diff --git a/packages/eui/changelogs/upcoming/8844.md b/packages/eui/changelogs/upcoming/8844.md
new file mode 100644
index 00000000000..72063c92367
--- /dev/null
+++ b/packages/eui/changelogs/upcoming/8844.md
@@ -0,0 +1,4 @@
+**Bug fixes**
+
+- Fixed wrong initialization options on `EUI_VIS_COLOR_STORE` which resulted in partially wrong initial color values for static `euiPalette{name}` functions (e.g. `euiPaletteForTemperature`)
+
diff --git a/packages/eui/src/services/color/eui_palettes.test.ts b/packages/eui/src/services/color/eui_palettes.test.ts
index f87214e1a3e..6e91475deab 100644
--- a/packages/eui/src/services/color/eui_palettes.test.ts
+++ b/packages/eui/src/services/color/eui_palettes.test.ts
@@ -93,6 +93,13 @@ describe('euiPaletteForDarkBackground', () => {
 });
 
 describe('euiPaletteForStatus', () => {
+  it('should return correct colors', () => {
+    const colors = colorVis;
+    const resultLong = euiPaletteForStatus(6, { colors });
+
+    expect(resultLong[2]).toEqual('#c8e3b8');
+  });
+
   it('should return custom colors', () => {
     const customColor = '#00ff00';
     const colors = {
@@ -107,6 +114,13 @@ describe('euiPaletteForStatus', () => {
 });
 
 describe('euiPaletteForTemperature', () => {
+  it('should return correct colors', () => {
+    const colors = colorVis;
+    const resultLong = euiPaletteForTemperature(6, { colors });
+
+    expect(resultLong[2]).toEqual('#e8f1ff');
+  });
+
   it('should return custom colors', () => {
     const customColor = '#00ff00';
     const colors = {
@@ -121,6 +135,13 @@ describe('euiPaletteForTemperature', () => {
 });
 
 describe('euiPaletteComplementary', () => {
+  it('should return correct colors', () => {
+    const colors = colorVis;
+    const resultLong = euiPaletteComplementary(6, { colors });
+
+    expect(resultLong[2]).toEqual('#c4dcfd');
+  });
+
   it('should return custom colors', () => {
     const customColor = '#00ff00';
     const colors = {
@@ -135,6 +156,13 @@ describe('euiPaletteComplementary', () => {
 });
 
 describe('euiPaletteRed', () => {
+  it('should return correct colors', () => {
+    const colors = colorVis;
+    const resultLong = euiPaletteRed(6, { colors });
+
+    expect(resultLong[2]).toEqual('#feb7b0');
+  });
+
   it('should return custom colors', () => {
     const customColor = '#00ff00';
     const colors = {
@@ -149,6 +177,13 @@ describe('euiPaletteRed', () => {
 });
 
 describe('euiPaletteGreen', () => {
+  it('should return correct colors', () => {
+    const colors = colorVis;
+    const resultLong = euiPaletteGreen(6, { colors });
+
+    expect(resultLong[2]).toEqual('#9fdfc6');
+  });
+
   it('should return custom colors', () => {
     const customColor = '#00ff00';
     const colors = {
@@ -163,6 +198,13 @@ describe('euiPaletteGreen', () => {
 });
 
 describe('euiPaletteSkyBlue', () => {
+  it('should return correct colors', () => {
+    const colors = colorVis;
+    const resultLong = euiPaletteSkyBlue(6, { colors });
+
+    expect(resultLong[2]).toEqual('#a6d8ec');
+  });
+
   it('should return custom colors', () => {
     const customColor = '#00ff00';
     const colors = {
@@ -177,6 +219,13 @@ describe('euiPaletteSkyBlue', () => {
 });
 
 describe('euiPaletteYellow', () => {
+  it('should return correct colors', () => {
+    const colors = colorVis;
+    const resultLong = euiPaletteYellow(6, { colors });
+
+    expect(resultLong[2]).toEqual('#f9d290');
+  });
+
   it('should return custom colors', () => {
     const customColor = '#00ff00';
     const colors = {
@@ -191,6 +240,13 @@ describe('euiPaletteYellow', () => {
 });
 
 describe('euiPaletteOrange', () => {
+  it('should return correct colors', () => {
+    const colors = colorVis;
+    const resultLong = euiPaletteOrange(6, { colors });
+
+    expect(resultLong[2]).toEqual('#ffc1a1');
+  });
+
   it('should return custom colors', () => {
     const customColor = '#00ff00';
     const colors = {
@@ -205,6 +261,13 @@ describe('euiPaletteOrange', () => {
 });
 
 describe('euiPaletteCool', () => {
+  it('should return correct colors', () => {
+    const colors = colorVis;
+    const resultLong = euiPaletteCool(6, { colors });
+
+    expect(resultLong[2]).toEqual('#a8caff');
+  });
+
   it('should return custom colors', () => {
     const customColor = '#00ff00';
     const colors = {
@@ -219,6 +282,13 @@ describe('euiPaletteCool', () => {
 });
 
 describe('euiPaletteWarm', () => {
+  it('should return correct colors', () => {
+    const colors = colorVis;
+    const resultLong = euiPaletteWarm(6, { colors });
+
+    expect(resultLong[2]).toEqual('#ffafa6');
+  });
+
   it('should return custom colors', () => {
     const customColor = '#00ff00';
     const colors = {
@@ -233,6 +303,13 @@ describe('euiPaletteWarm', () => {
 });
 
 describe('euiPaletteGray', () => {
+  it('should return correct colors', () => {
+    const colors = colorVis;
+    const resultLong = euiPaletteGray(6, { colors });
+
+    expect(resultLong[2]).toEqual('#7b8aa4');
+  });
+
   it('should return custom colors', () => {
     const customColor = '#00ff00';
     const colors = {
diff --git a/packages/eui/src/services/color/vis_color_store.ts b/packages/eui/src/services/color/vis_color_store.ts
index f5dfb1ebb31..732022d8c9d 100644
--- a/packages/eui/src/services/color/vis_color_store.ts
+++ b/packages/eui/src/services/color/vis_color_store.ts
@@ -11,4 +11,4 @@ import { colorVis } from '@elastic/eui-theme-borealis';
 
 // initialsetup of Vis color storage with default colors
 export const EUI_VIS_COLOR_STORE: _EuiVisColorStore =
-  EuiVisColorStore.getInstance(colorVis, true);
+  EuiVisColorStore.getInstance(colorVis, false);