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After creating a basic atlas and manual annotation, we can subset to certain subpopulations (e.g. Myeloid cells) and run the Neighbors-Leiden-UMAP section again on only this subset.
The resulting UMAPs can then be explored using the embedding switcher, since switching between integration methods does not make sense for this.
A reason for this is that if there is a very high number of cells, closely related cell types are not clearly distinguishable in the UMAP any more, although in the clustering it kind of works
The text was updated successfully, but these errors were encountered:
After creating a basic atlas and manual annotation, we can subset to certain subpopulations (e.g. Myeloid cells) and run the Neighbors-Leiden-UMAP section again on only this subset.
The resulting UMAPs can then be explored using the embedding switcher, since switching between integration methods does not make sense for this.
A reason for this is that if there is a very high number of cells, closely related cell types are not clearly distinguishable in the UMAP any more, although in the clustering it kind of works
The text was updated successfully, but these errors were encountered: