diff --git a/uvicorn/server.py b/uvicorn/server.py
index a3fb31b2b..34cbcbb40 100644
--- a/uvicorn/server.py
+++ b/uvicorn/server.py
@@ -10,7 +10,7 @@
 import time
 from email.utils import formatdate
 from types import FrameType
-from typing import TYPE_CHECKING, List, Optional, Sequence, Set, Tuple, Union
+from typing import TYPE_CHECKING, Any, List, Optional, Sequence, Set, Tuple, Union
 
 import click
 
@@ -54,6 +54,7 @@ def __init__(self, config: Config) -> None:
         self.should_exit = False
         self.force_exit = False
         self.last_notified = 0.0
+        self.servers: List[Any] = []
 
     def run(self, sockets: Optional[List[socket.socket]] = None) -> None:
         self.config.setup_event_loop()
@@ -112,7 +113,9 @@ def _share_socket(
                 sock_data = sock.share(os.getpid())  # type: ignore[attr-defined]
                 return fromshare(sock_data)
 
-            self.servers = []
+            # Ensure list does not have stale items if the server is run multiple times.
+            self.servers.clear()
+
             for sock in sockets:
                 if config.workers > 1 and platform.system() == "Windows":
                     sock = _share_socket(  # type: ignore[assignment]