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Paper "Description of new species of Mycobacterium terrae complex isolated from sewage at the São Paulo Zoological Park Foundation in Brazil"

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emilyncosta/UNIFESP_NTM_newspecies-nf

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Cite with Zenodo

Nextflow run with conda run with docker run with singularity Launch on Nextflow Tower

Introduction

UNIFESP_ntm_newspecies_nf is a bioinformatics pipeline used for the paper entitled Description of new species of Mycobacterium terrae complex isolated from sewage at the São Paulo Zoological Park Foundation in Brazil

IDs for the submitted FASTQ samples

Sample ID ATCC ID JCM ID Nomenclature Nomenclature alias (Gupta et a. 2018) GenBanK 16S rRNA gene Bioproject Biosample SRA GenBank Draft Genome PGAP Annotation
MYC017 TSD-296T 35364T Mycobacterium vasticus Mycolicibacter vasticus MK890459.1 PRJNA755977 SAMN20959233 SRR27405758 CP084028 JAYJJQ000000000
MYC098 TSD-297T 35365T Mycobacterium crassicus Mycolicibacter crassicus MK890478.1 PRJNA757362 SAMN20959234 SRR27405950 CP084029 JAYJJR000000000
MYC101 TSD-298T 35366T Mycobacterium zoologicum Mycolicibacter zoologicum MK890479.1 PRJNA757364 SAMN20959235 SRR27405954 CP084030 JAYJJS000000000
MYC123 BAA3216 35367 Mycobacterium zoologicum Mycolicibacter zoologicum MK890481.1 PRJNA743883 SAMN20062777 SRR27406169 CP083985 JAYJJT000000000
MYC340 TSD-299T 35368T Mycobacterium nativiensis Mycolicibacter nativiensis MK890521.1 PRJNA743885 SAMN20062778 SRR27406220 CP083986 JAYJJU000000000

Usage

Note If you are new to Nextflow and nf-core, please refer to this page on how to set-up Nextflow. Make sure to test your setup with -profile test before running the workflow on actual data.

Now, you can run the pipeline using:

nextflow run emilyncosta/UNIFESP_ntm_newspecies_nf \
   -profile docker \
   --input samplesheet.csv \
   --outdir <OUTDIR>

Warning: Please provide pipeline parameters via the CLI or Nextflow -params-file option. Custom config files including those provided by the -c Nextflow option can be used to provide any configuration except for parameters; see docs.

Credits

UNIFESP_LABMICOBACT/ntm_mterrae_nf was originally written by Abhinav Sharma (@abhi18av).

We thank the following people for their extensive assistance in the development of this pipeline: Luciano Antonio Digiampietri (@digiampietri), Edson Filho Machado Silvia (@edsonmachado) and Emilyn Costa Conceicao (@emilyncosta)

Contributions and Support

If you would like to contribute to this pipeline, please see the contributing guidelines.

Citations

This pipeline is part of the following study: Romagnoli et al. 2024. Description of new species of Mycobacterium terrae complex isolated from sewage at the São Paulo Zoological Park Foundation in Brazil https://www.frontiersin.org/articles/10.3389/fmicb.2024.1335985

An extensive list of references for the tools used by the pipeline can be found in the CITATIONS.md file.

This pipeline uses code and infrastructure developed and maintained by the nf-core community, reused here under the MIT license.

The nf-core framework for community-curated bioinformatics pipelines.

Philip Ewels, Alexander Peltzer, Sven Fillinger, Harshil Patel, Johannes Alneberg, Andreas Wilm, Maxime Ulysse Garcia, Paolo Di Tommaso & Sven Nahnsen.

Nat Biotechnol. 2020 Feb 13. doi: 10.1038/s41587-020-0439-x.