diff --git a/src/coreclr/interpreter/compiler.cpp b/src/coreclr/interpreter/compiler.cpp
index d61bf5fbe81840..69687399a5af8e 100644
--- a/src/coreclr/interpreter/compiler.cpp
+++ b/src/coreclr/interpreter/compiler.cpp
@@ -2854,6 +2854,27 @@ void InterpCompiler::EmitPushLdvirtftn(int thisVar, CORINFO_RESOLVED_TOKEN* pRes
     m_pLastNewIns->info.pCallInfo->pCallArgs = callArgs;
 }
 
+void InterpCompiler::EmitCalli(bool isTailCall, void* calliCookie, int callIFunctionPointerVar, CORINFO_SIG_INFO* callSiteSig)
+{
+    AddIns(isTailCall ? INTOP_CALLI_TAIL : INTOP_CALLI);
+    m_pLastNewIns->data[0] = GetDataItemIndex(calliCookie);
+    // data[1] is set to 1 if the calli is calling a pinvoke, 0 otherwise
+    bool suppressGCTransition = false;
+    CorInfoCallConv callConv = (CorInfoCallConv)(callSiteSig->callConv & IMAGE_CEE_CS_CALLCONV_MASK);
+    bool isPInvoke = (callConv != CORINFO_CALLCONV_DEFAULT && callConv != CORINFO_CALLCONV_VARARG);
+    if (isPInvoke)
+    {
+        if (m_compHnd->pInvokeMarshalingRequired(NULL, callSiteSig))
+        {
+            BADCODE("PInvoke marshalling for calli is not supported in interpreted code");
+        }
+        m_compHnd->getUnmanagedCallConv(nullptr, callSiteSig, &suppressGCTransition);
+    }
+    m_pLastNewIns->data[1] = (suppressGCTransition ? (int32_t)CalliFlags::SuppressGCTransition : 0) |
+                             (isPInvoke ? (int32_t)CalliFlags::PInvoke : 0);
+    m_pLastNewIns->SetSVars2(CALL_ARGS_SVAR, callIFunctionPointerVar);
+}
+
 void InterpCompiler::EmitCall(CORINFO_RESOLVED_TOKEN* pConstrainedToken, bool readonly, bool tailcall, bool newObj, bool isCalli)
 {
     uint32_t token = getU4LittleEndian(m_ip + 1);
@@ -3259,9 +3280,7 @@ void InterpCompiler::EmitCall(CORINFO_RESOLVED_TOKEN* pConstrainedToken, bool re
             }
             else if (isCalli)
             {
-                AddIns(tailcall ? INTOP_CALLI_TAIL : INTOP_CALLI);
-                m_pLastNewIns->data[0] = GetDataItemIndex(calliCookie);
-                m_pLastNewIns->SetSVars2(CALL_ARGS_SVAR, callIFunctionPointerVar);
+                EmitCalli(tailcall, calliCookie, callIFunctionPointerVar, &callInfo.sig);
             }
             else
             {
@@ -3318,9 +3337,7 @@ void InterpCompiler::EmitCall(CORINFO_RESOLVED_TOKEN* pConstrainedToken, bool re
 
             calliCookie = m_compHnd->GetCookieForInterpreterCalliSig(&callInfo.sig);
 
-            AddIns(tailcall ? INTOP_CALLI_TAIL : INTOP_CALLI);
-            m_pLastNewIns->data[0] = GetDataItemIndex(calliCookie);
-            m_pLastNewIns->SetSVars2(CALL_ARGS_SVAR, codePointerLookupResult);
+            EmitCalli(tailcall, calliCookie, codePointerLookupResult, &callInfo.sig);
             break;
         }
         case CORINFO_VIRTUALCALL_VTABLE:
@@ -3353,9 +3370,7 @@ void InterpCompiler::EmitCall(CORINFO_RESOLVED_TOKEN* pConstrainedToken, bool re
 
                 calliCookie = m_compHnd->GetCookieForInterpreterCalliSig(&callInfo.sig);
 
-                AddIns(tailcall ? INTOP_CALLI_TAIL : INTOP_CALLI);
-                m_pLastNewIns->data[0] = GetDataItemIndex(calliCookie);
-                m_pLastNewIns->SetSVars2(CALL_ARGS_SVAR, synthesizedLdvirtftnPtrVar);
+                EmitCalli(tailcall, calliCookie, synthesizedLdvirtftnPtrVar, &callInfo.sig);
             }
             else
             {
diff --git a/src/coreclr/interpreter/compiler.h b/src/coreclr/interpreter/compiler.h
index f8c63336cea119..6c7144ec3dc4d5 100644
--- a/src/coreclr/interpreter/compiler.h
+++ b/src/coreclr/interpreter/compiler.h
@@ -711,6 +711,7 @@ class InterpCompiler
     void    EmitShiftOp(int32_t opBase);
     void    EmitCompareOp(int32_t opBase);
     void    EmitCall(CORINFO_RESOLVED_TOKEN* pConstrainedToken, bool readonly, bool tailcall, bool newObj, bool isCalli);
+    void    EmitCalli(bool isTailCall, void* calliCookie, int callIFunctionPointerVar, CORINFO_SIG_INFO* callSiteSig);
     bool    EmitNamedIntrinsicCall(NamedIntrinsic ni, CORINFO_CLASS_HANDLE clsHnd, CORINFO_METHOD_HANDLE method, CORINFO_SIG_INFO sig);
     void    EmitLdind(InterpType type, CORINFO_CLASS_HANDLE clsHnd, int32_t offset);
     void    EmitStind(InterpType type, CORINFO_CLASS_HANDLE clsHnd, int32_t offset, bool reverseSVarOrder);
diff --git a/src/coreclr/interpreter/inc/interpretershared.h b/src/coreclr/interpreter/inc/interpretershared.h
index 74af7e8ab0ee3a..d9e79f3bffea57 100644
--- a/src/coreclr/interpreter/inc/interpretershared.h
+++ b/src/coreclr/interpreter/inc/interpretershared.h
@@ -171,4 +171,11 @@ enum class PInvokeCallFlags : int32_t
     SuppressGCTransition = 1 << 1, // The pinvoke is marked by the SuppressGCTransition attribute
 };
 
+enum class CalliFlags : int32_t
+{
+    None = 0,
+    SuppressGCTransition = 1 << 1, // The call is marked by the SuppressGCTransition attribute
+    PInvoke = 1 << 2, // The call is a PInvoke call
+};
+
 #endif
diff --git a/src/coreclr/interpreter/inc/intops.def b/src/coreclr/interpreter/inc/intops.def
index 3a6b9754d8b46e..f8ca4befbc9a42 100644
--- a/src/coreclr/interpreter/inc/intops.def
+++ b/src/coreclr/interpreter/inc/intops.def
@@ -358,7 +358,7 @@ OPDEF(INTOP_LDFLDA, "ldflda", 4, 1, 1, InterpOpInt)
 // Calls
 OPDEF(INTOP_CALL, "call", 4, 1, 1, InterpOpMethodHandle)
 OPDEF(INTOP_CALLDELEGATE, "call.delegate", 4, 1, 1, InterpOpMethodHandle)
-OPDEF(INTOP_CALLI, "calli", 5, 1, 2, InterpOpLdPtr)
+OPDEF(INTOP_CALLI, "calli", 6, 1, 2, InterpOpLdPtr)
 OPDEF(INTOP_CALLVIRT, "callvirt", 4, 1, 1, InterpOpMethodHandle)
 OPDEF(INTOP_CALL_PINVOKE, "call.pinvoke", 6, 1, 1, InterpOpMethodHandle) // inlined (no marshaling wrapper) pinvokes only
 OPDEF(INTOP_NEWOBJ, "newobj", 5, 1, 1, InterpOpMethodHandle)
@@ -367,7 +367,7 @@ OPDEF(INTOP_NEWOBJ_VT, "newobj.vt", 5, 1, 1, InterpOpMethodHandle)
 
 // Tail calls
 OPDEF(INTOP_CALL_TAIL, "call.tail", 4, 1, 1, InterpOpMethodHandle)
-OPDEF(INTOP_CALLI_TAIL, "calli", 5, 1, 2, InterpOpLdPtr)
+OPDEF(INTOP_CALLI_TAIL, "calli.tail", 6, 1, 2, InterpOpLdPtr)
 OPDEF(INTOP_CALLVIRT_TAIL, "callvirt.tail", 4, 1, 1, InterpOpMethodHandle)
 
 // The following helper call instructions exist in 2 variants, one for normal methods, and one for cases where a shared generic lookup is needed.
diff --git a/src/coreclr/vm/interpexec.cpp b/src/coreclr/vm/interpexec.cpp
index 040e366456a2b9..e5bd23c538a2cf 100644
--- a/src/coreclr/vm/interpexec.cpp
+++ b/src/coreclr/vm/interpexec.cpp
@@ -13,13 +13,14 @@
 
 // Call invoker helpers provided by platform.
 void InvokeManagedMethod(MethodDesc *pMD, int8_t *pArgs, int8_t *pRet, PCODE target);
-void InvokeUnmanagedMethod(MethodDesc *targetMethod, int8_t *stack, InterpMethodContextFrame *pFrame, int32_t callArgsOffset, int32_t returnOffset, PCODE callTarget);
-void InvokeCalliStub(PCODE ftn, void* stubHeaderTemplate, int8_t *pArgs, int8_t *pRet);
+void InvokeUnmanagedMethod(MethodDesc *targetMethod, int8_t *pArgs, int8_t *pRet, PCODE callTarget);
+void InvokeCalliStub(PCODE ftn, void* cookie, int8_t *pArgs, int8_t *pRet);
+void InvokeUnmanagedCalli(PCODE ftn, void *cookie, int8_t *pArgs, int8_t *pRet);
 void InvokeDelegateInvokeMethod(MethodDesc *pMDDelegateInvoke, int8_t *pArgs, int8_t *pRet, PCODE target);
 
 // Use the NOINLINE to ensure that the InlinedCallFrame in this method is a lower stack address than any InterpMethodContextFrame values.
 NOINLINE
-void InvokeUnmanagedMethodWithTransition(MethodDesc *targetMethod, int8_t *stack, InterpMethodContextFrame *pFrame, int32_t callArgsOffset, int32_t returnOffset, PCODE callTarget)
+void InvokeUnmanagedMethodWithTransition(MethodDesc *targetMethod, int8_t *stack, InterpMethodContextFrame *pFrame, int8_t *pArgs, int8_t *pRet, PCODE callTarget)
 {
     InlinedCallFrame inlinedCallFrame;
     inlinedCallFrame.m_pCallerReturnAddress = (TADDR)pFrame->ip;
@@ -32,12 +33,38 @@ void InvokeUnmanagedMethodWithTransition(MethodDesc *targetMethod, int8_t *stack
     {
         GCX_PREEMP();
         // WASM-TODO: Handle unmanaged calling conventions
-        InvokeManagedMethod(targetMethod, stack + callArgsOffset, stack + returnOffset, callTarget);
+        InvokeManagedMethod(targetMethod, pArgs, pRet, callTarget);
     }
 
     inlinedCallFrame.Pop();
 }
 
+NOINLINE
+void InvokeUnmanagedCalliWithTransition(PCODE ftn, void *cookie, int8_t *stack, InterpMethodContextFrame *pFrame, int8_t *pArgs, int8_t *pRet)
+{
+    CONTRACTL
+    {
+        THROWS;
+        MODE_COOPERATIVE;
+        PRECONDITION(CheckPointer((void*)ftn));
+        PRECONDITION(CheckPointer(cookie));
+    }
+    CONTRACTL_END
+
+    InlinedCallFrame inlinedCallFrame;
+    inlinedCallFrame.m_pCallerReturnAddress = (TADDR)pFrame->ip;
+    inlinedCallFrame.m_pCallSiteSP = pFrame;
+    inlinedCallFrame.m_pCalleeSavedFP = (TADDR)stack;
+    inlinedCallFrame.m_pThread = GetThread();
+    inlinedCallFrame.m_Datum = NULL;
+    inlinedCallFrame.Push();
+    {
+        GCX_PREEMP();
+        InvokeUnmanagedCalli(ftn, cookie, pArgs, pRet);
+    }
+    inlinedCallFrame.Pop();
+}
+
 #ifndef TARGET_WASM
 #include "callstubgenerator.h"
 
@@ -96,9 +123,9 @@ void InvokeManagedMethod(MethodDesc *pMD, int8_t *pArgs, int8_t *pRet, PCODE tar
     pHeader->Invoke(pHeader->Routines, pArgs, pRet, pHeader->TotalStackSize);
 }
 
-void InvokeUnmanagedMethod(MethodDesc *targetMethod, int8_t *stack, InterpMethodContextFrame *pFrame, int32_t callArgsOffset, int32_t returnOffset, PCODE callTarget)
+void InvokeUnmanagedMethod(MethodDesc *targetMethod, int8_t *pArgs, int8_t *pRet, PCODE callTarget)
 {
-    InvokeManagedMethod(targetMethod, stack + callArgsOffset, stack + returnOffset, callTarget);
+    InvokeManagedMethod(targetMethod, pArgs, pRet, callTarget);
 }
 
 void InvokeDelegateInvokeMethod(MethodDesc *pMDDelegateInvoke, int8_t *pArgs, int8_t *pRet, PCODE target)
@@ -129,6 +156,29 @@ void InvokeDelegateInvokeMethod(MethodDesc *pMDDelegateInvoke, int8_t *pArgs, in
     pHeader->Invoke(pHeader->Routines, pArgs, pRet, pHeader->TotalStackSize);
 }
 
+void InvokeUnmanagedCalli(PCODE ftn, void *cookie, int8_t *pArgs, int8_t *pRet)
+{
+    CONTRACTL
+    {
+        THROWS;
+        MODE_ANY;
+        PRECONDITION(CheckPointer((void*)ftn));
+        PRECONDITION(CheckPointer(cookie));
+    }
+    CONTRACTL_END
+
+    // CallStubHeaders encode their destination addresses in the Routines array, so they need to be
+    // copied to a local buffer before we can actually set their target address.
+    CallStubHeader* stubHeaderTemplate = (CallStubHeader*)cookie;
+    size_t templateSize = stubHeaderTemplate->GetSize();
+    uint8_t* actualCallStub = (uint8_t*)alloca(templateSize);
+    memcpy(actualCallStub, stubHeaderTemplate, templateSize);
+    CallStubHeader *pHeader = (CallStubHeader*)actualCallStub;
+    pHeader->SetTarget(ftn); // The method to call
+
+    pHeader->Invoke(pHeader->Routines, pArgs, pRet, pHeader->TotalStackSize);
+}
+
 void InvokeCalliStub(PCODE ftn, void *cookie, int8_t *pArgs, int8_t *pRet)
 {
     CONTRACTL
@@ -2117,15 +2167,31 @@ void InterpExecMethod(InterpreterFrame *pInterpreterFrame, InterpMethodContextFr
                     callArgsOffset = ip[2];
                     int32_t calliFunctionPointerVar = ip[3];
                     int32_t calliCookie = ip[4];
+                    int32_t flags = ip[5];
 
                     void* cookie = pMethod->pDataItems[calliCookie];
-                    ip += 5;
+                    ip += 6;
 
                     // Save current execution state for when we return from called method
                     pFrame->ip = ip;
 
                     // Interpreter-FIXME: isTailcall
-                    InvokeCalliStub(LOCAL_VAR(calliFunctionPointerVar, PCODE), cookie, stack + callArgsOffset, stack + returnOffset);
+                    if (flags & (int32_t)CalliFlags::PInvoke)
+                    {
+                        if (flags & (int32_t)CalliFlags::SuppressGCTransition)
+                        {
+                            InvokeUnmanagedCalli(LOCAL_VAR(calliFunctionPointerVar, PCODE), cookie, stack + callArgsOffset, stack + returnOffset);
+                        }
+                        else
+                        {
+                            InvokeUnmanagedCalliWithTransition(LOCAL_VAR(calliFunctionPointerVar, PCODE), cookie, stack, pFrame, stack + callArgsOffset, stack + returnOffset);
+                        }
+                    }
+                    else
+                    {
+                        InvokeCalliStub(LOCAL_VAR(calliFunctionPointerVar, PCODE), cookie, stack + callArgsOffset, stack + returnOffset);
+                    }
+
                     break;
                 }
 
@@ -2152,11 +2218,11 @@ void InterpExecMethod(InterpreterFrame *pInterpreterFrame, InterpMethodContextFr
 
                     if (flags & (int32_t)PInvokeCallFlags::SuppressGCTransition)
                     {
-                        InvokeUnmanagedMethod(targetMethod, stack, pFrame, callArgsOffset, returnOffset, callTarget);
+                        InvokeUnmanagedMethod(targetMethod, stack + callArgsOffset, stack + returnOffset, callTarget);
                     }
                     else
                     {
-                        InvokeUnmanagedMethodWithTransition(targetMethod, stack, pFrame, callArgsOffset, returnOffset, callTarget);
+                        InvokeUnmanagedMethodWithTransition(targetMethod, stack, pFrame, stack + callArgsOffset, stack + returnOffset, callTarget);
                     }
 
                     break;
diff --git a/src/coreclr/vm/wasm/helpers.cpp b/src/coreclr/vm/wasm/helpers.cpp
index a85456b76413d5..5db0695c61eebf 100644
--- a/src/coreclr/vm/wasm/helpers.cpp
+++ b/src/coreclr/vm/wasm/helpers.cpp
@@ -486,7 +486,7 @@ void InvokeManagedMethod(MethodDesc *pMD, int8_t *pArgs, int8_t *pRet, PCODE tar
     PORTABILITY_ASSERT("Attempted to execute non-interpreter code from interpreter on wasm, this is not yet implemented");
 }
 
-void InvokeUnmanagedMethod(MethodDesc *targetMethod, int8_t *stack, InterpMethodContextFrame *pFrame, int32_t callArgsOffset, int32_t returnOffset, PCODE callTarget)
+void InvokeUnmanagedMethod(MethodDesc *targetMethod, int8_t *pArgs, int8_t *pRet, PCODE callTarget)
 {
     PORTABILITY_ASSERT("Attempted to execute unmanaged code from interpreter on wasm, this is not yet implemented");
 }
@@ -499,6 +499,11 @@ void InvokeCalliStub(PCODE ftn, void* cookie, int8_t *pArgs, int8_t *pRet)
     ((void(*)(PCODE, int8_t*, int8_t*))cookie)(ftn, pArgs, pRet);
 }
 
+void InvokeUnmanagedCalli(PCODE ftn, void *cookie, int8_t *pArgs, int8_t *pRet)
+{
+    PORTABILITY_ASSERT("Attempted to execute unmanaged calli from interpreter on wasm, this is not yet implemented");
+}
+
 void InvokeDelegateInvokeMethod(MethodDesc *pMDDelegateInvoke, int8_t *pArgs, int8_t *pRet, PCODE target)
 {
     PORTABILITY_ASSERT("Attempted to execute non-interpreter code from interpreter on wasm, this is not yet implemented");