_fp[0] + counts_fp[3] + counts_fp[1] + counts_fp[2]+0.0001))/100.00; + float snp_fpr = ((1.0e+8)*(counts_fp[0] + counts_fp[3])/(target_size+0.0001))/100.00; + float indel_fpr = ((1.0e+8)*(counts_fp[1] + counts_fp[2])/(target_size+0.0001))/100.00; + float total_fpr = ((1.0e+8)*(counts_fp[0] + counts_fp[3]+counts_fp[1] + counts_fp[2])/(target_size+0.0001))/100.00; + + + cout << "SENS%:"<<"-,-,-,-,-," << snp_sens << "," << indel_sens << "," << total_sens << (html?"
\n":"\n"); + cout << "PPV%:"<<"-,-,-,-,-," << snp_ppv << "," << indel_ppv << "," << total_ppv << (html?"
\n":"\n"); + if (target_size>0) cout << "FP/Mb:"<<"-,-,-,-,-," << snp_fpr << "," << indel_fpr << "," << total_fpr << (html?"
\n":"\n"); + if ((target != NULL) and (cov.size() > 1)) { + cout << "COVERAGE:" << ((j==0) ? target->calculate_target_size(0) : target->calculate_target_size(cov[j])) << (html?"
\n":"\n"); + } + + if(outfile) { + output << "\"SENS-SNPMNP" << cov[j] << "\":"<< snp_sens << "," << endl; + output << "\"SENS-INDEL" << cov[j] << "\":"<< indel_sens << "," << endl; + output << "\"SENS-ALL" << cov[j] << "\":"<< total_sens << "," << endl; + output << "\"PPV-SNPMNP" << cov[j] << "\":"<< snp_ppv << "," << endl; + output << "\"PPV-INDEL" << cov[j] << "\":"<< indel_ppv << "," << endl; + output << "\"PPV-ALL" << cov[j] << "\":"<< total_ppv << "," << endl; + if(TARGET_SIZE>0) { + output << "\"FPR-SNPMNP" << cov[j] << "\":"<< snp_fpr << "," << endl; + output << "\"FPR-INDEL" << cov[j] << "\":"<< indel_fpr << "," << endl; + output << "\"FPR-ALL" << cov[j] << "\":"<< total_fpr << "," << endl; + } + } + + delete counts_fp; + delete counts_fn; + } + + delete counts_tp; + } + + if(outfile) { + output << "\"vcfcomp-version\":"<< "\"" << full_version_string << "\""<< "\n}" << endl; + output.close(); + } + +} + +void optimizer(VCFList * _tp,VCFList * _tp_filter,VCFList * _fp, VCFList * _fp_filter, string FLAG="QUAL", float _min = 15, float _step = 5, int nbins = 10, string FLAG2="", char cmp_operator='<', float _val = 0) { + +// RBI - filter_unusual_predictions +// MLLD - data_qual_string +// STB - xx_strand_bias +// STBP - xx_strand_bias_pval + + /* + ofstream output; + bool outfile = (basename!=""); + if(outfile)output.open(basename.c_str()); + if(!output) { cerr << "Error writing output file: " << basename << endl; } + */ + + int ** tp = FLAG2.empty() ? _tp->get_var_counts(FLAG, _min, _step, nbins): _tp->get_var_counts(FLAG, _min, _step, nbins, FLAG2, cmp_operator, _val); + int ** fn = FLAG2.empty() ? _tp_filter->get_var_counts(FLAG, _min, _step, nbins) : _tp_filter->get_var_counts(FLAG, _min, _step, nbins, FLAG2, cmp_operator, _val); + int ** fp = FLAG2.empty() ? _fp->get_var_counts(FLAG, _min, _step, nbins) : _fp->get_var_counts(FLAG, _min, _step, nbins, FLAG2, cmp_operator, _val); + int ** tn = FLAG2.empty() ? _fp_filter->get_var_counts(FLAG, _min, _step, nbins) : _fp_filter->get_var_counts(FLAG, _min, _step, nbins, FLAG2, cmp_operator, _val); + + //if(outfile) { + if(!FLAG2.empty()) cout << "fixing " << FLAG2 << (char)cmp_operator << _val << endl; + cout << "thresholding " << FLAG << " min="<< _min << " step=" << _step << " nbins=" << nbins << endl; + + cout << "FLAG" << "\t" << FLAG << "\t" << "true-snp" << "\t" << "true-indel" << "\t" << "fp-snp" << "\t" << "fp-indel" << "\t" << "tn-snp" << "\t" << "tn-indel" << endl; + int i=0; + cout << FLAG << "<<\t" << _min << "\t" << (tp[0][i] + fn[0][i]) << "\t" << (tp[1][i] + fn[1][i]) << "\t" << fp[0][i] << "\t" << fp[1][i] << "\t" << tn[0][i] << "\t" << tn[1][i] << endl; + for(i=1; i<=nbins+1; i++) cout << FLAG << ">=\t" << (((float)(i-1)*_step + _min)) << "\t" << (tp[0][i] + fn[0][i]) << "\t" << (tp[1][i] + fn[1][i]) << "\t" << fp[0][i] << "\t" << fp[1][i] << "\t" << tn[0][i] << "\t" << tn[1][i] << endl; + cout << FLAG << "\t" << "N/A\t" << (tp[0][i] + fn[0][i]) << "\t" << (tp[1][i] + fn[1][i]) << "\t" << fp[0][i] << "\t" << fp[1][i] << "\t" << tn[0][i] << "\t" << tn[1][i] << endl; + + //output.close(); + //} +} + +//-------------------------------------------------- +void print_usage(int ext_code, string full_version_string) { + cerr << "*****************************************************************" << endl; + cerr << "VCF comparison tool v." << full_version_string << endl; + cerr << "Copyright (C) 2015 Ion Torrent Systems, Inc. All Rights Reserved" << endl; + cerr << "The tool generates variant matching report in form of TP,FP,FN " << endl; + cerr << "printed to stdout, and creates corresponding files, e.g., _tp.vcf" << endl; + cerr << "column 3 and 6 in the output files are for var type and coverage." << endl; + cerr <<"******************************************************************" << endl; + cerr << "Usage: vcfcomp --main-vcf filename [--option value]" << endl << endl; + cerr << "[Options]:" << endl; + cerr << "--main-vcf filename this option is required. filename points to a file with variants in VCF format (requires at minimum first 5 VCF columns," << endl; + cerr << " # columns definitions are not required). if record is missing GT value, then all alternative alleles at that position are" << endl; + cerr << " evaluated disregarding zygosity. If record contains INFO field then key=value pairs are extracted for custom reports. If"<< endl; + cerr << " other optional vcf files are not provided, then metrics related to this file only, as well intersection with target(s) are" << endl; + cerr << " are reported in TP category in (_tp.vcf)." << endl; + cerr << "--truth-vcf filename filename with variants in VCF format (requires at minimum first 5 VCF columns). if genotype GT values are missing, then" << endl; + cerr << " all alternative alleles at the same position are evaluated disregarding zygosity. variants from main-vcf are compared to" << endl; + cerr << " variants in truth-vcf. matching variants are reported as TP in (_tp.vcf). variants in main-vcf, but not in truth-vcf are" << endl; + cerr << " reported as FP in (_fp.vcf). variants in truth-vcf, but not in main-vcf are reported as FN in (_fn.vcf)." << endl; + cerr << "--bg-vcf filename filename with variants in VCF format (requires at minimum first 5 VCF columns). if genotype GT values are missing, then" << endl; + cerr << " all alternative alleles at the same position are evaluated. this file is intended for exclusion of known back-ground variants"<< endl; + cerr << " or systematic errors from variant matching step. If trut-vcf is not provided then bg-vcf is used instead." << endl; + cerr << "--filter-vcf filename filename with variants in VCF format (requires at minimum first 5 VCF columns). if genotype GT values are missing, then" << endl; + cerr << " all alternative alleles at the same position are evaluated. it should include candidate variants filtered by variant caller."<< endl; + cerr << " it is in parameter optimization report." << endl; + cerr << "--target bedfile bedfile is a file with a set of target regions in BED format. limit analysis to variants with anchor base in the target region." << endl; + cerr << " In case of DEL or block-substitution entier block should be inside the target. this option can be used multiple times. vcf files" << endl; + cerr << " are normalized then intersected with all provided target files." << endl; + cerr << "--mt,--mb [a,z,p] variant matching criteria in addition to genomic position when comparing main-vcf with truth-vcf (--mt), and bg-vcf (--mb)." << endl; + cerr << " value (a) requires alternative allele match, (z) requires allele + zygosity match, (p) position only. Default '--mt a', and '--mb p'." << endl; + cerr << "--opref prefix preffix is appended to each output file, i.e., prefix_tp.vcf, prefix_fp.vcf, prefix_fn.vcf." << endl; + cerr << "--odir path output directory. default value '.'" << endl; + cerr << "--oformat format format can be text or html. analysis numbers are printed to standard output in convenient representation form." << endl; + cerr << "--ojson filename outputs stats into the filename in a json KEY:VALUE format"<< endl; + cerr << "--main-bam filename filename of the indexed BAM file. using this option may significantly impact the runtime. this file is used to recalculate" << endl; + cerr << " certain metrics for variant positions in case they are requested by analysis, but not provided in INFO field of main-vcf," << endl; + cerr << " or values from INFO field require to be recalculated (e.g., depth of coverage metric 'DP')." << endl; + cerr << "--min_mapping_qv The minimum mapping quality to use in calculating coverage." << endl; + cerr << "--depth cov_list additional coverage depth thresholds used in the report. multiple thresholds should be comma separated. for TP,FP reports" << endl; + cerr << " DP is extracted from main-vcf INFO field if available. for FN reports DP is recalulated from the main-bam file if provided." << endl; + cerr << " missing DP equals to DP=0. example: '-dp 20,30,50'. analysis for coverage >=0 is always reported and stored in the output files." << endl; + cerr << "--fasta reference filename with the reference sequence in FASTA format. If provided, then variant maching is done by normalizing variants to" << endl; + cerr << " the reference. that enables matching of differently represented equivalent variants. usually variants representation is already" << endl; + cerr << " reference normalized, thus this option may not affect the results, however if used it will significantly increase the runtime." << endl; + cerr << "--ignore-gt [m,t,b,f] ignore GT value when it is specified in (m)ain-vcf, (t)ruth-vcf, (b)g-vcf, (f)ilter-vcf. all listed alleles are loaded, zygosity" << endl; + cerr << " is ignored." << endl; + cerr << " intended to include into comparison alleles with empty ./. or reference 0/0 GT values. example: --ignore-gt mt." << endl; + cerr << "--mnp [split,keep] splits MNPs into single SNPs or keeps them unsplit. default '--mnp split'." << endl; + cerr << "--optimze expression use only with --filter-vcf. groups variants into bins defined by expression \"FLAG1,min,step,nbins:FLAG2,<|>,value\". FLAG2 is optional." << endl; + cerr << " variants are grouped into nbins+2 where first and last bins are for values with FLAG =(min+step*nbins). FLAG value is extrated" << endl; + cerr << " from vcf records, and it goes into N/A bin if missing. Most common optimization FLAGS are QUAL, MLLD, RBI, VARB, STB, STBP, FSAF, FSAR." << endl; + cerr << " FLAG2 is used to provide a single value. Example: \"MLLD,5,1,10:QUAL,>,15\" or \"VARB,0.1,0.01,20\" . Outputs table to std output. Multiple" << endl; + cerr << " expressions can be provided by separating them with ';', e.g. \"MLLD,5,1,10:QUAL,>,15;VARB,0.1,0.01,20\" ." << endl; + cerr << "in progress: combining vars, haplotype sequence match, approx var match up to hp-error, handling block-substitutions" << endl; + cerr << endl; + + exit(ext_code); +} +//-------------------------------------------------- +int main(int argc, char *argv[]) { + + string full_version_string = IonVersion::GetVersion() + "." +IonVersion::GetRelease() + + " (" + IonVersion::GetGitHash() + ") (" + IonVersion::GetBuildNum() + ")"; + +// Set default values + string bg_vcf_file = ""; + string truth_vcf_file = ""; + string main_vcf_file = ""; + string filter_vcf_file = ""; + string ref_file = ""; + string out_pref = ""; + string out_dir = "."; + string out_format = "text"; // {text,html,json,cvs} + string DP = ""; + string main_bam_file = ""; + string ignore_genotype = "f"; + string bg_match_criteria = ""; + string truth_match_criteria = "a"; + string optimize = ""; + string map_quality = "4"; + + vector targets; + bool split_mnp = true; + string json_file =""; + +// Read input options + int acnt = 1; + while(acntload_file(ref_file); + cerr << "loaded " << ncnt << " reference contigs." << endl; + } + + if(reference==NULL || reference->size()==0) { + cerr << "\nMissing reference data." << endl; + cerr << "Reference normalization is not performed." <load_file(main_vcf_file, reference, split_mnp, !ignore_genotype.empty() && ignore_genotype.find("m")!=string::npos); + } + + if(!truth_vcf_file.empty()) { + cerr << "\nLoading truth variants: " << truth_vcf_file << endl; + varTruth = new VCFList(); + varTruth->load_file(truth_vcf_file, reference, split_mnp, !ignore_genotype.empty() && ignore_genotype.find("t")!=string::npos); + } + + + if(!bg_vcf_file.empty()) { + cerr << "\nLoading background variants: " << bg_vcf_file << endl; + varBg = new VCFList(); + varBg->load_file(bg_vcf_file, reference, split_mnp, !ignore_genotype.empty() && ignore_genotype.find("b")!=string::npos); + } + + if(!filter_vcf_file.empty()) { + cerr << "\nLoading filtered-candidate variants: " << filter_vcf_file << endl; + varFilter = new VCFList(); + varFilter->load_file(filter_vcf_file, reference, split_mnp, !ignore_genotype.empty() && ignore_genotype.find("f")!=string::npos); + } + + long TARGET_SIZE = -1; + + VCFList * target = NULL; + if(targets.size()>0) { // intersect variant list with target regions + + for(unsigned int i=0; iload_bed_file(targets.at(i)); + + //merging target is required for correct target size calculation and intersection of multiple BEDs + //get_target_vcf supports BEDs with overlapping contigs + + cerr << "merging overlapping targets ... " << targets.at(i) << endl; + TARGET_SIZE = tmp_target->merge_overlapping_segments(merged_target); + cerr << "merged size: "<< TARGET_SIZE << endl; + + delete tmp_target; + if(i==0) target = merged_target; + else { + tmp_target = new VCFList(); + cerr << "intersect previous target with " << targets.at(i) << endl; + TARGET_SIZE = target->intersect(merged_target,tmp_target); + cerr << "resulting target size: " << TARGET_SIZE << endl; + delete target; + delete merged_target; + target = tmp_target; + } + } + target->set_positional_info_from_bam(main_bam_file.c_str()); + + cerr << "extract on-target vcf records ... " << endl; + if(varVC!=NULL) { + VCFList * _varVC = new VCFList(); + long vcrecords = target->get_target_vcf(varVC, _varVC); + cerr << "main-vcf records on target .. " << vcrecords << endl; + delete varVC; + varVC = _varVC; + } + if(varTruth!=NULL) { + VCFList * _varTruth = new VCFList(); + long trrecords = target->get_target_vcf(varTruth, _varTruth); + cerr << "truth-vcf records on target .. " << trrecords << endl; + delete varTruth; + varTruth = _varTruth; + } + if(varBg!=NULL) { + VCFList * _varBg = new VCFList(); + long bgrecords = target->get_target_vcf(varBg, _varBg); + cerr << "bg-vcf records on target .. " << bgrecords << endl; + delete varBg; + varBg = _varBg; + } + + if(varFilter!=NULL) { + VCFList * _varFilter = new VCFList(); + long bgrecords = target->get_target_vcf(varFilter, _varFilter); + cerr << "filter-vcf records on target .. " << bgrecords << endl; + delete varFilter; + varFilter = _varFilter; + } + + //if(target != NULL) delete target; + + } // finished target intersection + + // remove Bg from Truth + + bool zygosity_match = !bg_match_criteria.empty() && bg_match_criteria.find("z")!=string::npos; + bool allele_match = zygosity_match || (!bg_match_criteria.empty() && bg_match_criteria.find("a")!=string::npos); + //if ((zygosity_match) and (truth_match_criteria.find("p")!=string::npos)) {zygosity_match = false;} + cerr << "zygosity_match = " << zygosity_match << endl; + cerr << "allele_match = " << allele_match << endl; + varTruth->remove(varBg, zygosity_match, allele_match); + + VCFList *tp = NULL, *fp = NULL, *fn = NULL; + + if(!bg_vcf_file.empty()) { + bool require_zygosity_match = !bg_match_criteria.empty() && bg_match_criteria.find("z")!=string::npos; + bool require_allele_match = require_zygosity_match || (!bg_match_criteria.empty() && bg_match_criteria.find("a")!=string::npos); + + tp = new VCFList(); + fp = new VCFList(); + fn = new VCFList(); + varBg->match(varVC, tp, fn, fp, reference, require_allele_match, require_zygosity_match); + } + + if(!truth_vcf_file.empty()) { + bool require_zygosity_match = !truth_match_criteria.empty() && truth_match_criteria.find("z")!=string::npos; + bool require_allele_match = require_zygosity_match || (!truth_match_criteria.empty() && truth_match_criteria.find("a")!=string::npos); + if(fp!=NULL) { + if(varVC!=NULL) delete varVC; + varVC = new VCFList(*fp); + delete fp; + } + if(tp!=NULL) delete tp; + if(fn!=NULL) delete fn; + tp = new VCFList(); + fn = new VCFList(); + fp = new VCFList(); + + varTruth->match(varVC, tp, fn, fp, reference, require_allele_match, require_zygosity_match); + } + + if(!main_bam_file.empty()) { + cerr << "loading positional info from BAM file " << main_bam_file << endl; + fn->set_positional_info_from_bam(main_bam_file.c_str()); + } else if(fn!=NULL) fn->set_dp_to_zero(); + + bool html = (strcmp(out_format.c_str(),"html") == 0); + + cerr << endl; + cout << "vcfcomp version:"<< full_version_string << (html?"
\n":"\n"); + if(TARGET_SIZE>0) cout << "Final Target Size (bp):"<< TARGET_SIZE << (html?"
\n":"\n"); + cout << "\nReport for main-vcf\n**********************************************************" << endl; + print_stats(target, tp == NULL ? varVC : tp , fp, fn, out_dir, out_pref, out_format, json_file, DP, TARGET_SIZE, full_version_string ); + + // inspecting filtered file + + VCFList *tp_filter = NULL, *fp_filter = NULL, *fn_filter = NULL; + + if(varFilter!=NULL) { + + if(!bg_vcf_file.empty()) { + bool require_zygosity_match = !bg_match_criteria.empty() && bg_match_criteria.find("z")!=string::npos; + bool require_allele_match = require_zygosity_match || (!bg_match_criteria.empty() && bg_match_criteria.find("a")!=string::npos); + + tp_filter = new VCFList(); + fp_filter = new VCFList(); + fn_filter = new VCFList(); + varBg->match(varFilter, tp_filter, fn_filter, fp_filter, reference, require_allele_match, require_zygosity_match); + } + + if(!truth_vcf_file.empty()) { + bool require_zygosity_match = !truth_match_criteria.empty() && truth_match_criteria.find("z")!=string::npos; + bool require_allele_match = require_zygosity_match || (!truth_match_criteria.empty() && truth_match_criteria.find("a")!=string::npos); + + if(fp_filter!=NULL) { + if(varFilter!=NULL) delete varFilter; + varFilter = fp_filter; + } + if(tp_filter!=NULL) delete tp_filter; + if(fn_filter!=NULL) delete fn_filter; + tp_filter = new VCFList(); + fn_filter = new VCFList(); + fp_filter = new VCFList(); + + varTruth->match(varFilter, tp_filter, fn_filter, fp_filter, reference, require_allele_match, require_zygosity_match); + } + + if(!main_bam_file.empty()) { + cerr << "loading positional info from BAM file " << main_bam_file << endl; + fn_filter->set_positional_info_from_bam(main_bam_file.c_str()); + } else if(fn_filter!=NULL) fn_filter->set_dp_to_zero(); + + cout << "\nReport for filter-vcf\n**********************************************************" << endl; + print_stats(target, tp_filter == NULL ? varFilter : tp_filter , fp_filter, fn_filter, out_dir, out_pref + "_filtered", out_format, "", DP, TARGET_SIZE, full_version_string ); + + } + + if(target != NULL) delete target; + if(varBg!=NULL) delete varBg; + if(varTruth!=NULL) delete varTruth; + if(varVC!=NULL) delete varVC; + if(varFilter!=NULL) delete varFilter; + if(fn!=NULL) delete fn; + if(fn_filter!=NULL) delete fn_filter; + + + if(tp_filter!=NULL && !optimize.empty()) { + cout << "\nRunning parameter optimization\n**********************************************************" << endl; + cerr << "Parsing info values..." << endl; + tp->parse_info_values(); + tp_filter->parse_info_values(); + fp->parse_info_values(); + fp_filter->parse_info_values(); + cerr << "Optimizing ...." << endl; + + std::stringstream ss(optimize); + string expres; + while(ss.good()) { + getline( ss, expres, ';'); + if(!expres.empty()) { + std::stringstream aa(expres); + string first = "", second = ""; + if(aa.good()) getline( aa, first, ':'); + if(aa.good()) getline( aa, second, ':'); + + string FLAG="",FLAG2=""; + float _min = 15.0, _step = 5.0, _value = 0.0; + int _nbins = 0; + char comp_operator='<'; + + if(!first.empty()) { + std::stringstream bb(first); + + if(bb.good()) try { + string tmp; + getline(bb, FLAG, ','); + getline(bb, tmp, ','); + _min = atof(tmp.c_str()); + getline(bb, tmp, ','); + _step = atof(tmp.c_str()); + getline(bb, tmp, ','); + _nbins = atoi(tmp.c_str()); + } catch(...) { + FLAG=""; + } + } + + if(!second.empty()) { + std::stringstream bb(first); + + if(bb.good()) try { + string tmp; + getline(bb, FLAG2, ','); + getline(bb, tmp, ','); + comp_operator = tmp[0]!='<'?'>':'<'; + getline(bb, tmp, ','); + _value = atof(tmp.c_str()); + } catch(...) { + FLAG2=""; + } + } + + if(!FLAG.empty()) { + if(!FLAG2.empty()) optimizer(tp,tp_filter,fp,fp_filter, FLAG, _min, _step, _nbins, FLAG2, comp_operator, _value); + else optimizer(tp,tp_filter,fp,fp_filter, FLAG, _min, _step, _nbins); + } + } + } + } + + return 0; + // -- free-up memory, not necessary since program terminates here + if(reference!=NULL) delete reference; + if(tp!=NULL) delete tp; + if(fp!=NULL) delete fp; + if(tp_filter!=NULL) delete tp_filter; + if(fp_filter!=NULL) delete fp_filter; + + + +} + +//----------------------------------------------------- diff --git a/Analysis/bbctools/src/AmpliconRegionStatistics.cpp b/Analysis/bbctools/src/AmpliconRegionStatistics.cpp index 2af50749..af14fde0 100644 --- a/Analysis/bbctools/src/AmpliconRegionStatistics.cpp +++ b/Analysis/bbctools/src/AmpliconRegionStatistics.cpp @@ -154,6 +154,7 @@ void AmpliconRegionStatistics::TrackReadsOnRegion( const BamTools::BamAlignment } // pseudo-randomly choose best region of equivalent best regions TargetRegion *bestRegion = m_regionStack[ clockSeed % numBestRegions ]; + m_lastRegionAssigned = bestRegion; bool e2e_or_cov; if( m_sigFacCoverage ) { int32_t trgLen = bestRegion->trgEnd - bestRegion->trgSrt + 1; diff --git a/Analysis/bbctools/src/BbcCoarse.cpp b/Analysis/bbctools/src/BbcCoarse.cpp index c40d66ff..935dae58 100644 --- a/Analysis/bbctools/src/BbcCoarse.cpp +++ b/Analysis/bbctools/src/BbcCoarse.cpp @@ -327,7 +327,8 @@ bool BbcCoarse::SetReference( const BamTools::RefVector &references ) m_contigBinIndex[i] = references[i].RefLength / m_minorBinSize; if( references[i].RefLength % m_majorBinSize ) ++m_contigBinIndex[i]; } - m_numMajorBins = m_numContigs; + // m_minorPerMajor == 1 is a special case where major substitutes for minor bins + m_numMajorBins = m_contigBinIndex[m_numContigs-1]; } // allocate memory and sizes to be filled out or loaded (unused for V0 or V1 w/ minor contigs) delete [] m_majorBins; @@ -336,7 +337,8 @@ bool BbcCoarse::SetReference( const BamTools::RefVector &references ) free(m_minorBinPack); m_minorBinPack = NULL; if( m_write ) { - // estimate of typical work space: realloc() may be necessary for high coverage references + // sizeof(uint32_t) assumes sparse coverage: average 1 byte per targeted base coverage (or 2 for WGNM) + // realloc() may be necessary for high coverage references, doubling storage as necessary m_minorBinPackSize = m_numMajorBins * m_minorPerMajor * sizeof(uint32_t); // ~47Mb for human m_minorBinPack = (uint8_t *)malloc( m_minorBinPackSize ); } diff --git a/Analysis/bbctools/src/BbcCoarse.h b/Analysis/bbctools/src/BbcCoarse.h index 8b26f7ba..7f5193d7 100644 --- a/Analysis/bbctools/src/BbcCoarse.h +++ b/Analysis/bbctools/src/BbcCoarse.h @@ -136,7 +136,9 @@ class BbcCoarse inline void CheckMinorBinPack( int siz ) { if( m_minorBinPackTail + siz > m_minorBinPackSize ) { - m_minorBinPackSize <<= 1; + // double memory size until enough + while( m_minorBinPackTail + siz > m_minorBinPackSize ) + m_minorBinPackSize <<= 1; m_minorBinPack = (uint8_t *)realloc( m_minorBinPack, m_minorBinPackSize ); } } diff --git a/Analysis/bbctools/src/BbcMain.cpp b/Analysis/bbctools/src/BbcMain.cpp index 790d245d..b1d97645 100644 --- a/Analysis/bbctools/src/BbcMain.cpp +++ b/Analysis/bbctools/src/BbcMain.cpp @@ -20,6 +20,7 @@ using namespace BamTools; #include "BbcView.h" #include "BbcIndex.h" #include "BbcDepth.h" +#include "TrackReads.h" // Number alignments returned using SetRegion() appears incorrect when using closed regions! // It appears to work when using right-open regions but has MASSIVE performance issue. @@ -33,7 +34,7 @@ const int s_initialMinJumpLen = 1000000; const int s_initialMaxReadLen = 1000; // Current version number string for bbctools executable -const uint16_t s_versionNumber = 1003; +const uint16_t s_versionNumber = 1103; // Forward declarations of functions used by main() int bbctools_create( BbcUtils::OptParser &optParser ); @@ -56,7 +57,7 @@ int main( int argc, char* argv[] ) { if( subcmd == "create" ) { subcmdFunc = &bbctools_create; parseString = "A=annotationFields:B=bbc:C=covStats:D=covDepths:E=e2eGap,L=minAlignLength,M=minPcCov;"; - parseString += "P=primerLength,Q=minMAPQ,R=regions:S=sumStats:T=readType:a=autoCreateBamIndex "; + parseString += "O=readOrigin:P=primerLength,Q=minMAPQ,R=regions:S=sumStats:T=readType:a=autoCreateBamIndex "; parseString += "b=onTargetBases c=coarse i=index d=noDups r=onTargetReads s=samdepth u=unique"; maxArgs = 0; } else if( subcmd == "report" ) { @@ -122,6 +123,7 @@ int bbctools_create( BbcUtils::OptParser &optParser ) { string sumstatsFile = optParser.getOptValue("sumStats"); string covstatsFile = optParser.getOptValue("covStats"); + string readOrigFile = optParser.getOptValue("readOrigin"); string readType = optParser.getOptValue("readType"); string covDepths = optParser.getOptValue("covDepths","-"); double minPcCov = optParser.getOptNumber("minPcCov"); @@ -144,12 +146,12 @@ int bbctools_create( BbcUtils::OptParser &optParser ) { bool invertOnTarget = false; // check basic valid argument values and combinations - int numOuts = !bbcfileRoot.empty() + !covstatsFile.empty() + !sumstatsFile.empty(); - int numPipes = (bbcfileRoot == "-") + (covstatsFile == "-") + (sumstatsFile == "-"); + int numOuts = !bbcfileRoot.empty() + !covstatsFile.empty() + !sumstatsFile.empty() + !readOrigFile.empty(); + int numPipes = (bbcfileRoot == "-") + (covstatsFile == "-") + (sumstatsFile == "-") + (readOrigFile == "-"); if( numOuts == 0 && !f_bci && !f_cbc ) { bbcfileRoot = "-"; // default if no other output specified } else if( numPipes > 1 ) { - cerr << "Error: bbctools create: Only one file output (--covStats, --sumStats or --bbc) may be piped to STDOUT." << endl; + cerr << "Error: bbctools create: Only one file output (--covStats, --sumStats, --readOrigin or --bbc) may be piped to STDOUT." << endl; return -1; } else if( samdepth && numOuts ) { cerr << "Error: bbctools create: --samdepth (-s) option may only be used without other output options." << endl; @@ -192,6 +194,10 @@ int bbctools_create( BbcUtils::OptParser &optParser ) { cerr << "Error: --samdepth option is not supported for BBC source files." << endl; return -1; } + if( !readOrigFile.empty() ) { + cerr << "Error: --readOrigin option is not supported for BBC source files." << endl; + return -1; + } } else { // check / open for multiple BAM file inputs if ( !bamReader.Open(cmdArgs) ) { @@ -209,12 +215,17 @@ int bbctools_create( BbcUtils::OptParser &optParser ) { errMsg = BbcUtils::stringTrim(errMsg); errMsg[0] = toupper(errMsg[0]); cerr << endl << errMsg << "." << endl; - return -1; + return 1; } } // grab reference list from either input source const RefVector &references = haveBbcFile ? bbcView.GetReferenceData() : bamReader.GetReferenceData(); - + if( !references.size() ) { + // Issue would already been detected if input was BBC file + cerr << "ERROR: " << (cmdArgs.size() > 1 ? "One or more " : ""); + cerr << "BAM file contains unaligned reads (no references).\n"; + return 1; + } // check/set up target regions input regions/region statistics output RegionCoverage *regions = NULL; string covstatsStaticFields; @@ -312,6 +323,15 @@ int bbctools_create( BbcUtils::OptParser &optParser ) { bbcView.ReadAll(); } } else { + // Test read tracking option for file write + TrackReads *readTracker = NULL; + try { + if( !readOrigFile.empty() ) + readTracker = new TrackReads( readOrigFile, regions ); + } catch( std::runtime_error & ) { + cerr << "ERROR: Unable to write to read tracking file " << readOrigFile << endl; + return 1; + } // BAM reader, BaseCoverage driver, dispatching to BbcCreate and BbcView objects BaseCoverage baseCov(references); baseCov.SetRegionCoverage(regions); @@ -320,9 +340,10 @@ int bbctools_create( BbcUtils::OptParser &optParser ) { if( bbcfileRoot == "-" ) { baseCov.SetBbcView(&bbcView); } + // Certain options require that all reads are processed, invalidating other performance options + bool trackAllReads = !sumstatsFile.empty() || readTracker; // Implicit set of onlyOnTargetReads for performance when only these reads are required bool useBaseCov = (bbcfileRoot == "-" || bbcCreate); - bool trackAllReads = !sumstatsFile.empty(); if( !targetRegions.empty() && !trackAllReads ) { onlyOnTargetReads |= onlyOnTargetBases; if( samdepth || !useBaseCov ) onlyOnTargetReads = true; @@ -398,9 +419,8 @@ int bbctools_create( BbcUtils::OptParser &optParser ) { onlyOnTargetReads = false; if( trackAllReads ) { // force tracking of off-target reads - if( trackAllReads ) { - regions->TrackReadsOnRegion(aln,endPos); - } + regions->TrackReadsOnRegion(aln,endPos); + if( readTracker ) readTracker->Write(aln,endPos); continue; } break; @@ -419,6 +439,7 @@ int bbctools_create( BbcUtils::OptParser &optParser ) { // force tracking of off-target reads if( trackAllReads ) { regions->TrackReadsOnRegion(aln,endPos); + if( readTracker ) readTracker->Write(aln,endPos); } continue; // current is before next target region - fetch the next within bounds } @@ -436,6 +457,9 @@ int bbctools_create( BbcUtils::OptParser &optParser ) { if( regions ) { regions->TrackReadsOnRegion(aln,endPos); } + if( readTracker ) { + readTracker->Write(aln,endPos); + } } // flush and close objects associated with output baseCov.Flush(); @@ -599,7 +623,7 @@ int bbctools_view( BbcUtils::OptParser &optParser ) if( numBins == 0 && binSize == 0 && isolateRegions == false ) { // default view => disallow options dependent on binned-only options if( annotate ) { - cerr << "Error: The --annotationFields (-A) option is only valid for binned coverage (with -B, -N or -i options)." << endl; + cerr << "Error: The --Fields (-A) option is only valid for binned coverage (with -B, -N or -i options)." << endl; return -1; } if( srtBin ) { diff --git a/Analysis/bbctools/src/BbcUsage.h b/Analysis/bbctools/src/BbcUsage.h index 9baf82a8..50c42075 100644 --- a/Analysis/bbctools/src/BbcUsage.h +++ b/Analysis/bbctools/src/BbcUsage.h @@ -49,6 +49,17 @@ static void Usage( string subcmd, bool verbose = false ) { " text file with calculated fields that depend on the --readType and other options specified.\n" " Base and/or read coverage is provided per contig/chromosome, or for each target region if the\n" " --regions (-R) option is provided. A value of '-' may be used to pipe this output to STDOUT.\n" + " -O,--readOrigin File name for read origin output file, which is a tab-delimited text file\n" + " with the assigned target and/or aligned length for every mapped read in the input BAM file.\n" + " The target is identified as the line number of the output --covStats file, or 0 if the read did\n" + " not overlap any target. (It is also the line number for targets defined in the input --regions\n" + " file if this is ordered vs. the reference.) If no --covStats or --regions option is specified the\n" + " output will only have aligned lengths. A value of '-' may be used to pipe this output to STDOUT.\n" + " Usage notes: The association to original read is only made by the line number, which directly\n" + " corresponds to that in the BAM file, or record output by 'samtools view -F 4 '.\n" + " For this reason using a multiple input BAM files is not recommended. (Pre-merge instead.)\n" + " Similarly, if input BAM read filtering options -L, -Q, -d or -u are employed (see below) then\n" + " the SAM read line should be created using equivalent samtools view options.\n" " -R,--regions Input BED file for defining 'on-target' regions flagged in the BBC and\n" " and other coverage files created. A value of '-' may be used to explicitly indicate no target\n" " regions and may be used with an input BBC file to remove on-target information.\n" diff --git a/Analysis/bbctools/src/BbcView.cpp b/Analysis/bbctools/src/BbcView.cpp index a96f1cee..2e66a237 100644 --- a/Analysis/bbctools/src/BbcView.cpp +++ b/Analysis/bbctools/src/BbcView.cpp @@ -559,6 +559,7 @@ bool BbcView::ReadRegion( uint32_t srtContig, uint32_t srtPosition, uint32_t end StreamCoverage( srtPosition++, 0, 0, 0 ); if( srtPosition == endPos ) return true; } + if( srtContig > endContig || m_position >= endPos ) break; } else { if( srtContig > endContig || m_position >= endPos ) break; srtPosition = m_position; diff --git a/Analysis/bbctools/src/RegionCoverage.cpp b/Analysis/bbctools/src/RegionCoverage.cpp index 5840b53e..274c2514 100644 --- a/Analysis/bbctools/src/RegionCoverage.cpp +++ b/Analysis/bbctools/src/RegionCoverage.cpp @@ -20,6 +20,7 @@ RegionCoverage::RegionCoverage( const BamTools::RefVector& references ) : m_contigList(NULL) , m_bcovRegion(NULL) , m_rcovRegion(NULL) + , m_lastRegionAssigned(NULL) , m_numAuxFields(0) , m_ncovDepths(0) { @@ -57,6 +58,7 @@ void RegionCoverage::Clear() m_contigList = NULL; } m_bcovContigIdx = m_rcovContigIdx = m_numRefContigs; + m_lastRegionAssigned = NULL; } uint32_t *RegionCoverage::CreateTargetBinSizes( uint32_t &numBins, uint32_t binSize, @@ -229,6 +231,11 @@ string RegionCoverage::FieldsOnRegion( uint32_t contigIdx, uint32_t readSrt, uin return fieldVals; } +// Return the target index for the region the last call to ReadOnRegion() mapped to, or 0 if none +uint32_t RegionCoverage::GetLastReadOnRegionIdx() const { + return m_lastRegionAssigned ? m_lastRegionAssigned->trgIdx : 0; +} + bool RegionCoverage::GetNextRegion( int &contigIdx, int &srtPosition, int &endPosition, bool start ) { static uint32_t s_contig = 0; @@ -289,19 +296,23 @@ string RegionCoverage::Load( } m_numAuxFields = auxFieldsIdx.size(); vector auxFieldValues(m_numAuxFields,""); + size_t numAuxFields = auxFieldValues.size(); + bool auxWarn = true; ifstream trgfile(fileName.c_str()); if( !trgfile.is_open() ) { return "Failed to open region file "+fileName; } string line, lastContig(""); - int lineNum = 0, numTracks = 0; + int lineNum = 0, numTracks = 0, numFields = 0; + uint32_t srt, end; size_t contigIdx = 0; TargetContig *currentContig = NULL; while( getline(trgfile,line) ) { ++lineNum; vector fields = stringTrimSplit(line,'\t'); - if( fields[0].empty() ) continue; + int fsize = fields.size(); + if( !fsize ) continue; // handle header line - may later depend on fileType if( filetype == 1 && fields[0].substr(0,5) == "track" ) { if( ++numTracks > 1 ) { @@ -318,7 +329,6 @@ string RegionCoverage::Load( continue; } // check primary data fields - int fsize = fields.size(); for( size_t i = 0; i < 3; ++i ) { if( trgFieldsIdx[i] >= fsize || fields[trgFieldsIdx[i]].empty() ) { ostringstream ss; @@ -327,6 +337,16 @@ string RegionCoverage::Load( return ss.str(); } } + // check number of fields consistency + if( numFields != fsize ) { + if( !numFields ) { + numFields = fsize; + } else { + ostringstream ss; + ss << "Inconsistent number of fields in regions file " << fileName << " at line " << lineNum; + return ss.str(); + } + } // check for new contig start string contig = fields[trgFieldsIdx[0]]; if( lastContig != contig ) { @@ -343,8 +363,12 @@ string RegionCoverage::Load( currentContig = m_contigList[contigIdx]; } // get both coordinates to 1-base - uint32_t srt = stringToInteger(fields[trgFieldsIdx[1]]) + srtPosAdj; - uint32_t end = stringToInteger(fields[trgFieldsIdx[2]]); + try { + srt = stringToInteger(fields[trgFieldsIdx[1]]) + srtPosAdj; + end = stringToInteger(fields[trgFieldsIdx[2]]); + } catch( runtime_error &e ) { + return "Format error in regions file "+fileName+": "+e.what(); + } if( srt == end+1 ) { fprintf(stderr,"WARNING: Bed file region has zero length at line %d. Discarded.\n",lineNum); continue; @@ -356,10 +380,16 @@ string RegionCoverage::Load( return ss.str(); } // grab auxiliary fields for new region data - if( auxFieldValues.size() ) { + // - missing auxiliary fields are given value "" with a warning for first occurrence + if( numAuxFields ) { for( size_t i = 0; i < m_numAuxFields; ++i ) { int idx = auxFieldsIdx[i]; if( idx < 0 ) idx += fsize; + if( idx >= fsize && auxWarn ) { + cerr << "Warning: No value found for auxiliary field " << idx << " at line " << lineNum; + cerr << " of regions file " << fileName << ". Missing values will default to empty.\n"; + auxWarn = false; + } auxFieldValues[i] = (idx >= 0 && idx < fsize) ? fields[idx] : ""; } } @@ -367,8 +397,10 @@ string RegionCoverage::Load( } trgfile.close(); // create array of sorted regions for mapping + uint32_t rgnIdx = 0; for( size_t i = 0; i < m_numRefContigs; ++i ) { - m_contigList[i]->MakeSortedArray(); + m_contigList[i]->ReverseSort(rgnIdx); + rgnIdx += m_contigList[i]->numRegions; } return ""; } @@ -451,7 +483,7 @@ void RegionCoverage::SetWholeContigTargets() vector auxFieldValues; for( size_t i = 0; i < m_numRefContigs; ++i ) { m_contigList[i]->AddRegion( new TargetRegion( 1, m_contigList[i]->length, auxFieldValues ) ); - m_contigList[i]->MakeSortedArray(); + m_contigList[i]->ReverseSort(i); } } @@ -495,11 +527,8 @@ void RegionCoverage::Write( const string &filename, const string &columnTitles ) if( haveHeader ) fprintf( fout, "\n" ); } for( size_t i = 0; i < m_numRefContigs; ++i ) { - TargetRegion **targetRegionArray = m_contigList[i]->targetRegionArray; - if( !targetRegionArray ) continue; const char *contig = m_contigList[i]->id.c_str(); - for( size_t j = 0; j < m_contigList[i]->numRegions; ++j ) { - TargetRegion *tr = targetRegionArray[j]; + for( TargetRegion *tr = m_contigList[i]->targetRegionHead; tr; tr = tr->next ) { fprintf( fout, "%s\t%d\t%d", contig, tr->trgSrt, tr->trgEnd ); vector &auxData = tr->auxData; for( size_t k = 0; k < auxData.size(); ++k ) { @@ -643,6 +672,7 @@ uint32_t RegionCoverage::BaseOnRegion( uint32_t contigIdx, uint32_t position ) // Tracks first on-target in instance object so can be iterated over all hit targets. // Assumes reads are given in order (for a single contig) so the search starts where it left off // or at the start of new contig. A reset for the current contig can be forced by using readEnd = 0 +// Side-effect: Record the (reference sorted) index of the overlapped region or 0 if none. uint32_t RegionCoverage::ReadOnRegion( uint32_t contigIdx, uint32_t readSrt, uint32_t readEnd ) { if( contigIdx != m_rcovContigIdx && contigIdx < m_numRefContigs ) { @@ -654,7 +684,11 @@ uint32_t RegionCoverage::ReadOnRegion( uint32_t contigIdx, uint32_t readSrt, uin // here we want the first region overlapped by the read for( ; m_rcovRegion; m_rcovRegion = m_rcovRegion->next ) { if( readEnd < m_rcovRegion->trgSrt ) break; - if( readSrt <= m_rcovRegion->trgEnd ) return 1; + if( readSrt <= m_rcovRegion->trgEnd ) { + m_lastRegionAssigned = m_rcovRegion; + return 1; + } } + m_lastRegionAssigned = NULL; return 0; } diff --git a/Analysis/bbctools/src/RegionCoverage.h b/Analysis/bbctools/src/RegionCoverage.h index 762e7b01..0c09c0d4 100644 --- a/Analysis/bbctools/src/RegionCoverage.h +++ b/Analysis/bbctools/src/RegionCoverage.h @@ -25,6 +25,7 @@ class RegionCoverage protected: struct TargetRegion { + uint32_t trgIdx; uint32_t trgSrt; uint32_t trgEnd; vector auxData; @@ -33,7 +34,8 @@ class RegionCoverage TargetRegion *next; TargetRegion( uint32_t srt, uint32_t end, const vector& aux ) - : trgSrt(srt) + : trgIdx(0) + , trgSrt(srt) , trgEnd(end) , auxData(aux) , extData(NULL) @@ -54,8 +56,7 @@ class RegionCoverage , revReads(0) , fwdTrgReads(0) , revTrgReads(0) - , targetRegionHead(NULL) - , targetRegionArray(NULL) {} + , targetRegionHead(NULL) {} ~TargetContig(void) { TargetRegion *nrg = targetRegionHead; @@ -64,7 +65,6 @@ class RegionCoverage delete nrg; nrg = next; } - delete [] targetRegionArray; } // Resulting link list starting at targetRegionHead will be in reverse order of target regions. @@ -72,7 +72,7 @@ class RegionCoverage // reverse order f to top of the list given pre-ordered target regions. void AddRegion( TargetRegion *nrg ) { if( !nrg ) return; - ++numRegions; + nrg->trgIdx = ++numRegions; // initially set to order loaded if( !targetRegionHead ) { targetRegionHead = nrg; return; @@ -95,37 +95,27 @@ class RegionCoverage nrg->next = cur; } - // convert linked list into array and forward list for quick access - void MakeSortedArray(void) { - delete targetRegionArray; - if( !numRegions ) { - targetRegionArray = NULL; - return; - } - targetRegionArray = new TargetRegion*[numRegions]; - TargetRegion *cur = targetRegionHead; - int lastReg = (int)numRegions-1; - for( int i = lastReg; i >= 0; --i ) { - targetRegionArray[i] = cur; - cur = cur->next; + // convert reverse ordered linked list to be forward ordered and reset region index + void ReverseSort( uint32_t idxOffset = 0 ) { + TargetRegion *prv = NULL, *nxt; + idxOffset += numRegions; + for( TargetRegion *cur = targetRegionHead; cur; cur = nxt ) { + cur->trgIdx = idxOffset--; + nxt = cur->next; + cur->next = prv; + prv = cur; } - // reverse order of linked list so this may be used in addition to array - targetRegionHead = cur = targetRegionArray[0]; - for( int i = 0; i < lastReg; ++i ) { - cur = cur->next = targetRegionArray[i+1]; - } - cur->next = NULL; + targetRegionHead = prv; } string id; int32_t length; - size_t numRegions; + uint32_t numRegions; uint64_t fwdReads; uint64_t revReads; uint64_t fwdTrgReads; uint64_t revTrgReads; TargetRegion *targetRegionHead; - TargetRegion **targetRegionArray; }; size_t m_numRefContigs; @@ -140,6 +130,7 @@ class RegionCoverage TargetRegion *m_bcovRegion; uint32_t m_rcovContigIdx; TargetRegion *m_rcovRegion; + TargetRegion *m_lastRegionAssigned; // common optional depth at coverage stats size_t m_numAuxFields; @@ -169,6 +160,9 @@ class RegionCoverage // the given locus. If more than maxValues then return ,...(N)..., (N>1). string FieldsOnRegion( uint32_t contigIdx, uint32_t readSrt, uint32_t readEnd, uint32_t maxValues = 3 ); + // Return the target index for the region the last call to ReadOnRegion() mapped to, or 0 if none + uint32_t GetLastReadOnRegionIdx(void) const; + // An iterator of all target regions returning false for a call after the last region is returned. // The optional start argument may be used to reset this iterator to the first region. // Note that srtPositon is returned as a 0-based integer (etc.) for intended usage with the BamMultiReader::SetRegion(). diff --git a/Analysis/bbctools/src/TrackReads.cpp b/Analysis/bbctools/src/TrackReads.cpp new file mode 100644 index 00000000..4cfbd320 --- /dev/null +++ b/Analysis/bbctools/src/TrackReads.cpp @@ -0,0 +1,39 @@ +// Copyright (C) 2016 Thermo Fisher Scientific. All Rights Reserved. +/* + * TrackReads.cpp + * + * Created on: Sep 8, 2016 + * Author: Guy Del Mistro + */ + +#include "TrackReads.h" + +TrackReads::TrackReads( const string& filename, const RegionCoverage* regions ) + : m_filename(filename) + , m_regions(regions) + , m_outfile(NULL) +{ + if( !filename.empty() && filename != "-" ) { + m_outfile = fopen( m_filename.c_str(), "w" ); + if( !m_outfile ) throw std::runtime_error("TrackReads(): Failed to open file for write: "+m_filename); + } +} + +TrackReads::~TrackReads() +{ + if( m_outfile ) fclose(m_outfile); +} + +void TrackReads::Write( const BamTools::BamAlignment &aread, uint32_t endPos ) +{ + uint32_t rend = endPos ? endPos : aread.GetEndPosition(); + uint32_t rlen = rend - aread.Position; + FILE *out = m_outfile ? m_outfile : stdout; + if( m_regions ) { + fprintf( out, "%u\t%u\n",m_regions->GetLastReadOnRegionIdx(),rlen); + } else { + // Default output if no target regions resource was provided + fprintf( out, "%u\n",rlen); + } +} + diff --git a/Analysis/bbctools/src/TrackReads.h b/Analysis/bbctools/src/TrackReads.h new file mode 100644 index 00000000..6d25ee1a --- /dev/null +++ b/Analysis/bbctools/src/TrackReads.h @@ -0,0 +1,31 @@ +// Copyright (C) 2016 Thermo Fisher Scientific. All Rights Reserved. +/* + * TrackReads.h + * + * Created on: Sep 8, 2016 + * Author: Guy Del Mistro + */ + +// The TrackReads class is defined with access to the BAM read and target region data +// so that this read tracking output can be expanded on later. + +#ifndef TRACKREADS_H_ +#define TRACKREADS_H_ + +#include "RegionCoverage.h" + +class TrackReads +{ + public: + TrackReads( const string& filename, const RegionCoverage* regions = NULL ); + virtual ~TrackReads(void); + + void Write( const BamTools::BamAlignment &aread, uint32_t endPos ); + + private: + const string& m_filename; + const RegionCoverage* m_regions; + FILE *m_outfile; +}; + +#endif /* TRACKREADS_H_ */ diff --git a/Analysis/config/args_314_beadfind.json b/Analysis/config/args_314_beadfind.json index ea5c73c6..b8ea78b7 100644 --- a/Analysis/config/args_314_beadfind.json +++ b/Analysis/config/args_314_beadfind.json @@ -11,6 +11,8 @@ "beadfind-lib-filt" : 1.0, "beadfind-lib-min-peak" : 10.0, "beadfind-mesh-step" : [], + "beadfind-acq-threshold" : [ -10, 3, 5, 500 ], + "beadfind-bf-threshold" : [ -5, 300, -20000, -10 ], "beadfind-min-tf-snr" : 7.0, "beadfind-minlive" : 0.00010, "beadfind-minlivesnr" : 4.0, @@ -32,7 +34,8 @@ "bfold" : true, "datacollect-gain-correction" : false, "noduds" : false, - "use-beadmask" : "" + "use-beadmask" : "", + "exclusion-mask" : "exclusionMask_314.bin" }, "DebugMe" : { "bkg-dbg-trace" : [], diff --git a/Analysis/config/args_316_beadfind.json b/Analysis/config/args_316_beadfind.json index 0609b4ba..f40a9cf6 100644 --- a/Analysis/config/args_316_beadfind.json +++ b/Analysis/config/args_316_beadfind.json @@ -11,6 +11,8 @@ "beadfind-lib-filt" : 1.0, "beadfind-lib-min-peak" : 10.0, "beadfind-mesh-step" : [], + "beadfind-acq-threshold" : [ -10, 3, 5, 500 ], + "beadfind-bf-threshold" : [ -5, 300, -20000, -10 ], "beadfind-min-tf-snr" : 7.0, "beadfind-minlive" : 0.00010, "beadfind-minlivesnr" : 4.0, @@ -32,7 +34,8 @@ "bfold" : true, "datacollect-gain-correction" : false, "noduds" : false, - "use-beadmask" : "" + "use-beadmask" : "", + "exclusion-mask" : "exclusionMask_316.bin" }, "DebugMe" : { "bkg-dbg-trace" : [], diff --git a/Analysis/config/args_316v2_beadfind.json b/Analysis/config/args_316v2_beadfind.json index 3b9baf23..375774af 100644 --- a/Analysis/config/args_316v2_beadfind.json +++ b/Analysis/config/args_316v2_beadfind.json @@ -11,6 +11,8 @@ "beadfind-lib-filt" : 1.0, "beadfind-lib-min-peak" : 10.0, "beadfind-mesh-step" : [], + "beadfind-acq-threshold" : [ -10, 3, 5, 500 ], + "beadfind-bf-threshold" : [ -5, 300, -20000, -10 ], "beadfind-min-tf-snr" : 7.0, "beadfind-minlive" : 0.00010, "beadfind-minlivesnr" : 4.0, @@ -32,7 +34,8 @@ "bfold" : true, "datacollect-gain-correction" : false, "noduds" : false, - "use-beadmask" : "" + "use-beadmask" : "", + "exclusion-mask" : "exclusionMask_316v2.bin" }, "DebugMe" : { "bkg-dbg-trace" : [], diff --git a/Analysis/config/args_318D_beadfind.json b/Analysis/config/args_318D_beadfind.json index 59fadcb6..091d95b7 100644 --- a/Analysis/config/args_318D_beadfind.json +++ b/Analysis/config/args_318D_beadfind.json @@ -11,6 +11,8 @@ "beadfind-lib-filt" : 1.0, "beadfind-lib-min-peak" : 10.0, "beadfind-mesh-step" : [], + "beadfind-acq-threshold" : [ -10, 3, 5, 500 ], + "beadfind-bf-threshold" : [ -5, 300, -20000, -10 ], "beadfind-min-tf-snr" : 7.0, "beadfind-minlive" : 0.00010, "beadfind-minlivesnr" : 4.0, @@ -32,7 +34,8 @@ "bfold" : true, "datacollect-gain-correction" : false, "noduds" : false, - "use-beadmask" : "" + "use-beadmask" : "", + "exclusion-mask" : "exclusionMask_318.bin" }, "DebugMe" : { "bkg-dbg-trace" : [], diff --git a/Analysis/config/args_318_beadfind.json b/Analysis/config/args_318_beadfind.json index f00751bd..0ccfa267 100644 --- a/Analysis/config/args_318_beadfind.json +++ b/Analysis/config/args_318_beadfind.json @@ -11,6 +11,8 @@ "beadfind-lib-filt" : 1.0, "beadfind-lib-min-peak" : 10.0, "beadfind-mesh-step" : [], + "beadfind-acq-threshold" : [ -10, 3, 5, 500 ], + "beadfind-bf-threshold" : [ -5, 300, -20000, -10 ], "beadfind-min-tf-snr" : 7.0, "beadfind-minlive" : 0.00010, "beadfind-minlivesnr" : 4.0, @@ -32,7 +34,8 @@ "bfold" : true, "datacollect-gain-correction" : false, "noduds" : false, - "use-beadmask" : "" + "use-beadmask" : "", + "exclusion-mask" : "exclusionMask_318.bin" }, "DebugMe" : { "bkg-dbg-trace" : [], diff --git a/Analysis/config/args_318select_beadfind.json b/Analysis/config/args_318select_beadfind.json index c994637c..34b2b91e 100644 --- a/Analysis/config/args_318select_beadfind.json +++ b/Analysis/config/args_318select_beadfind.json @@ -11,6 +11,8 @@ "beadfind-lib-filt" : 1.0, "beadfind-lib-min-peak" : 10.0, "beadfind-mesh-step" : [], + "beadfind-acq-threshold" : [ -10, 3, 5, 500 ], + "beadfind-bf-threshold" : [ -5, 300, -20000, -10 ], "beadfind-min-tf-snr" : 7.0, "beadfind-minlive" : 0.00010, "beadfind-minlivesnr" : 4.0, diff --git a/Analysis/config/args_520_beadfind.json b/Analysis/config/args_520_beadfind.json index 2e0298dc..ec6b2dbb 100644 --- a/Analysis/config/args_520_beadfind.json +++ b/Analysis/config/args_520_beadfind.json @@ -11,6 +11,8 @@ "beadfind-lib-filt" : 1.0, "beadfind-lib-min-peak" : 10.0, "beadfind-mesh-step" : [ 80, 112 ], + "beadfind-acq-threshold" : [ -10, 10, 5, 500 ], + "beadfind-bf-threshold" : [ -5, 300, -20000, -10 ], "beadfind-min-tf-snr" : 4.0, "beadfind-minlive" : 0.00010, "beadfind-minlivesnr" : 3.0, @@ -31,7 +33,8 @@ "bfold" : true, "datacollect-gain-correction" : true, "noduds" : false, - "use-beadmask" : "" + "use-beadmask" : "", + "exclusion-mask" : "exclusionMask_520.txt" }, "DebugMe" : { "bkg-dbg-trace" : [], diff --git a/Analysis/config/args_521_beadfind.json b/Analysis/config/args_521_beadfind.json index db1e7f0a..b04c4fc6 100644 --- a/Analysis/config/args_521_beadfind.json +++ b/Analysis/config/args_521_beadfind.json @@ -11,6 +11,8 @@ "beadfind-lib-filt" : 1.0, "beadfind-lib-min-peak" : 10.0, "beadfind-mesh-step" : [ 80, 112 ], + "beadfind-acq-threshold" : [ -10, 10, 5, 500 ], + "beadfind-bf-threshold" : [ -5, 300, -20000, -10 ], "beadfind-min-tf-snr" : 4.0, "beadfind-minlive" : 0.00010, "beadfind-minlivesnr" : 3.0, @@ -31,7 +33,8 @@ "bfold" : true, "datacollect-gain-correction" : true, "noduds" : false, - "use-beadmask" : "" + "use-beadmask" : "", + "exclusion-mask" : "exclusionMask_521.txt" }, "DebugMe" : { "bkg-dbg-trace" : [], diff --git a/Analysis/config/args_522_beadfind.json b/Analysis/config/args_522_beadfind.json index 51b10819..65d3ddcd 100644 --- a/Analysis/config/args_522_beadfind.json +++ b/Analysis/config/args_522_beadfind.json @@ -11,6 +11,8 @@ "beadfind-lib-filt" : 1.0, "beadfind-lib-min-peak" : 10.0, "beadfind-mesh-step" : [ 80, 112 ], + "beadfind-acq-threshold" : [ -10, 10, 5, 500 ], + "beadfind-bf-threshold" : [ -5, 300, -20000, -10 ], "beadfind-min-tf-snr" : 4.0, "beadfind-minlive" : 0.00010, "beadfind-minlivesnr" : 3.0, @@ -31,7 +33,8 @@ "bfold" : true, "datacollect-gain-correction" : true, "noduds" : false, - "use-beadmask" : "" + "use-beadmask" : "", + "exclusion-mask" : "exclusionMask_522.txt" }, "DebugMe" : { "bkg-dbg-trace" : [], diff --git a/Analysis/config/args_530_beadfind.json b/Analysis/config/args_530_beadfind.json index 91177ecb..08b72012 100644 --- a/Analysis/config/args_530_beadfind.json +++ b/Analysis/config/args_530_beadfind.json @@ -11,6 +11,8 @@ "beadfind-lib-filt" : 1.0, "beadfind-lib-min-peak" : 10.0, "beadfind-mesh-step" : [ 80, 112 ], + "beadfind-acq-threshold" : [ -10, 10, 5, 500 ], + "beadfind-bf-threshold" : [ -5, 300, -20000, -10 ], "beadfind-min-tf-snr" : 4.0, "beadfind-minlive" : 0.00010, "beadfind-minlivesnr" : 3.0, @@ -31,7 +33,8 @@ "bfold" : true, "datacollect-gain-correction" : true, "noduds" : false, - "use-beadmask" : "" + "use-beadmask" : "", + "exclusion-mask" : "exclusionMask_530.txt" }, "DebugMe" : { "bkg-dbg-trace" : [], diff --git a/Analysis/config/args_540_beadfind.json b/Analysis/config/args_540_beadfind.json index 86536178..95cd65f0 100644 --- a/Analysis/config/args_540_beadfind.json +++ b/Analysis/config/args_540_beadfind.json @@ -11,6 +11,8 @@ "beadfind-lib-filt" : 1.0, "beadfind-lib-min-peak" : 10.0, "beadfind-mesh-step" : [ 108, 112 ], + "beadfind-acq-threshold" : [ -10, 10, 5, 500 ], + "beadfind-bf-threshold" : [ -5, 300, -20000, -10 ], "beadfind-min-tf-snr" : 4.0, "beadfind-minlive" : 0.00010, "beadfind-minlivesnr" : 3.0, @@ -31,7 +33,8 @@ "bfold" : true, "datacollect-gain-correction" : true, "noduds" : false, - "use-beadmask" : "" + "use-beadmask" : "", + "exclusion-mask" : "exclusionMask_540.txt" }, "DebugMe" : { "bkg-dbg-trace" : [], diff --git a/Analysis/config/args_541_beadfind.json b/Analysis/config/args_541_beadfind.json index b2c77b66..7f14e4ca 100644 --- a/Analysis/config/args_541_beadfind.json +++ b/Analysis/config/args_541_beadfind.json @@ -11,6 +11,8 @@ "beadfind-lib-filt" : 1.0, "beadfind-lib-min-peak" : 10.0, "beadfind-mesh-step" : [ 108, 112 ], + "beadfind-acq-threshold" : [ -10, 10, 5, 500 ], + "beadfind-bf-threshold" : [ -5, 300, -20000, -10 ], "beadfind-min-tf-snr" : 4.0, "beadfind-minlive" : 0.00010, "beadfind-minlivesnr" : 3.0, @@ -31,7 +33,8 @@ "bfold" : true, "datacollect-gain-correction" : true, "noduds" : false, - "use-beadmask" : "" + "use-beadmask" : "", + "exclusion-mask" : "exclusionMask_541.txt" }, "DebugMe" : { "bkg-dbg-trace" : [], diff --git a/Analysis/config/args_550_analysis.json b/Analysis/config/args_550_analysis.json index f2c87e80..c2e8a03f 100644 --- a/Analysis/config/args_550_analysis.json +++ b/Analysis/config/args_550_analysis.json @@ -39,7 +39,7 @@ "GlobalDefaultsForBkgModel" : { "barcode-spec-file" : "", "bkg-dont-emphasize-by-compression" : false, - "bkg-well-xtalk-name" : "/opt/ion/config/xtalk.p2.2.1.settings.20140120.json", + "bkg-well-xtalk-name" : "/opt/ion/config/xtalk.550.well.settings.json", "gopt" : "/opt/ion/config/gopt_550.param.json", "xtalk" : "disable" }, diff --git a/Analysis/config/args_550_beadfind.json b/Analysis/config/args_550_beadfind.json index e0dab861..07c39cdb 100644 --- a/Analysis/config/args_550_beadfind.json +++ b/Analysis/config/args_550_beadfind.json @@ -11,6 +11,8 @@ "beadfind-lib-filt" : 1.0, "beadfind-lib-min-peak" : 10.0, "beadfind-mesh-step" : [ 108, 112 ], + "beadfind-acq-threshold" : [ -10, 10, 5, 500 ], + "beadfind-bf-threshold" : [ -5, 300, -20000, -10 ], "beadfind-min-tf-snr" : 4.0, "beadfind-minlive" : 0.00010, "beadfind-minlivesnr" : 3.0, @@ -31,7 +33,8 @@ "bfold" : true, "datacollect-gain-correction" : true, "noduds" : false, - "use-beadmask" : "" + "use-beadmask" : "", + "exclusion-mask" : "exclusionMask_550.txt" }, "DebugMe" : { "bkg-dbg-trace" : [], diff --git a/Analysis/config/args_560_beadfind.json b/Analysis/config/args_560_beadfind.json index 8834ac79..00491c0c 100644 --- a/Analysis/config/args_560_beadfind.json +++ b/Analysis/config/args_560_beadfind.json @@ -11,6 +11,8 @@ "beadfind-lib-filt" : 1.0, "beadfind-lib-min-peak" : 10.0, "beadfind-mesh-step" : [ 108, 112 ], + "beadfind-acq-threshold" : [ -10, 10, 5, 500 ], + "beadfind-bf-threshold" : [ -5, 300, -20000, -10 ], "beadfind-min-tf-snr" : 4.0, "beadfind-minlive" : 0.00010, "beadfind-minlivesnr" : 3.0, @@ -31,7 +33,8 @@ "bfold" : true, "datacollect-gain-correction" : true, "noduds" : false, - "use-beadmask" : "" + "use-beadmask" : "", + "exclusion-mask" : "exclusionMask_560.txt" }, "DebugMe" : { "bkg-dbg-trace" : [], diff --git a/Analysis/config/args_900_beadfind.json b/Analysis/config/args_900_beadfind.json index 281d9398..505b4951 100644 --- a/Analysis/config/args_900_beadfind.json +++ b/Analysis/config/args_900_beadfind.json @@ -11,6 +11,8 @@ "beadfind-lib-filt" : 1.0, "beadfind-lib-min-peak" : 10.0, "beadfind-mesh-step" : [ 108, 112 ], + "beadfind-acq-threshold" : [ -10, 3, 5, 500 ], + "beadfind-bf-threshold" : [ -5, 300, -20000, -10 ], "beadfind-min-tf-snr" : 4.0, "beadfind-minlive" : 0.00010, "beadfind-minlivesnr" : 3.0, @@ -31,7 +33,8 @@ "bfold" : true, "datacollect-gain-correction" : false, "noduds" : false, - "use-beadmask" : "" + "use-beadmask" : "", + "exclusion-mask" : "exclusionMask_900.txt" }, "DebugMe" : { "bkg-dbg-trace" : [], diff --git a/Analysis/config/args_P1.0.19_beadfind.json b/Analysis/config/args_P1.0.19_beadfind.json index 9ebb525e..76a57eec 100644 --- a/Analysis/config/args_P1.0.19_beadfind.json +++ b/Analysis/config/args_P1.0.19_beadfind.json @@ -11,6 +11,8 @@ "beadfind-lib-filt" : 1.0, "beadfind-lib-min-peak" : 10.0, "beadfind-mesh-step" : [ 80, 112 ], + "beadfind-acq-threshold" : [ -10, 3, 5, 500 ], + "beadfind-bf-threshold" : [ -5, 300, -20000, -10 ], "beadfind-min-tf-snr" : 4.0, "beadfind-minlive" : 0.00010, "beadfind-minlivesnr" : 3.0, @@ -31,7 +33,8 @@ "bfold" : true, "datacollect-gain-correction" : false, "noduds" : false, - "use-beadmask" : "" + "use-beadmask" : "", + "exclusion-mask" : "exclusionMask_p1.0.19.txt" }, "DebugMe" : { "bkg-dbg-trace" : [], diff --git a/Analysis/config/args_P1.0.20_beadfind.json b/Analysis/config/args_P1.0.20_beadfind.json index 987fa6d0..108d961d 100644 --- a/Analysis/config/args_P1.0.20_beadfind.json +++ b/Analysis/config/args_P1.0.20_beadfind.json @@ -11,6 +11,8 @@ "beadfind-lib-filt" : 1.0, "beadfind-lib-min-peak" : 10.0, "beadfind-mesh-step" : [ 80, 112 ], + "beadfind-acq-threshold" : [ -10, 3, 5, 500 ], + "beadfind-bf-threshold" : [ -5, 300, -20000, -10 ], "beadfind-min-tf-snr" : 4.0, "beadfind-minlive" : 0.00010, "beadfind-minlivesnr" : 3.0, @@ -31,7 +33,8 @@ "bfold" : true, "datacollect-gain-correction" : false, "noduds" : false, - "use-beadmask" : "" + "use-beadmask" : "", + "exclusion-mask" : "exclusionMask_p1.0.20.txt" }, "DebugMe" : { "bkg-dbg-trace" : [], diff --git a/Analysis/config/args_P1.1.17_beadfind.json b/Analysis/config/args_P1.1.17_beadfind.json index 24a32856..c6e32dbc 100644 --- a/Analysis/config/args_P1.1.17_beadfind.json +++ b/Analysis/config/args_P1.1.17_beadfind.json @@ -11,6 +11,8 @@ "beadfind-lib-filt" : 1.0, "beadfind-lib-min-peak" : 10.0, "beadfind-mesh-step" : [ 108, 112 ], + "beadfind-acq-threshold" : [ -10, 3, 5, 500 ], + "beadfind-bf-threshold" : [ -5, 300, -20000, -10 ], "beadfind-min-tf-snr" : 4.0, "beadfind-minlive" : 0.00010, "beadfind-minlivesnr" : 3.0, @@ -31,7 +33,8 @@ "bfold" : true, "datacollect-gain-correction" : false, "noduds" : false, - "use-beadmask" : "" + "use-beadmask" : "", + "exclusion-mask" : "exclusionMask_p1.1.17.txt" }, "DebugMe" : { "bkg-dbg-trace" : [], diff --git a/Analysis/config/args_P1.1.541_beadfind.json b/Analysis/config/args_P1.1.541_beadfind.json index ef5c2745..c241619b 100644 --- a/Analysis/config/args_P1.1.541_beadfind.json +++ b/Analysis/config/args_P1.1.541_beadfind.json @@ -11,6 +11,8 @@ "beadfind-lib-filt" : 1.0, "beadfind-lib-min-peak" : 10.0, "beadfind-mesh-step" : [ 108, 112 ], + "beadfind-acq-threshold" : [ -10, 3, 5, 500 ], + "beadfind-bf-threshold" : [ -5, 300, -20000, -10 ], "beadfind-min-tf-snr" : 4.0, "beadfind-minlive" : 0.00010, "beadfind-minlivesnr" : 3.0, diff --git a/Analysis/config/args_P1.2.18_beadfind.json b/Analysis/config/args_P1.2.18_beadfind.json index e599586c..a31d2a54 100644 --- a/Analysis/config/args_P1.2.18_beadfind.json +++ b/Analysis/config/args_P1.2.18_beadfind.json @@ -11,6 +11,8 @@ "beadfind-lib-filt" : 1.0, "beadfind-lib-min-peak" : 10.0, "beadfind-mesh-step" : [ 108, 112 ], + "beadfind-acq-threshold" : [ -10, 3, 5, 500 ], + "beadfind-bf-threshold" : [ -5, 300, -20000, -10 ], "beadfind-min-tf-snr" : 4.0, "beadfind-minlive" : 0.00010, "beadfind-minlivesnr" : 3.0, diff --git a/Analysis/config/args_P2.0.1_beadfind.json b/Analysis/config/args_P2.0.1_beadfind.json index 9bc3bb30..628d1f77 100644 --- a/Analysis/config/args_P2.0.1_beadfind.json +++ b/Analysis/config/args_P2.0.1_beadfind.json @@ -11,6 +11,8 @@ "beadfind-lib-filt" : 1.0, "beadfind-lib-min-peak" : 10.0, "beadfind-mesh-step" : [ 80, 112 ], + "beadfind-acq-threshold" : [ -10, 3, 5, 500 ], + "beadfind-bf-threshold" : [ -5, 300, -20000, -10 ], "beadfind-min-tf-snr" : 4.0, "beadfind-minlive" : 0.00010, "beadfind-minlivesnr" : 3.0, diff --git a/Analysis/config/args_P2.1.1_beadfind.json b/Analysis/config/args_P2.1.1_beadfind.json index d7d03fac..3dbd2e8c 100644 --- a/Analysis/config/args_P2.1.1_beadfind.json +++ b/Analysis/config/args_P2.1.1_beadfind.json @@ -11,6 +11,8 @@ "beadfind-lib-filt" : 1.0, "beadfind-lib-min-peak" : 10.0, "beadfind-mesh-step" : [ 108, 112 ], + "beadfind-acq-threshold" : [ -10, 3, 5, 500 ], + "beadfind-bf-threshold" : [ -5, 300, -20000, -10 ], "beadfind-min-tf-snr" : 4.0, "beadfind-minlive" : 0.00010, "beadfind-minlivesnr" : 3.0, diff --git a/Analysis/config/args_P2.2.1_beadfind.json b/Analysis/config/args_P2.2.1_beadfind.json index c07e1838..047a0adc 100644 --- a/Analysis/config/args_P2.2.1_beadfind.json +++ b/Analysis/config/args_P2.2.1_beadfind.json @@ -11,6 +11,8 @@ "beadfind-lib-filt" : 1.0, "beadfind-lib-min-peak" : 10.0, "beadfind-mesh-step" : [ 108, 112 ], + "beadfind-acq-threshold" : [ -10, 3, 5, 500 ], + "beadfind-bf-threshold" : [ -5, 300, -20000, -10 ], "beadfind-min-tf-snr" : 4.0, "beadfind-minlive" : 0.00010, "beadfind-minlivesnr" : 3.0, diff --git a/Analysis/config/args_P2.2.2_beadfind.json b/Analysis/config/args_P2.2.2_beadfind.json index 17d24534..49a8808f 100644 --- a/Analysis/config/args_P2.2.2_beadfind.json +++ b/Analysis/config/args_P2.2.2_beadfind.json @@ -11,6 +11,8 @@ "beadfind-lib-filt" : 1.0, "beadfind-lib-min-peak" : 10.0, "beadfind-mesh-step" : [ 108, 112 ], + "beadfind-acq-threshold" : [ -10, 3, 5, 500 ], + "beadfind-bf-threshold" : [ -5, 300, -20000, -10 ], "beadfind-min-tf-snr" : 4.0, "beadfind-minlive" : 0.00010, "beadfind-minlivesnr" : 3.0, @@ -31,7 +33,8 @@ "bfold" : true, "datacollect-gain-correction" : false, "noduds" : false, - "use-beadmask" : "" + "use-beadmask" : "", + "exclusion-mask" : "exclusionMask_PQ.txt" }, "DebugMe" : { "bkg-dbg-trace" : [], diff --git a/Analysis/config/args_P2.3.1_analysis.json b/Analysis/config/args_P2.3.1_analysis.json index ac10a548..d6549abc 100644 --- a/Analysis/config/args_P2.3.1_analysis.json +++ b/Analysis/config/args_P2.3.1_analysis.json @@ -39,7 +39,7 @@ "GlobalDefaultsForBkgModel" : { "barcode-spec-file" : "", "bkg-dont-emphasize-by-compression" : false, - "bkg-well-xtalk-name" : "/opt/ion/config/xtalk.p2.2.1.settings.20140120.json", + "bkg-well-xtalk-name" : "/opt/ion/config/xtalk.p2.3.1.well.settings.json", "gopt" : "/opt/ion/config/gopt_p1.1.17_ampliseq_exome.param.json", "xtalk" : "disable" }, @@ -72,7 +72,7 @@ "col-flicker-correct" : true, "col-flicker-correct-aggressive" : true, "col-flicker-correct-verbose" : false, - "corr-noise-correct" : true, + "corr-noise-correct" : false, "dat-postfix" : "dat", "flowtimeoffset" : 1000, "fluid-potential-correct" : false, @@ -190,6 +190,9 @@ "wells-save-queue-size" : 0 }, "ThumbnailControl" : { + "bkg-debug-files" : true, + "bkg-debug-param" : 3, + "gpuworkload" : 0.0, "mask-datacollect-exclude-regions" : false, "region-size" : [ 100, 100 ], "total-timeout" : 36000 diff --git a/Analysis/config/args_P2.3.1_beadfind.json b/Analysis/config/args_P2.3.1_beadfind.json index 25f8f1c5..6feb483e 100644 --- a/Analysis/config/args_P2.3.1_beadfind.json +++ b/Analysis/config/args_P2.3.1_beadfind.json @@ -11,6 +11,8 @@ "beadfind-lib-filt" : 1.0, "beadfind-lib-min-peak" : 10.0, "beadfind-mesh-step" : [ 108, 112 ], + "beadfind-acq-threshold" : [ -10, 3, 5, 500 ], + "beadfind-bf-threshold" : [ -5, 300, -20000, -10 ], "beadfind-min-tf-snr" : 4.0, "beadfind-minlive" : 0.00010, "beadfind-minlivesnr" : 3.0, @@ -31,7 +33,8 @@ "bfold" : true, "datacollect-gain-correction" : false, "noduds" : false, - "use-beadmask" : "" + "use-beadmask" : "", + "exclusion-mask" : "exclusionMask_550.txt" }, "DebugMe" : { "bkg-dbg-trace" : [], @@ -56,7 +59,7 @@ "col-flicker-correct" : true, "col-flicker-correct-aggressive" : true, "col-flicker-correct-verbose" : false, - "corr-noise-correct" : true, + "corr-noise-correct" : false, "dat-postfix" : "dat", "flowtimeoffset" : 1000, "fluid-potential-correct" : false, diff --git a/Analysis/config/exclusionMask_510.txt b/Analysis/config/exclusionMask_510.txt new file mode 100644 index 00000000..585d9601 --- /dev/null +++ b/Analysis/config/exclusionMask_510.txt @@ -0,0 +1,1729 @@ +7680 1728 +398 7288 +397 7288 +397 7289 +396 7290 +396 7290 +395 7291 +395 7291 +394 7292 +394 7293 +393 7293 +393 7294 +392 7294 +391 7295 +391 7296 +390 7296 +390 7297 +389 7298 +388 7298 +388 7299 +387 7300 +387 7300 +386 7301 +385 7301 +385 7302 +384 7303 +384 7303 +383 7304 +382 7305 +382 7305 +381 7306 +380 7307 +380 7307 +379 7308 +379 7309 +378 7309 +377 7310 +377 7311 +376 7311 +375 7312 +375 7312 +374 7313 +373 7314 +373 7314 +372 7315 +371 7316 +371 7316 +370 7317 +369 7318 +369 7318 +368 7319 +367 7320 +367 7320 +366 7321 +365 7322 +365 7322 +364 7323 +363 7324 +363 7324 +362 7325 +361 7326 +361 7326 +360 7327 +359 7327 +359 7328 +358 7329 +357 7329 +357 7330 +356 7331 +355 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b/Analysis/config/gopt_318D.param.json @@ -0,0 +1,64 @@ +{ + "metadata": { + "compatible_chip_type": "318D", + "created": "2016-05-24 15:50:57.069486", + "optimizable params": "km_const(1-4), krate(6-9), d_coeff(11-14), sigma_mult(16-19), t_mid_nuc_delay(21-24), sens(25), tau_R_m(26), tau_R_o(27), emphasis(28-35), emp_amplitude(36), emp_width(37), clonal_call_scale(38-42), tau_E(43), min_tauB/max_tauB/mid_tauB(44/45/46)", + "source": "gopt", + "version": 1 + }, + "parameters": { + "clonal_call_scale": [ + 0.001, + 0.709681, + 0.246037, + 0.02, + 0.11484 + ], + "d_coeff": [ + 26.8412, + 156.299, + 150.531, + 52.4835 + ], + "emp_amplitude": 7.572, + "emp_width": 4.961651, + "emphasis": [ + 7.238, + 1.58, + 1.695, + 1.4475, + 7.3275, + 1.11375, + 0.495, + 11.56725 + ], + "km_const": [ + 18.7799, + 22.2851, + 17.3087, + 15.5264 + ], + "krate": [ + 17.1972, + 25.0697, + 25.2226, + 31.6017 + ], + "molecules_to_micromolar_conv": 6.2e-05, + "sens": 1.374064, + "sigma_mult": [ + 0.981658, + 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zHLK6k5%^D4J__b5gB6j2dWw4>_67CC@?xoa;15wQhq3cr-l5IR!_{Im-_D&tEXyqz<)0ho{Sl!c_BFwzOF5c JsowBO{tpEYuPgun diff --git a/Analysis/config/xtalk.550.well.settings.json b/Analysis/config/xtalk.550.well.settings.json new file mode 100644 index 00000000..362454f4 --- /dev/null +++ b/Analysis/config/xtalk.550.well.settings.json @@ -0,0 +1 @@ +{"ChipType":"550","GridType":"HexGridCol","MapSpan":25,"NNX":2,"NNY":2,"XtalkCoef":[0.0021,0.0064,0.0059,0.0057,0.0007,0.002,0.0025,0.012,0.0039,0.0018,0.0089,0.0162,0.0001,0.0154,0.0075,0.0021,0.0167,0.0118,0.0154,0.0012,0.001,0.004,0.0059,0.0018,0.0018],"Xtype":"Simple"} diff --git a/Analysis/config/xtalk.p2.3.1.well.settings.json b/Analysis/config/xtalk.p2.3.1.well.settings.json new file mode 100644 index 00000000..cbdeac46 --- /dev/null +++ b/Analysis/config/xtalk.p2.3.1.well.settings.json @@ -0,0 +1 @@ +{"ChipType":"P2.3.1","GridType":"HexGridCol","MapSpan":25,"NNX":2,"NNY":2,"XtalkCoef":[0.0021,0.0064,0.0059,0.0057,0.0007,0.002,0.0025,0.012,0.0039,0.0018,0.0089,0.0162,0.0001,0.0154,0.0075,0.0021,0.0167,0.0118,0.0154,0.0012,0.001,0.004,0.0059,0.0018,0.0018],"Xtype":"Simple"} diff --git a/Analysis/crop/ChkDat.cpp b/Analysis/crop/ChkDat.cpp index 4d813e6a..d1b5a9e5 100755 --- a/Analysis/crop/ChkDat.cpp +++ b/Analysis/crop/ChkDat.cpp @@ -41,7 +41,7 @@ uint32_t numAcq = 0; int dont_wait = 0; char *DstDir = (char *)"."; char *oneFile = NULL; -uint32_t verbose=1; +uint32_t verbose=0; uint32_t Error = 0; char DirList[200][512]; diff --git a/Analysis/crop/Crop.cpp b/Analysis/crop/Crop.cpp index 7d7d2eae..4ac40fcc 100644 --- a/Analysis/crop/Crop.cpp +++ b/Analysis/crop/Crop.cpp @@ -374,6 +374,9 @@ int main ( int argc, char *argv[] ) else options.applyXtalkCorrection2 = 0.2; break; + case 'N': + options.doGainCorrection = 1; + break; case 'O': options.doGainCorrection_bf = 1; break; diff --git a/Analysis/polyclonal_filter.h b/Analysis/polyclonal_filter.h deleted file mode 100644 index 53f0b694..00000000 --- a/Analysis/polyclonal_filter.h +++ /dev/null @@ -1,21 +0,0 @@ -/* Copyright (C) 2014 Ion Torrent Systems, Inc. All Rights Reserved */ - -#ifndef POLYCLONAL_FILTER_H -#define POLYCLONAL_FILTER_H - -class PolyclonalFilterOpts { - public: - PolyclonalFilterOpts(); - - bool enable; - int mixed_first_flow; - int mixed_last_flow; - int max_iterations; - int mixed_model_option; - double mixed_stringency; - bool verbose; - bool use_last_iter_params; - bool filter_extreme_ppf_only; -}; - -#endif // POLYCLONAL_FILTER_H diff --git a/Analysis/realignment/Realigner.cpp b/Analysis/realignment/Realigner.cpp index b0eb15fd..23d125fd 100644 --- a/Analysis/realignment/Realigner.cpp +++ b/Analysis/realignment/Realigner.cpp @@ -220,11 +220,18 @@ void Realigner::SetSequences(const string& q_seq, const string& t_seq, const str if (debug_ and verbose_) cout << "Hello from SetSequences." << endl; + // If we have an empty aln_path, and alignment_bandwidth_>0 we align along the diagonal + // Empty aln_path and alignment_bandwidth_==0 evaluates the whole DP matrix + if (aln_path.length()==0 and alignment_bandwidth_>0){ + create_dummy_pretty(t_seq.length(), q_seq.length()); + } + else + pretty_aln_ = aln_path; + // We align all sequences in forward direction - pretty_aln_ = aln_path; + // but have to worry about clipping the correct end of the alignment q_seq_ = q_seq; t_seq_ = t_seq; - // but have to worry about clipping the correct end of the alignment if (isForwardStrandRead_ != isForward) { ReverseClipping(); isForwardStrandRead_ = isForward; @@ -246,6 +253,33 @@ void Realigner::SetSequences(const string& q_seq, const string& t_seq, const str cout << "Successfully set sequences." << endl; } +// ------------------------------------------------------------------- +// For tubed alignment to work without a prior alignment input +// Create a pseudo diagonal pretty alignment string for initialization + +void Realigner::create_dummy_pretty(unsigned int target_length, unsigned int query_length) +{ + pretty_aln_.assign(max(target_length, query_length), '|'); + unsigned int plen = pretty_aln_.length(); + char indel = '-'; + int n_indels = target_length - query_length; + + if (n_indels==0) + return; + else if (n_indels<0) { + indel = '+'; + n_indels = -n_indels; + } + int interval = plen / n_indels; + unsigned int pos = (plen - ((n_indels-1)*interval))/2; + int count = 0; + + while (count& CigarData, vector& MD_data, unsigned int& start_pos_update) { - string dummy_string; - // Compute boundaries for tubed alignment around previously found alignment if (!ComputeTubedAlignmentBoundaries()) return false; diff --git a/Analysis/realignment/Realigner.h b/Analysis/realignment/Realigner.h index 49526f25..ced16a23 100644 --- a/Analysis/realignment/Realigner.h +++ b/Analysis/realignment/Realigner.h @@ -221,6 +221,9 @@ class Realigner { //! @ brief Spells out the constants representing alignment types string PrintAlignType(int align_type); + //! @ brief - Create a dummy pretty alignment string if none is available + void create_dummy_pretty(unsigned int target_length, unsigned int query_length); + bool start_anywhere_in_ref_; //!< Allow aligned read to start at any point in the reference bool stop_anywhere_in_ref_; //!< Allow aligned read to end at any point in the reference diff --git a/Analysis/version b/Analysis/version index fa476b39..5b986bf6 100644 --- a/Analysis/version +++ b/Analysis/version @@ -1,4 +1,4 @@ # Version for target of current development cycle MAJOR=5 -MINOR=2 -RELEASE=26 +MINOR=4 +RELEASE=11 diff --git a/Analysis/xtalk_sim/DiffEqModel.h b/Analysis/xtalk_sim/DiffEqModel.h index 9da2315a..1a307a3e 100755 --- a/Analysis/xtalk_sim/DiffEqModel.h +++ b/Analysis/xtalk_sim/DiffEqModel.h @@ -18,7 +18,7 @@ typedef enum { #include #include "xtalk_sim.h" -#include "WorkerInfoQueue.h" +#include "../Util/WorkerInfoQueue.h" #include "SignalAverager.h" #include "WellVoxelTracker.h" #include "DelsqCUDA.h" diff --git a/Analysis/xtalk_sim/WorkerInfoQueue.cpp b/Analysis/xtalk_sim/WorkerInfoQueue.cpp deleted file mode 100644 index b1af6d25..00000000 --- a/Analysis/xtalk_sim/WorkerInfoQueue.cpp +++ /dev/null @@ -1,279 +0,0 @@ -/* Copyright (C) 2010 Ion Torrent Systems, Inc. All Rights Reserved */ - -#include "WorkerInfoQueue.h" - -// create a queue w/ that can hold the specified number of items -WorkerInfoQueue::WorkerInfoQueue(int _depth) -{ - depth = _depth; - rdndx = 0; - wrndx = 0; - num = 0; - not_done_cnt = 0; - qlist = new WorkerInfoQueueItem[depth]; - - pthread_mutex_init(&lock, NULL); - pthread_cond_init(&rdcond,NULL); - pthread_cond_init(&wrcond,NULL); - pthread_cond_init(&donecond,NULL); -} - -// put a new item on the queue. this will block if the queue is full -void WorkerInfoQueue::PutItem(WorkerInfoQueueItem &new_item) -{ - // obtain the lock - pthread_mutex_lock(&lock); - - // wait for someone to signal new free space - // fprintf(stdout, "WorkerInfoQueue::PutItem: %d\n", num); - while (num >= depth) - pthread_cond_wait(&wrcond,&lock); - - qlist[wrndx] = new_item; - if (++wrndx >= depth) wrndx = 0; - num++; - not_done_cnt++; - - // signal readers to check for the new item - pthread_cond_signal(&rdcond); - - // give up the lock - pthread_mutex_unlock(&lock); -} - -// remove an item from the queue. this will block if the queue is empty -WorkerInfoQueueItem WorkerInfoQueue::GetItem(void) -{ - WorkerInfoQueueItem item; - - // obtain the lock - pthread_mutex_lock(&lock); - - // wait for someone to signal a new item - // fprintf(stdout, "WorkerInfoQueue::GetItem: %d\n", num); - while (num == 0) - pthread_cond_wait(&rdcond,&lock); - - item = qlist[rdndx]; - if (++rdndx >= depth) rdndx = 0; - num--; - - // signal writers that more free space is available - pthread_cond_signal(&wrcond); - - // give up the lock - pthread_mutex_unlock(&lock); - - return(item); -} - -// try to remove an item from the queue. this will return item with empty data if the queue is empty -WorkerInfoQueueItem WorkerInfoQueue::TryGetItem(void) -{ - WorkerInfoQueueItem item; - - // obtain the lock - pthread_mutex_lock(&lock); - - // wait for someone to signal a new item - if (num == 0) { - pthread_mutex_unlock(&lock); - item.private_data = NULL; - return item; - } - - item = qlist[rdndx]; - if (++rdndx >= depth) rdndx = 0; - num--; - - // signal writers that more free space is available - pthread_cond_signal(&wrcond); - - // give up the lock - pthread_mutex_unlock(&lock); - - return(item); -} - - -// NOTE: just because the q is empty...doesn't mean the workers are done with the -// last item they pulled off. Worker's decrement the 'not done' count whenever they -// finish a work item. This waits till all the work items have been completed -void WorkerInfoQueue::WaitTillDone(void) -{ - // obtain the lock - pthread_mutex_lock(&lock); - - // wait for someone to signal new free space - while (not_done_cnt > 0) - pthread_cond_wait(&donecond,&lock); - - // give up the lock - pthread_mutex_unlock(&lock); -} - -// Allows workers to indicate that they have completed a task -void WorkerInfoQueue::DecrementDone(void) -{ - // obtain the lock - pthread_mutex_lock(&lock); - - // decrement done count - not_done_cnt--; - - // if everything is done, signal the condition change - if (not_done_cnt == 0) - pthread_cond_signal(&donecond); - - // give up the lock - pthread_mutex_unlock(&lock); -} - -WorkerInfoQueue::~WorkerInfoQueue() -{ - pthread_cond_destroy(&donecond); - pthread_cond_destroy(&wrcond); - pthread_cond_destroy(&rdcond); - pthread_mutex_destroy(&lock); - delete [] qlist; -} - - -// create a queue w/ that can hold the specified number of items -DynamicWorkQueueGpuCpu::DynamicWorkQueueGpuCpu(int _depth) -{ - start = false; - depth = _depth; - gpuRdIdx = 0; - cpuRdIdx = depth - 1; - wrIdx = 0; - not_done_cnt = 0; - qlist = new WorkerInfoQueueItem[depth]; - - pthread_mutex_init(&lock, NULL); - pthread_cond_init(&startcond,NULL); - pthread_cond_init(&donecond,NULL); -} - - -WorkerInfoQueueItem DynamicWorkQueueGpuCpu::GetGpuItem() { - - WorkerInfoQueueItem item; - - pthread_mutex_lock(&lock); - //printf("Acquiring lock gpu\n"); - - - while (!start) - pthread_cond_wait(&startcond, &lock); - - if (gpuRdIdx == cpuRdIdx) - start = false; - - //printf("Getting Gpu Item, GpuIdx: %d CpuIdx: %d, start: %d\n", gpuRdIdx, cpuRdIdx, start); - item = qlist[gpuRdIdx++]; - - //printf("Releasing lock gpu\n"); - pthread_mutex_unlock(&lock); - - return item; -} - -WorkerInfoQueueItem DynamicWorkQueueGpuCpu::GetCpuItem() { - - WorkerInfoQueueItem item; - - pthread_mutex_lock(&lock); - - //printf("Acquiring lock cpu\n"); - - - while (!start) - pthread_cond_wait(&startcond, &lock); - - if (cpuRdIdx == gpuRdIdx) - start = false; - - //printf("Getting Cpu Item, GpuIdx: %d CpuIdx: %d, start: %d\n", gpuRdIdx, cpuRdIdx, start); - item = qlist[cpuRdIdx--]; - - //printf("Releasing lock cpu\n"); - pthread_mutex_unlock(&lock); - - return item; -} - -void DynamicWorkQueueGpuCpu::PutItem(WorkerInfoQueueItem &new_item) { - - - qlist[wrIdx++] = new_item; - not_done_cnt++; - - //printf("Putting Item\n"); - if (wrIdx == depth) { - pthread_mutex_lock(&lock); - //printf("Signalling to start\n"); - start = true; - pthread_cond_broadcast(&startcond); - pthread_mutex_unlock(&lock); - } - -} - - -// NOTE: just because the q is empty...doesn't mean the workers are done with the -// last item they pulled off. Worker's decrement the 'not done' count whenever they -// finish a work item. This waits till all the work items have been completed -void DynamicWorkQueueGpuCpu::WaitTillDone(void) -{ - // obtain the lock - pthread_mutex_lock(&lock); - - // wait for someone to signal new free space - while (not_done_cnt > 0) - pthread_cond_wait(&donecond,&lock); - - // give up the lock - pthread_mutex_unlock(&lock); -} - -// Allows workers to indicate that they have completed a task -void DynamicWorkQueueGpuCpu::DecrementDone(void) -{ - // obtain the lock - pthread_mutex_lock(&lock); - - // decrement done count - not_done_cnt--; - - // if everything is done, signal the condition change - if (not_done_cnt == 0) { - start = false; - pthread_cond_signal(&donecond); - } - - // give up the lock - pthread_mutex_unlock(&lock); -} - -void DynamicWorkQueueGpuCpu::ResetIndices() { - pthread_mutex_lock(&lock); - wrIdx = 0; - gpuRdIdx = 0; - cpuRdIdx = depth - 1; - pthread_mutex_unlock(&lock); -} - -int DynamicWorkQueueGpuCpu::getGpuReadIndex() { - return gpuRdIdx; -} - -DynamicWorkQueueGpuCpu::~DynamicWorkQueueGpuCpu() -{ - pthread_cond_destroy(&donecond); - pthread_cond_destroy(&startcond); - pthread_mutex_destroy(&lock); - delete [] qlist; -} - - diff --git a/Analysis/xtalk_sim/WorkerInfoQueue.h b/Analysis/xtalk_sim/WorkerInfoQueue.h deleted file mode 100644 index 10b26ba6..00000000 --- a/Analysis/xtalk_sim/WorkerInfoQueue.h +++ /dev/null @@ -1,339 +0,0 @@ -/* Copyright (C) 2010 Ion Torrent Systems, Inc. All Rights Reserved */ -#ifndef WORKERINFOQUEUE_H -#define WORKERINFOQUEUE_H - -#include -#include -#include -#include -#include -#include -#include -#include -#include -#include -#include -#include -#include -#include -#include -#include -#include -#include -#include -#include - - -struct WorkerInfoQueueItem -{ - bool finished; - void *private_data; - WorkerInfoQueueItem() - { - finished = false; - private_data = NULL; - } -}; - -/// helper class to distribute work items to a set of threads for processing -/// add class access is thread safe -class WorkerInfoQueue -{ - public: - /** Create a queue w/ that can hold the specified number of items */ - WorkerInfoQueue(int _depth); - - /** Put a new item on the queue. Blocks if the queue is full */ - void PutItem(WorkerInfoQueueItem &new_item); - - /** remove an item from the queue. this will block if the queue is empty */ - WorkerInfoQueueItem GetItem(void); - - /** try to remove an item from the queue. this will return item with empty data if the queue is empty */ - WorkerInfoQueueItem TryGetItem(void); - - // NOTE: just because the q is empty...doesn't mean the workers are done with the - // last item they pulled off. Worker's decrement the 'not done' count whenever they - // finish a work item. - /** Wait till all the work items have been completed */ - void WaitTillDone(void); - - /* Call when a worker has completed a task */ - void DecrementDone(void); - - int GetNumNotDone(void) { return not_done_cnt; } - - ~WorkerInfoQueue(); - -private: - int rdndx; - int wrndx; - int num; - int not_done_cnt; - int depth; - WorkerInfoQueueItem *qlist; - - // synchronization objects - pthread_mutex_t lock; - pthread_cond_t wrcond; - pthread_cond_t rdcond; - pthread_cond_t donecond; -}; - -class DynamicWorkQueueGpuCpu { - -public: - - // create a queue w/ that can hold the specified number of items - DynamicWorkQueueGpuCpu(int _depth); - - WorkerInfoQueueItem GetGpuItem(); - WorkerInfoQueueItem GetCpuItem(); - - void PutItem(WorkerInfoQueueItem &new_item); - - // NOTE: just because the q is empty...doesn't mean the workers are done with the - // last item they pulled off. Worker's decrement the 'not done' count whenever they - // finish a work item. This waits till all the work items have been completed - void WaitTillDone(void); - - // Allows workers to indicate that they have completed a task - void DecrementDone(void); - - void ResetIndices(); - int getGpuReadIndex(); - - ~DynamicWorkQueueGpuCpu(); - -private: - int gpuRdIdx; - int cpuRdIdx; - int wrIdx; - int depth; - int not_done_cnt; - bool start; - WorkerInfoQueueItem *qlist; - - // synchronization objects - pthread_mutex_t lock; - pthread_cond_t donecond; - pthread_cond_t startcond; -}; - - -/* -#ifndef __FRAME_OUTPUT_QUEUE_H -#define __FRAME_OUTPUT_QUEUE_H - -#include - -struct frame_info -{ - int frameNumber; - UINT8 *ptr; - struct timeval timestamp; - UINT32 frame_duration; -}; - -struct FrameOutputQueue -{ - struct frame_info *frames; - int rd_ndx; - int wr_ndx; - int num; - int nFrames; - int acqFinished; - - pthread_mutex_t lock; - pthread_cond_t wrcond; - pthread_cond_t rdcond; -}; - -// Creates a new output queue large enough to hold numFrames -struct FrameOutputQueue *foq_Create(int numFrames); - -// Destroys a FrameOutputQueue and free's the resources used by the control structures -void foq_Destroy(struct FrameOutputQueue *q); - -// Puts a new frame into the queue. Blocks if there isn't space for the new frame -void foq_PutFrame(struct FrameOutputQueue *q,int frameNum,struct timeval timestamp,UINT32 frame_duration,UINT8 *ptr); - -// Gets the next frame from the queue. Blocks if there aren't any new frames available -int foq_GetFrame(struct FrameOutputQueue *q,struct frame_info *pframe); - -// indicate to readers that the acquisition is complete and no more frames should be -// expected -void foq_FinishAcquisition(struct FrameOutputQueue *q); - -// Empty's the queue -void foq_Reset(struct FrameOutputQueue *q); - -// Queries number of frames in the queue -int foq_GetNum(struct FrameOutputQueue *q); - -#endif - - - #include "datacollect_global.h" -#include "FrameOutputQueue.h" - -#include "dbgmem.h" - -// Creates a new output queue large enough to hold numFrames -struct FrameOutputQueue *foq_Create(int numFrames) -{ - struct frame_info *frames; - struct FrameOutputQueue *q; - - q = (struct FrameOutputQueue *)malloc(sizeof(struct FrameOutputQueue)); - - if (q == NULL) - goto err0; - - frames = (struct frame_info *)malloc(sizeof(struct frame_info)*numFrames); - - if (frames == NULL) - goto err1; - - q->frames = frames; - - if (pthread_mutex_init(&q->lock, NULL) != 0) - goto err2; - - if (pthread_cond_init(&q->rdcond,NULL) != 0) - goto err3; - - if (pthread_cond_init(&q->wrcond,NULL) != 0) - goto err4; - - q->nFrames = numFrames; - foq_Reset(q); - - return(q); - -err4: - pthread_cond_destroy(&q->rdcond); -err3: - pthread_mutex_destroy(&q->lock); -err2: - free(frames); -err1: - free(q); -err0: - return NULL; -} - -// Destroys a FrameOutputQueue and free's the resources used by the control structures -void foq_Destroy(struct FrameOutputQueue *q) -{ - // technically...I should check here to see if anyone is blocked on these - // things before destroying them...will fix this at some point - pthread_cond_destroy(&q->wrcond); - pthread_cond_destroy(&q->rdcond); - pthread_mutex_destroy(&q->lock); - free(q->frames); - free(q); -} - -// Puts a new frame into the queue. Blocks if there isn't space for the new frame -void foq_PutFrame(struct FrameOutputQueue *q,int frameNum,struct timeval timestamp,UINT32 frame_duration,UINT8 *ptr) -{ - pthread_mutex_lock(&q->lock); - - while (q->num >= q->nFrames) - { - // wait for someone to signal new free space - pthread_cond_wait(&q->wrcond,&q->lock); - } - - q->frames[q->wr_ndx].frameNumber = frameNum; - q->frames[q->wr_ndx].timestamp = timestamp; - q->frames[q->wr_ndx].frame_duration = frame_duration; - q->frames[q->wr_ndx].ptr = ptr; - - if (++(q->wr_ndx) >= q->nFrames) q->wr_ndx = 0; - q->num++; - - // signal readers to check for the new frame - pthread_cond_signal(&q->rdcond); - - pthread_mutex_unlock(&q->lock); -} - -// Gets the next frame from the queue. Blocks if there aren't any new frames available -int foq_GetFrame(struct FrameOutputQueue *q,struct frame_info *pframe) -{ - int nrcv = 0; - - pthread_mutex_lock(&q->lock); - - while ((q->num <= 0) && (q->acqFinished == 0)) - { - // wait for someone to signal new frame - pthread_cond_wait(&q->rdcond,&q->lock); - } - - // if there was a new frame available, get the info - if (q->num > 0) - { - pframe->frameNumber = q->frames[q->rd_ndx].frameNumber; - pframe->timestamp = q->frames[q->rd_ndx].timestamp; - pframe->ptr = q->frames[q->rd_ndx].ptr; - pframe->frame_duration = q->frames[q->rd_ndx].frame_duration; - - if (++(q->rd_ndx) >= q->nFrames) q->rd_ndx = 0; - q->num--; - - // signal new free space to writers - pthread_cond_signal(&q->wrcond); - - nrcv = 1; - } - - pthread_mutex_unlock(&q->lock); - - // if we return 0, it means there aren't any more frames available - // and the acqusition has finished...so don't expect any more - return nrcv; -} - -// indicate to readers that the acquisition is complete and no more frames should be -// expected -void foq_FinishAcquisition(struct FrameOutputQueue *q) -{ - pthread_mutex_lock(&q->lock); - - q->acqFinished = 1; - pthread_cond_signal(&q->rdcond); - - pthread_mutex_unlock(&q->lock); -} - -// Empty's the queue -void foq_Reset(struct FrameOutputQueue *q) -{ - pthread_mutex_lock(&q->lock); - - q->acqFinished = q->rd_ndx = q->wr_ndx = q->num = 0; - - pthread_mutex_unlock(&q->lock); -} - -// Queries number of frames in the queue -int foq_GetNum(struct FrameOutputQueue *q) -{ - int ret; - - pthread_mutex_lock(&q->lock); - - ret = q->num; - - pthread_mutex_unlock(&q->lock); - - return(ret); -} - - -*/ - -#endif // WORKERINFOQUEUE_H diff --git a/RSM/version b/RSM/version index 183b81a6..a37e111d 100644 --- a/RSM/version +++ b/RSM/version @@ -1,4 +1,4 @@ # Version for target of current development cycle MAJOR=5 -MINOR=2 -RELEASE=5 +MINOR=4 +RELEASE=0 diff --git a/TSVersion b/TSVersion index 7820c525..45b60990 100644 --- a/TSVersion +++ b/TSVersion @@ -1,4 +1,4 @@ # Version for target of current development cycle TS_MAJOR=5 -TS_MINOR=2 -TS_RELEASE=2 +TS_MINOR=4 +TS_RELEASE=0 diff --git a/buildTools/BUILD.txt b/buildTools/BUILD.txt index 4a1de59b..2054e556 100644 --- a/buildTools/BUILD.txt +++ b/buildTools/BUILD.txt @@ -51,7 +51,6 @@ QUICK START on Ubuntu 11.10 (=Oneiric) (EXPERIMENTAL) libarmadillo-dev \ libboost-serialization-dev \ libncurses-dev \ - libbz2-dev libbz2-dev \ libcurl4-openssl-dev \ libncurses-dev \ diff --git a/buildTools/LICENSE.txt.in b/buildTools/LICENSE.txt.in index 2f53b08f..34102a71 100644 --- a/buildTools/LICENSE.txt.in +++ b/buildTools/LICENSE.txt.in @@ -1,489 +1,649 @@ @CMAKE_PROJECT_NAME@ -- @PROJECT_DESCRIPTION@ +Torrent Suite Software Version 5.4 ===================================================================== -1. Torrent Suite Software License -===================================================================== - -Torrent Suite Software: This software provides for the analysis and storage of data received from the Ion Personal Genome Machine(R) PGM(tm) Sequencer or the Ion Proton(R) Sequencer. ---------------------------------------------------------------------- - -Copyright (C) 2011 Life Technologies Corporation +Torrent Suite(tm) Software v5.4: This software provides for the analysis and storage of data received from the Ion Personal Genome Machine(tm) Sequencer, the Ion Proton(tm) Sequencer, the Ion S5(tm) Sequencer or the Ion S5(tm) XL Sequencer. +April 19, 2017 +Copyright (C) 2017 Life Technologies Corporation All Rights Reserved. This program is free software; you can redistribute it and/or modify it -under the terms of the GNU General Public License as published by the -Free Software Foundation; either version 2 of the License, or (at your -option) any later version. +under the terms of the GNU General Public License version 2 as published by the +Free Software Foundation. This program is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without even the implied warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU -General Public License for more details. +General Public License version 2 for more details. -You should have received a copy of the GNU General Public License along -with this program; if not, write to the Free Software Foundation, Inc., -59 Temple Place - Suite 330, Boston, MA 02111-1307, USA. You can also find the -GPL on the GNU web site: . +You should have received a copy of the GNU General Public License version 2 along +with this program (see Section 2 below); if not, write to the Free Software Foundation, Inc., +59 Temple Place - Suite 330, Boston, MA 02111-1307, USA. You can also find the +GPL version 2 on the GNU web site: https://www.gnu.org/licenses/gpl-2.0.html + +1. Third Party Software Notices and Licenses ===================================================================== -2. Third Party Software Notices and Licenses. -===================================================================== + +This software uses third party software components from several sources. Portions of these software components are copyrighted and licensed by their respective owners as indicated below. Various licenses require distribution of source code or if a link is used to point the end-user to a source-code repository, and the source code is not available at such site, the distributor must, for a time determined by license, offer to provide source code. In such cases, please contact your Life Technologies representative. As well, various licenses require that the end-user receive a copy of the license or certain notices. Such licenses and notices may be found below. In order to use this software, the end-user must abide by the terms and conditions of these third party licenses. + +Armadillo +------------------------------------------------------------- + +Mozilla Public License Version 2.0 +------------------------------------------------------------- + +1. Definitions +------------------------------------------------------------- + +1.1. "Contributor" + means each individual or legal entity that creates, contributes to + the creation of, or owns Covered Software. +1.2. "Contributor Version" + means the combination of the Contributions of others (if any) used + by a Contributor and that particular Contributor's Contribution. + +1.3. "Contribution" + means Covered Software of a particular Contributor. + +1.4. "Covered Software" + means Source Code Form to which the initial Contributor has attached + the notice in Exhibit A, the Executable Form of such Source Code + Form, and Modifications of such Source Code Form, in each case + including portions thereof. + +1.5. "Incompatible With Secondary Licenses" + means + + (a) that the initial Contributor has attached the notice described + in Exhibit B to the Covered Software; or + + (b) that the Covered Software was made available under the terms of + version 1.1 or earlier of the License, but not also under the + terms of a Secondary License. + +1.6. "Executable Form" + means any form of the work other than Source Code Form. + +1.7. "Larger Work" + means a work that combines Covered Software with other material, in + a separate file or files, that is not Covered Software. + +1.8. "License" + means this document. + +1.9. "Licensable" + means having the right to grant, to the maximum extent possible, + whether at the time of the initial grant or subsequently, any and + all of the rights conveyed by this License. + +1.10. "Modifications" + means any of the following: + + (a) any file in Source Code Form that results from an addition to, + deletion from, or modification of the contents of Covered + Software; or -The following third party software components are included in the Torrent Suite -Software. These software components are copyrighted and licensed by their respective -owners. Various components require distribution of source code or if a URL is used to -point the end-user to a source-code repository, and the source code is not available at -such site, the distributor must, for a time determined by the license, offer to provide the -source code. In such cases, please contact your Ion Torrent representative. As well, -various licenses require that the end-user receive a copy of the license and such licenses -may be found below. In order to use this Torrent Suite Software, the end-user must -abide by the terms and conditions of these third-party licenses. + (b) any new file in Source Code Form that contains any Covered + Software. -lapack -URL: http://www.netlib.org/lapack/ -License: BSD +1.11. "Patent Claims" of a Contributor + means any patent claim(s), including without limitation, method, + process, and apparatus claims, in any patent Licensable by such + Contributor that would be infringed, but for the grant of the + License, by the making, using, selling, offering for sale, having + made, import, or transfer of either its Contributions or its + Contributor Version. -k-means -URL: http://www.cs.umd.edu/~mount/Projects/KMeans/ -License: GPL +1.12. "Secondary License" + means either the GNU General Public License, Version 2.0, the GNU + Lesser General Public License, Version 2.1, the GNU Affero General + Public License, Version 3.0, or any later versions of those + licenses. -boost -URL: http://www.boost.org/ -License: BSD +1.13. "Source Code Form" + means the form of the work preferred for making modifications. -atlas -URL: http://math-atlas.sourceforge.net -License: BSD +1.14. "You" (or "Your") + means an individual or a legal entity exercising rights under this + License. For legal entities, "You" includes any entity that + controls, is controlled by, or is under common control with You. For + purposes of this definition, "control" means (a) the power, direct + or indirect, to cause the direction or management of such entity, + whether by contract or otherwise, or (b) ownership of more than + fifty percent (50%) of the outstanding shares or beneficial + ownership of such entity. -libgfortran -License: GPL +2. License Grants and Conditions +------------------------------------------------------------- -LAPACK ---------------------------------------------------------------------- -Copyright (c) 1992-2008 The University of Tennessee. All rights -reserved. +2.1. Grants -$COPYRIGHT$ +Each Contributor hereby grants You a world-wide, royalty-free, +non-exclusive license: -Additional copyrights may follow +(a) under intellectual property rights (other than patent or trademark) + Licensable by such Contributor to use, reproduce, make available, + modify, display, perform, distribute, and otherwise exploit its + Contributions, either on an unmodified basis, with Modifications, or + as part of a Larger Work; and -$HEADER$ +(b) under Patent Claims of such Contributor to make, use, sell, offer + for sale, have made, import, and otherwise transfer either its + Contributions or its Contributor Version. + +2.2. Effective Date + +The licenses granted in Section 2.1 with respect to any Contribution +become effective for each Contribution on the date the Contributor first +distributes such Contribution. + +2.3. Limitations on Grant Scope + +The licenses granted in this Section 2 are the only rights granted under +this License. No additional rights or licenses will be implied from the +distribution or licensing of Covered Software under this License. +Notwithstanding Section 2.1(b) above, no patent license is granted by a +Contributor: + +(a) for any code that a Contributor has removed from Covered Software; + or + +(b) for infringements caused by: (i) Your and any other third party's + modifications of Covered Software, or (ii) the combination of its + Contributions with other software (except as part of its Contributor + Version); or + +(c) under Patent Claims infringed by Covered Software in the absence of + its Contributions. + +This License does not grant any rights in the trademarks, service marks, +or logos of any Contributor (except as may be necessary to comply with +the notice requirements in Section 3.4). + +2.4. Subsequent Licenses + +No Contributor makes additional grants as a result of Your choice to +distribute the Covered Software under a subsequent version of this +License (see Section 10.2) or under the terms of a Secondary License (if +permitted under the terms of Section 3.3). + +2.5. Representation + +Each Contributor represents that the Contributor believes its +Contributions are its original creation(s) or it has sufficient rights +to grant the rights to its Contributions conveyed by this License. + +2.6. Fair Use + +This License is not intended to limit any rights You have under +applicable copyright doctrines of fair use, fair dealing, or other +equivalents. + +2.7. Conditions + +Sections 3.1, 3.2, 3.3, and 3.4 are conditions of the licenses granted +in Section 2.1. + +3. Responsibilities +------------------------------------------------------------- + +3.1. Distribution of Source Form + +All distribution of Covered Software in Source Code Form, including any +Modifications that You create or to which You contribute, must be under +the terms of this License. You must inform recipients that the Source +Code Form of the Covered Software is governed by the terms of this +License, and how they can obtain a copy of this License. You may not +attempt to alter or restrict the recipients' rights in the Source Code +Form. + +3.2. Distribution of Executable Form + +If You distribute Covered Software in Executable Form then: + +(a) such Covered Software must also be made available in Source Code + Form, as described in Section 3.1, and You must inform recipients of + the Executable Form how they can obtain a copy of such Source Code + Form by reasonable means in a timely manner, at a charge no more + than the cost of distribution to the recipient; and + +(b) You may distribute such Executable Form under the terms of this + License, or sublicense it under different terms, provided that the + license for the Executable Form does not attempt to limit or alter + the recipients' rights in the Source Code Form under this License. + +3.3. Distribution of a Larger Work + +You may create and distribute a Larger Work under terms of Your choice, +provided that You also comply with the requirements of this License for +the Covered Software. If the Larger Work is a combination of Covered +Software with a work governed by one or more Secondary Licenses, and the +Covered Software is not Incompatible With Secondary Licenses, this +License permits You to additionally distribute such Covered Software +under the terms of such Secondary License(s), so that the recipient of +the Larger Work may, at their option, further distribute the Covered +Software under the terms of either this License or such Secondary +License(s). + +3.4. Notices + +You may not remove or alter the substance of any license notices +(including copyright notices, patent notices, disclaimers of warranty, +or limitations of liability) contained within the Source Code Form of +the Covered Software, except that You may alter any license notices to +the extent required to remedy known factual inaccuracies. + +3.5. Application of Additional Terms + +You may choose to offer, and to charge a fee for, warranty, support, +indemnity or liability obligations to one or more recipients of Covered +Software. However, You may do so only on Your own behalf, and not on +behalf of any Contributor. You must make it absolutely clear that any +such warranty, support, indemnity, or liability obligation is offered by +You alone, and You hereby agree to indemnify every Contributor for any +liability incurred by such Contributor as a result of warranty, support, +indemnity or liability terms You offer. You may include additional +disclaimers of warranty and limitations of liability specific to any +jurisdiction. + +4. Inability to Comply Due to Statute or Regulation +--------------------------------------------------- + +If it is impossible for You to comply with any of the terms of this +License with respect to some or all of the Covered Software due to +statute, judicial order, or regulation then You must: (a) comply with +the terms of this License to the maximum extent possible; and (b) +describe the limitations and the code they affect. Such description must +be placed in a text file included with all distributions of the Covered +Software under this License. Except to the extent prohibited by statute +or regulation, such description must be sufficiently detailed for a +recipient of ordinary skill to be able to understand it. + +5. Termination +------------------------------------------------------------- + +5.1. The rights granted under this License will terminate automatically +if You fail to comply with any of its terms. However, if You become +compliant, then the rights granted under this License from a particular +Contributor are reinstated (a) provisionally, unless and until such +Contributor explicitly and finally terminates Your grants, and (b) on an +ongoing basis, if such Contributor fails to notify You of the +non-compliance by some reasonable means prior to 60 days after You have +come back into compliance. Moreover, Your grants from a particular +Contributor are reinstated on an ongoing basis if such Contributor +notifies You of the non-compliance by some reasonable means, this is the +first time You have received notice of non-compliance with this License +from such Contributor, and You become compliant prior to 30 days after +Your receipt of the notice. + +5.2. If You initiate litigation against any entity by asserting a patent +infringement claim (excluding declaratory judgment actions, +counter-claims, and cross-claims) alleging that a Contributor Version +directly or indirectly infringes any patent, then the rights granted to +You by any and all Contributors for the Covered Software under Section +2.1 of this License shall terminate. + +5.3. In the event of termination under Sections 5.1 or 5.2 above, all +end user license agreements (excluding distributors and resellers) which +have been validly granted by You or Your distributors under this License +prior to termination shall survive termination. + + + +6. Disclaimer of Warranty +------------------------------------------------------------- + +Covered Software is provided under this License on an "as is" basis, without +warranty of any kind, either expressed, implied, or statutory, including, +without limitation, warranties that the Covered Software is free of defects, +merchantable, fit for a particular purpose or non-infringing. The entire risk +as to the quality and performance of the Covered Software is with You. Should +any Covered Software prove defective in any respect, You (not any Contributor) +assume the cost of any necessary servicing, repair, or correction. This +disclaimer of warranty constitutes an essential part of this License. No use of +any Covered Software is authorized under this License except under this +disclaimer. + + +7. Limitation of Liability +------------------------------------------------------------- + +Under no circumstances and under no legal theory, whether tort (including +negligence), contract, or otherwise, shall any Contributor, or anyone who +distributes Covered Software as permitted above, be liable to You for any +direct, indirect, special, incidental, or consequential damages of any +character including, without limitation, damages for lost profits, loss of +goodwill, work stoppage, computer failure or malfunction, or any and all other +commercial damages or losses, even if such party shall have been informed of +the possibility of such damages. This limitation of liability shall not apply +to liability for death or personal injury resulting from such party's +negligence to the extent applicable law prohibits such limitation. Some +jurisdictions do not allow the exclusion or limitation of incidental or +consequential damages, so this exclusion and limitation may not apply to You. + + +8. Litigation +------------------------------------------------------------- + +Any litigation relating to this License may be brought only in the +courts of a jurisdiction where the defendant maintains its principal +place of business and such litigation shall be governed by laws of that +jurisdiction, without reference to its conflict-of-law provisions. +Nothing in this Section shall prevent a party's ability to bring +cross-claims or counter-claims. + +9. Miscellaneous +------------------------------------------------------------- + +This License represents the complete agreement concerning the subject +matter hereof. If any provision of this License is held to be +unenforceable, such provision shall be reformed only to the extent +necessary to make it enforceable. Any law or regulation which provides +that the language of a contract shall be construed against the drafter +shall not be used to construe this License against a Contributor. + +10. Versions of the License +------------------------------------------------------------- + +10.1. New Versions + +Mozilla Foundation is the license steward. Except as provided in Section +10.3, no one other than the license steward has the right to modify or +publish new versions of this License. Each version will be given a +distinguishing version number. + +10.2. Effect of New Versions + +You may distribute the Covered Software under the terms of the version +of the License under which You originally received the Covered Software, +or under the terms of any subsequent version published by the license +steward. + +10.3. Modified Versions + +If you create software not governed by this License, and you want to +create a new license for such software, you may create and use a +modified version of this License if you rename the license and remove +any references to the name of the license steward (except to note that +such modified license differs from this License). + +10.4. Distributing Source Code Form that is Incompatible With Secondary +Licenses + +If You choose to distribute Source Code Form that is Incompatible With +Secondary Licenses under the terms of this version of the License, the +notice described in Exhibit B of this License must be attached. + +Exhibit A - Source Code Form License Notice +------------------------------------------------------------- + +This Source Code Form is subject to the terms of the Mozilla Public +License, v. 2.0. If a copy of the MPL was not distributed with this +file, You can obtain one at http://mozilla.org/MPL/2.0/. + +If it is not possible or desirable to put the notice in a particular +file, then You may include the notice in a location (such as a LICENSE +file in a relevant directory) where a recipient would be likely to look +for such a notice. + +You may add additional accurate notices of copyright ownership. + +Exhibit B - "Incompatible With Secondary Licenses" Notice +------------------------------------------------------------- + +This Source Code Form is "Incompatible With Secondary Licenses", as +defined by the Mozilla Public License, v. 2.0. + + +HTS Lib and Samtools +------------------------------------------------------------- + +The MIT/Expat License +Copyright (C) 2008-2014 Genome Research Ltd. + +Permission is hereby granted, free of charge, to any person obtaining a copy +of this software and associated documentation files (the "Software"), to deal +in the Software without restriction, including without limitation the rights +to use, copy, modify, merge, publish, distribute, sublicense, and/or sell +copies of the Software, and to permit persons to whom the Software is +furnished to do so, subject to the following conditions: +The above copyright notice and this permission notice shall be included in +all copies or substantial portions of the Software. +THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR +IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, +FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL +THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER +LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING +FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER +DEALINGS IN THE SOFTWARE. + + +VCF Lib +------------------------------------------------------------- + +The MIT License (see text above) +Copyright (c) 2012 Erik Garrison + +Blas and Lapack +------------------------------------------------------------- +Copyright (c) 1992-2013 The University of Tennessee and The University of Tennessee Research Foundation. All rights reserved. + +Copyright (c) 2000-2013 The University of California Berkeley. All rights reserved. + +Copyright (c) 2006-2013 The University of Colorado Denver. All rights reserved. Redistribution and use in source and binary forms, with or without modification, are permitted provided that the following conditions are met: -- Redistributions of source code must retain the above copyright - notice, this list of conditions and the following disclaimer. - -- Redistributions in binary form must reproduce the above copyright - notice, this list of conditions and the following disclaimer listed - in this license in the documentation and/or other materials - provided with the distribution. - -- Neither the name of the copyright holders nor the names of its - contributors may be used to endorse or promote products derived from - this software without specific prior written permission. - + - Redistributions of source code must retain the above copyright + notice, this list of conditions and the following disclaimer. + - Redistributions in binary form must reproduce the above copyright + notice, this list of conditions and the following disclaimer listed + in this license in the documentation and/or other materials + provided with the distribution. + - Neither the name of the copyright holders nor the names of its + contributors may be used to endorse or promote products derived from + this software without specific prior written permission. + +The copyright holders provide no reassurances that the source code +provided does not infringe any patent, copyright, or any other +intellectual property rights of third parties. The copyright holders +disclaim any liability to any recipient for claims brought against +recipient by any third party for infringement of that parties +intellectual property rights. + THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS -"AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT +"AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR -A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE COPYRIGHT +A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE COPYRIGHT OWNER OR CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY -THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT +THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE -OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH -DAMAGE. +OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE. +Django +------------------------------------------------------------- +Copyright (c) Django Software Foundation and individual contributors. All rights reserved. -K-MEANS ---------------------------------------------------------------------- -KMlocal: A testbed for k-means clustering algorithms based on local -search -Version: 1.7 -Date: 08/10/2005 +Redistribution and use in source and binary forms, with or without modification, +are permitted provided that the following conditions are met: -Copyright (C) 2004-2005 David M. Mount and University of Maryland -All Rights Reserved. +1. Redistributions of source code must retain the above copyright notice, + this list of conditions and the following disclaimer. +2. Redistributions in binary form must reproduce the above copyright + notice, this list of conditions and the following disclaimer in the + documentation and/or other materials provided with the distribution. +3. Neither the name of Django nor the names of its contributors may be used + to endorse or promote products derived from this software without + specific prior written permission. + +THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS "AS IS" AND +ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED +WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE +DISCLAIMED. IN NO EVENT SHALL THE COPYRIGHT OWNER OR CONTRIBUTORS BE LIABLE FOR +ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES +(INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; +LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON +ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT +(INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THIS +SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE. + +Cuda Toolkit +------------------------------------------------------------- +END USER LICENSE AGREEMENT (EULA) + +Important Notice READ CAREFULLY: This Software License Agreement ("Agreement") for NVIDIA CUDA Toolkit, including computer software and associated documentation ("Software"), is the Agreement which governs use of the SOFTWARE of NVIDIA Corporation and its subsidiaries ("NVIDIA") downloadable herefrom. By downloading, installing, copying, or otherwise using the SOFTWARE, You (as defined below) agree to be bound by the terms of this Agreement. If You do not agree to the terms of this Agreement, do not download the SOFTWARE. Recitals Use of NVIDIA's SOFTWARE requires three elements: the SOFTWARE, an NVIDIA GPU or application processor ("NVIDIA Hardware"), and a computer system. The SOFTWARE is protected by copyright laws and international copyright treaties, as well as other intellectual property laws and treaties. The SOFTWARE is not sold, and instead is only licensed for Your use, strictly in accordance with this Agreement. The NVIDIA Hardware is protected by various patents, and is sold, but this Agreement does not cover the sale or use of such hardware, since it may not necessarily be sold as a package with the SOFTWARE. This Agreement sets forth the terms and conditions of the SOFTWARE only. + +1.1. Definitions + + 1.1.1. Licensee "You", or "Your" shall mean the entity or individual that downloads and uses the SOFTWARE. + + 1.1.2. Redistributable Software "Redistributable Software" shall mean the redistributable libraries referenced in Attachment A of this Agreement. + + 1.1.3. Software "SOFTWARE" shall mean the deliverables provided pursuant to this Agreement. SOFTWARE may be provided in either source or binary form, at NVIDIA's discretion. + +1.2. Grant of License + + 1.2.1. Rights and Limitations of Grant Provided that Licensee complies with the terms of this Agreement, NVIDIA hereby grants Licensee the following limited, non-exclusive, non-transferable, non-sublicensable (except as expressly permitted otherwise for Redistributable Software in Section 1.2.1.1 and Section 1.2.1.3 of this Agreement) right to use the SOFTWARE -- and, if the SOFTWARE is provided in source form, to compile the SOFTWARE -- with the following limitations: + + 1.2.1.1. Redistribution Rights Licensee may transfer, redistribute, and sublicense certain files of the Redistributable SOFTWARE, as defined in Attachment A of this Agreement, provided, however, that (a) the Redistributable SOFTWARE shall be distributed solely in binary form to Licensee's licensees ("Customers") only as a component of Licensee's own software products (each, a "Licensee Application"); (b) Licensee shall design the Licensee Application such that the Redistributable SOFTWARE files are installed only in a private (non-shared) directory location that is used only by the Licensee Application; (c) Licensee shall obtain each Customer's written or clickwrap agreement to the license terms under a written, legally enforceable agreement that has the effect of protecting the SOFTWARE and the rights of NVIDIA under terms no less restrictive than this Agreement. + + 1.2.1.2. Usage Rights Licensee may install and use multiple copies of the SOFTWARE on a shared computer or concurrently on different computers, and make multiple back-up copies of the SOFTWARE, solely for Licensee's use within Licensee's Enterprise. "Enterprise" shall mean individual use by Licensee or any legal entity (such as a corporation or university) and the subsidiaries it owns by more than 50 percent. + + 1.2.1.3. Further Redistribution Rights Subject to the terms and conditions of the Agreement, Licensee may authorize Customers to further redistribute the Redistributable SOFTWARE that such Customers receive as part of the Licensee Application, solely in binary form, provided, however, that Licensee shall require in their standard software license agreements with Customers that all such redistributions must be made pursuant to a license agreement that has the effect of protecting the SOFTWARE and the rights of NVIDIA whose terms and conditions are at least as restrictive as those in the applicable Licensee software license agreement covering the Licensee Application. For avoidance of doubt, termination of this Agreement shall not affect rights previously granted by Licensee to its Customers under this Agreement to the extent validly granted to Customers under Section 1.2.1.1. + + 1.2.1.4. Linux/FreeBSD Exception Notwithstanding the foregoing terms of Section 1.2.1.2, Section 1.2.1.1 and Section 1.2.1.3, SOFTWARE designed exclusively for use on the Linux or FreeBSD operating systems, or other operating systems derived from the source code to these operating systems, may be copied and redistributed, provided that the binary files thereof are not modified in any way (except for unzipping of compressed files). + + 1.2.1.5. Additional Licensing Obligations Licensee acknowledges and agrees that its use of certain third party components included with the SOFTWARE may be subject to additional licensing terms and conditions as set forth or referenced in Attachment B of this Agreement. + + 1.2.1.6. Limitations No Reverse Engineering If the SOFTWARE is provided in binary form, Licensee may not reverse engineer, decompile, or disassemble the SOFTWARE, nor attempt in any other manner to obtain the source code. No Separation of Components The SOFTWARE is licensed as a single product. Except as authorized in this Agreement, Software component parts of the Software may not be separated for use on more than one computer, nor otherwise used separately from the other parts. No Rental Licensee may not rent or lease the SOFTWARE to someone else. +No Modifications +If the SOFTWARE is provided in source form, Licensee may not modify or create derivative works of the SOFTWARE. + +1.3. Term and Termination This Agreement will continue in effect for two (2) years ("Initial Term") after Your initial download and use of the SOFTWARE, subject to the exclusive right of NVIDIA to terminate as provided herein. The term of this Agreement will automatically renew for successive one (1) year renewal terms after the Initial Term, unless either party provides to the other party at least three (3) months prior written notice of termination before the end of the applicable renewal term. This Agreement will automatically terminate if Licensee fails to comply with any of the terms and conditions hereof. In such event, Licensee must destroy all copies of the SOFTWARE and all of its component parts. Defensive Suspension If Licensee commences or participates in any legal proceeding against NVIDIA, then NVIDIA may, in its sole discretion, suspend or terminate all license grants and any other rights provided under this Agreement during the pendency of such legal proceedings. -This program is free software; you can redistribute it and/or modify it -under the terms of the GNU General Public License as published by the -Free Software Foundation; either version 2 of the License, or (at your -option) any later version. +1.4. Copyright All rights, title, interest and copyrights in and to the SOFTWARE (including but not limited to all images, photographs, animations, video, audio, music, text, and other information incorporated into the SOFTWARE), the accompanying printed materials, and any copies of the SOFTWARE, are owned by NVIDIA, or its suppliers. The SOFTWARE is protected by copyright laws and international treaty provisions. Accordingly, Licensee is required to treat the SOFTWARE like any other copyrighted material, except as otherwise allowed pursuant to this Agreement and that it may make one copy of the SOFTWARE solely for backup or archive purposes. RESTRICTED RIGHTS NOTICE. Software has been developed entirely at private expense and is commercial computer software provided with RESTRICTED RIGHTS. Use, duplication or disclosure by the U.S. Government or a U.S. Government subcontractor is subject to the restrictions set forth in the Agreement under which Software was obtained pursuant to DFARS 227.7202-3(a) or as set forth in subparagraphs (c)(1) and (2) of the Commercial Computer Software - Restricted Rights clause at FAR 52.227-19, as applicable. Contractor/manufacturer is NVIDIA, 2701 San Tomas Expressway, Santa Clara, CA 95050. -This program is distributed in the hope that it will be useful, but -WITHOUT ANY WARRANTY; without even the implied warranty of -MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU -General Public License for more details. - -You should have received a copy of the GNU General Public License along -with this program; if not, write to the Free Software Foundation, Inc., -59 Temple Place - Suite 330, Boston, MA 02111-1307, USA. - -Disclaimer ----------- -The University of Maryland and the authors make no representations -about the suitability or fitness of this software for any purpose. -It is provided "as is" without express or implied warranty. - -Author ------- -David Mount -Dept of Computer Science -University of Maryland, -College Park, MD 20742 USA -mount@cs.umd.edu -http://www.cs.umd.edu/~mount/ - - - -BOOST ---------------------------------------------------------------------- -Boost Software License - Version 1.0 - August 17th, 2003 - -Permission is hereby granted, free of charge, to any person or -organization obtaining a copy of the software and accompanying -documentation covered by this license (the "Software") to use, -reproduce, display, distribute, execute, and transmit the Software, and -to prepare derivative works of the Software, and to permit third-parties -to whom the Software is furnished to do so, all subject to the -following: - -The copyright notices in the Software and this entire statement, -including the above license grant, this restriction and the following -disclaimer, must be included in all copies of the Software, in whole or -in part, and all derivative works of the Software, unless such copies or -derivative works are solely in the form of machine-executable object -code generated by a source language processor. - -THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS -OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF -MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE, TITLE AND NON- -INFRINGEMENT. IN NO EVENT SHALL THE COPYRIGHT HOLDERS OR ANYONE -DISTRIBUTING THE SOFTWARE BE LIABLE FOR ANY DAMAGES OR OTHER LIABILITY, -WHETHER IN CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN -CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE -SOFTWARE. - - -ATLAS ---------------------------------------------------------------------- -Upstream Authors: -R. Clint Whaley atlas@cs.utk.edu -Jack Dongarra atlas@cs.utk.edu - -Copyright: -(C) Copyright 1997-2008 All Rights Reserved - -Permission to use, copy, modify, and distribute this software and -its documentation for any purpose and without fee is hereby granted -provided that the above copyright notice appear in all copies and -that both the copyright notice and this permission notice appear in -supporting documentation. - -Neither the University of Tennessee nor the Author make any -representations about the suitability of this software for any -purpose. This software is provided ``as is'' without express or -implied warranty. - -On alpha architectures, the standard builds use K. GOTO's kernels -contributed to the ATLAS project under the LGPL. Therefore on these -platforms, the distributed prebuilt libraries, as well as any user -built library using this kernel, is licensed under the LGPL. - -On Debian GNU/Linux systems, the complete text of the GNU Lesser General -Public License can be found in `/usr/share/common-licenses/LGPL'. - - -BAMTOOLS (The MIT License) ---------------------------------------------------------------------- -Copyright (c) 2009-2010 Derek Barnett, Erik Garrison, Gabor Marth, Michael Stromberg +1.5. Applicable Law This Agreement shall be deemed to have been made in, and shall be construed pursuant to, the laws of the State of Delaware. The United Nations Convention on Contracts for the International Sale of Goods is specifically disclaimed. The courts of Santa Clara County, California shall have exclusive jurisdiction and venue over any dispute arising out of or relating to this Agreement. -Permission is hereby granted, free of charge, to any person obtaining a copy -of this software and associated documentation files (the "Software"), to deal -in the Software without restriction, including without limitation the rights -to use, copy, modify, merge, publish, distribute, sublicense, and/or sell -copies of the Software, and to permit persons to whom the Software is -furnished to do so, subject to the following conditions: +1.6. Disclaimer of Warranties and Limitations on Liability -The above copyright notice and this permission notice shall be included in -all copies or substantial portions of the Software. + 1.6.1. No Warranties TO THE MAXIMUM EXTENT PERMITTED BY APPLICABLE LAW, THE SOFTWARE IS PROVIDED "AS IS" AND NVIDIA AND ITS SUPPLIERS DISCLAIM ALL WARRANTIES, EITHER EXPRESS OR IMPLIED, INCLUDING, BUT NOT LIMITED TO, IMPLIED WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE, OR NONINFRINGEMENT. + + 1.6.2. No Liability for Consequential Damages TO THE MAXIMUM EXTENT PERMITTED BY APPLICABLE LAW, IN NO EVENT SHALL NVIDIA OR ITS SUPPLIERS BE LIABLE FOR ANY SPECIAL, INCIDENTAL, INDIRECT, OR CONSEQUENTIAL DAMAGES WHATSOEVER (INCLUDING, WITHOUT LIMITATION, DAMAGES FOR LOSS OF BUSINESS PROFITS, BUSINESS INTERRUPTION, LOSS OF BUSINESS INFORMATION, OR ANY OTHER PECUNIARY LOSS) ARISING OUT OF THE USE OF OR INABILITY TO USE THE SOFTWARE, EVEN IF NVIDIA HAS BEEN ADVISED OF THE POSSIBILITY OF SUCH DAMAGES. + + 1.6.3. No Support . NVIDIA has no obligation to support or to provide any updates of the Software. -THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR -IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, -FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE -AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER -LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, -OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN -THE SOFTWARE. +1.7. Miscellaneous + + 1.7.1. Feedback Notwithstanding any Non-Disclosure Agreement executed by and between the parties, the parties agree that in the event Licensee or NVIDIA provides Feedback (as defined below) to the other party on how to design, implement, or improve the SOFTWARE or Licensee's product(s) for use with the SOFTWARE, the following terms and conditions apply the Feedback: + 1.7.1.1. Exchange of Feedback Both parties agree that neither party has an obligation to give the other +party any suggestions, comments or other feedback, whether verbally or in written or source code form, relating to (i) the SOFTWARE; (ii) Licensee's products; (iii) Licensee's use of the SOFTWARE; or (iv) optimization/interoperability of Licensee's product with the SOFTWARE (collectively defined as "Feedback"). In the event either party provides Feedback to the other party, the party receiving the Feedback may use any Feedback that the other party voluntarily provides to improve the (i) SOFTWARE or other related NVIDIA technologies, respectively for the benefit of NVIDIA; or (ii) Licensee's product or other related Licensee technologies, respectively for the benefit of Licensee. Accordingly, if either party provides Feedback to the other party, both parties agree that the other party and its respective licensees may freely use, reproduce, license, distribute, and otherwise commercialize the Feedback in the (i) SOFTWARE or other related technologies; or (ii) Licensee's products or other related technologies, respectively, without the payment of any royalties or fees. -VCFLIB ---------------------------------------------------------------------- -Copyright (c) 2012 Erik Garrison + 1.7.1.2. Residual Rights Licensee agrees that NVIDIA shall be free to use any general knowledge, skills and experience, (including, but not limited to, ideas, concepts, know-how, or techniques) ("Residuals"), contained in the (i) Feedback provided by Licensee to NVIDIA; (ii) Licensee's products shared or disclosed to NVIDIA in connection with the Feedback; or (c) Licensee's confidential information voluntarily provided to NVIDIA in connection with the Feedback, which are retained in the memories of NVIDIA's employees, agents, or contractors who have had access to such Residuals. Subject to the terms and conditions of this Agreement, NVIDIA's employees, agents, or contractors shall not be prevented from using Residuals as part of such employee's, agent's or contractor's general knowledge, skills, experience, talent, and/or expertise. NVIDIA shall not have any obligation to limit or restrict the assignment of such employees, agents or contractors or to pay royalties for any work resulting from the use of Residuals. -Permission is hereby granted, free of charge, to any person obtaining a copy -of this software and associated documentation files (the "Software"), to deal -in the Software without restriction, including without limitation the rights -to use, copy, modify, merge, publish, distribute, sublicense, and/or sell -copies of the Software, and to permit persons to whom the Software is -furnished to do so, subject to the following conditions: + 1.7.1.3. Disclaimer of Warranty FEEDBACK FROM EITHER PARTY IS PROVIDED FOR THE OTHER PARTY'S USE "AS IS" AND BOTH PARTIES DISCLAIM ALL WARRANTIES, EXPRESS, IMPLIED AND STATUTORY INCLUDING, WITHOUT LIMITATION, THE IMPLIED WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE, OR NONINFRINGEMENT. BOTH PARTIES DO NOT REPRESENT OR WARRANT THAT THE FEEDBACK WILL MEET THE OTHER PARTY'S REQUIREMENTS OR THAT THE OPERATION OR IMPLEMENTATION OF THE FEEDBACK WILL BE UNINTERRUPTED OR ERROR-FREE. + 1.7.1.4. No Liability for Consequential Damages TO THE MAXIMUM EXTENT PERMITTED BY APPLICABLE LAW, IN NO EVENT SHALL EITHER PARTY OR ITS SUPPLIERS BE LIABLE FOR ANY SPECIAL, INCIDENTAL, INDIRECT, OR CONSEQUENTIAL DAMAGES WHATSOEVER (INCLUDING, WITHOUT LIMITATION, DAMAGES FOR LOSS OF BUSINESS PROFITS, BUSINESS INTERRUPTION, LOSS OF BUSINESS INFORMATION, OR ANY OTHER PECUNIARY LOSS) ARISING OUT OF THE USE OF OR INABILITY TO USE THE FEEDBACK, EVEN IF THE OTHER PARTY HAS BEEN ADVISED OF THE POSSIBILITY OF SUCH DAMAGES. -The above copyright notice and this permission notice shall be included in -all copies or substantial portions of the Software. + 1.7.2. Freedom of Action Licensee agrees that this Agreement is nonexclusive and NVIDIA may currently or in the future be developing software, other technology or confidential information internally, or receiving confidential information from other parties that maybe similar to the Feedback and Licensee's confidential information (as provided in Section 1.7.1.2 above), which may be provided to NVIDIA in connection with Feedback by Licensee. Accordingly, Licensee agrees that nothing in this Agreement will be construed as a representation or inference that NVIDIA will not develop, design, manufacture, acquire, market products, or have products developed, designed, manufactured, acquired, or marketed for NVIDIA, that compete with the Licensee's products or confidential information. -THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR -IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, -FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE -AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER -LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, -OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN -THE SOFTWARE. + 1.7.3. No Implied Licenses Under no circumstances should anything in this Agreement be construed as NVIDIA granting by implication, estoppel or otherwise, (i) a license to any NVIDIA product or technology other than the SOFTWARE; or (ii) any additional license rights for the SOFTWARE other than the licenses expressly granted in this Agreement. If any provision of this Agreement is inconsistent with, or cannot be fully enforced under, the law, such provision will be construed as limited to the extent necessary to be consistent with and fully enforceable under the law. This Agreement is the final, complete and exclusive agreement between the parties relating to the subject matter hereof, and supersedes all prior or contemporaneous understandings and agreements relating to such subject matter, whether oral or written. This Agreement may only be modified in writing signed by an authorized officer of NVIDIA. Licensee agrees that it will not ship, transfer or export the SOFTWARE into any country, or use the SOFTWARE in any manner, prohibited by the United States Bureau of Industry and Security or any export laws, restrictions or regulations. The parties agree that the following sections of the Agreement will survive the termination of the License: Section 1.2.1.4, Section 1.4, Section 1.5, Section 1.6, and Section 1.7. +1.8. Attachment A Redistributable Software In connection with Section 1.2.1.1 of this Agreement, the following files may be redistributed with software applications developed by Licensee, including certain variations of these files that have version number or architecture specific information NVIDIA CUDA Toolkit License Agreement www.nvidia.com End User License Agreements (EULA) DR-06739-001_v01_v8.0 | 9 embedded in the file name - as an example only, for release version 6.0 of the 64-bit Windows software, the file cudart64_60.dll is redistributable. +Component : CUDA Runtime Windows : cudart.dll, cudart_static.lib, cudadevrt.lib Mac OSX : libcudart.dylib, libcudart_static.a, libcudadevrt.a Linux : libcudart.so, libcudart_static.a, libcudadevrt.a Android : libcudart.so, libcudart_static.a, libcudadevrt.a Component : CUDA FFT Library Windows : cufft.dll, cufftw.dll Mac OSX : libcufft.dylib, libcufft_static.a, libcufftw.dylib, libcufftw_static.a Linux : libcufft.so, libcufft_static.a, libcufftw.so, libcufftw_static.a Android : libcufft.so, libcufft_static.a, libcufftw.so, libcufftw_static.a Component : CUDA BLAS Library Windows : cublas.dll, cublas_device.lib Mac OSX : libcublas.dylib, libcublas_static.a, libcublas_device.a Linux : libcublas.so, libcublas_static.a, libcublas_device.a Android : libcublas.so, libcublas_static.a, libcublas_device.a Component : NVIDIA "Drop-in" BLAS Library Windows : nvblas.dll Mac OSX : libnvblas.dylib Linux : libnvblas.so Component : CUDA Sparse Matrix Library Windows : cusparse.dll Mac OSX : libcusparse.dylib, libcusparse_static.a Linux : libcusparse.so, libcusparse_static.a Android : libcusparse.so, libcusparse_static.a Component : CUDA Linear Solver Library Windows : cusolver.dll Mac OSX : libcusolver.dylib, libcusolver_static.a Linux : libcusolver.so, libcusolver_static.a Android : libcusolver.so, libcusolver_static.a Component : CUDA Random Number Generation Library Windows : curand.dll Mac OSX : libcurand.dylib, libcurand_static.a Linux : libcurand.so, libcurand_static.a Android : libcurand.so, libcurand_static.a Component : NVIDIA Performance Primitives Library Windows : nppc.dll, nppi.dll, npps.dll Mac OSX : libnppc.dylib, libnppi.dylib, libnpps.dylib, libnppc_static.a, libnpps_static.a, libnppi_static.a Linux : libnppc.so, libnppi.so, libnpps.so, libnppc_static.a, libnpps_static.a, libnppi_static.a Android : libnppc.so, libnppi.so, libnpps.so, libnppc_static.a, libnpps_static.a, libnppi_static.a Component : Internal common library required for statically linking to cuBLAS, cuSPARSE, cuFFT, cuRAND and NPP Mac OSX : libculibos.a Linux : libculibos.a Component : NVIDIA Runtime Compilation Library Windows : nvrtc.dll, nvrtc-builtins.dll Mac OSX : libnvrtc.dylib, libnvrtc-builtins.dylib Linux : libnvrtc.so, libnvrtc-builtins.so Component : NVIDIA Optimizing Compiler Library Windows : nvvm.dll Mac OSX : libnvvm.dylib Linux : libnvvm.so Component : NVIDIA Common Device Math Functions Library Windows : libdevice.compute_20.bc, libdevice.compute_30.bc, libdevice.compute_35.bc Mac OSX : libdevice.compute_20.bc, libdevice.compute_30.bc, libdevice.compute_35.bc Linux : libdevice.compute_20.bc, libdevice.compute_30.bc, libdevice.compute_35.bc Component : CUDA Occupancy Calculation Header Library All : cuda_occupancy.h Component : Profiling Tools Interface Library Windows : cupti.dll Mac OSX : libcupti.dylib Linux : libcupti.so -FREEBAYES v0.9.8 ---------------------------------------------------------------------- -Copyright (c) 2010 Erik Garrison, Gabor Marth +1.9. Attachment B Additional Licensing Obligations The following third party components included in the SOFTWARE are licensed to Licensee pursuant to the following terms and conditions: + -Permission is hereby granted, free of charge, to any person obtaining a copy -of this software and associated documentation files (the "Software"), to deal -in the Software without restriction, including without limitation the rights -to use, copy, modify, merge, publish, distribute, sublicense, and/or sell -copies of the Software, and to permit persons to whom the Software is -furnished to do so, subject to the following conditions: +1. Licensee's use of the GDB third party component is subject to the terms and conditions of GNU GPL v3: This product includes copyrighted third-party software licensed under the terms of the GNU General Public License v3 ("GPL v3"). All third-party software packages are copyright by their respective authors. GPL v3 terms and conditions are hereby incorporated into the Agreement by this reference: http://www.gnu.org/licenses/gpl.txt Consistent with these licensing requirements, the software listed below is provided under the terms of the specified open source software licenses. To obtain source code for software provided under licenses that require redistribution of source code, including the GNU General Public License (GPL) and GNU Lesser General Public License (LGPL), contact oss-requests@nvidia.com. This offer is valid for a period of three (3) years from the date of the distribution of this product by NVIDIA CORPORATION. Component License CUDA-GDB GPL v3 -The above copyright notice and this permission notice shall be included in -all copies or substantial portions of the Software. +2. Licensee represents and warrants that any and all third party licensing and/or royalty payment obligations in connection with Licensee's use of the H.264 video codecs are solely the responsibility of Licensee. -THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR -IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, -FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE -AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER -LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, -OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN -THE SOFTWARE. +3. Licensee's use of the Thrust library is subject to the terms and conditions of the Apache License Version 2.0. All third-party software packages are copyright by their respective authors. Apache License Version 2.0 terms and conditions are hereby incorporated into the Agreement by this reference. http://www.apache.org/licenses/ LICENSE-2.0.html. In addition, Licensee acknowledges the following notice: Thrust includes source code from the Boost Iterator, Tuple, System, and Random Number libraries. Boost Software License - Version 1.0 - August 17th, 2003 . . . . Permission is hereby granted, free of charge, to any person or organization obtaining a copy of the software and accompanying documentation covered by this license (the "Software") to use, reproduce, display, distribute, execute, and transmit the Software, and to prepare derivative works of the Software, and to permit third-parties to whom the Software is furnished to do so, all subject to the following: The copyright notices in the Software and this entire statement, including the above license grant, this restriction and the following disclaimer, must be included in all copies of the Software, in whole or in part, and all derivative works of the Software, unless such copies or derivative works are solely in the form of machine-executable object code generated by a source language processor. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE, TITLE AND NON-INFRINGEMENT. IN NO EVENT SHALL THE COPYRIGHT HOLDERS OR ANYONE DISTRIBUTING THE SOFTWARE BE LIABLE FOR ANY DAMAGES OR OTHER LIABILITY, WHETHER IN CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE. +4. Licensee's use of the LLVM third party component is subject to the following terms and conditions: + + LLVM Release License + + University of Illinois/NCSA Open Source License Copyright (c) 2003-2010 University of Illinois at Urbana-Champaign. All rights reserved. Developed by: LLVM Team University of Illinois at Urbana-Champaign http://llvm.org Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal with the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: * Redistributions of source code must retain the above copyright notice, this list of conditions and the following disclaimers. * Redistributions in binary form must reproduce the above copyright notice, this list of conditions and the following disclaimers in the documentation and/or other materials provided with the distribution. * Neither the names of the LLVM Team, University of Illinois at Urbana- Champaign, nor the names of its contributors may be used to endorse or promote products derived from this Software without specific prior written permission. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE CONTRIBUTORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS WITH THE SOFTWARE. +5. Licensee's use of the PCRE third party component is subject to the following terms and conditions: + PCRE LICENCE + + PCRE is a library of functions to support regular expressions whose syntax and semantics are as close as possible to those of the Perl 5 language. Release 8 of PCRE is distributed under the terms of the "BSD" licence, as specified below. The documentation for PCRE, supplied in the "doc" directory, is distributed under the same terms as the software itself. The basic library functions are written in C and are freestanding. Also included in the distribution is a set of C++ wrapper functions, and a justin-time compiler that can be used to optimize pattern matching. These are both optional features that can be omitted when the library is built. + + + THE BASIC LIBRARY FUNCTIONS + + Written by: Philip Hazel + Email local part: ph10 + Email domain: cam.ac.uk University of Cambridge Computing Service, Cambridge, England. + Copyright (c) 1997-2012 University of Cambridge All rights reserved. + + PCRE JUST-IN-TIME COMPILATION SUPPORT + Written by: Zoltan Herczeg + Email local part: hzmester + Emain domain: freemail.hu + Copyright(c) 2010-2012 Zoltan Herczeg All rights reserved. + + STACK-LESS JUST-IN-TIME COMPILER -===================================================================== -3. GPL v2 -===================================================================== -GNU GENERAL PUBLIC LICENSE -Version 2, June 1991 -Copyright (C) 1989, 1991 Free Software Foundation, Inc. -51 Franklin Street, Fifth Floor, Boston, MA 02110-1301, USA + Written by: Zoltan Herczeg Email local part: hzmester Emain domain: freemail.hu Copyright(c) 2009-2012 Zoltan Herczeg All rights reserved. + + THE C++ WRAPPER FUNCTIONS + + Contributed by: Google Inc. Copyright (c) 2007-2012, Google Inc. 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Some of the cuBLAS library routines were written by or derived from code written by Vasily Volkov and are subject to the Modified Berkeley Software Distribution License as follows: Copyright (c) 2007-2009, Regents of the University of California All rights reserved. Redistribution and use in source and binary forms, with or without modification, are permitted provided that the following conditions are met: * Redistributions of source code must retain the above copyright notice, this list of conditions and the following disclaimer. * Redistributions in binary form must reproduce the above copyright notice, this list of conditions and the following disclaimer in the documentation and/or other materials provided with the distribution. * Neither the name of the University of California, Berkeley nor the names of its contributors may be used to endorse or promote products derived from this software without specific prior written permission. THIS SOFTWARE IS PROVIDED BY THE AUTHOR "AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE AUTHOR BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE. + +7. Some of the cuBLAS library routines were written by or derived from code written by Davide Barbieri and are subject to the Modified Berkeley Software Distribution License as follows: Copyright (c) 2008-2009 Davide Barbieri @ University of Rome Tor Vergata. All rights reserved. Redistribution and use in source and binary forms, with or without modification, are permitted provided that the following conditions are met: * Redistributions of source code must retain the above copyright notice, this list of conditions and the following disclaimer. * Redistributions in binary form must reproduce the above copyright notice, this list of conditions and the following disclaimer in the documentation and/or other materials provided with the distribution. * The name of the author may not be used to endorse or promote products derived from this software without specific prior written permission. THIS SOFTWARE IS PROVIDED BY THE AUTHOR "AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE DISCLAIMED. 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Redistribution and use in source and binary forms, with or without modification, are permitted provided that the following conditions are met: * Redistributions of source code must retain the above copyright notice, this list of conditions and the following disclaimer. * Redistributions in binary form must reproduce the above copyright notice, this list of conditions and the following disclaimer listed in this license in the documentation and/or other materials provided with the distribution. * Neither the name of the copyright holders nor the names of its contributors may be used to endorse or promote products derived from this software without specific prior written permission. THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS "AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE COPYRIGHT OWNER OR CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE. + +9. Some of the cuBLAS library routines were written by or derived from code written by Jonathan Hogg and are subject to the Modified Berkeley Software Distribution License as follows: Copyright (c) 2012, The Science and Technology Facilities Council (STFC). All rights reserved. Redistribution and use in source and binary forms, with or without modification, are permitted provided that the following conditions are met: * Redistributions of source code must retain the above copyright notice, this list of conditions and the following disclaimer. * Redistributions in binary form must reproduce the above copyright notice, this list of conditions and the following disclaimer in the documentation and/or other materials provided with the distribution. * Neither the name of the STFC nor the names of its contributors may be used to endorse or promote products derived from this software without specific prior written permission. THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS "AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE STFC BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE. + +10. Some of the cuBLAS library routines were written by or derived from code written by Ahmad M. Abdelfattah, David Keyes, and Hatem Ltaief, and are subject to the Apache License, Version 2.0, as follows: -- (C) Copyright 2013 King Abdullah University of Science and Technology Authors: Ahmad Abdelfattah (ahmad.ahmad@kaust.edu.sa) David Keyes (david.keyes@kaust.edu.sa) Hatem Ltaief (hatem.ltaief@kaust.edu.sa) Redistribution and use in source and binary forms, with or without modification, are permitted provided that the following conditions are met: * Redistributions of source code must retain the above copyright notice, this list of conditions and the following disclaimer. * Redistributions in binary form must reproduce the above copyright notice, this list of conditions and the following disclaimer in the documentation and/or other materials provided with the distribution. * Neither the name of the King Abdullah University of Science and Technology nor the names of its contributors may be used to endorse or promote products derived from this software without specific prior written permission. THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS ``AS IS'' AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE COPYRIGHT HOLDERS OR CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE. + +11. Some of the cuSPARSE library routines were written by or derived from code written by Li-Wen Chang and are subject to the NCSA Open Source License as follows: Copyright (c) 2012, University of Illinois. All rights reserved. Developed by: IMPACT Group, University of Illinois, http:// impact.crhc.illinois.edu Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal with the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: * Redistributions of source code must retain the above copyright notice, this list of conditions and the following disclaimer. * Redistributions in binary form must reproduce the above copyright notice, this list of conditions and the following disclaimers in the documentation and/or other materials provided with the distribution. * Neither the names of IMPACT Group, University of Illinois, nor the names of its contributors may be used to endorse or promote products derived from this Software without specific prior written permission. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE CONTRIBUTORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS WITH THE SOFTWARE. + +12. Some of the cuRAND library routines were written by or derived from code written by Mutsuo Saito and Makoto Matsumoto and are subject to the following license: Copyright (c) 2009, 2010 Mutsuo Saito, Makoto Matsumoto and Hiroshima University. All rights reserved. Copyright (c) 2011 Mutsuo Saito, Makoto Matsumoto, Hiroshima University and University of Tokyo. All rights reserved. Redistribution and use in source and binary forms, with or without modification, are permitted provided that the following conditions are met: * Redistributions of source code must retain the above copyright notice, this list of conditions and the following disclaimer. * Redistributions in binary form must reproduce the above copyright notice, this list of conditions and the following disclaimer in the documentation and/or other materials provided with the distribution. * Neither the name of the Hiroshima University nor the names of its contributors may be used to endorse or promote products derived from this software without specific prior written permission. THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS "AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE COPYRIGHT OWNER OR CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE. + +13. Some of the cuRAND library routines were derived from code developed by D. E. Shaw Research and are subject to the following license: Copyright 2010-2011, D. E. Shaw Research. All rights reserved. Redistribution and use in source and binary forms, with or without modification, are permitted provided that the following conditions are met: * Redistributions of source code must retain the above copyright notice, this list of conditions, and the following disclaimer. * Redistributions in binary form must reproduce the above copyright notice, this list of conditions, and the following disclaimer in the documentation and/or other materials provided with the distribution. * Neither the name of D. E. Shaw Research nor the names of its contributors may be used to endorse or promote products derived from this software without specific prior written permission. THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS "AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE COPYRIGHT OWNER OR CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE. + +14. Licensee's use of the lz4 third party component is subject to the following terms and conditions: Copyright (C) 2011-2013, Yann Collet. BSD 2-Clause License (http://www.opensource.org/licenses/bsd-license.php) Redistribution and use in source and binary forms, with or without modification, are permitted provided that the following conditions are met: * Redistributions of source code must retain the above copyright notice, this list of conditions and the following disclaimer. * Redistributions in binary form must reproduce the above copyright notice, this list of conditions and the following disclaimer in the documentation and/or other materials provided with the distribution. THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS "AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE COPYRIGHT OWNER OR CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE. + +15. The NPP library uses code from the Boost Math Toolkit, and is subject to the following license: Boost Software License - Version 1.0 - August 17th, 2003 . . . . Permission is hereby granted, free of charge, to any person or organization obtaining a copy of the software and accompanying documentation covered by this license (the "Software") to use, reproduce, display, distribute, execute, and transmit the Software, and to prepare derivative works of the Software, and to permit third-parties to whom the Software is furnished to do so, all subject to the following: The copyright notices in the Software and this entire statement, including the above license grant, this restriction and the following disclaimer, must be included in all copies of the Software, in whole or in part, and all derivative works of the Software, unless such copies or derivative works are solely in the form of machine-executable object code generated by a source language processor. 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IN NO EVENT SHALL THE COPYRIGHT HOLDERS OR ANYONE DISTRIBUTING THE SOFTWARE BE LIABLE FOR ANY DAMAGES OR OTHER LIABILITY, WHETHER IN CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE. + +Ansible +------------------------------------------------------------- GNU GENERAL PUBLIC LICENSE + Version 3, 29 June 2007 + Copyright (c) 2007 Free Software Foundation, Inc. + Everyone is permitted to copy and distribute verbatim copies of this license document, but changing it is not allowed. + Preamble + The GNU General Public License is a free, copyleft license for software and other kinds of works. + The licenses for most software and other practical works are designed to take away your freedom to share and change the works. By contrast, the GNU General Public License is intended to guarantee your freedom to share and change all versions of a program--to @@ -495,6 +655,7 @@ Public Licenses are designed to make sure that you have the freedom to distribut of free software (and charge for them if you wish), that you receive source code or can get it if you want it, that you can change the software or use pieces of it in new free programs, and that you know you can do these things. + To protect your rights, we need to prevent others from denying you these rights or asking you to surrender the rights. Therefore, you have certain responsibilities if you distribute copies of the software, or if you modify it: responsibilities to respect the freedom of @@ -503,13 +664,16 @@ For example, if you distribute copies of such a program, whether gratis or for a must pass on to the recipients the same freedoms that you received. You must make sure that they, too, receive or can get the source code. And you must show them these terms so they know their rights. -Developers that use the GNU GPL protect your rights with two steps: (1) assert copyright -on the software, and (2) offer you this License giving you legal permission to copy, -distribute and/or modify it. + +Developers that use the GNU GPL protect your rights with two steps: + + (1) assert copyright on the software, and + (2) offer you this License giving you legal permission to copy, distribute and/or modify it. For the developers' and authors' protection, the GPL clearly explains that there is no warranty for this free software. For both users' and authors' sake, the GPL requires that modified versions be marked as changed, so that their problems will not be attributed erroneously to authors of previous versions. + Some devices are designed to deny users access to install or run modified versions of the software inside them, although the manufacturer can do so. This is fundamentally incompatible with the aim of protecting users' freedom to change the software. The @@ -518,13 +682,16 @@ which is precisely where it is most unacceptable. Therefore, we have designed th of the GPL to prohibit the practice for those products. If such problems arise substantially in other domains, we stand ready to extend this provision to those domains in future versions of the GPL, as needed to protect the freedom of users. + Finally, every program is threatened constantly by software patents. States should not allow patents to restrict development and use of software on general-purpose computers, but in those that do, we wish to avoid the special danger that patents applied to a free program could make it effectively proprietary. To prevent this, the GPL assures that patents cannot be used to render the program non-free. The precise terms and conditions for copying, distribution and modification follow. + TERMS AND CONDITIONS + 0. Definitions. "This License" refers to version 3 of the GNU General Public License. "Copyright" also means copyright-like laws that apply to other kinds of works, such as @@ -551,6 +718,7 @@ copyright notice, and (2) tells the user that there is no warranty for the work the extent that warranties are provided), that licensees may convey the work under this License, and how to view a copy of this License. If the interface presents a list of user commands or options, such as a menu, a prominent item in the list meets this criterion. + 1. Source Code. The "source code" for a work means the preferred form of the work for making modifications to it. "Object code" means any non-source form of a work. @@ -579,6 +747,7 @@ parts of the work. The Corresponding Source need not include anything that users can regenerate automatically from other parts of the Corresponding Source. The Corresponding Source for a work in source code form is that same work. + 2. Basic Permissions. All rights granted under this License are granted for the term of copyright on the Program, and are irrevocable provided the stated conditions are met. This License @@ -596,6 +765,7 @@ exclusively on your behalf, under your direction and control, on terms that proh from making any copies of your copyrighted material outside their relationship with you. Conveying under any other circumstances is permitted solely under the conditions stated below. Sublicensing is not allowed; section 10 makes it unnecessary. + 3. Protecting Users' Legal Rights From Anti-Circumvention Law. No covered work shall be deemed part of an effective technological measure under any applicable law fulfilling obligations under article 11 of the WIPO copyright treaty @@ -606,6 +776,7 @@ technological measures to the extent such circumvention is effected by exercisin under this License with respect to the covered work, and you disclaim any intention to limit operation or modification of the work as a means of enforcing, against the work's users, your or third parties' legal rights to forbid circumvention of technological measures. + 4. Conveying Verbatim Copies. You may convey verbatim copies of the Program's source code as you receive it, in any medium, provided that you conspicuously and appropriately publish on each copy an @@ -615,22 +786,23 @@ of the absence of any warranty; and give all recipients a copy of this License a the Program. You may charge any price or no price for each copy that you convey, and you may offer support or warranty protection for a fee. + 5. Conveying Modified Source Versions. You may convey a work based on the Program, or the modifications to produce it from the Program, in the form of source code under the terms of section 4, provided that you also meet all of these conditions: -* a) The work must carry prominent notices stating that you modified it, and giving + a) The work must carry prominent notices stating that you modified it, and giving a relevant date. -* b) The work must carry prominent notices stating that it is released under this + b) The work must carry prominent notices stating that it is released under this License and any conditions added under section 7. This requirement modifies the requirement in section 4 to "keep intact all notices". -* c) You must license the entire work, as a whole, under this License to anyone who + c) You must license the entire work, as a whole, under this License to anyone who comes into possession of a copy. This License will therefore apply, along with any applicable section 7 additional terms, to the whole of the work, and all its parts, regardless of how they are packaged. This License gives no permission to license the work in any other way, but it does not invalidate such permission if you have separately received it. -* d) If the work has interactive user interfaces, each must display Appropriate Legal + d) If the work has interactive user interfaces, each must display Appropriate Legal Notices; however, if the Program has interactive interfaces that do not display Appropriate Legal Notices, your work need not make them do so. A compilation of a covered work with other separate and independent works, which are @@ -640,14 +812,15 @@ called an "aggregate" if the compilation and its resulting copyright are not use the access or legal rights of the compilation's users beyond what the individual works permit. 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You may convey a covered work in object code form under the terms of sections 4 and 5, provided that you also convey the machine-readable Corresponding Source under the terms of this License, in one of these ways: -* a) Convey the object code in, or embodied in, a physical product (including a + a) Convey the object code in, or embodied in, a physical product (including a physical distribution medium), accompanied by the Corresponding Source fixed on a durable physical medium customarily used for software interchange. -* b) Convey the object code in, or embodied in, a physical product (including a + b) Convey the object code in, or embodied in, a physical product (including a physical distribution medium), accompanied by a written offer, valid for at least three years and valid for as long as you offer spare parts or customer support for that product model, to give anyone who possesses the object code either (1) a @@ -656,11 +829,11 @@ covered by this License, on a durable physical medium customarily used for software interchange, for a price no more than your reasonable cost of physically performing this conveying of source, or (2) access to copy the Corresponding Source from a network server at no charge. -* c) Convey individual copies of the object code with a copy of the written offer to + c) Convey individual copies of the object code with a copy of the written offer to provide the Corresponding Source. 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"Additional permissions" are terms that supplement the terms of this License by making exceptions from one or more of its conditions. Additional permissions that are applicable @@ -726,19 +900,19 @@ which you have or can give appropriate copyright permission. 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Ancillary propagation of a covered work occurring solely as a consequence of @@ -781,6 +957,7 @@ However, nothing other than this License grants you permission to propagate or m any covered work. These actions infringe copyright if you do not accept this License. Therefore, by modifying or propagating a covered work, you indicate your acceptance of this License to do so. + 10. Automatic Licensing of Downstream Recipients. Each time you convey a covered work, the recipient automatically receives a license from the original licensors, to run, modify and propagate that work, subject to this License. @@ -798,6 +975,7 @@ other charge for exercise of rights granted under this License, and you may not litigation (including a cross-claim or counterclaim in a lawsuit) alleging that any patent claim is infringed by making, using, selling, offering for sale, or importing the Program or any portion of it. + 11. Patents. A "contributor" is a copyright holder who authorizes use under this License of the Program or a work on which the Program is based. The work thus licensed is called the @@ -847,6 +1025,7 @@ that patent license was granted, prior to 28 March 2007. Nothing in this License shall be construed as excluding or limiting any implied license or other defenses to infringement that may otherwise be available to you under applicable patent law. + 12. No Surrender of Others' Freedom. If conditions are imposed on you (whether by court order, agreement or otherwise) that contradict the conditions of this License, they do not excuse you from the conditions of @@ -856,6 +1035,7 @@ you may not convey it at all. For example, if you agree to terms that obligate y collect a royalty for further conveying from those to whom you convey the Program, the only way you could satisfy both those terms and this License would be to refrain entirely from conveying the Program. + 13. Use with the GNU Affero General Public License. Notwithstanding any other provision of this License, you have permission to link or combine any covered work with a work licensed under version 3 of the GNU Affero @@ -863,6 +1043,7 @@ General Public License into a single combined work, and to convey the resulting The terms of this License will continue to apply to the part which is the covered work, but the special requirements of the GNU Affero General Public License, section 13, concerning interaction through a network will apply to the combination as such. + 14. Revised Versions of this License. The Free Software Foundation may publish revised and/or new versions of the GNU General Public License from time to time. Such new versions will be similar in spirit to the @@ -879,6 +1060,7 @@ version permanently authorizes you to choose that version for the Program. Later license versions may give you additional or different permissions. 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And you must show them these terms so they know their rights. + +We protect your rights with two steps: (1) copyright the software, and (2) offer you this license which gives you legal permission to copy, distribute and/or modify the software. + +Also, for each author's protection and ours, we want to make certain that everyone understands that there is no warranty for this free software. If the software is modified by someone else and passed on, we want its recipients to know that what they have is not the original, so that any problems introduced by others will not reflect on the original authors' reputations. + +Finally, any free program is threatened constantly by software patents. We wish to avoid the danger that redistributors of a free program will individually obtain patent licenses, in effect making the program proprietary. To prevent this, we have made it clear that any patent must be licensed for everyone's free use or not licensed at all. + +The precise terms and conditions for copying, distribution and modification follow. + +TERMS AND CONDITIONS FOR COPYING, DISTRIBUTION AND MODIFICATION +------------------------------------------------------------- + +0. This License applies to any program or other work which contains a notice placed by the copyright holder saying it may be distributed under the terms of this General Public License. The "Program", below, refers to any such program or work, and a "work based on the Program" means either the Program or any derivative work under copyright law: that is to say, a work containing the Program or a portion of it, either verbatim or with modifications and/or translated into another language. (Hereinafter, translation is included without limitation in the term "modification".) Each licensee is addressed as "you". +Activities other than copying, distribution and modification are not covered by this License; they are outside its scope. The act of running the Program is not restricted, and the output from the Program is covered only if its contents constitute a work based on the Program (independent of having been made by running the Program). Whether that is true depends on what the Program does. + +1. You may copy and distribute verbatim copies of the Program's source code as you receive it, in any medium, provided that you conspicuously and appropriately publish on each copy an appropriate copyright notice and disclaimer of warranty; keep intact all the notices that refer to this License and to the absence of any warranty; and give any other recipients of the Program a copy of this License along with the Program. +You may charge a fee for the physical act of transferring a copy, and you may at your option offer warranty protection in exchange for a fee. + +2. You may modify your copy or copies of the Program or any portion of it, thus forming a work based on the Program, and copy and distribute such modifications or work under the terms of Section 1 above, provided that you also meet all of these conditions: + + a) You must cause the modified files to carry prominent notices stating that you changed the files and the date of any change. + b) You must cause any work that you distribute or publish, that in whole or in part contains or is derived from the Program or any part thereof, to be licensed as a whole at no charge to all third parties under the terms of this License. + c) If the modified program normally reads commands interactively when run, you must cause it, when started running for such interactive use in the most ordinary way, to print or display an announcement including an appropriate copyright notice and a notice that there is no warranty (or else, saying that you provide a warranty) and that users may redistribute the program under these conditions, and telling the user how to view a copy of this License. (Exception: if the Program itself is interactive but does not normally print such an announcement, your work based on the Program is not required to print an announcement.) + + These requirements apply to the modified work as a whole. If identifiable sections of that work are not derived from the Program, and can be reasonably considered independent and separate works in themselves, then this License, and its terms, do not apply to those sections when you distribute them as separate works. But when you distribute the same sections as part of a whole which is a work based on the Program, the distribution of the whole must be on the terms of this License, whose permissions for other licensees extend to the entire whole, and thus to each and every part regardless of who wrote it. + + Thus, it is not the intent of this section to claim rights or contest your rights to work written entirely by you; rather, the intent is to exercise the right to control the distribution of derivative or collective works based on the Program. + + In addition, mere aggregation of another work not based on the Program with the Program (or with a work based on the Program) on a volume of a storage or distribution medium does not bring the other work under the scope of this License. + +3. You may copy and distribute the Program (or a work based on it, under Section 2) in object code or executable form under the terms of Sections 1 and 2 above provided that you also do one of the following: + + a) Accompany it with the complete corresponding machine-readable source code, which must be distributed under the terms of Sections 1 and 2 above on a medium customarily used for software interchange; or, + b) Accompany it with a written offer, valid for at least three years, to give any third party, for a charge no more than your cost of physically performing source distribution, a complete machine-readable copy of the corresponding source code, to be distributed under the terms of Sections 1 and 2 above on a medium customarily used for software interchange; or, + c) Accompany it with the information you received as to the offer to distribute corresponding source code. (This alternative is allowed only for noncommercial distribution and only if you received the program in object code or executable form with such an offer, in accord with Subsection b above.) +The source code for a work means the preferred form of the work for making modifications to it. For an executable work, complete source code means all the source code for all modules it contains, plus any associated interface definition files, plus the scripts used to control compilation and installation of the executable. However, as a special exception, the source code distributed need not include anything that is normally distributed (in either source or binary form) with the major components (compiler, kernel, and so on) of the operating system on which the executable runs, unless that component itself accompanies the executable. +If distribution of executable or object code is made by offering access to copy from a designated place, then offering equivalent access to copy the source code from the same place counts as distribution of the source code, even though third parties are not compelled to copy the source along with the object code. + +4. You may not copy, modify, sublicense, or distribute the Program except as expressly provided under this License. Any attempt otherwise to copy, modify, sublicense or distribute the Program is void, and will automatically terminate your rights under this License. However, parties who have received copies, or rights, from you under this License will not have their licenses terminated so long as such parties remain in full compliance. + +5. You are not required to accept this License, since you have not signed it. However, nothing else grants you permission to modify or distribute the Program or its derivative works. These actions are prohibited by law if you do not accept this License. Therefore, by modifying or distributing the Program (or any work based on the Program), you indicate your acceptance of this License to do so, and all its terms and conditions for copying, distributing or modifying the Program or works based on it. + +6. Each time you redistribute the Program (or any work based on the Program), the recipient automatically receives a license from the original licensor to copy, distribute or modify the Program subject to these terms and conditions. You may not impose any further restrictions on the recipients' exercise of the rights granted herein. You are not responsible for enforcing compliance by third parties to this License. + +7. If, as a consequence of a court judgment or allegation of patent infringement or for any other reason (not limited to patent issues), conditions are imposed on you (whether by court order, agreement or otherwise) that contradict the conditions of this License, they do not excuse you from the conditions of this License. If you cannot distribute so as to satisfy simultaneously your obligations under this License and any other pertinent obligations, then as a consequence you may not distribute the Program at all. For example, if a patent license would not permit royalty-free redistribution of the Program by all those who receive copies directly or indirectly through you, then the only way you could satisfy both it and this License would be to refrain entirely from distribution of the Program. +If any portion of this section is held invalid or unenforceable under any particular circumstance, the balance of the section is intended to apply and the section as a whole is intended to apply in other circumstances. + + It is not the purpose of this section to induce you to infringe any patents or other property right claims or to contest validity of any such claims; this section has the sole purpose of protecting the integrity of the free software distribution system, which is implemented by public license practices. Many people have made generous contributions to the wide range of software distributed through that system in reliance on consistent application of that system; it is up to the author/donor to decide if he or she is willing to distribute software through any other system and a licensee cannot impose that choice. +This section is intended to make thoroughly clear what is believed to be a consequence of the rest of this License. + +8. If the distribution and/or use of the Program is restricted in certain countries either by patents or by copyrighted interfaces, the original copyright holder who places the Program under this License may add an explicit geographical distribution limitation excluding those countries, so that distribution is permitted only in or among countries not thus excluded. In such case, this License incorporates the limitation as if written in the body of this License. + +9. The Free Software Foundation may publish revised and/or new versions of the General Public License from time to time. Such new versions will be similar in spirit to the present version, but may differ in detail to address new problems or concerns. +Each version is given a distinguishing version number. If the Program specifies a version number of this License which applies to it and "any later version", you have the option of following the terms and conditions either of that version or of any later version published by the Free Software Foundation. If the Program does not specify a version number of this License, you may choose any version ever published by the Free Software Foundation. + +10. If you wish to incorporate parts of the Program into other free programs whose distribution conditions are different, write to the author to ask for permission. For software which is copyrighted by the Free Software Foundation, write to the Free Software Foundation; we sometimes make exceptions for this. Our decision will be guided by the two goals of preserving the free status of all derivatives of our free software and of promoting the sharing and reuse of software generally. +NO WARRANTY + +11. BECAUSE THE PROGRAM IS LICENSED FREE OF CHARGE, THERE IS NO WARRANTY FOR THE PROGRAM, TO THE EXTENT PERMITTED BY APPLICABLE LAW. EXCEPT WHEN OTHERWISE STATED IN WRITING THE COPYRIGHT HOLDERS AND/OR OTHER PARTIES PROVIDE THE PROGRAM "AS IS" WITHOUT WARRANTY OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE. THE ENTIRE RISK AS TO THE QUALITY AND PERFORMANCE OF THE PROGRAM IS WITH YOU. SHOULD THE PROGRAM PROVE DEFECTIVE, YOU ASSUME THE COST OF ALL NECESSARY SERVICING, REPAIR OR CORRECTION. + +12. IN NO EVENT UNLESS REQUIRED BY APPLICABLE LAW OR AGREED TO IN WRITING WILL ANY COPYRIGHT HOLDER, OR ANY OTHER PARTY WHO MAY MODIFY AND/OR REDISTRIBUTE THE PROGRAM AS PERMITTED ABOVE, BE LIABLE TO YOU FOR DAMAGES, INCLUDING ANY GENERAL, SPECIAL, INCIDENTAL OR CONSEQUENTIAL DAMAGES ARISING OUT OF THE USE OR INABILITY TO USE THE PROGRAM (INCLUDING BUT NOT LIMITED TO LOSS OF DATA OR DATA BEING RENDERED INACCURATE OR LOSSES SUSTAINED BY YOU OR THIRD PARTIES OR A FAILURE OF THE PROGRAM TO OPERATE WITH ANY OTHER PROGRAMS), EVEN IF SUCH HOLDER OR OTHER PARTY HAS BEEN ADVISED OF THE POSSIBILITY OF SUCH DAMAGES. diff --git a/buildTools/build.sh b/buildTools/build.sh index 15cc7590..aee0f01d 100755 --- a/buildTools/build.sh +++ b/buildTools/build.sh @@ -82,6 +82,14 @@ for MODULE in $MODULES; do fi done; +# if the environmental variable is set to create a repository, we will move all of the packages into that repository and +# index them so aptitude can read them in as a repository +if [ ! -z ${MAKE_REPO_DIRECTORY} ]; then + mkdir -p ${BUILD_ROOT}/build/repo + find ${BUILD_ROOT}/build -type f -iname "*.deb" ! -path "$BUILD_ROOT/build/*" -exec mv {} ${BUILD_ROOT}/build/repo \; + cd ${BUILD_ROOT}/build/repo && dpkg-scanpackages -m ./ > ${BUILD_ROOT}/build/repo/Packages +fi + if [ $ERR != 0 ]; then echo -e $ERRMSG echo "FAILURES: $ERR modules failed to build." @@ -89,3 +97,4 @@ if [ $ERR != 0 ]; then else echo "SUCCESS: All modules built." fi + diff --git a/buildTools/cmake/CMakeLists.compiler.txt b/buildTools/cmake/CMakeLists.compiler.txt index 83c84616..028ace3d 100644 --- a/buildTools/cmake/CMakeLists.compiler.txt +++ b/buildTools/cmake/CMakeLists.compiler.txt @@ -16,8 +16,9 @@ endif(LSB_RELEASE_COMMAND) # Common compiler flags # char is per default signed on x86_64 systems but unsigned on ARM, use signed-char -if (${LSB_RELEASE_CODENAME} STREQUAL "trusty") - set(ION_COMMON_FLAGS "${ION_COMMON_FLAGS} -Wall -Wextra -Werror -fPIC -fsigned-char") +if (${LSB_RELEASE_CODENAME} STREQUAL "trusty" OR + ${LSB_RELEASE_CODENAME} STREQUAL "xenial") + set(ION_COMMON_FLAGS "${ION_COMMON_FLAGS} -Wall -Wextra -Werror -fPIC -fsigned-char -Wno-deprecated-declarations") else() set(ION_COMMON_FLAGS "${ION_COMMON_FLAGS} -Wall -Wextra -fPIC -fsigned-char") endif() @@ -78,13 +79,14 @@ set(CUDA_PROPAGATE_HOST_FLAGS OFF) ## NOTE: -O2 enables strict-aliasing, and code is not clean SET( CUDA_NVCC_FLAGS ${CUDA_NVCC_FLAGS} - "-std=c++11" "-O2" "--compiler-options=-fno-strict-aliasing" "-g" "--use_fast_math" + "-D_MWAITXINTRIN_H_INCLUDED" "-D_FORCE_INLINES" "-std=c++11" "-O2" "--compiler-options=-fno-strict-aliasing" "-g" "--use_fast_math" "-gencode=arch=compute_20,code=sm_20" "-gencode=arch=compute_30,code=sm_30" "-gencode=arch=compute_35,code=sm_35" "-gencode=arch=compute_50,code=sm_50" "-gencode=arch=compute_52,code=sm_52" "-gencode=arch=compute_52,code=compute_52" + "-gencode=arch=compute_61,code=sm_61" "-maxrregcount=48" ) ## If user set CXX on command line, make sure NVCC respects it diff --git a/buildTools/cmake/CMakeLists.dependencies.txt b/buildTools/cmake/CMakeLists.dependencies.txt index f821d2cc..0ed70f09 100644 --- a/buildTools/cmake/CMakeLists.dependencies.txt +++ b/buildTools/cmake/CMakeLists.dependencies.txt @@ -92,10 +92,10 @@ mark_as_advanced(ION_USE_SYSTEM_BOOST) option(ION_USE_SYSTEM_PICARD "Use picard system libraries" OFF) mark_as_advanced(ION_USE_SYSTEM_PICARD) -set(cuda_proj_version "7.5.18-19867135") -set(cuda_toolkit_tar_file "cuda-linux64-rel-7.5.18-19867135.tar.gz") -set(cuda_toolkit_patch_file "cuda_toolkit-7.5.18.patch") -set(CUDA_VERSION "7.5") +set(cuda_proj_version "8.0.44-21122537") +set(cuda_toolkit_tar_file "cuda-linux64-16.04-rel-8.0.44-21122537.tar.gz") +set(CUDA_VERSION "8.0") + set(cuda_toolkit "cuda_toolkit") set(cuda_toolkit_version "${cuda_toolkit}-${cuda_proj_version}") @@ -118,7 +118,7 @@ if(ION_USE_CUDA) CONFIGURE_COMMAND "" BUILD_COMMAND "" INSTALL_COMMAND "" - PATCH_COMMAND patch -p1 -t -N < "${PROJECT_SOURCE_DIR}/../external/${cuda_toolkit_patch_file}" + #PATCH_COMMAND patch -p1 -t -N < "${PROJECT_SOURCE_DIR}/../external/${cuda_toolkit_patch_file}" ) @@ -156,18 +156,19 @@ if(NOT ION_USE_SYSTEM_HDF5) --prefix=${PROJECT_BINARY_DIR}/../${proj_name_version}-install ) include_directories("${PROJECT_BINARY_DIR}/../${proj_name_version}-install/include") - set(ION_HDF5_LIBS "${PROJECT_BINARY_DIR}/../${proj_name_version}-install/lib/libhdf5.a") - set(ION_HDF5_HL_LIBS "${PROJECT_BINARY_DIR}/../${proj_name_version}-install/lib/libhdf5_hl.a") + set(HDF5_LIBRARIES "${PROJECT_BINARY_DIR}/../${proj_name_version}-install/lib/libhdf5.a") + set(HDF5_HL_LIBRARIES "${PROJECT_BINARY_DIR}/../${proj_name_version}-install/lib/libhdf5_hl.a") + set(ION_HDF5_LIBS "${HDF5_HL_LIBRARIES};${HDF5_LIBRARIES}") else() add_custom_target(hdf5_proj) - set(HDF5_USE_STATIC_LIBRARIES True) + set(HDF5_USE_STATIC_LIBRARIES OFF) find_package(HDF5 REQUIRED) if (${LSB_RELEASE_CODENAME} STREQUAL "trusty" OR ${LSB_RELEASE_CODENAME} STREQUAL "vivid" OR ${LSB_RELEASE_CODENAME} STREQUAL "xenial") - set(ION_HDF5_LIBS "hdf5;${HDF5_LIBRARIES}") + set(ION_HDF5_LIBS "hdf5;hdf5_hl;${HDF5_LIBRARIES}") else() - set(ION_HDF5_LIBS "${HDF5_LIBRARIES}") + set(ION_HDF5_LIBS "${HDF5_HL_LIBRARIES};${HDF5_LIBRARIES}") endif() if (${LSB_RELEASE_CODENAME} STREQUAL "vivid" OR ${LSB_RELEASE_CODENAME} STREQUAL "xenial") diff --git a/buildTools/terms-of-use.txt b/buildTools/terms-of-use.txt index c05f8f2d..6fa2cdce 100644 --- a/buildTools/terms-of-use.txt +++ b/buildTools/terms-of-use.txt @@ -1,414 +1,647 @@ +Torrent Suite Software Version 5.4 ===================================================================== -1. Torrent Suite Software License -===================================================================== - -Torrent Suite Software: This software provides for the analysis and storage of data received from the Ion Personal Genome Machine(R) PGM(tm) Sequencer or the Ion Proton(R) Sequencer. ---------------------------------------------------------------------- - -Copyright (C) 2011 Life Technologies Corporation +Torrent Suite(tm) Software v5.4: This software provides for the analysis and storage of data received from the Ion Personal Genome Machine(tm) Sequencer, the Ion Proton(tm) Sequencer, the Ion S5(tm) Sequencer or the Ion S5(tm) XL Sequencer. +April 19, 2017 +Copyright (C) 2017 Life Technologies Corporation All Rights Reserved. This program is free software; you can redistribute it and/or modify it -under the terms of the GNU General Public License as published by the -Free Software Foundation; either version 2 of the License, or (at your -option) any later version. +under the terms of the GNU General Public License version 2 as published by the +Free Software Foundation. This program is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without even the implied warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU -General Public License for more details. +General Public License version 2 for more details. -You should have received a copy of the GNU General Public License along -with this program; if not, write to the Free Software Foundation, Inc., -59 Temple Place - Suite 330, Boston, MA 02111-1307, USA. You can also find the -GPL on the GNU web site: . +You should have received a copy of the GNU General Public License version 2 along +with this program (see Section 2 below); if not, write to the Free Software Foundation, Inc., +59 Temple Place - Suite 330, Boston, MA 02111-1307, USA. You can also find the +GPL version 2 on the GNU web site: https://www.gnu.org/licenses/gpl-2.0.html + +1. Third Party Software Notices and Licenses ===================================================================== -2. Third Party Software Notices and Licenses. -===================================================================== + +This software uses third party software components from several sources. Portions of these software components are copyrighted and licensed by their respective owners as indicated below. Various licenses require distribution of source code or if a link is used to point the end-user to a source-code repository, and the source code is not available at such site, the distributor must, for a time determined by license, offer to provide source code. In such cases, please contact your Life Technologies representative. As well, various licenses require that the end-user receive a copy of the license or certain notices. Such licenses and notices may be found below. In order to use this software, the end-user must abide by the terms and conditions of these third party licenses. + +Armadillo +------------------------------------------------------------- + +Mozilla Public License Version 2.0 +------------------------------------------------------------- + +1. Definitions +------------------------------------------------------------- + +1.1. "Contributor" + means each individual or legal entity that creates, contributes to + the creation of, or owns Covered Software. +1.2. "Contributor Version" + means the combination of the Contributions of others (if any) used + by a Contributor and that particular Contributor's Contribution. + +1.3. "Contribution" + means Covered Software of a particular Contributor. + +1.4. "Covered Software" + means Source Code Form to which the initial Contributor has attached + the notice in Exhibit A, the Executable Form of such Source Code + Form, and Modifications of such Source Code Form, in each case + including portions thereof. + +1.5. "Incompatible With Secondary Licenses" + means + + (a) that the initial Contributor has attached the notice described + in Exhibit B to the Covered Software; or + + (b) that the Covered Software was made available under the terms of + version 1.1 or earlier of the License, but not also under the + terms of a Secondary License. + +1.6. "Executable Form" + means any form of the work other than Source Code Form. + +1.7. "Larger Work" + means a work that combines Covered Software with other material, in + a separate file or files, that is not Covered Software. + +1.8. "License" + means this document. + +1.9. "Licensable" + means having the right to grant, to the maximum extent possible, + whether at the time of the initial grant or subsequently, any and + all of the rights conveyed by this License. + +1.10. "Modifications" + means any of the following: + + (a) any file in Source Code Form that results from an addition to, + deletion from, or modification of the contents of Covered + Software; or + + (b) any new file in Source Code Form that contains any Covered + Software. + +1.11. "Patent Claims" of a Contributor + means any patent claim(s), including without limitation, method, + process, and apparatus claims, in any patent Licensable by such + Contributor that would be infringed, but for the grant of the + License, by the making, using, selling, offering for sale, having + made, import, or transfer of either its Contributions or its + Contributor Version. + +1.12. "Secondary License" + means either the GNU General Public License, Version 2.0, the GNU + Lesser General Public License, Version 2.1, the GNU Affero General + Public License, Version 3.0, or any later versions of those + licenses. + +1.13. "Source Code Form" + means the form of the work preferred for making modifications. + +1.14. "You" (or "Your") + means an individual or a legal entity exercising rights under this + License. For legal entities, "You" includes any entity that + controls, is controlled by, or is under common control with You. For + purposes of this definition, "control" means (a) the power, direct + or indirect, to cause the direction or management of such entity, + whether by contract or otherwise, or (b) ownership of more than + fifty percent (50%) of the outstanding shares or beneficial + ownership of such entity. + +2. License Grants and Conditions +------------------------------------------------------------- + +2.1. Grants + +Each Contributor hereby grants You a world-wide, royalty-free, +non-exclusive license: + +(a) under intellectual property rights (other than patent or trademark) + Licensable by such Contributor to use, reproduce, make available, + modify, display, perform, distribute, and otherwise exploit its + Contributions, either on an unmodified basis, with Modifications, or + as part of a Larger Work; and + +(b) under Patent Claims of such Contributor to make, use, sell, offer + for sale, have made, import, and otherwise transfer either its + Contributions or its Contributor Version. + +2.2. Effective Date + +The licenses granted in Section 2.1 with respect to any Contribution +become effective for each Contribution on the date the Contributor first +distributes such Contribution. + +2.3. Limitations on Grant Scope + +The licenses granted in this Section 2 are the only rights granted under +this License. No additional rights or licenses will be implied from the +distribution or licensing of Covered Software under this License. +Notwithstanding Section 2.1(b) above, no patent license is granted by a +Contributor: + +(a) for any code that a Contributor has removed from Covered Software; + or + +(b) for infringements caused by: (i) Your and any other third party's + modifications of Covered Software, or (ii) the combination of its + Contributions with other software (except as part of its Contributor + Version); or + +(c) under Patent Claims infringed by Covered Software in the absence of + its Contributions. + +This License does not grant any rights in the trademarks, service marks, +or logos of any Contributor (except as may be necessary to comply with +the notice requirements in Section 3.4). + +2.4. Subsequent Licenses + +No Contributor makes additional grants as a result of Your choice to +distribute the Covered Software under a subsequent version of this +License (see Section 10.2) or under the terms of a Secondary License (if +permitted under the terms of Section 3.3). + +2.5. Representation + +Each Contributor represents that the Contributor believes its +Contributions are its original creation(s) or it has sufficient rights +to grant the rights to its Contributions conveyed by this License. + +2.6. Fair Use + +This License is not intended to limit any rights You have under +applicable copyright doctrines of fair use, fair dealing, or other +equivalents. + +2.7. Conditions + +Sections 3.1, 3.2, 3.3, and 3.4 are conditions of the licenses granted +in Section 2.1. + +3. Responsibilities +------------------------------------------------------------- + +3.1. Distribution of Source Form + +All distribution of Covered Software in Source Code Form, including any +Modifications that You create or to which You contribute, must be under +the terms of this License. You must inform recipients that the Source +Code Form of the Covered Software is governed by the terms of this +License, and how they can obtain a copy of this License. You may not +attempt to alter or restrict the recipients' rights in the Source Code +Form. + +3.2. Distribution of Executable Form + +If You distribute Covered Software in Executable Form then: + +(a) such Covered Software must also be made available in Source Code + Form, as described in Section 3.1, and You must inform recipients of + the Executable Form how they can obtain a copy of such Source Code + Form by reasonable means in a timely manner, at a charge no more + than the cost of distribution to the recipient; and + +(b) You may distribute such Executable Form under the terms of this + License, or sublicense it under different terms, provided that the + license for the Executable Form does not attempt to limit or alter + the recipients' rights in the Source Code Form under this License. + +3.3. Distribution of a Larger Work + +You may create and distribute a Larger Work under terms of Your choice, +provided that You also comply with the requirements of this License for +the Covered Software. If the Larger Work is a combination of Covered +Software with a work governed by one or more Secondary Licenses, and the +Covered Software is not Incompatible With Secondary Licenses, this +License permits You to additionally distribute such Covered Software +under the terms of such Secondary License(s), so that the recipient of +the Larger Work may, at their option, further distribute the Covered +Software under the terms of either this License or such Secondary +License(s). + +3.4. Notices + +You may not remove or alter the substance of any license notices +(including copyright notices, patent notices, disclaimers of warranty, +or limitations of liability) contained within the Source Code Form of +the Covered Software, except that You may alter any license notices to +the extent required to remedy known factual inaccuracies. + +3.5. Application of Additional Terms + +You may choose to offer, and to charge a fee for, warranty, support, +indemnity or liability obligations to one or more recipients of Covered +Software. However, You may do so only on Your own behalf, and not on +behalf of any Contributor. You must make it absolutely clear that any +such warranty, support, indemnity, or liability obligation is offered by +You alone, and You hereby agree to indemnify every Contributor for any +liability incurred by such Contributor as a result of warranty, support, +indemnity or liability terms You offer. You may include additional +disclaimers of warranty and limitations of liability specific to any +jurisdiction. + +4. Inability to Comply Due to Statute or Regulation +--------------------------------------------------- + +If it is impossible for You to comply with any of the terms of this +License with respect to some or all of the Covered Software due to +statute, judicial order, or regulation then You must: (a) comply with +the terms of this License to the maximum extent possible; and (b) +describe the limitations and the code they affect. Such description must +be placed in a text file included with all distributions of the Covered +Software under this License. Except to the extent prohibited by statute +or regulation, such description must be sufficiently detailed for a +recipient of ordinary skill to be able to understand it. + +5. Termination +------------------------------------------------------------- + +5.1. The rights granted under this License will terminate automatically +if You fail to comply with any of its terms. However, if You become +compliant, then the rights granted under this License from a particular +Contributor are reinstated (a) provisionally, unless and until such +Contributor explicitly and finally terminates Your grants, and (b) on an +ongoing basis, if such Contributor fails to notify You of the +non-compliance by some reasonable means prior to 60 days after You have +come back into compliance. Moreover, Your grants from a particular +Contributor are reinstated on an ongoing basis if such Contributor +notifies You of the non-compliance by some reasonable means, this is the +first time You have received notice of non-compliance with this License +from such Contributor, and You become compliant prior to 30 days after +Your receipt of the notice. + +5.2. If You initiate litigation against any entity by asserting a patent +infringement claim (excluding declaratory judgment actions, +counter-claims, and cross-claims) alleging that a Contributor Version +directly or indirectly infringes any patent, then the rights granted to +You by any and all Contributors for the Covered Software under Section +2.1 of this License shall terminate. + +5.3. In the event of termination under Sections 5.1 or 5.2 above, all +end user license agreements (excluding distributors and resellers) which +have been validly granted by You or Your distributors under this License +prior to termination shall survive termination. + + + +6. Disclaimer of Warranty +------------------------------------------------------------- + +Covered Software is provided under this License on an "as is" basis, without +warranty of any kind, either expressed, implied, or statutory, including, +without limitation, warranties that the Covered Software is free of defects, +merchantable, fit for a particular purpose or non-infringing. The entire risk +as to the quality and performance of the Covered Software is with You. Should +any Covered Software prove defective in any respect, You (not any Contributor) +assume the cost of any necessary servicing, repair, or correction. This +disclaimer of warranty constitutes an essential part of this License. No use of +any Covered Software is authorized under this License except under this +disclaimer. + + +7. Limitation of Liability +------------------------------------------------------------- + +Under no circumstances and under no legal theory, whether tort (including +negligence), contract, or otherwise, shall any Contributor, or anyone who +distributes Covered Software as permitted above, be liable to You for any +direct, indirect, special, incidental, or consequential damages of any +character including, without limitation, damages for lost profits, loss of +goodwill, work stoppage, computer failure or malfunction, or any and all other +commercial damages or losses, even if such party shall have been informed of +the possibility of such damages. This limitation of liability shall not apply +to liability for death or personal injury resulting from such party's +negligence to the extent applicable law prohibits such limitation. Some +jurisdictions do not allow the exclusion or limitation of incidental or +consequential damages, so this exclusion and limitation may not apply to You. + + +8. Litigation +------------------------------------------------------------- -The following third party software components are included in the Torrent Suite -Software. These software components are copyrighted and licensed by their respective -owners. Various components require distribution of source code or if a URL is used to -point the end-user to a source-code repository, and the source code is not available at -such site, the distributor must, for a time determined by the license, offer to provide the -source code. In such cases, please contact your Ion Torrent representative. As well, -various licenses require that the end-user receive a copy of the license and such licenses -may be found below. In order to use this Torrent Suite Software, the end-user must -abide by the terms and conditions of these third-party licenses. +Any litigation relating to this License may be brought only in the +courts of a jurisdiction where the defendant maintains its principal +place of business and such litigation shall be governed by laws of that +jurisdiction, without reference to its conflict-of-law provisions. +Nothing in this Section shall prevent a party's ability to bring +cross-claims or counter-claims. -lapack -URL: http://www.netlib.org/lapack/ -License: BSD +9. Miscellaneous +------------------------------------------------------------- -k-means -URL: http://www.cs.umd.edu/~mount/Projects/KMeans/ -License: GPL +This License represents the complete agreement concerning the subject +matter hereof. If any provision of this License is held to be +unenforceable, such provision shall be reformed only to the extent +necessary to make it enforceable. Any law or regulation which provides +that the language of a contract shall be construed against the drafter +shall not be used to construe this License against a Contributor. -boost -URL: http://www.boost.org/ -License: BSD +10. Versions of the License +------------------------------------------------------------- -atlas -URL: http://math-atlas.sourceforge.net -License: BSD +10.1. New Versions -libgfortran -License: GPL +Mozilla Foundation is the license steward. Except as provided in Section +10.3, no one other than the license steward has the right to modify or +publish new versions of this License. Each version will be given a +distinguishing version number. -LAPACK ---------------------------------------------------------------------- -Copyright (c) 1992-2008 The University of Tennessee. All rights -reserved. +10.2. Effect of New Versions -$COPYRIGHT$ +You may distribute the Covered Software under the terms of the version +of the License under which You originally received the Covered Software, +or under the terms of any subsequent version published by the license +steward. -Additional copyrights may follow +10.3. Modified Versions -$HEADER$ +If you create software not governed by this License, and you want to +create a new license for such software, you may create and use a +modified version of this License if you rename the license and remove +any references to the name of the license steward (except to note that +such modified license differs from this License). + +10.4. Distributing Source Code Form that is Incompatible With Secondary +Licenses + +If You choose to distribute Source Code Form that is Incompatible With +Secondary Licenses under the terms of this version of the License, the +notice described in Exhibit B of this License must be attached. + +Exhibit A - Source Code Form License Notice +------------------------------------------------------------- + +This Source Code Form is subject to the terms of the Mozilla Public +License, v. 2.0. If a copy of the MPL was not distributed with this +file, You can obtain one at http://mozilla.org/MPL/2.0/. + +If it is not possible or desirable to put the notice in a particular +file, then You may include the notice in a location (such as a LICENSE +file in a relevant directory) where a recipient would be likely to look +for such a notice. + +You may add additional accurate notices of copyright ownership. + +Exhibit B - "Incompatible With Secondary Licenses" Notice +------------------------------------------------------------- + +This Source Code Form is "Incompatible With Secondary Licenses", as +defined by the Mozilla Public License, v. 2.0. + + +HTS Lib and Samtools +------------------------------------------------------------- + +The MIT/Expat License +Copyright (C) 2008-2014 Genome Research Ltd. + +Permission is hereby granted, free of charge, to any person obtaining a copy +of this software and associated documentation files (the "Software"), to deal +in the Software without restriction, including without limitation the rights +to use, copy, modify, merge, publish, distribute, sublicense, and/or sell +copies of the Software, and to permit persons to whom the Software is +furnished to do so, subject to the following conditions: +The above copyright notice and this permission notice shall be included in +all copies or substantial portions of the Software. +THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR +IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, +FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL +THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER +LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING +FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER +DEALINGS IN THE SOFTWARE. + + +VCF Lib +------------------------------------------------------------- + +The MIT License (see text above) +Copyright (c) 2012 Erik Garrison + +Blas and Lapack +------------------------------------------------------------- +Copyright (c) 1992-2013 The University of Tennessee and The University of Tennessee Research Foundation. All rights reserved. + +Copyright (c) 2000-2013 The University of California Berkeley. All rights reserved. + +Copyright (c) 2006-2013 The University of Colorado Denver. All rights reserved. Redistribution and use in source and binary forms, with or without modification, are permitted provided that the following conditions are met: -- Redistributions of source code must retain the above copyright - notice, this list of conditions and the following disclaimer. - -- Redistributions in binary form must reproduce the above copyright - notice, this list of conditions and the following disclaimer listed - in this license in the documentation and/or other materials - provided with the distribution. - -- Neither the name of the copyright holders nor the names of its - contributors may be used to endorse or promote products derived from - this software without specific prior written permission. - + - Redistributions of source code must retain the above copyright + notice, this list of conditions and the following disclaimer. + - Redistributions in binary form must reproduce the above copyright + notice, this list of conditions and the following disclaimer listed + in this license in the documentation and/or other materials + provided with the distribution. + - Neither the name of the copyright holders nor the names of its + contributors may be used to endorse or promote products derived from + this software without specific prior written permission. + +The copyright holders provide no reassurances that the source code +provided does not infringe any patent, copyright, or any other +intellectual property rights of third parties. The copyright holders +disclaim any liability to any recipient for claims brought against +recipient by any third party for infringement of that parties +intellectual property rights. + THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS -"AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT +"AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR -A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE COPYRIGHT +A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE COPYRIGHT OWNER OR CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY -THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT +THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE -OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH -DAMAGE. +OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE. +Django +------------------------------------------------------------- +Copyright (c) Django Software Foundation and individual contributors. All rights reserved. -K-MEANS ---------------------------------------------------------------------- -KMlocal: A testbed for k-means clustering algorithms based on local -search -Version: 1.7 -Date: 08/10/2005 +Redistribution and use in source and binary forms, with or without modification, +are permitted provided that the following conditions are met: -Copyright (C) 2004-2005 David M. Mount and University of Maryland -All Rights Reserved. +1. Redistributions of source code must retain the above copyright notice, + this list of conditions and the following disclaimer. +2. Redistributions in binary form must reproduce the above copyright + notice, this list of conditions and the following disclaimer in the + documentation and/or other materials provided with the distribution. +3. Neither the name of Django nor the names of its contributors may be used + to endorse or promote products derived from this software without + specific prior written permission. -This program is free software; you can redistribute it and/or modify it -under the terms of the GNU General Public License as published by the -Free Software Foundation; either version 2 of the License, or (at your -option) any later version. +THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS "AS IS" AND +ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED +WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE +DISCLAIMED. IN NO EVENT SHALL THE COPYRIGHT OWNER OR CONTRIBUTORS BE LIABLE FOR +ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES +(INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; +LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON +ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT +(INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THIS +SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE. -This program is distributed in the hope that it will be useful, but -WITHOUT ANY WARRANTY; without even the implied warranty of -MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU -General Public License for more details. - -You should have received a copy of the GNU General Public License along -with this program; if not, write to the Free Software Foundation, Inc., -59 Temple Place - Suite 330, Boston, MA 02111-1307, USA. - -Disclaimer ----------- -The University of Maryland and the authors make no representations -about the suitability or fitness of this software for any purpose. -It is provided "as is" without express or implied warranty. - -Author ------- -David Mount -Dept of Computer Science -University of Maryland, -College Park, MD 20742 USA -mount@cs.umd.edu -http://www.cs.umd.edu/~mount/ - - - -BOOST ---------------------------------------------------------------------- -Boost Software License - Version 1.0 - August 17th, 2003 - -Permission is hereby granted, free of charge, to any person or -organization obtaining a copy of the software and accompanying -documentation covered by this license (the "Software") to use, -reproduce, display, distribute, execute, and transmit the Software, and -to prepare derivative works of the Software, and to permit third-parties -to whom the Software is furnished to do so, all subject to the -following: - -The copyright notices in the Software and this entire statement, -including the above license grant, this restriction and the following -disclaimer, must be included in all copies of the Software, in whole or -in part, and all derivative works of the Software, unless such copies or -derivative works are solely in the form of machine-executable object -code generated by a source language processor. - -THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS -OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF -MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE, TITLE AND NON- -INFRINGEMENT. IN NO EVENT SHALL THE COPYRIGHT HOLDERS OR ANYONE -DISTRIBUTING THE SOFTWARE BE LIABLE FOR ANY DAMAGES OR OTHER LIABILITY, -WHETHER IN CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN -CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE -SOFTWARE. - - -ATLAS ---------------------------------------------------------------------- -Upstream Authors: -R. Clint Whaley atlas@cs.utk.edu -Jack Dongarra atlas@cs.utk.edu - -Copyright: -(C) Copyright 1997-2008 All Rights Reserved - -Permission to use, copy, modify, and distribute this software and -its documentation for any purpose and without fee is hereby granted -provided that the above copyright notice appear in all copies and -that both the copyright notice and this permission notice appear in -supporting documentation. - -Neither the University of Tennessee nor the Author make any -representations about the suitability of this software for any -purpose. This software is provided ``as is'' without express or -implied warranty. - -On alpha architectures, the standard builds use K. GOTO's kernels -contributed to the ATLAS project under the LGPL. Therefore on these -platforms, the distributed prebuilt libraries, as well as any user -built library using this kernel, is licensed under the LGPL. - -On Debian GNU/Linux systems, the complete text of the GNU Lesser General -Public License can be found in `/usr/share/common-licenses/LGPL'. +Cuda Toolkit +------------------------------------------------------------- +END USER LICENSE AGREEMENT (EULA) -===================================================================== -3. 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This Agreement will automatically terminate if Licensee fails to comply with any of the terms and conditions hereof. In such event, Licensee must destroy all copies of the SOFTWARE and all of its component parts. Defensive Suspension If Licensee commences or participates in any legal proceeding against NVIDIA, then NVIDIA may, in its sole discretion, suspend or terminate all license grants and any other rights provided under this Agreement during the pendency of such legal proceedings. + +1.4. Copyright All rights, title, interest and copyrights in and to the SOFTWARE (including but not limited to all images, photographs, animations, video, audio, music, text, and other information incorporated into the SOFTWARE), the accompanying printed materials, and any copies of the SOFTWARE, are owned by NVIDIA, or its suppliers. The SOFTWARE is protected by copyright laws and international treaty provisions. Accordingly, Licensee is required to treat the SOFTWARE like any other copyrighted material, except as otherwise allowed pursuant to this Agreement and that it may make one copy of the SOFTWARE solely for backup or archive purposes. RESTRICTED RIGHTS NOTICE. Software has been developed entirely at private expense and is commercial computer software provided with RESTRICTED RIGHTS. Use, duplication or disclosure by the U.S. Government or a U.S. Government subcontractor is subject to the restrictions set forth in the Agreement under which Software was obtained pursuant to DFARS 227.7202-3(a) or as set forth in subparagraphs (c)(1) and (2) of the Commercial Computer Software - Restricted Rights clause at FAR 52.227-19, as applicable. Contractor/manufacturer is NVIDIA, 2701 San Tomas Expressway, Santa Clara, CA 95050. + +1.5. Applicable Law This Agreement shall be deemed to have been made in, and shall be construed pursuant to, the laws of the State of Delaware. The United Nations Convention on Contracts for the International Sale of Goods is specifically disclaimed. The courts of Santa Clara County, California shall have exclusive jurisdiction and venue over any dispute arising out of or relating to this Agreement. + +1.6. Disclaimer of Warranties and Limitations on Liability + + 1.6.1. No Warranties TO THE MAXIMUM EXTENT PERMITTED BY APPLICABLE LAW, THE SOFTWARE IS PROVIDED "AS IS" AND NVIDIA AND ITS SUPPLIERS DISCLAIM ALL WARRANTIES, EITHER EXPRESS OR IMPLIED, INCLUDING, BUT NOT LIMITED TO, IMPLIED WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE, OR NONINFRINGEMENT. + + 1.6.2. No Liability for Consequential Damages TO THE MAXIMUM EXTENT PERMITTED BY APPLICABLE LAW, IN NO EVENT SHALL NVIDIA OR ITS SUPPLIERS BE LIABLE FOR ANY SPECIAL, INCIDENTAL, INDIRECT, OR CONSEQUENTIAL DAMAGES WHATSOEVER (INCLUDING, WITHOUT LIMITATION, DAMAGES FOR LOSS OF BUSINESS PROFITS, BUSINESS INTERRUPTION, LOSS OF BUSINESS INFORMATION, OR ANY OTHER PECUNIARY LOSS) ARISING OUT OF THE USE OF OR INABILITY TO USE THE SOFTWARE, EVEN IF NVIDIA HAS BEEN ADVISED OF THE POSSIBILITY OF SUCH DAMAGES. + + 1.6.3. No Support . NVIDIA has no obligation to support or to provide any updates of the Software. + +1.7. Miscellaneous + + 1.7.1. Feedback Notwithstanding any Non-Disclosure Agreement executed by and between the parties, the parties agree that in the event Licensee or NVIDIA provides Feedback (as defined below) to the other party on how to design, implement, or improve the SOFTWARE or Licensee's product(s) for use with the SOFTWARE, the following terms and conditions apply the Feedback: + + 1.7.1.1. Exchange of Feedback Both parties agree that neither party has an obligation to give the other +party any suggestions, comments or other feedback, whether verbally or in written or source code form, relating to (i) the SOFTWARE; (ii) Licensee's products; (iii) Licensee's use of the SOFTWARE; or (iv) optimization/interoperability of Licensee's product with the SOFTWARE (collectively defined as "Feedback"). In the event either party provides Feedback to the other party, the party receiving the Feedback may use any Feedback that the other party voluntarily provides to improve the (i) SOFTWARE or other related NVIDIA technologies, respectively for the benefit of NVIDIA; or (ii) Licensee's product or other related Licensee technologies, respectively for the benefit of Licensee. Accordingly, if either party provides Feedback to the other party, both parties agree that the other party and its respective licensees may freely use, reproduce, license, distribute, and otherwise commercialize the Feedback in the (i) SOFTWARE or other related technologies; or (ii) Licensee's products or other related technologies, respectively, without the payment of any royalties or fees. + + 1.7.1.2. Residual Rights Licensee agrees that NVIDIA shall be free to use any general knowledge, skills and experience, (including, but not limited to, ideas, concepts, know-how, or techniques) ("Residuals"), contained in the (i) Feedback provided by Licensee to NVIDIA; (ii) Licensee's products shared or disclosed to NVIDIA in connection with the Feedback; or (c) Licensee's confidential information voluntarily provided to NVIDIA in connection with the Feedback, which are retained in the memories of NVIDIA's employees, agents, or contractors who have had access to such Residuals. Subject to the terms and conditions of this Agreement, NVIDIA's employees, agents, or contractors shall not be prevented from using Residuals as part of such employee's, agent's or contractor's general knowledge, skills, experience, talent, and/or expertise. NVIDIA shall not have any obligation to limit or restrict the assignment of such employees, agents or contractors or to pay royalties for any work resulting from the use of Residuals. + + 1.7.1.3. Disclaimer of Warranty FEEDBACK FROM EITHER PARTY IS PROVIDED FOR THE OTHER PARTY'S USE "AS IS" AND BOTH PARTIES DISCLAIM ALL WARRANTIES, EXPRESS, IMPLIED AND STATUTORY INCLUDING, WITHOUT LIMITATION, THE IMPLIED WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE, OR NONINFRINGEMENT. BOTH PARTIES DO NOT REPRESENT OR WARRANT THAT THE FEEDBACK WILL MEET THE OTHER PARTY'S REQUIREMENTS OR THAT THE OPERATION OR IMPLEMENTATION OF THE FEEDBACK WILL BE UNINTERRUPTED OR ERROR-FREE. + 1.7.1.4. No Liability for Consequential Damages TO THE MAXIMUM EXTENT PERMITTED BY APPLICABLE LAW, IN NO EVENT SHALL EITHER PARTY OR ITS SUPPLIERS BE LIABLE FOR ANY SPECIAL, INCIDENTAL, INDIRECT, OR CONSEQUENTIAL DAMAGES WHATSOEVER (INCLUDING, WITHOUT LIMITATION, DAMAGES FOR LOSS OF BUSINESS PROFITS, BUSINESS INTERRUPTION, LOSS OF BUSINESS INFORMATION, OR ANY OTHER PECUNIARY LOSS) ARISING OUT OF THE USE OF OR INABILITY TO USE THE FEEDBACK, EVEN IF THE OTHER PARTY HAS BEEN ADVISED OF THE POSSIBILITY OF SUCH DAMAGES. + + 1.7.2. Freedom of Action Licensee agrees that this Agreement is nonexclusive and NVIDIA may currently or in the future be developing software, other technology or confidential information internally, or receiving confidential information from other parties that maybe similar to the Feedback and Licensee's confidential information (as provided in Section 1.7.1.2 above), which may be provided to NVIDIA in connection with Feedback by Licensee. Accordingly, Licensee agrees that nothing in this Agreement will be construed as a representation or inference that NVIDIA will not develop, design, manufacture, acquire, market products, or have products developed, designed, manufactured, acquired, or marketed for NVIDIA, that compete with the Licensee's products or confidential information. + + 1.7.3. No Implied Licenses Under no circumstances should anything in this Agreement be construed as NVIDIA granting by implication, estoppel or otherwise, (i) a license to any NVIDIA product or technology other than the SOFTWARE; or (ii) any additional license rights for the SOFTWARE other than the licenses expressly granted in this Agreement. If any provision of this Agreement is inconsistent with, or cannot be fully enforced under, the law, such provision will be construed as limited to the extent necessary to be consistent with and fully enforceable under the law. This Agreement is the final, complete and exclusive agreement between the parties relating to the subject matter hereof, and supersedes all prior or contemporaneous understandings and agreements relating to such subject matter, whether oral or written. This Agreement may only be modified in writing signed by an authorized officer of NVIDIA. Licensee agrees that it will not ship, transfer or export the SOFTWARE into any country, or use the SOFTWARE in any manner, prohibited by the United States Bureau of Industry and Security or any export laws, restrictions or regulations. The parties agree that the following sections of the Agreement will survive the termination of the License: Section 1.2.1.4, Section 1.4, Section 1.5, Section 1.6, and Section 1.7. + +1.8. Attachment A Redistributable Software In connection with Section 1.2.1.1 of this Agreement, the following files may be redistributed with software applications developed by Licensee, including certain variations of these files that have version number or architecture specific information NVIDIA CUDA Toolkit License Agreement www.nvidia.com End User License Agreements (EULA) DR-06739-001_v01_v8.0 | 9 embedded in the file name - as an example only, for release version 6.0 of the 64-bit Windows software, the file cudart64_60.dll is redistributable. +Component : CUDA Runtime Windows : cudart.dll, cudart_static.lib, cudadevrt.lib Mac OSX : libcudart.dylib, libcudart_static.a, libcudadevrt.a Linux : libcudart.so, libcudart_static.a, libcudadevrt.a Android : libcudart.so, libcudart_static.a, libcudadevrt.a Component : CUDA FFT Library Windows : cufft.dll, cufftw.dll Mac OSX : libcufft.dylib, libcufft_static.a, libcufftw.dylib, libcufftw_static.a Linux : libcufft.so, libcufft_static.a, libcufftw.so, libcufftw_static.a Android : libcufft.so, libcufft_static.a, libcufftw.so, libcufftw_static.a Component : CUDA BLAS Library Windows : cublas.dll, cublas_device.lib Mac OSX : libcublas.dylib, libcublas_static.a, libcublas_device.a Linux : libcublas.so, libcublas_static.a, libcublas_device.a Android : libcublas.so, libcublas_static.a, libcublas_device.a Component : NVIDIA "Drop-in" BLAS Library Windows : nvblas.dll Mac OSX : libnvblas.dylib Linux : libnvblas.so Component : CUDA Sparse Matrix Library Windows : cusparse.dll Mac OSX : libcusparse.dylib, libcusparse_static.a Linux : libcusparse.so, libcusparse_static.a Android : libcusparse.so, libcusparse_static.a Component : CUDA Linear Solver Library Windows : cusolver.dll Mac OSX : libcusolver.dylib, libcusolver_static.a Linux : libcusolver.so, libcusolver_static.a Android : libcusolver.so, libcusolver_static.a Component : CUDA Random Number Generation Library Windows : curand.dll Mac OSX : libcurand.dylib, libcurand_static.a Linux : libcurand.so, libcurand_static.a Android : libcurand.so, libcurand_static.a Component : NVIDIA Performance Primitives Library Windows : nppc.dll, nppi.dll, npps.dll Mac OSX : libnppc.dylib, libnppi.dylib, libnpps.dylib, libnppc_static.a, libnpps_static.a, libnppi_static.a Linux : libnppc.so, libnppi.so, libnpps.so, libnppc_static.a, libnpps_static.a, libnppi_static.a Android : libnppc.so, libnppi.so, libnpps.so, libnppc_static.a, libnpps_static.a, libnppi_static.a Component : Internal common library required for statically linking to cuBLAS, cuSPARSE, cuFFT, cuRAND and NPP Mac OSX : libculibos.a Linux : libculibos.a Component : NVIDIA Runtime Compilation Library Windows : nvrtc.dll, nvrtc-builtins.dll Mac OSX : libnvrtc.dylib, libnvrtc-builtins.dylib Linux : libnvrtc.so, libnvrtc-builtins.so Component : NVIDIA Optimizing Compiler Library Windows : nvvm.dll Mac OSX : libnvvm.dylib Linux : libnvvm.so Component : NVIDIA Common Device Math Functions Library Windows : libdevice.compute_20.bc, libdevice.compute_30.bc, libdevice.compute_35.bc Mac OSX : libdevice.compute_20.bc, libdevice.compute_30.bc, libdevice.compute_35.bc Linux : libdevice.compute_20.bc, libdevice.compute_30.bc, libdevice.compute_35.bc Component : CUDA Occupancy Calculation Header Library All : cuda_occupancy.h Component : Profiling Tools Interface Library Windows : cupti.dll Mac OSX : libcupti.dylib Linux : libcupti.so + +1.9. Attachment B Additional Licensing Obligations The following third party components included in the SOFTWARE are licensed to Licensee pursuant to the following terms and conditions: + + +1. Licensee's use of the GDB third party component is subject to the terms and conditions of GNU GPL v3: This product includes copyrighted third-party software licensed under the terms of the GNU General Public License v3 ("GPL v3"). All third-party software packages are copyright by their respective authors. GPL v3 terms and conditions are hereby incorporated into the Agreement by this reference: http://www.gnu.org/licenses/gpl.txt Consistent with these licensing requirements, the software listed below is provided under the terms of the specified open source software licenses. To obtain source code for software provided under licenses that require redistribution of source code, including the GNU General Public License (GPL) and GNU Lesser General Public License (LGPL), contact oss-requests@nvidia.com. This offer is valid for a period of three (3) years from the date of the distribution of this product by NVIDIA CORPORATION. Component License CUDA-GDB GPL v3 + +2. Licensee represents and warrants that any and all third party licensing and/or royalty payment obligations in connection with Licensee's use of the H.264 video codecs are solely the responsibility of Licensee. + +3. Licensee's use of the Thrust library is subject to the terms and conditions of the Apache License Version 2.0. All third-party software packages are copyright by their respective authors. Apache License Version 2.0 terms and conditions are hereby incorporated into the Agreement by this reference. http://www.apache.org/licenses/ LICENSE-2.0.html. In addition, Licensee acknowledges the following notice: Thrust includes source code from the Boost Iterator, Tuple, System, and Random Number libraries. Boost Software License - Version 1.0 - August 17th, 2003 . . . . Permission is hereby granted, free of charge, to any person or organization obtaining a copy of the software and accompanying documentation covered by this license (the "Software") to use, reproduce, display, distribute, execute, and transmit the Software, and to prepare derivative works of the Software, and to permit third-parties to whom the Software is furnished to do so, all subject to the following: The copyright notices in the Software and this entire statement, including the above license grant, this restriction and the following disclaimer, must be included in all copies of the Software, in whole or in part, and all derivative works of the Software, unless such copies or derivative works are solely in the form of machine-executable object code generated by a source language processor. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE, TITLE AND NON-INFRINGEMENT. IN NO EVENT SHALL THE COPYRIGHT HOLDERS OR ANYONE DISTRIBUTING THE SOFTWARE BE LIABLE FOR ANY DAMAGES OR OTHER LIABILITY, WHETHER IN CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE. + +4. Licensee's use of the LLVM third party component is subject to the following terms and conditions: + + LLVM Release License + + University of Illinois/NCSA Open Source License Copyright (c) 2003-2010 University of Illinois at Urbana-Champaign. All rights reserved. Developed by: LLVM Team University of Illinois at Urbana-Champaign http://llvm.org Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal with the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: * Redistributions of source code must retain the above copyright notice, this list of conditions and the following disclaimers. * Redistributions in binary form must reproduce the above copyright notice, this list of conditions and the following disclaimers in the documentation and/or other materials provided with the distribution. * Neither the names of the LLVM Team, University of Illinois at Urbana- Champaign, nor the names of its contributors may be used to endorse or promote products derived from this Software without specific prior written permission. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE CONTRIBUTORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS WITH THE SOFTWARE. + +5. Licensee's use of the PCRE third party component is subject to the following terms and conditions: + + PCRE LICENCE + + PCRE is a library of functions to support regular expressions whose syntax and semantics are as close as possible to those of the Perl 5 language. Release 8 of PCRE is distributed under the terms of the "BSD" licence, as specified below. The documentation for PCRE, supplied in the "doc" directory, is distributed under the same terms as the software itself. The basic library functions are written in C and are freestanding. Also included in the distribution is a set of C++ wrapper functions, and a justin-time compiler that can be used to optimize pattern matching. These are both optional features that can be omitted when the library is built. + + + THE BASIC LIBRARY FUNCTIONS + + Written by: Philip Hazel + Email local part: ph10 + Email domain: cam.ac.uk University of Cambridge Computing Service, Cambridge, England. + Copyright (c) 1997-2012 University of Cambridge All rights reserved. + + PCRE JUST-IN-TIME COMPILATION SUPPORT + + Written by: Zoltan Herczeg + Email local part: hzmester + Emain domain: freemail.hu + Copyright(c) 2010-2012 Zoltan Herczeg All rights reserved. + + STACK-LESS JUST-IN-TIME COMPILER + + Written by: Zoltan Herczeg Email local part: hzmester Emain domain: freemail.hu Copyright(c) 2009-2012 Zoltan Herczeg All rights reserved. + + THE C++ WRAPPER FUNCTIONS + + Contributed by: Google Inc. Copyright (c) 2007-2012, Google Inc. All rights reserved. + + THE "BSD" LICENCE + + Redistribution and use in source and binary forms, with or without modification, are permitted provided that the following conditions are met: * Redistributions of source code must retain the above copyright notice, this list of conditions and the following disclaimer. * Redistributions in binary form must reproduce the above copyright notice, this list of conditions and the following disclaimer in the documentation and/or other materials provided with the distribution. * Neither the name of the University of Cambridge nor the name of Google Inc. nor the names of their contributors may be used to endorse or promote products derived from this software without specific prior written permission. THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS "AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE COPYRIGHT OWNER OR CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE. + + +6. Some of the cuBLAS library routines were written by or derived from code written by Vasily Volkov and are subject to the Modified Berkeley Software Distribution License as follows: Copyright (c) 2007-2009, Regents of the University of California All rights reserved. Redistribution and use in source and binary forms, with or without modification, are permitted provided that the following conditions are met: * Redistributions of source code must retain the above copyright notice, this list of conditions and the following disclaimer. * Redistributions in binary form must reproduce the above copyright notice, this list of conditions and the following disclaimer in the documentation and/or other materials provided with the distribution. * Neither the name of the University of California, Berkeley nor the names of its contributors may be used to endorse or promote products derived from this software without specific prior written permission. THIS SOFTWARE IS PROVIDED BY THE AUTHOR "AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE AUTHOR BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE. + +7. Some of the cuBLAS library routines were written by or derived from code written by Davide Barbieri and are subject to the Modified Berkeley Software Distribution License as follows: Copyright (c) 2008-2009 Davide Barbieri @ University of Rome Tor Vergata. All rights reserved. Redistribution and use in source and binary forms, with or without modification, are permitted provided that the following conditions are met: * Redistributions of source code must retain the above copyright notice, this list of conditions and the following disclaimer. * Redistributions in binary form must reproduce the above copyright notice, this list of conditions and the following disclaimer in the documentation and/or other materials provided with the distribution. * The name of the author may not be used to endorse or promote products derived from this software without specific prior written permission. THIS SOFTWARE IS PROVIDED BY THE AUTHOR "AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE AUTHOR BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE. + +8. Some of the cuBLAS library routines were derived from code developed by the University of Tennessee and are subject to the Modified Berkeley Software Distribution License as follows: Copyright (c) 2010 The University of Tennessee. All rights reserved. Redistribution and use in source and binary forms, with or without modification, are permitted provided that the following conditions are met: * Redistributions of source code must retain the above copyright notice, this list of conditions and the following disclaimer. * Redistributions in binary form must reproduce the above copyright notice, this list of conditions and the following disclaimer listed in this license in the documentation and/or other materials provided with the distribution. * Neither the name of the copyright holders nor the names of its contributors may be used to endorse or promote products derived from this software without specific prior written permission. THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS "AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE COPYRIGHT OWNER OR CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE. + +9. Some of the cuBLAS library routines were written by or derived from code written by Jonathan Hogg and are subject to the Modified Berkeley Software Distribution License as follows: Copyright (c) 2012, The Science and Technology Facilities Council (STFC). All rights reserved. Redistribution and use in source and binary forms, with or without modification, are permitted provided that the following conditions are met: * Redistributions of source code must retain the above copyright notice, this list of conditions and the following disclaimer. * Redistributions in binary form must reproduce the above copyright notice, this list of conditions and the following disclaimer in the documentation and/or other materials provided with the distribution. * Neither the name of the STFC nor the names of its contributors may be used to endorse or promote products derived from this software without specific prior written permission. THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS "AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE STFC BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE. + +10. Some of the cuBLAS library routines were written by or derived from code written by Ahmad M. Abdelfattah, David Keyes, and Hatem Ltaief, and are subject to the Apache License, Version 2.0, as follows: -- (C) Copyright 2013 King Abdullah University of Science and Technology Authors: Ahmad Abdelfattah (ahmad.ahmad@kaust.edu.sa) David Keyes (david.keyes@kaust.edu.sa) Hatem Ltaief (hatem.ltaief@kaust.edu.sa) Redistribution and use in source and binary forms, with or without modification, are permitted provided that the following conditions are met: * Redistributions of source code must retain the above copyright notice, this list of conditions and the following disclaimer. * Redistributions in binary form must reproduce the above copyright notice, this list of conditions and the following disclaimer in the documentation and/or other materials provided with the distribution. * Neither the name of the King Abdullah University of Science and Technology nor the names of its contributors may be used to endorse or promote products derived from this software without specific prior written permission. THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS ``AS IS'' AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE COPYRIGHT HOLDERS OR CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE. + +11. Some of the cuSPARSE library routines were written by or derived from code written by Li-Wen Chang and are subject to the NCSA Open Source License as follows: Copyright (c) 2012, University of Illinois. All rights reserved. Developed by: IMPACT Group, University of Illinois, http:// impact.crhc.illinois.edu Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal with the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: * Redistributions of source code must retain the above copyright notice, this list of conditions and the following disclaimer. * Redistributions in binary form must reproduce the above copyright notice, this list of conditions and the following disclaimers in the documentation and/or other materials provided with the distribution. * Neither the names of IMPACT Group, University of Illinois, nor the names of its contributors may be used to endorse or promote products derived from this Software without specific prior written permission. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE CONTRIBUTORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS WITH THE SOFTWARE. + +12. Some of the cuRAND library routines were written by or derived from code written by Mutsuo Saito and Makoto Matsumoto and are subject to the following license: Copyright (c) 2009, 2010 Mutsuo Saito, Makoto Matsumoto and Hiroshima University. All rights reserved. Copyright (c) 2011 Mutsuo Saito, Makoto Matsumoto, Hiroshima University and University of Tokyo. All rights reserved. Redistribution and use in source and binary forms, with or without modification, are permitted provided that the following conditions are met: * Redistributions of source code must retain the above copyright notice, this list of conditions and the following disclaimer. * Redistributions in binary form must reproduce the above copyright notice, this list of conditions and the following disclaimer in the documentation and/or other materials provided with the distribution. * Neither the name of the Hiroshima University nor the names of its contributors may be used to endorse or promote products derived from this software without specific prior written permission. THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS "AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE COPYRIGHT OWNER OR CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE. + +13. Some of the cuRAND library routines were derived from code developed by D. E. Shaw Research and are subject to the following license: Copyright 2010-2011, D. E. Shaw Research. All rights reserved. Redistribution and use in source and binary forms, with or without modification, are permitted provided that the following conditions are met: * Redistributions of source code must retain the above copyright notice, this list of conditions, and the following disclaimer. * Redistributions in binary form must reproduce the above copyright notice, this list of conditions, and the following disclaimer in the documentation and/or other materials provided with the distribution. * Neither the name of D. E. Shaw Research nor the names of its contributors may be used to endorse or promote products derived from this software without specific prior written permission. THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS "AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE DISCLAIMED. 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Permission is hereby granted, free of charge, to any person or organization obtaining a copy of the software and accompanying documentation covered by this license (the "Software") to use, reproduce, display, distribute, execute, and transmit the Software, and to prepare derivative works of the Software, and to permit third-parties to whom the Software is furnished to do so, all subject to the following: The copyright notices in the Software and this entire statement, including the above license grant, this restriction and the following disclaimer, must be included in all copies of the Software, in whole or in part, and all derivative works of the Software, unless such copies or derivative works are solely in the form of machine-executable object code generated by a source language processor. 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IN NO EVENT SHALL THE COPYRIGHT HOLDERS OR ANYONE DISTRIBUTING THE SOFTWARE BE LIABLE FOR ANY DAMAGES OR OTHER LIABILITY, WHETHER IN CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE. + +Ansible +------------------------------------------------------------- GNU GENERAL PUBLIC LICENSE + Version 3, 29 June 2007 + Copyright (c) 2007 Free Software Foundation, Inc. + Everyone is permitted to copy and distribute verbatim copies of this license document, but changing it is not allowed. + Preamble + The GNU General Public License is a free, copyleft license for software and other kinds of works. + The licenses for most software and other practical works are designed to take away your freedom to share and change the works. By contrast, the GNU General Public License is intended to guarantee your freedom to share and change all versions of a program--to @@ -420,6 +653,7 @@ Public Licenses are designed to make sure that you have the freedom to distribut of free software (and charge for them if you wish), that you receive source code or can get it if you want it, that you can change the software or use pieces of it in new free programs, and that you know you can do these things. + To protect your rights, we need to prevent others from denying you these rights or asking you to surrender the rights. Therefore, you have certain responsibilities if you distribute copies of the software, or if you modify it: responsibilities to respect the freedom of @@ -428,13 +662,16 @@ For example, if you distribute copies of such a program, whether gratis or for a must pass on to the recipients the same freedoms that you received. You must make sure that they, too, receive or can get the source code. And you must show them these terms so they know their rights. -Developers that use the GNU GPL protect your rights with two steps: (1) assert copyright -on the software, and (2) offer you this License giving you legal permission to copy, -distribute and/or modify it. + +Developers that use the GNU GPL protect your rights with two steps: + + (1) assert copyright on the software, and + (2) offer you this License giving you legal permission to copy, distribute and/or modify it. For the developers' and authors' protection, the GPL clearly explains that there is no warranty for this free software. For both users' and authors' sake, the GPL requires that modified versions be marked as changed, so that their problems will not be attributed erroneously to authors of previous versions. + Some devices are designed to deny users access to install or run modified versions of the software inside them, although the manufacturer can do so. This is fundamentally incompatible with the aim of protecting users' freedom to change the software. The @@ -443,13 +680,16 @@ which is precisely where it is most unacceptable. Therefore, we have designed th of the GPL to prohibit the practice for those products. If such problems arise substantially in other domains, we stand ready to extend this provision to those domains in future versions of the GPL, as needed to protect the freedom of users. + Finally, every program is threatened constantly by software patents. States should not allow patents to restrict development and use of software on general-purpose computers, but in those that do, we wish to avoid the special danger that patents applied to a free program could make it effectively proprietary. To prevent this, the GPL assures that patents cannot be used to render the program non-free. The precise terms and conditions for copying, distribution and modification follow. + TERMS AND CONDITIONS + 0. Definitions. "This License" refers to version 3 of the GNU General Public License. "Copyright" also means copyright-like laws that apply to other kinds of works, such as @@ -476,6 +716,7 @@ copyright notice, and (2) tells the user that there is no warranty for the work the extent that warranties are provided), that licensees may convey the work under this License, and how to view a copy of this License. If the interface presents a list of user commands or options, such as a menu, a prominent item in the list meets this criterion. + 1. Source Code. The "source code" for a work means the preferred form of the work for making modifications to it. "Object code" means any non-source form of a work. @@ -504,6 +745,7 @@ parts of the work. The Corresponding Source need not include anything that users can regenerate automatically from other parts of the Corresponding Source. The Corresponding Source for a work in source code form is that same work. + 2. Basic Permissions. All rights granted under this License are granted for the term of copyright on the Program, and are irrevocable provided the stated conditions are met. This License @@ -521,6 +763,7 @@ exclusively on your behalf, under your direction and control, on terms that proh from making any copies of your copyrighted material outside their relationship with you. Conveying under any other circumstances is permitted solely under the conditions stated below. Sublicensing is not allowed; section 10 makes it unnecessary. + 3. Protecting Users' Legal Rights From Anti-Circumvention Law. No covered work shall be deemed part of an effective technological measure under any applicable law fulfilling obligations under article 11 of the WIPO copyright treaty @@ -531,6 +774,7 @@ technological measures to the extent such circumvention is effected by exercisin under this License with respect to the covered work, and you disclaim any intention to limit operation or modification of the work as a means of enforcing, against the work's users, your or third parties' legal rights to forbid circumvention of technological measures. + 4. Conveying Verbatim Copies. You may convey verbatim copies of the Program's source code as you receive it, in any medium, provided that you conspicuously and appropriately publish on each copy an @@ -540,22 +784,23 @@ of the absence of any warranty; and give all recipients a copy of this License a the Program. You may charge any price or no price for each copy that you convey, and you may offer support or warranty protection for a fee. + 5. Conveying Modified Source Versions. You may convey a work based on the Program, or the modifications to produce it from the Program, in the form of source code under the terms of section 4, provided that you also meet all of these conditions: -* a) The work must carry prominent notices stating that you modified it, and giving + a) The work must carry prominent notices stating that you modified it, and giving a relevant date. -* b) The work must carry prominent notices stating that it is released under this + b) The work must carry prominent notices stating that it is released under this License and any conditions added under section 7. This requirement modifies the requirement in section 4 to "keep intact all notices". -* c) You must license the entire work, as a whole, under this License to anyone who + c) You must license the entire work, as a whole, under this License to anyone who comes into possession of a copy. This License will therefore apply, along with any applicable section 7 additional terms, to the whole of the work, and all its parts, regardless of how they are packaged. This License gives no permission to license the work in any other way, but it does not invalidate such permission if you have separately received it. -* d) If the work has interactive user interfaces, each must display Appropriate Legal + d) If the work has interactive user interfaces, each must display Appropriate Legal Notices; however, if the Program has interactive interfaces that do not display Appropriate Legal Notices, your work need not make them do so. A compilation of a covered work with other separate and independent works, which are @@ -565,14 +810,15 @@ called an "aggregate" if the compilation and its resulting copyright are not use the access or legal rights of the compilation's users beyond what the individual works permit. 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You may convey a covered work in object code form under the terms of sections 4 and 5, provided that you also convey the machine-readable Corresponding Source under the terms of this License, in one of these ways: -* a) Convey the object code in, or embodied in, a physical product (including a + a) Convey the object code in, or embodied in, a physical product (including a physical distribution medium), accompanied by the Corresponding Source fixed on a durable physical medium customarily used for software interchange. -* b) Convey the object code in, or embodied in, a physical product (including a + b) Convey the object code in, or embodied in, a physical product (including a physical distribution medium), accompanied by a written offer, valid for at least three years and valid for as long as you offer spare parts or customer support for that product model, to give anyone who possesses the object code either (1) a @@ -581,11 +827,11 @@ covered by this License, on a durable physical medium customarily used for software interchange, for a price no more than your reasonable cost of physically performing this conveying of source, or (2) access to copy the Corresponding Source from a network server at no charge. -* c) Convey individual copies of the object code with a copy of the written offer to + c) Convey individual copies of the object code with a copy of the written offer to provide the Corresponding Source. This alternative is allowed only occasionally and noncommercially, and only if you received the object code with such an offer, in accord with subsection 6b. -* d) Convey the object code by offering access from a designated place (gratis or + d) Convey the object code by offering access from a designated place (gratis or for a charge), and offer equivalent access to the Corresponding Source in the same way through the same place at no further charge. You need not require recipients to copy the Corresponding Source along with the object code. If the place to copy @@ -595,7 +841,7 @@ copying facilities, provided you maintain clear directions next to the object co saying where to find the Corresponding Source. Regardless of what server hosts the Corresponding Source, you remain obligated to ensure that it is available for as long as needed to satisfy these requirements. -* e) Convey the object code using peer-to-peer transmission, provided you inform + e) Convey the object code using peer-to-peer transmission, provided you inform other peers where the object code and Corresponding Source of the work are being offered to the general public at no charge under subsection 6d. A separable portion of the object code, whose source code is excluded from the @@ -635,6 +881,7 @@ Corresponding Source conveyed, and Installation Information provided, in accord this section must be in a format that is publicly documented (and with an implementation available to the public in source code form), and must require no special password or key for unpacking, reading or copying. + 7. Additional Terms. "Additional permissions" are terms that supplement the terms of this License by making exceptions from one or more of its conditions. Additional permissions that are applicable @@ -651,19 +898,19 @@ which you have or can give appropriate copyright permission. Notwithstanding any other provision of this License, for material you add to a covered work, you may (if authorized by the copyright holders of that material) supplement the terms of this License with terms: -* a) Disclaiming warranty or limiting liability differently from the terms of sections + a) Disclaiming warranty or limiting liability differently from the terms of sections 15 and 16 of this License; or -* b) Requiring preservation of specified reasonable legal notices or author + b) Requiring preservation of specified reasonable legal notices or author attributions in that material or in the Appropriate Legal Notices displayed by works containing it; or -* c) Prohibiting misrepresentation of the origin of that material, or requiring that + c) Prohibiting misrepresentation of the origin of that material, or requiring that modified versions of such material be marked in reasonable ways as different from the original version; or -* d) Limiting the use for publicity purposes of names of licensors or authors of the + d) Limiting the use for publicity purposes of names of licensors or authors of the material; or -* e) Declining to grant rights under trademark law for use of some trade names, + e) Declining to grant rights under trademark law for use of some trade names, trademarks, or service marks; or -* f) Requiring indemnification of licensors and authors of that material by anyone + f) Requiring indemnification of licensors and authors of that material by anyone who conveys the material (or modified versions of it) with contractual assumptions of liability to the recipient, for any liability that these contractual assumptions directly impose on those licensors and authors. @@ -679,6 +926,7 @@ relevant source files, a statement of the additional terms that apply to those f notice indicating where to find the applicable terms. Additional terms, permissive or non-permissive, may be stated in the form of a separately written license, or stated as exceptions; the above requirements apply either way. + 8. Termination. You may not propagate or modify a covered work except as expressly provided under this License. Any attempt otherwise to propagate or modify it is void, and will @@ -698,6 +946,7 @@ Termination of your rights under this section does not terminate the licenses of who have received copies or rights from you under this License. If your rights have been terminated and not permanently reinstated, you do not qualify to receive new licenses for the same material under section 10. + 9. Acceptance Not Required for Having Copies. You are not required to accept this License in order to receive or run a copy of the Program. Ancillary propagation of a covered work occurring solely as a consequence of @@ -706,6 +955,7 @@ However, nothing other than this License grants you permission to propagate or m any covered work. These actions infringe copyright if you do not accept this License. Therefore, by modifying or propagating a covered work, you indicate your acceptance of this License to do so. + 10. Automatic Licensing of Downstream Recipients. Each time you convey a covered work, the recipient automatically receives a license from the original licensors, to run, modify and propagate that work, subject to this License. @@ -723,6 +973,7 @@ other charge for exercise of rights granted under this License, and you may not litigation (including a cross-claim or counterclaim in a lawsuit) alleging that any patent claim is infringed by making, using, selling, offering for sale, or importing the Program or any portion of it. + 11. Patents. A "contributor" is a copyright holder who authorizes use under this License of the Program or a work on which the Program is based. The work thus licensed is called the @@ -772,6 +1023,7 @@ that patent license was granted, prior to 28 March 2007. Nothing in this License shall be construed as excluding or limiting any implied license or other defenses to infringement that may otherwise be available to you under applicable patent law. + 12. No Surrender of Others' Freedom. If conditions are imposed on you (whether by court order, agreement or otherwise) that contradict the conditions of this License, they do not excuse you from the conditions of @@ -781,6 +1033,7 @@ you may not convey it at all. For example, if you agree to terms that obligate y collect a royalty for further conveying from those to whom you convey the Program, the only way you could satisfy both those terms and this License would be to refrain entirely from conveying the Program. + 13. Use with the GNU Affero General Public License. Notwithstanding any other provision of this License, you have permission to link or combine any covered work with a work licensed under version 3 of the GNU Affero @@ -788,6 +1041,7 @@ General Public License into a single combined work, and to convey the resulting The terms of this License will continue to apply to the part which is the covered work, but the special requirements of the GNU Affero General Public License, section 13, concerning interaction through a network will apply to the combination as such. + 14. Revised Versions of this License. The Free Software Foundation may publish revised and/or new versions of the GNU General Public License from time to time. Such new versions will be similar in spirit to the @@ -804,6 +1058,7 @@ version permanently authorizes you to choose that version for the Program. Later license versions may give you additional or different permissions. 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The Free Software Foundation may publish revised and/or new versions of the General Public License from time to time. Such new versions will be similar in spirit to the present version, but may differ in detail to address new problems or concerns. +Each version is given a distinguishing version number. If the Program specifies a version number of this License which applies to it and "any later version", you have the option of following the terms and conditions either of that version or of any later version published by the Free Software Foundation. If the Program does not specify a version number of this License, you may choose any version ever published by the Free Software Foundation. + +10. If you wish to incorporate parts of the Program into other free programs whose distribution conditions are different, write to the author to ask for permission. For software which is copyrighted by the Free Software Foundation, write to the Free Software Foundation; we sometimes make exceptions for this. Our decision will be guided by the two goals of preserving the free status of all derivatives of our free software and of promoting the sharing and reuse of software generally. +NO WARRANTY + +11. BECAUSE THE PROGRAM IS LICENSED FREE OF CHARGE, THERE IS NO WARRANTY FOR THE PROGRAM, TO THE EXTENT PERMITTED BY APPLICABLE LAW. EXCEPT WHEN OTHERWISE STATED IN WRITING THE COPYRIGHT HOLDERS AND/OR OTHER PARTIES PROVIDE THE PROGRAM "AS IS" WITHOUT WARRANTY OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE. THE ENTIRE RISK AS TO THE QUALITY AND PERFORMANCE OF THE PROGRAM IS WITH YOU. SHOULD THE PROGRAM PROVE DEFECTIVE, YOU ASSUME THE COST OF ALL NECESSARY SERVICING, REPAIR OR CORRECTION. + +12. IN NO EVENT UNLESS REQUIRED BY APPLICABLE LAW OR AGREED TO IN WRITING WILL ANY COPYRIGHT HOLDER, OR ANY OTHER PARTY WHO MAY MODIFY AND/OR REDISTRIBUTE THE PROGRAM AS PERMITTED ABOVE, BE LIABLE TO YOU FOR DAMAGES, INCLUDING ANY GENERAL, SPECIAL, INCIDENTAL OR CONSEQUENTIAL DAMAGES ARISING OUT OF THE USE OR INABILITY TO USE THE PROGRAM (INCLUDING BUT NOT LIMITED TO LOSS OF DATA OR DATA BEING RENDERED INACCURATE OR LOSSES SUSTAINED BY YOU OR THIRD PARTIES OR A FAILURE OF THE PROGRAM TO OPERATE WITH ANY OTHER PROGRAMS), EVEN IF SUCH HOLDER OR OTHER PARTY HAS BEEN ADVISED OF THE POSSIBILITY OF SUCH DAMAGES. diff --git a/dbReports/.pylintrc b/dbReports/.pylintrc index 7a7ee07b..c7cd3b89 100644 --- a/dbReports/.pylintrc +++ b/dbReports/.pylintrc @@ -29,7 +29,7 @@ cache-size=500 [MESSAGES CONTROL] -# C0111 Missing docstring +# C0111 Missing docstring # I0011 Warning locally suppressed using disable-msg # I0012 Warning locally suppressed using disable-msg # W0704 Except doesn't do anything Used when an except clause does nothing but "pass" and there is no "else" clause @@ -47,7 +47,7 @@ disable=C0111,I0011,I0012,W0704,W0142,W0212,W0232,W0613,W0702,R0201,C0103,C0301, # Set the output format. Available formats are text, parseable, colorized, msvs # (visual studio) and html -output-format=parseable +output-format=colorized # Include message's id in output include-ids=yes diff --git a/dbReports/CMakeLists.txt b/dbReports/CMakeLists.txt index 5db8c404..b9418a7e 100644 --- a/dbReports/CMakeLists.txt +++ b/dbReports/CMakeLists.txt @@ -91,24 +91,10 @@ install(DIRECTORY DESTINATION /var/log/ion DIRECTORY_PERMISSIONS OWNER_READ OWNE install(PROGRAMS iondb/bin/ionCrawler DESTINATION /etc/init.d) install(PROGRAMS iondb/bin/ionJobServer DESTINATION /etc/init.d) install(PROGRAMS iondb/bin/ionPlugin DESTINATION /etc/init.d) - -# celeryd and celerybeat init and config files: subject to relocation in future - -install(PROGRAMS celery/init.d/celeryd DESTINATION /etc/init.d) -install(PROGRAMS celery/init.d/celerybeat DESTINATION /etc/init.d) -configure_file ( - "${PROJECT_SOURCE_DIR}/celery/config/celeryd.in" - "${PROJECT_BINARY_DIR}/celery/config/celeryd" @ONLY -) -install(FILES "${PROJECT_BINARY_DIR}/celery/config/celeryd" DESTINATION /etc/default) -configure_file ( - "${PROJECT_SOURCE_DIR}/celery/config/celerybeat.in" - "${PROJECT_BINARY_DIR}/celery/config/celerybeat" @ONLY -) -install(FILES "${PROJECT_BINARY_DIR}/celery/config/celerybeat" DESTINATION /etc/default) - +install(PROGRAMS celery/init/celeryd.conf DESTINATION /etc/init) +install(PROGRAMS celery/init/celerybeat.conf DESTINATION /etc/init) +install(PROGRAMS celery/init/DjangoFTP.conf DESTINATION /etc/init) install(PROGRAMS manage.py DESTINATION ${ION_INSTALL_PREFIX}) -#install(FILES celeryconfig.py DESTINATION ${ION_INSTALL_PREFIX}) configure_file ( "${PROJECT_SOURCE_DIR}/apacheconfig/torrent-server-config.conf.in" @@ -159,6 +145,8 @@ set(CPACK_GENERATOR "DEB") include(../buildTools/cmake/CMakeLists.cpack.txt) +set(CPACK_DEBIAN_PACKAGE_PREDEPENDS "debconf") + #% nice to automate this set(CPACK_DEBIAN_PACKAGE_DEPENDS "python, avahi-daemon, @@ -169,10 +157,12 @@ set(CPACK_DEBIAN_PACKAGE_DEPENDS "python, pdftk, postgresql, php5, + apache2, libapache2-mod-wsgi (>= 3.3), libapache2-mod-gnutls, apache2-utils, ssl-cert, + dmidecode, python-avahi, python-celery (>= 3.1.6), python-django (>= 1.6.1), @@ -196,13 +186,18 @@ set(CPACK_DEBIAN_PACKAGE_DEPENDS "python, python-feedparser, python-boto (>=2.20.1), python-oauthlib, + python-gobject, + python-gnupg, + python-pyftpdlib, memcached, python-memcache, python-dev, rabbitmq-server (>= 3.2.0), ion-analysis (>=3.5.15), megacli, - python-apt") + python-apt, + ion-tsconfig, + coreutils") set(CPACK_PACKAGE_DESCRIPTION "This package contains the Torrent Browser and related programs including the job server and run crawler.") set(CPACK_DEBIAN_PACKAGE_CONTROL_EXTRA @@ -211,5 +206,6 @@ set(CPACK_DEBIAN_PACKAGE_CONTROL_EXTRA ${CMAKE_CURRENT_BINARY_DIR}/debian/prerm ${CMAKE_CURRENT_BINARY_DIR}/debian/postrm ${CMAKE_CURRENT_BINARY_DIR}/debian/triggers + ${PROJECT_SOURCE_DIR}/debian/templates ) include(CPack) diff --git a/dbReports/apacheconfig/torrent-server-config.conf.in b/dbReports/apacheconfig/torrent-server-config.conf.in index 71d2a511..15b8bf94 100644 --- a/dbReports/apacheconfig/torrent-server-config.conf.in +++ b/dbReports/apacheconfig/torrent-server-config.conf.in @@ -20,6 +20,7 @@ WSGIDaemonProcess iontorrent threads=6 processes=16 maximum-requests=500 shutdow WSGIScriptAlias /data @ION_INSTALL_PREFIX@/iondb/wsgi.py WSGIScriptAlias /configure @ION_INSTALL_PREFIX@/iondb/wsgi.py WSGIScriptAlias /rundb @ION_INSTALL_PREFIX@/iondb/wsgi.py +WSGIScriptAlias /security @ION_INSTALL_PREFIX@/iondb/wsgi.py WSGIScriptAlias /plan @ION_INSTALL_PREFIX@/iondb/wsgi.py WSGIScriptAlias /sample @ION_INSTALL_PREFIX@/iondb/wsgi.py WSGIScriptAlias /monitor @ION_INSTALL_PREFIX@/iondb/wsgi.py @@ -61,16 +62,6 @@ WSGIScriptAlias /admin @ION_INSTALL_PREFIX@/iondb/wsgi.py Allow from 127.0.0.0/8 Allow from ::1 Satisfy Any - - # CORS for cross domain json requests - # - makes API accessible from other sites - - - SetEnvIf Origin ":" IS_CORS - Header set Access-Control-Allow-Origin "*" env=IS_CORS - Header set Access-Control-Allow-Credentials "true" env=IS_CORS - - diff --git a/dbReports/celery/config/celerybeat.in b/dbReports/celery/config/celerybeat.in deleted file mode 100644 index 90d691ce..00000000 --- a/dbReports/celery/config/celerybeat.in +++ /dev/null @@ -1,18 +0,0 @@ -# Copyright (C) 2014 Ion Torrent Systems, Inc. All Rights Reserved - -CELERY_BIN="celery" -CELERY_APP="iondb" - -CELERYBEAT_CHDIR="@ION_INSTALL_PREFIX@" - -# Extra arguments to celerybeat -CELERYBEAT_OPTS="--schedule=/var/run/celery/celerybeat-schedule" - -export DJANGO_SETTINGS_MODULE="iondb.settings" - -CELERYBEAT_LOG_LEVEL=INFO -CELERYBEAT_LOG_FILE="/var/log/ion/celerybeat.log" -CELERYBEAT_PID_FILE="/var/run/celery/celerybeat.pid" - -CELERY_CREATE_DIRS=1 - diff --git a/dbReports/celery/config/celeryd.in b/dbReports/celery/config/celeryd.in deleted file mode 100644 index 87f86698..00000000 --- a/dbReports/celery/config/celeryd.in +++ /dev/null @@ -1,48 +0,0 @@ -# Copyright (C) 2014 Ion Torrent Systems, Inc. All Rights Reserved - -# Name of the projects settings module. (set in celery.py) -export DJANGO_SETTINGS_MODULE="iondb.settings" -export PATH="${PATH:+$PATH:}/usr/sbin:/sbin" - -# Name of nodes to start, here we have a single node -# Edits to NODES need to be in iondb/rundb/configure/views.py - services tab reporting -CELERYD_NODES="w1 plugins periodic slowlane transfer diskutil dmprocess dmmanage" - -# Where to chdir at start. -CELERYD_CHDIR="@ION_INSTALL_PREFIX@" - -CELERY_APP="iondb" - -# Default log level -CELERYD_LOG_LEVEL=INFO - -# The following abbreviations will be expanded in file names below -# * %n -> node name -# * %h -> host name -# Extra arguments to celeryd -CELERYD_OPTS="-Ofair --event --time-limit=21600" -CELERYD_OPTS="$CELERYD_OPTS --queue:w1=w1 --concurrency:w1=4" -CELERYD_OPTS="$CELERYD_OPTS --queue:plugins=plugins --concurrency:plugins=2" -CELERYD_OPTS="$CELERYD_OPTS --queue:periodic=periodic --concurrency:periodic=6" -CELERYD_OPTS="$CELERYD_OPTS --queue:slowlane=slowlane --concurrency:slowlane=1" -CELERYD_OPTS="$CELERYD_OPTS --queue:transfer=transfer --concurrency:transfer=1" -CELERYD_OPTS="$CELERYD_OPTS --queue:diskutil=diskutil --concurrency:diskutil=2" -CELERYD_OPTS="$CELERYD_OPTS --queue:dmprocess=dmprocess --concurrency:dmprocess=8" -CELERYD_OPTS="$CELERYD_OPTS --queue:dmmanage=dmmanage --concurrency:dmmanage=8" - -# %N will be replaced with the first part of the nodename. -CELERYD_LOG_FILE="/var/log/ion/celery_%n.log" -CELERYD_PID_FILE="/var/run/celery/celery_%n.pid" - -# Workers should run as an unprivileged user. -# You need to create this user manually (or you can choose -# a user/group combination that already exists, e.g. nobody). -CELERYD_USER="root" -CELERYD_GROUP="root" -export C_FORCE_ROOT="true" - -# If enabled pid and log directories will be created if missing, -# and owned by the userid/group configured. -CELERY_CREATE_DIRS=1 - - diff --git a/dbReports/celery/init.d/celerybeat b/dbReports/celery/init.d/celerybeat deleted file mode 100644 index fa4cd68b..00000000 --- a/dbReports/celery/init.d/celerybeat +++ /dev/null @@ -1,318 +0,0 @@ -#!/bin/bash -# ========================================================= -# celerybeat - Starts the Celery periodic task scheduler. -# ========================================================= -# -# :Usage: /etc/init.d/celerybeat {start|stop|force-reload|restart|try-restart|status} -# :Configuration file: /etc/default/celerybeat or /etc/default/celeryd -# -# See http://docs.celeryproject.org/en/latest/tutorials/daemonizing.html#generic-init-scripts - -### BEGIN INIT INFO -# Provides: celerybeat -# Required-Start: $network $local_fs $remote_fs -# Required-Stop: $network $local_fs $remote_fs -# Default-Start: 2 3 4 5 -# Default-Stop: 0 1 6 -# Short-Description: celery periodic task scheduler -### END INIT INFO - -# Cannot use set -e/bash -e since the kill -0 command will abort -# abnormally in the absence of a valid process ID. -#set -e -VERSION=10.1 -echo "celery init v${VERSION}." - -if [ $(id -u) -ne 0 ]; then - echo "Error: This program can only be used by the root user." - echo " Unpriviliged users must use 'celery beat --detach'" - exit 1 -fi - - -# May be a runlevel symlink (e.g. S02celeryd) -if [ -L "$0" ]; then - SCRIPT_FILE=$(readlink "$0") -else - SCRIPT_FILE="$0" -fi -SCRIPT_NAME="$(basename "$SCRIPT_FILE")" - -# /etc/init.d/celerybeat: start and stop the celery periodic task scheduler daemon. - -# Make sure executable configuration script is owned by root -_config_sanity() { - local path="$1" - local owner=$(ls -ld "$path" | awk '{print $3}') - local iwgrp=$(ls -ld "$path" | cut -b 6) - local iwoth=$(ls -ld "$path" | cut -b 9) - - if [ "$(id -u $owner)" != "0" ]; then - echo "Error: Config script '$path' must be owned by root!" - echo - echo "Resolution:" - echo "Review the file carefully and make sure it has not been " - echo "modified with mailicious intent. When sure the " - echo "script is safe to execute with superuser privileges " - echo "you can change ownership of the script:" - echo " $ sudo chown root '$path'" - exit 1 - fi - - if [ "$iwoth" != "-" ]; then # S_IWOTH - echo "Error: Config script '$path' cannot be writable by others!" - echo - echo "Resolution:" - echo "Review the file carefully and make sure it has not been " - echo "modified with malicious intent. When sure the " - echo "script is safe to execute with superuser privileges " - echo "you can change the scripts permissions:" - echo " $ sudo chmod 640 '$path'" - exit 1 - fi - if [ "$iwgrp" != "-" ]; then # S_IWGRP - echo "Error: Config script '$path' cannot be writable by group!" - echo - echo "Resolution:" - echo "Review the file carefully and make sure it has not been " - echo "modified with malicious intent. When sure the " - echo "script is safe to execute with superuser privileges " - echo "you can change the scripts permissions:" - echo " $ sudo chmod 640 '$path'" - exit 1 - fi -} - -scripts="" - -if test -f /etc/default/celeryd; then - scripts="/etc/default/celeryd" - _config_sanity /etc/default/celeryd - . /etc/default/celeryd -fi - -EXTRA_CONFIG="/etc/default/${SCRIPT_NAME}" -if test -f "$EXTRA_CONFIG"; then - scripts="$scripts, $EXTRA_CONFIG" - _config_sanity "$EXTRA_CONFIG" - . "$EXTRA_CONFIG" -fi - -echo "Using configuration: $scripts" - -CELERY_BIN=${CELERY_BIN:-"celery"} -DEFAULT_USER="celery" -DEFAULT_PID_FILE="/var/run/celery/beat.pid" -DEFAULT_LOG_FILE="/var/log/celery/beat.log" -DEFAULT_LOG_LEVEL="INFO" -DEFAULT_CELERYBEAT="$CELERY_BIN beat" - -CELERYBEAT=${CELERYBEAT:-$DEFAULT_CELERYBEAT} -CELERYBEAT_LOG_LEVEL=${CELERYBEAT_LOG_LEVEL:-${CELERYBEAT_LOGLEVEL:-$DEFAULT_LOG_LEVEL}} - -# Sets --app argument for CELERY_BIN -CELERY_APP_ARG="" -if [ ! -z "$CELERY_APP" ]; then - CELERY_APP_ARG="--app=$CELERY_APP" -fi - -CELERYBEAT_USER=${CELERYBEAT_USER:-${CELERYD_USER:-$DEFAULT_USER}} - -# Set CELERY_CREATE_DIRS to always create log/pid dirs. -CELERY_CREATE_DIRS=${CELERY_CREATE_DIRS:-0} -CELERY_CREATE_RUNDIR=$CELERY_CREATE_DIRS -CELERY_CREATE_LOGDIR=$CELERY_CREATE_DIRS -if [ -z "$CELERYBEAT_PID_FILE" ]; then - CELERYBEAT_PID_FILE="$DEFAULT_PID_FILE" - CELERY_CREATE_RUNDIR=1 -fi -if [ -z "$CELERYBEAT_LOG_FILE" ]; then - CELERYBEAT_LOG_FILE="$DEFAULT_LOG_FILE" - CELERY_CREATE_LOGDIR=1 -fi - -export CELERY_LOADER=default - -CELERYBEAT_OPTS="$CELERYBEAT_OPTS -f $CELERYBEAT_LOG_FILE -l $CELERYBEAT_LOG_LEVEL" - -if [ -n "$2" ]; then - CELERYBEAT_OPTS="$CELERYBEAT_OPTS $2" -fi - -CELERYBEAT_LOG_DIR=`dirname $CELERYBEAT_LOG_FILE` -CELERYBEAT_PID_DIR=`dirname $CELERYBEAT_PID_FILE` - -# Extra start-stop-daemon options, like user/group. - -CELERYBEAT_CHDIR=${CELERYBEAT_CHDIR:-$CELERYD_CHDIR} -if [ -n "$CELERYBEAT_CHDIR" ]; then - DAEMON_OPTS="$DAEMON_OPTS --workdir=$CELERYBEAT_CHDIR" -fi - - -export PATH="${PATH:+$PATH:}/usr/sbin:/sbin" - -check_dev_null() { - if [ ! -c /dev/null ]; then - echo "/dev/null is not a character device!" - exit 75 # EX_TEMPFAIL - fi -} - -maybe_die() { - if [ $? -ne 0 ]; then - echo "Exiting: $*" - exit 77 # EX_NOPERM - fi -} - -create_default_dir() { - if [ ! -d "$1" ]; then - echo "- Creating default directory: '$1'" - mkdir -p "$1" - maybe_die "Couldn't create directory $1" - echo "- Changing permissions of '$1' to 02755" - chmod 02755 "$1" - maybe_die "Couldn't change permissions for $1" - if [ -n "$CELERYBEAT_USER" ]; then - echo "- Changing owner of '$1' to '$CELERYBEAT_USER'" - chown "$CELERYBEAT_USER" "$1" - maybe_die "Couldn't change owner of $1" - fi - if [ -n "$CELERYBEAT_GROUP" ]; then - echo "- Changing group of '$1' to '$CELERYBEAT_GROUP'" - chgrp "$CELERYBEAT_GROUP" "$1" - maybe_die "Couldn't change group of $1" - fi - fi -} - -check_paths() { - if [ $CELERY_CREATE_LOGDIR -eq 1 ]; then - create_default_dir "$CELERYBEAT_LOG_DIR" - fi - if [ $CELERY_CREATE_RUNDIR -eq 1 ]; then - create_default_dir "$CELERYBEAT_PID_DIR" - fi -} - - -create_paths () { - create_default_dir "$CELERYBEAT_LOG_DIR" - create_default_dir "$CELERYBEAT_PID_DIR" -} - - -wait_pid () { - pid=$1 - forever=1 - i=0 - while [ $forever -gt 0 ]; do - kill -0 $pid 1>/dev/null 2>&1 - if [ $? -eq 1 ]; then - echo "OK" - forever=0 - else - kill -TERM "$pid" - i=$((i + 1)) - if [ $i -gt 60 ]; then - echo "ERROR" - echo "Timed out while stopping (30s)" - forever=0 - else - sleep 0.5 - fi - fi - done -} - - -stop_beat () { - echo -n "Stopping ${SCRIPT_NAME}... " - if [ -f "$CELERYBEAT_PID_FILE" ]; then - wait_pid $(cat "$CELERYBEAT_PID_FILE") - else - echo "NOT RUNNING" - fi -} - -_chuid () { - su "$CELERYBEAT_USER" -c "$CELERYBEAT $*" -} - -start_beat () { - echo "Starting ${SCRIPT_NAME}..." - _chuid $CELERY_APP_ARG $CELERYBEAT_OPTS $DAEMON_OPTS --detach \ - --pidfile="$CELERYBEAT_PID_FILE" -} - - -check_status () { - local failed= - local pid_file=$CELERYBEAT_PID_FILE - if [ ! -e $pid_file ]; then - echo "${SCRIPT_NAME} is up: no pid file found" - failed=true - elif [ ! -r $pid_file ]; then - echo "${SCRIPT_NAME} is in unknown state, user cannot read pid file." - failed=true - else - local pid=`cat "$pid_file"` - local cleaned_pid=`echo "$pid" | sed -e 's/[^0-9]//g'` - if [ -z "$pid" ] || [ "$cleaned_pid" != "$pid" ]; then - echo "${SCRIPT_NAME}: bad pid file ($pid_file)" - failed=true - else - local failed= - kill -0 $pid 2> /dev/null || failed=true - if [ "$failed" ]; then - echo "${SCRIPT_NAME} (pid $pid) is down, but pid file exists!" - failed=true - else - echo "${SCRIPT_NAME} (pid $pid) is up..." - fi - fi - fi - - [ "$failed" ] && exit 1 || exit 0 -} - - -case "$1" in - start) - check_dev_null - check_paths - start_beat - ;; - stop) - check_paths - stop_beat - ;; - reload|force-reload) - echo "Use start+stop" - ;; - status) - check_status - ;; - restart) - echo "Restarting celery periodic task scheduler" - check_paths - stop_beat - check_dev_null - start_beat - ;; - create-paths) - check_dev_null - create_paths - ;; - check-paths) - check_dev_null - check_paths - ;; - *) - echo "Usage: /etc/init.d/${SCRIPT_NAME} {start|stop|restart|create-paths|status}" - exit 64 # EX_USAGE - ;; -esac - -exit 0 diff --git a/dbReports/celery/init.d/celeryd b/dbReports/celery/init.d/celeryd deleted file mode 100644 index 2a6bcf50..00000000 --- a/dbReports/celery/init.d/celeryd +++ /dev/null @@ -1,397 +0,0 @@ -#!/bin/sh -e -# ============================================ -# celeryd - Starts the Celery worker daemon. -# ============================================ -# -# :Usage: /etc/init.d/celeryd {start|stop|force-reload|restart|try-restart|status} -# :Configuration file: /etc/default/celeryd -# -# See http://docs.celeryproject.org/en/latest/tutorials/daemonizing.html#generic-init-scripts - - -### BEGIN INIT INFO -# Provides: celeryd -# Required-Start: $network $local_fs $remote_fs -# Required-Stop: $network $local_fs $remote_fs -# Default-Start: 2 3 4 5 -# Default-Stop: 0 1 6 -# Short-Description: celery task worker daemon -### END INIT INFO -# -# -# To implement separate init scripts, copy this script and give it a different -# name: -# I.e., if my new application, "little-worker" needs an init, I -# should just use: -# -# cp /etc/init.d/celeryd /etc/init.d/little-worker -# -# You can then configure this by manipulating /etc/default/little-worker. -# -VERSION=10.1 -echo "celery init v${VERSION}." -if [ $(id -u) -ne 0 ]; then - echo "Error: This program can only be used by the root user." - echo " Unprivileged users must use the 'celery multi' utility, " - echo " or 'celery worker --detach'." - exit 1 -fi - - -# Can be a runlevel symlink (e.g. S02celeryd) -if [ -L "$0" ]; then - SCRIPT_FILE=$(readlink "$0") -else - SCRIPT_FILE="$0" -fi -SCRIPT_NAME="$(basename "$SCRIPT_FILE")" - -DEFAULT_USER="celery" -DEFAULT_PID_FILE="/var/run/celery/%n.pid" -DEFAULT_LOG_FILE="/var/log/celery/%n.log" -DEFAULT_LOG_LEVEL="INFO" -DEFAULT_NODES="celery" -DEFAULT_CELERYD="-m celery worker --detach" - -CELERY_DEFAULTS=${CELERY_DEFAULTS:-"/etc/default/${SCRIPT_NAME}"} - -# Make sure executable configuration script is owned by root -_config_sanity() { - local path="$1" - local owner=$(ls -ld "$path" | awk '{print $3}') - local iwgrp=$(ls -ld "$path" | cut -b 6) - local iwoth=$(ls -ld "$path" | cut -b 9) - - if [ "$(id -u $owner)" != "0" ]; then - echo "Error: Config script '$path' must be owned by root!" - echo - echo "Resolution:" - echo "Review the file carefully and make sure it has not been " - echo "modified with mailicious intent. When sure the " - echo "script is safe to execute with superuser privileges " - echo "you can change ownership of the script:" - echo " $ sudo chown root '$path'" - exit 1 - fi - - if [ "$iwoth" != "-" ]; then # S_IWOTH - echo "Error: Config script '$path' cannot be writable by others!" - echo - echo "Resolution:" - echo "Review the file carefully and make sure it has not been " - echo "modified with malicious intent. When sure the " - echo "script is safe to execute with superuser privileges " - echo "you can change the scripts permissions:" - echo " $ sudo chmod 640 '$path'" - exit 1 - fi - if [ "$iwgrp" != "-" ]; then # S_IWGRP - echo "Error: Config script '$path' cannot be writable by group!" - echo - echo "Resolution:" - echo "Review the file carefully and make sure it has not been " - echo "modified with malicious intent. When sure the " - echo "script is safe to execute with superuser privileges " - echo "you can change the scripts permissions:" - echo " $ sudo chmod 640 '$path'" - exit 1 - fi -} - -if [ -f "$CELERY_DEFAULTS" ]; then - _config_sanity "$CELERY_DEFAULTS" - echo "Using config script: $CELERY_DEFAULTS" - . "$CELERY_DEFAULTS" -fi - -# Sets --app argument for CELERY_BIN -CELERY_APP_ARG="" -if [ ! -z "$CELERY_APP" ]; then - CELERY_APP_ARG="--app=$CELERY_APP" -fi - -CELERYD_USER=${CELERYD_USER:-$DEFAULT_USER} - -# Set CELERY_CREATE_DIRS to always create log/pid dirs. -CELERY_CREATE_DIRS=${CELERY_CREATE_DIRS:-0} -CELERY_CREATE_RUNDIR=$CELERY_CREATE_DIRS -CELERY_CREATE_LOGDIR=$CELERY_CREATE_DIRS -if [ -z "$CELERYD_PID_FILE" ]; then - CELERYD_PID_FILE="$DEFAULT_PID_FILE" - CELERY_CREATE_RUNDIR=1 -fi -if [ -z "$CELERYD_LOG_FILE" ]; then - CELERYD_LOG_FILE="$DEFAULT_LOG_FILE" - CELERY_CREATE_LOGDIR=1 -fi - -CELERYD_LOG_LEVEL=${CELERYD_LOG_LEVEL:-${CELERYD_LOGLEVEL:-$DEFAULT_LOG_LEVEL}} -CELERY_BIN=${CELERY_BIN:-"celery"} -CELERYD_MULTI=${CELERYD_MULTI:-"$CELERY_BIN multi"} -CELERYD_NODES=${CELERYD_NODES:-$DEFAULT_NODES} - -export CELERY_LOADER=default - -if [ -n "$2" ]; then - CELERYD_OPTS="$CELERYD_OPTS $2" -fi - -CELERYD_LOG_DIR=`dirname $CELERYD_LOG_FILE` -CELERYD_PID_DIR=`dirname $CELERYD_PID_FILE` - -# Extra start-stop-daemon options, like user/group. -if [ -n "$CELERYD_CHDIR" ]; then - DAEMON_OPTS="$DAEMON_OPTS --workdir=$CELERYD_CHDIR" -fi - - -check_dev_null() { - if [ ! -c /dev/null ]; then - echo "/dev/null is not a character device!" - exit 75 # EX_TEMPFAIL - fi -} - - -maybe_die() { - if [ $? -ne 0 ]; then - echo "Exiting: $* (errno $?)" - exit 77 # EX_NOPERM - fi -} - -create_default_dir() { - if [ ! -d "$1" ]; then - echo "- Creating default directory: '$1'" - mkdir -p "$1" - maybe_die "Couldn't create directory $1" - echo "- Changing permissions of '$1' to 02755" - chmod 02755 "$1" - maybe_die "Couldn't change permissions for $1" - if [ -n "$CELERYD_USER" ]; then - echo "- Changing owner of '$1' to '$CELERYD_USER'" - chown "$CELERYD_USER" "$1" - maybe_die "Couldn't change owner of $1" - fi - if [ -n "$CELERYD_GROUP" ]; then - echo "- Changing group of '$1' to '$CELERYD_GROUP'" - chgrp "$CELERYD_GROUP" "$1" - maybe_die "Couldn't change group of $1" - fi - fi -} - - -check_paths() { - if [ $CELERY_CREATE_LOGDIR -eq 1 ]; then - create_default_dir "$CELERYD_LOG_DIR" - fi - if [ $CELERY_CREATE_RUNDIR -eq 1 ]; then - create_default_dir "$CELERYD_PID_DIR" - fi - # Touch a tracking file in the /results directory. - touch /results/.headnode_$(cat /etc/hostname) || true -} - -create_paths() { - create_default_dir "$CELERYD_LOG_DIR" - create_default_dir "$CELERYD_PID_DIR" -} - -export PATH="${PATH:+$PATH:}/usr/sbin:/sbin" - - -_get_pidfiles () { - # note: multi < 3.1.14 output to stderr, not stdout, hence the redirect. - ${CELERYD_MULTI} expand "${CELERYD_PID_FILE}" ${CELERYD_NODES} 2>&1 -} - - -_get_pids() { - found_pids=0 - my_exitcode=0 - - for pidfile in $(_get_pidfiles); do - local pid=`cat "$pidfile"` - local cleaned_pid=`echo "$pid" | sed -e 's/[^0-9]//g'` - if [ -z "$pid" ] || [ "$cleaned_pid" != "$pid" ]; then - echo "bad pid file ($pidfile)" - one_failed=true - my_exitcode=1 - else - found_pids=1 - echo "$pid" - fi - - if [ $found_pids -eq 0 ]; then - echo "${SCRIPT_NAME}: All nodes down" - exit $my_exitcode - fi - done -} - - -_chuid () { - su "$CELERYD_USER" -c "$CELERYD_MULTI $*" -} - - -start_workers () { - if [ ! -z "$CELERYD_ULIMIT" ]; then - ulimit $CELERYD_ULIMIT - fi - _chuid $* start $CELERYD_NODES $DAEMON_OPTS \ - --pidfile="$CELERYD_PID_FILE" \ - --logfile="$CELERYD_LOG_FILE" \ - --loglevel="$CELERYD_LOG_LEVEL" \ - $CELERY_APP_ARG \ - $CELERYD_OPTS -} - - -dryrun () { - (C_FAKEFORK=1 start_workers --verbose) -} - - -stop_workers () { - _chuid stopwait $CELERYD_NODES --pidfile="$CELERYD_PID_FILE" -} - - -restart_workers () { - _chuid restart $CELERYD_NODES $DAEMON_OPTS \ - --pidfile="$CELERYD_PID_FILE" \ - --logfile="$CELERYD_LOG_FILE" \ - --loglevel="$CELERYD_LOG_LEVEL" \ - $CELERY_APP_ARG \ - $CELERYD_OPTS -} - - -kill_workers() { - _chuid kill $CELERYD_NODES --pidfile="$CELERYD_PID_FILE" -} - - -restart_workers_graceful () { - echo "WARNING: Use with caution in production" - echo "The workers will attempt to restart, but they may not be able to." - local worker_pids= - worker_pids=`_get_pids` - [ "$one_failed" ] && exit 1 - - for worker_pid in $worker_pids; do - local failed= - kill -HUP $worker_pid 2> /dev/null || failed=true - if [ "$failed" ]; then - echo "${SCRIPT_NAME} worker (pid $worker_pid) could not be restarted" - one_failed=true - else - echo "${SCRIPT_NAME} worker (pid $worker_pid) received SIGHUP" - fi - done - - [ "$one_failed" ] && exit 1 || exit 0 -} - - -check_status () { - my_exitcode=0 - found_pids=0 - - local one_failed= - for pidfile in $(_get_pidfiles); do - if [ ! -r $pidfile ]; then - echo "${SCRIPT_NAME} down: no pidfiles found" - one_failed=true - break - fi - - local node=`basename "$pidfile" .pid` - local pid=`cat "$pidfile"` - local cleaned_pid=`echo "$pid" | sed -e 's/[^0-9]//g'` - if [ -z "$pid" ] || [ "$cleaned_pid" != "$pid" ]; then - echo "bad pid file ($pidfile)" - one_failed=true - else - local failed= - kill -0 $pid 2> /dev/null || failed=true - if [ "$failed" ]; then - echo "${SCRIPT_NAME} (node $node) (pid $pid) is down, but pidfile exists!" - one_failed=true - else - echo "${SCRIPT_NAME} (node $node) (pid $pid) is up..." - fi - fi - done - - [ "$one_failed" ] && exit 1 || exit 0 -} - - -case "$1" in - start) - check_dev_null - check_paths - start_workers - ;; - - stop) - check_dev_null - check_paths - stop_workers - ;; - - reload|force-reload) - echo "Use restart" - ;; - - status) - check_status - ;; - - restart) - check_dev_null - check_paths - restart_workers - ;; - - graceful) - check_dev_null - restart_workers_graceful - ;; - - kill) - check_dev_null - kill_workers - ;; - - dryrun) - check_dev_null - dryrun - ;; - - try-restart) - check_dev_null - check_paths - restart_workers - ;; - - create-paths) - check_dev_null - create_paths - ;; - - check-paths) - check_dev_null - check_paths - ;; - - *) - echo "Usage: /etc/init.d/${SCRIPT_NAME} {start|stop|restart|graceful|kill|dryrun|create-paths}" - exit 64 # EX_USAGE - ;; -esac - -exit 0 diff --git a/dbReports/celery/init.d/celeryd.new b/dbReports/celery/init.d/celeryd.new deleted file mode 100644 index b8c87b26..00000000 --- a/dbReports/celery/init.d/celeryd.new +++ /dev/null @@ -1,188 +0,0 @@ -#!/bin/sh -# Copyright (C) 2011 Ion Torrent Systems, Inc. All Rights Reserved -### BEGIN INIT INFO -# Provides: celeryd -# Required-Start: $network $local_fs $remote_fs postgresql -# Required-Stop: $network $local_fs $remote_fs postgresql -# Default-Start: 2 3 4 5 -# Default-Stop: 0 1 6 -# Short-Description: celery task worker daemon -### END INIT INFO - -# Name of the projects settings module. -export DJANGO_SETTINGS_MODULE="iondb.settings" -export PATH="${PATH:+$PATH:}/usr/sbin:/sbin" - -# Name of nodes to start, here we have a single node -# Edits to NODES need to be in iondb/rundb/configure/views.py - services tab reporting -CELERYD_NODES="w1 plugins periodic slowlane transfer diskutil" - -# Where to chdir at start. -CELERYD_CHDIR="/opt/ion" - -# How to call "manage.py celeryd_multi" -CELERY_MULTI="$CELERYD_CHDIR/manage.py celery multi" - -# The following abbreviations will be expanded in file names below -# * %n -> node name -# * %h -> host name -# Extra arguments to celeryd -CELERYD_OPTS="--events " -CELERYD_OPTS="$CELERYD_OPTS --loglevel=INFO" - -CELERYD_REQ="--pidfile=/var/run/celery/celery_%n.pid" -CELERYD_REQ="$CELERYD_REQ --logfile=/var/log/ion/celery_%n.log" - -CELERYBEAT="$CELERYD_CHDIR/manage.py celery beat" -CELERYBEAT_PID_FILE="/var/run/celery/celerybeat.pid" -CELERYBEAT_SCHEDULE_FILE="/var/run/celery/celerybeat-schedule" - -if [ -n "$2" ]; then - CELERYD_OPTS="$CELERYD_OPTS $2" -fi - -check_dev_null() { - if [ ! -c /dev/null ]; then - echo "/dev/null is not a character device!" - exit 1 - fi -} - -check_privsep_dir() { - # Create the PrivSep empty dir if necessary - if [ ! -d /var/run/celery ]; then - mkdir /var/run/celery - fi - chown -R ionian:ionadmin /var/run/celery - chmod 0775 /var/run/celery -} - -wait_pid () { - pid=$1 - forever=1 - i=0 - while [ $forever -gt 0 ]; do - kill -0 $pid 1>/dev/null 2>&1 - if [ $? -eq 1 ]; then - echo "OK" - forever=0 - else - i=$((i + 1)) - if [ $i -gt 60 ]; then - echo "Timed out while stopping (30s)" - kill -9 "$pid" - forever=0 - else - kill -TERM "$pid" - sleep 0.5 - fi - fi - done -} - -stop_beat () { - echo -n "Stopping celerybeat... " - if [ -f "$CELERYBEAT_PID_FILE" ]; then - wait_pid $(cat "$CELERYBEAT_PID_FILE") - else - echo "celerybeat not running" - fi -} - -start_beat () { - echo "Starting celerybeat..." - $CELERYBEAT $CELERYBEAT_OPTS \ - --pidfile="$CELERYBEAT_PID_FILE" \ - --logfile="/var/log/ion/celerybeat.log" \ - --loglevel=INFO \ - --schedule="$CELERYBEAT_SCHEDULE_FILE" \ - --detach -} - -multi_command () { - $CELERY_MULTI $1 $CELERYD_NODES \ - $CELERYD_OPTS \ - $CELERYD_REQ \ - --concurrency:w1=4 \ - --concurrency:plugins=2 \ - --queue:plugins=plugins \ - --concurrency:periodic=6 \ - --queue:periodic=periodic \ - --concurrency:slowlane=1 \ - --queue:slowlane=slowlane \ - --concurrency:transfer=1 \ - --queue:transfer=transfer \ - --queue:diskutil=diskutil \ - --concurrency:diskutil=2 -} - -start_workers () { - multi_command start; - start_beat; -} - -restart_workers () { - stop_beat; - multi_command restart; - start_beat; -} - -stop_workers () { - stop_beat; - multi_command stopwait; - if ps aux|grep "/opt/ion/manage.py celery"|grep -q -v grep; then - echo "There are still processes around!" - fi -} - -check_status () { - pids=`cat /var/run/celery/celery_*.pid 2> /dev/null` || pids="" - if [ "$pids" != "" ]; - then - echo "Celery is running with pids:" - echo $pids - else - echo "No Celery node processes." - exit 1 - fi -} - -case "$1" in - start) - check_dev_null - check_privsep_dir - start_workers - ;; - - stop) - check_dev_null - stop_workers - ;; - - reload|force-reload) - echo "Use restart" - ;; - - status) - check_status - ;; - - restart) - check_dev_null - check_privsep_dir - restart_workers - ;; - - try-restart) - check_dev_null - check_privsep_dir - restart_workers - ;; - - *) - echo "Usage: /etc/init.d/celeryd {start|stop|restart|try-restart|kill}" - exit 1 - ;; -esac - -exit 0 diff --git a/dbReports/celery/init/DjangoFTP.conf b/dbReports/celery/init/DjangoFTP.conf new file mode 100644 index 00000000..a7a31b7d --- /dev/null +++ b/dbReports/celery/init/DjangoFTP.conf @@ -0,0 +1,15 @@ +# Should be /etc/init/DjangoFTP.conf +description "This service manages the FTP server which authenticates against Django." +author "Brian Bourke-Martin " + +start on runlevel [2345] + +env DJANGO_SETTINGS_MODULE="iondb.settings" +setuid www-data +setgid www-data + +script + chdir /opt/ion/ + sleep 5 + python manage.py ftpserver +end script \ No newline at end of file diff --git a/dbReports/celery/init/celerybeat.conf b/dbReports/celery/init/celerybeat.conf new file mode 100644 index 00000000..8ba9f664 --- /dev/null +++ b/dbReports/celery/init/celerybeat.conf @@ -0,0 +1,39 @@ +# Should be /etc/init/celerybeat.conf +description "This service manages the periodic celery tasks for the Torrent Suite" +author "Brian Bourke-Martin " + +start on (celeryd-running or started celeryd) +stop on (celeryd-stopped or stopping celeryd) + +env DJANGO_SETTINGS_MODULE="iondb.settings" +env CELERY_APP="iondb" +env CELERY_CHDIR="/opt/ion" +env CELERY_LOG_DIR="/var/log/ion" +env CELERY_RUN_DIR="/var/run/celery" +env CELERY_LOG_FILE=celerybeat.log +env CELERY_PID_FILE=celerybeat.pid +env CELERY_LOG_LEVEL="INFO" +env USER=www-data +env GROUP=www-data + +pre-start script + if [ ! -d "CELERY_LOG_DIR" ]; then + mkdir -p "$CELERY_LOG_DIR" + chown "$USER":"$GROUP" "$CELERY_LOG_DIR" + fi + + if [ ! -d "CELERY_RUN_DIR" ]; then + mkdir -p "$CELERY_RUN_DIR" + chown "$USER":"$GROUP" "$CELERY_RUN_DIR" + fi +end script + +exec /usr/bin/celery beat --pidfile="$CELERY_RUN_DIR/$CELERY_PID_FILE" \ + --logfile="$CELERY_LOG_DIR/$CELERY_LOG_FILE" \ + --loglevel="$CELERY_LOG_LEVEL" \ + --app="$CELERY_APP" \ + --schedule=/var/run/celery/celerybeat-schedule \ + --workdir="$CELERY_CHDIR" \ + --uid="$USER" \ + --gid="$GROUP" \ + --no-color \ No newline at end of file diff --git a/dbReports/celery/init/celeryd.conf b/dbReports/celery/init/celeryd.conf new file mode 100644 index 00000000..5b6f5aac --- /dev/null +++ b/dbReports/celery/init/celeryd.conf @@ -0,0 +1,68 @@ +# Should be /etc/init/celeryd.conf +description "This service manages all of the celery queues for the Torrent Suite" +author "Brian Bourke-Martin " + +start on (rabbitmq-server-running or started rabbitmq-server) +stop on (rabbitmq-server-stopped or stopping rabbitmq-server) + +env DJANGO_SETTINGS_MODULE="iondb.settings" +env CELERY_APP="iondb" +env CELERY_CHDIR="/opt/ion" +env CELERY_NODES="w1 plugins periodic slowlane transfer diskutil dmprocess dmmanage" +env CELERY_OPTS="-Ofair --event --time-limit=21600 --queue:w1=w1 --concurrency:w1=4 --queue:plugins=plugins --concurrency:plugins=2 --queue:periodic=periodic --concurrency:periodic=6 --queue:slowlane=slowlane --concurrency:slowlane=1 --queue:transfer=transfer --concurrency:transfer=1 --queue:diskutil=diskutil --concurrency:diskutil=2 --queue:dmprocess=dmprocess --concurrency:dmprocess=8 --queue:dmmanage=dmmanage --concurrency:dmmanage=8" +env CELERY_LOG_DIR="/var/log/ion" +env CELERY_RUN_DIR="/var/run/celery" +env CELERY_LOG_FILE=celery_%n.log +env CELERY_PID_FILE=celery_%n.pid +env CELERY_LOG_LEVEL="INFO" +env USER=www-data +env GROUP=www-data + +script + # we need this section so that pre-stop gets run! + # https://bugs.launchpad.net/upstart/+bug/252996 + while true + do sleep 1d + done +end script + +pre-start script + if [ ! -d "$CELERY_LOG_DIR" ]; then + mkdir -p "$CELERY_LOG_DIR" + chown "$USER":"$GROUP" "$CELERY_LOG_DIR" + fi + + if [ ! -d "$CELERY_RUN_DIR" ]; then + mkdir -p "$CELERY_RUN_DIR" + chown "$USER":"$GROUP" "$CELERY_RUN_DIR" + fi + + /usr/bin/celery multi start $CELERY_NODES \ + --pidfile="$CELERY_RUN_DIR/$CELERY_PID_FILE" \ + --logfile="$CELERY_LOG_DIR/$CELERY_LOG_FILE" \ + --loglevel="$CELERY_LOG_LEVEL" \ + --app="$CELERY_APP" \ + --workdir="$CELERY_CHDIR" \ + --uid=$USER \ + --gid=$GROUP \ + --no-color \ + $CELERY_OPTS +end script + +pre-stop script + /usr/bin/celery multi --verbose stop $CELERY_NODES \ + --pidfile="$CELERY_RUN_DIR/$CELERY_PID_FILE" \ + --logfile="$CELERY_LOG_DIR/$CELERY_LOG_FILE" \ + --loglevel="$CELERY_LOG_LEVEL" \ + --app="$CELERY_APP" \ + --workdir="$CELERY_CHDIR" \ + --uid=$USER \ + --gid=$GROUP \ + --no-color + + # wait for all of the PID's to be removed indicating that the queue's are done + while [ $(ls -1 $CELERY_RUN_DIR/celery_*.pid 2>/dev/null | wc -l) -gt 0 ] + do sleep 1s + done + +end script diff --git a/dbReports/debian/postinst.in b/dbReports/debian/postinst.in index efb1ae52..9d4e8727 100755 --- a/dbReports/debian/postinst.in +++ b/dbReports/debian/postinst.in @@ -13,6 +13,14 @@ unset CELERY_LOADER case "$1" in configure) + KEY_FILE=/var/spool/ion/key + if [ ! -f $KEY_FILE ]; then + cat /dev/urandom | tr -dc 'a-zA-Z0-9' | fold -w 256 | head -n 1 > $KEY_FILE + chown www-data:www-data $KEY_FILE + fi + # updated all instances of the key file to this permission level as per TS-14740 + chmod 0440 $KEY_FILE + # create or update the off-cycle release source url if [ -f /etc/apt/sources.list.d/iontorrent-offcycle.list ]; then sed -i "s/deb http:\/\/ionupdates.com\/updates\/software\/offcycle\/[^0-9.]*\([0-9.]*\).*/deb http:\/\/ionupdates.com\/updates\/software\/offcycle\/@TS_MAJOR@.@TS_MINOR@.@TS_RELEASE@\/ @LSB_RELEASE_CODENAME@\//" /etc/apt/sources.list.d/iontorrent-offcycle.list @@ -63,10 +71,17 @@ case "$1" in chmod -R 777 /results/referenceLibrary/tmap-f3 chmod -R 777 /results/referenceLibrary/disabled chmod -R 777 /results/referenceLibrary/TestFragment + + chown -R www-data:www-data /results/referenceLibrary/TestFragment + #make pluginMedia dir mkdir -p /results/pluginMedia chmod -R 777 /results/pluginMedia + # make the celeryd pid run directory with permissions + mkdir -p /var/run/celery + chmod ugo+rw /var/run/celery + # ------------------------------------------ # Replace existing httpd.conf # ------------------------------------------ @@ -119,16 +134,9 @@ ENDOFAPACHEENV /usr/sbin/a2ensite torrent-server.conf /usr/sbin/a2ensite torrent-server-ssl.conf - # Enable config files - if [ -x /usr/sbin/a2enconf ]; then - # Apache 2.4 on Ubuntu 14.04 - /usr/sbin/a2enconf h5bp-server.conf - /usr/sbin/a2enconf torrent-server-config.conf - else - # Compat with Apache 2.2 and conf.d on Ubuntu 10.04 - /bin/ln -snf /etc/apache2/conf-available/h5bp-server.conf /etc/apache2/conf.d/ - /bin/ln -snf /etc/apache2/conf-available/torrent-server-config.conf /etc/apache2/conf.d/ - fi + # Apache 2.4 on Ubuntu 14.04 + /usr/sbin/a2enconf h5bp-server.conf + /usr/sbin/a2enconf torrent-server-config.conf # apache2 restarted by invoke-rc.d command below @@ -210,6 +218,16 @@ ENDOFAPACHEENV rm -f /results/analysis/output/disabled/tmap-* ln -s -f /results/referenceLibrary/disabled/tmap-* /results/analysis/output/disabled + # ------------------------------------------ + # Create ionian user if it doesn't exist: user is needed to initialize + # ownership of log files below + # ------------------------------------------ + if ! id -u ionian; then + # NOTE: non-unique was added as result of TS-11113 + groupadd ionian --gid=1100 --system + useradd --gid ionian --uid=1100 --non-unique --system ionian + fi + #--- ---# #--- Configure runlevels to start the Ion daemons at boot ---# #--- ---# @@ -217,8 +235,6 @@ ENDOFAPACHEENV ionCrawler ionJobServer ionPlugin - celeryd - celerybeat ) for DAEMON in ${DAEMONS[@]}; do # Need to fix the start/kill priority for the daemons @@ -229,7 +245,6 @@ ENDOFAPACHEENV update-rc.d ${DAEMON} defaults 97 03 done - # ------------------------------------------ # Create directory for logging # ------------------------------------------ @@ -266,8 +281,13 @@ ENDOFAPACHEENV fi invoke-rc.d apache2 stop - invoke-rc.d celerybeat stop - invoke-rc.d celeryd stop + + # we are going to have to do an explicit startup of the postgresql system to make sure it's up and going + # before we attempt to setup the database environment + invoke-rc.d postgresql start + + # doing an explicit wait to make sure that PG is initialized from the previous command + sleep 5 # Setup python and database environment ( @@ -307,6 +327,12 @@ ENDOFAPACHEENV # THIS HAS TO HAPPEN AFTER THE PHP_BASE.HTML FILE IS PUT INTO PLACE DO NOT MOVE /usr/bin/python ./bin/install_script.py + # Load barcode kits to database if needed + echo "Going to add barcode kits if needed..." + /usr/bin/python ./bin/add_barcodes.py "Ion SingleSeq Barcode set 1-24" rundb/fixtures/barcodes/SingleSeq_1-24.csv + /usr/bin/python ./bin/add_barcodes.py "Ion SingleSeq Barcode set 1-96" rundb/fixtures/barcodes/SingleSeq_1-96.csv OVERRIDE + /usr/bin/python ./bin/add_barcodes.py "TagSequencing" rundb/fixtures/barcodes/TagSequencing.csv + # Loads system templates to database if needed echo "Going to add or update system plan templates if needed..." /usr/bin/python ./bin/add_or_update_systemPlanTemplates.py @@ -349,7 +375,7 @@ EOF invoke-rc.d apache2 start invoke-rc.d celeryd start invoke-rc.d celerybeat start - + service DjangoFTP start #load the references for a first time (wget -q -O /dev/null http://localhost/configure/references/ > /dev/null) || \ @@ -366,7 +392,6 @@ EOF # Check whether or not we're online for CSA support upload python -c 'import iondb.bin.djangoinit, iondb.rundb.data.data_export as tasks; tasks.poll_support_site.apply_async(countdown=61)' - #TS-6081: Create DMFileStat objs for existing Results objs without them. echo -n "Migrating to new Data Management..." python -c 'import iondb.bin.djangoinit, iondb.rundb.data.data_management as dm; dm.backfill_create_dmfilestat()' @@ -377,6 +402,9 @@ EOF invoke-rc.d ionPlugin restart invoke-rc.d ionCrawler restart invoke-rc.d avahi-daemon restart + + # fire off the grooming script + /opt/ion/iondb/bin/grooming.py ;; esac diff --git a/dbReports/debian/postrm.in b/dbReports/debian/postrm.in index 348269f9..5ae4a8d8 100755 --- a/dbReports/debian/postrm.in +++ b/dbReports/debian/postrm.in @@ -23,7 +23,8 @@ case "$1" in ionPlugin celeryd celerybeat - ) + DjangoFTP + ) for DAEMON in ${DAEMONS[@]}; do update-rc.d -f ${DAEMON} remove || true done @@ -32,6 +33,7 @@ case "$1" in if [ -h /usr/lib64/libsysfs.so.2.0.2 ]; then rm -f /usr/lib64/libsysfs.so.2.0.2 fi + ;; esac diff --git a/dbReports/debian/preinst.in b/dbReports/debian/preinst.in old mode 100644 new mode 100755 index 0b5fd4b7..5858487e --- a/dbReports/debian/preinst.in +++ b/dbReports/debian/preinst.in @@ -1,28 +1,35 @@ -#!/bin/bash +#!/bin/sh # Copyright (C) 2010 Ion Torrent Systems, Inc. All Rights Reserved set -e -#set -x -echo "preinst $@" + case "$1" in install|upgrade) - if [ -e "/etc/init.d/ionCrawler" ]; then - invoke-rc.d ionCrawler stop - fi - if [ -e "/etc/init.d/ionJobServer" ]; then - invoke-rc.d ionJobServer stop - fi - if [ -e "/etc/init.d/ionPlugin" ]; then - invoke-rc.d ionPlugin stop - fi - - invoke-rc.d apache2 stop - if [ -d @ION_INSTALL_PREFIX@/iondb/ ]; then - find @ION_INSTALL_PREFIX@/iondb/ -name '*.pyc' -delete - fi - - # ionArchive is removed as of this version - make sure sysv links are gone - update-rc.d -f ionArchive remove + + if [ -e "/etc/init.d/ionCrawler" ]; then + invoke-rc.d ionCrawler stop + fi + if [ -e "/etc/init.d/ionJobServer" ]; then + invoke-rc.d ionJobServer stop + fi + if [ -e "/etc/init.d/ionPlugin" ]; then + invoke-rc.d ionPlugin stop + fi + + invoke-rc.d apache2 stop + if [ -d @ION_INSTALL_PREFIX@/iondb/ ]; then + find @ION_INSTALL_PREFIX@/iondb/ -name '*.pyc' -delete + fi + + # ionArchive is removed as of this version - make sure sysv links are gone + update-rc.d -f ionArchive remove + + invoke-rc.d celerybeat stop || true + update-rc.d -f celerybeat remove + + invoke-rc.d celeryd stop || true + update-rc.d -f celeryd remove + + invoke-rc.d DjangoFTP stop || true + update-rc.d -f DjangoFTP remove ;; esac - -exit 0 diff --git a/dbReports/debian/prerm.in b/dbReports/debian/prerm.in index bca586b3..bec8924b 100755 --- a/dbReports/debian/prerm.in +++ b/dbReports/debian/prerm.in @@ -22,6 +22,9 @@ case "$1" in if [ -e "/etc/init.d/celeryd" ]; then invoke-rc.d celeryd stop fi + if [ -e "/etc/init/DjangoFTP" ]; then + invoke-rc.d DjangoFTP stop + fi if [ ! -e @ION_INSTALL_PREFIX@/.computenode ]; then #--- Remove symbolic links ---# diff --git a/dbReports/iondb/anaserve/serve.py b/dbReports/iondb/anaserve/serve.py index 9546edd5..6a78bdc6 100755 --- a/dbReports/iondb/anaserve/serve.py +++ b/dbReports/iondb/anaserve/serve.py @@ -41,36 +41,22 @@ """ import datetime import json -import simplejson import os -import uuid from os import path import re import signal import subprocess import sys import threading -import tempfile import traceback import logging -from logging import handlers as loghandlers +from logging import handlers from twisted.web import xmlrpc, server #for tmap queue -import urllib -import urllib2 -from twisted.internet import utils, reactor -from twisted.web import xmlrpc, server -from twisted.application import service -from twisted.internet import defer, utils -from twisted.python import log -import shutil -from random import choice -import string +from twisted.internet import reactor -#import libs to zip with -import zipfile LOG_FILENAME = '/var/log/ion/jobserver.log' logger = logging.getLogger(__name__) @@ -86,9 +72,14 @@ REFERENCE_LIBRARY_TEMP_DIR = "/results/referenceLibrary/temp/" import iondb.anaserve.djangoinit -import iondb.rundb.models - -__version__ = filter(str.isdigit, "$Revision$") +#from iondb.bin import djangoinit +#from iondb.rundb import models +try: + import iondb.version as version # @UnresolvedImport + GITHASH = version.IonVersionGetGitHash() +except: + GITHASH = "" +__version__ = GITHASH # local settings @@ -450,7 +441,7 @@ def terminate(self): try: logger.debug("terminate job %s, status %s" % (blockjobid, _session.jobStatus(blockjobid))) _session.control(blockjobid, drmaa.JobControlAction.TERMINATE) - except Exception as err: + except Exception: logger.error("Failed to terminate %s" % blockjobid) except: logger.error("DRMAA terminate error reading from %s" % joblistfile) @@ -521,7 +512,7 @@ def suspend(self): return False try: self.proc.send_signal(signal.SIGSTOP) - except Exception as err: + except Exception: logger.warning("SIGSTOP failed") return False return self._running() @@ -532,7 +523,7 @@ def resume(self): return False try: self.proc.send_signal(signal.SIGCONT) - except Exception as err: + except Exception: logger.warning("SIGCONT failed") return False return True @@ -752,7 +743,7 @@ def xmlrpc_updatestatus(self, from ion.reports import uploadMetrics try: uploadMetrics.updateStatus(primarykeyPath, status, reportLink) - except Exception as err: + except Exception: logger.error("Update status failed") return traceback.format_exc() return 0 @@ -893,14 +884,14 @@ def xmlrpc_resultdiskspace(self, pk): update_dmfilestat_diskusage.delay(pk) except: logger.warn("update_diskusage celery task failed to launch") - + # Generate serialized json file for future Data Management Import try: from iondb.rundb.data.tasks import save_serialized_json save_serialized_json.delay(pk) except: logger.warn("save_serialized_json celery task failed") - + return 0 diff --git a/dbReports/iondb/bin/IonMeshDiscoveryManager.py b/dbReports/iondb/bin/IonMeshDiscoveryManager.py index d3ca50fd..621e5beb 100755 --- a/dbReports/iondb/bin/IonMeshDiscoveryManager.py +++ b/dbReports/iondb/bin/IonMeshDiscoveryManager.py @@ -1,18 +1,16 @@ #!/usr/bin/env python -# Copyright (C) 2015 Thermo Fisher Scientific. All Rights Reserved. +# Copyright (C) 2017 Thermo Fisher Scientific. All Rights Reserved. import dbus, gobject, avahi from dbus.mainloop.glib import DBusGMainLoop import threading import time import copy +import socket class IonMeshDiscoveryManager(threading.Thread): - - """ - This class will monitor avahi via dbus in order to detect the current mesh network setup - """ + """This class will monitor avahi via dbus in order to detect the current mesh network setup""" # singleton instance __singleton = None @@ -26,6 +24,9 @@ class IonMeshDiscoveryManager(threading.Thread): # thread lock __threadLock = threading.Lock() + # local host name + __localhost = '' + def __new__(cls, *args, **kwargs): if not cls.__singleton: cls.__singleton = super(IonMeshDiscoveryManager, cls).__new__(cls, *args, **kwargs) @@ -33,8 +34,7 @@ def __new__(cls, *args, **kwargs): # setup dbus stuff cls.__singleton.__bus = dbus.SystemBus(mainloop=DBusGMainLoop()) - cls.__singleton.__server = dbus.Interface(cls.__singleton.__bus.get_object( - avahi.DBUS_NAME, avahi.DBUS_PATH_SERVER), avahi.DBUS_INTERFACE_SERVER) + cls.__singleton.__server = dbus.Interface(cls.__singleton.__bus.get_object(avahi.DBUS_NAME, avahi.DBUS_PATH_SERVER), avahi.DBUS_INTERFACE_SERVER) # Look for self.regtype services and hook into callbacks cls.__singleton.__browser = dbus.Interface(cls.__singleton.__bus.get_object(avahi.DBUS_NAME, cls.__singleton.__server.ServiceBrowserNew( @@ -49,18 +49,21 @@ def __new__(cls, *args, **kwargs): return cls.__singleton + def stop(self): """ Call this to stop the thread as one would expect """ self.__loop.quit() + def run(self): """ Method called with the thread object's "start" method is called """ self.__loop.run() + def getMeshComputers(self): """ This will create a copy of the list of the comupters in the mesh by hostname @@ -72,20 +75,20 @@ def getMeshComputers(self): finally: self.__threadLock.release() + + def getLocalComputer(self): + """Gets the localhost name""" + return self.__localhost or socket.getfqdn() + + def __serviceFound(self, interface, protocol, name, stype, domain, flags): - """ - Callback for when a service needs to be added to the mesh list - :param interface: - :param protocol: - :param name: - :param stype: - :param domain: - :param flags: - """ + """Callback for when a service needs to be added to the mesh list""" # skip local services # http://sources.debian.net/src/avahi/0.6.32-1/avahi-python/avahi/__init__.py/?hl=50#L50 if flags & avahi.LOOKUP_RESULT_LOCAL: + self.__localhost = str(name) return + # add the computer name to the list of mesh computers self.__threadLock.acquire() try: @@ -94,6 +97,7 @@ def __serviceFound(self, interface, protocol, name, stype, domain, flags): finally: self.__threadLock.release() + def __serviceRemoved(self, interface, protocol, name, stype, domain, flags): """ Callback for when a service needs to be removed from the mesh list diff --git a/dbReports/iondb/bin/add_barcodes.py b/dbReports/iondb/bin/add_barcodes.py index 0666e4a7..e0ffa24a 100644 --- a/dbReports/iondb/bin/add_barcodes.py +++ b/dbReports/iondb/bin/add_barcodes.py @@ -13,7 +13,15 @@ import sys -def command_line_add_barcode(name, dest_path): +def _get_new_or_db_barcode(name, index): + if (name and index): + barcodes = dnaBarcode.objects.filter(name = name, index = index) + if barcodes: + print ("db barcode FOUND for name=%s; index=%s" %(name, index)) + return barcodes[0] + return dnaBarcode() + +def command_line_add_barcode(name, dest_path, is_override): """add the barcodes, with CSV validation""" # name = request.POST.get('name', '') @@ -34,14 +42,21 @@ def command_line_add_barcode(name, dest_path): return expectedHeader = ["id_str", "type", "sequence", "floworder", "index", "annotation", "adapter", "score_mode", "score_cutoff"] - if sorted(firstCSV[0]) != sorted(expectedHeader): - print("Barcode csv header is not as expected. Please try again starting with the provided example") - return + ##if sorted(firstCSV[0]) != sorted(expectedHeader): + ## print("Barcode csv header is not as expected. Please try again starting with the provided example") + ## return + # test if the barcode set name has been used before barCodeSet = dnaBarcode.objects.filter(name=name) - if barCodeSet: - print("Error: Barcode set with the same name already exists") - return + if barCodeSet and not is_override: + print("Nothing to do: Barcode set with the same name %s already exists" %name) + return True + + is_override_option = is_override + if is_override and not barCodeSet: + is_override_option = False + + print(">>> Start processing barcode set %s" %name) index = 0 barCodes = [] failed = {} @@ -52,7 +67,9 @@ def command_line_add_barcode(name, dest_path): if invalid: # don't make dna object or add it to the list failed[index] = invalid continue - newBarcode = dnaBarcode() + + newBarcode = _get_new_or_db_barcode(name, index) + newBarcode.name = name # set the name newBarcode.index = index # set index this can be overwritten later nucs = ["sequence", "floworder", "adapter"] # fields that have to be uppercase @@ -102,11 +119,24 @@ def command_line_add_barcode(name, dest_path): if __name__ == "__main__": - if len(sys.argv) != 3: - print("Usage: add_barcode.py NAME BARCODE_CSV_PATH") + if len(sys.argv) < 2 or len(sys.argv) > 4: + print("Usage: add_barcode.py BARCODE_KIT_NAME BARCODE_CSV_PATH or add_barcode.py BARCODE_KIT_NAME BARCODE_CSV_PATH OVERRIDE") sys.exit(1) - name, path = sys.argv[1:3] - if command_line_add_barcode(name, path): + + is_override = False + isDone = False + if len(sys.argv) == 3: + name, path = sys.argv[1:3] + if command_line_add_barcode(name, path, is_override): + isDone = True + elif len(sys.argv) == 4: + name, path, option = sys.argv[1:4] + is_override = (option.lower() == "override") + + if command_line_add_barcode(name, path, is_override): + isDone = True + + if isDone: print("Completed successfully") sys.exit(0) else: diff --git a/dbReports/iondb/bin/add_or_update_systemPlanTemplates.py b/dbReports/iondb/bin/add_or_update_systemPlanTemplates.py old mode 100755 new mode 100644 index 67079117..6b9d7072 --- a/dbReports/iondb/bin/add_or_update_systemPlanTemplates.py +++ b/dbReports/iondb/bin/add_or_update_systemPlanTemplates.py @@ -313,7 +313,7 @@ def finish_sys_template(sysTemplate, isCreated, templateParams, plugins={}): eas.reference = templateParams.reference hasChanges = True - if (eas.selectedPlugins != plugins): + if not simple_compare_dict(eas.selectedPlugins, plugins): print ">>> DIFF: orig eas.selectedPlugins=%s for system template.id=%d; name=%s" % \ (eas.selectedPlugins, sysTemplate.id, sysTemplate.planName) print ">>> DIFF: NEW selectedPlugins=%s for system template.id=%d; name=%s" % \ @@ -368,18 +368,45 @@ def finish_sys_template(sysTemplate, isCreated, templateParams, plugins={}): return sysTemplate -def _get_third_party_plugin_dict(pluginId=9999, pluginName=""): - pluginUserInput = {} +def _get_plugin_dict(pluginName, userInput={}): + try: + selectedPlugin = models.Plugin.objects.get(name=pluginName, selected=True, active=True) + pluginDict = { + "id": selectedPlugin.id, + "name": selectedPlugin.name, + "version": selectedPlugin.version, + "userInput": userInput, + "features": [] + } + except models.Plugin.DoesNotExist: + pluginDict = { + "id": 9999, + "name": pluginName, + "version": "1.0", + "userInput": userInput, + "features": [] + } - thirdPartyPluginDict = { - "id": pluginId, - "name": pluginName, - "version": "1.0", - "userInput": pluginUserInput, - "features": [] - } - - return thirdPartyPluginDict + return pluginDict + + +def simple_compare_dict(dict1, dict2): + ''' accepts multi-level dictionaries + compares values as strings, will not report type mismatch + ''' + if sorted(dict1.keys()) != sorted(dict2.keys()): + return False + + for key, value in dict1.iteritems(): + if isinstance(value, dict): + if not simple_compare_dict(value, dict2[key]): + return False + elif isinstance(value, list): + if sorted(value) != sorted(dict2[key]): + return False + elif str(value) != str(dict2[key]): + return False + return True def add_or_update_sys_template(templateParams, isSystemDefault=False): @@ -446,14 +473,13 @@ def add_or_update_sys_template(templateParams, isSystemDefault=False): sysTemplate.templatingSize = templateParams.templatingSize hasChanges = True - if templateParams.libraryReadLength: - if (sysTemplate.libraryReadLength != templateParams.libraryReadLength): - print ">>> DIFF: orig sysTemplate.libraryReadLength=%s for system template.id=%d; name=%s" % \ - (sysTemplate.libraryReadLength, - sysTemplate.id, sysTemplate.planName) + if (sysTemplate.libraryReadLength != templateParams.libraryReadLength): + print ">>> DIFF: orig sysTemplate.libraryReadLength=%s for system template.id=%d; name=%s" % \ + (sysTemplate.libraryReadLength, + sysTemplate.id, sysTemplate.planName) - sysTemplate.libraryReadLength = templateParams.libraryReadLength - hasChanges = True + sysTemplate.libraryReadLength = templateParams.libraryReadLength + hasChanges = True if (sysTemplate.planStatus not in ["planned", "inactive"]): print ">>> DIFF: orig sysTemplate.planStatus=%s not supported for system template.id=%d; name=%s" % \ @@ -576,28 +602,21 @@ def add_or_update_default_system_templates(): def add_or_update_ampliseq_system_templates(): # ampliseq + CATEGORIES = "onco_solidTumor" # 3 templateParams = TemplateParams("Ion AmpliSeq Cancer Hotspot Panel v2", PGM, "AMPS") - templateParams.update({ - "flows": 500, - "libraryKitName": "Ion AmpliSeq 2.0 Library Kit", - "reference": "hg19" - }) - sysTemplate, isCreated, isUpdated = add_or_update_sys_template(templateParams) - finish_sys_template(sysTemplate, isCreated, templateParams) - - # 4 - templateParams = TemplateParams("Ion AmpliSeq Cancer Panel", PGM, "AMPS") templateParams.update({ "flows": 500, "libraryKitName": "Ion AmpliSeq 2.0 Library Kit", "reference": "hg19", - "targetRegionBedFile": "/hg19/unmerged/detail/HSMv12.1_reqions_NO_JAK2_NODUP.bed", - "hotSpotRegionBedFile":"/hg19/unmerged/detail/HSMv12.1_hotspots_NO_JAK2.bed" + "categories": CATEGORIES }) sysTemplate, isCreated, isUpdated = add_or_update_sys_template(templateParams) finish_sys_template(sysTemplate, isCreated, templateParams) + # 4 - retired + # templateParams = TemplateParams("Ion AmpliSeq Cancer Panel", PGM, "AMPS") + # 5 templateParams = TemplateParams("Ion AmpliSeq Cancer Panel 1_0 Lib Chem", PGM, "AMPS") templateParams.update({ @@ -616,6 +635,7 @@ def add_or_update_ampliseq_system_templates(): "libraryKitName": "Ion AmpliSeq 2.0 Library Kit", "reference": "hg19", "targetRegionBedFile": "/hg19/unmerged/detail/4477685_CCP_bedfile_20120517.bed", + "categories": CATEGORIES }) sysTemplate, isCreated, isUpdated = add_or_update_sys_template(templateParams) finish_sys_template(sysTemplate, isCreated, templateParams) @@ -642,10 +662,11 @@ def add_or_update_ampliseq_system_templates(): # 9 templateParams = TemplateParams("Ion AmpliSeq Inherited Disease Panel", PGM, "AMPS") templateParams.update({ - "chipType": "316", + "chipType": "318", "flows": 500, "libraryKitName": "Ion AmpliSeq 2.0 Library Kit", "reference": "hg19", + "categories": "inheritedDisease", "targetRegionBedFile": "/hg19/unmerged/detail/4477686_IDP_bedfile_20120613.bed", }) sysTemplate, isCreated, isUpdated = add_or_update_sys_template(templateParams) @@ -668,25 +689,10 @@ def add_or_update_ampliseq_rna_system_templates(): # pre-select plugins plugins = {} - pluginUserInput = {} - - selectedPlugins = models.Plugin.objects.filter( - name__in=["coverageAnalysis"], selected=True, active=True) - - for selectedPlugin in selectedPlugins: - - pluginDict = { - "id": selectedPlugin.id, - "name": selectedPlugin.name, - "version": selectedPlugin.version, - "userInput": pluginUserInput, - "features": [] - } - - plugins[selectedPlugin.name] = pluginDict + plugins["coverageAnalysis"] = _get_plugin_dict("coverageAnalysis") thirdPartyPluginName = "PartekFlowUploader" - plugins[thirdPartyPluginName] = _get_third_party_plugin_dict(pluginId=9999, pluginName=thirdPartyPluginName) + plugins[thirdPartyPluginName] = _get_plugin_dict(thirdPartyPluginName) finish_sys_template(sysTemplate, isCreated, templateParams, plugins) @@ -790,7 +796,7 @@ def add_or_update_rna_system_templates(): plugins = {} thirdPartyPluginName = "PartekFlowUploader" - plugins[thirdPartyPluginName] = _get_third_party_plugin_dict(pluginId=9999, pluginName=thirdPartyPluginName) + plugins[thirdPartyPluginName] = _get_plugin_dict(thirdPartyPluginName) finish_sys_template(sysTemplate, isCreated, templateParams, plugins) @@ -807,25 +813,10 @@ def add_or_update_rna_system_templates(): # pre-select plugins plugins = {} - pluginUserInput = {} - - selectedPlugins = models.Plugin.objects.filter( - name__in=["RNASeqAnalysis"], selected=True, active=True).order_by("-id") - if len(selectedPlugins) > 0: - selectedPlugin = selectedPlugins[0] - - pluginDict = { - "id": selectedPlugin.id, - "name": selectedPlugin.name, - "version": selectedPlugin.version, - "userInput": pluginUserInput, - "features": [] - } - - plugins[selectedPlugin.name] = pluginDict + plugins["RNASeqAnalysis"] = _get_plugin_dict("RNASeqAnalysis") thirdPartyPluginName = "PartekFlowUploader" - plugins[thirdPartyPluginName] = _get_third_party_plugin_dict(pluginId=9999, pluginName=thirdPartyPluginName) + plugins[thirdPartyPluginName] = _get_plugin_dict(thirdPartyPluginName) finish_sys_template(sysTemplate, isCreated, templateParams, plugins) @@ -864,7 +855,8 @@ def add_or_update_metagenomics_system_templates(): "templatingKitName": "Ion PGM Template OT2 400 Kit", "sampleGrouping": "Self", "sequencekitname": "IonPGM400Kit", - "libraryKitName": "IonPlusFragmentLibKit" + "libraryKitName": "IonPlusFragmentLibKit", + "categories": "16s" }) sysTemplate, isCreated, isUpdated = add_or_update_sys_template(templateParams) finish_sys_template(sysTemplate, isCreated, templateParams) @@ -872,6 +864,7 @@ def add_or_update_metagenomics_system_templates(): def add_or_update_oncomine_system_templates(): # Oncomine + CATEGORIES = "Oncomine;onco_solidTumor" # 22 templateParams = TemplateParams("Oncomine Comprehensive DNA", PGM, "AMPS") templateParams.update({ @@ -879,7 +872,7 @@ def add_or_update_oncomine_system_templates(): "flows": 400, "controlSequencekitname": "Ion AmpliSeq Sample ID Panel", "sampleGrouping": "Self", - "categories": "Oncomine", + "categories": CATEGORIES, "libraryKitName": "Ion AmpliSeq 2.0 Library Kit", "barcodeKitName": "IonXpress", "reference": "hg19", @@ -895,7 +888,7 @@ def add_or_update_oncomine_system_templates(): "flows": 400, "controlSequencekitname": "Ion AmpliSeq Sample ID Panel", "sampleGrouping": "Self", - "categories": "Oncomine", + "categories": CATEGORIES, "libraryKitName": "Ion AmpliSeq RNA Library Kit", "barcodeKitName": "IonXpress", }) @@ -910,38 +903,22 @@ def add_or_update_oncomine_system_templates(): "flows": 400, "controlSequencekitname": "Ion AmpliSeq Sample ID Panel", "sampleGrouping": "DNA and Fusions", - "categories": "Oncomine", + "categories": CATEGORIES, "libraryKitName": "Ion AmpliSeq 2.0 Library Kit", "barcodeKitName": "IonXpress", "reference": "hg19", - "targetRegionBedFile": "/hg19/unmerged/detail/OCP3.20140718.designed.bed", - "hotSpotRegionBedFile":"/hg19/unmerged/detail/OCP3.20140611.hotspots.blist.bed" }) sysTemplate, isCreated, isUpdated = add_or_update_sys_template(templateParams) # pre-select plugins plugins = {} - pluginUserInput = {} - - selectedPlugins = models.Plugin.objects.filter( - name__in=["coverageAnalysis"], selected=True, active=True).order_by("-id") - if len(selectedPlugins) > 0: - selectedPlugin = selectedPlugins[0] - - pluginDict = { - "id": selectedPlugin.id, - "name": selectedPlugin.name, - "version": selectedPlugin.version, - "userInput": pluginUserInput, - "features": [] - } - - plugins[selectedPlugin.name] = pluginDict + plugins["coverageAnalysis"] = _get_plugin_dict("coverageAnalysis") finish_sys_template(sysTemplate, isCreated, templateParams, plugins) def add_or_update_onconet_system_templates(): # OncoNetwork + CATEGORIES = "Onconet;onco_solidTumor" # 25 templateParams = TemplateParams("Ion AmpliSeq Colon and Lung Cancer Panel v2", PGM, "AMPS") templateParams.update({ @@ -949,7 +926,7 @@ def add_or_update_onconet_system_templates(): "flows": 400, "controlSequencekitname": "Ion AmpliSeq Sample ID Panel", "sampleGrouping": "Self", - "categories": "Onconet", + "categories": CATEGORIES, "libraryKitName": "Ion AmpliSeq 2.0 Library Kit", "barcodeKitName": "IonXpress", "reference": "hg19", @@ -965,7 +942,7 @@ def add_or_update_onconet_system_templates(): "flows": 400, "controlSequencekitname": "Ion AmpliSeq Sample ID Panel", "sampleGrouping": "Self", - "categories": "Onconet", + "categories": CATEGORIES, "libraryKitName": "Ion AmpliSeq RNA Library Kit", "barcodeKitName": "IonXpress", }) @@ -980,7 +957,7 @@ def add_or_update_onconet_system_templates(): "flows": 400, "controlSequencekitname": "Ion AmpliSeq Sample ID Panel", "sampleGrouping": "DNA and Fusions", - "categories": "Onconet", + "categories": CATEGORIES, "libraryKitName": "Ion AmpliSeq 2.0 Library Kit", "barcodeKitName": "IonXpress", "reference": "hg19" @@ -1004,37 +981,13 @@ def add_or_update_onconet_system_templates(): # pre-select plugins plugins = {} - pluginUserInput = {} - - selectedPlugins = models.Plugin.objects.filter( - name__in=["ampliSeqRNA"], selected=True, active=True) - - for selectedPlugin in selectedPlugins: - pluginDict = { - "id": selectedPlugin.id, - "name": selectedPlugin.name, - "version": selectedPlugin.version, - "userInput": pluginUserInput, - "features": [] - } - - plugins[selectedPlugin.name] = pluginDict - - if len(selectedPlugins) < 1: - ionCommunityPluginDict = { - "id": 9999, - "name": "ampliSeqRNA", - "version": "1.0", - "userInput": pluginUserInput, - "features": [] - } - - plugins["ampliSeqRNA"] = ionCommunityPluginDict + plugins["ampliSeqRNA"] = _get_plugin_dict("ampliSeqRNA") finish_sys_template(sysTemplate, isCreated, templateParams, plugins) def add_or_update_ocp_focus_system_templates(): + CATEGORIES = "Oncomine;onco_solidTumor" # OCP Focus OCP_FOCUS_SEQ_KIT_NAME = "IonPGMSelectKit" OCP_FOCUS_LIB_KIT_NAME = "Ion PGM Select Library Kit" @@ -1047,7 +1000,7 @@ def add_or_update_ocp_focus_system_templates(): "flows": 400, "templatingKitName": OCP_FOCUS_TEMPLATE_KIT_NAME, "sampleGrouping": "Self", - "categories": "Oncomine", + "categories": CATEGORIES, "sequencekitname": OCP_FOCUS_SEQ_KIT_NAME, "libraryKitName": OCP_FOCUS_LIB_KIT_NAME, "barcodeKitName": OCP_FOCUS_BARCODE_KIT_NAME, @@ -1064,7 +1017,7 @@ def add_or_update_ocp_focus_system_templates(): "flows": 400, "templatingKitName": OCP_FOCUS_TEMPLATE_KIT_NAME, "sampleGrouping": "Self", - "categories": "Oncomine", + "categories": CATEGORIES, "sequencekitname": OCP_FOCUS_SEQ_KIT_NAME, "libraryKitName": OCP_FOCUS_LIB_KIT_NAME, "barcodeKitName": OCP_FOCUS_BARCODE_KIT_NAME @@ -1080,7 +1033,7 @@ def add_or_update_ocp_focus_system_templates(): "flows": 400, "templatingKitName": OCP_FOCUS_TEMPLATE_KIT_NAME, "sampleGrouping": "DNA and Fusions", - "categories": "Oncomine", + "categories": CATEGORIES, "sequencekitname": OCP_FOCUS_SEQ_KIT_NAME, "libraryKitName": OCP_FOCUS_LIB_KIT_NAME, "barcodeKitName": OCP_FOCUS_BARCODE_KIT_NAME, @@ -1093,38 +1046,78 @@ def add_or_update_ocp_focus_system_templates(): def add_or_update_reproseq_system_templates(): # ReproSeq DEFAULT_P1_3_PRIME_ADAPTER_SEQUENCE = "ATCACCGACTGCCCATAGAGAGGAAAGCGG" + BARCODE_KIT_PGM = "Ion SingleSeq Barcode set 1-24" + BARCODE_KIT_S5 = "Ion SingleSeq Barcode set 1-96" + CATEGORIES = "repro" + LIBRARY_KIT = "IonPicoPlex" + LIBRARY_READ_LENGTH = 0 + REFERENCE = "hg19" + RUN_TYPE = "WGNM" + + # pre-select plugins + plugins = {} + plugins["FilterDuplicates"] = _get_plugin_dict("FilterDuplicates") + # 32 - templateParams = TemplateParams("Ion ReproSeq Aneuploidy", PGM, "WGNM") + templateParams = TemplateParams("Ion ReproSeq Aneuploidy - Ion PGM System", PGM, RUN_TYPE) templateParams.update({ "chipType": "318", "flows": 250, "templatingKitName": "Ion PGM Template IA Tech Access Kit", "sampleGrouping": "Self", "sequencekitname": "IonPGMHiQ", - "libraryKitName": "IonPicoPlex", - "barcodeKitName": "Ion SingleSeq Barcode set 1", - "threePrimeAdapter": DEFAULT_P1_3_PRIME_ADAPTER_SEQUENCE + "libraryKitName": LIBRARY_KIT, + "barcodeKitName": BARCODE_KIT_PGM, + "reference": REFERENCE, + "threePrimeAdapter": DEFAULT_P1_3_PRIME_ADAPTER_SEQUENCE, + "categories": CATEGORIES }) sysTemplate, isCreated, isUpdated = add_or_update_sys_template(templateParams) - finish_sys_template(sysTemplate, isCreated, templateParams) + finish_sys_template(sysTemplate, isCreated, templateParams, plugins) + #TODO- need finalized barcodeKit # 33 is S5 System Default Template + flowOrderList = models.FlowOrder.objects.filter(name="Ion samba.contradanza") + PGS_S5_FLOWORDER = flowOrderList[0].flowOrder if flowOrderList else "" + + threePrimeAdapterList = models.ThreePrimeadapter.objects.filter(name = "Ion P1B") + PGS_S5_3PrimeAdapter = threePrimeAdapterList[0].sequence if threePrimeAdapterList else "" + + templateParams = TemplateParams("Ion ReproSeq Aneuploidy - Ion S5 System", S5, RUN_TYPE) + templateParams.update({ + "chipType": "530", + "flows": 250, + "flowOrder" : PGS_S5_FLOWORDER, + "templatingKitName": "Ion Chef PGS V1", + "sampleGrouping": "Self", + "sequencekitname": "Ion S5 ExT Sequencing Kit", + "libraryKitName": LIBRARY_KIT, + "libraryReadLength" : LIBRARY_READ_LENGTH, + "barcodeKitName": BARCODE_KIT_S5, + "reference": REFERENCE, + "threePrimeAdapter": PGS_S5_3PrimeAdapter, + "categories": CATEGORIES + }) + sysTemplate, isCreated, isUpdated = add_or_update_sys_template(templateParams) + finish_sys_template(sysTemplate, isCreated, templateParams, plugins) def add_or_update_tagseq_system_templates(): - LIQUID_BIOPSY_PLUGIN_CONFIG_FILENAME = "rundb/fixtures/systemtemplateparams/tagseq_cfdna_lowstringency_parameters.json" - TUMOR_PLUGIN_CONFIG_FILENAME = "rundb/fixtures/systemtemplateparams/tagseq_ffpe_lowstringency_parameters.json" + LIQUID_BIOPSY_PLUGIN_CONFIG_FILENAME = "rundb/fixtures/systemtemplateparams/tagseq_liquidbiopsy_parameters.json" + TUMOR_PLUGIN_CONFIG_FILENAME = "rundb/fixtures/systemtemplateparams/tagseq_tumor_parameters.json" # 34 Lung Liquid Biopsy DNA sysTemplate, isCreated, isUpdated, templateParams = add_or_update_tagseq_system_template("Oncomine Lung Liquid Biopsy DNA") liquid_biopsy_plugins = create_tagseq_plugins(LIQUID_BIOPSY_PLUGIN_CONFIG_FILENAME) + templateParams.selectedPlugins = liquid_biopsy_plugins finish_sys_template(sysTemplate, isCreated, templateParams, liquid_biopsy_plugins) # 35 Lung Tumor DNA sysTemplate, isCreated, isUpdated, templateParams = add_or_update_tagseq_system_template("Oncomine Lung Tumor DNA") tumor_plugins = create_tagseq_plugins(TUMOR_PLUGIN_CONFIG_FILENAME) + templateParams.selectedPlugins = tumor_plugins finish_sys_template(sysTemplate, isCreated, templateParams, tumor_plugins) @@ -1152,7 +1145,7 @@ def add_or_update_tagseq_system_templates(): def add_or_update_tagseq_system_template(templateName): # Tag Sequencing TAG_SEQ_APPLICATION_GROUP = "onco_liquidBiopsy" - TAG_SEQ_BARCODE_KIT_NAME = "IonCode - TagSequencing" + TAG_SEQ_BARCODE_KIT_NAME = "TagSequencing" TAG_SEQ_CATEGORIES = "barcodes_8" TAG_SEQ_CHIP_NAME = "530" TAG_SEQ_FLOWS = 500 @@ -1193,19 +1186,7 @@ def create_tagseq_plugins(configFileName = None): if data: pluginUserInput = data - selectedPlugins = models.Plugin.objects.filter( - name__in=["variantCaller"], selected=True, active=True) - - for selectedPlugin in selectedPlugins: - pluginDict = { - "id": selectedPlugin.id, - "name": selectedPlugin.name, - "version": selectedPlugin.version, - "userInput": pluginUserInput, - "features": [] - } - - plugins[selectedPlugin.name] = pluginDict + plugins["variantCaller"] = _get_plugin_dict("variantCaller", pluginUserInput) return plugins @@ -1213,7 +1194,7 @@ def create_tagseq_plugins(configFileName = None): def add_or_update_oncomine_ocav1_system_templates(): # OCAv1 OCAV1_BARCODE_KIT_NAME = "IonXpress" - OCAV1_CATEGORIES = "Oncomine" + OCAV1_CATEGORIES = "Oncomine;onco_solidTumor" OCAV1_CHIP_NAME = "540" OCAV1_FLOWS = 400 OCAV1_LIB_KIT_NAME = "Ion AmpliSeq 2.0 Library Kit" @@ -1262,7 +1243,7 @@ def add_or_update_oncomine_ocav1_system_templates(): def add_or_update_oncomine_ocav2_system_templates(): # OCAv2 OCAV2_BARCODE_KIT_NAME = "IonXpress" - OCAV2_CATEGORIES = "Oncomine;ocav2" + OCAV2_CATEGORIES = "Oncomine;ocav2;onco_solidTumor" OCAV2_CHIP_NAME = "540" OCAV2_FLOWS = 400 OCAV2_LIB_KIT_NAME = "Ion AmpliSeq 2.0 Library Kit" @@ -1273,23 +1254,8 @@ def add_or_update_oncomine_ocav2_system_templates(): # pre-select plugins plugins = {} - pluginUserInput = {} - - selectedPlugins = models.Plugin.objects.filter( - name__in=["coverageAnalysis"], selected=True, active=True) - - for selectedPlugin in selectedPlugins: - - pluginDict = { - "id": selectedPlugin.id, - "name": selectedPlugin.name, - "version": selectedPlugin.version, - "userInput": pluginUserInput, - "features": [] - } - - plugins[selectedPlugin.name] = pluginDict - + plugins["coverageAnalysis"] = _get_plugin_dict("coverageAnalysis") + # 42 Oncomine Comprehensive v2 DNA and Fusions for S5 templateParams = TemplateParams("Oncomine Comprehensive v2 DNA and Fusions for S5", S5, "AMPS_DNA_RNA") templateParams.update({ @@ -1312,7 +1278,8 @@ def add_or_update_oncomine_ocav2_system_templates(): def add_or_update_oncomine_ocav3_system_templates(): # OCAv3 OCAV3_BARCODE_KIT_NAME = "IonXpress" - OCAV3_CATEGORIES = "Oncomine" + OCAV3_CATEGORIES = "Oncomine;onco_solidTumor" + OCAV3_CATEGORIES_2 = "Oncomine;barcodes_16;onco_solidTumor" OCAV3_CHIP_NAME = "540" OCAV3_FLOWS = 400 OCAV3_FUSION_LIB_KIT_NAME = "Ion AmpliSeq Library Kit Plus" @@ -1321,26 +1288,11 @@ def add_or_update_oncomine_ocav3_system_templates(): OCAV3_REFERENCE = "hg19" OCAV3_SEQ_KIT_NAME = "Ion S5 Sequencing Kit" OCAV3_TEMPLATE_KIT_NAME = "Ion Chef S540 V1" - OCAV3_STATUS = "inactive" + OCAV3_STATUS = "planned" # pre-select plugins plugins = {} - pluginUserInput = {} - - selectedPlugins = models.Plugin.objects.filter( - name__in=["coverageAnalysis"], selected=True, active=True) - - for selectedPlugin in selectedPlugins: - - pluginDict = { - "id": selectedPlugin.id, - "name": selectedPlugin.name, - "version": selectedPlugin.version, - "userInput": pluginUserInput, - "features": [] - } - - plugins[selectedPlugin.name] = pluginDict + plugins["coverageAnalysis"] = _get_plugin_dict("coverageAnalysis") # 43 Oncomine Comprehensive v3 DNA templateParams = TemplateParams("Oncomine Comprehensive v3 DNA", S5, "AMPS") @@ -1361,12 +1313,12 @@ def add_or_update_oncomine_ocav3_system_templates(): sysTemplate, isCreated, isUpdated = add_or_update_sys_template(templateParams) finish_sys_template(sysTemplate, isCreated, templateParams, plugins) - # 44 Oncomine Comprehensive v3 DNA and Fusion ST - templateParams = TemplateParams("Oncomine Comprehensive v3 DNA and Fusion ST", S5, "AMPS_DNA_RNA") + # 44 Oncomine Comprehensive v3 DNA and Fusions + templateParams = TemplateParams("Oncomine Comprehensive v3 DNA and Fusions", S5, "AMPS_DNA_RNA") templateParams.update({ "applicationGroup": "DNA + RNA", "barcodeKitName": OCAV3_BARCODE_KIT_NAME, - "categories": OCAV3_CATEGORIES, + "categories": OCAV3_CATEGORIES_2, "chipType": OCAV3_CHIP_NAME, "flows": OCAV3_FLOWS, "libraryKitName": OCAV3_LIB_KIT_NAME, @@ -1380,8 +1332,8 @@ def add_or_update_oncomine_ocav3_system_templates(): sysTemplate, isCreated, isUpdated = add_or_update_sys_template(templateParams) finish_sys_template(sysTemplate, isCreated, templateParams, plugins) - # 45 Oncomine Fusion ST - templateParams = TemplateParams("Oncomine Fusion ST", S5, "AMPS_RNA") + # 45 Oncomine Comprehensive v3 Fusions + templateParams = TemplateParams("Oncomine Comprehensive v3 Fusions", S5, "AMPS_RNA") templateParams.update({ "applicationGroup": "DNA + RNA", "barcodeKitName": OCAV3_BARCODE_KIT_NAME, @@ -1403,7 +1355,7 @@ def add_or_update_oncomine_ocav3_system_templates(): def add_or_update_oncomine_pediatric_system_templates(): # pediatric BARCODE_KIT_NAME = "IonXpress" - CATEGORIES = "Oncomine" + CATEGORIES = "onco_solidTumor;onco_heme" CHIP_NAME = "540" FLOWS = 400 FUSION_LIB_KIT_NAME = "Ion AmpliSeq RNA Library Kit" @@ -1474,19 +1426,19 @@ def add_or_update_oncomine_pediatric_system_templates(): def add_or_update_oncomine_BRCA_system_templates(): # BRCA - BARCODE_KIT_NAME = "IonCode Barcodes 1-32" - CATEGORIES = "Oncomine" + BARCODE_KIT_NAME = "IonXpress" + CATEGORIES = "Oncomine;onco_solidTumor;inheritedDisease" CHIP_NAME_PGM = "318" CHIP_NAME_S5 = "530" FLOWS = 500 - LIB_KIT_NAME = "Ampliseq DNA V1" + LIB_KIT_NAME = "Ion AmpliSeq Library Kit Plus" LIBRARY_READ_LENGTH = 200 REFERENCE = "hg19" SEQ_KIT_NAME_PGM = "IonPGMHiQ" SEQ_KIT_NAME_S5 = "Ion S5 Sequencing Kit" TEMPLATE_KIT_NAME_PGM = "Ion PGM Hi-Q View Chef Kit" TEMPLATE_KIT_NAME_S5 = "Ion Chef S530 V1" - + # 49 Oncomine BRCA for PGM templateParams = TemplateParams("Oncomine BRCA Research for PGM", PGM, "AMPS") templateParams.update({ @@ -1525,18 +1477,24 @@ def add_or_update_oncomine_BRCA_system_templates(): def add_or_update_immune_response_system_templates(): - PLAN_STATUS = "inactive" - - # 51 Immune Response Panel - templateParams = TemplateParams("Immune Response Panel", S5, "AMPS_RNA") + PLAN_STATUS = "planned" + CATEGORIES = "onco_immune" + PLUGIN = "immuneResponseRNA" + REFERENCE = "ImmuneResponse_v3.1" + BEDFILE = "/%s/unmerged/detail/ImmuneResponse_v3.1_target_designed_20160908.bed" % REFERENCE + + # 51 Immune Response Panel S5 + templateParams = TemplateParams("Oncomine Immune Response Research Assay for S5 with Chef", S5, "AMPS_RNA") templateParams.update({ "applicationGroup": "RNA", - "barcodeKitName": "IonXpress", + "barcodeKitName": "IonCode Barcodes 1-32", + "categories": CATEGORIES, "chipType": "530", "flows": 500, - "libraryKitName": "Ion AmpliSeq 2.0 Library Kit", + "libraryKitName": "Ampliseq DNA V1", "libraryReadLength" : 200, - "reference": "hg19_rna_ImmuneResponsePanelv2", + "reference": REFERENCE, + "targetRegionBedFile": BEDFILE, "sampleGrouping": "Self", "sequencekitname": "Ion S5 Sequencing Kit", "templatingKitName": "Ion Chef S530 V1", @@ -1547,30 +1505,32 @@ def add_or_update_immune_response_system_templates(): # pre-select plugins plugins = {} pluginUserInput = {} + plugins[PLUGIN] = _get_plugin_dict(PLUGIN, pluginUserInput) - pluginName = "immuneResponseRNA" - selectedPlugins = models.Plugin.objects.filter( - name__in=[pluginName], selected=True, active=True).order_by("-id") - if len(selectedPlugins) > 0: - selectedPlugin = selectedPlugins[0] - - pluginDict = { - "id": selectedPlugin.id, - "name": selectedPlugin.name, - "version": selectedPlugin.version, - "userInput": pluginUserInput, - "features": [] - } - - plugins[selectedPlugin.name] = pluginDict - else: - plugins[pluginName] = _get_third_party_plugin_dict(pluginId=9999, pluginName=pluginName) + finish_sys_template(sysTemplate, isCreated, templateParams, plugins) + #67 Immune Response Panel PGM + templateParams = TemplateParams("Oncomine Immune Response Research Assay for PGM with OT2", PGM, "AMPS_RNA") + templateParams.update({ + "applicationGroup": "RNA", + "barcodeKitName": "IonXpress", + "categories": CATEGORIES, + "chipType": "318", + "flows": 500, + "libraryKitName": "Ion AmpliSeq 2.0 Library Kit", + "reference": REFERENCE, + "targetRegionBedFile": BEDFILE, + "sequencekitname": "IonPGMHiQView", + "templatingKitName": "Ion PGM Hi-Q View OT2 Kit - 200", + "planStatus" : PLAN_STATUS + }) + sysTemplate, isCreated, isUpdated = add_or_update_sys_template(templateParams) finish_sys_template(sysTemplate, isCreated, templateParams, plugins) def add_or_update_S5_ocp_focus_system_templates(): # OCP Focus + OCP_S5_FOCUS_CATEGORIES = "Oncomine;onco_solidTumor" OCP_S5_FOCUS_CHIP = "520" OCP_S5_FOCUS_FLOWS = 400 OCP_S5_FOCUS_LIBRARY_KIT_NAME = "Ion AmpliSeq 2.0 Library Kit" @@ -1581,11 +1541,11 @@ def add_or_update_S5_ocp_focus_system_templates(): templateParams = TemplateParams("Oncomine Focus DNA for S5", S5, "AMPS") templateParams.update({ "chipType": OCP_S5_FOCUS_CHIP, + "categories": OCP_S5_FOCUS_CATEGORIES, "flows": OCP_S5_FOCUS_FLOWS, "libraryKitName": OCP_S5_FOCUS_LIBRARY_KIT_NAME, "templatingKitName": OCP_S5_FOCUS_TEMPLATE_KIT_NAME, "sampleGrouping": "Self", - "categories": "Oncomine", "barcodeKitName": OCP_S5_FOCUS_BARCODE_KIT_NAME, "reference": "hg19" }) @@ -1596,12 +1556,13 @@ def add_or_update_S5_ocp_focus_system_templates(): templateParams = TemplateParams("Oncomine Focus Fusions for S5", S5, "AMPS_RNA") templateParams.update({ "applicationGroup": "DNA + RNA", + "categories": OCP_S5_FOCUS_CATEGORIES, "chipType": OCP_S5_FOCUS_CHIP, "flows": OCP_S5_FOCUS_FLOWS, "libraryKitName": OCP_S5_FOCUS_LIBRARY_KIT_NAME, "templatingKitName": OCP_S5_FOCUS_TEMPLATE_KIT_NAME, "sampleGrouping": "Self", - "categories": "Oncomine", + "categories": OCP_S5_FOCUS_CATEGORIES, "barcodeKitName": OCP_S5_FOCUS_BARCODE_KIT_NAME }) sysTemplate, isCreated, isUpdated = add_or_update_sys_template(templateParams) @@ -1611,12 +1572,12 @@ def add_or_update_S5_ocp_focus_system_templates(): templateParams = TemplateParams("Oncomine Focus DNA and Fusions for S5", S5, "AMPS_DNA_RNA") templateParams.update({ "applicationGroup": "DNA + RNA", + "categories": OCP_S5_FOCUS_CATEGORIES, "chipType": OCP_S5_FOCUS_CHIP, "flows": OCP_S5_FOCUS_FLOWS, "libraryKitName": OCP_S5_FOCUS_LIBRARY_KIT_NAME, "templatingKitName": OCP_S5_FOCUS_TEMPLATE_KIT_NAME, "sampleGrouping": "DNA and Fusions", - "categories": "Oncomine", "barcodeKitName": OCP_S5_FOCUS_BARCODE_KIT_NAME, "reference": "hg19" }) @@ -1846,9 +1807,9 @@ def add_or_update_hid_system_templates(): def add_or_update_hid_dexter_system_templates(): # HID - default_flowOrderList = models.FlowOrder.objects.filter(name="Ion samba.gafieira") + default_flowOrderList = models.FlowOrder.objects.filter(name="Ion samba HID2") - BARCODE_KIT_NAME = "IonCode Barcodes 1-32" + BARCODE_KIT_NAME = "IonCode" CATEGORIES = "" CHIP_NAME = "530" FLOWS = 650 @@ -1857,7 +1818,7 @@ def add_or_update_hid_dexter_system_templates(): LIBRARY_READ_LENGTH = 200 REFERENCE = "hg19" SAMPLE_PREP_PROTOCOL = "" - SEQ_KIT_NAME = "HID S5 Sequencing Kit" + SEQ_KIT_NAME = "precisionIDS5Kit" TEMPLATE_KIT_NAME = "Ion Chef HID S530 V2" TEMPLATING_SIZE = None @@ -1903,6 +1864,456 @@ def add_or_update_hid_dexter_system_templates(): sysTemplate, isCreated, isUpdated = add_or_update_sys_template(templateParams) finish_sys_template(sysTemplate, isCreated, templateParams) + +def add_or_update_ocp_myeloid_pgm_system_templates(): + BARCODE_KIT_NAME = "IonXpress" + CATEGORIES_DNA = "barcodes_6;onco_heme" + CATEGORIES_RNA_FUSIONS = "barcodes_24;onco_heme" + CATEGORIES_DNA_n_FUSIONS = "barcodes_12;onco_heme" + CHIP_NAME = "318" + FLOWS = 850 + LIB_KIT_NAME = "Ion AmpliSeq Library Kit Plus" + LIBRARY_READ_LENGTH = 400 + REFERENCE = "hg19" + SAMPLE_GROUPING = "Self" + SAMPLE_PREP_PROTOCOL = "" + SEQ_KIT_NAME = "IonPGMHiQView" + TEMPLATE_KIT_NAME = "Ion PGM Hi-Q View Chef Kit" + ##TEMPLATING_SIZE = "400" + PLAN_STATUS = "inactive" + + # pre-select plugins + plugins = {} + plugins["coverageAnalysis"] = _get_plugin_dict("coverageAnalysis") + + # 67 + templateParams = TemplateParams("AmpliSeq Myeloid Research DNA for PGM", PGM, "AMPS") + templateParams.update({ + "applicationGroup" : "DNA", + "barcodeKitName": BARCODE_KIT_NAME, + "categories": CATEGORIES_DNA, + "chipType": CHIP_NAME, + "flows": FLOWS, + "libraryKitName": LIB_KIT_NAME, + "libraryReadLength" : LIBRARY_READ_LENGTH, + "reference": REFERENCE, + "sampleGrouping": SAMPLE_GROUPING, + "sequencekitname": SEQ_KIT_NAME, + "templatingKitName": TEMPLATE_KIT_NAME, + "planStatus": PLAN_STATUS + }) + sysTemplate, isCreated, isUpdated = add_or_update_sys_template(templateParams) + finish_sys_template(sysTemplate, isCreated, templateParams, plugins) + + # 68 + templateParams = TemplateParams("AmpliSeq Myeloid Research Fusions for PGM", PGM, "AMPS_RNA") + templateParams.update({ + "applicationGroup": "DNA + RNA", + "barcodeKitName": BARCODE_KIT_NAME, + "categories": CATEGORIES_RNA_FUSIONS, + "chipType": CHIP_NAME, + "flows": FLOWS, + "libraryKitName": LIB_KIT_NAME, + "libraryReadLength" : LIBRARY_READ_LENGTH, + "reference": REFERENCE, + "sampleGrouping": SAMPLE_GROUPING, + "sequencekitname": SEQ_KIT_NAME, + "templatingKitName": TEMPLATE_KIT_NAME, + "planStatus": PLAN_STATUS + }) + sysTemplate, isCreated, isUpdated = add_or_update_sys_template(templateParams) + finish_sys_template(sysTemplate, isCreated, templateParams, plugins) + + # 69 + templateParams = TemplateParams("AmpliSeq Myeloid Research DNA and Fusions for PGM", PGM, "AMPS_DNA_RNA") + templateParams.update({ + "applicationGroup": "DNA + RNA", + "barcodeKitName": BARCODE_KIT_NAME, + "categories": CATEGORIES_DNA_n_FUSIONS, + "chipType": CHIP_NAME, + "flows": FLOWS, + "libraryKitName": LIB_KIT_NAME, + "libraryReadLength" : LIBRARY_READ_LENGTH, + "reference": REFERENCE, + "sampleGrouping": "DNA and Fusions", + "sequencekitname": SEQ_KIT_NAME, + "templatingKitName": TEMPLATE_KIT_NAME, + "planStatus": PLAN_STATUS + }) + sysTemplate, isCreated, isUpdated = add_or_update_sys_template(templateParams) + finish_sys_template(sysTemplate, isCreated, templateParams, plugins) + + +def add_or_update_ocp_myeloid_s5_system_templates(): + BARCODE_KIT_NAME = "IonXpress" + CATEGORIES_DNA = "barcodes_12;onco_heme;chef_myeloid_protocol" + CATEGORIES_RNA_FUSIONS = "barcodes_48;onco_heme;chef_myeloid_protocol" + CATEGORIES_DNA_n_FUSIONS = "barcodes_24;onco_heme;chef_myeloid_protocol" + CHIP_NAME = "530" + FLOWS = 850 + LIB_KIT_NAME = "Ion AmpliSeq Library Kit Plus" + LIBRARY_READ_LENGTH = 400 + REFERENCE = "hg19" + SAMPLE_GROUPING = "Self" + SAMPLE_PREP_PROTOCOL = "denature30_cycles45_20" + SEQ_KIT_NAME = "Ion S5 Sequencing Kit" + TEMPLATE_KIT_NAME = "Ion Chef S530 V2" + ##TEMPLATING_SIZE = "400" + PLAN_STATUS = "inactive" + + # pre-select plugins + plugins = {} + plugins["coverageAnalysis"] = _get_plugin_dict("coverageAnalysis") + + # 70 + templateParams = TemplateParams("AmpliSeq Myeloid Research DNA for S5", S5, "AMPS") + templateParams.update({ + "applicationGroup" : "DNA", + "barcodeKitName": BARCODE_KIT_NAME, + "categories": CATEGORIES_DNA, + "chipType": CHIP_NAME, + "flows": FLOWS, + "libraryKitName": LIB_KIT_NAME, + "libraryReadLength" : LIBRARY_READ_LENGTH, + "reference": REFERENCE, + "sampleGrouping": SAMPLE_GROUPING, + "samplePrepProtocol": SAMPLE_PREP_PROTOCOL, + "sequencekitname": SEQ_KIT_NAME, + "templatingKitName": TEMPLATE_KIT_NAME, + "planStatus": PLAN_STATUS + }) + sysTemplate, isCreated, isUpdated = add_or_update_sys_template(templateParams) + finish_sys_template(sysTemplate, isCreated, templateParams, plugins) + + # 71 + templateParams = TemplateParams("AmpliSeq Myeloid Research Fusions for S5", S5, "AMPS_RNA") + templateParams.update({ + "applicationGroup": "DNA + RNA", + "barcodeKitName": BARCODE_KIT_NAME, + "categories": CATEGORIES_RNA_FUSIONS, + "chipType": CHIP_NAME, + "flows": FLOWS, + "libraryKitName": LIB_KIT_NAME, + "libraryReadLength" : LIBRARY_READ_LENGTH, + "reference": REFERENCE, + "sampleGrouping": SAMPLE_GROUPING, + "samplePrepProtocol": SAMPLE_PREP_PROTOCOL, + "sequencekitname": SEQ_KIT_NAME, + "templatingKitName": TEMPLATE_KIT_NAME, + "planStatus": PLAN_STATUS + }) + sysTemplate, isCreated, isUpdated = add_or_update_sys_template(templateParams) + finish_sys_template(sysTemplate, isCreated, templateParams, plugins) + + # 72 + templateParams = TemplateParams("AmpliSeq Myeloid Research DNA and Fusions for S5", S5, "AMPS_DNA_RNA") + templateParams.update({ + "applicationGroup": "DNA + RNA", + "barcodeKitName": BARCODE_KIT_NAME, + "categories": CATEGORIES_DNA_n_FUSIONS, + "chipType": CHIP_NAME, + "flows": FLOWS, + "libraryKitName": LIB_KIT_NAME, + "libraryReadLength" : LIBRARY_READ_LENGTH, + "reference": REFERENCE, + "sampleGrouping": "DNA and Fusions", + "samplePrepProtocol": SAMPLE_PREP_PROTOCOL, + "sequencekitname": SEQ_KIT_NAME, + "templatingKitName": TEMPLATE_KIT_NAME, + "planStatus": PLAN_STATUS + }) + sysTemplate, isCreated, isUpdated = add_or_update_sys_template(templateParams) + finish_sys_template(sysTemplate, isCreated, templateParams, plugins) + + +def add_or_update_proton_PQ_system_template(): + CATEGORIES = "" + CHIP = "P2.2.2" + FLOWS = 150 + LIBRARY_KIT_NAME = "Ion Xpress Plus Fragment Library Kit" + TEMPLATE_KIT_NAME = "Ion PQ Template OT2 Kit" + SEQ_KIT_NAME = "IonProtonPQKit" + BARCODE_KIT_NAME = "IonXpress" + PLAN_STATUS = "inactive" + + # 73 + templateParams = TemplateParams("Ion NIPT template - PQ", PROTON, "WGNM") + templateParams.update({ + "barcodeKitName": BARCODE_KIT_NAME, + "categories": CATEGORIES, + "chipType": CHIP, + "flows": FLOWS, + "libraryKitName": LIBRARY_KIT_NAME, + "reference": "hg19", + "sampleGrouping": "Self", + "sequencekitname": SEQ_KIT_NAME, + "templatingKitName": TEMPLATE_KIT_NAME, + "planStatus": PLAN_STATUS + }) + sysTemplate, isCreated, isUpdated = add_or_update_sys_template(templateParams) + finish_sys_template(sysTemplate, isCreated, templateParams) + + +def add_or_update_mouse_transcriptome_s5_system_templates(): + APPLICATION_GROUP = "RNA" + BARCODE_KIT_NAME = "IonCode Barcodes 1-32" + BARCODE_KIT_NAME_MANUAL = "IonXpress" + CATEGORIES = "" + CHIP = "540" + FLOWS = 500 + LIBRARY_KIT_NAME = "Ampliseq DNA V1" + LIBRARY_KIT_NAME_MANUAL = "Ion AmpliSeq Library Kit Plus" + LIBRARY_READ_LENGTH = 200 + REFERENCE = "mm10" + SAMPLE_GROUPING = "Self" + SEQ_KIT_NAME = "Ion S5 Sequencing Kit" + TEMPLATE_KIT_NAME = "Ion Chef S540 V1" + PLAN_STATUS = "inactive" + + # pre-select plugins + plugins = {} + plugins["ampliSeqRNA"] = _get_plugin_dict("ampliSeqRNA") + + # 74 + templateParams = TemplateParams("Ion AmpliSeq Transcriptome Mouse Gene Expression Chef-S5", S5, "AMPS_RNA") + templateParams.update({ + "applicationGroup": APPLICATION_GROUP, + "barcodeKitName": BARCODE_KIT_NAME, + "categories": CATEGORIES, + "chipType": CHIP, + "flows": FLOWS, + "libraryKitName": LIBRARY_KIT_NAME, + "libraryReadLength": LIBRARY_READ_LENGTH, + "reference": REFERENCE, + "sampleGrouping": SAMPLE_GROUPING, + "sequencekitname": SEQ_KIT_NAME, + "templatingKitName": TEMPLATE_KIT_NAME, + "planStatus": PLAN_STATUS + }) + sysTemplate, isCreated, isUpdated = add_or_update_sys_template(templateParams) + finish_sys_template(sysTemplate, isCreated, templateParams, plugins) + + # 75 + templateParams = TemplateParams("Ion AmpliSeq Transcriptome Mouse Gene Expression Manual Chef-S5", S5, "AMPS_RNA") + templateParams.update({ + "applicationGroup": APPLICATION_GROUP, + "barcodeKitName": BARCODE_KIT_NAME_MANUAL, + "categories": CATEGORIES, + "chipType": CHIP, + "flows": FLOWS, + "libraryKitName": LIBRARY_KIT_NAME_MANUAL, + "libraryReadLength": LIBRARY_READ_LENGTH, + "reference": REFERENCE, + "sampleGrouping": SAMPLE_GROUPING, + "sequencekitname": SEQ_KIT_NAME, + "templatingKitName": TEMPLATE_KIT_NAME, + "planStatus": PLAN_STATUS + }) + sysTemplate, isCreated, isUpdated = add_or_update_sys_template(templateParams) + finish_sys_template(sysTemplate, isCreated, templateParams, plugins) + + +def add_or_update_mouse_transcriptome_proton_system_templates(): + APPLICATION_GROUP = "RNA" + BARCODE_KIT_NAME = "IonXpress" + CATEGORIES = "" + CHIP = "P1.1.17" + FLOWS = 500 + LIBRARY_KIT_NAME = "Ion AmpliSeq Library Kit Plus" + REFERENCE = "mm10" + SAMPLE_GROUPING = "Self" + SEQ_KIT_NAME = " ProtonI200Kit-v3" + TEMPLATE_KIT_NAME = "Ion PI Template OT2 200 Kit v3" + PLAN_STATUS = "inactive" + + # pre-select plugins + plugins = {} + plugins["ampliSeqRNA"] = _get_plugin_dict("ampliSeqRNA") + + # 76 + templateParams = TemplateParams("Ion AmpliSeq Transcriptome Mouse Gene Expression Panel OT2-Proton", PROTON, "AMPS_RNA") + templateParams.update({ + "applicationGroup": APPLICATION_GROUP, + "barcodeKitName": BARCODE_KIT_NAME, + "categories": CATEGORIES, + "chipType": CHIP, + "flows": FLOWS, + "libraryKitName": LIBRARY_KIT_NAME, + "reference": REFERENCE, + "sampleGrouping": SAMPLE_GROUPING, + "sequencekitname": SEQ_KIT_NAME, + "templatingKitName": TEMPLATE_KIT_NAME, + "planStatus": PLAN_STATUS + }) + sysTemplate, isCreated, isUpdated = add_or_update_sys_template(templateParams) + finish_sys_template(sysTemplate, isCreated, templateParams, plugins) + + +def add_or_update_mutation_load_s5_system_templates(): + APPLICATION_GROUP = "DNA" + BARCODE_KIT_NAME = "IonCode Barcodes 1-32" + CATEGORIES = "onco_immune" + CHIP = "540" + FLOWS = 400 + LIBRARY_KIT_NAME = "Ion AmpliSeq Library Kit Plus" + LIBRARY_READ_LENGTH = 200 + REFERENCE = "hg19" + SAMPLE_GROUPING = "Self" + SEQ_KIT_NAME = "Ion S5 Sequencing Kit" + TEMPLATE_KIT_NAME = "Ion Chef S540 V1" + PLAN_STATUS = "inactive" + + # 77 + templateParams = TemplateParams("Oncomine Mutation Load", S5, "AMPS") + templateParams.update({ + "applicationGroup": APPLICATION_GROUP, + "barcodeKitName": BARCODE_KIT_NAME, + "categories": CATEGORIES, + "chipType": CHIP, + "flows": FLOWS, + "libraryKitName": LIBRARY_KIT_NAME, + "libraryReadLength": LIBRARY_READ_LENGTH, + "reference": REFERENCE, + "sampleGrouping": SAMPLE_GROUPING, + "sequencekitname": SEQ_KIT_NAME, + "templatingKitName": TEMPLATE_KIT_NAME, + "planStatus": PLAN_STATUS + }) + sysTemplate, isCreated, isUpdated = add_or_update_sys_template(templateParams) + finish_sys_template(sysTemplate, isCreated, templateParams) + + +def add_or_update_tagseq_cfdna_system_templates(): + LIQUID_BIOPSY_PLUGIN_CONFIG_FILENAME = "rundb/fixtures/systemtemplateparams/tagseq_liquidbiopsy_parameters.json" + TUMOR_PLUGIN_CONFIG_FILENAME = "rundb/fixtures/systemtemplateparams/tagseq_tumor_parameters.json" + + # 78 + sysTemplate, isCreated, isUpdated, templateParams = add_or_update_tagseq_cfdna_s5_chef_system_template("Oncomine TagSeq Tumor") + tumor_plugins = create_tagseq_plugins(TUMOR_PLUGIN_CONFIG_FILENAME) + templateParams.selectedPlugins = tumor_plugins + finish_sys_template(sysTemplate, isCreated, templateParams, tumor_plugins) + + # 79 + sysTemplate, isCreated, isUpdated, templateParams = add_or_update_tagseq_cfdna_s5_chef_system_template("Oncomine TagSeq Liquid Biopsy") + liquid_biopsy_plugins = create_tagseq_plugins(LIQUID_BIOPSY_PLUGIN_CONFIG_FILENAME) + templateParams.selectedPlugins = liquid_biopsy_plugins + finish_sys_template(sysTemplate, isCreated, templateParams, liquid_biopsy_plugins) + + + +def add_or_update_tagseq_cfdna_s5_chef_system_template(templateName): + # Tag Sequencing + TAG_SEQ_APPLICATION_GROUP = "onco_liquidBiopsy" + TAG_SEQ_BARCODE_KIT_NAME = "TagSequencing" + TAG_SEQ_CATEGORIES = "barcodes_8" + TAG_SEQ_CHIP_NAME = "530" + TAG_SEQ_FLOWS = 500 + TAG_SEQ_LIB_KIT_NAME = "Oncomine cfDNA Assay" + TAG_SEQ_LIBRARY_READ_LENGTH = 200 + TAG_SEQ_REFERENCE = "hg19" + TAG_SEQ_RUN_TYPE = "TAG_SEQUENCING" + TAG_SEQ_SAMPLE_GROUPING = "Self" + TAG_SEQ_SEQ_KIT_NAME = "Ion S5 Sequencing Kit" + TAG_SEQ_TEMPLATE_KIT_NAME = "Ion Chef S530 V1" + PLAN_STATUS = "planned" + + templateParams = TemplateParams(templateName, S5, TAG_SEQ_RUN_TYPE) + templateParams.update({ + "applicationGroup" : TAG_SEQ_APPLICATION_GROUP, + "barcodeKitName": TAG_SEQ_BARCODE_KIT_NAME, + "categories": TAG_SEQ_CATEGORIES, + "chipType": TAG_SEQ_CHIP_NAME, + "flows": TAG_SEQ_FLOWS, + "libraryKitName": TAG_SEQ_LIB_KIT_NAME, + "libraryReadLength" : TAG_SEQ_LIBRARY_READ_LENGTH, + "reference": TAG_SEQ_REFERENCE, + "sampleGrouping": TAG_SEQ_SAMPLE_GROUPING, + "sequencekitname": TAG_SEQ_SEQ_KIT_NAME, + "templatingKitName": TAG_SEQ_TEMPLATE_KIT_NAME, + "planStatus": PLAN_STATUS + }) + sysTemplate, isCreated, isUpdated = add_or_update_sys_template(templateParams) + return sysTemplate, isCreated, isUpdated, templateParams + + +def add_or_update_immune_repertoire_s5_system_templates(): + APPLICATION_GROUP = "immune_repertoire" + BARCODE_KIT_NAME = "IonXpress" + CATEGORIES = "onco_immune" + CHIP = "530" + FLOWS = 800 + LIBRARY_KIT_NAME = "Ion AmpliSeq Library Kit Plus" + LIBRARY_READ_LENGTH = 400 + REFERENCE = "" + SAMPLE_GROUPING = "Self" + SEQ_KIT_NAME = "Ion S5 Sequencing Kit" + TEMPLATE_KIT_NAME = "Ion Chef S530 V2" + PLAN_STATUS = "inactive" + + # pre-select plugins + plugins = {} + plugins["TCR_Repertoire"] = _get_plugin_dict("TCR_Repertoire") + + # 80 + templateParams = TemplateParams("Oncomine Immune Repertoire TCRB Assay - S5", S5, "AMPS_RNA") + templateParams.update({ + "applicationGroup": APPLICATION_GROUP, + "barcodeKitName": BARCODE_KIT_NAME, + "categories": CATEGORIES, + "chipType": CHIP, + "flows": FLOWS, + "libraryKitName": LIBRARY_KIT_NAME, + "libraryReadLength": LIBRARY_READ_LENGTH, + "reference": REFERENCE, + "sampleGrouping": SAMPLE_GROUPING, + "sequencekitname": SEQ_KIT_NAME, + "templatingKitName": TEMPLATE_KIT_NAME, + "planStatus": PLAN_STATUS + }) + + sysTemplate, isCreated, isUpdated = add_or_update_sys_template(templateParams) + finish_sys_template(sysTemplate, isCreated, templateParams, plugins) + + +def add_or_update_immune_repertoire_pgm_system_templates(): + APPLICATION_GROUP = "immune_repertoire" + BARCODE_KIT_NAME = "IonXpress" + CATEGORIES = "onco_immune" + CHIP = "318" + FLOWS = 800 + LIBRARY_KIT_NAME = "Ion AmpliSeq Library Kit Plus" + LIBRARY_READ_LENGTH = 400 + REFERENCE = "" + SAMPLE_GROUPING = "Self" + SEQ_KIT_NAME = "IonPGMHiQView" + TEMPLATE_KIT_NAME = "Ion PGM Hi-Q View Chef Kit" + PLAN_STATUS = "inactive" + + # pre-select plugins + plugins = {} + plugins["TCR_Repertoire"] = _get_plugin_dict("TCR_Repertoire") + + # 81 + templateParams = TemplateParams("Oncomine Immune Repertoire TCRB Assay - PGM", PGM, "AMPS_RNA") + templateParams.update({ + "applicationGroup": APPLICATION_GROUP, + "barcodeKitName": BARCODE_KIT_NAME, + "categories": CATEGORIES, + "chipType": CHIP, + "flows": FLOWS, + "libraryKitName": LIBRARY_KIT_NAME, + "libraryReadLength": LIBRARY_READ_LENGTH, + "reference": REFERENCE, + "sampleGrouping": SAMPLE_GROUPING, + "sequencekitname": SEQ_KIT_NAME, + "templatingKitName": TEMPLATE_KIT_NAME, + "planStatus": PLAN_STATUS + }) + + sysTemplate, isCreated, isUpdated = add_or_update_sys_template(templateParams) + finish_sys_template(sysTemplate, isCreated, templateParams, plugins) + + @transaction.commit_manually() def add_or_update_all_system_templates(): @@ -1929,6 +2340,15 @@ def add_or_update_all_system_templates(): add_or_update_S5_ocp_focus_system_templates() add_or_update_hid_system_templates() add_or_update_hid_dexter_system_templates() + add_or_update_ocp_myeloid_pgm_system_templates() + add_or_update_ocp_myeloid_s5_system_templates() + add_or_update_proton_PQ_system_template() + add_or_update_mouse_transcriptome_s5_system_templates() + add_or_update_mouse_transcriptome_proton_system_templates() + add_or_update_mutation_load_s5_system_templates() + add_or_update_tagseq_cfdna_system_templates() + add_or_update_immune_repertoire_s5_system_templates() + add_or_update_immune_repertoire_pgm_system_templates() except: print format_exc() transaction.rollback() @@ -1962,7 +2382,25 @@ def clean_up_obsolete_templates(): " Oncomine Comprehensive v2 for S5 DNA and Fusions", "Oncomine BRCA for PGM", "Oncomine BRCA for S5", - "Applied Biosystems Precision ID GlobalFiler NGS STR Panel" + "Applied Biosystems Precision ID GlobalFiler NGS STR Panel", + "Oncomine Comprehensive v3 DNA and Fusion ST", + "Oncomine Fusion ST", + "Ion ReproSeq Aneuploidy", + "Ion AmpliSeq Cancer Panel", + "AmpliSeq Myeloid DNA for PGM", + "AmpliSeq Myeloid Fusions for PGM", + "AmpliSeq Myeloid DNA and Fusions for PGM", + "AmpliSeq Myeloid DNA for S5", + "AmpliSeq Myeloid Fusions for S5", + "AmpliSeq Myeloid DNA and Fusions for S5", + "Ion ReproSeq - PGM", + "Ion ReproSeq - S5", + " Oncomine Lung Liquid Biopsy Total Nucleic Acid", + "Oncomine Mutational Load", + "Oncomine Lung Liquid Biopsy Total Nucleic Acid", + "Oncomine Lung Tumor Total Nucleic Acid", + "Oncomine Breast Liquid Biopsy DNA v2", + "Oncomine Breast Tumor DNA v2" ] templates = models.PlannedExperiment.objects.filter( @@ -2021,38 +2459,21 @@ def add_or_updateSystemTemplate_OffCycleRelease(**sysTemp): def pre_select_plugins(application, pluginsList): plugins = {} - pluginUserInput = {} - - thirdPartyPluginName = "PartekFlowUploader" - thirdPartyPluginDict = _get_third_party_plugin_dict(pluginId=9999, pluginName=thirdPartyPluginName) # pre-select plugins for plugin in pluginsList: - logger.debug("PreSelect plugin(%s) in the System Template " % plugin) - selectedPlugins = models.Plugin.objects.filter( - name__in=[plugin], selected=True, active=True).order_by("-id") - - for selectedPlugin in selectedPlugins: - pluginDict = { - "id": selectedPlugin.id, - "name": selectedPlugin.name, - "version": selectedPlugin.version, - "userInput": pluginUserInput, - "features": [] - } - plugins[selectedPlugin.name] = pluginDict - - if ((plugin == 'ampliSeqRNA') and (len(selectedPlugins) < 1)): - ionCommunityPluginDict = { - "id": 9999, - "name": "ampliSeqRNA", - "version": "1.0", - "userInput": pluginUserInput, - "features": [] - } - plugins["ampliSeqRNA"] = ionCommunityPluginDict + if isinstance(plugin, dict): + pluginName = plugin.get('name') + userInput = plugin.get('userInput') + else: + pluginName = plugin + userInput = {} + + plugins[pluginName] = _get_plugin_dict(pluginName, userInput) + if (application == "AMPS_RNA" or application == "RNA"): - plugins[thirdPartyPluginName] = thirdPartyPluginDict + thirdPartyPluginName = "PartekFlowUploader" + plugins[thirdPartyPluginName] = _get_plugin_dict(thirdPartyPluginName) return plugins diff --git a/dbReports/iondb/bin/check_system_services.py b/dbReports/iondb/bin/check_system_services.py new file mode 100644 index 00000000..9e92e7ec --- /dev/null +++ b/dbReports/iondb/bin/check_system_services.py @@ -0,0 +1,149 @@ +#!/usr/bin/python +# Copyright (C) 2017 Ion Torrent Systems, Inc. All Rights Reserved + +''' + This script can test essential TS system services are running and restart them + Usage: + no option: print status + -n/--notify: update webpage banner and send message to IT contact if any service is down + -s/--start: attempt to (re-)start all services, must be run as root +''' + +import os +import sys +sys.path.append('/opt/ion/') +os.environ['DJANGO_SETTINGS_MODULE'] = 'iondb.settings' + +import subprocess +import traceback +import argparse +from iondb.rundb.models import Message +from iondb.rundb.tasks import notify_services_error + +import logging +logger = logging.getLogger(__name__) + + +def process_status(): + # modified from /rundb/configure/views.py process_set() + def simple_status(name): + proc = subprocess.Popen("service %s status" % name, shell=True, stdout=subprocess.PIPE, stderr=subprocess.PIPE) + stdout, stderr = proc.communicate() + #logger.debug("%s out = '%s' err = %s''" % (name, stdout, stderr)) + return proc.returncode == 0 + + def complicated_status(filename, parse): + try: + if os.path.exists(filename): + data = open(filename).read() + pid = parse(data) + proc = subprocess.Popen("ps %d" % pid, shell=True, stdout=subprocess.PIPE, stderr=subprocess.PIPE) + proc.communicate() + return proc.returncode == 0 + except Exception as err: + return False + + proc_set = {} + processes = [ + "ionJobServer", + "ionCrawler", + "ionPlugin", + "apache2", + "postgresql" + ] + + for name in processes: + proc_set[name] = simple_status(name) + + proc_set["RabbitMQ"] = complicated_status("/var/run/rabbitmq/pid", int) + proc_set["gridengine"] = complicated_status("/var/run/gridengine/execd.pid", int) + + for node in ['celerybeat', 'celery_w1', 'celery_plugins', 'celery_periodic', 'celery_slowlane', 'celery_transfer', 'celery_diskutil']: + proc_set[node] = complicated_status("/var/run/celery/%s.pid" % node, int) + + alerts = [] + for process, active in sorted(proc_set.items(), key=lambda s: s[0].lower()): + print 'ok' if active else 'DOWN', process + if not active: + alerts.append(process) + + return alerts + + +def start_service(name): + cmd = "service %s restart" % name + print cmd + proc = subprocess.Popen(cmd, shell=True, stdout=subprocess.PIPE, stderr=subprocess.PIPE) + stdout, stderr = proc.communicate() + if proc.returncode: + print 'Error: %s returned %d, %s, %s' % (cmd, proc.returncode, stdout, stderr) + return proc.returncode == 0 + + +def update_banner(alerts): + new = False + # update message banner + message = Message.objects.filter(tags="service_status_alert") + if len(alerts) > 0: + msg = 'ALERT system services are down: %s. ' % ', '.join(alerts) + msg += ' Please contact your system administrator for assistance.' + if not message or message[0].body != msg: + message.delete() + Message.warn(msg, tags="service_status_alert") + new = True + else: + message.delete() + print '...updated message banner' + return new + + +def send_alert(alerts): + # send an email to IT contact + msg = "The following system services are down:\n" + for service in alerts: + msg += service + '\n' + notify_services_error('Torrent Server Alert', msg, msg.replace('\n','
')) + print '...notified IT list' + + + +if __name__ == '__main__': + parser = argparse.ArgumentParser(description="This script can check and restart essential TS services") + parser.add_argument('-n', '--notify', dest='notify', action='store_true', default=False, help='notify with banner and email alert') + parser.add_argument('-s', '--start', dest='start', action='store_true', default=False, help='attempt to (re-)start all services') + args = parser.parse_args() + + if args.start: + if os.geteuid() != 0: + sys.exit('Run this script with root permissions to start services') + + start_order = ['postgresql', + 'apache2', + 'gridengine-master', 'gridengine-exec', + 'rabbitmq-server', 'celeryd', 'celerybeat', + 'ionJobServer', 'ionCrawler', 'ionPlugin' + ] + for name in start_order: + start_service(name) + + # check status + alerts = process_status() + + if args.notify: + logger.info("check_system_services: %s" % alerts) + + try: + new = update_banner(alerts) + except: + logger.error('check_system_services: unable to update Message banner') + print traceback.format_exc() + else: + # send an email to IT contact + if alerts and new: + try: + send_alert(alerts) + except: + logger.error('check_system_services: unable to send email alert') + print traceback.format_exc() + + diff --git a/dbReports/iondb/bin/crawler.py b/dbReports/iondb/bin/crawler.py index 0bd9c00a..3e7269fb 100755 --- a/dbReports/iondb/bin/crawler.py +++ b/dbReports/iondb/bin/crawler.py @@ -37,9 +37,11 @@ import urllib import argparse -from iondb.bin.djangoinit import * +#from iondb.bin.djangoinit import * +from iondb.bin import djangoinit from django import db from django.db import connection +from django.conf import settings from twisted.internet import reactor from twisted.internet import task diff --git a/dbReports/iondb/bin/dj_config.py b/dbReports/iondb/bin/dj_config.py index c4fc2252..01f5963f 100644 --- a/dbReports/iondb/bin/dj_config.py +++ b/dbReports/iondb/bin/dj_config.py @@ -14,6 +14,7 @@ import os import subprocess +import requests def call(*cmd): @@ -32,3 +33,23 @@ def call(*cmd): def get_tmap_version(): return call("tmap", "index", "--version") + + +def is_s5_tsvm(): + """This method will return true if we are part of the S5 tsvm, false otherwise""" + # I am not super excited to use the existance of such a file as there could be a point + # in time in the future where the 1:1 correlation may change between the existance of + # this file and this TS being a S5 tsvm instance + return os.path.exists('/etc/init.d/mountExternal') + + +def get_s5_ip_addr(): + """get the S5 IP address""" + try: + resp = requests.get('http://192.168.122.1/instrument/software/www/config/DataCollect.config', timeout=2.0) + for line in resp.text.split('\n'): + if 'IP Address Str' in line: + return line[15:] + except requests.ConnectionError: + # we don't really need to do anything here asides from assume this is not an S5 and move on + return None diff --git a/dbReports/iondb/bin/from_wells_analysis.py b/dbReports/iondb/bin/from_wells_analysis.py index 7f269fdb..af8939e4 100755 --- a/dbReports/iondb/bin/from_wells_analysis.py +++ b/dbReports/iondb/bin/from_wells_analysis.py @@ -27,6 +27,7 @@ import logging from iondb.bin.djangoinit import * from iondb.rundb import models +from ion.utils.explogparser import load_log from ion.utils.explogparser import load_log_path from ion.utils.explogparser import parse_log from iondb.bin.crawler import generate_updateruninfo_post @@ -38,6 +39,12 @@ def __init__(self): self.errors = logging.getLogger(__name__) self.errors.propagate = True + def warn(self, msg): + print(msg) + + def error(self, msg): + print(msg) + logger = fakelog() TIMESTAMP_RE = models.Experiment.PRETTY_PRINT_RE @@ -72,7 +79,7 @@ def generate_http_post(exp, data_path, thumbnail_analysis=False): # instead of relying on globalConfig, user can now set isDuplicateReads for the experiment eas = exp.get_EAS() - if (eas): + if eas: # logger.errors.info("from_well_analysis.generate_http_post() exp.name=%s; # id=%s; isDuplicateReads=%s" %(exp.expName, str(exp.pk), # str(eas.isDuplicateReads))) @@ -136,9 +143,9 @@ def newExperiment(_explog_path, _plan_json=''): # Test if Experiment object already exists try: _newExp = models.Experiment.objects.get(unique=folder) - print "DEBUG: Experiment exists in database: %s" % (folder) + print("DEBUG: Experiment exists in database: %s" % folder) except: - print "DEBUG: Experiment does not exist in database" + print("DEBUG: Experiment does not exist in database") _newExp = None if _newExp is None: @@ -156,24 +163,50 @@ def newExperiment(_explog_path, _plan_json=''): # Experiment object exists in database _newExp = exp_set[0] else: - print "ERROR: Could not update/generate new Experiment record in database" - print ret_val + print("ERROR: Could not update/generate new Experiment record in database") + print(ret_val) return None # Append to expName to indicate imported dataset. _newExp.expName += "_foreign" + chefLog_parsed = isChefInfoAvailable(folder) + if chefLog_parsed: + update_chefSummary(_newExp, chefLog_parsed) _newExp.save() if _plan_json: planObj = _newExp.plan easObj = _newExp.get_EAS() update_plan_info(_plan_json, planObj, easObj) except: - print "DEBUG: There was an error adding the experiment" + print("DEBUG: There was an error adding the experiment") _newExp = None - print traceback.format_exc() + print(traceback.format_exc()) return _newExp +def isChefInfoAvailable(folder): + # parse chef_param.json + chefLog_parsed = {} + JSON_BASENAME = "chef_params.json" + chefLog = load_log(folder, JSON_BASENAME) + if chefLog is None: + payload = "Chef summary info not available read %s" % (os.path.join(folder, JSON_BASENAME)) + logger.warn(payload) + else: + try: + chefLog_parsed = json.loads(chefLog) + except: + logger.warn("Error parsing %s, skipping %s" % (JSON_BASENAME, folder)) + logger.error(traceback.format_exc()) + print(traceback.format_exc()) + + return chefLog_parsed + +def update_chefSummary(_newExp, chefSummary): + if chefSummary: + for k, v in chefSummary.items(): + if not v: continue + setattr(_newExp, k, v) def update_plan_info(_plan_json, planObj, easObj): # update Plan and EAS fields @@ -193,7 +226,7 @@ def update_plan_info(_plan_json, planObj, easObj): easObj.save() planObj.save() except: - print traceback.format_exc() + print(traceback.format_exc()) def getReportURL(_report_name): @@ -204,7 +237,7 @@ def getReportURL(_report_name): except models.Results.DoesNotExist: URLString = "Not found" except: - print traceback.format_exc() + print(traceback.format_exc()) finally: return URLString @@ -225,16 +258,16 @@ def getReportURL(_report_name): # Test inputs if not os.path.isdir(args.directory): - print "Does not exist: %s" % args.directory + print("Does not exist: %s" % args.directory) sys.exit(1) src_dir = args.directory # Validate existence of prerequisite files explog_path = os.path.join(src_dir, 'explog.txt') if not os.path.isfile(explog_path): - print "Does not exist: %s" % explog_path - print "Cannot create environment for re-analysis to take place" - print "STATUS: Error" + print("Does not exist: %s" % explog_path) + print("Cannot create environment for re-analysis to take place") + print("STATUS: Error") sys.exit(1) if os.path.exists(os.path.join(src_dir, 'onboard_results', 'sigproc_results')): @@ -252,39 +285,39 @@ def getReportURL(_report_name): else: wells_path = os.path.join(test_dir, '1.wells') if not os.path.isfile(wells_path): - print "Does not exist: %s" % wells_path - print "Cannot basecall without output from signal processing" - print "STATUS: Error" + print("Does not exist: %s" % wells_path) + print("Cannot basecall without output from signal processing") + print("STATUS: Error") sys.exit(1) testpath = os.path.join(test_dir, 'analysis.bfmask.bin') if not os.path.isfile(testpath): testpath = os.path.join(test_dir, 'bfmask.bin') if not os.path.isfile(testpath): - print "Does not exist: %s" % testpath - print "Cannot basecall without bfmask.bin from signal processing" - print "STATUS: Error" + print("Does not exist: %s" % testpath) + print("Cannot basecall without bfmask.bin from signal processing") + print("STATUS: Error") sys.exit(1) testpath = os.path.join(test_dir, 'analysis.bfmask.stats') if not os.path.isfile(testpath): testpath = os.path.join(test_dir, 'bfmask.stats') if not os.path.isfile(testpath): - print "Does not exist: %s" % testpath - print "Cannot basecall without bfmask.stats from signal processing" - print "STATUS: Error" + print("Does not exist: %s" % testpath) + print("Cannot basecall without bfmask.stats from signal processing") + print("STATUS: Error") sys.exit(1) # Missing these files just means key signal graph will not be generated testpath = os.path.join(test_dir, 'avgNukeTrace_ATCG.txt') if not os.path.isfile(testpath): - print "Does not exist: %s" % testpath - print "Cannot create TF key signal graph without %s file" % 'avgNukeTrace_ATCG.txt' + print("Does not exist: %s" % testpath) + print("Cannot create TF key signal graph without %s file" % 'avgNukeTrace_ATCG.txt') testpath = os.path.join(test_dir, 'avgNukeTrace_TCAG.txt') if not os.path.isfile(testpath): - print "Does not exist: %s" % testpath - print "Cannot create Library key signal graph without %s file" % 'avgNukeTrace_TACG.txt' + print("Does not exist: %s" % testpath) + print("Cannot create Library key signal graph without %s file" % 'avgNukeTrace_TACG.txt') # Plan parameters, if any plan_json = '' @@ -294,23 +327,23 @@ def getReportURL(_report_name): with open(plan_params_file) as f: plan_json = json.loads(f.read()) except: - print "Unable to read Plan info from ", plan_params_file + print("Unable to read Plan info from ", plan_params_file) # Create Experiment record newExp = newExperiment(explog_path, plan_json) if newExp is None: - print ("Could not create an experiment object") - print "STATUS: Error" + print("Could not create an experiment object") + print("STATUS: Error") sys.exit(1) # Submit analysis job URL report_name = generate_http_post(newExp, src_dir, thumbnail_analysis=args.thumbnail_only) if report_name == "Failure to generate POST": - print ("Could not start a new analysis") - print "STATUS: Error" + print("Could not start a new analysis") + print("STATUS: Error") sys.exit(1) else: - print ("DEBUG: Report Name is %s" % report_name) + print("DEBUG: Report Name is %s" % report_name) # Test for Report Object count = 0 @@ -320,17 +353,17 @@ def getReportURL(_report_name): count += 1 reportURL = getReportURL(report_name) if reportURL is None: - print "STATUS: Error" + print("STATUS: Error") sys.exit(1) elif reportURL == "Not found": - print "Retry %d of %d in %d second" % (count, retries, delay) + print("Retry %d of %d in %d second" % (count, retries, delay)) time.sleep(delay) else: count = retries if reportURL == "Not found": - print "STATUS: Error" + print("STATUS: Error") sys.exit(1) else: - print "STATUS: Success" - print "REPORT-URL: %s" % reportURL + print("STATUS: Success") + print("REPORT-URL: %s" % reportURL) diff --git a/dbReports/iondb/bin/grooming.py b/dbReports/iondb/bin/grooming.py new file mode 100755 index 00000000..1cf590c8 --- /dev/null +++ b/dbReports/iondb/bin/grooming.py @@ -0,0 +1,36 @@ +#!/usr/bin/env python + +"""This will go through old log files and groom them.""" + +import os +os.chdir('/opt/ion') +os.environ.setdefault("DJANGO_SETTINGS_MODULE", "iondb.settings") + +import simplejson as json +from iondb.rundb.models import PluginResult + +for result in PluginResult.objects.filter(plugin__name='RunTransfer'): + # groom the database entry + for plugin_result_job in result.plugin_result_jobs.all(): + if 'user_password' in plugin_result_job.config: + del plugin_result_job.config['user_password'] + plugin_result_job.save() + + # groom the log file + log_path = os.path.join(result.path(), "drmaa_stdout.txt") + if os.path.exists(log_path): + lines = [line for line in open(log_path) if 'user_password' not in line] + open(log_path, 'w').writelines(lines) + + # groom the startplugin.json on the file system + start_plugin_path = os.path.join(result.path(), "startplugin.json") + if os.path.exists(start_plugin_path): + start_plugin = dict() + with open(start_plugin_path) as start_plugin_fp: + start_plugin = json.load(start_plugin_fp) + + if 'pluginconfig' in start_plugin and 'user_password' in start_plugin['pluginconfig']: + del start_plugin['pluginconfig']['user_password'] + with open(start_plugin_path, 'w') as start_plugin_fp: + json.dump(start_plugin, start_plugin_fp, indent=4) + diff --git a/dbReports/iondb/bin/install_dmfilesets.py b/dbReports/iondb/bin/install_dmfilesets.py index 4133fa59..65cc3a69 100644 --- a/dbReports/iondb/bin/install_dmfilesets.py +++ b/dbReports/iondb/bin/install_dmfilesets.py @@ -18,10 +18,11 @@ # import sys import traceback -from djangoinit import * +from iondb.bin import djangoinit +from iondb import settings from iondb.rundb import models from iondb.rundb.data import dmactions_types - +#pylint: disable=W1401 DM_FILE_SETS = [ { 'type': dmactions_types.SIG, @@ -31,11 +32,14 @@ 'X\d+_Y\d+.*\.dat', 'thumbnail.*\.dat', 'thumbnail/explog_final.txt', + 'thumbnail/explog_final.json', #'.[^/]*?\.txt', need to be specific '.[^/]*?alW1Step_explog\.txt', '.[^/]*?hecksum_status\.txt', '.[^/]*?xplog\.txt', + '.[^/]*?xplog\.json', '.[^/]*?xplog_final\.txt', + '.[^/]*?xplog_final\.json', '.[^/]*?nitLog.*?txt', '.[^/]*?nitValsW.*?txt', '.[^/]*?awInit\.txt', @@ -59,6 +63,7 @@ 'keepwith':{ dmactions_types.BASE: [ '.[^/]*?xplog\.txt', + '.[^/]*?xplog\.json', ], }, 'version': settings.RELVERSION, @@ -97,16 +102,19 @@ 'onboard_results/sigproc_results/avgNukeTrace_TCAG\.txt', 'onboard_results/sigproc_results/Bead_density_.*?\.png', '.[^/]*?xplog\.txt', + '.[^/]*?xplog\.json', ], 'exclude':[ ], 'keepwith':{ dmactions_types.OUT: [ 'sigproc_results/analysis\.bfmask\.stats', + 'onboard_results/sigproc_results/analysis\.bfmask\.stats', 'sigproc_results/Bead_density_.*?\.png', ], dmactions_types.SIG:[ '.[^/]*?xplog\.txt', + '.[^/]*?xplog\.json', ], }, 'version': settings.RELVERSION, @@ -151,6 +159,7 @@ 'basecaller_results/.[^/]*\.bam', 'sigproc_results/.[^/]*?\.json', 'sigproc_results/analysis.bfmask.stats', + 'onboard_results/sigproc_results/analysis\.bfmask\.stats', 'sigproc_results/Bead_density_.*?\.png', 'bc_files/.*?', 'bc_filtered/.*?', @@ -170,6 +179,7 @@ 'keepwith':{ dmactions_types.BASE: [ 'sigproc_results/analysis.bfmask.stats', + 'onboard_results/sigproc_results/analysis\.bfmask\.stats', 'sigproc_results/Bead_density_.*?\.png', ], }, @@ -211,10 +221,13 @@ 'X\d+_Y\d+.*\.dat', 'thumbnail.*\.dat', 'thumbnail/explog_final.txt', + 'thumbnail/explog_final.json', '.*?CalW1Step_explog\.txt', '.*?checksum_status\.txt', '.*?explog\.txt', + '.*?explog\.json', '.*?explog_final\.txt', + '.*?explog_final\.json', '.*?InitLog.*?txt', '.*?InitValsW.*?txt', '.*?RawInit\.txt', diff --git a/dbReports/iondb/bin/install_new_tsvm.py b/dbReports/iondb/bin/install_new_tsvm.py new file mode 100755 index 00000000..381d8640 --- /dev/null +++ b/dbReports/iondb/bin/install_new_tsvm.py @@ -0,0 +1,44 @@ +#!/usr/bin/env python +# Copyright (C) 2016 Ion Torrent Systems, Inc. All Rights Reserved + +""" +This will install or upgrade the tsvm package +""" + +import json +import os +import sys + +TSVM_PACKAGE = 'ion-tsvm' + +# turn off complex traceback stuff +sys.tracebacklimit = 0 + +# check for root level permissions +if os.geteuid() != 0: + sys.exit("You need to have root privileges to run this script.\nPlease try again, this time using 'sudo'. Exiting.") + +# NOTE: Must be run with root privileges +response_obj = {'action': 'update'} +try: + import pkg_resources + from distutils.version import LooseVersion + import apt + apt_cache = apt.Cache() + apt_cache.update() + apt_cache.open() + + if apt_cache.has_key(TSVM_PACKAGE): + pkg = apt_cache[TSVM_PACKAGE] + + if bool(pkg.is_installed): + pkg.mark_upgrade() + else: + pkg.mark_install() + + apt_cache.commit() + response_obj.update({'state': 'Success', 'msg': "ion-tsvm updated"}) +except Exception as e: + response_obj.update({'state': 'Error', 'msg': str(e)}) + +print(json.dumps(response_obj)) diff --git a/dbReports/iondb/bin/install_script.py b/dbReports/iondb/bin/install_script.py index 0352cde1..78c92710 100755 --- a/dbReports/iondb/bin/install_script.py +++ b/dbReports/iondb/bin/install_script.py @@ -41,6 +41,15 @@ def create_user_profiles(): print "Added missing userprofile for: %s" % user.username +def default_location(): + loc = models.Location.objects.filter(defaultlocation=True) or models.Location.objects.filter(name='Home') + if loc: + loc = loc[0] + else: + loc = models.Location.objects.all()[0] + return loc + + def add_fileserver(_name, _path): fs = models.FileServer.objects.all() if len(fs) == 0: @@ -58,7 +67,7 @@ def add_fileserver(_name, _path): if not exists: fs = models.FileServer(name=_name, filesPrefix=_path, - location=models.Location.objects.all()[0]) + location=default_location() ) fs.save() else: @@ -180,15 +189,11 @@ def add_global_config(configs): defaultStore = 'A' kwargs = {'name': 'Config', 'selected': False, - 'plugin_folder': 'plugins', - 'fasta_path': '', - 'reference_path': '', 'records_to_display': 20, 'default_test_fragment_key': 'ATCG', 'default_library_key': 'TCAG', 'default_flow_order': 'TACG', 'plugin_output_folder': 'plugin_out', - 'default_plugin_script': 'launch.sh', 'web_root': '', 'site_name': 'Torrent Server', 'default_storage_options': defaultStore, @@ -215,38 +220,6 @@ def runtype_add_obsolete(type, description): # print type, " RunType added" -def VariantFrequencies_add(name): - """Helper function to add VariantFrequencies""" - - vf = models.VariantFrequencies.objects.filter(name=name) - - if vf: - # print "VariantFrequency" , vf[0], "exists" - pass - else: - vf = models.VariantFrequencies(name=name) - vf.save() - # print name, " VariantFrequency added" - - -def create_default_pgm(pgmname, comment=''): - pgms = models.Rig.objects.all() - for pgm in pgms: - if pgm.name == pgmname: - # print "PGM named %s already exists" % pgmname - return True - locs = models.Location.objects.all() - if locs: - loc = locs[locs.count() - 1] - pgm = models.Rig(name=pgmname, - location=loc, - comments=comment) - pgm.save() - else: - print "Error: No Location object exists in database!" - return 1 - - def add_or_update_barcode_set(blist, btype, name, adapter): # Attempt to read dnabarcode set named 'IonXpress' from dbase dnabcs = models.dnaBarcode.objects.filter(name=name) @@ -1542,13 +1515,13 @@ def add_or_update_ioncode_tagseq_dnabarcode_set(): # mark barcodes 1-96 within a plate. index = 0 index = add_or_update_barcode_set2( - blist[0: 96], btype, name, adapter, 1, 2.00, barcode_prefix, False, 4, 101, index, True) + blist[0: 96], btype, name, adapter, 1, 2.00, barcode_prefix, False, 4, 101, index, False) index = add_or_update_barcode_set2( - blist[96: 96 * 2], btype, name, adapter, 1, 2.00, barcode_prefix, False, 4, 201, index, True) + blist[96: 96 * 2], btype, name, adapter, 1, 2.00, barcode_prefix, False, 4, 201, index, False) index = add_or_update_barcode_set2( - blist[96 * 2: 96 * 3], btype, name, adapter, 1, 2.00, barcode_prefix, False, 4, 301, index, True) + blist[96 * 2: 96 * 3], btype, name, adapter, 1, 2.00, barcode_prefix, False, 4, 301, index, False) index = add_or_update_barcode_set2( - blist[96 * 3: 96 * 4], btype, name, adapter, 1, 2.00, barcode_prefix, False, 4, 401, index, True) + blist[96 * 3: 96 * 4], btype, name, adapter, 1, 2.00, barcode_prefix, False, 4, 401, index, False) return @@ -1778,25 +1751,6 @@ def add_libraryKey(direction, name, sequence, description, isDefault): libKey.save() -def add_barcode_args(): - # print "Adding barcode_args" - try: - gc = models.GlobalConfig.get() - try: - barcode_args = gc.barcode_args - except AttributeError: - print "barcode_args field does not exist" - return 1 - # Only add the argument if it does not exist - if "doesnotexist" in str(gc.barcode_args.get('filter', "doesnotexist")): - gc.barcode_args['filter'] = 0.01 - gc.save() - print "added barcodesplit:filter" - except: - print "There is no GlobalConfig object in database" - print traceback.format_exc() - - def add_sequencing_kit_info(name, description, flowCount): # print "Adding sequencing kit info" try: @@ -1986,21 +1940,11 @@ def check_bool(value): # print 'Adding runType failed' # print traceback.format_exc() # sys.exit(1) - # try to add variant frequencies - try: - VariantFrequencies_add("Germ Line") - VariantFrequencies_add("Somatic") - except: - print 'Adding VariantFrequencies failed' - print traceback.format_exc() - sys.exit(1) # try to add PGMs try: - create_default_pgm('default', comment="This is a model PGM. Do not delete.") - # causes errors if system is not completely configured and auto-analysis - # kicks off. - # create_default_pgm('PGM_test',comment = "This is a test pgm for staging sample datasets.") + models.Rig.objects.get_or_create(name = 'default', defaults={'location': default_location(), + 'comments': "This is a model PGM. Do not delete." }) except: print 'Adding default PGM failed' print traceback.format_exc() @@ -2104,13 +2048,6 @@ def check_bool(value): # print traceback.format_exc() # sys.exit(1) - try: - add_barcode_args() - except: - print "Modifying barcode-args list failed" - print traceback.format_exc() - sys.exit(1) - try: add_libraryKey('Forward', 'Ion TCAG', 'TCAG', 'Default forward library key', True) add_libraryKey('Reverse', 'Ion Paired End', 'TCAGC', 'Default reverse library key', True) @@ -2130,5 +2067,22 @@ def check_bool(value): for chip in models.Chip.objects.all(): chip.delete() + load_dbData("rundb/fixtures/ts_dbData_chips_kits.json") load_dbData("rundb/fixtures/ts_dbData.json") + load_dbData("rundb/fixtures/ts_dbData_analysisargs.json") load_dbData("rundb/fixtures/ionusers_group.json") + + # Setup an ion mesh user for mesh authed api calls to use + load_dbData("rundb/fixtures/ionmesh_group.json") + try: + user, is_newly_added = add_user("ionmesh", "ionmesh") + if is_newly_added: + user.set_unusable_password() + group = Group.objects.get(name='ionmesh') + if group and user.groups.count() == 0: + user.groups.add(group) + user.save() + except: + print 'Adding ionmesh user failed' + print traceback.format_exc() + sys.exit(1) diff --git a/dbReports/iondb/bin/ionPlugin.py b/dbReports/iondb/bin/ionPlugin.py index 5c10919c..b6f86735 100755 --- a/dbReports/iondb/bin/ionPlugin.py +++ b/dbReports/iondb/bin/ionPlugin.py @@ -26,7 +26,7 @@ from iondb.plugins.launch_utils import get_plugins_to_run, add_hold_jid from iondb.plugins.plugin_json import make_plugin_json -from iondb.rundb.models import Results, PluginResult, User, Plugin +from iondb.rundb.models import Results, PluginResult, PluginResultJob, User, Plugin from django.db import IntegrityError from ion.plugin.constants import Feature, RunLevel @@ -172,16 +172,15 @@ def SGEPluginJob(start_json, hold=False): args is a dict of all the args that are needed by for the plugin to run """ try: - os.umask(0000) + os.umask(0o0002) plugin_output = start_json['runinfo']['results_dir'] # Make sure the dirs exist if not os.path.exists(plugin_output): - os.makedirs(plugin_output, 0775) + os.makedirs(plugin_output, 0o0775) plugin = start_json['runinfo']['plugin'] - logger.info("Preparing for SGE submission - plugin %s --v%s on result %s (%s)", plugin[ - 'name'], plugin['version'], start_json['expmeta']['results_name'], start_json['runinfo']['pk']) + logger.info("Preparing for SGE submission - plugin %s --v%s on result %s (%s)", plugin['name'], plugin['version'], start_json['expmeta']['results_name'], start_json['runinfo']['pk']) # Branch for launch.sh vs 3.0 plugin class # logger.debug("Finding plugin definition: '%s':'%s'", plugin['name'], plugin['path']) @@ -318,35 +317,20 @@ def GetSupportedPluginInfo(self, pluginPackageName): return plugin - def pluginStart(self, start_json): - """ - Launch the plugin defined by the start_json block - """ - logger.debug("SGE job start request") - try: - jobid = SGEPluginJob(start_json, hold=True) - _session.control(jid, drmaa.JobControlAction.RELEASE) # no return value - return jobid - except: - logger.error(traceback.format_exc()) - return -1 - def launchPlugins(self, result_pk, plugins, net_location, username, runlevel=RunLevel.DEFAULT, params={}): """ Launch multiple plugins with dependencies For multi-runlevel plugins the input 'plugins' is common for all runlevels """ msg = '' - logger.debug("[launchPlugins] result %s requested plugins: %s" % - (result_pk, ','.join(plugins.keys()))) + logger.debug("[launchPlugins] result %s requested plugins: %s" % (result_pk, ','.join(plugins.keys()))) try: # get plugins to run for this runlevel plugins, plugins_to_run, satisfied_dependencies = get_plugins_to_run(plugins, result_pk, runlevel) if len(plugins_to_run) > 0: - logger.debug("[launchPlugins] runlevel: %s, depsolved launch order: %s" % - (runlevel, ','.join(plugins_to_run))) + logger.debug("[launchPlugins] runlevel: %s, depsolved launch order: %s" % (runlevel, ','.join(plugins_to_run))) else: logger.debug("[launchPlugins] no plugins to run at runlevel: %s" % runlevel) return plugins, msg @@ -360,8 +344,7 @@ def launchPlugins(self, result_pk, plugins, net_location, username, runlevel=Run user = User.objects.get(username=username) except User.DoesNotExist: user = User.objects.get(pk=1) - logger.error("Invalid user specified for plugin launch: %s, will use %s" % - (username, user.username)) + logger.error("Invalid user specified for plugin launch: %s, will use %s" % (username, user.username)) for name in plugins_to_run: try: @@ -369,8 +352,7 @@ def launchPlugins(self, result_pk, plugins, net_location, username, runlevel=Run # get params for this plugin, make empty json value if doesn't exist plugin_params = params.setdefault('plugins', {}).setdefault(name, {}) - # Get pluginresult for multi-runlevel plugins or if specified to be reused - # by manual launch + # Get pluginresult for multi-runlevel plugins or if specified to be reused by manual launch pr = None pluginresult_pk = p.get('pluginresult') or plugin_params.get('pluginresult') @@ -379,21 +361,13 @@ def launchPlugins(self, result_pk, plugins, net_location, username, runlevel=Run try: pr = result.pluginresult_set.get(pk=pluginresult_pk) except: - logger.error("Failed to find pluginresult for plugin %s, result %s: %s" % - (name, result.resultsName, pluginresult_pk)) + logger.error("Failed to find pluginresult for plugin %s, result %s: %s" % (name, result.resultsName, pluginresult_pk)) pr = None - elif Feature.EXPORT in p.get('features', []): - # Export plugins rerun in place to enable resuming upload - pr = result.pluginresult_set.filter(plugin=p['id']) - if pr.count() > 0: - pr = pr[0] - pr.owner = user # Create new pluginresult - this is the most common path if not pr: pr = PluginResult.objects.create(result_id=result_pk, plugin_id=p['id'], owner=user) - logger.debug("New pluginresult id=%s created for plugin %s and result %s." % - (pr.pk, name, result.resultsName)) + logger.debug("New pluginresult id=%s created for plugin %s and result %s." % (pr.pk, name, result.resultsName)) # Always create new, unique output folder. # Never fallback to old *_out format. plugin_output = pr.path(create=True, fallback=False) @@ -405,8 +379,8 @@ def launchPlugins(self, result_pk, plugins, net_location, username, runlevel=Run p['pluginresult'] = pr.pk p, holding_for = add_hold_jid(p, plugins, runlevel, satisfied_dependencies) - start_json = make_plugin_json(result_pk, report_dir, p, plugin_output, net_location, url_root, username, runlevel, params.get( - 'blockId', ''), params.get('block_dirs', ["."]), plugin_params.get('instance_config', {})) + start_json = make_plugin_json(result_pk, report_dir, p, plugin_output, net_location, url_root, username, runlevel, + params.get('blockId', ''), params.get('block_dirs', ["."]), plugin_params.get('instance_config', {})) # Pass on run_mode (launch source - manual/instance, pipeline) start_json['runplugin']['run_mode'] = params.get('run_mode', '') @@ -425,7 +399,7 @@ def launchPlugins(self, result_pk, plugins, net_location, username, runlevel=Run } # prepare for launch: updates config, sets pluginresults status, generates api key - pr.prepare(config=start_json['pluginconfig']) + pr.prepare() pr.save() # update startplugin json with pluginresult info start_json['runinfo']['pluginresult'] = pr.pk @@ -438,12 +412,19 @@ def launchPlugins(self, result_pk, plugins, net_location, username, runlevel=Run if jid: # Update pluginresult status - PluginResult.objects.filter(pk=pr.pk).update(state='Queued', jobid=jid) + prj, created = PluginResultJob.objects.get_or_create( + plugin_result=pr, + run_level=runlevel, + grid_engine_jobid=jid, + state = 'Queued', + config = start_json['pluginconfig'], + ) + prj.save() + # Release now that jobid and queued state are set. _session.control(jid, drmaa.JobControlAction.RELEASE) # no return value - msg += 'Plugin: %s result: %s, jid %s, depends %s, holding for %s \n' % ( - p.get('name', ''), result.resultsName, jid, p.get('depends', []), holding_for) + msg += 'Plugin: %s result: %s, jid %s, depends %s, holding for %s \n' % (p.get('name', ''), result.resultsName, jid, p.get('depends', []), holding_for) if runlevel != RunLevel.BLOCK: p['jid'] = jid @@ -454,8 +435,6 @@ def launchPlugins(self, result_pk, plugins, net_location, username, runlevel=Run logger.error(traceback.format_exc()) msg += 'ERROR: Plugin %s failed to launch.\n' % p['name'] pr = PluginResult.objects.get(pk=pr.pk) - pr.complete('Error') - pr.save() except: logger.error(traceback.format_exc()) msg += 'ERROR: Failed to launch requested plugins.' @@ -477,10 +456,8 @@ def sgeStop(self, jobid): try: jobinfo = _session.wait(jobid, timeout=20) # returns JobInfo object - logger.info("Job %s wasAborted=%s with exit_status=%s", jobinfo.jobId, - jobinfo.wasAborted, jobinfo.resourceUsage.get('exit_status')) - status = "Job %s wasAborted=%s with exit_status=%s" % ( - jobinfo.jobId, jobinfo.wasAborted, jobinfo.resourceUsage.get('exit_status')) + logger.info("Job %s wasAborted=%s with exit_status=%s", jobinfo.jobId, jobinfo.wasAborted, jobinfo.resourceUsage.get('exit_status')) + status = "Job %s wasAborted=%s with exit_status=%s" % (jobinfo.jobId, jobinfo.wasAborted, jobinfo.resourceUsage.get('exit_status')) except drmaa.errors.InvalidJobException: status = "Job already terminated, or started previously." logger.warn("SGE job %s already terminated", jobid) @@ -519,24 +496,7 @@ def pluginStatus(self, jids): return ret if return_list else ret[0] - def createPR(self, resultpk, pluginpk, username=None, config={}): - if username is None: - username = "ionadmin" - try: - user = User.objects.get(username=username) - except User.DoesNotExist: - user = User.objects.get(pk=1) - try: - pr = PluginResult.objects.create( - result_id=resultpk, plugin_id=pluginpk, owner=user, pluginconfig=config) - except IntegrityError: - return False - # from tastypie.serializers import Serializer - # s = Serializer() - # return s.to_json(pr) - return True - - def updatePR(self, pk, state, store, jobid=None): + def updatePR(self, pk, grid_engine_job_id, state, store, jobid=None): """ Because of the Apache security it is hard for crucher nodes to contact the API directly. This method is a simple proxy to update the status of plugins @@ -548,8 +508,8 @@ def updatePR(self, pk, state, store, jobid=None): # TODO: should do an explicit check for the database object in case it's been deleted pr = PluginResult.objects.get(id=pk) - if (state is not None) and (state != pr.state): - pr.SetState(state, jobid) + if state is not None: + pr.SetState(state, grid_engine_job_id, jobid) if store is not None: # Validate JSON? @@ -561,6 +521,13 @@ def updatePR(self, pk, state, store, jobid=None): return True + def delete_pr_directory(self, directory): + """We need to use the same user to delete the folder as created it""" + def delete_error(func, path, info): + logger.error("Failed to delete %s: %s", path, info) + + shutil.rmtree(directory, onerror=delete_error) + if __name__ == '__main__': # Instantiate the xmlrpc server server = AsyncXMLRPCServer(('', settings.IPLUGIN_PORT), SimpleXMLRPCRequestHandler, allow_none=True) diff --git a/dbReports/iondb/bin/ion_add_nfs_mount.py b/dbReports/iondb/bin/ion_add_nfs_mount.py new file mode 100644 index 00000000..25616976 --- /dev/null +++ b/dbReports/iondb/bin/ion_add_nfs_mount.py @@ -0,0 +1,65 @@ +#!/usr/bin/env python +# Copyright (C) 2016 Ion Torrent Systems, Inc. All Rights Reserved + +""" +This module will install a new nfs mount + +This module will require three arguments +1) Server name: The host or ip address of the nas server +2) Share name: The name of the share to be mounted +3) Mount point: The place on the local file system to mount it. +""" + +import os +import shutil +import sys +import subprocess + + +# turn off complex traceback stuff +sys.tracebacklimit = 0 + +# check for root level permissions +if os.geteuid() != 0: + sys.exit("You need to have root privileges to run this script.\nPlease try again, this time using 'sudo'. Exiting.") + +if len(sys.argv) < 4: + sys.exit("Not enough arguments..") + +# get the arguments +servername = sys.argv[1] +sharename = sys.argv[2] +mountpoint = sys.argv[3] + +# create all_local from all +filename = "/usr/share/ion-tsconfig/ansible/group_vars/all_local" +tempfile_path = "/usr/share/ion-tsconfig/ansible/group_vars/all_local.temp" +if not os.path.isfile(filename): + shutil.copy2("/usr/share/ion-tsconfig/ansible/group_vars/all", filename) +# Line to add - spaces are important! +new_nas = " - { name: %s, directory: %s, mountpoint: %s, options: %s }" % (servername, sharename, mountpoint, "defaults") +# Check if line exists. This is a small file so we can do this cheat +with open(filename, 'r') as fh: + if new_nas in fh.read(): + print("This line: '%s'. Already exists in all_local." % new_nas) + else: + fh.seek(0) + # Insert line after pattern ^nas_mounts: + with open(tempfile_path, "w") as out: + for line in fh: + out.write(line) + if 'nas_mounts:' in line: + out.write(new_nas + '\n') + shutil.move(tempfile_path, filename) + +# Which hosts file to use +hosts_file = '/usr/share/ion-tsconfig/ansible/torrentsuite_hosts' +if os.path.isfile(hosts_file + '_local'): + hosts_file += '_local' + +# Run ansible-playbook in a subshell since running it from python celery task is broken +cmd = ["ansible-playbook", "-i", hosts_file, 'nfs_client.yml', "--sudo"] +p = subprocess.Popen(cmd, cwd="/usr/share/ion-tsconfig/ansible", stdout=subprocess.PIPE, stderr=subprocess.PIPE) +stdout, stderr = p.communicate() +sys.stdout.write(stdout) +sys.stderr.write(stderr) \ No newline at end of file diff --git a/dbReports/iondb/bin/ion_check_for_new_tsvm.py b/dbReports/iondb/bin/ion_check_for_new_tsvm.py new file mode 100644 index 00000000..0eb0d594 --- /dev/null +++ b/dbReports/iondb/bin/ion_check_for_new_tsvm.py @@ -0,0 +1,34 @@ +#!/usr/bin/env python +# Copyright (C) 2016 Ion Torrent Systems, Inc. All Rights Reserved + +""" +This module will check to see if there is a new version of tsvm available +""" + +import apt +import json +import os +import sys + +# turn off complex traceback stuff +sys.tracebacklimit = 0 + +# check for root level permissions +if os.geteuid() != 0: + sys.exit("You need to have root privileges to run this script.\nPlease try again, this time using 'sudo'. Exiting.") + +# update the aptitude cache +apt_cache = apt.Cache() +apt_cache.update() +apt_cache.open() + +# get the available version and current state +state = 'Not Available' +msg = 'No ion-tsvm packages are available for installation.' +if apt_cache.has_key('ion-tsvm'): + pkg = apt_cache['ion-tsvm'] + msg = 'New ion-tsvm package version %s is available' % pkg.candidate if pkg.is_upgradable else 'ion-tsvm is up to date.' + state = 'Upgradable' if pkg.is_upgradable else 'Not Upgradable' + +# write to stdout +print(json.dumps({'state': state, 'msg': msg})) \ No newline at end of file diff --git a/dbReports/iondb/bin/ion_remove_nfs_mount.py b/dbReports/iondb/bin/ion_remove_nfs_mount.py new file mode 100644 index 00000000..bb4f7b99 --- /dev/null +++ b/dbReports/iondb/bin/ion_remove_nfs_mount.py @@ -0,0 +1,48 @@ +#!/usr/bin/env python +# Copyright (C) 2016 Ion Torrent Systems, Inc. All Rights Reserved + +""" +This module will remove an nfs mount + +This will require one argument which is the mount point +""" + +import os +import shutil +import sys +import subprocess + + +# turn off complex traceback stuff +sys.tracebacklimit = 0 + +# check for root level permissions +if os.geteuid() != 0: + sys.exit("You need to have root privileges to run this script.\nPlease try again, this time using 'sudo'. Exiting.") + +if len(sys.argv) < 2: + sys.exit("Not enough arguments..") + +# get the arguments +mountpoint = sys.argv[1] + +filename = "/usr/share/ion-tsconfig/ansible/group_vars/all_local" +tempfile = "/tmp/all_local.temp" +# Delete the line matching the pattern +with open(tempfile, "w") as out, open(filename, "r") as fh: + for line in fh: + if mountpoint not in line: + out.write(line) +shutil.move(tempfile, filename) + +# Which hosts file to use +hosts_file = '/usr/share/ion-tsconfig/ansible/torrentsuite_hosts' +if os.path.isfile(hosts_file + '_local'): + hosts_file += '_local' + +# Run ansible-playbook in a subshell since running it from python celery task is broken +cmd = ["ansible-playbook", "-i", hosts_file, 'nfs_client.yml', "--sudo"] +p = subprocess.Popen(cmd, cwd="/usr/share/ion-tsconfig/ansible", stdout=subprocess.PIPE, stderr=subprocess.PIPE) +stdout, stderr = p.communicate() +sys.stdout.write(stdout) +sys.stderr.write(stderr) \ No newline at end of file diff --git a/dbReports/iondb/bin/lock_ion_apt_sources.py b/dbReports/iondb/bin/lock_ion_apt_sources.py new file mode 100644 index 00000000..a53aae55 --- /dev/null +++ b/dbReports/iondb/bin/lock_ion_apt_sources.py @@ -0,0 +1,59 @@ +#!/usr/bin/env python +# Copyright (C) 2016 Ion Torrent Systems, Inc. All Rights Reserved + +""" +This script will lock or unlock the version of the ion software by manipulating the apt source path. +""" +from ion import version +import os +import subprocess +import sys + +# turn off complex traceback stuff +sys.tracebacklimit = 0 + +# check for root level permissions +if os.geteuid() != 0: + sys.exit("You need to have root privileges to run this script.\nPlease try again, this time using 'sudo'. Exiting.") + +# look for the file name +if len(sys.argv) < 2: + sys.exit("Enable flag is required") + +# parse the first argument to a bool +enable = sys.argv[1].lower() == 'true' + +# get distribution string +os_codename = 'trusty' +with open('/etc/lsb-release', 'r') as fp: + for line in fp.readlines(): + if line.startswith('DISTRIB_CODENAME'): + os_codename = line.split('=')[1].strip() + +if enable: + find_string = "updates\/software.*%s\/" % os_codename + replace_string = "updates\/software\/archive\/%s %s\/" % (version, os_codename) +else: + find_string = "updates\/software\/archive\/%s.*" % version + replace_string = "updates\/software %s\/" % os_codename +sed_string = "s/%s/%s/g" % (find_string, replace_string) + +# Possible locations of Ion Apt repository strings: +# /etc/apt/sources.list +# /etc/apt/sources.list.d/*.list +# +filepaths = [os.path.join("/etc/apt/sources.list.d", x) for x in os.listdir("/etc/apt/sources.list.d") if os.path.splitext(x)[1] == '.list'] +filepaths.append("/etc/apt/sources.list") +for filepath in filepaths: + print("Looking in %s" % filepath) + cmd = ["sed", "-i", sed_string, filepath] + + stdout = '' + stderr = '' + try: + proc = subprocess.Popen(cmd, stdout=subprocess.PIPE, stderr=subprocess.PIPE) + stdout, stderr = proc.communicate() + if proc.returncode: + sys.stderr.write(stderr) + except: + sys.stderr.write(stderr) diff --git a/dbReports/iondb/bin/migrate_schema22.py b/dbReports/iondb/bin/migrate_schema22.py index 8d08aa22..44b85f49 100755 --- a/dbReports/iondb/bin/migrate_schema22.py +++ b/dbReports/iondb/bin/migrate_schema22.py @@ -998,56 +998,6 @@ def forwards(self, orm): log.info("UserProfile table already exists") db.clear_deferred_sql() - try: - db.start_transaction() - # Adding model 'SequencingKit' - db.create_table('rundb_sequencingkit', ( - ('id', models.fields.AutoField(primary_key=True)), - ('name', models.fields.CharField(max_length=512, blank=True)), - ('description', models.fields.CharField(max_length=3024, blank=True)), - ('sap', models.fields.CharField(max_length=7, blank=True)), - )) - db.send_create_signal('rundb', ['SequencingKit']) - db.execute_deferred_sql() - db.commit_transaction() - except DatabaseError: - db.rollback_transaction() - log.info("SequencingKit table already exists") - db.clear_deferred_sql() - - try: - db.start_transaction() - # Adding model 'LibraryKit' - db.create_table('rundb_librarykit', ( - ('id', models.fields.AutoField(primary_key=True)), - ('name', models.fields.CharField(max_length=512, blank=True)), - ('description', models.fields.CharField(max_length=3024, blank=True)), - ('sap', models.fields.CharField(max_length=7, blank=True)), - )) - db.send_create_signal('rundb', ['LibraryKit']) - db.execute_deferred_sql() - db.commit_transaction() - except DatabaseError: - db.rollback_transaction() - log.info("LibraryKit table already exists") - db.clear_deferred_sql() - - try: - db.start_transaction() - # Adding model 'VariantFrequencies' - db.create_table('rundb_variantfrequencies', ( - ('id', models.fields.AutoField(primary_key=True)), - ('name', models.fields.CharField(max_length=512, blank=True)), - ('description', models.fields.CharField(max_length=3024, blank=True)), - )) - db.send_create_signal('rundb', ['VariantFrequencies']) - db.execute_deferred_sql() - db.commit_transaction() - except DatabaseError: - db.rollback_transaction() - log.info("VariantFrequencies table already exists") - db.clear_deferred_sql() - try: db.start_transaction() # Adding model 'KitInfo' @@ -1619,13 +1569,6 @@ def backwards(self): 'name': ('django.db.models.fields.CharField', [], {'unique': 'True', 'max_length': '256'}), 'sequence': ('django.db.models.fields.CharField', [], {'max_length': '64'}) }, - 'rundb.librarykit': { - 'Meta': {'object_name': 'LibraryKit'}, - 'description': ('django.db.models.fields.CharField', [], {'max_length': '3024', 'blank': 'True'}), - 'id': ('django.db.models.fields.AutoField', [], {'primary_key': 'True'}), - 'name': ('django.db.models.fields.CharField', [], {'max_length': '512', 'blank': 'True'}), - 'sap': ('django.db.models.fields.CharField', [], {'max_length': '7', 'blank': 'True'}) - }, 'rundb.location': { 'Meta': {'object_name': 'Location'}, 'comments': ('django.db.models.fields.TextField', [], {'blank': 'True'}), @@ -1832,13 +1775,6 @@ def backwards(self): 'meta': ('django.db.models.fields.TextField', [], {'default': "''", 'null': 'True', 'blank': 'True'}), 'runType': ('django.db.models.fields.CharField', [], {'max_length': '512'}) }, - 'rundb.sequencingkit': { - 'Meta': {'object_name': 'SequencingKit'}, - 'description': ('django.db.models.fields.CharField', [], {'max_length': '3024', 'blank': 'True'}), - 'id': ('django.db.models.fields.AutoField', [], {'primary_key': 'True'}), - 'name': ('django.db.models.fields.CharField', [], {'max_length': '512', 'blank': 'True'}), - 'sap': ('django.db.models.fields.CharField', [], {'max_length': '7', 'blank': 'True'}) - }, 'rundb.template': { 'Meta': {'object_name': 'Template'}, 'comments': ('django.db.models.fields.TextField', [], {'blank': 'True'}), @@ -1914,12 +1850,6 @@ def backwards(self): 'phone_number': ('django.db.models.fields.CharField', [], {'default': "''", 'max_length': '256', 'blank': 'True'}), 'title': ('django.db.models.fields.CharField', [], {'default': "'user'", 'max_length': '256'}), 'user': ('django.db.models.fields.related.ForeignKey', [], {'to': "orm['auth.User']", 'unique': 'True'}) - }, - 'rundb.variantfrequencies': { - 'Meta': {'object_name': 'VariantFrequencies'}, - 'description': ('django.db.models.fields.CharField', [], {'max_length': '3024', 'blank': 'True'}), - 'id': ('django.db.models.fields.AutoField', [], {'primary_key': 'True'}), - 'name': ('django.db.models.fields.CharField', [], {'max_length': '512', 'blank': 'True'}) } } diff --git a/dbReports/iondb/bin/startup_housekeeping.py b/dbReports/iondb/bin/startup_housekeeping.py index 82e49bc0..b987a260 100644 --- a/dbReports/iondb/bin/startup_housekeeping.py +++ b/dbReports/iondb/bin/startup_housekeeping.py @@ -31,7 +31,7 @@ def bother_the_user(): info.extend([profile.phone_number, profile.user.email]) if not any(info): models.Message.info("""Please supply some customer support contact info. -
Add contact information.""") +
Add contact information.""") def events_from_settings(): diff --git a/dbReports/iondb/celery.py b/dbReports/iondb/celery.py index b0d03229..6ed17b73 100644 --- a/dbReports/iondb/celery.py +++ b/dbReports/iondb/celery.py @@ -24,7 +24,7 @@ CELERY_RESULT_BACKEND='cache+memcached://127.0.0.1:11211/', # Avoid indefinite hangs by forcing results to expire after 30 minutes - CELERY_TASK_RESULT_EXPIRES=180, + CELERY_TASK_RESULT_EXPIRES=1800, # Tracking rate limits is expensive, not used CELERY_DISABLE_RATE_LIMITS=True, diff --git a/dbReports/iondb/ftpserver/__init__.py b/dbReports/iondb/ftpserver/__init__.py new file mode 100644 index 00000000..bda83ac8 --- /dev/null +++ b/dbReports/iondb/ftpserver/__init__.py @@ -0,0 +1,7 @@ +#from iondb.ftpserver.management.commands.ftpserver import Command +#def start_ftp_server(): +# ftp_server_cmd = Command() + # call this to start the ftp server with all of the settings... from the settings +# ftp_server_cmd.handle() + +#start_ftp_server() diff --git a/dbReports/iondb/ftpserver/_unix.py b/dbReports/iondb/ftpserver/_unix.py new file mode 100644 index 00000000..5a6e3917 --- /dev/null +++ b/dbReports/iondb/ftpserver/_unix.py @@ -0,0 +1,25 @@ +try: + import os + import pwd +except ImportError: + pass +else: + class UnixPersonateUser(object): + def __init__(self, file_access_user): + self.file_access_user = file_access_user + self.gid = os.getgid() + self.uid = os.getuid() + + def impersonate_user(self, username, password): + """impersonate user when operating file system + """ + uid = pwd.getpwnam(self.file_access_user).pw_uid + gid = pwd.getpwnam(self.file_access_user).pw_gid + os.setegid(gid) + os.seteuid(uid) + + def terminate_impersonation(self, username): + """undo user from impersonation + """ + os.setegid(self.gid) + os.seteuid(self.uid) diff --git a/dbReports/iondb/ftpserver/authorizers.py b/dbReports/iondb/ftpserver/authorizers.py new file mode 100644 index 00000000..71acf452 --- /dev/null +++ b/dbReports/iondb/ftpserver/authorizers.py @@ -0,0 +1,63 @@ +import os +from django.contrib.auth import authenticate +from django.contrib.auth.models import User +from pyftpdlib.authorizers import AuthenticationFailed +from . import _unix + +PERMMISSIONS = 'elradfmw' + +class FTPAccountAuthorizer(object): + """Authorizer class by django authentication.""" + personate_user_class = None + + def __init__(self, file_access_user=None): + if file_access_user: + personate_user_class = (self.personate_user_class or _unix.UnixPersonateUser) + self.personate_user = personate_user_class(file_access_user) + else: + self.personate_user = None + + def has_user(self, username): + """return True if exists user.""" + return User.objects.filter(username=username).exists() + + def validate_authentication(self, username, password, handler): + """authenticate user with password""" + + if not authenticate(username=username, password=password): + raise AuthenticationFailed("Authentication failed.") + + try: + return User.objects.get(username=username) + except User.DoesNotExist: + raise AuthenticationFailed("Authentication failed.") + + def get_home_dir(self, username): + """Get the home directory""" + return '/' + + def get_msg_login(self, username): + """message for welcome.""" + return 'welcome.' + + def get_msg_quit(self, username): + """The quit message""" + return 'good bye.' + + def has_perm(self, username, perm, path=None): + """check user permission""" + return perm in PERMMISSIONS + + def get_perms(self, username): + """return user permissions""" + return PERMMISSIONS + + def impersonate_user(self, username, password): + """delegate to personate_user method""" + if self.personate_user: + self.personate_user.impersonate_user(username, password) + + def terminate_impersonation(self, username): + """delegate to terminate_impersonation method""" + if self.personate_user: + self.personate_user.terminate_impersonation(username) diff --git a/pipeline/tests/__init__.py b/dbReports/iondb/ftpserver/management/__init__.py similarity index 100% rename from pipeline/tests/__init__.py rename to dbReports/iondb/ftpserver/management/__init__.py diff --git a/dbReports/iondb/ftpserver/management/commands/__init__.py b/dbReports/iondb/ftpserver/management/commands/__init__.py new file mode 100644 index 00000000..e69de29b diff --git a/dbReports/iondb/ftpserver/management/commands/ftpserver.py b/dbReports/iondb/ftpserver/management/commands/ftpserver.py new file mode 100644 index 00000000..759f4f5c --- /dev/null +++ b/dbReports/iondb/ftpserver/management/commands/ftpserver.py @@ -0,0 +1,122 @@ +import sys +import os + +from optparse import make_option + +import pyftpdlib +from pyftpdlib import handlers +from pyftpdlib.servers import FTPServer + +from django import get_version +from django.conf import settings +from django.core.management.base import BaseCommand, CommandError +from django.utils.daemonize import become_daemon +from iondb.ftpserver.authorizers import FTPAccountAuthorizer +from iondb.ftpserver import utils + + +class Command(BaseCommand): + help = "Start FTP server" + + option_list = BaseCommand.option_list + ( + make_option('--daemonize', action='store_true', dest='daemonize', help="become background service."), + make_option('--pidfile', action='store', dest='pidfile', help="filename to write process id (PID)."), + make_option('--timeout', action='store', dest='timeout', type=int, help="timeout for remote client."), + make_option('--passive-ports', action='store', dest='passive-ports', help="Passive ports. eg. 12345,30000-50000"), + make_option('--masquerade-address', action='store', dest='masquerade-address', help="masquerade address."), + make_option('--file-access-user', action='store', dest='file-access-user', help="user for access to file."), + make_option('--certfile', action='store', dest='certfile', help="TLS certificate file."), + make_option('--keyfile', action='store', dest='keyfile', help="TLS private key file."), + make_option('--sendfile', action='store_true', dest='sendfile', help="Use sendfile."), + ) + + def make_server(self, server_class, handler_class, authorizer_class, host_port, file_access_user=None, **handler_options): + """Helper method for making a server""" + return utils.make_server(server_class, handler_class, authorizer_class, host_port, file_access_user=file_access_user, **handler_options) + + def handle(self, *args, **options): + # bind host and port + host_port = options.get('host_port', None) + if host_port: + host, _port = host_port.split(':', 1) + port = int(_port) + else: + host = utils.get_settings_value('FTPSERVER_HOST') or '127.0.0.1' + port = utils.get_settings_value('FTPSERVER_PORT') or 21 + + timeout = options.get('timeout') or utils.get_settings_value('FTPSERVER_TIMEOUT') + + # passive ports + _passive_ports = options.get('passive-ports') or utils.get_settings_value('FTPSERVER_PASSIVE_PORTS') + if _passive_ports: + try: + passive_ports = utils.parse_ports(_passive_ports) + except (TypeError, ValueError): + raise CommandError("Invalid passive ports: {}".format(_passive_ports)) + else: + passive_ports = None + + # masquerade address + masquerade_address = options.get('masquerade-address') or utils.get_settings_value('FTPSERVER_MASQUERADE_ADDRESS') + + # file access user + file_access_user = options.get('file-access-user') or utils.get_settings_value('FTPSERVER_FILE_ACCESS_USER') + + # certfile + certfile = options.get('certfile') or utils.get_settings_value('FTPSERVER_CERTFILE') + + # keyfile + keyfile = options.get('keyfile') or utils.get_settings_value('FTPSERVER_KEYFILE') + + # sendfile + sendfile = options.get('sendfile') or utils.get_settings_value('FTPSERVER_SENDFILE') + + # daemonize + daemonize = options.get('daemonize') or utils.get_settings_value('FTPSERVER_DAEMONIZE') + if daemonize: + daemonize_options = utils.get_settings_value('FTPSERVER_DAEMONIZE_OPTIONS') or {} + become_daemon(**daemonize_options) + + # write pid to file + pidfile = options.get('pidfile') or utils.get_settings_value('FTPSERVER_PIDFILE') + if pidfile: + with open(pidfile, 'w') as f: + f.write(str(os.getpid())) + + # select handler class + if certfile or keyfile: + if hasattr(handlers, 'TLS_FTPHandler'): + handler_class = (utils.get_settings_value('FTPSERVER_TLSHANDLER')) or handlers.TLS_FTPHandler + else: + # unsupported + raise CommandError("Can't import OpenSSL. Please install pyOpenSSL.") + else: + handler_class = (utils.get_settings_value('FTPSERVER_HANDLER')) or handlers.FTPHandler + + # setup server + server = self.make_server( + server_class=FTPServer, + handler_class=handler_class, + authorizer_class=FTPAccountAuthorizer, + host_port=(host, port), + file_access_user=file_access_user, + timeout=timeout, + passive_ports=passive_ports, + masquerade_address=masquerade_address, + certfile=certfile, + keyfile=keyfile, + sendfile=sendfile + ) + + # start server + sys.stdout.write(( + "Django version {version_dj}, using settings '{settings}'\n" + "pyftpdlib version {version_ftp}\n" + "Quit the server with {quit_command}.\n").format( + version_dj=get_version(), + version_ftp=pyftpdlib.__ver__, + settings=settings.SETTINGS_MODULE, + quit_command='CONTROL-C' + ) + ) + server.serve_forever() diff --git a/dbReports/iondb/ftpserver/utils.py b/dbReports/iondb/ftpserver/utils.py new file mode 100644 index 00000000..29622323 --- /dev/null +++ b/dbReports/iondb/ftpserver/utils.py @@ -0,0 +1,59 @@ +from django.conf import settings + + +def get_settings_value(name): + """Return the django settings value for name attribute + """ + return getattr(settings, name, None) + + +def parse_ports(ports_text): + """Parse ports text + + e.g. ports_text = "12345,13000-15000,20000-30000" + """ + ports_set = set() + for bit in ports_text.split(','): + if '-' in bit: + low, high = bit.split('-', 1) + ports_set = ports_set.union(range(int(low), int(high) + 1)) + else: + ports_set.add(int(bit)) + return sorted(list(ports_set)) + + +def import_class(class_path): + from importlib import import_module + pieces = class_path.split('.') + module = '.'.join(pieces[:-1]) + cls = pieces[-1] + module = import_module(module) + return getattr(module, cls) + + +def make_server(server_class, handler_class, authorizer_class, host_port, file_access_user=None, **handler_options): + """make server instance + + :host_port: (host, port) + :file_access_user: 'spam' + + handler_options: + + * timeout + * passive_ports + * masquerade_address + * certfile + * keyfile + """ + if isinstance(handler_class, basestring): + handler_class = import_class(handler_class) + + if isinstance(authorizer_class, basestring): + authorizer_class = import_class(authorizer_class) + + authorizer = authorizer_class(file_access_user) + handler = handler_class + for key, value in handler_options.items(): + setattr(handler, key, value) + handler.authorizer = authorizer + return server_class(host_port, handler) diff --git a/dbReports/iondb/media/Makefile b/dbReports/iondb/media/Makefile index afe3cf87..24859fab 100644 --- a/dbReports/iondb/media/Makefile +++ b/dbReports/iondb/media/Makefile @@ -32,7 +32,7 @@ hr=-------------------------------------------------- # # BUILD DOCS # -all: |compile-css minify-js +all: |compile-css #minify-js compile-css: @echo "\n${HR}" @@ -112,7 +112,6 @@ minify-js: | bulk-minify-js @echo "Minify'ing random Torrent Browser JS" @uglifyjs -nc runliveness.js > runliveness.min.js @uglifyjs -nc tbHelper.js > tbHelper.min.js - @uglifyjs -nc resources/jquery/jquery.iframe-auto-height.plugin.1.7.1.js > resources/jquery/jquery.iframe-auto-height.plugin.1.7.1.min.js @echo "Minify'ing random Torrent Browser JS... ${CHECK} Done" @echo "${HR}" diff --git a/dbReports/iondb/media/jquery/js/plupload/jquery-ui.min.js b/dbReports/iondb/media/jquery/js/plupload/jquery-ui.min.js deleted file mode 100644 index c11e844f..00000000 --- a/dbReports/iondb/media/jquery/js/plupload/jquery-ui.min.js +++ /dev/null @@ -1,1012 +0,0 @@ -/*! - * jQuery UI 1.8.2 - * - * Copyright (c) 2010 AUTHORS.txt (http://jqueryui.com/about) - * Dual licensed under the MIT (MIT-LICENSE.txt) - * and GPL (GPL-LICENSE.txt) licenses. - * - * http://docs.jquery.com/UI - */ -(function(c){c.ui=c.ui||{};if(!c.ui.version){c.extend(c.ui,{version:"1.8.2",plugin:{add:function(a,b,d){a=c.ui[a].prototype;for(var e in 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e=b.my[1]==="top"?-b.elemHeight:b.my[1]==="bottom"?b.elemHeight:0,g=b.at[1]==="top"?b.targetHeight:-b.targetHeight,h=-2*b.offset[1];a.top+=a.top<0?e+b.targetHeight+h:d>0?e+g+h:0}}}};if(!c.offset.setOffset){c.offset.setOffset=function(a,b){if(/static/.test(c.curCSS(a,"position")))a.style.position= -"relative";var d=c(a),e=d.offset(),g=parseInt(c.curCSS(a,"top",true),10)||0,h=parseInt(c.curCSS(a,"left",true),10)||0;e={top:b.top-e.top+g,left:b.left-e.left+h};"using"in b?b.using.call(a,e):d.css(e)};c.fn.offset=function(a){var b=this[0];if(!b||!b.ownerDocument)return null;if(a)return this.each(function(){c.offset.setOffset(this,a)});return q.call(this)}}})(jQuery); -;/* - * jQuery UI Draggable 1.8.2 - * - * Copyright (c) 2010 AUTHORS.txt (http://jqueryui.com/about) - * Dual licensed under the MIT (MIT-LICENSE.txt) - * and GPL (GPL-LICENSE.txt) licenses. - * - * http://docs.jquery.com/UI/Draggables - * - * Depends: - * jquery.ui.core.js - * jquery.ui.mouse.js - * jquery.ui.widget.js - */ -(function(d){d.widget("ui.draggable",d.ui.mouse,{widgetEventPrefix:"drag",options:{addClasses:true,appendTo:"parent",axis:false,connectToSortable:false,containment:false,cursor:"auto",cursorAt:false,grid:false,handle:false,helper:"original",iframeFix:false,opacity:false,refreshPositions:false,revert:false,revertDuration:500,scope:"default",scroll:true,scrollSensitivity:20,scrollSpeed:20,snap:false,snapMode:"both",snapTolerance:20,stack:false,zIndex:false},_create:function(){if(this.options.helper== -"original"&&!/^(?:r|a|f)/.test(this.element.css("position")))this.element[0].style.position="relative";this.options.addClasses&&this.element.addClass("ui-draggable");this.options.disabled&&this.element.addClass("ui-draggable-disabled");this._mouseInit()},destroy:function(){if(this.element.data("draggable")){this.element.removeData("draggable").unbind(".draggable").removeClass("ui-draggable ui-draggable-dragging ui-draggable-disabled");this._mouseDestroy();return this}},_mouseCapture:function(a){var b= -this.options;if(this.helper||b.disabled||d(a.target).is(".ui-resizable-handle"))return false;this.handle=this._getHandle(a);if(!this.handle)return false;return true},_mouseStart:function(a){var b=this.options;this.helper=this._createHelper(a);this._cacheHelperProportions();if(d.ui.ddmanager)d.ui.ddmanager.current=this;this._cacheMargins();this.cssPosition=this.helper.css("position");this.scrollParent=this.helper.scrollParent();this.offset=this.positionAbs=this.element.offset();this.offset={top:this.offset.top- -this.margins.top,left:this.offset.left-this.margins.left};d.extend(this.offset,{click:{left:a.pageX-this.offset.left,top:a.pageY-this.offset.top},parent:this._getParentOffset(),relative:this._getRelativeOffset()});this.originalPosition=this.position=this._generatePosition(a);this.originalPageX=a.pageX;this.originalPageY=a.pageY;b.cursorAt&&this._adjustOffsetFromHelper(b.cursorAt);b.containment&&this._setContainment();if(this._trigger("start",a)===false){this._clear();return false}this._cacheHelperProportions(); -d.ui.ddmanager&&!b.dropBehaviour&&d.ui.ddmanager.prepareOffsets(this,a);this.helper.addClass("ui-draggable-dragging");this._mouseDrag(a,true);return true},_mouseDrag:function(a,b){this.position=this._generatePosition(a);this.positionAbs=this._convertPositionTo("absolute");if(!b){b=this._uiHash();if(this._trigger("drag",a,b)===false){this._mouseUp({});return false}this.position=b.position}if(!this.options.axis||this.options.axis!="y")this.helper[0].style.left=this.position.left+"px";if(!this.options.axis|| -this.options.axis!="x")this.helper[0].style.top=this.position.top+"px";d.ui.ddmanager&&d.ui.ddmanager.drag(this,a);return false},_mouseStop:function(a){var b=false;if(d.ui.ddmanager&&!this.options.dropBehaviour)b=d.ui.ddmanager.drop(this,a);if(this.dropped){b=this.dropped;this.dropped=false}if(!this.element[0]||!this.element[0].parentNode)return false;if(this.options.revert=="invalid"&&!b||this.options.revert=="valid"&&b||this.options.revert===true||d.isFunction(this.options.revert)&&this.options.revert.call(this.element, -b)){var c=this;d(this.helper).animate(this.originalPosition,parseInt(this.options.revertDuration,10),function(){c._trigger("stop",a)!==false&&c._clear()})}else this._trigger("stop",a)!==false&&this._clear();return false},cancel:function(){this.helper.is(".ui-draggable-dragging")?this._mouseUp({}):this._clear();return this},_getHandle:function(a){var b=!this.options.handle||!d(this.options.handle,this.element).length?true:false;d(this.options.handle,this.element).find("*").andSelf().each(function(){if(this== -a.target)b=true});return b},_createHelper:function(a){var b=this.options;a=d.isFunction(b.helper)?d(b.helper.apply(this.element[0],[a])):b.helper=="clone"?this.element.clone():this.element;a.parents("body").length||a.appendTo(b.appendTo=="parent"?this.element[0].parentNode:b.appendTo);a[0]!=this.element[0]&&!/(fixed|absolute)/.test(a.css("position"))&&a.css("position","absolute");return a},_adjustOffsetFromHelper:function(a){if(typeof a=="string")a=a.split(" ");if(d.isArray(a))a={left:+a[0],top:+a[1]|| -0};if("left"in a)this.offset.click.left=a.left+this.margins.left;if("right"in a)this.offset.click.left=this.helperProportions.width-a.right+this.margins.left;if("top"in a)this.offset.click.top=a.top+this.margins.top;if("bottom"in a)this.offset.click.top=this.helperProportions.height-a.bottom+this.margins.top},_getParentOffset:function(){this.offsetParent=this.helper.offsetParent();var a=this.offsetParent.offset();if(this.cssPosition=="absolute"&&this.scrollParent[0]!=document&&d.ui.contains(this.scrollParent[0], -this.offsetParent[0])){a.left+=this.scrollParent.scrollLeft();a.top+=this.scrollParent.scrollTop()}if(this.offsetParent[0]==document.body||this.offsetParent[0].tagName&&this.offsetParent[0].tagName.toLowerCase()=="html"&&d.browser.msie)a={top:0,left:0};return{top:a.top+(parseInt(this.offsetParent.css("borderTopWidth"),10)||0),left:a.left+(parseInt(this.offsetParent.css("borderLeftWidth"),10)||0)}},_getRelativeOffset:function(){if(this.cssPosition=="relative"){var a=this.element.position();return{top:a.top- -(parseInt(this.helper.css("top"),10)||0)+this.scrollParent.scrollTop(),left:a.left-(parseInt(this.helper.css("left"),10)||0)+this.scrollParent.scrollLeft()}}else return{top:0,left:0}},_cacheMargins:function(){this.margins={left:parseInt(this.element.css("marginLeft"),10)||0,top:parseInt(this.element.css("marginTop"),10)||0}},_cacheHelperProportions:function(){this.helperProportions={width:this.helper.outerWidth(),height:this.helper.outerHeight()}},_setContainment:function(){var a=this.options;if(a.containment== -"parent")a.containment=this.helper[0].parentNode;if(a.containment=="document"||a.containment=="window")this.containment=[0-this.offset.relative.left-this.offset.parent.left,0-this.offset.relative.top-this.offset.parent.top,d(a.containment=="document"?document:window).width()-this.helperProportions.width-this.margins.left,(d(a.containment=="document"?document:window).height()||document.body.parentNode.scrollHeight)-this.helperProportions.height-this.margins.top];if(!/^(document|window|parent)$/.test(a.containment)&& -a.containment.constructor!=Array){var b=d(a.containment)[0];if(b){a=d(a.containment).offset();var c=d(b).css("overflow")!="hidden";this.containment=[a.left+(parseInt(d(b).css("borderLeftWidth"),10)||0)+(parseInt(d(b).css("paddingLeft"),10)||0)-this.margins.left,a.top+(parseInt(d(b).css("borderTopWidth"),10)||0)+(parseInt(d(b).css("paddingTop"),10)||0)-this.margins.top,a.left+(c?Math.max(b.scrollWidth,b.offsetWidth):b.offsetWidth)-(parseInt(d(b).css("borderLeftWidth"),10)||0)-(parseInt(d(b).css("paddingRight"), -10)||0)-this.helperProportions.width-this.margins.left,a.top+(c?Math.max(b.scrollHeight,b.offsetHeight):b.offsetHeight)-(parseInt(d(b).css("borderTopWidth"),10)||0)-(parseInt(d(b).css("paddingBottom"),10)||0)-this.helperProportions.height-this.margins.top]}}else if(a.containment.constructor==Array)this.containment=a.containment},_convertPositionTo:function(a,b){if(!b)b=this.position;a=a=="absolute"?1:-1;var c=this.cssPosition=="absolute"&&!(this.scrollParent[0]!=document&&d.ui.contains(this.scrollParent[0], -this.offsetParent[0]))?this.offsetParent:this.scrollParent,f=/(html|body)/i.test(c[0].tagName);return{top:b.top+this.offset.relative.top*a+this.offset.parent.top*a-(d.browser.safari&&d.browser.version<526&&this.cssPosition=="fixed"?0:(this.cssPosition=="fixed"?-this.scrollParent.scrollTop():f?0:c.scrollTop())*a),left:b.left+this.offset.relative.left*a+this.offset.parent.left*a-(d.browser.safari&&d.browser.version<526&&this.cssPosition=="fixed"?0:(this.cssPosition=="fixed"?-this.scrollParent.scrollLeft(): -f?0:c.scrollLeft())*a)}},_generatePosition:function(a){var b=this.options,c=this.cssPosition=="absolute"&&!(this.scrollParent[0]!=document&&d.ui.contains(this.scrollParent[0],this.offsetParent[0]))?this.offsetParent:this.scrollParent,f=/(html|body)/i.test(c[0].tagName),e=a.pageX,g=a.pageY;if(this.originalPosition){if(this.containment){if(a.pageX-this.offset.click.leftthis.containment[2])e=this.containment[2]+this.offset.click.left;if(a.pageY-this.offset.click.top>this.containment[3])g=this.containment[3]+this.offset.click.top}if(b.grid){g=this.originalPageY+Math.round((g-this.originalPageY)/b.grid[1])*b.grid[1];g=this.containment?!(g-this.offset.click.topthis.containment[3])?g:!(g-this.offset.click.topthis.containment[2])?e:!(e-this.offset.click.left').css({width:this.offsetWidth+"px",height:this.offsetHeight+"px",position:"absolute",opacity:"0.001",zIndex:1E3}).css(d(this).offset()).appendTo("body")})},stop:function(){d("div.ui-draggable-iframeFix").each(function(){this.parentNode.removeChild(this)})}});d.ui.plugin.add("draggable","opacity",{start:function(a,b){a=d(b.helper);b=d(this).data("draggable").options; -if(a.css("opacity"))b._opacity=a.css("opacity");a.css("opacity",b.opacity)},stop:function(a,b){a=d(this).data("draggable").options;a._opacity&&d(b.helper).css("opacity",a._opacity)}});d.ui.plugin.add("draggable","scroll",{start:function(){var a=d(this).data("draggable");if(a.scrollParent[0]!=document&&a.scrollParent[0].tagName!="HTML")a.overflowOffset=a.scrollParent.offset()},drag:function(a){var b=d(this).data("draggable"),c=b.options,f=false;if(b.scrollParent[0]!=document&&b.scrollParent[0].tagName!= -"HTML"){if(!c.axis||c.axis!="x")if(b.overflowOffset.top+b.scrollParent[0].offsetHeight-a.pageY=0;h--){var i=c.snapElements[h].left,k=i+c.snapElements[h].width,j=c.snapElements[h].top,l=j+c.snapElements[h].height;if(i-e=j&&f<=l||h>=j&&h<=l||fl)&&(e>=i&& -e<=k||g>=i&&g<=k||ek);default:return false}};d.ui.ddmanager={current:null,droppables:{"default":[]},prepareOffsets:function(a,b){var c=d.ui.ddmanager.droppables[a.options.scope]||[],e=b?b.type:null,g=(a.currentItem||a.element).find(":data(droppable)").andSelf(),f=0;a:for(;f').css({position:this.element.css("position"),width:this.element.outerWidth(),height:this.element.outerHeight(), -top:this.element.css("top"),left:this.element.css("left")}));this.element=this.element.parent().data("resizable",this.element.data("resizable"));this.elementIsWrapper=true;this.element.css({marginLeft:this.originalElement.css("marginLeft"),marginTop:this.originalElement.css("marginTop"),marginRight:this.originalElement.css("marginRight"),marginBottom:this.originalElement.css("marginBottom")});this.originalElement.css({marginLeft:0,marginTop:0,marginRight:0,marginBottom:0});this.originalResizeStyle= -this.originalElement.css("resize");this.originalElement.css("resize","none");this._proportionallyResizeElements.push(this.originalElement.css({position:"static",zoom:1,display:"block"}));this.originalElement.css({margin:this.originalElement.css("margin")});this._proportionallyResize()}this.handles=a.handles||(!d(".ui-resizable-handle",this.element).length?"e,s,se":{n:".ui-resizable-n",e:".ui-resizable-e",s:".ui-resizable-s",w:".ui-resizable-w",se:".ui-resizable-se",sw:".ui-resizable-sw",ne:".ui-resizable-ne", -nw:".ui-resizable-nw"});if(this.handles.constructor==String){if(this.handles=="all")this.handles="n,e,s,w,se,sw,ne,nw";var c=this.handles.split(",");this.handles={};for(var e=0;e');/sw|se|ne|nw/.test(g)&&f.css({zIndex:++a.zIndex});"se"==g&&f.addClass("ui-icon ui-icon-gripsmall-diagonal-se");this.handles[g]=".ui-resizable-"+g;this.element.append(f)}}this._renderAxis=function(h){h=h||this.element;for(var i in this.handles){if(this.handles[i].constructor== -String)this.handles[i]=d(this.handles[i],this.element).show();if(this.elementIsWrapper&&this.originalElement[0].nodeName.match(/textarea|input|select|button/i)){var j=d(this.handles[i],this.element),l=0;l=/sw|ne|nw|se|n|s/.test(i)?j.outerHeight():j.outerWidth();j=["padding",/ne|nw|n/.test(i)?"Top":/se|sw|s/.test(i)?"Bottom":/^e$/.test(i)?"Right":"Left"].join("");h.css(j,l);this._proportionallyResize()}d(this.handles[i])}};this._renderAxis(this.element);this._handles=d(".ui-resizable-handle",this.element).disableSelection(); -this._handles.mouseover(function(){if(!b.resizing){if(this.className)var h=this.className.match(/ui-resizable-(se|sw|ne|nw|n|e|s|w)/i);b.axis=h&&h[1]?h[1]:"se"}});if(a.autoHide){this._handles.hide();d(this.element).addClass("ui-resizable-autohide").hover(function(){d(this).removeClass("ui-resizable-autohide");b._handles.show()},function(){if(!b.resizing){d(this).addClass("ui-resizable-autohide");b._handles.hide()}})}this._mouseInit()},destroy:function(){this._mouseDestroy();var b=function(c){d(c).removeClass("ui-resizable ui-resizable-disabled ui-resizable-resizing").removeData("resizable").unbind(".resizable").find(".ui-resizable-handle").remove()}; -if(this.elementIsWrapper){b(this.element);var a=this.element;a.after(this.originalElement.css({position:a.css("position"),width:a.outerWidth(),height:a.outerHeight(),top:a.css("top"),left:a.css("left")})).remove()}this.originalElement.css("resize",this.originalResizeStyle);b(this.originalElement);return this},_mouseCapture:function(b){var a=false;for(var c in this.handles)if(d(this.handles[c])[0]==b.target)a=true;return!this.options.disabled&&a},_mouseStart:function(b){var a=this.options,c=this.element.position(), -e=this.element;this.resizing=true;this.documentScroll={top:d(document).scrollTop(),left:d(document).scrollLeft()};if(e.is(".ui-draggable")||/absolute/.test(e.css("position")))e.css({position:"absolute",top:c.top,left:c.left});d.browser.opera&&/relative/.test(e.css("position"))&&e.css({position:"relative",top:"auto",left:"auto"});this._renderProxy();c=m(this.helper.css("left"));var g=m(this.helper.css("top"));if(a.containment){c+=d(a.containment).scrollLeft()||0;g+=d(a.containment).scrollTop()||0}this.offset= -this.helper.offset();this.position={left:c,top:g};this.size=this._helper?{width:e.outerWidth(),height:e.outerHeight()}:{width:e.width(),height:e.height()};this.originalSize=this._helper?{width:e.outerWidth(),height:e.outerHeight()}:{width:e.width(),height:e.height()};this.originalPosition={left:c,top:g};this.sizeDiff={width:e.outerWidth()-e.width(),height:e.outerHeight()-e.height()};this.originalMousePosition={left:b.pageX,top:b.pageY};this.aspectRatio=typeof a.aspectRatio=="number"?a.aspectRatio: -this.originalSize.width/this.originalSize.height||1;a=d(".ui-resizable-"+this.axis).css("cursor");d("body").css("cursor",a=="auto"?this.axis+"-resize":a);e.addClass("ui-resizable-resizing");this._propagate("start",b);return true},_mouseDrag:function(b){var a=this.helper,c=this.originalMousePosition,e=this._change[this.axis];if(!e)return false;c=e.apply(this,[b,b.pageX-c.left||0,b.pageY-c.top||0]);if(this._aspectRatio||b.shiftKey)c=this._updateRatio(c,b);c=this._respectSize(c,b);this._propagate("resize", -b);a.css({top:this.position.top+"px",left:this.position.left+"px",width:this.size.width+"px",height:this.size.height+"px"});!this._helper&&this._proportionallyResizeElements.length&&this._proportionallyResize();this._updateCache(c);this._trigger("resize",b,this.ui());return false},_mouseStop:function(b){this.resizing=false;var a=this.options,c=this;if(this._helper){var e=this._proportionallyResizeElements,g=e.length&&/textarea/i.test(e[0].nodeName);e=g&&d.ui.hasScroll(e[0],"left")?0:c.sizeDiff.height; -g={width:c.size.width-(g?0:c.sizeDiff.width),height:c.size.height-e};e=parseInt(c.element.css("left"),10)+(c.position.left-c.originalPosition.left)||null;var f=parseInt(c.element.css("top"),10)+(c.position.top-c.originalPosition.top)||null;a.animate||this.element.css(d.extend(g,{top:f,left:e}));c.helper.height(c.size.height);c.helper.width(c.size.width);this._helper&&!a.animate&&this._proportionallyResize()}d("body").css("cursor","auto");this.element.removeClass("ui-resizable-resizing");this._propagate("stop", -b);this._helper&&this.helper.remove();return false},_updateCache:function(b){this.offset=this.helper.offset();if(k(b.left))this.position.left=b.left;if(k(b.top))this.position.top=b.top;if(k(b.height))this.size.height=b.height;if(k(b.width))this.size.width=b.width},_updateRatio:function(b){var a=this.position,c=this.size,e=this.axis;if(b.height)b.width=c.height*this.aspectRatio;else if(b.width)b.height=c.width/this.aspectRatio;if(e=="sw"){b.left=a.left+(c.width-b.width);b.top=null}if(e=="nw"){b.top= -a.top+(c.height-b.height);b.left=a.left+(c.width-b.width)}return b},_respectSize:function(b){var a=this.options,c=this.axis,e=k(b.width)&&a.maxWidth&&a.maxWidthb.width,h=k(b.height)&&a.minHeight&&a.minHeight>b.height;if(f)b.width=a.minWidth;if(h)b.height=a.minHeight;if(e)b.width=a.maxWidth;if(g)b.height=a.maxHeight;var i=this.originalPosition.left+this.originalSize.width,j=this.position.top+this.size.height, -l=/sw|nw|w/.test(c);c=/nw|ne|n/.test(c);if(f&&l)b.left=i-a.minWidth;if(e&&l)b.left=i-a.maxWidth;if(h&&c)b.top=j-a.minHeight;if(g&&c)b.top=j-a.maxHeight;if((a=!b.width&&!b.height)&&!b.left&&b.top)b.top=null;else if(a&&!b.top&&b.left)b.left=null;return b},_proportionallyResize:function(){if(this._proportionallyResizeElements.length)for(var b=this.helper||this.element,a=0;a');var a=d.browser.msie&&d.browser.version<7,c=a?1:0;a=a?2:-1;this.helper.addClass(this._helper).css({width:this.element.outerWidth()+a,height:this.element.outerHeight()+a,position:"absolute",left:this.elementOffset.left-c+"px",top:this.elementOffset.top-c+"px",zIndex:++b.zIndex});this.helper.appendTo("body").disableSelection()}else this.helper=this.element},_change:{e:function(b,a){return{width:this.originalSize.width+ -a}},w:function(b,a){return{left:this.originalPosition.left+a,width:this.originalSize.width-a}},n:function(b,a,c){return{top:this.originalPosition.top+c,height:this.originalSize.height-c}},s:function(b,a,c){return{height:this.originalSize.height+c}},se:function(b,a,c){return d.extend(this._change.s.apply(this,arguments),this._change.e.apply(this,[b,a,c]))},sw:function(b,a,c){return d.extend(this._change.s.apply(this,arguments),this._change.w.apply(this,[b,a,c]))},ne:function(b,a,c){return d.extend(this._change.n.apply(this, -arguments),this._change.e.apply(this,[b,a,c]))},nw:function(b,a,c){return d.extend(this._change.n.apply(this,arguments),this._change.w.apply(this,[b,a,c]))}},_propagate:function(b,a){d.ui.plugin.call(this,b,[a,this.ui()]);b!="resize"&&this._trigger(b,a,this.ui())},plugins:{},ui:function(){return{originalElement:this.originalElement,element:this.element,helper:this.helper,position:this.position,size:this.size,originalSize:this.originalSize,originalPosition:this.originalPosition}}});d.extend(d.ui.resizable, -{version:"1.8.2"});d.ui.plugin.add("resizable","alsoResize",{start:function(){var b=d(this).data("resizable").options,a=function(c){d(c).each(function(){d(this).data("resizable-alsoresize",{width:parseInt(d(this).width(),10),height:parseInt(d(this).height(),10),left:parseInt(d(this).css("left"),10),top:parseInt(d(this).css("top"),10)})})};if(typeof b.alsoResize=="object"&&!b.alsoResize.parentNode)if(b.alsoResize.length){b.alsoResize=b.alsoResize[0];a(b.alsoResize)}else d.each(b.alsoResize,function(c){a(c)}); -else a(b.alsoResize)},resize:function(){var b=d(this).data("resizable"),a=b.options,c=b.originalSize,e=b.originalPosition,g={height:b.size.height-c.height||0,width:b.size.width-c.width||0,top:b.position.top-e.top||0,left:b.position.left-e.left||0},f=function(h,i){d(h).each(function(){var j=d(this),l=d(this).data("resizable-alsoresize"),p={};d.each((i&&i.length?i:["width","height","top","left"])||["width","height","top","left"],function(n,o){if((n=(l[o]||0)+(g[o]||0))&&n>=0)p[o]=n||null});if(/relative/.test(j.css("position"))&& -d.browser.opera){b._revertToRelativePosition=true;j.css({position:"absolute",top:"auto",left:"auto"})}j.css(p)})};typeof a.alsoResize=="object"&&!a.alsoResize.nodeType?d.each(a.alsoResize,function(h,i){f(h,i)}):f(a.alsoResize)},stop:function(){var b=d(this).data("resizable");if(b._revertToRelativePosition&&d.browser.opera){b._revertToRelativePosition=false;el.css({position:"relative"})}d(this).removeData("resizable-alsoresize-start")}});d.ui.plugin.add("resizable","animate",{stop:function(b){var a= -d(this).data("resizable"),c=a.options,e=a._proportionallyResizeElements,g=e.length&&/textarea/i.test(e[0].nodeName),f=g&&d.ui.hasScroll(e[0],"left")?0:a.sizeDiff.height;g={width:a.size.width-(g?0:a.sizeDiff.width),height:a.size.height-f};f=parseInt(a.element.css("left"),10)+(a.position.left-a.originalPosition.left)||null;var h=parseInt(a.element.css("top"),10)+(a.position.top-a.originalPosition.top)||null;a.element.animate(d.extend(g,h&&f?{top:h,left:f}:{}),{duration:c.animateDuration,easing:c.animateEasing, -step:function(){var i={width:parseInt(a.element.css("width"),10),height:parseInt(a.element.css("height"),10),top:parseInt(a.element.css("top"),10),left:parseInt(a.element.css("left"),10)};e&&e.length&&d(e[0]).css({width:i.width,height:i.height});a._updateCache(i);a._propagate("resize",b)}})}});d.ui.plugin.add("resizable","containment",{start:function(){var b=d(this).data("resizable"),a=b.element,c=b.options.containment;if(a=c instanceof d?c.get(0):/parent/.test(c)?a.parent().get(0):c){b.containerElement= -d(a);if(/document/.test(c)||c==document){b.containerOffset={left:0,top:0};b.containerPosition={left:0,top:0};b.parentData={element:d(document),left:0,top:0,width:d(document).width(),height:d(document).height()||document.body.parentNode.scrollHeight}}else{var e=d(a),g=[];d(["Top","Right","Left","Bottom"]).each(function(i,j){g[i]=m(e.css("padding"+j))});b.containerOffset=e.offset();b.containerPosition=e.position();b.containerSize={height:e.innerHeight()-g[3],width:e.innerWidth()-g[1]};c=b.containerOffset; -var f=b.containerSize.height,h=b.containerSize.width;h=d.ui.hasScroll(a,"left")?a.scrollWidth:h;f=d.ui.hasScroll(a)?a.scrollHeight:f;b.parentData={element:a,left:c.left,top:c.top,width:h,height:f}}}},resize:function(b){var a=d(this).data("resizable"),c=a.options,e=a.containerOffset,g=a.position;b=a._aspectRatio||b.shiftKey;var f={top:0,left:0},h=a.containerElement;if(h[0]!=document&&/static/.test(h.css("position")))f=e;if(g.left<(a._helper?e.left:0)){a.size.width+=a._helper?a.position.left-e.left: -a.position.left-f.left;if(b)a.size.height=a.size.width/c.aspectRatio;a.position.left=c.helper?e.left:0}if(g.top<(a._helper?e.top:0)){a.size.height+=a._helper?a.position.top-e.top:a.position.top;if(b)a.size.width=a.size.height*c.aspectRatio;a.position.top=a._helper?e.top:0}a.offset.left=a.parentData.left+a.position.left;a.offset.top=a.parentData.top+a.position.top;c=Math.abs((a._helper?a.offset.left-f.left:a.offset.left-f.left)+a.sizeDiff.width);e=Math.abs((a._helper?a.offset.top-f.top:a.offset.top- -e.top)+a.sizeDiff.height);g=a.containerElement.get(0)==a.element.parent().get(0);f=/relative|absolute/.test(a.containerElement.css("position"));if(g&&f)c-=a.parentData.left;if(c+a.size.width>=a.parentData.width){a.size.width=a.parentData.width-c;if(b)a.size.height=a.size.width/a.aspectRatio}if(e+a.size.height>=a.parentData.height){a.size.height=a.parentData.height-e;if(b)a.size.width=a.size.height*a.aspectRatio}},stop:function(){var b=d(this).data("resizable"),a=b.options,c=b.containerOffset,e=b.containerPosition, -g=b.containerElement,f=d(b.helper),h=f.offset(),i=f.outerWidth()-b.sizeDiff.width;f=f.outerHeight()-b.sizeDiff.height;b._helper&&!a.animate&&/relative/.test(g.css("position"))&&d(this).css({left:h.left-e.left-c.left,width:i,height:f});b._helper&&!a.animate&&/static/.test(g.css("position"))&&d(this).css({left:h.left-e.left-c.left,width:i,height:f})}});d.ui.plugin.add("resizable","ghost",{start:function(){var b=d(this).data("resizable"),a=b.options,c=b.size;b.ghost=b.originalElement.clone();b.ghost.css({opacity:0.25, -display:"block",position:"relative",height:c.height,width:c.width,margin:0,left:0,top:0}).addClass("ui-resizable-ghost").addClass(typeof a.ghost=="string"?a.ghost:"");b.ghost.appendTo(b.helper)},resize:function(){var b=d(this).data("resizable");b.ghost&&b.ghost.css({position:"relative",height:b.size.height,width:b.size.width})},stop:function(){var b=d(this).data("resizable");b.ghost&&b.helper&&b.helper.get(0).removeChild(b.ghost.get(0))}});d.ui.plugin.add("resizable","grid",{resize:function(){var b= -d(this).data("resizable"),a=b.options,c=b.size,e=b.originalSize,g=b.originalPosition,f=b.axis;a.grid=typeof a.grid=="number"?[a.grid,a.grid]:a.grid;var h=Math.round((c.width-e.width)/(a.grid[0]||1))*(a.grid[0]||1);a=Math.round((c.height-e.height)/(a.grid[1]||1))*(a.grid[1]||1);if(/^(se|s|e)$/.test(f)){b.size.width=e.width+h;b.size.height=e.height+a}else if(/^(ne)$/.test(f)){b.size.width=e.width+h;b.size.height=e.height+a;b.position.top=g.top-a}else{if(/^(sw)$/.test(f)){b.size.width=e.width+h;b.size.height= -e.height+a}else{b.size.width=e.width+h;b.size.height=e.height+a;b.position.top=g.top-a}b.position.left=g.left-h}}});var m=function(b){return parseInt(b,10)||0},k=function(b){return!isNaN(parseInt(b,10))}})(jQuery); -; -/* - * jQuery UI Selectable 1.8.2 - * - * Copyright (c) 2010 AUTHORS.txt (http://jqueryui.com/about) - * Dual licensed under the MIT (MIT-LICENSE.txt) - * and GPL (GPL-LICENSE.txt) licenses. - * - * http://docs.jquery.com/UI/Selectables - * - * Depends: - * jquery.ui.core.js - * jquery.ui.mouse.js - * jquery.ui.widget.js - */ -(function($) { - -$.widget("ui.selectable", $.ui.mouse, { - options: { - appendTo: 'body', - autoRefresh: true, - distance: 0, - filter: '*', - tolerance: 'touch' - }, - _create: function() { - var self = this; - - this.element.addClass("ui-selectable"); - - this.dragged = false; - - // cache selectee children based on filter - var selectees; - this.refresh = function() { - selectees = $(self.options.filter, self.element[0]); - selectees.each(function() { - var $this = $(this); - var pos = $this.offset(); - $.data(this, "selectable-item", { - element: this, - $element: $this, - left: pos.left, - top: pos.top, - right: pos.left + $this.outerWidth(), - bottom: pos.top + $this.outerHeight(), - startselected: false, - selected: $this.hasClass('ui-selected'), - selecting: $this.hasClass('ui-selecting'), - unselecting: $this.hasClass('ui-unselecting') - }); - }); - }; - this.refresh(); - - this.selectees = selectees.addClass("ui-selectee"); - - this._mouseInit(); - - this.helper = $("

"); - }, - - destroy: function() { - this.selectees - .removeClass("ui-selectee") - .removeData("selectable-item"); - this.element - .removeClass("ui-selectable ui-selectable-disabled") - .removeData("selectable") - .unbind(".selectable"); - this._mouseDestroy(); - - return this; - }, - - _mouseStart: function(event) { - var self = this; - - this.opos = [event.pageX, event.pageY]; - - if (this.options.disabled) - return; - - var options = this.options; - - this.selectees = $(options.filter, this.element[0]); - - this._trigger("start", event); - - $(options.appendTo).append(this.helper); - // position helper (lasso) - this.helper.css({ - "z-index": 100, - "position": "absolute", - "left": event.clientX, - "top": event.clientY, - "width": 0, - "height": 0 - }); - - if (options.autoRefresh) { - this.refresh(); - } - - this.selectees.filter('.ui-selected').each(function() { - var selectee = $.data(this, "selectable-item"); - selectee.startselected = true; - if (!event.metaKey) { - selectee.$element.removeClass('ui-selected'); - selectee.selected = false; - selectee.$element.addClass('ui-unselecting'); - selectee.unselecting = true; - // selectable UNSELECTING callback - self._trigger("unselecting", event, { - unselecting: selectee.element - }); - } - }); - - $(event.target).parents().andSelf().each(function() { - var selectee = $.data(this, "selectable-item"); - if (selectee) { - var doSelect = !event.metaKey || !selectee.$element.hasClass('ui-selected'); - selectee.$element - .removeClass(doSelect ? "ui-unselecting" : "ui-selected") - .addClass(doSelect ? "ui-selecting" : "ui-unselecting"); - selectee.unselecting = !doSelect; - selectee.selecting = doSelect; - selectee.selected = doSelect; - // selectable (UN)SELECTING callback - if (doSelect) { - self._trigger("selecting", event, { - selecting: selectee.element - }); - } else { - self._trigger("unselecting", event, { - unselecting: selectee.element - }); - } - return false; - } - }); - - }, - - _mouseDrag: function(event) { - var self = this; - this.dragged = true; - - if (this.options.disabled) - return; - - var options = this.options; - - var x1 = this.opos[0], y1 = this.opos[1], x2 = event.pageX, y2 = event.pageY; - if (x1 > x2) { var tmp = x2; x2 = x1; x1 = tmp; } - if (y1 > y2) { var tmp = y2; y2 = y1; y1 = tmp; } - this.helper.css({left: x1, top: y1, width: x2-x1, height: y2-y1}); - - this.selectees.each(function() { - var selectee = $.data(this, "selectable-item"); - //prevent helper from being selected if appendTo: selectable - if (!selectee || selectee.element == self.element[0]) - return; - var hit = false; - if (options.tolerance == 'touch') { - hit = ( !(selectee.left > x2 || selectee.right < x1 || selectee.top > y2 || selectee.bottom < y1) ); - } else if (options.tolerance == 'fit') { - hit = (selectee.left > x1 && selectee.right < x2 && selectee.top > y1 && selectee.bottom < y2); - } - - if (hit) { - // SELECT - if (selectee.selected) { - selectee.$element.removeClass('ui-selected'); - selectee.selected = false; - } - if (selectee.unselecting) { - selectee.$element.removeClass('ui-unselecting'); - selectee.unselecting = false; - } - if (!selectee.selecting) { - selectee.$element.addClass('ui-selecting'); - selectee.selecting = true; - // selectable SELECTING callback - self._trigger("selecting", event, { - selecting: selectee.element - }); - } - } else { - // UNSELECT - if (selectee.selecting) { - if (event.metaKey && selectee.startselected) { - selectee.$element.removeClass('ui-selecting'); - selectee.selecting = false; - selectee.$element.addClass('ui-selected'); - selectee.selected = true; - } else { - selectee.$element.removeClass('ui-selecting'); - selectee.selecting = false; - if (selectee.startselected) { - selectee.$element.addClass('ui-unselecting'); - selectee.unselecting = true; - } - // selectable UNSELECTING callback - self._trigger("unselecting", event, { - unselecting: selectee.element - }); - } - } - if (selectee.selected) { - if (!event.metaKey && !selectee.startselected) { - selectee.$element.removeClass('ui-selected'); - selectee.selected = false; - - selectee.$element.addClass('ui-unselecting'); - selectee.unselecting = true; - // selectable UNSELECTING callback - self._trigger("unselecting", event, { - unselecting: selectee.element - }); - } - } - } - }); - - return false; - }, - - _mouseStop: function(event) { - var self = this; - - this.dragged = false; - - var options = this.options; - - $('.ui-unselecting', this.element[0]).each(function() { - var selectee = $.data(this, "selectable-item"); - selectee.$element.removeClass('ui-unselecting'); - selectee.unselecting = false; - selectee.startselected = false; - self._trigger("unselected", event, { - unselected: selectee.element - }); - }); - $('.ui-selecting', this.element[0]).each(function() { - var selectee = $.data(this, "selectable-item"); - selectee.$element.removeClass('ui-selecting').addClass('ui-selected'); - selectee.selecting = false; - selectee.selected = true; - selectee.startselected = true; - self._trigger("selected", event, { - selected: selectee.element - }); - }); - this._trigger("stop", event); - - this.helper.remove(); - - return false; - } - -}); - -$.extend($.ui.selectable, { - version: "1.8.2" -}); - -})(jQuery); -(function(e){e.widget("ui.selectable",e.ui.mouse,{options:{appendTo:"body",autoRefresh:true,distance:0,filter:"*",tolerance:"touch"},_create:function(){var c=this;this.element.addClass("ui-selectable");this.dragged=false;var f;this.refresh=function(){f=e(c.options.filter,c.element[0]);f.each(function(){var d=e(this),b=d.offset();e.data(this,"selectable-item",{element:this,$element:d,left:b.left,top:b.top,right:b.left+d.outerWidth(),bottom:b.top+d.outerHeight(),startselected:false,selected:d.hasClass("ui-selected"), -selecting:d.hasClass("ui-selecting"),unselecting:d.hasClass("ui-unselecting")})})};this.refresh();this.selectees=f.addClass("ui-selectee");this._mouseInit();this.helper=e("
")},destroy:function(){this.selectees.removeClass("ui-selectee").removeData("selectable-item");this.element.removeClass("ui-selectable ui-selectable-disabled").removeData("selectable").unbind(".selectable");this._mouseDestroy();return this},_mouseStart:function(c){var f=this;this.opos=[c.pageX, -c.pageY];if(!this.options.disabled){var d=this.options;this.selectees=e(d.filter,this.element[0]);this._trigger("start",c);e(d.appendTo).append(this.helper);this.helper.css({"z-index":100,position:"absolute",left:c.clientX,top:c.clientY,width:0,height:0});d.autoRefresh&&this.refresh();this.selectees.filter(".ui-selected").each(function(){var b=e.data(this,"selectable-item");b.startselected=true;if(!c.metaKey){b.$element.removeClass("ui-selected");b.selected=false;b.$element.addClass("ui-unselecting"); -b.unselecting=true;f._trigger("unselecting",c,{unselecting:b.element})}});e(c.target).parents().andSelf().each(function(){var b=e.data(this,"selectable-item");if(b){var g=!c.metaKey||!b.$element.hasClass("ui-selected");b.$element.removeClass(g?"ui-unselecting":"ui-selected").addClass(g?"ui-selecting":"ui-unselecting");b.unselecting=!g;b.selecting=g;(b.selected=g)?f._trigger("selecting",c,{selecting:b.element}):f._trigger("unselecting",c,{unselecting:b.element});return false}})}},_mouseDrag:function(c){var f= -this;this.dragged=true;if(!this.options.disabled){var d=this.options,b=this.opos[0],g=this.opos[1],h=c.pageX,i=c.pageY;if(b>h){var j=h;h=b;b=j}if(g>i){j=i;i=g;g=j}this.helper.css({left:b,top:g,width:h-b,height:i-g});this.selectees.each(function(){var a=e.data(this,"selectable-item");if(!(!a||a.element==f.element[0])){var k=false;if(d.tolerance=="touch")k=!(a.left>h||a.righti||a.bottomb&&a.rightg&&a.bottom *",opacity:false,placeholder:false,revert:false,scroll:true,scrollSensitivity:20,scrollSpeed:20,scope:"default",tolerance:"intersect",zIndex:1E3},_create:function(){this.containerCache={};this.element.addClass("ui-sortable"); -this.refresh();this.floating=this.items.length?/left|right/.test(this.items[0].item.css("float")):false;this.offset=this.element.offset();this._mouseInit()},destroy:function(){this.element.removeClass("ui-sortable ui-sortable-disabled").removeData("sortable").unbind(".sortable");this._mouseDestroy();for(var a=this.items.length-1;a>=0;a--)this.items[a].item.removeData("sortable-item");return this},_setOption:function(a,b){if(a==="disabled"){this.options[a]=b;this.widget()[b?"addClass":"removeClass"]("ui-sortable-disabled")}else d.Widget.prototype._setOption.apply(this, -arguments)},_mouseCapture:function(a,b){if(this.reverting)return false;if(this.options.disabled||this.options.type=="static")return false;this._refreshItems(a);var c=null,e=this;d(a.target).parents().each(function(){if(d.data(this,"sortable-item")==e){c=d(this);return false}});if(d.data(a.target,"sortable-item")==e)c=d(a.target);if(!c)return false;if(this.options.handle&&!b){var f=false;d(this.options.handle,c).find("*").andSelf().each(function(){if(this==a.target)f=true});if(!f)return false}this.currentItem= -c;this._removeCurrentsFromItems();return true},_mouseStart:function(a,b,c){b=this.options;var e=this;this.currentContainer=this;this.refreshPositions();this.helper=this._createHelper(a);this._cacheHelperProportions();this._cacheMargins();this.scrollParent=this.helper.scrollParent();this.offset=this.currentItem.offset();this.offset={top:this.offset.top-this.margins.top,left:this.offset.left-this.margins.left};this.helper.css("position","absolute");this.cssPosition=this.helper.css("position");d.extend(this.offset, -{click:{left:a.pageX-this.offset.left,top:a.pageY-this.offset.top},parent:this._getParentOffset(),relative:this._getRelativeOffset()});this.originalPosition=this._generatePosition(a);this.originalPageX=a.pageX;this.originalPageY=a.pageY;b.cursorAt&&this._adjustOffsetFromHelper(b.cursorAt);this.domPosition={prev:this.currentItem.prev()[0],parent:this.currentItem.parent()[0]};this.helper[0]!=this.currentItem[0]&&this.currentItem.hide();this._createPlaceholder();b.containment&&this._setContainment(); -if(b.cursor){if(d("body").css("cursor"))this._storedCursor=d("body").css("cursor");d("body").css("cursor",b.cursor)}if(b.opacity){if(this.helper.css("opacity"))this._storedOpacity=this.helper.css("opacity");this.helper.css("opacity",b.opacity)}if(b.zIndex){if(this.helper.css("zIndex"))this._storedZIndex=this.helper.css("zIndex");this.helper.css("zIndex",b.zIndex)}if(this.scrollParent[0]!=document&&this.scrollParent[0].tagName!="HTML")this.overflowOffset=this.scrollParent.offset();this._trigger("start", -a,this._uiHash());this._preserveHelperProportions||this._cacheHelperProportions();if(!c)for(c=this.containers.length-1;c>=0;c--)this.containers[c]._trigger("activate",a,e._uiHash(this));if(d.ui.ddmanager)d.ui.ddmanager.current=this;d.ui.ddmanager&&!b.dropBehaviour&&d.ui.ddmanager.prepareOffsets(this,a);this.dragging=true;this.helper.addClass("ui-sortable-helper");this._mouseDrag(a);return true},_mouseDrag:function(a){this.position=this._generatePosition(a);this.positionAbs=this._convertPositionTo("absolute"); -if(!this.lastPositionAbs)this.lastPositionAbs=this.positionAbs;if(this.options.scroll){var b=this.options,c=false;if(this.scrollParent[0]!=document&&this.scrollParent[0].tagName!="HTML"){if(this.overflowOffset.top+this.scrollParent[0].offsetHeight-a.pageY=0;b--){c=this.items[b];var e=c.item[0],f=this._intersectsWithPointer(c);if(f)if(e!=this.currentItem[0]&&this.placeholder[f==1?"next":"prev"]()[0]!=e&&!d.ui.contains(this.placeholder[0],e)&&(this.options.type=="semi-dynamic"?!d.ui.contains(this.element[0],e):true)){this.direction=f==1?"down":"up";if(this.options.tolerance=="pointer"||this._intersectsWithSides(c))this._rearrange(a, -c);else break;this._trigger("change",a,this._uiHash());break}}this._contactContainers(a);d.ui.ddmanager&&d.ui.ddmanager.drag(this,a);this._trigger("sort",a,this._uiHash());this.lastPositionAbs=this.positionAbs;return false},_mouseStop:function(a,b){if(a){d.ui.ddmanager&&!this.options.dropBehaviour&&d.ui.ddmanager.drop(this,a);if(this.options.revert){var c=this;b=c.placeholder.offset();c.reverting=true;d(this.helper).animate({left:b.left-this.offset.parent.left-c.margins.left+(this.offsetParent[0]== -document.body?0:this.offsetParent[0].scrollLeft),top:b.top-this.offset.parent.top-c.margins.top+(this.offsetParent[0]==document.body?0:this.offsetParent[0].scrollTop)},parseInt(this.options.revert,10)||500,function(){c._clear(a)})}else this._clear(a,b);return false}},cancel:function(){var a=this;if(this.dragging){this._mouseUp();this.options.helper=="original"?this.currentItem.css(this._storedCSS).removeClass("ui-sortable-helper"):this.currentItem.show();for(var b=this.containers.length-1;b>=0;b--){this.containers[b]._trigger("deactivate", 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b=this._getItemsAsjQuery(a&&a.connected),c=[];a=a||{};b.each(function(){c.push(d(a.item||this).attr(a.attribute||"id")||"")});return c}, -_intersectsWith:function(a){var b=this.positionAbs.left,c=b+this.helperProportions.width,e=this.positionAbs.top,f=e+this.helperProportions.height,g=a.left,h=g+a.width,i=a.top,k=i+a.height,j=this.offset.click.top,l=this.offset.click.left;j=e+j>i&&e+jg&&b+la[this.floating?"width":"height"]?j:g0?"down":"up")},_getDragHorizontalDirection:function(){var a= -this.positionAbs.left-this.lastPositionAbs.left;return a!=0&&(a>0?"right":"left")},refresh:function(a){this._refreshItems(a);this.refreshPositions();return this},_connectWith:function(){var a=this.options;return a.connectWith.constructor==String?[a.connectWith]:a.connectWith},_getItemsAsjQuery:function(a){var b=[],c=[],e=this._connectWith();if(e&&a)for(a=e.length-1;a>=0;a--)for(var f=d(e[a]),g=f.length-1;g>=0;g--){var h=d.data(f[g],"sortable");if(h&&h!=this&&!h.options.disabled)c.push([d.isFunction(h.options.items)? -h.options.items.call(h.element):d(h.options.items,h.element).not(".ui-sortable-helper").not(".ui-sortable-placeholder"),h])}c.push([d.isFunction(this.options.items)?this.options.items.call(this.element,null,{options:this.options,item:this.currentItem}):d(this.options.items,this.element).not(".ui-sortable-helper").not(".ui-sortable-placeholder"),this]);for(a=c.length-1;a>=0;a--)c[a][0].each(function(){b.push(this)});return d(b)},_removeCurrentsFromItems:function(){for(var a=this.currentItem.find(":data(sortable-item)"), -b=0;b=0;f--)for(var g=d(e[f]),h=g.length-1;h>=0;h--){var i=d.data(g[h],"sortable");if(i&&i!=this&&!i.options.disabled){c.push([d.isFunction(i.options.items)? -i.options.items.call(i.element[0],a,{item:this.currentItem}):d(i.options.items,i.element),i]);this.containers.push(i)}}for(f=c.length-1;f>=0;f--){a=c[f][1];e=c[f][0];h=0;for(g=e.length;h=0;b--){var c=this.items[b],e=this.options.toleranceElement?d(this.options.toleranceElement, -c.item):c.item;if(!a){c.width=e.outerWidth();c.height=e.outerHeight()}e=e.offset();c.left=e.left;c.top=e.top}if(this.options.custom&&this.options.custom.refreshContainers)this.options.custom.refreshContainers.call(this);else for(b=this.containers.length-1;b>=0;b--){e=this.containers[b].element.offset();this.containers[b].containerCache.left=e.left;this.containers[b].containerCache.top=e.top;this.containers[b].containerCache.width=this.containers[b].element.outerWidth();this.containers[b].containerCache.height= -this.containers[b].element.outerHeight()}return this},_createPlaceholder:function(a){var b=a||this,c=b.options;if(!c.placeholder||c.placeholder.constructor==String){var e=c.placeholder;c.placeholder={element:function(){var f=d(document.createElement(b.currentItem[0].nodeName)).addClass(e||b.currentItem[0].className+" 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h=this.items[g][this.containers[c].floating?"left":"top"];if(Math.abs(h-f)this.containment[2])f=this.containment[2]+this.offset.click.left;if(a.pageY-this.offset.click.top>this.containment[3])g=this.containment[3]+this.offset.click.top}if(b.grid){g=this.originalPageY+Math.round((g-this.originalPageY)/b.grid[1])*b.grid[1];g=this.containment?!(g-this.offset.click.topthis.containment[3])?g:!(g-this.offset.click.topthis.containment[2])?f:!(f-this.offset.click.left=0;e--)if(d.ui.contains(this.containers[e].element[0],this.currentItem[0])&&!b){c.push(function(f){return function(g){f._trigger("receive",g,this._uiHash(this))}}.call(this,this.containers[e]));c.push(function(f){return function(g){f._trigger("update", -g,this._uiHash(this))}}.call(this,this.containers[e]))}}for(e=this.containers.length-1;e>=0;e--){b||c.push(function(f){return function(g){f._trigger("deactivate",g,this._uiHash(this))}}.call(this,this.containers[e]));if(this.containers[e].containerCache.over){c.push(function(f){return function(g){f._trigger("out",g,this._uiHash(this))}}.call(this,this.containers[e]));this.containers[e].containerCache.over=0}}this._storedCursor&&d("body").css("cursor",this._storedCursor);this._storedOpacity&&this.helper.css("opacity", -this._storedOpacity);if(this._storedZIndex)this.helper.css("zIndex",this._storedZIndex=="auto"?"":this._storedZIndex);this.dragging=false;if(this.cancelHelperRemoval){if(!b){this._trigger("beforeStop",a,this._uiHash());for(e=0;e li > :first-child,> :not(li):even",icons:{header:"ui-icon-triangle-1-e",headerSelected:"ui-icon-triangle-1-s"},navigation:false,navigationFilter:function(){return this.href.toLowerCase()==location.href.toLowerCase()}},_create:function(){var a=this.options,b=this;this.running=0;this.element.addClass("ui-accordion ui-widget ui-helper-reset"); 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f=d.closest(".ui-accordion-header");this.active=f.length?f:d.closest(".ui-accordion-content").prev()}}this.active=this._findActive(this.active||a.active).toggleClass("ui-state-default").toggleClass("ui-state-active").toggleClass("ui-corner-all").toggleClass("ui-corner-top");this.active.next().addClass("ui-accordion-content-active");this._createIcons();this.resize();this.element.attr("role","tablist");this.headers.attr("role", -"tab").bind("keydown",function(g){return b._keydown(g)}).next().attr("role","tabpanel");this.headers.not(this.active||"").attr("aria-expanded","false").attr("tabIndex","-1").next().hide();this.active.length?this.active.attr("aria-expanded","true").attr("tabIndex","0"):this.headers.eq(0).attr("tabIndex","0");c.browser.safari||this.headers.find("a").attr("tabIndex","-1");a.event&&this.headers.bind(a.event+".accordion",function(g){b._clickHandler.call(b,g,this);g.preventDefault()})},_createIcons:function(){var a= -this.options;if(a.icons){c("").addClass("ui-icon 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this},_setOption:function(a,b){c.Widget.prototype._setOption.apply(this,arguments);a=="active"&&this.activate(b);if(a=="icons"){this._destroyIcons();b&&this._createIcons()}},_keydown:function(a){var b=c.ui.keyCode;if(!(this.options.disabled||a.altKey||a.ctrlKey)){var d=this.headers.length,f=this.headers.index(a.target),g=false;switch(a.keyCode){case b.RIGHT:case b.DOWN:g=this.headers[(f+1)%d];break;case b.LEFT:case b.UP:g=this.headers[(f-1+d)%d];break;case b.SPACE:case b.ENTER:this._clickHandler({target:a.target}, -a.target);a.preventDefault()}if(g){c(a.target).attr("tabIndex","-1");c(g).attr("tabIndex","0");g.focus();return false}return true}},resize:function(){var a=this.options,b;if(a.fillSpace){if(c.browser.msie){var d=this.element.parent().css("overflow");this.element.parent().css("overflow","hidden")}b=this.element.parent().height();c.browser.msie&&this.element.parent().css("overflow",d);this.headers.each(function(){b-=c(this).outerHeight(true)});this.headers.next().each(function(){c(this).height(Math.max(0, -b-c(this).innerHeight()+c(this).height()))}).css("overflow","auto")}else if(a.autoHeight){b=0;this.headers.next().each(function(){b=Math.max(b,c(this).height())}).height(b)}return this},activate:function(a){this.options.active=a;a=this._findActive(a)[0];this._clickHandler({target:a},a);return this},_findActive:function(a){return a?typeof a=="number"?this.headers.filter(":eq("+a+")"):this.headers.not(this.headers.not(a)):a===false?c([]):this.headers.filter(":eq(0)")},_clickHandler:function(a,b){var d= -this.options;if(!d.disabled)if(a.target){a=c(a.currentTarget||b);b=a[0]==this.active[0];d.active=d.collapsible&&b?false:c(".ui-accordion-header",this.element).index(a);if(!(this.running||!d.collapsible&&b)){this.active.removeClass("ui-state-active ui-corner-top").addClass("ui-state-default ui-corner-all").find(".ui-icon").removeClass(d.icons.headerSelected).addClass(d.icons.header);if(!b){a.removeClass("ui-state-default ui-corner-all").addClass("ui-state-active ui-corner-top").find(".ui-icon").removeClass(d.icons.header).addClass(d.icons.headerSelected); -a.next().addClass("ui-accordion-content-active")}e=a.next();f=this.active.next();g={options:d,newHeader:b&&d.collapsible?c([]):a,oldHeader:this.active,newContent:b&&d.collapsible?c([]):e,oldContent:f};d=this.headers.index(this.active[0])>this.headers.index(a[0]);this.active=b?c([]):a;this._toggle(e,f,g,b,d)}}else if(d.collapsible){this.active.removeClass("ui-state-active ui-corner-top").addClass("ui-state-default ui-corner-all").find(".ui-icon").removeClass(d.icons.headerSelected).addClass(d.icons.header); -this.active.next().addClass("ui-accordion-content-active");var f=this.active.next(),g={options:d,newHeader:c([]),oldHeader:d.active,newContent:c([]),oldContent:f},e=this.active=c([]);this._toggle(e,f,g)}},_toggle:function(a,b,d,f,g){var e=this.options,k=this;this.toShow=a;this.toHide=b;this.data=d;var i=function(){if(k)return k._completed.apply(k,arguments)};this._trigger("changestart",null,this.data);this.running=b.size()===0?a.size():b.size();if(e.animated){d={};d=e.collapsible&&f?{toShow:c([]), -toHide:b,complete:i,down:g,autoHeight:e.autoHeight||e.fillSpace}:{toShow:a,toHide:b,complete:i,down:g,autoHeight:e.autoHeight||e.fillSpace};if(!e.proxied)e.proxied=e.animated;if(!e.proxiedDuration)e.proxiedDuration=e.duration;e.animated=c.isFunction(e.proxied)?e.proxied(d):e.proxied;e.duration=c.isFunction(e.proxiedDuration)?e.proxiedDuration(d):e.proxiedDuration;f=c.ui.accordion.animations;var h=e.duration,j=e.animated;if(j&&!f[j]&&!c.easing[j])j="slide";f[j]||(f[j]=function(l){this.slide(l,{easing:j, -duration:h||700})});f[j](d)}else{if(e.collapsible&&f)a.toggle();else{b.hide();a.show()}i(true)}b.prev().attr("aria-expanded","false").attr("tabIndex","-1").blur();a.prev().attr("aria-expanded","true").attr("tabIndex","0").focus()},_completed:function(a){var b=this.options;this.running=a?0:--this.running;if(!this.running){b.clearStyle&&this.toShow.add(this.toHide).css({height:"",overflow:""});this.toHide.removeClass("ui-accordion-content-active");this._trigger("change",null,this.data)}}});c.extend(c.ui.accordion, -{version:"1.8.2",animations:{slide:function(a,b){a=c.extend({easing:"swing",duration:300},a,b);if(a.toHide.size())if(a.toShow.size()){var d=a.toShow.css("overflow"),f=0,g={},e={},k;b=a.toShow;k=b[0].style.width;b.width(parseInt(b.parent().width(),10)-parseInt(b.css("paddingLeft"),10)-parseInt(b.css("paddingRight"),10)-(parseInt(b.css("borderLeftWidth"),10)||0)-(parseInt(b.css("borderRightWidth"),10)||0));c.each(["height","paddingTop","paddingBottom"],function(i,h){e[h]="hide";i=(""+c.css(a.toShow[0], -h)).match(/^([\d+-.]+)(.*)$/);g[h]={value:i[1],unit:i[2]||"px"}});a.toShow.css({height:0,overflow:"hidden"}).show();a.toHide.filter(":hidden").each(a.complete).end().filter(":visible").animate(e,{step:function(i,h){if(h.prop=="height")f=h.end-h.start===0?0:(h.now-h.start)/(h.end-h.start);a.toShow[0].style[h.prop]=f*g[h.prop].value+g[h.prop].unit},duration:a.duration,easing:a.easing,complete:function(){a.autoHeight||a.toShow.css("height","");a.toShow.css("width",k);a.toShow.css({overflow:d});a.complete()}})}else a.toHide.animate({height:"hide"}, -a);else a.toShow.animate({height:"show"},a)},bounceslide:function(a){this.slide(a,{easing:a.down?"easeOutBounce":"swing",duration:a.down?1E3:200})}}})})(jQuery); -;/* - * jQuery UI Autocomplete 1.8.2 - * - * Copyright (c) 2010 AUTHORS.txt (http://jqueryui.com/about) - * Dual licensed under the MIT (MIT-LICENSE.txt) - * and GPL (GPL-LICENSE.txt) licenses. - * - * http://docs.jquery.com/UI/Autocomplete - * - * Depends: - * jquery.ui.core.js - * jquery.ui.widget.js - * jquery.ui.position.js - */ -(function(e){e.widget("ui.autocomplete",{options:{minLength:1,delay:300},_create:function(){var a=this,c=this.element[0].ownerDocument;this.element.addClass("ui-autocomplete-input").attr("autocomplete","off").attr({role:"textbox","aria-autocomplete":"list","aria-haspopup":"true"}).bind("keydown.autocomplete",function(d){var b=e.ui.keyCode;switch(d.keyCode){case b.PAGE_UP:a._move("previousPage",d);break;case b.PAGE_DOWN:a._move("nextPage",d);break;case b.UP:a._move("previous",d);d.preventDefault(); -break;case b.DOWN:a._move("next",d);d.preventDefault();break;case b.ENTER:case b.NUMPAD_ENTER:a.menu.active&&d.preventDefault();case b.TAB:if(!a.menu.active)return;a.menu.select(d);break;case b.ESCAPE:a.element.val(a.term);a.close(d);break;case b.LEFT:case b.RIGHT:case b.SHIFT:case b.CONTROL:case b.ALT:case b.COMMAND:case b.COMMAND_RIGHT:case b.INSERT:case b.CAPS_LOCK:case b.END:case b.HOME:break;default:clearTimeout(a.searching);a.searching=setTimeout(function(){a.search(null,d)},a.options.delay); -break}}).bind("focus.autocomplete",function(){a.selectedItem=null;a.previous=a.element.val()}).bind("blur.autocomplete",function(d){clearTimeout(a.searching);a.closing=setTimeout(function(){a.close(d);a._change(d)},150)});this._initSource();this.response=function(){return a._response.apply(a,arguments)};this.menu=e("
    ").addClass("ui-autocomplete").appendTo("body",c).mousedown(function(){setTimeout(function(){clearTimeout(a.closing)},13)}).menu({focus:function(d,b){b=b.item.data("item.autocomplete"); -false!==a._trigger("focus",null,{item:b})&&/^key/.test(d.originalEvent.type)&&a.element.val(b.value)},selected:function(d,b){b=b.item.data("item.autocomplete");false!==a._trigger("select",d,{item:b})&&a.element.val(b.value);a.close(d);d=a.previous;if(a.element[0]!==c.activeElement){a.element.focus();a.previous=d}a.selectedItem=b},blur:function(){a.menu.element.is(":visible")&&a.element.val(a.term)}}).zIndex(this.element.zIndex()+1).css({top:0,left:0}).hide().data("menu");e.fn.bgiframe&&this.menu.element.bgiframe()}, -destroy:function(){this.element.removeClass("ui-autocomplete-input").removeAttr("autocomplete").removeAttr("role").removeAttr("aria-autocomplete").removeAttr("aria-haspopup");this.menu.element.remove();e.Widget.prototype.destroy.call(this)},_setOption:function(a){e.Widget.prototype._setOption.apply(this,arguments);a==="source"&&this._initSource()},_initSource:function(){var a,c;if(e.isArray(this.options.source)){a=this.options.source;this.source=function(d,b){b(e.ui.autocomplete.filter(a,d.term))}}else if(typeof this.options.source=== -"string"){c=this.options.source;this.source=function(d,b){e.getJSON(c,d,b)}}else this.source=this.options.source},search:function(a,c){a=a!=null?a:this.element.val();if(a.length").data("item.autocomplete", -c).append(""+c.label+"").appendTo(a)},_move:function(a,c){if(this.menu.element.is(":visible"))if(this.menu.first()&&/^previous/.test(a)||this.menu.last()&&/^next/.test(a)){this.element.val(this.term);this.menu.deactivate()}else this.menu[a](c);else this.search(null,c)},widget:function(){return this.menu.element}});e.extend(e.ui.autocomplete,{escapeRegex:function(a){return a.replace(/([\^\$\(\)\[\]\{\}\*\.\+\?\|\\])/gi,"\\$1")},filter:function(a,c){var d=new RegExp(e.ui.autocomplete.escapeRegex(c), -"i");return e.grep(a,function(b){return d.test(b.label||b.value||b)})}})})(jQuery); -(function(e){e.widget("ui.menu",{_create:function(){var a=this;this.element.addClass("ui-menu ui-widget ui-widget-content ui-corner-all").attr({role:"listbox","aria-activedescendant":"ui-active-menuitem"}).click(function(c){if(e(c.target).closest(".ui-menu-item a").length){c.preventDefault();a.select(c)}});this.refresh()},refresh:function(){var a=this;this.element.children("li:not(.ui-menu-item):has(a)").addClass("ui-menu-item").attr("role","menuitem").children("a").addClass("ui-corner-all").attr("tabindex", --1).mouseenter(function(c){a.activate(c,e(this).parent())}).mouseleave(function(){a.deactivate()})},activate:function(a,c){this.deactivate();if(this.hasScroll()){var d=c.offset().top-this.element.offset().top,b=this.element.attr("scrollTop"),f=this.element.height();if(d<0)this.element.attr("scrollTop",b+d);else d>f&&this.element.attr("scrollTop",b+d-f+c.height())}this.active=c.eq(0).children("a").addClass("ui-state-hover").attr("id","ui-active-menuitem").end();this._trigger("focus",a,{item:c})},deactivate:function(){if(this.active){this.active.children("a").removeClass("ui-state-hover").removeAttr("id"); -this._trigger("blur");this.active=null}},next:function(a){this.move("next",".ui-menu-item:first",a)},previous:function(a){this.move("prev",".ui-menu-item:last",a)},first:function(){return this.active&&!this.active.prev().length},last:function(){return this.active&&!this.active.next().length},move:function(a,c,d){if(this.active){a=this.active[a+"All"](".ui-menu-item").eq(0);a.length?this.activate(d,a):this.activate(d,this.element.children(c))}else this.activate(d,this.element.children(c))},nextPage:function(a){if(this.hasScroll())if(!this.active|| -this.last())this.activate(a,this.element.children(":first"));else{var c=this.active.offset().top,d=this.element.height(),b=this.element.children("li").filter(function(){var f=e(this).offset().top-c-d+e(this).height();return f<10&&f>-10});b.length||(b=this.element.children(":last"));this.activate(a,b)}else this.activate(a,this.element.children(!this.active||this.last()?":first":":last"))},previousPage:function(a){if(this.hasScroll())if(!this.active||this.first())this.activate(a,this.element.children(":last")); -else{var c=this.active.offset().top,d=this.element.height();result=this.element.children("li").filter(function(){var b=e(this).offset().top-c+d-e(this).height();return b<10&&b>-10});result.length||(result=this.element.children(":first"));this.activate(a,result)}else this.activate(a,this.element.children(!this.active||this.first()?":last":":first"))},hasScroll:function(){return this.element.height()    ").addClass("ui-button-text").html(this.options.label).appendTo(b.empty()).text(),d=this.options.icons,e=d.primary&&d.secondary;if(d.primary||d.secondary){b.addClass("ui-button-text-icon"+(e?"s":""));d.primary&&b.prepend("");d.secondary&&b.append("");if(!this.options.text){b.addClass(e?"ui-button-icons-only":"ui-button-icon-only").removeClass("ui-button-text-icons ui-button-text-icon"); -this.hasTitle||b.attr("title",c)}}else b.addClass("ui-button-text-only")}}});a.widget("ui.buttonset",{_create:function(){this.element.addClass("ui-buttonset");this._init()},_init:function(){this.refresh()},_setOption:function(b,c){b==="disabled"&&this.buttons.button("option",b,c);a.Widget.prototype._setOption.apply(this,arguments)},refresh:function(){this.buttons=this.element.find(":button, :submit, :reset, :checkbox, :radio, a, :data(button)").filter(":ui-button").button("refresh").end().not(":ui-button").button().end().map(function(){return a(this).button("widget")[0]}).removeClass("ui-corner-all ui-corner-left ui-corner-right").filter(":first").addClass("ui-corner-left").end().filter(":last").addClass("ui-corner-right").end().end()}, -destroy:function(){this.element.removeClass("ui-buttonset");this.buttons.map(function(){return a(this).button("widget")[0]}).removeClass("ui-corner-left ui-corner-right").end().button("destroy");a.Widget.prototype.destroy.call(this)}})})(jQuery); -;/* - * jQuery UI Dialog 1.8.2 - * - * Copyright (c) 2010 AUTHORS.txt (http://jqueryui.com/about) - * Dual licensed under the MIT (MIT-LICENSE.txt) - * and GPL (GPL-LICENSE.txt) licenses. - * - * http://docs.jquery.com/UI/Dialog - * - * Depends: - * jquery.ui.core.js - * jquery.ui.widget.js - * jquery.ui.button.js - * jquery.ui.draggable.js - * jquery.ui.mouse.js - * jquery.ui.position.js - * jquery.ui.resizable.js - */ -(function(c){c.widget("ui.dialog",{options:{autoOpen:true,buttons:{},closeOnEscape:true,closeText:"close",dialogClass:"",draggable:true,hide:null,height:"auto",maxHeight:false,maxWidth:false,minHeight:150,minWidth:150,modal:false,position:"center",resizable:true,show:null,stack:true,title:"",width:300,zIndex:1E3},_create:function(){this.originalTitle=this.element.attr("title");var a=this,b=a.options,d=b.title||a.originalTitle||" ",e=c.ui.dialog.getTitleId(a.element),g=(a.uiDialog=c("
    ")).appendTo(document.body).hide().addClass("ui-dialog ui-widget ui-widget-content ui-corner-all "+ -b.dialogClass).css({zIndex:b.zIndex}).attr("tabIndex",-1).css("outline",0).keydown(function(i){if(b.closeOnEscape&&i.keyCode&&i.keyCode===c.ui.keyCode.ESCAPE){a.close(i);i.preventDefault()}}).attr({role:"dialog","aria-labelledby":e}).mousedown(function(i){a.moveToTop(false,i)});a.element.show().removeAttr("title").addClass("ui-dialog-content ui-widget-content").appendTo(g);var f=(a.uiDialogTitlebar=c("
    ")).addClass("ui-dialog-titlebar ui-widget-header ui-corner-all ui-helper-clearfix").prependTo(g), -h=c('').addClass("ui-dialog-titlebar-close ui-corner-all").attr("role","button").hover(function(){h.addClass("ui-state-hover")},function(){h.removeClass("ui-state-hover")}).focus(function(){h.addClass("ui-state-focus")}).blur(function(){h.removeClass("ui-state-focus")}).click(function(i){a.close(i);return false}).appendTo(f);(a.uiDialogTitlebarCloseText=c("")).addClass("ui-icon ui-icon-closethick").text(b.closeText).appendTo(h);c("").addClass("ui-dialog-title").attr("id", -e).html(d).prependTo(f);if(c.isFunction(b.beforeclose)&&!c.isFunction(b.beforeClose))b.beforeClose=b.beforeclose;f.find("*").add(f).disableSelection();b.draggable&&c.fn.draggable&&a._makeDraggable();b.resizable&&c.fn.resizable&&a._makeResizable();a._createButtons(b.buttons);a._isOpen=false;c.fn.bgiframe&&g.bgiframe()},_init:function(){this.options.autoOpen&&this.open()},destroy:function(){var a=this;a.overlay&&a.overlay.destroy();a.uiDialog.hide();a.element.unbind(".dialog").removeData("dialog").removeClass("ui-dialog-content ui-widget-content").hide().appendTo("body"); -a.uiDialog.remove();a.originalTitle&&a.element.attr("title",a.originalTitle);return a},widget:function(){return this.uiDialog},close:function(a){var b=this,d;if(false!==b._trigger("beforeClose",a)){b.overlay&&b.overlay.destroy();b.uiDialog.unbind("keypress.ui-dialog");b._isOpen=false;if(b.options.hide)b.uiDialog.hide(b.options.hide,function(){b._trigger("close",a)});else{b.uiDialog.hide();b._trigger("close",a)}c.ui.dialog.overlay.resize();if(b.options.modal){d=0;c(".ui-dialog").each(function(){if(this!== -b.uiDialog[0])d=Math.max(d,c(this).css("z-index"))});c.ui.dialog.maxZ=d}return b}},isOpen:function(){return this._isOpen},moveToTop:function(a,b){var d=this,e=d.options;if(e.modal&&!a||!e.stack&&!e.modal)return d._trigger("focus",b);if(e.zIndex>c.ui.dialog.maxZ)c.ui.dialog.maxZ=e.zIndex;if(d.overlay){c.ui.dialog.maxZ+=1;d.overlay.$el.css("z-index",c.ui.dialog.overlay.maxZ=c.ui.dialog.maxZ)}a={scrollTop:d.element.attr("scrollTop"),scrollLeft:d.element.attr("scrollLeft")};c.ui.dialog.maxZ+=1;d.uiDialog.css("z-index", -c.ui.dialog.maxZ);d.element.attr(a);d._trigger("focus",b);return d},open:function(){if(!this._isOpen){var a=this,b=a.options,d=a.uiDialog;a.overlay=b.modal?new c.ui.dialog.overlay(a):null;d.next().length&&d.appendTo("body");a._size();a._position(b.position);d.show(b.show);a.moveToTop(true);b.modal&&d.bind("keypress.ui-dialog",function(e){if(e.keyCode===c.ui.keyCode.TAB){var g=c(":tabbable",this),f=g.filter(":first");g=g.filter(":last");if(e.target===g[0]&&!e.shiftKey){f.focus(1);return false}else if(e.target=== -f[0]&&e.shiftKey){g.focus(1);return false}}});c([]).add(d.find(".ui-dialog-content :tabbable:first")).add(d.find(".ui-dialog-buttonpane :tabbable:first")).add(d).filter(":first").focus();a._trigger("open");a._isOpen=true;return a}},_createButtons:function(a){var b=this,d=false,e=c("
    ").addClass("ui-dialog-buttonpane ui-widget-content ui-helper-clearfix");b.uiDialog.find(".ui-dialog-buttonpane").remove();typeof a==="object"&&a!==null&&c.each(a,function(){return!(d=true)});if(d){c.each(a, -function(g,f){g=c('').text(g).click(function(){f.apply(b.element[0],arguments)}).appendTo(e);c.fn.button&&g.button()});e.appendTo(b.uiDialog)}},_makeDraggable:function(){function a(f){return{position:f.position,offset:f.offset}}var b=this,d=b.options,e=c(document),g;b.uiDialog.draggable({cancel:".ui-dialog-content, .ui-dialog-titlebar-close",handle:".ui-dialog-titlebar",containment:"document",start:function(f,h){g=d.height==="auto"?"auto":c(this).height();c(this).height(c(this).height()).addClass("ui-dialog-dragging"); -b._trigger("dragStart",f,a(h))},drag:function(f,h){b._trigger("drag",f,a(h))},stop:function(f,h){d.position=[h.position.left-e.scrollLeft(),h.position.top-e.scrollTop()];c(this).removeClass("ui-dialog-dragging").height(g);b._trigger("dragStop",f,a(h));c.ui.dialog.overlay.resize()}})},_makeResizable:function(a){function b(f){return{originalPosition:f.originalPosition,originalSize:f.originalSize,position:f.position,size:f.size}}a=a===undefined?this.options.resizable:a;var d=this,e=d.options,g=d.uiDialog.css("position"); -a=typeof a==="string"?a:"n,e,s,w,se,sw,ne,nw";d.uiDialog.resizable({cancel:".ui-dialog-content",containment:"document",alsoResize:d.element,maxWidth:e.maxWidth,maxHeight:e.maxHeight,minWidth:e.minWidth,minHeight:d._minHeight(),handles:a,start:function(f,h){c(this).addClass("ui-dialog-resizing");d._trigger("resizeStart",f,b(h))},resize:function(f,h){d._trigger("resize",f,b(h))},stop:function(f,h){c(this).removeClass("ui-dialog-resizing");e.height=c(this).height();e.width=c(this).width();d._trigger("resizeStop", -f,b(h));c.ui.dialog.overlay.resize()}}).css("position",g).find(".ui-resizable-se").addClass("ui-icon ui-icon-grip-diagonal-se")},_minHeight:function(){var a=this.options;return a.height==="auto"?a.minHeight:Math.min(a.minHeight,a.height)},_position:function(a){var b=[],d=[0,0];a=a||c.ui.dialog.prototype.options.position;if(typeof a==="string"||typeof a==="object"&&"0"in a){b=a.split?a.split(" "):[a[0],a[1]];if(b.length===1)b[1]=b[0];c.each(["left","top"],function(e,g){if(+b[e]===b[e]){d[e]=b[e];b[e]= -g}})}else if(typeof a==="object"){if("left"in a){b[0]="left";d[0]=a.left}else if("right"in a){b[0]="right";d[0]=-a.right}if("top"in a){b[1]="top";d[1]=a.top}else if("bottom"in a){b[1]="bottom";d[1]=-a.bottom}}(a=this.uiDialog.is(":visible"))||this.uiDialog.show();this.uiDialog.css({top:0,left:0}).position({my:b.join(" "),at:b.join(" "),offset:d.join(" "),of:window,collision:"fit",using:function(e){var g=c(this).css(e).offset().top;g<0&&c(this).css("top",e.top-g)}});a||this.uiDialog.hide()},_setOption:function(a, -b){var d=this,e=d.uiDialog,g=e.is(":data(resizable)"),f=false;switch(a){case "beforeclose":a="beforeClose";break;case "buttons":d._createButtons(b);break;case "closeText":d.uiDialogTitlebarCloseText.text(""+b);break;case "dialogClass":e.removeClass(d.options.dialogClass).addClass("ui-dialog ui-widget ui-widget-content ui-corner-all "+b);break;case "disabled":b?e.addClass("ui-dialog-disabled"):e.removeClass("ui-dialog-disabled");break;case "draggable":b?d._makeDraggable():e.draggable("destroy");break; -case "height":f=true;break;case "maxHeight":g&&e.resizable("option","maxHeight",b);f=true;break;case "maxWidth":g&&e.resizable("option","maxWidth",b);f=true;break;case "minHeight":g&&e.resizable("option","minHeight",b);f=true;break;case "minWidth":g&&e.resizable("option","minWidth",b);f=true;break;case "position":d._position(b);break;case "resizable":g&&!b&&e.resizable("destroy");g&&typeof b==="string"&&e.resizable("option","handles",b);!g&&b!==false&&d._makeResizable(b);break;case "title":c(".ui-dialog-title", -d.uiDialogTitlebar).html(""+(b||" "));break;case "width":f=true;break}c.Widget.prototype._setOption.apply(d,arguments);f&&d._size()},_size:function(){var a=this.options,b;this.element.css({width:"auto",minHeight:0,height:0});b=this.uiDialog.css({height:"auto",width:a.width}).height();this.element.css(a.height==="auto"?{minHeight:Math.max(a.minHeight-b,0),height:"auto"}:{minHeight:0,height:Math.max(a.height-b,0)}).show();this.uiDialog.is(":data(resizable)")&&this.uiDialog.resizable("option","minHeight", -this._minHeight())}});c.extend(c.ui.dialog,{version:"1.8.2",uuid:0,maxZ:0,getTitleId:function(a){a=a.attr("id");if(!a){this.uuid+=1;a=this.uuid}return"ui-dialog-title-"+a},overlay:function(a){this.$el=c.ui.dialog.overlay.create(a)}});c.extend(c.ui.dialog.overlay,{instances:[],oldInstances:[],maxZ:0,events:c.map("focus,mousedown,mouseup,keydown,keypress,click".split(","),function(a){return a+".dialog-overlay"}).join(" "),create:function(a){if(this.instances.length===0){setTimeout(function(){c.ui.dialog.overlay.instances.length&& -c(document).bind(c.ui.dialog.overlay.events,function(d){return c(d.target).zIndex()>=c.ui.dialog.overlay.maxZ})},1);c(document).bind("keydown.dialog-overlay",function(d){if(a.options.closeOnEscape&&d.keyCode&&d.keyCode===c.ui.keyCode.ESCAPE){a.close(d);d.preventDefault()}});c(window).bind("resize.dialog-overlay",c.ui.dialog.overlay.resize)}var b=(this.oldInstances.pop()||c("
    ").addClass("ui-widget-overlay")).appendTo(document.body).css({width:this.width(),height:this.height()});c.fn.bgiframe&& -b.bgiframe();this.instances.push(b);return b},destroy:function(a){this.oldInstances.push(this.instances.splice(c.inArray(a,this.instances),1)[0]);this.instances.length===0&&c([document,window]).unbind(".dialog-overlay");a.remove();var b=0;c.each(this.instances,function(){b=Math.max(b,this.css("z-index"))});this.maxZ=b},height:function(){var a,b;if(c.browser.msie&&c.browser.version<7){a=Math.max(document.documentElement.scrollHeight,document.body.scrollHeight);b=Math.max(document.documentElement.offsetHeight, -document.body.offsetHeight);return a");if(!b.values)b.values=[this._valueMin(),this._valueMin()];if(b.values.length&&b.values.length!==2)b.values=[b.values[0],b.values[0]]}else this.range=d("
    ");this.range.appendTo(this.element).addClass("ui-slider-range");if(b.range==="min"||b.range==="max")this.range.addClass("ui-slider-range-"+b.range);this.range.addClass("ui-widget-header")}d(".ui-slider-handle",this.element).length===0&&d("").appendTo(this.element).addClass("ui-slider-handle"); -if(b.values&&b.values.length)for(;d(".ui-slider-handle",this.element).length").appendTo(this.element).addClass("ui-slider-handle");this.handles=d(".ui-slider-handle",this.element).addClass("ui-state-default ui-corner-all");this.handle=this.handles.eq(0);this.handles.add(this.range).filter("a").click(function(c){c.preventDefault()}).hover(function(){b.disabled||d(this).addClass("ui-state-hover")},function(){d(this).removeClass("ui-state-hover")}).focus(function(){if(b.disabled)d(this).blur(); -else{d(".ui-slider .ui-state-focus").removeClass("ui-state-focus");d(this).addClass("ui-state-focus")}}).blur(function(){d(this).removeClass("ui-state-focus")});this.handles.each(function(c){d(this).data("index.ui-slider-handle",c)});this.handles.keydown(function(c){var e=true,f=d(this).data("index.ui-slider-handle"),g,h,i;if(!a.options.disabled){switch(c.keyCode){case d.ui.keyCode.HOME:case d.ui.keyCode.END:case d.ui.keyCode.PAGE_UP:case d.ui.keyCode.PAGE_DOWN:case d.ui.keyCode.UP:case d.ui.keyCode.RIGHT:case d.ui.keyCode.DOWN:case d.ui.keyCode.LEFT:e= -false;if(!a._keySliding){a._keySliding=true;d(this).addClass("ui-state-active");g=a._start(c,f);if(g===false)return}break}i=a.options.step;g=a.options.values&&a.options.values.length?(h=a.values(f)):(h=a.value());switch(c.keyCode){case d.ui.keyCode.HOME:h=a._valueMin();break;case d.ui.keyCode.END:h=a._valueMax();break;case d.ui.keyCode.PAGE_UP:h=a._trimAlignValue(g+(a._valueMax()-a._valueMin())/5);break;case d.ui.keyCode.PAGE_DOWN:h=a._trimAlignValue(g-(a._valueMax()-a._valueMin())/5);break;case d.ui.keyCode.UP:case d.ui.keyCode.RIGHT:if(g=== -a._valueMax())return;h=a._trimAlignValue(g+i);break;case d.ui.keyCode.DOWN:case d.ui.keyCode.LEFT:if(g===a._valueMin())return;h=a._trimAlignValue(g-i);break}a._slide(c,f,h);return e}}).keyup(function(c){var e=d(this).data("index.ui-slider-handle");if(a._keySliding){a._keySliding=false;a._stop(c,e);a._change(c,e);d(this).removeClass("ui-state-active")}});this._refreshValue();this._animateOff=false},destroy:function(){this.handles.remove();this.range.remove();this.element.removeClass("ui-slider ui-slider-horizontal ui-slider-vertical ui-slider-disabled ui-widget ui-widget-content ui-corner-all").removeData("slider").unbind(".slider"); -this._mouseDestroy();return this},_mouseCapture:function(a){var b=this.options,c,e,f,g,h,i;if(b.disabled)return false;this.elementSize={width:this.element.outerWidth(),height:this.element.outerHeight()};this.elementOffset=this.element.offset();c={x:a.pageX,y:a.pageY};e=this._normValueFromMouse(c);f=this._valueMax()-this._valueMin()+1;h=this;this.handles.each(function(j){var k=Math.abs(e-h.values(j));if(f>k){f=k;g=d(this);i=j}});if(b.range===true&&this.values(1)===b.min){i+=1;g=d(this.handles[i])}if(this._start(a, -i)===false)return false;this._mouseSliding=true;h._handleIndex=i;g.addClass("ui-state-active").focus();b=g.offset();this._clickOffset=!d(a.target).parents().andSelf().is(".ui-slider-handle")?{left:0,top:0}:{left:a.pageX-b.left-g.width()/2,top:a.pageY-b.top-g.height()/2-(parseInt(g.css("borderTopWidth"),10)||0)-(parseInt(g.css("borderBottomWidth"),10)||0)+(parseInt(g.css("marginTop"),10)||0)};e=this._normValueFromMouse(c);this._slide(a,i,e);return this._animateOff=true},_mouseStart:function(){return true}, -_mouseDrag:function(a){var b=this._normValueFromMouse({x:a.pageX,y:a.pageY});this._slide(a,this._handleIndex,b);return false},_mouseStop:function(a){this.handles.removeClass("ui-state-active");this._mouseSliding=false;this._stop(a,this._handleIndex);this._change(a,this._handleIndex);this._clickOffset=this._handleIndex=null;return this._animateOff=false},_detectOrientation:function(){this.orientation=this.options.orientation==="vertical"?"vertical":"horizontal"},_normValueFromMouse:function(a){var b; -if(this.orientation==="horizontal"){b=this.elementSize.width;a=a.x-this.elementOffset.left-(this._clickOffset?this._clickOffset.left:0)}else{b=this.elementSize.height;a=a.y-this.elementOffset.top-(this._clickOffset?this._clickOffset.top:0)}b=a/b;if(b>1)b=1;if(b<0)b=0;if(this.orientation==="vertical")b=1-b;a=this._valueMax()-this._valueMin();return this._trimAlignValue(this._valueMin()+b*a)},_start:function(a,b){var c={handle:this.handles[b],value:this.value()};if(this.options.values&&this.options.values.length){c.value= -this.values(b);c.values=this.values()}return this._trigger("start",a,c)},_slide:function(a,b,c){var e;if(this.options.values&&this.options.values.length){e=this.values(b?0:1);if(this.options.values.length===2&&this.options.range===true&&(b===0&&c>e||b===1&&c1){this.options.values[a]=this._trimAlignValue(b);this._refreshValue();this._change(null,a)}if(arguments.length)if(d.isArray(arguments[0])){c=this.options.values;e=arguments[0];for(f=0;fthis._valueMax())return this._valueMax();var b=this.options.step>0?this.options.step:1,c=a%b;a=a-c;if(Math.abs(c)*2>=b)a+=c>0?b:-b;return parseFloat(a.toFixed(5))},_valueMin:function(){return this.options.min},_valueMax:function(){return this.options.max},_refreshValue:function(){var a= -this.options.range,b=this.options,c=this,e=!this._animateOff?b.animate:false,f,g={},h,i,j,k;if(this.options.values&&this.options.values.length)this.handles.each(function(l){f=(c.values(l)-c._valueMin())/(c._valueMax()-c._valueMin())*100;g[c.orientation==="horizontal"?"left":"bottom"]=f+"%";d(this).stop(1,1)[e?"animate":"css"](g,b.animate);if(c.options.range===true)if(c.orientation==="horizontal"){if(l===0)c.range.stop(1,1)[e?"animate":"css"]({left:f+"%"},b.animate);if(l===1)c.range[e?"animate":"css"]({width:f- -h+"%"},{queue:false,duration:b.animate})}else{if(l===0)c.range.stop(1,1)[e?"animate":"css"]({bottom:f+"%"},b.animate);if(l===1)c.range[e?"animate":"css"]({height:f-h+"%"},{queue:false,duration:b.animate})}h=f});else{i=this.value();j=this._valueMin();k=this._valueMax();f=k!==j?(i-j)/(k-j)*100:0;g[c.orientation==="horizontal"?"left":"bottom"]=f+"%";this.handle.stop(1,1)[e?"animate":"css"](g,b.animate);if(a==="min"&&this.orientation==="horizontal")this.range.stop(1,1)[e?"animate":"css"]({width:f+"%"}, -b.animate);if(a==="max"&&this.orientation==="horizontal")this.range[e?"animate":"css"]({width:100-f+"%"},{queue:false,duration:b.animate});if(a==="min"&&this.orientation==="vertical")this.range.stop(1,1)[e?"animate":"css"]({height:f+"%"},b.animate);if(a==="max"&&this.orientation==="vertical")this.range[e?"animate":"css"]({height:100-f+"%"},{queue:false,duration:b.animate})}}});d.extend(d.ui.slider,{version:"1.8.2"})})(jQuery); -;/* - * jQuery UI Tabs 1.8.2 - * - * Copyright (c) 2010 AUTHORS.txt (http://jqueryui.com/about) - * Dual licensed under the MIT (MIT-LICENSE.txt) - * and GPL (GPL-LICENSE.txt) licenses. - * - * http://docs.jquery.com/UI/Tabs - * - * Depends: - * jquery.ui.core.js - * jquery.ui.widget.js - */ -(function(d){function s(){return++u}function v(){return++w}var u=0,w=0;d.widget("ui.tabs",{options:{add:null,ajaxOptions:null,cache:false,cookie:null,collapsible:false,disable:null,disabled:[],enable:null,event:"click",fx:null,idPrefix:"ui-tabs-",load:null,panelTemplate:"
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-this.element.queue("tabs",function(){b._trigger("show",null,b._ui(b.anchors[0],b.panels[0]))});this.load(0)}this._trigger("add",null,this._ui(this.anchors[a],this.panels[a]));return this},remove:function(c){var e=this.options,a=this.lis.eq(c).remove(),b=this.panels.eq(c).remove();if(a.hasClass("ui-tabs-selected")&&this.anchors.length>1)this.select(c+(c+1=c?--h:h});this._tabify();this._trigger("remove", -null,this._ui(a.find("a")[0],b[0]));return this},enable:function(c){var e=this.options;if(d.inArray(c,e.disabled)!=-1){this.lis.eq(c).removeClass("ui-state-disabled");e.disabled=d.grep(e.disabled,function(a){return a!=c});this._trigger("enable",null,this._ui(this.anchors[c],this.panels[c]));return this}},disable:function(c){var e=this.options;if(c!=e.selected){this.lis.eq(c).addClass("ui-state-disabled");e.disabled.push(c);e.disabled.sort();this._trigger("disable",null,this._ui(this.anchors[c],this.panels[c]))}return this}, -select:function(c){if(typeof c=="string")c=this.anchors.index(this.anchors.filter("[href$="+c+"]"));else if(c===null)c=-1;if(c==-1&&this.options.collapsible)c=this.options.selected;this.anchors.eq(c).trigger(this.options.event+".tabs");return this},load:function(c){var e=this,a=this.options,b=this.anchors.eq(c)[0],h=d.data(b,"load.tabs");this.abort();if(!h||this.element.queue("tabs").length!==0&&d.data(b,"cache.tabs"))this.element.dequeue("tabs");else{this.lis.eq(c).addClass("ui-state-processing"); -if(a.spinner){var i=d("span",b);i.data("label.tabs",i.html()).html(a.spinner)}this.xhr=d.ajax(d.extend({},a.ajaxOptions,{url:h,success:function(k,n){d(e._sanitizeSelector(b.hash)).html(k);e._cleanup();a.cache&&d.data(b,"cache.tabs",true);e._trigger("load",null,e._ui(e.anchors[c],e.panels[c]));try{a.ajaxOptions.success(k,n)}catch(m){}},error:function(k,n){e._cleanup();e._trigger("load",null,e._ui(e.anchors[c],e.panels[c]));try{a.ajaxOptions.error(k,n,c,b)}catch(m){}}}));e.element.dequeue("tabs");return this}}, -abort:function(){this.element.queue([]);this.panels.stop(false,true);this.element.queue("tabs",this.element.queue("tabs").splice(-2,2));if(this.xhr){this.xhr.abort();delete this.xhr}this._cleanup();return this},url:function(c,e){this.anchors.eq(c).removeData("cache.tabs").data("load.tabs",e);return this},length:function(){return this.anchors.length}});d.extend(d.ui.tabs,{version:"1.8.2"});d.extend(d.ui.tabs.prototype,{rotation:null,rotate:function(c,e){var a=this,b=this.options,h=a._rotate||(a._rotate= -function(i){clearTimeout(a.rotation);a.rotation=setTimeout(function(){var k=b.selected;a.select(++k')}function E(a,b){d.extend(a, -b);for(var c in b)if(b[c]==null||b[c]==undefined)a[c]=b[c];return a}d.extend(d.ui,{datepicker:{version:"1.8.2"}});var y=(new Date).getTime();d.extend(J.prototype,{markerClassName:"hasDatepicker",log:function(){this.debug&&console.log.apply("",arguments)},_widgetDatepicker:function(){return this.dpDiv},setDefaults:function(a){E(this._defaults,a||{});return this},_attachDatepicker:function(a,b){var c=null;for(var e in this._defaults){var f=a.getAttribute("date:"+e);if(f){c=c||{};try{c[e]=eval(f)}catch(h){c[e]= -f}}}e=a.nodeName.toLowerCase();f=e=="div"||e=="span";if(!a.id){this.uuid+=1;a.id="dp"+this.uuid}var i=this._newInst(d(a),f);i.settings=d.extend({},b||{},c||{});if(e=="input")this._connectDatepicker(a,i);else f&&this._inlineDatepicker(a,i)},_newInst:function(a,b){return{id:a[0].id.replace(/([^A-Za-z0-9_])/g,"\\\\$1"),input:a,selectedDay:0,selectedMonth:0,selectedYear:0,drawMonth:0,drawYear:0,inline:b,dpDiv:!b?this.dpDiv:d('
    ')}}, -_connectDatepicker:function(a,b){var c=d(a);b.append=d([]);b.trigger=d([]);if(!c.hasClass(this.markerClassName)){this._attachments(c,b);c.addClass(this.markerClassName).keydown(this._doKeyDown).keypress(this._doKeyPress).keyup(this._doKeyUp).bind("setData.datepicker",function(e,f,h){b.settings[f]=h}).bind("getData.datepicker",function(e,f){return this._get(b,f)});this._autoSize(b);d.data(a,"datepicker",b)}},_attachments:function(a,b){var c=this._get(b,"appendText"),e=this._get(b,"isRTL");b.append&& -b.append.remove();if(c){b.append=d(''+c+"");a[e?"before":"after"](b.append)}a.unbind("focus",this._showDatepicker);b.trigger&&b.trigger.remove();c=this._get(b,"showOn");if(c=="focus"||c=="both")a.focus(this._showDatepicker);if(c=="button"||c=="both"){c=this._get(b,"buttonText");var f=this._get(b,"buttonImage");b.trigger=d(this._get(b,"buttonImageOnly")?d("").addClass(this._triggerClass).attr({src:f,alt:c,title:c}):d('').addClass(this._triggerClass).html(f== -""?c:d("").attr({src:f,alt:c,title:c})));a[e?"before":"after"](b.trigger);b.trigger.click(function(){d.datepicker._datepickerShowing&&d.datepicker._lastInput==a[0]?d.datepicker._hideDatepicker():d.datepicker._showDatepicker(a[0]);return false})}},_autoSize:function(a){if(this._get(a,"autoSize")&&!a.inline){var b=new Date(2009,11,20),c=this._get(a,"dateFormat");if(c.match(/[DM]/)){var e=function(f){for(var h=0,i=0,g=0;gh){h=f[g].length;i=g}return i};b.setMonth(e(this._get(a, -c.match(/MM/)?"monthNames":"monthNamesShort")));b.setDate(e(this._get(a,c.match(/DD/)?"dayNames":"dayNamesShort"))+20-b.getDay())}a.input.attr("size",this._formatDate(a,b).length)}},_inlineDatepicker:function(a,b){var c=d(a);if(!c.hasClass(this.markerClassName)){c.addClass(this.markerClassName).append(b.dpDiv).bind("setData.datepicker",function(e,f,h){b.settings[f]=h}).bind("getData.datepicker",function(e,f){return this._get(b,f)});d.data(a,"datepicker",b);this._setDate(b,this._getDefaultDate(b), -true);this._updateDatepicker(b);this._updateAlternate(b)}},_dialogDatepicker:function(a,b,c,e,f){a=this._dialogInst;if(!a){this.uuid+=1;this._dialogInput=d('');this._dialogInput.keydown(this._doKeyDown);d("body").append(this._dialogInput);a=this._dialogInst=this._newInst(this._dialogInput,false);a.settings={};d.data(this._dialogInput[0],"datepicker",a)}E(a.settings,e||{});b=b&&b.constructor== -Date?this._formatDate(a,b):b;this._dialogInput.val(b);this._pos=f?f.length?f:[f.pageX,f.pageY]:null;if(!this._pos)this._pos=[document.documentElement.clientWidth/2-100+(document.documentElement.scrollLeft||document.body.scrollLeft),document.documentElement.clientHeight/2-150+(document.documentElement.scrollTop||document.body.scrollTop)];this._dialogInput.css("left",this._pos[0]+20+"px").css("top",this._pos[1]+"px");a.settings.onSelect=c;this._inDialog=true;this.dpDiv.addClass(this._dialogClass);this._showDatepicker(this._dialogInput[0]); -d.blockUI&&d.blockUI(this.dpDiv);d.data(this._dialogInput[0],"datepicker",a);return this},_destroyDatepicker:function(a){var b=d(a),c=d.data(a,"datepicker");if(b.hasClass(this.markerClassName)){var e=a.nodeName.toLowerCase();d.removeData(a,"datepicker");if(e=="input"){c.append.remove();c.trigger.remove();b.removeClass(this.markerClassName).unbind("focus",this._showDatepicker).unbind("keydown",this._doKeyDown).unbind("keypress",this._doKeyPress).unbind("keyup",this._doKeyUp)}else if(e=="div"||e=="span")b.removeClass(this.markerClassName).empty()}}, -_enableDatepicker:function(a){var b=d(a),c=d.data(a,"datepicker");if(b.hasClass(this.markerClassName)){var e=a.nodeName.toLowerCase();if(e=="input"){a.disabled=false;c.trigger.filter("button").each(function(){this.disabled=false}).end().filter("img").css({opacity:"1.0",cursor:""})}else if(e=="div"||e=="span")b.children("."+this._inlineClass).children().removeClass("ui-state-disabled");this._disabledInputs=d.map(this._disabledInputs,function(f){return f==a?null:f})}},_disableDatepicker:function(a){var b= -d(a),c=d.data(a,"datepicker");if(b.hasClass(this.markerClassName)){var e=a.nodeName.toLowerCase();if(e=="input"){a.disabled=true;c.trigger.filter("button").each(function(){this.disabled=true}).end().filter("img").css({opacity:"0.5",cursor:"default"})}else if(e=="div"||e=="span")b.children("."+this._inlineClass).children().addClass("ui-state-disabled");this._disabledInputs=d.map(this._disabledInputs,function(f){return f==a?null:f});this._disabledInputs[this._disabledInputs.length]=a}},_isDisabledDatepicker:function(a){if(!a)return false; -for(var b=0;b-1}},_doKeyUp:function(a){a=d.datepicker._getInst(a.target);if(a.input.val()!=a.lastVal)try{if(d.datepicker.parseDate(d.datepicker._get(a,"dateFormat"),a.input?a.input.val():null,d.datepicker._getFormatConfig(a))){d.datepicker._setDateFromField(a);d.datepicker._updateAlternate(a);d.datepicker._updateDatepicker(a)}}catch(b){d.datepicker.log(b)}return true},_showDatepicker:function(a){a=a.target|| -a;if(a.nodeName.toLowerCase()!="input")a=d("input",a.parentNode)[0];if(!(d.datepicker._isDisabledDatepicker(a)||d.datepicker._lastInput==a)){var b=d.datepicker._getInst(a);d.datepicker._curInst&&d.datepicker._curInst!=b&&d.datepicker._curInst.dpDiv.stop(true,true);var c=d.datepicker._get(b,"beforeShow");E(b.settings,c?c.apply(a,[a,b]):{});b.lastVal=null;d.datepicker._lastInput=a;d.datepicker._setDateFromField(b);if(d.datepicker._inDialog)a.value="";if(!d.datepicker._pos){d.datepicker._pos=d.datepicker._findPos(a); -d.datepicker._pos[1]+=a.offsetHeight}var e=false;d(a).parents().each(function(){e|=d(this).css("position")=="fixed";return!e});if(e&&d.browser.opera){d.datepicker._pos[0]-=document.documentElement.scrollLeft;d.datepicker._pos[1]-=document.documentElement.scrollTop}c={left:d.datepicker._pos[0],top:d.datepicker._pos[1]};d.datepicker._pos=null;b.dpDiv.css({position:"absolute",display:"block",top:"-1000px"});d.datepicker._updateDatepicker(b);c=d.datepicker._checkOffset(b,c,e);b.dpDiv.css({position:d.datepicker._inDialog&& -d.blockUI?"static":e?"fixed":"absolute",display:"none",left:c.left+"px",top:c.top+"px"});if(!b.inline){c=d.datepicker._get(b,"showAnim");var f=d.datepicker._get(b,"duration"),h=function(){d.datepicker._datepickerShowing=true;var i=d.datepicker._getBorders(b.dpDiv);b.dpDiv.find("iframe.ui-datepicker-cover").css({left:-i[0],top:-i[1],width:b.dpDiv.outerWidth(),height:b.dpDiv.outerHeight()})};b.dpDiv.zIndex(d(a).zIndex()+1);d.effects&&d.effects[c]?b.dpDiv.show(c,d.datepicker._get(b,"showOptions"),f, -h):b.dpDiv[c||"show"](c?f:null,h);if(!c||!f)h();b.input.is(":visible")&&!b.input.is(":disabled")&&b.input.focus();d.datepicker._curInst=b}}},_updateDatepicker:function(a){var b=this,c=d.datepicker._getBorders(a.dpDiv);a.dpDiv.empty().append(this._generateHTML(a)).find("iframe.ui-datepicker-cover").css({left:-c[0],top:-c[1],width:a.dpDiv.outerWidth(),height:a.dpDiv.outerHeight()}).end().find("button, .ui-datepicker-prev, .ui-datepicker-next, .ui-datepicker-calendar td a").bind("mouseout",function(){d(this).removeClass("ui-state-hover"); -this.className.indexOf("ui-datepicker-prev")!=-1&&d(this).removeClass("ui-datepicker-prev-hover");this.className.indexOf("ui-datepicker-next")!=-1&&d(this).removeClass("ui-datepicker-next-hover")}).bind("mouseover",function(){if(!b._isDisabledDatepicker(a.inline?a.dpDiv.parent()[0]:a.input[0])){d(this).parents(".ui-datepicker-calendar").find("a").removeClass("ui-state-hover");d(this).addClass("ui-state-hover");this.className.indexOf("ui-datepicker-prev")!=-1&&d(this).addClass("ui-datepicker-prev-hover"); -this.className.indexOf("ui-datepicker-next")!=-1&&d(this).addClass("ui-datepicker-next-hover")}}).end().find("."+this._dayOverClass+" a").trigger("mouseover").end();c=this._getNumberOfMonths(a);var e=c[1];e>1?a.dpDiv.addClass("ui-datepicker-multi-"+e).css("width",17*e+"em"):a.dpDiv.removeClass("ui-datepicker-multi-2 ui-datepicker-multi-3 ui-datepicker-multi-4").width("");a.dpDiv[(c[0]!=1||c[1]!=1?"add":"remove")+"Class"]("ui-datepicker-multi");a.dpDiv[(this._get(a,"isRTL")?"add":"remove")+"Class"]("ui-datepicker-rtl"); -a==d.datepicker._curInst&&d.datepicker._datepickerShowing&&a.input&&a.input.is(":visible")&&!a.input.is(":disabled")&&a.input.focus()},_getBorders:function(a){var b=function(c){return{thin:1,medium:2,thick:3}[c]||c};return[parseFloat(b(a.css("border-left-width"))),parseFloat(b(a.css("border-top-width")))]},_checkOffset:function(a,b,c){var e=a.dpDiv.outerWidth(),f=a.dpDiv.outerHeight(),h=a.input?a.input.outerWidth():0,i=a.input?a.input.outerHeight():0,g=document.documentElement.clientWidth+d(document).scrollLeft(), -k=document.documentElement.clientHeight+d(document).scrollTop();b.left-=this._get(a,"isRTL")?e-h:0;b.left-=c&&b.left==a.input.offset().left?d(document).scrollLeft():0;b.top-=c&&b.top==a.input.offset().top+i?d(document).scrollTop():0;b.left-=Math.min(b.left,b.left+e>g&&g>e?Math.abs(b.left+e-g):0);b.top-=Math.min(b.top,b.top+f>k&&k>f?Math.abs(f+i):0);return b},_findPos:function(a){for(var b=this._get(this._getInst(a),"isRTL");a&&(a.type=="hidden"||a.nodeType!=1);)a=a[b?"previousSibling":"nextSibling"]; -a=d(a).offset();return[a.left,a.top]},_hideDatepicker:function(a){var b=this._curInst;if(!(!b||a&&b!=d.data(a,"datepicker")))if(this._datepickerShowing){a=this._get(b,"showAnim");var c=this._get(b,"duration"),e=function(){d.datepicker._tidyDialog(b);this._curInst=null};d.effects&&d.effects[a]?b.dpDiv.hide(a,d.datepicker._get(b,"showOptions"),c,e):b.dpDiv[a=="slideDown"?"slideUp":a=="fadeIn"?"fadeOut":"hide"](a?c:null,e);a||e();if(a=this._get(b,"onClose"))a.apply(b.input?b.input[0]:null,[b.input?b.input.val(): -"",b]);this._datepickerShowing=false;this._lastInput=null;if(this._inDialog){this._dialogInput.css({position:"absolute",left:"0",top:"-100px"});if(d.blockUI){d.unblockUI();d("body").append(this.dpDiv)}}this._inDialog=false}},_tidyDialog:function(a){a.dpDiv.removeClass(this._dialogClass).unbind(".ui-datepicker-calendar")},_checkExternalClick:function(a){if(d.datepicker._curInst){a=d(a.target);a[0].id!=d.datepicker._mainDivId&&a.parents("#"+d.datepicker._mainDivId).length==0&&!a.hasClass(d.datepicker.markerClassName)&& -!a.hasClass(d.datepicker._triggerClass)&&d.datepicker._datepickerShowing&&!(d.datepicker._inDialog&&d.blockUI)&&d.datepicker._hideDatepicker()}},_adjustDate:function(a,b,c){a=d(a);var e=this._getInst(a[0]);if(!this._isDisabledDatepicker(a[0])){this._adjustInstDate(e,b+(c=="M"?this._get(e,"showCurrentAtPos"):0),c);this._updateDatepicker(e)}},_gotoToday:function(a){a=d(a);var b=this._getInst(a[0]);if(this._get(b,"gotoCurrent")&&b.currentDay){b.selectedDay=b.currentDay;b.drawMonth=b.selectedMonth=b.currentMonth; -b.drawYear=b.selectedYear=b.currentYear}else{var c=new Date;b.selectedDay=c.getDate();b.drawMonth=b.selectedMonth=c.getMonth();b.drawYear=b.selectedYear=c.getFullYear()}this._notifyChange(b);this._adjustDate(a)},_selectMonthYear:function(a,b,c){a=d(a);var e=this._getInst(a[0]);e._selectingMonthYear=false;e["selected"+(c=="M"?"Month":"Year")]=e["draw"+(c=="M"?"Month":"Year")]=parseInt(b.options[b.selectedIndex].value,10);this._notifyChange(e);this._adjustDate(a)},_clickMonthYear:function(a){a=this._getInst(d(a)[0]); -a.input&&a._selectingMonthYear&&!d.browser.msie&&a.input.focus();a._selectingMonthYear=!a._selectingMonthYear},_selectDay:function(a,b,c,e){var f=d(a);if(!(d(e).hasClass(this._unselectableClass)||this._isDisabledDatepicker(f[0]))){f=this._getInst(f[0]);f.selectedDay=f.currentDay=d("a",e).html();f.selectedMonth=f.currentMonth=b;f.selectedYear=f.currentYear=c;this._selectDate(a,this._formatDate(f,f.currentDay,f.currentMonth,f.currentYear))}},_clearDate:function(a){a=d(a);this._getInst(a[0]);this._selectDate(a, -"")},_selectDate:function(a,b){a=this._getInst(d(a)[0]);b=b!=null?b:this._formatDate(a);a.input&&a.input.val(b);this._updateAlternate(a);var c=this._get(a,"onSelect");if(c)c.apply(a.input?a.input[0]:null,[b,a]);else a.input&&a.input.trigger("change");if(a.inline)this._updateDatepicker(a);else{this._hideDatepicker();this._lastInput=a.input[0];typeof a.input[0]!="object"&&a.input.focus();this._lastInput=null}},_updateAlternate:function(a){var b=this._get(a,"altField");if(b){var c=this._get(a,"altFormat")|| -this._get(a,"dateFormat"),e=this._getDate(a),f=this.formatDate(c,e,this._getFormatConfig(a));d(b).each(function(){d(this).val(f)})}},noWeekends:function(a){a=a.getDay();return[a>0&&a<6,""]},iso8601Week:function(a){a=new Date(a.getTime());a.setDate(a.getDate()+4-(a.getDay()||7));var b=a.getTime();a.setMonth(0);a.setDate(1);return Math.floor(Math.round((b-a)/864E5)/7)+1},parseDate:function(a,b,c){if(a==null||b==null)throw"Invalid arguments";b=typeof b=="object"?b.toString():b+"";if(b=="")return null; -for(var e=(c?c.shortYearCutoff:null)||this._defaults.shortYearCutoff,f=(c?c.dayNamesShort:null)||this._defaults.dayNamesShort,h=(c?c.dayNames:null)||this._defaults.dayNames,i=(c?c.monthNamesShort:null)||this._defaults.monthNamesShort,g=(c?c.monthNames:null)||this._defaults.monthNames,k=c=-1,l=-1,u=-1,j=false,o=function(p){(p=z+1-1){k=1;l=u;do{e=this._getDaysInMonth(c,k-1);if(l<=e)break;k++;l-=e}while(1)}v=this._daylightSavingAdjust(new Date(c, -k-1,l));if(v.getFullYear()!=c||v.getMonth()+1!=k||v.getDate()!=l)throw"Invalid date";return v},ATOM:"yy-mm-dd",COOKIE:"D, dd M yy",ISO_8601:"yy-mm-dd",RFC_822:"D, d M y",RFC_850:"DD, dd-M-y",RFC_1036:"D, d M y",RFC_1123:"D, d M yy",RFC_2822:"D, d M yy",RSS:"D, d M y",TICKS:"!",TIMESTAMP:"@",W3C:"yy-mm-dd",_ticksTo1970:(718685+Math.floor(492.5)-Math.floor(19.7)+Math.floor(4.925))*24*60*60*1E7,formatDate:function(a,b,c){if(!b)return"";var e=(c?c.dayNamesShort:null)||this._defaults.dayNamesShort,f=(c? -c.dayNames:null)||this._defaults.dayNames,h=(c?c.monthNamesShort:null)||this._defaults.monthNamesShort;c=(c?c.monthNames:null)||this._defaults.monthNames;var i=function(o){(o=j+112?a.getHours()+2:0);return a},_setDate:function(a,b,c){var e=!b,f=a.selectedMonth,h=a.selectedYear;b=this._restrictMinMax(a,this._determineDate(a,b,new Date));a.selectedDay=a.currentDay=b.getDate();a.drawMonth=a.selectedMonth=a.currentMonth=b.getMonth();a.drawYear=a.selectedYear=a.currentYear=b.getFullYear();if((f!=a.selectedMonth||h!=a.selectedYear)&&!c)this._notifyChange(a);this._adjustInstDate(a);if(a.input)a.input.val(e?"":this._formatDate(a))},_getDate:function(a){return!a.currentYear|| -a.input&&a.input.val()==""?null:this._daylightSavingAdjust(new Date(a.currentYear,a.currentMonth,a.currentDay))},_generateHTML:function(a){var b=new Date;b=this._daylightSavingAdjust(new Date(b.getFullYear(),b.getMonth(),b.getDate()));var c=this._get(a,"isRTL"),e=this._get(a,"showButtonPanel"),f=this._get(a,"hideIfNoPrevNext"),h=this._get(a,"navigationAsDateFormat"),i=this._getNumberOfMonths(a),g=this._get(a,"showCurrentAtPos"),k=this._get(a,"stepMonths"),l=i[0]!=1||i[1]!=1,u=this._daylightSavingAdjust(!a.currentDay? -new Date(9999,9,9):new Date(a.currentYear,a.currentMonth,a.currentDay)),j=this._getMinMaxDate(a,"min"),o=this._getMinMaxDate(a,"max");g=a.drawMonth-g;var m=a.drawYear;if(g<0){g+=12;m--}if(o){var n=this._daylightSavingAdjust(new Date(o.getFullYear(),o.getMonth()-i[0]*i[1]+1,o.getDate()));for(n=j&&nn;){g--;if(g<0){g=11;m--}}}a.drawMonth=g;a.drawYear=m;n=this._get(a,"prevText");n=!h?n:this.formatDate(n,this._daylightSavingAdjust(new Date(m,g-k,1)),this._getFormatConfig(a)); -n=this._canAdjustMonth(a,-1,m,g)?''+n+"":f?"":''+n+"";var r=this._get(a,"nextText");r=!h?r:this.formatDate(r,this._daylightSavingAdjust(new Date(m, -g+k,1)),this._getFormatConfig(a));f=this._canAdjustMonth(a,+1,m,g)?''+r+"":f?"":''+r+"";k=this._get(a,"currentText");r=this._get(a,"gotoCurrent")&& -a.currentDay?u:b;k=!h?k:this.formatDate(k,r,this._getFormatConfig(a));h=!a.inline?'":"";e=e?'
    '+(c?h:"")+(this._isInRange(a,r)?'":"")+(c?"":h)+"
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z9rbu+Z-Hr=BL#6CjylI-Gla6#!B@;CW(7#}GZvR5z2gndPX8%>N%~BgDdnPj7w~6j z6q>v+ed^I&aCS**&Z4hp~@_F4QO5!v2E&>Uc2sn>bXk9vV!_5F7i)<5c>II?$Q zdYS~scy^|yN$p7*#)+q9XL2d?YUg=VHCszn^Qj45QvTwO{=hOZo+4?Y+~s#k+J%4X z!ZFj{wGD!g)^B*}#4jQVRiPrFai=XlWBrA$;AQ=5NMq8mdDXYJW8O8((Pxjx`Rrk?*IJgAl|H%tC!9H#* 0 ? this.model.reports.at(0).toJSON() : null, "progress_flows": (status == "Complete" ? this.model.get('flows') : status), @@ -299,6 +303,10 @@ $(function () { }, toggle_star: function () { + if(this.model.get("_host")){ + alert("Runs can only be starred on thier originating Torrent Server."); + return; + } if (this.model.get("star")) { this.model.patch({star: false}); } else { @@ -307,6 +315,10 @@ $(function () { }, change_representative: function(report) { + if(this.model.get("_host")){ + alert("Reports can only be marked as representative on thier originating Torrent Server."); + return; + } console.log("Toggling experiment"); var url = report.url() + 'representative/'; var data; @@ -403,7 +415,8 @@ $(function () { 'click .edit-run': 'edit' }, - initialize: function () { + initialize: function (options) { + this.options = options; _.bindAll(this, 'render', 'destroy_view', 'toggle_star', 'edit'); this.model.bind('change', this.render); this.model.bind('remove', this.destroy_view); @@ -422,20 +435,28 @@ $(function () { url_prefix = "http://" + this.model.get('_host'); exp_name_prefix = "" + this.model.get('_host') + " " } + var other_reports = this.model.reports.map(function (report) { + return report.toJSON() + }).filter(function (report) { + if (king_report && king_report.id == report.id) { + return false; + } + return true; + }); display_host = this.$el.html(this.template.render({ "exp": this.model.toJSON(), "is_local": is_local, //Mesh "url_prefix": url_prefix, //Mesh "exp_name_prefix": exp_name_prefix, //Mesh - "run_date_string": this.model.get('date').toString("MM/dd/yy"), - "result_date_string": this.model.get('resultDate').toString("MM/dd/yy"), + "run_date_string": this.model.get('date').toString("MMM d yyyy"), + "result_date_string": this.model.get('resultDate').toString("MMM d yyyy"), "king_report": king_report, "progress_flows": (status == "Complete" ? this.model.get('flows') : status), "progress_percent": status == "Complete" ? 100 : Math.round((status / this.model.get('flows')) * 100), "in_progress": !isNaN(parseInt(status)), "total_q20bp": function () { - return king_report && king_report.quality_metrics && precisionUnits(king_report.quality_metrics.q20_bases); + return (king_report && king_report.isShowAllMetrics) ? king_report.quality_metrics && precisionUnits(king_report.quality_metrics.q20_bases) : "---"; }, "total_q0bp": function () { return king_report && king_report.quality_metrics && precisionUnits(king_report.quality_metrics.q0_bases); @@ -445,11 +466,14 @@ $(function () { }, "read_length": function () { return king_report && king_report.quality_metrics && precisionUnits(king_report.quality_metrics.q0_mean_read_length); - } + }, + "other_reports": other_reports, + "has_other_reports": other_reports.length > 0, + more_columns: this.options.more_columns })); var samples = this.$el.children('.samples') samples.html(this.sample_template(this.model.toJSON())); - samples.find('[rel=popover]').popover({content: samples.find('#sample' + this.model.id).html()}); + samples.find('[rel=popover]').popover({content: samples.find(jqid('sample' + this.model.id)).html()}); }, edit: function (e) { @@ -492,11 +516,14 @@ $(function () { events: { 'change .search-field': 'search', 'click #search_text_go': 'search', + 'keypress #search_text' : 'check_search_key', 'click #clear_filters': 'clear_filters', 'click #live_button': 'toggle_live_update', 'click #download_csv': 'csv_download', 'click .sort_link': 'sort', - 'change #id_data_source': 'data_source_update' + 'change #id_data_source': 'data_source_update', + 'click #toggle_more_filters': 'toggle_more_filters', + 'click #toggle_more_columns': 'toggle_more_columns' }, initialize: function () { @@ -508,7 +535,18 @@ $(function () { $(".chzn-select").chosen({no_results_text:"No results matched", "allow_single_deselect":true}); $('.chzn-drop').css('width', $(".chzn-select").outerWidth()-2); //Patched per https://github.com/harvesthq/chosen/issues/453#issuecomment-8884310 $('.chzn-search input').css('width', $(".chzn-select").outerWidth()*.815); //Patched per https://github.com/harvesthq/chosen/issues/453#issuecomment-8884310 - $('#rangeA').daterangepicker({dateFormat: 'mm/dd/yy'}); + today = Date.parse('today'); + now = new Date(); + $('#rangeA').daterangepicker({ + dateFormat: 'M d yy', + presetRanges: [ + {text: 'Today', dateStart: today, dateEnd: today}, + {text: 'Last 7 Days', dateStart: 'today-7days', dateEnd: today}, + {text: 'Last 30 Days', dateStart: 'today-30days', dateEnd: today}, + {text: 'Last 60 Days', dateStart: 'today-60days', dateEnd: today}, + {text: 'Last 90 Days', dateStart: 'today-90days', dateEnd: today} + ], + }); this.current_view = null; this.collection.bind('add', this.addRun); this.collection.bind('reset', this.render); @@ -519,7 +557,96 @@ $(function () { this.router.on("route:full_view", this.view_full); this.router.on("route:table_view", this.view_table); this.live_update = null; + + // Load the more_* attributes in html storage + this.more_filters = false; + if (localStorage.getItem('data-more-filters') == "true") { + this.toggle_more_filters(); + } + this.more_columns = false; + if (localStorage.getItem('data-more-columns') == "true") { + this.toggle_more_columns(); + } + + this.isLocalDataSource = true; + this.updateSortIndicators(); //this.countdown_update(); + this.meshValues = null; + this.prefetchMeshValues(); + this.meshDropdowns = [ + {element: $("#id_project"), key: "projects"}, + {element: $("#id_sample"), key: "samples"}, + {element: $("#id_pgm"), key: "rigs"}, + {element: $("#id_reference"), key: "references"}, + ]; + + // If there are form fields with values loaded from the browser cache, we need to re fetch the table data + if (!$.isEmptyObject(this._get_query())) { + console.log("Search fields are not empty on page load. Running search."); + this.search(); + } + + }, + + prefetchMeshValues: function () { + $.ajax({ + url: "/rundb/api/mesh/v1/meshprefetch/", + dataType: "json" + }).done(function (response) { + //Save values + this.meshValues = response.values; + //Side effect to show error message + if (response.warnings && response.warnings.length > 0) { + response.warnings.map(function (warningText) { + console.log(warningText) + }); + } + //Enable mesh dropdown if we have any mesh nodes + var haveCompatibleNodes = false; + Object.keys(response.nodes).map(function (host) { + var optionElement = $("

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