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BACANNOT:SUMMARY, KeyError: 'tmRNA' #131

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JavariaAshraf opened this issue Feb 11, 2025 · 14 comments · May be fixed by #132
Open

BACANNOT:SUMMARY, KeyError: 'tmRNA' #131

JavariaAshraf opened this issue Feb 11, 2025 · 14 comments · May be fixed by #132

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@JavariaAshraf
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JavariaAshraf commented Feb 11, 2025

Hi fmalmeida,
i am experiencing the following error with the new version.
please help me fix the error.
below is the error:

`Command output:
(empty)

Command error:
Traceback (most recent call last):
File "/opt/conda/envs/falmeida-py/bin/falmeida-py", line 33, in
sys.exit(load_entry_point('falmeida-py==1.2.2', 'console_scripts', 'falmeida-py')())
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
File "/opt/conda/envs/falmeida-py/lib/python3.11/site-packages/falmeida_py/main.py", line 212, in main
bacannot2json(args['--input'], args['--output'], args['--print'])
File "/opt/conda/envs/falmeida-py/lib/python3.11/site-packages/falmeida_py/bacannot2json.py", line 102, in bacannot2json
general_stats( bacannot_summary )
File "/opt/conda/envs/falmeida-py/lib/python3.11/site-packages/falmeida_py/general_stats_function.py", line 43, in general_stats
bacannot_summary[sample]['general_annotation']['tmrna'] = general_results['tmRNA']
~~~~~~~~~~~~~~~^^^^^^^^^
KeyError: 'tmRNA'

Work dir:
/home/cdc-bioinfo/work/39/0a451614ccf1dfc0fe33a572b47d4d

Tip: you can replicate the issue by changing to the process work dir and entering the command bash .command.run

help needed.
thank you,
Javaria Ashraf

@fmalmeida
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Hi @JavariaAshraf ,
Can you confirm which version of the pipeline you are running?

I see this falmeida-py==1.2.2 and the latest was supposed to be this falmeida-py==1.2.4.

Thus, I want to confirm whether you are running the latest and there was a problem when I pushed the docker image.

@JavariaAshraf
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I have tried both 3.2 and 3.3.

@JavariaAshraf
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Now I am running with version v3.4.1, and got following
'WARN: The operator first is useless when applied to a value channel which returns a single value by definition '

@fmalmeida
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This warning is fine.
Can you let me know what is the outcome with v3.4.1?

@JavariaAshraf
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JavariaAshraf commented Feb 11, 2025

got following error:

Error executing process > 'BACANNOT:SOURMASH_LCA (SH33)'

  # classify
  sourmash \
      lca classify \
      --db bacannot_dbs/sourmash_db/genbank-31.lca.json.gz \
      --query SH33.fna.sig
  
  # summarize
  sourmash \
      lca summarize \
      --db bacannot_dbs/sourmash_db/genbank-31.lca.json.gz \
      --query SH33.fna.sig > SH33.fna_sourmash.summary.txt

Command exit status:
  255

Command output:
  (empty)

Command error:
  
  
  == This is sourmash version 4.8.2. ==
Caused by:
  Process `BACANNOT:SOURMASH_LCA (SH33)` terminated with an error exit status (255)

Command executed:

  # get version file
  sourmash --version > sourmash_version.txt
  
  # sketch input genome
  sourmash \
      sketch dna \
      -p scaled=1000,k=31 \
      --name-from-first \
      SH33.fna
  
  
  == Please cite Brown and Irber (2016), doi:10.21105/joss.00027. ==
  
  
  computing signatures for files: SH33.fna
  
  Computing a total of 1 signature(s) for each input.
  
  ... reading sequences from SH33.fna
  
  ...SH33.fna 800 sequences
  calculated 1 signatures for 801 sequences in SH33.fna
  
  saved 1 signature(s) to 'SH33.fna.sig'. Note: signature license is CC0.
  
  
  == This is sourmash version 4.8.2. ==
  
  == Please cite Brown and Irber (2016), doi:10.21105/joss.00027. ==
  
  
  Error! Could not find the following files. Make sure the file paths are specified correctly.
  bacannot_dbs/sourmash_db/genbank-31.lca.json.gz

Work dir:
  /home/cdc-bioinfo/work/52/94dc78825a7223d7e962705ec6f956

Tip: you can try to figure out what's wrong by changing to the process work dir and showing the script file named `.command.sh`

@fmalmeida
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fmalmeida commented Feb 11, 2025

Hi @JavariaAshraf ,
This is because the database you are using has been created with a pipeline version before v3.4.0. Thus, the expected file was not found.

The options are the following:

  • you can use the database you currently have but then skip the sourmash modules with --skip_sourmash, as this other user did for another reason here
  • or update your database, generating another using the current version of the pipeline

The option of skipping sourmash is the quickest one.

Hope that helps.

@JavariaAshraf
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JavariaAshraf commented Feb 12, 2025

I have used the option and got the following error:

'/....rror executing process > BACANNOT:GFF2GBK (SH32)

Caused by:
  Process BACANNOT:GFF2GBK (SH32) terminated with an error exit status (255)

Command executed:

  # Run emboss seqret
  seqret \
    -sequence SH32.fna \
    -feature \
    -fformat gff \
    -fopenfile SH32.gff \
    -osformat genbank \
    -osname_outseq SH32 \
    -ofdirectory_outseq gbk_file \
    -auto

Command exit status:
  255

Command output:
  (empty)

Command error:
  Warning: GFF3 tag parsing failed on '_predicted_tail_fibre_assembly_protein' in
  'ID=ALKKIJME_03776;Name=tfaE;gene=tfaE;inference=ab initio prediction:Prodigal:002006,similar to AA sequence:UniProtKB:P09153;locus_tag=ALKKIJME_03776;product=Prophage tail fiber assembly protein TfaE;Additional_database=KEGG;KO=K09810,K02003;Method=KOfamscan;Additional_database=PHAST;PHAST:Target=PHAST_16129505;PHAST:Product=Qin_prophage;_predicted_tail_fibre_assembly_protein'
  Warning: GFF3 tag parsing failed on '_essential_for_genome_maintenance' in
  'ID=ALKKIJME_02014;eC_number=2.3.1.234;Name=tsaD;db_xref=COG:COG0533;gene=tsaD;inference=ab initio prediction:Prodigal:002006,similar to AA sequence:UniProtKB:P05852;locus_tag=ALKKIJME_02014;product=tRNA N6-adenosine threonylcarbamoyltransferase;Additional_database=KEGG;KO=K07406;Method=KOfamscan;Additional_database=PHAST;PHAST:Target=PHAST_16130960;PHAST:Product=glycation_binding_protein,_predicted_protease/chaperone;_essential_for_genome_maintenance'
  Warning: GFF3 tag parsing failed on '_predicted_prophage_CPS_53_integrase' in
  'ID=ALKKIJME_03671;Name=intS_1;db_xref=COG:COG0582;gene=intS_1;inference=ab initio prediction:Prodigal:002006,similar to AA sequence:UniProtKB:P37326;locus_tag=ALKKIJME_03671;product=Prophage integrase IntS;Additional_database=PHAST;PHAST:Target=PHAST_16130281;PHAST:Product=CPS_53_(KpLE1)_prophage;_predicted_prophage_CPS_53_integrase'
  Warning: GFF3 tag parsing failed on '_predicted_tail_fibre_assembly_protein' in
  'ID=ALKKIJME_03776;Name=tfaE;gene=tfaE;inference=ab initio prediction:Prodigal:002006,similar to AA sequence:UniProtKB:P09153;locus_tag=ALKKIJME_03776;product=Prophage tail fiber assembly protein TfaE;Additional_database=KEGG;KO=K09810,K02003;Method=KOfamscan;Additional_database=PHAST;PHAST:Target=PHAST_16129505;PHAST:Product=Qin_prophage;_predicted_tail_fibre_assembly_protein'
  Warning: GFF3 tag parsing failed on '_essential_for_genome_maintenance' in
  'ID=ALKKIJME_02014;eC_number=2.3.1.234;Name=tsaD;db_xref=COG:COG0533;gene=tsaD;inference=ab initio prediction:Prodigal:002006,similar to AA sequence:UniProtKB:P05852;locus_tag=ALKKIJME_02014;product=tRNA N6-adenosine threonylcarbamoyltransferase;Additional_database=KEGG;KO=K07406;Method=KOfamscan;Additional_database=PHAST;PHAST:Target=PHAST_16130960;PHAST:Product=glycation_binding_protein,_predicted_protease/chaperone;_essential_for_genome_maintenance'
  Warning: GFF3 tag parsing failed on '_predicted_prophage_CPS_53_integrase' in
  'ID=ALKKIJME_03671;Name=intS_1;db_xref=COG:COG0582;gene=intS_1;inference=ab initio prediction:Prodigal:002006,similar to AA sequence:UniProtKB:P37326;locus_tag=ALKKIJME_03671;product=Prophage integrase IntS;Additional_database=PHAST;PHAST:Target=PHAST_16130281;PHAST:Product=CPS_53_(KpLE1)_prophage;_predicted_prophage_CPS_53_integrase'
  Warning: GFF3 tag parsing failed on '_predicted_tail_fibre_assembly_protein' in
  'ID=ALKKIJME_03776;Name=tfaE;gene=tfaE;inference=ab initio prediction:Prodigal:002006,similar to AA sequence:UniProtKB:P09153;locus_tag=ALKKIJME_03776;product=Prophage tail fiber assembly protein TfaE;Additional_database=KEGG;KO=K09810,K02003;Method=KOfamscan;Additional_database=PHAST;PHAST:Target=PHAST_16129505;PHAST:Product=Qin_prophage;_predicted_tail_fibre_assembly_protein'
  Warning: GFF3 tag parsing failed on '_essential_for_genome_maintenance' in
  'ID=ALKKIJME_02014;eC_number=2.3.1.234;Name=tsaD;db_xref=COG:COG0533;gene=tsaD;inference=ab initio prediction:Prodigal:002006,similar to AA sequence:UniProtKB:P05852;locus_tag=ALKKIJME_02014;product=tRNA N6-adenosine threonylcarbamoyltransferase;Additional_database=KEGG;KO=K07406;Method=KOfamscan;Additional_database=PHAST;PHAST:Target=PHAST_16130960;PHAST:Product=glycation_binding_protein,_predicted_protease/chaperone;_essential_for_genome_maintenance'
  Warning: GFF3 tag parsing failed on '_predicted_prophage_CPS_53_integrase' in
  'ID=ALKKIJME_03671;Name=intS_1;db_xref=COG:COG0582;gene=intS_1;inference=ab initio prediction:Prodigal:002006,similar to AA sequence:UniProtKB:P37326;locus_tag=ALKKIJME_03671;product=Prophage integrase IntS;Additional_database=PHAST;PHAST:Target=PHAST_16130281;PHAST:Product=CPS_53_(KpLE1)_prophage;_predicted_prophage_CPS_53_integrase'
  Warning: GFF3 tag parsing failed on '_predicted_tail_fibre_assembly_protein' in
  'ID=ALKKIJME_03776;Name=tfaE;gene=tfaE;inference=ab initio prediction:Prodigal:002006,similar to AA sequence:UniProtKB:P09153;locus_tag=ALKKIJME_03776;product=Prophage tail fiber assembly protein TfaE;Additional_database=KEGG;KO=K09810,K02003;Method=KOfamscan;Additional_database=PHAST;PHAST:Target=PHAST_16129505;PHAST:Product=Qin_prophage;_predicted_tail_fibre_assembly_protein'
  Warning: GFF3 tag parsing failed on '_essential_for_genome_maintenance' in
  'ID=ALKKIJME_02014;eC_number=2.3.1.234;Name=tsaD;db_xref=COG:COG0533;gene=tsaD;inference=ab initio prediction:Prodigal:002006,similar to AA sequence:UniProtKB:P05852;locus_tag=ALKKIJME_02014;product=tRNA N6-adenosine threonylcarbamoyltransferase;Additional_database=KEGG;KO=K07406;Method=KOfamscan;Additional_database=PHAST;PHAST:Target=PHAST_16130960;PHAST:Product=glycation_binding_protein,_predicted_protease/chaperone;_essential_for_genome_maintenance'
  Warning: GFF3 tag parsing failed on '_predicted_prophage_CPS_53_integrase' in
  'ID=ALKKIJME_03671;Name=intS_1;db_xref=COG:COG0582;gene=intS_1;inference=ab initio prediction:Prodigal:002006,similar to AA sequence:UniProtKB:P37326;locus_tag=ALKKIJME_03671;product=Prophage integrase IntS;Additional_database=PHAST;PHAST:Target=PHAST_16130281;PHAST:Product=CPS_53_(KpLE1)_prophage;_predicted_prophage_CPS_53_integrase'
  Warning: GFF3 tag parsing failed on '_predicted_tail_fibre_assembly_protein' in
  'ID=ALKKIJME_03776;Name=tfaE;gene=tfaE;inference=ab initio prediction:Prodigal:002006,similar to AA sequence:UniProtKB:P09153;locus_tag=ALKKIJME_03776;product=Prophage tail fiber assembly protein TfaE;Additional_database=KEGG;KO=K09810,K02003;Method=KOfamscan;Additional_database=PHAST;PHAST:Target=PHAST_16129505;PHAST:Product=Qin_prophage;_predicted_tail_fibre_assembly_protein'
  Warning: GFF3 tag parsing failed on '_essential_for_genome_maintenance' in
  'ID=ALKKIJME_02014;eC_number=2.3.1.234;Name=tsaD;db_xref=COG:COG0533;gene=tsaD;inference=ab initio prediction:Prodigal:002006,similar to AA sequence:UniProtKB:P05852;locus_tag=ALKKIJME_02014;product=tRNA N6-adenosine threonylcarbamoyltransferase;Additional_database=KEGG;KO=K07406;Method=KOfamscan;Additional_database=PHAST;PHAST:Target=PHAST_16130960;PHAST:Product=glycation_binding_protein,_predicted_protease/chaperone;_essential_for_genome_maintenance'
  Warning: GFF3 tag parsing failed on '_predicted_prophage_CPS_53_integrase' in
  'ID=ALKKIJME_03671;Name=intS_1;db_xref=COG:COG0582;gene=intS_1;inference=ab initio prediction:Prodigal:002006,similar to AA sequence:UniProtKB:P37326;locus_tag=ALKKIJME_03671;product=Prophage integrase IntS;Additional_database=PHAST;PHAST:Target=PHAST_16130281;PHAST:Product=CPS_53_(KpLE1)_prophage;_predicted_prophage_CPS_53_integrase'
  Warning: GFF3 tag parsing failed on '_predicted_tail_fibre_assembly_protein' in
  'ID=ALKKIJME_03776;Name=tfaE;gene=tfaE;inference=ab initio prediction:Prodigal:002006,similar to AA sequence:UniProtKB:P09153;locus_tag=ALKKIJME_03776;product=Prophage tail fiber assembly protein TfaE;Additional_database=KEGG;KO=K09810,K02003;Method=KOfamscan;Additional_database=PHAST;PHAST:Target=PHAST_16129505;PHAST:Product=Qin_prophage;_predicted_tail_fibre_assembly_protein'
  Warning: GFF3 tag parsing failed on '_essential_for_genome_maintenance' in
  'ID=ALKKIJME_02014;eC_number=2.3.1.234;Name=tsaD;db_xref=COG:COG0533;gene=tsaD;inference=ab initio prediction:Prodigal:002006,similar to AA sequence:UniProtKB:P05852;locus_tag=ALKKIJME_02014;product=tRNA N6-adenosine threonylcarbamoyltransferase;Additional_database=KEGG;KO=K07406;Method=KOfamscan;Additional_database=PHAST;PHAST:Target=PHAST_16130960;PHAST:Product=glycation_binding_protein,_predicted_protease/chaperone;_essential_for_genome_maintenance'
  Warning: GFF3 tag parsing failed on '_predicted_prophage_CPS_53_integrase' in
  'ID=ALKKIJME_03671;Name=intS_1;db_xref=COG:COG0582;gene=intS_1;inference=ab initio prediction:Prodigal:002006,similar to AA sequence:UniProtKB:P37326;locus_tag=ALKKIJME_03671;product=Prophage integrase IntS;Additional_database=PHAST;PHAST:Target=PHAST_16130281;PHAST:Product=CPS_53_(KpLE1)_prophage;_predicted_prophage_CPS_53_integrase'
  Warning: GFF3 tag parsing failed on '_predicted_tail_fibre_assembly_protein' in
  'ID=ALKKIJME_03776;Name=tfaE;gene=tfaE;inference=ab initio prediction:Prodigal:002006,similar to AA sequence:UniProtKB:P09153;locus_tag=ALKKIJME_03776;product=Prophage tail fiber assembly protein TfaE;Additional_database=KEGG;KO=K09810,K02003;Method=KOfamscan;Additional_database=PHAST;PHAST:Target=PHAST_16129505;PHAST:Product=Qin_prophage;_predicted_tail_fibre_assembly_protein'
  Warning: GFF3 tag parsing failed on '_essential_for_genome_maintenance' in
  'ID=ALKKIJME_02014;eC_number=2.3.1.234;Name=tsaD;db_xref=COG:COG0533;gene=tsaD;inference=ab initio prediction:Prodigal:002006,similar to AA sequence:UniProtKB:P05852;locus_tag=ALKKIJME_02014;product=tRNA N6-adenosine threonylcarbamoyltransferase;Additional_database=KEGG;KO=K07406;Method=KOfamscan;Additional_database=PHAST;PHAST:Target=PHAST_16130960;PHAST:Product=glycation_binding_protein,_predicted_protease/chaperone;_essential_for_genome_maintenance'
  Warning: GFF3 tag parsing failed on '_predicted_prophage_CPS_53_integrase' in
  'ID=ALKKIJME_03671;Name=intS_1;db_xref=COG:COG0582;gene=intS_1;inference=ab initio prediction:Prodigal:002006,similar to AA sequence:UniProtKB:P37326;locus_tag=ALKKIJME_03671;product=Prophage integrase IntS;Additional_database=PHAST;PHAST:Target=PHAST_16130281;PHAST:Product=CPS_53_(KpLE1)_prophage;_predicted_prophage_CPS_53_integrase'
  Warning: GFF3 tag parsing failed on '_predicted_tail_fibre_assembly_protein' in
  'ID=ALKKIJME_03776;Name=tfaE;gene=tfaE;inference=ab initio prediction:Prodigal:002006,similar to AA sequence:UniProtKB:P09153;locus_tag=ALKKIJME_03776;product=Prophage tail fiber assembly protein TfaE;Additional_database=KEGG;KO=K09810,K02003;Method=KOfamscan;Additional_database=PHAST;PHAST:Target=PHAST_16129505;PHAST:Product=Qin_prophage;_predicted_tail_fibre_assembly_protein'
  Warning: GFF3 tag parsing failed on '_essential_for_genome_maintenance' in
  'ID=ALKKIJME_02014;eC_number=2.3.1.234;Name=tsaD;db_xref=COG:COG0533;gene=tsaD;inference=ab initio prediction:Prodigal:002006,similar to AA sequence:UniProtKB:P05852;locus_tag=ALKKIJME_02014;product=tRNA N6-adenosine threonylcarbamoyltransferase;Additional_database=KEGG;KO=K07406;Method=KOfamscan;Additional_database=PHAST;PHAST:Target=PHAST_16130960;PHAST:Product=glycation_binding_protein,_predicted_protease/chaperone;_essential_for_genome_maintenance'
  Warning: GFF3 tag parsing failed on '_predicted_prophage_CPS_53_integrase' in
  'ID=ALKKIJME_03671;Name=intS_1;db_xref=COG:COG0582;gene=intS_1;inference=ab initio prediction:Prodigal:002006,similar to AA sequence:UniProtKB:P37326;locus_tag=ALKKIJME_03671;product=Prophage integrase IntS;Additional_database=PHAST;PHAST:Target=PHAST_16130281;PHAST:Product=CPS_53_(KpLE1)_prophage;_predicted_prophage_CPS_53_integrase'
  Warning: GFF3 tag parsing failed on '_predicted_tail_fibre_assembly_protein' in
  'ID=ALKKIJME_03776;Name=tfaE;gene=tfaE;inference=ab initio prediction:Prodigal:002006,similar to AA sequence:UniProtKB:P09153;locus_tag=ALKKIJME_03776;product=Prophage tail fiber assembly protein TfaE;Additional_database=KEGG;KO=K09810,K02003;Method=KOfamscan;Additional_da'"

;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;

please have a look.
Thank you.

@fmalmeida
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fmalmeida commented Feb 12, 2025

Hi @JavariaAshraf ,

checking the error PHAST:Product=glycation_binding_protein,_predicted_protease/chaperone;_essential_for_genome_maintenance it is clear to me that the problem comes from the PHAST database file which have some entries in their fasta containing the ; character.

We can see here that indeed this seems to be the case:

grep ';' bacannot_dbs_2024_jul_05/phast_db/sequences | head -n 2
>PHAST~~~Qin_prophage;_predicted_tail_fibre_assembly_protein~~~NP_416064.1~~~PHAST_16129505~~~PROPHAGE_Escher_MG1655-gi|16129505|ref|NP_416064.1| Qin prophage; predicted tail fibre assembly protein [Escherichia coli str. K-12 substr. MG1655]
>PHAST~~~Qin_prophage;_predicted_side_tail_fibre_assembly_protein~~~NP_416065.1~~~PHAST_16129506~~~PROPHAGE_Escher_MG1655-gi|16129506|ref|NP_416065.1| Qin prophage; predicted side tail fibre assembly protein [Escherichia coli str. K-12 substr. MG1655]

This is an error that I only saw now because I never executed with this species. Thanks to you to have noticed such scenario.

The proper fix

The correct fix for the pipeline would be the following:

  1. I add a sed operation when downloading and preparing the PHAST database to remove this character and avoid breaking the pipeline
  2. Then I make a pipeline hotfix for you to test before
  3. Once tested, I can release. And then, you would have to update your database directory (downloading again with the latest pipeline version).

This round would take a while and we probably can finish next week.

The quick fix

If you really need to have some results the soonest possible, I would suggest you run the same command line, but add the following parameter --skip_prophage_search, so that these prophage modules are not executed and you can have the other results.

Let me know which one you are more inclined to. Anyways, I will start (in this branch) with the ""the proper fix"" solution and will ping here in this thread once I would have something for you to test.

@fmalmeida
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Also, is this a private genome or a public genome? Was just wondering if I could test with the same sequence while implementing.

@fmalmeida
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fmalmeida commented Feb 12, 2025

Hi @JavariaAshraf ,
I have some update for the ""the proper fix"" solution. I have something to test in its specific branch (131-bacannotsummary-keyerror-tmrna).

Assuming you have the bacannot database in the following path: /path-to/bacannot_dbs can you test out the following?

  1. Delete the PHAST database in the database
    • rm -rf /path-to/bacannot_dbs/phast_db
  2. Download the PHAST database again with the implementation branch
    • nextflow run fmalmeida/bacannot -r 131-bacannotsummary-keyerror-tmrna -latest -profile docker --get_dbs --output /path-to/bacannot_dbs/phast_db
    • The pipeline should be smart enough to only download the missing databases.
  3. Then run the pipeline again, using the same command line you had but with the implementation branch
    • nextflow run fmalmeida/bacannot -r 131-bacannotsummary-keyerror-tmrna -latest -profile docker --bacannot_db --output /path-to/bacannot_dbs -resume <all the other parameters you had>

To assess whether the code added fixes the bug or not.

If it does, then I can go for a release. If it does not, or something else appears, I have to check the code again.

@fmalmeida fmalmeida linked a pull request Feb 13, 2025 that will close this issue
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@fmalmeida fmalmeida linked a pull request Feb 13, 2025 that will close this issue
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@JavariaAshraf
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JavariaAshraf commented Feb 14, 2025

Hi fmalmeida,
There is another error: Please see below

WARN:Cannot read project manifest -- Cause: Remote resource not found: https://api.github.com/repos/nextflow/bacannot/contents/nextflow.config?ref=131-bacannotsummary-keyerror-tmrna
Remote resource not found: https://api.github.com/repos/nextflow/bacannot/contents/main.nf?ref=131-bacannotsummary-keyerror-tmrna

It occurred while I was following your defined steps in the proper fix.

@fmalmeida
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Hi @JavariaAshraf ,

Was just a misspelling in the command line. I did not have the proper name fmalmeida/bacannot in them.

I have just updated the comment with the correct spelling. Can you try again?

@JavariaAshraf
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getting the same error still.

@fmalmeida
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fmalmeida commented Feb 14, 2025

What command exactly you executed? You mean the last error or the first?

It should be fmalmeida/bacannot and not nextflow/bacannot

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