diff --git a/Box.V2.Test/BoxConfigTest.cs b/Box.V2.Test/BoxConfigTest.cs
index 66141bf79..fd5ed8bb6 100644
--- a/Box.V2.Test/BoxConfigTest.cs
+++ b/Box.V2.Test/BoxConfigTest.cs
@@ -85,5 +85,30 @@ public void BoxConfig_SetBoxAccountApiHostUri()
             Assert.AreEqual(newConfig.BoxAccountApiHostUri.ToString(), exampleUri + "/");
             Assert.AreEqual(newConfig.AuthCodeBaseUri.ToString(), exampleUri + "/" + "oauth2/authorize");
         }
+
+        [TestMethod]
+        public void BoxConfig_DefaultJWTAudience()
+        {
+            var exampleUri = new Uri("https://example.com/account");
+            var newConfig = new BoxConfigBuilder("", "")
+                .SetBoxApiHostUri(exampleUri)
+                .Build();
+
+            Assert.AreEqual(newConfig.JWTAudience, "https://api.box.com/oauth2/token");
+        }
+
+        [TestMethod]
+        public void BoxConfig_SetJWTAudience()
+        {
+            var exampleUri = new Uri("https://example.com/account");
+            var customAudience = "custom_audience/oauth2/token";
+            var newConfig = new BoxConfigBuilder("", "")
+                .SetBoxApiHostUri(exampleUri)
+                .SetJWTAudience(customAudience)
+                .Build();
+
+            Assert.AreEqual(newConfig.BoxApiHostUri.ToString(), exampleUri + "/");
+            Assert.AreEqual(newConfig.JWTAudience, customAudience);
+        }
     }
 }
diff --git a/Box.V2/Config/BoxConfig.cs b/Box.V2/Config/BoxConfig.cs
index 26695f174..be7333072 100644
--- a/Box.V2/Config/BoxConfig.cs
+++ b/Box.V2/Config/BoxConfig.cs
@@ -66,6 +66,7 @@ public BoxConfig(BoxConfigBuilder builder)
             WebProxy = builder.WebProxy;
             Timeout = builder.Timeout;
             RetryStrategy = builder.RetryStrategy;
+            JWTAudience = builder.JWTAudience;
         }
 
         /// <summary>
@@ -155,6 +156,17 @@ public Uri BoxApiUri
             private set { _boxApiUri = value; }
         }
 
+        private string _jwtAudience;
+
+        /// <summary>
+        /// Audience claim for JWT token. 
+        /// </summary>
+        public string JWTAudience
+        {
+            get { return _jwtAudience ?? Constants.BoxAuthTokenApiUriString; }
+            private set { _jwtAudience = value; }
+        }
+
         public string ClientId { get; private set; }
         public string ConsumerKey { get; private set; }
         public string ClientSecret { get; private set; }
diff --git a/Box.V2/Config/BoxConfigBuilder.cs b/Box.V2/Config/BoxConfigBuilder.cs
index 9d4863904..2188c135d 100644
--- a/Box.V2/Config/BoxConfigBuilder.cs
+++ b/Box.V2/Config/BoxConfigBuilder.cs
@@ -239,6 +239,17 @@ public BoxConfigBuilder SetRetryStrategy(IRetryStrategy retryStrategy)
             return this;
         }
 
+        /// <summary>
+        /// Sets audience claim used in JWT tokens.
+        /// </summary>
+        /// <param name="jwtAudience">Audience claim value</param>
+        /// <returns>this BoxConfigBuilder object for chaining</returns>
+        public BoxConfigBuilder SetJWTAudience(string jwtAudience)
+        {
+            _jwtAudience = jwtAudience;
+            return this;
+        }
+
         public string ClientId { get; private set; }
         public string ClientSecret { get; private set; }
         public string EnterpriseId { get; private set; }
@@ -247,7 +258,6 @@ public BoxConfigBuilder SetRetryStrategy(IRetryStrategy retryStrategy)
         public string JWTPublicKeyId { get; private set; }
         public string UserAgent { get; private set; }
 
-
         public Uri BoxApiHostUri { get; private set; } = new Uri(Constants.BoxApiHostUriString);
         public Uri BoxAccountApiHostUri { get; private set; } = new Uri(Constants.BoxAccountApiHostUriString);
         public Uri BoxUploadApiUri { get; private set; } = new Uri(new Uri(Constants.BoxUploadApiUriWithoutVersionString), Constants.BoxApiCurrentVersionUriString);
@@ -283,6 +293,17 @@ public Uri BoxApiUri
         /// </summary>
         public IRetryStrategy RetryStrategy { get; private set; } = new ExponentialBackoff();
 
+        private string _jwtAudience;
+
+        /// <summary>
+        /// Audience claim for JWT token. 
+        /// </summary>
+        public string JWTAudience
+        {
+            get { return _jwtAudience ?? Constants.BoxAuthTokenApiUriString; }
+            private set { _jwtAudience = value; }
+        }
+
         private Uri EnsureEndsWithSlash(Uri uri)
         {
             return uri.ToString().EndsWith("/") ? uri : new Uri($"{uri}{"/"}");
diff --git a/Box.V2/Config/IBoxConfig.cs b/Box.V2/Config/IBoxConfig.cs
index 821454d3e..a5f4740c9 100644
--- a/Box.V2/Config/IBoxConfig.cs
+++ b/Box.V2/Config/IBoxConfig.cs
@@ -146,5 +146,10 @@ public interface IBoxConfig
         /// Retry strategy for failed requests
         /// </summary>
         IRetryStrategy RetryStrategy { get; }
+        /// <summary>
+        /// JWT token audience claim.
+        /// https://api.box.com/oauth2/token is used by default.
+        /// </summary>
+        string JWTAudience { get; }
     }
 }
diff --git a/Box.V2/JWTAuth/BoxJWTAuth.cs b/Box.V2/JWTAuth/BoxJWTAuth.cs
index 0c1309824..57dae1ca2 100644
--- a/Box.V2/JWTAuth/BoxJWTAuth.cs
+++ b/Box.V2/JWTAuth/BoxJWTAuth.cs
@@ -265,7 +265,7 @@ private string ConstructJWTAssertion(string sub, string boxSubType, DateTimeOffs
                 expireTime = nowOverride.Value.AddSeconds(30);
             }
 
-            var payload = new JwtPayload(_boxConfig.ClientId, new Uri(Constants.BoxAuthTokenApiUriString).ToString(),
+            var payload = new JwtPayload(_boxConfig.ClientId, _boxConfig.JWTAudience,
                 claims, null, expireTime.LocalDateTime);
 
             var header = new JwtHeader(signingCredentials: _credentials);