diff --git a/CHANGELOG.md b/CHANGELOG.md
index a81c8e682b3..f0a2c2ffe23 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -23,6 +23,7 @@
   * `cortex_prometheus_notifications_queue_capacity`
   * `cortex_prometheus_notifications_alertmanagers_discovered`
 * [ENHANCEMENT] Added `-ingester.flush-on-shutdown-with-wal-enabled` option to enable chunks flushing even when WAL is enabled. #2780
+* [ENHANCEMENT] Query-tee: Support for custom API prefix by using `-server.path-prefix` option. #2814
 * [BUGFIX] Fixed a bug in the index intersect code causing storage to return more chunks/series than required. #2796
 * [BUGFIX] Fixed the number of reported keys in the background cache queue. #2764
 * [BUGFIX] Fix race in processing of headers in sharded queries. #2762
diff --git a/cmd/query-tee/main.go b/cmd/query-tee/main.go
index f8ef82e6914..ac0d6438a30 100644
--- a/cmd/query-tee/main.go
+++ b/cmd/query-tee/main.go
@@ -17,12 +17,14 @@ type Config struct {
 	ServerMetricsPort int
 	LogLevel          logging.Level
 	ProxyConfig       querytee.ProxyConfig
+	PathPrefix        string
 }
 
 func main() {
 	// Parse CLI flags.
 	cfg := Config{}
 	flag.IntVar(&cfg.ServerMetricsPort, "server.metrics-port", 9900, "The port where metrics are exposed.")
+	flag.StringVar(&cfg.PathPrefix, "server.path-prefix", "", "Prefix for API paths (query-tee will accept Prometheus API calls at <prefix>/api/v1/...)")
 	cfg.LogLevel.RegisterFlags(flag.CommandLine)
 	cfg.ProxyConfig.RegisterFlags(flag.CommandLine)
 	flag.Parse()
@@ -42,7 +44,7 @@ func main() {
 	}
 
 	// Run the proxy.
-	proxy, err := querytee.NewProxy(cfg.ProxyConfig, util.Logger, cortexReadRoutes(), registry)
+	proxy, err := querytee.NewProxy(cfg.ProxyConfig, util.Logger, cortexReadRoutes(cfg.PathPrefix), registry)
 	if err != nil {
 		level.Error(util.Logger).Log("msg", "Unable to initialize the proxy", "err", err.Error())
 		os.Exit(1)
@@ -56,16 +58,21 @@ func main() {
 	proxy.Await()
 }
 
-func cortexReadRoutes() []querytee.Route {
+func cortexReadRoutes(prefix string) []querytee.Route {
+	// Strip trailing slashes.
+	for len(prefix) > 0 && prefix[len(prefix)-1] == '/' {
+		prefix = prefix[:len(prefix)-1]
+	}
+
 	samplesComparator := querytee.NewSamplesComparator()
 	return []querytee.Route{
-		{Path: "/api/v1/query", RouteName: "api_v1_query", Methods: []string{"GET"}, ResponseComparator: samplesComparator},
-		{Path: "/api/v1/query_range", RouteName: "api_v1_query_range", Methods: []string{"GET"}, ResponseComparator: samplesComparator},
-		{Path: "/api/v1/labels", RouteName: "api_v1_labels", Methods: []string{"GET"}, ResponseComparator: nil},
-		{Path: "/api/v1/label/{name}/values", RouteName: "api_v1_label_name_values", Methods: []string{"GET"}, ResponseComparator: nil},
-		{Path: "/api/v1/series", RouteName: "api_v1_series", Methods: []string{"GET"}, ResponseComparator: nil},
-		{Path: "/api/v1/metadata", RouteName: "api_v1_metadata", Methods: []string{"GET"}, ResponseComparator: nil},
-		{Path: "/api/v1/rules", RouteName: "api_v1_rules", Methods: []string{"GET"}, ResponseComparator: nil},
-		{Path: "/api/v1/alerts", RouteName: "api_v1_alerts", Methods: []string{"GET"}, ResponseComparator: nil},
+		{Path: prefix + "/api/v1/query", RouteName: "api_v1_query", Methods: []string{"GET"}, ResponseComparator: samplesComparator},
+		{Path: prefix + "/api/v1/query_range", RouteName: "api_v1_query_range", Methods: []string{"GET"}, ResponseComparator: samplesComparator},
+		{Path: prefix + "/api/v1/labels", RouteName: "api_v1_labels", Methods: []string{"GET"}, ResponseComparator: nil},
+		{Path: prefix + "/api/v1/label/{name}/values", RouteName: "api_v1_label_name_values", Methods: []string{"GET"}, ResponseComparator: nil},
+		{Path: prefix + "/api/v1/series", RouteName: "api_v1_series", Methods: []string{"GET"}, ResponseComparator: nil},
+		{Path: prefix + "/api/v1/metadata", RouteName: "api_v1_metadata", Methods: []string{"GET"}, ResponseComparator: nil},
+		{Path: prefix + "/api/v1/rules", RouteName: "api_v1_rules", Methods: []string{"GET"}, ResponseComparator: nil},
+		{Path: prefix + "/api/v1/alerts", RouteName: "api_v1_alerts", Methods: []string{"GET"}, ResponseComparator: nil},
 	}
 }
diff --git a/cmd/query-tee/main_test.go b/cmd/query-tee/main_test.go
new file mode 100644
index 00000000000..48c5347e694
--- /dev/null
+++ b/cmd/query-tee/main_test.go
@@ -0,0 +1,20 @@
+package main
+
+import (
+	"strings"
+	"testing"
+
+	"github.com/stretchr/testify/assert"
+)
+
+func TestCortexReadRoutes(t *testing.T) {
+	routes := cortexReadRoutes("")
+	for _, r := range routes {
+		assert.True(t, strings.HasPrefix(r.Path, "/api/v1/"))
+	}
+
+	routes = cortexReadRoutes("/some/random/prefix///")
+	for _, r := range routes {
+		assert.True(t, strings.HasPrefix(r.Path, "/some/random/prefix/api/v1/"))
+	}
+}