diff --git a/src/coreclr/tools/aot/crossgen2/crossgen2.props b/src/coreclr/tools/aot/crossgen2/crossgen2.props
index 2ec464eaa54bd5..b2ce7bf75992d7 100644
--- a/src/coreclr/tools/aot/crossgen2/crossgen2.props
+++ b/src/coreclr/tools/aot/crossgen2/crossgen2.props
@@ -55,12 +55,14 @@
     <Content Include="$(CoreCLRArtifactsPath)/$(CrossHostArch)/$(JitInterfaceLibraryName)"
       CopyToOutputDirectory="PreserveNewest"
       CopyToPublishDirectory="PreserveNewest"
+      ExcludeFromSingleFile="$(PublishSingleFile)"
       Link="%(FileName)%(Extension)"
       />
 
     <Content Include="$(CoreCLRArtifactsPath)/$(CrossHostArch)/$(_LibPrefix)clrjit_*_$(TargetArchitectureForSharedLibraries)$(_LibSuffix)"
       CopyToOutputDirectory="PreserveNewest"
       CopyToPublishDirectory="PreserveNewest"
+      ExcludeFromSingleFile="$(PublishSingleFile)"
       Link="%(FileName)%(Extension)"
       />
   </ItemGroup>
@@ -69,6 +71,7 @@
     <Content Include="$(RuntimeBinDir)/pgort*.dll"
       CopyToOutputDirectory="PreserveNewest"
       CopyToPublishDirectory="PreserveNewest"
+      ExcludeFromSingleFile="$(PublishSingleFile)"
       Link="%(FileName)%(Extension)"
       Condition="'$(PgoInstrument)' != ''"
       />
@@ -94,6 +97,7 @@
     <Content Include="$(DiaSymReaderTargetArchPath)"
       CopyToOutputDirectory="PreserveNewest"
       CopyToPublishDirectory="PreserveNewest"
+      ExcludeFromSingleFile="$(PublishSingleFile)"
       Link="%(FileName)%(Extension)"
       />
   </ItemGroup>