diff --git a/src/Controls/tests/TestCases.HostApp/Issues/Issue33067.cs b/src/Controls/tests/TestCases.HostApp/Issues/Issue33067.cs
new file mode 100644
index 000000000000..2dd1044ec09c
--- /dev/null
+++ b/src/Controls/tests/TestCases.HostApp/Issues/Issue33067.cs
@@ -0,0 +1,80 @@
+using System.Collections.ObjectModel;
+
+namespace Maui.Controls.Sample.Issues;
+
+[Issue(IssueTracker.Github, 33067, "[Windows, Android] ScrollView Content Not Removed When Set to Null", PlatformAffected.Android | PlatformAffected.UWP)]
+
+public class Issue33067 : ContentPage
+{
+	ScrollView _scrollView = null!;
+	Label _originalContent = null!;
+	Button _setNullButton = null!;
+	Button _addContentButton = null!;
+
+	public Issue33067()
+	{
+		CreateUI();
+	}
+
+	void CreateUI()
+	{
+		// Create the original content label
+		_originalContent = new Label
+		{
+			Text = "This is a sample label inside the ScrollView that can be set to null and added back.",
+			Padding = new Thickness(20),
+			AutomationId = "ContentLabel",
+			FontSize = 16
+		};
+
+		// Create the ScrollView
+		_scrollView = new ScrollView
+		{
+			BackgroundColor = Colors.LightGray,
+			HeightRequest = 300,
+			Content = null
+		};
+
+		// Create the "Set Content to Null" button
+		_setNullButton = new Button
+		{
+			Text = "Set Content to Null",
+			AutomationId = "SetNullButton"
+		};
+		_setNullButton.Clicked += OnSetContentNullClicked;
+
+		// Create the "Add Content" button
+		_addContentButton = new Button
+		{
+			Text = "Add Content",
+			AutomationId = "AddContentButton"
+		};
+		_addContentButton.Clicked += OnAddContentClicked;
+
+		// Create the main layout
+		var layout = new VerticalStackLayout
+		{
+			Spacing = 20,
+			Padding = new Thickness(30),
+			Children = { _setNullButton, _addContentButton, _scrollView }
+		};
+
+		// Set the content of the page
+		Content = layout;
+	}
+
+	void OnSetContentNullClicked(object sender, EventArgs e)
+	{
+		// Set ScrollView content to null
+		_scrollView.Content = null;
+	}
+
+	void OnAddContentClicked(object sender, EventArgs e)
+	{
+		// Restore the original content
+		if (_originalContent != null)
+		{
+			_scrollView.Content = _originalContent;
+		}
+	}
+}
\ No newline at end of file
diff --git a/src/Controls/tests/TestCases.Shared.Tests/Tests/Issues/Issue33067.cs b/src/Controls/tests/TestCases.Shared.Tests/Tests/Issues/Issue33067.cs
new file mode 100644
index 000000000000..45692b1ac65e
--- /dev/null
+++ b/src/Controls/tests/TestCases.Shared.Tests/Tests/Issues/Issue33067.cs
@@ -0,0 +1,30 @@
+using NUnit.Framework;
+using UITest.Appium;
+using UITest.Core;
+
+namespace Microsoft.Maui.TestCases.Tests.Issues;
+public class Issue33067 : _IssuesUITest
+{
+	public Issue33067(TestDevice device) : base(device) { }
+
+	public override string Issue => "[Windows, Android] ScrollView Content Not Removed When Set to Null";
+
+	[Test, Order(1)]
+	[Category(UITestCategories.ScrollView)]
+	public void VerifyScrollViewContentShouldNull()
+    {
+        App.WaitForElement("SetNullButton");
+		App.WaitForNoElement("ContentLabel");
+    }
+
+	[Test, Order(2)]
+	[Category(UITestCategories.ScrollView)]
+	public void VerifyScrollViewContentWhenSetToNull()
+	{
+		App.WaitForElement("SetNullButton");
+		App.Tap("AddContentButton");
+		App.WaitForElement("ContentLabel");
+		App.Tap("SetNullButton");
+		App.WaitForNoElement("ContentLabel");
+	}
+}
\ No newline at end of file
diff --git a/src/Core/src/Handlers/ScrollView/ScrollViewHandler.Android.cs b/src/Core/src/Handlers/ScrollView/ScrollViewHandler.Android.cs
index a2ed1e09ec31..6b836a33ac5a 100644
--- a/src/Core/src/Handlers/ScrollView/ScrollViewHandler.Android.cs
+++ b/src/Core/src/Handlers/ScrollView/ScrollViewHandler.Android.cs
@@ -196,15 +196,26 @@ The methods below exist to support inserting/updating the extra padding/margin l
 
 		static void UpdateInsetView(IScrollView scrollView, IScrollViewHandler handler, ICrossPlatformLayout crossPlatformLayout)
 		{
-			if (scrollView.PresentedContent == null || handler.MauiContext == null)
+			if (handler.MauiContext is null)
 			{
 				return;
 			}
 
+			// Find existing inset panel once
+			var currentPaddingLayer = FindInsetPanel(handler);
+
+			// If PresentedContent is null, clean up any existing content and return
+			if (scrollView.PresentedContent is null)
+			{
+				currentPaddingLayer?.RemoveAllViews();
+				return;
+			}
+
 			var nativeContent = scrollView.PresentedContent.ToPlatform(handler.MauiContext);
 
-			if (FindInsetPanel(handler) is ContentViewGroup currentPaddingLayer)
+			if (currentPaddingLayer is not null)
 			{
+				// Only update if content has changed or is missing
 				if (currentPaddingLayer.ChildCount == 0 || currentPaddingLayer.GetChildAt(0) != nativeContent)
 				{
 					currentPaddingLayer.RemoveAllViews();
diff --git a/src/Core/src/Handlers/ScrollView/ScrollViewHandler.Windows.cs b/src/Core/src/Handlers/ScrollView/ScrollViewHandler.Windows.cs
index 1bfe2081418e..8a3214733212 100644
--- a/src/Core/src/Handlers/ScrollView/ScrollViewHandler.Windows.cs
+++ b/src/Core/src/Handlers/ScrollView/ScrollViewHandler.Windows.cs
@@ -125,21 +125,34 @@ The methods below exist to support inserting/updating the padding/margin panel.
 
 		static void UpdateContentPanel(IScrollView scrollView, IScrollViewHandler handler, ICrossPlatformLayout crossPlatformLayout)
 		{
-			if (scrollView.PresentedContent == null || handler.MauiContext == null)
+			if (handler.MauiContext is null)
 			{
 				return;
 			}
 
 			var scrollViewer = handler.PlatformView;
+			var currentPaddingLayer = GetContentPanel(scrollViewer);
+
+			// If PresentedContent is null, clean up any existing content and return
+			if (scrollView.PresentedContent is null)
+			{
+				if (currentPaddingLayer is not null)
+				{
+					currentPaddingLayer.CachedChildren.Clear();
+				}
+				
+				return;
+			}
+
 			var nativeContent = scrollView.PresentedContent.ToPlatform(handler.MauiContext);
 
-			if (GetContentPanel(scrollViewer) is ContentPanel currentPaddingLayer)
+			if (currentPaddingLayer is not null)
 			{
+				// Only update if content has changed or is missing
 				if (currentPaddingLayer.CachedChildren.Count == 0 || currentPaddingLayer.CachedChildren[0] != nativeContent)
 				{
 					currentPaddingLayer.CachedChildren.Clear();
 					currentPaddingLayer.CachedChildren.Add(nativeContent);
-
 				}
 			}
 			else