diff --git a/internal/gatewayapi/route.go b/internal/gatewayapi/route.go
index 2afdf86dc6..e960370398 100644
--- a/internal/gatewayapi/route.go
+++ b/internal/gatewayapi/route.go
@@ -1034,7 +1034,11 @@ func (t *Translator) processUDPRouteParentRefs(udpRoute *UDPRouteContext, resour
 				)
 				continue
 			}
-			destSettings = append(destSettings, ds)
+
+			// Skip nil destination settings
+			if ds != nil {
+				destSettings = append(destSettings, ds)
+			}
 		}
 
 		// If no negative condition has been set for ResolvedRefs, set "ResolvedRefs=True"
@@ -1176,7 +1180,10 @@ func (t *Translator) processTCPRouteParentRefs(tcpRoute *TCPRouteContext, resour
 				)
 				continue
 			}
-			destSettings = append(destSettings, ds)
+			// Skip nil destination settings
+			if ds != nil {
+				destSettings = append(destSettings, ds)
+			}
 		}
 
 		// If no negative condition has been set for ResolvedRefs, set "ResolvedRefs=True"
diff --git a/internal/gatewayapi/testdata/tcproute-rule-with-multiple-backends-and-zero-weights.in.yaml b/internal/gatewayapi/testdata/tcproute-rule-with-multiple-backends-and-zero-weights.in.yaml
new file mode 100644
index 0000000000..af361628fa
--- /dev/null
+++ b/internal/gatewayapi/testdata/tcproute-rule-with-multiple-backends-and-zero-weights.in.yaml
@@ -0,0 +1,39 @@
+gateways:
+  - apiVersion: gateway.networking.k8s.io/v1
+    kind: Gateway
+    metadata:
+      namespace: envoy-gateway
+      name: gateway-1
+    spec:
+      gatewayClassName: envoy-gateway-class
+      listeners:
+        - name: tcp
+          protocol: TCP
+          port: 90
+          allowedRoutes:
+            namespaces:
+              from: All
+tcpRoutes:
+  - apiVersion: gateway.networking.k8s.io/v1alpha2
+    kind: TCPRoute
+    metadata:
+      namespace: default
+      name: tcproute-1
+    spec:
+      parentRefs:
+        - namespace: envoy-gateway
+          name: gateway-1
+      rules:
+        - backendRefs:
+            - name: service-1
+              port: 8080
+              weight: 1
+            - name: service-2
+              port: 8080
+              weight: 2
+            - name: service-3
+              port: 8080
+              weight: 3
+            - name: service-4
+              port: 8080
+              weight: 0
diff --git a/internal/gatewayapi/testdata/tcproute-rule-with-multiple-backends-and-zero-weights.out.yaml b/internal/gatewayapi/testdata/tcproute-rule-with-multiple-backends-and-zero-weights.out.yaml
new file mode 100644
index 0000000000..bd9d641561
--- /dev/null
+++ b/internal/gatewayapi/testdata/tcproute-rule-with-multiple-backends-and-zero-weights.out.yaml
@@ -0,0 +1,134 @@
+gateways:
+- apiVersion: gateway.networking.k8s.io/v1
+  kind: Gateway
+  metadata:
+    creationTimestamp: null
+    name: gateway-1
+    namespace: envoy-gateway
+  spec:
+    gatewayClassName: envoy-gateway-class
+    listeners:
+    - allowedRoutes:
+        namespaces:
+          from: All
+      name: tcp
+      port: 90
+      protocol: TCP
+  status:
+    listeners:
+    - attachedRoutes: 1
+      conditions:
+      - lastTransitionTime: null
+        message: Sending translated listener configuration to the data plane
+        reason: Programmed
+        status: "True"
+        type: Programmed
+      - lastTransitionTime: null
+        message: Listener has been successfully translated
+        reason: Accepted
+        status: "True"
+        type: Accepted
+      - lastTransitionTime: null
+        message: Listener references have been resolved
+        reason: ResolvedRefs
+        status: "True"
+        type: ResolvedRefs
+      name: tcp
+      supportedKinds:
+      - group: gateway.networking.k8s.io
+        kind: TCPRoute
+infraIR:
+  envoy-gateway/gateway-1:
+    proxy:
+      listeners:
+      - address: null
+        name: envoy-gateway/gateway-1/tcp
+        ports:
+        - containerPort: 10090
+          name: tcp-90
+          protocol: TCP
+          servicePort: 90
+      metadata:
+        labels:
+          gateway.envoyproxy.io/owning-gateway-name: gateway-1
+          gateway.envoyproxy.io/owning-gateway-namespace: envoy-gateway
+      name: envoy-gateway/gateway-1
+tcpRoutes:
+- apiVersion: gateway.networking.k8s.io/v1alpha2
+  kind: TCPRoute
+  metadata:
+    creationTimestamp: null
+    name: tcproute-1
+    namespace: default
+  spec:
+    parentRefs:
+    - name: gateway-1
+      namespace: envoy-gateway
+    rules:
+    - backendRefs:
+      - name: service-1
+        port: 8080
+        weight: 1
+      - name: service-2
+        port: 8080
+        weight: 2
+      - name: service-3
+        port: 8080
+        weight: 3
+      - name: service-4
+        port: 8080
+        weight: 0
+  status:
+    parents:
+    - conditions:
+      - lastTransitionTime: null
+        message: Route is accepted
+        reason: Accepted
+        status: "True"
+        type: Accepted
+      - lastTransitionTime: null
+        message: Resolved all the Object references for the Route
+        reason: ResolvedRefs
+        status: "True"
+        type: ResolvedRefs
+      controllerName: gateway.envoyproxy.io/gatewayclass-controller
+      parentRef:
+        name: gateway-1
+        namespace: envoy-gateway
+xdsIR:
+  envoy-gateway/gateway-1:
+    accessLog:
+      text:
+      - path: /dev/stdout
+    readyListener:
+      address: 0.0.0.0
+      ipFamily: IPv4
+      path: /ready
+      port: 19003
+    tcp:
+    - address: 0.0.0.0
+      name: envoy-gateway/gateway-1/tcp
+      port: 10090
+      routes:
+      - destination:
+          name: tcproute/default/tcproute-1/rule/-1
+          settings:
+          - addressType: IP
+            endpoints:
+            - host: 7.7.7.7
+              port: 8080
+            protocol: TCP
+            weight: 1
+          - addressType: IP
+            endpoints:
+            - host: 7.7.7.7
+              port: 8080
+            protocol: TCP
+            weight: 2
+          - addressType: IP
+            endpoints:
+            - host: 7.7.7.7
+              port: 8080
+            protocol: TCP
+            weight: 3
+        name: tcproute/default/tcproute-1
diff --git a/internal/gatewayapi/testdata/udproute-rule-with-multiple-backends-and-zero-weights.in.yaml b/internal/gatewayapi/testdata/udproute-rule-with-multiple-backends-and-zero-weights.in.yaml
new file mode 100644
index 0000000000..53851284ee
--- /dev/null
+++ b/internal/gatewayapi/testdata/udproute-rule-with-multiple-backends-and-zero-weights.in.yaml
@@ -0,0 +1,39 @@
+gateways:
+  - apiVersion: gateway.networking.k8s.io/v1
+    kind: Gateway
+    metadata:
+      namespace: envoy-gateway
+      name: gateway-1
+    spec:
+      gatewayClassName: envoy-gateway-class
+      listeners:
+        - name: udp
+          protocol: UDP
+          port: 90
+          allowedRoutes:
+            namespaces:
+              from: All
+udpRoutes:
+  - apiVersion: gateway.networking.k8s.io/v1alpha2
+    kind: UDPRoute
+    metadata:
+      namespace: default
+      name: udproute-1
+    spec:
+      parentRefs:
+        - namespace: envoy-gateway
+          name: gateway-1
+      rules:
+        - backendRefs:
+            - name: service-1
+              port: 8162
+              weight: 1
+            - name: service-2
+              port: 8162
+              weight: 2
+            - name: service-3
+              port: 8162
+              weight: 3
+            - name: service-4
+              port: 8162
+              weight: 0
diff --git a/internal/gatewayapi/testdata/udproute-rule-with-multiple-backends-and-zero-weights.out.yaml b/internal/gatewayapi/testdata/udproute-rule-with-multiple-backends-and-zero-weights.out.yaml
new file mode 100644
index 0000000000..e3d2bbac4d
--- /dev/null
+++ b/internal/gatewayapi/testdata/udproute-rule-with-multiple-backends-and-zero-weights.out.yaml
@@ -0,0 +1,134 @@
+gateways:
+- apiVersion: gateway.networking.k8s.io/v1
+  kind: Gateway
+  metadata:
+    creationTimestamp: null
+    name: gateway-1
+    namespace: envoy-gateway
+  spec:
+    gatewayClassName: envoy-gateway-class
+    listeners:
+    - allowedRoutes:
+        namespaces:
+          from: All
+      name: udp
+      port: 90
+      protocol: UDP
+  status:
+    listeners:
+    - attachedRoutes: 1
+      conditions:
+      - lastTransitionTime: null
+        message: Sending translated listener configuration to the data plane
+        reason: Programmed
+        status: "True"
+        type: Programmed
+      - lastTransitionTime: null
+        message: Listener has been successfully translated
+        reason: Accepted
+        status: "True"
+        type: Accepted
+      - lastTransitionTime: null
+        message: Listener references have been resolved
+        reason: ResolvedRefs
+        status: "True"
+        type: ResolvedRefs
+      name: udp
+      supportedKinds:
+      - group: gateway.networking.k8s.io
+        kind: UDPRoute
+infraIR:
+  envoy-gateway/gateway-1:
+    proxy:
+      listeners:
+      - address: null
+        name: envoy-gateway/gateway-1/udp
+        ports:
+        - containerPort: 10090
+          name: udp-90
+          protocol: UDP
+          servicePort: 90
+      metadata:
+        labels:
+          gateway.envoyproxy.io/owning-gateway-name: gateway-1
+          gateway.envoyproxy.io/owning-gateway-namespace: envoy-gateway
+      name: envoy-gateway/gateway-1
+udpRoutes:
+- apiVersion: gateway.networking.k8s.io/v1alpha2
+  kind: UDPRoute
+  metadata:
+    creationTimestamp: null
+    name: udproute-1
+    namespace: default
+  spec:
+    parentRefs:
+    - name: gateway-1
+      namespace: envoy-gateway
+    rules:
+    - backendRefs:
+      - name: service-1
+        port: 8162
+        weight: 1
+      - name: service-2
+        port: 8162
+        weight: 2
+      - name: service-3
+        port: 8162
+        weight: 3
+      - name: service-4
+        port: 8162
+        weight: 0
+  status:
+    parents:
+    - conditions:
+      - lastTransitionTime: null
+        message: Route is accepted
+        reason: Accepted
+        status: "True"
+        type: Accepted
+      - lastTransitionTime: null
+        message: Resolved all the Object references for the Route
+        reason: ResolvedRefs
+        status: "True"
+        type: ResolvedRefs
+      controllerName: gateway.envoyproxy.io/gatewayclass-controller
+      parentRef:
+        name: gateway-1
+        namespace: envoy-gateway
+xdsIR:
+  envoy-gateway/gateway-1:
+    accessLog:
+      text:
+      - path: /dev/stdout
+    readyListener:
+      address: 0.0.0.0
+      ipFamily: IPv4
+      path: /ready
+      port: 19003
+    udp:
+    - address: 0.0.0.0
+      name: envoy-gateway/gateway-1/udp
+      port: 10090
+      route:
+        destination:
+          name: udproute/default/udproute-1/rule/-1
+          settings:
+          - addressType: IP
+            endpoints:
+            - host: 7.7.7.7
+              port: 8162
+            protocol: UDP
+            weight: 1
+          - addressType: IP
+            endpoints:
+            - host: 7.7.7.7
+              port: 8162
+            protocol: UDP
+            weight: 2
+          - addressType: IP
+            endpoints:
+            - host: 7.7.7.7
+              port: 8162
+            protocol: UDP
+            weight: 3
+        name: udproute/default/udproute-1