diff --git a/.changeset/tough-spies-tease.md b/.changeset/tough-spies-tease.md
new file mode 100644
index 0000000000..5be816c5e8
--- /dev/null
+++ b/.changeset/tough-spies-tease.md
@@ -0,0 +1,8 @@
+---
+"@nextui-org/autocomplete": patch
+"@nextui-org/dropdown": patch
+"@nextui-org/popover": patch
+"@nextui-org/tooltip": patch
+---
+
+fix(autocomplete): support isReadOnly for dynamic collections in Autocomplete
diff --git a/packages/components/autocomplete/src/use-autocomplete.ts b/packages/components/autocomplete/src/use-autocomplete.ts
index 2e688057de..65052b4ae3 100644
--- a/packages/components/autocomplete/src/use-autocomplete.ts
+++ b/packages/components/autocomplete/src/use-autocomplete.ts
@@ -163,15 +163,12 @@ export function useAutocomplete<T extends object>(originalProps: UseAutocomplete
   // Setup filter function and state.
   const {contains} = useFilter(filterOptions);
 
-  const state = useComboBoxState({
+  let state = useComboBoxState({
     ...originalProps,
     children,
     menuTrigger,
     shouldCloseOnBlur,
     allowsEmptyCollection,
-    ...(isReadOnly && {
-      disabledKeys: (children as unknown as Record<string, any>[]).map((o) => o.key),
-    }),
     defaultFilter: defaultFilter && typeof defaultFilter === "function" ? defaultFilter : contains,
     onOpenChange: (open, menuTrigger) => {
       onOpenChange?.(open, menuTrigger);
@@ -181,6 +178,13 @@ export function useAutocomplete<T extends object>(originalProps: UseAutocomplete
     },
   });
 
+  state = {
+    ...state,
+    ...(isReadOnly && {
+      disabledKeys: new Set([...state.collection.getKeys()].map((k) => k)),
+    }),
+  };
+
   // Setup refs and get props for child elements.
   const buttonRef = useRef<HTMLButtonElement>(null);
   const inputWrapperRef = useRef<HTMLDivElement>(null);