diff --git a/packages/calcite-components/src/components/autocomplete/autocomplete.e2e.ts b/packages/calcite-components/src/components/autocomplete/autocomplete.e2e.ts
index a30764a5ab5..920b065de53 100644
--- a/packages/calcite-components/src/components/autocomplete/autocomplete.e2e.ts
+++ b/packages/calcite-components/src/components/autocomplete/autocomplete.e2e.ts
@@ -455,6 +455,14 @@ describe("calcite-autocomplete", () => {
     focusable("calcite-autocomplete");
   });
 
+  it("should set screen reader list attribute 'aria-live' to 'polite'", async () => {
+    const page = await newE2EPage();
+    await page.setContent(simpleHTML);
+
+    const screenReaderList = await page.find(`calcite-autocomplete >>> .${CSS.screenReadersOnly}`);
+    expect(await screenReaderList.getProperty("ariaLive")).toBe("polite");
+  });
+
   it("should be able to remove icon", async () => {
     const page = await newE2EPage();
     await page.setContent(simpleHTML);
diff --git a/packages/calcite-components/src/components/autocomplete/autocomplete.tsx b/packages/calcite-components/src/components/autocomplete/autocomplete.tsx
index f85ba8c51b0..210c4e759c1 100644
--- a/packages/calcite-components/src/components/autocomplete/autocomplete.tsx
+++ b/packages/calcite-components/src/components/autocomplete/autocomplete.tsx
@@ -867,6 +867,7 @@ export class Autocomplete
     return (
       <ul
         aria-labelledby={this.inputId}
+        ariaLive="polite"
         class={CSS.screenReadersOnly}
         id={this.listId}
         role="listbox"