diff --git a/.github/workflows/sync-python-releases.yml b/.github/workflows/sync-python-releases.yml
index bc54925ccda5..dbe92e918749 100644
--- a/.github/workflows/sync-python-releases.yml
+++ b/.github/workflows/sync-python-releases.yml
@@ -20,8 +20,8 @@ jobs:
       - uses: hynek/setup-cached-uv@v1
       - name: Sync Python Releases
         run: |
-          uv run --isolated -- fetch-download-metadata.py
-          uv run --isolated -- template-download-metadata.py
+          uv run --no-workspace -- fetch-download-metadata.py
+          uv run --no-workspace -- template-download-metadata.py
         working-directory: ./crates/uv-python
         env:
           GITHUB_TOKEN: ${{ secrets.GITHUB_TOKEN }}
diff --git a/crates/uv-cli/src/lib.rs b/crates/uv-cli/src/lib.rs
index 4faa6cf3492d..40bf4d3f2ea5 100644
--- a/crates/uv-cli/src/lib.rs
+++ b/crates/uv-cli/src/lib.rs
@@ -154,7 +154,7 @@ pub struct GlobalArgs {
 
     /// Avoid discovering a `pyproject.toml` or `uv.toml` file in the current directory or any
     /// parent directories.
-    #[arg(global = true, long)]
+    #[arg(global = true, long, hide = true)]
     pub isolated: bool,
 
     /// Show the resolved settings for the current command.
diff --git a/crates/uv-python/fetch-download-metadata.py b/crates/uv-python/fetch-download-metadata.py
index 1715a967e807..dd532f07e34e 100755
--- a/crates/uv-python/fetch-download-metadata.py
+++ b/crates/uv-python/fetch-download-metadata.py
@@ -11,7 +11,7 @@
 
 Usage:
 
-    uv run --isolated -- crates/uv-python/fetch-download-metadata.py
+    uv run --no-workspace -- crates/uv-python/fetch-download-metadata.py
 
 Acknowledgements:
 
diff --git a/crates/uv-python/template-download-metadata.py b/crates/uv-python/template-download-metadata.py
index 6f21855a1578..1c8c4e3d4833 100755
--- a/crates/uv-python/template-download-metadata.py
+++ b/crates/uv-python/template-download-metadata.py
@@ -11,7 +11,7 @@
 
 Usage:
 
-    uv run --isolated -- crates/uv-python/template-download-metadata.py
+    uv run --no-workspace -- crates/uv-python/template-download-metadata.py
 """
 
 import argparse
diff --git a/crates/uv/src/lib.rs b/crates/uv/src/lib.rs
index 45d949582cef..fc9c4a207546 100644
--- a/crates/uv/src/lib.rs
+++ b/crates/uv/src/lib.rs
@@ -23,6 +23,7 @@ use uv_cli::{SelfCommand, SelfNamespace};
 use uv_configuration::Concurrency;
 use uv_requirements::RequirementsSource;
 use uv_settings::{Combine, FilesystemOptions};
+use uv_warnings::warn_user;
 use uv_workspace::{DiscoveryOptions, Workspace};
 
 use crate::commands::{ExitStatus, ToolRunCommand};
@@ -136,6 +137,14 @@ async fn run(cli: Cli) -> Result<ExitStatus> {
         )
     }))?;
 
+    if globals.isolated {
+        if globals.preview.is_enabled() {
+            warn_user!("The `--isolated` flag is deprecated. Instead, use `--no-config` to prevent uv from discovering configuration files, or `--no-workspace` to prevent uv from discovering a workspace in `uv run` and other commands.");
+        } else {
+            warn_user!("The `--isolated` flag is deprecated. Instead, use `--no-config` to prevent uv from discovering configuration files.");
+        }
+    }
+
     debug!("uv {}", version::version());
 
     // Write out any resolved settings.
diff --git a/crates/uv/tests/help.rs b/crates/uv/tests/help.rs
index ae95fa5834f4..3178c148dd5a 100644
--- a/crates/uv/tests/help.rs
+++ b/crates/uv/tests/help.rs
@@ -43,9 +43,6 @@ fn help() {
           --python-fetch <PYTHON_FETCH>
               Whether to automatically download Python when required [possible values: automatic,
               manual]
-          --isolated
-              Avoid discovering a `pyproject.toml` or `uv.toml` file in the current directory or any
-              parent directories
           --no-progress
               Hides all progress outputs when set
       -n, --no-cache
@@ -109,9 +106,6 @@ fn help_flag() {
           --python-fetch <PYTHON_FETCH>
               Whether to automatically download Python when required [possible values: automatic,
               manual]
-          --isolated
-              Avoid discovering a `pyproject.toml` or `uv.toml` file in the current directory or any
-              parent directories
           --no-progress
               Hides all progress outputs when set
       -n, --no-cache
@@ -174,9 +168,6 @@ fn help_short_flag() {
           --python-fetch <PYTHON_FETCH>
               Whether to automatically download Python when required [possible values: automatic,
               manual]
-          --isolated
-              Avoid discovering a `pyproject.toml` or `uv.toml` file in the current directory or any
-              parent directories
           --no-progress
               Hides all progress outputs when set
       -n, --no-cache
@@ -280,10 +271,6 @@ fn help_subcommand() {
               - manual:    Do not automatically fetch managed Python installations; require explicit
                 installation
 
-          --isolated
-              Avoid discovering a `pyproject.toml` or `uv.toml` file in the current directory or any
-              parent directories
-
           --no-progress
               Hides all progress outputs when set
 
@@ -408,10 +395,6 @@ fn help_subsubcommand() {
               - manual:    Do not automatically fetch managed Python installations; require explicit
                 installation
 
-          --isolated
-              Avoid discovering a `pyproject.toml` or `uv.toml` file in the current directory or any
-              parent directories
-
           --no-progress
               Hides all progress outputs when set
 
@@ -490,9 +473,6 @@ fn help_flag_subcommand() {
           --python-fetch <PYTHON_FETCH>
               Whether to automatically download Python when required [possible values: automatic,
               manual]
-          --isolated
-              Avoid discovering a `pyproject.toml` or `uv.toml` file in the current directory or any
-              parent directories
           --no-progress
               Hides all progress outputs when set
       -n, --no-cache
@@ -552,9 +532,6 @@ fn help_flag_subsubcommand() {
           --python-fetch <PYTHON_FETCH>
               Whether to automatically download Python when required [possible values: automatic,
               manual]
-          --isolated
-              Avoid discovering a `pyproject.toml` or `uv.toml` file in the current directory or any
-              parent directories
           --no-progress
               Hides all progress outputs when set
       -n, --no-cache
@@ -671,9 +648,6 @@ fn help_with_global_option() {
           --python-fetch <PYTHON_FETCH>
               Whether to automatically download Python when required [possible values: automatic,
               manual]
-          --isolated
-              Avoid discovering a `pyproject.toml` or `uv.toml` file in the current directory or any
-              parent directories
           --no-progress
               Hides all progress outputs when set
       -n, --no-cache
@@ -770,9 +744,6 @@ fn test_with_no_pager() {
           --python-fetch <PYTHON_FETCH>
               Whether to automatically download Python when required [possible values: automatic,
               manual]
-          --isolated
-              Avoid discovering a `pyproject.toml` or `uv.toml` file in the current directory or any
-              parent directories
           --no-progress
               Hides all progress outputs when set
       -n, --no-cache
diff --git a/crates/uv/tests/init.rs b/crates/uv/tests/init.rs
index 55ebd47308bc..3338c1f9db3f 100644
--- a/crates/uv/tests/init.rs
+++ b/crates/uv/tests/init.rs
@@ -580,6 +580,7 @@ fn init_isolated() -> Result<()> {
     ----- stdout -----
 
     ----- stderr -----
+    warning: The `--isolated` flag is deprecated. Instead, use `--no-config` to prevent uv from discovering configuration files.
     warning: `uv init` is experimental and may change without warning
     Initialized project `foo`
     "###);
diff --git a/scripts/release.sh b/scripts/release.sh
index f5ba61fb6d57..de3ff0b916b4 100755
--- a/scripts/release.sh
+++ b/scripts/release.sh
@@ -11,14 +11,14 @@ echo "Updating metadata with rooster..."
 cd "$project_root"
 
 # Update the preview changelog
-uv tool run --from 'rooster-blue>=0.0.7' --python 3.12 --isolated -- \
+uv tool run --from 'rooster-blue>=0.0.7' --python 3.12 --isolate -- \
     rooster release "$@" \
     --only-sections preview \
     --changelog-file PREVIEW-CHANGELOG.md \
     --no-update-pyproject --no-update-version-files
 
 # Update the real changelog
-uv tool run --from 'rooster-blue>=0.0.7' --python 3.12 --isolated -- \
+uv tool run --from 'rooster-blue>=0.0.7' --python 3.12 --isolate -- \
     rooster release "$@" --without-sections preview
 
 echo "Updating lockfile..."