diff --git a/crates/uv-configuration/src/target_triple.rs b/crates/uv-configuration/src/target_triple.rs
index 9adbd27d87b..d923a03d4d3 100644
--- a/crates/uv-configuration/src/target_triple.rs
+++ b/crates/uv-configuration/src/target_triple.rs
@@ -359,9 +359,15 @@ pub enum TargetTriple {
     X8664LinuxAndroid,
 
     /// A wasm32 target using the Pyodide 2024 platform. Meant for use with Python 3.12.
+    /// See <https://pyodide.org/en/stable/development/abi/312.html>
     #[cfg_attr(feature = "clap", value(name = "wasm32-pyodide2024"))]
     Wasm32Pyodide2024,
 
+    /// A wasm32 target using the Pyodide 2025 platform. Meant for use with Python 3.13.
+    /// See <https://pyodide.org/en/stable/development/abi/313.html>
+    #[cfg_attr(feature = "clap", value(name = "wasm32-pyodide2025"))]
+    Wasm32Pyodide2025,
+
     /// An ARM64 target for iOS device
     ///
     /// By default, iOS 13.0 is used, but respects the `IPHONEOS_DEPLOYMENT_TARGET`
@@ -624,6 +630,13 @@ impl TargetTriple {
                 },
                 Arch::Wasm32,
             ),
+            Self::Wasm32Pyodide2025 => Platform::new(
+                Os::Pyodide {
+                    major: 2025,
+                    minor: 0,
+                },
+                Arch::Wasm32,
+            ),
             Self::Aarch64LinuxAndroid => {
                 let api_level = android_api_level().map_or(24, |api_level| {
                     debug!("Found Android API level: {}", api_level);
@@ -725,6 +738,7 @@ impl TargetTriple {
             Self::Aarch64LinuxAndroid => "aarch64",
             Self::X8664LinuxAndroid => "x86_64",
             Self::Wasm32Pyodide2024 => "wasm32",
+            Self::Wasm32Pyodide2025 => "wasm32",
             Self::Arm64Ios => "arm64",
             Self::Arm64IosSimulator => "arm64",
             Self::X8664IosSimulator => "x86_64",
@@ -773,6 +787,7 @@ impl TargetTriple {
             Self::Aarch64LinuxAndroid => "Android",
             Self::X8664LinuxAndroid => "Android",
             Self::Wasm32Pyodide2024 => "Emscripten",
+            Self::Wasm32Pyodide2025 => "Emscripten",
             Self::Arm64Ios => "iOS",
             Self::Arm64IosSimulator => "iOS",
             Self::X8664IosSimulator => "iOS",
@@ -824,6 +839,7 @@ impl TargetTriple {
             // https://github.com/emscripten-core/emscripten/blob/4.0.8/system/lib/libc/emscripten_syscall_stubs.c#L63
             // It doesn't really seem to mean anything? But for completeness we include it here.
             Self::Wasm32Pyodide2024 => "#1",
+            Self::Wasm32Pyodide2025 => "#1",
             Self::Arm64Ios => "",
             Self::Arm64IosSimulator => "",
             Self::X8664IosSimulator => "",
@@ -872,8 +888,10 @@ impl TargetTriple {
             Self::Aarch64LinuxAndroid => "",
             Self::X8664LinuxAndroid => "",
             // This is the Emscripten compiler version for Pyodide 2024.
-            // See https://pyodide.org/en/stable/development/abi.html#pyodide-2024-0
+            // See https://pyodide.org/en/stable/development/abi/312.html
             Self::Wasm32Pyodide2024 => "3.1.58",
+            // See https://pyodide.org/en/stable/development/abi/313.html
+            Self::Wasm32Pyodide2025 => "4.0.9",
             Self::Arm64Ios => "",
             Self::Arm64IosSimulator => "",
             Self::X8664IosSimulator => "",
@@ -922,6 +940,7 @@ impl TargetTriple {
             Self::Aarch64LinuxAndroid => "posix",
             Self::X8664LinuxAndroid => "posix",
             Self::Wasm32Pyodide2024 => "posix",
+            Self::Wasm32Pyodide2025 => "posix",
             Self::Arm64Ios => "posix",
             Self::Arm64IosSimulator => "posix",
             Self::X8664IosSimulator => "posix",
@@ -970,6 +989,7 @@ impl TargetTriple {
             Self::Aarch64LinuxAndroid => "android",
             Self::X8664LinuxAndroid => "android",
             Self::Wasm32Pyodide2024 => "emscripten",
+            Self::Wasm32Pyodide2025 => "emscripten",
             Self::Arm64Ios => "ios",
             Self::Arm64IosSimulator => "ios",
             Self::X8664IosSimulator => "ios",
@@ -1018,6 +1038,7 @@ impl TargetTriple {
             Self::Aarch64LinuxAndroid => false,
             Self::X8664LinuxAndroid => false,
             Self::Wasm32Pyodide2024 => false,
+            Self::Wasm32Pyodide2025 => false,
             Self::Arm64Ios => false,
             Self::Arm64IosSimulator => false,
             Self::X8664IosSimulator => false,