diff --git a/crates/uv-python/src/interpreter.rs b/crates/uv-python/src/interpreter.rs
index b2c48a1900f..b3bde5187b1 100644
--- a/crates/uv-python/src/interpreter.rs
+++ b/crates/uv-python/src/interpreter.rs
@@ -15,7 +15,7 @@ use serde::{Deserialize, Serialize};
 use thiserror::Error;
 use tracing::{debug, trace, warn};
 
-use uv_cache::{Cache, CacheBucket, CachedByTimestamp, Freshness};
+use uv_cache::{Cache, CacheBucket, CacheEntry, CachedByTimestamp, Freshness};
 use uv_cache_info::Timestamp;
 use uv_cache_key::cache_digest;
 use uv_fs::{
@@ -69,7 +69,8 @@ pub struct Interpreter {
 impl Interpreter {
     /// Detect the interpreter info for the given Python executable.
     pub fn query(executable: impl AsRef<Path>, cache: &Cache) -> Result<Self, Error> {
-        let info = InterpreterInfo::query_cached(executable.as_ref(), cache)?;
+        let executable = executable.as_ref();
+        let info = InterpreterInfo::query_cached(executable, cache)?;
 
         debug_assert!(
             info.sys_executable.is_absolute(),
@@ -97,10 +98,23 @@ impl Interpreter {
             tags: OnceLock::new(),
             target: None,
             prefix: None,
-            real_executable: executable.as_ref().to_path_buf(),
+            real_executable: executable.to_path_buf(),
         })
     }
 
+    /// Remove any cached metadata for the given Python executable.
+    pub fn clear_cache(executable: impl AsRef<Path>, cache: &Cache) -> Result<(), Error> {
+        let absolute = std::path::absolute(executable.as_ref())?;
+        let canonical = canonicalize_executable(&absolute)?;
+        let cache_entry = InterpreterInfo::cache_entry(&absolute, &canonical, cache);
+
+        match fs::remove_file(cache_entry.path()) {
+            Ok(()) => Ok(()),
+            Err(err) if err.kind() == io::ErrorKind::NotFound => Ok(()),
+            Err(err) => Err(err.into()),
+        }
+    }
+
     /// Return a new [`Interpreter`] with the given virtual environment root.
     #[must_use]
     pub fn with_virtualenv(self, virtualenv: VirtualEnvironment) -> Self {
@@ -1117,6 +1131,41 @@ impl InterpreterInfo {
         Ok(())
     }
 
+    /// Return the cache entry for an interpreter's absolute and canonical executable paths.
+    fn cache_entry(absolute: &Path, canonical: &Path, cache: &Cache) -> CacheEntry {
+        let python_executable = env::var_os(EnvVars::PYTHONEXECUTABLE).map(PathBuf::from);
+        let pyvenv_launcher = env::var_os(EnvVars::PYVENV_LAUNCHER).map(PathBuf::from);
+
+        cache.entry(
+            CacheBucket::Interpreter,
+            // Shard interpreter metadata by host architecture, operating system, and version, to
+            // invalidate the cache (e.g.) on OS upgrades.
+            cache_digest(&(
+                ARCH,
+                uv_platform::OsType::from_env()
+                    .map(|os_type| os_type.to_string())
+                    .unwrap_or_default(),
+                uv_platform::OsRelease::from_env()
+                    .map(|os_release| os_release.to_string())
+                    .unwrap_or_default(),
+            )),
+            // We use the absolute path for the cache entry to avoid cache collisions for relative
+            // paths. But we don't want to query the executable with symbolic links resolved because
+            // that can change reported values, e.g., `sys.executable`. We include the canonical
+            // path in the cache entry as well, otherwise we can have cache collisions if an
+            // absolute path refers to different interpreters with matching ctimes, e.g., if you
+            // have a `.venv/bin/python` pointing to both Python 3.12 and Python 3.13 that were
+            // modified at the same time.
+            //
+            // Launcher overrides can also change the reported executable and virtual environment
+            // without changing either executable path.
+            format!(
+                "{}.msgpack",
+                cache_digest(&(absolute, canonical, &python_executable, &pyvenv_launcher))
+            ),
+        )
+    }
+
     /// A wrapper around [`markers::query_interpreter_info`] to cache the computed markers.
     ///
     /// Running a Python script is (relatively) expensive, and the markers won't change
@@ -1150,37 +1199,7 @@ impl InterpreterInfo {
         };
 
         let canonical = canonicalize_executable(&absolute).map_err(handle_io_error)?;
-        let python_executable = env::var_os(EnvVars::PYTHONEXECUTABLE).map(PathBuf::from);
-        let pyvenv_launcher = env::var_os(EnvVars::PYVENV_LAUNCHER).map(PathBuf::from);
-
-        let cache_entry = cache.entry(
-            CacheBucket::Interpreter,
-            // Shard interpreter metadata by host architecture, operating system, and version, to
-            // invalidate the cache (e.g.) on OS upgrades.
-            cache_digest(&(
-                ARCH,
-                uv_platform::OsType::from_env()
-                    .map(|os_type| os_type.to_string())
-                    .unwrap_or_default(),
-                uv_platform::OsRelease::from_env()
-                    .map(|os_release| os_release.to_string())
-                    .unwrap_or_default(),
-            )),
-            // We use the absolute path for the cache entry to avoid cache collisions for relative
-            // paths. But we don't want to query the executable with symbolic links resolved because
-            // that can change reported values, e.g., `sys.executable`. We include the canonical
-            // path in the cache entry as well, otherwise we can have cache collisions if an
-            // absolute path refers to different interpreters with matching ctimes, e.g., if you
-            // have a `.venv/bin/python` pointing to both Python 3.12 and Python 3.13 that were
-            // modified at the same time.
-            //
-            // Launcher overrides can also change the reported executable and virtual environment
-            // without changing either executable path.
-            format!(
-                "{}.msgpack",
-                cache_digest(&(&absolute, &canonical, &python_executable, &pyvenv_launcher))
-            ),
-        );
+        let cache_entry = Self::cache_entry(&absolute, &canonical, cache);
 
         // We check the timestamp of the canonicalized executable to check if an underlying
         // interpreter has been modified.
@@ -1342,8 +1361,10 @@ fn python_home(interpreter: &Path) -> Option<PathBuf> {
 mod tests {
     use std::str::FromStr;
 
+    use anyhow::Result;
     use fs_err as fs;
     use indoc::{formatdoc, indoc};
+    use serde_json::Value;
     use tempfile::tempdir;
 
     use uv_cache::{Cache, CacheBucket};
@@ -1352,12 +1373,8 @@ mod tests {
 
     use crate::Interpreter;
 
-    #[tokio::test]
-    async fn test_cache_invalidation() {
-        let mock_dir = tempdir().unwrap();
-        let mocked_interpreter = mock_dir.path().join("python");
-        let query_log = mock_dir.path().join("queries");
-        let json = indoc! {r##"
+    fn mocked_interpreter_response() -> &'static str {
+        indoc! {r##"
         {
             "result": "success",
             "platform": {
@@ -1415,7 +1432,14 @@ mod tests {
             "debug_enabled": false
         }
     "##}
-        .replace(
+    }
+
+    #[tokio::test]
+    async fn test_cache_invalidation() {
+        let mock_dir = tempdir().unwrap();
+        let mocked_interpreter = mock_dir.path().join("python");
+        let query_log = mock_dir.path().join("queries");
+        let json = mocked_interpreter_response().replace(
             "{sys_executable}",
             &mocked_interpreter.display().to_string(),
         );
@@ -1476,4 +1500,71 @@ mod tests {
             "queried\nqueried\n"
         );
     }
+
+    #[tokio::test]
+    async fn test_cache_eviction_with_unchanged_executable() -> Result<()> {
+        let mock_dir = tempdir()?;
+        let mocked_interpreter = mock_dir.path().join("python");
+        let response_file = mock_dir.path().join("response.json");
+        let query_count = mock_dir.path().join("queries");
+
+        let mut response = serde_json::from_str::<Value>(mocked_interpreter_response())?;
+        response["sys_executable"] = serde_json::to_value(&mocked_interpreter)?;
+        fs::write(&response_file, serde_json::to_vec(&response)?)?;
+        fs::write(
+            &mocked_interpreter,
+            formatdoc! {r#"
+                #!/bin/sh
+                printf '.' >> "{}"
+                cat "{}"
+            "#, query_count.display(), response_file.display()},
+        )?;
+        fs::set_permissions(
+            &mocked_interpreter,
+            std::os::unix::fs::PermissionsExt::from_mode(0o770),
+        )?;
+
+        let cache = Cache::temp()?.init().await?;
+        let original_version = Version::from_str("3.12.0")?;
+        let updated_version = Version::from_str("3.12.13")?;
+
+        assert_eq!(
+            Interpreter::query(&mocked_interpreter, &cache)?.python_version(),
+            &original_version
+        );
+
+        response["markers"]["implementation_version"] = "3.12.13".into();
+        response["markers"]["python_full_version"] = "3.12.13".into();
+        fs::write(&response_file, serde_json::to_vec(&response)?)?;
+
+        assert_eq!(
+            Interpreter::query(&mocked_interpreter, &cache)?.python_version(),
+            &original_version,
+            "an unchanged executable should retain its cached interpreter metadata"
+        );
+
+        Interpreter::clear_cache(&mocked_interpreter, &cache)?;
+        assert_eq!(
+            fs::read_to_string(&query_count)?,
+            ".",
+            "clearing cached metadata should not query the interpreter"
+        );
+        assert_eq!(
+            Interpreter::query(&mocked_interpreter, &cache)?.python_version(),
+            &updated_version,
+            "clearing the cache should force the next query to run the interpreter"
+        );
+        assert_eq!(
+            Interpreter::query(&mocked_interpreter, &cache)?.python_version(),
+            &updated_version,
+            "the next query should persist the updated interpreter metadata"
+        );
+        assert_eq!(
+            fs::read_to_string(&query_count)?,
+            "..",
+            "the updated interpreter metadata should be cached again"
+        );
+
+        Ok(())
+    }
 }
diff --git a/crates/uv/src/commands/project/mod.rs b/crates/uv/src/commands/project/mod.rs
index f03b7ddb404..d2f0ebce95b 100644
--- a/crates/uv/src/commands/project/mod.rs
+++ b/crates/uv/src/commands/project/mod.rs
@@ -1138,14 +1138,35 @@ fn discover_project_environment(
         return Ok(None);
     };
 
-    match check_environment_compatibility(
+    let compatibility = check_environment_compatibility(
         &environment,
         EnvironmentKind::Project,
         python_request,
         python_preference,
         requires_python,
         cache,
-    ) {
+    );
+
+    // Conflicting versions for the same base interpreter indicate its cached metadata may be
+    // corrupted. Clear the entry before interpreter discovery can select stale metadata.
+    if matches!(
+        &compatibility,
+        Err(EnvironmentIncompatibilityError::PyenvVersionConflict(..))
+    ) && let Ok(base_executable) = environment.interpreter().to_base_python()
+        && let Ok(base_interpreter) = Interpreter::query(&base_executable, cache)
+        && environment.uses(&base_interpreter)
+        && environment.interpreter().python_version() != base_interpreter.python_version()
+    {
+        debug!(
+            "Clearing cached interpreter info for {} after finding conflicting Python versions ({} and {})",
+            base_executable.user_display(),
+            base_interpreter.python_version(),
+            environment.interpreter().python_version(),
+        );
+        Interpreter::clear_cache(&base_executable, cache)?;
+    }
+
+    match compatibility {
         Ok(()) => Ok(Some(environment)),
         Err(err) if matches!(policy, ProjectEnvironmentPolicy::Preserve) => {
             if centralized {