diff --git a/docs/versionhistory.rst b/docs/versionhistory.rst
index 554b8b6..0bba890 100644
--- a/docs/versionhistory.rst
+++ b/docs/versionhistory.rst
@@ -19,6 +19,9 @@ This library adheres to
 - Fixed ``Self`` checks in instance/class methods that have positional-only arguments
 - Fixed explicit checks of PEP 604 unions against ``types.UnionType``
   (`#467 <https://github.com/agronholm/typeguard/issues/467>`_)
+- Fixed checks against annotations wrapped in ``NotRequired`` not being run unless the
+  ``NotRequired`` is a forward reference
+  (`#454 <https://github.com/agronholm/typeguard/issues/454>`_)
 
 **4.4.0** (2024-10-27)
 
diff --git a/src/typeguard/_checkers.py b/src/typeguard/_checkers.py
index 40ff2d4..5e34036 100644
--- a/src/typeguard/_checkers.py
+++ b/src/typeguard/_checkers.py
@@ -263,9 +263,10 @@ def check_typed_dict(
     for key, annotation in origin_type.__annotations__.items():
         if isinstance(annotation, ForwardRef):
             annotation = evaluate_forwardref(annotation, memo)
-            if get_origin(annotation) is NotRequired:
-                required_keys.discard(key)
-                annotation = get_args(annotation)[0]
+
+        if get_origin(annotation) is NotRequired:
+            required_keys.discard(key)
+            annotation = get_args(annotation)[0]
 
         type_hints[key] = annotation
 
diff --git a/tests/test_checkers.py b/tests/test_checkers.py
index d03080b..8653535 100644
--- a/tests/test_checkers.py
+++ b/tests/test_checkers.py
@@ -508,7 +508,8 @@ def test_notrequired_pass(self, typing_provider):
 
         class DummyDict(typing_provider.TypedDict):
             x: int
-            y: "NotRequired[int]"
+            y: NotRequired[int]
+            z: "NotRequired[int]"
 
         check_type({"x": 8}, DummyDict)
 
@@ -520,13 +521,19 @@ def test_notrequired_fail(self, typing_provider):
 
         class DummyDict(typing_provider.TypedDict):
             x: int
-            y: "NotRequired[int]"
+            y: NotRequired[int]
+            z: "NotRequired[int]"
 
         with pytest.raises(
             TypeCheckError, match=r"value of key 'y' of dict is not an instance of int"
         ):
             check_type({"x": 1, "y": "foo"}, DummyDict)
 
+        with pytest.raises(
+            TypeCheckError, match=r"value of key 'z' of dict is not an instance of int"
+        ):
+            check_type({"x": 1, "y": 6, "z": "foo"}, DummyDict)
+
     def test_is_typeddict(self, typing_provider):
         # Ensure both typing.TypedDict and typing_extensions.TypedDict are recognized
         class DummyDict(typing_provider.TypedDict):