diff --git a/pkgs/development/python-modules/python-magic/default.nix b/pkgs/development/python-modules/python-magic/default.nix
index 056b466216bfc..890cfb50c2cc7 100644
--- a/pkgs/development/python-modules/python-magic/default.nix
+++ b/pkgs/development/python-modules/python-magic/default.nix
@@ -29,6 +29,14 @@ buildPythonPackage rec {
       url = "https://github.com/ahupp/python-magic/commit/4ffcd59113fa26d7c2e9d5897b1eef919fd4b457.patch";
       hash = "sha256-67GpjlGiR4/os/iZ69V+ZziVLpjmid+7t+gQ2aQy9I0=";
     })
+
+    # Upstream patch to amend test suite for-5.45:
+    #   https://github.com/ahupp/python-magic/pull/290
+    (fetchpatch {
+      name = "file-5.45.patch";
+      url = "https://github.com/ahupp/python-magic/commit/3d2405ca80cd39b2a91decd26af81dcf181390a4.patch";
+      hash = "sha256-HRsnO9MGfMD9BkJdC4SrEFQ1OZEaXpwakXFLoaCPK94=";
+    })
   ];
 
   preCheck = ''