diff --git a/op-acceptance-tests/acceptance-tests.yaml b/op-acceptance-tests/acceptance-tests.yaml
index 1a606f1872f9d..a0d677689bbdc 100644
--- a/op-acceptance-tests/acceptance-tests.yaml
+++ b/op-acceptance-tests/acceptance-tests.yaml
@@ -78,6 +78,14 @@ gates:
     tests:
       - package: github.com/ethereum-optimism/optimism/op-acceptance-tests/tests/flashblocks
         timeout: 5m
+  
+  - id: flashblocks-with-isthmus
+    inherits:
+      - isthmus
+    description: "Flashblocks network tests with Isthmus."
+    tests:
+      - package: github.com/ethereum-optimism/optimism/op-acceptance-tests/tests/flashblocks
+        timeout: 5m
 
   - id: sync-test-op-node
     description: "Sync tests for op-node with external networks via the op-sync-tester - tests run daily."