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Size of localization in image rendering #17

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destelisa opened this issue Jun 7, 2024 · 1 comment
Open

Size of localization in image rendering #17

destelisa opened this issue Jun 7, 2024 · 1 comment
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enhancement New feature or request
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@destelisa
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It would be helful being able to set size of the radius when rendering the localization at least for the final filtered image. This could be done either based on the calculated localization precision of the image, arbitrarly, or each localization rendered based on its own precision.
For publications, we saw that rendering the images with e.g. 2nm is not feasible (you do not see anything!) and increasing the size to e.g. 20nm is necessary.
Alternatives, are to export the table of localizations generated by PyMINFLUX and use other software for the rendering
Thanks!

@destelisa destelisa added the enhancement New feature or request label Jun 7, 2024
@aarpon
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aarpon commented Jun 7, 2024

Hi, @destelisa, thanks for the suggestion. We have already planned to work on this, and will most likely add it to the upcoming release. In the meanwhile, I would suggest a few workarounds:

  • In File > Options (Preferences on macOS), reduce the value of Resolution (DPI) for exporting plots: this should make the size of the dots larger (particularly on macOS, the default might indeed be a bit aggressive);
  • Save the filtered data to a .pmx file (File > Save) and then import the .pmxfile in ParaView, where you will have more flexibility in setting up your 3D renderer. Please follow this instructions to set up the plug-in: https://github.com/bsse-scf/pyMINFLUX/wiki/pyMINFLUX-user-manual#3d-data-viewer
  • Export the filtered data to a .csvfile (File > Export data) and import it into a tool of your choice. If you are familiar with Python and the Matplotlib library, you can create publication-quality figures with a few lines of code.

@aarpon aarpon self-assigned this Jun 7, 2024
@aarpon aarpon added this to the 0.5.0 milestone Jun 7, 2024
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