diff --git a/crates/ty_python_semantic/resources/mdtest/typed_dict.md b/crates/ty_python_semantic/resources/mdtest/typed_dict.md
index 4b6253afc1258..ae9e2480eeeb7 100644
--- a/crates/ty_python_semantic/resources/mdtest/typed_dict.md
+++ b/crates/ty_python_semantic/resources/mdtest/typed_dict.md
@@ -2373,6 +2373,23 @@ partial_no_year = PartialWithRequired(name="The Matrix")
 reveal_type(partial_no_year)  # revealed: PartialWithRequired
 ```
 
+## Function syntax with invalid qualifiers
+
+All type qualifiers except for `ReadOnly`, `Required` and `NotRequired` are rejected:
+
+```py
+from typing_extensions import ClassVar, Final, TypedDict
+from dataclasses import InitVar
+
+TD1 = TypedDict("TD1", {"x": ClassVar[int]})  # error: [invalid-type-form]
+TD2 = TypedDict("TD2", {"x": Final[int]})  # error: [invalid-type-form]
+TD3 = TypedDict("TD3", {"x": InitVar[int]})  # error: [invalid-type-form]
+
+class TD4(TypedDict("TD4", {"x": ClassVar[int]})): ...  # error: [invalid-type-form]
+class TD5(TypedDict("TD5", {"x": Final[int]})): ...  # error: [invalid-type-form]
+class TD6(TypedDict("TD6", {"x": InitVar[int]})): ...  # error: [invalid-type-form]
+```
+
 ## Function syntax with `closed`
 
 The `closed` keyword is accepted but not yet fully supported:
@@ -2398,7 +2415,8 @@ def f(closed: bool) -> None:
 The `extra_items` keyword is accepted and validated as an annotation expression:
 
 ```py
-from typing_extensions import ReadOnly, TypedDict
+from typing_extensions import ReadOnly, TypedDict, NotRequired, Required, ClassVar, Final
+from dataclasses import InitVar
 
 # extra_items is accepted (no error)
 MovieWithExtras = TypedDict("MovieWithExtras", {"name": str}, extra_items=bool)
@@ -2415,10 +2433,24 @@ class Foo(TypedDict("T", {}, extra_items="Foo | None")): ...
 
 reveal_type(Foo)  # revealed: <class 'Foo'>
 
-# Type qualifiers like ReadOnly are valid in extra_items (annotation expression, not type expression):
+# The `ReadOnly` type qualifier is valid in `extra_items` (annotation expression, not type expression):
 TD2 = TypedDict("TD2", {}, extra_items=ReadOnly[int])
 
 class Bar(TypedDict("TD3", {}, extra_items=ReadOnly[int])): ...
+
+# But all other qualifiers are rejected:
+
+TD4 = TypedDict("TD4", {}, extra_items=Required[int])  # error: [invalid-type-form]
+TD5 = TypedDict("TD5", {}, extra_items=NotRequired[int])  # error: [invalid-type-form]
+TD6 = TypedDict("TD6", {}, extra_items=ClassVar[int])  # error: [invalid-type-form]
+TD7 = TypedDict("TD7", {}, extra_items=InitVar[int])  # error: [invalid-type-form]
+TD8 = TypedDict("TD8", {}, extra_items=Final[int])  # error: [invalid-type-form]
+
+class TD9(TypedDict("TD9", {}, extra_items=Required[int])): ...  # error: [invalid-type-form]
+class TD10(TypedDict("TD9", {}, extra_items=Required[int])): ...  # error: [invalid-type-form]
+class TD11(TypedDict("TD9", {}, extra_items=Required[int])): ...  # error: [invalid-type-form]
+class TD12(TypedDict("TD9", {}, extra_items=Required[int])): ...  # error: [invalid-type-form]
+class TD13(TypedDict("TD9", {}, extra_items=Required[int])): ...  # error: [invalid-type-form]
 ```
 
 ## Function syntax with forward references
diff --git a/crates/ty_python_semantic/src/types/infer/builder.rs b/crates/ty_python_semantic/src/types/infer/builder.rs
index 0810b2cda1dcf..a4ee51fc72d8e 100644
--- a/crates/ty_python_semantic/src/types/infer/builder.rs
+++ b/crates/ty_python_semantic/src/types/infer/builder.rs
@@ -4199,22 +4199,17 @@ impl<'db, 'ast> TypeInferenceBuilder<'db, 'ast> {
                 });
 
                 match class_kind {
-                    Some(CodeGeneratorKind::TypedDict) => match qualifier {
-                        TypeQualifier::ClassVar | TypeQualifier::Final | TypeQualifier::InitVar => {
-                            let Some(builder) =
+                    Some(CodeGeneratorKind::TypedDict) => {
+                        if !qualifier.is_valid_in_typeddict_field()
+                            && let Some(builder) =
                                 self.context.report_lint(&INVALID_TYPE_FORM, annotation)
-                            else {
-                                continue;
-                            };
+                        {
                             builder.into_diagnostic(format_args!(
                                 "`{name}` is not allowed in TypedDict fields",
                                 name = qualifier.name()
                             ));
                         }
-                        TypeQualifier::NotRequired
-                        | TypeQualifier::ReadOnly
-                        | TypeQualifier::Required => {}
-                    },
+                    }
                     Some(CodeGeneratorKind::DataclassLike(_)) => match qualifier {
                         TypeQualifier::NotRequired
                         | TypeQualifier::ReadOnly
diff --git a/crates/ty_python_semantic/src/types/infer/builder/typed_dict.rs b/crates/ty_python_semantic/src/types/infer/builder/typed_dict.rs
index 6cc75bf77f1b1..ab90b41acfd1f 100644
--- a/crates/ty_python_semantic/src/types/infer/builder/typed_dict.rs
+++ b/crates/ty_python_semantic/src/types/infer/builder/typed_dict.rs
@@ -1,15 +1,19 @@
 use ruff_python_ast::name::Name;
 use ruff_python_ast::{self as ast, NodeIndex};
 use smallvec::SmallVec;
+use strum::IntoEnumIterator;
 
 use super::TypeInferenceBuilder;
+use crate::TypeQualifiers;
 use crate::semantic_index::definition::Definition;
 use crate::types::class::{ClassLiteral, DynamicTypedDictAnchor, DynamicTypedDictLiteral};
 use crate::types::diagnostic::{
-    INVALID_ARGUMENT_TYPE, MISSING_ARGUMENT, TOO_MANY_POSITIONAL_ARGUMENTS, UNKNOWN_ARGUMENT,
+    INVALID_ARGUMENT_TYPE, INVALID_TYPE_FORM, MISSING_ARGUMENT, TOO_MANY_POSITIONAL_ARGUMENTS,
+    UNKNOWN_ARGUMENT,
 };
+use crate::types::special_form::TypeQualifier;
 use crate::types::typed_dict::{TypedDictSchema, functional_typed_dict_field};
-use crate::types::{IntersectionType, KnownClass, Type, TypeContext};
+use crate::types::{IntersectionType, KnownClass, Type, TypeAndQualifiers, TypeContext};
 
 impl<'db> TypeInferenceBuilder<'db, '_> {
     /// Infer a `TypedDict(name, fields)` call expression.
@@ -124,7 +128,7 @@ impl<'db> TypeInferenceBuilder<'db, '_> {
                 }
                 "extra_items" => {
                     if definition.is_none() {
-                        self.infer_annotation_expression(&kw.value, self.deferred_state);
+                        self.infer_extra_items_kwarg(&kw.value);
                     }
                 }
                 unknown_kwarg => {
@@ -293,7 +297,7 @@ impl<'db> TypeInferenceBuilder<'db, '_> {
                 return TypedDictSchema::default();
             };
 
-            let annotation = self.infer_annotation_expression(&item.value, self.deferred_state);
+            let annotation = self.infer_typeddict_field(&item.value);
 
             schema.insert(
                 Name::new(key_literal.value(db)),
@@ -321,16 +325,54 @@ impl<'db> TypeInferenceBuilder<'db, '_> {
         if let Some(ast::Expr::Dict(dict_expr)) = arguments.args.get(1) {
             for ast::DictItem { key, value } in dict_expr {
                 if key.is_some() {
-                    self.infer_annotation_expression(value, self.deferred_state);
+                    self.infer_typeddict_field(value);
                 }
             }
         }
 
         if let Some(extra_items_kwarg) = arguments.find_keyword("extra_items") {
-            self.infer_annotation_expression(&extra_items_kwarg.value, self.deferred_state);
+            self.infer_extra_items_kwarg(&extra_items_kwarg.value);
         }
     }
 
+    fn infer_typeddict_field(&mut self, value: &ast::Expr) -> TypeAndQualifiers<'db> {
+        let annotation = self.infer_annotation_expression(value, self.deferred_state);
+        for qualifier in TypeQualifier::iter() {
+            if !qualifier.is_valid_in_typeddict_field()
+                && annotation
+                    .qualifiers
+                    .contains(TypeQualifiers::from(qualifier))
+                && let Some(builder) = self.context.report_lint(&INVALID_TYPE_FORM, value)
+            {
+                let mut diagnostic = builder.into_diagnostic(format_args!(
+                    "Type qualifier `{qualifier}` is not valid in a TypedDict field"
+                ));
+                diagnostic.info(
+                    "Only `Required`, `NotRequired` and `ReadOnly` are valid in this context",
+                );
+            }
+        }
+        annotation
+    }
+
+    fn infer_extra_items_kwarg(&mut self, value: &ast::Expr) -> TypeAndQualifiers<'db> {
+        let annotation = self.infer_annotation_expression(value, self.deferred_state);
+        for qualifier in TypeQualifier::iter() {
+            if qualifier != TypeQualifier::ReadOnly
+                && annotation
+                    .qualifiers
+                    .contains(TypeQualifiers::from(qualifier))
+                && let Some(builder) = self.context.report_lint(&INVALID_TYPE_FORM, value)
+            {
+                let mut diagnostic = builder.into_diagnostic(format_args!(
+                    "Type qualifier `{qualifier}` is not valid in a TypedDict `extra_items` argument"
+                ));
+                diagnostic.info("`ReadOnly` is the only permitted type qualifier here");
+            }
+        }
+        annotation
+    }
+
     /// Infer all non-type expressions in the `fields` argument of a functional `TypedDict` definition,
     /// and emit diagnostics for invalid field keys. Type expressions are not inferred during this pass,
     /// because it must be deferred for` TypedDict` definitions that may hold recursive references to
diff --git a/crates/ty_python_semantic/src/types/special_form.rs b/crates/ty_python_semantic/src/types/special_form.rs
index 2758c1adfe510..d1b1deeff5174 100644
--- a/crates/ty_python_semantic/src/types/special_form.rs
+++ b/crates/ty_python_semantic/src/types/special_form.rs
@@ -904,6 +904,13 @@ impl TypeQualifier {
             TypeQualifier::Final => true,
         }
     }
+
+    pub(crate) const fn is_valid_in_typeddict_field(self) -> bool {
+        match self {
+            TypeQualifier::ReadOnly | TypeQualifier::Required | TypeQualifier::NotRequired => true,
+            TypeQualifier::ClassVar | TypeQualifier::Final | TypeQualifier::InitVar => false,
+        }
+    }
 }
 
 impl From<TypeQualifier> for SpecialFormType {