diff --git a/CHANGELOG.md b/CHANGELOG.md
index 9f44817f779..3260ec7a469 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -9,6 +9,7 @@
 * [ENHANCEMENT] StoreGateway: Emit more histogram buckets on the `cortex_querier_storegateway_refetches_per_query` metric. #6570
 * [ENHANCEMENT] Querier: Apply bytes limiter to LabelNames and LabelValuesForLabelNames. #6568
 * [ENHANCEMENT] Query Frontend: Add a `too_many_tenants` reason label value to `cortex_rejected_queries_total` metric to track the rejected query count due to the # of tenant limits. #6569
+* [ENHANCEMENT] Alertmanager: Add receiver validations for msteamsv2 and rocketchat. #6606
 * [BUGFIX] Ingester: Avoid error or early throttling when READONLY ingesters are present in the ring #6517
 * [BUGFIX] Ingester: Fix labelset data race condition. #6573
 * [BUGFIX] Compactor: Cleaner should not put deletion marker for blocks with no-compact marker. #6576
diff --git a/pkg/alertmanager/api.go b/pkg/alertmanager/api.go
index 6f51e7c766b..a4a73d43014 100644
--- a/pkg/alertmanager/api.go
+++ b/pkg/alertmanager/api.go
@@ -52,6 +52,10 @@ var (
 	errPushOverUserKeyFileNotAllowed     = errors.New("setting PushOver user_key_file is not allowed")
 	errPushOverTokenFileNotAllowed       = errors.New("setting PushOver token_file is not allowed")
 	errTelegramBotTokenFileNotAllowed    = errors.New("setting Telegram bot_token_file is not allowed")
+	errMSTeamsWebhookUrlFileNotAllowed   = errors.New("setting MSTeams webhook_url_file is not allowed")
+	errMSTeamsV2WebhookUrlFileNotAllowed = errors.New("setting MSTeamsV2 webhook_url_file is not allowed")
+	errRocketChatTokenIdFileNotAllowed   = errors.New("setting RocketChat token_id_file is not allowed")
+	errRocketChatTokenFileNotAllowed     = errors.New("setting RocketChat token_file is not allowed")
 )
 
 // UserConfig is used to communicate a users alertmanager configs
@@ -383,6 +387,18 @@ func validateAlertmanagerConfig(cfg interface{}) error {
 		if err := validateTelegramConfig(v.Interface().(config.TelegramConfig)); err != nil {
 			return err
 		}
+	case reflect.TypeOf(config.MSTeamsConfig{}):
+		if err := validateMSTeamsConfig(v.Interface().(config.MSTeamsConfig)); err != nil {
+			return err
+		}
+	case reflect.TypeOf(config.MSTeamsV2Config{}):
+		if err := validateMSTeamsV2Config(v.Interface().(config.MSTeamsV2Config)); err != nil {
+			return err
+		}
+	case reflect.TypeOf(config.RocketchatConfig{}):
+		if err := validateRocketChatConfig(v.Interface().(config.RocketchatConfig)); err != nil {
+			return err
+		}
 	}
 
 	// If the input config is a struct, recursively iterate on all fields.
@@ -540,3 +556,35 @@ func validateTelegramConfig(cfg config.TelegramConfig) error {
 	}
 	return nil
 }
+
+// validateMSTeamsConfig validates the MSTeams Config and returns an error if it contains
+// settings not allowed by Cortex.
+func validateMSTeamsConfig(cfg config.MSTeamsConfig) error {
+	if cfg.WebhookURLFile != "" {
+		return errMSTeamsWebhookUrlFileNotAllowed
+	}
+	return nil
+}
+
+// validateMSTeamsV2Config validates the MSTeamsV2 Config and returns an error if it contains
+// settings not allowed by Cortex.
+func validateMSTeamsV2Config(cfg config.MSTeamsV2Config) error {
+	if cfg.WebhookURLFile != "" {
+		return errMSTeamsV2WebhookUrlFileNotAllowed
+	}
+	return nil
+}
+
+// validateRocketChatConfig validates the RocketChat Config and returns an error if it contains
+// settings not allowed by Cortex.
+func validateRocketChatConfig(cfg config.RocketchatConfig) error {
+	if cfg.TokenIDFile != "" {
+		return errRocketChatTokenIdFileNotAllowed
+	}
+
+	if cfg.TokenFile != "" {
+		return errRocketChatTokenFileNotAllowed
+	}
+
+	return nil
+}
diff --git a/pkg/alertmanager/api_test.go b/pkg/alertmanager/api_test.go
index bdd9c6504de..e70af952478 100644
--- a/pkg/alertmanager/api_test.go
+++ b/pkg/alertmanager/api_test.go
@@ -649,6 +649,60 @@ alertmanager_config: |
 `,
 			err: errors.Wrap(errTelegramBotTokenFileNotAllowed, "error validating Alertmanager config"),
 		},
+		{
+			name: "Should return error if MSTeams webhook_url_file is set",
+			cfg: `
+alertmanager_config: |
+  receivers:
+    - name: default-receiver
+      msteams_configs:
+        - webhook_url_file: /urlFile
+  route:
+    receiver: 'default-receiver'
+`,
+			err: errors.Wrap(errMSTeamsWebhookUrlFileNotAllowed, "error validating Alertmanager config"),
+		},
+		{
+			name: "Should return error if MSTeamsV2 webhook_url_file is set",
+			cfg: `
+alertmanager_config: |
+  receivers:
+    - name: default-receiver
+      msteamsv2_configs:
+        - webhook_url_file: /urlFile
+  route:
+    receiver: 'default-receiver'
+`,
+			err: errors.Wrap(errMSTeamsV2WebhookUrlFileNotAllowed, "error validating Alertmanager config"),
+		},
+		{
+			name: "Should return error if RocketChat token_id_file is set",
+			cfg: `
+alertmanager_config: |
+  receivers:
+    - name: default-receiver
+      rocketchat_configs:
+        - token_id_file: /tokenIdFile
+          token: 'token'
+  route:
+    receiver: 'default-receiver'
+`,
+			err: errors.Wrap(errRocketChatTokenIdFileNotAllowed, "error validating Alertmanager config"),
+		},
+		{
+			name: "Should return error if RocketChat token_file is set",
+			cfg: `
+alertmanager_config: |
+  receivers:
+    - name: default-receiver
+      rocketchat_configs:
+        - token_file: /tokenFile
+          token_id: 'tokenId'
+  route:
+    receiver: 'default-receiver'
+`,
+			err: errors.Wrap(errRocketChatTokenFileNotAllowed, "error validating Alertmanager config"),
+		},
 	}
 
 	limits := &mockAlertManagerLimits{}