diff --git a/test/integration/manifests/cilium/v1.14/cilium-agent/templates/daemonset-dualstack.yaml b/test/integration/manifests/cilium/v1.14/cilium-agent/templates/daemonset-dualstack.yaml
index dfec32a34e..6bd16ea442 100644
--- a/test/integration/manifests/cilium/v1.14/cilium-agent/templates/daemonset-dualstack.yaml
+++ b/test/integration/manifests/cilium/v1.14/cilium-agent/templates/daemonset-dualstack.yaml
@@ -42,12 +42,6 @@ spec:
                 operator: In
                 values:
                 - linux
-        podAntiAffinity:
-          requiredDuringSchedulingIgnoredDuringExecution:
-          - labelSelector:
-              matchLabels:
-                k8s-app: cilium
-            topologyKey: kubernetes.io/hostname
       containers:
       - args:
         - --config-dir=/tmp/cilium/config-map