Error in Input file

[Mahmoudbassuoni]

Hi I am trying to run the UpsetR to get the intersection among 6 lists. I have got my input as csv file with the first row as the sets names and the rest are designed to look like that:

when ever I run something like this upset(data = positions,nsets = 6,sets = c("EGYREF","AFR","AMR","EAS","EUR","SAS"), set.metadata = colnames(positions),show.numbers = TRUE,empty.intersections = TRUE)

I get the error : Error in start_col:end_col : argument of length 0

I don't know what could be causing the issue, I would appreciate if you can help! @JakeConway
NB. the length of all the columns are not equal as there are some of them who has entries more than the other and obvioulsy I need to intersect them all...