diff --git a/easybuild/easyconfigs/b/Biopython/Biopython-1.76-foss-2023a-Python-2.7.18.eb b/easybuild/easyconfigs/b/Biopython/Biopython-1.76-foss-2023a-Python-2.7.18.eb
new file mode 100644
index 000000000000..d063a9d25fb2
--- /dev/null
+++ b/easybuild/easyconfigs/b/Biopython/Biopython-1.76-foss-2023a-Python-2.7.18.eb
@@ -0,0 +1,43 @@
+easyblock = 'PythonPackage'
+
+name = 'Biopython'
+version = '1.76'
+versionsuffix = '-Python-%(pyver)s'
+
+homepage = 'https://www.biopython.org'
+description = """Biopython is a set of freely available tools for biological
+ computation written in Python by an international team of developers. It is
+ a distributed collaborative effort to develop Python libraries and
+ applications which address the needs of current and future work in
+ bioinformatics. """
+
+toolchain = {'name': 'foss', 'version': '2023a'}
+
+source_urls = ['https://biopython.org/DIST']
+sources = [SOURCELOWER_TAR_GZ]
+checksums = ['3873cb98dad5e28d5e3f2215a012565345a398d3d2c4eebf7cd701757b828c72']
+
+dependencies = [
+    ('Python', '2.7.18'),
+    ('numpy', '1.16.6', versionsuffix),
+]
+
+download_dep_fail = True
+use_pip = True
+sanity_pip_check = True
+
+# Run only tests that don't require internet connection
+runtest = 'python setup.py test --offline'
+
+sanity_check_paths = {
+    'files': [],
+    'dirs': ['lib/python%(pyshortver)s/site-packages/Bio',
+             'lib/python%(pyshortver)s/site-packages/BioSQL']
+}
+
+# extra check to ensure numpy dependency is available
+sanity_check_commands = ["python -c 'import Bio.MarkovModel'"]
+
+options = {'modulename': 'Bio'}
+
+moduleclass = 'bio'