EGFR MET Barcoded Amplicons Validation

This dataset consists of two smartcells with two movies each in bas.h5 format. Each target amplicon in the EGFR-MET Fluidigm Array is barcoded with a different barcode pair from the standard PacBio 16bp barcode set (48 pairs).

Validation for barcodes can be done by resequencing these data against the EGFR_MET reference dataset. Each barcode should map to only a single target amplicon.

• The data can be downloaded here.

• More information on barocding with PacBio can be found here.

• More information on the EGFR-MET dataset as used in Pacbio can be found here.