diff --git a/accounts-db/src/accounts_db.rs b/accounts-db/src/accounts_db.rs
index 9e60cabaacc..3f30b76604d 100644
--- a/accounts-db/src/accounts_db.rs
+++ b/accounts-db/src/accounts_db.rs
@@ -7053,6 +7053,15 @@ impl AccountsDb {
     }
 
     pub fn flush_accounts_cache_slot_for_tests(&self, slot: Slot) {
+        assert!(
+            self.accounts_index
+                .roots_tracker
+                .read()
+                .unwrap()
+                .alive_roots
+                .contains(&slot),
+            "slot: {slot}"
+        );
         self.flush_slot_cache_with_clean(slot, None::<&mut fn(&_) -> bool>, None);
     }
 
diff --git a/accounts-db/src/accounts_db/tests.rs b/accounts-db/src/accounts_db/tests.rs
index 05c24acc084..ce62b2e2189 100644
--- a/accounts-db/src/accounts_db/tests.rs
+++ b/accounts-db/src/accounts_db/tests.rs
@@ -6478,10 +6478,13 @@ fn test_mark_obsolete_accounts_at_startup_purge_slot() {
     // Store the same pubkey in multiple slots
     // Store other pubkey in slot0 to ensure slot is not purged
     accounts_db.store_for_tests((0, [(&pubkey1, &account), (&pubkey2, &account)].as_slice()));
+    accounts_db.add_root(0);
     accounts_db.flush_accounts_cache_slot_for_tests(0);
     accounts_db.store_for_tests((1, [(&pubkey1, &account)].as_slice()));
+    accounts_db.add_root(1);
     accounts_db.flush_accounts_cache_slot_for_tests(1);
     accounts_db.store_for_tests((2, [(&pubkey1, &account)].as_slice()));
+    accounts_db.add_root(2);
     accounts_db.flush_accounts_cache_slot_for_tests(2);
 
     let pubkeys_with_duplicates_by_bin = vec![vec![pubkey1]];
@@ -6514,6 +6517,7 @@ fn test_mark_obsolete_accounts_at_startup_multiple_bins() {
             slot,
             [(&pubkey1, &account), (&pubkey2, &account)].as_slice(),
         ));
+        accounts_db.add_root(slot);
         accounts_db.flush_accounts_cache_slot_for_tests(slot);
     }