diff --git a/.cirrus.yml b/.cirrus.yml
index 9d7ef969..8a51e1a7 100644
--- a/.cirrus.yml
+++ b/.cirrus.yml
@@ -92,10 +92,10 @@ test_task:
       - conda install --quiet --name base ${CONDA_CACHE_PACKAGES}
   nox_cache:
     folder: ${CIRRUS_WORKING_DIR}/.nox
+    reupload_on_changes: true
     fingerprint_script:
       - echo "${CIRRUS_TASK_NAME}"
-      - echo "$(date +%Y).$(expr $(date +%U) / ${CACHE_PERIOD}):${NOX_CACHE_BUILD}"
-      - sha256sum ${CIRRUS_WORKING_DIR}/requirements/py$(echo ${PY_VER} | tr -d ".").yml
+      - echo "${NOX_CACHE_BUILD}"
       - if [ -n "${IRIS_SOURCE}" ]; then echo "${IRIS_SOURCE}"; fi
   test_script:
     - export CONDA_OVERRIDE_LINUX="$(uname -r | cut -d'+' -f1)"
diff --git a/.github/workflows/refresh-lockfiles.yml b/.github/workflows/refresh-lockfiles.yml
new file mode 100644
index 00000000..5458eafb
--- /dev/null
+++ b/.github/workflows/refresh-lockfiles.yml
@@ -0,0 +1,42 @@
+# This workflow periodically creates new environment lock files based on the newest
+# available packages and dependencies.
+#
+# Environment specifications are given as conda environment.yml files found in
+# ``requirements/py**.yml``.  These state the packages required, the conda channels
+# that the packages will be pulled from, and any versions of packages that need to be
+# pinned at specific versions.
+#
+# For environments that have changed, a pull request will be made and submitted
+# to the master branch.
+
+name: Refresh Lockfiles
+
+
+on:
+  workflow_dispatch:
+  schedule:
+    # Run once a week on a Saturday night.
+    - cron: 1 0 * * 6
+
+
+jobs:
+
+  gen_lockfiles:
+    runs-on: ubuntu-latest
+    steps:
+      - uses: actions/checkout@v2
+      - name: install nox
+        run: |
+          source $CONDA/bin/activate base
+          conda install -y -c conda-forge nox pyyaml
+      - name: generate lockfiles
+        run: $CONDA/bin/nox --session update_lockfiles
+      - name: create pull request
+        uses: peter-evans/create-pull-request@052fc72b4198ba9fbc81b818c6e1859f747d49a8
+        with:
+          commit-message: Updated environment lockfiles
+          delete-branch: true
+          branch: auto-update-lockfiles
+          title: Update CI environment lockfiles
+          body: |
+            Lockfiles updated to the latest resolvable environment.
diff --git a/.pre-commit-config.yaml b/.pre-commit-config.yaml
index 2c1cbdb6..0833790f 100644
--- a/.pre-commit-config.yaml
+++ b/.pre-commit-config.yaml
@@ -2,7 +2,7 @@
 # See https://pre-commit.com/hooks.html for more hooks
 repos:
 -   repo: https://github.com/pre-commit/pre-commit-hooks
-    rev: 'v3.4.0'
+    rev: 'v4.0.1'
     hooks:
         # Prevent giant files from being committed.
     -   id: check-added-large-files
@@ -17,14 +17,14 @@ repos:
         # Don't commit to master branch.
     -   id: no-commit-to-branch
 -   repo: https://github.com/psf/black
-    rev: '20.8b1'
+    rev: '21.5b1'
     hooks:
     -   id: black
         # Force black to run on whole repo, using settings from pyproject.toml
         pass_filenames: false
         args: [--config=./pyproject.toml, .]
--   repo: https://gitlab.com/pycqa/flake8
-    rev: '3.9.0'
+-   repo: https://github.com/PyCQA/flake8
+    rev: '3.9.2'
     hooks:
         # Run flake8.
     -   id: flake8
diff --git a/noxfile.py b/noxfile.py
index 405ffa77..9a2eeeea 100644
--- a/noxfile.py
+++ b/noxfile.py
@@ -5,11 +5,13 @@
 
 """
 
-import hashlib
 import os
 from pathlib import Path
+import shutil
+from urllib.request import urlopen
 
 import nox
+from nox.logger import logger
 import yaml
 
 
@@ -30,68 +32,79 @@
 
 COVERAGE_PACKAGES = ["pytest-cov", "codecov"]
 IRIS_GITHUB = "https://github.com/scitools/iris.git"
+LOCKFILE_PLATFORM = "linux-64"
 
 
-def _cache_venv(session, fname):
+def _lockfile_path(py_string: str, platform_placeholder: bool = False) -> Path:
     """
-    Cache the nox session environment.
+    Return a constructed lockfile path for the relevant python string e.g ``py38``.
 
-    This consists of saving a hexdigest (sha256) of the associated
-    conda requirements YAML file.
-
-    Parameters
-    ----------
-    session: object
-        A `nox.sessions.Session` object.
-    fname: str
-        Requirements filename that defines this environment.
+    Optionally retain the ``{platform}`` placeholder to support conda-lock's
+    ``--filename-template``.
 
     """
-    python_version = session.python.replace(".", "")
-    with open(fname, "rb") as fi:
-        hexdigest = hashlib.sha256(fi.read()).hexdigest()
+    dir = Path() / "requirements" / "nox.lock"
+    name_template = "{py_string}-{platform}.lock"
+    if platform_placeholder:
+        platform = "{platform}"
+    else:
+        platform = LOCKFILE_PLATFORM
+    lockfile_name = name_template.format(py_string=py_string, platform=platform)
+    return dir / lockfile_name
+
+
+def _session_lockfile(session: nox.sessions.Session) -> Path:
+    """Return the path of the session lockfile."""
+    return _lockfile_path(py_string=f"py{session.python.replace('.', '')}")
+
+
+def _file_content(file_path: Path) -> str:
+    with file_path.open("r") as file:
+        return file.read()
+
+
+def _session_cachefile(session: nox.sessions.Session) -> Path:
+    """Return the path of the session lockfile cache."""
     tmp_dir = Path(session.create_tmp())
-    cache = tmp_dir / f"py{python_version}.sha"
-    with open(cache, "w") as fo:
-        fo.write(hexdigest)
+    cache = tmp_dir / _session_lockfile(session).name
+    return cache
+
+
+def _venv_populated(session: nox.sessions.Session) -> bool:
+    """Return True if the Conda venv has been created and the list of packages in the lockfile installed."""
+    return _session_cachefile(session).is_file()
 
 
-def _combine_requirements(primary, secondary, ignore=None):
+def _venv_changed(session: nox.sessions.Session) -> bool:
+    """Return True if the installed session is different to that specified in the lockfile."""
+    result = False
+    if _venv_populated(session):
+        expected = _file_content(_session_lockfile(session))
+        actual = _file_content(_session_cachefile(session))
+        result = actual != expected
+    return result
+
+
+def _install_and_cache_venv(session: nox.sessions.Session) -> None:
     """
-    Combine the conda environment YAML files together into one.
+    Cache the nox session environment.
+
+    This consists of saving a hexdigest (sha256) of the associated
+    Conda lock file.
 
     Parameters
     ----------
-    primary: str
-        The filename of the primary YAML conda environment.
-    secondary: str
-        The filename of the subordinate YAML conda environment.
-    ignore: str, optional
-        The prefix of any package name to be ignored from the
-        combined dependency requirements.
-
-    Returns
-    -------
-    dict
-        A dictionary of the combined YAML conda environments.
+    session: object
+        A `nox.sessions.Session` object.
 
     """
-    with open(primary, "r") as fi:
-        result = yaml.load(fi, Loader=yaml.FullLoader)
-    with open(secondary, "r") as fi:
-        secondary = yaml.load(fi, Loader=yaml.FullLoader)
-    # Combine the channels and dependencies only.
-    for key in ["channels", "dependencies"]:
-        result[key] = sorted(set(result[key]).union(secondary[key]))
-    if ignore:
-        # Filter out any specific prefixed package dependencies.
-        result["dependencies"] = [
-            spec for spec in result["dependencies"] if not spec.startswith(ignore)
-        ]
-    return result
+    lockfile = _session_lockfile(session)
+    session.conda_install(f"--file={lockfile}")
+    with _session_cachefile(session).open("w") as cachefile:
+        cachefile.write(_file_content(lockfile))
 
 
-def _get_iris_github_artifact(session):
+def _get_iris_github_artifact(session: nox.sessions.Session) -> str:
     """
     Determine whether an Iris source artifact from GitHub is required.
 
@@ -109,6 +122,14 @@ def _get_iris_github_artifact(session):
 
     """
     result = IRIS_SOURCE
+    if not result:
+        # .cirrus.yml sets IRIS_SOURCE. Need to fetch the value (if any) when
+        # called outside Cirrus (e.g. user, ASV).
+        # .cirrus.yml = single-source-of-truth.
+        with Path(".cirrus.yml").open("r") as file:
+            cirrus_config = yaml.load(file, Loader=yaml.FullLoader)
+        result = cirrus_config["env"].get("IRIS_SOURCE", None)
+
     # The CLI overrides the environment variable.
     for arg in session.posargs:
         if arg.startswith("--iris="):
@@ -130,34 +151,126 @@ def _get_iris_github_artifact(session):
     return result
 
 
-def _venv_cached(session, fname):
+def _prepare_env(session: nox.sessions.Session) -> None:
+    venv_dir = session.virtualenv.location_name
+
+    if not _venv_populated(session):
+        # Environment has been created but packages not yet installed.
+        # Populate the environment from the lockfile.
+        logger.debug(f"Populating conda env: {venv_dir}")
+        _install_and_cache_venv(session)
+
+    elif _venv_changed(session):
+        # Destroy the environment and rebuild it.
+        logger.debug(f"Lockfile changed. Recreating conda env: {venv_dir}")
+        _reuse_original = session.virtualenv.reuse_existing
+        session.virtualenv.reuse_existing = False
+        session.virtualenv.create()
+        _install_and_cache_venv(session)
+        session.virtualenv.reuse_existing = _reuse_original
+
+    logger.debug(f"Environment up to date: {venv_dir}")
+
+    iris_artifact = _get_iris_github_artifact(session)
+    if iris_artifact:
+        # Install the iris source in develop mode.
+        tmp_dir = Path(session.create_tmp())
+        iris_dir = tmp_dir / "iris"
+        cwd = Path.cwd()
+        if not iris_dir.is_dir():
+            session.run_always(
+                "git", "clone", IRIS_GITHUB, str(iris_dir), external=True
+            )
+        session.cd(str(iris_dir))
+        session.run_always("git", "fetch", "origin", external=True)
+        session.run_always("git", "checkout", iris_artifact, external=True)
+        session.cd(str(cwd))
+        session.install("--no-deps", "--editable", str(iris_dir))
+
+    # Determine whether verbose diagnostics have been requested
+    # from the command line.
+    verbose = "-v" in session.posargs or "--verbose" in session.posargs
+
+    if verbose:
+        session.run_always("conda", "info")
+        session.run_always("conda", "list", f"--prefix={venv_dir}")
+        session.run_always(
+            "conda",
+            "list",
+            f"--prefix={venv_dir}",
+            "--explicit",
+        )
+
+
+@nox.session
+def update_lockfiles(session: nox.sessions.Session):
     """
-    Determine whether the nox session environment has been cached.
+    Re-resolve env specs and store as lockfiles (``requirements/nox.lock/``).
+
+    Original Conda environment specifications are at:
+    ``requirements/py**.yml``. The output lock files denote the dependencies
+    that iris-esmf-regrid is tested against, and therefore officially supports.
 
     Parameters
     ----------
     session: object
-        A `nox.sessions.Session` object.
-    fname: str
-        Requirements filename that defines this environment.
-
-    Returns
-    -------
-    bool
-        Whether the session has been cached.
+        A `nox.sessions.Session object.
 
     """
-    result = False
-    tmp_dir = Path(session.create_tmp())
-    python_version = session.python.replace(".", "")
-    cache = tmp_dir / f"py{python_version}.sha"
-    if cache.is_file():
-        with open(fname, "rb") as fi:
-            hexdigest = hashlib.sha256(fi.read()).hexdigest()
-        with open(cache, "r") as fi:
-            cached = fi.read()
-        result = cached == hexdigest
-    return result
+    session.install("conda-lock")
+
+    for req_file in Path("requirements").glob(r"py[0-9]*.yml"):
+        python_string = req_file.stem
+
+        # Generate the appropriate conda-lock template name, keeping the {platform}
+        # placeholder to support conda-lock's internals.
+        filename_template = _lockfile_path(python_string, platform_placeholder=True)
+        lockfile_path = _lockfile_path(python_string, platform_placeholder=False)
+        # Create the parent directory if it doesn't already exist.
+        try:
+            filename_template.parent.mkdir()
+        except FileExistsError:
+            pass
+
+        # Use a copy of the requirements file in a tmp dir - the file will
+        # be modified if installing a custom Iris checkout.
+        tmp_dir = Path(session.create_tmp())
+        req_file_local = tmp_dir / req_file.name
+        shutil.copy(req_file, req_file_local)
+
+        conda_lock_cmd = [
+            "conda-lock",
+            "lock",
+            f"--filename-template={filename_template}",
+            f"--file={req_file_local}",
+            f"--platform={LOCKFILE_PLATFORM}",
+        ]
+
+        # Get the requirements for Iris too, if an Iris checkout is specified.
+        iris_artifact = _get_iris_github_artifact(session)
+        if iris_artifact:
+            # Remove ``iris`` from dependencies, if present.
+            with req_file_local.open("r+") as file:
+                reqs = yaml.load(file, Loader=yaml.FullLoader)
+                reqs["dependencies"] = [
+                    spec for spec in reqs["dependencies"] if not spec.startswith("iris")
+                ]
+                yaml.dump(reqs, file)
+
+            iris_req_name = f"{python_string}.yml"
+            iris_req_url = (
+                f"https://raw.githubusercontent.com/SciTools/iris/"
+                f"{iris_artifact}/requirements/ci/{iris_req_name}"
+            )
+            iris_req_file = (tmp_dir / iris_req_name).with_stem(f"{python_string}-iris")
+            iris_req = urlopen(iris_req_url).read()
+            with iris_req_file.open("wb") as file:
+                file.write(iris_req)
+            # Conda-lock can resolve multiple requirements files together.
+            conda_lock_cmd.append(f"--file={iris_req_file}")
+
+        session.run(*conda_lock_cmd, silent=True)
+        print(f"Conda lock file created: {lockfile_path}")
 
 
 @nox.session
@@ -208,71 +321,11 @@ def tests(session):
     session: object
         A `nox.sessions.Session` object.
 
-    Notes
-    -----
-    See
-      - https://github.com/theacodes/nox/issues/346
-      - https://github.com/theacodes/nox/issues/260
-
     """
-    artifact = _get_iris_github_artifact(session)
-    python_version = session.python.replace(".", "")
-    requirements_fname = f"requirements/py{python_version}.yml"
-
-    if artifact:
-        tmp_dir = Path(session.create_tmp())
-        artifact_dir = tmp_dir / "iris"
-        cwd = Path.cwd()
-        if not artifact_dir.is_dir():
-            session.run("git", "clone", IRIS_GITHUB, str(artifact_dir), external=True)
-        session.cd(artifact_dir)
-        session.run("git", "fetch", "origin", external=True)
-        session.run("git", "checkout", artifact, external=True)
-        session.cd(str(cwd))
-        iris_requirements_fname = (
-            f"{artifact_dir}/requirements/ci/py{python_version}.yml"
-        )
-        requirements_yaml = _combine_requirements(
-            requirements_fname, iris_requirements_fname, ignore="iris"
-        )
-        requirements_fname = tmp_dir / "requirements.yml"
-        with open(requirements_fname, "w") as fo:
-            yaml.dump(requirements_yaml, fo)
-
-    # Install the package requirements.
-    if not _venv_cached(session, requirements_fname):
-        # Back-door approach to force nox to use "conda env update".
-        command = (
-            "conda",
-            "env",
-            "update",
-            f"--prefix={session.virtualenv.location}",
-            f"--file={requirements_fname}",
-            "--prune",
-        )
-        session._run(*command, silent=True, external="error")
-        _cache_venv(session, requirements_fname)
-
-    if artifact:
-        # Install the iris source in develop mode.
-        session.install("--no-deps", "--editable", str(artifact_dir))
-
+    _prepare_env(session)
     # Install the esmf-regrid source in develop mode.
     session.install("--no-deps", "--editable", ".")
 
-    # Determine whether verbose diagnostics have been requested from the command line.
-    verbose = "-v" in session.posargs or "--verbose" in session.posargs
-
-    if verbose:
-        session.run("conda", "info")
-        session.run("conda", "list", f"--prefix={session.virtualenv.location}")
-        session.run(
-            "conda",
-            "list",
-            f"--prefix={session.virtualenv.location}",
-            "--explicit",
-        )
-
     if COVERAGE:
         # Execute the tests with code coverage.
         session.conda_install("--channel=conda-forge", *COVERAGE_PACKAGES)
diff --git a/pyproject.toml b/pyproject.toml
index 6e9c4517..60fa52ab 100644
--- a/pyproject.toml
+++ b/pyproject.toml
@@ -10,6 +10,7 @@ exclude = '''
     | \.git          # root of the project
     | \.hg
     | \.mypy_cache
+    | \.nox
     | \.tox
     | \.venv
     | _build
diff --git a/requirements/nox.lock/py36-linux-64.lock b/requirements/nox.lock/py36-linux-64.lock
new file mode 100644
index 00000000..002de9db
--- /dev/null
+++ b/requirements/nox.lock/py36-linux-64.lock
@@ -0,0 +1,250 @@
+# platform: linux-64
+# env_hash: 8d4eabba93d707c1be9daf57e71eb8926194a98edf2ed574d471346ab3db9f66
+@EXPLICIT
+https://conda.anaconda.org/conda-forge/linux-64/_libgcc_mutex-0.1-conda_forge.tar.bz2#d7c89558ba9fa0495403155b64376d81
+https://conda.anaconda.org/conda-forge/linux-64/ca-certificates-2020.12.5-ha878542_0.tar.bz2#7eb5d4ffeee663caa1635cd67071bc1b
+https://conda.anaconda.org/conda-forge/noarch/font-ttf-dejavu-sans-mono-2.37-hab24e00_0.tar.bz2#0c96522c6bdaed4b1566d11387caaf45
+https://conda.anaconda.org/conda-forge/noarch/font-ttf-inconsolata-3.000-h77eed37_0.tar.bz2#34893075a5c9e55cdafac56607368fc6
+https://conda.anaconda.org/conda-forge/noarch/font-ttf-source-code-pro-2.038-h77eed37_0.tar.bz2#4d59c254e01d9cde7957100457e2d5fb
+https://conda.anaconda.org/conda-forge/noarch/font-ttf-ubuntu-0.83-hab24e00_0.tar.bz2#19410c3df09dfb12d1206132a1d357c5
+https://conda.anaconda.org/conda-forge/linux-64/ld_impl_linux-64-2.35.1-hea4e1c9_2.tar.bz2#83610dba766a186bdc7a116053b782a4
+https://conda.anaconda.org/conda-forge/linux-64/libgfortran5-9.3.0-hff62375_19.tar.bz2#c2d8da3cb171e4aa642d20c6e4e42a04
+https://conda.anaconda.org/conda-forge/linux-64/libstdcxx-ng-9.3.0-h6de172a_19.tar.bz2#cd9a24a8dde03ec0cf0e603b0bea85a1
+https://conda.anaconda.org/conda-forge/linux-64/mpi-1.0-mpich.tar.bz2#c1fcff3417b5a22bbc4cf6e8c23648cf
+https://conda.anaconda.org/conda-forge/linux-64/mysql-common-8.0.23-ha770c72_2.tar.bz2#ce876d0c998e1e2eb1dc67b01937737f
+https://conda.anaconda.org/conda-forge/noarch/fonts-conda-forge-1-0.tar.bz2#f766549260d6815b0c52253f1fb1bb29
+https://conda.anaconda.org/conda-forge/linux-64/libgfortran-ng-9.3.0-hff62375_19.tar.bz2#aea379bd68fdcdf9499fa1453f852ac1
+https://conda.anaconda.org/conda-forge/linux-64/libgomp-9.3.0-h2828fa1_19.tar.bz2#ab0a307912033126da02507b59e79ec9
+https://conda.anaconda.org/conda-forge/linux-64/_openmp_mutex-4.5-1_gnu.tar.bz2#561e277319a41d4f24f5c05a9ef63c04
+https://conda.anaconda.org/conda-forge/noarch/fonts-conda-ecosystem-1-0.tar.bz2#fee5683a3f04bd15cbd8318b096a27ab
+https://conda.anaconda.org/conda-forge/linux-64/libgcc-ng-9.3.0-h2828fa1_19.tar.bz2#9d5cdfc51476ee4dcdd96ed2dca3f943
+https://conda.anaconda.org/conda-forge/linux-64/alsa-lib-1.2.3-h516909a_0.tar.bz2#1378b88874f42ac31b2f8e4f6975cb7b
+https://conda.anaconda.org/conda-forge/linux-64/bzip2-1.0.8-h7f98852_4.tar.bz2#a1fd65c7ccbf10880423d82bca54eb54
+https://conda.anaconda.org/conda-forge/linux-64/c-ares-1.17.1-h7f98852_1.tar.bz2#ed1dc233ed5e3eaa9bfbaac64d130c5e
+https://conda.anaconda.org/conda-forge/linux-64/expat-2.3.0-h9c3ff4c_0.tar.bz2#1fb8f0254eb78a2a0c120155e1b1a207
+https://conda.anaconda.org/conda-forge/linux-64/fribidi-1.0.10-h36c2ea0_0.tar.bz2#ac7bc6a654f8f41b352b38f4051135f8
+https://conda.anaconda.org/conda-forge/linux-64/geos-3.9.1-h9c3ff4c_2.tar.bz2#b9a6d9422aed3ad84ec6ccee9bfcaa0f
+https://conda.anaconda.org/conda-forge/linux-64/giflib-5.2.1-h36c2ea0_2.tar.bz2#626e68ae9cc5912d6adb79d318cf962d
+https://conda.anaconda.org/conda-forge/linux-64/graphite2-1.3.13-h58526e2_1001.tar.bz2#8c54672728e8ec6aa6db90cf2806d220
+https://conda.anaconda.org/conda-forge/linux-64/icu-68.1-h58526e2_0.tar.bz2#fc7a4271dc2a7f4fd78cd63695baf7c3
+https://conda.anaconda.org/conda-forge/linux-64/jpeg-9d-h36c2ea0_0.tar.bz2#ea02ce6037dbe81803ae6123e5ba1568
+https://conda.anaconda.org/conda-forge/linux-64/libev-4.33-h516909a_1.tar.bz2#6f8720dff19e17ce5d48cfe7f3d2f0a3
+https://conda.anaconda.org/conda-forge/linux-64/libffi-3.3-h58526e2_2.tar.bz2#665369991d8dd290ac5ee92fce3e6bf5
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diff --git a/requirements/nox.lock/py37-linux-64.lock b/requirements/nox.lock/py37-linux-64.lock
new file mode 100644
index 00000000..b29961be
--- /dev/null
+++ b/requirements/nox.lock/py37-linux-64.lock
@@ -0,0 +1,247 @@
+# platform: linux-64
+# env_hash: 19592b2ceed3876b76de94d6ea4c1c0c36f92e13e488824bc5c7565d7d0cbfd2
+@EXPLICIT
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diff --git a/requirements/nox.lock/py38-linux-64.lock b/requirements/nox.lock/py38-linux-64.lock
new file mode 100644
index 00000000..6e33d086
--- /dev/null
+++ b/requirements/nox.lock/py38-linux-64.lock
@@ -0,0 +1,245 @@
+# platform: linux-64
+# env_hash: dc1bd2ccaf71fc80c7236cc2698f4603d58aaac51fa1d39deb4bb9db9369fa76
+@EXPLICIT
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