diff --git a/eng/testing/scenarios/BuildWasmAppsJobsList.txt b/eng/testing/scenarios/BuildWasmAppsJobsList.txt
index 6b41da61285c23..818ff9e8edd3c2 100644
--- a/eng/testing/scenarios/BuildWasmAppsJobsList.txt
+++ b/eng/testing/scenarios/BuildWasmAppsJobsList.txt
@@ -39,6 +39,7 @@ Wasm.Build.Tests.TestAppScenarios.LazyLoadingTests
 Wasm.Build.Tests.TestAppScenarios.LibraryInitializerTests
 Wasm.Build.Tests.TestAppScenarios.SatelliteLoadingTests
 Wasm.Build.Tests.TestAppScenarios.ModuleConfigTests
+Wasm.Build.Tests.TestAppScenarios.MemoryTests
 Wasm.Build.Tests.AspNetCore.SignalRClientTests
 Wasm.Build.Tests.WasmBuildAppTest
 Wasm.Build.Tests.WasmNativeDefaultsTests
diff --git a/src/mono/wasm/Wasm.Build.Tests/TestAppScenarios/MemoryTests.cs b/src/mono/wasm/Wasm.Build.Tests/TestAppScenarios/MemoryTests.cs
new file mode 100644
index 00000000000000..a1f7cd8d72ce93
--- /dev/null
+++ b/src/mono/wasm/Wasm.Build.Tests/TestAppScenarios/MemoryTests.cs
@@ -0,0 +1,37 @@
+// Licensed to the .NET Foundation under one or more agreements.
+// The .NET Foundation licenses this file to you under the MIT license.
+
+using System;
+using System.Collections.Generic;
+using System.Linq;
+using System.Threading.Tasks;
+using Xunit.Abstractions;
+using Xunit;
+
+#nullable enable
+
+namespace Wasm.Build.Tests.TestAppScenarios;
+
+public class MemoryTests : AppTestBase
+{
+    public MemoryTests(ITestOutputHelper output, SharedBuildPerTestClassFixture buildContext)
+        : base(output, buildContext)
+    {
+    }
+
+    [Theory]
+    [InlineData("Release", true)]
+    [InlineData("Release", false)]
+    public async Task AllocateLargeHeapThenRepeatedlyInterop(string config, bool buildNative)
+    {
+        // native build triggers passing value form EmccMaximumHeapSize to MAXIMUM_MEMORY that is set in emscripten
+        // in non-native build EmccMaximumHeapSize does not have an effect, so the test will fail with "out of memory"
+        CopyTestAsset("WasmBasicTestApp", "MemoryTests", "App");
+        string extraArgs = $"-p:EmccMaximumHeapSize=4294901760 -p:WasmBuildNative={buildNative}";
+        BuildProject(config, assertAppBundle: false, extraArgs: extraArgs);
+
+        var result = await RunSdkStyleAppForBuild(new (Configuration: config, TestScenario: "AllocateLargeHeapThenInterop", ExpectedExitCode: buildNative ? 0 : 1));
+        if (!buildNative)
+            Assert.Contains(result.TestOutput, item => item.Contains("Exception System.OutOfMemoryException: Out of memory"));
+    }
+}
diff --git a/src/mono/wasm/testassets/WasmBasicTestApp/App/MemoryTest.cs b/src/mono/wasm/testassets/WasmBasicTestApp/App/MemoryTest.cs
new file mode 100644
index 00000000000000..f59915b0be5eaa
--- /dev/null
+++ b/src/mono/wasm/testassets/WasmBasicTestApp/App/MemoryTest.cs
@@ -0,0 +1,74 @@
+// Licensed to the .NET Foundation under one or more agreements.
+// The .NET Foundation licenses this file to you under the MIT license.
+
+using System;
+using System.IO;
+using System.Text.Json;
+using System.Text;
+using System.Runtime.InteropServices.JavaScript;
+
+public partial class MemoryTest // ?test=AllocateLargeHeapThenInterop
+{
+    [JSImport("countChars", "main.js")]
+    internal static partial int CountChars(string testArray);
+
+    [JSExport]
+    internal static void Run()
+    {
+        // Allocate over 2GB space, 2 621 440 000 bytes
+        const int arrayCnt = 25;
+        int[][] arrayHolder = new int[arrayCnt][];
+        string errors = "";
+        TestOutput.WriteLine("Starting over 2GB array allocation");
+        for (int i = 0; i < arrayCnt; i++)
+        {
+            try
+            {
+                arrayHolder[i] = new int[1024 * 1024 * 25];
+            }
+            catch (Exception ex)
+            {
+                errors += $"Exception {ex} was thrown on i={i}";
+            }
+        }
+        TestOutput.WriteLine("Finished over 2GB array allocation");
+
+        // call a method many times to trigger tier-up optimization
+        string randomString = GenerateRandomString(1000);
+        try
+        {
+            for (int i = 0; i < 10000; i++)
+            {
+                int count = CountChars(randomString);
+                if (count != randomString.Length)
+                    errors += $"CountChars returned {count} instead of {randomString.Length} for {i}-th string.";
+            }
+        }
+        catch (Exception ex)
+        {
+            errors += $"Exception {ex} was thrown when CountChars was called in a loop";
+        }
+        if (!string.IsNullOrEmpty(errors))
+        {
+            TestOutput.WriteLine(errors);
+            throw new Exception(errors);
+        }
+        else
+        {
+            TestOutput.WriteLine("Great success, MemoryTest finished without errors.");
+        }
+    }
+
+    private static Random random = new Random();
+
+    private static string GenerateRandomString(int stringLength)
+    {
+        const string chars = "ABCDEFGHIJKLMNOPQRSTUVWXYZabcdefghijklmnopqrstuvwxyz";
+        var stringBuilder = new StringBuilder(stringLength);
+        for (int i = 0; i < stringLength; i++)
+        {
+            stringBuilder.Append(chars[random.Next(chars.Length)]);
+        }
+        return stringBuilder.ToString();
+    }
+}
diff --git a/src/mono/wasm/testassets/WasmBasicTestApp/App/wwwroot/main.js b/src/mono/wasm/testassets/WasmBasicTestApp/App/wwwroot/main.js
index 0023e28f4c3338..72235f9dc9ac92 100644
--- a/src/mono/wasm/testassets/WasmBasicTestApp/App/wwwroot/main.js
+++ b/src/mono/wasm/testassets/WasmBasicTestApp/App/wwwroot/main.js
@@ -14,6 +14,12 @@ function testOutput(msg) {
     console.log(`TestOutput -> ${msg}`);
 }
 
+function countChars(str) {
+    const length = str.length;
+    testOutput(`JS received str of ${length} length`);
+    return length;
+}
+
 // Prepare base runtime parameters
 dotnet
     .withElementOnExit()
@@ -168,6 +174,13 @@ try {
         case "MaxParallelDownloads":
             exit(0);
             break;
+        case "AllocateLargeHeapThenInterop":
+            setModuleImports('main.js', {
+                countChars
+            });
+            exports.MemoryTest.Run();
+            exit(0);
+            break;
         default:
             console.error(`Unknown test case: ${testCase}`);
             exit(3);