diff --git a/r/tools/autobrew b/r/tools/autobrew
index 6ad8cf22374..07a68c50c7f 100644
--- a/r/tools/autobrew
+++ b/r/tools/autobrew
@@ -59,7 +59,7 @@ for FILE in $BREWDIR/Cellar/*/*/lib/*.a; do
   PKG_LIBS=`echo $PKG_LIBS | sed "s/-l$LIBNAME/-lbrew$LIBNAME/g"`
 done
 
-PKG_CFLAGS="-I$BREWDIR/opt/$PKG_BREW_NAME/include -DARROW_R_WITH_S3"
+PKG_CFLAGS="-I$BREWDIR/opt/$PKG_BREW_NAME/include"
 
 unset HOMEBREW_NO_ANALYTICS
 unset HOMEBREW_NO_AUTO_UPDATE