diff --git a/op-acceptance-tests/acceptance-tests.yaml b/op-acceptance-tests/acceptance-tests.yaml
index 5e89172002bc0..4d732ebad16dc 100644
--- a/op-acceptance-tests/acceptance-tests.yaml
+++ b/op-acceptance-tests/acceptance-tests.yaml
@@ -31,6 +31,15 @@ gates:
       - package: github.com/ethereum-optimism/optimism/op-acceptance-tests/tests/isthmus/operator_fee
         timeout: 6h
 
+  - id: pre-interop
+    inherits:
+      - base
+    description: "Pre-interop network tests."
+    tests:
+      - name: TestInteropReadiness
+        package: github.com/ethereum-optimism/optimism/op-acceptance-tests/tests/isthmus/preinterop
+        timeout: 20m
+
   - id: interop
     inherits:
       - base
diff --git a/op-acceptance-tests/tests/isthmus/preinterop/interop_readiness_test.go b/op-acceptance-tests/tests/isthmus/preinterop/interop_readiness_test.go
new file mode 100644
index 0000000000000..17d0bdcd1efcf
--- /dev/null
+++ b/op-acceptance-tests/tests/isthmus/preinterop/interop_readiness_test.go
@@ -0,0 +1,170 @@
+package base
+
+import (
+	"bytes"
+	"context"
+	"fmt"
+	"testing"
+
+	"github.com/ethereum-optimism/optimism/devnet-sdk/system"
+	"github.com/ethereum-optimism/optimism/devnet-sdk/testing/systest"
+	"github.com/ethereum-optimism/optimism/op-e2e/bindings"
+	"github.com/ethereum-optimism/optimism/op-service/sources/batching"
+	"github.com/ethereum-optimism/optimism/op-service/sources/batching/rpcblock"
+	"github.com/ethereum-optimism/optimism/op-service/testlog"
+	"github.com/ethereum/go-ethereum/accounts/abi"
+	"github.com/ethereum/go-ethereum/common"
+	"github.com/ethereum/go-ethereum/ethclient"
+	"github.com/ethereum/go-ethereum/log"
+	"github.com/stretchr/testify/require"
+)
+
+var portalABIString = `
+[
+	{
+		"inputs": [],
+		"name": "proxyAdminOwner",
+		"outputs": [{"name": "", "type": "address"}],
+		"stateMutability": "view",
+		"type": "function"
+	},
+	{
+		"inputs": [],
+		"name": "superchainConfig",
+		"outputs": [{"name": "", "type": "address"}],
+		"stateMutability": "view",
+		"type": "function"
+	},
+	{
+    	"inputs": [],
+		"name": "respectedGameType",
+		"outputs": [
+		{
+			"name": "",
+			"type": "uint32"
+		}
+		],
+		"stateMutability": "view",
+		"type": "function"
+	}
+]
+`
+
+var portalABI *abi.ABI
+
+func init() {
+	if parsed, err := abi.JSON(bytes.NewReader([]byte(portalABIString))); err != nil {
+		panic(fmt.Sprintf("failed to parse portal abi: %s", err))
+	} else {
+		portalABI = &parsed
+	}
+}
+
+func TestInteropReadiness(t *testing.T) {
+	systest.SystemTest(t, interopReadinessTestSenario())
+}
+
+func interopReadinessTestSenario() systest.SystemTestFunc {
+	return func(t systest.T, sys system.System) {
+		logger := testlog.Logger(t, log.LevelInfo)
+		logger.Info("Started test")
+
+		l1Client, err := sys.L1().Nodes()[0].GethClient()
+		require.NoError(t, err)
+		l1Caller := batching.NewMultiCaller(l1Client.Client(), batching.DefaultBatchSize)
+
+		checkAbsolutePrestate(t, sys, l1Client)
+		checkL1PAO(t, sys, l1Caller)
+		checkSuperchainConfig(t, sys, l1Caller)
+		checkPermissionless(t, sys, l1Caller)
+	}
+}
+
+func checkAbsolutePrestate(t systest.T, sys system.System, l1Client *ethclient.Client) {
+	var prestate *[32]byte
+	for _, chain := range sys.L2s() {
+		p := getPrestate(t, l1Client, chain)
+		if prestate == nil {
+			prestate = &p
+		} else {
+			require.Equal(t, *prestate, p)
+		}
+	}
+	require.NotNil(t, prestate)
+}
+
+func checkL1PAO(t systest.T, sys system.System, l1Caller *batching.MultiCaller) {
+	var l1PAO common.Address
+	for _, chain := range sys.L2s() {
+		owner := getL1PAO(t, l1Caller, chain)
+		if l1PAO == (common.Address{}) {
+			l1PAO = owner
+		} else {
+			require.Equal(t, l1PAO, owner)
+		}
+	}
+	require.NotNil(t, l1PAO)
+}
+
+func checkSuperchainConfig(t systest.T, sys system.System, l1Caller *batching.MultiCaller) {
+	var superchainConfig common.Address
+	for _, chain := range sys.L2s() {
+		address := getSuperchainConfigFromPortal(t, l1Caller, chain)
+		if superchainConfig == (common.Address{}) {
+			superchainConfig = address
+		} else {
+			require.Equal(t, superchainConfig, address)
+		}
+	}
+	require.NotNil(t, superchainConfig)
+}
+
+func checkPermissionless(t systest.T, sys system.System, l1Caller *batching.MultiCaller) {
+	for _, chain := range sys.L2s() {
+		gameType := getRespectedGameType(t, l1Caller, chain)
+		require.Equal(t, uint32(0), gameType, "chain is not permissionless")
+	}
+}
+
+func getL1PAO(t systest.T, l1Caller *batching.MultiCaller, l2Chain system.L2Chain) common.Address {
+	portalAddress, ok := l2Chain.L1Addresses()["OptimismPortalProxy"]
+	require.True(t, ok, "OptimismPortalProxy not found")
+	contract := batching.NewBoundContract(portalABI, portalAddress)
+	results, err := l1Caller.SingleCall(context.Background(), rpcblock.Latest, contract.Call("proxyAdminOwner"))
+	require.NoError(t, err)
+	return results.GetAddress(0)
+}
+
+func getSuperchainConfigFromPortal(t systest.T, l1Caller *batching.MultiCaller, l2Chain system.L2Chain) common.Address {
+	portalAddress, ok := l2Chain.L1Addresses()["OptimismPortalProxy"]
+	require.True(t, ok, "OptimismPortalProxy not found")
+	contract := batching.NewBoundContract(portalABI, portalAddress)
+	results, err := l1Caller.SingleCall(context.Background(), rpcblock.Latest, contract.Call("superchainConfig"))
+	require.NoError(t, err)
+	return results.GetAddress(0)
+}
+
+func getPrestate(t systest.T, l1Client *ethclient.Client, l2Chain system.L2Chain) [32]byte {
+	dgf, ok := l2Chain.L1Addresses()["DisputeGameFactoryProxy"]
+	require.True(t, ok, "DisputeGameFactoryProxy not found")
+	dgfContract, err := bindings.NewDisputeGameFactory(dgf, l1Client)
+	require.NoError(t, err)
+
+	gameImpl, err := dgfContract.GameImpls(nil, 0)
+	require.NoError(t, err)
+	fdgContract, err := bindings.NewFaultDisputeGame(gameImpl, l1Client)
+	require.NoError(t, err)
+
+	prestate, err := fdgContract.AbsolutePrestate(nil)
+	require.NoError(t, err)
+	return prestate
+}
+
+func getRespectedGameType(t systest.T, l1Caller *batching.MultiCaller, l2Chain system.L2Chain) uint32 {
+	portalAddress, ok := l2Chain.L1Addresses()["OptimismPortalProxy"]
+	require.True(t, ok, "OptimismPortalProxy not found")
+	contract := batching.NewBoundContract(portalABI, portalAddress)
+	results, err := l1Caller.SingleCall(context.Background(), rpcblock.Latest, contract.Call("respectedGameType"))
+	require.NoError(t, err)
+	return results.GetUint32(0)
+}