diff --git a/qiskit/circuit/__init__.py b/qiskit/circuit/__init__.py
index 3f9afacaef5b..140415e7988f 100644
--- a/qiskit/circuit/__init__.py
+++ b/qiskit/circuit/__init__.py
@@ -234,7 +234,6 @@
 from .controlledgate import ControlledGate
 from .instruction import Instruction
 from .instructionset import InstructionSet
-from .operation import Operation
 from .barrier import Barrier
 from .delay import Delay
 from .measure import Measure
diff --git a/qiskit/circuit/barrier.py b/qiskit/circuit/barrier.py
index 08395d4d4d3c..c10b32069595 100644
--- a/qiskit/circuit/barrier.py
+++ b/qiskit/circuit/barrier.py
@@ -14,10 +14,9 @@
 
 from qiskit.exceptions import QiskitError
 from .instruction import Instruction
-from .operation import Operation
 
 
-class Barrier(Instruction, Operation):
+class Barrier(Instruction):
     """Barrier instruction."""
 
     _directive = True
diff --git a/qiskit/circuit/gate.py b/qiskit/circuit/gate.py
index 2e4970e4b0bc..b271aa161c82 100644
--- a/qiskit/circuit/gate.py
+++ b/qiskit/circuit/gate.py
@@ -18,10 +18,9 @@
 from qiskit.circuit.parameterexpression import ParameterExpression
 from qiskit.circuit.exceptions import CircuitError
 from .instruction import Instruction
-from .operation import Operation
 
 
-class Gate(Instruction, Operation):
+class Gate(Instruction):
     """Unitary gate."""
 
     def __init__(
diff --git a/qiskit/circuit/measure.py b/qiskit/circuit/measure.py
index 57e4c5e4db12..8da0db586c0d 100644
--- a/qiskit/circuit/measure.py
+++ b/qiskit/circuit/measure.py
@@ -17,11 +17,10 @@
 import warnings
 
 from qiskit.circuit.instruction import Instruction
-from qiskit.circuit.operation import Operation
 from qiskit.circuit.exceptions import CircuitError
 
 
-class Measure(Instruction, Operation):
+class Measure(Instruction):
     """Quantum measurement in the computational basis."""
 
     def __init__(self):
diff --git a/qiskit/circuit/reset.py b/qiskit/circuit/reset.py
index b5a36c51f7a1..e986c1ba3390 100644
--- a/qiskit/circuit/reset.py
+++ b/qiskit/circuit/reset.py
@@ -17,10 +17,9 @@
 import warnings
 
 from qiskit.circuit.instruction import Instruction
-from qiskit.circuit.operation import Operation
 
 
-class Reset(Instruction, Operation):
+class Reset(Instruction):
     """Qubit reset."""
 
     def __init__(self):
diff --git a/qiskit/extensions/quantum_initializer/initializer.py b/qiskit/extensions/quantum_initializer/initializer.py
index 69f79c881df1..8382fa62f846 100644
--- a/qiskit/extensions/quantum_initializer/initializer.py
+++ b/qiskit/extensions/quantum_initializer/initializer.py
@@ -17,13 +17,13 @@
 
 from qiskit.circuit import QuantumCircuit
 from qiskit.circuit import QuantumRegister
-from qiskit.circuit import Instruction, Operation
+from qiskit.circuit import Instruction
 from qiskit.circuit.library.data_preparation import StatePreparation
 
 _EPS = 1e-10  # global variable used to chop very small numbers to zero
 
 
-class Initialize(Instruction, Operation):
+class Initialize(Instruction):
     """Complex amplitude initialization.
 
     Class that initializes some flexible collection of qubit registers, implemented by calling
diff --git a/qiskit/extensions/quantum_initializer/isometry.py b/qiskit/extensions/quantum_initializer/isometry.py
index 31debc6a44fe..cb3bb1f12311 100644
--- a/qiskit/extensions/quantum_initializer/isometry.py
+++ b/qiskit/extensions/quantum_initializer/isometry.py
@@ -25,7 +25,6 @@
 
 from qiskit.circuit.exceptions import CircuitError
 from qiskit.circuit.instruction import Instruction
-from qiskit.circuit.operation import Operation
 from qiskit.circuit.quantumcircuit import QuantumCircuit
 from qiskit.circuit.quantumregister import QuantumRegister
 from qiskit.exceptions import QiskitError
@@ -36,7 +35,7 @@
 _EPS = 1e-10  # global variable used to chop very small numbers to zero
 
 
-class Isometry(Instruction, Operation):
+class Isometry(Instruction):
     """
     Decomposition of arbitrary isometries from m to n qubits. In particular, this allows to
     decompose unitaries (m=n) and to do state preparation (m=0).
diff --git a/qiskit/quantum_info/operators/dihedral/dihedral.py b/qiskit/quantum_info/operators/dihedral/dihedral.py
index 3e2f0c32b975..9116d1ad77e9 100644
--- a/qiskit/quantum_info/operators/dihedral/dihedral.py
+++ b/qiskit/quantum_info/operators/dihedral/dihedral.py
@@ -23,12 +23,12 @@
 from qiskit.quantum_info.operators.scalar_op import ScalarOp
 from qiskit.quantum_info.synthesis.cnotdihedral_decompose import decompose_cnotdihedral
 from qiskit.quantum_info.operators.mixins import generate_apidocs, AdjointMixin
-from qiskit.circuit import QuantumCircuit, Instruction, Operation
+from qiskit.circuit import QuantumCircuit, Instruction
 from .dihedral_circuits import _append_circuit
 from .polynomial import SpecialPolynomial
 
 
-class CNOTDihedral(BaseOperator, AdjointMixin, Operation):
+class CNOTDihedral(BaseOperator, AdjointMixin):
     """An N-qubit operator from the CNOT-Dihedral group.
 
      The CNOT-Dihedral group is generated by the quantum gates,
diff --git a/qiskit/quantum_info/operators/symplectic/clifford.py b/qiskit/quantum_info/operators/symplectic/clifford.py
index cd4a48c88e6e..90b0ec2d3503 100644
--- a/qiskit/quantum_info/operators/symplectic/clifford.py
+++ b/qiskit/quantum_info/operators/symplectic/clifford.py
@@ -16,7 +16,7 @@
 import numpy as np
 
 from qiskit.exceptions import QiskitError
-from qiskit.circuit import QuantumCircuit, Instruction, Operation
+from qiskit.circuit import QuantumCircuit, Instruction
 from qiskit.circuit.library.standard_gates import IGate, XGate, YGate, ZGate, HGate, SGate
 from qiskit.quantum_info.operators.base_operator import BaseOperator
 from qiskit.quantum_info.operators.operator import Operator
@@ -27,7 +27,7 @@
 from .clifford_circuits import _append_circuit
 
 
-class Clifford(BaseOperator, AdjointMixin, Operation):
+class Clifford(BaseOperator, AdjointMixin):
     """An N-qubit unitary operator from the Clifford group.
 
     **Representation**
diff --git a/qiskit/quantum_info/operators/symplectic/pauli.py b/qiskit/quantum_info/operators/symplectic/pauli.py
index 5549e210b618..9334731fb872 100644
--- a/qiskit/quantum_info/operators/symplectic/pauli.py
+++ b/qiskit/quantum_info/operators/symplectic/pauli.py
@@ -20,7 +20,7 @@
 
 import numpy as np
 
-from qiskit.circuit import Instruction, QuantumCircuit, Operation
+from qiskit.circuit import Instruction, QuantumCircuit
 from qiskit.circuit.barrier import Barrier
 from qiskit.circuit.library.generalized_gates import PauliGate
 from qiskit.circuit.library.standard_gates import IGate, XGate, YGate, ZGate
@@ -31,7 +31,7 @@
 from qiskit.utils.deprecation import deprecate_function
 
 
-class Pauli(BasePauli, Operation):
+class Pauli(BasePauli):
     r"""N-qubit Pauli operator.
 
     This class represents an operator :math:`P` from the full :math:`n`-qubit
diff --git a/releasenotes/notes/Operation-abstract-base-class-99f46180c7ddaa6b.yaml b/releasenotes/notes/Operation-abstract-base-class-99f46180c7ddaa6b.yaml
deleted file mode 100644
index b4d13418d7ad..000000000000
--- a/releasenotes/notes/Operation-abstract-base-class-99f46180c7ddaa6b.yaml
+++ /dev/null
@@ -1,10 +0,0 @@
----
-features:
-  - |
-    A new :class:`~qiskit.circuit.Operation` base class for objects
-    that can be added to a :class:`~qiskit.circuit.QuantumCircuit`.
-    These objects include :class:`~qiskit.circuit.Gate`, :class:`~qiskit.circuit.Reset`,
-    :class:`~qiskit.circuit.Barrier`, :class:`~qiskit.circuit.Measure`,
-    and operators such as :class:`~qiskit.quantum_info.Clifford`.
-    The main purpose is to add an :class:`~qiskit.circuit.Operation` to a
-    :class:`~qiskit.circuit.QuantumCircuit` without synthesizing it before the transpilation.