diff --git a/nixos/modules/config/pulseaudio.nix b/nixos/modules/config/pulseaudio.nix
index 01555d28b73fb..4cc6c4df74631 100644
--- a/nixos/modules/config/pulseaudio.nix
+++ b/nixos/modules/config/pulseaudio.nix
@@ -17,10 +17,6 @@ let
   binary = "${getBin overriddenPackage}/bin/pulseaudio";
   binaryNoDaemon = "${binary} --daemonize=no";
 
-  # Forces 32bit pulseaudio and alsa-plugins to be built/supported for apps
-  # using 32bit alsa on 64bit linux.
-  enable32BitAlsaPlugins = cfg.support32Bit && stdenv.isx86_64 && (pkgs.pkgsi686Linux.alsa-lib != null && pkgs.pkgsi686Linux.libpulseaudio != null);
-
 
   myConfigFile =
     let
@@ -60,26 +56,22 @@ let
   # Write an /etc/asound.conf that causes all ALSA applications to
   # be re-routed to the PulseAudio server through ALSA's Pulse
   # plugin.
-  alsaConf = writeText "asound.conf" (''
+  alsaConf = writeText "asound.conf" ''
     pcm_type.pulse {
-      libs.native = ${pkgs.alsa-plugins}/lib/alsa-lib/libasound_module_pcm_pulse.so ;
-      ${lib.optionalString enable32BitAlsaPlugins
-     "libs.32Bit = ${pkgs.pkgsi686Linux.alsa-plugins}/lib/alsa-lib/libasound_module_pcm_pulse.so ;"}
+      lib ${pkgs.alsa-plugins}/lib/alsa-lib/libasound_module_pcm_pulse.so ;
     }
     pcm.!default {
       type pulse
       hint.description "Default Audio Device (via PulseAudio)"
     }
     ctl_type.pulse {
-      libs.native = ${pkgs.alsa-plugins}/lib/alsa-lib/libasound_module_ctl_pulse.so ;
-      ${lib.optionalString enable32BitAlsaPlugins
-     "libs.32Bit = ${pkgs.pkgsi686Linux.alsa-plugins}/lib/alsa-lib/libasound_module_ctl_pulse.so ;"}
+      lib ${pkgs.alsa-plugins}/lib/alsa-lib/libasound_module_ctl_pulse.so ;
     }
     ctl.!default {
       type pulse
     }
     ${alsaCfg.extraConfig}
-  '');
+  '';
 
 in {
 
@@ -113,8 +105,7 @@ in {
         type = types.bool;
         default = false;
         description = ''
-          Whether to include the 32-bit pulseaudio libraries in the system or not.
-          This is only useful on 64-bit systems and currently limited to x86_64-linux.
+          This option is no longer supported, and will eventually be removed.
         '';
       };
 
@@ -227,6 +218,11 @@ in {
     }
 
     (mkIf cfg.enable {
+      assertions = [{
+        assertion = !cfg.support32Bit;
+        message = "`hardware.pulseaudio.support32Bit` is no longer supported.";
+      }];
+
       environment.systemPackages = [ overriddenPackage ];
 
       sound.enable = true;
diff --git a/nixos/modules/programs/steam.nix b/nixos/modules/programs/steam.nix
index ff4deba2bf0ae..2bde9a0999277 100644
--- a/nixos/modules/programs/steam.nix
+++ b/nixos/modules/programs/steam.nix
@@ -39,9 +39,6 @@ in {
       driSupport32Bit = true;
     };
 
-    # optionally enable 32bit pulseaudio support if pulseaudio is enabled
-    hardware.pulseaudio.support32Bit = config.hardware.pulseaudio.enable;
-
     hardware.steam-hardware.enable = true;
 
     environment.systemPackages = [ steam steam.run ];
diff --git a/nixos/modules/services/audio/jack.nix b/nixos/modules/services/audio/jack.nix
index 84fc9957b879f..f2ccd73ed382a 100644
--- a/nixos/modules/services/audio/jack.nix
+++ b/nixos/modules/services/audio/jack.nix
@@ -8,8 +8,6 @@ let
   pcmPlugin = cfg.jackd.enable && cfg.alsa.enable;
   loopback = cfg.jackd.enable && cfg.loopback.enable;
 
-  enable32BitAlsaPlugins = cfg.alsa.support32Bit && pkgs.stdenv.isx86_64 && pkgs.pkgsi686Linux.alsa-lib != null;
-
   umaskNeeded = versionOlder cfg.jackd.package.version "1.9.12";
   bridgeNeeded = versionAtLeast cfg.jackd.package.version "1.9.12";
 in {
@@ -67,7 +65,7 @@ in {
           type = types.bool;
           default = false;
           description = ''
-            Whether to support sound for 32-bit ALSA applications on 64-bit system.
+            This option is no longer supported, and will eventually be removed.
           '';
         };
       };
@@ -129,9 +127,7 @@ in {
     (mkIf pcmPlugin {
       sound.extraConfig = ''
         pcm_type.jack {
-          libs.native = ${pkgs.alsa-plugins}/lib/alsa-lib/libasound_module_pcm_jack.so ;
-          ${lib.optionalString enable32BitAlsaPlugins
-          "libs.32Bit = ${pkgs.pkgsi686Linux.alsa-plugins}/lib/alsa-lib/libasound_module_pcm_jack.so ;"}
+          lib ${pkgs.alsa-plugins}/lib/alsa-lib/libasound_module_pcm_jack.so ;
         }
         pcm.!default {
           @func getenv
@@ -217,6 +213,10 @@ in {
           assertion = !(cfg.alsa.enable && cfg.loopback.enable);
           message = "For JACK both alsa and loopback options shouldn't be used at the same time.";
         }
+        {
+          assertion = !cfg.alsa.support32Bit;
+          message = "`services.jack.alsa.support32Bit` is no longer supported.";
+        }
       ];
 
       users.users.jackaudio = {
diff --git a/nixos/modules/services/desktops/pipewire/pipewire-media-session.nix b/nixos/modules/services/desktops/pipewire/pipewire-media-session.nix
index f7a03a4a3eaf1..d7ead0464fd88 100644
--- a/nixos/modules/services/desktops/pipewire/pipewire-media-session.nix
+++ b/nixos/modules/services/desktops/pipewire/pipewire-media-session.nix
@@ -6,9 +6,6 @@ with lib;
 let
   json = pkgs.formats.json {};
   cfg = config.services.pipewire.media-session;
-  enable32BitAlsaPlugins = cfg.alsa.support32Bit
-                           && pkgs.stdenv.isx86_64
-                           && pkgs.pkgsi686Linux.pipewire != null;
 
   # Use upstream config files passed through spa-json-dump as the base
   # Patched here as necessary for them to work with this module
diff --git a/nixos/modules/services/desktops/pipewire/pipewire.nix b/nixos/modules/services/desktops/pipewire/pipewire.nix
index c3cfd46e61c21..1a3ef1e7954f4 100644
--- a/nixos/modules/services/desktops/pipewire/pipewire.nix
+++ b/nixos/modules/services/desktops/pipewire/pipewire.nix
@@ -6,9 +6,6 @@ with lib;
 let
   json = pkgs.formats.json {};
   cfg = config.services.pipewire;
-  enable32BitAlsaPlugins = cfg.alsa.support32Bit
-                           && pkgs.stdenv.isx86_64
-                           && pkgs.pkgsi686Linux.pipewire != null;
 
   # The package doesn't output to $out/lib/pipewire directly so that the
   # overlays can use the outputs to replace the originals in FHS environments.
@@ -115,7 +112,7 @@ in {
 
       alsa = {
         enable = mkEnableOption "ALSA support";
-        support32Bit = mkEnableOption "32-bit ALSA support on 64-bit systems";
+        support32Bit = mkEnableOption "This option is no longer supported, and will eventually be removed";
       };
 
       jack = {
@@ -156,6 +153,10 @@ in {
         assertion = cfg.jack.enable -> !config.services.jack.jackd.enable;
         message = "PipeWire based JACK emulation doesn't use the JACK service. This option requires `services.jack.jackd.enable` to be set to false";
       }
+      {
+        assertion = !cfg.alsa.support32Bit;
+        message = "`services.pipewire.alsa.support32Bit` is no longer supported.";
+      }
     ];
 
     environment.systemPackages = [ cfg.package ]
@@ -186,14 +187,10 @@ in {
     environment.etc."alsa/conf.d/49-pipewire-modules.conf" = mkIf cfg.alsa.enable {
       text = ''
         pcm_type.pipewire {
-          libs.native = ${cfg.package.lib}/lib/alsa-lib/libasound_module_pcm_pipewire.so ;
-          ${optionalString enable32BitAlsaPlugins
-            "libs.32Bit = ${pkgs.pkgsi686Linux.pipewire.lib}/lib/alsa-lib/libasound_module_pcm_pipewire.so ;"}
+          lib ${cfg.package.lib}/lib/alsa-lib/libasound_module_pcm_pipewire.so ;
         }
         ctl_type.pipewire {
-          libs.native = ${cfg.package.lib}/lib/alsa-lib/libasound_module_ctl_pipewire.so ;
-          ${optionalString enable32BitAlsaPlugins
-            "libs.32Bit = ${pkgs.pkgsi686Linux.pipewire.lib}/lib/alsa-lib/libasound_module_ctl_pipewire.so ;"}
+          lib ${cfg.package.lib}/lib/alsa-lib/libasound_module_ctl_pipewire.so ;
         }
       '';
     };
diff --git a/nixos/tests/installed-tests/pipewire.nix b/nixos/tests/installed-tests/pipewire.nix
index b04265658fcf4..b92bd8283ee06 100644
--- a/nixos/tests/installed-tests/pipewire.nix
+++ b/nixos/tests/installed-tests/pipewire.nix
@@ -9,7 +9,6 @@ makeInstalledTest {
       pulse.enable = true;
       jack.enable = true;
       alsa.enable = true;
-      alsa.support32Bit = true;
     };
   };
 }
diff --git a/nixos/tests/pulseaudio.nix b/nixos/tests/pulseaudio.nix
index 4e2ce679acd71..155b1115eb514 100644
--- a/nixos/tests/pulseaudio.nix
+++ b/nixos/tests/pulseaudio.nix
@@ -33,7 +33,6 @@ let
             imports = [ ./common/wayland-cage.nix ];
             hardware.pulseaudio = {
               enable = true;
-              support32Bit = true;
               inherit systemWide;
             };
 
diff --git a/pkgs/games/steam/fhsenv.nix b/pkgs/games/steam/fhsenv.nix
index 8c379959ad8b0..a8d49938b8f27 100644
--- a/pkgs/games/steam/fhsenv.nix
+++ b/pkgs/games/steam/fhsenv.nix
@@ -299,7 +299,6 @@ in buildFHSUserEnv rec {
     and then run \`sudo nixos-rebuild switch\`:
     {
       hardware.opengl.driSupport32Bit = true;
-      hardware.pulseaudio.support32Bit = true;
     }
     **
     EOF