diff --git a/tests/Aspire.Cli.EndToEnd.Tests/Helpers/CliE2ETestHelpers.cs b/tests/Aspire.Cli.EndToEnd.Tests/Helpers/CliE2ETestHelpers.cs
index b45a2abe90e..c68b623ac1a 100644
--- a/tests/Aspire.Cli.EndToEnd.Tests/Helpers/CliE2ETestHelpers.cs
+++ b/tests/Aspire.Cli.EndToEnd.Tests/Helpers/CliE2ETestHelpers.cs
@@ -139,15 +139,23 @@ internal static Hex1bTerminalInputSequenceBuilder InstallAspireCliFromPullReques
 
     internal static Hex1bTerminalInputSequenceBuilder SourceAspireCliEnvironment(
         this Hex1bTerminalInputSequenceBuilder builder,
-        SequenceCounter counter
+        SequenceCounter counter,
+        bool enablePolyglotSupport = false
         )
     {
         if (OperatingSystem.IsWindows())
         {
             // On Windows, the PowerShell installer already updates the current session's PATH
             // But we still need to set ASPIRE_PLAYGROUND for interactive mode and .NET CLI vars
+            var envVars = "$env:ASPIRE_PLAYGROUND='true'; $env:DOTNET_CLI_TELEMETRY_OPTOUT='true'; $env:DOTNET_SKIP_FIRST_TIME_EXPERIENCE='true'; $env:DOTNET_GENERATE_ASPNET_CERTIFICATE='false'";
+            
+            if (enablePolyglotSupport)
+            {
+                envVars += "; $env:features__polyglotSupportEnabled='true'";
+            }
+            
             return builder
-                .Type("$env:ASPIRE_PLAYGROUND='true'; $env:DOTNET_CLI_TELEMETRY_OPTOUT='true'; $env:DOTNET_SKIP_FIRST_TIME_EXPERIENCE='true'; $env:DOTNET_GENERATE_ASPNET_CERTIFICATE='false'")
+                .Type(envVars)
                 .Enter()
                 .WaitForSuccessPrompt(counter);
         }
@@ -156,8 +164,16 @@ SequenceCounter counter
         // We need to add it to PATH and set environment variables:
         // - ASPIRE_PLAYGROUND=true enables interactive mode
         // - .NET CLI vars suppress telemetry and first-time experience which can cause hangs
+        // - features__polyglotSupportEnabled=true enables polyglot support (Python, Go, Java, Rust)
+        var exportVars = "export PATH=~/.aspire/bin:$PATH ASPIRE_PLAYGROUND=true DOTNET_CLI_TELEMETRY_OPTOUT=true DOTNET_SKIP_FIRST_TIME_EXPERIENCE=true DOTNET_GENERATE_ASPNET_CERTIFICATE=false";
+        
+        if (enablePolyglotSupport)
+        {
+            exportVars += " features__polyglotSupportEnabled=true";
+        }
+        
         return builder
-            .Type("export PATH=~/.aspire/bin:$PATH ASPIRE_PLAYGROUND=true DOTNET_CLI_TELEMETRY_OPTOUT=true DOTNET_SKIP_FIRST_TIME_EXPERIENCE=true DOTNET_GENERATE_ASPNET_CERTIFICATE=false")
+            .Type(exportVars)
             .Enter()
             .WaitForSuccessPrompt(counter);
     }
diff --git a/tests/Aspire.Cli.EndToEnd.Tests/JsReactTemplateTests.cs b/tests/Aspire.Cli.EndToEnd.Tests/JsReactTemplateTests.cs
index 27d6f03fe4b..a84bf34b139 100644
--- a/tests/Aspire.Cli.EndToEnd.Tests/JsReactTemplateTests.cs
+++ b/tests/Aspire.Cli.EndToEnd.Tests/JsReactTemplateTests.cs
@@ -71,7 +71,7 @@ public async Task CreateAndRunJsReactProject()
         if (isCI)
         {
             sequenceBuilder.InstallAspireCliFromPullRequest(prNumber, counter);
-            sequenceBuilder.SourceAspireCliEnvironment(counter);
+            sequenceBuilder.SourceAspireCliEnvironment(counter, enablePolyglotSupport: true);
             sequenceBuilder.VerifyAspireCliVersion(commitSha, counter);
         }
 
diff --git a/tests/Aspire.Cli.EndToEnd.Tests/PolyglotPythonTests.cs b/tests/Aspire.Cli.EndToEnd.Tests/PolyglotPythonTests.cs
index cf6300c5c99..3237e45bea6 100644
--- a/tests/Aspire.Cli.EndToEnd.Tests/PolyglotPythonTests.cs
+++ b/tests/Aspire.Cli.EndToEnd.Tests/PolyglotPythonTests.cs
@@ -54,7 +54,7 @@ public async Task CreatePythonAppHostWithRedisAndRun()
         if (isCI)
         {
             sequenceBuilder.InstallAspireCliFromPullRequest(prNumber, counter);
-            sequenceBuilder.SourceAspireCliEnvironment(counter);
+            sequenceBuilder.SourceAspireCliEnvironment(counter, enablePolyglotSupport: true);
             sequenceBuilder.VerifyAspireCliVersion(commitSha, counter);
         }
 
diff --git a/tests/Aspire.Cli.EndToEnd.Tests/PythonReactTemplateTests.cs b/tests/Aspire.Cli.EndToEnd.Tests/PythonReactTemplateTests.cs
index 31160cd0d83..b30fbe45318 100644
--- a/tests/Aspire.Cli.EndToEnd.Tests/PythonReactTemplateTests.cs
+++ b/tests/Aspire.Cli.EndToEnd.Tests/PythonReactTemplateTests.cs
@@ -71,7 +71,7 @@ public async Task CreateAndRunPythonReactProject()
         if (isCI)
         {
             sequenceBuilder.InstallAspireCliFromPullRequest(prNumber, counter);
-            sequenceBuilder.SourceAspireCliEnvironment(counter);
+            sequenceBuilder.SourceAspireCliEnvironment(counter, enablePolyglotSupport: true);
             sequenceBuilder.VerifyAspireCliVersion(commitSha, counter);
         }