diff --git a/.changeset/afraid-mugs-priest.md b/.changeset/afraid-mugs-priest.md
new file mode 100644
index 0000000000000..d4faa51b11da7
--- /dev/null
+++ b/.changeset/afraid-mugs-priest.md
@@ -0,0 +1,7 @@
+---
+"@rocket.chat/meteor": patch
+"@rocket.chat/core-typings": patch
+"@rocket.chat/livechat": patch
+---
+
+Fixes issue causing the setDepartment Livechat API overriding some triggers conditions
diff --git a/packages/livechat/src/lib/hooks.ts b/packages/livechat/src/lib/hooks.ts
index 79cb3129dd66a..7e3e56625761a 100644
--- a/packages/livechat/src/lib/hooks.ts
+++ b/packages/livechat/src/lib/hooks.ts
@@ -49,6 +49,18 @@ const updateIframeGuestData = (data: Partial<StoreState['guest']>) => {
 
 export type HooksWidgetAPI = typeof api;
 
+const updateIframeData = (data: Partial<StoreState['iframe']>) => {
+	const { iframe } = store.state;
+
+	if (data.guest) {
+		throw new Error('Guest data changes not allowed. Use updateIframeGuestData instead.');
+	}
+
+	const iframeData = { ...iframe, ...data };
+
+	store.setState({ iframe: { ...iframeData } });
+};
+
 const api = {
 	pageVisited(info: { change: string; title: string; location: { href: string } }) {
 		const { token, room } = store.state;
@@ -90,6 +102,11 @@ const api = {
 			defaultAgent,
 		} = store.state;
 
+		if (!user) {
+			updateIframeData({ defaultDepartment: value });
+			return;
+		}
+
 		const { department: existingDepartment } = user || {};
 
 		const department = departments.find((dep) => dep._id === value || dep.name === value)?._id || '';
diff --git a/packages/livechat/src/routes/Register/index.tsx b/packages/livechat/src/routes/Register/index.tsx
index c752281e7372b..2d88c7e1e5ff7 100644
--- a/packages/livechat/src/routes/Register/index.tsx
+++ b/packages/livechat/src/routes/Register/index.tsx
@@ -44,7 +44,9 @@ export const Register: FunctionalComponent<{ path: string }> = () => {
 			theme: { title },
 			customFields = [],
 		},
-		iframe: { guest: { department: guestDepartment = undefined, name: guestName = undefined, email: guestEmail = undefined } = {} },
+
+		iframe: { defaultDepartment, guest: { name: guestName = undefined, email: guestEmail = undefined } = {} },
+
 		loading = false,
 		token,
 		dispatch,
@@ -96,8 +98,9 @@ export const Register: FunctionalComponent<{ path: string }> = () => {
 	};
 
 	const getDepartmentDefault = () => {
-		if (departments.some((dept) => dept._id === guestDepartment)) {
-			return guestDepartment;
+		const dept = departments.find((dept) => dept._id === defaultDepartment || dept.name === defaultDepartment);
+		if (dept?._id) {
+			return dept._id;
 		}
 	};
 
diff --git a/packages/livechat/src/store/index.tsx b/packages/livechat/src/store/index.tsx
index 06a8f05c4d060..4725a435aad56 100644
--- a/packages/livechat/src/store/index.tsx
+++ b/packages/livechat/src/store/index.tsx
@@ -72,6 +72,7 @@ export type StoreState = {
 		visible?: boolean;
 		department?: string;
 		language?: string;
+		defaultDepartment?: string;
 	};
 	gdpr: {
 		accepted: boolean;