Release 1.14

Author: daviesrob

.cirrus.yml

@@ -48,7 +48,7 @@ test_template: &TEST
   test_script: |
     make test-shlib-exports
     make test
-
+    if test "x$DO_UNTRACKED_FILE_CHECK" = "xyes"; then make check-untracked ; fi
 
 #--------------------------------------------------
 # Task: linux builds.
@@ -68,6 +68,7 @@ gcc_task:
   matrix:
     - environment:
        DO_MAINTAINER_CHECKS: yes
+       DO_UNTRACKED_FILE_CHECK: yes
        USE_CONFIG: no
     - environment:
        USE_CONFIG: yes
@@ -96,6 +97,7 @@ ubuntu_task:
   matrix:
     - environment:
        USE_CONFIG: yes
+       DO_UNTRACKED_FILE_CHECK: yes
     - environment:
        USE_CONFIG: yes
        CFLAGS: -g -Wall -O3 -fsanitize=address

.gitignore

@@ -49,6 +49,7 @@ shlib-exports-*.txt
 /test/hts_endian
 /test/longrefs/*.tmp.*
 /test/pileup
+/test/pileup_mod
 /test/plugins-dlhts
 /test/sam
 /test/tabix/*.tmp.*
@@ -61,6 +62,7 @@ shlib-exports-*.txt
 /test/test_introspection
 /test/test_kfunc
 /test/test_kstring
+/test/test_mod
 /test/test-parse-reg
 /test/test_realn
 /test/test-regidx

Makefile

@@ -74,12 +74,14 @@ BUILT_TEST_PROGRAMS = \
 	test/fieldarith \
 	test/hfile \
 	test/pileup \
+	test/pileup_mod \
 	test/plugins-dlhts \
 	test/sam \
 	test/test_bgzf \
 	test/test_expr \
 	test/test_kfunc \
 	test/test_kstring \
+	test/test_mod \
 	test/test_realn \
 	test/test-regidx \
 	test/test_str2int \
@@ -129,8 +131,8 @@ LIBHTS_SOVERSION = 3
 # is not strictly necessary and should be removed the next time
 # LIBHTS_SOVERSION is bumped (see #1144 and
 # https://developer.apple.com/library/archive/documentation/DeveloperTools/Conceptual/DynamicLibraries/100-Articles/DynamicLibraryDesignGuidelines.html#//apple_ref/doc/uid/TP40002013-SW23)
-MACH_O_COMPATIBILITY_VERSION = 3.1.13
-MACH_O_CURRENT_VERSION = 3.1.13
+MACH_O_COMPATIBILITY_VERSION = 3.1.14
+MACH_O_CURRENT_VERSION = 3.1.14
 
 # $(NUMERIC_VERSION) is for items that must have a numeric X.Y.Z string
 # even if this is a dirty or untagged Git working tree.
@@ -363,7 +365,7 @@ hfile_gcs.o hfile_gcs.pico: hfile_gcs.c config.h $(htslib_hts_h) $(htslib_kstrin
 hfile_libcurl.o hfile_libcurl.pico: hfile_libcurl.c config.h $(hfile_internal_h) $(htslib_hts_h) $(htslib_kstring_h) $(htslib_khash_h)
 hfile_s3_write.o hfile_s3_write.pico: hfile_s3_write.c config.h $(hfile_internal_h) $(htslib_hts_h) $(htslib_kstring_h) $(htslib_khash_h)
 hfile_s3.o hfile_s3.pico: hfile_s3.c config.h $(hfile_internal_h) $(htslib_hts_h) $(htslib_kstring_h)
-hts.o hts.pico: hts.c config.h $(htslib_hts_expr_h) $(htslib_hts_h) $(htslib_bgzf_h) $(cram_h) $(htslib_hfile_h) $(htslib_hts_endian_h) version.h config_vars.h $(hts_internal_h) $(hfile_internal_h) $(sam_internal_h) $(htslib_hts_os_h) $(htslib_khash_h) $(htslib_kseq_h) $(htslib_ksort_h) $(htslib_tbx_h) $(htscodecs_htscodecs_h)
+hts.o hts.pico: hts.c config.h os/lzma_stub.h $(htslib_hts_h) $(htslib_bgzf_h) $(cram_h) $(htslib_hfile_h) $(htslib_hts_endian_h) version.h config_vars.h $(hts_internal_h) $(hfile_internal_h) $(sam_internal_h) $(htslib_hts_expr_h) $(htslib_hts_os_h) $(htslib_khash_h) $(htslib_kseq_h) $(htslib_ksort_h) $(htslib_tbx_h) $(htscodecs_htscodecs_h)
 hts_expr.o hts_expr.pico: hts_expr.c config.h $(htslib_hts_expr_h) $(textutils_internal_h)
 hts_os.o hts_os.pico: hts_os.c config.h $(htslib_hts_defs_h) os/rand.c
 vcf.o vcf.pico: vcf.c config.h $(htslib_vcf_h) $(htslib_bgzf_h) $(htslib_tbx_h) $(htslib_hfile_h) $(hts_internal_h) $(htslib_khash_str2int_h) $(htslib_kstring_h) $(htslib_sam_h) $(htslib_khash_h) $(htslib_kseq_h) $(htslib_hts_endian_h)
@@ -471,6 +473,13 @@ maintainer-check:
 	test/maintainer/check_copyright.pl .
 	test/maintainer/check_spaces.pl .
 
+# Look for untracked files in the git repository.
+check-untracked:
+	@if test -e .git && git status --porcelain | grep '^\?'; then \
+	    echo 'Untracked files detected (see above). Please either clean up, add to .gitignore, or for test output files consider naming them to match *.tmp or *.tmp.*' ; \
+	    false ; \
+	fi
+
 # Create a shorthand. We use $(SRC) or $(srcprefix) rather than $(srcdir)/
 # for brevity in test and install rules, and so that build logs do not have
 # ./ sprinkled throughout.
@@ -497,6 +506,7 @@ check test: $(BUILT_PROGRAMS) $(BUILT_TEST_PROGRAMS) $(BUILT_PLUGINS) $(HTSCODEC
 	cd test/tabix && ./test-tabix.sh tabix.tst
 	cd test/mpileup && ./test-pileup.sh mpileup.tst
 	cd test/fastq && ./test-fastq.sh
+	cd test/base_mods && ./base-mods.sh base-mods.tst
 	REF_PATH=: test/sam test/ce.fa test/faidx.fa test/fastqs.fq
 	test/test-regidx
 	cd test && REF_PATH=: ./test.pl $${TEST_OPTS:-}
@@ -516,6 +526,9 @@ test/hfile: test/hfile.o libhts.a
 test/pileup: test/pileup.o libhts.a
 	$(CC) $(LDFLAGS) -o $@ test/pileup.o libhts.a $(LIBS) -lpthread
 
+test/pileup_mod: test/pileup_mod.o libhts.a
+	$(CC) $(LDFLAGS) -o $@ test/pileup_mod.o libhts.a $(LIBS) -lpthread
+
 test/plugins-dlhts: test/plugins-dlhts.o
 	$(CC) $(LDFLAGS) -o $@ test/plugins-dlhts.o $(LIBS)
 
@@ -534,6 +547,9 @@ test/test_kfunc: test/test_kfunc.o libhts.a
 test/test_kstring: test/test_kstring.o libhts.a
 	$(CC) $(LDFLAGS) -o $@ test/test_kstring.o libhts.a -lz $(LIBS) -lpthread
 
+test/test_mod: test/test_mod.o libhts.a
+	$(CC) $(LDFLAGS) -o $@ test/test_mod.o libhts.a $(LIBS) -lpthread
+
 test/test_realn: test/test_realn.o libhts.a
 	$(CC) $(LDFLAGS) -o $@ test/test_realn.o libhts.a $(LIBS) -lpthread
 
@@ -622,12 +638,14 @@ test/fuzz/hts_open_fuzzer.o: test/fuzz/hts_open_fuzzer.c config.h $(htslib_hfile
 test/fieldarith.o: test/fieldarith.c config.h $(htslib_sam_h)
 test/hfile.o: test/hfile.c config.h $(htslib_hfile_h) $(htslib_hts_defs_h) $(htslib_kstring_h)
 test/pileup.o: test/pileup.c config.h $(htslib_sam_h) $(htslib_kstring_h)
+test/pileup_mod.o: test/pileup_mod.c config.h $(htslib_sam_h)
 test/plugins-dlhts.o: test/plugins-dlhts.c config.h
 test/sam.o: test/sam.c config.h $(htslib_hts_defs_h) $(htslib_sam_h) $(htslib_faidx_h) $(htslib_khash_h) $(htslib_hts_log_h)
 test/test_bgzf.o: test/test_bgzf.c config.h $(htslib_bgzf_h) $(htslib_hfile_h) $(hfile_internal_h)
 test/test_expr.o: test/test_expr.c config.h $(htslib_hts_expr_h)
 test/test_kfunc.o: test/test_kfunc.c config.h $(htslib_kfunc_h)
 test/test_kstring.o: test/test_kstring.c config.h $(htslib_kstring_h)
+test/test_mod.o: test/test_mod.c config.h $(htslib_sam_h)
 test/test-parse-reg.o: test/test-parse-reg.c config.h $(htslib_hts_h) $(htslib_sam_h)
 test/test_realn.o: test/test_realn.c config.h $(htslib_hts_h) $(htslib_sam_h) $(htslib_faidx_h)
 test/test-regidx.o: test/test-regidx.c config.h $(htslib_kstring_h) $(htslib_regidx_h) $(htslib_hts_defs_h) $(textutils_internal_h)
@@ -790,7 +808,7 @@ distdir:
 force:
 
 
-.PHONY: all check clean distclean distdir force
+.PHONY: all check check-untracked clean distclean distdir force
 .PHONY: install install-pkgconfig installdirs lib-shared lib-static
 .PHONY: maintainer-check maintainer-clean mostlyclean plugins
 .PHONY: print-config print-version show-version tags

NEWS

@@ -1,3 +1,96 @@
+Noteworthy changes in release 1.14 (22nd October 2021)
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+
+Features and Updates
+--------------------
+
+* Added a keep option to bgzip to leave the original file untouched.  This
+  brings bgzip into line with gzip. (PR #1331, thanks to Alex Petty)
+
+* "endpos" has been added to the filter language, giving the position
+  of the rightmost mapped base as measured by the CIGAR string.  For
+  unmapped reads it is the same as "pos". (PR #1307, thanks to John Marshall)
+
+* Interfaces have been added to interpret the new base modification tags
+  added to the SAMtags document in samtools/hts-specs#418.  (PR #1132)
+
+* New API functions hts_flush()/sam_flush()/bcf_flush() for flushing output
+  htsFile/samFile/vcfFile streams. (PR #1326, thanks to John Marshall)
+
+* The synced_bcf_reader now sorts lines with symbolic alleles by END tag as
+  well as POS.  (PR #1321)
+
+* Added synced_bcf_reader options BCF_SR_REGIONS_OVERLAP and
+  BCF_SR_TARGETS_OVERLAP for better control of records that start outside
+  the desired region but overlap it are handled.  Fixes samtools/bcftools#1420
+  and samtools/bcftools#1421 raised by John Marshall.  (PR #1327)
+
+* HTSlib will now accept long-cigar CG:B: tags made by htsjdk which don't
+  quite follow the specification properly (using signed values instead of
+  unsigned).  Thanks to Colin Diesh for reporting an example file. (PR #1317)
+
+* The warning printed when the BGZF reader finds a file with no EOF block
+  has been changed to be less alarming.  Unfortunately some third-party
+  BGZF encoders don't write EOF blocks at the end of files.  Thanks to
+  Keiran Raine for reporting an example file.  (PR #1323)
+
+* The FASTA and FASTQ readers get an option to skip over the first item on
+  the header line, and use the second as the read name.  It allows the original
+  name to be restored on some of the fastq files served from the European
+  Nucleotide Archive (ENA).  (PR #1325)
+
+* HTSlib is now more strict when parsing the VCF samples line (beginning
+  #CHROM).  It will only accept tabs between the mandatory field names and
+  sample names must be separated with tabs. (PR #1328)
+
+* HTSlib will now warn if it looks like the header has been corrupted
+  by diagnostic messages from the program that made it.  This can happen when
+  using `nohup`, which by default mixes stdout and stderr into the same
+  stream.  (PR#1339, thanks to John Marshall)
+
+* File format detection will now recognise signatures for XZ, Zstd and D4
+  files (note that HTSlib will not read them yet).  (PR #1340, thanks to
+  John Marshall)
+
+Build changes
+-------------
+
+These are compiler, configuration and makefile based changes.
+
+* Some redundant tests have been removed from the test harness, speeding it up.
+  (PR #1308)
+
+* The version.sh script now works better on shallow checkouts.  (PR #1324)
+
+* A check-untracked Makefile target has been added to catch untracked files
+  (mostly) left by the test harness.  (PR #1324)
+
+Bug fixes
+---------
+
+* Fixed a case where flushing the thread pool could very occasionally cause
+  a deadlock.  (PR #1309)
+
+* Fixed a bug where some CRAM files could fail to decode if the required_fields
+  option was in use.  Thanks to Matt Sexton for reporting the issue.
+  (PR #1314, fixes samtools/samtools#1475)
+
+* Fixed a regression where the S3 plugin could not read public files unless
+  you supplied some Amazon credentials.  Thanks to Chris Saunders for reporting.
+  (PR #1332, fixes samtools/samtools#1491)
+
+* Fixed a possible CRAM thread deadlock discovered by @ryancaicse.
+  (PR #1330, fixes #1329)
+
+* Some set-but-unused variables have been removed.  (PR #1334)
+
+* Fixed a bug which prevented "flag.read2" from working in the filter
+  language unless it was at the end of the expression.  Thanks to Vamsi Kodali
+  for reporting the issue.  (PR #1342)
+
+* Fixed a memory leak that could happen if CRAM fails to inflate a LZMA
+  block. (PR #1340, thanks to John Marshall)
+
 Noteworthy changes in release 1.13 (7th July 2021)
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
 
@@ -519,7 +612,7 @@ Bug fixes
 
 * Fixed potential integer overflows in the VCF parser and ensured that
   the total length of FORMAT fields cannot go over 2Gbytes. [fuzz] (#1044,
-  #1104)
+  #1104; latter is CVE-2020-36403 affecting HTSlib versions 1.10 to 1.10.2)
 
 * Download index files atomically in idx_test_and_fetch().  This prevents
   corruption when running parallel jobs on S3 files.  Thanks to John Marshall.

README.md

@@ -12,7 +12,7 @@ HTSlib implements a generalized BAM index, with file extension `.csi`
 (coordinate-sorted index). The HTSlib file reader first looks for the new index
 and then for the old if the new index is absent.
 
-This project also includes the popular tabix indexer, which indexes both `.tbi`
+This project also includes the popular tabix indexer, which creates both `.tbi`
 and `.csi` formats, and the bgzip compression utility.
 
 [1]: http://samtools.github.io/hts-specs/

bcf_sr_sort.c

@@ -1,5 +1,5 @@
 /*
-    Copyright (C) 2017-2019 Genome Research Ltd.
+    Copyright (C) 2017-2021 Genome Research Ltd.
 
     Author: Petr Danecek <[email protected]>
 
@@ -259,6 +259,7 @@ static int cmpstringp(const void *p1, const void *p2)
     return strcmp(* (char * const *) p1, * (char * const *) p2);
 }
 
+#define DEBUG_VSETS 0
 #if DEBUG_VSETS
 void debug_vsets(sr_sort_t *srt)
 {
@@ -280,6 +281,7 @@ void debug_vsets(sr_sort_t *srt)
 }
 #endif
 
+#define DEBUG_VBUF 0
 #if DEBUG_VBUF
 void debug_vbuf(sr_sort_t *srt)
 {
@@ -380,13 +382,33 @@ static int bcf_sr_sort_set(bcf_srs_t *readers, sr_sort_t *srt, const char *chr,
 
             if ( srt->str.l ) kputc(';',&srt->str);
             srt->off[srt->noff++] = srt->str.l;
-            size_t beg = srt->str.l;
+            size_t beg  = srt->str.l;
+            int end_pos = -1;
             for (ivar=1; ivar<line->n_allele; ivar++)
             {
                 if ( ivar>1 ) kputc(',',&srt->str);
                 kputs(line->d.allele[0],&srt->str);
                 kputc('>',&srt->str);
                 kputs(line->d.allele[ivar],&srt->str);
+
+                // If symbolic allele, check also the END tag in case there are multiple events,
+                // such as <DEL>s, starting at the same positions
+                if ( line->d.allele[ivar][0]=='<' )
+                {
+                    if ( end_pos==-1 )
+                    {
+                        bcf_info_t *end_info = bcf_get_info(reader->header,line,"END");
+                        if ( end_info )
+                            end_pos = (int)end_info->v1.i;  // this is only to create a unique id, we don't mind a potential int64 overflow
+                        else
+                            end_pos = 0;
+                    }
+                    if ( end_pos )
+                    {
+                        kputc('/',&srt->str);
+                        kputw(end_pos, &srt->str);
+                    }
+                }
             }
             if ( line->n_allele==1 )
             {

bgzf.c

@@ -1022,7 +1022,7 @@ int bgzf_read_block(BGZF *fp)
         if (j->hit_eof) {
             if (!fp->last_block_eof && !fp->no_eof_block) {
                 fp->no_eof_block = 1;
-                hts_log_warning("EOF marker is absent. The input is probably truncated");
+                hts_log_warning("EOF marker is absent. The input may be truncated");
             }
             fp->mt->hit_eof = 1;
         }
@@ -1124,7 +1124,7 @@ int bgzf_read_block(BGZF *fp)
         if (count == 0) { // no data read
             if (!fp->last_block_eof && !fp->no_eof_block && !fp->is_gzip) {
                 fp->no_eof_block = 1;
-                hts_log_warning("EOF marker is absent. The input is probably truncated");
+                hts_log_warning("EOF marker is absent. The input may be truncated");
             }
             fp->block_length = 0;
             return 0;
@@ -1467,7 +1467,7 @@ static void *bgzf_mt_writer(void *vp) {
 int bgzf_mt_read_block(BGZF *fp, bgzf_job *j)
 {
     uint8_t header[BLOCK_HEADER_LENGTH], *compressed_block;
-    int count, size = 0, block_length, remaining;
+    int count, block_length, remaining;
 
     // NOTE: Guaranteed to be compressed as we block multi-threading in
     // uncompressed mode.  However it may be gzip compression instead
@@ -1496,7 +1496,6 @@ int bgzf_mt_read_block(BGZF *fp, bgzf_job *j)
     if (count != sizeof(header)) // no data read
         return -1;
 
-    size = count;
     block_length = unpackInt16((uint8_t*)&header[16]) + 1; // +1 because when writing this number, we used "-1"
     if (block_length < BLOCK_HEADER_LENGTH) {
         j->errcode |= BGZF_ERR_HEADER;
@@ -1510,7 +1509,6 @@ int bgzf_mt_read_block(BGZF *fp, bgzf_job *j)
         j->errcode |= BGZF_ERR_IO;
         return -1;
     }
-    size += count;
     j->comp_len = block_length;
     j->uncomp_len = BGZF_MAX_BLOCK_SIZE;
     j->block_address = block_address;